LOCUS       DAORQA010000001        82367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_44_length_82367_cov_34.774310, whole genome shotgun sequence.
ACCESSION   DAORQA010000001 DAORQA010000000
VERSION     DAORQA010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 82367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 82367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82367
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44_length_82367_cov_34.774310"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PK736_00005"
     CDS             complement(<1..356)
                     /locus_tag="PK736_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31805.1"
                     /translation="MRNIKQRTTNNFLFLVALLIISNMANAQWAQMGPGAGGQEKAVF
                     LWEKTLGLFDFYVGSDVSGVWRAPDIGIADLSNPDKYIYQYISNHTMMRFVNKLHHPP
                     SDPYNFLFVANRGGVDR"
     gene            665..1507
                     /locus_tag="PK736_00010"
     CDS             665..1507
                     /locus_tag="PK736_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRC31806.1"
                     /translation="MSIENEYADKKFIDIDKIFLEKNARLHKMIPGFILRYLKKITRQ
                     EQVNYFISKYGHLRGLAFVDAIITEFKFKYRVIGEENILEHGSVMFVANHPLGGLDGI
                     SLLQVLGRHRPEVKFIVNDILTKLKNLEDLFVGVNKHGNTTREMLQELDQVFASDGAL
                     LIFPAGLVSRRTDGVICDLEWKKAFIAKARKYKKTVVPIHISGHLTNYFYGLSNFRKK
                     IGIKANIEMLYLPDEMYKQEHLNVTITIGKAIDPAIFDKTKTDEQWAQWMKDKVYELA
                     PKEN"
     gene            1574..2509
                     /locus_tag="PK736_00015"
     CDS             1574..2509
                     /locus_tag="PK736_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC31807.1"
                     /translation="MNEQKVIDPVPGELILAELNATTFLRKTNNGNNEIYVVNHRNAP
                     NTLREIGRLREVTFRAAGGGTGLACDLDEYDTNEQPYQQLIVWNITDKDIVGGYRYIH
                     CKNAIGPNGEIMLATSELFNFSEKFIRDYMPYTIELGRSFVQPKYQPTATNRTGLFSL
                     DNLWDGLGAIIVDNEDLKYYWGKVTMYLDFNRQARDMILYFMNYIFPDSEKLVTPIIP
                     LKYETDIGAFAKEIEGLSYKEAHVILNHHVRSLKENIPPLVNSYMNISPTMKTFGTAL
                     NSHFGDVEETGILVTIADIYDSKKERHVNTYQKKS"
     gene            complement(2484..3941)
                     /locus_tag="PK736_00020"
     CDS             complement(2484..3941)
                     /locus_tag="PK736_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRC31808.1"
                     /translation="MKFNFSYLRKFFMFKNSIEKLINRLSGDSDNFTIETRMFNLTAL
                     LIGSLFSLGVVANFIIGLNFWLNISIVSCIAILFYTYYQSRYKNNYTYVLPIFIVVSY
                     TMITLNWFLGAGSNGPSPIYFLLNLITLLIISPRRQYFFILAMQLITMSGLYYVDSSH
                     SSWVIQYDDENARMIDMFLSTFFCLIFVFLIMALLKISYNRERTKVKLQQNEIYDQNK
                     LILEQNENLEKMNHLKSKLFSIISHDMRSPLSTLHSAINILNETHLSESERLTIINGA
                     KERLVYTQEMLDNLLSWANSQMNGMQTSMSKVNMKHLANSVLLHLKPQYESKFINAVS
                     EIAEDVFVYADIEMIRLVIRNIFSNAAKFTSEDGNITIKAHTENDLVWITITDNGKGL
                     TPIELEKINSKTSFSTPGTNNEKGTGLGLILCKDFLHVNNGELVVTSEMQKGTTFAFS
                     LPFYSGQDENVDKQKDNIRALIRSKDRAKTFSDMY"
     gene            4135..4785
                     /gene="yihA"
                     /locus_tag="PK736_00025"
     CDS             4135..4785
                     /gene="yihA"
                     /locus_tag="PK736_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRC31809.1"
                     /translation="MNKLFGENNNHEMIIKETEFIKSATNLEQCTPEPLPEYAFIGRS
                     NVGKSSLINMLVNKKGLAKTSSSPGKTQTINFFKVNKQWMLVDLPGYGYAKVSQEERA
                     KWKKMIWDYIEQREFLMCIFILVDLRIEPQKSDMEFVNKVGEIGVPLCIVFTKADKIP
                     KTQIPKSVAIHKKEYLKHWEELPLMFISSTETKSGREEILEFIEKANEGFANRDMG"
     gene            complement(4923..5342)
                     /locus_tag="PK736_00030"
     CDS             complement(4923..5342)
                     /locus_tag="PK736_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRC31810.1"
                     /translation="MTKIFIDANVIISVLNSEYPAFPFAAKVLSLADNPNYRLFTSPL
                     SIAISFYFATKKCGVTKAHQKIILLGNKIQIVDTPEDCLKQLAKDKKIKDVEDGMQYY
                     AAKKAKCNFIITDNIEDFYFSEIEVKTASAFLALISN"
     gene            complement(5339..5605)
                     /locus_tag="PK736_00035"
     CDS             complement(5339..5605)
                     /locus_tag="PK736_00035"
                     /inference="COORDINATES: protein motif:HMM:NF039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HRC31811.1"
                     /translation="METKLTLRFDSEIIDEIKRFAESKNISVSKLTEYLFTKVTAKNY
                     KSLEDFPIAKWIDELAEPQIKYTKPGKKKKSIHKEFYESKATKK"
     gene            5702..6481
                     /gene="surE"
                     /locus_tag="PK736_00040"
     CDS             5702..6481
                     /gene="surE"
                     /locus_tag="PK736_00040"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRC31812.1"
                     /translation="MAKSQINKPTILIVNDDGITAPGINALAQAMATIGKVVIVAPDS
                     PQSGMGHAITINKPLRLDKVDLHTDYESWQCSGTPVDCVKLAVNKVLKKKPDLCVSGI
                     NHGSNSSINVIYSGTMSAAMEGAIENIPSIGFSLSNYALDANFEASKFYANKIASAVL
                     KNGLPSNTLLNVNIPNLTKSKVKGIRICRQALAKWQEEFSERLDPTHRKYYWLTGKFV
                     NFDTGDDTDEWALAHGYVSVVPVQFDFTAHHTIAQLNKIKW"
     gene            6936..7334
                     /locus_tag="PK736_00045"
     CDS             6936..7334
                     /locus_tag="PK736_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31813.1"
                     /translation="MKSLFAICLVFMLFTTCNTNPSRKIVGEWKQYYGEADETSFNTL
                     NFTYNITLTPENELKISDSASPERIFLDISFSEDILSFSTRFPGGKSSNTVHYKLKFN
                     SETKLLEGHILSNSSREQSKISWKRVKSSK"
     gene            complement(7530..7894)
                     /gene="ssrA"
                     /locus_tag="PK736_00050"
     tmRNA           complement(7530..7894)
                     /gene="ssrA"
                     /locus_tag="PK736_00050"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            8164..9360
                     /locus_tag="PK736_00055"
     CDS             8164..9360
                     /locus_tag="PK736_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC31814.1"
                     /translation="MTIGIIKEAKLHPDKRVALMPSQCVLLQKQYHNLKILIEPSNIR
                     IVNDEEYVKANLTLSIDLSKCDIIFGIKEVPPGKLIANKKYLFFSHTIKKQPHNKLLL
                     QTILQKNIQLIDYECLVDEKDNRIIGFGRFAGIAGAYNTIRLFGLKYARFNLKPPELC
                     EHKAELEKELRKAKMGNIKILVTGGGRVANGALEILGAMKMRKVTPYEYLMFNYSEPV
                     YAQIHSSDYHVHKENKPWNSEYFYDHPDEYNSSFAKFAAKTDLLLHCAFWDPKAPVLF
                     TLEDMAQTDFKIRVIGDITCDIGGSIPCTIEATTIEQPFYEWEQYERQHVKGITENSI
                     SVMAVDNLPCSLPRDASEAFGAELISKVIPSLMKDVDDDLITRASITKDGKLTERYKY
                     LSAYIA"
     gene            complement(9365..10678)
                     /gene="rimO"
                     /locus_tag="PK736_00060"
     CDS             complement(9365..10678)
                     /gene="rimO"
                     /locus_tag="PK736_00060"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRC31815.1"
                     /translation="MRTKTYKPRSVNVVTLGCSKNLVDSEVLMGQLVANDFAVTHEAK
                     RGNSEIVIINTCGFIDNAKQESIDTILHYANQKQKGKIDKLIVTGCLSERYKPELEKE
                     ISDVDSFFGTHDLPLLLKTLGADYKHELVGERLLTTPKHFAYLKIAEGCDRPCSFCAI
                     PVMRGKHVSTPIEKIIAQAETLAASGAKELLLIAQDLTYYGLDIYKERKLASLLEQLS
                     EVAGIEWIRLHYAYPTAFPMEIIEVMKQKSNICNYLDMPVQHGSTNMLKAMRRGITRE
                     KTEKLIYDIRNKIPGIAMRTTLIAGYPGETEADHAEQMKFVADMKFDRLGVFAYSHEE
                     HTHAYNFIDDVPAEVKQNRVEEIMNLQQGISLAINENKIGKVFKVLIDRKEGNYFIGR
                     TEFDSPEVDNEVLIDATKNYLRRGHFAQIKITKAEAFDLYGEVVK"
     gene            complement(10691..11188)
                     /locus_tag="PK736_00065"
     CDS             complement(10691..11188)
                     /locus_tag="PK736_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31816.1"
                     /translation="MAKYFFILILSWGFFDFASAQKKISKIDITISDNNGFQFLSQDY
                     RITHDSLFITGVSDNGKTKVDYLKRMLTKSEKKQIQLFFTKFSCDSLQKEYFNEFNSM
                     GYITYDHYPRVINMTLKKNDNYCETKITNCFVYRVSNMINYLNDFFPAEVKIFFKKED
                     FKVAY"
     gene            complement(11202..12170)
                     /gene="ftsY"
                     /locus_tag="PK736_00070"
     CDS             complement(11202..12170)
                     /gene="ftsY"
                     /locus_tag="PK736_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRC31817.1"
                     /translation="MGFFSKLFGKEQKESLDKGLEKTRENVFTRITKAIAGKSTVDDD
                     VLDQLEEILVSSDVGVTTTLKIIERLQARVAADKYIGTSQLNNLLREEISDLLQANYK
                     DLDEQNYFKPAKKPYVIMVVGVNGVGKTTTIGKLAHQFKLAGNSVLLGAADTFRAAAV
                     DQLTIWSKRVDVPIISQGMGADPASVAYDTMQAAQSRNADVVIIDTAGRLHNKVGLMN
                     ELGKIKRVMQKVIPDAPHEVLLVLDASTGQNAIEQARQFIAVTEVNALALTKLDGTAK
                     GGVVIGISDEFKIPVKYIGVGEKMEDLQIFDRREFVDSLFKETSLS"
     gene            complement(12622..12960)
                     /locus_tag="PK736_00075"
     CDS             complement(12622..12960)
                     /locus_tag="PK736_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC31818.1"
                     /translation="MLKEIKPNKELKKNKAIKSAKNFDEILDIQYGKIGVKKRDEFEE
                     RAQYFVISETLKDARLEAKLTQEQLAIKVGTKKSYISRLENGKCDIQLSTLYRIFENG
                     LGKKINLLIS"
     gene            complement(12890..13234)
                     /locus_tag="PK736_00080"
                     /pseudo
     CDS             complement(12890..13234)
                     /locus_tag="PK736_00080"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(13482..13634)
                     /locus_tag="PK736_00085"
     CDS             complement(13482..13634)
                     /locus_tag="PK736_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HRC31819.1"
                     /translation="MAKKVVATLKSGKGKEFSKVIKMVKNPSGSYSFKQEVVHNDHIK
                     DFLKDK"
     gene            complement(13659..13841)
                     /gene="rpmG"
                     /locus_tag="PK736_00090"
     CDS             complement(13659..13841)
                     /gene="rpmG"
                     /locus_tag="PK736_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRC31820.1"
                     /translation="MAKKGNRIQVILECTEHKNSGLPGTSRYITTKNKKNDPERMEIK
                     KFNPIMKRMTVHKEIK"
     gene            complement(13862..14095)
                     /gene="rpmB"
                     /locus_tag="PK736_00095"
     CDS             complement(13862..14095)
                     /gene="rpmB"
                     /locus_tag="PK736_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRC31821.1"
                     /translation="MSKICDLTGKTSIGGNKVSFSNRKTKRRFNPNLQKKRFFIPETG
                     QWITLKVSTSALRTIHKLGATAAIEKALRKGSL"
     gene            14293..14562
                     /gene="rpsO"
                     /locus_tag="PK736_00100"
     CDS             14293..14562
                     /gene="rpsO"
                     /locus_tag="PK736_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRC31822.1"
                     /translation="MILTSEAKKEIFASHGGSATNTGSAESQIALFTQRIGHLTEHLK
                     KQPKDFSTQRSLIKLVGKRRSLLNYLHNTEINRYRAIIAKLNIRK"
     gene            14916..17141
                     /gene="pnp"
                     /locus_tag="PK736_00105"
     CDS             14916..17141
                     /gene="pnp"
                     /locus_tag="PK736_00105"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRC31823.1"
                     /translation="MTVKPVTHSFDLGNGKPISIETGKLAKQADGSVVVKCGDCMLLA
                     TVVSNKDARPGVDFLPLSVDYQEKYAGAGRFPGGFFKREARLSEYEILISRLVDRALR
                     PLFPEDYHADIQVIINAISLDPNVMPDALAGLAASAAIMLSDIPFHGPIAEVRIGRIG
                     GEFVINPTPQQIETADIDIIVAGSAENIVMVEGEMSEVSEADMVAAIKLGHDAIKVMC
                     NAQLELVAMRGALEKRTYSHETNDEDLRVAIKSATYDKAYAVAIMGMADKHKRLEMFK
                     AILEEYKSTCTEEVLAEKGDLIKKYYHDVEYDAVRDAILNESKRLDGRKLNEIRPIWS
                     EVNYIPSAHGSAVFTRGETQSLTTVTLGSKMDEQMIDGAMFKGENRFMLHYNFPPYST
                     GEARPLRGLGRREVGHGNLAMRSLKKVLPPDGDNPYTLRIVSDILESNGSSSMATVCA
                     GSLALMDAGVRTKATCSGIAMGMISDGKGKFKVLSDILGDEDHLGDMDFKVTGTKNGI
                     CACQMDLKVKGLPYEVLEEALAQAREGRLHIIGEMEKTLAHSNADYKSHAPRIIELVI
                     ARDFIGAIIGPQGKIIQEIQRETKTTISIEEKNDRGYVQIFAADKNALDAALARVNAI
                     VAIPEVGGTYTGKVKNIQTFGAFVEFMPGKDGLLHISEISYARLNSMDGVLKEGDTIQ
                     VKLIEVDERTGKFRLSAKALLPKPEGYVEPASNGSRPEGPRGPQRDRPQGGGGRPPRR
                     D"
     gene            17444..17965
                     /locus_tag="PK736_00110"
     CDS             17444..17965
                     /locus_tag="PK736_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009086325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRC31824.1"
                     /translation="MKRIILLSDTHSYMDDSILKHCAEADEIWHAGDFGNMDVIEQLQ
                     KLKPVRGVFGNIDGADIRKTFPRDVRFTCEGVDVWMIHIGGYPPRYEPHTINIIKQNP
                     PDIFICGHSHILKIMRDPSNKKILHINPGAAGKHGFQTVRTLVKFTLHESQVADVEVV
                     ELGNKDIMVKNSW"
     gene            18177..19013
                     /locus_tag="PK736_00115"
     CDS             18177..19013
                     /locus_tag="PK736_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRC31825.1"
                     /translation="METNRFVWFKKWVIGRQTLQQIRKESGYSISTLKRYFYKYLDKP
                     PQWKVYPSVKVNLIIDGTYFSKDLCLIIYRDSSLKFTQLYRITNGEWYDELKEDLENL
                     HTLGVKIESITCDGHRAILKAVRSSCQEATLQRCLVHIQRMCRIWLTTRPLSDAGKEL
                     RTIISQLHLIKTKLERDYWIVALVKWYEKHHLFVNEKSFQPTSKRYWYKHKLVRRSFM
                     TIKNALPNMFHYLDNPRIPKTTNSLESFFGHLKNHLTIHRGLSESHRKKFLQWYLRFN
                     NE"
     gene            complement(19279..20274)
                     /locus_tag="PK736_00120"
     CDS             complement(19279..20274)
                     /locus_tag="PK736_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRC31826.1"
                     /translation="MKVIIPVAGIGSKLRPHTHTQPKSLVPVAGKAILSHIVEYLMQA
                     GFDDFVFVIGYLGEKIEEHIKDKYPDLKASFVVQSPREGTGHAIWTARDFINGNDEML
                     IVLGDTIFDFDFKSVINENISALAVKKVDDPRTFGVVELNDAGIITKLVEKPKMPKSN
                     LALAGVYYIKEGAFLMKCLDQMITTGNKTNDEYTLTDAIMAMVTEGVKLKTFQIGNWM
                     DCGSRIGLLSTNATMLKRLKENTEIKYRFPNTIVIEPVSIAENCRISDSIIGPNVSIG
                     EHTIISTSIISDSIIGSYSQLETATLHHSVIGSDAYLKGFSQSLNIGDSTEIDFS"
     gene            20486..21988
                     /locus_tag="PK736_00125"
     CDS             20486..21988
                     /locus_tag="PK736_00125"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRC31827.1"
                     /translation="MKKVILIISFCLISFFKLFASPGDSIFAMPYVHNMYVTFPYPNF
                     YDSLIATNVTDMYLSVNIDFNGELFQNVGIKVKGNSSFNNPSQKKSFKVDFNTFVPAQ
                     DIHGLKKLNFNNCFKDPSFMREKLTNDFLIAHNIHAPRTAYCNVYFNNQLWGIYAVVE
                     AIDDEFCKAWFGNNDGNLFQGDPQGSLQWFGAQPSAYYQKYDLENNNTLNDWTDLVNL
                     IDVINNTTNANLQAALDAVLNTNDFLKQWVCLNMFSSLDSYLGSGHNYYIYHDTLTNK
                     FQWIAWDNNEAFGSFKMQLSNLQLKNLDMYYVSNATSRPLINKMLANPAYKSLYNQAY
                     CELKDDFTNAYFDSKIDSLDLQIKSSVYADPKKFYTNSMYDSSLTYDLNITGPQGINV
                     FGLKNFVSTRALNIQNNFIANNVVCTNSIENSADNFNLIISPNPVKNSIHIISSSLCS
                     IYKLYSMDGKEIASGPCNGDIDVSNYSAGVYFLSVLINDRLYYQLVNKID"
     gene            22125..24701
                     /locus_tag="PK736_00130"
     CDS             22125..24701
                     /locus_tag="PK736_00130"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC31828.1"
                     /translation="MKGTHLNALNRSIFVSLFLLLTSTISLFSQTEKAANPPIYIAFL
                     WHMHQPIYWPYENVVTTHNTNKYPFNLLQIFNDRNGPYTNWPKDAVLQGVNNGMPHFG
                     AQVSFSGSLIENLNNISAAGLGFNNWKSNWNTIKNQTTSLGNPRIDMVGFGYFHPLMG
                     LTNYADIRRQIQAHKTMMSANFTGNYSKGIFPPENAFSTEMIPALKDEGLEWVMVDNI
                     HFDRACNGYPFSTNGNLYEPNKADLRNADPADWVALNGIWAPTQNSALWGRQPHYVSH
                     TDPNNGTVSKIIAVPTDRYLGNQDGQGGFGALSYDYVMSQLEPFNTNSNHPMLIVLHH
                     DGDNYGGGSPAYYQNNFAAFVSWLQANPTRFVCTTVQDYLDMFPPDTNDIIHVEKGSW
                     SGADNGDPEFKKWNADYNNCVSPDRNSWAAITAAQNYVATAQQIDSTQPGTVSAYNWL
                     MCGEASDYWYWDNSLGGIWDSHPTRAANQAITLSQPVLQLGTDITGPSIWSPQREPYN
                     PGKTEWNQMQSSDFTIWTYAFDVNGIQNISLEWRTDIDNVLSYENDIYTGGIGVNAWN
                     TNAMNASYIQPQTIISPIAKAKEYSAKISGQGNTMIDYYIKCTDNFGNISKSEIQHVW
                     VGPANTINHTWQNCDTSVVQSNPLPPGVSWQPAFPTTQDTITVYVGGANQGANLHWGV
                     NQWQQVNNSYWPAGSTLFNNVGPAIESPMSAPDNWGNIQIKIGPFNKPVQSVNKVDFV
                     IHYSNNTWDNNNGNDYHIPLMFVNNFDHLPDNGLNCTINYNINGSVLNLGINSQKYEK
                     LTVCIYDVNGNCIVDSTPYEIKSGHTELGIKTNGLAEGIYFLKLKSNSVDFAKKFIVI
                     RN"
     gene            25193..25834
                     /gene="pyrE"
                     /locus_tag="PK736_00135"
     CDS             25193..25834
                     /gene="pyrE"
                     /locus_tag="PK736_00135"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRC31829.1"
                     /translation="MIEKDTAHTIAEQLLQIKAIKLQPSEPFTWASGWKSPIYCDNRK
                     SLGYPKIRTFIRQSLAKLIEERFGKPDLIAGVATAAIAPGVLVAEALGLPFCYVRSTP
                     KDHGLKNQIEGDYAANQSCVVIEDLISTGKSSLATVEALRKEKIIVKGMVSIFSYGFE
                     DAVNNFKNANCVLYSLCNYEVLLEEALAKKYIKPEQLSTLRDWRISPSTWMGA"
     gene            26489..26896
                     /locus_tag="PK736_00140"
     CDS             26489..26896
                     /locus_tag="PK736_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRC31830.1"
                     /translation="MTSIKSEKVLINVPVQRVFEFLCNFNNYQQLMPEQVTDWQSDDD
                     SGTFTIKNMATIGLRIIDKIPFEKVHLEKTKAPFELTLDCLMNDMGDNFSTQLELQLD
                     AELNAMLKMMASKPLTNFVNILATNCKKVLEGI"
     gene            complement(27102..27779)
                     /locus_tag="PK736_00145"
     CDS             complement(27102..27779)
                     /locus_tag="PK736_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC31831.1"
                     /translation="MADSKETTLDIGASINKFEGYIENNKKSLIIIGGAIVAVLAAYV
                     LYTNFWLKPKELKAQQSIFAAQFNFKNDSIDLAINGKGADMGFKQIIDEYGSTKTGNL
                     AKYYLGMCYMKKDQWQNAIDALKDYDAEDDITGALALGAIGDANMELGKKDDALDFYK
                     KAADWDDNQFTAPYFLKKQAFAYELKGDYTSAITAYTKIQTNFAQSTEARDVEKYIAR
                     ATAKAGK"
     gene            27964..29931
                     /locus_tag="PK736_00150"
     CDS             27964..29931
                     /locus_tag="PK736_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC31832.1"
                     /translation="MKKATVSAKSVNKSKATAAQRNKPSIPKGISWHWWLGLAIVVVS
                     TFVAYSTCLDNDFTNWDDPGYIIENPIIKTMSGENIKRIFTEPYFANYQPLHIFSYMI
                     EYHFFGLDASGYHWVSVIMHIINTLLVAFIALLLLKDRYIAIAVSLLFGVHSIHVESV
                     AWAAERKDLLYTMFFFTSFIFYILFVQKSQTKWLIVSVVFFVFSIFSKAMAVSLVPVL
                     FLIDVLYSRKLSVKLFLEKIPYLILALILGIVSLKVTKPEDAFTPTGDTYTLVQRLFF
                     ASHNLLQYFAKSVFPYGQSGYYQYPIVGNQTLPVQYYIAPIIVLAFLGLIIYSWRKTR
                     WIVVLAGFFTATTFLILMLYTIGPTIFSERYTYISSLAVYLFIGLGVQYFASGNGPFQ
                     MSKNIGLGLIAVYSVWLIFNTRERCNVWQDSITFWNDVISKDSRIPIAYNNRGNEYKS
                     RNEFDKAIPDLNQAIAYKEDYLEPHVILGDIYRSQGKYELAREKLEKALKINAKSHQA
                     LVNLGIVNCVQGRLEDGFDNFNKAISYKPEMFEAYGNRGNYYAMKGEIDKAIVDYNRA
                     LQINPDFKDAYLNLGKTQMEQKKPQEALKYLDLFIQRGGSPGEANWLKGVVYASMNNF
                     NAALQSANLAQAAGYAVNQQQMQLWAAGKQQ"
     gene            complement(29964..31100)
                     /gene="bshA"
                     /locus_tag="PK736_00155"
     CDS             complement(29964..31100)
                     /gene="bshA"
                     /locus_tag="PK736_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HRC31833.1"
                     /translation="MKIGIVCYPTFGGSGVIATELGKALAMNGHKVHFITYNQPVRLD
                     HFHENISYHEVTIPDYPLFEFVPYENALTSKMVDVAKYEKLDLFHVHYAIPHASAAYL
                     AKQILASEGFHVPFMTTLHGTDITLVGKNPSYAPVVTFAINHSDIVTTVSESLKEETF
                     LNFKITKHIDVVPNFIDLSRFNKQRKDHFKKAIAPAGEKIIVHTSNFRKVKRTQDVIY
                     AFEKIIKKVPAKLLMIGDGPERVYCEQLCRDLKVCDDVRFLGKQEMIEEILSVCDLFF
                     MPSETESFGLAALEAMACQVPVISTNSGGMPELNVQGVTGYLSNVGDIDDMAKNSVDL
                     LLNEEKLLQFKKNALAHAATFDIDKIRPMYERLYEQCLEAQEIK"
     gene            complement(31225..32379)
                     /locus_tag="PK736_00160"
     CDS             complement(31225..32379)
                     /locus_tag="PK736_00160"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC31834.1"
                     /translation="MISRLILPTAKETLRQFRAVCITGPRQSGKTTLSKKLFAGKPYV
                     NFENPDVQKLADESPRAFLHKYRNGAVFDEVQRVPHIFRYLQGVLDNNNKRGQFILTG
                     SNNFLLQEQVSQSLAGRAGYLSLLPLSYAELSKTKYATSEANKNILTGGYPEIWNQKL
                     SPGIWLNSYMQTYIQRDVRLLRNITNMAAFNRFVYLCANYAGQILNRDELAKQTGVDT
                     KTILAWLGLLENSYIIFQLQPWYNNLNKRIIKSPKLFFYDTGLLCHLLGIKSVTALQQ
                     NNAYGAIFENWIISELRKNNFNSGLNDGMYFFRDSTGNEVDLIMERDGETTAIEIKAS
                     KKFDNSMVRGLNYWQKYQPKSHCMLIHGGTTHKMMNDKLSVLPWTEIANI"
     gene            complement(32802..32954)
                     /locus_tag="PK736_00165"
     CDS             complement(32802..32954)
                     /locus_tag="PK736_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31835.1"
                     /translation="MAEQRHGDDGLWGHKPWLARLGLRNFGNNEKPPASKFRRNEIFV
                     AVFFHA"
     gene            complement(33307..34677)
                     /locus_tag="PK736_00170"
     CDS             complement(33307..34677)
                     /locus_tag="PK736_00170"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRC31836.1"
                     /translation="MENIKEYINKNEQRFLSELMEWLRIPSVSADSKYKDDVARAAKF
                     IAEKFTIAGADKVEICPTEGHPIVYGEKIIDPKLKTVLVYGHYDVQPADPVELWESPP
                     YEPQIRDGKIFARGACDDKGQVYMHVKAFEMMMQMNQLPCNIKFMIEGEEECGSANLG
                     TFVKNNKEKLKADVILISDTGLVANDIPSIDVGLRGLSYIEVEVTGPNRDLHSGVYGG
                     AVGNPIQILCKMITDMKDENNHITIPGFYDDVLELNDAERTELNKTPFDLEEYKTDLG
                     VKDVLGEKGFTTIERTGIRPTFELNGIWGGYIGEGSKTVLPSKAFAKISMRLVPNQKS
                     DKITELFTKYFEAVAPDCVTVKVHPHHGGEPYVCPTDSVAYNAAAKAMETTFGIRPIP
                     TRGGGSIPIVALFERELGLKSVLMGFGLDSDLIHSPNEHFGVFNFLKGIETIPHFYMN
                     YAEAAK"
     gene            34816..38325
                     /locus_tag="PK736_00175"
     CDS             34816..38325
                     /locus_tag="PK736_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC31837.1"
                     /translation="MKKIKQQLIKSVCCFFGLILIHVSAIAQPINDECYLATPIVVPQ
                     SGQVCINASNANATPDAITPNTCNQPTGGNEVWFTYITTGTNNLIAVSPSGGTPAQTL
                     ALTIPTGGCAGNIPLACNASATLNGTAIINQSFPVGTQVWFEVTALTSDGDFTICITS
                     ADNPPAYAPNVGDSCTNAIQLCNKQSFTTLGAPGFANSGAFLTCYGFGALNDIYYKFD
                     VGKTGTLEWLATPSGAINLDWEVLDITGGCPGVSIACNYTYVPPGGQSTGMQDTSTTP
                     CGNIGHICQSAIVTAGNTYVIHLNNTSFLPGVGFTISFGGTFEMAPFPSYSLTNSIGC
                     GTLTTSIVDSSVGSTNYNWVFGNGNTFAGANPPAQTYIAPGTYMTSLVVSNASSGCSH
                     VTSVPVVVKEKPTADFILTDTTICIGQSTSAVYVGTATPGATYTWNFSGANVTSGTGQ
                     GPYTLDWPTVGSKTITLYVTENGCTSDTITKTVVVSAPPVASFSLPATACTGDTLLVT
                     YTGTVIPGATFFWNFGNGFSAGGNGNDTVLITWPTAQTDSISLTVSAGGCLSTILYHT
                     IVISDKPTSTFTLPDSTCTNNTITLNYIGTGTVAAVYNWNFAGGNALPGGTVQGPHNI
                     TWVGSGLKQITLSVTENGCTSSITKDTVYVMPGPDASFSLSDDTLCTGETVTVTYTGT
                     TPGAIFNWNFSGANILSGSGAGPYVLDWPTTGVHVVSLLMSFNSCTDSIADTIYSGLT
                     PFAFVGIDKTYCSGDSVTIGAPAVPGITYNWSPAFGLSSATVSQPTVNLTNTGGAAIV
                     QNYILTTSNLLCTANDTVQISVTANQPANISLVGNNFTQCIANNDFDFSLSGPLVPGA
                     SINWTFGAAASPLTSTNAIVNNVTYNAVGTYIVQLITSANGCPADTDTVTVTIQDAPK
                     VKFGADTLIGCPPLSVSFTDSSTAPPGSTYVWDFGNSDTSTLENPNYTYTASGLYNVT
                     LTVTSPNGCSASSVVNNYINVTQEPTAVLEADPNITTILQPNIQFLNVSPYADSCYID
                     FGDGTFFNGCGNIEHIYGDTGTYIVTLYTMSAGGCVDSTEITVIIKPFFTLFIPNSFT
                     PNKDDLNELLKVYSEGVKEFSIGIFNRLGQEVYGTLNKDFAWNGYDTNGKPLPEGNYV
                     YIIRVKDVIGKRHYKQGIVSIVR"
     gene            38551..39147
                     /locus_tag="PK736_00180"
     CDS             38551..39147
                     /locus_tag="PK736_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC31838.1"
                     /translation="MGSSMQFNPLTDNELIQQYLKGSDDALRNLIERHKRKIFSTIYL
                     LVKNRDLADDLFQDTFIKVINTLRGGNYQEEGKFLPWVLRIAHNLVIDYFRKQKRMQM
                     VHDTEEYSIMDTLRLTDDNVEDKMVQDQILNQVKLLIDELPEDQRDVVIMRHYKDMSF
                     KEIAETTNVSINTALGRMRYALINLRKIIEDKKLMLEK"
     gene            39283..39513
                     /locus_tag="PK736_00185"
     CDS             39283..39513
                     /locus_tag="PK736_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31839.1"
                     /translation="MSKISTTDNLILYYFNETAMAETVLVQNAIDIDVVVRDEYEEIK
                     AAFDILDECIATPKQSSIEAIMSYSKLTQCLS"
     gene            39729..41273
                     /locus_tag="PK736_00190"
     CDS             39729..41273
                     /locus_tag="PK736_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC31840.1"
                     /translation="MPKIIKKIKFLFGILFLCQASSALLAQGLNHNWLLGYLDAPYLP
                     YSPSADATLQFTKTSYSITGTSRLMKFRATQANISDSLGNILFYSNGGWIADATGDTM
                     INGSGLSPSIFSTVYSDYGFPPPSMQIALPFPDSNNVYILFHQTSEPLESNSVELYYS
                     IIDMKQNNGKGVVTQKNLIAFQDTILFGIHACKHANGRDWWIVTLRDSSDVAIKLLFT
                     PNGIAQVDTQHLIVPLHHNGYTQPMCFSPDGKKFAYIVTTQTGNSNYPSLRDLRIFDF
                     DRCTGLFTNSFHFDLNDTFPGTCLSFSSNSRYLYTAKYFRISQFDLLSASIQASRQYV
                     ADYDTFFMGGYTDFANMYLGANGKIYISSTSTTIYFNVIDSPDSFGVSCNVLQHSLLS
                     PTFLFFNHVNHPNYYLGCDTSLGCPCLSSTSLTENGQHDFRFRVYPNPVVNNFVNIGY
                     ILPQNKSGILKLYEVNGKQLYTQGLPPWSNEQSIILPKLTNGIYNMRIESGGYFKSKN
                     VVVMHE"
     gene            41384..41782
                     /locus_tag="PK736_00195"
     CDS             41384..41782
                     /locus_tag="PK736_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HRC31841.1"
                     /translation="MYSGAKPIHFRMAKELRKNQTPAEEALWKVLSGNTMSGFKFRRQ
                     HPISNFILDFYCHQLKLGIEIDGDIHSETMQAEYDIERTKKLDELKIEVIRFTNEEVL
                     NEISNVLNTIEFEIKKRKQKSDNEPSSHVR"
     gene            42110..43258
                     /locus_tag="PK736_00200"
     CDS             42110..43258
                     /locus_tag="PK736_00200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC31842.1"
                     /translation="MINPRLSFWEYDQYFKNIDVAIIGSGIVGLSTALHIKTQSPLLK
                     VVIVERSFLPYGASTRNAGFACFGSVSELLDDMTNRTSDEVFALVQKRYKGLKLLRKT
                     LGDKNIGYHELGGYELFTHDDNFLFQQCESAIENFNIEFKDITNVESTYTLTDEKISK
                     FGFDGVSHLIWNKCEGQIDTGLMMESLLEKVRNLGVTIFNGIEIEKWEEAENSVMVHC
                     NDGFYFEAKKMIFCNNGFAKTILPQLDVQAARAQVLITNEIANLPFEGCFHYDKGYYY
                     FRNIGKRVLFGGGRNLNFAEEATTEFGLTELIQNRLEEILKNTILPNHEYEIEQRWSG
                     IMGLGNSKSSIVKPLTKRTYCAVRMGGMGIAIGSLIGEEAAEMIIQEM"
     gene            complement(43268..43579)
                     /locus_tag="PK736_00205"
     CDS             complement(43268..43579)
                     /locus_tag="PK736_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRC31843.1"
                     /translation="MQENNNNNQLNIELSEEVAEGIYSNLAIITHSNSEFVVDFVKVM
                     PGVPKAKVKARILLTPQHAKRLLNALADNISKFEKMHGVIKETEGFNLPMNFGGPTAQ
                     A"
     gene            43652..45037
                     /locus_tag="PK736_00210"
     CDS             43652..45037
                     /locus_tag="PK736_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31844.1"
                     /translation="MKSETNLSPQFIEQMKLLLKENAQSFFDSLHTSPPTSIRLNPDK
                     VASTKIPFEKIPWCEHGYYLPKRFSFTTDPFFHSGAYYVQEASSMFLHHILKFLQIEN
                     KPKRILDLCASPGGKSTLIASVMHDDSLLVSNETVSSRVGALIQNMMKWGNANVVVTN
                     NDAAAFGKMKNFFDVIVVDAPCSGEGMFRKDDFAVQQWNPNLVLQCEQRQKNILNDVW
                     DALTPGGFLIYSTCTFNKNENEDVLKHFIENNDCTSFEISANQLENISSSFDDKIFAD
                     RFFPHKTKGEGFFISVLQKTSSTIVTSEEKKIKKHDFKKHLFLFLSPNFLVAVLNGFT
                     IGVNQLHAEMIANMVSKLNVKKFGVCIGNQVGSEMRYHHHLSMWPQLNNSEFDFLDLN
                     YEDAIKFLRGQTDINCDSGKGWIIARYKNQNLGFAKNLGNRINNYYPKELRIMNPSLQ
                     ANDQEIVSTFF"
     gene            complement(45053..47164)
                     /locus_tag="PK736_00215"
     CDS             complement(45053..47164)
                     /locus_tag="PK736_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRC31845.1"
                     /translation="MTSKKSILFALVLFNLNNLNVDAQKLEYPTTKKTEVFDEYFGTK
                     ISDPYRWLEDDNSAETADWVVQQNKVTNDFLATIPFRDSLKSRMTQLWNFPKYLPPFK
                     CGQTYFYIKNNGLDNQGSLWYQRSLLHVATLFLDPAKVDKTGNTFLSDYTPSRNGEYL
                     AFKVAKAGSDWQQIRVKEVRTAKTLADSIDWAKFSNIAWQGYGFYYSRYDAVTTGKYS
                     GKNENHKVYYHALGTTQDKDILVYHDDEHPLRTYSATTTEDEKYLLLSISESTSGNAM
                     MIKNLYKNETSFTPLFEGFSDNYNLINNEGDKLYFLTTNNAPKGRIIVVNAANPADKN
                     WQDIVPQQDDVMQSAKFAGGKLIVKYMHNASNILKVYDLNGKFENDIKQGTYTTIDAM
                     EGNKEDSVFFYSAVSLTSPPAIYKYLIDTKKTLLHFQPKLKYNPDDFVTKQVFYNSKD
                     GTSIPMFIVHKKNVVLDGKNPLLLFGYGGFNISKTPEFKPERITFLEQGGIFAMPNIR
                     GGGEFGEAWHKAGTILKKQNVFDDFIAAAEYLIKNKYTNKDKLAISGRSNGGLLIGAC
                     MTQRPDLFRVAIPVVGVMDMLRYNKFTIGWSWKSDYGTSENQNEFDALIKYSPLHNIK
                     PGKIYPATLVTTGDHDDRVVPAHSFKFISTLQEAQKGDLPTLIRIDTNAGHGAGKPTS
                     KLIDEQTDVFAFMMFFLGMKY"
     gene            47437..48714
                     /locus_tag="PK736_00220"
     CDS             47437..48714
                     /locus_tag="PK736_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31846.1"
                     /translation="MFSKLKNKLKSVFSNCIFSKEESDVDYVFFDTVAKVPVDDWNRI
                     FKNSESCLCQSLAYLQCLESSITQPNLLRYLIIYKSQQPIAAFYFQLYDVNNMSVKKL
                     LNDQYFKALLDKLKGLNDEKLFGTNDKKRFVVVNGNLFASESSGFACNEDPESLNAWQ
                     WEKVLDLLMNSVNENSSLLCCVSKDFQTTDVVLAGKLQSAGFKPFQVDPEMNLNIQWK
                     NFEEYLASLTTKYRTRIKSVFDKSNEIEVEELSAVGIKKYFGQINALLKNVFENAPVR
                     LASIEANFFICQKEKFDDNYIFNILKLNNEIVGFYTALRNNKTLTAQFVGFDYALNRS
                     HNLYYRMLIEYTKLAIDKKCMLLQFGRDALEIKSTLGATPSPLQLFLFVPGMFASFLV
                     DPFLKIATSREWIQRQPFKQINQPSTLDLKHSA"
     gene            48707..49222
                     /locus_tag="PK736_00225"
     CDS             48707..49222
                     /locus_tag="PK736_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRC31847.1"
                     /translation="MRKLSDNQKRFIKTLKPGQVVNAKIMSFSPHGTFVKIGIVNGLL
                     RNSDISHDPVHDAEEVLAINQRRDVVILKVDYENYTVQVGIKQLLTDPWTKFLAKYKV
                     GSGVVATVKNIMEYGVFFHLIPGISGLLHNSEIKKHNLLPSSFEVDEVHQLLIKNIDE
                     ETRKIELEIET"
     gene            complement(49319..49669)
                     /gene="secG"
                     /locus_tag="PK736_00230"
     CDS             complement(49319..49669)
                     /gene="secG"
                     /locus_tag="PK736_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRC31848.1"
                     /translation="MFTFLAIALLITAILLGGIILIQSSKGGGLAGNLSGGNQYMGVQ
                     KTTEGLEKITWGFATGLMVLAIFAAFALPAHNKTVAKDTVKESKLKDNVSSMPQTMPP
                     AGAQPSTTANQPAK"
     gene            complement(49671..51575)
                     /locus_tag="PK736_00235"
     CDS             complement(49671..51575)
                     /locus_tag="PK736_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31849.1"
                     /translation="MESGIIIDIIYNSSKQNDVAIDTCNNIIEQYPYFQMAHMLKSKI
                     LHTENKIDFAKAIKNAAIYAPDRSKLHDLIQTKNEPKHAFNKSNVEKWEMESKAIPLI
                     KEEEQLSDVENVEIEKGDFIIEAINTDEIIDDTNIVIADNDESLLAKLVQAEAKEPIE
                     PALIDEESHAEDVGHHPVFEHVDALIVEDILNENETDEVDSEVEVAEAIISDRISTVE
                     NNENELSDPEAIARSEKVDEIIAHIDATLPEALDRDANEELINQRLKELFSEDELAVS
                     ENNVVQHKEEEIVVDENDFSQPTATENIPLEVAVASIDQPNVVEEFEENVTAEIVISQ
                     ETEPQLSDEDFFELIAIANKESVDFDEYESDFHAVTEDEKFEIEALMSDGLVLNTNDT
                     LPEVADGLPGENINDSDSQPEIIDDIIVAEPTDNDHVFVEEEIGLLNIELESAYFDIE
                     KIATQADKTETSTIEEQAFETHAEQEEINLNNNVTEQHDFFDWLTVFQKKESKNNWNQ
                     EVASNEISENDDDHGLNDLPLQNEHQGTKSRFSSQNANVNKDEIIEHFIKADPRIEPL
                     KAEFYKPSNMARKSVEWDENLVSETLADIFRQQGLNEKAIKAYEKLSLAFPEKKHFFA
                     EIIKTIKNTD"
     gene            51979..52479
                     /locus_tag="PK736_00240"
     CDS             51979..52479
                     /locus_tag="PK736_00240"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC31850.1"
                     /translation="MNTRSIFYYNILIDMADNNQQIEDIDSMDVQTLLNIATHNNAVS
                     YMAQSALMLAGKAHFDDWLLRPAYGQSNRTLAEAGENKTDLFAFDIYPNPANSEFIIQ
                     LPNPELVSKIYIINNIGVTVMEYNENSLQKYNTIEANRLTNGIYLIRVINKDGFSCSR
                     PVVITN"
     gene            52561..54009
                     /locus_tag="PK736_00245"
     CDS             52561..54009
                     /locus_tag="PK736_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31851.1"
                     /translation="MTLICCLLCNFATAQYTFNKQYDYYVDASSNVIETNDGYISAIS
                     AWYNFNTIYLVVNKVDKKGDTVFQKVYGVLDNDNYEVYTIQQVEDSNYIIGTLYSDST
                     GYADNFLIKVDKHGDSIWMRRHHAPQGYLYFPGHVNIASNGDYLIASNIMDSIGDDVN
                     GGIAKYDSSFNLSFITYFGGIYDDNLLDAIELPDHSIMAVGWTRSYGFGNPSNRDIYL
                     VKTDSAGNLIWQKTFGNSDFESANTICPTLDGNYIVTGGHRPGGQPNPYKTYLIKVDT
                     AGKKIWEKTFLFSGQNDCHRLRELANGDLIGCGVGTYNGQEGGLIFNLDSTGKEQWHR
                     LYTVNSSNAIFRDIRATTDGGFICAGFAFVGNSGNQDALLVKVDSLGCDSAGCPLYTA
                     IEETTSAPSITNEVRCNPNPCGDFAHIHTRMLSIYGLVLFDINGREVAIRHRTTSHNS
                     IQLDTSVLTVSIYVAKILTDGGDLYVRVVKGN"
     gene            54030..54191
                     /locus_tag="PK736_00250"
     CDS             54030..54191
                     /locus_tag="PK736_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC31852.1"
                     /translation="MKKMPNIYNSNSHEIDFTISNKNEIDITEQPAGLYFVKISDGKK
                     VYVAKVMKE"
     gene            complement(54429..54962)
                     /locus_tag="PK736_00255"
     CDS             complement(54429..54962)
                     /locus_tag="PK736_00255"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HRC31853.1"
                     /translation="MEKLFYISKKSHIGLYKLILGLSIVMLINGCYSFKAGQIPKEVK
                     TYSVQFFPNRASIVNPVLSQTFTEKLKLKWNNESSLSLKSAEGDYAFEGYISNYANGP
                     VAIQSNQTAAQNRLTITVNVKFINSKDEKANFETSFSRFADYSSSLNFATQEQKLVDN
                     IVGQLVDDIFNKCTSNW"
     gene            complement(54962..56191)
                     /locus_tag="PK736_00260"
     CDS             complement(54962..56191)
                     /locus_tag="PK736_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRC31854.1"
                     /translation="MQVQDIKNRFGIIGNSPLLEYAIDTARQVAPTEISVMIMGESGV
                     GKEVFPQIMHHYSPRKHGPYIAVNCGAIPEGTIDSELFGHEKGSFTGAHEARKGYFEE
                     ANKGTIFLDEVGDLPLATQVRLLRVLEAGEFIKVGSSKIMKTDVRVVGATNVNMKDAM
                     SRGKFREDLYYRLCTVPIYVPSLRQRKEDIPLLIRKFSIDFAEKYRMPMLQISPDAMH
                     MIVDFPWPGNIRQLKNITEQISILEKDKNLTAETLLKYLPRDNQAGLPVLFRDEKGGI
                     SDFGAEREMLYKILFDMRKEINELKRVAYEQGASMEPISRGENFPVSHSNMPSDKGNI
                     NPYITTPDGFTVQTKPGYDMKPHEVVEESLSIQDKEKELITKALQKHRNRRKNAAKEL
                     GISERTLYRKINEYGIK"
     gene            complement(56201..57571)
                     /gene="miaB"
                     /locus_tag="PK736_00265"
     CDS             complement(56201..57571)
                     /gene="miaB"
                     /locus_tag="PK736_00265"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRC31855.1"
                     /translation="MELPTNFGDEHKRKLYIESYGCAMNFSDTEIVASIMSGNNFTTT
                     RNVEEADVVFLNTCAIRDNAEQRVWGRLRELKLVKKNNPDLIVGVLGCMAERLKTSLL
                     ESEQLVDLVVGPDAYRDLPNLINKVDSGQKAVNVLLSREETYADISPVRLSTNGISAF
                     ISIMRGCDNMCSFCVVPFTRGRERSRDFDSIVNEARLLFEDGYREVTLLGQNVDSYKY
                     TNESNGEFCNFANLLEKVALVNPLLRIRFSTSHPKDITDEVLHTIVKYENICNYIHLP
                     VQSGNSRVLKLMNRTYTREWYIDRINAIRKIVPGCGISSDVISGFCSETEEEHKDTLS
                     IMQYSQYDYSYTFKYSERPGTLAARNYKDDIPEEIKNRRLSEIIVLQRELSHQSNKRD
                     EGKTFRVLVEAVSKKSTDDMCGRNDQNKMIVFPKQNTIKGDYVYVKVHGCTSATLLGE
                     IVEKGS"
     gene            58050..60467
                     /gene="topA"
                     /locus_tag="PK736_00270"
     CDS             58050..60467
                     /gene="topA"
                     /locus_tag="PK736_00270"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRC31856.1"
                     /translation="MASNLVIVESPAKAKTIEGFLGKDYIVKSSYGHVRDLAKSDKAV
                     DIANNFQPNYEISPDKVEVIKELTKLAAKATSVWLATDEDREGEAISWHLFEALNLHG
                     KDTKRIVFHEITKKAITDAINNPRQIDKNLVDAQQARRILDRLVGFELSPVLWKKVKP
                     SLSAGRVQSVAVRLIVEREREIEAHNSTSSYKVVAQFNVKDNKGSNVLLKAELPTKYN
                     TREEAQQFLEQCKGATFTIGNLETKPGKKSPSAPFTTSTLQQEASRKLGFGVAQTMVV
                     AQKLYEAGKISYMRTDSVNLSDFALDASMQQIETSYGNKYHQRRVYKTKSANAQEAHE
                     AIRPTYFENSTTDGESNERRLYELIWKRAIASQMADAILERTIATINISTLANNFIAT
                     GEVIKFDGFLKVYMESTDEDSEDEDTKGLLPPLSIGQVLDLNNITAAEKFSYPAARYT
                     EASLVKKLEELGIGRPSTYAPTISTVQKRGYVIKEDKQGRERKINILTLQNDKIEASE
                     KTEITGAEKSKMFPTDIGTVVTDFLVKHFDDVLDYSFTARVEDEFDEISRGEIKWQDM
                     LREFYEPFHKDVLITEKEGERASGERILGVDAATGKTVLVRIGRFGPLVQLSPLTEEE
                     KPLFAKLRPGQRLETITMEEAMDLFRLPRTIGKFEDEDMVVAIGRFGPYVRHNGAFYS
                     LAKTDDPYTVDEERALEIIKNKRQADIDKVIKVFAEDENTRILKGRWGPFIAHGDQNL
                     RIPKGTEPSAITFAEILEIAKTAPPSKPRFGARSKAAADTKAKPFKKTAIKKAPAKKS
                     AAKKKAL"
     gene            60683..61855
                     /locus_tag="PK736_00275"
     CDS             60683..61855
                     /locus_tag="PK736_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HRC31857.1"
                     /translation="MSLSELRNFVKPSVVYKNNEFAKGTIGDKVIQFGTGEFDFDTTE
                     PCIALLGIDEGRGCKFQCDYAMGSDVIRFEIASLMQHYGKHQIIDLGNLIVGKTVNDT
                     YMAVSLLCAELIRNNIFPIIFGGSQDLIYGQYGAYKQLAQIINITNIDARFDLGEPEE
                     GLNSQSYLGHIIMEQPNYLFNYSHIGHQTYFTGEDAIEKMTKLYFDSYRLGYVRKEMQ
                     DIEPVFRNTDLLSFDIASIRNCDAMATYLAAPNGLYAEEACQLTFYAGLSEKVTSFGV
                     HNYVPSLDINQNTAKLVAQMVWYYIDGFNKRKNEFPAFNKNGFLKYIVSLSEIDNELI
                     FLKSLTSDRWWMEIPVPDGQNKVKRQHLVPCSYKDYLSACNNEMPERWWQTFKKLS"
     gene            61962..62885
                     /locus_tag="PK736_00280"
     CDS             61962..62885
                     /locus_tag="PK736_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRC31858.1"
                     /translation="MKKITCSFLLIFGSILYTYGQQDPQFSQNMHNKLFVNPAYAGSS
                     GAFCGSLLYRSQWAGYSGDGQPKTGLISLDAPVDVLHGGLGLTLFQDQLGYEKTFGVK
                     LGYAFRFNVGATGKLGIGIDAGYMQKSIDGTKFNPNDPGDPNIPGSVKANVVPDLGAG
                     IYYNNEKLYIGFSSSHLAEGSFDLGVTKYKLSRHYYGTAGYRFDLSPSIGLTPSVFVK
                     NDGATTQLDANVLAHFNEKFWVGASYRIEDAFVLMAGFNIFNGLKIGYSYDFTSSELK
                     NYSDGSHEIMLGYCFNPKKKPIFRNINVRYL"
     gene            63013..64275
                     /locus_tag="PK736_00285"
     CDS             63013..64275
                     /locus_tag="PK736_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRC31859.1"
                     /translation="MKKVFFFICITWLFYGCGGGNKGQLTGVLNRPSWYQPDPYGMVY
                     IPSGSYAMGPSDQDVPYAITSQTRNVTVHAFYMDDTEITNNEYRQFVDWVRDSIAHRL
                     IGEEHIINEGEYTEMLNWRKRIKWDDEDNVEKLNEMFLPESERFYKRKEIDSRKLNFE
                     YYWIDLKTAAAKVNREQGKTDRSVFIKKDIINVYPDTLTWIHDYTYSFNEPMTQMYFW
                     HPAYDDYPVVGVTWKQARAFCIWRTMLQNNYLDAIEEPYHQDYRLPTEAEWEYAARGG
                     LEQSPYPWGGPYIRNSLGCFLGNFKPMRGNYTDDGGFYSVKVTSYWPNDFGLYCMAGN
                     VSEWCRDAYEASSYNFTHDLNTDYDYEYKEDDLPAMRRKVIRGGSWKDIGYYLQTGTR
                     SWEFGDTSKCYVGFRCVQSFLGRDKADF"
     gene            64352..65164
                     /gene="gldL"
                     /locus_tag="PK736_00290"
     CDS             64352..65164
                     /gene="gldL"
                     /locus_tag="PK736_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HRC31860.1"
                     /translation="MASGGFTQSKTFKNIMVKLYGWGAAVVIVGALFKIQHWDGADIM
                     LILGLGTEAVIFFVSAFEPPHQEVDWSLVYPELAGMHEPGAQKKKPLAGAKDPVAQQL
                     DKMLTDAKIGPDLIQSLGTGLKSLGDNAGKMADISNATVATSEYSANIKNASKSVSEM
                     TTAYGKATETMNSFNSSSSDLKTYNEQMGKASKNLGALNAVYELQLQDSNKHLKETSK
                     FYEGINTLMGSLSASINDTQKYKEEVGKLATNLTKLNTVYGNMLSAMSGGTK"
     gene            65205..66797
                     /gene="gldM"
                     /locus_tag="PK736_00295"
     CDS             65205..66797
                     /gene="gldM"
                     /locus_tag="PK736_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HRC31861.1"
                     /translation="MSGGNISPRQKMINMMYLVLTALLALNISKEIINAFVTVNSSLE
                     TTNKNTDIKNSMSFASFEKSMQNDPVKTKPHFDRAKIAQKLSDDLVKSIDKLKDALIA
                     KSEKLQPGQPIPKAENIKNKEDFDHPTTIMCGKSHDGKGAEATKLKDQLLKYKKDIVA
                     TLEAKDQAEISKRLDEIFNTSDPDPKSEIVTKDNKRTWEMATFYHNPVVATVAMLSKI
                     QSDIKNAESEVLNKLLTNINATDLKFSDVIAKVVAPTSYVLTGQQYKADVFLAAYNKT
                     ADPEIYIGGSKLNVEEGMGKYVVTAQGEGLKKWGGVIKVKKPDNTYSEYPFDAEYITA
                     KPAAVISPTKMNVIYIGIDNPVSISVPGVPNDKVSVSTEGGGLSLKKVTGEQYIARAT
                     TQAQDAKITCIGDFDGKKITMGAQTFRVKTVPNPIAKVGGVTGGPMQKSTLMAQPGIT
                     PVMENFEFELFFKITKFTFSMYGKGKDPIDLSSSSNQITQQMKDAMARGKAGDKVYFE
                     NIYATGPDGSTRKLAPVNFVIQ"
     gene            66818..67666
                     /gene="gldN"
                     /locus_tag="PK736_00300"
     CDS             66818..67666
                     /gene="gldN"
                     /locus_tag="PK736_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HRC31862.1"
                     /translation="MVKRLFFVFVGGLLSLTGYSQNVLDGAYLRENSPALKVVPLAYL
                     READVMWSKKIWRYIDCTEKINLPLRYPLEDMTNDRKSLMQLLLTNVNEGALTAYDVL
                     DDEFTKRMTLDEIKERCGSKKDTQQITDLDPPYLTRDTVVEKKFSTDKVVGYRLKEEW
                     FFDKQRSVLDVRIIGLAPMVFAEDEAGNKIEGGIKKALFWIYYPEARNILVNYETFNR
                     GNEAERRTFDEILMKRMFNSLIYKESNVYDRRIGDYTVAPIDAILESERIKNELMILE
                     HDLWEY"
     gene            complement(67739..68527)
                     /gene="mazG"
                     /locus_tag="PK736_00305"
     CDS             complement(67739..68527)
                     /gene="mazG"
                     /locus_tag="PK736_00305"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRC31863.1"
                     /translation="MMQHTNYQPAQEAFARLLQIMDELRAQCPWDKKQTMESLRHLTI
                     EEVYELADSILDKDLNEVKKELGDILLHIIFYAKIASETNAFDITEVINAICDKLISR
                     HPHIYSDTIANDEETVKKNWEQLKMKEGNTSVLQGVPSSLPSLIKAMRIQEKARGAGF
                     DWDNPAQVWEKVIEEMNEFKCEVELGNKNRMAAEFGDLLFSLINYARFHEINPEEALE
                     LTNRKFINRFKYMENEVRKLNQKLENMTLAEMDVHWNEAKKIFK"
     gene            complement(68524..69414)
                     /locus_tag="PK736_00310"
     CDS             complement(68524..69414)
                     /locus_tag="PK736_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HRC31864.1"
                     /translation="MIEFIENENIRVKIKVIGAELCGIFHKKNEIEYIWQANEPWKKY
                     APILFPIVGQLKNNTYHFKKETYTLPRHGFAREKLFRLAFITPNEIHFEISDDEITLP
                     IYPFYFHLLVKYLLAEDDTLHITYEITNRSSEAIYFSIGGHPAFNVPLSESENFEDYF
                     IQFNEKENLRCFPLQDGLLQTKSLQLDSKNIDTEENLPTIKLSKELFDKDALVFKNLK
                     SKEISIRNKKNNHSVTMNFDGFKYFGIWSAKNANFVCLEPWLGIADNLDSSQQLIEKE
                     GIIILPAQEVFKCTYSIKVK"
     gene            complement(69514..70503)
                     /locus_tag="PK736_00315"
     CDS             complement(69514..70503)
                     /locus_tag="PK736_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRC31865.1"
                     /translation="MGNLLYWIIGLALIFDFINGFHDAANSIATVVSTKVLSPLSAVI
                     WAASFNFIAYWVFELHVADTVSKVVKPEGLSLTVIGAGLLAAIFWNLITWWKGIPSSS
                     SHTLVGGFAGAGIAFAGFDIVKLDKVMPMIYFIVLAPLIGMFMGNLISVITINICKKF
                     TPSKVDKVFRRLQLISAAAYSLGHGGNDAQKVMGIIAAAYIANGTITDIHGLPLWVPF
                     ACYSAIAIGTMFGGWRIVKTMGQKITKLTPFEGFSAETAGAITLFGTGYLGIPVSTTH
                     TITGSIIGVGITKRVSAVRWGLTFNIIWAWVLTIPVTAIIAMLFYHILKYCGL"
     gene            complement(70506..71057)
                     /locus_tag="PK736_00320"
     CDS             complement(70506..71057)
                     /locus_tag="PK736_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRC31866.1"
                     /translation="MVNYEMSRLNNNERVKEFLFQIETLEHKGDQITHEIFKELGSNF
                     ITPFDREDIHALASAIDDICDHIHGAAKRMDLYKIEKSTVPIQKLSEIIYKSAEELNI
                     AIIELQKKGNLNKVMESCVKITGLENHADDVFEMAIAQLFEEEKDPIKIIKMKEVLST
                     LETATDKCEDAANVLESIILKHN"
     gene            71565..74297
                     /locus_tag="PK736_00325"
     CDS             71565..74297
                     /locus_tag="PK736_00325"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRC31867.1"
                     /translation="MNALIPMDKSIRTGVLPNGMKYYIRKNNKPENRAEMRLAINAGS
                     TAENDAQQGLAHLCEHMCFNGTKNFKKSELVDYLESIGTKFGAHLNAYTSFDETVYML
                     QLPTDKEEIVTKGLQILEDWAHNVTFDSIEIDKERGVVIEEWRLGQGAQERARRQYWP
                     VLFKDSRYAVRLPIGKKEIIENFPQQVIKDFYNDWYRPDLMAIAVVGDFDLDKMVALV
                     KERFSQVPAEKNPLPLKAWNVPDNETMMVAVAKDKEMTNTTIQVIYKHNTQTPGTYDN
                     YRKGILNNLYSTMLSSRYSELSKLAEPPFLYAAGGYSSLVRAKDGFNLVTVAKPDGGA
                     KALEALLTEAERVQRFGFTPSELDRAKGEMLSDMERAYNERDKQESRALVGECVRNFL
                     EGEAMPGIEREFELTKLFMNGITLEEMNGLSKQYFTDGKNEVVVITAPDKAETKVPTE
                     EDVKSIMANVKKMDLTAYVDKVFDKPMLEKIPEPIKILAETKNEKFNITYWKLPNGAS
                     ISFKNTDFKNDEIRFQSFRFGGHSTSEDAKQRSAMVAAALINSSGLGELDANDIEKFM
                     SGKNANASPYVSELTEGINGDCAPKDMELMFQMLYQYYMAPRKDEGMFKSMMAKQQAS
                     LQNKSVSPESNFRDTVSYVMSNYHPRAKPMTAERLKEINLDDAFNFYRQRFDGVNGLH
                     FMFVGNIDEAKFRYFVERYIATLPAGNSKPEFVNLQIKAPEGNVERFIYKGTEPKSSV
                     SLRITGKMENNRKNRVGINLMMRLLSIKLRESMREDKSGVYGVGANVSTSKYPNEGYE
                     INISFGCSPDNVEMLIRAAIAEIELMKKNGASEIDITKVRETALRERESDLKENNFWL
                     SAMYNQYYFGDDINDLDSYTELVKAMDSNRYKELAIQYLKMDNFARFVLLPEKK"
     gene            74375..75004
                     /locus_tag="PK736_00330"
     CDS             74375..75004
                     /locus_tag="PK736_00330"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC31868.1"
                     /translation="MRKILLFLFILTSVSAIAQKSSNNKLVQFTGVVVTNDSLQPIPF
                     ASVMVRRTNTGTFTDYYGFFSFVARKGDTVEFACLGFKSAAFTIPDTLSENRYSIIQV
                     LLSDTISIPTITVMPWPTKEDFKQAFVNLKVNDNDITRANRNLDTETLAELSEFVAPD
                     GTINYKWAVQQQQTALYQSGQYPSNALLNPIAWAKFIQAWKNGAFKRKK"
     gene            75105..77525
                     /locus_tag="PK736_00335"
     CDS             75105..77525
                     /locus_tag="PK736_00335"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC31869.1"
                     /translation="MATVQGRVTDKNNKPIYFATVAVSGTQYGTTTDSLGNYSLTIPA
                     NEELIVAFQLINYQKQEQKVKLTVNEVITINKQLIFKPGEILTITVYADKRKNPFETK
                     INPRLPEVIAGPPSIEAGLKFSGLVFIPNELSTQYNVRGGSFDENLVYVNDVEVYRPF
                     LVRSGQQEGLSFINPDLADDVSFSAGGFNARYGDKLSSVLDVKYKKPKDFRGSFNGSL
                     LGGSFHIEGISKDTSLSYLLGVRQKSNQYLLNSLDTKGEFRPSATDVQTYIIYELNKR
                     NSISFLGNYTQNKYNVIPTDRQTEFGTVNEALRLSIYFEGQETDKFNSGTAAVTFKHT
                     VNDKLNFRLITSAFSTVESESYDILGQYFLDQLENDFGSSDFGDSAINRGIGSFLTHA
                     RNKLKAQVYNAELRTNYVTDKFLFWGGIKVQDENITDKLSEWYYLDSAGYSIPGPFDN
                     VIELNNVVKTKNNLNSNRFSAFVECQQYLDSNKRFTINYGLRSNYWTVNKQNVISPRI
                     SASYRRNRFLFKMAAGYYYQPPFYRELRDARGVLNKDLKAQRSIHFVIGSEYTFLMWG
                     REFKFVSEAYYKNLSSLVPFKTEDLRLRYFANNNSTGYAYGADFRLNGEFVPGAESWF
                     SVNYLRTFENLKDDKYYDYYNAAGEQILGGYTFDQVATDSIVKYPGNIPRPTDQRVTF
                     SILFQDYLPKFPKYKVYLNLLFGSGLPFGPPGDFRYKDIFRYPPYRRVDIGFSRTLID
                     EEENKKYKMKLLNRLRSLSISLEVFNLLQVNNTISYLWITDITNRQYAVPNYLTSRQL
                     NLRVIGRF"
     gene            complement(77831..78367)
                     /locus_tag="PK736_00340"
     CDS             complement(77831..78367)
                     /locus_tag="PK736_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31870.1"
                     /translation="MKIIICILLCTVLNTNLVQGQKTSQPDESVFRNRMTIVVDVDSV
                     KVIEMEIAAMLDNKTKPDNTKITFDLKSDWTLQFQHGFDWTELHPHQGKTINSYAIAK
                     DEIRIKGDEIFAIFEIDSEKFPLNIIPLVKARGCLYNGEWTATIINDCLIIRYVHSYP
                     DGNQTSWYREKSYYFKKY"
     gene            complement(78770..79576)
                     /locus_tag="PK736_00345"
     CDS             complement(78770..79576)
                     /locus_tag="PK736_00345"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC31871.1"
                     /translation="MSQSEELKQEVHDYWNAASCGTFAAVSEKYTKEYYDEIEQYRYT
                     KEPEIKPFAQFEKYKGKKVLEVGVGAGTDFLQWCKNGALAYGIDLTEEGINHVQHRLQ
                     LYNLSAQEFKVCDCENIQYPDNTFDLVYSWGVIHHSPNTEKAYSELVRVCKPGGEIKF
                     MVYNIDSVLSRFFWVKHALLKLKPWKSKAWVLWNHMESMGTKAYNHNSIRSMMAPYNA
                     DVISVESPFTYYDTMARHSPAMSIADKMIPLIYNKNKDGWFLKMVCRKKI"
     gene            complement(80061..80372)
                     /locus_tag="PK736_00350"
     CDS             complement(80061..80372)
                     /locus_tag="PK736_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC31872.1"
                     /translation="MKVKNRKSKTITTFDEHLDKRYGKVGTAKRTAFEIKAKAFAIGE
                     VIREERRLASMTQEQLAEKTGTKKSFISRIENGHSDIQLSTLYKLLEYGLGRKISLKI
                     F"
     gene            complement(80347..80706)
                     /locus_tag="PK736_00355"
     CDS             complement(80347..80706)
                     /locus_tag="PK736_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRC31873.1"
                     /translation="MIYFKDYFLDFFDKQIEKVKEKIDHVLYVVTVADRIPQKFFQHL
                     EGTNGLYEIRVEFQSNIYRIFCCFDEGQVVVLFNGFQKKSQKTPTEEIEKAVKIMKEY
                     FDEKLKLKQNESKKSKK"
     gene            80883..>82367
                     /gene="bshC"
                     /locus_tag="PK736_00360"
     CDS             80883..>82367
                     /gene="bshC"
                     /locus_tag="PK736_00360"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HRC31874.1"
                     /translation="MAQFLNNNFGFMGHVNTIDFSKTKILPSIVEDYFADKLLPKSLY
                     HEIPSLDAIENAIALKQLQPVNRTVLVEEFHKSYSSYGFNSKISNDERTNNPTTNNEL
                     TNNQITNHQLTSLQSENTFTITTGHQLNIFTGPLYFIYKICSCIALCKAAKVQFPQYN
                     FVPVYWMASEDHDMAEISSTTVMNEKIEWEQTWQGCSGKAPLPNFDSLIESIKVLLSN
                     KPNINELIAIFEKSYLQSQNLAEATFKLVHQLFHVYGLIIIDADNANLKALCKDIFKK
                     ELREQFSFNALSATNQQLQSTYDLQIKPREINLFYNGPESRNRIVKNGDKFEVLNTDI
                     SFTDSDAWVDANFANISPNVVLRPVYQEFILPNLAYIGGPGEVGYWLQLKSTFDAANV
                     TFPLVVLRDSFLLLDAKAQEAMTKLSLTDEDLFLTSDELSRKYIESKNINVIDFVDLQ
                     SKVGEIFESISSKIGAVDPTLVFAVGGEKQRTSKAIQAIEEKIIK"
BASE COUNT        25771 a        14803 c        15431 g        26362 t
ORIGIN      
        1 cggtctactc cacctcgatt ggctacaaac aaaaaattat aaggatcact gggagggtga
       61 tgcaatttat ttacaaatcg catcattgta tggttgctta tatattgata tatgtactta
      121 tctggattac ttaaatctgc tataccaata tcaggtgccc tccatactcc actaacatcg
      181 gagccaacat aaaagtcaaa cagtccaaga gttttttccc acaaaaaaac cgccttctct
      241 tgcccacccg ccccggggcc catttgcgcc cattgtgcat ttgccatgtt actaataatt
      301 aaaagtgcaa cgagaaagag gaagttgttt gttgttcttt gtttgatatt tctcataaaa
      361 ttattttttt taatagtttg aatatagatg cgttacaatt tattattaac aaatttgttt
      421 tgtacaacaa gaacgtaaag atttttctgc gtgtagaaat gttgtatttg ttggtgctgt
      481 aaatgtataa aggaaaatgg ttggtgcaag aaaatttttt gtataatttg taaaaagtcg
      541 aattttaata tttctgaata atattttaat ttcaaaatta attgaaatgt aattgcgttt
      601 tattaagggc gcaattgatt gaatagaaaa tatatatttg ccaaacccaa attaagagta
      661 aagtatgagt attgaaaatg aatatgccga caaaaaattt atcgatatcg ataaaatttt
      721 tttagaaaag aatgcgcgtc tgcataaaat gataccgggt tttatactta ggtatttaaa
      781 aaaaattacc cggcaagagc aagtaaatta ttttatttcg aaatacggtc acttgcgtgg
      841 gttggctttt gtagatgcca ttattaccga gtttaagttt aagtatagag taattggcga
      901 agaaaatatt ttagagcatg gctcagttat gtttgttgct aatcatcctc ttggtggtct
      961 cgatggcata tcgttattgc aagtattggg gcggcatagg cccgaagtaa agtttatagt
     1021 gaacgatata ttaaccaaac tcaaaaatct tgaagatttg tttgttggag taaataagca
     1081 tggtaatact acacgcgaaa tgctacaaga actggatcag gtatttgcat cggatggagc
     1141 attgttgatt tttccggcag ggttggtatc gcgcagaacc gatggcgtga tttgcgacct
     1201 cgaatggaag aaagctttta tagcaaaggc aagaaaatat aagaagactg tagttcccat
     1261 acatatcagc gggcatctta caaattactt ttacggctta tcgaatttcc gaaaaaaaat
     1321 aggtattaag gccaatatcg aaatgcttta tttgcccgat gaaatgtata agcaagaaca
     1381 tctcaatgtt accattacta ttggaaaagc tatagaccct gcaattttcg acaaaacgaa
     1441 aactgatgaa caatgggcgc aatggatgaa ggataaagtg tatgaacttg ctccaaaaga
     1501 aaattgatat tttggaaaat ggtgattgta aaagaaattg tttttttgaa ataaattttt
     1561 taccaaataa aaaatgaatg agcaaaaagt tatagaccct gtgccaggcg aattgatttt
     1621 ggctgaatta aatgccacaa cttttttgcg caaaaccaat aatggtaata acgaaatcta
     1681 tgtagtaaat caccgcaatg cacccaatac tttgcgcgaa attggccgat tgcgcgaagt
     1741 tacttttcgt gcagcaggag gaggcacggg cttggcatgc gacctcgatg aatacgatac
     1801 caacgaacaa ccataccagc aactgattgt gtggaatatt accgataaag atattgtggg
     1861 tggctaccgt tatattcatt gcaaaaatgc cataggtcct aatggtgaaa taatgttggc
     1921 aacaagtgaa ctgttcaact tcagcgaaaa gtttattcgc gattatatgc cttataccat
     1981 agagttgggg cgctcatttg tgcaaccaaa atatcaaccc actgccacca accgtaccgg
     2041 attattttcg ctcgataatt tgtgggatgg tcttggtgcc ataattgtgg acaatgaaga
     2101 cttaaaatat tattggggaa aagtaactat gtatcttgat tttaatcgtc aggcgcgcga
     2161 tatgatttta tattttatga attatatttt tcccgattcc gaaaaattag tgacgccaat
     2221 aataccgttg aaatatgaaa cggatatagg tgcttttgca aaggaaattg aaggcctctc
     2281 atacaaagaa gcccatgtta ttttaaacca tcatgtgagg agtttgaaag agaatattcc
     2341 acctttggta aattcctaca tgaatatatc gcccaccatg aaaacttttg gtacagcgct
     2401 taattcacat tttggtgatg tagaagaaac cggaatttta gtaacaatag ccgatattta
     2461 cgatagcaaa aaagagcgac atgttaatac atatcagaaa aagtcttagc cctgtctttg
     2521 cttcttatta gcgcacgtat gttgtctttt tgtttgtcca cattttcatc ctgaccgctg
     2581 tagaaaggca ggctgaaggc aaatgttgta cccttttgca tttcgcttgt aacaacaagc
     2641 tcgccattgt ttacatgcag aaaatcctta caaagaatca atccaagtcc agtacctttt
     2701 tcgttattag tgcccggagt agaaaaactt gttttcgaat ttattttttc gagctcaata
     2761 ggagtaagcc ctttgccatt atcggtaata gtaatccaca ctaaatcgtt ttcggtatgt
     2821 gctttaatag taatgttacc atcttcggaa gtgaactttg cagcattgga aaatatattt
     2881 cttattacca atctaatcat ttcaatatct gcatatacaa acacatcttc agctatttca
     2941 cttactgcat taataaactt gctttcgtat tgaggtttaa ggtgcagcaa aacggaattg
     3001 gcaaggtgct tcatatttac cttcgacatg cttgtttgca ttccgttcat ttggctgttt
     3061 gcccaactta aaagattgtc gagcatttcc tgcgtataaa ccaatcgctc ttttgcacca
     3121 ttaattatag taagtctttc actttccgat aagtgtgttt cgttcaatat gttaattgca
     3181 ctatgtaaag ttgacaaagg tgaacgcata tcgtgcgaaa taatagagaa taatttcgac
     3241 tttaagtggt tcatcttttc gaggttttcg ttctgctcaa gaataagttt gttttggtcg
     3301 taaatttcgt tctgctgcaa ttttactttt gtacgctcgc ggttgtagct aatttttaaa
     3361 agtgccataa tcaggaaaac gaatatgaga cagaaaaatg tactcaaaaa catatctatc
     3421 attcgcgcat tttcatcatc gtattggata acccaactgc tgtggctgga gtcaacataa
     3481 tacaaacccg acatagtaat caattgcatg gcaagaataa aaaagtattg tctgcgtgga
     3541 ctgataatca ataatgtgat gagattgagc aaaaagtata ttggcgaagg gccattgcta
     3601 ccggcaccca aaaaccagtt aagcgtaatc atggtatagc ttactactat gaaaataggc
     3661 agcacatagg tatagttatt tttatagcga gactggtaat aagtgtaaaa aagtattgct
     3721 atacagctta ctatggatat gttgagccaa aaattgagac caataataaa gtttgcaact
     3781 acaccaagcg aaaataacga accgatgagt aacgctgtga ggttaaacat ccttgtttct
     3841 atagtgaagt tgtcggagtc tccactaagc ctgttgatga gcttttcaat cgagttttta
     3901 aacataaaaa acttacgtaa atatgaaaaa ttaaatttca tcaactttgt tagattagtc
     3961 ggacttagga atagccaaca aattaacaaa gaagatttgt aaaaacaaaa tacgattggt
     4021 taaagcaggg gattcattaa aaaactctat caaatgcgat aaagcgaatc acgaaaaaat
     4081 tactgatgca aatgtaccat cctatgttta acaaactaca tttacccacc caaaatgaac
     4141 aaactttttg gcgaaaacaa taatcacgaa atgataatta aagaaaccga gtttataaaa
     4201 agtgctacca atcttgagca atgtactcca gaacctttgc cggagtatgc tttcataggt
     4261 cgaagcaatg tgggcaaatc atcgcttata aatatgctgg taaataaaaa gggcttggcc
     4321 aaaacatcga gctcaccggg aaaaacgcaa acgataaatt ttttcaaagt aaataagcaa
     4381 tggatgctgg ttgatttgcc cggatatggt tatgcaaaag tatcgcagga agagcgggcg
     4441 aaatggaaaa aaatgatttg ggattacatt gaacaacgag agtttctcat gtgtatattt
     4501 atattagttg atttgaggat tgaaccgcaa aagtcggata tggaatttgt aaacaaagta
     4561 ggggagattg gtgtgccact atgtattgtg tttaccaaag ccgataaaat acccaagaca
     4621 caaataccaa aaagtgtggc aatacataaa aaggaatatt taaaacattg ggaagagcta
     4681 ccattgatgt ttatttcctc caccgaaaca aaaagtgggc gcgaagaaat tcttgaattt
     4741 attgaaaagg ccaacgaagg atttgctaat agggatatgg gatgaagtgt ggcagaaaaa
     4801 gtgtgagcct tattaattta attcaaattg tttttaccga atctttactt ttgatagaat
     4861 gtttcgatga ttagtttttg ctaatttata aatctaattt ccatcaacaa cgattcaata
     4921 attcaattcg aaattaaagc taaaaaggct gaagcggttt ttacttcgat ttccgaaaag
     4981 taaaaatctt caatgttatc agtaataatg aaattacatt ttgccttttt tgcagcataa
     5041 tattgcattc catcttccac atcttttatt tttttatctt tcgctaattg ctttaagcaa
     5101 tcttcggggg tatcaactat ctgtattttg tttccaagca aaattatttt ttgatgtgcc
     5161 ttggtgactc cacatttttt tgtagcaaaa taaaaactaa tagctatgct taatggtgat
     5221 gtaaacaatc tatagtttgg attgtcggca aggcttagta ctttggctgc aaatggaaac
     5281 gctggatatt ccgaatttaa aacagaaata attacatttg cgtcaataaa tatttttgtc
     5341 atttcttagt tgcctttgat tcgtaaaatt ccttatgaat tgatttcttt tttttgcctg
     5401 gttttgtgta tttaatttgt ggttcggcaa gttcatctat ccatttcgca attggaaagt
     5461 cttctaacga tttgtagttt tttgctgtta ctttggtaaa taaatattcg gttaatttcg
     5521 aaacacttat gtttttcgat tcggcaaatc ttttaatctc atcaattatt tcgctgtcga
     5581 atcgcaatgt taattttgtt tccataattt aaaacttttg tacggcaata ttaattattt
     5641 tgtacgacaa attcaatttt aattcaactc caatatttct tagtttcgca aaaaataatt
     5701 tatggcaaaa tcgcaaataa acaaaccaac tattcttatt gtaaacgatg atggaattac
     5761 agcaccgggc attaatgcat tggcgcaggc catggcaacc attggcaaag tggtgatagt
     5821 agcgcccgat agtccacagt cgggtatggg gcatgctatt actattaata aaccgttgcg
     5881 attagataaa gtagacttgc ataccgatta tgaaagctgg caatgcagcg gtacaccggt
     5941 cgattgtgtg aaacttgcag tgaacaaagt attgaagaaa aaacctgact tatgtgtatc
     6001 gggaataaat catggcagca actcatccat caatgttatt tatagtggta ccatgtctgc
     6061 agctatggaa ggtgccatcg aaaatattcc ttcaataggt ttttcgctat cgaactatgc
     6121 gctggatgca aacttcgaag catcgaaatt ttatgcaaac aaaatcgctt cggctgtttt
     6181 aaaaaatggc ctgccatcga atacacttct caacgtgaat attcctaact taaccaaatc
     6241 gaaagtaaaa ggaataagaa tatgccggca agcattagca aaatggcaag aagaatttag
     6301 cgagcggctc gaccctacac atcgcaaata ttattggctc accggcaagt ttgtaaattt
     6361 cgataccggt gacgatactg atgaatgggc attggctcat ggctatgttt cggtggtgcc
     6421 ggtgcaattc gattttaccg cacatcatac tattgcacaa ctcaataaaa ttaagtggta
     6481 aaaattcctg ataatattta cattggaata gtgcttgggg cagtattgct aattgccggt
     6541 tattattttt ttctttttgt aaaccattta ttggtacaat acaatgtaat atacatatta
     6601 ccacccaaac ccaaaataca tttgatgtcg ctttttgtaa cggtaataat ttttcgaatg
     6661 ctgatgataa aatggcaaaa aacagaagtc ggccgcggag ttctttttgc catgtttgtg
     6721 gctttaatgc tttattattt ttttgaattt aggagttagg atcaataaat gtttaatcaa
     6781 gatttataaa ttcaaacatt gatatgtata aaaatgcatt tttatttcaa caagatttat
     6841 tagctcaata attagcgcaa agtagagttt ttgcaatttc cattttaata attacttttg
     6901 aaagttctaa gtttttaata aaaaaaatat ttaatatgaa aagcttattt gctatttgcc
     6961 tagtctttat gttgttcaca acttgtaaca caaaccctag tagaaagatt gtcggagaat
     7021 ggaaacaata ctacggagaa gccgatgaaa catcattcaa tactttgaat tttacctata
     7081 atattacttt gacacctgaa aatgaattaa aaatttcaga ctctgcgagc cctgaaagga
     7141 tttttttgga tatatctttc tccgaagata tactgagttt ttctacacgt tttcctggtg
     7201 ggaaatcgtc taacactgtt cattataaac ttaaattcaa tagtgaaaca aaattattgg
     7261 aaggtcatat tttatcaaat agttctcgag aacaatccaa aataagttgg aaaagagtca
     7321 aatcctccaa gtagttgctt gcttactata atcattattt tattaatata cagttgaatt
     7381 ctgcggactt ggaattacta taagcatagc aaatgtgtct aaaacaaaat agcttttttt
     7441 cagaagctgg gtttttattg ctatactaaa aataacaatt acatcttttg ctaaccaaaa
     7501 ctgaaatcaa atctcagttc ataaaaaaac agtggagccg gtgggatttg aacccacgtc
     7561 caaacgcgtc aatcaaaagc tttctacatg cttagtacat ttttggtttt cgattagctt
     7621 aggcaaacat acgggccaac cactaactta tcttctgtat ctcattggca attgaagcac
     7681 ttaccaacta tccctgcatt tgcggtgcct cagtcaaaac gcggaaggga ggcgctttct
     7741 gcgagacatt gccggttaat taacttaatt aagcagcaag agcaacatca tagttgccgt
     7801 ttgaagtttg tgaaggttga gattaaagtg cctgcccaac aaagcactgc atgcttacta
     7861 attaccgccc acgctgtcga aacctttcgg ccccatgtgt gtaatttgaa ttgcaaaggt
     7921 agtattgcat tcgggtattt gtgatgattt tttgataata atattaattt cgatttcaac
     7981 aaagatggtt attctgaatg ttgacttaaa taaattttaa taaagaaata taaaaatgat
     8041 ttcttatcat acatgtcaat cttcaaaaac taaattataa aatgccatta tgatttcgat
     8101 aatacataat cgaaaattat cttccatttt aattcctact tttggcatcc ttgaaaaaaa
     8161 gcaatgacta taggcataat aaaagaagca aaacttcatc ccgataaacg tgttgcattg
     8221 atgccttcgc agtgtgtttt acttcaaaag caatatcaca atttaaaaat tcttattgag
     8281 ccaagtaata ttagaattgt aaacgatgag gaatatgtaa aagcaaattt gactttgagt
     8341 attgatttgt cgaagtgtga tataattttt ggtataaagg aagtgccacc ggggaaactg
     8401 attgcgaata agaagtattt atttttttcg cataccataa agaagcaacc acacaacaaa
     8461 cttttattac aaaccatttt gcagaaaaat attcagctta tcgattacga atgccttgtc
     8521 gatgaaaaag ataatcgcat tataggtttc ggccggtttg caggcattgc aggggcttat
     8581 aataccatca ggttgttcgg attaaagtat gccaggttta atttaaaacc acccgaatta
     8641 tgcgaacata aagctgaact tgaaaaagaa ttgcgcaaag ccaaaatggg gaatattaaa
     8701 atacttgtaa ccggaggcgg acgggtagca aatggcgcac tcgaaatttt aggcgctatg
     8761 aaaatgcgaa aagtaacccc gtatgagtac ttgatgttta attattcgga acctgtgtac
     8821 gcacaaattc atagcagcga ttatcatgtg cataaggaaa acaaaccgtg gaacagcgaa
     8881 tatttttatg atcatcctga tgagtacaat tcgtcctttg caaagtttgc tgccaaaacc
     8941 gatttgcttt tgcattgcgc tttttgggat cctaaggcac ctgttctttt tactcttgaa
     9001 gatatggcac agaccgattt caaaattcgt gttattggag atatcacttg cgacattggt
     9061 ggcagcattc cctgcacaat tgaagccacc actattgaac aaccgtttta cgaatgggag
     9121 caatatgaaa ggcaacatgt aaaaggaatt acagaaaatt cgataagtgt tatggcagtt
     9181 gataacctcc cgtgttcgct gcctcgcgat gcatctgaag cctttggagc tgaattaatt
     9241 tcgaaagtta ttccgtcctt aatgaaagat gtggatgacg atttaattac ccgggcttca
     9301 ataaccaaag atggaaaatt gacagagcgt tataaatatt tgagtgctta tattgcttaa
     9361 aaaattattt caccacttca ccatataaat caaatgcctc tgcttttgta atttttattt
     9421 gtgcaaaatg tcctctgcgc aaataatttt ttgtggcatc tatcaacact tcattgtcaa
     9481 cttcgggtga gtcgaattcg gtgcggccaa taaagtaatt tccttcctta cggtcaatca
     9541 gcactttaaa aacctttcca attttatttt catttatagc caatgatatg ccttgttgaa
     9601 gattcattat ttcttctaca cggttttgtt ttacctcggc aggcacatca tctataaaat
     9661 tatatgcatg tgtatgttct tcgtgcgaat aagcaaacac accaagccgg tcgaatttca
     9721 tatcggccac aaacttcatt tgctctgcat gatcagcttc agtttcgcca gggtaaccgg
     9781 caattaaagt tgtacgcatg gcaataccgg gaattttatt tctaatgtcg taaatgagct
     9841 tttcggtttt ctcacgcgtt attcctctgc gcatggcttt caacatgttt gtacttccat
     9901 gctgaactgg catatcaaga taattacaaa tattgctttt ttgcttcatc acttcaatta
     9961 tttccattgg aaatgcagta gggtaggcgt agtgtaatcg tatccattct attccagcaa
    10021 cttcactcag ttgttcgagc aatgaggcta attttctttc tttataaata tcaagaccgt
    10081 agtaagttaa gtcttgagct atcagtaaca attcctttgc gcccgatgca gcaagggttt
    10141 cggcttgtgc aattattttt tcgatgggag ttgaaacatg tttcccccgc ataaccggta
    10201 tggcacaaaa agaacatgga cggtcgcatc cttcagcaat ttttaaatag gcaaaatgct
    10261 ttggagttgt aagtaagcgt tcgccaacta attcatgttt gtaatcggcc cctagtgttt
    10321 taagtagtaa aggtaaatcg tgtgtgccaa aaaatgaatc tacatccgat atttcttttt
    10381 ccaattcagg tttatacctt tcgctcaaac atcctgttac tatcagtttg tcgatttttc
    10441 ctttttgttt ttgattggca taatgtaaaa tagtatcaat gctttcttgc tttgcattat
    10501 cgataaatcc acatgtatta ataattacaa tttcgctgtt gcctcgttta gcttcatgag
    10561 ttacagcaaa atcgttcgct actaattggc ccataagcac ttcactatcg accagatttt
    10621 ttgagcagcc aagtgttaca acatttacgc tgcggggttt gtaggttttt gtacgcactc
    10681 gttttttatt ttaataggct actttaaagt cttctttctt gaaaaaaatc ttcacctctg
    10741 cagggaaaaa atcattcaaa tagttgatca tattacttac acgataaaca aagcaattgg
    10801 tgatttttgt ttcgcaataa ttgtcgtttt ttttcaaggt catatttatt accctgggat
    10861 aatggtcgta agtaatgtat cccatcgaat tgaactcatt gaaatattct ttttgtaatg
    10921 agtcgcaact aaactttgta aaaaataatt gtatttgttt tttttccgat ttcgtgagca
    10981 tacgtttaag gtaatcgact ttagtttttc cattgtccga cactccggtt ataaataaac
    11041 tgtcatgggt aattcgataa tcttgcgata gaaattgaaa cccattattg tctgaaatag
    11101 taatatcaat tttggagatt ttcttttgtg cagatgcaaa atcaaaaaat ccccaactca
    11161 atattaaaat gaaaaagtat ttagccatta attcaaaatt attatgataa tgatgtttct
    11221 ttaaataagg aatcaacaaa ttcgcgcctg tcaaatatct gcaaatcttc catcttttcg
    11281 ccaacaccaa tgtattttac aggtatttta aactcatcac ttattccaat aactacgcca
    11341 cctttggcag tgccatccag tttagtaagt gctaaagcat ttacttctgt tacagctata
    11401 aattgtcgtg cttgttctat tgcattttgc ccggtcgatg catccagtac aagtaaaact
    11461 tcgtgtggtg catcgggaat aaccttttgc attacgcgtt taattttccc aagttcattc
    11521 atcaatccaa ctttattatg cagcctgcct gcagtatcaa ttattactac atcggcattg
    11581 cgagattgtg cggcttgcat agtgtcgtat gcaaccgatg cagggtcggc acccatccct
    11641 tgcgaaataa ttggaacatc aactcgcttg ctccaaatgg taagttggtc aacagctgca
    11701 gcacgaaacg tatcggcagc acccaataat acactattgc cggcaagttt aaactgatgg
    11761 gccaatttgc caatggtagt tgttttacct acaccgttaa ccccaacaac cataattaca
    11821 tagggctttt ttgcgggctt gaaataattc tgttcgtcaa ggtctttata attggcttga
    11881 agcagatcgg aaatttcttc gcgcaataaa ttattcaact gcgatgtgcc tatatattta
    11941 tcggctgcaa cgcgtgcttg taatctttca attattttca acgtggtagt aacacccaca
    12001 tcactcgaaa caagaatttc ttcaagttgg tcgagcacat catcatccac tgtcgattta
    12061 ccggcaatag ccttggtgat tcgggtgaat acattttcac gtgttttttc aagcccctta
    12121 tcaagcgact ctttttgctc tttaccaaat agtttactga aaaatcccat gccaatattt
    12181 gttcttagtt acgttttctt taccctttgt tcttagttct tagtttcgtt tgttgtgcgg
    12241 attgttctta gtcccgacac tacggtcggg atgccgccca aagtgtcggc attcttagtt
    12301 tcgtttatta agcaatttat tctttggtct tagtttcgtt ttcgatgcac caaattcttt
    12361 gtttcgtttg ttgagagcca tgttatatgt tctgtcattc caattccaaa tattatatga
    12421 gttcttaatc tcaccgggtt aattcaattc atggctaaac catatctcat cgatttaatt
    12481 ttcgtctgca ataaactttg tgtagagttt cgaattcagt ttatagtatt ttttaatcag
    12541 agatactttt tttcattgca aaacaaattg agaattttac taaagtgatt atcaaacgaa
    12601 tttggcttca accaaaaatt tctaacttat caacaagttt attttcttgc caagcccgtt
    12661 ttcaaatatt ctatagagtg tggaaagttg aatatcgcat ttaccattct caagtcgcga
    12721 tatataactt ttcttagtgc caacttttat ggctaattgt tcttgggtca atttggcttc
    12781 taatcttgcg tccttaagcg tttcggaaat aacgaaatac tgtgctcgct cttcaaactc
    12841 atcgcgcttt ttaaccccaa ttttgccata ttgaatgtct aaaatctcat caaaattttt
    12901 tgcgcttttt atcgccttgt tcttttttag ttccttgttt ggttttattt ctttcaacat
    12961 tttgcttttc ttttaaagtg aaatattcct ttttcaattt taatgcaaat tcaatttcgg
    13021 gtttcggagt cttttgagtt ttcttttcaa atgcattgaa caacacaacc acatttcatt
    13081 tgtcgaagca acagaaaatt cgataaatat ttccgctctc ttcaactcta atttcatata
    13141 atccatccgt aactgataca tgttttaaaa acttttcgga gaccattgcc accgatttga
    13201 taacgcgaaa aacataacca attttttcct tcactttatt gctaactccg atatagaagt
    13261 ccttaaagta ttgtccgtgg aaaattattt gtcgttcctc gatcacgatt caaagataac
    13321 ttaaaagtta actaaaatta aatgttcaaa aaataaattg ttataaagtt gtatttcaaa
    13381 ctcgaaaata acaggacccc tccgcaaact aaaaactcaa cttaacgcga aaggcttccc
    13441 tccgcaaaga aagaagcctt taaatttaat ggatgatgat attatttatc tttcaaaaag
    13501 tctttgatat ggtcgttatg cacaacctct tgcttgaaag agtatgaacc cgaagggttt
    13561 tttaccatct ttattacttt gctaaactct ttgcctttac cgcttttgag cgttgccaca
    13621 actttctttg ccatgttgct ttattattaa aattgttatt atttaatttc tttatgaact
    13681 gtcatgcgtt tcattatggg attaaatttc ttaatctcca tacgctcagg gtcgttcttt
    13741 ttgtttttag tagtgatata acgtgatgtg cccggaagac cactgttttt gtgttctgta
    13801 cattcaagta tcacctgtat tctattgcct ttttttgcca ttttgtttta atattatagg
    13861 attaaagtga gcctttgcgt aaggcttttt cgattgcagc ggttgcaccc aacttatgaa
    13921 tagtgcgcaa agcggaggtg gacactttca atgttatcca ttgtccggtt tcaggaataa
    13981 agaatctctt cttctgcaaa ttgggattaa atctgcgttt ggttttgcgg ttcgagaacg
    14041 ataccttgtt tccaccaatg ctcgttttac ccgttaaatc acatatcttt gacatcttct
    14101 gtaaaatttg aggcgcaaaa gtactttatt attttgcaaa tgcaaatcca tctttttatt
    14161 tttataaagt gtttttgtta aaaaagaaga cctgtttttt gaagctaaat attttaatct
    14221 ccttttttct aacccacttt attgttttaa taaataattt ctatatttgc acccttaaat
    14281 ttaaaaacga caatgatttt aacatcagaa gcaaagaaag agatttttgc atcacatggc
    14341 ggaagcgcca caaacacagg cagtgccgag tcacaaatag cattatttac tcaacgcata
    14401 ggtcacttga ccgagcattt gaaaaagcaa cctaaagatt ttagtacaca acgttcgctt
    14461 atcaaattgg taggtaaacg cagaagtttg ctcaactatt tgcacaatac cgagattaat
    14521 cgctatcgtg ctatcatagc aaaattaaat attcgtaagt aatacatctg gtattatgat
    14581 aataatcaaa aaggcagttt tttacaaaat tgcctttttg tttttctacg ctctttgaat
    14641 tcaatattaa aattatggta ctttcagttt ttggatcaac gaatgggaat gtgtacgaat
    14701 ttacgaatag cgttaattct taaacattcg caattgattc gtaattcgtt attaaccttt
    14761 taatattaaa tccgtatcta gtataaatta aaaccaatgt taataaattg gtactgccat
    14821 ttcatctccc ggttattggt agtaaaatat ctataaacaa ataataattt tttatcagcg
    14881 gcaagggagg cggtgatgag aatagaataa aaaaaatgac agttaaacct gtaacacaca
    14941 gtttcgattt aggcaatggt aaacccataa gcatcgaaac cggaaaatta gcaaaacaag
    15001 ccgatggttc ggtagtagta aaatgtggcg actgcatgct attagccaca gtagtatcaa
    15061 acaaagatgc ccgcccgggt gtcgattttc ttccactcag tgttgattat caagaaaaat
    15121 atgccggagc aggacgcttt cccggagggt tttttaaacg tgaagcacga ttgagcgaat
    15181 acgagatatt gatttctcgc cttgttgacc gtgcattgcg ccctttgttt cccgaggatt
    15241 atcatgccga cattcaagta attattaatg ctatttcgct cgacccaaat gtaatgcccg
    15301 atgcattggc aggattggca gcttcggcag ctattatgct tagcgatatt cctttccatg
    15361 gcccaatagc agaagtgcgt atcggcagaa taggaggtga gtttgttata aacccaacac
    15421 cacaacaaat agaaactgcc gatattgata ttatagttgc cggaagtgcc gaaaacatag
    15481 taatggtgga gggcgaaatg agcgaagtgt cggaagccga tatggtggct gccatcaaac
    15541 ttggtcacga tgccataaaa gtaatgtgca atgcacaact tgaacttgtt gcaatgcgtg
    15601 gtgcactcga aaaacgcacc tacagccacg aaacaaatga cgaagattta cgcgttgcta
    15661 ttaaatccgc tacttacgat aaggcttatg ctgttgccat tatgggcatg gcagataaac
    15721 acaaaaggct cgaaatgttt aaagccattt tagaagaata caaatccacc tgcaccgaag
    15781 aggtattagc agagaagggc gaccttatca aaaaatacta tcacgatgtg gagtatgatg
    15841 ctgttcgcga tgcaatatta aatgaaagta aacgcctcga tggtcgtaaa ttaaatgaga
    15901 tacgccctat ttggagcgaa gtgaattata ttccatctgc acatggtagt gctgtattta
    15961 cgcgtggcga aacgcaatcg ttaaccactg ttacgttggg tagtaaaatg gacgagcaaa
    16021 tgatagacgg tgccatgttt aaaggtgaaa accgttttat gttgcattat aattttccac
    16081 cttacagcac aggcgaagcc cgtccattgc gtggtttggg ccgtagagaa gtagggcatg
    16141 gaaatcttgc tatgcgttcg cttaaaaaag ttttaccacc cgatggcgat aatccataca
    16201 cattgcgcat tgtaagcgat attcttgagt cgaatggttc atcgagtatg gcaacagtat
    16261 gtgcaggttc gcttgcattg atggatgcag gtgtacgcac caaagcaaca tgttcgggta
    16321 ttgcaatggg tatgataagt gatggtaaag gcaagtttaa agtattgagc gatatacttg
    16381 gcgatgaaga ccatttgggt gatatggact ttaaggttac cggaaccaaa aatggtattt
    16441 gtgcctgcca aatggatttg aaagtaaaag gtttgcctta cgaagttttg gaagaagcat
    16501 tggcacaagc ccgcgaagga cgtttgcata taattggaga aatggaaaaa actttggcac
    16561 attctaatgc agattataaa tcgcatgctc cacgtataat agaacttgta attgcacgcg
    16621 attttattgg tgctataata ggacctcagg gaaaaataat tcaagagata cagcgcgaaa
    16681 caaaaaccac tatttcgatt gaagagaaaa atgacagagg ttatgttcaa atttttgctg
    16741 ccgataaaaa tgccctcgat gctgccttgg cgcgtgtaaa tgccatagtg gcaatacccg
    16801 aagttggagg tacgtataca ggtaaggtta aaaacattca aacgtttggt gcctttgttg
    16861 aatttatgcc aggcaaagat ggattgttgc atataagcga aattagttat gcacgcttga
    16921 atagcatgga tggtgtattg aaagaaggtg acaccataca agtgaaactg atagaagtag
    16981 atgagcgcac gggtaaattc cgtttatcgg caaaggcatt gttgcccaag cccgaaggat
    17041 atgtagaacc tgcatcgaat ggctcacgcc ctgaaggacc gcgtggtcca cagcgcgacc
    17101 gccctcaagg tggtggtgga agacctcctc gtagggacta gttacttggt cattgagtta
    17161 ttggttattg gtcattaaat cattggtcat tatcgtcatt gcgaactgtc aaagtgagta
    17221 ttctcgaact gcgaagcaat ccctgatagt cattgcgaat tgtcacattg agaactcgaa
    17281 cgaagggaaa caatctctat ctgatgttgg tcatggtatg tttgttcaaa ttccaatcaa
    17341 cttcagttaa agtatagaat tttagatttt ctaaataaat attaaaaacc cggacacatt
    17401 gttcgggttt ttttatggat ttttgttgct taataattta cacttgaaac gtattatact
    17461 tctttccgat acacattcat atatggatga ttccattttg aaacattgtg ccgaggccga
    17521 tgaaatatgg catgccggag attttggaaa tatggatgta atagaacagt tacaaaaatt
    17581 aaaacctgtg cgtggtgttt ttggaaatat cgatggtgct gatattcgca aaacatttcc
    17641 gcgcgatgta cgcttcactt gcgaaggtgt tgatgtgtgg atgatacaca ttggagggta
    17701 cccaccacgt tacgaaccac atacaatcaa cataataaaa caaaatcctc ccgatatttt
    17761 tatttgtgga cactcacaca ttttaaaaat tatgcgcgac ccatcgaata aaaaaatatt
    17821 acacatcaac cccggtgctg ccggcaaaca tggttttcaa acagtacgta ctttagttaa
    17881 atttactttg catgaaagcc aagttgctga tgtggaagtg gtggaacttg gaaataaaga
    17941 tataatggtc aaaaatagtt ggtgatagtc tcctcaaacc cttgcatata aagcgtttat
    18001 aggaaaggaa ttgaaagtcg tttcaatttc ttttttttcc atatgaaaac tataaaaaaa
    18061 agtcatgttg gggatgcaac agcaccaatg tgattagatg gggtattcag aacaacaagc
    18121 aacgttttaa atgtaaaact tgcgggcttc tattcacatg gacaaacaaa gccgtaatgg
    18181 aaaccaaccg attcgtttgg tttaaaaagt gggtaatagg aagacaaaca ttacagcaaa
    18241 tccgaaaaga aagtggctat tctattagta ctctgaaaag atacttttat aaatatcttg
    18301 acaagccacc acaatggaaa gtatatcctt cagtaaaagt aaatcttatt atagatggca
    18361 cttatttcag caaggattta tgcctgatta tttatcgtga tagctcactt aaattcactc
    18421 aactttatcg gattactaat ggagaatggt atgatgaatt gaaagaagac ttggaaaatc
    18481 tccacaccct tggagtgaaa attgaaagta ttacttgcga tggacaccga gcgattttaa
    18541 aagcagtgcg cagtagttgt caagaagcaa cattacaacg atgcctggtt catattcaac
    18601 gaatgtgccg gatatggctg actacaaggc ctctgagtga tgccggaaaa gaactccgaa
    18661 caattatttc gcaactacat ttaataaaaa caaagcttga aagagattat tggattgttg
    18721 ctttagtaaa atggtacgaa aaacatcacc tctttgttaa tgagaaaagt ttccaaccaa
    18781 cttctaagcg ctattggtac aagcataaat tagtaaggcg ttcatttatg actattaaaa
    18841 atgcattgcc aaatatgttt cactatcttg ataatccacg aatacctaaa accaccaata
    18901 gtcttgaatc atttttcggc catctcaaaa atcatcttac aattcataga gggttatccg
    18961 aaagtcatcg aaaaaaattc ctgcagtggt atctccgttt taataacgaa tgagttttat
    19021 tagccataag gaagacactg ccagatttat attataaagg tgagcaattc ttgctcacct
    19081 ttttctggca ttagccttca cacattttat tgctgacaag ttgcgctccc gcagagcttg
    19141 cttcctcttc aaatgtgcgc ttgcattata taaatatttt ttgtcaattc caccaactat
    19201 ttttgagctc attaccaaat aaataaacgg gagtttaatc ttatagaacc atccccgtat
    19261 aaaaatacaa aaaaaatatc aactaaaatc aatctccgta ctatcgccaa tatttaaact
    19321 ttggctaaag cctttaagat atgcatcgct tccaataaca gaatgatgca aggtggctgt
    19381 ttcgagttgc gagtatgaac caataattga atcgcttata atagatgtac tgataatagt
    19441 atgctcgcca atcgacacat tagggccaat aatggaatcg ctaatgcggc aattttcggc
    19501 aatgcttacg ggctcaataa ctatcgtatt tggaaaacga tatttaattt ctgtattctc
    19561 tttcaggcgt tttagcatgg tagcatttgt agaaagtaaa ccaatacggc ttccgcagtc
    19621 catccagttt cctatttgaa aagtctttaa ttttacgcct tcggtaacca ttgccattat
    19681 cgcatcggtt agtgtatact catcgttggt tttgtttcct gtagtaatca tttggtcgag
    19741 gcacttcatt aaaaacgctc cttctttaat atagtaaaca ccggcaagag cgagattcga
    19801 ctttggcatt ttgggtttct ctaccaactt tgtaataatg ccggcatcgt tcaattctac
    19861 tacgccaaaa gtgcgtgggt cgtccacttt ttttactgcc agtgccgaaa tattttcgtt
    19921 tatcaccgat ttaaaatcga aatcgaaaat ggtatcgcct agtacaataa gcatttcatc
    19981 attgccatta ataaaatcgc gtgccgtcca tatggcatgt cctgttcctt cgcgtggcga
    20041 ttgcaccacg aatgatgctt tcaaatcagg atatttatct tttatatgtt cctctatttt
    20101 ttcgcccagg tagccaatca caaaaacaaa atcgtcaaag cctgcctgca tcaaatattc
    20161 tacaatgtgc gatagtattg ctttgccggc caccggcacc agcgacttag gttgtgtatg
    20221 tgtatgtggt cgaagtttgc tgccaattcc cgcaacgggt attattactt ttatcattgt
    20281 ttatttttat tggtacaaac ttaataaaag ttgaaaagga ttttaaacta tatttacttt
    20341 tcgaaaaagg gttaatgaaa actcaaatca aaaatgttag aaaaaaactt tgtgaaatta
    20401 attttggcaa accaaaataa caagaacaaa aattaaactt tcgtttgaat aacacctcca
    20461 accgaacatc aaaccaaacc agcatatgaa aaaagtaata ctcattattt cgttttgtct
    20521 catttcgttt tttaaattat ttgcatcacc gggcgatagt atttttgcta tgccttatgt
    20581 gcacaatatg tatgttactt ttccatatcc caatttttac gattcgctca tagccacaaa
    20641 tgttacggat atgtacttat cggtaaatat cgatttcaat ggggagttat ttcaaaatgt
    20701 tggcatcaaa gtaaaaggca attcatcgtt caataacccg agtcaaaaaa aatcgttcaa
    20761 ggtcgacttt aatacatttg ttccggcaca ggacatacat ggattaaaaa aattgaactt
    20821 taataactgc ttcaaagatc catcatttat gcgcgagaaa cttaccaacg actttttaat
    20881 tgcccataat attcatgcac cccgcacagc gtattgcaat gtatatttca acaatcaatt
    20941 gtggggtata tatgctgtgg ttgaagctat cgatgatgag ttttgtaaag cttggtttgg
    21001 caataatgat ggcaatttgt ttcaaggtga tccgcaagga agcttgcaat ggtttggtgc
    21061 acaaccttcc gcgtattacc aaaaatatga tttagaaaat aataatacac ttaacgactg
    21121 gaccgatttg gtaaatctta tcgatgtgat aaacaacact acaaatgcaa atttacaagc
    21181 ggccctcgat gcagtgttaa ataccaatga ttttttgaaa caatgggtat gcttaaatat
    21241 gttttcgagt ctcgattcat accttggtag tgggcacaat tattatattt atcacgatac
    21301 acttacaaat aaatttcaat ggattgcatg ggacaacaac gaggcatttg gtagttttaa
    21361 aatgcagctg tcaaatttac aattgaaaaa tttggacatg tattacgtaa gcaatgccac
    21421 ctcacgacca ttgataaata aaatgcttgc aaacccagca tataaatcat tatataatca
    21481 agcctattgc gaattgaaag acgattttac caatgcatat ttcgatagca aaatagactc
    21541 gctcgatttg caaataaaga gcagtgtgta tgccgacccg aaaaagtttt acaccaacag
    21601 tatgtacgat agcagcctca catatgattt gaacataaca ggtccacaag gcataaatgt
    21661 ttttggatta aaaaactttg tttctacacg cgcattaaat attcaaaata atttcattgc
    21721 taataatgtt gtttgcacta actcaattga aaatagtgca gataatttca atctaataat
    21781 atcaccaaac ccggttaaaa acagtattca tattatttct tcgtcacttt gttccattta
    21841 taaattatac tctatggatg gaaaagaaat agccagtggg ccatgcaatg gagatattga
    21901 tgtgagtaat tactctgcag gggtttattt tttatctgtt ttaattaatg atagattata
    21961 ctatcaactt gtgaataaaa ttgattagct actttcatgc aatgttttat cttcatattt
    22021 gtttgctttt tttaggattg tgaattgact ttaatatttt tgcaaaagga aaaaattcaa
    22081 ctcgatttta aaaagataaa acataagtaa ctctttatta aattatgaaa ggtactcacc
    22141 ttaatgcact taacagaagt atttttgtat cattattttt attgctaaca tcaacaatca
    22201 gcttgttttc gcaaacagaa aaagcagcta atcccccaat ttatattgcc tttttatggc
    22261 atatgcatca acctatttat tggccatacg aaaatgtggt cacaacccac aacaccaata
    22321 aatacccttt taacttgttg cagattttca atgatagaaa tggaccatat acaaactggc
    22381 ctaaggatgc agtgctgcaa ggggtaaaca atggaatgcc tcatttcggt gcgcaagtaa
    22441 gtttttcggg ttctcttatc gagaacctca ataacattag cgctgcaggc cttggcttca
    22501 ataattggaa atcgaattgg aacacgataa aaaatcaaac aacttcgctc ggaaatcccc
    22561 gtatcgatat ggttggcttt ggctattttc acccgctgat gggcttgact aactatgccg
    22621 atatacgcag gcaaatacaa gcacacaaaa ctatgatgag tgcaaacttt accggcaact
    22681 attctaaagg aatattccct cccgaaaatg ccttttcaac agaaatgatt cctgctttga
    22741 aagatgaagg cttagaatgg gtaatggtag acaacataca tttcgacagg gcatgcaatg
    22801 gatatccttt cagtacaaat ggtaaccttt acgaacccaa caaggctgac ttacgaaatg
    22861 ctgaccctgc cgattgggtg gcactcaatg gcatatgggc acctacacag aatagtgctt
    22921 tatggggccg tcaacctcac tatgtttcgc acactgaccc caataatgga actgtgtcga
    22981 aaataatagc cgtacctacc gatcgatatc ttggcaatca ggatggtcag ggaggttttg
    23041 gagccttaag ttatgattat gtaatgtcgc agcttgaacc attcaacact aatagcaatc
    23101 atccaatgct tattgtgctg catcacgatg gtgataatta cggtggagga tcacctgcat
    23161 attaccaaaa taattttgcc gcctttgtta gttggcttca ggcaaatcca acacgttttg
    23221 tttgtaccac tgtgcaagat tatcttgata tgtttcctcc tgatacaaat gatattattc
    23281 atgtggagaa gggatcgtgg agcggtgcgg ataatggtga cccggaattt aaaaaatgga
    23341 atgcagatta taacaattgc gttagccccg accgaaacag ttgggcggct ataactgctg
    23401 cacaaaatta tgttgcaaca gcacaacaaa ttgatagcac ccaacccgga acagtatcgg
    23461 catataattg gctcatgtgt ggcgaggcca gtgattattg gtattgggat aactctttgg
    23521 gcggtatatg ggacagtcat ccaaccagag ctgcaaacca agctatcact ctttcacaac
    23581 ccgttcttca attgggaacc gatataacag gcccttctat ttggtcacca cagcgtgagc
    23641 cttacaatcc gggtaaaaca gaatggaacc aaatgcaatc aagtgatttt acaatttgga
    23701 cctatgcttt tgatgtaaat gggattcaaa acatatcgtt agaatggcga accgatatag
    23761 acaatgtact ctcctacgaa aacgatattt acacaggtgg aattggcgtt aatgcatgga
    23821 acacaaatgc aatgaatgct tcatatattc aacctcaaac aattatttca cctatagcaa
    23881 aagctaaaga atattcggca aaaatatcag gacaaggaaa tactatgata gattattata
    23941 ttaaatgtac cgataacttt ggaaacataa gtaagagtga aatacagcat gtatgggtag
    24001 gcccggccaa taccatcaat catacatggc aaaattgcga tacaagtgtg gtgcaatcaa
    24061 atcctctgcc accgggcgtt tcgtggcagc cggcatttcc caccacccag gatactatca
    24121 ccgtatacgt tggcggggca aatcaaggag ctaatttaca ctggggagtt aaccaatggc
    24181 agcaagtgaa taacagctat tggccggcag gttctactct ttttaataat gttggacctg
    24241 ctatagaatc gccaatgagt gcccccgaca attgggggaa tattcaaatc aaaatcgggc
    24301 ctttcaacaa acctgttcag agtgtgaaca aagtggactt tgttattcac tatagcaaca
    24361 acacatggga caataacaat ggaaatgatt atcacattcc attaatgttt gtaaataatt
    24421 tcgatcattt gcccgataat ggattaaatt gtacaataaa ttacaacatt aatggaagtg
    24481 ttttaaattt aggcataaat tctcaaaaat atgagaagct aacagtttgt atatacgatg
    24541 taaatggaaa ttgcatagtt gatagcacac catatgaaat aaaatcggga cacactgaac
    24601 ttggtatcaa aaccaatgga ttggctgaag gcatttattt cctcaaattg aaatcgaatt
    24661 cagttgattt tgctaaaaag tttatagtaa ttcgtaacta aaaactttat tgaatattcc
    24721 tcaagcgttt atttgccgtt ttgagttttg gaaatttata gaacaatatt aattaccagc
    24781 tattttttgc acatatccca ttgtgaaatt tattgcttgg ttctgcaaat caaatacggt
    24841 tatcttattg tcatttcaaa tatgtgattt ttaaatttaa tgaatgctca atcaaaattc
    24901 gaaaaagcca tttttgcttg gagggcactt cggcctttaa aattaaaaat taaaaaaacg
    24961 aacagatgta atccaatcat cataagttac taattgtata aaaattgaaa gcaacaattt
    25021 taaataccat cagttaatgc aaattatgaa ttaattattt tgattgaaaa gtcatacttt
    25081 ttctggttta cttgcatgtt tcactgttat ctttttttgg tctaataaag caattacaat
    25141 aatttctctt tgcaattaaa ttgttttatt tattattgcc ctatataagg ctatgataga
    25201 aaaagacacc gcacacacaa ttgctgaaca attgttgcaa ataaaagcaa taaagcttca
    25261 gccgtctgaa cccttcactt gggcatcggg ctggaaatcg cctatttact gcgacaatcg
    25321 taaatcactg ggctatccca aaatacgaac ttttattagg caatcgttgg caaaacttat
    25381 tgaagaacga ttcggcaagc ccgatttaat agcgggcgtt gccactgctg ccatagcacc
    25441 cggagtttta gtagccgaag cattgggttt gccattttgt tatgtgcgca gtactcccaa
    25501 agatcatggc ttgaaaaatc aaatagaagg cgattacgca gcaaaccaat cgtgtgttgt
    25561 aatcgaagat ttaattagca ctggcaaaag tagtttggca acggtggagg cattgcgtaa
    25621 agaaaaaata atagtgaaag gaatggtttc tattttcagc tatggttttg aagatgcagt
    25681 caataacttt aaaaatgcca actgcgtttt atattcattg tgcaattacg aagtattact
    25741 cgaagaagct cttgccaaga aatacataaa gcccgaacaa ctgagtactt tacgtgactg
    25801 gagaatttct ccttcgacat ggatgggggc ttaggttttg gaatttagac ttttagtatt
    25861 tagtattgag acttttagta ttgagattat tagtattgtg tattgggatt tttggtattg
    25921 agatttttag attttagtat tgagaccttt agtattgaga ttattagtat tgagtattgg
    25981 gatttttggt attgagattt ttagatttta gtattgagac atttggtatt gagattatta
    26041 gtattgagta ttgggatttt tggtattgag atttttagat tttagtattg agacctttgg
    26101 tattgaaatt aaattgatga ttgttatttt gcttgaaaag agacgggatt gaaattatga
    26161 tgtgtaaaat tttaagcatg aaaattaatc actgatagct aaactaaaaa ccctacttat
    26221 caaaagccaa aataaaaact aaaaactgca tttaaatgct cgcatcccga ccgaagtgtc
    26281 gggactataa actgcattca aatgcttgca tcccgaccga agtgtcggga ctaaaaacta
    26341 aaaacagcat tcaaatgctt gcatcccgac cgaagtgtcg ggactaaaaa ctaaaaacta
    26401 aaaacagcat tcaaatgctt gcatcccgac cgaagtgttt ggactaaaaa ctaaaaacta
    26461 taaactgcat tcaaacagca ttcaaaaaat gacatcaata aagagtgaaa aagtattaat
    26521 caatgtgcct gttcaaaggg tgtttgagtt tttgtgcaat ttcaataatt accaacagct
    26581 aatgcccgag caggttaccg attggcaaag tgatgatgat agtggtactt ttaccattaa
    26641 gaatatggca acaattgggc ttcgaattat cgataaaata ccattcgaaa aagtacacct
    26701 cgaaaaaacc aaagcaccat tcgaacttac actcgattgt ttaatgaacg atatgggcga
    26761 taatttttct actcaactcg aattgcaact cgatgcagaa ttaaatgcca tgctaaaaat
    26821 gatggcatcc aaaccgctta caaattttgt aaatatttta gcaaccaact gcaagaaagt
    26881 gcttgaagga atttgatttg tgtaaaaaca tatcttgatt actaatcagt ggtttcgata
    26941 gttttccgac atttacacca cgaattgttt catttttcga tgcattgtgt ccaccagggc
    27001 atttgtgttt aacctaattc tccaaaagat tttttcatcc accgggttca aaaaaaaagg
    27061 ccggaacatg tccggcctta atttaatttt aggatgattg attatttccc agcctttgct
    27121 gtagcacggg caatatattt ttctacatcg cgggcttctg tactttgagc aaagttggtt
    27181 tgtatttttg tataagctgt tattgctgaa gtataatcac ctttaagttc ataggcaaat
    27241 gcttgttttt tcaaaaagta gggcgcagta aattgattat cgtcccaatc tgccgctttt
    27301 ttataaaaat caagagcatc gtcttttttt ccaagttcca tgttggcatc gccaattgca
    27361 ccaagagcaa gcgcaccggt aatatcatct tcggcatcat aatctttaag ggcatcaata
    27421 gcattttgcc attggtcttt tttcatatag cacattccaa ggtaatattt tgcaaggtta
    27481 ccggttttgg ttgaaccata ttcgtctata atctgtttga agcccatatc ggcacctttg
    27541 ccattaatag ccaaatcaat agaatcgttt ttgaaattga actgagccgc aaaaattgac
    27601 tgctgagctt ttaactcttt cggcttcaac caaaagttgg tataaagcac ataagccgca
    27661 agtacagcca ctattgcacc accaataatt attaagcttt ttttgttgtt ttcgatatat
    27721 ccttcaaatt tattgatgct tgcaccaatg tcaagggtag tctcttttga atctgccatt
    27781 tttagtttta tttttttctt aaaaaatttt agggtgcgaa tttaaaaaaa gtattttagc
    27841 tgcaattatt taatctaaat gttttttaac gttttttgta cattctatgg tcaataatca
    27901 ttgcagcatg atgcaaataa gtatttgttt tgataaattt tcattttaat ctattcgttt
    27961 tgtatgaaaa aagccacagt ttcggccaaa tctgttaata aaagcaaagc tacggcagcc
    28021 caaaggaata aaccttcaat accaaaaggg atttcctggc attggtggct tggcctggca
    28081 attgttgttg tttctacttt tgtggcctat tccacttgcc tcgataatga ttttacaaac
    28141 tgggacgacc cgggttatat tattgaaaat ccaataataa agacaatgtc gggcgaaaac
    28201 atcaagcgta tttttaccga gccatacttt gccaattatc agcctttaca cattttctcg
    28261 tatatgattg aatatcactt ttttgggctc gatgcctcgg gatatcattg ggtaagtgta
    28321 ataatgcata taattaacac tttgcttgta gcctttattg ctctactgct gctcaaagac
    28381 aggtatattg ccattgctgt ttcgctttta tttggagtgc acagcattca tgttgagtct
    28441 gtggcttggg cagcagagcg taaagatttg ttatacacca tgttcttttt tacctcgttt
    28501 attttttata ttctttttgt gcaaaaatcg caaaccaagt ggcttatagt gtcggttgtg
    28561 ttttttgtat tttcaatttt ctcaaaagcc atggccgtgt cattggtgcc ggttttattt
    28621 cttatcgatg tactttattc gcgcaaacta tcggttaagt tgtttctcga aaaaattcct
    28681 tatctcattc ttgcgctcat attgggtata gtttcgctca aagtaaccaa acctgaggat
    28741 gcttttactc ccaccggtga tacttacact ttagtgcaac gcttgttttt tgcttcacat
    28801 aatttgttgc aatattttgc taagtctgtc tttccatacg gtcaaagcgg gtattaccaa
    28861 tatcccattg tgggcaatca aacattgcct gtgcaatatt acatcgcacc aataattgtt
    28921 ctggcttttt tgggtttgat aatttattcg tggcgtaaaa cacgatggat agttgtgttg
    28981 gcaggattct ttacggcaac cactttcctc atattaatgc tgtataccat aggccctact
    29041 atattttcgg aacgatatac atatatatcg tctttggcgg tttatttatt tataggactg
    29101 ggtgttcaat attttgcttc gggtaatggg ccttttcaaa tgagtaagaa tattggatta
    29161 gggttaatag ctgtgtacag tgtttggtta attttcaata cacgcgaacg gtgcaatgta
    29221 tggcaagata gtatcacttt ttggaacgat gttatttcta aagactcgcg tattcctatt
    29281 gcatataaca acaggggcaa tgagtataaa agtcgcaatg agtttgacaa ggctattccc
    29341 gatctgaacc aggctattgc ttataaagaa gattaccttg agcctcatgt catcttaggc
    29401 gatatttatc gctctcaggg aaagtatgaa ttggcacgcg aaaaacttga aaaagcattg
    29461 aaaataaatg ccaaatcgca ccaggcatta gtaaatcttg gaatagtaaa ttgtgtgcag
    29521 ggtaggctag aggatggatt cgataatttt aataaagcca tttcttataa gcccgaaatg
    29581 tttgaggcat atggaaaccg tggtaattat tatgccatga aaggtgaaat tgataaagca
    29641 attgtggatt ataacagggc tttgcaaatc aaccccgatt ttaaagatgc ttatctcaat
    29701 ctgggaaaaa cacaaatgga acaaaagaaa cctcaggaag cactaaagta tcttgattta
    29761 tttattcaac gtggcggttc tcccggtgaa gcaaactggc ttaaaggagt tgtgtatgca
    29821 tcaatgaata attttaatgc tgctttgcaa agtgctaatc tggctcaagc tgcagggtat
    29881 gcggtgaacc aacaacaaat gcaattatgg gcagcaggaa aacaacagta accgtatacg
    29941 taccatcaaa aagaaattcc aaattatttt atttcctggg cctccaagca ttgctcgtat
    30001 agtctttcat acatgggtct gattttgtcg atatcgaaag tggcagcatg cgcaagggcg
    30061 ttttttttga attgcagaag cttttcttca ttcaacaata aatcaacgga atttttggcc
    30121 atgtcgtcaa tatcgccaac attactaagg taaccggtta ctccttgcac attcagttct
    30181 ggcatgcctc ccgaattggt tgatatcacc ggtacctgac aagccatagc ttcaagtgct
    30241 gccaatccaa agctttcagt ttcgctgggc ataaaaaaca aatcgcacac cgaaagaatt
    30301 tcttcaatca tttcttgctt tcccaaaaag cgtacatcgt cacatacctt caaatcgcgg
    30361 caaagttgct cacaataaac acgttcgggc ccatcaccta tcatgagcaa ttttgcaggc
    30421 acttttttta ttattttttc gaatgcataa attacatctt gtgtgcgctt taccttgcgg
    30481 aagtttgaag tatgaacaat tattttttct cccgcgggtg ctattgcttt tttgaaatga
    30541 tctttacgct gcttgttgaa gcgcgacaag tcaataaagt tgggaacaac atcaatatgc
    30601 ttggtgattt tgaaatttaa aaatgtttct tctttcaaac tttcagatac tgttgtaact
    30661 atatccgaat gatttattgc aaaggttacc acaggcgcgt aacttggatt ttttcccacc
    30721 aaagtgatat cggtaccatg caacgtagtc ataaagggta catgaaatcc ttccgatgca
    30781 aggatttgtt ttgcaaggta tgctgccgat gcgtgaggaa tggcgtagtg tacgtggaat
    30841 aaatcgagct tttcatactt tgccacatca accattttgc ttgtcaatgc attttcgtat
    30901 ggcacgaatt cgaacaaggg ataatcggga atggtgactt catgatagga aatattttca
    30961 tgaaaatgat ctaagcgcac cggctgatta taagttataa aatgaacttt atgtccattc
    31021 attgccaaag cttttcccaa ttcagtggct attactccgc taccaccaaa tgttgggtaa
    31081 cacactattc caattttcat ttattttttt ttacaatcaa caaattaact cattgttttg
    31141 ttattcccac aatacgtatt tgtgaattta ttatttgccg gtgatattgg ctttttagtt
    31201 atttgatatt ttgaaattcc acaactaaat gttcgcaatt tcggtccacg gaagaacact
    31261 caatttgtca ttcatcattt tatgtgttgt acctccatgt atcaacatac agtggctttt
    31321 gggctggtat ttttgccaat aatttaatcc acgcaccata ctgttgtcaa actttttact
    31381 tgcttttatt tcgattgctg ttgtttctcc atctcgttcc ataatcagat ccacttcgtt
    31441 tcctgtgctg tctctgaaaa agtacatgcc atcatttaac ccgctgttaa aattattttt
    31501 acgaagctcc gaaataatcc aattttcgaa aatggcacca taggcattat tttgctgcaa
    31561 tgctgttaca ctttttatcc ccagtaaatg gcataataaa cctgtatcgt aaaaaaacaa
    31621 ttttggactt tttataattc gcttattcaa attgttgtac catggttgta gttggaaaat
    31681 gatgtaactg ttttctaata atccgagcca ggcaagaatg gtctttgtat ccacacccgt
    31741 ttgctttgcc aactcatcgc ggttaagtat ttgccctgca taatttgcac acaaataaac
    31801 gaaccgattg aaagcggcca tgttggtaat gttgcgcagc aatcttacat cgcgttgaat
    31861 gtatgtttgc atataactgt taagccaaat tccaggcgac aatttttggt tccagatttc
    31921 tggatagcct ccggttaaga tgtttttatt tgcttcgctt gttgcatatt ttgttttcga
    31981 taactcggca taactcaagg gtaataatga taaatacccc gctcgcccgg ctagcgattg
    32041 tgaaacttgc tcttgcaaca aaaaattatt tgaaccggta agaataaatt gcccgcgttt
    32101 attattattg tcgagcacac cttgcagata tctgaaaatg tgaggcacac gctgcacttc
    32161 gtcaaacact gcaccgttgc ggtatttatg taaaaaggcc cgtgggcttt catcggcaag
    32221 tttttgcaca tcaggatttt cgaaatttac atacggctta ccggcaaata attttttgct
    32281 caatgtggtt ttaccgcttt gcctggggcc ggtaatgcaa accgctctaa attgacgaag
    32341 cgtttctttt gcggtaggaa ggatcaatct gcttatcatt gcaattccat ttttgcaaat
    32401 atatgtaatt atggaatcca attccatttt tgcaaaaaaa atacttttaa taccattttg
    32461 tttcaattcc atatttgcaa aaaaaactgt aaatatggaa tccaatctta ttggcgtaaa
    32521 aaaacaatgt tggtttatcg aaattccata tttgcaaaaa actgtaaata tggaatccaa
    32581 ttccattttt gcaaaaatgt gcaaatatgg aattggtaaa aatattttat actttatttg
    32641 agttgaattt acgcacagtt attttttagc acaatgcaat tttcattaac cctaaaaaac
    32701 attggcgtct acctaaaata aattaaaatc gttgaaagtc ccgtagggac gatattttgg
    32761 tagaaacaag aataaaaaaa agaatcccta aggatgatat attaagcgtg aaaaaatacc
    32821 gcaacaaaaa tttcgttcct acggaacttt gatgcaggtg gtttttcgtt gttaccaaaa
    32881 tttcgtaagc caagccgcgc cagccatggt ttgtgtcccc acaaaccatc atcgccatgc
    32941 cgttgctctg ccactgtttg tgtctacaaa aaccgtcacc accattccgt tactcttttg
    33001 tgtattatcc gggttttcaa ccactttcaa taggatgcca tcacaatgaa gcacacgttt
    33061 aaacccgttg ctacaatatg cttcgcccaa ctggacttta aaaagaaata atttaaatta
    33121 tgcacaatcc ttaaagggtt caacatagtc ggcattgaaa tgtagaactc catcttccga
    33181 attgttgaca agaaaattta tgccgttctt agtttaaaat aaatttggct ttgcaaacat
    33241 gagaaatatt caaaaaatct attgcgctga ataattcttg tcaatcgttt tgagagcata
    33301 gcaaaattat tttgcagctt cggcatagtt catataaaag tgtggtatag tttctatccc
    33361 tttcaagaaa ttgaaaactc cgaaatgttc gtttggagaa tgtatcaaat cgctgtcgag
    33421 gccaaatccc atcagtaccg attttaatcc taattcgcgt tcgaataaag caacaatagg
    33481 aatactgcca ccgccacgtg ttggtatagg tcttataccg aatgtggttt ccatggcttt
    33541 ggcggctgcg ttataagcaa cactatccgt agggcacacg taaggctcac ctccatggtg
    33601 ggggtgaact tttactgtta cacaatctgg tgcaacggct tcaaaatatt ttgtgaacaa
    33661 ttctgtaatc ttatccgact tttgattggg caccaaccgc atcgaaattt ttgcaaatgc
    33721 ttttgaaggc aatactgttt tcgaaccttc gccaatatag ccaccccata tgccattcag
    33781 ttcaaaagtg ggacgtatac cggtgcgctc aattgtggta aatccttttt cgcctaaaac
    33841 atcttttaca cctaaatctg ttttgtattc ttctaaatcg aaaggtgttt tattaagttc
    33901 tgtacgttca gcatcattca gttccaacac atcatcataa aatcccggaa tggttatgtg
    33961 gttgttttca tctttcatgt ctgttatcat tttacacaga atctgaatag gatttcctac
    34021 ggcacctcca tacactcctg aatgtaaatc gcgattgggg cctgtaactt ccacttctat
    34081 ataacttaaa ccccgtaagc caacatcaat acttggaata tcgtttgcaa ccaagccagt
    34141 atcggaaata agaataacat cggcttttaa tttttcttta ttgtttttaa caaatgttcc
    34201 aagatttgcc gaaccacatt cttcttcgcc ttcaatcata aattttatat tgcaaggcag
    34261 ttggttcatt tgcatcatca tttcgaatgc tttcacatgc atgtatactt gtcctttatc
    34321 gtcacaagct ccacgggcaa atatctttcc atcacgtatt tgtggttcgt agggtggcga
    34381 ttcccacaat tcaacagggt cggcaggctg cacatcgtag tggccgtata ccaaaacggt
    34441 ttttaatttg ggatcgataa ttttttcgcc atatacaatg ggatgccctt ctgtagggca
    34501 aatttcaact ttatcggcac cggcaatggt aaatttttcg gcaataaatt ttgctgcacg
    34561 tgccacatca tctttgtatt tactatcggc acttaccgat ggaatgcgca accattccat
    34621 taattcactt aaaaatcttt gttcgttttt gttaatatat tctttaatat tttccataat
    34681 aaaaattaat tttgtgagcc aaatgtatgt aataagatta atgcacaatg aacacaggtg
    34741 aaaataattg gtaatctgtt atgcttaatt tctatactac tattttagca aaataaatct
    34801 tgtaaaaatt ctgtcttgaa aaaaataaaa caacaattaa taaaaagcgt ctgttgcttt
    34861 ttcggtttaa ttctcattca tgtttcggca atagctcaac ctataaatga tgagtgttat
    34921 ctcgcaaccc caatagttgt tccccaaagc gggcaggtat gcattaatgc atcaaatgca
    34981 aacgccaccc ccgatgctat cactcctaat acttgcaatc agcctacagg aggtaacgaa
    35041 gtttggttca cttatatcac aaccggcacc aacaatctaa ttgctgtttc gccaagcgga
    35101 ggaacgcctg ctcaaacact ggcattaact atacctacag gcggatgtgc aggaaatata
    35161 ccattggctt gcaatgcatc ggcaacctta aatggtactg ccattattaa tcaatcgttt
    35221 cctgttggca ctcaggtgtg gttcgaggtt acagctttaa catcggatgg tgattttaca
    35281 atttgcatta catctgccga taatccacca gcatatgcgc ctaatgtagg cgattcctgt
    35341 acaaacgcta ttcaactttg taacaaacaa tcttttacaa cacttggtgc cccgggcttt
    35401 gccaactcag gtgcattttt aacttgttac ggttttggag ctttgaatga tatttattac
    35461 aaattcgatg tcggaaaaac tggtacactc gaatggctgg cgaccccaag cggagctata
    35521 aacctcgatt gggaagtact tgatattact ggcggttgcc cgggagtttc tattgcatgc
    35581 aattatactt atgttccacc cggtggacaa tcgaccggaa tgcaagatac atctacaact
    35641 ccttgcggaa atattggaca catctgtcaa tcggcaattg ttaccgcagg caatacttat
    35701 gtaatacact tgaataatac ttcctttttg ccgggagtag gtttcactat aagttttggc
    35761 ggtactttcg aaatggcacc ttttccaagt tattccttaa caaactctat agggtgtggc
    35821 acacttacta ccagtattgt tgatagtagt gttgggtcaa caaattacaa ttgggtcttt
    35881 ggtaatggaa acacttttgc aggcgccaat cctcccgcac aaacttacat agcaccgggc
    35941 acttatatga cttcattagt ggtgagcaat gcatcatcgg gttgttctca tgttacatca
    36001 gtgccggtag ttgtgaaaga aaagcccacg gccgatttta ttttgacaga cactaccatt
    36061 tgtatagggc aatcgaccag tgcggtatat gttggtacag ctacaccggg cgcaacttat
    36121 acctggaatt ttagcggggc aaacgtaact tcgggaacag ggcaaggacc atatacttta
    36181 gactggccaa ctgtgggcag taaaactatc accttatacg ttactgaaaa tggatgcacc
    36241 agcgatacca ttacaaagac agttgttgtt agtgccccgc ctgttgcaag tttttcttta
    36301 cctgccactg cctgcacagg tgatactttg ttggtgacgt atactggaac cgttattccg
    36361 ggtgcaactt ttttctggaa ttttggcaac ggtttttcgg caggaggaaa tggaaatgat
    36421 actgttttga ttacatggcc aactgctcaa acagatagta tctcgctcac cgttagtgca
    36481 ggcggatgtc tgtctacaat tctatatcat accattgtaa tttccgacaa accaacatca
    36541 acatttactt tgcctgatag cacttgcaca aacaacacca ttacacttaa ctatatcgga
    36601 actggaacgg ttgcagctgt ttacaattgg aattttgccg gaggtaatgc tttgcccggt
    36661 ggcacagttc aaggtccaca taatattaca tgggtaggta gcgggcttaa acaaattaca
    36721 ctttctgtta cagaaaacgg atgtacttca tctattacca aagacacggt atatgtaatg
    36781 cccggacccg atgcttcatt ctcattaagc gatgatactt tatgtacagg agaaactgtt
    36841 acggtaactt atacaggaac tactccggga gcaattttta attggaattt ttcgggcgca
    36901 aacattttga gtggaagcgg agcagggcca tatgtgcttg attggccaac tacaggtgtg
    36961 catgttgttt cgctgcttat gagttttaat tcctgcaccg actctattgc cgatactata
    37021 tattcaggtt taactccttt tgcttttgtt ggaatagaca aaacatattg ctcgggcgat
    37081 tcggttacaa taggtgctcc tgctgtgcca ggtattactt ataattggtc gccagctttt
    37141 ggattaagtt ctgctactgt ttctcagcct accgttaacc ttactaatac aggcggagcg
    37201 gctatagttc aaaattatat tttaaccaca agcaatttat tatgtacggc taatgatact
    37261 gttcaaattt cagtaacagc aaaccaacct gccaatatta gccttgtagg aaacaacttt
    37321 acccaatgta ttgccaacaa cgattttgat ttttcgctat ccggtccact tgtaccagga
    37381 gcaagcatca attggacctt tggcgctgca gcatctccac ttaccagcac taatgcaata
    37441 gtcaataatg ttacatataa tgccgtagga acttacattg ttcaattaat cacttcggca
    37501 aatgggtgcc ctgccgatac ggatacggtt acagttacaa tacaagatgc acccaaggtg
    37561 aaatttgggg ccgatacttt gataggatgc ccacccttgt ctgtatcgtt taccgatagt
    37621 tcaacagcgc cacccggctc cacttatgtt tgggattttg gcaatagcga tacatcaact
    37681 ttggaaaatc caaactatac ttatacagca tcgggtttat ataatgttac acttactgtt
    37741 acctcaccca acggatgttc ggcatcgagt gttgtgaata attacattaa tgtaacgcaa
    37801 gaacccactg ctgtgctcga agctgatcca aatattacaa caatactaca gcccaatatt
    37861 caatttttaa atgtgtcacc ttatgccgat tcatgctata tcgattttgg tgatggtact
    37921 tttttcaatg gatgcggaaa tattgaacat atttatggcg atactggtac ctatatagtt
    37981 acattgtaca caatgagtgc cggtgggtgt gtcgattcaa cagaaattac tgttatcata
    38041 aaaccttttt tcactttatt tattccgaat tcgtttacac caaacaaaga tgatttgaat
    38101 gaattgttaa aagtttatag cgagggagtg aaagaatttt cgattggcat tttcaatagg
    38161 ttagggcaag aagtttatgg cacattgaac aaagattttg cctggaatgg ttatgatacc
    38221 aatggaaagc ccttgcccga aggcaattat gtgtacataa taagagtgaa ggacgttata
    38281 ggaaaacggc actacaaaca aggtatagta agtatagtac gttaacatcc tttaaatctt
    38341 agttaatttt tcaggagaaa agggtataat aattgcttta tatcagaaaa tagaatttat
    38401 ttgtataaat aatttgaaag ttcacacaat aagtaaaaat taacttcgtt taaaactttc
    38461 ataactacca aatttttaaa aacaaagctg ccgatagata gaatttacca ttagtatttt
    38521 tttagtttaa acattttaaa ccaataatta atgggaagta gtatgcagtt taatccgctt
    38581 acagacaacg aacttattca acagtacctc aaaggtagtg atgatgcatt gcgcaacttg
    38641 attgaaagac acaagcgcaa gattttttcc accatttatc ttttggtgaa aaaccgggac
    38701 cttgccgatg acctttttca ggataccttt atcaaggtga tcaacacttt aaggggtggc
    38761 aattaccagg aagaagggaa atttttgccc tgggttttgc gaatagcgca taatctggtg
    38821 atagactatt tccgcaagca aaagcgtatg caaatggtac acgatactga agagtacagt
    38881 attatggaca cgcttcgact aaccgatgac aatgtggagg acaaaatggt gcaagaccaa
    38941 attcttaatc aggttaaact gctgattgat gaattgcccg aagaccaacg cgatgtggtg
    39001 ataatgcggc attacaaaga catgagtttt aaggaaattg ccgaaacaac caatgtgagt
    39061 atcaatactg cattgggccg tatgcggtat gcactgatta atctgcgaaa aattatagaa
    39121 gataaaaaac tgatgctgga gaaataagca ctcagaatat aagaaacata gcaagaatag
    39181 tttgcgcaag aatgcccgaa atttatttcg ggtttttttt tgatgcttta caatatgcca
    39241 aaaagattgt cgttagaatg acataacctt ttaaaaatgc atatgagcaa aatttctacc
    39301 acagacaatc ttatccttta ctattttaac gaaactgcaa tggccgaaac cgtattggtt
    39361 caaaatgcta ttgacattga cgttgttgta cgcgatgaat acgaagagat aaaagccgca
    39421 ttcgatattc ttgacgaatg cattgccaca cccaaacaat caagtataga ggctatcatg
    39481 agctattcga agctgactca gtgccttagc tgatttttgt cttttgggat tttttatacc
    39541 ataaaattgt taggtgaaat cttgcttgcc actttaaggt tgcaaaaaaa atcaaaaaca
    39601 gtgaaccttc tttaggtttg ggctttatat aaattagccg atttttaacc cgcttctttt
    39661 tttccccttc tatcaaaaaa aagtatgctt ttgtcatttc tacttttgta tctttgggta
    39721 aatcctaaat gcctaagatt ataaaaaaaa taaaattttt atttggtatt ttatttttgt
    39781 gtcaagcaag ttctgcattg cttgcgcaag ggctaaacca taactggctg cttggttatc
    39841 ttgatgcacc atatttgcct tacagtccat cggccgatgc caccttacag ttcaccaaaa
    39901 caagttattc tattactggt acatcacgcc ttatgaaatt tagagccaca caagcaaaca
    39961 tttccgattc acttggtaat attttgtttt atagcaatgg gggttggata gccgatgcaa
    40021 caggcgatac aatgattaat ggtagtggcc ttagtccaag tatatttagt actgtatata
    40081 gcgattatgg attccctcct cctagcatgc aaattgcatt gccttttccc gattcaaata
    40141 atgtgtatat attatttcat caaacaagtg agcctttgga atcaaactcg gttgaattat
    40201 attattccat tattgatatg aaacaaaata atgggaaggg tgtggtaacg caaaaaaact
    40261 tgattgcatt tcaagacact attttgtttg gaatacacgc atgtaagcat gccaatggtc
    40321 gtgactggtg gatagttaca cttcgtgata gtagcgatgt agctataaaa cttttattca
    40381 ctccaaatgg tattgcgcaa gtagatactc aacatttgat tgtgcctttg catcataacg
    40441 gttatacaca gcctatgtgc ttttcgcccg atggaaaaaa atttgcttat attgtgacta
    40501 cacaaacagg aaactcaaac tatccatctt tgcgcgattt acgaattttt gattttgata
    40561 gatgcacagg actctttaca aattcttttc attttgattt gaatgacaca tttccgggga
    40621 cttgtttgtc tttttcatca aattcaagat acctttatac agcaaaatat tttcgcattt
    40681 cccaattcga tttgctttcg gcatcaatac aagcgagtcg ccaatatgtt gcagattatg
    40741 atacgttttt tatgggaggc tatacagatt ttgcaaatat gtatcttggt gcaaacggta
    40801 aaatttatat tagttcaact agcaccacaa tttatttcaa tgttatagat tcacccgata
    40861 gttttggagt atcatgtaat gttttacagc actcattatt aagcccaaca tttctgtttt
    40921 ttaaccatgt aaaccaccca aattattatt tagggtgtga tacaagtttg ggttgtccgt
    40981 gcctctcctc aacaagccta acagaaaacg gccaacacga ttttcgtttt cgggtttatc
    41041 ctaatcctgt tgtaaataac tttgttaaca tagggtatat actacctcaa aataaaagcg
    41101 gcattttaaa actgtatgaa gtaaacggca aacaacttta cacgcaaggc ctgccacctt
    41161 ggagtaatga gcagagtata atattgccta aacttacaaa tggaatttat aatatgagaa
    41221 tagaaagtgg ggggtatttt aaatcaaaaa atgtggtggt gatgcatgag taataccccc
    41281 taccccctac aagggggcta aatgcaatac agttgtcggt taatagtatg ttttatttgg
    41341 agtaataata ttaataatgg aaaattaaaa gcaattttat accatgtata gtggagctaa
    41401 gccaattcat tttcgcatgg ctaaagaatt acgcaaaaac caaacacctg ccgaagaagc
    41461 actatggaaa gttttatcag gcaatacaat gagtggattt aaatttagga gacaacatcc
    41521 tatttcaaat tttattttgg atttttattg tcaccaatta aaacttggaa tagaaataga
    41581 tggggatata catagtgaaa caatgcaagc tgagtatgat attgaaagaa caaaaaaact
    41641 cgatgagctg aaaatagaag ttattagatt tacaaacgaa gaagttttga atgaaatttc
    41701 aaatgtgttg aatacaattg aatttgaaat taaaaaacgg aaacaaaaaa gtgacaatga
    41761 accaagtagc cacgtgcgtt gaaagccccc ttgtatgggg tttgggggta ttggcgtcaa
    41821 attataaatt aaaacaatgc acccaactaa gacaaacgta tttgtattaa gcccccttgt
    41881 agggggttgg aggtattagc gtcaaattat aaattaatgg aatgctacca actaagacaa
    41941 acgtatttgt attaagcccc cttgtagggg gttgggggta ttggcgtcaa aaattaacca
    42001 aacaaaaaaa atcaactaac taattctata tttaattctt atcccctcat tccaaagtta
    42061 taatttctaa acaccttttc ttactttcgt cattcaattt attttttgaa tgataaatcc
    42121 gcgattaagt ttttgggagt acgatcaata ttttaaaaat attgatgtgg caattattgg
    42181 tagtggaata gtagggctaa gcacagcatt acatataaaa acgcaatcac ctttattaaa
    42241 agttgtaata gttgaacgat cgtttttacc ttatggtgca agcacacgca atgccggctt
    42301 cgcgtgtttc ggaagtgttt cggagttgct tgacgatatg acaaaccgaa caagcgatga
    42361 ggtttttgca ctggtacaga agcgatacaa gggtttgaaa cttttgcgta aaactttagg
    42421 cgataaaaat attggctatc acgaattggg cggttacgaa ttattcactc acgatgataa
    42481 ttttttattt caacaatgtg aaagcgctat tgaaaacttc aacattgaat ttaaggatat
    42541 tacaaatgtg gaaagcacat acactttgac ggatgaaaag atttctaaat ttggtttcga
    42601 tggagtatca catttaattt ggaataaatg cgaaggccaa atagatacag gattaatgat
    42661 ggaatcactt ttagaaaaag tgcgaaatct cggagtaaca attttcaatg gaatagaaat
    42721 agaaaagtgg gaagaagcag aaaattctgt gatggtacat tgcaatgatg gattttattt
    42781 cgaggcaaaa aaaatgattt tttgcaataa tggctttgca aaaacaatat tgccacaact
    42841 cgatgtgcaa gccgcgcggg ctcaagtgct tattacaaat gaaattgcga atctgccttt
    42901 cgaaggatgt ttccattacg ataagggata ttattatttt cgaaacatag gcaagcgagt
    42961 gctatttggc ggaggtcgaa atttgaattt cgcagaagaa gccaccactg agtttggact
    43021 tacagagttg atccaaaacc ggcttgaaga aattttgaaa aataccattt tgccaaatca
    43081 tgaatatgaa attgaacaac gctggagtgg cattatggga cttggcaatt cgaaatcgag
    43141 catcgtaaag ccattaacca aacgaacata ctgcgcagta aggatgggcg gcatgggcat
    43201 tgccattggc agtttgattg gtgaagaagc tgccgaaatg ataattcagg aaatgtaaat
    43261 ttttagttta agcttgagct gttgggccac caaaattcat aggtaaatta aagccctcag
    43321 tttctttaat tacgccatgc atcttttcaa acttcgaaat gttatcggcc agtgcattca
    43381 aaagtcgctt agcatgttgt ggtgttaaaa gtatacgagc ttttactttt gccttaggca
    43441 cgcccggcat taccttcaca aagtcaacta caaactcact gttggaatgt gttattatgg
    43501 ctaagttaga atagatacct tcagctactt cttccgataa ttctatattc aattggttat
    43561 tgttgttatt ctcttgcatt attttttttt attaggtggc aaatgtaact gttatttttt
    43621 acttccatat atttttgttt gtttgctaaa tatgaaatca gaaacaaatt tatccccaca
    43681 gtttatcgag cagatgaaat tgcttctgaa agaaaatgcg caatcatttt tcgattcatt
    43741 acacacttca ccaccaacaa gtataagact caaccccgat aaagttgctt caactaaaat
    43801 tccatttgaa aaaattccct ggtgcgagca tggatattat ttgccaaaaa gattttcgtt
    43861 taccaccgac ccgttttttc actcaggtgc atattacgtg caagaagcat cgtccatgtt
    43921 tttgcaccat attttaaaat ttttgcaaat agaaaataag ccaaaacgca tacttgattt
    43981 gtgtgcttcg ccaggtggta aatcaacttt gattgcgtct gtaatgcatg atgatagttt
    44041 gcttgtaagc aacgaaacgg tttcatctcg cgttggtgcc ttaattcaga atatgatgaa
    44101 atggggtaat gccaatgttg ttgtaacaaa taatgatgct gcagcttttg gcaaaatgaa
    44161 aaattttttt gatgtcattg ttgttgatgc accttgttcg ggcgagggta tgtttcgtaa
    44221 agatgacttt gctgtgcagc aatggaatcc aaatttagta ttgcaatgcg aacaaaggca
    44281 aaaaaatatt ttgaacgatg tgtgggatgc tttgacgccc gggggttttt taatttacag
    44341 tacatgcacc tttaataaaa atgagaacga agatgtgctc aaacatttta ttgaaaataa
    44401 tgattgtaca tcatttgaga tttctgcgaa tcaattggaa aacatttcgt cttcgttcga
    44461 tgataaaata tttgccgatc gtttctttcc acacaaaacg aaaggagagg gtttttttat
    44521 ttccgtgttg caaaaaacaa gttccacaat tgttaccagc gaagaaaaaa aaataaaaaa
    44581 gcatgatttt aaaaagcatc tttttttgtt tctatcgccc aattttttag tcgctgtttt
    44641 gaatggtttt acaatcggag taaatcaatt gcatgcagag atgatagcaa acatggtaag
    44701 caaactaaac gtaaaaaaat ttggagtatg cattggaaat caggttggca gtgaaatgcg
    44761 ttatcatcac catttgagca tgtggccgca attaaataat agcgagttcg attttcttga
    44821 tttgaattat gaggatgcaa taaaattttt gcgtggacaa actgatatta attgtgactc
    44881 cggcaaaggg tggataattg caaggtataa aaatcaaaat ttaggttttg caaaaaacct
    44941 tggcaaccgg ataaataatt attacccaaa agaattaagg attatgaatc cttctttgca
    45001 agctaatgac caagaaatag tgagtacttt tttttaaaag tgatgtgatg cattaatatt
    45061 tcattccaag aaaaaacatc ataaatgcaa acacatcggt ttgctcatct atgagtttcg
    45121 atgttggttt tcctgcacca tgtcctgcat ttgtgtcaat acgaataaga gtaggcaaat
    45181 cgcctttttg tgcttcctgc aaagtggaaa taaatttaaa agaatgtgca ggtaccacac
    45241 ggtcgtcatg gtcacccgtg gtaactaatg ttgcaggata aattttaccc ggtttaatat
    45301 tgtgcagtgg cgagtattta atcaatgcat cgaattcgtt ttggttttca cttgtgccgt
    45361 aatccgattt ccacgaccat ccgattgtaa atttgttata acgcaacatg tccataacac
    45421 ctactacagg aatggcaaca cgaaataaat cgggacgttg agtcatgcaa gcacctataa
    45481 gcaagccacc gttggagcgg ccgctaatgg ccaacttatc tttgttggtg tatttatttt
    45541 ttataagata ttcggccgcg gcaataaaat catcaaacac attttgtttt tttaaaatgg
    45601 tgccggcttt gtgccatgcc tcgccaaatt cgcccccacc gcgaatattg ggcattgcaa
    45661 atattcctcc ctgctccaga aaagtaatac gctcaggttt aaactccgga gttttgctta
    45721 tgttaaaacc accgtatcca aaaagtaaaa gaggattttt tccatcgagc accacatttt
    45781 ttttatgcac aataaacatt ggtattgatg tgccatcctt actgttataa aacacttgct
    45841 ttgttacaaa gtcatcgggg ttatacttta gctttggttg aaaatgtaac aatgtttttt
    45901 ttgtgtcgat aaggtattta taaattgcag gtggagatgt tagcgatact gccgaataaa
    45961 aaaacacact atcctcttta tttccttcca ttgcatctat ggttgtatac gtaccttgct
    46021 taatgtcatt ttcgaatttt ccgttcaagt cgtaaacctt taaaatattg ctggcattgt
    46081 gcatgtattt cactattaat tttccacctg caaattttgc gctttgcatt acatcatctt
    46141 gttgcggaac aatatcttgc cagtttttat cggccgggtt tgcagcgtta actactataa
    46201 tgcggccttt aggagcattg ttggttgtga gaaaataaag tttgtcgcct tcgttattta
    46261 tcaaattata attatcgctg aagccttcga acagtggggt aaaagaagtt tcatttttat
    46321 aaagattttt aatcatcatc gcattgcccg aagtgctttc cgaaatactg agcagcaaat
    46381 atttttcgtc ttcggttgtt gttgcagaat atgttcgtaa tgggtgttca tcatcgtgat
    46441 aaaccaaaat atctttgtct tgtgtggtac caagtgcatg atagtacact ttatgatttt
    46501 catttttgcc ggagtatttt cccgtggtta cggcatcata acgcgaatag taaaagccat
    46561 atccttgcca tgcaatgttc gaaaactttg cccagtcgat agagtcggca agtgttttgg
    46621 ctgtgcgcac ttcttttact cttatttgtt gccaatcgct gccggctttt gcaactttaa
    46681 atgccaggta ttcgccattt cgcgatggtg tataatccga aagaaatgta ttgccggttt
    46741 tgtcaacttt ggcggggtcg agaaataacg tagccacatg caacaacgaa cgctgatacc
    46801 acaacgaacc ttgattgtcg agcccgttat tctttatata aaaatatgtt tgcccacatt
    46861 taaatggagg tagatatttt gggaaattcc aaagttgcgt cattcgtgat ttcagcgagt
    46921 cgcgaaaagg aatggttgcc aaaaaatcgt tggttacttt gttttgttgc accacccagt
    46981 cggctgtttc ggcactatta tcatcttcga gccatcggta tgggtcgctt atttttgtgc
    47041 caaaatattc gtcaaatact tcggttttct ttgtggtagg atattctaat ttttgtgcat
    47101 caacgttaag attatttaag ttgaataaca ctaacgcaaa gagaattgat ttttttgaag
    47161 tcatttcgtt tttaataatt ggcaaaaata attttaatac ttatggttga actaaatttt
    47221 gagttaatta atttaaaatg taaggctggt ttttgaaagc aaataatttg ctattactgt
    47281 gcttttcaat caaagtgatt tattttctta aatatacgaa gtttaagtat aactcttttt
    47341 gaacgatgtt gttattattt tagatttgca acttcaggta ataagcattt tattttctac
    47401 atttcggatt ataacaaaat aagtaataca agattagtgt tttcaaaatt aaaaaataag
    47461 ctaaaaagtg ttttttcaaa ttgcattttt agtaaagaag aaagtgatgt tgattatgtt
    47521 ttttttgata ccgttgccaa agtgcccgta gatgattgga atcggatttt taaaaattcc
    47581 gaatcgtgtt tatgtcaaag tcttgcttat ctccaatgcc ttgagtcttc cattacacaa
    47641 cccaatttgc tacgttatct tatcatttat aaaagtcagc aacctattgc agcattctat
    47701 tttcaattgt atgatgttaa caatatgagt gtgaaaaagc tgctcaatga tcaatatttt
    47761 aaggctcttt tagataaatt aaaagggctt aacgatgaaa agctttttgg taccaatgat
    47821 aaaaagcgtt ttgttgtagt aaatggcaac cttttcgcca gtgaatcttc ggggtttgcc
    47881 tgtaatgaag accctgaatc gctcaatgca tggcagtggg aaaaagttct cgacttgctt
    47941 atgaatagtg tgaatgaaaa ttcatcgctg ttgtgttgtg tgtcgaaaga ttttcaaaca
    48001 acggatgttg tgcttgccgg aaaattgcaa agtgccggtt tcaaaccttt tcaggtagat
    48061 cccgaaatga atcttaatat tcaatggaaa aattttgagg aatatctggc ttcacttacc
    48121 acaaaatatc gtacacgaat aaaaagtgta ttcgataaat cgaacgaaat agaagtggaa
    48181 gaattaagtg cagtcggtat aaaaaaatat ttcgggcaaa taaatgcatt actaaagaat
    48241 gttttcgaaa atgcccctgt tcgccttgca agtattgaag cgaatttttt catatgtcag
    48301 aaagaaaaat ttgatgacaa ctacatattc aacattttga aattgaacaa cgaaatagta
    48361 ggtttttata cagcattacg aaacaataaa acattgacag cgcaattcgt tggcttcgac
    48421 tatgcactaa atagaagtca taatttgtat taccgtatgc ttattgaata tactaaattg
    48481 gccatcgata aaaagtgtat gctattgcaa tttgggcgcg atgctttgga aataaaaagc
    48541 acacttggtg caactccttc gccactgcaa ctttttcttt ttgtgccggg catgtttgcc
    48601 tcttttttag ttgacccatt tttaaagatt gctacctcac gcgaatggat acaacgccag
    48661 ccttttaaac aaatcaatca accatcaaca ctcgatttga agcacagtgc gtaaactatc
    48721 cgataatcaa aagcggttta taaagacttt aaaacccggg caggtggtaa acgccaaaat
    48781 tatgtcgttt tcgccacacg gtacttttgt aaaaattgga atagtaaatg gcttgttacg
    48841 taatagcgat atctcgcacg acccagttca cgatgcagag gaagtgcttg ctataaacca
    48901 acgtagagat gtagttattc taaaagtgga ttacgaaaat tatacggtac aagttggcat
    48961 aaaacaattg cttaccgatc catggacaaa atttttagca aaatataaag tgggaagtgg
    49021 ggtagtggct accgtgaaaa atattatgga gtatggtgtt ttttttcatt tgattccggg
    49081 catttccggt ttgcttcaca attctgaaat aaagaagcat aatcttttac catcatcatt
    49141 tgaagtagat gaagtgcatc agctattgat aaaaaatatt gatgaagaaa ccagaaagat
    49201 agagctggag attgaaacgt aacaccaacg ggttagtttt caactaaaat aagccctagc
    49261 aatgttgatt aattaccttt taataaaggt ttcaagaaaa aataaaatga tgtaaatatt
    49321 actttgcagg ttgatttgca gtagttgaag gttgtgcacc agccggtggc attgtctgtg
    49381 gcattgaact tacattatcc tttaatttgc tttctttaac agtatccttt gcaacagttt
    49441 tgttatgcgc aggtaaagca aacgctgcga aaatagctaa taccattaat cccgtagcaa
    49501 agccccaagt tattttttcg aggccttcag tagttttttg cacgcccata tattggttac
    49561 cacccgaaag attacctgca aggccgccac cttttgaact ttgaataagg ataatgcctc
    49621 cgagtaaaat ggcagtaata agaagtgcta tggctaaaaa agtgaacata ttaatctgtg
    49681 ttttttattg tctttattat ttcggcaaag aaatgttttt tttcgggaaa agccaaactt
    49741 aatttttcgt atgcttttat ggctttttcg tttaaacctt gctgccgaaa aatatccgca
    49801 agggtttcgc ttacaaggtt ttcgtcccac tcaacacttt tgcgtgccat gttcgaaggc
    49861 ttataaaact cggcctttag tggctctatt ctggggtcgg cctttataaa gtgctcaata
    49921 atttcatcct tatttacatt ggcattttgt gaactgaaac gggattttgt gccttgatgt
    49981 tcattttgta acggcaagtc gttaagtccg tggtcatcat cattttccga tatttcatta
    50041 gatgccacct cttggttcca attgtttttg gattcctttt tttgaaatac ggtaagccaa
    50101 tcaaagaaat catgctgctc agtaacatta ttatttaaat ttatctcttc ttgctctgcg
    50161 tgggtttcaa aggcttgttc ctcaattgtt gaagtttctg ttttatcggc ttgcgtggca
    50221 attttttcta tatcaaaata tgcgctctct aattcaatat tcaaaaggcc aatttcctct
    50281 tcaacaaaaa catggtcgtt atctgttggt tcagcaacta taatatcatc aataatttct
    50341 ggttgtgaat cactatcatt aatattttct cccggcagtc catcagccac ttcgggtaaa
    50401 gtgtcgtttg tattcaaaac caaaccatcg ctcattaatg cttctatttc gaatttttca
    50461 tcctcggtaa cagcatggaa atctgactca tattcatcaa aatcaacact ttctttattc
    50521 gcaatcgcaa ttaattcgaa aaaatcttca tccgataatt gaggctctgt ttcttgtgag
    50581 ataacgattt ccgctgtcac gttttcttca aattcttcta caacattcgg ttggtcaatt
    50641 gatgccactg caacttccaa cggaatattt tcggtagcag ttggttgcga aaaatcattt
    50701 tcatcaacga caatttcctc ttctttatgt tgtacaacat tgttttcact caccgccaat
    50761 tcgtcttccg aaaatagttc tttcaatcgt tgattgataa gttcctcatt agcatctcga
    50821 tccaatgctt cgggtaatgt agcatcaata tgagcaataa tttcatcaac tttttcagaa
    50881 cgggcaatcg cctcaggatc agataattca ttctcattat tttctacggt cgaaatccgg
    50941 tcgctaataa tggcttctgc tacttcaact tcactatcaa cttcatcagt ttcattttcg
    51001 ttcaatatat cttcaactat taatgcatct acatgttcga aaacgggatg gtgacccaca
    51061 tcttctgcat ggctttcttc atctatcaaa gctggttcaa taggctcttt ggcttcggct
    51121 tgtacaagtt ttgccagcag cgattcatcg ttatctgcta ttacaatatt tgtatcatcg
    51181 attatttcat cagtgtttat agcctcaata ataaaatcac ctttttcaat ttcaacattt
    51241 tctacatcac tcaattgctc ctcctctttt ataaggggaa tagctttcga ttccatttcc
    51301 catttttcga cattgctttt gttgaatgca tgcttgggtt cgttttttgt ttgaatgagg
    51361 tcgtgcagtt tgctgcggtc gggtgcataa attgccgcat tttttatcgc ctttgcaaag
    51421 tcgattttat tttcggtgtg taatattttc gatttcagca tgtgggccat ctgaaaataa
    51481 ggatattgtt ctataatatt attgcaggta tcaatggcta catcgttttg cttcgaagag
    51541 ttgtaaataa tgtcgatgat gataccgctt tccattttcg tttgtagttg ttgatgcgca
    51601 aatatagttt tttattttgt taaactaata gattggattt ggaataaata gaaacctaat
    51661 tcggtgcaca acaaagcgca gacgccatga tgccgaatta attgaatttt aatttttgaa
    51721 agaaaaagca ctttgagcct atacagaaaa cattcttgta ttatttgctt cctgttattt
    51781 taatcctaat atttacaaaa ccaaaaagta ctttacatgt acaaagcact cacatacaaa
    51841 aacatactaa taccggcaag cgcgaccatg ctggcttgtg gccagctttt gtaacagcct
    51901 gctttaggtt ggtgttttca ccaacatgca ctgttccttc tttttttaca ttttacaaat
    51961 tttaattctt tatcaattat gaatacacgt tctatttttt attataatat cctaatagac
    52021 atggccgata acaatcaaca aattgaagac atagattcta tggatgtaca aacattgctc
    52081 aacatagcca cgcacaacaa tgcggtaagc tatatggcac aaagtgcatt gatgttagcg
    52141 ggcaaagcac atttcgatga ttggctgtta cgccctgcct atgggcaaag taatagaaca
    52201 ttggccgaag ccggtgaaaa taaaaccgat ttatttgcat ttgatattta cccaaatccg
    52261 gctaacagtg aatttataat acaattgcca aacccggaat tggtaagtaa aatttatatc
    52321 attaataata taggtgtaac ggtaatggaa tacaatgaaa atagtttgca aaagtataat
    52381 acgattgagg ccaaccgatt gacaaatgga atatacctta taagggtaat taacaaagac
    52441 ggtttcagct gctcccgccc cgttgttatt acaaattaat tcaaatcaat tagcaatagt
    52501 gcctgcttgc aggtactgtt gtttttattt cccacactta tgaaacaaat ttttttttct
    52561 ttgactctga tatgttgttt actctgcaac ttcgccacag cgcaatatac ttttaataaa
    52621 cagtatgatt attatgtaga tgcttcctca aacgttattg agacaaatga tggttatata
    52681 agtgctatca gtgcatggta caattttaat accatttact tagttgtgaa taaagtagat
    52741 aagaaaggcg ataccgtttt tcagaaagtt tatggcgtcc tcgataatga taattatgaa
    52801 gtatatacca tacaacaagt agaagacagc aattacataa taggaacact atatagcgat
    52861 tcaacaggct atgcagacaa ttttttgata aaggtagata agcatggtga tagtatatgg
    52921 atgcggaggc atcatgcacc gcagggttat ttatactttc cgggtcatgt taatattgca
    52981 agcaatggag attaccttat agcaagcaat attatggata gcattggcga tgatgtgaat
    53041 ggcggaatag ccaagtatga tagtagtttc aatttgtcat ttatcaccta ttttggcggc
    53101 atttatgacg ataatctact tgacgccatc gaactacccg accacagcat aatggccgta
    53161 ggatggacac gcagctatgg atttggcaac cccagcaacc gcgatatata cttagtaaaa
    53221 accgacagtg ccggtaattt gatatggcaa aaaacttttg gtaacagcga ctttgaatcg
    53281 gctaacacaa tttgtcccac ccttgacggc aattatatag ttacaggagg tcaccggcca
    53341 ggaggccagc caaaccctta taaaacatac ttgataaaag ttgacactgc aggaaaaaaa
    53401 atatgggaaa agactttctt gttctccggg caaaacgatt gccaccgatt acgcgaattg
    53461 gcaaatggtg atttgatagg ttgtggtgtt ggtacttaca atggtcaaga aggcggcctc
    53521 atttttaatt tggacagtac cggcaaagag caatggcacc gtttatatac tgtaaatagt
    53581 agcaatgcca tctttcgcga catacgcgcc actaccgatg gcggctttat atgtgcaggc
    53641 tttgcatttg taggcaacag tggcaatcag gatgccttgc ttgtaaaggt ggacagcctc
    53701 ggctgcgata gtgccggctg ccccttatac acagcaatag aagaaaccac ctcggcaccg
    53761 tccataacaa atgaagtgcg gtgcaatccc aacccatgtg gcgattttgc acacatacac
    53821 acacgcatgc taagcattta tggcttggta ctgtttgata taaatgggcg cgaagttgct
    53881 atccgccacc gtacaacatc gcacaatagc atacagctcg atacaagtgt gttaacggta
    53941 agtatatacg tagccaaaat attaaccgat ggtggcgatt tgtatgtgcg tgtggtaaag
    54001 ggtaattgaa tttttaaaag tataatacaa tgaaaaaaat gcccaatatt tataattcaa
    54061 acagtcatga aattgatttt acaatttcaa ataaaaatga aattgacata accgaacaac
    54121 ccgctgggct gtattttgta aaaataagtg atgggaagaa ggtgtatgtg gcaaaggtga
    54181 tgaaggagta aatgaagccc caacacttgg gtcgggatgc cgatcaaagt gtcgacatgc
    54241 aatagtttaa tggttaaaat ttctaattct tgcagctttt ttttctcaac cgtatgagtt
    54301 tattaatctt tgcacaatta ataatttcat tcacataaag tgggtttatc tcaatgccat
    54361 ttaatttgaa acatcaagct atttaacgaa atagaaattt gagcaatagt cattctttta
    54421 aaaaatagtt accagttcga tgtgcattta ttaaaaatat catccaccaa ttggcccaca
    54481 atattatcga ctaatttttg ctcctgtgtg gcaaaattga gactgctgga gtaatcggca
    54541 aatcggctaa aactggtttc gaaatttgct ttttcgtcct ttgaatttat aaatttcacg
    54601 tttacagtaa tggttaacct gttttgggct gccgtttggt tactttgtat ggcaaccgga
    54661 ccgttggcat aattgcttat ataaccttcg aaggcataat cgccctcagc actttttaac
    54721 gacagactgg attcattgtt ccacttcagc ttcaattttt cggtaaatgt ttggctcaat
    54781 acgggattaa ctatggatgc cctgttaggg aaaaactgaa cggaataagt tttcacttct
    54841 tttggaattt gtcctgcctt aaatgaataa caaccattga taagcattac tatacttaat
    54901 ccaagtatta gtttgtacaa acctatatgc gattttttgg aaatgtaaaa tagtttttcc
    54961 attatttaat accgtactca tttattttac gatagagtgt acgctccgaa atacccaatt
    55021 ctttagcggc atttttcctg cgatttcgat gcttttgcag tgcctttgta atgagttctt
    55081 tctctttgtc ttgaatagaa agcgactcct ccacaacttc gtgcggcttc atatcgtacc
    55141 ccggctttgt ttgaacggta aagccatcag gagtggtaat gtatggatta atgttgcctt
    55201 tgtcggaagg catatttgaa tgtgaaacag ggaagttctc acctcgggat attggttcca
    55261 tcgaggcacc ttgttcgtag gccacccgtt taagttcatt tatctccttg cgcatgtcaa
    55321 ataaaatctt gtaaagcatt tcgcgttcgg caccaaaatc ggaaatgccg cctttttcat
    55381 cacgaaataa aacaggcaag ccggcttgat tatctcgagg caaatatttc aataatgttt
    55441 cggctgtgag gtttttatct ttttctaaaa tcgaaatctg ttcggtaata tttttcaact
    55501 gacggatgtt gcccggccat ggaaagtcga caatcatatg catagcatcg ggcgaaattt
    55561 gcaacattgg catacgatat ttttctgcaa aatctatcga aaacttcctg attaacaatg
    55621 gtatatcttc ctttcgttga cgcaacgatg gaacataaat aggaactgtg caaaggcggt
    55681 aatataagtc ctcgcgaaac ttgccacgac tcatagcgtc tttcatgttt acattggtgg
    55741 ctcccacaac ccgaacatcg gttttcataa ttttacttga acccactttt ataaattctc
    55801 cggcttcgag tacacgtaac aatctaacct gggtggcaag tggtaaatcg cccacctcat
    55861 ccaaaaaaat agtgcctttg ttggcttctt caaagtatcc ttttcgcgct tcgtgcgccc
    55921 ctgtaaatga gcctttttcg tggccaaata attctgaatc gattgtacct tcgggaatag
    55981 caccacaatt tactgcaata taaggcccat gtttgcgggg gctgtaatga tgcattattt
    56041 gtggaaacac ctcttttccc acaccacttt cgcccattat catcacactt atttcggtag
    56101 gggcaacttg gcgggcagta tctatggcat attccaaaag aggcgaattg cctattattc
    56161 caaatctatt ctttatgtct tgtacttgca ttatgtttta ttaagatcct ttttcaacta
    56221 tttcgcccaa caaggttgcc gaagtgcaac catgaacttt cacgtataca taatcgccct
    56281 ttattgtatt ttgttttgga aacacaatca ttttattttg gtcgttgcgc ccacacatat
    56341 catcagtact ttttttcgaa accgcctcta ccaataccct gaacgttttg ccctcatcgc
    56401 gcttgttact ttgatgtgag agttcgcgct gtaagactat tatttcgctt aacctgcgat
    56461 tttttatttc ttcgggtatg tcatctttat agtttcgggc agcaagcgtt ccgggccttt
    56521 cgctatattt aaatgtgtac gaatagtcgt attgcgaata ttgcataatg cttaacgtgt
    56581 ccttatgttc ctcttcggtt tcgctgcaga aacctgaaat aacatcgctc gatattccgc
    56641 aacccggcac tattttacga atagcattta ttctgtcgat ataccattcg cgggtatagg
    56701 ttctgttcat tagtttcaat acacgactat tgcccgattg aaccggcaaa tgaatgtagt
    56761 tgcaaatatt ttcatatttt actatagtgt gtaatacttc atcggtaata tcttttgggt
    56821 gcgatgtgct gaaacgtata cgtaacaagg ggtttactaa cgctaccttt tcgagtaagt
    56881 tggcaaaatt gcaaaactcg ccattcgatt cgttcgtgta tttgtagctg tcaacatttt
    56941 gacctaacaa tgtaacctcg cggtatccat cttcaaataa taagcgcgcc tcgtttacaa
    57001 tgctatcaaa atcgcgactg cgctcgcgcc ctcgtgtaaa aggtacaacg caaaacgagc
    57061 acatattatc acaaccgcgc ataatgctta tgaatgcgct aatgccattt gtactcaatc
    57121 ttactggcga aatatcggca taggtttcct cgcgcgacag caaaacattc actgcctttt
    57181 ggcctgagtc aaccttgttt atcaaatttg gcaaatcacg gtatgcatcg ggcccaacca
    57241 ccaaatcgac caattgttcc gattcgagca aacttgtttt taacctttcg gccatacatc
    57301 ccaatacacc aactattaaa tcgggattat ttttctttac cagttttaac tcacgcaacc
    57361 gcccccacac gcgctgttcc gcattgtcgc gaatggcaca ggtattcaaa aatactacat
    57421 cggcttcttc cacattgcgg gtagtggtaa aattattgcc actcataatt gaggccacta
    57481 tctcggtgtc gctaaagttc atggcgcaac cgtagctttc aatatacaac ttacgcttat
    57541 gttcatcgcc aaagtttgta ggcagttcca tcacttcgcc ttgccgggtt tcgtctatat
    57601 atttttcttg catccgcttt attttttcaa accgcaaata taccagaaat tgggaggttt
    57661 ttttgaaaaa atattggttg gtaaattcca tgaacaaaat ggcaattatt tttttgaaaa
    57721 aagcttaaga tgtttcgaca acctgattca ttaaattggt taattgattt gtattgaata
    57781 aatttttcaa gcaatttcat ctcaaaaaat aatctaagca tccttacttt tttgtagcca
    57841 agattttttt aatatgttta atttcatcat tttattcatt agataaaatt gaagtcctat
    57901 tcaacaaaaa attttaagca catttattgc agtaataaaa ataggagggc aaacaatttt
    57961 taaaaaaatt tgattttcga ttaatcgtct atatttgccc gcccgaaaaa aaagggaaac
    58021 tgcagtaata aaaagtataa taacaaataa tggcgtcaaa tttagtaatt gtagaatcac
    58081 cggctaaggc caaaacaatc gaaggatttc ttggtaagga ttatattgta aagtcttcgt
    58141 atggtcatgt gcgcgatttg gctaaaagcg ataaagctgt tgacattgca aacaattttc
    58201 aacccaatta cgaaatcagc cccgataaag tggaagtgat aaaagaactc accaagcttg
    58261 ctgccaaggc tacttcggta tggttagcaa cagatgagga ccgcgaaggg gaagccatat
    58321 cgtggcattt gtttgaagca ttaaatttac atggcaaaga tactaagcgc atcgtttttc
    58381 atgagattac aaaaaaggct ataaccgatg ctataaacaa cccacgtcaa atagataaaa
    58441 atcttgttga tgcacagcaa gcccgcagaa ttctcgatag gttggtaggt ttcgaattgt
    58501 cgccagtgct atggaaaaaa gtaaaaccta gtttatctgc cggcagggtt caatctgtag
    58561 ctgtgcgtct aatagtagaa cgcgagcgag agattgaagc acataattct acatcatcat
    58621 ataaagtggt ggcacagttt aatgtgaagg ataataaagg aagtaacgta ctattgaaag
    58681 ccgaattgcc aaccaaatac aatactaggg aagaggctca acaatttttg gaacaatgta
    58741 agggtgctac atttacaatt ggtaacctcg aaacaaaacc gggaaaaaaa tcgccttcgg
    58801 caccgtttac aacttcgaca ttgcagcagg aagccagccg taagttgggt tttggcgtgg
    58861 cacaaacaat ggtagtggcg caaaaattat acgaggcagg gaagatatcg tatatgagaa
    58921 ccgattcggt aaacttatcc gattttgccc tcgatgcatc gatgcagcaa attgaaacca
    58981 gctatggaaa taaataccat cagcgcagag tatataaaac caagtcggct aatgcacaag
    59041 aggcacatga ggccatacgc ccaacatact ttgaaaattc gaccaccgat ggcgaatcga
    59101 acgagcgcag gttgtatgag ttgatatgga agcgcgccat agcttcgcaa atggccgatg
    59161 ccattttaga acgaaccatc gctacaatta atatcagcac cttggctaat aattttattg
    59221 ctactggcga ggtaattaaa tttgatggtt tcttgaaagt gtacatggaa agcaccgatg
    59281 aagatagtga agacgaagat acaaaaggac ttttaccacc attgtcaata ggccaggttc
    59341 tcgacctaaa taatattact gctgccgaaa aattcagcta tccggctgca cgatatactg
    59401 aagcaagctt agtaaaaaag ttagaggaac ttggtatagg tcgtccatca acatatgcac
    59461 caaccatatc cactgttcaa aaaagaggat atgttattaa agaagataag caggggagag
    59521 agcgaaaaat aaatatactc actttacaaa acgataagat tgaggcatcg gaaaaaacag
    59581 aaataactgg tgccgaaaaa tcgaaaatgt ttcctacgga tataggaacc gttgttaccg
    59641 atttcctggt aaaacatttt gatgatgtac tcgattacag ttttacggca agagtagaag
    59701 atgagtttga tgaaatatcg cgtggcgaaa taaaatggca agatatgctt cgtgaatttt
    59761 atgagccatt tcacaaagat gtattaataa ctgaaaaaga aggggaacgc gcttcgggtg
    59821 aaagaatttt aggtgtggat gccgcaacgg gcaaaaccgt tttagtaaga attggccgtt
    59881 ttggaccttt ggttcaattg tcgcctctta ctgaagagga aaaaccatta ttcgctaagc
    59941 tacgtccagg tcagcgcctc gaaacaatta ccatggaaga agccatggac ttattccgct
    60001 tacctagaac tattggcaag tttgaagatg aagatatggt agttgccatt ggtcgttttg
    60061 gtccttatgt tcgtcacaat ggggcatttt attctttagc aaaaactgac gacccttata
    60121 ccgttgatga agaacgcgct ttagagataa taaaaaataa aagacaggca gacatagata
    60181 aagtgataaa ggtttttgcc gaagatgaaa acaccagaat attaaaaggt cgttggggtc
    60241 cttttattgc gcatggcgac caaaacctta gaatacccaa aggaacagaa ccaagcgcta
    60301 taacttttgc cgaaatatta gaaattgcca aaactgcgcc tccttcaaag cctagatttg
    60361 gtgcccgttc aaaagctgct gccgatacaa aagcaaaacc atttaagaag actgctatta
    60421 agaaagcacc ggcaaaaaaa tctgccgcaa aaaagaaggc tttatagttt aactacttat
    60481 tactttttat ttgttagcat cttttcatgt ttgagaatca ttaagaataa acaaagttat
    60541 ttatttgggg aataaaatat tttgttcgtt tccactttat ttttcgattt caaatgaatg
    60601 acttctacaa aaatcattct gatgtaattt gaattaattt gtgaccgggc acatattttt
    60661 tatcaaactt caataaataa ttatgtcatt atctgaatta agaaattttg taaagccttc
    60721 tgttgtttat aaaaacaacg aatttgctaa aggaaccata ggtgataaag ttatacaatt
    60781 tgggacaggt gaatttgatt tcgataccac agaaccatgc attgcattgc ttggaattga
    60841 cgaaggccga ggatgcaaat ttcaatgcga ttatgcaatg ggttcggatg taattcgatt
    60901 tgaaattgca tcattaatgc aacattatgg taagcaccaa ataatcgatt taggtaatct
    60961 cattgtcggc aaaacagtta atgataccta tatggcagtt tcattattgt gtgccgaact
    61021 tatccgcaac aatatttttc cgattatttt tggtggtagt caagatttaa tatatggcca
    61081 atatggagct tacaaacaat tagcacaaat cattaatatt accaacattg atgctcgctt
    61141 cgatttaggt gaacccgaag aagggctgaa ctctcaatcc tatttgggtc atataataat
    61201 ggaacaaccc aattaccttt tcaactatag ccacataggt catcaaacat acttcacagg
    61261 tgaagatgct atcgaaaaaa tgactaaact atatttcgat tcttacagat tggggtatgt
    61321 tcgcaaagag atgcaagaca ttgaacctgt gtttcggaat acagatttgt tgagttttga
    61381 tatagcgtca attagaaact gtgatgctat ggcaacttac cttgcagccc caaacggatt
    61441 atatgctgaa gaagcatgtc aattgacttt ttatgccggt ttaagcgaaa aagtaacatc
    61501 gtttggcgtt cataattatg tgccgtcatt agatattaac caaaacacgg ctaaactagt
    61561 ggctcagatg gtgtggtatt atattgatgg tttcaacaaa cgcaaaaatg aatttccggc
    61621 attcaacaaa aatggatttc taaagtacat tgtttcactt tctgaaatag acaatgaact
    61681 catttttctc aaaagcctta ccagtgaccg atggtggatg gaaattccag tgcctgatgg
    61741 tcaaaataaa gttaaacgtc agcaccttgt tccatgttca tataaagatt atttatctgc
    61801 atgcaacaat gaaatgcctg aaagatggtg gcaaacattt aaaaaactca gctaataaac
    61861 tatttgtgaa aaaagtttca ctaatgtatt ttgtaattta taattaattt tatttcttta
    61921 ccgcgctcaa acaagcctag tacctaattt atcaaaaata tatgaaaaaa attacgtgtt
    61981 cattcctgtt aattttcggt tctatccttt acacttatgg tcagcaagac ccacagttta
    62041 gtcaaaatat gcataacaag ctttttgtga acccggcata tgcaggcagt tccggtgcat
    62101 tttgtggctc acttttatat cgctctcaat gggcagggta ttctggtgat ggtcaaccca
    62161 aaactggttt aataagtctt gatgcccctg tggatgtttt acatggcgga ttaggtttaa
    62221 ctttgtttca agaccaatta ggatacgaaa agactttcgg ggtaaagttg ggttatgcat
    62281 ttcgattcaa cgttggggct actggtaaat taggaattgg tatcgatgca ggatatatgc
    62341 aaaaatctat cgatggtaca aaattcaatc cgaatgatcc cggtgatcct aacatccctg
    62401 gtagtgttaa agcgaatgta gttcctgatt taggggcagg aatatattat aataatgaaa
    62461 aattatatat cggcttttca tcgtctcatt tagcagaagg aagctttgat ttaggggtaa
    62521 ccaaatacaa actatctcgt cactactatg gtactgctgg ttatagattt gatttatctc
    62581 cttcaattgg ccttacgcct tccgtttttg ttaaaaatga tggtgcaact acacaattag
    62641 atgctaatgt attggcacat tttaatgaga agttttgggt tggagcctca tatcgtattg
    62701 aagacgcatt tgtattaatg gcgggtttta atatttttaa tggactaaag attggttact
    62761 catatgactt tacatcttca gaattgaaaa attacagcga tggttcacat gaaatcatgc
    62821 taggatattg ctttaatcct aaaaagaagc caatattccg caatatcaat gtaagatatt
    62881 tgtagttctc atatattatt gttttatttg tttcctgttc aaatgttaaa ataatattat
    62941 tttacaaaat atttacattt atttgacgtt tctaaagata ttaattaaat ttacggcata
    63001 ataataccga agatgaagaa agtatttttt tttatttgca tcacatggtt gttttatggc
    63061 tgtggggggg ggaacaaggg tcagttgact ggagttctaa atagaccttc atggtatcaa
    63121 cctgatcctt atggtatggt ttacatccca tcaggctctt atgctatggg ccctagcgac
    63181 caagatgtgc cttatgctat tacatcgcaa acccgcaatg ttactgtaca tgcattttat
    63241 atggatgata cagaaataac taataatgaa taccgtcagt tcgttgattg ggtgcgtgat
    63301 tctattgcac accgattaat tggggaagag catattatta atgaaggtga gtataccgaa
    63361 atgctcaatt ggcgtaaaag aataaaatgg gatgatgagg ataatgttga aaagctcaac
    63421 gaaatgtttt tacccgaaag cgaaagattt tacaagcgta aagaaattga ttccaggaaa
    63481 ttgaactttg agtactattg gattgatttg aaaaccgctg cagctaaagt aaatagagaa
    63541 caaggtaaaa ccgatcgctc tgtatttata aagaaagaca taattaatgt ctaccctgat
    63601 actttaacct ggatacatga ttacacgtat tcattcaatg agccaatgac acaaatgtat
    63661 ttttggcatc cggcttacga tgactatccg gtagttggag ttacctggaa gcaagcacga
    63721 gcattttgta tctggcgtac tatgttgcaa aataattacc ttgatgccat cgaagaacca
    63781 tatcaccaag actatagatt gccaaccgaa gctgaatggg aatatgctgc tcgtggtggt
    63841 ttagaacaaa gtccttatcc ttggggcggc ccttatataa ggaatagcct gggttgcttt
    63901 ttaggaaatt ttaaaccaat gcgtggtaat tataccgatg atggtggttt ttattctgta
    63961 aaagtaacat cctattggcc aaatgatttt ggtctttatt gcatggctgg taatgtttcc
    64021 gaatggtgcc gtgatgcata tgaagcatct tcatataatt ttacacacga tttaaatacc
    64081 gattacgatt acgaatacaa agaagatgat ttgcccgcaa tgcgtagaaa agttattcgt
    64141 ggtggttcat ggaaagatat tgggtattat cttcaaacag gtacaagatc atgggaattt
    64201 ggcgacactt cgaaatgtta tgttggtttc cgttgtgttc aaagtttctt aggccgtgat
    64261 aaggcagatt tttaatctgt ttttttctca aattcaaaat tcatttatta aatttatcat
    64321 agtaattaat catttaacta tttaaaaaag tatggcatca ggaggattta ctcaatcaaa
    64381 aacatttaaa aacatcatgg taaagctcta tggatggggc gcagccgttg taatcgttgg
    64441 ggctttattt aaaattcaac attgggatgg ggcagatatt atgcttattc ttggtttggg
    64501 tactgaagct gtaatatttt ttgtgtcagc atttgaaccg ccacatcaag aagttgattg
    64561 gtcattagtg tatcctgaat tggctggaat gcatgaacct ggagcacaaa agaaaaagcc
    64621 tctggcaggt gctaaagatc ctgttgctca acaactagac aagatgctaa cagatgctaa
    64681 aataggtcct gatttaattc aaagtttagg aacaggttta aaatcattgg gcgataatgc
    64741 aggtaaaatg gctgatatat caaatgctac agttgctacc agtgagtatt ctgccaatat
    64801 taagaatgca tcgaagtctg taagcgaaat gaccacagct tatggaaaag ccacagaaac
    64861 aatgaatagt ttcaattctt catcttctga tttgaagact tataatgagc aaatgggcaa
    64921 agcatccaaa aacttgggag ctctaaatgc agtgtatgaa ttgcagttac aagattctaa
    64981 taagcatttg aaagaaactt ctaaattcta tgaaggaata aatactttga tgggcagcct
    65041 ttctgcatct ataaatgata ctcaaaaata taaagaagag gtaggtaaac ttgcaacaaa
    65101 tttaaccaaa ctgaatactg tttatggtaa tatgttatca gccatgagtg gaggtacaaa
    65161 ataatttttg ttacaatttt ataaatcgtt aaaaacagaa ccacatgtca ggaggaaata
    65221 tttcaccacg ccagaaaatg attaatatga tgtatttggt attaacagcc ttgttagccc
    65281 taaatatttc aaaagaaatc atcaatgcat ttgttacggt gaacagtagt ttggaaacta
    65341 caaataaaaa tacagatatt aagaacagta tgtcctttgc ttcgtttgaa aaatcaatgc
    65401 aaaatgaccc tgttaagact aagccacatt tcgacagagc gaagattgct caaaaattga
    65461 gtgatgatct ggttaaaagt atcgataaat taaaagatgc cttaatagcc aaaagtgaaa
    65521 aacttcaacc tggccaacca atcccaaagg ctgaaaatat aaagaataaa gaagactttg
    65581 atcaccctac tacaattatg tgcggaaaaa gtcatgatgg caaaggtgca gaggctacta
    65641 aattaaagga ccaattgttg aagtacaaaa aagatatcgt tgccacgttg gaggcaaaag
    65701 atcaagcaga aattagtaag cgtttggatg aaatatttaa tacaagtgat cctgatccaa
    65761 aatcagaaat tgtgactaaa gataacaagc gtacttggga aatggctaca ttctatcaca
    65821 accctgtggt ggctactgtt gcgatgttat caaaaataca atctgatatt aagaatgctg
    65881 aatctgaagt attgaataaa ttgttaacta atattaatgc tactgacctt aagttttcgg
    65941 atgttattgc taaagttgta gcaccaacaa gttatgtatt aaccggtcag caatataagg
    66001 cagatgtttt tcttgctgca tataacaaaa ctgctgatcc tgaaatttat ataggtggaa
    66061 gcaagctaaa tgtagaggaa ggaatgggaa aatatgttgt tacagcgcaa ggagaagggc
    66121 ttaaaaaatg gggcggggtt attaaagtaa agaagcctga taacacatat tctgagtatc
    66181 catttgatgc ggagtacatt actgccaaac ctgctgcagt tatttctcct actaaaatga
    66241 atgtaattta tataggcata gataatcctg tttctatttc tgtgcccggt gttcctaatg
    66301 acaaggtatc tgttagtact gaaggcggtg gtctatcttt gaagaaagta acaggtgagc
    66361 aatatattgc ccgtgctact actcaggctc aagatgccaa aataacttgt attggagatt
    66421 ttgatggaaa gaaaattaca atgggtgcac aaactttccg tgtaaaaaca gttccaaatc
    66481 ctattgctaa agttggaggc gtaactggag ggccaatgca gaaatctaca ttaatggcac
    66541 agcctggtat tactccggtg atggaaaatt tcgaatttga acttttcttt aaaattacga
    66601 aatttacttt tagcatgtac ggtaaaggta aagatcccat tgacttaagt tcatcttcaa
    66661 atcaaattac gcaacaaatg aaagatgcta tggcacgtgg aaaagcagga gataaagtat
    66721 actttgaaaa tatttatgct acaggtcctg acggttctac acgtaagtta gcaccagtga
    66781 attttgtaat tcaataatca atattaattt gtcaagtatg gtaaagcgat tgttttttgt
    66841 ttttgtgggc ggattgctct ctttaacggg gtattcgcaa aatgtactcg atggggcgta
    66901 tttgcgcgaa aactctcctg cactaaaagt tgtgccgttg gcatatctgc gtgaggctga
    66961 cgtaatgtgg tcgaaaaaaa tatggcgcta catcgattgc actgaaaaaa taaatttgcc
    67021 attacgttat cctcttgagg acatgacaaa tgacagaaaa agtttaatgc agttgctgtt
    67081 gacaaatgtt aatgaagggg cactcaccgc atatgatgtt ttggacgatg aatttaccaa
    67141 aagaatgact ttggatgaaa taaaagagag atgtggttca aaaaaggata ctcaacaaat
    67201 aacagacctt gatccccctt atttgactag agataccgtt gtagagaaaa aattttctac
    67261 tgacaaagta gtggggtatc gcttaaaaga ggaatggttt tttgataaac agcgtagtgt
    67321 attggatgtt cgtatcattg gattagctcc gatggttttt gcggaagacg aggccggaaa
    67381 taagattgaa ggtggcatta agaaagcttt attttggatt tactatcctg aagcacgtaa
    67441 cattcttgta aattatgaaa ccttcaatag aggtaacgaa gccgagcgaa gaacctttga
    67501 cgaaatatta atgaagcgga tgttcaatag tttaatttat aaagagtcta atgtttatga
    67561 tcgaaggatt ggtgactaca ctgttgcacc aattgatgcc attttggaat cggaaagaat
    67621 aaaaaacgag ttgatgatat tagaacatga tttgtgggaa tattagaaat aagttaaaat
    67681 acaagttagc accattttat ggtgcttttt ttttgtttag tatttcattt tgtagatgct
    67741 atttgaatat tttttttgct tcattccaat gaacatccat ctcggcaagt gtcatatttt
    67801 caagtttttg attaagcttt cttacttcat tttccatgta tttaaatcga ttgataaatt
    67861 tacgattagt taattcaagt gcctcttcag gatttatttc gtgaaagcga gcataattaa
    67921 ttagtgaaaa caataggtcg ccaaattctg cagccatgcg gtttttatta ccaagctcaa
    67981 cttcacattt aaactcattc atttcttcaa ttactttttc ccatacttgc gcggggttat
    68041 cccagtcgaa gccggcacca cgagcttttt cctgtatacg cattgctttg ataagagatg
    68101 gtaatgatga aggaacgcct tgcaaaactg atgtgtttcc ttctttcatt ttaagctgtt
    68161 cccaattctt ttttacagtt tcctcatcat tggcaatggt atctgaatat atatgtggat
    68221 gtcggcttat tagtttatcg caaatagcat tgatcacttc tgttatgtca aatgcattgg
    68281 tttcgctggc tatttttgca taaaatatta tgtgcaacag tatatcgcca agttcttttt
    68341 ttacttcatt taaatcttta tctaaaattg aatcggccaa ttcataaact tcttctattg
    68401 tcaagtgtct taacgattcc attgtttgct ttttgtccca tggacattgt gcacgtaact
    68461 catccattat ttgcaaaagc cttgcaaacg cttcctgtgc cggttggtaa ttcgtgtgtt
    68521 gcatcatttt acttttatcg aataagtaca tttgaaaact tcctgagccg gaagaataat
    68581 tataccttct ttttcaatca actgttgcga agaatcaagg ttatcagcta ttcccagcca
    68641 aggttcaagg caaacaaagt tggcattttt tgcactccaa attccaaaat atttaaagcc
    68701 atcaaaattc attgttacgg aatgattgtt ctttttattt ctgattgata tttctttcga
    68761 ttttagattt ttgaaaacta aggcatcttt atcgaatagt tcctttgaaa gctttattgt
    68821 tggaaggttt tcttccgtgt caatattttt tgaatccaac tgtaacgatt tggtttgcaa
    68881 tagtccgtcc tgcaatggga agcatcttag gttttccttc tcattaaatt gaataaaata
    68941 atcttcaaaa ttttcagatt cacttaacgg tacattaaag gccgggtgtc caccaataga
    69001 aaaataaatt gcttcactac ttctatttgt tatctcatag gtgatatgga gggtatcatc
    69061 ttcggccaat agatatttta caagcaaatg aaagtagaaa ggatatatag gaagtgttat
    69121 ttcatcatcg cttatttcaa agtgtatttc gtttggtgtt ataaaagcca aacgaaataa
    69181 tttttctcgg gcgaaaccat gccgtggtag tgtatatgtt tcttttttga aatgatatgt
    69241 gttgttttta agttgaccta caattgggaa aagtattggt gcatattttt tccatggctc
    69301 attagcttgc catatatatt cgatttcgtt tttcttatga aatattccgc ataattcagc
    69361 tcctataact ttaattttaa cgcgaatatt ttcattctca ataaattcta tcatataaat
    69421 tgttttgttt atacaagcac aaaacaacaa atttatttat aaagatgccc aagaaaaagc
    69481 gcaattataa gcctttgaat aaaatgtgtt ggtttacaag ccacagtact ttaatatgtg
    69541 atagaacagc attgcaataa tagctgtaac aggaatagtc agcacccatg cccaaattat
    69601 gttgaaagtg aggccccaac gcactgcaga cactcgcttc gtaattccaa ctccaataat
    69661 actgccggtt atagtatgcg tagtgcttac aggtatgcca agataacctg taccaaaaag
    69721 tgtaatggca ccggcagttt cggcactgaa cccttcgaaa ggcgtgagct ttgtaatttt
    69781 ttgacccatg gttttaacaa tgcgccagcc tccgaacata gtaccaatgg caatggccga
    69841 atagcatgca aaaggcaccc acaatggcaa gccgtgaatg tcggtgatag tgccatttgc
    69901 aatgtatgct gctgcaataa ttcccataac tttttgtgca tcgtttccac cgtgtcccaa
    69961 actatatgcc gctgccgata tcaattgtaa acgcctaaaa actttatcga cttttgatgg
    70021 agtgaatttt ttgcaaatat taatagtgat tactgaaatc aaattgccca taaacattcc
    70081 aattaaagga gcgagtacaa tgaagtaaat catcggcatc accttatcta attttacaat
    70141 gtcaaaacca gcgaaagcaa ttccggcacc tgcaaatcca ccaacaagag tgtgagatga
    70201 actactggga atgcctttcc accaggtgat gagattccaa aaaatggctg ccagcaaacc
    70261 cgcgccaata actgtaagcg acaggccttc gggttttaca actttagaaa cagtatcggc
    70321 aacatgcaat tcgaaaaccc agtatgcaat gaaattgaaa gatgcagccc aaatcactgc
    70381 cgaaagcgga gacaaaacct tggttgacac aactgtagca attgaatttg ctgcatcgtg
    70441 aaacccattt ataaaatcaa aaatcaaagc aaggccaata atccagtaaa gtaaattgcc
    70501 cataattaat tatgttttaa aattatggat tctaaaacat tagcagcgtc ttcgcattta
    70561 tctgtagccg tttcaagtgt tgacaatact tctttcattt taattatctt gattggatct
    70621 ttttcttctt caaataattg cgcaatagcc atttcgaaaa catcatcggc atgattttct
    70681 aatccggtta ttttcacaca cgattccatg accttattta agttgccttt tttttgcaat
    70741 tcgataatgg cgatatttag ttcctcggca gatttataaa ttatctcaga taatttttga
    70801 atgggcactg tagacttttc aattttataa agatccatcc gtttagccgc accatgaata
    70861 tggtcgcaaa tatcatcaat agcacttgcc aaagcatgaa tatcttccct gtcgaatggt
    70921 gttataaaat tacttcccaa ctctttaaaa atttcatggg ttatttgatc gcctttgtgc
    70981 tcaagcgttt ctatttgaaa taaaaattct tttacccgct cattattatt gaggcggctc
    71041 atttcataat ttaccaaagc tagctgtaca agattgcttg cggcttgttg aaagagtggg
    71101 aaaaattttc gatcgcgagg aataaaatac gaaagtagat ttgccatata ttaatttttt
    71161 aggcaatgtt aagaaattat tatggccaac aatcattcat caatctttga gaagattaat
    71221 ttttattaac aaaaatcgcg atcttgttta tagtttgaaa ttgatggcac cggtttaatc
    71281 atttattttt tacggtaatt tattctgata gaattgtgta gcgcttttat tttttgtttt
    71341 acgatgtttt tattttttat ctggaagtac ggttattatg tcacagattt tcgtagtatt
    71401 ttattatccg tctttctcca aaatactaaa acattatttt ctacattcgc ctccaatatt
    71461 ttaatcaatc tcaaaataaa atgagttatt ctaaaacaaa atttgcaatt ttttgcatgt
    71521 taataatttg caattggcaa ccatcatttt cacaaatacg tgatatgaat gcattgattc
    71581 ctatggataa aagtatacgc acaggagtat tgccaaatgg catgaagtat tacatacgca
    71641 aaaacaacaa acccgaaaat cgtgcagaaa tgcgacttgc tataaatgcc ggcagcacag
    71701 ccgaaaacga tgcccagcaa ggccttgccc atttatgtga acacatgtgc tttaatggaa
    71761 ccaagaactt taagaaatcg gaactggtag attaccttga atcgataggg acaaaatttg
    71821 gtgctcacct taatgcttat acaagttttg acgaaactgt ttacatgttg caattaccta
    71881 ccgataaaga agaaattgtt accaaaggct tgcaaatact tgaagactgg gcacacaatg
    71941 taacttttga tagcattgaa attgataagg aacgaggtgt agtaattgaa gaatggcgat
    72001 tggggcaagg agcacaagaa cgtgcacgca gacagtactg gcctgtatta tttaaagatt
    72061 cgcgatatgc ggtgcgttta ccaattggca aaaaagaaat tattgaaaat tttccacagc
    72121 aagtaataaa agatttttat aatgattggt accgccccga tttgatggct attgcggtag
    72181 tgggcgattt cgatttagat aaaatggtag cattggttaa agaaagattt tcgcaagtgc
    72241 ctgccgaaaa aaatcctttg cctttaaagg cctggaatgt gcccgataac gaaactatga
    72301 tggtagcagt agcaaaagat aaagaaatga cgaatactac catacaagtt atttataaac
    72361 acaatactca aacaccgggc acatatgaca attatcgtaa aggcatttta aataatttat
    72421 attcaacaat gcttagctcc cggtacagtg aactaagcaa acttgccgag ccaccatttt
    72481 tatacgctgc cgggggttat tcatcactgg tgagggccaa agatggtttt aatcttgtta
    72541 cagttgccaa gcccgatggc ggagcaaaag cattagaagc actgctcacc gaagcagaaa
    72601 gagttcagcg ttttggtttt accccttcgg aattggaccg tgcaaagggt gaaatgttaa
    72661 gcgatatgga gcgcgcttac aacgagcgcg acaaacagga atcgcgtgct ttggttgggg
    72721 agtgtgtgcg caattttctt gagggagaag cgatgccagg aattgaaagg gaatttgaac
    72781 tcaccaaatt gtttatgaat ggaataacgc tcgaagaaat gaatggccta tcgaagcaat
    72841 attttactga tggtaaaaac gaagttgtag ttattacagc gcccgacaaa gctgaaacca
    72901 aagtgcccac cgaagaggat gtaaaaagca ttatggctaa tgttaaaaaa atggatttga
    72961 cagcgtatgt agataaagtg tttgataaac cgatgctcga aaaaatccct gagcccatca
    73021 aaatattagc cgaaaccaag aatgagaagt ttaatattac ttattggaaa ttgccaaatg
    73081 gggcatccat ctcttttaaa aatacagatt ttaaaaacga tgaaatacgg tttcaatctt
    73141 ttcgatttgg tggccatagt accagtgaag atgcgaaaca acgttcggca atggttgcag
    73201 cggcacttat taactcgagc ggattggggg aacttgatgc caacgatatt gaaaaattta
    73261 tgagcggaaa aaatgccaat gccagccctt atgtgtcgga acttacagaa ggcataaatg
    73321 gcgattgtgc accaaaagat atggaactta tgtttcaaat gttgtatcag tattacatgg
    73381 cacctcgcaa ggatgaaggc atgttcaaat cgatgatggc aaaacaacaa gcgtctttac
    73441 aaaataaaag tgtttcgcct gagtctaatt ttcgcgatac tgtaagttat gtaatgagta
    73501 attatcaccc tcgcgccaaa ccaatgacag ccgaacgatt gaaagaaata aatttggacg
    73561 atgcatttaa tttttatcgc cagcgttttg atggtgtgaa tggtttgcac tttatgtttg
    73621 ttggaaatat tgatgaagct aaatttcgat attttgtaga aagatatatt gccacattac
    73681 ctgccggaaa ttcgaaacct gaatttgtga atttacaaat aaaagccccc gaagggaatg
    73741 tagagcggtt tatatacaaa ggcaccgaac ccaagagcag cgttagtttg cgcattacag
    73801 gtaaaatgga aaacaaccgc aaaaatagag taggtataaa tttgatgatg cgcttactga
    73861 gtataaagct acgcgagtcg atgcgcgaag ataaaagtgg tgtgtatgga gttggtgcaa
    73921 atgtttctac ctcaaaatat ccaaacgagg gttatgaaat caatataagt tttggatgct
    73981 cgcccgataa tgttgaaatg ctcattcgtg ctgccattgc cgaaattgaa ttgatgaaaa
    74041 aaaacggtgc ctccgaaatc gatatcacca aagtgcgcga aacagccttg cgcgaacgcg
    74101 agagtgattt aaaagaaaat aatttttggc tctctgcaat gtataaccaa tactattttg
    74161 gagacgatat caatgatttg gattcttaca ccgaattagt aaaagccatg gacagcaatc
    74221 gttataaaga attggccatt cagtatttga aaatggataa cttcgcacgg tttgtattgt
    74281 tgcccgaaaa aaaataaatt gaataacgtt tttcgcgttt tgggcaatgc aatatcaata
    74341 cataatattt cgttaattaa agtttgaaaa aataatgcgt aaaattttat tgtttctttt
    74401 tatactaact tctgtttcag ctattgctca aaagagtagt aataacaaac tggtgcaatt
    74461 taccggagtg gttgttacca acgatagttt gcaacccatt ccatttgcaa gtgtgatggt
    74521 aaggcgaacc aacacaggaa cttttaccga ctattatgga ttcttttctt ttgtagcgcg
    74581 caaaggcgat acggtagagt ttgcttgcct tggcttcaaa agtgccgcat tcactattcc
    74641 cgataccctg agcgaaaacc ggtattcaat cattcaggtt ttattgagcg atactatttc
    74701 tattcccacc attactgtaa tgccatggcc tacaaaagaa gattttaaac aggcatttgt
    74761 aaacctaaaa gtaaatgata atgatataac acgagccaac agaaacctcg ataccgaaac
    74821 acttgccgag ctttcggaat ttgtggcacc cgatggcacc attaattata agtgggcagt
    74881 gcaacaacaa caaactgcct tgtatcaatc ggggcaatat ccaagcaatg cattgttgaa
    74941 tcctattgca tgggccaagt ttattcaggc atggaaaaac ggtgctttta aacgcaaaaa
    75001 ataatacaac cactaccgta tacgaagttt aaacttatat gaaattttta tccttttctt
    75061 tttgttggtt ttttcttttt gtggctaaaa ctaatgcaca aacaatggca acggtacaag
    75121 ggcgtgttac cgataaaaat aataaaccaa tttattttgc aactgtagct gtgtcaggaa
    75181 cgcagtatgg caccacaact gattcgttag gaaattattc tttgacaatt cctgcaaacg
    75241 aagaattgat tgtagccttc caattaatca attatcagaa gcaagaacaa aaagtaaaac
    75301 ttacggtaaa cgaagtaata accatcaaca agcaacttat ttttaaaccc ggtgaaatat
    75361 taaccataac agtatacgca gataaaagaa aaaatccttt tgaaacaaaa attaatccgc
    75421 gcctgcccga agttattgcc ggtcctccga gcatcgaggc tgggctcaag tttagcggtt
    75481 tggtttttat tccaaatgaa ttgagcacac agtacaatgt gcgtggtggc agcttcgatg
    75541 agaatttagt ttatgttaat gatgtggaag tgtatcgtcc ttttcttgtt cgctcggggc
    75601 agcaagaagg tctctccttt atcaatcccg atttggccga tgatgtaagt ttttcggccg
    75661 gtggttttaa tgcacgttat ggcgacaaac tttcttcggt gctcgatgtg aaatataaaa
    75721 aaccaaaaga ttttcgtggt tcgtttaatg gttcgttgct tggtggcagt tttcatatcg
    75781 aaggaatttc gaaagatact tcgctttcat atttgctggg cgtgcgtcaa aaatcgaatc
    75841 agtacttact caattcgctc gatacaaaag gagagtttcg accatcggcc accgatgtac
    75901 aaacatatat aatatatgag ttgaacaaga gaaacagcat ttcgtttctt ggcaactata
    75961 cacaaaataa atacaacgtt atacctaccg acaggcaaac agagtttgga acggtgaacg
    76021 aggcgttgcg tttatcaatt tattttgaag ggcaggaaac cgataagttt aatagcggta
    76081 cggccgcagt tacttttaaa catacagtaa acgataaact taattttcga ttaatcacat
    76141 ccgcgttttc tacggtagaa tcagaatcgt acgatatact tggccagtac tttttagacc
    76201 agttagaaaa tgattttggc tcaagcgact ttggcgattc tgcaatcaat cgtggcattg
    76261 gttcttttct tacacatgcc cgcaataaac tcaaagcaca agtttacaat gccgaactgc
    76321 gaacaaatta tgtgaccgat aaatttttat tttggggcgg aataaaagtg caagacgaaa
    76381 acataacaga taaattgagc gaatggtatt atctcgactc ggcaggatat tctattcccg
    76441 gcccattcga taatgtgata gaattaaata atgtagttaa aacaaaaaac aatttaaact
    76501 caaacaggtt tagtgctttt gttgaatgcc aacaatatct cgactccaat aaaagattta
    76561 caatcaacta tgggttacgt tccaattact ggactgtgaa taagcagaat gttatttctc
    76621 cgcgtatatc tgcatcatac aggcgcaata gatttttatt taaaatggca gctggatatt
    76681 attatcagcc tcctttttat agggagttgc gtgatgcacg gggagtgctc aacaaagatt
    76741 taaaagcaca gcgttccatt cattttgtaa taggtagcga atacactttt cttatgtggg
    76801 gtcgcgagtt taaatttgta agtgaggctt actataaaaa tttgtcgagc cttgtgccat
    76861 tcaaaaccga agatttacgt ttgcgatatt ttgcaaataa caattccaca ggatatgctt
    76921 atggtgccga tttccgcctt aatggagaat ttgttcccgg tgccgagtca tggttttcgg
    76981 taaattattt acgcactttc gaaaacttga aagacgataa gtactatgat tattataatg
    77041 ctgcaggcga acaaatttta ggtggatata cgttcgatca ggttgctacc gatagtattg
    77101 taaaataccc gggtaacata ccacgaccta ccgaccaacg tgttacgttt tctattttgt
    77161 ttcaggatta tttgccaaag tttcctaagt ataaagtgta tttgaatttg cttttcggtt
    77221 ccggtttgcc ttttggtcct ccgggagatt ttcgttacaa agatattttt cggtacccgc
    77281 cataccgcag agtggatata ggtttttcgc gaacacttat tgacgaagaa gaaaacaaaa
    77341 agtataaaat gaaattgttg aatcgcctgc gttcactaag cataagcctc gaagttttta
    77401 atttgttgca ggtaaacaat accatttcat atttatggat taccgatatt actaaccggc
    77461 aatatgcagt accaaactac ctcacctcgc gacagttgaa tttgagagtg attgggaggt
    77521 tttagtggag gagtattggg tagtgagtat tgggtagtga gtattgggta ttgagatttt
    77581 ttagtattga gatattagta ttgagactgt tggtattgag atattagtat tgagatatta
    77641 gtattgagat attagtattg tgactattag tattgagact tttggtattg agttttttgg
    77701 tgttggtact tttttgggga tggggatttt caatattgag gcttttttta atttagattt
    77761 ttaaactttt aatcttaggc aattggttta atagattatt ttttcaaatc aaaagactta
    77821 ttaaaatgct ttaatacttt ttaaagtaat aagatttttc tcggtaccag cttgtttggt
    77881 ttccatcagg atagctatga acataacgaa tgataagaca atcatttatt attgttgctg
    77941 tccattcacc attatataaa caacctcttg ctttcactaa gggtataatg tttaaaggaa
    78001 atttttcact gtcgatttcg aaaattgcaa aaatttcatc gcccttgatt cttatttcgt
    78061 ccttggcaat agcgtaacta tttattgttt taccttgatg tgggtgtaat tctgtccagt
    78121 caaagccatg ctgaaattgt aacgtccaat ctgattttaa gtcgaaagta attttggtgt
    78181 tatcgggttt tgttttatta tcaagcattg cggcaatttc catttcaatc acctttaccg
    78241 aatctacatc aacaactatt gtcattctat ttctaaaaac agattcatcg ggttgactag
    78301 ttttttgacc ttgaactaaa ttggtgttta aaacagtgca caatagaata cagattatta
    78361 ttttcattgg attttgcttt tatgtttttt acaaagaaac ttttttgcca tgtaaaaatt
    78421 gtgaatttaa tttatggtgg aaattatagt tacgtaatga gagtgtcatg attacattga
    78481 ttcgctcgca atggtggaag gaaatgcatt attaataaat aacaaagttc aaagcaaacg
    78541 gggctaaaaa caatcctcac aacaaacgaa acaaagtact aagcagagat tgcttcgtac
    78601 cttacaatga cgtaacaaag aacaaggatt acagtaagca taacgaagaa tgcttgcatc
    78661 ccgaccggag tgtcggggcg aagaacaagg ctcaccgcaa acgtaacgaa gaacttggcg
    78721 catcgcaaac ggaacgaaga acatggcgaa tagaaaacgt aactaacaac taaattttct
    78781 tcctacacac cattttcaaa aaccagccgt ctttattttt attgtaaatc aatggaatca
    78841 ttttgtcagc aatgctcatt gcgggtgaat gtcgtgccat agtatcataa tatgtaaagg
    78901 gagattcaac cgaaatcaca tcggcattat agggtgccat catacttcgt atactgttat
    78961 ggttgtacgc ttttgtaccc atgctttcca tatgattcca tagcacccat gctttcgact
    79021 tccatggttt taattttagc aaagcatgtt ttacccaaaa aaaacgcgac aataccgagt
    79081 cgatattgta aaccataaac tttatttcgc caccgggttt acaaacgcgc acaagttcac
    79141 tataagcttt ttcggtattg ggcgagtgat gaattacccc ccagctataa accaaatcga
    79201 acgtgttatc cgggtactga atattttcgc agtcgcacac tttaaattcc tgagcgctca
    79261 aattgtacaa ttgcaaacga tgctgcacat gatttattcc ttcttcggta aggtcaatgc
    79321 cataagccaa tgcaccattt ttacaccatt gcaaaaagtc ggtacctgca ccaactccta
    79381 cttcgagtac ttttttgcct ttatactttt cgaattgagc aaacggtttt atttccggtt
    79441 cttttgtgta acgatattgc tctatttcat catagtattc cttggtatat ttttcgctca
    79501 cagctgcaaa agtgccgcac gatgctgcat tccaataatc gtgaacttcc tgttttaatt
    79561 cttcgctttg cgacattagt tttattttct tgtagtggcg aaaatactat ttttggtttt
    79621 aagtgttgaa tgcactccgt aaattaattt ttgccacaaa agcactaagg ctcaaaattt
    79681 ctcaaagtct tgaaggaagc attttaaata ttttgtggga tttggtgttt tggtgattta
    79741 gtggcccttt attattttag acatttcgga gtggactcaa tgttataaaa ttatgcattt
    79801 ggttttgact tctgccaata gcgaatatgt gcgaatattg tatgttcatt ttaaacacta
    79861 tactgaggag taatgtcgac caaagtgcaa gtatgtattt gattctccga gtaagaattt
    79921 aaagtattta aatttttgat ggttgaaata ttaaaaataa ataaattgat tttggtgaaa
    79981 ataaatgcca aaaataatga gaataaatct tagactcagc aaatagcaaa ttaagtggat
    80041 ggctgtttcc ctttctaatt ttagaatatt tttagcgata ttttgcgtcc aagtccatac
    80101 tctaaaagtt tatagagtgt agaaagttga atgtcgctat gtccattttc aattcttgaa
    80161 ataaaacttt tttttgtccc tgttttttcc gctaattgtt cttgtgtcat tgaggcaagc
    80221 cttctttctt ctctgattac ttcaccaatt gcaaatgcct ttgcttttat ttcgaaggca
    80281 gttcgttttg cagtcccaac ttttccgtaa cgcttatcga gatgttcgtc aaaagttgtg
    80341 atagttttac tttttcgatt ttttactttc attttgtttt agttttaatt tttcgtcaaa
    80401 atattctttc ataattttca cagccttttc aatttcttct gtaggtgttt tttgcgattt
    80461 tttttggaag ccgttgaaca agaccactac ctgtccctca tcaaaacagc aaaaaatacg
    80521 gtaaatgtta ctttgaaatt ctactctaat ttcataaagt ccattcgtcc cttctaaatg
    80581 ttgaaaaaat ttctgaggga ttctgtctgc aactgtcact acatacagaa cgtggtcaat
    80641 tttttctttc actttttcga tttgcttgtc aaagaagtca agaaaatagt ccttgaagta
    80701 aattatttat ctatgtcgtt ccattttttt gtctgcacaa agttaactaa taatggaaca
    80761 agcacaaaat aaaagttcaa atatttttgg cctttcggtt cgttctttaa tcgcatttaa
    80821 cccacgtata attaaaatta cttcatgcaa ctttgcgcta ctaattttat tattcttatt
    80881 ttgtggctca atttctcaac aataattttg gatttatggg ccatgttaat acgatagact
    80941 tttcaaaaac aaaaattctt ccttctatcg ttgaagatta ctttgctgat aagctacttc
    81001 ccaaatcact gtatcacgaa attccatctt tggatgccat cgaaaatgct attgcgctta
    81061 agcaattaca accagtaaac aggacagtat tagttgaaga attccacaag tcgtattcaa
    81121 gctatggatt caatagcaaa atatcaaatg atgaaagaac caacaaccca acaaccaata
    81181 acgaattaac aaataaccaa ataaccaatc accaattaac atcattacaa agcgaaaata
    81241 cttttactat caccacaggg catcagctaa atatttttac cgggccattg tacttcatct
    81301 ataaaatttg ctcgtgcatt gcattgtgca aagctgccaa agttcaattt ccgcaatata
    81361 attttgtgcc tgtatactgg atggcttctg aagaccacga catggccgaa atatcatcga
    81421 ccactgtgat gaacgaaaaa atagaatggg agcaaacctg gcaagggtgc agcggcaaag
    81481 cccccttgcc caattttgat tctttgattg aaagtataaa agtattgctc agtaacaaac
    81541 caaacataaa tgaactgata gctattttcg aaaaatcata tttgcaatca caaaatttag
    81601 cagaggcaac tttcaaattg gtgcatcagt tgtttcatgt ttatggtttg ataattattg
    81661 atgccgataa tgcaaacttg aaagcattgt gtaaagatat tttcaaaaag gaattgcgcg
    81721 aacaattttc attcaatgct ttatcagcta caaaccaaca attgcaaagc acttacgact
    81781 tgcaaataaa accacgcgaa ataaatttgt tttacaacgg accggaaagc cgcaaccgta
    81841 ttgtaaaaaa tggcgataag ttcgaagtgc ttaacactga tatttctttt accgatagtg
    81901 atgcatgggt ggatgcaaac tttgcaaaca ttagcccaaa tgtggtttta cggcctgtat
    81961 atcaggaatt tattttgcct aatcttgcat acattggcgg accaggcgaa gtgggttact
    82021 ggttgcaatt gaaatctacg ttcgatgcag ccaatgttac ttttccatta gttgtattgc
    82081 gcgattcatt tttattgctc gatgcaaaag cacaggaagc aatgacaaaa ttatcgctca
    82141 ccgatgaaga tttatttttg acatccgatg aattgagcag aaaatatatt gaaagtaaaa
    82201 acataaatgt catcgacttt gttgacttac aatcaaaggt tggtgaaatt ttcgaatcta
    82261 taagttcgaa gattggtgct gtggatccta ccctcgtgtt tgctgttggt ggcgaaaagc
    82321 aacgtacctc aaaagcaatt caggcgattg aagaaaagat tattaaa
//
LOCUS       DAORQA010000002        72238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_69_length_72238_cov_47.777011, whole genome shotgun sequence.
ACCESSION   DAORQA010000002 DAORQA010000000
VERSION     DAORQA010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 72238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 72238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72238
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69_length_72238_cov_47.777011"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..6568
                     /locus_tag="PK736_00365"
     CDS             26..6568
                     /locus_tag="PK736_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRC31875.1"
                     /translation="MLQPMASVTVCIYIPAGTYTVTVTDDNNCFSVISQLISQPDSIE
                     ITTTIVPTKCTDNNGSITASAIGGVGSLSFLWSTASTAQTISNLVSGTYTVTVTDQNA
                     CTVSKVVVVPFLSPPQLSIGSFTNVLCFGAANGVINVNIGNGNPPFTYNWSNGATTGS
                     TISNLAPGTYTLILTDSVGCIDSISQDITQPTAIQVTLAITNLSCGFQNGAIAASASG
                     GTGTLTYLWSNGATTSTINGLSAITYTVTVTDQNNCTKTASSTLIALPFPVANLITSG
                     NVSCFGGNNGFISIGISGGTPPYSILWSNGDTTINNINLTSGTYTVAVTDDDGCTATL
                     SHTITEPSLLEVSLTFTNAICGNNNGTIQSVTTGGVNPYSYLWSNSATTKNVNNLAPG
                     LYTITVTDSNSCTATASVNIVQVNVPVIQLDSVKNALCKNSLDGGIFISVAGAGPFVY
                     LWSNGFSTQDINGVGAGTYNVTVTDTNSCQSNFTQIITEPNAIVLSISKNDVTCNSNN
                     GNAVCNATGGTGTFTFVWSNSSTNDSIFNLAPGTYTVTVTDINNCTATQSVTINSTLL
                     PVITVSNIINVNCKGGDNGLITVTVAGGVPPYVYSWSNGNTTAEIDTLTAGLYILTVT
                     DFSGCSVIDSIVITEPDLLQLSLNSYPVSCLAIDGAIKCNVLGGTAPYTYLWSNSATT
                     DSIFGLNVGTYTVTVIDNKGCVATLSGQVVKAPGPQISLNNLVGIGCSGLPVGSIDIS
                     VSGGTIFTYLWSNGASTQDINTLLPNTYTVTVTNEFGCTSVDSFIVTGYFPIVLNTSI
                     TNETCGAGNGTATVTATGGNGVYTYLWSNGQTTSTATGLVANTYTVLVSDTGVCSNSK
                     SVTIINLANPVVTNDSIIKTLCSGSIDGAIYISVSGNGPFTYLWTGGSTLQDLINVAS
                     GSYTVTVTDTNSCVTTANFTIAPTLPLNLNMNAVSASCGVANGYAFVQVSLGTAPYTY
                     LWTGGATNDTISNLVAATYSVTVTDKNGCSDTASVVVNASNQILYNVDSVINVNCNGD
                     STGAIYVNVVNASVFSYQWSNGATTSNLLNVPAGIYTLSITGPNGCFADTTLTISQPL
                     PIISNITSTPAGCTLAYGTATVNPTGGNGTYTYLWSSGSTLNTAVNLLSGYVSVAISD
                     VKGCTLKDSVYVGISSQITVTSDSLINPKCAGGNDGAIYISVNNGILPYTFIWSNGVT
                     SEDNLNIPGGAYTVIVTDSVGCADTVSYNLFEPNALSLGFSSTPPSCIANNGSITVSV
                     SGGTPLYTYLWSNGATDDSIFNLSPSWYTVTVTDANGCTIQDSLLLSIPGAPVLSIDS
                     VTNVICGGAGNGAIYITTTGGTSPFNYLWSNGATDEDLLSVAGGTYTVNVTDANGCSF
                     SLSATISENTVLQAQVFTQNAGCNSNNGFAYVVTSGGTPPYTINWLFNGSSQDTIASL
                     FPGSYDVVVTDSNGCTTSATGIVNNSNAASIIPDSIVGVSCFGLNDGAIYISTIGGVA
                     PFNYLWNNGNTNEDITGLAQGIYTITVTDILGCITIFSDTVTQPDSIQFVFTVDSASC
                     NIANGSASLVVSGGTSPYTYNWSNGSQTQSINGVLAGIYTVTVTDAHGCSLSSNVIVP
                     NYSSPYISSSSVTGLTCYGSGDGFIDITISGGSPPFSFLWNDGNTNEDRSTLSAGTYT
                     VTVTDIHNCTALQVFVVSEPSDIVFSIQVQDANCNTNDGSATVTGLFGGVGPYTFLWS
                     NSVTISTISNVFAGTYTITVTDAKNCTKTAIASIGNISGPVITVDSISDILCFGGSNG
                     YISVNVSGGNPGYTFLWSNGGTTNTISNLTAGTYNLAVTDIIGCKDFYSVNFLEPAEI
                     NLNPLLAINNPPLFNISCNGAGDGSIVLNVTGGTFPYIYSWTNGASTSAIYNLSATLY
                     QVQVTDANGCLKKDTFLLTEPPPLMSVGGSDIEICGEDSVHLNATAPTYGYGFWSVLN
                     GTGVFDDDKSPQAFVSGLSFGVNTFMWIVADSSSCKDTSLVIVNVLEQIDAIAGSDRE
                     ICDDQVVLSATAPQFGFGLWSTFNSSGIIQDTTQALTNVFVLNVGSNVFQWTVYNGGC
                     KDSDLVNIIRLDSLDCLTPIDLPTGFSPNGDGTNDNYVVKGILDYPDNILIVYNRWGN
                     IVYQKTSYANEWKGTNNNGENLPEGTYYVTIKIKGFTKYVNSFIDLRR"
     gene            6579..7499
                     /locus_tag="PK736_00370"
     CDS             6579..7499
                     /locus_tag="PK736_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRC31876.1"
                     /translation="MKKIIFLFFYFCLQSGMYAQQDPLFSQYIFNGLVINPAYAGSHP
                     YMTLNVLARKQWFGMDKSPSTQAISWNGPLKSKKAGLGLILNNDRAGIIRQTDLFGSY
                     SYHLSLPKGKLNFGLSGGLSFYRSFLSDAVIWEAGDAVYAANSISNTYPNFGAGAYYH
                     NENYYFGISMPRVLSYYSQLKQGDDPTTVHRQKAHFYVTGGYMIETKSDIKIKPSALI
                     KYVKAAPIQFDINLNLLFNNIVWAGLSFRSGDAISLNLEYQLTRKLRVGYAYDYTISR
                     LRYSTSGSHEICIGYDFGYDIMKMKNPRYF"
     gene            7518..9425
                     /locus_tag="PK736_00375"
     CDS             7518..9425
                     /locus_tag="PK736_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRC31877.1"
                     /translation="MLSAHNYKFFILIFLIAILFSSCANIRYRTGNAYYDELLYADAA
                     KKYEMIGNKSKYEDVIFKLGDCYRLTGNNNKALSVYQRIVVQKNCKPIDRFYYAEALM
                     KAGRYKDAKNWFDSYLKNTNINDTRAQQLYKACDSLKVLYKDSALYVIQLMPFNANNV
                     SSYSPAFFKDGLVFVSDRSAPTSKNYRSGWTGQKYVDLFYTENLEGDNWSMPQPFADG
                     INSPYNEGPCIFNKDNNIIYFTRNSLDRGKPTVNAKLENNLKIYKGVLDNNTWKTIGE
                     LSFNNTNYSVFHPAIAQQGQSMYFASDMPWGYGGTDIYRVKFINGRWSKPENLGPEVN
                     TMGNEAFPFIVGDSILFFASDGKFGFGGYDIFEAEIENDKWVHAVNLGSPVNSSADDF
                     GLIIQKGGDKGYFASNRNSKTDRIYSFKKAPPQVSIIGTVTNFPENELIPNCKVKITS
                     TNNVDTVIDADSKGQFKFGLDLNQSYKFLVSDKAYYAAGFEVQTYGKKSSEIQEIPVA
                     LDHIILKKSKIYYKLKFETKTTVFKKTTQPALQELALVMKQNPQIKIEIVSHTDSRGD
                     DKPNMTLTLSRANSLKDALVLMGIDGNRIATKGMGESVILNHCKNGKVCIEEEHEDNN
                     RVEITVTSIDN"
     gene            complement(9715..9790)
                     /locus_tag="PK736_00380"
     tRNA            complement(9715..9790)
                     /locus_tag="PK736_00380"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9753..9755),aa:His,seq:gtg)
     gene            9939..11543
                     /locus_tag="PK736_00385"
     CDS             9939..11543
                     /locus_tag="PK736_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRC31878.1"
                     /translation="MKKKILFNVALLAIMATAQNANAQWSTAGNVLVGTEKLGSTNAQ
                     PVNIFTGNLNRMTIAANGKVGIGTTTPVNNLDIFSTGAVTFGIRSSTGGATMSMDKST
                     IASNAAFAYKYLGVPIWNSGCLGNNDFSIRNVVTNRFSMIIRANDNVGIGQNNPVARL
                     DVRSGSGVGDTIPAINATIGYTGLYDLPAVQATSEPSAGYGIGVEATGGWIGARGFGG
                     TTGVVGVSGLNGVFGIAIDQTGVENITGVYGAADGGDVSVGVYGNSQNATNNYGVYGE
                     QTDTAAADYAVVCLGDLFYWRAFAPSDARLKNNIQPYAGALSKIGMLNTSTYTFDHAK
                     YPGMALPGGKQIGFMADQLEKVFPEMVKNSDLPAGATRDSRGKLTYKTIKDVKTVNYI
                     SLIPVLVSAVNEQQAIIENKNAEMAQLKNEMAELRQQMNEIKQMVQTADRKSNSSTLG
                     LNNATPNPASGLVAFSANVPADMNNAEIQLVNSNGQIVRTVVLSGKNGLLSSTLNTAD
                     LANGTYTYTLYVAGKQVESKQLLISK"
     gene            11665..14775
                     /locus_tag="PK736_00390"
     CDS             11665..14775
                     /locus_tag="PK736_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRC31879.1"
                     /translation="MKQFFSTVLIVLCCICSNLAQQKENTLEIETKFIESLQWRCIGP
                     YRGGRSCAVAGDEKNKQTFYFGSTGGGVWKTTDAGNNWKNISDKYFGGSIGAIAVSKS
                     DGDIIFVGQGEQTVRGNVSEGNGIWKTTDGGRTWQFLGLKDTRHIVRIRIHPKNPDIV
                     YVAALGHLFGSNDERGIYKTLDGGKTWKKILFIDNQTGAADLVTDPLNFETIYATMWN
                     IKRTPYSLESGGPNSSIWKSVDGGNTWRNITNNDGLPSETLGLICVTVSPVNPDRVWA
                     MIEAKNGGLFRSDDAGETWTTVNQDRKLRQRAWYFSRIYAEPKDANKIIALNVQMFRS
                     TDGGKSFNNQINTPHGDHHDLWIDPEDAKRMIVGDDGGAQISNDGGITWSTYQNQPTA
                     QFYRVTTDNHFPYRIYGAQQDNSSVRILHRSSKGSIGRDDWQPTAGSESGHIVADPLN
                     DDIVYGGSYGGYLTRYNHKTGENRNITIWPDNPMGWGADSLKYRFQWNFPIFFSPNNP
                     KKLYAAANVLFSTIDEGHSWQVISPDLTRNDKSKQKSSGGIITQDNTSVEYYSTIFAA
                     CESPYEKDLLWCGTDDGLIQISRNGGGDWKNVTPKDLPEWSMVNKIEPDPFHKGGCYI
                     AATKYKSDDFQPLIFKTEDYGVTWKKITSGIAPDHFTRAVCCDTKIQGLLYAGTENGM
                     YISTNDGKLWKPFNQNLPQVPITDLTIKDDDLIVATQGRSFWILDDLTRIHYFLQNKE
                     QTNKEFFLIKPRDTWRLNSYVITSSTEGENPLPGVVVNFYIDKKNKKKQAELSFYDSE
                     MKLIRSFKSDTKSENEKLTIKNGMNHFSWNMQYPDAEKFDGMILWSGGTNGPYAPPGN
                     YTVVLKFGNDSAAQNFRIKHLPNLESTESDIKQQFDFLIKCRNKLSEVHKAISDIRGV
                     KKILNNLTEQNDSLLSEECKDSIQIALKELISIEEELYQTKSSSSQDPINFPIKLNDK
                     LAGLMSVAEESESKPSVQSLELFNVLDKSIQIQLTRLSKVFEQTIPEINKMVRNLNIP
                     YIQTKW"
     gene            14849..15637
                     /locus_tag="PK736_00395"
     CDS             14849..15637
                     /locus_tag="PK736_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRC31880.1"
                     /translation="MLRYLALFISGVVLFSGCSKTINPSIMFITPDDFPYTVDQTIGN
                     IEYRISPYDIIEFSIYSNDGEKLLERSVAANSNNAAAGLGNNPITEVGIKYAVDMEGF
                     VKLPQLGKTKLMDMTIRDAEKFLEEKYSQFIKKPFAILRVTNRRVIVFTGSGGLGSVV
                     NLTNENTTLIEALALAGGITTTGKARKIKLIRGDTRNPQVQLIDLSTVEGMKQSNLLL
                     QANDIIYVEPVPRISQGVLAEILPIVSIITSGLLIYNFVQQISK"
     gene            15637..18087
                     /locus_tag="PK736_00400"
     CDS             15637..18087
                     /locus_tag="PK736_00400"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRC31881.1"
                     /translation="MAKKKITAFNEDFDFKLFSTIARKNFIWVVVFIGTSLILGLLYL
                     RYTQPIYESASVIKLSSENKANTVLDISKNYFNEQQRNQMASDITLIKSKIISERALS
                     KLPLDISYFIRGTVLDFEMYRSSPFQVQYEILDSAMVGTRFELEFVNALSFQLFCVLP
                     NGTKISKEFPTVKWIEVPGLRLKVLPLDFKRIETEQADVKTNKAYYFVINDLKNVAQS
                     FSSQLQVNVLNPEAKTVQIKIQDFSPNKAADVVNAIANEFISYDVERNAAVANKIIEF
                     INSTIFNVESLLSNSEDSLERFKSETKVFNPTSSAGILSGQIIALQEKKFQLDLEFTM
                     YENLKKDVVANKDPGNLILALAGANDEQGITSMLTNLMQLMNEREKILIQTTTENAQY
                     RIIESQINSTINMIIRSINNQQDALNTKRAASDRQLKELENKYSLIPGQEARYGRLER
                     LFNINEKFYSLLLEKKAEFSITRAGYVAENILLEQAIAPTTPISPKRPLILSSALMLG
                     LLSSFILIVSRYLMHNLISSLDEVGQYTDAALLGIIPKYKREIPISQLLVDKNPKSII
                     SEAFRSIRTNLQFINAETGSKVMAVTSTISGEGKTFNAINLAGVIAFSGKKVIILDLD
                     LRKPKIHLGFNAENTRGISTILIGKDKVSNCILSSSLENLDFITAGPIPPNPSELILS
                     KRMDEMVDYLKTIYDIIIIDTPPVGIVSDGVPIIQKADYPLYILRANYSRKMFIHNIN
                     KMMDESKIKNLSIILNGVEMSKLKYGYGYGYGYSYGYGYGYGYGYGYGYGYYEEDKID
                     QSLFARFKRALPFAKKEE"
     gene            18091..18633
                     /gene="rfbC"
                     /locus_tag="PK736_00405"
     CDS             18091..18633
                     /gene="rfbC"
                     /locus_tag="PK736_00405"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRC31882.1"
                     /translation="MKVTKFDIEGPLLLEPKVFYDDRGLFFESYNELELAKAGITDKF
                     VQDNESVSKKNVLRGMHFQAPPYAQGKLVHVVFGSVIDIVIDIREESPTFTKYIMLTL
                     SADKKQLFWIPPGFAHGFLALEDNTIFRYKCTAPYNNASERGIIYNDSDIGIPWPGDN
                     FIVSDKDLVLPALKLSEKIF"
     gene            18636..19802
                     /locus_tag="PK736_00410"
     CDS             18636..19802
                     /locus_tag="PK736_00410"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRC31883.1"
                     /translation="MLEINSTIIKHNSLLIYGSYFLFSLTFSFLLNKLFLKFSSTLGT
                     KNNGDGSVVRWSSTAKPVLGGLSFYILFLLSVSIYFAFFNIGSSVSYSNTFIGLLLSM
                     AVGFLVGLADDAYNTKPFLKFSAQFACAFILIATGVYIKVFDNFYLNAILTIFWVVGI
                     MNSVNMLDNMDGITSTVSSFIILSVIILILLNRDLNNLHLIISIGVLAALIGFLFFNW
                     NPSKMYMGDTGSQFLGVFLSAFGILYFWNDYYVDLSGNYSLLKKFITVGIAFLLPLVD
                     TTVVVINRLRQGRSPFVGGRDHTTHSLAFLGLTDRQVAIAFGLLSLNSMFLVVIIKYF
                     ISQWSGVYELIFASYFFVILGFFFFATNKKIAAEKKIEQYSSTIIEKNLVSSPH"
     gene            19859..20770
                     /locus_tag="PK736_00415"
     CDS             19859..20770
                     /locus_tag="PK736_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRC31884.1"
                     /translation="MTLVICCYNFSGENNPAMEFRKQNNTFALSLPNHLQFAGEDLDF
                     SDAEIAERFDREMHINVYWQSQTIITLKKCARWFPAIEPILKAQGVPDDFKYLAVAES
                     NFSNVVSPSGAAGFWQFMPDAARQYKLIVNEEIDERYHVEKATVAACNYLKESKLKFG
                     SWLLAAASYNMGVEGVQTALQTQQVKRYQDLLLNEETSRYVFRIAALKSVIVNSEAFG
                     FHLKKSDYYTPYEYDILTTDSNITNLAEFALSKKINYKTLKLLNPWLRKNTLTLIGNI
                     PVPLKILKKDYRSFKMYDDKILNKDSI"
     gene            complement(20809..21432)
                     /locus_tag="PK736_00420"
     CDS             complement(20809..21432)
                     /locus_tag="PK736_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRC31885.1"
                     /translation="MAEAIWQGIILGFGLAFLIGPVFFLILFTSINKGFKPAVSIATG
                     VMLSDAFCIFLAHISNGFISQYPIVQKGAAIFGGGLLLVWGLQSILKKAELKTQADLK
                     NVVPTRNILRNLFSGFITNTLNPFALIFWIGVTGAIASKNFDESQNIAFYVSALAMVL
                     TTDIAKSYLAASIQHILNVKVIRWMNVISGAALIIFGIKVLMSIFMK"
     gene            complement(21447..22976)
                     /locus_tag="PK736_00425"
     CDS             complement(21447..22976)
                     /locus_tag="PK736_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRC31886.1"
                     /translation="MAILGSIIKKGIELRGLLKQDELNTPIHYQRRVLKKLLRKSQYT
                     LFGQTYVFTNILRSRHFVNSFRELVPIHDYTSIFAKWWNKSLHEEDVCWPGKVRYFAL
                     SSGTSDSSSKHIPVTRDMVKSIQKQSIRQIFTLRNYNVSEDTFQKGILMLGGSTHLNR
                     NGSYFEGDLSGITASKLPFWFQHFYKPGRRISKETDWATKLEEITEMAPNWDIGIIVG
                     VPAWIQLLLEKIIARYKLKTIHDMWPNLSLYVHGGVSFEPYKKSFEHLLAHPIKYIET
                     YLASEGFIAMQEAPDAEMRLNLNNGLFFEFIPYNEKNFTSDGEIIGKPQTLLINEVKE
                     GEEYALLLSSNAGAWRYLIGDVIKFVNKKNCEIVIIGRTKHYLSLCGEHLSVDNMNKA
                     IEMVSDELNISILEYTVAGIPDGGMFAHHWWIGTHDKADAEVLKLRLDEKLKILNDDY
                     RVERTSALREIYVDVVPVEVFYSWMETKGKVGAQNKFPRVMKKKMLEDWINYNKQYVT
                     Q"
     gene            complement(23118..23735)
                     /locus_tag="PK736_00430"
     CDS             complement(23118..23735)
                     /locus_tag="PK736_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRC31887.1"
                     /translation="MHLEIFNGRQNEMLPFIQPYTKNYYMVGGTAIALHLGHRSSIDF
                     DLFTIHPINKTKIKKAVSQSPFKSYIIVEKVDQIHFIINDVKLTFFQFPFIINATIFH
                     EKYFRIPELLTLAAMKAFALGGRGKWKDYLDLYFIIKGFYSITEICNEAIRLFPGVFS
                     PSLFIKQLSYFEDISFEEQVEFMPGFEVEIEEVKSFLVEAALEGF"
     gene            complement(23725..24009)
                     /locus_tag="PK736_00435"
     CDS             complement(23725..24009)
                     /locus_tag="PK736_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31888.1"
                     /translation="MENSPALKALIRKNAHLFWYTKDSEKENLPLPVVLEFFINYADK
                     ESIKELFEIVGIKNAAKVFFEEINKSERAANNYEKISRNFFTLYFLKYAS"
     gene            complement(24518..25849)
                     /gene="icd"
                     /locus_tag="PK736_00440"
     CDS             complement(24518..25849)
                     /gene="icd"
                     /locus_tag="PK736_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRC31889.1"
                     /translation="MSGEKITIANGKLTVPNNPVIPFIEGDGTGRDIWRASVRVIDAA
                     VNKAYNGTRKIEWLEVLAGQKAFDQTNNWLPDTTLDTIREYLVAIKGPLTTPVGGGIT
                     SLNVALRQILDLYVCLRPVQYFTGVPSPVKHPEKVDMTIFRENTEDIYAGIEYAGGSE
                     NNTKFLTWFQEAYPKDFKKIRFGTQQSIDEWMAVSGDKSSSNKVTVGIGIKAVSQHGT
                     ARLVRAAIQFAIDEKRKSVTIVHKGNIMKYTEGGFLNWGYEVAEKEFGDKVYTWSQWE
                     RTKKASGEAAANSEQDAAKAAGKIIIKDSIADITLQQVLTRPEDFDVIATLNLNGDYL
                     SDALAAQVGGIGIAPGANINYVTGHAIFEATHGTAPKYADQDKVNPGSVILSAEMMLR
                     YMGWKEAADLIIKGLNNAIANKHVTYDFHRQMDGATLVKCSEFGDEIIKGM"
     gene            26687..27667
                     /gene="moaA"
                     /locus_tag="PK736_00445"
     CDS             26687..27667
                     /gene="moaA"
                     /locus_tag="PK736_00445"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRC31890.1"
                     /translation="MLQDSFGRKHDYLRISLTDVCNFRCVYCMPAEDMQFMPPARLMQ
                     ANEIVDIATEFVKLGVTKIRLTGGEPLVRKDAADIILQLSKLPVELTISTNAVRVDEF
                     IDVFKRANIKSINVSLDTLNKEKFHTIAQRNSFDKVWSNINLLVENNFHVKINAVIMR
                     GINETELNDFVALTQQLPVHVRFIEFMPFTGNKWNDTKVFSYQQMLDVIATKFTYIKL
                     KDGKHDTTKKYKVLNHAGTFAVISTMSAPFCEGCNRLRLTADGKMKNCLFSKGEADIL
                     SAYRKGEDFIAIIKQCVHQKEKELGGQFTSDLHKVNSENILNRSMISIGG"
     gene            27817..28647
                     /locus_tag="PK736_00450"
     CDS             27817..28647
                     /locus_tag="PK736_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC31891.1"
                     /translation="MLKKIFLFSILSIAVQFCNAQGLQTFHSFTGTTILGDTVSFSQW
                     AGKKLLIVNTASFCGYTPQFTDLQALDSIYQANNFAVIGFPCNDFGAQDPGEDSVINN
                     FCTGLYGVKFQMMSKVGIALPDTAPWYKWLQWQYLNGVADAQVTWNFNKFCIDENGHW
                     VAHYPSNTLPFDTAIVNWILSPPANTSITEQTNISQVHLFANAAANKFELIIRAEKNT
                     DLFIRLINAQGAIISNLHKNILSGENKISFDAELTSGIYFVQINGANYNQCLKAIKLN
                     "
     gene            29079..30287
                     /locus_tag="PK736_00455"
     CDS             29079..30287
                     /locus_tag="PK736_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRC31892.1"
                     /translation="MKKQLTILFLLLLQSCLAITIYAQGGTWTWISGSSNLNSLGVYG
                     TQGVPSVNNHPPGVYETCEWKDKQGNFWIYGGIDFYSDLWKFNPTTLEWTWIKGNGFK
                     NQVPIYGTKGVANFSNTPGLIGFAATTWVDTSGNFWLFGGGYSSNALWKYELSTNMWT
                     WVSGSTNTNAYGIYGIKGIPSIFNSPGIRQETCSGWTDSLDCLWLFGGKGNGASSYGS
                     LNDLWKFNITNGEWTWMAGDLIVNVLSIYGIKGVSNFNNNPGGRWSYTKWKNMNGDFW
                     IFGGGEFQDVKNDVWKFQPALNEWVWMNGSTSVNSTGNYNISCLLSAFDLPFSRIENR
                     SSITDNCGRFWTFAGFRNNNNSALNDLWIFDPQQLKWVLLSGYNLTNQSGSYGILGIP
                     SLNNQPASRG"
     gene            30354..31157
                     /locus_tag="PK736_00460"
     CDS             30354..31157
                     /locus_tag="PK736_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC31893.1"
                     /translation="MTYCDMWVFDPDTNCISLNCNTNISTIANFSSSDSLFCEKQCID
                     FTDISQNNPTSWQWTFNGASPSTSSDQNPTNICYNNYGSFDVTLVACNQFGCDTITKN
                     NFITSYITPLDSIWFTNDTLFALPAYSYQWYEVGTGLIANATQNYYVPLTVGNYYCIT
                     KDTDGCESTSNVILYTSFFENDNHEISNAVWPNPANEVLFVNPQLLNNKNIIFEIYDM
                     QGRIILQQLLSNNKSTINIAHFAASVYGYRIMTNEKIIAQGRIVKEIKN"
     gene            31120..31542
                     /locus_tag="PK736_00465"
     CDS             31120..31542
                     /locus_tag="PK736_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31894.1"
                     /translation="MLKEELLRKLRIKNYEFGLRARNGLCPWFVSPHATTTFRATAFV
                     GVFSNNLISHDALIILHTAIVFTGNQLDTTFARQPRFVSSQTFIAMPFFALLMAGVFT
                     DHKNDNVTKYHGLKSMFTICPVPTELLLCVPVCVSNCA"
     gene            complement(31927..33138)
                     /locus_tag="PK736_00470"
     CDS             complement(31927..33138)
                     /locus_tag="PK736_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC31895.1"
                     /translation="MKKVLITFMLLFMYLYSNAQNLNWTQYGQQAEGASTFAWNNAKT
                     KLYKGTYYLSVHMSLDSGLTWQPIYQGLNGGVYSIAIKNDTIYGDFNNCFVGGIRYTA
                     NDGITWTRIDNGANQMPVTYDIATTNNAVVIAAYDQIKSTVDDGQTWFTKSTPINTFT
                     VATEGNAIIAGCSGGAFTLYYSNDNGQTYTSHVAQNMAGGWDLEFKDGVIYAGTTNGL
                     YRSNDTGKTWIYKGMAGQNVMGLGFYGQFGFASSANNSGQSTVFFSSDSGNTWVEENF
                     GLPVDPEILDFTFVDNKVIAGGWGSGMWSANLPTLAHLQESKKEETFLIYPNPATNTI
                     SIQIEPNQVTTNSSIEIFTLCGASLLKKSLNSIEDYTNIDISSILPGIYLIKCSGKDY
                     SNNQKLIVSKN"
     gene            33736..36828
                     /gene="dnaE"
                     /locus_tag="PK736_00475"
     CDS             33736..36828
                     /gene="dnaE"
                     /locus_tag="PK736_00475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRC31896.1"
                     /translation="MFLNCHSYYSLRYGTLPVERLVELGKQHGAEALCLTDINNVTGV
                     FDFVKECRAQGIKPIAGVEVRNGDTHLYTLIAQNNHGFAEINEFMTGYNLRHADYPLR
                     PYGFNYVHVIYTLDTYESWLGTGHKGTYTSTVNNEPMRTPLPGTDGGMAATMRIVRAV
                     EAPAESTDRGPKLRDYEWIGVRISRINRLVRADSSVYNKCVVMQPITYTDEHEYDIHC
                     LLRAVEHNTLVTKLEAHHVAPRDEHFTNVDHIRSVYAQYPQLLHNTEKLMDTCSFDFD
                     YGTIKNRSTFTGNRYDDKLLLEKLLLDGLEYRYGKNNREARRRVYSELDVIDKLGFTS
                     YFLITWDTIRYSMSRGFYHVGRGSGANSIAAYCLRITDVDPIELDLYFERFINAARTS
                     PPDFDIDYSWRDRDAVIDYIFKRYGSKHTALMGTISTFKGRSILRELGKVFGMPKGEI
                     DVLVNKPLDAANDTMLCKRIINYGNSIADFPNQRSIHSGGVLISDLPIFHYTALDLPP
                     KDFAVTQFDMYVAESIGFEKLDILSQRGIGHIQDSVKIIEQNRKVRVDVHDVKTFKTD
                     EKVNAQLRKGDAIGCFYVESPAMRGLLKKLRCGDYITLVAASSIIRPGVAKSGMMRAY
                     IQRFHKPESVVYLHPVMEELLKETYGVMVYQEDVIKVAHHFAGLDLTEADVLRRAMSG
                     KFRSRKELDKIVEKFFANCRERGYNDELSKEVWRQIESFSGYSFSKAHSASYAVESYQ
                     SLFLKTYFPMEFMVAVINNFGGFYRTWVYVHEARMAGAQIECPCVNTSTYLTSIKGRI
                     IYLGFIHIENMEQQYAERIAVERNRGGAFTSLEDFVLRIHISLDQVMLLIRLGAFRFT
                     GIGKKELLWQAHLLLNKGKVTMETMPMFIPETKTYPLPTLEHHRLDDAYEETELLGFP
                     VTCTRFDLLKTNFRGDISAAAMPQHIGKKVRLVGDLVAIKHVTTVKREYMNFATMIDA
                     EGNFFDTVHFPDQLKNHPFNGYGIYMLLGKVVEEFGFCMIEVEKMAKMELQADPRYAG
                     "
     gene            37227..38297
                     /locus_tag="PK736_00480"
     CDS             37227..38297
                     /locus_tag="PK736_00480"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRC31897.1"
                     /translation="MKNFLKTFLISISFFGVDFAHAQSNTNKFTPPRFDFEGGIETYM
                     LQKFDDAQIMIANINKDAYSASKFTITSDGTIQFVADSVWGNNNDLNNKILQYLKEGE
                     QYFKMPMINGNKVALTTYVYFDINYDSLANKYLPSFKLDYDLISLSSAYMNEGTKMIS
                     EMKYDEAITLFYEVYYTNINDMTAYYNIGLCKLKLGDTLSACAYWKETRKYNSAIANP
                     EIYEYCSAPNSIIEERMAEIKKDENSRYPEQVFTIVEHMPDFIGGEKELKKFVQTRFH
                     YPNVEKEKNIGGTVYVTFIVMPDGSMNDLRIVRGIAGAPFLDQEVIRLVRQMPKWNPG
                     SQSGKIVPVQFNLPIKCSPITN"
     gene            38419..39462
                     /locus_tag="PK736_00485"
     CDS             38419..39462
                     /locus_tag="PK736_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRC31898.1"
                     /translation="MKLKLNLLFVLMLLFMQVDLNAQQADSIKYQPPVFDYEGGIKAY
                     INSKLEYSKELNKKYPEGMDWVMNFMITRNGMIEFDMRSMPDTNNMFEVEVYNILQQG
                     RSYWKAATLDNAVIVTDCDMNMNVHYNKKEKTVSADINLRFDKNKLAENYMDVALTKL
                     KASEYKNALPYIQAASDLSPGNMERKLQLGICKMNIGDTVEACEIWRKIKASNIKIAD
                     DQIAKYCGAIAKKNDDENKPNTEIKTVDEVMPEFPGGQAELVNFIIKTFHYPEKEREA
                     NIEGTVYVSFVVKKDGNITHIRIKRGIKDGLGLERESIRMVKCMPRWKPGTQNGKPVD
                     VQFTLPVKCKLRG"
     gene            complement(39578..40222)
                     /locus_tag="PK736_00490"
     CDS             complement(39578..40222)
                     /locus_tag="PK736_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31899.1"
                     /translation="MKIIAIREDTSALFKMLNPEVLSTSNIGISGGISSVGTNVGLEN
                     FKILWRLDSGKYSMVWEVILQSIKIGGTYGSPYDSTREIFTIPYMNAMKHLGPFFGDF
                     HPKYEIGNIDTLLGSFICFKPHVPAYEKPDSTSKIIAYLTYDLVELDRYKSYQASQDS
                     LAKFQCCFNFVTTLDKKTKGWIYEDYCCILKRANSIVIEKINHQWKISIFGLFD"
     gene            40665..41531
                     /locus_tag="PK736_00495"
     CDS             40665..41531
                     /locus_tag="PK736_00495"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRC31900.1"
                     /translation="MVFSVSDSGVLQFDKSYEQFDIKTFCNAVYTMLKQSTHLWKPAL
                     LGTNKINSIMGITFAVQHIDKEQNIIYNPEILDTDTTWLANVFYNEGTRLLNDKKHFE
                     AIPFLEKATEINSSDDYAIYNLGVCKLALGDTISACELWWQTISLKSKIANKIIFKYC
                     PNKFYFAKNEDGSIDSTIHFYTIIEQMPEFPGGANGLINYIQENFRYPTESRLNNVMG
                     TVYLTFIILADGTITRVKIKRGIPGGEDLEQEAIRLVKSMPNWIPGKQNGKFVNVQFQ
                     LPIKCQLTDYHK"
     gene            complement(41590..43086)
                     /locus_tag="PK736_00500"
     CDS             complement(41590..43086)
                     /locus_tag="PK736_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC31901.1"
                     /translation="MKKITSTLFLLIIIVKFSLAQSGTLDPTFGSGGIFLSNGAPYGQ
                     PLGMAIQPDNKIVVLGNYSNFLMLRLNTDGSYDNSFGTGGQVLSPVAGSGWAVAIQSD
                     SNIVVAGFNNSTQASVVARHLTNGNLDNTFGTSGKVVTKVGGDCSSKAVAIQSDGKIL
                     AAGMTSTGGTDMMVLRYNTNGTLDSTFDNDGIVTTALGFSYDYANSVSQQPDGKIVVA
                     GSAGNSTFDFAVVRYNSNGSLDSTFGVNGKVATDFNNSDDQGYGMKLQSDGKIVVAGT
                     SSANGNYAFAIARYNTDGTLDNNFGTNGKTITGAPGHNWYCNAVDIQQDGKIIAVGRS
                     PFASANEMFTTVRYDSTGILDNAFGTGGIVFTDLTSADDYANGVAVQNDGQILVVGQA
                     RYQTYWQYAVVRFNGDMAGINDYGLQSNSFLISPNPASDNFNITFNETLKSGIKNINI
                     FDLTGRNVFQQELTKNKSENFRCNFPPGIYLVQISDGEKVFNQKLIIK"
     gene            complement(43314..44519)
                     /locus_tag="PK736_00505"
     CDS             complement(43314..44519)
                     /locus_tag="PK736_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRC31902.1"
                     /translation="MIFVLGLTMLVSLHVFSQSENNNSRQKPRRPAMKLLEQKKNKLD
                     SLATFKRNERIRLENENAQLEKEIEQQKQLYTTELSDADNELQKQKENINRMSADQAR
                     MQLTLNVQKMLADSLAYASRIDSLLIKGQTAKITQQTIALENANTKEALQKSQRNLFF
                     VVATLGLLLAAAMYYRYASANKFNKQLLVEQEKSENLLLNILPKNVANELKQTGKAQA
                     QNIPQATVMFTDFVDFTKVAESLTPTQLINELDYCYRKFDAIMTKYTIEKIKTIGDAY
                     MAVAGLDNDNQSHIRIVNAAIEIIDVMIAHQRDCEKLGKPFFKIRIGLHTGPLVAGVV
                     GEKKFSYDVYGDTVNIAARMESSSEEGKINISTTTYNLVHNNFACTPRGKISAKNKGE
                     MEMYFVEKI"
     gene            complement(44667..45680)
                     /locus_tag="PK736_00510"
     CDS             complement(44667..45680)
                     /locus_tag="PK736_00510"
                     /EC_number="6.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase (ATP)"
                     /protein_id="HRC31903.1"
                     /translation="MRKLASIQKIKNLEPIEGADAIEKATVLGWHLVVKKNEFQIGDL
                     CVYCEIDSLMPNKPEFEFLKVRGMRIRTIRLRGQVSQGICFPLSILDASVKVEEDVDV
                     TEVLGITKYEPPIPACLAGIMKGKFPSFIPKTDETRVQILQNVLDRYKGTKCYITEKL
                     DGSSATYFVKDNIFGVCSRNMELEEDNANSFWKVARENDLENKMKLLNINIALQGELV
                     GEGIQNNKCKIRGHKVFFFTAFDIDNFRYYNFEELENILQKLELPMVPVINRDYILEN
                     DIEAIVKLATIKSTMDAQAWAEGIVIRPYDDKVDAELSNTFSNGRITFKAINPEFLLK
                     YGE"
     gene            45834..46082
                     /locus_tag="PK736_00515"
     CDS             45834..46082
                     /locus_tag="PK736_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRC31904.1"
                     /translation="MKLFIKNIESSINEAVLESLFALYGEIESTKIIYDRITWESKGY
                     GFIEFTKKADGQKAIEAMNGKEIRGKALSVVEADEKRK"
     gene            46424..46496
                     /locus_tag="PK736_00520"
     tRNA            46424..46496
                     /locus_tag="PK736_00520"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46457..46459,aa:Lys,seq:ttt)
     gene            complement(47051..48211)
                     /locus_tag="PK736_00525"
     CDS             complement(47051..48211)
                     /locus_tag="PK736_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31905.1"
                     /translation="MQIIEVTTKEQAQQFLQLPLQLYKNDKNWAQPLDKDIDEVFDKE
                     KNKFFRHGICARWLLADNGKTIGRVAAFVNHKLAKKNDQPTGGMGFFECINDQKAANI
                     LFDTCAEWLKEQGMEAMDGPINFGERESWWGLIIDGFNPPQYKMNYNLPYYQTLFETY
                     GFKDYFQQHCYAIEQLEDLPQKFYQRHAEISKIPEYSFRTLKMNEIEKYAEDFRSIYN
                     QAWVQHGSGRDLEQKQVQGFFKKLKPIIDPDIMAFAYYKNDPVAFAICLPDINQLFRK
                     LNGKFGLWQKIKFFVMLKMHVCTKMIGTVFGIIPAHQGKGLDAYIFVEMEKIVKRKKS
                     YDEFEMQWVGDFNPRMLNIAKEFCPVRSRVLVTYRFLFDRTKEFVRHPVIRG"
     gene            48266..49063
                     /locus_tag="PK736_00530"
     CDS             48266..49063
                     /locus_tag="PK736_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC31906.1"
                     /translation="MKNKVVVITGASSGIGEALAKKFDAMGSNIVLAARDEAKLMAVK
                     NSLQNKSIAVVTDVSNENDCKLLMEKAIAEFGSIDVLICNAGMSMRAMFNDVGLEVLH
                     KLMNVNFWGAVNCCKHALPFLLKSKGSIIGVSSIAGKVGLPARTGYSASKFALEGFLN
                     TLRIENLKNNLHVLVACPGYTASNIRNAALNEKGQQQMDTPLDENKLMTADEVATEIY
                     MATMERKRDLVLTTNGNFTVWLNKFFPAWMDKIIYRFVAKEKDSPFK"
     gene            complement(49225..51846)
                     /locus_tag="PK736_00535"
     CDS             complement(49225..51846)
                     /locus_tag="PK736_00535"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC31907.1"
                     /translation="MKKILVFIFVTLLANYSRSQCNDNVDSLIKTASRDNVITYRMKH
                     DFKLTTDNIIAPKIFTLAFNHAWAKESGCSIPVFILTPQNINFNNFGLNVLGVDSSIT
                     NNGNPVKWESYQIENPDTIYEAHANAGSCHSHSHANSCVAIGSAEFASGCSGVIQNYG
                     LAQAISDSTCRAEAYSFSVAGVTVVEDKDLLKGNIDWEAFFECGKKGKGHTIIQKSGS
                     NDPINFYIKDSITGKDTLGTLLSIHFSFYTGLDTLTQLNYWRNDTVHIESDSSEFKII
                     YPSNLTSLNGSLIINVNGGMVSSIVKTGNYSTFNQVLVGQFLPLHFYLNDSLHFHYNL
                     DSMQLLNSMGINNQDSLHVRLFMFGNCENNYAMDTTLINLAPVVVNATCANVANGVLS
                     VTATLGHAPYTYAWSNGATTATATGLAEGTYTVIVTDVIGCTASSVNIITAPNALTAT
                     CTSTNVTCNIANNGTASVSSSGGSGNSSYSWSNGSTGTFVSGLSQGTYTVTVTDANGC
                     TNTCTSVITSTGTPPAPPGLITGTTALCRNTIGNTFSILPVAGATSYTWTLGAGAIIT
                     QGQGGTVITFSLTGSALPGIVSVTASNACGTSTSSNLNYTVNSIPGAPSTISGQTSGV
                     CNKPNVPYSTATVPSATSYFWTVPTGASIMSGQGSNAVMVSFSNSFTGTGNITVKAVN
                     TCGMSAARTLTLNARPQSPIISGLNYACKYQPGLHYTVAPVAGATSYTWTVVPGSTIV
                     SGQGTPHIVVNWGNINGIITCKANNSCGSSAASHLAVAFTCKNENETIDPNSISFYPN
                     PGKGRITLQYESYTDGEMEMGVYELTGKIILNKKLVSAYGNNKHEIDFANLSSGIYLV
                     QLKVDGTNKWMKLIIND"
     gene            complement(52026..55007)
                     /locus_tag="PK736_00540"
     CDS             complement(52026..55007)
                     /locus_tag="PK736_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC31908.1"
                     /translation="MKKTTSKQVQLNVSLSCIRPIIICCCFIMAATFNAFSGNALKDN
                     AASPNCENYHSPNCDNGTVTTNLMAPNFFVAVNGTNVLCNGGATGTATAVTNGAAPPL
                     TYVWTNGASSASVGSLTAGVYTVIVTDFNGDTGSASLTITENTALSITETHLNPSSTI
                     ALNGSITLSVNGGTGGYTFSWNNGSTNQNLTGLGAGTYNVTVTDNVGCTSSLSIILAP
                     SSVMTVNFTTSSCGDFQCANVVGGTPPYTYLWCNGTTLPCINLSSIQPCTPMCLTVTD
                     AGGFSIFLNVIRPNVTFTAIPPSSCNSCDGSITVNTTCLCSPSYAWSVSPLQIGPTAT
                     GLCAGTYCVDITDCNGTVYQCCTTIVASTCVMNIGFTYGGCGNSILANVSGCSGLYTY
                     LWSTGATTNTITGTIPCSKYTVTVTDINGCTASREALVPSVVFKEVCGGLCIDTLCFQ
                     CGPVSIFWTPSLPCATSNTLCCNTLTSATYVVCITNSCGDIICCSYNYIAPPPILINF
                     SATCNTTLCASASGGFGPYSYQWQEKGSTPIIGTAPCLATFKPCSTYVVTVTDSKGCT
                     QSAEFTAPSITAKTCITPGNVPNGTIDLTVNCFNQCRTYLWSNGATTEDLSGLSAGTY
                     CVTVTDCDGNTVSCCFTVLLCPLWTGCIGFTTSTFTNGVYNAVPNPLPPPGCVYTYEW
                     CDINGNVIGTNRSYVVYLTCLGCPPVPVPTSLKVTNGCGVVTTINYVPITATITQTKL
                     ACCNGDGQLTLNASGGCAPLVISWERRPASGGLFVSISNNTSTVNNADTNYFYRAKVT
                     DCCGNVKYSNLLKPKGPNKKSNPTCTFSINYISSTQQILIANVAGGCGPYSYVWNVPC
                     SKIIYATTANSQSVYKCAGSAPKFYAVAITDACGRITTCSTFFREGSNGELIEASSAA
                     IAYPNPATELTNVQFINEKAEPVLIQIFNMTGKLAMEQTFQAQEGNNEAILDIKSLSK
                     GLYILQLNRNGQKETIKLAKN"
     gene            complement(55208..55954)
                     /locus_tag="PK736_00545"
     CDS             complement(55208..55954)
                     /locus_tag="PK736_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRC31909.1"
                     /translation="MIKCIIVDDEIAHIDYFKKAITSLKGEVELIGFASSVIEGIQLV
                     NQTAFDILFLDVQMPPLTGFDLLKAVANKNFEVVFTTSFDKYALDAIKFSALDYLLKP
                     YSKDDLFASFEKYKQHAQKNQTPLKVENLLHNINPNNTRKKIGLSDKNGIEFFDLNDI
                     IYCKAENVYTTFYFENKKEIVTSKPLKDYERELEHFSFFRIHNSYLINMMKVSKYIRG
                     DGGQVKMTDGTVLDVARNKKESFIQHLQEI"
     gene            complement(56089..57975)
                     /locus_tag="PK736_00550"
     CDS             complement(56089..57975)
                     /locus_tag="PK736_00550"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRC31910.1"
                     /translation="MSVQKIISNFYERWKNNVLLSLCIILVLHYQVLSGQQTEVDSLK
                     SLLVNAKNDNHKLDILNQLIELIEEDSIWLPYNLEMGQLSSSLLKKTNNAAEKKELQK
                     YFATHLYFNGIYYYDQAQLPKALNFHLQSLSYFEKLNLINKIAEAQNEIGTVYNQLGD
                     YNNALKYLQLSYNSQLKNNDSTAIAQVLNNLGAVYNMLNKKDEALFNFIQSEKLFRAI
                     GDSRQQAVLLSNIGGTYEKMHEIANALEYYKSSYAIFSQLNDAEGLYTVNIEFGNLAM
                     EDKKYADAEMYFTQAQKNADFLGTLTARLGVAKYLHQLYSLQGRYKEAYEMQSLYRVL
                     TDSLRNDENRLSIMRLQAGYEFDKKEEQLKLEQVKKEAQAQAEIQRQKVIRNSILGGA
                     SLLTISAIISFLFYKRRREAETQSKIMHLRMQAINAQMNPHFIFNCIHSIQNFISTAD
                     MQKADDYLLVFAKLIRKVLDNSTQETVTLEDDLEVLKDYVALEKLRLNFPLEFKINVD
                     ENIAVDQIYIPPLLIQPIVENAIIHGIKPLHKNGSIFINIAKGDKEIKIQVQDNGIGR
                     PKEKTATTNRKSYGIPLTKERLQHLSKAARQKSDYTTEDVYNNANESEGTRVTITIPF
                     VGED"
     gene            complement(58094..58756)
                     /locus_tag="PK736_00555"
     CDS             complement(58094..58756)
                     /locus_tag="PK736_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31911.1"
                     /translation="MKGEAKMEIDFMPLADYINKKYIVNTLNIIYDAIDNDTRPRLNI
                     IFEKFEEAEKFKKKFGFDEVKQREIATQFKMTLINQGLAIKKNIWNYFSPHNSDKYKT
                     ENIWIIFSAFEPIAKTEVTWKITKEEIQNLKSQLNMEDFWEIHKGTYGTPTFFFYNDI
                     QATQNANNEARLLLTEKYFALLKKYDEMNYFKREEFFIHIDSKENLERNYEGNWFNYD
                     RK"
     gene            complement(58847..60697)
                     /locus_tag="PK736_00560"
     CDS             complement(58847..60697)
                     /locus_tag="PK736_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HRC31912.1"
                     /translation="MIRKINSQRIIFAISITLVVTLFNACGNSVSKNEIKNMTPLLDS
                     NCTDEKITNNKLVIYQLMTRLFGNTNSSNVFYGTRAQNGCGKFDDINQKALSELKKFG
                     VSHVWFTGALEHATMSDYSEYGISQDDADVVKGRAGSPYAIKDYYDVNPDLANDVKNR
                     MAEFEALLKRTHDAGLKSIIDFVPNHVARSYGSDAKPNGVKDLGENDNKGHAFNPANN
                     FYYLLNQKFIVPADYNPMGTEKGLNENQVYDEYPAKATGNNVFSPTPSKDDWFETVKL
                     NYGVDIQGNQTKYFDPIPDTWNKMTDILLYWAAKGVDGFRCDMAEMVPVEFWNYAIGK
                     VQQKYPAVIFIAEIYNPAEYRNYINQGKFNYIYDKVGIYDALRRLIEDKGKVNDITHC
                     WKYETNGISNHLVRFLENHDEQRIASPLFGKAAKNAFGAMAVSATLGSGPLLIYFGQE
                     VGEAGAGAEGFGGDDGRTSIFDYWGVPAHQAWMNNHKYDGALLNDEQRSTRNFYNRIL
                     RLCQKSSAIQSGYFYDLQEANAEKYTNNLVYSYIRYDANEAFLISVNFSTNAYQGNIF
                     IPENVIKQISLPQETKELNDELTKDKVVYNPNEGVKLNIQPKQAAIVRLR"
     gene            complement(60705..63035)
                     /locus_tag="PK736_00565"
     CDS             complement(60705..63035)
                     /locus_tag="PK736_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HRC31913.1"
                     /translation="MKDYLKHDEWKIIEDSFHASYNKISESLFSIGNGRFGQRGMFEE
                     KYSGQTMQGSYVAGVYYPDKTRVGWWKNGYPEYFAKVLNAADWKSIHVKVNDDEVDIA
                     NCELLLFCRTLNMQTGTLTRTMQIKTKSGVVLDIESQRFCSIANDDTAVIKYSITPVN
                     DNVRIQLSLFIDGNVRNADSNYDEMFWNEVSQTASGNQLIVITETRKTNFRVATGCQW
                     IVAVDEKEVPCKTNHHTASMYAGANIETAILKGQMLTACKYVSIVTSQNYENTELQNA
                     CVNSLMRSANKGYFNLMNDNAEAWQHKWDEADIKIEGDVAAQQAIRFNIFQLNQTYTG
                     KDARLNIGPKGFTGEKYGGSTYWDTEAYCIPFYLSTASETVARNLLMYRYKHLPKAIE
                     NAAKLGFNNGAALYPMVTMNGEECHNEWEITFEEIHRNGAIAYAIFNYSRYTGDKTYL
                     YDYGLEVLIGISRFWYQRVNWSIEKNKYVMLGVTGPNEYENNVNNNWYTNLLAQWCLQ
                     YTIESLNALKKIDIVKFKFLLEKISLNETLETDKWKDVIANIYLPEHNKLGIFLQQEG
                     YMDKEQILVKDLPAENKPLNQKWSWDRILRSCFIKQADVLQGIYFFESNFTKEEIKRN
                     FDFYEPRTVHESSLSPCIHAIIAAHIGDTDKAYEMYLRTARLDLNDYNNDTEDGCHTT
                     SMAGTWMSIVEGFGGMRVKDDKLHFNPVLPVQWQSFSFKVRFRQHIIQLTINKNQIIL
                     DNITGENISVFIQNKLVTLKQDESTILNLKRKVLEQ"
     gene            complement(63056..63709)
                     /gene="pgmB"
                     /locus_tag="PK736_00570"
     CDS             complement(63056..63709)
                     /gene="pgmB"
                     /locus_tag="PK736_00570"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HRC31914.1"
                     /translation="MNINTLIFDLDGVIVDTARFHFLAWNKLANTWNHTITHEQNEEL
                     KGINRMRSLDLIAGWCNVKLNDNQKKEFAELKNNWYLEQVKQMTESDTLPGVKNLLTE
                     AKNNNIKIAVASASKNANEVLRNINLIQEFDYIADGNSVKESKPNPAVFLNVAQHLKV
                     NPAQCIVFEDAPAGVEAALAGGFHVVGIGNKQSLPNAHFILAGFENISLNDILNFFK"
     gene            complement(63718..65679)
                     /locus_tag="PK736_00575"
     CDS             complement(63718..65679)
                     /locus_tag="PK736_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRC31915.1"
                     /translation="MTVNYLINGANFFSIINKIMRPGCIVANIFYKYFFILLVTSLFA
                     NTNLLSQNKTAKIAIDRIDPPHWWTGFINQNLQLCVHGNNISATTPAINYSGVTITNV
                     IRPENKNYLFIDLKIDESAKPGVMNIQFTGGKKNITFPYLIKQRNISSAGKKGLNTSD
                     AIYLMMPDRFANGDTKNDVVPTMNCKDVNRDSLIMRHGGDLQGVMQHLNYIKDLGFNA
                     LWLNPVVENNQPTESYHGYAFTDQYKIDARLGTNSLYKQLVDTCHKLGMKMIYDIVLN
                     HIGNQHYLIKDLPANDFVHQWNSFTRTTYKDGTLMDIYAADADKKQMTDGWFDVHMPD
                     LNQQNPLVENYLTQNAIWLIEEFDIDAFRIDTYAYSDLDFESRWAQKIKTEYPQFFMF
                     AETWVHGITNQSFFTKNNITSSLHKSNIDGVTDFQLYYATNDALNQPFGWTEGVNRWY
                     HALCADYVYQNPNNNILFVDNHDLSRYWSVCGKSLDKYKMGLIFMATTRGIPSMYYGT
                     EILMENFSNGPSSNVRNDFPGGWPGDKINYFEAKNLPDTIKQAHEFITKLFNFRKNNP
                     TIYQGNLKQFVPVDGIYVYFRFNDDQTFMIVHNSNNTAKELSLNRFEEMIPDTVYAKD
                     VMTGKVTQQQGKISLEKNQSVIFQIMKTK"
     gene            complement(66096..67397)
                     /locus_tag="PK736_00580"
     CDS             complement(66096..67397)
                     /locus_tag="PK736_00580"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRC31916.1"
                     /translation="MYKNISATILFIFITIIFIFQSAKTYGQALPQKCATEMYMQQLY
                     AKNPQLKKSLIDAANNETNIQRTQAIITIPVVVHVLHRGEPIGTNTHITPQQIESQIT
                     ILNNDFRAQNDDITNVPIVFQSKIGDTKIQFCLATLDPDSNPTSGIVYKQTSQPYFSL
                     DNNVKFDTSGGSNAWPADQYLNMWVCDLRNFNGDNLLGFAQFPNGPAETDGIVVRYEA
                     FGNVGNVSLPYNKGRTTTHEVGHWLNLIHIWGDNVCGNDLVSDTPTQEQANFGNVVNG
                     AYLCPGFPHVTCNNGPDGDMYMNYMDYTFDKCMHMFTKGQSNRMNKAIVDYRASFLTT
                     KDLCVPPNISPDTNVVNFELVSNNTLLNSINILVIGNLSTDVEVAVYNTLGQSVLSAT
                     YAKSETATAMKIDLPNLKTGFYYISIFANSKQSKLKFYKLQ"
     gene            complement(67723..71325)
                     /gene="dnaE"
                     /locus_tag="PK736_00585"
     CDS             complement(67723..71325)
                     /gene="dnaE"
                     /locus_tag="PK736_00585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRC31917.1"
                     /translation="MPTFNHLHVHTQFSLLDGAASIGKLFKKATGDKMRAIAITDHGN
                     MFGAFEFVAEAAKYNKENYDVIKPIVGCEFYVVDDRTRKQFTKDDKDVRYHQLFLAKN
                     AEGYKNLVKLCSLGYIEGMYGKYPRIDKSLILQYHKGLIATTCCLGAEIPRTILRHGE
                     DEGEKLFKWWLDLFGEDYYIEIQRHGIPDQNKVNEVLLKWSVKYNVKVICSNDSHYVN
                     QEDANAHDILLCVNTGEKQSTPKMKDFGDDDTFIKGARFAFYNDEFYFKTTEQMLHTF
                     SDIPYALDNTEHIVDKCDILNLKKDILLPFYQIPDGFLTQDQYIRHLTFEGAKNRYHE
                     LTTEIEERLNFELSVIESMGFAGYFLIVADFIKAGRDMGVFVGPGRGSAAGSAVAYCI
                     GITNIDPIKYDLLFERFLNPKRKSMPDIDTDFDDEGRQKVIDYVVQKYGRNQVAQIIT
                     YGTMAARSSIKDVARVLDLPLNESNLLAKMVPEKPGTELNRILLGPMDGEKGIKEHDG
                     FNSEALEDIKKLRDIMAGNDIRADVLKEAVKLEGSVRSTGVHAAGIIIAPCDLTDIIP
                     VSVAKDSDLWVSQFEGTKIEDAGVIKMDFLGLKTLTIIRDALQLIKQNYGIVIDIDTI
                     PLDDKATFDLYQRGDTNGTFQFESVGMQKYLKELKPDKLEDLIAMNALYRPGPIEYIP
                     SFIRRKHKQEPVTYDLPEMQEYLEETYGITVYQEQVMLLSQKLGGFDKGDADNLRKAM
                     GKKNIKDLNKMKGQFMEGAGLRGHNLITCEKIWTDWEAFASYAFNKSHSTCYAFVAYQ
                     TAYLKANYGPEYMAAVLTHNLSNIEKVTFFMEECRRMNIQVLGPDVNESSFKFTVNKQ
                     KQIRFGLGAVKGVGENAVDSIIENRNKGGNYLSIYDLAKRVNLRSVNKRTLEGLAKSG
                     AFDSFKNIHRAQYLTAGKDELTGIDYAVKYGQQQQEAENASQISLFGDVGSKSDSTEP
                     RLPTSEPWSKLEQLHHEREVIGFYLSGHPLDLFKYEMKLFCTHKCSDLQPDELKNLYK
                     KDFSFAGIITKANHRIGKTGKRFGIFTIEDLTSAVEFPLFDEKKYLEFEKYLKEQMFV
                     FVRATVQNKYNKPEEFELKINNIQLLGDVGDSNCRGIVVHGKSHEINGAWIDDLLQSV
                     IAKPGKVPLRIAVKDDESTYYVDATSRKYKLAFDRELFEEWEYKGYDFKIEKSG"
BASE COUNT        21832 a        13416 c        13738 g        23252 t
ORIGIN      
        1 tgcatttaca agaactgtat gtgaaatgct acaaccgatg gcatctgtta ctgtatgtat
       61 ttatattcct gcaggaacgt atactgttac tgttacggat gataacaact gtttttcggt
      121 tatctcgcaa ttaatttcac aacccgattc tatagaaata actaccacca ttgtgcctac
      181 taagtgtaca gataataatg gttctataac cgcttcggca attggcggtg taggttcatt
      241 aagcttctta tggtctaccg catctacagc acaaacaatt tcaaatttag tttcgggtac
      301 atatacagta accgtcaccg accaaaatgc ttgtactgtc agcaaagtag tagtagttcc
      361 gtttttatct cccccacaat tgagtatagg aagttttact aacgttttat gttttggtgc
      421 tgctaatggt gttataaatg tgaatatagg gaatggtaat cctccgttta cttataattg
      481 gagtaatggt gcaactacag gatctaccat ttcgaattta gctcctggaa cttacacttt
      541 gatactaact gactctgttg gttgtatcga ttctatttcg caagatataa ctcaaccaac
      601 agcgattcag gtaacattgg ctattacaaa tctatcgtgt ggttttcaaa atggtgctat
      661 tgcagcatca gcttctggtg gtacaggtac attaacttat ttatggtcga atggtgcaac
      721 cacctcaaca atcaatggct tatctgcaat tacgtatacg gtaactgtta ctgatcaaaa
      781 caactgcaca aaaacagcgt cttctacttt gattgcattg ccttttccgg ttgccaattt
      841 gataacatcg ggtaatgtca gttgttttgg aggaaataat ggatttattt ctattggtat
      901 ttcgggcgga actccacctt actctatctt atggtcgaat ggtgatacta caattaataa
      961 catcaatctt acttctggta cctatacagt cgcagtaacc gatgatgatg gttgtactgc
     1021 aactttaagc cataccataa cagagccatc actgctcgaa gtaagtttaa cttttacaaa
     1081 tgctatttgt ggtaacaata atggaacaat tcaatctgtt acaacaggcg gggtaaatcc
     1141 gtattcctat ttatggagta atagcgccac tacaaaaaat gttaataatc tagctcctgg
     1201 tttatataca ataactgtaa ctgattcaaa ttcttgcact gccactgctt ccgttaatat
     1261 agtgcaggta aacgtccctg ttatacaact tgacagcgta aaaaatgcct tatgtaaaaa
     1321 cagcctcgat ggaggaatct tcatttcggt tgcgggtgca ggaccatttg tttatttgtg
     1381 gtctaatggt ttttcaacac aggatataaa tggggtaggt gcaggcactt acaatgttac
     1441 tgttaccgat acaaactcat gccaatctaa ttttacgcaa attattactg aaccaaacgc
     1501 tattgttctt tcaatatcta aaaacgatgt gacttgcaat agtaacaatg gaaatgcagt
     1561 atgtaatgct accggaggaa caggaacatt tacctttgtt tggtctaatt ccagcacaaa
     1621 tgattctatt ttcaatttag ctcccggtac gtatactgtt actgttaccg acataaataa
     1681 ttgcacagcc actcaaagcg taaccatcaa ttcaaccttg ttgcctgtaa tcactgtgtc
     1741 aaacatcatt aatgttaatt gtaaaggcgg agataatggg cttattactg tgacagttgc
     1801 aggtggagtg cctccatatg tttattcatg gtcgaatggc aatacaactg cggaaataga
     1861 tacgcttaca gcaggcttat acattcttac tgtaacagac ttcagcggat gctcggttat
     1921 cgattccatt gtgattactg aacctgattt attgcaattg agtctgaatt cttatcctgt
     1981 gtcatgtttg gcaattgacg gagccataaa atgtaatgtt ttaggtggaa cagcacctta
     2041 cacttattta tggagtaata gtgcaactac cgattctatt tttggtttaa atgtaggaac
     2101 gtatactgtt actgttattg ataataaagg atgtgttgca acattatcgg ggcaagtggt
     2161 taaagctcct ggcccacaaa tttcgcttaa taaccttgta ggaataggat gcagtggttt
     2221 accggtcggc tctattgaca tttcagtaag tggtggaaca atttttacat atctgtggtc
     2281 gaatggtgca tccactcagg acattaatac cttattgcca aatacttaca ccgttactgt
     2341 gacaaacgag tttggttgta cttcggttga tagttttatt gtaacaggct acttcccaat
     2401 agttttaaac acttcaatta ctaatgagac atgtggtgca ggaaatggaa ctgcaactgt
     2461 aactgctaca ggtggaaatg gtgtttacac ttatttatgg tctaatggac aaacaacctc
     2521 cactgccaca ggcttggttg caaatacata tacagtttta gtaagtgata ccggtgtatg
     2581 ttccaattcg aaaagtgtaa ccattattaa tcttgcaaat ccagttgtta caaatgattc
     2641 tataataaag acattatgca gcggttcaat tgatggtgct atttatattt cggtaagtgg
     2701 taatggcccc tttacttatt tgtggactgg tggaagtact ttgcaggatt tgattaatgt
     2761 agcttctggt tcgtatactg ttacagttac tgatacaaat tcatgtgtaa caactgctaa
     2821 ctttacaatt gccccaacgc tacctctcaa cttgaatatg aatgcagtaa gtgctagctg
     2881 tggagttgcc aatggttatg cctttgtaca ggtttcattg ggcactgcac cttatactta
     2941 tttatggact ggtggcgcca caaacgatac catttctaac cttgttgcag ctacttattc
     3001 agttacagtt accgacaaga acggctgttc agataccgct tcggttgtag ttaatgcatc
     3061 aaatcaaatt ttgtataatg tagattcggt gataaatgtt aattgtaatg gcgacagcac
     3121 cggagcaatt tatgtgaatg tagtaaatgc ttctgtgttc tcatatcaat ggtccaatgg
     3181 tgctactacc tccaatttat taaatgttcc agcaggaatt tatactctgt ccatcaccgg
     3241 accaaatggg tgtttcgctg atacaacctt aacaataagc caacctcttc ccattatttc
     3301 aaatattaca tccacccctg caggctgcac acttgcatat ggtacggcaa cagtaaatcc
     3361 aaccggtggc aatggcactt atacatattt atggtcgagc ggtagcactt tgaacacagc
     3421 cgtaaattta ctttcgggat atgtgagcgt agccatttct gatgttaagg gatgtacttt
     3481 gaaagattca gtatatgtgg gaatttcttc acagattact gttacatccg attcgttgat
     3541 taacccaaaa tgcgctggtg gaaatgatgg tgccatatac atatctgtta ataatggcat
     3601 tttaccgtac acatttattt ggagtaatgg tgtaacttcc gaagataatc ttaatatacc
     3661 gggaggtgcg tatacagtta tagtaacaga tagtgttggc tgtgccgata ctgtttcgta
     3721 taatcttttt gagcctaatg cgcttagcct gggtttttca tccactccgc caagttgtat
     3781 tgccaataat ggtagcatca ctgtttctgt ttcgggcggt accccgctat atacttactt
     3841 atggtcgaat ggtgcaaccg atgattctat attcaactta tcgccatcat ggtatactgt
     3901 tacagttaca gatgcaaatg gttgtacaat ccaggactct ttattgttga gcattccggg
     3961 ggcacctgta ctttcaattg attcagttac caatgttata tgcggagggg cgggtaatgg
     4021 agcaatttat attactacta ccggaggcac ttcgccattt aactatttgt ggagcaatgg
     4081 agccacagat gaagatcttc tctctgttgc cggtggtaca tatacagtta acgttaccga
     4141 tgcaaacggt tgtagctttt ctttatctgc cacaatcagt gaaaatactg ttttgcaagc
     4201 tcaagtgttc acgcaaaatg ccggatgcaa ttctaacaat ggatttgcat atgtagttac
     4261 aagcggtggc actcctcctt atacaattaa ctggcttttt aatggttcca gtcaagacac
     4321 tatcgcttca ttgtttccgg gttcttatga tgttgttgta acagatagta atggatgtac
     4381 tacctcagca acaggtatag ttaataattc aaatgccgca tccatcattc cagattccat
     4441 tgtaggtgta tcctgctttg gattaaacga tggcgccata tatatatcga ccattggagg
     4501 tgttgcccca ttcaactatt tatggaataa tggaaataca aacgaagata ttacaggctt
     4561 agctcagggt atttacacca taactgtgac agatatatta gggtgtataa caattttttc
     4621 ggatactgtg acgcaacctg attcgataca atttgttttc acagtggatt cggccagttg
     4681 taatattgca aatggatcgg ccagtctagt ggtttctggc ggcacatcgc catatacgta
     4741 taactggagc aatggatcgc aaactcaatc tataaacgga gtgcttgctg gcatctatac
     4801 agtcactgta accgatgcac atggttgcag tctttcaagt aatgtaattg tacccaatta
     4861 ttcttcgcca tacataagtt catcttcagt aacaggcctt acttgttatg gatcaggtga
     4921 tggattcatt gatatcacta tatccggagg ctcaccacca ttctcttttc tttggaatga
     4981 tggcaataca aatgaagatc gttccactct ttctgcaggt acgtatacag taacagtcac
     5041 cgacattcat aattgtactg cattacaggt atttgttgta agcgaacctt ctgatattgt
     5101 tttttccatt caggtgcagg atgcaaattg taatacaaat gatggcagcg caacagtaac
     5161 aggtttattt ggtggggttg gcccttatac tttcttatgg tccaattctg ttactatttc
     5221 aacaataagc aatgtgtttg ccggaacgta tactataaca gttacagacg caaagaattg
     5281 caccaaaaca gcaattgcca gtattggaaa tatttcgggt cctgtgatta ctgttgacag
     5341 tatatcagac attctttgtt ttggcggatc caatggttac atttcggtaa atgtatcggg
     5401 aggcaatccg ggttatacat ttttatggtc gaacggagga acaacaaaca caatttcgaa
     5461 tttgacggca ggcacctata atcttgctgt taccgatata ataggttgta aagattttta
     5521 ttctgtcaat tttctagagc ctgctgagat taatttgaac cctcttttag caatcaataa
     5581 tccaccgtta ttcaatatca gttgtaatgg ggccggtgat ggaagcattg ttcttaatgt
     5641 aacagggggt acttttccat acatttattc ctggacaaat ggtgcatcaa catcggcaat
     5701 ttacaacctc tcagcaaccc tgtaccaggt gcaggttacc gatgcaaatg gttgtttaaa
     5761 gaaagacacc tttttactca cagaaccacc tccattgatg tcggttggtg gaagtgatat
     5821 tgaaatttgc ggtgaggatt cagtacacct caatgcaact gcaccaactt atggttacgg
     5881 attttggtcc gtgttaaatg gaacgggagt attcgatgat gacaaatcac cgcaggcatt
     5941 tgtgtcaggt ttgtcgtttg gggttaatac ttttatgtgg attgttgccg acagttcctc
     6001 ctgtaaagat acttcattgg taatagtaaa cgtgcttgaa caaatagatg ccatagccgg
     6061 aagcgataga gagatttgtg atgatcaggt agtgttgtct gcaaccgccc ctcaatttgg
     6121 ttttggatta tggagcacat ttaacagcag tggaataatt caggatacta cacaagcact
     6181 tacaaatgta tttgtgctca atgttggttc aaatgttttt caatggactg tttacaatgg
     6241 cggatgtaaa gattcggatt tggtaaatat tattcgcctc gattctcttg actgtctcac
     6301 accaatagat ttgccaacag gtttttctcc taatggtgat ggtaccaatg ataattatgt
     6361 ggtgaaggga atactggatt atcctgataa tattttgatt gtttataaca gatggggtaa
     6421 tattgtttat caaaaaacaa gttatgccaa tgaatggaag ggtacaaata ataatggcga
     6481 aaacttaccg gagggtacct attatgtaac cataaaaata aaaggcttta caaagtatgt
     6541 aaatagtttt atagacttac gtagataaaa acatgatgat gaaaaaaatt atatttctgt
     6601 ttttttattt ctgtttgcaa agtgggatgt atgcacagca ggatcctctt ttctctcaat
     6661 acatttttaa tggacttgtt attaatcctg cttatgccgg atcgcatccc tatatgacac
     6721 ttaatgtttt agcgcgcaag caatggtttg gaatggacaa gtcgcccagc actcaagcta
     6781 tctcctggaa tggcccattg aaatcaaaga aggccggttt aggattaata ttaaacaatg
     6841 atagagcagg aattatacgg caaacagatt tattcggaag ctatagttat cacctttcat
     6901 taccaaaagg aaaattaaat ttcggactaa gtggtggatt aagtttttat cgtagttttt
     6961 tgtcggatgc tgttatctgg gaagcgggtg atgctgtgta tgccgccaat tcaatatcga
     7021 atacctatcc caattttggt gctggggctt attatcacaa cgaaaattat tatttcggaa
     7081 tttcgatgcc tcgtgtatta agttattaca gccagttgaa acaaggcgat gaccctacta
     7141 ctgtgcaccg acaaaaagct catttttatg ttactggcgg ctacatgata gaaacaaaat
     7201 ccgacattaa aataaaacct tctgcactaa taaaatatgt aaaagctgcg cccattcaat
     7261 tcgatattaa tttgaatctg ttgttcaaca atattgtgtg ggctggcctt tctttccgct
     7321 caggcgatgc tatttctctt aacttagagt atcaactcac ccgtaagttg cgtgttgggt
     7381 atgcttatga ttatactatt tcgaggctgc gttattccac ttcaggaagc cacgaaatat
     7441 gtattggtta cgattttgga tatgatatta tgaaaatgaa aaaccctcgt tacttttaac
     7501 cccgaataac aagtaaaatg ctctcagcac acaattataa attctttatt ttaatatttc
     7561 ttatagcaat attgttcagt tcctgcgcta atattcgata ccgcaccggt aatgcatatt
     7621 atgacgaatt gttgtatgcc gatgccgcca aaaaatatga aatgattggc aacaagagta
     7681 aatatgagga tgtaatattt aaacttggcg actgctaccg gcttacagga aataacaaca
     7741 aggctttatc ggtttatcaa agaattgtgg tacaaaaaaa ttgtaaacct atcgatcgat
     7801 tttattatgc cgaagcactc atgaaagccg gccgatataa ggatgcaaaa aattggttcg
     7861 attcatatct taaaaacacc aacataaacg atacccgtgc gcaacaacta tataaggcat
     7921 gcgattcgct caaagtattg tataaagatt ctgcattgta tgtgattcaa cttatgccgt
     7981 tcaatgcaaa taatgtttcg agttacagcc cggctttttt taaagatggg ttagtatttg
     8041 tgagtgatag aagtgcaccc acatcgaaaa attatcgctc gggatggaca ggccaaaaat
     8101 atgtggatct gttttacact gagaatctcg agggtgataa ttggtcgatg ccacaacctt
     8161 ttgccgatgg cataaattca ccatacaatg aagggccatg catattcaat aaagacaaca
     8221 acataattta ctttacacga aatagtcttg atagaggcaa gccaacagta aatgccaaat
     8281 tggagaataa tttaaaaatt tataaagggg ttttggacaa caatacctgg aaaaccattg
     8341 gagagttgtc cttcaacaat actaattatt cggtttttca tccagctata gcgcaacaag
     8401 ggcaatcgat gtattttgct tctgatatgc cttggggtta tggaggtaca gacatttaca
     8461 gagtaaagtt tatcaatggc cgttggtcga aacccgaaaa tttaggacct gaagtgaaca
     8521 ccatgggcaa cgaagcattc ccgtttattg ttggcgatag tattttgttt tttgcttcgg
     8581 atggtaaatt tggttttggt gggtacgaca tttttgaagc cgaaattgaa aatgacaaat
     8641 gggtgcatgc agttaacctt ggaagtcccg tcaactcttc ggcagatgac ttcggattga
     8701 taattcaaaa agggggcgac aagggttact tcgcatcgaa ccgcaacagc aaaactgaca
     8761 gaatatattc tttcaaaaaa gcgccacctc aggtttcgat aattggcaca gtcaccaatt
     8821 ttcctgaaaa cgaattaata ccaaattgca aagtgaaaat aacttctaca aataatgtag
     8881 atactgtgat tgatgctgat tcaaaaggac aatttaaatt cggtttggat cttaatcaga
     8941 gttataaatt tttggtttcg gataaggcat attatgctgc cggatttgaa gtgcaaactt
     9001 atggtaagaa gtcttccgaa attcaggaaa ttcctgttgc tctggatcat attattttaa
     9061 agaaaagtaa aatatactac aagctaaaat ttgaaacaaa aaccacggtg tttaaaaaaa
     9121 caacgcaacc ggcccttcag gaattagcct tagtgatgaa acaaaatccg caaataaaga
     9181 ttgaaatagt ttcgcacacc gattcgcgag gcgatgataa accaaatatg accctcacgc
     9241 ttagcagagc caattcgcta aaagatgcgc tggtattaat gggtattgat ggcaacagaa
     9301 ttgccacaaa aggaatggga gaaagtgtga ttttaaatca ttgcaaaaat ggtaaagtgt
     9361 gtattgaaga agagcacgag gataataacc gtgtggagat aactgttacc tctatcgaca
     9421 attagccaat aacaaaacgt ttttttcgaa atctgtattc tggttcgtat accgttgcag
     9481 ttagaagtga atccattcaa tctataactc ctctaattta tttataaagg aagtgtataa
     9541 atgccaggaa attttatttc atgagcagtt attaaatcga caatgggttt aaatgagttt
     9601 acgctattgt attatcaatt aaaaaaaagc atcgcaaatg ttgatggaca aatcaataat
     9661 ggtgtcaaat aaaatgagtc aaaaaaaaat ctgcctttcg acagatttat ttttgggtgg
     9721 gagatggggc tcgaacccac gaccctcaga atcacaatct gatgctctaa ccaactgagc
     9781 tacaaccacc ataattacaa gccgcgaaag taatagtatt tatgcaatta ttaaaaccat
     9841 ttttttttat ttttaattaa aaaatatttg ttccatgatc ctttaaattt acatttggaa
     9901 tctcaaaatc aattaaaaca ttttaaaatt tattcactat gaagaaaaaa attctgttca
     9961 atgttgcttt attggcgata atggccacag cacaaaatgc aaatgctcaa tggagcaccg
    10021 caggtaatgt acttgttggt accgaaaaac tcggatctac aaatgcacaa ccggtaaaca
    10081 tctttaccgg aaatttaaat aggatgacga tagccgcaaa tggaaaagtt ggaattggaa
    10141 caactacacc ggtaaataac cttgatatat ttagcaccgg agccgtaact tttggaatta
    10201 gatcaagtac cggaggtgct accatgtcga tggacaaatc tactattgca tcaaatgctg
    10261 cattcgcata caaatatcta ggtgttccta tttggaatag tggatgcttg ggtaacaatg
    10321 attttagcat tagaaatgtg gtgactaaca ggttctccat gataatacgt gcaaatgaca
    10381 atgtaggtat aggtcaaaat aatcccgttg ctcgcttaga tgtgcgttcg ggtagtggcg
    10441 tgggcgatac cattcctgca ataaatgcta ccattggata tacgggcttg tatgatttgc
    10501 cggcagtgca agcaacatct gaaccaagcg ctggatatgg cattggagta gaagctacag
    10561 gcggttggat tggtgcacgt ggttttggcg gcaccaccgg agttgttggc gtcagtggtt
    10621 tgaatggagt atttggaata gctatagatc aaactggtgt tgagaatatt acaggagttt
    10681 atggagctgc agatggcggt gatgtatcgg taggagttta tggtaactcg cagaacgcaa
    10741 caaataatta tggtgtttat ggtgaacaaa cagacactgc tgctgcagat tatgccgtag
    10801 tttgtttggg cgatttattt tattggcgcg catttgcacc atccgatgca cgtttaaaaa
    10861 ataatattca gccttatgca ggcgcattgt caaaaatcgg aatgttaaat acttctactt
    10921 acactttcga ccatgcaaaa tacccaggca tggctttacc gggaggtaaa caaataggtt
    10981 ttatggctga tcaacttgaa aaggtatttc ctgaaatggt aaaaaattct gatttgccag
    11041 caggtgcaac acgcgattca agaggtaaat taacgtataa aactatcaaa gatgtaaaaa
    11101 cggtaaacta cattagtttg atacctgtac ttgtaagtgc agtaaatgaa caacaagcca
    11161 ttattgaaaa taaaaatgct gaaatggctc aattgaaaaa tgaaatggca gagttgcgtc
    11221 aacagatgaa tgaaataaag cagatggtgc aaaccgctga tcgcaaatca aattcatcaa
    11281 cattgggatt gaataatgct acaccaaatc ctgcaagtgg cttagttgct tttagcgcta
    11341 atgttcctgc cgatatgaat aatgccgaaa tacaattagt taattctaat ggacaaattg
    11401 tacgcactgt tgttctttca ggtaagaatg gtttgttaag ctcaacattg aatacagcag
    11461 atctggctaa tggcacttat acttatacct tgtatgttgc cggcaagcaa gtagaatcta
    11521 aacaattatt gataagcaaa taatttcaat ttttgaattt taaaaaaagc ccggttgatt
    11581 ttcagccggg ctttttttat tatctttggc tttgcaatcg tatactatta tagttaacat
    11641 ttttatattt taaataaacc ggtgatgaag caattttttt ctactgtatt aattgtcctt
    11701 tgttgcatat gctctaatct tgcacagcaa aaagaaaaca cacttgaaat cgaaaccaaa
    11761 tttatagaat cgttgcaatg gcgatgtata gggccataca gaggtggacg cagttgtgct
    11821 gttgccggtg atgaaaaaaa taaacaaaca ttttatttcg gcagcactgg tggcggtgtt
    11881 tggaaaacaa ccgatgcagg taataactgg aaaaatattt ccgataaata ttttggcggc
    11941 agcattggtg ctattgcagt aagtaaaagc gatggagata taatatttgt tggacagggt
    12001 gagcaaactg ttcgtggtaa tgtgagcgaa ggaaatggaa tttggaaaac cacagatggc
    12061 ggacgtacat ggcaatttct cgggcttaaa gatacccgac acattgtccg tatacgcatt
    12121 catcccaaaa accctgatat tgtttacgtt gcagctttag gacatttgtt tggcagcaat
    12181 gatgaacgag gaatttataa aaccctggat ggtggcaaaa cctggaaaaa aatcctcttt
    12241 attgataatc aaacaggcgc tgccgatttg gtaacagatc ctttgaattt tgaaactatt
    12301 tacgccacca tgtggaatat taaaagaaca ccatattcac ttgaaagtgg tggaccaaat
    12361 tcatccattt ggaagagtgt ggacggtggc aatacatgga gaaatataac gaataacgat
    12421 ggattaccca gcgaaacatt gggcctcata tgtgtaacgg tttcacctgt caatcccgac
    12481 cgggtatggg caatgataga agcaaaaaat gggggcttgt tccgcagcga tgatgcggga
    12541 gaaacatgga ccaccgtaaa tcaggataga aagttgcggc aacgggcgtg gtatttttcg
    12601 cgaatttatg ccgaaccaaa agatgccaat aaaattattg cccttaatgt tcaaatgttt
    12661 cggtcaacgg atggtggcaa atcgtttaat aatcaaatta ataccccaca tggcgatcac
    12721 cacgatttgt ggatagaccc tgaggatgcc aagcgcatga ttgtaggtga tgacggagga
    12781 gcacaaatat caaatgatgg cggaattaca tggtccacat atcaaaatca accaacagca
    12841 cagttttatc gtgttactac cgacaaccat tttccttacc gcatttatgg tgctcagcag
    12901 gataattcat cggtgcggat tctgcaccga tcttccaaag gaagtatagg ccgcgatgat
    12961 tggcaaccaa cagccggtag cgaaagcggg cacatagtag ccgacccttt gaatgatgac
    13021 atcgtatatg gtggcagtta tggtggatat cttacaaggt ataatcacaa aaccggagag
    13081 aatagaaaca ttaccatatg gcccgataat cccatgggat ggggagctga tagtttaaaa
    13141 tatcggtttc aatggaattt cccaatcttc ttttcgccca acaatcccaa aaaattatat
    13201 gccgctgcca atgtattgtt ttcaactatc gatgaaggcc atagctggca agtaatttcg
    13261 cccgacctta cacgaaacga taaatcgaaa caaaaatcct cgggaggtat tattactcag
    13321 gacaatacaa gtgtggaata ttactctacc atttttgccg cttgcgaatc gccttacgaa
    13381 aaggatttgc tttggtgtgg taccgatgat gggctcattc aaatatcccg caatggaggc
    13441 ggtgattgga aaaatgttac accaaaagat ttgcccgaat ggagcatggt gaataaaatt
    13501 gaacccgatc catttcacaa aggtggatgc tatatagccg caacaaaata taagagtgac
    13561 gattttcagc ctttaatttt taaaactgaa gattatggtg ttacctggaa aaagataaca
    13621 agtggcattg caccggatca ttttacaaga gccgtatgtt gtgatacaaa aatacaaggg
    13681 ctattatatg ctggcaccga aaatggcatg tacatttcaa ctaatgatgg aaaattatgg
    13741 aagcccttca atcagaattt gcctcaggta ccaattaccg atttaacaat aaaagatgac
    13801 gatctcattg ttgccactca gggtagaagc ttttggatat tggatgactt aacccgcatt
    13861 cattattttt tgcaaaataa agaacagact aataaagagt tctttctcat aaaaccgcgc
    13921 gatacatggc gtttaaattc gtacgtaatt acaagtagca ccgaaggtga aaatcccttg
    13981 ccaggtgtgg tagttaattt ttacatcgat aaaaaaaata aaaagaagca agccgagtta
    14041 tctttctacg attcggaaat gaaattgata cggtctttta aatcagatac aaaatcagaa
    14101 aatgaaaagc tgacaataaa aaatggcatg aaccatttct cgtggaatat gcaatatcct
    14161 gatgctgaaa aatttgatgg tatgattcta tggagtggag gtaccaatgg accttatgcg
    14221 ccacccggaa attatactgt tgtattaaag tttggaaatg attcggcagc acaaaatttt
    14281 agaattaagc atttacctaa tttggagtct acagagtcag acattaaaca acagtttgat
    14341 tttttaataa aatgtcgcaa taaattgagc gaggtgcaca aagccatttc agacataaga
    14401 ggggtaaaaa aaatattgaa taatttaacg gaacaaaatg actctttgct ttcagaggaa
    14461 tgcaaggaca gcattcaaat tgcgctcaaa gaattaattt caatagagga ggaattatat
    14521 cagacaaaat catcgagcag tcaggatcca ataaattttc cgataaaact gaacgataag
    14581 ttagctggac ttatgtcggt agcagaggag agcgaatcga aaccttcagt tcaaagtctg
    14641 gaattgttta atgtccttga taagtcaata caaattcaac tgaccagatt atcgaaagtt
    14701 tttgagcaaa caatacccga aataaacaaa atggtaagaa atttgaacat cccatatata
    14761 caaacgaaat ggtgaaaggg aaacggcaaa aaataatgct tcgataaact gcactttttt
    14821 tatagctttg cccatcaaat taaattttat gttgcgatat ttagctttgt ttatttcggg
    14881 agttgttttg tttagtgggt gtagcaaaac catcaaccca agcattatgt ttattactcc
    14941 agatgatttt ccatatactg ttgatcaaac aataggaaat atagaatatc gcatatcacc
    15001 ttatgatata atagagttta gcatttacag taacgatggc gaaaagttgc tcgaaagatc
    15061 tgtggcggca aattcaaata acgctgctgc aggattagga aataatccca taaccgaagt
    15121 tggtattaag tatgctgttg atatggaagg ttttgtaaag ttgccacaac ttggtaaaac
    15181 aaaattgatg gatatgacca ttcgggatgc tgagaaattt cttgaagaaa aatattcaca
    15241 atttataaag aaaccttttg ctatactacg agttacaaac cgtagggtaa tagtttttac
    15301 aggttcgggt gggttaggca gtgtggttaa tttaacaaat gaaaacacca ctttgataga
    15361 agcattagca cttgcaggtg gtattaccac caccggcaaa gcccgtaaaa taaaacttat
    15421 acgaggcgat acccgtaacc ctcaggtaca gcttattgac cttagtactg tagaaggaat
    15481 gaaacaaagc aacctgcttc tacaagcaaa tgatattatt tacgttgagc cggttccacg
    15541 tatctcccaa ggggtacttg ccgaaatact tccaattgtg agcattatca ccagtggatt
    15601 attaatttac aattttgttc aacaaatttc aaaataatgg ccaaaaagaa aattacagcc
    15661 ttcaatgaag atttcgattt taaacttttt tcaactattg cgcgtaaaaa ttttatttgg
    15721 gttgttgttt ttattggaac ttctttgatt ttgggtttat tatatctgcg ctacacacag
    15781 cccatatatg aaagcgcttc tgtgataaaa ttgtcgagcg aaaacaaagc taatactgta
    15841 ctcgacatta gtaaaaatta ttttaatgaa cagcagcgca atcaaatggc gagtgatata
    15901 actttgataa aatcaaagat tatttcggaa cgtgctttgt caaaactacc tcttgatatc
    15961 agttatttta taagaggtac tgttcttgat tttgaaatgt atcggtcatc accctttcag
    16021 gttcaatatg aaattttaga ttcggccatg gttggaactc gtttcgaact ggaatttgta
    16081 aacgctttaa gctttcaatt gttttgtgtt ttaccaaatg gaacaaaaat ttcaaaagaa
    16141 tttcctacag taaaatggat agaagttcct ggactaagat taaaagtatt accacttgat
    16201 tttaaaagaa ttgaaaccga gcaagctgat gttaaaacca ataaggctta ttactttgtt
    16261 ataaatgatt taaaaaatgt agcacaaagt ttttcctctc aacttcaggt caatgtgctc
    16321 aatcccgaag ctaaaacggt acagataaaa atacaggatt tttcgccaaa caaggctgcc
    16381 gatgttgtta atgcaattgc aaacgagttt atttcctatg atgtagagcg aaatgctgcc
    16441 gttgcaaaca agattatcga atttattaat tccacaattt tcaatgtaga atccttgctc
    16501 agcaacagtg aagattcact tgaacgattc aaatcagaaa caaaagtatt taatcctaca
    16561 agcagtgccg gtatactttc agggcagatt attgccttgc aggaaaagaa atttcaactc
    16621 gatctggagt ttaccatgta cgagaatttg aaaaaagatg tggtagctaa taaagatccc
    16681 ggcaacctca tattagcact tgcaggcgcc aacgatgaac aaggaataac cagcatgctt
    16741 accaacctga tgcagttaat gaatgagcgg gaaaaaatac ttattcaaac cactaccgaa
    16801 aatgctcaat accgaattat agagtcgcaa ataaactcta ctatcaacat gattattcga
    16861 tcgattaata atcagcaaga tgcattgaat acaaaaaggg ccgcaagcga ccgccaactt
    16921 aaagaattag aaaacaaata cagcctcata cccggtcagg aggcccgata tggcaggctg
    16981 gaaagattat tcaacattaa tgaaaaattt tattctttat tactagaaaa aaaggcagag
    17041 ttttcaatta cacgggctgg ttatgttgcc gaaaatattt tacttgaaca agccatagcg
    17101 cccacaacac ccatatcacc gaaaagacct ctcatattaa gttcggcact tatgctgggt
    17161 ttgctaagca gctttatcct gattgtttct cgttatttga tgcacaattt aatttcatcg
    17221 ctcgatgaag taggtcagta taccgatgca gcattattag gaattatacc aaagtacaaa
    17281 cgcgaaattc ccatttcgca attgcttgtc gacaaaaatc ctaagtctat tatttcggaa
    17341 gcgtttagaa gtattcgtac caatttgcaa tttataaatg ccgaaaccgg tagcaaggtg
    17401 atggccgtta catctacaat ttctggcgaa ggaaaaacct tcaatgctat caacctggcg
    17461 ggtgtaatag ccttcagtgg taaaaaagta ataatcctcg accttgactt gcgtaaacca
    17521 aaaattcacc ttggtttcaa tgcagaaaat accagaggaa taagtacaat actcatagga
    17581 aaagataagg tttcaaattg catattaagc agttcgctcg aaaatctcga cttcattact
    17641 gccggaccca ttcctcctaa tccttcggag ctaattttat ccaagcgcat ggatgagatg
    17701 gttgactatc ttaaaaccat ttacgatatt atcatcattg atacaccacc agtaggtatt
    17761 gttagtgatg gggtgccaat tattcaaaag gccgattatc cgttatatat acttcgtgca
    17821 aattattcgc gcaaaatgtt tatccataat ataaataaaa tgatggatga aagcaagata
    17881 aaaaacttat ccatcattct taatggagtt gaaatgtcga aacttaaata cggttatgga
    17941 tacggatatg gttacagtta cggatatgga tacggatacg gatacggtta tggttacggt
    18001 tatggatatt acgaagaaga taaaattgac caaagtttat ttgcccggtt taaacgcgca
    18061 ttgccttttg caaaaaagga ggaataagaa atgaaagtaa caaaatttga tattgaaggg
    18121 ccgctattat tggagccaaa agtattttac gatgacaggg gtttgttttt tgaatcgtat
    18181 aatgaacttg aattggcaaa ggccggcatt accgataaat ttgtgcagga caatgaatcg
    18241 gtgtcgaaaa aaaatgtttt acgtggcatg cattttcaag caccgccata cgcacagggc
    18301 aaactggtgc atgttgtgtt tggttcagtt attgatattg tgattgatat acgcgaagaa
    18361 tcgcctacct ttacaaagta tataatgtta actttaagtg ccgataaaaa acagttgttt
    18421 tggatacctc cgggttttgc acatggattt cttgcattgg aggataacac aatttttcga
    18481 tataaatgca cagcaccata taataatgcc agtgagcggg gcataattta taatgatagt
    18541 gatataggaa ttccctggcc gggtgataat tttattgtga gcgataaaga tttggttctt
    18601 cctgcattga aactttcaga aaaaatattt tagctatgtt agaaattaat tcaactatta
    18661 ttaaacataa ctctttgttg atatacggaa gctatttttt gttttcgctt actttttcgt
    18721 ttttgttgaa taagttattt ctaaaatttt cttccacgct tggtaccaaa aataatggtg
    18781 atggttccgt tgtaagatgg agtagtactg caaaacctgt tttgggaggt ctttcatttt
    18841 acattttgtt tttgctttct gtttctatct attttgcttt ttttaatatt gggtcttctg
    18901 ttagttacag caataccttt attggattac ttttatcgat ggctgtaggc tttttggtag
    18961 gacttgctga tgatgcttat aacacaaaac catttttgaa attttcagca caattcgctt
    19021 gtgctttcat tttgattgca accggagttt acattaaagt ttttgacaac ttttacctca
    19081 atgccattct cacaatattt tgggtagtgg gcataatgaa cagtgtcaat atgcttgata
    19141 atatggatgg cattacaagc acagttagtt ctttcattat cctctctgtt atcattttaa
    19201 ttttattgaa tagggattta aacaacttgc accttattat cagcattgga gttttagccg
    19261 ctttaattgg ttttttgttc ttcaactgga acccttcaaa aatgtacatg ggcgataccg
    19321 gtagtcagtt tctgggtgtg ttcctttcgg catttggtat actttatttt tggaacgatt
    19381 actatgttga tctgtcaggg aactactctt tgttaaaaaa gtttataaca gtaggtattg
    19441 cctttttgct tcctttggta gataccacgg tggttgtaat aaacagactc aggcaaggtc
    19501 gttcgccatt tgtaggaggg cgcgaccaca ctacacacag ccttgcattt ttaggactta
    19561 cagatagaca agtagcaata gcatttggtt tgttgagctt aaattctatg tttctggttg
    19621 tgattattaa atattttatt tcgcaatgga gtggagttta tgaactcata tttgcctctt
    19681 acttttttgt cattttagga ttctttttct ttgccactaa caagaaaatt gctgccgaaa
    19741 aaaaaattga acaatatagt tcaactatca ttgagaaaaa cctcgtaagc tcacctcatt
    19801 gatacatatg tttatacctc gcaagattat cgcctttctt ttagccatat ttttcatttt
    19861 gacattagta atctgttgct acaattttag cggtgaaaat aatcctgcaa tggaatttag
    19921 aaagcagaat aatacttttg cattaagctt gcctaatcat ttgcagtttg ccggagaaga
    19981 tctggatttt agcgatgctg aaattgccga acgcttcgat cgagaaatgc atattaatgt
    20041 ttattggcaa tcgcaaacca ttattacact aaaaaaatgt gctcgctggt ttcctgcaat
    20101 cgaaccaatc ttgaaagcac agggtgtgcc cgatgatttt aaatatcttg ctgttgccga
    20161 gagtaatttt tctaatgtag tatcgccaag tggtgctgct ggtttttggc aatttatgcc
    20221 cgatgccgca cgtcaataca aacttattgt gaacgaagaa attgatgagc gttatcatgt
    20281 tgagaaagca acagtggcgg cctgcaatta tttaaaagaa tccaaattga aattcggctc
    20341 ctggttattg gctgccgcct cttataatat gggtgtcgaa ggcgtacaaa ctgctttgca
    20401 aacgcaacaa gtaaaacgct atcaggattt attattgaac gaagaaactt cacgctatgt
    20461 ttttcgtatt gccgcactaa aaagtgttat tgttaattcc gaagcttttg gatttcattt
    20521 aaagaaaagt gattattata ctccttatga atatgatatt ctcactaccg atagcaacat
    20581 caccaatctt gctgaattcg ctttgtcgaa aaaaataaac tataaaacgc tcaaattact
    20641 aaatccatgg ttgcgaaaaa acactttgac tcttatcggc aacatacccg ttccgctaaa
    20701 aattttgaag aaggattatc gtagctttaa aatgtatgat gataaaattc tcaacaagga
    20761 ttcgatttaa aatgaaaggc aacattcatt ctacattgcc aataaacatt atttcataaa
    20821 aatagacatc agcactttta ttccaaaaat aataagtgct gcgcccgata taacattcat
    20881 ccaccgaatt actttcacat tgagtatatg ttgaatagat gctgctaaat atgattttgc
    20941 aatatcggtt gtaagtacca ttgccaaagc cgaaacatag aatgcaatgt tttgcgattc
    21001 atcgaaattt tttgatgcaa ttgcgcccgt tactccaatc caaaaaataa gtgcaaatgg
    21061 attgagggta tttgtaataa aaccactgaa aagattgcgc aaaatattac gcgtgggtac
    21121 aacatttttt aagtctgcct gggtttttag ttcagctttt ttaagtatgc tttgtaatcc
    21181 ccacactaat aataatccac ccccaaaaat tgctgctcct ttttgtacta ttggatattg
    21241 acttataaat ccgttgctga tatgagctaa gaaaatacag aatgcatcgc tcaacattac
    21301 gcctgttgct atggacactg cgggtttaaa acctttattt atactggtaa aaagtatgag
    21361 aaaaaaaacc gggccaatta aaaaggcaag gccaaatccc aaaataatac cttgccaaat
    21421 cgcctctgcc atacactaaa aaaatcttat tgcgtcacgt attgtttgtt gtaatttatc
    21481 cagtcttcga gcattttctt tttcatcaca cggggaaatt tattttgtgc acccactttg
    21541 cctttggttt ccatccaact ataaaaaact tccacaggta ctacatccac ataaatttcg
    21601 cgcaaagccg atgtgcgttc tactctgtaa tcatcgttga gtatttttag tttttcatct
    21661 aatcgtaact tcaatacttc ggcatcggct ttgtcgtgtg tgcctatcca ccagtgatgt
    21721 gcgaacatac ctccatccgg aatgcccgca actgtatatt ccaaaataga aatgtttaat
    21781 tcatcactta ccatctcaat ggccttattc atattgtcga cagataaatg ttcgccacac
    21841 aaactcaagt aatgttttgt gcggcctata attactattt cgcaattttt tttgtttaca
    21901 aattttatca catcgccaat taaataacgc caggctcccg cgttactgct caataataat
    21961 gcatactctt cgccttcttt tacttcattt atcaataagg tttgaggctt gccaattatt
    22021 tctccatcgc tggtaaaatt cttttcgttg tagggtataa attcaaaaaa taatccattg
    22081 tttaggttga ggcgcatttc ggcatcgggt gcttcctgca tagcaataaa tccttccgat
    22141 gccaggtatg tttcgatgta cttaatagga tgtgcaagca aatgctcgaa acttttttta
    22201 tatggttcga aacttacacc accatgcaca tacaacgaaa gattgggcca catatcgtga
    22261 atggttttta gcttatatcg agcaattatt ttttcgagca ataattgtat ccacgcaggc
    22321 actcctacta taattcctat atcccagtta ggtgccattt cagtaatctc ttcgagcttt
    22381 gtggcccaat cggtttcttt ggatatacgt ctgccgggtt tataaaaatg ttgaaaccaa
    22441 aatggaagtt tactcgcagt tataccacta aggtcgcctt caaaataact gccgttgcga
    22501 ttgaggtggg tgctgccacc aagcattaat attccttttt gaaaagtatc ttccgataca
    22561 ttgtaattgc gcagtgtaaa aatttgtcgt atgctttgtt tttgaatgct ttttaccata
    22621 tcgcgcgtta ccggtatatg cttgctcgat gagtcgctgg tgccgctgct taaagcaaaa
    22681 taacgcacct tgccgggcca gcatacatct tcctcgtgca aacttttgtt ccaccacttt
    22741 gcaaatatgg aagtgtaatc gtgtatggga actaactcgc gaaacgaatt tacaaaatga
    22801 cggctacgta aaatattagt gaacacatat gtttgtccga ataaagtgta ttgcgattta
    22861 cgcaaaagtt ttttcaacac tctgcgttgg taatggatag gagtattcaa ttcgtcttgc
    22921 ttcagcaatc cgcgcaattc tattcctttt tttatgatgc tacctaatat tgccacttat
    22981 ttttttttgg gatgcacaaa tatattaaat attccttcaa ttggtgggtt acatgttgtg
    23041 gttttggatt ccattttgaa atgggcattg acacattttg catgtaattt cgcaaataat
    23101 attcactgtg ccaaaaacta aaaaccttct aatgcagcct ccactaaaaa agattttact
    23161 tcctcaatct ctacttcgaa ccccggcata aattctactt gctcttcgaa gctgatatct
    23221 tcaaaatagg atagttgttt aataaatagg gaagggctaa aaacacccgg gaaaagtcta
    23281 atggcttcat tgcaaatttc ggttatcgaa tagaaacctt ttatgataaa atataaatca
    23341 aggtaatctt tccatttccc tctgccacct aatgcgaagg ctttcatggc tgctagagtt
    23401 agtaattcag ggattctaaa atatttttca tgaaatattg ttgcatttat aataaatgga
    23461 aattgaaaaa atgtgagctt aacatcattg attataaaat gaatttggtc aactttttct
    23521 acaatgatat atgatttaaa aggggattgt gaaacagctt tctttatttt tgttttattt
    23581 attggatgta ttgtgaataa atcaaaatca attgacgatc tgtggcctaa gtgcaatgca
    23641 atagctgttc cgccaaccat gtaataattt ttagtatatg gttgtatgaa tggtagcatt
    23701 tcattttgcc ggccattaaa aatttcaaga tgcatatttt aaaaaataaa gggtgaagaa
    23761 gttacgagat attttttcgt aattatttgc cgctctttcc gatttgttga tttcttcaaa
    23821 aaaaactttt gccgcatttt ttatccccac tatttcaaac aattctttta tactttcctt
    23881 atcggcataa ttgataaaaa attctaacac aactggcaag ggcaaatttt ccttttcgct
    23941 gtcttttgta taccaaaaca aatgtgcgtt tttgcgtatc aatgctttta gtgcagggct
    24001 attttccatc tctcaaagtt aacaaattgt ttgtttaaaa tcaatttctg aaacataaat
    24061 ttaagcgcga ataaatttga tgttaatgca ttaaaaaaaa gagtccactc cgaaaagtta
    24121 ttttgccaca aaaacacaaa ggctcaaaat ttcactaagt cgtaagctaa tcattttcaa
    24181 tgcttcgtgg gatttggtgt tttagtggcc tattatttca ttagactttt cggagtagtg
    24241 tcaaaaaatc aacttgcaaa atgtcgtctc tttgcatggc attacagcag aattaatgtg
    24301 aagcgcaaaa ttcttaaatc ttggatttta cactttcgca ctatagaaaa taagccctaa
    24361 ccaacgcaag tagctttaaa taaaaataat tctatatacc gttaaactct tgaatgccga
    24421 cacttcggtc ggcatcccga ccttagtgtc gggactatta aacctttgaa caactgaact
    24481 cttcaactat taaacctttg aactcttgaa ctttccccta cattcccttt ataatctcat
    24541 caccaaactc cgaacacttt acgagtgtgg cgccatccat ttggcggtga aaatcatacg
    24601 taacatgttt gtttgcaatt gcattattca ggcccttaat aattaagtct gcggcttcct
    24661 tccaacccat gtaacgtaac atcatttcgg cactaagtat tactgagccc ggatttactt
    24721 tgtcctgatc ggcatacttt ggggcggtgc catgtgtggc ttcaaaaatg gcgtgacccg
    24781 ttacataatt tatatttgct cccggtgcaa tacctattcc acctacttgt gcggctaatg
    24841 catcgctcaa ataatcacca ttcaaattta atgtagcaat tacatcgaaa tcttcgggac
    24901 gggtaagcac ttgttgcaat gtaatatcag ctatcgaatc ttttataatt attttaccgg
    24961 cagcttttgc agcatcttgt tcggaattgg cagccgcttc gccacttgct tttttggtac
    25021 gttcccattg cgaccatgtg taaactttat caccgaattc tttttcggca acttcgtatc
    25081 cccagttcaa aaacccgcct tcggtatatt tcataatatt gcctttgtga actatagtga
    25141 cactcttgcg tttttcatca atggcaaatt gaattgccgc acgcaccaat cgagctgtgc
    25201 catgttgcga aacagccttt ataccaatac ccacagtaac tttattgcta ctcgatttat
    25261 cgcccgatac tgccatccat tcatcgatac tttgttgcgt accaaatcga atttttttaa
    25321 aatcctttgg gtatgcctct tgaaaccatg ttaaaaattt cgtattattt tcactgccac
    25381 ctgcgtattc tataccggca taaatgtctt cggtgttttc acgaaatata gtcatatcca
    25441 ctttttcggg atgctttaca ggcgaaggca caccggtaaa atattgtacc gggcgaagac
    25501 aaacatataa atctaaaatt tggcgcaaag ctacattcaa cgaagtaatt ccaccaccaa
    25561 ctggtgtagt aagcggccct tttattgcta ccaaatattc gcgaattgta tctaatgttg
    25621 tatcgggcaa ccaattgttt gtttggtcaa atgctttttg tccggcaagc acttcgagcc
    25681 attctatttt gcgtgtgccg ttgtatgcct tatttactgc ggcatctatt acacgcacgc
    25741 tggcacgcca tatatcgcga cccgttccat caccttctat aaatggtatt accggattgt
    25801 ttggtacagt caattttcca tttgctattg ttattttttc tccactcatt ttttagttat
    25861 ttagttattg tgttattcgt ttttagtttt tagtttttag tttatagttt ttagtcccga
    25921 cactacggtc gggatgccga ccgtagtgtc ggcattcttc gtttatagtt tttagttctt
    25981 tgattttagt tgatcgtttt tgttcttcct ttttcgttgt ccgtttttaa tttttaatcc
    26041 tcaatcctta atttataatt cccaatcctt aatttttaat tcttaatcct aaaccctcag
    26101 ttcttaatcc gatttcctga tttttcgcgt gcgaaagtag cattatagtt ttaattgaaa
    26161 tattctgcca aaattttttt tcgaggtatt gttggccatt ttgaatgaag actactgcat
    26221 aacaaccaaa aggcttttcc agtttattga aatttacttt aaagctattt cgtgaaatgg
    26281 aaaagaaagt aaaaactaat aatcgcttgc acgaaatact acacctatca aatttgaagc
    26341 attattgtgt aatgatttat ttgtgcttaa aaggtattat accgaaacaa cttagatttt
    26401 ggaaaatttt tcttttgaag ttgttcggca ttaccagaag taagcagttt tttttcaaaa
    26461 acctgctaac ttttggtata aatgtgtcgg taataaaaca ttttaaagta tgattgaagt
    26521 tgagaataaa atgaaaacta tatatgtaga aacttcaata acccattaac tcagcaaccc
    26581 aataacttaa caactcaata gcccaacaac ctattaacat aacaaaccaa caatcaatca
    26641 acctattttc actatcattg cactacaaaa aaaataacgc actacaatgc ttcaagattc
    26701 gtttggccgt aaacatgatt acttacgcat atcgcttacc gatgtgtgta attttcgatg
    26761 tgtgtattgc atgcctgcgg aggacatgca atttatgcct cctgcgcgat tgatgcaagc
    26821 caacgagatt gttgacatag ctacggagtt tgtaaaatta ggcgttacaa aaattcggct
    26881 tacaggtggc gaaccacttg tgcgcaagga tgcggcagat attattttgc aactttcgaa
    26941 actgcctgtt gaacttacca ttagcacgaa tgcagtgcgt gtggatgaat ttattgatgt
    27001 gtttaagcgt gcaaatatta agtcgataaa tgttagcctc gatacattga acaaagaaaa
    27061 attccacacc attgcccaac gaaattcttt tgataaagta tggagcaata taaatttgtt
    27121 ggtagaaaat aatttccatg taaaaataaa tgctgtaata atgcgcggca taaacgaaac
    27181 agaattgaat gattttgttg ctctcacaca acaacttcct gttcatgttc gctttataga
    27241 gtttatgcct tttacgggca ataaatggaa tgatacaaaa gtgttttcgt atcaacaaat
    27301 gctggatgtg atagcaacaa aatttacata cataaagttg aaagatggaa agcacgacac
    27361 cacaaaaaaa tataaagtgc tgaaccatgc cggaacattt gcagtgataa gtactatgag
    27421 cgctccattt tgtgagggtt gtaatcgcct ccggttaaca gcagatggca aaatgaaaaa
    27481 ctgtttattc agcaaaggcg aagcagatat actttcggct tatcgcaaag gcgaagattt
    27541 tattgccatc ataaaacaat gtgtgcatca aaaagaaaaa gaattaggcg ggcagttcac
    27601 ttccgatttg cacaaggtta atagcgaaaa tattttgaac cgcagtatga taagcatagg
    27661 tggttaaaac acaaagttac agccttctcc aataatcctt ctgcacattg ccgatgtttt
    27721 caacactcat aaccgctatg attagctttc tacatttgaa atcattattt tctgtcattt
    27781 agttttctta ctttagccct attaaaaaat tggaccatgc ttaaaaaaat atttttgttt
    27841 tcaatactca gtattgccgt gcaattttgc aatgcacaag ggcttcaaac ttttcacagt
    27901 tttaccggta caactatact tggcgatact gtttcgtttt cgcaatgggc aggcaaaaaa
    27961 ttattgattg ttaatacagc ctccttttgt ggatacacac cgcaatttac cgatttgcaa
    28021 gctttagatt ctatatatca agctaacaat tttgcagtaa ttggttttcc atgcaacgat
    28081 tttggcgcac aggatcccgg tgaggatagt gtaatcaata atttttgtac cggtttatat
    28141 ggtgtaaaat ttcaaatgat gagtaaggtg ggcattgccc tgcccgatac tgcgccctgg
    28201 tacaaatggc tgcaatggca atatctgaat ggtgttgccg atgcacaggt aacatggaat
    28261 tttaataagt tctgtattga cgaaaacgga cattgggttg cacattatcc ttctaatact
    28321 ttaccattcg atacggccat tgtaaattgg attttatcgc cacctgcaaa tacttctatc
    28381 acagagcaaa caaatatttc gcaagtgcat ttgtttgcta atgctgctgc aaacaaattt
    28441 gagttgataa ttcgcgctga aaaaaacaca gatttattta tccggttgat aaatgcacaa
    28501 ggagccatta tttcgaattt gcataagaat attttatcag gcgaaaacaa aatctcgttc
    28561 gatgctgaac tcacatcggg aatttatttc gtgcaaataa atggtgctaa ctataatcaa
    28621 tgtttgaagg caattaaatt gaattagtcg agcgcctcag ccaagtcgtt tcagccattg
    28681 attagtgcca cacgtcagcc ttggtttgtg tcctcacaac gtcagccata atttgtgttc
    28741 tgcacatcag ccatggtttt tgtcctaacg cgtctgccat ggtttgtgtc ctgcacgtca
    28801 gccttggttt gtgtccccac aaaccatcat ccgatgccgt ttttctcttg ccaatggccg
    28861 ttgttttcac cgactataaa aacgaatgcg tagggtcgaa gtctacacca gctaaaaaaa
    28921 taaattcaca caacttcttt ggttggttag ggaacgccaa ccaaacgaag tagactcagg
    28981 ggaatagcga aataaattta ccatcactaa taaaagtttt cctttacatt tacaaaacca
    29041 atcaaacatg caaaaccaaa aattaaatct acatcaccat gaaaaagcaa ctcactattt
    29101 tatttttact cctcctacaa agttgtttag ctataaccat atacgcacaa ggtggcactt
    29161 ggacttggat aagcggaagt agcaacctga attctttggg agtttatggc acgcagggag
    29221 tgccatctgt aaacaaccac ccaccaggcg tttacgagac atgcgaatgg aaagataagc
    29281 aaggaaattt ttggatttat ggtggtatag atttttattc agatttgtgg aaatttaatc
    29341 ctactacatt agaatggaca tggattaaag gtaatggctt taaaaatcaa gtacctattt
    29401 atggaacaaa aggagttgct aatttttcaa atactcctgg attgattggt tttgctgcga
    29461 ctacatgggt agatacttca ggaaattttt ggctatttgg aggtggatat tcatccaatg
    29521 ctttatggaa atatgagcta agtacaaata tgtggacatg ggtgagtggc agcaccaata
    29581 caaatgccta tggaatttat ggtattaaag gtattcccag tattttcaac tcaccaggta
    29641 taaggcagga aacatgctcc ggttggactg attctttaga ttgtctttgg ttatttggcg
    29701 gaaaaggcaa tggggcatca agttatggtt ctttgaatga tttgtggaaa tttaatataa
    29761 ctaatggtga atggacatgg atggctggtg acttgattgt aaatgtttta tcaatttatg
    29821 gaattaaggg tgtgtctaat tttaacaaca atcctggtgg aagatggtca tatacaaaat
    29881 ggaaaaatat gaatggagat ttttggatat tcggaggcgg ggaatttcaa gacgtaaaaa
    29941 atgatgtttg gaaatttcaa cctgccttaa atgagtgggt gtggatgaat ggtagtactt
    30001 ctgtaaattc taccggcaat tataatattt catgtttgct cagcgctttt gatttgccat
    30061 tttctcgtat cgaaaatcgt tcatcgatta cagataattg tggtaggttt tggacttttg
    30121 ctgggttcag aaataacaat aattctgcat taaatgactt atggattttt gatccgcaac
    30181 aattgaaatg ggttttacta agtggttata atttgacaaa tcagtctggg agctatggaa
    30241 ttttgggaat tccttcactt aacaatcagc cggcaagtag aggatgagct gtagcatggt
    30301 ggggtcagaa taataaattt tatctcttcg gtggatcaca agctatttca tatatgactt
    30361 actgtgacat gtgggttttc gatcccgaca caaattgcat aagcctcaac tgcaatacta
    30421 atatttcaac aattgccaac ttctcctcct ccgattccct cttctgcgaa aagcaatgta
    30481 ttgattttac agacatttcg caaaacaatc caacctcttg gcaatggaca tttaatggcg
    30541 cttcgccttc aacatcctca gaccaaaacc caacaaatat ttgttataat aattatggca
    30601 gttttgatgt aactttggtt gcatgcaatc aattcggttg tgatacaata acaaaaaata
    30661 atttcatcac atcatacatc acaccgttgg atagtatttg gtttactaac gatactttgt
    30721 ttgccttacc tgcatacagt taccaatggt acgaagtagg cacagggctt attgccaatg
    30781 caacacaaaa ttattatgtg ccattaactg tgggtaatta ttattgcata acaaaagata
    30841 cagatggttg cgaatcaaca tcgaatgtga ttttgtacac cagttttttt gaaaatgaca
    30901 accatgaaat atcaaatgcc gtatggccaa atcctgctaa cgaagtatta tttgtaaacc
    30961 cgcaattgct aaacaataaa aatatcattt tcgaaattta cgatatgcaa ggcagaatta
    31021 ttttgcaaca gctgttgagt aacaataaaa gcacaatcaa cattgctcac tttgccgcca
    31081 gcgtatacgg ttaccgtatt atgaccaatg aaaaaataat tgctcaagga agaattgtta
    31141 aggaaattaa gaattaaaaa ttatgaattt gggctgcgcg ctaggaacgg tttgtgtcca
    31201 tggtttgtat ccccacatgc caccaccacg tttcgtgcga cagcctttgt tggagttttc
    31261 tccaacaacc ttatcagcca tgatgcacta attattttgc acacggccat tgtttttacc
    31321 ggcaatcaac tcgacaccac atttgcgcgt cagccaaggt ttgtgtcctc acaaaccttc
    31381 attgcgatgc catttttcgc attgcttatg gccggtgttt tcaccgacca taaaaacgat
    31441 aacgtcacga aataccacgg attaaaatcc atgtttacaa tatgccccgt tcctacggaa
    31501 cttttgctgt gtgtacctgt gtgtgtaagt aattgtgcgt aagcaataga gccgtaggct
    31561 cgactccatt ttgtagggat ggatttcaat ccatcccatt atgcatgcac caaccaagtc
    31621 gcgccagcca tggtttgtgt ccccacaaac catcacgaaa ttccacggat taaaatccat
    31681 ggttacaaca ttctccgttc ctacggaact tatgctgtgt gtacctgtgt gtgtaagtaa
    31741 ttgtgcgtaa gcaatagagc cgtaggctcg acttcatttt gtagggatgg atttcaatcc
    31801 atcccattgt gtatgcgcca gccctctttg gttagatatt gttggcttta gctcaatcaa
    31861 cttgattggt agcagtattc aattttgtga tcaataatta gtaaatctgt aaaaaaatgt
    31921 aagtatttaa tttttactga caattaattt ttggttatta ctgtagtctt tcccgctaca
    31981 ctttattaag taaatgccag gtaaaatgga ggatatatct atattggtat agtcttcaat
    32041 actgttcaat gattttttca aaagagatgc tccacacagt gtgaaaattt caatagaact
    32101 attcgttgtg acttgattag gttctatttg aattgaaatt gtattagttg caggatttgg
    32161 ataaatgagg aatgtttcct cctttttcga ttcttgtaaa tgagccaagg tgggcaagtt
    32221 agcactccac attcctgaac cccaaccccc agcaattact ttattatcca caaaagtaaa
    32281 atcgagtatt tccggatcta ccgggagtcc gaaattttct tcaacccatg tattgccaga
    32341 atctgatgaa aaaaagacag ttgattgtcc cgaattattt gctgaggatg caaatccaaa
    32401 ttgtccgtaa aatcctaatc ccattacatt ttgccctgcc attcctttgt aaatccaagt
    32461 tttgcccgta tcattgcttc tatataatcc gttggtagtg cctgcataaa ttaccccgtc
    32521 tttaaactct aaatcccagc cacctgccat attttgggca acatgcgatg tataggtttg
    32581 accattatca ttcgaataat aaagtgtaaa agcaccccca gaacatcctg caattattgc
    32641 attgccttcg gtggccacag tgaatgtatt tataggagtt gatttagtaa accatgtttg
    32701 gccatcatcg actgtgcttt ttatttgatc gtatgctgct atcacgactg cattgtttgt
    32761 agtagcaatg tcataagtta ctggcatttg gttagcgcca ttgtctatcc ttgtccatgt
    32821 tatgccatca tttgctgtat atcgtatacc acccacaaag caattgttga aatcgccata
    32881 tattgtatca ttttttattg caatactgta cacgccacca ttcaaacctt gataaattgg
    32941 ttgccatgtc agtccagaat ccaacgacat atgcacactt aagtaataag tgcctttgta
    33001 aagttttgtc tttgcattgt tccatgcaaa agtgcttgcg ccttctgctt gttggccata
    33061 ttgagtccag ttaagatttt gcgcatttga atacaggtac ataaagagca gcatgaatgt
    33121 tataagtact tttttcattt tgaatttatt aagagttgga ttaatcacat gcgtccaatt
    33181 ttcaagccag agattttttg caaatttatt taaatcgtat ttctaattgt tttttatttt
    33241 ttctcaattt ataccgaaac aacttaggtt ttggaaaaac aagctatcga agttattcgg
    33301 cattactaac agtacgttat attttcattc gaaaatctaa ctactcttag tataatccaa
    33361 tatcgtattt tgttgagtca taggtttttc ggtttttaag aatgattccg tgcatcagcc
    33421 attttgtgta attgttttca attcacaccc tacactttgt taacgataaa cttcaacaaa
    33481 agcagaattg agcactatgc atttcgaaac atcccccaaa ggttaaaccg atgttattag
    33541 ccctgatgct gccggcatcc tttgcggccc tcaaggcgca aagatacagg caagcagcag
    33601 gaccaagccg ttgatagagc gctacacgct gtgctccaaa tacttcacag gcagaaaata
    33661 tttataaaaa taaattgcta aaatatttag tataataaat tatttagtat gtttggtgct
    33721 taaatattgg gggttatgtt tttaaattgt cattcatatt atagtttgcg ctacggcaca
    33781 ttgccggtgg agcgccttgt agaactgggt aagcagcatg gtgccgaggc gctgtgcctt
    33841 accgatatta ataacgtaac gggggtgttc gattttgtga aggagtgccg cgcacagggt
    33901 ataaagccca tagcgggagt ggaggtgcgc aatggcgata cacatttata tacgttgata
    33961 gcgcaaaaca atcatggctt tgcggagatt aacgaattta tgacgggtta taatttgcgc
    34021 catgccgatt atccgctgcg cccttacgga tttaattatg tacatgtgat atacacgctc
    34081 gatacgtatg agagctggct tggcaccggg cacaagggga cgtatacaag tacagttaac
    34141 aacgagccga tgcgcacacc tctgcccggc accgatggtg gcatggctgc aacaatgcgt
    34201 atagtacgtg cggtggaagc ccctgccgaa agcaccgacc gtggcccaaa gttgcgcgac
    34261 tatgagtgga taggcgtgcg tatatcgcgc ataaaccgcc tggtgcgtgc cgatagcagt
    34321 gtgtacaata agtgtgtggt gatgcaaccc ataacctaca ccgatgagca cgagtacgat
    34381 atacattgcc tgctgcgcgc ggtggaacat aatacactgg taacaaaact ggaagcgcac
    34441 catgtagcac cacgcgatga gcactttact aacgtagacc atatacgtag tgtgtatgca
    34501 cagtatccgc aactgttgca caataccgaa aagctgatgg atacatgctc gttcgacttc
    34561 gactatggca ctataaaaaa ccgcagcacg tttaccggca accgttatga tgataaattg
    34621 ttgctggaaa aattgttgct cgatgggttg gaatatcgct atggaaaaaa taacagagaa
    34681 gccaggcgca gggtgtacag cgagcttgat gtgatagata agctgggctt tacaagttac
    34741 tttctgataa cgtgggatac tatacggtat agcatgagtc gtgggtttta tcatgtgggg
    34801 cggggtagtg gtgccaatag cattgcggct tattgcctgc gcataacgga tgtggatcct
    34861 atagaactgg atttgtattt tgaacggttt ataaatgcgg cacgtacaag cccacccgat
    34921 tttgatattg actacagctg gcgcgaccgc gatgccgtaa tcgattatat atttaagcgc
    34981 tatggcagca agcatactgc cctcatgggt actataagca catttaaggg gcgcagcata
    35041 ttgcgtgagt tgggcaaagt attcggcatg cccaaaggcg aaatagatgt actggtaaat
    35101 aaaccattgg atgccgccaa cgataccatg ctatgtaaac gcattataaa ctatggcaac
    35161 agcattgccg attttcccaa tcagcgcagc atacactcag gcggtgtgct tattagcgac
    35221 ctgcctatat ttcattacac ggcattggac ttgccgccca aggattttgc cgttacacaa
    35281 tttgatatgt atgtggccga aagtataggc ttcgaaaagc ttgatatact tagccaacgc
    35341 ggcatagggc atatacagga cagcgtaaaa ataatagagc aaaaccgcaa agtgcgggtg
    35401 gatgtacatg atgtaaaaac atttaaaacc gatgaaaagg taaatgcaca attgcgcaaa
    35461 ggcgatgcca taggatgttt ttatgtagag agtcctgcca tgcgggggtt gctaaaaaaa
    35521 ttgcgttgtg gcgattacat tacactggtg gcggccagca gcattatacg cccgggtgtg
    35581 gccaagagtg gtatgatgcg cgcttatata caacggtttc acaagcccga gtcggtggtg
    35641 tatttgcacc ccgtgatgga ggagttgctc aaagaaacgt atggcgtaat ggtgtaccag
    35701 gaagatgtaa taaaggtggc gcatcacttt gccggcctcg accttaccga agccgatgtg
    35761 ttgcgaaggg ccatgagtgg taagtttcgc tctcgcaagg agttggataa aatagtagaa
    35821 aagttttttg ccaattgtcg cgaacgtggc tataacgatg aacttagtaa agaagtgtgg
    35881 cggcagatag aaagtttttc gggctactcg ttttcgaaag cgcactcggc aagttatgcg
    35941 gtggagagtt atcagagttt gtttttgaaa acttatttcc ccatggaatt tatggtggcg
    36001 gtgataaata attttggtgg cttttaccgc acgtgggtgt atgtgcacga agcacgcatg
    36061 gctggtgcgc aaatcgaatg cccttgtgta aacacgagta catatcttac aagcataaaa
    36121 gggcgcatta tttatctcgg ctttatacac atcgaaaata tggagcagca gtatgccgag
    36181 cgcattgcag tggagcgcaa cagaggtggt gcatttactt cgctcgaaga ttttgtacta
    36241 cgtatacaca tatcgttaga ccaggtgatg ttgctcattc gccttggtgc ttttaggttt
    36301 accggtattg gcaaaaaaga attattgtgg caggcgcacc tgttgctaaa caaaggaaaa
    36361 gtaacaatgg aaaccatgcc gatgtttatt cccgaaacaa aaacatatcc tctgcccaca
    36421 ctcgaacatc accgccttga cgatgcctat gaagaaacag agttgctcgg ctttccggtt
    36481 acatgcacgc gcttcgattt gttgaagact aactttcgtg gcgatataag tgccgcagcc
    36541 atgccccaac acataggtaa aaaagtgcgg ctggtgggcg accttgttgc cataaaacat
    36601 gtaactaccg taaagcgcga gtacatgaac tttgccacga tgatagatgc cgaaggaaat
    36661 tttttcgata ccgtacactt tcccgatcag ttaaaaaacc atcccttcaa tgggtatggc
    36721 atttatatgt tactgggcaa agtggtagag gagtttggct tctgtatgat tgaagtagaa
    36781 aaaatggcca agatggaatt gcaggcggat ccaagatatg cggggtaggg gggattgtca
    36841 ttttttgaat acttgtcatt atttaattct tgtcattggt cattgttgtt gtggcaattg
    36901 tcatgctgag gaacgaagca tctctgccaa caatgatgga ttgtcattgg tcattatcgt
    36961 cattgcgaat actcacagtg gcaattcaca atgacggcag gaaaaaaaga aaaccgaccc
    37021 agagtcctca ttcttttttg tttttttatt tgcatgcgag actctgaatc taataaaagc
    37081 aaatacaata agcatcacaa agttgtgttc tatgatttgt tttcaaaagc gaaaatagga
    37141 ttcgacaata atttggagat gtttgaaatg aattattttt tcatttgtag atttccctat
    37201 attcgcttca cctaaaccaa attaatatga agaatttttt aaaaacattt ttgatttcaa
    37261 tttctttttt cggtgttgat tttgcacatg cccaatcaaa tactaataaa tttactccac
    37321 cacgttttga ttttgaaggc gggattgaaa cttatatgtt acaaaaattt gatgatgcac
    37381 aaatcatgat tgccaacatt aataaggatg cgtattcagc atctaaattc acaataacaa
    37441 gcgatggtac tattcaattt gtggcagata gtgtttgggg aaacaataat gatttgaata
    37501 ataaaatttt gcaataccta aaagagggtg agcaatattt taaaatgcca atgataaatg
    37561 gaaacaaagt tgcactaaca acatatgtat actttgatat aaattatgac tcactagcaa
    37621 ataaatattt accctcattt aaacttgatt acgacttaat atcgctgagc agcgcatata
    37681 tgaatgaagg cacgaagatg ataagcgaaa tgaaatatga tgaagctata actttatttt
    37741 atgaagtata ctatacaaat ataaacgata tgactgccta ttataatatt gggttgtgca
    37801 agctaaagtt aggcgacacc ttaagtgcat gtgcttattg gaaagaaact cgaaaatata
    37861 acagtgccat tgcaaacccg gaaatttatg aatattgttc ggcacctaac tcaataatag
    37921 aagaacgaat ggctgagatt aaaaaagacg aaaattccag atatcccgag caagtattta
    37981 caatagtgga gcacatgcct gattttattg ggggagaaaa agaactaaaa aaatttgtgc
    38041 aaacacgatt tcattatcct aatgtagaaa aagaaaaaaa cattggagga actgtatacg
    38101 ttacctttat tgtaatgccc gatggaagca tgaatgattt aagaatagtg cgtggcatag
    38161 ccggagcgcc atttttagac caagaggtaa ttcgtttagt aagacagatg cccaagtgga
    38221 atcctggatc tcaaagtggt aaaattgttc ctgtacaatt taatttacca ataaaatgct
    38281 cacctattac aaattaagtg ctcaaagatt tagcctcatt attattgctt gtttttattg
    38341 aaacattgaa ttttatttaa cccttgtacg aaaaatattt ccctacattc gcttcaccta
    38401 aaccaaatat ttatgctaat gaaattgaaa ttgaatttgt tgtttgtttt gatgttgttg
    38461 tttatgcaag ttgatttaaa cgcgcaacaa gcagatagca taaaatatca acctcctgtt
    38521 tttgattatg agggcggcat taaagcatac atcaatagta aactcgaata ctcgaaggag
    38581 ctaaataaaa aatatcccga aggaatggat tgggtgatga attttatgat aacccgcaat
    38641 ggcatgatag agtttgatat gcgcagtatg cccgacacaa ataatatgtt tgaagtggaa
    38701 gtatataata ttttgcaaca aggtcgctct tattggaaag cggccacgct cgataatgct
    38761 gttattgtta ccgattgcga tatgaacatg aatgtacact ataacaaaaa agaaaaaaca
    38821 gtaagtgccg atattaattt gcgattcgat aaaaataaac ttgccgagaa ttatatggat
    38881 gtggcgctaa caaaactgaa agcaagcgag tataaaaatg ctttgcctta tattcaagca
    38941 gcaagtgatt taagcccggg caatatggaa agaaaattac aattgggaat ttgtaaaatg
    39001 aatataggag atacagtaga agcatgcgaa atttggagaa agataaaagc cagtaacata
    39061 aaaattgccg atgatcagat tgcaaaatat tgtggtgcta ttgcaaaaaa gaatgatgat
    39121 gaaaacaaac cgaataccga aataaaaacg gtggacgaag taatgcccga atttcccggt
    39181 ggacaagcag agttggttaa ctttataatt aaaacatttc attatcccga aaaagaacgt
    39241 gaagcaaata ttgaaggaac ggtatatgta agttttgtag taaagaaaga tggtaatata
    39301 acccacataa gaattaaacg tggcataaag gacggcttgg gactcgaacg cgaaagtata
    39361 cgcatggtga aatgtatgcc ccgatggaaa cccggcaccc aaaatggaaa gcctgtagat
    39421 gtgcaattta ctttaccggt gaagtgtaag ttgagaggtt aataaaaatt tgatatttta
    39481 aagaatggag caatcaaaga attgtttata atcttgattg ttgtaaaaga ttgaaaaata
    39541 atattatgat tacgcattct ttgcatgacg ttaaaagcta atcaaacaat ccaaaaattg
    39601 aaatcttcca ttgatgatta attttttcaa ttacaatact atttgcgcgc tttaaaatac
    39661 agcaataatc ttcataaatc caacctttag tttttttgtc aagtgtagtt acaaaattaa
    39721 aacaacattg aaatttagcc aatgaatctt gcgaagcttg atatgatttg tacctatcta
    39781 gttcaacaag atcataagta agataagcaa taatttttga tgtggaatcg ggcttttcat
    39841 aagccggcac atgaggttta aaacaaataa aagaaccaag gagagtatcg atattgccaa
    39901 tttcatattt gggatgaaag tccccaaaaa aaggccccaa atgtttcata gcattcatgt
    39961 aaggtattgt aaatatttcc cgggtcgagt catagggact gccatatgtt ccaccaattt
    40021 taattgattg taaaattact tcccaaacca tggaatattt tcctgagtcc agcctccaaa
    40081 gaattttaaa attttcaagg ccgacattag taccaacaga agaaattcct ccagaaatac
    40141 caatattgga tgtacttaaa acctcagggt tcagcatttt aaaaagagca gacgtatctt
    40201 cgcgaattgc aattattttc aatgaatcaa taaattcgct tagcccgcta taattttgaa
    40261 tatcattaga tggatatatt ctatacactc tctcgtcctg agcatatccg atttgcaaaa
    40321 taagcgttga aattataatt aaaaagtgtt tcattgtgat ttattctgtc agccaatata
    40381 tggattattt atttttaaaa aaaaatctat tgaagtacaa aacaataaac tcttgcataa
    40441 tgaataacag gaatagcatt cataactctt tttttaatta tttattcttt gcaattattt
    40501 tgtgcaatgt atgcaaccta tcacatgcgc aatctattga tagtgcaatg ttgtacactc
    40561 cacctcagtt tgaatatgaa ggcggattgc tttcgtttgt acaagcacat ttggaattga
    40621 atgacaagat gttttctcaa aatggagata ctgtaaaatg tacaatggta ttctctgtaa
    40681 gcgactcggg tgttttgcaa tttgataaat cttatgagca attcgatata aaaacatttt
    40741 gcaatgcggt gtatactatg ctaaagcaaa gtactcactt atggaagccg gctttgcttg
    40801 gtacgaataa aatcaattca atcatgggaa taacattcgc agtacagcat atcgataaag
    40861 aacaaaatat aatttataat cctgaaatat tagatacgga cacaacttgg cttgcaaatg
    40921 ttttttataa tgaaggaacg cgcctattga atgataaaaa gcatttcgag gctattcctt
    40981 ttttggaaaa ggcaactgaa ataaattcgt ctgatgatta tgcaatatat aatttaggag
    41041 tttgcaaatt agcgttgggc gatacaatat ctgcttgtga attatggtgg caaactattt
    41101 cgctgaaaag caagattgcc aataaaatta tttttaaata ttgtccgaat aaattttatt
    41161 tcgcaaaaaa cgaagatgga agtatcgatt caactattca tttttatacc attatagagc
    41221 agatgcctga atttcccgga ggggcgaatg ggctgataaa ttatatccaa gagaattttc
    41281 gttatccaac agaaagtcgc ctaaataacg ttatgggaac ggtttatctt acttttataa
    41341 tacttgctga tggaacgatc accagagtta aaattaaaag aggaattcca ggaggagaag
    41401 atttggaaca agaagcaata agattagtta aaagcatgcc caattggata ccgggaaagc
    41461 aaaacgggaa attcgtaaat gttcaattcc aattacctat taaatgtcaa ttaactgatt
    41521 atcataaata gtaatttatt gctgatttaa ttattcaatg ttctatctct aaattaattt
    41581 caatcatatt tattttataa ttaatttttg attgaatact ttttccccat cagatatttg
    41641 cactaagtat attcctggag gaaagttgca gcggaaattt tctgatttgt ttttagttaa
    41701 ctcttgctga aaaacattgc ggcctgttaa gtcaaaaata ttgatatttt taattcccga
    41761 ttttaacgtt tcattgaaag tgatattgaa attatctgaa gcaggattag gcgaaattaa
    41821 gaatgaattc gactggaggc cgtaatcatt aattccagcc atatcaccgt taaatcttac
    41881 aacagcatat tgccaatatg tttggtagcg tgcttgtcct acaaccaaaa tctgaccgtc
    41941 attctgtacg gcaaccccat ttgcataatc gtctgcactt gtaagatctg taaaaacaat
    42001 tccccctgtt ccaaatgcat tgtcaagtat accggtacta tcgtaccgca cggttgtaaa
    42061 catttcgtta gccgaagcaa aggggcttct tccaacggca attatttttc cgtcctgttg
    42121 aatgtctact gcattgcaat accaattatg acctggcgca ccggtaattg tttttccgtt
    42181 agtgccaaaa ttattatcaa gtgtgccgtc agtattgtac ctggcaattg cgaaagcgta
    42241 attaccatta gcagaactgg taccggcaac aacaattttt ccatcacttt gcagtttcat
    42301 gccgtaacct tgatcgtcag aattattgaa atcagtagct acttttccgt ttactccaaa
    42361 agtactgtct aaactaccat tggaattata acgtacaacg gcaaaatcaa aagttgagtt
    42421 tccagcgcta cctgctacaa ctattttacc atcaggttgc tggctaaccg aattagcata
    42481 gtcataagag aatcccaaag cggtggttac aattccatca ttatcaaatg tactgtctaa
    42541 agtgccgttt gtattgtaac gcaacaccat catgtcggta ccaccagttg aagtcatgcc
    42601 agctgccagt atttttccgt cagactgtat tgcaactgct ttgcttgaac aatcgccacc
    42661 caccttggta actacttttc cacttgtgcc aaaagtatta tctaaattcc cattagtaag
    42721 atgacgggca acaacactgg cttgtgtgct attattaaat ccggcaacta ctatattgct
    42781 gtcgctttgt atagcaactg cccaacccga cccggcaaca ggacttaaca cctgtccccc
    42841 tgttccaaag ctattgtcat aactaccgtc agtgttgagt cgtaacatta aaaagttgga
    42901 ataattacct agaacaacta ttttattatc cggctgaatt gccataccta aaggctgccc
    42961 atatggagca ccatttgaaa gaaagatacc tccgcttcca aaagttgggt cgagtgtgcc
    43021 agattgagcc agagaaaatt tgacaataat aattaacaaa aataatgttg aagtgatttt
    43081 tttcatagtg atgttttgtt tgaataatct tcaaatttat taaatttatt taattcgcag
    43141 ttgaaaaaga attgctgttg gatatttaca aacgataatg gtgttgatat ttttttaaaa
    43201 gtgattactt gcaattgctt tcattatgca cctcaaaata ttataagtta attataagtc
    43261 gcagcgtttt gtaattatga catttctaat tgatgtttgc ttcgaaagaa ccattaaatt
    43321 ttctccacaa aatacatttc catttcacct ttattttttg ctgaaatttt tccgcgtggg
    43381 gtgcatgcaa aattgttgtg caccaaatta tatgtagtgg tcgaaatatt tattttccct
    43441 tcttcgctgc tactttccat gcgggctgct atattcactg tatcgccata cacatcgtac
    43501 gaaaacttct tctctcccac aacgcctgct acaagcgggc ctgtgtgcaa tccaatgcga
    43561 attttaaaaa atggttttcc cagtttttcg caatcgcgtt gatgcgcaat cattacatct
    43621 ataatttcta tggcagcatt tacgattcta atatgcgatt ggttgtcgtt atctaatccg
    43681 gccacggcca tatatgcatc gccaatagtt tttatttttt cgatagtata ctttgtcatt
    43741 atagcatcaa actttcggta gcaataatca agttcattta tcaattgagt tggggtgagg
    43801 gattcggcaa ccttggtgaa atcaacaaaa tctgtaaaca tcacggtggc ttgcggaata
    43861 ttttgtgcct gtgctttgcc ggtctgcttg agctcgttag caacattttt cggtaaaata
    43921 ttcaacagta agttttccga tttttcctgc tccaccaata attgcttatt gaatttattt
    43981 gcactcgcat agcgataata cattgctgcg gccagtaaaa gtccaagtgt ggcaaccaca
    44041 aaaaataaat tccgttgcga tttctgcaat gcctctttgg tatttgcatt ttccaatgca
    44101 atggtttgtt gcgtaatttt agctgtttgt ccttttatta aaagcgaatc tattcgcgaa
    44161 gcatacgcaa gcgaatcggc cagcattttt tgtacattca atgtgagttg catacgcgct
    44221 tgatcagcac tcatacgatt gatgttctct ttttgttttt gcaattcatt gtcggcatcg
    44281 ctcaattcgg ttgtatataa ttgcttctgt tgctcaattt ctttttcaag ttgagcattt
    44341 tcattttcta atcgaatgcg ttcatttcgt ttaaaagttg ccaacgaatc caatttgttt
    44401 ttcttttgtt cgagcaattt catggcaggc cgtctcggtt tttgcctgct gttattgttt
    44461 tcgctttgag aaaaaacatg caggctcaca agcatcgtta agcctaaaac aaaaatcatt
    44521 tttttatcaa gcaacatatt tcaaaattat atggaaggtt attttttctg caattttact
    44581 tttcattttt gacataaagc tttttcttaa accaacaact tcaaaccaac aacttcaaac
    44641 ttcaaactaa aaacttcaaa ctaatctcac tccccatact tcaataaaaa ttccggattg
    44701 atggctttaa aagtaattct tccgttgcta aatgtattcg ataattctgc atctacttta
    44761 tcgtcataag gtcgtattac aattccttcg gcccacgctt gtgcatccat tgtgcttttt
    44821 atggtggcga gttttacaat agcttcaata tcattttcga gtatgtagtc tctgtttatt
    44881 acaggcacca taggtagttc aagtttttgc aaaatatttt ccagctcctc gaaattgtaa
    44941 tatcgaaaat tgtcgatgtc gaatgccgtg aagaaaaaaa ctttgtgacc acgtatttta
    45001 catttattat tctgaatgcc ttcgccaacc aactcgcctt gcaatgcaat attgatattg
    45061 agcaatttca ttttattttc taaatcattt tcgcgtgcaa ctttccaaaa actatttgca
    45121 ttgtcttctt ccaattccat attgcgacta cacacgccaa aaatattatc ttttacaaaa
    45181 tacgttgccg agctgccatc aagtttttcg gtgatataac attttgtgcc tttgtaacga
    45241 tccaaaacat tttgtaaaat ctgcactcgg gtttcgtctg tcttcggaat aaaagatgga
    45301 aactttcctt tcataattcc tgcaaggcat gcaggtatag gaggctcgta ctttgtaatt
    45361 cccaaaactt cggtcacatc gacatcttct tccactttta ccgaggcatc aagaatgcta
    45421 aggggaaaac aaatgccctg cgaaacttgc ccacgcaatc ttatggtgcg tatacgcata
    45481 ccgcgcactt tcaaaaattc gaattctggt ttgttgggca tcaacgaatc tatttcgcag
    45541 tacacacaca aatcaccaat ttgaaattca ttttttttta ctaccaaatg ccagccaagc
    45601 acagttgctt tttctattgc atcagcacct tcaattggtt cgaggttttt tattttttga
    45661 atgctcgcta attttctcat ttccgatttc tctatttgtg gttttgcttt ttgtgttttt
    45721 tattatgtca gcaatttgca gaaaaaaatt atcccgtaaa aataattgga ttcaatcaat
    45781 ttttgtaata gttgaaatat tacttttgga aaataattct caatcaaaat taaatgaagt
    45841 tatttattaa aaatatagaa agttcgataa acgaagccgt gctcgaaagt ttgtttgcat
    45901 tgtatggtga aatagaaagt accaaaatta tttacgatcg cataacatgg gagtcgaagg
    45961 ggtacggctt tatagaattt acaaaaaagg ccgatggaca aaaagcaata gaagccatga
    46021 atggaaaaga aattcgcggc aaagccttat ctgttgtcga agccgatgaa aagcgcaaat
    46081 aatatctttt gttttcaaca ttattgaact gatgcgcact cttgtttttt ttcgaattgc
    46141 tgtaaaccct ttatttaaag gcatttttgg cttaagttcg ttttgatttt gtagcaatcg
    46201 ggccgtcaca agtttattga agcaaaatat taaaaaataa tttttggtaa ttatttatta
    46261 attctatttt tgcagccccg aaaaaagagg aatagagtaa ttaatacgag gtatattgtt
    46321 caaaagcctt tattagtaag ccttacaaga attttgggag cgttctttaa aattcaatgt
    46381 ttaaatatat tcacagcaaa ttagtttgtt gttttaaaat attgattccg tagctcagtt
    46441 ggtagagcaa tacactttta atgtatgggt cctgggttcg agtcccagcg ggatcacaat
    46501 aaggttgtca gcaatggcaa ccttttttgt tataaaaaaa aatctgcgtt attcaatctg
    46561 cgaatatcag cgctatctgc gggagagtat ttttcccgct gattaacgca gataaattca
    46621 gtcgcagata agcgcagata aatttcgttt gccaaatgca aaggtcagtt ttaaaaaaaa
    46681 tctgcggtat taaatctgcg aatatcagcg ctatctgcgg gagagtattt ttcccgctga
    46741 ttaacgcaga taaattcagt cgcagataag cgcagataaa tttcgtttgc caaatgcaaa
    46801 ggtcagtttt aaaaaaaatc tgcggtatta aatctgcgaa tatcagcgct atctgcggga
    46861 gagtattttt cccgctgatt aacgcagata aattcagtcg cagataagcg cagataaatt
    46921 tcgtttgcca aatgcaaagg ccagttttaa aaaaatctgc ggtattcaat ccccgaaaat
    46981 cagcgctacc tgattgaaat attttctgct tagtattgaa atcatctcca ataaaaagtg
    47041 catctacaaa ctatcctctt ataacaggat gtcttacaaa ctcttttgta cggtcgaaca
    47101 gaaaacgata tgtaactaac acacgtgaac gcacggggca aaattctttt gcaatattca
    47161 acatgcgtgg attgaaatcg cccacccatt gcatttcgaa ttcatcgtat gattttttac
    47221 gttttactat tttttccatt tctacaaaaa tgtatgcatc caatcccttg ccttgatgtg
    47281 ccggaataat tccgaacaca gttccaatca tttttgtgca tacatgcatc ttcagcatta
    47341 caaaaaattt tatcttttgc cacaatccaa atttaccatt cagtttgcga aacagttggt
    47401 ttatatcggg cagacaaatg gcaaaggcaa ccgggtcgtt tttgtaataa gcaaaggcca
    47461 tgatgtcggg atcgataata ggtttaagtt ttttaaaaaa tccctgtact tgtttttgct
    47521 ccaaatcgcg gccactgcca tgttgcaccc aagcttgatt ataaatagaa cgaaaatctt
    47581 cggcatactt ttcaatttca ttcatcttta aagtacgaaa agaatactcc gggatttttg
    47641 aaatctcagc atgccgctga taaaattttt gtggcaaatc ttctagttgt tcaattgcat
    47701 aacaatgttg ttgaaaataa tctttaaacc catacgtttc gaaaagcgtt tgatagtatg
    47761 gtaaattgta gttcatttta tattgcggag gattaaaccc atcaattatt aaaccccacc
    47821 acgattcgcg ctcaccaaaa tttatgggcc catccattgc ttccatgcct tgttccttga
    47881 gccattctgc acaagtgtcg aacaaaatat tggccgcctt ttggtcgttg atacattcaa
    47941 aaaaacccat gcctcccgta ggttgatcat ttttctttgc cagtttatga ttcacaaatg
    48001 ccgcaacacg gcctatcgtt tttccgttat ctgcaagcag ccatcgagca caaatgccat
    48061 gacgaaaaaa tttgtttttt tctttgtcaa atacttcatc gatatctttg tcgagtggtt
    48121 gcgcccagtt tttatcattc ttgtaaagct gcaatggcaa ttgtaaaaat tgctgtgcct
    48181 gctcttttgt agtaacttct attatttgca taagtaaaat gtgtggtgag caaaattaat
    48241 ttttaggttt gtaaaaaatg taattgtgaa aaataaagtt gtagtaataa ccggagcctc
    48301 gagtggaata ggggaggccc tggcaaaaaa atttgacgcc atgggcagta atatagtatt
    48361 ggctgcgcgt gacgaagcta aattgatggc tgttaaaaat agtttgcaaa ataaaagtat
    48421 tgcggtggta accgatgtgt cgaacgagaa cgattgcaaa ctgctcatgg aaaaggcaat
    48481 tgccgagttt ggaagcattg atgtattaat ttgtaatgca ggtatgtcga tgcgcgctat
    48541 gtttaatgat gtgggcctgg aggtgttgca taaattgatg aacgtaaatt tttggggtgc
    48601 cgtaaattgc tgtaagcatg cattgccatt tttgttgaaa agcaaaggca gcattattgg
    48661 ggtgtcttct attgctggta aagttggatt gccggcccgt acaggttaca gcgcaagtaa
    48721 gttcgccctc gaaggattcc tcaatacatt gcgtatcgaa aatttgaaaa acaatttaca
    48781 tgtattggta gcgtgccccg gatatacagc ttctaatatt cgcaatgcag cattgaacga
    48841 aaaaggtcag cagcaaatgg atacaccact cgatgaaaat aaattgatga cggccgatga
    48901 agtagcaaca gaaatatata tggccacaat ggaacgtaag cgcgatttgg ttttaactac
    48961 aaatggtaat tttacggttt ggctcaataa attttttcct gcgtggatgg ataaaattat
    49021 ttaccgtttt gtagcgaaag aaaaagattc tccttttaaa taaaaggcac aatagttttt
    49081 cttgtcaaag catagaatta ttttttgcca cgcctgcccc gattttcggg gaatggagcg
    49141 aatgaatgac gaataaattc tccatgcttg aactctttct aaaaacctta aatgaatctt
    49201 atttgtcttt atggtttgct ttttttaatc gttaataata agtttcatcc acttgttagt
    49261 tccatccact ttcaattgca ctaaataaat gccggatgat aaattggcaa aatctatttc
    49321 atgtttgtta ttgccatatg cgcttacaag ttttttattt aaaatgattt tacctgtcaa
    49381 ttcatacact cccatttcca tttcgccatc ggtatacgat tcatactgca atgttatgcg
    49441 tcccttgccg gggttgggat aaaacgatat tgaattagga tcaattgttt cgttttcatt
    49501 tttgcatgta aacgcaaccg ccaaatgact tgcagcactc gatccgcaac tattatttgc
    49561 cttgcaagtt attatgccat taatatttcc ccagttaacc acaatgtgtg gcgtgccttg
    49621 tccgcttacg attgtgctgc ccggtacaac agtccatgta tacgaagtgg caccggcaac
    49681 aggtgcaacg gtataatgca aaccaggttg gtatttgcat gcatagttaa gcccgcttat
    49741 aataggagat tgtggtctag cgttcaatgt taaagttctt gccgcactca taccacaagt
    49801 attcacagct tttacggtaa tatttcctgt tccagtaaac gaattactga aagaaaccat
    49861 cacagcattg ctgccttgcc cgctcataat gctggcgcct gttggtaccg tccaaaaata
    49921 agatgtggca ctcggaaccg tagcagtact atatggcaca ttgggcttgt tgcacacgcc
    49981 cgaagtttgt cccgatatag ttgaaggcgc acctggaata ctattaaccg tatagtttaa
    50041 attgctggaa gtacttgtgc cacatgcatt tgaagctgtt acactcacta tgcccggcaa
    50101 agcactgcct gtgaggctga aagttataac tgtgcctcct tgcccctgag taataattgc
    50161 tcctgcaccc aaagtccatg tatagcttgt tgcccctgca actggaagta tagaaaaagt
    50221 attgccgata gtattgcgac acaaggcggt agtacctgta attaatcccg gtggtgcggg
    50281 gggtgttccg gttgaagtta ttacggaggt gcatgtattg gtgcagccat ttgcatctgt
    50341 tacagtgaca gtatacgtac cttgagataa acccgaaaca aaagtgcctg tgcttccatt
    50401 gctccatgag taactgctat tgccactgcc tccggatgaa cttaccgatg ctgtaccatt
    50461 attagcgata ttgcaagtaa catttgtgga agtgcatgtt gctgtaagag cattaggtgc
    50521 ggttattata ttaactgatg aagcagtgca tccaataaca tcagttacta taactgtata
    50581 cgtaccttct gctaaacctg tggcagttgc agtagtggcg ccattactcc atgcataagt
    50641 atatggggca tgcccaaggg ttgcagttac agataaaacc ccgttggcaa catttgcaca
    50701 tgttgcattc acaaccaccg gagccaaatt tatcagagta gtatccatgg cataattatt
    50761 ttcgcaatta ccaaacataa ataatcgaac atgcaggcta tcttgattgt taatgcccat
    50821 cgaatttaga agttgcatcg aatctaagtt gtaatgaaaa tgtagcgagt cgtttaaata
    50881 aaaatgcaat ggtaaaaact gacctaccag cacttgatta aaggtgctgt aattacctgt
    50941 ttttacaata ctgctaacca tgccaccatt cacgtttatt attaatgaac cgtttaatga
    51001 tgtcagattg gaaggataaa ttattttgaa ttcggacgaa tcggattcaa tatgtacagt
    51061 atcgttgcgc caataattta gttgagttaa cgtgtccaat cctgtgtaaa aagaaaaatg
    51121 tatactcagc aaagtgccca atgtatcttt accggtgata gaatctttga tataaaaatt
    51181 aataggatcg ttcgaaccgg atttctgaat gatagtgtgt cctttacctt ttttgccaca
    51241 ttcaaaaaat gcttcccagt caatatttcc tttgagtaaa tctttatctt caacaacagt
    51301 aacacctgcc acgctgaatg agtatgcttc ggcacggcac gtgctatcgg atatggcttg
    51361 cgctaaacca tagttttgaa tcactcctga acaaccactt gcaaattcag ccgagcctat
    51421 tgccacacat gaattggcat ggctgtgtga atggcaactt cctgcattag catgtgcctc
    51481 atatattgta tcggggtttt caatttgata actctcccac tttacaggat ttccattgtt
    51541 ggttatacta ctgtcaacac ctaaaacatt taaaccgaaa ttgttgaaat taatattttg
    51601 tggagtaagt ataaaaacag gaatgctgca tcccgattcc ttggcccatg catgattaaa
    51661 ggcaagagta aatatcttcg gagcaataat gttatccgtt gtcaatttaa aatcgtgttt
    51721 cattctatac gttatcacat tatcgcgact tgccgttttt ataagtgaat caacattatc
    51781 gttgcattgt gaacgagaat agtttgcaag cagcgttaca aaaataaata caagaatttt
    51841 tttcataatg atagattatt ttgggaatga taaggccaag gtttcttatt cgcaataaag
    51901 ataaaaacta ttttgtaaag cgagcatttt atttttcgat accaagagtc actggtttgt
    51961 gaatttgaaa acaaaaaaaa tggagtgacc acaaagccac tccatataaa aagcgaaaaa
    52021 tgattttaat tttttgccag cttaatggtt tctttttgac cgtttctgtt tagttgtaaa
    52081 atatacaagc ccttgcttaa cgattttata tcaagtattg cttcgttatt gccttcctga
    52141 gcctgaaagg tttgttccat ggcaagtttt cctgtcatat taaatatttg aatcaatacc
    52201 ggttcggctt tttcatttat gaattgcaca ttggttaatt ctgttgcagg atttggatat
    52261 gctattgcag cacttgatgc ttcaatcaat tcaccattgc ttccttcgcg aaagaaagtg
    52321 ctgcaagtag taattctgcc acaggcgtct gttattgcaa ctgcataaaa ttttggagca
    52381 ctgccggcac atttatatac cgattgcgaa tttgcagtgg tggcatatat tattttggaa
    52441 caaggtacat tccacacata agagtatggc ccacatcctc cggcaacatt agcaattagt
    52501 atctgttgag ttgacgaaat atagttgata gaaaaggtgc atgtaggatt acttttcttg
    52561 ttaggccctt ttggttttaa taaattgctg tacttaacat tgccacagca gtcggttact
    52621 tttgcacgat aaaaataatt cgtatcggca ttatttacgg tagaagtatt attactaatt
    52681 gatacaaaaa gtccgcctga tgccggcctg cgttcccacg aaatcaccaa tggtgcgcat
    52741 cctcccgatg cattcaatgt taattgtcca tcaccattgc agcaagcaag ttttgtttgt
    52801 gttatggttg cagttattgg cacgtaatta atagttgtaa caacaccaca tccatttgta
    52861 acttttaatg atgtaggcac aggaacaggc gggcaaccca aacaagtaag gtaaacaaca
    52921 taggatcggt ttgtaccaat aacatttcca ttaatatcac accactcata agtataaacg
    52981 catccgggcg gtggtaaagg attgggcacc gcattataaa caccattggt aaatgtagat
    53041 gtggtaaaac caatgcaacc agtccataaa gggcaaagca aaacggtaaa acagcacgat
    53101 acagtattgc catcacaatc ggttacagta acacaatacg tacctgccga caagccgctt
    53161 aagtcctcgg tggtagcacc gttgctccat aaatatgtgc ggcattggtt aaaacaattg
    53221 accgttaaat caattgtgcc attaggcaca ttgcctggag tgatgcatgt ttttgctgtt
    53281 atagatggtg cagtaaactc tgccgactgt gtgcatccct tggaatcggt aacggtaacc
    53341 acatacgttg agcatggttt aaaagttgct aaacatggtg cagtgccaat aataggagtg
    53401 gagccttttt cctgccattg ataactataa ggcccaaacc ctcccgaagc acttgcacat
    53461 aaagtggtgt tgcaagtggc cgaaaaatta atcaagatag gtggtggtgc tatatagttg
    53521 tagctgcaac aaataatatc accacaacta ttagtgatgc acacaacata agttgcagag
    53581 gtaagtgtat tgcagcataa tgtattggat gtagcacatg gtaacgatgg agtccagaaa
    53641 atacttacag gcccacattg gaagcataat gtatcaatac aaagtccacc acaaacttct
    53701 ttaaacacta ccgatggcac cagtgcctcg cgactggcag tgcaaccatt tatatcggta
    53761 acggtaaccg tatacttact gcaaggtatt gttcctgtta tggtatttgt agtagctccc
    53821 gtggaccata agtaagtata aaggcctgaa cagccgctaa cgttagcaag aatagaattg
    53881 ccacaaccac cataagtaaa gccaatgttc ataacacacg tagatgctac aatggttgtg
    53941 caacactggt ataccgtgcc attgcaatcg gtaatatcaa cacaatacgt accggcacat
    54001 aaacctgtag ctgtaggtcc tatttgtaaa gggcttacac tccatgcata cgagggactg
    54061 cataagcaag ttgtgtttac cgttatgctg ccatcgcatg agttgcacga tgaaggtggt
    54121 atggcggtga atgttacatt aggccgaatc acatttaaaa atatagagaa gccgcctgca
    54181 tccgttacgg ttaagcacat aggtgtgcat ggttgtatgc tagataaatt gatgcaaggc
    54241 aatgtggtac cattgcacca taaataggtg taaggaggag taccgccaac aacattggca
    54301 cattgaaaat cgccacaact acttgttgta aaatttacag tcatcaccga tgatggcgcc
    54361 agtattatgg atagcgatga tgtacatcca acattgtcgg taacggtaac attatacgta
    54421 cctgctccta agccggtaag gttttgattc gtgcttccgt tattccagct aaaggtataa
    54481 cctccggtgc cgccattcac acttaaggta atgctgccat tcagcgcaat tgttgatgat
    54541 ggatttaaat gggtttcggt aatagataaa gctgtattct ctgttatagt taacgaagca
    54601 ctacctgtat cgccattgaa atctgtaaca ataaccgtat aaactcctgc tgttaatgag
    54661 cctacactag cgctgcttgc accattggtc catacataag ttagcggtgg agcggcaccg
    54721 tttgtaacgg cagttgcggt accagtagca ccaccattgc aaagtacatt ggtaccgtta
    54781 accgccacaa aaaaattagg agccattaag tttgtggtga ccgtgccatt gtcacaattc
    54841 ggtgaatggt aattttcaca gttaggactt gcagcgttgt ctttcagtgc attacccgaa
    54901 aatgcattga aagtagctgc cattataaaa cagcagcaaa taataattgg cctgatacaa
    54961 gataaactaa cattgagttg cacttgtttt gaagtagttt ttttcatttt gccttttttt
    55021 agtattttat ttagtaacga attgttttat ttgattaatg tttcaaaagt attcacaaca
    55081 taagcactca gcaatcgcat tcttgtatca ggtaggtttt accttgtagt tagccacatt
    55141 tgccttgtag taagtaatgg aatgtttaaa aaaaagtgat aaacctcgaa ggaaacaaaa
    55201 gccgatatta aatttcctgc aagtgttgta taaaactttc ttttttgttt cgtgctacat
    55261 ccagtacagt accatcagtc attttcactt gcccaccatc gccacgaata tatttactca
    55321 ccttcatcat attaatcagg taactgttgt gaatacgaaa aaaagaaaaa tgctctaact
    55381 cgcgttcgta gtctttaagt ggtttcgatg ttactatttc ttttttattt tcgaaataga
    55441 aagtggtgta tacgttttcg gctttgcaat aaataatatc gttgaggtca aaaaattcta
    55501 tgccgttttt atcgctgagt ccaatttttt tgcgcgtatt gtttgggttt atattgtgca
    55561 aaagattttc cacttttagt ggtgtttgat ttttttgtgc atgttgtttg tacttttcga
    55621 acgaagcgaa aaggtcatcc ttgctatagg gttttaaaag gtaatctaat gcagaaaatt
    55681 ttatggcatc caatgcatac ttatcgaaag aggttgtaaa aacaacttcg aaatttttat
    55741 tcgccacagc tttcaataaa tcgaaaccgg tgaggggcgg catttgcaca tccaaaaaaa
    55801 gaatatcgaa agcggtttgg tttacaagtt gtattccttc tattaccgat gatgcaaatc
    55861 ctataagttc cacttctcct ttgagtgaag tgatggcttt tttgaaataa tctatatggg
    55921 ctatttcatc atcaactata atacatttta tcatattgaa gaaatggttg actgtaaatg
    55981 taaggaatta taatggcata gcttcgagaa aataattttt gcaaaaacaa aaactattag
    56041 cctgatagca aaacacaata tcttattatc aaataaccaa tatccttatt aatcttcacc
    56101 aacaaatgga atagttatag ttacccgtgt gccttcgctt tcatttgcat tgttgtaaac
    56161 atcttcggtg gtgtaatccg acttttgccg tgccgcctta ctaaggtgtt gcaaccgttc
    56221 ctttgtaagc ggaatgccat acgattttct atttgttgtt gcagtttttt cttttggccg
    56281 gccaataccg ttgtcttgca cttgtatctt tatctctttg tcaccttttg caatgtttat
    56341 aaaaatactc ccgtttttat gcaatggttt tatgccgtga ataatagcgt tttcaactat
    56401 gggttgtata agcaagggtg gaatataaat ttgatcgaca gcaatatttt catcaacatt
    56461 aattttaaat tccaaaggaa aattgaggcg cagtttttct aaagccacat aatccttcag
    56521 tacttccaaa tcatcttcga gtgtcactgt ttcctgtgta gaattgtcga gcaccttgcg
    56581 tatcaatttt gcaaatacta aaagataatc atcggccttt tgcatatcgg ctgtggaaat
    56641 aaaattttgt atagaatgaa tgcaattgaa aataaaatgc ggattcattt gtgcattgat
    56701 tgcctgcatg cggagatgca taattttact ttgggtttcg gcctcgcgcc tgcgtttata
    56761 aaacaaaaaa cttataatgg cagaaatagt aagtaacgat gcaccgccca aaatggaatt
    56821 acgaattact ttttgtcgtt gtatttctgc ttgcgcctgt gcttcctttt ttacttgttc
    56881 cagtttaagt tgttcttctt ttttatcgaa ctcgtaaccc gcttgcaatc gcataatgga
    56941 aaggcggttt tcatcattgc gcaaactatc ggtaagcact ctgtatagcg attgcatttc
    57001 gtatgcctct ttgtatctgc cttgcaaaga atatagttga tgcagatatt ttgccactcc
    57061 caatcgtgca gttaaagttc ccagaaagtc agcatttttt tgcgcttgtg taaaatacat
    57121 ttcagcatcg gcatacttct tatcttccat tgccagatta ccaaattcaa tatttacagt
    57181 atacaaccct tcggcatcgt tcaactgaga aaaaatagca tagctgcttt tataatattc
    57241 caatgcgttt gctatttcgt gcattttttc atatgtacct cctatattgc ttaacagaac
    57301 tgcttgttga cggctgtcgc caatggcgcg aaataatttt tcactttgta taaaattgaa
    57361 taaggcttca tcctttttat taagcatgtt atacacggct cccaagttat ttagcacttg
    57421 tgctatagca gtagagtcat tattttttag ttgcgagttg taactaagtt gtaaatattt
    57481 taaggcattg ttatagtcgc ccaattgatt gtaaaccgta ccaatttcgt tttgtgcttc
    57541 ggctatttta tttattaaat ttagcttttc gaaataggat aaactttgaa ggtgaaaatt
    57601 taacgccttt ggcagttgtg cctgatcgta ataatagatg ccattgaaat ataaatgtgt
    57661 ggcaaaatat ttttgcagtt cttttttttc tgcagcatta ttagttttct ttagtaatga
    57721 tgatgatagt tgccccattt ctaagttata tggaagccaa atagaatctt cttcaataag
    57781 ctcgataagc tgatttaaaa tatcaagttt atgattatcg ttttttgcat ttacaagtaa
    57841 ggattttaag gaatcaactt cggtttgctg tccacttaat acctgatagt gtagtacaag
    57901 aattatgcaa agtgaaagta gcacattatt tttccatcgc tcataaaagt tggaaattat
    57961 tttttgtacc gacactttca tttattaaga aattagaaaa tggaatgtga caaagctact
    58021 atttgtattg atacaatttc aggtatcaga aagaggaatc aagcaaaggt gctttactct
    58081 ttgtaagcat attttatttt ctatcgtaat taaaccaatt accttcataa ttgcgctcaa
    58141 ggttttcttt gctgtctata tgaatgaaga attcttccct tttgaaataa ttcatttcat
    58201 cgtatttttt taatagcgca aaatattttt cggtgagtaa taatcttgct tcattgttag
    58261 cgttttgcgt tgcttgtatg tcattgtaaa aaaagaaagt aggtgtacca tatgtgcctt
    58321 tatggatttc ccaaaagtct tccatattta attgtgattt aaggttttgg atttcttctt
    58381 ttgtaatttt ccaagtaact tcagtttttg cgatgggttc aaacgcacta aatatgatcc
    58441 agatgttttc tgttttgtac ttatccgaat tgtgtggtga aaaataattc cagatatttt
    58501 ttttaattgc cagtccttga ttaattaaag tcattttaaa ttgcgttgca atctctcgtt
    58561 gcttaacttc atcaaagcca aacttttttt taaacttttc tgcttcctca aatttttcaa
    58621 aaataatatt tagccggggt cgggtatcat tgtcaattgc atcgtaaata atattcaaag
    58681 tatttacaat gtatttttta ttgatataat cggcaagagg cataaaatca atttccattt
    58741 tggcctcacc tttcataatc aactttgtcg atgcatattc tttgtccgaa ggcgatacca
    58801 taattttatg gaaacgattt tatagaaaga aaatttgaaa gttaaactaa cgcaaacgca
    58861 ctattgcagc ttgctttggt tgtatattaa gtttcactcc ttcgtttgga ttgtatacca
    58921 ccttatcttt agtcagctcg tcattcaatt ctttagtttc ctgaggcaac gaaatttgtt
    58981 ttattacatt ttcaggaata aaaatatttc cctgatatgc gtttgtagaa aaatttaccg
    59041 atatcaaaaa ggcttcgttg gcatcgtaac gtatataact gtatacaaga ttgttggtgt
    59101 atttttctgc attagcttct tgcaggtcgt aaaaatatcc actttgtatg gccgaagatt
    59161 tttgacataa acgcaatatt ctattataaa aatttcttgt actgcgttgt tcatcattca
    59221 gcaatgcgcc atcatattta tgattattca tccatgcctg atgtgccggt actccccagt
    59281 aatcgaaaat agaagtgcgg ccatcatcac cgccaaaacc ttcggcaccg gcaccggctt
    59341 caccaacctc ctgaccaaag tatatcagca gcggcccgct tcccaaagtg gcagacaccg
    59401 ccatggcacc aaaagcattt tttgctgctt tgccaaataa cggtgacgct atacgttgct
    59461 catcatgatt ttcgagaaac ctcaccagat gattgcttat gccattagtt tcatatttcc
    59521 agcagtgtgt tatatcgttt actttgccct tgtcttctat caatctgcgc aaagcatcgt
    59581 aaattcctac cttatcgtat atataattga acttgccctg gttaatataa tttctgtatt
    59641 cggcaggatt ataaatttcg gcaatgaaaa taactgcggg atatttttgt tgtactttgc
    59701 ctatggcgta attccaaaat tcaacaggca ccatctcggc catgtcacaa cggaaaccat
    59761 ccacaccttt tgctgcccaa tacaaaagta tatcggtcat tttattccag gtgtcgggaa
    59821 tgggatcgaa atattttgtt tggttgccct gaatatcaac accataatta agttttacgg
    59881 tttcgaacca atcatcttta ctgggagtgg ggctaaaaac attgttgcct gtggctttgg
    59941 cgggatactc atcgtaaacc tgattttcat tcaatccttt ttcggtaccc atcggattat
    60001 aatctgctgg cacaataaat ttttgattga gtaaataata gaaattgttg gctgggttaa
    60061 aggcatgtcc tttattatcg ttttcgccca aatctttaac tccatttggt tttgcatcgc
    60121 taccataact tcgcgcaacg tggttgggaa caaaatcgat gatggatttt aaacctgcat
    60181 cgtgagtgcg ttttaacaaa gcttcgaatt ctgccatgcg atttttaaca tcgtttgcca
    60241 aatcgggatt tacatcataa taatctttta tagcataagg tgatccggca cgacctttca
    60301 ccacatcggc atcatcctgc gatatgccat actcgctgta atcgctcatg gtggcatgtt
    60361 ccaatgcacc ggtaaaccat acatgactta caccaaattt tttaagttcg gaaagtgctt
    60421 tttgatttat atcatcaaat ttgccacaac cattttgcgc acgggtgcca taaaaaacat
    60481 ttgatgaatt agtgttgcca aacaaccggg tcatcaattg ataaataaca agtttgttgt
    60541 ttgtgatttt ttcatcggtg caattgctat cgagaagtgg tgtcatattt tttatttcat
    60601 ttttcgaaac gctatttcca caggcattaa ataatgttac aacaagtgta atgctaatgg
    60661 caaatattat tcgctgcgaa tttatttttc ttatcatttc aattttactg ttcaagtact
    60721 ttacgtttta aattaagtat ggtgctttcg tcctgtttca gggtaactaa tttattttga
    60781 ataaacaccg aaatgttttc tccggtgatg ttatctaata taatttgatt cttatttatt
    60841 gtcaattgaa taatgtgttg acgaaaacgc actttgaaag aaaaactttg ccattgcaca
    60901 ggcaacacag gattgaagtg tagcttgtca tccttcacac gcataccgcc aaagccttct
    60961 acaatgctca tccacgtgcc ggccatggat gtggtgtggc aaccgtcttc ggtatcattg
    61021 ttatagtcat ttaaatctaa gcgggcggtg cgcaaataca tttcgtatgc cttatcggta
    61081 tcgccaatgt gtgctgctat aatagcatgt atacatggcg ataaagaact ttcgtgcact
    61141 gtgcggggtt cgtagaaatc gaaattgcgt tttatttctt ctttggtaaa gttgctttcg
    61201 aaaaaataaa tgccctgtaa aacatcggct tgctttatga aacacgatcg taaaatgcga
    61261 tcccaactcc atttttgatt taagggttta ttttcggcag gtaaatcctt taccaaaatt
    61321 tgttccttgt ccatgtagcc ttcttgttgc aaaaatattc ctaacttgtt gtgttcaggc
    61381 aagtatatat ttgcgataac gtctttccat ttatcggttt ctaatgtttc gttcaatgat
    61441 attttttcta acaaaaattt gaatttaact atgtcaatct tctttagtgc attcaaactc
    61501 tcgatagtat attgcaagca ccactgtgcc agtaagttgg tataccagtt attgtttaca
    61561 ttgttttcgt attcatttgg gcctgttacg cccagcatca catatttgtt tttctcaatg
    61621 ctccagttca ctctttggta ccagaatctg cttataccaa tcaatacttc caaaccataa
    61681 tcatagaggt aagttttgtc accggtatac ctgctataat taaaaatagc atatgcaatt
    61741 gcaccattac ggtgtatttc ttcgaatgtt atttcccatt cattgtgaca ctcttcgcca
    61801 ttcatagtaa ccatagggta gagtgcagca ccattgttga atcctaattt tgcagcattc
    61861 tctattgctt ttggtaaatg tttatagcga tacataagta agttacgtgc aactgtttcg
    61921 cttgcagttg aaagataaaa cggaatacag tatgcttctg tatcccaata agtagagcca
    61981 ccatattttt caccggtaaa tcccttaggg ccaatattaa gtcgcgcatc tttgccggtg
    62041 taagtttggt tgagttggaa aatattgaaa cgtatagctt gctgcgctgc tacatcgcct
    62101 tcaattttta tatcggcttc gtcccattta tgttgccatg cttcggcatt gtcgttcatt
    62161 aaattgaagt atcctttgtt agcggacctc ataagagagt taacacaggc attttgcaat
    62221 tctgtgtttt cgtaattctg cgatgttact atgcttacat atttacatgc tgttaacatc
    62281 tgtcctttta atatagcagt ctctatattg gcccctgcat acatagaagc agtatgatga
    62341 tttgttttgc atggtacttc tttttcatca actgcaacta tccattggca tccggtagca
    62401 acgcggaaat ttgttttgcg ggtttcagta attactatta actgattgcc gcttgcagtt
    62461 tgagaaactt cgttccagaa catttcgtca tagtttgagt cggcattgcg cacgttgcca
    62521 tcaatgaaca acgataactg tatacgtaca ttgtcgttca ccggagtgat gctgtatttt
    62581 ataacagcag tatcgtcatt tgcaatactg caaaatctct gcgattcgat atcgagtaca
    62641 acaccacttt tagtttttat ttgcatagta cgtgtgagtg taccagtttg catattgagt
    62701 gtgcggcaaa acaaaagtaa ttcgcaattg gcaatgtcta cttcatcatc gttaactttt
    62761 acatgtatgc ttttccaatc agctgcattg agcactttgg caaagtattc ggggtagccg
    62821 tttttccacc atcccacacg tgttttgtcg ggataataaa ctccagccac atagctgccc
    62881 tgcatggttt ggcccgaata tttttcttcg aacataccgc gttgcccaaa gcgtccattg
    62941 ccaatactga ataaactctc gcttatttta ttgtaagatg catgaaagct gtcttcaata
    63001 attttccact catcgtgttt taaataatcc ttcataacag aaataataaa aaattttatt
    63061 tgaaaaaatt aagaatatca ttcaaagaaa tattttcgaa ccctgccaaa ataaaatgtg
    63121 cattgggcaa tgattgttta ttgccgatac ccaccacatg aaaacctccg gcaagggcgg
    63181 cttccactcc tgccggggca tcttcgaata caatgcactg agcagggttt actttcaaat
    63241 gctgtgctac atttaaaaaa actgccggat tgggtttcga ttcttttacc gaattgccat
    63301 cggctatata atcgaactcc tgaattaaat taatattccg tagcacttcg tttgcatttt
    63361 tactggccga tgccacagca atttttatgt tgttattttt agcctccgtc aatagatttt
    63421 ttacgcccgg tagtgtatcg ctttctgtca tctgttttac ttgttccagg taccagttat
    63481 ttttaagttc ggcaaattct tttttctgat tatcattcaa ttttacattg caccatccgg
    63541 caatcagatc aagcgagcgc attctgttta tgcctttaag ttcttcgttc tgctcgtgtg
    63601 ttatagtatg gttccatgta ttggcaagtt tattccatgc taaaaaatga aaacgtgcgg
    63661 tgtctactat aacgccatcg aggtcaaata ttaatgtgtt tatattcatt actgatttta
    63721 ttttgttttc attatttgaa atatcaccga ttgatttttt tctaaactta tttttccttg
    63781 ttgttgtgtt acttttcctg tcattacatc ttttgcataa acggtatcgg gtatcatttc
    63841 ttcaaaacga tttaagctca attcttttgc agtattgttt gaattatgca ctatcataaa
    63901 tgtttgatcg tcattgaagc ggaagtatac atatattcca tctacgggta caaactgttt
    63961 taaattacct tgataaatgg taggattgtt ttttctgaaa ttgaaaagtt ttgtaataaa
    64021 ttcatgtgcc tgcttaatag tatcgggtaa attttttgct tcgaaataat ttattttatc
    64081 gccaggccac cctccgggaa aatcgttacg aacattgctc gaaggcccgt tcgaaaaatt
    64141 ttccatcaat atttctgtac cataatacat actgggaata ccgcgggtag tggccataaa
    64201 tattaatccc attttatatt tgtcgagcga cttaccacat accgaccaat accgactaag
    64261 gtcatgattg tcgacaaaca aaatgttgtt gttgggattt tgatatacgt aatcggcaca
    64321 caaagcatgg taccagcggt ttacgccttc ggtccagcca aagggttgat tgagggcatc
    64381 gttggtagca tagtataatt gaaaatcggt aacaccatcg atgttggatt tatgtaacga
    64441 tgatgtgata ttgtttttag taaaaaaact ttgattggtt attccgtgta cccatgtttc
    64501 ggcaaacata aaaaattgag ggtattcagt ttttattttt tgtgcccacc ggctttcaaa
    64561 atccaaatca ctgtatgcgt aagtatcaat ccgaaatgca tcgatatcga attcttcaat
    64621 cagccaaatg gcattttgtg tgaggtagtt ttctactaaa ggattttgtt gattcaaatc
    64681 gggcatgtgc acatcgaacc atccatcggt catttgtttt ttatcggcat cggctgcata
    64741 aatatccatg agtgtaccat ctttataagt tgtgcgcgta aacgaattcc attgatgtac
    64801 aaaatcattt gccggtaaat cttttattaa ataatgttgg ttgcctatat ggttgagcac
    64861 aatgtcgtaa atcattttca tgccaagttt gtggcatgtg tccacaagtt gtttatataa
    64921 agaatttgtg ccaaggcggg catcaatttt atattgatcg gtaaatgcgt aaccatgata
    64981 cgattcggtt ggctgattat tttcgacaac gggatttagc catagtgcat tgaatcctaa
    65041 atcttttata taattcaaat gttgcatcac gccttgcaaa tcgcccccat gacgcatgat
    65101 gagcgaatcg cggttcacat ctttgcagtt cattgttggc accacatcgt tttttgtatc
    65161 accatttgca aacctgtcgg gcatcattaa gtagattgca tcggaggtgt ttaacccctt
    65221 tttgccggca cttgaaatat tgcgctgttt tataaggtaa gggaaagtga tatttttttt
    65281 gccaccggta aactgaatat tcataacacc tggtttagca ctctcgtcaa tttttaaatc
    65341 gataaaaaga tagtttttat tttcggggcg gataacattt gtgatagtaa caccggaata
    65401 atttattgca ggggttgttg ctgaaatatt attgccatgt acgcatagtt gtaaattttg
    65461 atttataaag ccggtccacc aatgtggagg atcgatacgg tcgatggcaa ttttggccgt
    65521 tttattttgc gaaagtaaat tggtatttgc aaaaagtgat gtgacaagaa gaataaaaaa
    65581 atatttgtaa aatatatttg caacaataca acccggtcgc attattttat taatgatgga
    65641 gaaaaaattg gctccattta ttaagtagtt tactgtcata gttaatttga agaaaatgtt
    65701 gaaatgttaa ttcgattcgt atttatcaat tgcgtgttgc acatgatggc cacggaaaaa
    65761 aatttcaaga aaataaaata actctttgca gtttgggcaa ccaacttcaa tcaatttaat
    65821 aaataaaact aaaaaaaata actgcaatca cataggggga aattaggagt gcttagtgta
    65881 attgcaacac aaagttaatc tattgatcct atcgcacaac atagaatcaa aaaatgtttt
    65941 ttaggagtat tgaacattta cacaataact atttttgtaa tgggtaattt tgttagtcga
    66001 aatttcatgt tatgaaattt ctattgtagg acaaaatgtt acaaatgagc aaagtttaaa
    66061 aagtttgcgg agaaaatatt ttcaagaagg caacgctact gaagtttgta aaatttcaat
    66121 ttgctttgtt tgctgtttgc aaaaatactg atgtaataaa agcctgtttt caaattaggt
    66181 aaatctattt tcatggcggt agcggtttct gattttgcat aagttgctga aagtaccgat
    66241 tgccctaatg tattgtaaac tgccacctct acatcggtag agaggttgcc tattaccagg
    66301 atgttgatac tgtttagtaa agtattatta ctcacgagtt caaaatttac aacattggta
    66361 tcgggcgaaa tattgggagg tacacacaaa tcttttgtgg tgagaaatga cgcacgataa
    66421 tcaacaatgg ctttgttcat tctgtttgat tgacctttgg taaacatgtg catacattta
    66481 tcgaaagtat aatccatgta gttcatgtac atgtcgccat cgggtccgtt gttacacgta
    66541 acgtgtggaa aaccaggaca taaatatgcg ccatttacca cattgccaaa gttggcttgt
    66601 tcttgtgtag gggtatcgct taccaaatcg ttaccacaaa cattgtcgcc ccaaatgtgt
    66661 ataaggttga gccaatgacc tacttcgtgt gttgtggtgc gccctttgtt atatggtaaa
    66721 ctaacattgc caacattacc aaaggcttcg tagcgcacta caatgccatc ggtttcggca
    66781 gggccgtttg gaaattgcgc aaatcccaaa agattatcgc cattgaaatt tcgcaaatcg
    66841 cacacccaca tattcaaata ttgatcggca ggccatgcat tactgccacc actggtatca
    66901 aatttcacat tgttgtcgag cgaaaaatat ggctggcttg tttgcttgta aactattcca
    66961 ctggtggggt tgctatcggg gtcgagggtg gcaaggcaaa attgaatttt tgtatcgcca
    67021 attttcgatt gaaacactat gggtacgttt gtaatatcat cattctgcgc acggaaatca
    67081 ttgttcaaaa tcgtgatttg cgattctatc tgttgtggtg taatgtgtgt attggtgcca
    67141 ataggttcgc cacgatgtaa tacatgtacc acaaccggga tagtaataat agcctgagta
    67201 cgttgaatat ttgtttcatt atttgcagca tcgattagtg attttttaag ttgaggattt
    67261 ttagcataca actgctgcat atacatttcg gttgcacact tttgtggcaa tgcctggcca
    67321 taagtttttg cactttgaaa aataaaaatg attgtaatga aaataaataa aattgttgca
    67381 gaaatatttt tgtacatgat aaacattgtt gatcttaaaa attcgtcttt tgaaatataa
    67441 atgcaaattt atatttttag ttcaaatcga ataattaaac ttgattgtat gctaaataag
    67501 ttttcagcaa aatgttggtg ataaaataaa cggcagtgct gaataatttt tcgaaagaag
    67561 tagtttacag ataaaggtga tttttacgaa aaatatttct ctcagatagc acagattttt
    67621 tgctaatttt atttctcgca aatagcttag attttttaaa attctttgca cacagaaaat
    67681 acagaatatt aaaaagaatt aatgctttgg aataaaataa tctcaaccgc ttttttcaat
    67741 tttaaaatcg taacctttgt attcccattc ttcgaacaac tcgcggtcga atgccagttt
    67801 atatttacgt gaagtggcat caacataata agtagactca tcgtctttta cagcaatacg
    67861 taaaggaact tttcccggtt tggcaatcac actctgcaac aggtcatcta tccatgcacc
    67921 gtttatttcg tgcgactttc catgcactac tatacccctg caattgctgt cgccaacatc
    67981 gccaagcaat tgaatgttgt ttattttcaa ttcgaattct tcgggcttat tgtatttgtt
    68041 ttgaaccgta gcacgcacaa atacaaacat ttgttctttc aaatattttt cgaactcgag
    68101 atattttttt tcatcgaaca atggaaactc cactgcgctt gttaaatctt cgatggtaaa
    68161 aataccaaaa cgttttccgg tttttcctat acggtgattt gcttttgtaa ttatgcccgc
    68221 aaacgaaaaa tcttttttgt ataaattttt cagttcatcg ggttgcaaat cgctgcactt
    68281 atgtgtgcaa aataatttca tttcgtactt aaataaatcg agtgggtggc ccgataaata
    68341 aaatcctatc acttcgcgtt cgtgatgcag ttgttcgagt ttgctccatg gctcgcttgt
    68401 gggtaagcgt ggttcggtgc tatccgactt tgagcccaca tcgccaaaga ggcttatctg
    68461 cgaagcgttt tcagcttcct gttgttgttg tccgtatttt actgcatagt ctattcctgt
    68521 aagttcatct ttgccggcag taagatattg tgcacgatga atattcttga aactatcaaa
    68581 tgcgcccgat tttgcaagcc cttcgagtgt gcgtttattt acactacgca gatttacccg
    68641 ttttgccaaa tcgtaaatcg ataaataatt tcctcctttg tttctattct caattatgga
    68701 gtctaccgca ttttcgccta caccttttac tgcgcccaat ccaaaccgta tttgcttttg
    68761 tttatttact gtaaacttaa acgagctttc gtttacatca gggcccagta cttgtatatt
    68821 catacgcctg cattcttcca taaagaaagt aactttttca atattactta agttgtgagt
    68881 aagcactgca gccatgtact cgggtccata gtttgctttt aaatatgctg tttggtatgc
    68941 cacaaatgca taacatgttg aatgcgattt gttgaaagca tagctggcaa aggcttccca
    69001 gtcggtccat attttttcgc atgttattaa attatggcca cgcaaaccgg ctccttccat
    69061 aaactgacct ttcattttat tgaggtcctt tatgtttttc ttacccattg cttttcgcaa
    69121 attatcggca tcgcctttat cgaagccgcc caatttttgc gaaagcaaca tcacctgttc
    69181 ctgatacaca gtgatgccat aagtttcttc taagtattcc tgcatttcgg gtaagtcgta
    69241 tgttacaggt tcttgcttat gcttgcgcct gataaaactt ggaatgtatt ctataggtcc
    69301 cgggcgatag agtgcattca tggcaataag gtcttcgagt ttatcgggtt tcaactcttt
    69361 caaatatttt tgcatgccca cactttcaaa ttgaaaagta ccattggtat cgccacgctg
    69421 atacaaatcg aaagttgctt tatcatcgag cggaatagtg tcgatgtcga taacgatacc
    69481 ataattttgt tttatcaatt gtaaagcatc gcgaataatg gtaagggttt tcaatcccaa
    69541 aaaatccatt ttaataactc cggcatcttc aatttttgtt ccttcaaatt gcgataccca
    69601 caggtccgaa tctttggcaa cactcacagg aataatatcg gtaagatcac atggagcaat
    69661 aataattcct gcagcatgca caccggtgct gcgtacactt ccttcaagtt ttacagcctc
    69721 tttcaataca tcggcacgta tatcgttacc ggccattata tcgcgcagtt tttttatgtc
    69781 ttccagtgct tccgaattaa aaccatcgtg ttctttaatt cctttttcgc catccatagg
    69841 ccccagcaaa attctgttga gttcggtgcc gggtttttcg ggtaccatct tggcaagtaa
    69901 gttcgattcg tttaaaggca aatctaacac acgcgccaca tcttttatac tgcttcgcgc
    69961 tgccatggtt ccgtaagtaa taatttgtgc cacctgattg cgcccatatt tttgcaccac
    70021 ataatctatc actttttgcc ggccttcatc gtcaaagtct gtatcgatat cgggcatcga
    70081 ttttcgcttg ggatttaaaa aacgctcgaa cagtaaatcg tattttatag ggtcgatatt
    70141 ggtaatgcca atgcaatatg ccacagcact accggcagca ctaccgcgcc ccggacctac
    70201 aaacacgccc atatcgcgcc ctgcttttat gaagtcggca actataagaa agtagcccgc
    70261 aaaccccatc gattctatta cactcaattc gaaattgaga cgctcttcta tttcggttgt
    70321 tagttcatga taacgatttt ttgctccttc aaatgtgaga tgacgtatgt attgatcttg
    70381 cgttaaaaat ccatcgggaa tttgatagaa gggaagtaga atatcttttt taagattgag
    70441 tatatcgcat ttatcaacta tatgttctgt attgtcgagg gcatatggta tgtcgctaaa
    70501 tgtgtgtaac atttgctcgg tagttttgaa ataaaattca tcgttgtaaa atgcaaagcg
    70561 cgcacctttt ataaaagtat catcgtcacc aaaatctttc atcttgggtg tactttgttt
    70621 ttcgcctgtg tttacgcaca acaaaatatc gtgggcattg gcatcttcct gatttacgta
    70681 atgagagtcg ttgctgcaaa taactttcac attatacttc acactccact tcaataaaac
    70741 ttcgttcact ttattttgat cgggtatacc atgcctttga atttcgatgt aataatcttc
    70801 gccaaacaaa tcgagccacc acttaaaaag tttttcgcct tcgtcttcgc catgtcgcaa
    70861 aatggtgcgt ggtatttcgg caccgaggca acacgtagta gctatcaatc ctttatggta
    70921 ttgcagtata agcgatttat cgatgcgcgg atatttgcca tacatgcctt cgatgtagcc
    70981 cagcgaacaa agctttacaa gatttttata cccctcagca ttttttgcta aaaacaactg
    71041 atggtaacgc acatctttat cgtccttggt aaattgcttg cgcgtacgat cgtccaccac
    71101 ataaaattcg caacctacta tgggttttat aacatcgtag ttttctttat tatactttgc
    71161 tgcctcggcc acaaactcga atgcgccaaa catattacca tgatcggtaa tggcaatggc
    71221 ccgcatttta tcgcccgtag cttttttaaa aagtttgcca atagaggcag caccatcaag
    71281 caagctgaat tgagtgtgta catgtaaatg attgaatgtt ggcatgagta gacttcttgt
    71341 ataaatattt ttctttagtt gaacgtgccc ttactttttt ggtgataatt ttattttcga
    71401 atcaatacaa attaaagagt ttttattttc gaatcacatc aacaaaaata gagtaggagg
    71461 caaccaagca tcaaatgaaa ttaatatgca tgctaaatga aaataatgtt gataaaaagt
    71521 gaaaggtggt gagaagtggt tgttcaaata gcctcagttg gagttttctc caactgccat
    71581 ccatgcctaa gttggaagtt tctttaactg ctttgtgttg tggaggtcaa ttggatttta
    71641 catttcgatt gacgatggta ttttctgcgc taataccccc aaaccactga cacttcggtc
    71701 ggtgcaggcc cttacaaggg gctttccatt tctatgctga gttttcgtat tatagtcttt
    71761 taccttcgtt atcgtttcac tttgcctcag ttggagtttt ctcttagctt ggcgtagtct
    71821 ctgactacgt cactgtgtag gtcaatctct gattgacgat aactagtgta tgattttctt
    71881 gcctcagttg gagttttctc caactgcttg gtggtgtacc attgcaattg tctgaatttt
    71941 gatttaggag aagaatacac taatgtagta taacaatttt tccattttgc tcgctaacct
    72001 caccccccga cactgcggtc ggggcaggcc cgacccctct ccatttggag aggggagccc
    72061 tactcacagg ctcactgcac tccaaaaata aattgctttg ttttttttca tagttgttat
    72121 ttcaataaaa aaacgctcat gtattataga ttcatgagag tgcagattac caaatattaa
    72181 aggtgaacca atttcaaaaa aaattgaata aaataaatat tttaagcaaa cattagtt
//
LOCUS       DAORQA010000003        55814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_95_length_55814_cov_46.391847, whole genome shotgun sequence.
ACCESSION   DAORQA010000003 DAORQA010000000
VERSION     DAORQA010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 55814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55814
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95_length_55814_cov_46.391847"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PK736_00590"
     CDS             complement(<1..235)
                     /locus_tag="PK736_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31918.1"
                     /translation="MKSICVILFLFLKLSPVLSRANIQNDSAAPPIIASEYQQNIYNE
                     ETKSFNPSYNYSNLWDIDGDKKNDSIYFIGNGGV"
     gene            complement(342..1256)
                     /locus_tag="PK736_00595"
     CDS             complement(342..1256)
                     /locus_tag="PK736_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HRC31919.1"
                     /translation="MNRYLFVISVFILSSLWASSQDISVAKFEDDHATIAQMDLAICG
                     NQFQKMENGFNELYVKQEPNHQKKEECDNGYKPVTGNNNRCYLNKCWAKKSNVRVKHQ
                     GYDSSNFVYNDFYTWPIERVCIPLYKVMPVITNLNDGTFYYKIEDKEFRGTQNICKCL
                     PEFAKVSCPRGEVNINELNTGDTVWTKSKDGKKIAVPVLIANKVALSDAHLMVHIDLA
                     DGRNIQVTAGHPSFVNDKSIGDFEIGDYYDGSKIINKYEMIYSEKFTYDILPEGETGF
                     YWINGILLGSTLYNYNSLVNNINCGKNK"
     gene            complement(1521..1913)
                     /locus_tag="PK736_00600"
     CDS             complement(1521..1913)
                     /locus_tag="PK736_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31920.1"
                     /translation="MNTSITFGDYVHCRKDGENKYRDDGTEVLNMLGRLTSFVVAGKY
                     KFANGYIMKENAKLAPYIYLGAGVNNISEHWWKDKPRAQIGNYTSLNAGLGVRYNLCH
                     KFNIGYNMGYGYFILRVDRFSNESITYS"
     gene            complement(2028..2741)
                     /locus_tag="PK736_00605"
     CDS             complement(2028..2741)
                     /locus_tag="PK736_00605"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRC31921.1"
                     /translation="MSRISINTTQHVALSYEAASVGARIGAFALDALFYIAWAVLYYI
                     ITDKMGITMGDVGTFIFYLPIMFYSLICEVVFDGQSFGKMIAKIKVISTEGVPATFNN
                     YFMRWVFRLIDILMTSGGLATLVILANNKGQRFGDMAANTTVITLRKREKLNEIIAPK
                     VPEDYTVTFNEAAQLSDADIITMKKVFNYALRRANFSLIDETSDRVKTVLKIDSTMPS
                     EEFLRTIVKDYQYLMSDYE"
     gene            3530..4495
                     /locus_tag="PK736_00610"
     CDS             3530..4495
                     /locus_tag="PK736_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018618139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HRC31922.1"
                     /translation="MREAAFIKQNREKWQSYEEHATTDADELAERYVELTDDLSYANT
                     FYPKSNTALYLNNLTSTFHHTIYRNRAESWKRIIDFWRYDVPLTVHENFAKLIYAFTI
                     FTIAACIGWLSAAYDDTFVRLILGDYYVNMTIENIEAGDPMAVYSSQGSTPMFLGITM
                     NNIQVSILAFLYGLFFSLGTFYLIVSNGIMLGAFQYFFYKYNYLLVSLLTVWIHGVLE
                     ISSIIVAGAAGFCLGNSIIFPKTYSRVESFKRGTKSGLTLVAGCIPLFIIAGFLESFV
                     TRHSTVSLHVSAAIIIVSIIFIRWYFVVYPFQLYKSQNDATNRKN"
     gene            4473..5372
                     /locus_tag="PK736_00615"
     CDS             4473..5372
                     /locus_tag="PK736_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31923.1"
                     /translation="MQPTEKINLREERNLGEKLNATFGFISQNFKSLSGVIFYLCAPP
                     LIICGIGYSTFFATFFRNVTAMRYMQNTGVADIFMNSGLAFAIIAIFIMMMVAYSLVP
                     LVCYCYLKIYYQQMDIAEITANDVWKEVKPRLLPVIGWNMLLLLMIIVSCFFFIVPGI
                     YFGIVFSLFNVVYVMEEKPFGETLSRCFALIKGKWWSTFGIILVCGLVVNLIGIIFQI
                     PVWILTMASAFMKWDVADILTVTMVSNTFSIVAQMMFMSITLIGLGFQYFNLAERLDG
                     TGMRELIASIGTNKTLIPDEGEY"
     gene            5391..6251
                     /locus_tag="PK736_00620"
     CDS             5391..6251
                     /locus_tag="PK736_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31924.1"
                     /translation="MKLSIFFYDKGAQHRIHSNKEMSDILRKLKNALPFIFQKKSLFI
                     KLLSVFFILFSNGLFGDSIIDTDTVKLRPPSVERKAKFENDKDFNYHPDIDRSKNKFE
                     LWFDRMMKKISDFFESLRKKLNIDIDPAVWRWMGYIICFAVIAFIIMRLSGFNFKRLF
                     VRTSATQLPYSILEENIHEINIEQQYQNAVDAKNYRLAIRYRFLSILKTLHDNNQIEW
                     RIDKANAVYVYELSADLSAPYSVYAREFDYIWYGDFAVDAETFETIKTQMIRFEKSIS
                     LKQKTNSTSV"
     gene            6266..7465
                     /locus_tag="PK736_00625"
     CDS             6266..7465
                     /locus_tag="PK736_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31925.1"
                     /translation="MKHNKKYIFIILVMVAIYALVEYTKEKPIDWAPTYINTQKKPYA
                     TYILYDYLQDIFKGKKITTQREPVYNTAEKIDIEKGGVNYLFIGNSFAPDTLDMMSLN
                     NLVNDGCHVFIAAEYFEEQTNEQSLQQILGFKFGYDDDYKDSVKINFVSPNFKKSKPY
                     KFPYYDANKYFDLNIDSRAEVLATDSAGNPVYIRCQFGEGYYYVTTIPLAFTNYYLME
                     KGYSDYAVKCLSYMPLKYVYWDEYYKQGRAGEASMFRVMFDNPPLTWAYYITIFSLLA
                     YIIFERKRKQRIIPIIKPPVNSSVEFARSISQLYMRNMDHHNIALKKVDYFLDNIRDK
                     YRVNTNTFTNEITDELANRSGMSKQKVEELFKMINFVKYSETIDVNHLMNLNNMIKEF
                     EKTANQK"
     gene            7468..8463
                     /locus_tag="PK736_00630"
     CDS             7468..8463
                     /locus_tag="PK736_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRC31926.1"
                     /translation="MEPTQDSNVNFESRIDFSGLQANANALRNELSKVIIGQQNIVQM
                     MIVALLSDGHVLLEGVPGVAKTLMANAFAKAIDTDFGRIQFTPDLMPSDVIGTSVYHP
                     EKSNFEFRKGPVFSNIVLIDEINRAPAKTQSALFEVMEERQITYDGVTHRMTEPFMVI
                     ATQNPIEQEGTYRLPEAQLDRFLFKLIVDYPSLEEETNILNMHHQRTSFMKPASMVNA
                     IVNASDLMSYKEKMHAVHVEPKLIEYIVNIIAQTRTAKYIYLGASPRASVAVLNASKS
                     LAAISGRDFVTPDDVQAIVPHVLRHRIILTPEREMEGASNDDVIAQLIQKIDVPR"
     gene            8494..8739
                     /locus_tag="PK736_00635"
     CDS             8494..8739
                     /locus_tag="PK736_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31927.1"
                     /translation="MKKIKFRGFRFLVCGDNNLGQQDRILAHDYLVGGDTNQGQKHTI
                     IWLAGTPTKDSFGIIFLWLAGTPTKDKTKDNFNTHKK"
     gene            8740..10071
                     /locus_tag="PK736_00640"
     CDS             8740..10071
                     /locus_tag="PK736_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRC31928.1"
                     /translation="MFRIKNLFLTERLFIALGILAALFTVSFFLPWLFNAGVVLFFVL
                     ISLLIIEYAMLFAKPNMMQGRRIMAERLSNGDENDIDLYLENFYGFDIFCEVIDEIPH
                     QFQKRDVLFSITIPSSQTKILKYQLRPVKRGEYSFGAVNVFVSSVLKLVKRKYAFAND
                     KLVPVYPSYIQMRHFELLAASNRLTMLGVKKIRRIGHSMEFEQIREYVAGDDYRTLNW
                     KATARKGQWMVNSFQDERAQNVYCLIDKGRVMKMPFHNMTLLDYAINSSLVLSNIALN
                     KSDKAGIITFSNKVNNVLIADRKQFQLQKINELLYAQKTNYLESDIEALYYTVRENIT
                     QRSLIILFTNYETLRGLKRNLRYLRKMSAHHLLLVVFFENNELKELAQKTATNIQEVY
                     EQTIAEKFRFEKKQIVKELHKLGIASILTAPEQLTANTVNKYLEFKARGLI"
     gene            10245..13676
                     /locus_tag="PK736_00645"
     CDS             10245..13676
                     /locus_tag="PK736_00645"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRC31929.1"
                     /translation="MKVTFLFCILYFVCTIFSFSQTTVPSDFADVTFSSGFTSVTGFV
                     FDTSGRGYAWEKAGKVWIIDNNGTKLPQPLLNIAEEVGNWNEHGMMGFALHPDFYVNG
                     YFYVYYTVDRHYLLYYGTGNYSATTNLYNNATINRVVRYQADAATNFTTLVSNSRTVL
                     IGATKQTGIPVTTGFHDGGCLFFGADKSLIIALGDLSISAGGAADTGSYNGTYWAQAI
                     ADSILPVSHNVGSFRSQLVNSYSGKILRIDPLTGNGIAGNPFYDSGNPSSCASRVWAL
                     GLRNPFRCFLKPGTGSTDITAADPGVIIINEVGYNKKEEISVCENGGENFGWPLYEGL
                     SVQTNYMNATTKNPDAINPLGGGTCDSLFMFKTLLKQATLDPYVQWKNPCDTTQLIPS
                     NIPIFLHSRPVMDYNHSTVSTRVPSYNGYNAITLNVGAVNCNVTGLPFSGECNSGSVF
                     YDKIDFPLQYQNALFQCDFDMKWIKYYTFDNDMKLLSVNPFATSTYGIISIATNPKSG
                     GLFYINYGSQIRRIYYTLNVNNPPQAIAGSDIKFGASPLTVNFTSVDTFDPDNNPISY
                     KWIFDDGDTSSLINPQHTFNDTSAIPKKYNVKLIVTDSAGLNDTAIIPIYINCFPPVV
                     DITSIADSSLYATDNTTLINLAANVTDTIYADGLLKYEWYVSLYHNNHNHPEPADTDR
                     VTYAIVGPIGCGENYYYKVSLVVTNPLGLSATNTRYLLPDCDTPIAAFTFNNDTVCNG
                     QTIQFTNQCTGKSLQYTWTFDGGTPATSNAANPLIQYNDAGWHNVKLKISNYNGTDSV
                     ALTNAVYVDSPLPSILNGTNAQVCRNGTPTTLRASASSAVAYAWLLNNQLLQSYTSDS
                     AIIYLPGNYSCIATGANGCIDTSLQTIVTNFNSIDTNIYSNPPSGQFCAGGNAVLTTN
                     NPTNYSYQWYKAVNAIAGQTQKELTVNTGGAYKVIITDTNGCWQWTKYFNASLEPKAQ
                     ISYAGINILCEGDSMLIAALYKDGYTYQWFNYSTLIEGATEANYFVKQNGRYRVMVTS
                     SLGCTKTSNTIKALSTICYGIKTGDLYPFIAEAQPNPASNILNLNVINDKDEMLKLTI
                     YDMLGNKVYDEDRIEVINGDNTLSIDVSNIDQGVYTLVLQSIDNSAFKKVMIVRQ"
     gene            complement(13787..14188)
                     /locus_tag="PK736_00650"
     CDS             complement(13787..14188)
                     /locus_tag="PK736_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31930.1"
                     /translation="MKTQKLFLAVFTIVLLFTSCDKNTVDPNVDDRDQYIGSWTCFEK
                     GTFSGQSTYVVNVSKVGDLDSISIRNFYQLSNAFSAIALVSSNSVTIPQQTISGIKVN
                     GFGIFSSNKINLTYYCIDGSDKDTVQAVLSK"
     gene            complement(14262..15533)
                     /locus_tag="PK736_00655"
     CDS             complement(14262..15533)
                     /locus_tag="PK736_00655"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRC31931.1"
                     /translation="MKVDVLLGLQWGDEGKGKVIDVLAPQYESIARFQGGPNAGHTLE
                     FDGIKHVLHIIPSGIFHPAIKNIIGNGVVIDPIILEKEISALIKMGVDVYSNLYVSQK
                     AHLILPSHRLLDKASELAKGKEKIGSTLKGIGPAYMDKTGRNGLRVGDCALASFEQRY
                     RSLLNKHTELLKLYDYDYTAELAEMEKAWMESLQIFKKMKVIDTAYFVNDLLQQNKAV
                     LAEGAQGSLLDIDFGSYPFVTSSNTITAGACTGLGIAPGRINKVIGIFKAYCTRVGSG
                     PFPTELENETGELIRKTGNEFGATTGRARRCGWLDLPALKYACMINGVTDLVMTKSDV
                     LAMFDKIPVCISYNINGVDTVELPYDISVPITPVYQELEGWSADLTGITTQKDMPAQL
                     VSYVKFVENYLGLPVTYVSVGPDRKQMIQCS"
     gene            complement(15570..15890)
                     /locus_tag="PK736_00660"
     CDS             complement(15570..15890)
                     /locus_tag="PK736_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31932.1"
                     /translation="MNFSYDIKVGEGKCVISLIEQLVEPHQGDDLLLALESLINKGIN
                     QITLDILQFSYFNNTAIELFSEILNVSRRAGGDCLLVGLNETQSSEEVIQKLVAIFEN
                     NVSV"
     gene            complement(16215..18140)
                     /locus_tag="PK736_00665"
     CDS             complement(16215..18140)
                     /locus_tag="PK736_00665"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRC31933.1"
                     /translation="MRKILLLTGMACAMMLNQSQAQQSHRGCSTMDHLQEQMIADPTL
                     RERMNDIERETARYVETHANEKTSAITTIPVVFHVLYNTTAENISDARILEQLNVMNK
                     DFSRTNADAGNTPSAFQGISSATNVQFCLAKRDPSGAATTGIHRVSTTVTSFPSGTSM
                     KFAATGGADAWDRNSYLNFWVCNLGSGLLGFATFPGGTANIDGVVCLYSSVGGPAAPG
                     TAVPYHLGRTATHEVGHWLNLYHIWGDDGSSCSGSDQVGDTPNQTGSNFGCPSYPRTD
                     ACTAALPGVMFMNYMDYTDDGCMNAFSAGQSARIDATINGTRASLKTSQGCVPVSTGC
                     GVPAQKQVNNISQTGCAFYWSSVVGATSYKLQFRPINTTTWTTKTTTVGNYAAVGILT
                     PGTTYQYRVRAVCGTVLSAYSSIGTFTTLTGGGCVDNYEPNETGATSKTIPANTDIFA
                     LIGTSGDLDYYTLTTVAPATKLKLTLTNLVKDYNLILLNGAATTLASSKNTGTADELI
                     IYNATAAAPYRIKVLGLGLNAWSATSCYKLRVATSATNWVREDGGYATFDESDLSVSP
                     NPTNSNIALMFESQTNGNATIVVYDAMGKLAGTITNAVVEGSNIVNINLVDYKSGLYF
                     VELRNGDSTSRIKVVKQ"
     gene            complement(18752..20512)
                     /locus_tag="PK736_00670"
     CDS             complement(18752..20512)
                     /locus_tag="PK736_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC31934.1"
                     /translation="MKLLLSYLKNYKWLLLLAMVLAAINQCFSMMDPYIAGKMIDKFG
                     VHAAEIFKGNKGAWFNDIFKYVLALIGVAMVSRIAKNFQDYYTNVVIQSMGAKLYSDG
                     IAHSLELPYEVFEDQRSGETLGKLQKVRADSEKFIVSFINIVFQTLVGLVFVVVYAIN
                     LNWMIVPVFLLTAPILAITSNFLSKGIKKVSKEILGETTALAGSTTESLRNIELVKSL
                     GLQQQEINHLNTTTQKILKLELKKVRFIRSLNFIQGTTVNFLRSCFVFLLYYLIFKGE
                     IQIGQMMTMTFYSFFIFGPLQELGNVIAIYREAEASLNNFKDIMSIPVEARPANPKPL
                     GDLQSISFRNVTFKHQSASTPALTNVSFDIKLGETIAFVGPSGSGKTTLVKMLVGLYK
                     PREGELQYNNKRGDEIELTELRSKIGFVTQDTQLFAGTIKENLLFVNPTATDAEVLDA
                     LNKAACGNLLKRADKGLDTMIGEGGVKVSGGEKQRLSIARALLRKPTLIIFDEATSAL
                     DSITEEEITHTIREVSTGKKHITVLIAHRLSTIMHADTIYVLERGKIIEQGAHSELLD
                     EKGLYYAMWRQQIGEREYSF"
     gene            20752..21300
                     /locus_tag="PK736_00675"
     CDS             20752..21300
                     /locus_tag="PK736_00675"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRC31935.1"
                     /translation="MTDNKEIILNSATFNTKVKTYIFFVVLFYLFISVIGWIVIPFWV
                     LGFGQWLSNKFFHTLKCELSNKNLKFSKGLIIHIEKTIPLENIQDLSFIGGPVLRWFG
                     LTLIKVETAGGGGGHHDNNIMSMIGIIDSENFKTEILNQREKIIKEKYTFAAQPNTSI
                     SDTQLLTDIKNELIEIKMALKK"
     gene            complement(21370..22281)
                     /gene="moaCB"
                     /locus_tag="PK736_00680"
     CDS             complement(21370..22281)
                     /gene="moaCB"
                     /locus_tag="PK736_00680"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HRC31936.1"
                     /translation="MVDITNKINTHRTAIAQAIVKVSLQATIDAIKSKTVPKGDVIEA
                     SRIAGLFAAKKTADLIPDCHPLPIEFAEVRHTIEGLQINIEVEIRTIYKTGVEVEAMH
                     AASVVALTIYDMLKPIDKEIEISSIKLLKKKGGKSQYNDILNRTIKTAVVVCSDSISA
                     GTKQDFAGKAIITKLEKYTVAIDEYSIIADEMELIQAKTKSLCADKYDLIIFTGGTGL
                     SPRDVTPEALLPLFDRNIPGIAEAARSYGMQHMPYAMLSRSVAGLIGNTLVLALPGST
                     RGAQESMDALFPYLLHIFKVMEQVRHD"
     gene            complement(22454..25186)
                     /gene="fdhF"
                     /locus_tag="PK736_00685"
     CDS             complement(22454..25186)
                     /gene="fdhF"
                     /locus_tag="PK736_00685"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HRC31937.1"
                     /translation="MTMEKFAFINDKKYAIAQGDTILKFIRRYFGKDFVPTLCDAPNL
                     EPFGSCRVCSVDVALVQNGNAKAMASCHTPVIENSYIYTDSARITKLRKNIIELVLTD
                     HPLDCLTCEVNNNCELQTVAARVGIRDVRYPEGKNHLNKKKDLSHPYMTSDFSKCINC
                     FRCVRACDEVQGEFVLTMAGRGFDSHIVKSDNKSFFESDCVSCGACAQACPTSAISDV
                     FESKSVVADKKVRTICTYCGVGCNLEVAVVNDKVKSIQAPYDAEVNEGHTCLKGRFAF
                     SFYNHPERLRSPLIKRNGEFVPVSWDEAYDFITQKFTSIKNEYGPDALAGISSARCTN
                     EENYLMQKFFRAVIGTNNIDSCARVCHSPTALGLQRTFGTGAATNSVVDLKYTDCIMV
                     IGANPTDAHPVTGAKLKQFAMKKTSIVIDPRRTEIARYATYHLQLLPGTNVAMLNMLL
                     YFIVQEGIADQKFIDARTEGYEDFKKHILSLNVDELERITGVDKNLAKAAALAYANAP
                     NAMSFHGLGVTEHSQGTYTVMLIADLAMITGNIGRRGVGVNPLRGQNNVQGAADMGCQ
                     PHQGAGYYDVTNVEYHHMYENFYNAKLPIHVGWKIPQMYDAAIGGKLKAMWLMGEDNV
                     QTDPNTTHVKNALSKLDLLIVQEIFMTETAKMADVVLPASSFLEKSGTFTNGERRIQK
                     VQAVVPPIEGTKPDGQIVVDIMNRMGYAQADYDPKTMLEEISQIVPFFAGVKWDELGE
                     NGKQWPVLPDGSDTEILHTETFKRGLGKFQTATWKQSEEIVQNEKDFPYILTTNRELE
                     HYNCGTMTRRTGNVVILKDDVLMINPEDAVKHLIEDGDMVCITSPRGKIDVKAFITDE
                     VKPGILSSTFHFPEVKMNDLTSSISDSEAMCPEYKVVAVNIRKSKGKYKAAAVV"
     gene            complement(25187..26839)
                     /locus_tag="PK736_00690"
     CDS             complement(25187..26839)
                     /locus_tag="PK736_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRC31938.1"
                     /translation="MSKNLSELSGRKGLTKNLFEELGIAAAETGTPSAARMEKLCDEF
                     LVGKANVYGAVSFYDFLKPENKGKKVYVCNGSSCMTAGTQDELHKKLAPHFEANEIGE
                     MCCLGRCHENNSFHYAGKNYSGDDVLQIEEIKKGATSPIDNYNVESRGTAVLTSDYGD
                     VKSYYNIFLECLKKTPEQLLSELKDSGLRGRGGAGFSMAFKLESCRGASGDTKFIVCN
                     ADEGDPGAYSDRYIMEQRPHSLLMGMMIAGYMAGAQHGVVYIRGEYPESITIIDECIK
                     QLYEMKLLGENINGSGFTFNFKVIRAQGAYICGEETALLSSIEGQRPEVRVRPPYPTQ
                     QGLFNKPTVVNNVETLANVPYIMQHSGKAFAAIGTAKSTGTKLISLDGFFNKPGIYEV
                     DMGTPLTEAIYALGGGFREPIKAMHIGGPLGGLVPVSMIEKLTVDFDSFAREGFLLGH
                     ASVVCIPEKYPLINYIGHLFRFTAHESCGKCFPCRLGSTRGYEMVDKALESDYKIPYE
                     LMTDLLETLETGSLCALGGGLPLPIKNAMKYFNTELSPYFKK"
     gene            complement(27075..27902)
                     /gene="fdhD"
                     /locus_tag="PK736_00695"
     CDS             complement(27075..27902)
                     /gene="fdhD"
                     /locus_tag="PK736_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRC31939.1"
                     /translation="MNTTLYNGLFFDGEKFSQVHDTLAIEVALSIAVNDVPFTITMQT
                     PGNEIDLARGILFSEKIIVDKTFTPKVEIRETNSAGFITHVNVVVPPQLVMKDFAGTR
                     NVISSSSCGMCGKSEWEDLDSEKINNQQLLDVSLVANMFDQVSEKQKSFQQSGGTHAA
                     GAFTIDAQYLTMHEDIGRHNAVDKVIGYLLNNNLLDKIKCLTVSGRISYEIVSKAKSA
                     GIPFLASVSAPSSLAVDEAQKAGITLMAFCRGKKLTVYSNVEGVKNSKEVFINGLIK"
     gene            complement(27910..29577)
                     /locus_tag="PK736_00700"
     CDS             complement(27910..29577)
                     /locus_tag="PK736_00700"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009411613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRC31940.1"
                     /translation="MSDLEIAQQASILHIRDVAAKISITADDLEYYGKHKAKLPLNLI
                     DDAKIAKSKLILVSAINPTPAGEGKTTVSIGLNEGLNKLGKKSIVVLREPSLGPVFGM
                     KGGAAGGGYSQVIPMEDINLHFTGDFAAIEKANNLLAALIDNNLQNKQNNLNIDPRTI
                     KWKRVMDMNDRALRQITIGLGGTLNGIPREDGFNITPASEVMAILCMSKDIDDLKCRL
                     ANIFVGFTFDKKPIYARDLKAQGAMAILLKDAIKPNLVQTLEGNPAILHGGPFANIAQ
                     GTNTIIATKMGLSLGDYVVTEAGFGADLGAEKFMDIKCGYAGLAPNAIVLVATIRALR
                     HHGGAKKEEYNSPSIDRVIKGFLNLEKHIENCKKFGITPVVAINYFPNDSTEEIEYVQ
                     TQCEKMGAKAVVSYGFAQGGDGTTKLAEAVLDVIENGNNKFKPLYDWNMKVEDKINRI
                     ATEIYGADGVEYSSKARTQLKMINDLGYDKLPICMAKTQKSLSDKETLVGRPKGFTVN
                     VREFEFAAGAGFIIPILGDMMRMPGLPVVPASEHMDIDNNGKISGLS"
     gene            complement(29604..30053)
                     /locus_tag="PK736_00705"
     CDS             complement(29604..30053)
                     /locus_tag="PK736_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRC31941.1"
                     /translation="MNHKPKQVFKEGAISPQFIGDSIAKHSAKTEIGAHSIFLGQVRN
                     DIIDHKKVAAIDYTAYSEMAEEIFHKIREDAFEKYSLTCMHIYHSLGKVMTGEISLFV
                     FTSSTHRDAAIESCAYIVERIKSEAPVWGKEIFEDESSVWKKNNNTL"
     gene            complement(30058..31176)
                     /locus_tag="PK736_00710"
     CDS             complement(30058..31176)
                     /locus_tag="PK736_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HRC31942.1"
                     /translation="MINSERYSRQILLPHVGTHGQEKLLSSKVLVIGSGGLGCPALQY
                     LAAAGVGTIGICDFDNIELSNLHRQILFSTNDIGKPKAQIAKAKLQQLNPEINVIAHQ
                     LKLNNTNAIDLISDYDIVIDGTDNFETRYLINDACVLLKKPLVHGAVLKWQGQVAVFN
                     VRMTEQNYSTNYRDLFPQPPDENAVLSCNEAGVMGVLPGIIGLLQATETIKFIIGSGD
                     LLVNKLLTYDILNHQQQVFSIEPNPNAHIYAPQTIEQFKHFSHKLFCTYMSDAKKISP
                     KQFSEYLQNSHLEIIDVRELNEEPTINNFIHTHIPLSTISKDISSLSYTGTVITFCNH
                     GRRGQQAANILSKHFPEAIFINLEGGIMAWLILEEQQA"
     gene            complement(31176..31409)
                     /locus_tag="PK736_00715"
     CDS             complement(31176..31409)
                     /locus_tag="PK736_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRC31943.1"
                     /translation="MEIKILAFGAVADVLSSGQMELDNLISTDELRAYFEKNYPTLKS
                     LSYAIAINKKIVLGNVNFNNGDEVALLPPFSGG"
     gene            complement(31627..32196)
                     /locus_tag="PK736_00720"
     CDS             complement(31627..32196)
                     /locus_tag="PK736_00720"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HRC31944.1"
                     /translation="MNAYILAGGKSSRMGADKGLILLNEKPMIDYVIAQLKPLCKNIF
                     IVTNNSEYKKLGFECIEDEIKNIGPAGGIFSALKHTNTQHNLIVSCDMPFIRTKGIEY
                     IMMHSLDHGITIPVFENHLQPMCGVYNKNILDKWQILILKDERKLQSMVKLFLLKQLV
                     LDYNILFDANFFKNINTPEELKDAKNYFS"
     gene            complement(32220..32957)
                     /locus_tag="PK736_00725"
     CDS             complement(32220..32957)
                     /locus_tag="PK736_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRC31945.1"
                     /translation="MIVQDILFYILLFTVAMLYASVGHGGASGYLMLMALFSFAPETM
                     RSIALVLNIFVSLVAFVPYFRQGYFRFQLFWPFAIASVPLAFLGGLEHLDPVMYKRIL
                     AVLLIFPILKLLGLQFEKSKNETEKSSIILSLIIGAAIGFLSGMIGIGGGIILSPLIL
                     LLRWADIKTTACVSALFIFVNSIAGLAAISSQNFVFSNQMVVMLVVALAGGFIGSFYG
                     AQKLNSKTLQRILAIVLVVACIKLVVT"
     gene            complement(32974..34149)
                     /locus_tag="PK736_00730"
     CDS             complement(32974..34149)
                     /locus_tag="PK736_00730"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRC31946.1"
                     /translation="MISATEAKQIIADNFLAKRIARVPLLQANQCVLAQTIVAPIHTP
                     PFNQSAMDGYAFAFIDLQHSKTFSVTQKIAAGDISKIKLLPGEAARVYTGSPIPEGAD
                     TVVMQEKVIAQNSTIEIIDDKISVGLNVRLKGSQTKQGEVVLSSGILLMPPATSYIAS
                     MGIAEVEIYDKPKVSVIITGKELVQPGQSIDGGKIYESNSFGLIAALNQLNIEPLSVQ
                     IITDDELQITNAVKQALQSDIIILTGGVSVGDYDFVADALTGAGVEKIFHKVKQKPGK
                     PFYFGRKNDTLVFGLPGNPAAVLTCFYHYIVDVIADFTQRNYFAKYTLPLANNYEKKT
                     QLTHFMKGNKNENAVTVLKNQESYLMNSFAMANCIVEFDAEKEIFIAGDLVSIRMIL"
     gene            34497..35204
                     /locus_tag="PK736_00735"
     CDS             34497..35204
                     /locus_tag="PK736_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31947.1"
                     /translation="MNIKSIALATMLATGIATVNAQELKGKKYVGGNFSLGVNSENEK
                     DDKFSKDNLNFEMNVSGGYFFTNKMALGFFLKTAFSNYTNSSKTSFSDIDDKNISNQY
                     AGGVELTKFITIKNKFSFAFKNQLVFSIMNSDYNYTSDNINGNFNSITKSTTRTFGYN
                     FIPELHYFVSEKFSFSAQMGSAGINYGNTEEDDFNKQTFNGVMLENQSVTTKSTEFSG
                     DVRFSLRQFSLGLVYYF"
     gene            35365..36069
                     /locus_tag="PK736_00740"
     CDS             35365..36069
                     /locus_tag="PK736_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31948.1"
                     /translation="MKNLLRYIILGFILILGVNACKDKDDPEIPYLGLNYYPDEIGHW
                     VIYDVDSTYKYGFDLVTHHAHYQIKEVITDTFLDNEGRKTLRLERYRKDSMSYPDWTI
                     YQVWTANKTASNLERYEDNYRYVKLQFPLTRNATWNGNSKNTLNAFDYIMTDVHETET
                     VGKLLFDSVSTVLHTDDTLNLIERTYEYEKYAANAGLVYRYSYRLEQQPVQGQLNVYD
                     TTLFTIYKETVLDYKK"
     gene            36079..37782
                     /locus_tag="PK736_00745"
     CDS             36079..37782
                     /locus_tag="PK736_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRC31949.1"
                     /translation="MIKHLNKFVFIFLCISGIFCSNNLMAQDRYVLQLADKNNSPFNI
                     NNPSAFLSPRSIARRQKQNIAITTNDLPVNPSYLTAISNTGATILNYSKWLNTVTIQT
                     ADSNILNACIALPFVLSGNNVGRVAQPNEISEVSKTKFSEKYNRNIPFAEQSNSTSKV
                     AAFNYGPGFHQINMMNGDALHNSGYSGDGMMIAIMDAGFWRADSCSIFDSLFMNNQIV
                     TTWDFVLGNSYVFDYHSHGSSCLSTMAANLPGVMVGSAPHAKYILLRTEDGATENIIE
                     EYNWAAAAEFADSIGADVFTTSLGYTLFDNPAHNHTYATLDGNTAPMTIAEDLAAEKG
                     IVVINSAGNEGNSAWNYVSVPADADSNLAIGAVDSMEQYASFSGKGPTVDGRVKPDVC
                     AQGAGTYVVWPGDGSMVPGNGTSFSGPLLAGLAACLWQTHPMLSNMDIVQAIKKSASQ
                     YAAPDSLLGYGIPDFTQASMILGGIDPASLPTEDEIYALYPNPSSNDVTVMFFTTTAQ
                     TITVSVYDAIGRKVFANKMDVAPIFINEIPIKINNTSGVYNLEIINEAGTKLHKSIVR
                     Q"
     gene            complement(37785..>38273)
                     /locus_tag="PK736_00750"
     CDS             complement(37785..>38273)
                     /locus_tag="PK736_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3641 domain-containing protein"
                     /protein_id="HRC31950.1"
                     /translation="LNSVGYGKAETGLILNLVYNPSGAFLPAAQTTLERDFKLTLKRD
                     FDIDFNNLFCITNMPISRFLDYLIQSNNYENYMSRLVNAFNPAAVAGLMCRNTLSVGW
                     DGYLYDCDFNQMLGLKVENKIQHIDNFDAHELQNRNIVLNKHCYGCAAGAGSSCGGET
                     IK"
     assembly_gap    38274..38319
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<38320..38845)
                     /locus_tag="PK736_00755"
     CDS             complement(<38320..38845)
                     /locus_tag="PK736_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRC31951.1"
                     /translation="MIKSLHARHNDLATAANQIAQLNNLPEILPFEEKLKQADLFPLR
                     AQAIDIFQINVGYMCNQTCAHCHVDAGPDRTEIMTRETMQKCLDIIAANNIRAVDITG
                     GAPEMNPHFIWLVEECTKLNVHVIVRCNLTIIVANEKYKTLPSFFAKHKVEVISSLPF
                     YNASRTDKQRGDGVF"
     gene            complement(38898..39236)
                     /locus_tag="PK736_00760"
     CDS             complement(38898..39236)
                     /locus_tag="PK736_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HRC31952.1"
                     /translation="MTSNHYYNAADLAKFGNIGEFQKELADKFFSYYNDVFKDGALSA
                     REKSLIALAVAHAVQCPYCIDAYTTDSVEKGNSETMMMEAVHVAAAIRGGATLVHGVQ
                     MMNKVKEISM"
     gene            39471..40199
                     /locus_tag="PK736_00765"
     CDS             39471..40199
                     /locus_tag="PK736_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31953.1"
                     /translation="MKLKTLTIALVAILFTGLTSYGQTVNEVVDNYFKAIGGRDKVKS
                     IKTMKITGKFFMGPMEIPVESSVKKPNKVYSTATAQGMTQRMGFDGKDAWQFSPFMGD
                     TVPHKMPEENSRDMKDQADIEGPLMDYKEKGSTVELIGKEDIEGTPAYKIKILKKSGD
                     LEYYFIDAESFLLVKSVSKKSFQGKEVESETVYSDYRDVDGMKMAFSNESRQVGEEAG
                     QKFIIDKVEVNVDMNDEMFLLPTK"
     gene            40360..43440
                     /locus_tag="PK736_00770"
     CDS             40360..43440
                     /locus_tag="PK736_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRC31954.1"
                     /translation="MKKILALLIVTVCVYDAFSQTKFTNSMFGPYEARAIGPAVMGGR
                     ITAIEGLPSDARTIYVGTAAGGIWKSTTGGTIFKPIFDKYTQSIGAMAIDPQNEKTIW
                     AGTGESNMRNSVSYGAGIYKTTDAGDNWQLMGLDSTEHISKIAIDAKNSNTVYVACPG
                     PLWSDSPHRGLYKTTDGGKTWNKILFGDNKTGCADVMVDPKNPQIVYASLWTFRRTGW
                     SFESGGDKSGLFKSNDGGSTWRKIQKGFDGQMLGRICLALAPSAPNNLYAIAEAKETA
                     LYSSKDGGENWIKQSNNQNVSWRPFYFSAIIVDPIDSNRIYRPSLSLSYSNDGGQSFS
                     EASQEGGWVHSDHHALWINPKNNNQIFLGTDGGVYMSLDRGVNFVFLNNIPVSQFYHV
                     SYDLQEPYNVYGGLQDNGSWCGPTQSPNGIENKDWTNVGGGDGFWVQPDLLDPNFVYA
                     ESQGGNMTRFNKKYNEFKDIQPYPAANETKLRWNWNTPLYSNPGNPGTLYCGAQYLYR
                     TTNKGETWERISPDLTTNDPLKQKQESSGGITVDNSSAENHCTIYAIAESPLDKNNIW
                     VGTDDGNLQYTDDGGKTWNNVTKNITCVPTFTWCSSIEPSPWDKNTVYATFENHTRGD
                     FKPYIIVSTDKGKTWTNLATADVKGFAHIVRQDIVNKNLLFAGTETGMFISMDGGKAW
                     IAYTSKIPATPVRDLKINPKTNDLIIATHGRGIFIIDDLTPIRNLTQNVLDQDAAILT
                     TRPNYLSLGQFGSGFPSAGGYSGRNFTEEAVIYYYLKDKVMTGDVRLEVYDKNGVLLE
                     KIPGSKRKGINRVTWGMKLKAPKVAKGIRLDFSGFIGPLATEGEYNIKLIKGDKVTEG
                     KMLLMPLPTNPHSKEDRDLAHTTVMTLHSMCEELALMNHQVVCARDSAKALLPMAKNN
                     KALTKSLNALIAKMETQRATYIATTAGTAITGEEKLREKLSSLYVAVAYYEGRPTDSQ
                     LDRTRAFRKELDDAAAAADKIWKSDLSNLNTQLTKAGLSNIVLLSKEEFQNIKL"
     gene            complement(43454..45169)
                     /locus_tag="PK736_00775"
     CDS             complement(43454..45169)
                     /locus_tag="PK736_00775"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRC31955.1"
                     /translation="MYFQKCSSLLLLLARYLMMVVLFLGAKPMQAQWDKNYFSAPLDI
                     KLRLAGNFAEIRGSHFHSGLDFKTDSVEGKNVLATADGFIARIKVSAVGFGNAIYIQH
                     PNGLTTVYGHLRRFNDFIDSLVYFEQSKKIKFELDWYPEDDLYKVSKGEVIGYSGNTG
                     GSAGPHLHFEVRNTDTEKTINPRLFNFAIEDTIAPILSKLVVYDGNDVVDELKPRKDD
                     VGYYIRDTLSVTPRPGFGFEALDKMNNTYNSMGIYNVLFTIDGNIVWQNRLDSFDFDE
                     TRYVNAYIDYSRDYFRNITTERLYRMPNNGFSTMKSNSTERKVYLNSKEVHKALLVLE
                     DEAGNKSALTFYFVANNLKIKKVAMQGSKTFLPYSKPYKFQNGEVIFQLNENALFDNI
                     YLQYHKSKREKKLVSPVYHFNDASFPLNKSASISIRVNGISKQLQPKLVMMRMNKDGS
                     FAYIGGTLNKNYFVATSKTLGAFALAVDTVAPTIKPLFKSDSIISNSDTLKFRYAEDI
                     SGIDNYSLTNNESKLIVKHDAKYKMLYIETKYLIAAENKLTLRVEDNRNNVSTYTVTV
                     YRRQE"
     gene            45375..45887
                     /locus_tag="PK736_00780"
     CDS             45375..45887
                     /locus_tag="PK736_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRC31956.1"
                     /translation="MPAKLRLQRFGKKGHAFYHIVVADARAPRDGKYIEKLGLYNPNT
                     NPATIELNTDKALTWLHNGAQPTETVNAILSYKGVLYRKHLNEGVKKGALTQEAADAK
                     FEAWQSQKAAKIETKKSSLAIKKSDDLKNRMVAESKVKEDRLKKIAEAQLKAFEAPAA
                     EAAEEAPAAE"
     gene            46164..46688
                     /gene="rimM"
                     /locus_tag="PK736_00785"
     CDS             46164..46688
                     /gene="rimM"
                     /locus_tag="PK736_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRC31957.1"
                     /translation="MDKKNLFQLGTILGPHKTNGEISVFIESDNPSYYKDVENILVEI
                     NTMLIPHTVEKIKITGDTAYVKLAEVNNIDEATALTKCDVYLPLDQLPPLTGKHFYFH
                     EIIGFEARDLHHGFFGIVKQVYDLPKQDIISIDNNGKEVLVPVVDDFILSIDREKKQL
                     HFDLPEGLLSVYLD"
     gene            complement(47480..48847)
                     /locus_tag="PK736_00790"
     CDS             complement(47480..48847)
                     /locus_tag="PK736_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRC31958.1"
                     /translation="MHIHFIAIGGAAMHNLALAMHHKGYIVSGSDDEINEPSRSRLAK
                     YNLLPSEMGWFPQKLNAGIDAVILGMHARVDNSELLRAQELGLKIFSYPEYIYEQSKN
                     KKRIVIGGSHGKTTITSMIMHVLKNAKMDFDYLVGSQIAGFDTMVRLTDAAPFIIVEG
                     DEYLASPIDRRPKFHLYQAHTAVISGIAWDHINVFPTYEDYKKQFQIFAEQIPGDGKI
                     IYCSEDKDLCAVMDESKILAQKIKYATAKHETRNGTTYIHHGGKEYAMQIFGHHNLLN
                     MEAARNVCTDVGISDALFYELISSFTGAARRLELIGSTQKFAMYKDFAHSPSKVDATV
                     KAVKSQFEARTLIACLELHTFSSLNETFLKGYTHTLDAADVAYVYFNPHTIEHKKLKP
                     ISVEYVAQCFVKSGLKIFTDSNLLKADLMKNNFDEAVLLMMSSGNFDGWNFENVFSEI
                     QLLVV"
     gene            48992..52957
                     /locus_tag="PK736_00795"
     CDS             48992..52957
                     /locus_tag="PK736_00795"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC31959.1"
                     /translation="MKNKKLFNITLFLFAFCCCKNLHGQILLDKHRIIGGSDKDDNPL
                     VLAYSNNSYLLFWDSKSNDGDITGNHGGKDIVVTMLDSNLNTIWQRCYGGSLDESVKN
                     VYKLVSGNILILGTSNSADGDISNNIGFWDIWALKIDSAGNIVKERCYGGTTVEILNY
                     GCLMNDSDVILSSKVGVHDYDIIPGFPNDRLWKFKIDTALGDIKKSVVEYTEPAYFEQ
                     WLVGFDSTTISDRSLSIYDYYSPIRALSTTYDINLNILSHNSHYTNSLSYIPNYMKSP
                     SGIYFEFVHNFFYVGNYPNGQYWSDMDAIVYSNPNWYRTYSGAKNDIILDGVFNDSNQ
                     LFFIGNTQSIDGDFANPNHCQDNFVMRADSLGNIQNISRISDLNNCDQLSYKIYKLNS
                     KYVITCSTNKKKGNNWSKDILIAQFDEKINKLSGQIFIDQNGNNVLDLNEQKINQPIL
                     HINPSSERPQINDGNYTYYTATSGIYNLTIDSIKYYVQAPANQTAIFLSDSGEIKNKD
                     FAFQPSGIYNDLEVSITKINSSDTSKVAIFNINASNIGTSVIDSKLSFEYDFIYNVNT
                     IKTSITPDSIIGNTLYWALDSLLPLTSMNILVSFSISDSAITGDSLTVNLKGSITSQD
                     FDTVNNVSSEVFHLEPYGNGNEMIVNIDTIITNQNYDYPELEYSICFQNSNYDINKNV
                     YIKNDLQPEYFDMSTFKFVESSHPVKILVDEPTGKIWFEFNNLNLSPVYMNELLSHGF
                     IKYRIKIKDSTSLGTILINEAEVSFDQNLKQPLVSEVHILPICSSSPDSIYSSSGSHK
                     LCDNDQIYLSVSGGALSKDGNFCWYKDSCSGTPIAKGETYYTGHITQSTMYYVRAEDS
                     CGIGSCISFYVTVSPYYPILFGTPNGVSACNDSLLIYGHALASGTTNWYNSGSLITSD
                     IDSFYAKISGYYTFEFTSDSGCKSVKSTSIDIHKIKVSLTSDSIICNGTKIKPTKYFS
                     HYSWSDGSQNPYLEIDTPGTYTVTVSDNYGCSASSSTTVGTVIPSPVLELNGNCDTTV
                     CNSILLDAQNPGSSYLWNNGATTQTYLATNSGLYSVIVTALNGCTKSDSIQVDISSFP
                     PNMLGNDTTVCGTTFSLTPSFWGPSFLWSTGQTTQSISIDTSGTYSVTVTDYVGCTGM
                     DEINLIISPKPQVFFVPFVSNTICKQGGLININQGTPTGGKYYLNHMLYQNSVIDPTT
                     LPLYVNLIAYKYVDSVGCSDSVLTTFWISPCASINSMDSESSISIIPNPASDYFTVHT
                     SISNYCVKLFSAKGELVFEKSNCKNDTRVITKMYDAGLYTIQLIAERGTIALKVSVVK
                     N"
     gene            53043..53885
                     /locus_tag="PK736_00800"
     CDS             53043..53885
                     /locus_tag="PK736_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC31960.1"
                     /translation="MKLHTINTGYFKLDGGAMFGVVPKSIWQSLNPADDNNMCNWAMR
                     CLLVEDGNRLILIDNGLGDKQDKRFFSHYYLNGDDTLVKSIRNAGYGLSDITDVILTH
                     LHFDHCGGSVIYNSDRTKLQLTFPNATYWCGAEHWDLVLHPNARERASFLKENIFPIE
                     ESGHLKLVNDGAQISPSIKVMMSNGHTAGMIHPMIEYKDKTIVFVADMIPSSHHIPVP
                     YVMGYDTRPLITMDEKEIFLKTAAEKNYILFFEHDPIVECCSLKLTEKGVRKNETFAL
                     SEIL"
     gene            53942..54472
                     /locus_tag="PK736_00805"
     CDS             53942..54472
                     /locus_tag="PK736_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC31961.1"
                     /translation="MSADKRVLFNDIYTRNKGKIFKLCLGYAGDRDVANDLMQDTFVK
                     VWNHLDTFRNDAAVSTWIYRIAVNTCLGYLRTAKNKPSEALNDYIIETKQEELIDTDV
                     KVQILYKSISKLEENDRLIITMVLDDVEYAEIAAISGISEGNLRVRIHRIKIRLTEIY
                     KRTEQEMETAFMSNKN"
     gene            54477..55061
                     /locus_tag="PK736_00810"
     CDS             54477..55061
                     /locus_tag="PK736_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31962.1"
                     /translation="MEFDDIKNLWQHQAEQTLPPPENVLQKVKLHKQKILQTLLRTVI
                     QLTATFAFILLLVLTIKFTSPVTYIGIVVMLLCMSIYAYITYKHYKLLSKDFSTLPAN
                     EYISTLKQTYIARQKFLQNGTLVYVLGLSFGLLLYLIEPISFLSPFWKIFSIVATIAW
                     ILFATLYLSKRAMKKEFDRFDEMIKHLNELNSRM"
     gene            complement(55062..>55534)
                     /locus_tag="PK736_00815"
     CDS             complement(55062..>55534)
                     /locus_tag="PK736_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRC31963.1"
                     /translation="TPASTPPNQIMQLTEIGKLAEKFWMEIPKHFPFIELGNFVVMPN
                     HTHGILIIDKSNFSESTVPAVSPVTRVLPVETPNLGVSTTIGGKNEKWKPGTIGVIIN
                     QYKRICTINARKIDAGFDWQPRFHDHIIRDAKSFERIQNYIATNPQNWKGDKFY"
     assembly_gap    55535..55544
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<55545..55763)
                     /locus_tag="PK736_00820"
     CDS             complement(<55545..55763)
                     /locus_tag="PK736_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRC31964.1"
                     /translation="MPDKFKNKYRIPSARLQTWDYASEGFYFITICTKNRKHFFGEIV
                     DAPESSVASVSPVETPNLGVSTTIGGKNE"
BASE COUNT        17343 a        10932 c        10326 g        17157 t
ORIGIN      
        1 gaacaccgcc attgccaata aaataaatgg aatcgttttt tttatcgcca tctatatccc
       61 aaaggttcga atagttgtaa gaaggattga aagattttgt ttcctcgttg taaatgtttt
      121 gctggtattc ggatgcaata attgggggcg cagctgaatc attttgaata tttgctcgtg
      181 aaagaacagg tgataatttc aaaaagagaa aaagaataac acagattgat ttcataatga
      241 tttcgctttg aatatggagg aaaataactt taaacaatga taaaaccttt agtgtaaaaa
      301 gtgaaattag ttttctaaca tttacaaaac tgcttgcggc tctatttatt ttttccgcaa
      361 tttatattat ttaccaaact attataattg taaagtgtgc tgcctaaaag aattccgttt
      421 atccaataaa atcctgtttc gccttcgggc aaaatgtcgt aggtaaattt ttcggaataa
      481 atcatttcat atttattaat tattttgctt ccatcataat aatcaccgat ttcaaaatca
      541 ccaatgcttt tatcattaac aaaagatggg tggccggcag taacttgtat atttcttcca
      601 tctgccaaat caatatgcac catcaaatgt gcgtcactta atgctacttt gtttgcaatt
      661 aaaacaggta ccgcaatttt tttcccatct ttactttttg tccaaacggt gtcgcccgta
      721 tttaattcat ttatatttac ttcacctcgt ggacaagaaa ctttggcaaa ctctggcagg
      781 cacttgcaaa tattttgagt tcctctgaat tccttatctt caatcttata ataaaaagta
      841 ccatcattta aatttgtgat tacaggcatc actttgtaca ggggaataca aactctttca
      901 ataggccatg tataaaaatc attgtaaaca aaattagatg aatcgtagcc ctggtgcttt
      961 actcttacat tacttttttt tgcccaacat ttgttaagat aacaccgatt attgtttccg
     1021 gttacgggtt tgtaaccatt gtcacactct tctttctttt gatgattagg ttcttgcttt
     1081 acatacaatt cattaaaacc attctccatt ttttgaaatt gatttccaca aattgccaaa
     1141 tccatttgag caatagtagc atggtcatct tcaaactttg ccacagaaat atcttgtgat
     1201 gaagcccaaa gggaactcaa aataaaaaca gaaataacaa aaaggtatcg attcattctc
     1261 tataaatttc gatgatgatg catcaatttg aaaattgcat aaactaatat agaagatttt
     1321 tacaagttca tagttaagag aatagtacaa atatttgctt aagaattttg aacaataaaa
     1381 attaaaatct cataccaatc aaaatggcat tttgcatata catatcattc gcgccttcgc
     1441 tgcgtttgtc aataccatcg tttgtatacc gaaacaactt aggttttgga aaaacaagct
     1501 attgtagttg ttcggcacta ctaagagtac gttatacttt cattcgaaaa tctatctact
     1561 cttagtataa aatatccata gcccatgttg tatccgatat taaatttatg gcataaatta
     1621 taacgtacac caagccctgc attgagcgat gtgtagttgc caatttgcgc acgcggtttg
     1681 tctttccacc aatgttccga aatattattt acacctgcac ccaaataaat atagggcgcc
     1741 agctttgcat tttctttcat tatatatccg ttggcaaatt tgtatttacc tgcaaccaca
     1801 aacgaagtta agcgcccaag catattcaac acttcggtgc catcgtcacg atatttattt
     1861 tcgccatcct ttctgcaatg aacataatcg ccaaaggtta ttgatgtatt caaatcgtaa
     1921 gctgcattta aataacgact gagtaactaa tttaaacttc aaactaaaaa cttcaaacta
     1981 aaaacttcaa actaaaaact tcaaactaga aacttcaaac ttcaaactta ttcataatcg
     2041 ctcatcaagt actgataatc ttttacaatg gttcgtaaaa attcttcgct gggcattgtg
     2101 ctatctattt tcaaaacggt ttttactctg tcactcgttt catctatgag gctaaagttg
     2161 gctcgtctga gtgcatagtt aaaaactttt ttcatggtga taatatcggc atccgaaagt
     2221 tgtgcggctt cgttaaaagt aacggtataa tcttcgggca ctttgggagc aattatttcg
     2281 ttgagctttt cgcgcttgcg caatgttatt acagttgtgt ttgcagccat atcgccaaaa
     2341 cgttgccctt tattatttgc caaaatcaca agcgttgcaa gcccgcccga agtcattaaa
     2401 atatcgatga ggcgaaaaac ccaacgcata aaataattat tgaatgtagc aggtacgccc
     2461 tcggtgctta tcacttttat ttttgctatc attttaccaa acgattgacc atcgaaaacc
     2521 acttcgcaaa taaggctgta aaacatgatg ggtaaataaa aaataaatgt tcctacatcg
     2581 cccattgtaa tgcccatttt atcggtgatg atataataca aaacggccca tgcaatataa
     2641 aaaagcgcat cgagggcaaa ggctccaatg cgtgcgccaa cacttgccgc ttcgtaactc
     2701 agggcaacat gttgtgtggt gttaattgat attcggctca ttcaataaaa taaaagtaca
     2761 tagaggcagc aaagaaaaca taatttttaa tcgaaaggtt gcgaaggttg gggaaggttg
     2821 cgaaggttgg ggaagttggg aaagttgtga aagttcaaga gtttaagagt ttaacagttt
     2881 aatagttacg aaaatttcct tgaattaaag ccccttgtag gggttggggt gtatttttca
     2941 agagtttaat agttacgaaa atttccttga attaaagccc cttgtaggag cctgccccga
     3001 ccgcagtgtc gaggttgggg tgtatttttc aaaagtttat tagttgcgaa aattaccttg
     3061 aattaaagcc ccttgcagga gcctgccccg accgcagtgt cgaggttggg gtgtattttt
     3121 caaaagttta atagttacga aaatttcctt gaattaaagc cccttgtagg agcctgcccc
     3181 gaccgaagtg tcagggttgg ggttgtattt ttcaaaattt aatagttgcg aaaacctcct
     3241 tgatttaaac ccccctagta gaagcctgcc ccgaccgaag tgtcgatgtt gggtgtgttt
     3301 ttcaagagtt taaagaattt gcaaaaagtt tgcacgacct actaccaaaa ctgaaaagtt
     3361 tctatagctg aacttaacct tgttgttgca ccttttggta tttaagttcc cttttgatca
     3421 tcccagtgat caaaacgttc gttgttaatt ccgcgtcaaa atcttatcat tcacattaat
     3481 aaataaaata ttagtttagc ccccattaca aattagagaa aataacaaca tgcgtgaggc
     3541 agcatttata aaacaaaatc gtgaaaaatg gcagagctac gaggagcatg ccactaccga
     3601 tgccgatgaa cttgccgagc gatatgttga gcttaccgat gatttatcgt atgccaatac
     3661 attttatccc aagagcaata cagcactgta tttaaataat cttaccagca catttcatca
     3721 tactatatat cgcaaccgtg ccgaatcgtg gaaacgcatt attgattttt ggcgctatga
     3781 tgtgccgctc actgttcacg aaaattttgc gaaactgata tacgccttta caatttttac
     3841 aatagcggct tgcataggat ggttatcggc tgcatatgac gacacttttg ttcgccttat
     3901 acttggcgac tattatgtaa acatgaccat agaaaatatt gaagcaggcg acccaatggc
     3961 agtttattca tcgcaaggat ccactcctat gtttttgggt atcaccatga ataatataca
     4021 ggtttcgata ttggcatttt tgtacggatt gtttttttcg ctgggcacat tttatcttat
     4081 tgtttcaaat ggaattatgc ttggggcgtt tcaatatttt ttttacaaat acaattattt
     4141 actagtatcg ttgctcaccg tttggataca tggtgtactc gaaatatcat ctatcattgt
     4201 agccggtgct gcaggttttt gtttgggcaa cagtatcata tttccaaaaa catacagccg
     4261 tgtagaatcg tttaaacgcg gcaccaaaag cggattgaca cttgtggccg ggtgcattcc
     4321 actatttatc attgcgggtt ttttagaaag ttttgttacg cgacactcta ctgtttcgtt
     4381 gcatgtaagc gctgcaataa ttattgtgtc cattattttt atccgctggt attttgtagt
     4441 atatccattt caattatata aatcgcaaaa cgatgcaacc aaccgaaaaa attaatttac
     4501 gcgaagagcg aaatttaggc gaaaaattaa atgccacttt tggatttatt tctcaaaact
     4561 tcaaatcgct atcgggtgtt atattctatt tatgcgcacc gccacttatt atttgtggta
     4621 ttggttacag cacctttttc gccacttttt ttcgcaatgt aactgcaatg cgctatatgc
     4681 aaaacacagg cgttgccgat atttttatga actccggttt ggcttttgcc atcattgcca
     4741 tttttattat gatgatggta gcatactcac tggtgccttt ggtgtgttat tgttatttaa
     4801 aaatttatta ccagcaaatg gacattgctg aaattactgc taacgatgtt tggaaagaag
     4861 tgaagccccg cttattacct gtaataggat ggaatatgct tttgctctta atgattattg
     4921 tatcatgttt cttttttatt gtaccgggta tttattttgg aattgttttt tcgttgttca
     4981 atgtggttta tgttatggaa gaaaaacctt ttggcgaaac gctttcgcgt tgctttgcgt
     5041 taataaaagg taaatggtgg tcaacatttg gaattatact ggtatgtggc cttgtggtaa
     5101 atttgatagg catcattttt caaatacctg tttggattct caccatggca agcgcattta
     5161 tgaaatggga tgtagcggat atactcaccg ttacaatggt gagcaatact tttagcattg
     5221 tagcgcaaat gatgtttatg agcataacac ttattggttt gggctttcaa tatttcaacc
     5281 ttgccgagcg attggacggt accggcatgc gcgaactcat agcatctatt ggcaccaaca
     5341 aaaccttaat acccgatgaa ggtgaatact aaaatagcgt gtaacgattg atgaaacttt
     5401 caattttttt ttatgacaaa ggtgctcagc atcgcattca ttctaataaa gaaatgagtg
     5461 acattttgcg taagctaaaa aacgctttgc catttatttt ccagaaaaaa agtttattca
     5521 taaaattatt gtcggtattt tttattctat tttctaatgg tctttttggt gattcaatta
     5581 tcgataccga tacggttaaa cttcgcccac catccgttga aagaaaagcc aaattcgaaa
     5641 acgacaaaga ttttaattac caccccgata ttgataggag caaaaataaa ttcgaattgt
     5701 ggttcgatag gatgatgaaa aaaatatccg acttttttga aagcttacgt aaaaaattaa
     5761 acatcgatat agacccggcc gtgtggcggt ggatgggcta cattatttgc tttgccgtga
     5821 ttgcatttat cattatgcgg ttgtcgggtt ttaatttcaa aagattattt gtgcgtactt
     5881 cagctacaca gttgccttat agcatactcg aggaaaatat tcacgaaata aatattgagc
     5941 agcaatatca aaatgctgtc gatgccaaaa actatcgcct tgctattcgt taccgtttct
     6001 tgagtatttt aaaaacgctg catgataata atcagattga atggcgaatt gacaaagcaa
     6061 atgctgtgta tgtatatgaa ttgagtgctg acttgagtgc accctattcg gtttatgcac
     6121 gcgaattcga ttatatctgg tatggggatt ttgctgttga tgccgaaaca tttgaaacga
     6181 taaaaacaca aatgattcgt ttcgaaaaat ctatttcctt aaaacaaaaa acaaattcaa
     6241 cttcggtgta aggccattaa aaattgtgaa gcacaacaaa aaatatatct ttattatact
     6301 ggtgatggtg gccatttatg ccttagtgga atataccaag gaaaagccca tcgactgggc
     6361 acccacgtat atcaatacgc agaaaaagcc ttacgccaca tacattcttt acgattattt
     6421 gcaggatata tttaaaggaa aaaaaattac cacacagcgt gaaccggttt ataatactgc
     6481 cgaaaaaata gacatagaaa aaggtggagt aaattattta tttattggca atagctttgc
     6541 gcccgatact ttggacatga tgtcgctcaa caatttggta aatgatggat gtcacgtttt
     6601 tattgctgcc gaatattttg aagaacaaac aaacgagcaa agtctgcaac agattttagg
     6661 gtttaaattc ggttatgacg atgactataa agactcggta aaaataaatt ttgtttcgcc
     6721 caatttcaaa aaaagcaaac catacaaatt tccatactac gatgcgaata agtatttcga
     6781 tttgaatatt gactcgcgtg ccgaagtgct tgccaccgac tctgcaggta accctgtata
     6841 tatacgttgt cagtttggcg aaggttatta ttatgtaact actattcctt tggcatttac
     6901 aaactattac ctgatggaaa aaggatatag cgactatgcg gtgaagtgcc tttcatatat
     6961 gccgctaaag tatgtgtatt gggacgaata ctacaaacaa ggcagggcag gtgaagcaag
     7021 tatgtttagg gtaatgttcg acaatcctcc gttaacctgg gcttactata ttaccatttt
     7081 ttctttactg gcatatatta ttttcgagcg caaaaggaag caacgcataa ttcccattat
     7141 aaaaccaccg gtaaattctt cagtagaatt tgcgcgcagc atcagtcaat tgtatatgcg
     7201 aaatatggac catcataata tcgcattaaa aaaagtggat tattttctcg ataacattcg
     7261 tgacaaatac cgggtgaata caaatacatt tacaaacgaa ataactgatg aacttgccaa
     7321 tcgcagtggc atgagcaaac agaaagtaga agagctattc aaaatgataa attttgtaaa
     7381 gtatagcgaa acaatagatg taaatcattt aatgaatctg aataatatga taaaagaatt
     7441 cgaaaaaaca gcaaatcaaa aataaatatg gagccaacac aagacagtaa tgtaaacttt
     7501 gaaagccgca tcgattttag tggattgcaa gccaacgcaa acgccttgcg caacgaacta
     7561 tccaaagtaa ttatcggcca gcaaaacatt gtacaaatga tgatagtggc attgctatcc
     7621 gatgggcatg tgctgctcga aggtgtgccc ggagtagcca aaacgcttat ggcaaatgct
     7681 tttgcaaaag ctatcgatac cgattttgga cgtatacaat ttacacccga ccttatgcca
     7741 tccgatgtga ttggcacttc ggtttatcac cccgaaaaat caaatttcga attccgtaaa
     7801 ggccctgtat tttcaaatat cgttttgata gatgaaataa accgtgctcc ggcaaaaaca
     7861 caatcggccc ttttcgaagt gatggaagaa cgacaaataa cttatgatgg cgttacgcat
     7921 agaatgacag aaccattcat ggtaattgcc acacaaaatc ctatcgaaca agaaggaaca
     7981 taccgcttac ccgaggcaca actcgacaga tttttattta aacttattgt tgactaccca
     8041 tcacttgaag aagaaacaaa tattcttaac atgcatcacc agcgcacatc atttatgaaa
     8101 cctgcatcga tggtaaatgc cattgtaaat gcaagcgatt tgatgagcta caaagaaaaa
     8161 atgcatgccg tgcacgtaga gccaaagctg atagaataca ttgtaaacat tattgcccaa
     8221 acacgtacgg caaaatatat ttatctgggc gcttctccgc gagcctctgt tgcagtgctt
     8281 aatgcaagta aatcacttgc agcaatcagt ggccgcgatt ttgtaactcc cgatgatgtg
     8341 caggccattg tgccacacgt attgcgtcat cgcattatac tcacacccga gcgcgaaatg
     8401 gaaggcgcaa gcaacgatga tgtgatagca caactaattc aaaaaataga tgttccccga
     8461 taattttata ggacggtaaa atacctggat actttgaaga aaataaaatt tcgtggtttt
     8521 agatttttgg tttgcgggga caacaacctt ggacaacagg atagaatatt agcacacgat
     8581 tatttggttg gcggggacac caaccaagga cagaagcaca cgattatttg gttggcgggg
     8641 acgccaacca aggacagttt cgggattata tttttatggt tggcggggac gccaaccaag
     8701 gacaaaacca aggacaattt taatacacac aaaaaataaa tgtttcgaat aaaaaattta
     8761 ttcctcaccg aaaggttgtt cattgctttg ggcattttag cagcactatt cactgtgtcg
     8821 ttttttttgc cgtggctttt taatgccggt gtggttttat tttttgtttt aatttcactt
     8881 cttatcattg aatatgccat gctgtttgcc aaaccaaata tgatgcaggg ccgaagaata
     8941 atggccgaac gattatcgaa tggtgatgaa aatgatattg atctttatct cgaaaatttt
     9001 tatggcttcg atattttttg cgaagtgatt gatgaaatac cacatcagtt tcaaaaacgc
     9061 gatgtgttgt ttagtattac aattccatca tcgcaaacca aaatattaaa gtatcaattg
     9121 cgaccggtaa agcgtggcga atattcgttt ggtgctgtaa atgtttttgt atcatccgta
     9181 ttaaaattag taaaacgtaa gtacgcattt gctaacgata agttggtgcc tgtttatcca
     9241 tcgtatatcc aaatgcgcca cttcgaattg ctggcagcaa gcaacaggtt aacaatgctg
     9301 ggcgtaaaaa aaatacgcag aataggccac agcatggagt ttgagcagat acgtgagtat
     9361 gtggccggtg acgattaccg tacacttaac tggaaagcga cagcacgcaa agggcagtgg
     9421 atggtaaata gttttcagga tgaacgtgcc caaaatgtat attgccttat agataaagga
     9481 cgtgtgatga agatgccttt ccataatatg accctgctcg actacgccat caatagttca
     9541 cttgtgctga gcaatatagc attgaacaaa agtgataaag ccggcataat taccttcagc
     9601 aataaagtaa ataatgtgtt gattgccgat cgtaaacaat ttcaattgca aaaaataaat
     9661 gaattgttgt atgcccaaaa aaccaattat ttagaatcgg atatcgaagc attgtactat
     9721 actgtgcgcg aaaatattac acaacgcagc ctcattattt tgtttactaa ttacgaaacc
     9781 ttgcgcggac tgaaacgcaa tttgcgctat ttgcgaaaaa tgagtgccca ccatttattg
     9841 cttgttgtgt ttttcgaaaa caacgaactt aaagaacttg cacaaaaaac tgctaccaac
     9901 attcaggaag tatacgagca aaccattgcc gaaaaattcc gcttcgaaaa aaaacaaatt
     9961 gtaaaagaat tgcacaaact tggtattgcc agtatactta cagcacccga gcaacttaca
    10021 gcaaatacgg taaataaata cctcgaattt aaggcgcgtg gtttgatatg atttctgttt
    10081 acttaaaact gataaataat cctactgata tcagcattta ttaaaatgga ttgcttttca
    10141 gttgccagaa aaaaactgtt gattcttttc tgcaaaaatt gaatcataat aatattctac
    10201 tgaagtatca ttacctttgt agcttaattg tattaaatat ttcgatgaag gttacttttc
    10261 tattttgtat attatacttt gtttgcacta ttttttcatt tagccaaacc accgttccat
    10321 cggattttgc tgacgttaca ttttcgagtg gttttacaag tgttacgggc tttgtttttg
    10381 atacttccgg acgtggctat gcctgggaaa aggccggtaa ggtttggata attgataaca
    10441 atggcactaa attgcctcag ccattactca atattgccga agaagttggc aactggaacg
    10501 agcatggaat gatgggtttt gcactccatc ccgactttta tgtcaatgga tatttttacg
    10561 tatactatac tgtcgacagg cattatcttt tgtattatgg caccggaaat tatagcgcta
    10621 caactaacct ctacaacaat gcaactatca atcgtgtggt gcggtatcag gccgatgcgg
    10681 caacaaattt tacaacattg gtttcaaata gccgaaccgt tttaataggg gcaacaaagc
    10741 aaacaggtat accggtaacc actggttttc acgatggagg atgtttgttt tttggtgccg
    10801 ataaatcgtt aattattgca ttgggcgatt tatcaatttc ggctggaggt gcagccgata
    10861 ccggttcata caatggaacg tattgggcac aagccatagc tgattctata ttaccagttt
    10921 cgcataatgt gggttcattt cgttcacagt tggttaattc ttacagcggc aaaatattgc
    10981 gcatcgatcc tttgacggga aatggcattg ccggtaatcc attttacgat agtggaaatc
    11041 catcatcctg tgcttctcgc gtttgggcat taggtctgcg taatccattc cgttgctttt
    11101 taaaacccgg caccggaagc acagacatta ctgctgccga ccccggtgtg attattatta
    11161 acgaggttgg atacaataaa aaagaagaga taagtgtatg tgaaaatggt ggtgaaaatt
    11221 ttggttggcc attgtatgaa gggctttcgg tacagacaaa ttatatgaac gccacaacaa
    11281 aaaatcccga tgcaataaat ccacttggcg ggggaacttg cgattcgcta tttatgttta
    11341 agactctatt aaagcaggca acattagacc cctatgtgca atggaaaaac ccttgcgata
    11401 caactcaact aattccttct aacataccta tatttttaca cagccgcccg gtgatggatt
    11461 ataaccattc aacagtttct acacgagtac catcctacaa tggctacaat gctatcacct
    11521 taaatgtagg tgcagttaat tgcaatgtga caggattgcc attcagtggc gagtgtaatt
    11581 caggaagtgt tttttacgat aaaattgact ttcctttgca atatcaaaat gcgttgtttc
    11641 aatgcgattt cgatatgaaa tggataaagt actacacctt cgataacgac atgaaactat
    11701 tatcggtaaa cccttttgct acctccactt acggcattat aagcatcgct acaaatccga
    11761 aatcaggcgg gttgttttac atcaattacg gttcacaaat aagaaggatt tactatacgc
    11821 ttaatgttaa caatcctcca caggctattg ccggttcaga cattaaattt ggtgcaagtc
    11881 cattaacagt aaatttcaca tcagtggata ctttcgatcc cgataacaat ccaatctctt
    11941 acaaatggat atttgacgat ggtgacactt cctcacttat aaatcctcaa catactttta
    12001 atgatacctc tgcaatcccg aaaaaatata acgtcaaact tattgtaacc gatagcgcag
    12061 gccttaatga tacggctatt attcccattt acatcaattg ctttcctcct gttgttgaca
    12121 ttacaagtat tgccgacagc agtctttatg ctactgacaa cactacgttg attaatcttg
    12181 cggccaatgt aactgacact atatacgcag atggtttgtt aaaatacgaa tggtatgtaa
    12241 gcctgtatca caacaaccac aaccatcccg aaccagcaga tactgacaga gttacctatg
    12301 ctattgtagg ccccataggg tgtggcgaaa actattacta taaagtttcg ttagttgtca
    12361 ccaatccatt gggattatcg gctactaata cccgatacct cctgcccgat tgcgatacgc
    12421 ccattgcagc atttactttt aacaacgata cagtttgtaa tggacaaacc attcaattta
    12481 ccaatcaatg taccggcaag agtttgcaat atacctggac atttgatggc ggcactcctg
    12541 caacttctaa tgctgcaaac ccattgatac aatacaacga tgcaggatgg cataatgtaa
    12601 aactgaaaat atcaaattac aatggtaccg attctgtcgc gctaaccaat gctgtatacg
    12661 tagattcacc cttgccatct atactcaatg gaacaaatgc gcaagtatgc agaaacggta
    12721 ctccaacaac attaagagca tctgcctcaa gcgcagtggc atatgcatgg ttgttgaaca
    12781 atcagctatt acagtcttat acaagcgata gtgccattat ttatttaccg ggaaattatt
    12841 cttgcattgc aaccggtgca aatggctgta tagatacatc gctgcaaact attgtaacaa
    12901 attttaacag cattgataca aatatttatt cgaaccctcc cagcggacaa ttttgtgccg
    12961 gaggaaatgc agtacttaca accaataacc ccacaaatta tagttaccaa tggtacaaag
    13021 ccgtaaatgc tattgcagga caaacacaaa aagaattaac ggtaaatact ggtggtgctt
    13081 acaaggtaat tataacagat accaacggtt gttggcaatg gacaaaatat tttaatgcct
    13141 cgctcgaacc caaagcacaa ataagctatg ccggcataaa tattttatgt gaaggcgata
    13201 gtatgctgat tgctgcatta tacaaggatg gatatacgta ccagtggttt aattattcaa
    13261 cattgattga gggcgctacc gaagcaaatt actttgtaaa acaaaatgga cgataccggg
    13321 tgatggtaac cagtagcctt ggttgcacca aaacctcaaa taccataaaa gcactttcca
    13381 caatttgcta tggaataaaa acaggtgacc tctacccttt tattgcagaa gcacagccca
    13441 atcccgcatc aaatattttg aacctgaatg ttataaatga taaagatgag atgctaaagc
    13501 ttacaattta tgatatgctt ggtaacaagg tttatgatga agacaggatt gaagtgataa
    13561 atggagacaa tactttatct atagatgtga gcaatatcga tcaaggtgtt tatacgcttg
    13621 ttttgcaatc tattgataat agcgcattta aaaaagtgat gatagttcgg caataaatta
    13681 ttgaccaacc tcactaaaag attttttttg ctaagccaat tttaaaatgt gctgcaacta
    13741 aaatattcaa gagaaaaatt gcttgttaat tgtgatagcg caaaaactat ttcgacaata
    13801 cagcttgcac ggtatcttta tcgctgccat caatgcaata ataagtgagg tttattttgt
    13861 ttgaagaaaa aattccaaat ccatttactt taatacccga aatggtttgt tgtggaatgg
    13921 taacactatt tgatgataca agtgcaattg cagaaaaggc atttgaaagt tgataaaaat
    13981 ttctgataga gatggaatcc aaatctccca ctttcgaaac atttactaca taagtacttt
    14041 gtccactaaa ggtgcctttt tcgaaacatg tccaactgcc tatatattga tctcgatcat
    14101 ccacattggg atcaacggtg tttttatcgc aacttgtaaa aagtaagaca atggtaaata
    14161 cggctaaaaa taatttttgt gttttcattt tttttatttg ctgatgatta ttgaaaccaa
    14221 atattgtgct taataaaaaa ggcttcatgt accaatcacg cttagctgca ctgtatcatc
    14281 tgttttctat cggggccaac acttacgtaa gttacaggta gaccgagata attttcaaca
    14341 aactttacat aagatactaa ttgtgcgggc atgtcttttt gggtagtgat acctgttagg
    14401 tcagcactcc atccttcaag ttcctgatat acaggagtta ttggaacact tatgtcataa
    14461 ggcagttcaa cagtatcaac accattaata ttataagaaa tacaaacagg aattttatcg
    14521 aacatggcaa gcacatccga ttttgtcatc accaaatctg tcacaccatt tatcatacag
    14581 gcatatttaa gtgcaggcaa atcgagccag ccacatctgc gggcacgacc ggtagtagcc
    14641 ccaaattcgt ttcctgtttt gcgaatcaat tctccggttt cgttttccag ttcggtggga
    14701 aacgggccgc ttcccacacg ggtacaataa gctttgaaaa tgccaatcac tttattgatg
    14761 cgtcccggtg caatgccaag tcccgtgcat gctccggcag tgatggtatt acttgatgtt
    14821 acaaaagggt aactaccaaa atctatgtcg agcaacgacc cttgtgcacc ttcggcaagt
    14881 acagctttgt tttgttgcaa taaatcattt acaaaataag cagtatcaat cactttcatt
    14941 tttttgaata tttgcaaact ctccatccat gctttttcca tttcggcaag ttcagcagtg
    15001 taatcataat catacaactt caacagctcg gtgtgcttat tcagcaacga acgatatcgt
    15061 tgttcgaacg aagccaaggc acagtcgcca acacgtaaac cgttacgccc tgttttatcc
    15121 atgtatgccg ggcctatgcc tttaagagta ctgccaattt tttctttccc ctttgcaagt
    15181 tccgaagcct tatccagcaa cctatgcgaa ggcaatatta aatgtgcttt ttgtgataca
    15241 tataagttgc tataaacatc aactcccatt tttataagag ctgatatttc tttttcgaga
    15301 attataggat ctattacaac accattacca ataatatttt ttatagcagg atgaaaaatt
    15361 cccgaaggta tgatatgcag cacatgctta attccatcaa actccaatgt atgcccggca
    15421 ttgggaccac cttgaaaacg tgcaattgat tcgtattgag gtgccaacac atctatcact
    15481 tttcctttgc cttcatcgcc ccattgaaga cctaacaata catctacttt catttagtta
    15541 ttactaatta ttattattaa aatattttat taaacagaaa cattgttttc aaaaatagca
    15601 acaagttttt gaatcacctc ttcactactt tgagtttcat ttaaacctac caataaacaa
    15661 tcgccaccgg ccctgcgcga aacgtttaat atctcggaaa aaagttcgat agccgtattg
    15721 ttaaaatagc taaactgcaa aatatccaaa gtgatttggt tgatgccttt attaataagc
    15781 gattctaagg ccaataacaa atcatcaccc tgatgaggct caaccaattg ttctattaaa
    15841 ctaattacac acttaccttc accaactttg atatcgtaac taaaattcat atttataatt
    15901 ttgttcaaat gaaatgataa ttctgcaaaa gcaacattat gtttaaaaaa aaattaatga
    15961 taccatagtt tttgcaaagg ctacagttcc aaataatcaa aatatggtat tctttttata
    16021 ccaaaattca gcaggttttt gaaaaaaaac tgctaacttc tggtaatgcc gagcaacatc
    16081 aaaagaaaaa tttcccaaaa cctaagttgt ttcggtataa cgcaaaaaag ccgcaaaaat
    16141 taaaaacttt tcgcttttaa tccttgcggc tttagttcag ccggagggta aattaacctc
    16201 tcctcatttg aatattattg cttaacaact ttaattctgc ttgttgaatc accattgcgc
    16261 aattcaacga aatataatcc tgatttgtaa tcaacaagat taatattcac aatattggaa
    16321 ccttccacca cagcatttgt aattgtacct gcaagcttac ccattgcatc gtatacaaca
    16381 atagttgcat tgccattggt ttggctttca aacatcaaag caatatttga attggtaggg
    16441 tttggcgaaa cagataaatc actttcgtca aaagtagcat atcctccatc ttctcttacc
    16501 caattggtag cactggtagc aacacgcagt ttgtagcaac ttgtggcact ccacgcattt
    16561 aggcccaatc cgagtacttt aatacggtaa ggtgcagccg ctgtagcatt ataaataata
    16621 agctcatctg ctgtacctgt gttttttgac gaagccaagg ttgtagcagc gccattcaat
    16681 aaaataagat tataatcttt taccaagttg gtcaatgtta atttcaactt tgttgccggt
    16741 gcaacagtgg taagtgtata ataatctaaa tcgcccgaag tgcctatcaa tgcgaatatg
    16801 tcggtatttg caggaattgt ttttgatgtt gcacctgttt cattaggctc ataattatca
    16861 acacatccgc caccagttaa agttgtaaag gttcctattg aagaataagc tgagagtaca
    16921 gtaccgcata cagctcgcac gcgatactgg taagtagtgc caggtgttaa aatacctact
    16981 gccgcatagt taccaacagt tgtggtttta gttgtccacg tggtggtgtt aataggtcgg
    17041 aattgaagtt tatagctagt tgcgcctacc acactcgacc aataaaaggc acaacctgtt
    17101 tgcgaaatat tatttacttg tttttgcgca ggcacaccac aaccagttga taccggtacg
    17161 caaccttgcg atgtttttaa tgatgcacgt gttccattta tggtggcatc tatacgagca
    17221 ctttgtccgg cactaaatgc attcatacag ccatcgtcag tgtagtccat gtagttcata
    17281 aacataactc cgggtaatgc tgctgtgcaa gcatcggtgc gagggtaaga aggacaacca
    17341 aaatttgagc ctgtttgatt cggagtatcg ccaacttgat cacttcctga gcatgaacta
    17401 ccatcatcac cccatatatg ataaagattt aaccaatgtc caacctcgtg tgttgctgta
    17461 cgaccaaggt gatatggtac agccgtgccc ggtgcagccg gcccaccaac agaggaataa
    17521 aggcaaacta cgccatcaat atttgcagtg ccaccgggga aagttgcaaa gccaagaaga
    17581 ccactaccga gattacaaac ccaaaaattt agatatgaat ttctatccca tgcatcggca
    17641 ccaccggttg cagcaaattt catactagta ccagagggaa acgatgtaac ggttgtactt
    17701 acacgatgga taccggtagt ggcagcacct gatgggtcgc gctttgccaa acaaaattga
    17761 acattggttg ccgaagaaat gccttgaaat gctgaaggag tatttcctgc atcggcattt
    17821 gtacgcgaaa aatctttatt cattacattc aactgctcca gaatacgtgc atccgaaatg
    17881 ttttcggctg ttgtattata aagtacgtga aatacaaccg gtatggttgt aattgcgctt
    17941 gttttttcat ttgcatgtgt ttctacataa cgcgctgttt cgcgctcaat atcattcatg
    18001 cgttcacgaa gcgtaggatc tgctatcatt tgctcttgca aatgatccat tgtactgcaa
    18061 ccgcgatgcg attgttgggc ttgcgattga tttaacatca tagcacaagc cattcctgtt
    18121 agtaagagaa tttttctcat ttttttaaat aatttaattg gttttggaaa aatcaaatat
    18181 gctggttatt ttattaaaaa aatagtgtgt taataatttt ttataaaatc aacaaactgt
    18241 ttagcaagtt aatagaaaat ggtgacagga ataaacagtt ctaacagtaa gggtaataag
    18301 cagtgacgca atccccgaaa acagtttaaa atagtttaaa gcttgaaaac aaaaagtgca
    18361 cttcaaaact gcaactaaat tctaaaacta aaaactaagc agtgattgct tcgctgttcg
    18421 agaatactca ctgtgaaaat tcgcattgac gtaactaaga acaagtcacc acaaaaacga
    18481 agaaaacgaa tttcagagtc cacatttatt ttttattttt caaaggtatg agagactctg
    18541 aaatgaatgc taagtctaag cagagattgc ttcgcaattc gggaatactc actgtgacaa
    18601 ttcgcaaaga tgaactgaaa atagaaaact gaattcctaa actaaaaact aaaaacaatc
    18661 ctctctgcaa acgaaactaa gaatgccgac actacggtcg gcatcccgac caaagtgtcg
    18721 ggactaaaaa ctataaacta taaactttaa actaaaaact atattccctc tcccctattt
    18781 gctgccgcca catggcatag tacaatcctt tttcatcaag caactcactg tgtgctcctt
    18841 gctctattat ttttccacgc tccaatacat aaattgtatc ggcatgcatt atagtgctta
    18901 agcggtgagc tattaaaacg gtgatatgtt tttttccggt cgatacttcg cgtatggtgt
    18961 gggttatttc ttcttcggta atgctatcga gtgccgatgt agcttcatca aaaatgataa
    19021 gtgtaggttt acgtaacaat gcacgggcaa tggaaagacg ttgcttctcg ccacccgaaa
    19081 cttttacacc accttcacca atcatagtat ctaagccctt atcggcacgt ttgagtaaat
    19141 taccacatgc tgctttatta agtgcatcaa gcacttcggc atcggtggcg gttgggttta
    19201 caaacaacaa attttctttt atagtaccgg caaacaattg tgtatcttga gttacaaaac
    19261 caattttact tctcagttca gttagttcaa tttcatcgcc acgtttatta ttgtattgca
    19321 attcgccttc gcgtggttta tataatccca cgagcatttt tacaagagta gttttgcccg
    19381 aacctgatgg ccctacaaaa gcaatagtct ctcccaactt aatatcgaaa ctcacattgg
    19441 taagtgccgg tgttgaagca ctttgatgct tgaaggtaac attgcgaaac gatatcgatt
    19501 gcaaatcgcc aaggggtttt ggatttgccg gacgtgcttc aacaggtatg ctcattatat
    19561 ctttaaaatt atttaacgat gcttcggcct cacgataaat agcaatcaca tttcccaact
    19621 cctgcaatgg tccgaaaata aaaaacgaat aaaatgtcat cgtcatcatt tgacctatct
    19681 gtatctcacc tttaaaaatt aaatagtaca aaaggaaaac aaaacacgaa cgcaaaaaat
    19741 taaccgtagt gccttgtata aaattcaggc tgcgaataaa tcgcactttc ttcaattcga
    19801 gtttcaatat tttttgcgtt gtcgtgttca ggtgatttat ttcctgttgc tgcaaaccaa
    19861 ggctttttac cagttctata ttgcgcaacg actcggtagt tgaacctgcc agtgcagttg
    19921 tttcacccag aatttctttc gacactttct taattccttt actcaaaaaa ttacttgtta
    19981 tagcaagtat aggagctgtt aacaaaaaca caggcacaat catccagttc aaatttatgg
    20041 catacactac tacaaacaca agccctacca aagtttgaaa aacaatattt ataaacgaca
    20101 ctataaactt ctcgctgtcg gcacgcacct tttgtagctt gcccagagtt tcaccgcttc
    20161 gttggtcttc aaacacctcg taaggcaatt cgagcgaatg ggcaattcca tcactataca
    20221 actttgcacc catactttgt ataaccacat tagtataata atcctgaaaa ttttttgcga
    20281 tacgcgacac cattgccaca cctatcagcg ccaacacata tttaaaaata tcattaaacc
    20341 atgcaccctt attaccttta aaaatctcgg cagcatgcac accaaattta tcaatcatct
    20401 taccggcaat gtaaggatcc atcatcgaaa aacattggtt aatagccgcc agcaccatcg
    20461 ccagcagcag caaccattta taattcttca gataactcag caacaatttc ataactcaat
    20521 aatttatttg tgcgaaagta actttaaata attatccgca aaaaacaagc atgcgcatac
    20581 atttatagca acagtttttt acgccacaaa taaatatttt ttattgcggg ggctggttag
    20641 ccctccggtg cattgcgcct tgcgctatgc aataaatacg cttcgcatct catttctaat
    20701 tcaacctaaa actgatttat tgttgattca aaaaacattt atctttgtta catgacagac
    20761 aacaaagaaa taattctcaa cagtgccact ttcaacacga aagtaaaaac atatatattt
    20821 tttgtagtac tattttacct tttcataagt gtaataggat ggatagttat accattctgg
    20881 gtattaggtt ttgggcaatg gcttagcaac aaattttttc atactttgaa gtgcgagcta
    20941 tccaataaga atttaaaatt ttcgaaagga ctcattatac atatcgaaaa aaccattcca
    21001 ctcgaaaaca ttcaggacct ctctttcatc ggtggccctg tattacgttg gtttggactt
    21061 acacttatta aagtagaaac tgcaggcggt ggtggtggcc atcatgacaa taacatcatg
    21121 agtatgatag gcataatcga ttccgaaaat ttcaaaaccg aaatcttaaa ccaacgcgaa
    21181 aaaataataa aagaaaaata cacttttgca gcacaaccca acacaagtat ttctgacaca
    21241 caactgctta ccgatattaa aaacgaattg attgaaataa agatggcgtt gaaaaagtaa
    21301 ttcgcttttc cacagcataa cagttgtgct taccatcaac aaggaaaaat tttcaggtgt
    21361 cgtaaaaaac taatcgtgtc gcacctgctc catcacttta aaaatgtgca gcaagtatgg
    21421 aaataatgcg tccatacttt cttgtgctcc tcgtgtgctg ccgggcaatg caagcacaag
    21481 ggtattgcct ataagtcctg caacactgcg cgatagcata gcatatggca tatgttgcat
    21541 tccgtaactc cgtgcggctt cagcaatgcc gggaatattt ctatcgaaca aaggaagtag
    21601 tgcttcggga gtaacatcgc gaggcgaaag accggtgcct cctgtaaaaa ttattaaatc
    21661 atatttatca gcacacaacg atttcgtttt cgcttgtatc aattccattt catcggcaat
    21721 gatggaatac tcgtcaatgg caacagtata tttttctaac ttagttataa tggctttgcc
    21781 tgcaaaatcc tgtttcgtgc cggccgaaat actatcgctg caaaccacca cagcagtttt
    21841 tattgtacga tttaaaatat cattgtattg cgatttgcca cctttctttt tcaataattt
    21901 tatagaacta atttctattt ccttatcaat tggttttagc atatcgtata tagtaagggc
    21961 tactaccgat gctgcatgca tagcctctac ttctactccg gttttataaa tggtacgaat
    22021 ttctacttca atgtttatct gcaaaccttc tatagtatgc ctcacctctg caaactcaat
    22081 tggcaatgga tggcaatcgg gaatcaagtc ggcagttttt tttgcagcaa acaaacccgc
    22141 aatacgactg gcttctatta catcaccttt aggtacggtt ttacttttta tcgcatcaat
    22201 ggttgcctgc aaactcactt ttacaatagc ctgtgcaata gcagttcggt gtgtatttat
    22261 tttgttggtg atgtcaacca tttacttttc tgttagccga aaattatttt gtatcaatgc
    22321 aattaaaaaa aaatgttgta tgacaacaag cgataaattt ggatgctgaa aattttattt
    22381 ttcgtggatg gtttatattt tcgatttcgg taaaacctat taaagagaga cgcgctgtag
    22441 cgcgtctcta cggctatacg actgctgcgg ctttatattt tcctttcgat tttcgaatgt
    22501 ttactgcaac tactttatac tcgggacaca tggcttcgct gtcgctgatg ctcgatgtga
    22561 ggtcgttcat ttttacttcc gggaaatgaa atgtgctcga caatattccc ggttttacct
    22621 catcggtgat gaaggctttt acatcgattt ttcctcgtgg gcttgtgatg caaaccatat
    22681 cgccatcttc tatcaaatgc ttaacagcat cctcaggatt tatcatcaac acatcgtctt
    22741 tcaatattac cacattgcct gttctgcgtg tcatagtgcc gcaattgtaa tgttcaagtt
    22801 cacggtttgt tgtaagaatg tatggaaaat ctttttcgtt ctgtactatt tcttccgatt
    22861 gtttccatgt tgcagtttga aatttaccta atccgcgttt gaatgtttcg gtatgcaaaa
    22921 tttcggtatc gctgccatct ggcaacacgg gccattgctt tccattctca ccaagttcat
    22981 cccattttac tccggcaaaa aatggtacta tctgcgatat ttcttcgagc atcgttttag
    23041 gatcataatc tgcctgcgca tatcccatgc gattcataat gtcaactaca atttgtccat
    23101 cgggctttgt accttctatt ggcggtacaa cggcttgtac tttttgtatc ctgcgttcgc
    23161 cattggtaaa cgtgccgctt ttttcgagaa acgatgatgc cggtaaaacc acatcggcca
    23221 tttttgctgt ttcggtcata aatatttctt gcactataag taaatctagt ttactcaaag
    23281 cattttttac atgcgttgta tttggatctg tttgaacatt atcctcaccc atcagccaca
    23341 tggcttttaa ttttccgcca atggcagcat catacatctg tggaattttc caacctacgt
    23401 gaatcggcaa ttttgcattg taaaaatttt catacatgtg atgatattcc acattggtaa
    23461 catcgtaata ccccgcaccc tgatgtggtt ggcagcccat atcagctgcg ccctgcacgt
    23521 tgttttgccc gcgcaatgga ttcactccta ctcctcgtct tcctatgttg cctgttatca
    23581 tggcaaggtc ggcaataagc ataaccgtat acgtaccctg cgaatgttcg gtaacaccaa
    23641 ggccatgaaa cgacattgca ttgggcgcat ttgcataagc cagtgctgcg gccttggcaa
    23701 gatttttatc cacacctgtt atgcgttcga gttcatccac attcaaactc aaaatatgct
    23761 ttttaaaatc ttcgtaacct tcggtgcggg catcaataaa tttttgatcg gcaatacctt
    23821 cttgaacaat aaagtataaa agcatattca acatggccac atttgtaccc gggagaagtt
    23881 gcaaatgata cgttgcatag cgtgctattt cagttctgcg tgggtctatt actatgctgg
    23941 tttttttcat ggcaaactgc ttcaactttg cccctgtaac aggatgggca tcggtggggt
    24001 tggcacctat taccattatg caatcggtat atttcaaatc gaccacagag ttggttgcag
    24061 ctccggtgcc aaatgtacgt tgcaatccaa gtgcagtagg tgaatgacaa acacgagcac
    24121 aactatcaat attattggtt ccaattacag cgcggaaaaa tttctgcatc aaataatttt
    24181 cttcgttggt acatcgtgcc gaagaaattc ctgccaatgc atcggggcca tattcatttt
    24241 taatggaggt aaatttttgc gtaataaaat cataggcttc gtcccaactc acgggtacaa
    24301 attcaccatt gcgttttatt aacggtgacc gtaaacgttc gggatgattg taaaaagaaa
    24361 aagcaaaacg gcctttaagg caagtgtgcc cttcatttac ttcggcatcg tatggtgcct
    24421 gtatcgattt tactttatcg tttacaactg ctacttcgag gttgcatcct acaccacaat
    24481 aagtgcaaat ggtccgcact ttcttatcgg caactaccga tttgctttca aaaacatccg
    24541 atattgccga tgtaggacaa gcctgtgcac atgctccaca actaacacaa tccgattcga
    24601 aaaacgattt attatcactc tttacaatat gactatcgaa gcctcggccg gccatagtta
    24661 acacaaattc gccctgcact tcgtcacatg cacgcacaca acggaaacaa tttatacact
    24721 tcgaaaaatc ggaggtcatg tatggatgac ttaagtcttt ctttttgttg agatgatttt
    24781 ttccttcggg atatcgcaca tcacgaatgc caacacgtgc tgccacagtt tgcaattcgc
    24841 aattattatt tacttcacat gtgaggcaat ccaaaggatg atcggtaaga acaagttcga
    24901 taatattttt gcgcagtttt gtaattcggg cactgtcggt atagatgtaa ctattttcaa
    24961 ttacaggtgt atggcacgat gccatggctt tggcattgcc gttttgcacc aaggccacat
    25021 caacactgca aaccctgcat gagccaaatg gttcgaggtt aggcgcatcg cacagtgtag
    25081 gaacaaaatc tttaccgaag tatctacgta taaattttaa aattgtatca ccctgagcaa
    25141 tagcatattt tttatcgttt ataaaagcaa atttttccat cgtaatttat tttttaaaat
    25201 aaggcgaaag ttctgtatta aaatatttca tagcattttt aattggcaaa ggcaacccac
    25261 cgcccaatgc gcacagcgag ccggtttcga gtgtttcgag caaatcggtc atcaactcgt
    25321 aagggatttt ataatcgctt tcgagggctt tgtccaccat ttcgtaacca cgtgtgctac
    25381 cgagcctgca tggaaaacat ttgccacaac tttcgtgtgc tgtaaaccga aacaaatggc
    25441 caatgtaatt tataagagga tatttttcgg gaatacaaac tactgatgca tggccaagta
    25501 aaaacccttc gcgggcaaat gaatcaaaat cgactgtaag tttttcaatc atactaacgg
    25561 gcaccaatcc accaagcggg cctccaatgt gcattgcttt aataggttca cgaaagccac
    25621 cgccaagtgc gtatatagcc tccgttaatg gcgtgcccat atctacttca tatattccgg
    25681 gtttattaaa aaatccatcg agcgatatta attttgtacc ggtcgacttt gccgtgccta
    25741 ttgctgcgaa tgctttacca ctatgttgca ttatgtaagg cacattggca agcgtttcaa
    25801 cattattcac cacagttggt ttgttgaata gcccttgctg tgtaggataa ggaggccgca
    25861 cacgcacttc ggggcgttgt ccttcgatgg atgaaagtaa ggcggtttct tctccgcaaa
    25921 tatatgcccc ttgagcacga atcactttaa agttgaatgt gaagccactg ccgttaatat
    25981 tttcgcccag caatttcatt tcatataatt gctttatgca ctcatcaata atagtgatag
    26041 attcgggata ttcgccacga atataaacaa caccatgttg agcaccggcc atgtaacctg
    26101 ctatcatcat tcccataagc aacgaatgag gacgttgctc cataatgtaa cggtcgctgt
    26161 atgcgcccgg gtcgccttca tcggcattgc acacaataaa ttttgtatca ccacttgccc
    26221 ctctgcacga ttcaagtttg aaagccatcg aaaatcccgc tcctccgcgt ccacgcaacc
    26281 cgctatcttt caattcgctg agtaattgtt cgggagtctt tttcaaacat tccagaaata
    26341 tgttgtaata agattttaca tcgccataat cggaagtaag aactgccgtg ccacgacttt
    26401 caacattgta attatctata ggtgaggttg cccctttctt tatttcttca atttgcaaaa
    26461 catcatcacc cgaataattt ttgccggcat aatgaaatga attgttttca tgacaacgac
    26521 caaggcaaca catttctcca atttcgtttg cctcaaaatg tggtgccaat tttttatgca
    26581 attcatcttg cgtgccggca gtcatgcatg aacttccatt gcatacataa actttctttc
    26641 ctttattctc gggtttcaaa aaatcgtaaa acgatactgc accatatacg ttggcttttc
    26701 caaccagaaa ttcgtcacac agtttttcca ttctagcggc cgagggtgtg ccggtttctg
    26761 cagctgcaat tccaagctct tcgaaaagat ttttcgtcaa gccctttcta cctgataatt
    26821 cactaagatt tttcgacata attattttgg tttacacaaa tgaacaaaat atttttcatt
    26881 aggtttatgt tttcaaagat gggaatttat gtagaactct acaagtggtt ttgaaaatat
    26941 ggttgaggag ttggaatggc tattttttaa tcgcaatacc cccaacacat tatattgact
    27001 cataatactt aggagctata ctaaccgaaa aggaactttt gttttaatct aataaacatt
    27061 tatcgcaatg cactttactt aattaatcca tttataaaaa cctctttgga attctttacc
    27121 ccttccacat tggaataaac agttaatttt ttgccgcggc aaaatgccat gagggtgatg
    27181 ccggcttttt gtgcttcgtc aactgcgaga gatgatggtg ctgatacaga agcaagaaaa
    27241 ggaataccgg cactttttgc tttgctaaca atttcgtacg aaatgcgacc gcttactgta
    27301 aggcatttta ttttgtctaa tagattattg ttgagcaaat aacctatcac tttatctact
    27361 gcattgtgtc ggccaatatc ttcgtgcata gttaaatatt gtgcgtcaat agtgaaagca
    27421 cctgcggcat gcgtaccacc cgactgctga aaagattttt gtttttcgct cacttgatca
    27481 aacatatttg caacaagtga tacatcgaga agttgttggt tatttatttt ttcggaatct
    27541 aaatcttccc actccgattt accgcacatg ccacacgatg acgatgaaat tacattgcgc
    27601 gtaccggcaa aatctttcat cacaagttgt ggtggtacta ctacatttac atgcgtaata
    27661 aatcccgcgc tgttggtttc gcggatttca actttaggtg taaacgtttt atcgacaatt
    27721 attttttcgg aaaataaaat gccgcgggca aggtcaattt cgttacccgg tgtttgcata
    27781 gtgatagtaa aaggaacatc attcacagca atacttaatg ccacttcgat agcaagcgtg
    27841 tcatgcactt gcgaaaattt ttcgccatcg aaaaacaaac cattgtatag tgtggtattc
    27901 attttgggtt taacttaatc ccgatatttt tccgttgtta tcaatatcca tgtgctctga
    27961 tgccggcacc acaggtaagc ccggcatacg catcatatcg ccaagaatag gaattataaa
    28021 acctgcaccg gccgcaaatt caaactcgcg aacgtttact gtaaaacctt ttgggcggcc
    28081 tacaagtgtc tctttatccg ataaagattt ttgagttttc gccatgcata tcggcaattt
    28141 atcgtaaccc aagtcattta tcatttttaa ttgagtgcga gccttcgaag aatattctac
    28201 accatcggca ccataaattt ctgttgcaat gcgatttatt ttatcctcca ctttcatgtt
    28261 ccaatcgtaa agaggtttaa atttattatt gccattttca atcacatcaa gtactgcttc
    28321 ggcaagtttt gtagtgccat cgccaccttg tgcaaagcca taagatacaa cagctttggc
    28381 ccccattttt tcgcattgcg tttgcacata ttcaatttct tcggtggagt cgtttggaaa
    28441 ataattgatg gccacaacag gagtgatgcc aaattttttg caattttcta tatgcttttc
    28501 gaggttcaaa aatcctttaa ttactctatc gatactcggt gaattatatt cttctttttt
    28561 tgctccacca tgatgacgca atgcgcgaat agtagccaca agcacaatag catttggtgc
    28621 aaggccggca taaccgcatt ttatatccat aaatttttcg gcaccaaggt cggcaccaaa
    28681 acctgcttcg gtaacaacat aatcgcctag tgacaatccc atttttgtag caataatggt
    28741 gttggtaccc tgagcaatat ttgcaaatgg accaccatgt aatatggcag gattgccttc
    28801 gagtgtttgc acaagatttg gctttatagc atcctttaat aaaatcgcca ttgcgccctg
    28861 agcctttaaa tcacgtgcat atattggttt tttatcgaat gtaaatccta caaaaatatt
    28921 tgccaggcgg catttcaaat catcaatatc tttcgacatg caaagtatgg ccattacttc
    28981 cgaagccgga gtaatattaa aaccatcttc gcgcggaatg ccatttaagg tgccgcccaa
    29041 tccgatggta atttgccgaa gtgctcggtc gttcatgtcc attacacgct tccactttat
    29101 ggtgcgcggg tcgatgttga ggttgttttg tttgttttgc aaattattat cgatgagtgc
    29161 ggccagcaaa ttatttgcct tttctatagc agcaaaatca ccggtaaaat gcagattgat
    29221 atcttccatt ggaattactt gcgagtatcc gcccccggcc gcaccaccct tcataccaaa
    29281 aacgggaccg agagatggct cacgcaaaac aacaatagat tttttgccaa gtttgtttaa
    29341 gccttcgttg aggccaatgg atacagtggt tttgccttca ccggcaggtg taggattaat
    29401 ggccgatacc aatatcaatt tgctctttgc aatttttgca tcgtctatta aatttaatgg
    29461 cagcttggct ttatgtttgc cataatattc taaatcatcg gccgtgattg atatttttgc
    29521 cgccacatcg cgtatgtgta aaatgcttgc ctgttgtgca atttcgaggt cactcatcat
    29581 tggtttagta ctcataattt tttttataat gtgttattat tttttttcca aacggaactt
    29641 tcgtcttcaa atatttcttt accccacacg ggagcttcac tttttattcg ctcaacaata
    29701 taagcgcagc tttcgatagc agcatcgcga tgtgtcgatg aagtaaaaac aaataaactt
    29761 atttcccctg tcatcacttt tcctaaactg tggtaaatat gcatgcaagt tagagaatat
    29821 ttttcaaatg catcttctct tattttatga aaaatttctt cagccatttc gctgtaggct
    29881 gtataatcga ttgctgctac tttcttatgg tcaataatat cgttgcgcac ctgtcccaaa
    29941 aaaatactgt gagcgccaat ttctgttttg gcgctgtgct tagcaatgga gtcgccaata
    30001 aattgcggac tgattgcccc ttctttaaat acttgctttg gtttgtggtt cataaaatta
    30061 agcttgttgt tcttcaagta ttaaccaggc cataatgcca ccttccaaat ttataaatat
    30121 ggcttcagga aaatgcttgc tcaatatatt ggcagcttgt tgcccgcgcc tgccatgatt
    30181 gcaaaacgtg ataaccgtgc ctgtatacga caagcttgaa atatctttac taattgtact
    30241 caaaggaata tgtgtatgaa tgaaattgtt tatcgttggt tcttcattca attcgcgcac
    30301 atcaataatt tcgaggtgtg aattttgcaa atattcagaa aactgcttcg gagatatttt
    30361 tttggcgtca ctcatataag tacaaaataa tttatgacta aaatgtttaa actgttcaat
    30421 tgtttgaggt gcataaatat gtgcatttgg atttggctca atactaaaaa cttgttgctg
    30481 atgattgagg atgtcataag taagaagttt attcactaac aaatcgccac tgcctataat
    30541 aaatttaatg gtttcggtgg cttgtaacag gcctataatt cccggcagca cgcccatcac
    30601 tccggcttcg ttacaggata gtactgcatt ttcatcggga ggttgaggaa acaaatcacg
    30661 gtaatttgtc gaataatttt gttcagtcat tcgtacatta aaaacagcaa cttgtccttg
    30721 ccatttcaaa actgcaccat gtaccaaagg tttcttcaat agtacacagg catcgttaat
    30781 caggtaacgt gtttcaaaat tatcggtgcc atcaatcact atatcataat cggaaattaa
    30841 atcgatggca tttgtattgt taagttttag ttgatgtgct attacattta tttcgggatt
    30901 gagttgctgt aattttgcct ttgctatttg ggctttgggt tttcctatat catttgtcga
    30961 aaataaaatt tgacgatgga ggtttgataa ttctatatta tcgaaatcgc aaattccaat
    31021 tgtacctaca cctgccgctg ccaaatattg caatgcaggg cagcccaatc ctccggaacc
    31081 gataaccaaa acttttgagg ataaaagttt ttcctggcca tgtgtaccaa catgaggcaa
    31141 tagtatttgc cgactataac gttcactgtt tatcattacc ctcccgaaaa tggcggcagc
    31201 aaagcaactt catcgccatt attaaaattt acatttccca aaactatttt cttgttgatg
    31261 gcaatagcat aacttaatga ttttaaagtt ggataatttt tttcaaaata cgcacgcaac
    31321 tcatcggtgc ttattaggtt atccaactcc atttggcccg atgacagcac atcggcaaca
    31381 gcaccaaatg caagaatttt tatttccatt gcacaaacat aattaatgta acggtgaaag
    31441 ataaaaatat ttttaccaaa taaatgaaat gggctctgct aaacatgtaa taaaaaaata
    31501 tttcccgcag ataccacaga ttatttttac aaacttctgc tctcatcagc gaggaaactt
    31561 atcttccaaa gtcagcggaa aaaacatcgg cacaggcttg tttaaattgc tattggtata
    31621 aagtgattac gaaaaataat ttttcgcatc cttcaattct tcgggagtat taatattttt
    31681 aaaaaaattt gcgtcaaaca aaatattata gtctaacact aattgcttta gcaaaaacag
    31741 tttcaccatt gattgcagtt ttcgctcatc ttttaaaata aggatttgcc acttatccaa
    31801 aatatttttg ttatacactc cacacattgg ttgcaagtga ttttcgaaaa caggaatagt
    31861 tattccgtgg tcgagtgaat gcatcattat atattcaatt cctttggtgc gaataaaagg
    31921 catatcgcaa ctcacaataa gattgtgttg agtgtttgta tgctttaatg cagaaaatat
    31981 tccacctgcc gggccaatat tttttatctc atcttcaata cattcaaaac caagcttttt
    32041 atattctgaa ttattcgtta caataaaaat atttttacaa agcggtttca gttgcgctat
    32101 aacataatct atcattggct tttcgttcaa caaaattaat cctttgtcgg cacccatgcg
    32161 cgaacttttg cctccggcaa gtatgtaggc gttcattata ttttttacaa ctggcactat
    32221 catgttacaa ccaattttat gcaagccact actaatacta ttgctaaaat gcgttgcaat
    32281 gtttttgaat ttaatttttg tgcaccatag aacgaaccta taaaaccacc tgccaatgcc
    32341 accaccaaca taaccaccat ttgattagag aaaacaaaat tctgcgaaga gatggcagca
    32401 aggccggcaa tagaatttac gaaaatgaaa agtgccgaaa cgcaagcggt agtttttata
    32461 tcggcccagc gcaacaataa aatcaaaggg ctaagaataa tgccaccgcc aatgccaatc
    32521 atgcctgata aaaaaccgat tgcagcacca attattaatg acaaaataat gctgcttttt
    32581 tcggtttcgt ttttcgattt ttcgaattgc aaacccagta attttagtat cggaaaaatc
    32641 aaaagcacag ccaatattcg tttgtacata acgggatcga gatgttccaa gccaccaaga
    32701 aaagccaatg gaaccgaggc aatggcaaaa ggccaaaaca attgaaatcg aaaatatcct
    32761 tgtctgaaat acggcacgaa ggcaacaaga cttacaaaaa tattcaacac cagtgctatg
    32821 gagcgcatag tttcgggcgc aaacgaaaac agtgccatca acattaaata cccactggca
    32881 ccaccatgcc ctaccgaggc atacaacatt gcgactgtaa ataataaaat gtaaaataaa
    32941 atatcttgca caatcatagt tgtaaaacga aaactataaa atcattctta ttgaaactaa
    33001 atctcccgca ataaaaattt ctttttctgc atcaaattct acaatacaat tggccatggc
    33061 aaacgaattc atcaaatagc tttcctgatt ttttaaaacc gtaacagcat tttcattttt
    33121 attgcctttc ataaagtgtg taagctgtgt tttcttttcg taattatttg caagtggcaa
    33181 cgtgtatttt gcgaaataat ttcgttgtgt aaaatcggca attacgtcaa caatgtaatg
    33241 ataaaaacag gtaagcacag cggcagggtt tccgggcaaa ccaaatacaa gtgtatcgtt
    33301 cttacgcccg aaataaaagg gtttaccggg tttctgtttt actttgtgaa atattttttc
    33361 gacaccggca ccggttaatg cgtccgccac aaaatcgtaa tcgccaactg atacaccacc
    33421 cgttaaaatt ataatatcgc tttgcaaagc ttgctttaca gcattggtaa tttgcagttc
    33481 atcgtcagtt attatttgaa cagataatgg ttcaatattc aattgattta atgctgcaat
    33541 taatccaaaa gaatttgatt cataaatttt ccctccatca atactttgcc ccggctgcac
    33601 caattctttg ccggtaatta tgacactcac ttttggctta tcgtaaattt caacttcggc
    33661 aatgcccatc gaagcaatat aagaagtggc aggaggcata agcaaaattc ctgacgacaa
    33721 aaccacttcg ccttgtttgg tttgcgaacc ttttaatcgc acatttagtc caacagatat
    33781 tttatcatca attatttcaa tcgtgctatt ttgcgctata actttttcct gcatcaccac
    33841 agtatcggca ccctcgggaa ttggcgaacc agtataaaca cgtgcagcct caccaggcaa
    33901 taattttatt ttcgaaatat cgcccgctgc aattttttgc gttaccgaaa atgtttttga
    33961 gtgctgtaaa tctataaaag caaaagcata accgtccatg gctgattgat tgaaaggcgg
    34021 tgtatggata ggtgcaacaa tagtttgtgc caatacacat tggttagctt gtaacaatgg
    34081 caccctggca atgcgcttgg ctaaaaaatt atcagctata atttgtttgg cttcagtggc
    34141 agatatcatg atgctaataa acggcaattt atttactaaa cgaaaatcat ttgtcacaac
    34201 atttgtcttc agttgataaa gttattggca tttttccaat aagtattcaa gcgctttatt
    34261 agaggcacgg cacaacttta ccctacatag tttaagttgc tttatcaata aaaacaaatc
    34321 acaataggcc aattagaaag tttttacctt tattacctga caaagtgata caaaaaaaaa
    34381 ttcgcttacc aattgaactt aaaaaataaa aaaaagcacg acccaaccaa agtatacaaa
    34441 taagcatctt ataataaaat caatagttac attttttaaa ctaaaacaaa acaaatatga
    34501 acatcaaatc aattgcattg gcaacaatgc tcgcaacagg aattgcaact gtaaatgctc
    34561 aggaattaaa aggcaaaaaa tacgttggtg gcaattttag ccttggcgta aacagcgaaa
    34621 acgaaaagga tgataaattt tcgaaggata atctcaattt tgaaatgaat gtatcgggcg
    34681 gttatttttt cactaataaa atggcattgg gatttttttt aaagactgct ttttcaaatt
    34741 atacaaacag cagcaaaact tctttttcgg atattgatga caaaaatata tcaaaccaat
    34801 acgctggagg cgttgaacta acaaaattta taactataaa aaataaattt tcgtttgctt
    34861 ttaaaaacca attagtattt agtataatga atagtgatta taattacaca tctgataata
    34921 taaatgggaa ttttaattct attaccaaat caacaacaag aacatttggc tataatttta
    34981 ttccagaact ccattatttt gtaagcgaaa aattttcttt cagcgcacaa atgggaagtg
    35041 ccggaattaa ttatggcaac accgaggagg atgattttaa taaacaaact tttaatggtg
    35101 ttatgcttga aaaccaatcg gtaacaacca aatcgacaga atttagtggt gatgttcgct
    35161 tttcgttgag gcaatttagt ctaggcctgg tttactattt ttagaaaaac gtaaacaaaa
    35221 caacagagaa ccggcatcta taaagttgcc ggttttttta tttataatat tggctttgtg
    35281 tttcttttgt aaagaaatat aaaaggcact atttatgctg cacatatttt aattatcttc
    35341 gttgtgaaat taaatatgtg tgatatgaaa aacttgttac gttatattat tttgggtttt
    35401 atattaatac ttggcgtgaa tgcctgtaaa gacaaagacg accccgaaat accatacctt
    35461 ggtttgaatt actatcccga tgaaattggc cactgggtaa tttatgatgt tgattcaact
    35521 tacaaatatg gtttcgattt agttactcat catgcgcatt atcaaataaa agaagttatt
    35581 accgacacat ttttggataa cgaagggcgc aaaactttac gcctggagcg ctaccgtaaa
    35641 gacagtatga gttaccccga ctggacaatt tatcaagtat ggacagcaaa taaaacagca
    35701 tcgaacctgg agcgttacga agacaattac cgctatgtga aattgcagtt tccgttgacg
    35761 cgcaatgcca catggaatgg aaacagcaaa aacaccctca atgcttttga ctatattatg
    35821 acagatgtac acgaaaccga aacggtagga aaactgttat ttgattcggt gagtactgtg
    35881 ttgcacaccg atgatacatt aaatttaata gagcgcactt atgaatacga aaaatatgct
    35941 gccaatgccg gcctcgtata ccgatacagt taccgcctgg aacaacagcc ggtgcagggt
    36001 cagctcaatg tatatgacac taccctattt acaatttata aagaaacagt tttagattat
    36061 aaaaaataaa atatcatcat gattaagcat ttaaacaaat tcgtatttat ttttctttgc
    36121 atttcgggaa ttttttgttc caataatttg atggcacaag acaggtacgt gctgcaactt
    36181 gccgacaaaa ataattcgcc atttaatatc aataaccctt cggcattttt aagcccgcgt
    36241 tctattgccc gcaggcaaaa gcaaaatatt gccatcacca caaacgactt gcctgttaat
    36301 ccttcgtatt taacagcaat atcaaacaca ggcgctacta ttttaaatta ttcgaaatgg
    36361 ttgaacactg ttaccattca aactgccgac tccaatatat tgaacgcttg cattgcattg
    36421 ccttttgtat tgtcgggcaa taatgtggga cgtgtagcac aacccaacga aatatcggaa
    36481 gtgagcaaaa caaaattcag cgaaaagtac aatcgcaata ttccatttgc cgaacaatca
    36541 aacagcacaa gtaaagttgc ggcttttaat tatggcccgg gctttcatca aataaatatg
    36601 atgaatggcg atgccttgca caactcgggt tatagtggcg atggcatgat gatagccatt
    36661 atggatgccg gattttggcg tgccgatagt tgttccattt tcgatagctt atttatgaat
    36721 aatcaaattg tcaccacatg ggattttgta cttggtaatt cgtatgtgtt cgattatcat
    36781 agtcatggtt catcgtgcct gtcgacaatg gctgccaact tgcccggagt aatggttgga
    36841 agtgcaccac atgcaaaata tattttgttg cgcaccgaag atggcgctac cgaaaatatt
    36901 attgaagaat ataactgggc cgctgcagca gaatttgccg acagtatagg tgccgatgta
    36961 tttaccactt cgcttggata tacccttttt gataacccgg cacacaatca cacttatgca
    37021 acgctcgatg gcaacacggc cccaatgact attgccgaag atttagcagc agaaaaaggc
    37081 attgtggtaa taaacagtgc aggcaacgaa ggcaacagcg cctggaatta cgtcagtgtt
    37141 cctgccgatg ccgatagcaa tctggcaatt ggcgctgttg acagtatgga acaatatgca
    37201 agttttagcg gtaaaggccc cactgttgat gggcgtgtaa aacccgatgt gtgtgcacaa
    37261 ggtgccggta cttatgtagt gtggcctggc gatggcagca tggttcccgg taatggcact
    37321 tcattttcgg gaccactatt ggcggggctt gcggcttgcc tctggcaaac tcaccctatg
    37381 cttagcaata tggatattgt gcaggcgata aagaaaagtg cctcgcaata tgctgctcct
    37441 gatagtttat tgggctatgg cattcccgat tttacacagg caagtatgat tcttggcggc
    37501 atcgaccctg catcattacc caccgaggac gaaatttatg ctctgtatcc aaatccatcg
    37561 agcaacgatg ttacggtgat gttttttact accacagcac aaacaataac tgtaagtgta
    37621 tacgatgcca ttggccgaaa agtgtttgca aataaaatgg atgtggctcc aatatttatt
    37681 aatgaaattc cgataaaaat aaataacacc tcaggagtat ataatctgga aataataaac
    37741 gaggctggca caaaacttca caagtcaatc gtgcgtcaat agacttactt tatagtttcg
    37801 ccaccacaac tgctgccggc acctgccgca caaccgtagc aatgcttatt caaaactata
    37861 tttcggtttt gtaattcgtg cgcatcaaaa ttatcgatgt gttgaatttt attttctact
    37921 tttaagccaa gcatttgatt aaaatcgcaa tcgtaaaggt agccatccca tccaacagat
    37981 aaagtattgc gacacataag acctgctacc gctgcgggat tgaaggcatt caccagtcgg
    38041 ctcatataat tttcgtaatt attgctttgt atcaaataat ctaaaaagcg actgatgggc
    38101 atattagtta tacaaaacaa attattgaag tctatatcga aatcgcgctt gagtgtaagt
    38161 ttaaaatcgc gctcgagagt agtttgtgcc gctggcaaaa atgcccccga aggattgtaa
    38221 actaaattta atatcagtcc agtttcggct ttgccatatc ctacactgtt tagnnnnnnn
    38281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aaacacgcca tcgccacgtt
    38341 gcttatcggt acggcttgca ttgtaaaatg gcaacgatga aattacttcc accttatgct
    38401 ttgcaaaaaa tgacggtaac gttttatatt tttcattagc cacaataatt gtgagattgc
    38461 atcgcactat tacatgcaca ttaagttttg tgcattcctc aacaagccat ataaaatgag
    38521 gattcatttc cggagcacca cctgttatat ctactgcgcg aatgttgttt gcagcaataa
    38581 tatccaaaca cttctgcata gtttcgcgtg tcattatttc ggtacggtcg ggtccggcat
    38641 ccacatggca atgtgcacac gtttggttgc acatataccc aacatttatt tgaaaaatat
    38701 cgatggcttg cgcacgtaga ggaaataaat ctgcttgctt caacttttct tcgaaaggta
    38761 aaatttcggg caggttgttc aactgcgcaa tttgattggc tgcagttgcc aaatcattat
    38821 gccttgcatg tagcgatttt atcatctctg ttttattgac tttgcaccat gtgtgtgtaa
    38881 attaaacgat acgcaattta catacttatt tctttcacct tattcatcat ttgcacgcca
    38941 tgtacaagtg ttgcacctcc gcgtatagca gctgccacat gcacggcttc catcatcatg
    39001 gtttcgctgt tgcctttttc caccgaatcg gtagtgtatg catctataca atacgggcat
    39061 tgcacagcgt gtgctacggc aagggctatc aacgattttt cgcgggccga taatgcacca
    39121 tctttaaaca catcgttgta atacgaaaaa aatttgtcgg caagttcttt ttgaaactcg
    39181 cctatgttgc caaactttgc caggtcggct gcattgtagt agtgatttga tgtcatttgc
    39241 ttttatttta tttctaaata ttttgtgcgg taattaattt gtatcctctg caaatatgct
    39301 ttaaataatt tgcattcgat tgtcgtgata acgactattg agcatcataa aataattttt
    39361 gattcattgc gtaacaaagt cgtttgttat ttgtctatcc ttagtaaaaa gttaacaagc
    39421 attttaaaat taaaaagtac atttgccgaa ccaaaaatca aacttaaaat atgaaattaa
    39481 aaacattaac cattgcactt gttgcaatat tatttaccgg ccttacaagt tatggccaaa
    39541 cagtgaacga agtagtagac aattacttta aagccattgg tggtcgcgat aaagtaaaga
    39601 gtataaaaac catgaaaatt accggcaagt tttttatggg gccaatggag attcccgttg
    39661 aatcatcggt aaaaaaaccc aataaagttt acagcactgc caccgcacaa ggcatgacac
    39721 aacgtatggg cttcgatggt aaagatgctt ggcagtttag tccttttatg ggcgatacag
    39781 tgccacacaa aatgcctgaa gaaaattcac gcgatatgaa agaccaggcc gatatagaag
    39841 ggccattgat ggactataaa gaaaaaggca gtacagttga gttgataggt aaagaagata
    39901 tagaaggcac accggcatat aaaataaaga tactcaaaaa atcgggtgac ctcgaatatt
    39961 actttataga tgctgaatca tttttgttgg taaagtctgt ttcgaaaaaa agctttcaag
    40021 gcaaagaagt agaatcggaa acagtgtata gcgactaccg cgatgtggat ggaatgaaaa
    40081 tggcattcag caacgaatcg cgacaagtgg gtgaagaagc aggccagaaa tttattatcg
    40141 ataaagttga agtgaatgtt gatatgaatg atgaaatgtt tttgctaccg acaaagtagt
    40201 aacaatcaaa aataaaactt ttgaaacgcc agtgaaaaaa atcactggcg ttttttttaa
    40261 cattactttg attaccgctg taacaaatct gttggcaagg ccatctatat ttgcagcaat
    40321 taaatttcct gctccaaaaa aataaaatac tcaatcgtta tgaaaaaaat attggcacta
    40381 ctcattgtaa ccgtatgtgt atacgatgcg ttttcacaaa caaaattcac caactccatg
    40441 tttggccctt atgaggcgcg ggcaataggc cctgcggtga tgggtggacg cattactgct
    40501 atagaaggac ttccaagcga tgcccgcaca atatatgtag gcactgctgc aggcggtatt
    40561 tggaaatcga caacgggtgg cactattttc aaaccgattt tcgataagta cacacaatcg
    40621 attggtgcta tggccataga cccacaaaac gaaaaaacga tttgggcggg tactggcgaa
    40681 agtaatatgc gtaacagtgt gagttatggt gccggcattt ataaaacaac cgatgcaggc
    40741 gacaactggc aactcatggg cctcgatagc accgagcata taagcaagat tgccattgat
    40801 gcaaaaaatt cgaacactgt atacgtagct tgccccggcc cgctatggag cgacagtcct
    40861 caccggggat tatacaaaac aaccgatggt ggcaaaacat ggaataaaat tttatttggt
    40921 gataataaaa ccggctgtgc cgatgtgatg gtcgatccta aaaatccaca aatagtatat
    40981 gcctcgctgt ggactttcag gcgcacgggc tggtcgtttg agagtggagg cgataagagt
    41041 ggattattta aaagtaatga tggcggttct acatggcgca aaattcaaaa agggtttgat
    41101 ggacaaatgc ttggacgtat atgcttagcg ttagcaccat cggcacccaa taatttatat
    41161 gccattgccg aagcaaaaga aactgctttg tatagttcga aagatggtgg cgaaaattgg
    41221 atcaagcaaa gcaacaatca aaatgtaagc tggcggccgt tttatttttc ggcaataatt
    41281 gttgacccta tagatagcaa tcgcatatac cggccatcgc tttcactttc gtatagcaac
    41341 gatggcgggc agtcgtttag cgaagcatcg caggaaggtg gctgggtgca tagcgatcat
    41401 catgcattgt ggataaatcc aaaaaataat aatcaaatat ttcttggtac cgatgggggt
    41461 gtgtacatgt ccctcgaccg tggagtaaat tttgtttttc taaacaatat tcctgtttcg
    41521 caattttatc atgtgagtta cgatttgcaa gagccgtaca atgtatacgg aggtttgcaa
    41581 gacaatggtt cgtggtgtgg cccaacgcaa agtcccaatg gtattgaaaa taaggactgg
    41641 acaaatgtag gcggtggcga tggtttttgg gtgcaacccg atttgctcga tcctaatttt
    41701 gtatatgcag aatcgcaagg tggaaacatg actcgtttca acaaaaagta taatgagttt
    41761 aaagatatac aaccttaccc tgctgctaat gaaacaaagt tgcggtggaa ctggaacacg
    41821 cctttatact ctaatcctgg taatcccggt acattgtatt gcggtgcaca atatttatat
    41881 cgtacaacca acaaaggtga aacatgggag cgcatttcgc ccgaccttac taccaatgac
    41941 ccgttgaaac aaaagcagga atcgtctggt ggtattacag ttgataattc atcggccgaa
    42001 aatcattgta ccatatacgc tattgccgaa tcgcctctcg ataaaaataa tatttgggta
    42061 ggcaccgatg atggcaattt gcaatacacc gatgatggcg gtaaaacatg gaacaatgtt
    42121 accaaaaata ttacgtgtgt gcctaccttt acatggtgca gcagtattga gccttcgcca
    42181 tgggataaaa atacagtgta tgccactttc gaaaatcata cccgtggcga ttttaaacct
    42241 tacattattg tatcgaccga taaaggcaaa acatggacaa accttgccac tgccgatgta
    42301 aaaggctttg cacacattgt acgccaggat atagtaaaca aaaatttatt gtttgccggc
    42361 accgaaacag gaatgtttat atcgatggat ggaggaaaag catggatagc atacacgagc
    42421 aaaattcctg caactcctgt tcgcgattta aaaataaacc caaaaacaaa cgaccttatt
    42481 attgccacac atggccgtgg tatttttatt attgacgacc ttactccaat tagaaattta
    42541 acgcaaaatg ttttagatca ggatgccgca atattaacca ctcgccctaa ttatttgtcg
    42601 ctaggtcaat ttggttccgg ctttccaagt gcgggaggtt acagtggccg caactttacc
    42661 gaagaagccg ttatttatta ttaccttaaa gataaagtga tgacaggtga tgtacgtttg
    42721 gaagtgtatg ataaaaatgg tgtgctgctc gaaaaaattc caggcagtaa acgcaaagga
    42781 ataaaccgtg ttacatgggg catgaaactt aaagcgccca aagtggcaaa aggtataagg
    42841 ctcgatttca gcggttttat aggcccgctt gcaaccgaag gcgagtataa tattaagttg
    42901 ataaagggag acaaagtgac cgagggcaaa atgttattga tgccattacc aaccaatcca
    42961 catagtaaag aagaccgcga cctggcacac actaccgtta tgactttgca tagcatgtgc
    43021 gaagaactgg cattgatgaa tcatcaagtg gtttgtgctc gcgatagtgc caaagcactt
    43081 ttgccaatgg caaaaaacaa caaagctctt acaaagagtt tgaatgcttt aattgctaaa
    43141 atggaaacac aacgtgctac ttacattgca acaactgcag gaactgcaat aacaggcgaa
    43201 gaaaaattgc gcgaaaaact cagcagcctg tatgtggcgg ttgcatacta cgaaggtcgc
    43261 cccaccgact cacaactcga ccgcacaagg gcattccgta aagaacttga cgatgctgct
    43321 gctgctgccg ataaaatttg gaaatcggat ttatcgaatt taaacacgca gctcactaaa
    43381 gccgggttat caaatattgt attgttgagc aaagaagaat ttcaaaatat taagttgtag
    43441 taaaattatt tttttactcc tgacgcctgt atactgtaac tgtatacgtt gatacattat
    43501 tacggttgtc ttctacacgg agcgttaatt tattttcggc agcgatcaaa tattttgttt
    43561 ctatatagag cattttatac ttggcatcgt gcttcacaat tagtttggat tcgttatttg
    43621 tgagcgaata attatcaatg cccgaaatat cttcagcata tcgaaacttg agtgtatcgc
    43681 tattcgaaat aatggagtcc gatttgaaaa gtggctttat cgttggcgca acagtatcga
    43741 cagccaatgc aaatgcacct aatgtttttg atgtggctac aaaataattt ttattcaatg
    43801 tgcctccaat atatgcgaaa ctgccgtctt tattcatacg catcatcaca agttttggtt
    43861 gcagttgttt cgaaattcca ttgacacgaa tagaaatact tgccgattta ttgagtggaa
    43921 atgaggcatc gttgaaatga taaacagggc tcaccaattt tttttcgcgt ttcgatttgt
    43981 ggtattgcaa atagatattg tcgaacaggg cattttcgtt aagttgaaaa atcacttcac
    44041 cgttttgaaa tttgtagggt ttgctgtaag gcaaaaatgt ttttgatcct tgcatcgcca
    44101 ctttttttat tttcaaatta ttggcaacaa aataaaatgt gagtgctgat ttatttcccg
    44161 cttcatcttc cagcaccagc aatgctttat gaacttcctt actgtttaaa tatactttac
    44221 gctcggtgct gttacttttc atggtgctga atccattgtt gggcatgcgg tacaaacgtt
    44281 cggtagtgat gttgcgaaag taatcgcggc tgtaatctat gtaggcattc acatatcgtg
    44341 tttcgtcaaa gtcgaaagag tctaaacgat tctgccaaac tatgtttcca tcaatggtga
    44401 ataacacatt ataaatgccc atgctattgt acgtattatt cattttatcc aaagcttcga
    44461 atccaaatcc ggggcgtgga gttaccgaca aagtatcgcg aatatagtat ccaacatcat
    44521 ccttacgagg ctttagttca tcaactacat cattaccatc atacacaaca agcttcgata
    44581 atattggtgc aatagtatct tcaatggcaa aattaaaaag gcgcggatta atggtttttt
    44641 cggtgtcggt atttcgtact tcgaaatgca aatgcggccc tgcacttcca cctgtatttc
    44701 ccgaatatcc tatcacttca cctttcgaaa ctttgtataa atcatcttcg ggataccagt
    44761 cgagttcgaa ttttattttt ttcgattgct caaaataaac aaggctatca ataaaatcgt
    44821 tgaacctgcg aaggtgacca tacacagttg taagtccatt tggatgctgt atgtagatgg
    44881 cattgccaaa tcctacggca ctcactttaa tgcgggcaat aaatccatcg gctgtggcca
    44941 atacattttt accttctacg ctgtcggttt taaaatcgag acccgaatga aaatgtgaac
    45001 cacgtatttc ggcaaagttg ccggccagcc gtaactttat atctaatggt gccgaaaaat
    45061 aatttttatc ccattgcgct tgcataggtt ttgctcccaa aaacagaacc accatcataa
    45121 gatacctggc cagcaaaagc aagagtgacg aacatttttg aaaatacata attttagaaa
    45181 agtgttaaat tgcccggcaa aggtatttaa aaaactgacc ttgtaaggcc tcaaaaccac
    45241 gttagtattg gtttacaaca aaaagcccgg cattgtttgt ttgcttgaaa aaaagtttac
    45301 ctttgcagcc ccccaatctg cggagacttg gggtatattc aaaaaattag tttaataatt
    45361 taaaaaacaa aaaaatgcct gcaaaattaa gattgcaaag atttgggaaa aaaggccatg
    45421 ccttttatca cattgtggtg gctgatgcgc gtgctccgag ggatggcaag tatattgaga
    45481 agttaggttt gtataatcct aacaccaatc ccgcgactat cgagctcaac accgacaaag
    45541 ctttaacgtg gctccacaac ggagcccaac caaccgaaac ggtaaacgcc atccttagct
    45601 ataagggcgt gctttaccgc aaacacctta acgaaggagt gaaaaaaggt gcgcttacac
    45661 aagaagctgc cgatgccaaa ttcgaagcat ggcaaagcca aaaagctgcc aaaatcgaaa
    45721 ccaaaaaatc ttctttagca attaagaaat cagacgacct caaaaaccgt atggtagccg
    45781 agtcgaaagt aaaagaagac cgcttgaaga agattgccga agcacaactg aaagcttttg
    45841 aagctccggc tgctgaggct gctgaagaag cacctgcggc tgagtagttt aattcttgtc
    45901 aattctgtca ttgtaaggaa cgaagcaatc tctgccagtc attagtcatt ataaattctc
    45961 attattaatt gactttattt ctgtaagaga attaaactcg gcttttagtt tgccaatcaa
    46021 ttgtatagtt cgttgtttac catctttact acgcatttgc atagttgtga aatgactaaa
    46081 caatttaata acacaataac aaataactag catactatac aaaacctgtt tatttattta
    46141 agcaggtttt tttattttac actatggaca aaaaaaatct ctttcaactt ggtaccatat
    46201 tagggccaca caaaacgaat ggggaaataa gcgtttttat tgagagtgac aatccttcat
    46261 actataagga tgttgaaaat attcttgtgg aaataaatac aatgcttatt cctcacacag
    46321 tagaaaaaat aaaaataaca ggcgatactg catatgtaaa actcgctgag gttaataata
    46381 ttgacgaggc tacggcatta actaaatgcg atgtttattt gccacttgat caattaccgc
    46441 cacttaccgg gaagcacttt tattttcatg agattattgg cttcgaagcc cgtgacttac
    46501 atcatggttt ttttggcatc gtaaagcaag tgtacgattt gccgaagcag gatattattt
    46561 cgattgacaa taacggtaaa gaagtattgg ttcctgttgt tgacgatttt atactctcca
    46621 tagaccgcga aaaaaaacaa ttgcatttcg acctccccga gggactttta agcgtatatt
    46681 tggattagtt cgtttttagt ttcgtttgag gggaggaatg ttcttcgttc ttagttacgt
    46741 ttgcggggag gattgttctt agttatagtt acgtttgcgg tgaggattgt tcttagtttc
    46801 gtttgcggtg aggattgttc ttggttctta gtttttagtt cttagtttct agtttaggaa
    46861 tgcagtttag tatttttagt tcgtcattgc gaatgtcaca gtgagtattt tcgaactacg
    46921 aagcaatctc tgcgtagttt tcggtttgca ccttttcatc tttgttccaa aataaattat
    46981 ttgaattcta ttcggcattt attcgtgcat tccccgacac ttcggtcggg gcaggcgtgg
    47041 caaaaaaaat atttgaattc tattctatat tctttcgtga attcgcggct aaattttttt
    47101 ccgctaaagc caatacgaat tatttaatcc gtccgataaa tcggacggca atgatgtgct
    47161 gacgcgaaca acaactttgt gtctttggtc tttgtgacaa aaaaaatatt tgaattccat
    47221 tcgctattca ttcgtgcatt ccccgacact gcggtcgggg caggcgtggc aaaaaatttt
    47281 tattcgcgga tacacagttt tagggaggca agacattgca atagatttca ttaaaaatct
    47341 ggataatagc tcttgatttg atttatagat tgcttcagtc gttcctcctt cgcaatgacg
    47401 gaagtaacta gataaccgaa taactaaata accaaataac tcaataacct aataactaaa
    47461 taacctaata acaaaataac taaacaacca ataactgaat ctccgaaaaa acattctcaa
    47521 aattccatcc atcaaaattg ccggaactca tcatcaatag aacagcttca tcaaaattat
    47581 ttttcatcag gtcggcttta agcaaattcg aatcggtaaa tattttcagt ccacttttta
    47641 caaaacattg cgccacatat tccaccgaaa tgggttttaa ttttttatgc tcaatagtgt
    47701 ggggattaaa ataaacgtat gcaacatcgg ctgcatctaa cgtgtgtgta tatcctttaa
    47761 gaaaggtttc gttcaagctg ctaaacgtgt gcaactccaa acacgcaatt aaggtgcgcg
    47821 cttcaaactg tgattttaca gccttcacgg ttgcatccac tttcgatgga gagtgtgcaa
    47881 aatctttata catcgcaaat ttttgtgttg aacctataag ctcaagcctg cgggccgcac
    47941 ccgtaaaaga tgaaatcaat tcgtaaaata aagcatcact aatacccaca tcagtacata
    48001 catttcgtgc tgcttccatg ttgagcaaat tgtgatgtcc aaatatttgc attgcatatt
    48061 ctttaccacc gtggtggata tacgtagtgc cgtttcgtgt ttcatgtttt gctgttgcat
    48121 attttatttt ttgtgcaagg attttactct catccatcac ggcacataaa tctttgtcct
    48181 cactgcagta aattattttt ccatcgccag gaatttgttc tgcaaaaatt tgaaattgct
    48241 ttttgtaatc ttcataagtg ggaaaaacat ttatgtggtc ccatgcaatg cccgatatta
    48301 ctgccgtatg tgcctggtat aaatgaaact ttggcctgcg gtcgatgggc gatgcaaggt
    48361 actcgtcacc ttccacaatt ataaacggtg cagcatcggt aaggcgcacc atggtatcga
    48421 accccgcaat ttgcgaacct acaaggtaat cgaaatccat tttagcattt ttcaacacat
    48481 gcataatcat actagtgatg gttgttttgc cgtggctgcc acctatcaca atgcgcttct
    48541 tatttttcga ttgctcgtaa atatattcgg gatagctaaa tatttttaaa cccaactcct
    48601 gtgcacgcaa caattcggaa ttatcaacac gtgcatgcat accaagaata acagcatcaa
    48661 ttccggcatt taatttttgc ggaaaccatc ccatttcact tggcaacaaa ttatacttgg
    48721 caaggcgcga gcgcgaaggt tcgtttattt catcatcgct accgctaact atataaccct
    48781 tgtgatgcat tgccaaagcc agattgtgca tggccgcccc tcctatcgct ataaaatgta
    48841 tgtgcatgtt ttttgttttg tatgtggcgc aaatctataa acactattgg tagtgtgaaa
    48901 tatttattta gatttgcttc gtgtttcgaa acaatttcac cgtgattttt gaatccaaat
    48961 taaattaaaa ctactctaat gaaaaaccat catgaaaaat aaaaaattat tcaacattac
    49021 tctatttctg tttgcatttt gttgttgtaa aaatttgcac ggacaaatat tgttggataa
    49081 gcataggatt atagggggga gtgacaagga tgataatcct ttagttctag cttactcaaa
    49141 caatagttat ctattgtttt gggattcgaa atccaacgat ggagatatta ccggaaatca
    49201 tggcggcaaa gatattgtag tgacaatgct tgactcaaat ttaaatacta tttggcaaag
    49261 atgctatggt ggttcgcttg atgaatcggt aaaaaatgtt tacaaactgg tctctggaaa
    49321 tattttgatt ttgggcacat ctaattcggc tgatggtgac ataagtaaca atattggatt
    49381 ttgggacatt tgggcattaa aaatagacag cgcaggcaat attgtaaagg aaagatgtta
    49441 tggaggaaca acagtcgaaa tcctaaatta tggttgcctt atgaatgata gcgatgttat
    49501 tttatcatcg aaagttgggg tgcatgatta tgatattatt cctggctttc ccaatgatag
    49561 attgtggaaa tttaaaattg atacagcttt gggtgatata aaaaaaagcg ttgtggaata
    49621 tactgagccc gcttatttcg aacaatggtt agtgggcttt gattcaacaa caattagcga
    49681 tagatcattg tctatttatg attattacag ccctatcaga gctctttcaa ccacttatga
    49741 tatcaattta aatattttat ctcataatag ccattatacc aatagcctta gctacattcc
    49801 aaattatatg aaatcaccat caggtatata tttcgagttt gttcacaatt ttttttatgt
    49861 aggaaactat cctaatgggc aatattggag tgatatggat gctattgtct acagtaatcc
    49921 aaattggtac agaacatatt cgggtgcaaa aaatgacata attctggatg gagttttcaa
    49981 cgatagtaat caactttttt ttataggtaa cacacaatcc attgacggtg attttgcgaa
    50041 tcctaatcac tgtcaagaca attttgtaat gcgtgctgac agtttaggta atatccagaa
    50101 tataagtaga atttcagacc tcaataattg tgaccaacta agttacaaaa tttataaact
    50161 aaattcaaaa tatgtaatca cttgttcaac caataaaaag aaaggaaaca attggtcgaa
    50221 ggatattttg attgcacaat ttgatgaaaa aataaataaa ttaagtggac aaatatttat
    50281 tgatcaaaac ggcaataatg tattggattt aaatgagcaa aaaataaacc aacctatttt
    50341 gcatataaat ccatcttccg aaaggccaca aattaacgat ggcaattaca catactatac
    50401 agcaacatca ggcatttata atttaacaat agacagcatc aaatactatg tgcaagctcc
    50461 tgcaaatcag acagcaattt tcttaagcga tagtggagaa ataaaaaata aagatttcgc
    50521 ctttcaacca tcaggtattt ataatgattt agaagtatca attactaaaa taaattcttc
    50581 cgacacaagc aaagtggcca tctttaatat taatgcaagc aacataggaa catcagttat
    50641 agattccaag ttaagttttg aatacgattt tatatacaat gtgaacacaa ttaaaacaag
    50701 tattacccca gattctatta taggaaatac tttgtattgg gcgttggatt ccttgctacc
    50761 cttaacctca atgaatattc ttgtttcctt ttctattagt gatagtgcta ttacgggcga
    50821 tagcctaaca gtaaacttaa aaggttcaat aacatcgcag gattttgata ctgtcaataa
    50881 tgtaagtagt gaagtatttc atttagaacc ctacggaaat ggcaatgaaa tgatcgtaaa
    50941 tattgacacc ataattacca accaaaatta tgattatcct gaattagaat actcaatatg
    51001 ttttcagaat tcaaattatg atattaataa gaatgtatac ataaaaaacg atttacaacc
    51061 tgaatacttt gatatgagca ctttcaaatt tgtagaatct tctcatcctg taaaaatttt
    51121 ggttgatgaa cccacaggca aaatctggtt tgagtttaac aatctaaatt tatcaccagt
    51181 atacatgaat gaattactca gtcatggctt tattaaatac agaataaaga taaaagactc
    51241 aacatctctt ggaacaattt tgattaatga ggcggaagtt tcctttgatc aaaatttaaa
    51301 gcaacctttg gtttctgaag ttcacatttt acctatttgc tcttcttctc ctgattcaat
    51361 ttatagctca tccggttcac acaaactttg cgataatgat caaatttatt tatcagtttc
    51421 aggtggagca ttaagtaaag atggcaattt ttgttggtat aaggattcgt gtagtggtac
    51481 tcctattgcc aaaggcgaaa cttattatac agggcatata actcaaagca caatgtatta
    51541 tgttcgagcc gaagattcat gtggcattgg tagttgcatt tcgttttatg taacagtatc
    51601 tccctattat cctattctat ttggcactcc aaacggggtt tctgcgtgta atgattcgtt
    51661 gttgatttac ggccatgcac ttgcctcagg cactacgaat tggtacaatt ctggttctct
    51721 tataacttca gatatcgatt cgttctacgc gaagatttca ggttattaca cattcgaatt
    51781 tacttcggac agtggatgta aaagtgtaaa atcaacttcg attgacattc ataaaataaa
    51841 agtttcattg acgagtgata gtataatttg taatggaact aaaataaaac caacaaaata
    51901 tttttcgcac tactcgtgga gcgatggtag ccaaaatcca tatttggaaa ttgatacacc
    51961 gggtacgtat acagtcactg ttagtgataa ttacgggtgt tccgcttcat catctacaac
    52021 agttggaaca gtaataccaa gtcctgtttt ggaactaaat ggaaattgcg acactacggt
    52081 atgcaattcc attttactag atgcacaaaa cccagggtca tcatatttgt ggaacaatgg
    52141 ggctactact caaacttatc ttgcaactaa ttcaggcctg tatagcgtta ttgtgacagc
    52201 attaaatggt tgcacaaaat cagattccat tcaggtggac atatcttcct ttccaccaaa
    52261 tatgcttggc aatgatacta cagtttgtgg cactacattt tcgcttactc catcgttttg
    52321 ggggccgtct tttttgtgga gtaccggtca aacaactcaa tctatttcga ttgacacttc
    52381 aggaacttat tctgttactg ttaccgacta cgtaggctgt acggggatgg atgaaataaa
    52441 tttgattata agtcctaagc cgcaagtttt ctttgttcca tttgtgagta atacaatttg
    52501 caaacaagga ggtttaataa atataaacca aggcacacca acaggaggta agtactactt
    52561 gaatcatatg ctttaccaaa atagcgtaat tgaccccact acattgcctt tgtatgttaa
    52621 tttaatcgct tacaaatatg ttgatagtgt tggttgtagt gactctgttt taacaacatt
    52681 ttggatttcg ccttgtgcct ccatcaattc aatggatagc gaatcaagca taagcataat
    52741 cccaaatccc gcttccgatt attttaccgt tcatacatca atatcgaatt attgtgtgaa
    52801 attattttcg gcaaaaggag aattggtttt tgaaaaaagt aattgtaaaa acgacaccag
    52861 ggtaattact aagatgtatg atgccggatt gtacaccatt caattaattg ctgaacgcgg
    52921 aactattgct ttaaaggtat cggtggtgaa aaattaattc tcctatttat ttcaacaatg
    52981 agtttgtaag caatattata tttgaaccta aattatactt tcgctgcaaa tatttttaac
    53041 ttatgaaact tcacacgata aataccggat acttcaaact cgatggtggc gccatgtttg
    53101 gcgtggtgcc aaaatctata tggcaaagcc taaaccctgc cgatgataac aatatgtgca
    53161 actgggctat gcgttgtttg ttggtagagg atggtaaccg attaatactt attgataatg
    53221 gccttggcga taaacaagac aaaagatttt tcagccacta ttatctgaat ggtgacgata
    53281 cgctcgtaaa atcgatacgc aatgccggat atggattgag tgatataacc gatgtaatac
    53341 ttactcattt gcacttcgac cattgtggtg gcagtgtgat ttataatagt gatcgtacaa
    53401 aattgcaact tacttttccc aatgctacat actggtgtgg ggcagaacat tgggatttgg
    53461 tattgcatcc caatgcgcgc gaacgtgcaa gttttttgaa agaaaatatt tttcccattg
    53521 aagaaagcgg ccatctcaaa cttgtaaatg acggtgccca aatttcgcct tcaataaaag
    53581 taatgatgag caacggacat actgcaggca tgattcatcc catgattgaa tacaaagaca
    53641 aaacaattgt gtttgttgcc gatatgatac cgtcatcgca ccacataccc gtgccttatg
    53701 tgatggggta cgatacacgc ccgctgatta cgatggacga aaaagaaata tttttgaaaa
    53761 ccgcagcgga gaaaaattac attttgtttt ttgagcacga ccccattgta gaatgttgct
    53821 cccttaaact aacagaaaaa ggcgtgcgca aaaatgaaac ttttgccttg agcgaaattt
    53881 tatgattcgt gcgtgtaaca atatgaatgg ctttacatca aaccaagcaa accaccttta
    53941 gatgagcgct gacaaacgtg tcttgttcaa cgatatttat actagaaaca aaggcaagat
    54001 atttaaactt tgccttggct atgccggtga tcgcgatgtt gccaacgacc tgatgcaaga
    54061 tacatttgta aaggtgtgga atcacctcga tacatttcgt aacgatgccg cggtgagtac
    54121 atggatatac cgcattgctg tgaatacttg cttgggatat ttgcgtactg caaaaaacaa
    54181 accttccgaa gcactgaacg attacattat tgaaacaaaa caagaagaac taattgatac
    54241 cgatgtaaaa gtgcagatac tatacaaaag cataagcaag ctcgaagaaa atgacagact
    54301 cataatcact atggtacttg atgatgtaga atatgctgaa atagcagcaa tatcaggcat
    54361 ttcggaaggc aatttgaggg tgcgcataca ccgaataaaa attcgcctca ccgaaatata
    54421 taagcgtact gagcaagaaa tggagactgc atttatgagc aataaaaatt gagaagatgg
    54481 aatttgacga tataaaaaac ttgtggcaac atcaggcaga gcaaacactt cctccgcccg
    54541 aaaacgtttt acaaaaagta aagttgcaca agcaaaaaat tttacaaacc ttgttgcgca
    54601 ccgttattca acttacagca acttttgcgt ttatattatt attggttcta acaattaaat
    54661 tcacctctcc tgtaacgtat ataggcattg ttgttatgtt actatgcatg tcaatttatg
    54721 cttacataac ctataaacat tacaaattat tatcgaaaga tttttcgaca ctgccggcaa
    54781 atgaatatat cagcacatta aagcaaacct atattgccag acaaaaattt ttgcaaaacg
    54841 gcactctggt ttatgttttg ggtttaagtt ttggcctgtt actctacctc atagagccaa
    54901 taagtttttt atcacctttc tggaaaatat tttcgatagt cgcaaccatt gcatggatat
    54961 tgtttgccac cctctattta tcaaagcgtg ccatgaaaaa agaatttgac aggttcgatg
    55021 aaatgataaa acacctgaat gaattgaata gccgcatgta attaataaaa tttatcgcct
    55081 ttccaatttt gtgggttcgt tgctatataa ttttgtattc gttcaaaaga ttttgcatca
    55141 cgaattatat gatcatgaaa acgcggctgc caatcaaacc ctgcatctat ttttcgtgca
    55201 ttgattgtac aaattcgttt gtattgattt ataataacac caattgttcc cggtttccat
    55261 ttttcgtttt tgccaccgat ggtggtagag acgcccaaat tgggcgtctc tacgggcaaa
    55321 acgcgcgtaa cgggcgaaac ggccggcacg gtcgattccg aaaaattcga tttatcaata
    55381 atcaatattc catgggtatg atttggcatc acaacaaaat tacctaattc aataaatgga
    55441 aaatgtttag gaatctccat ccaaaatttt tcggccaatt ttccaatttc ggtcaattgc
    55501 attatttggt ttggtggggt tgacgcgggc gttgnnnnnn nnnnttcgtt tttgccaccg
    55561 atggtggtag agacgcccaa attgggcgtc tctacggggg aaacggacgc aacggacgat
    55621 tcgggcgcat ccacaatttc gccaaaaaaa tgtttccggt ttttggtaca aatggttata
    55681 aaataaaaac cctcgctggc ataatcccac gtttgcaaac gggcagatgg tattcgatat
    55741 ttattcttga atttatcggg cattgtttta tttattgtga gactatttgc aaataatttt
    55801 ttacacattg caaa
//
LOCUS       DAORQA010000004        54904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_97_length_54904_cov_32.492826, whole genome shotgun sequence.
ACCESSION   DAORQA010000004 DAORQA010000000
VERSION     DAORQA010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 54904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54904
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97_length_54904_cov_32.492826"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1685
                     /locus_tag="PK736_00825"
     CDS             <1..1685
                     /locus_tag="PK736_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC31965.1"
                     /translation="MADMANDFLDKEVTPYLDRIDAQEEGLMQSILDKAGALGLLSVS
                     LPEEYGGLGKDFTTGMLITEALGAGHSFSVAMAAHTGIGTLPILYYGTDAQKKKYLPK
                     LATGEWKASYCLTEPGSGSDALAAKTKAILESDGKNYVLNGQKMWITNAGFADLFIVF
                     AQIDGDKFTGFIVEKNAVGLSLGNEEHKMGIKGSSTRQVFFSDCKVPAENVLGEIGKG
                     HLIAFNILNIGRAKLAAAALGAAKKVCSTSIAYALGREQFKIPIAKFGAIRYKLAEQA
                     IRVFACESAMFRISDYIDEKEKALIAEGKNFSQALLGAAEEYAVECAMLKVYGSETLD
                     YVVDEGVQIFGGYGFSADYPMDRAYRDSRINRIFEGTNEINRLLTVDMLLKRAMKGQL
                     DLMTPAMNVQKELMEIPDMSEGDSSQPFFAEYKAIANFKKATLMVAGSAVQKFMMQLA
                     KEQEILMNVADMAINLFVAESTLLRIHRLLQLKGKDAVTVHTQMLQVLMNDTADRINI
                     AGKNAIQSFAEGDEMRMMMLGLKRFTKVNLVNTRDGRRAVAKALTDNGKYCF"
     gene            1692..2339
                     /locus_tag="PK736_00830"
     CDS             1692..2339
                     /locus_tag="PK736_00830"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRC31966.1"
                     /translation="MTLPFPDFIIKLQHALTFPLPGIEAHKEMVPVGRVAYEQLQNQI
                     EEYRKGAVLIHLCFDNDEWKVIFIQRSTYDGVHSAQMAFPGGKYDDSDGTFQNTAMRE
                     AYEEVQLHAHQINFIGELSVLHIPVSRFTVYPFVSWQHGMPNLVPDVNEVTSIHILPL
                     ADFYNDNNRIETEVTLQQGVKKNVQAFNIQNNIIWGATAMIMHEFVTILKGLKVQ"
     gene            2717..3367
                     /gene="hxpB"
                     /locus_tag="PK736_00835"
     CDS             2717..3367
                     /gene="hxpB"
                     /locus_tag="PK736_00835"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006530358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HRC31967.1"
                     /translation="MKAIIFDMDGVLIDSEPLWRKAMIAKFNAVGLPFDDDRCMQTTG
                     LRIDEVTQHWYNLFPWPNVTPHQLAMDVIDEMIRLINIEGEAMPGVYETLKMLHSANC
                     KIGLASSSSMVLIKAVIEKLEIKEYFDAIHSAEFEKYGKPHPDVYIHTANLLNTPLRN
                     CIAIEDSLNGLISAKAALMKVIAIPEAHNHHHAKFIIADKIIHSMQELTMEIINEL"
     gene            complement(3920..5596)
                     /locus_tag="PK736_00840"
     CDS             complement(3920..5596)
                     /locus_tag="PK736_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRC31968.1"
                     /translation="MNHTANEEHEKLNFIEQLVEEDLKTKKHDGRLLTRFPPEPNGYL
                     HIGHAKSICLNFGLALKYGGKTNLRFDDTNPEKEETEYVESIKHDIKWLGFEWSEELY
                     TSDYFEQLYDFAVILIKKGLAYVDDQTAEQMAAHKGTPTEPGTLSKYTRRTVEENLDL
                     FTRMRAGEFAEGEKTLRARIDMTSPNMHLRDPLMYRIKKDHHHRTGDKWCIYPMYDFA
                     HGQSDSIEKITHSICTLEFEVHRPLYDWYIKQLEIYPSQQTEFARLNLNYTVMSKRKL
                     LRLVQDQYVSGWDDPRMPTISAMRRRGFTPEAIRNFCDTIGVARRENVIDFGLLEFCV
                     REHLNKIALRRMVVIDPLKIVIANFEEGKKEILSAENNPEDPEAGNREMIFTKEIFIE
                     HEDFMENPPKKFFRLAPGGYVRLKNAFIIKCDEVIKDDADKVTQLNCTYVPESRSGHD
                     TSGISVKGVLHWVSATENITAEVRLYDRLFTVENPDNQEKEFLHYVNPDSLKIINKAY
                     IEKHFALDSIDSKFQFLRLGYFCFDKDSTPQHLVFNKTVGLKDSWAKISS"
     gene            complement(5656..7005)
                     /locus_tag="PK736_00845"
     CDS             complement(5656..7005)
                     /locus_tag="PK736_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRC31969.1"
                     /translation="MNKFLVYQSIALAIALAACGKRTPTFVEVAPIIYKNCSGCHRPG
                     QAGTMALLTYSDCKKYAHTILYTTQSRFMPPWPADHNYVHFIDEKYLTEEEIKIIADW
                     AEGGAPMGDSAAIPPVPDFPKGSELANPDMVIACPPFKIKGDNREKFIMVKVPFELPN
                     DTFIRSIEFVPGNKKLLHHMNGHMIVFDNNKSSKDLYRQPFIIDRDTAYEISESAHNL
                     NLLYDDGTTPIFIGSVANYLPGSSGIIYPENIGGYTSHKRNALLLRDIHYGATTVEQW
                     DSSYFNIFFTDKKPLRPTREIQLGTLGISPIVPPLVIPPGKISTYKTNAVVPVDISLL
                     TINPHMHLLGKSFWAYAIAPNGDSIRIVKIPKWDFRWQYNYTYPKPLIIPAGSTIYVE
                     GVFDNTDKNPNNPFFPPRTVREPIGGNMKTTDEMFQLIMTYLPYKKGDENIDLAKNK"
     gene            complement(7041..7622)
                     /locus_tag="PK736_00850"
     CDS             complement(7041..7622)
                     /locus_tag="PK736_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRC31970.1"
                     /translation="MKIFAVQIFPFLIALCLSISLACCSCNKTSNLEISNLNHTSSTL
                     QNQLSSNYTVLVFLSPECPICQGYSTTLNNLNLEYSKSGIKFIGLVPGDGFSSDTIKY
                     YQAFYKINFDLFIDNQKLATNKFHALTTPHCFLINNKMEIIYEGLIDNYAADVGVKRQ
                     VVTQHFLHDAIEAVLQQKEIAVKKTKPIGCFIE"
     gene            7949..12730
                     /locus_tag="PK736_00855"
     CDS             7949..12730
                     /locus_tag="PK736_00855"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31971.1"
                     /translation="MQGTLGSQITIPPNYFGFNGANVVRAGNPSYNTPWLIDSIGLFG
                     PEIIRYPAGVPSGFWDWQKGTLYQNPPGGFLLPREYDGYIFKTNTLDMYKSMVDLLGL
                     TPLQNINVLCSDPYYETARLYYSRGINLPVKYIEFGNELYDDEYPQYLQKWPTANAYI
                     DDMNNFASVLRSFPGSNEFKIAAVGAVNRLGLEPAESRRNTWTSIITSNASADIDAIT
                     LHCYIGTGLDENVALSLSNLPQVLSTPFKRMDDLSNIELAQLRNAGKEAWITEYNVFD
                     KKHCIHSTWAQGLMAASMTLSFLNDSTIKLINFHSMVADGVWGSIFKDSSDLDISTFY
                     NGVTCTTNPPQAKPQQRSGCGNAMLLVGQALKGSASVRKISLTNNPVLYDVYNSVHGW
                     QFDGPIATQGIFLNLSGTIRTVVINQADWDLSGYKFEQISVGPGGPLALITGNATKTP
                     GVNDVETSGFNNFVGQTIVLKPYSVFRLVKPKTGLLMKVVDNTICSGTTTSLVAQGTG
                     NFSWTPMANLISLKPDNSVMQFTATVTNTTVYTVTVTAQNGITSSVTITVYPKPKLNA
                     TATLSNTCKNLPVKLAATFTGGNTSNFNSFIWLPSTEVDNPFSDVVNVNPKKTTTYTV
                     YATDGQCWSSVDSVKVNVSNNADATDSRLVCRDSLPLKLSTKVNKYKYVYNWYKNGVL
                     LGTGKNIFVSPDTITTYMMVMQSTIDSYCVDTDFVTITPITCCGSKDASMLTFGPIIK
                     MSEIAVRIKKYCDSTANGVATLYGVNGLAKEIVINGQLIVDSDYTFTNCSKIKFGENA
                     DVLFPGEGAHLNFQNCKISAAECSGRMWNGFSFSDWTESLTLNNTTISDAKIAIKATL
                     NAQLVITNCVFNNNYIGLDIATMESTFNPVIYGDSFIYSALLPPYENQKPFAGIKLND
                     IIDITIGKAGYPSNVFLNSSYGIYAENAHVYLYNNIFRNILNTNNGDVNEGSAVYANN
                     LNGYGDVTNPNHIWEELSVVAGSTTAANTFDNCFYGINAINHKLKVNYNNFNDVNNAI
                     SCLGCVKKEISITYNFMDSVGRGINLIEPKLSTIDILNNSILTSTLKTPIANVAAINV
                     SDNVIYPSALDIWNNVVTNYGKYGILVTNNFKPSIKENKVYMRMGTTTESVYALNVLN
                     SDSSKINCNFVGSTNTTLSKRIGLSASFCAGSNFRCNVIDKCYTGMEFWGNCNNSTIK
                     NNELRHLAVGLAVGKSGTTSGIIGPQPVNATGKPTHNLFTGQYTRTSGAGNKKGYFSV
                     AATLAINSILAGGKFDLTRYLVLNGLTSTNDSVQVPYPNSKTGTGTAMTPFYTFNTPV
                     TCDVNCTMQTAKLNMNNADDNSDDDFVDVKELLRNNMDALAFEKEPVIQYSLKKNIYA
                     LQNNEGNKYTVAAQLHDITSMMQKLSSVNNDDVEKIVIANSSINTTEKFEVNDQALND
                     LELKYMMKKQKCFTKPQIDFIFSIASQCPVNGGTAVYRARAMYQTMYPTHLFDDENAC
                     NRITENNSTALDNTSLNVEVMPNPTKGLMHIMLSNENETELQMLIADMEGRIVFNSNI
                     AAHSIQKTIDLSALAPQVYMMNIYDNQNNVMCRKKISVVKF"
     gene            complement(12839..16213)
                     /locus_tag="PK736_00860"
     CDS             complement(12839..16213)
                     /locus_tag="PK736_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HRC31972.1"
                     /translation="MKLCLIALLACTIISTNKIIAQDSVVVQTFDFNSTVRKGTFAFP
                     PDTSTWQKILMQYRIRCKKGQVSSSNNALYGCGEWDYSCNTYITDSSLTDSVKSKHNS
                     YDITNFNGTTFSYTYTPVYTYYQYIQKNMVYSVINENLASVGNGATLVNHPLANGVTS
                     GKTQYLYTAAELIAAGVVAGDISGMELDIPQLGAQTDFLRIKIKATSLSQLTQIDNTP
                     GFNQVYFLNTPLSNVGYNRFQFYSPFNWNGTSNIIVEINYNGKSANSGHTANAASTVD
                     AAVTSMTDDYNVKLNGSSYGDAGDINELDSVQKFTLQAWVKINKWKNWAGIFNKDGKT
                     TMELGDVLDRVYCIVRNPNNSYGYASGVLPLNTWTHLTMVYDGTKVSNGKRLRFYVNG
                     AEVALTYSGSIPNKTAQSTQVQKFLSGIDGQIDDVHIWNEALDSAAIAGWMYKKIDTS
                     HPFYSSLLANYEMNEGLDTVLTDVSTYNRTGYLRNGIGWDKFDGNKIFKNLVPVTTRP
                     NINFAQGTYTNTLQDIIITDSLLNNAHTVTSYTVNGTNLDTIYSLQAWQAGYGYVYDE
                     AGAIVDSVMYAADSTVTISILDYYAKWPSRFEIMSFVTPYGNNLDLGLTGKMYEFDVT
                     DFAPVLKNNKYISIERGGEWQEEMDIKFIFIKGTPPAPVLSIRQLWPVTYDNYANILN
                     DYRFEPRTVTMNAAGSYFKLKSAITGHGQDGEFTYRTHYMNVDSGLKDFQWLVKKECA
                     LNPVYPQGGTWVYDRAGWCPGMATDVKEFDITPLVTPGQDATFDYGINVVTGASNYIV
                     NNQIVEYGAASFTVDASVIDIVKPNDKIEHGRGNPVCMNPIVQIKNTGSTTITSLDIH
                     YGMEGSALTTFTWNGTLPFMDTAFVPLNIANWSNTAGTFIAYVSNPNATTDQYAQNDT
                     VRSNYVLPVLYPPGIIVEVKTNNQGSGTKYKLKNDAGTVLVNRLGLSNNTIYRDTLIL
                     PLGCYEFQLTDVGNDGLSWWANTAQGTGYCRIKKIDGTLIKVFNPDFGAEIYQQFGIN
                     LLLGESENNSSSTAQLNVFPNPASDKADILIDFAKSKDAELRITDLQGKIINQHSFKN
                     IISENYSLDCATLSKGIYAITLITADEVKTVRLVIQ"
     gene            complement(16458..16874)
                     /gene="ndk"
                     /locus_tag="PK736_00865"
     CDS             complement(16458..16874)
                     /gene="ndk"
                     /locus_tag="PK736_00865"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRC31973.1"
                     /translation="MSNITFTMLKPDAVGNGHTGAILDHIIKSGFGIVAMKYLQLTPE
                     RAGQFYEVHKARPFYNDLVKYMSSGPIVAVILTKDNAVEDFRKVIGATDPAKAEPGTI
                     RNLFAKSIEANAVHGSDSDENAAIEAGFFFNRFERF"
     gene            17009..17839
                     /locus_tag="PK736_00870"
     CDS             17009..17839
                     /locus_tag="PK736_00870"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRC31974.1"
                     /translation="MHKITVHGHRGARGYLPENTIESFLLAIDMGATAIEMDVVLSAD
                     NRVVVSHEPWMHHAICTKPDGTFVGLEEEKSLNLFKMNYNQIAEFDCGKRGNVKFPQQ
                     KHLPAQKPLLDDVILAVENYTRLNKLNPIIYNIETKCARSTEGIFHPAYDIFTQLVYD
                     CCAKQNVIDRCIFQSFDERNLIYLKNLDKRIQLSLLSEYEINLELAIESMGFTPHYFS
                     VLHTLLNEELVALIKKLGMEILCWTVNSTEDIRRMKQLGVDGIISDYPDRVINILAED
                     "
     gene            complement(17894..19582)
                     /locus_tag="PK736_00875"
     CDS             complement(17894..19582)
                     /locus_tag="PK736_00875"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRC31975.1"
                     /translation="MKKIRLILMVTMSIYACSLNAQFVWTPQVSNVTAELRGVYFTNS
                     NHGVAVGAPTGIGLPSTIIRTDDAGNIWTPIITAFTDTLRAVCFTDDTTGFASGANGR
                     ILKTTDGGTVWDSVSTGVNSLLRTITFPTPLIGYAAGGGGVMIKTIDGGATWTTLTSG
                     VTQDLINMRFANVDTGFAVSSNGTFTNGFIIKTTDGGATWTSVYTDPQGLLGLVVANA
                     DTIYAGGGSQVIVQSIDGGTTWTNQYTGNTGSNIRSGFFVNSTHGFMVGDVGAIFETK
                     DAGVTWVNVGTQTNGNLGMHFPTADTGYVAGNLGNMLRYNPTCLPFLTAPAQIFGVDS
                     ICGKDSVSFYINTMPGANSYYWKVPPAATLYNQGDTLVWVKFNNIVGKISAAGVNQCG
                     PGDSLETSVYLYPPTPPAPVSKNLDTLFSNVNAISYQWYVNGNIIPNATLSYYVFTAN
                     GNYSVLITDLNGCTSISIPYAVNNVGISEIYENEMYEVFPNPFTNYIEINVKNTKAVN
                     AEINDLLGKVLVSVQLSSSTKNIISTNNLAGGIYLLKIFDTNKTQIGVEMILKK"
     gene            complement(19718..21562)
                     /locus_tag="PK736_00880"
     CDS             complement(19718..21562)
                     /locus_tag="PK736_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31976.1"
                     /translation="MKLVIIFLLFMNMICHGQQNKISYPSYENVLNNFFEKYSVKDLP
                     GRYGITDELKFEKKSDGWHVVVCDAMNNNVFKDELFWSTESASFLKINFESAADTKEN
                     KTNRYAFDDNWKIHNFNICPYYGYIGWDWDVIEDFKNSNNLSDSVLFGLGRAYSSYAS
                     NLLNNNTGYALKKNQFVLPNTRNCMSPQQLETYREYRHAAINIYKKLFERNPQFETIV
                     GEMAIKYSNENLVSFLDLKIYQNEEEARKELKPGLYTPFYINAAKNYLNSCEPNAILF
                     TNGDNDTYPLLYVQAMYGFRTDVLVVNLSLLNTERYINYMRHPYAGAVPLSFSFTEQE
                     LENKNLEYALFAKNEESEISLSDVLKKLHEVNNDQATTEKIKKISSRNFTFHTDAFLL
                     DWKYSKSYLSLSDILVLDIIHSHMGKRPIYFAGTIGGENFLGLKNYLSLEGIVYKLST
                     QKFENSDYYTGGINTNTLFNIVINNFEWAGMNTTSKSKTLICKTLRLTFYRLAYELIK
                     ENKLDSARQVLNMCFETMPDNYEPFDFITLNLIECLYALHDTTKAVEISRLLLQSAIN
                     GRFDEVKSTSSSSTDYKQAIMGGLRKLAYNNNQTSFATELDQIIYYWH"
     gene            21839..23572
                     /locus_tag="PK736_00885"
     CDS             21839..23572
                     /locus_tag="PK736_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC31977.1"
                     /translation="MNNRLVLILFLLISTVSYSQVSLQWQFYGDTPEESHFIVADSHE
                     NVYTLGAYGGLRKYNHTGDTVFYNTDTTQYEAKAIDIDGNDNIVTVGTYFGTTQDYRI
                     LVQKFDTSGNKIWATAINTPNGNREFASAFTFDQINNIYLTGWDGDQFFGKFFTAKVS
                     SAGAVVWTNSFPKNGFDPYYGLDIAVHDTNSVYATGFVDENGTTNDMLLVKYNSNGDT
                     VWSRKFGYYQTFGGQWTTEDNGTEVETDQLGNVYVISKDIFGNHDVLIKYNPSGAKQW
                     AKEVSLSPTQISFMSIQNNNIYMAGNADIGFGLTASLAIKMDLNGTVIFDSTFATKTA
                     QIADAKVYNGGIVMTGTFTPCNGCNSDIVTMYFDSVGNSSWSNVMNTSNGFFTDQAYG
                     LTTDNNGGIYITGNGNGSSYWMDTYKLFPCKDIKPAIAKNGNLNTANYIAGSTYTWRD
                     CANDSIVAQGFELFQFVQPYPGNFAVEISVGTCSDTTSCIVANLNTIPKNENEYIIYP
                     NPAVDIFNVIGISNNNTTATLYSADMREIDTFTLTPESAAIDLRNLCKGIYFLSITNS
                     FSNRIIKLIKE"
     gene            complement(23905..24372)
                     /locus_tag="PK736_00890"
     CDS             complement(23905..24372)
                     /locus_tag="PK736_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31978.1"
                     /translation="MEEIAKILFAVHCYKCSSINNTEDSDLLWVELIEKSDNKEEIDK
                     KFFADMATFVILNTTNSYNPMENLAIILSRIHSKKLWPFYYENEPICQHDTEEWGGYF
                     WHQLKETPAIGKVSKTHFRNIAVEILNKYPLVRPEYMRPNPASDTPTPDGGSL"
     gene            24866..25090
                     /locus_tag="PK736_00895"
     CDS             24866..25090
                     /locus_tag="PK736_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC31979.1"
                     /translation="MTINAQRKNIIDQLQLINDKDLLSTIQTVIEYGVKKSNFQVISM
                     EEQTRILKIKSSNREEDFIDEEVLDKLLDS"
     gene            complement(25928..26164)
                     /locus_tag="PK736_00900"
     CDS             complement(25928..26164)
                     /locus_tag="PK736_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015230742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRC31980.1"
                     /translation="MNTLTINKSNNAVDVKFTENKMMLFMEDGREISFPLEWFVSLRN
                     ATQAQLNNWRFIGKGEGIHWQDLDEDISVQLLLK"
     gene            complement(26142..26309)
                     /locus_tag="PK736_00905"
     CDS             complement(26142..26309)
                     /locus_tag="PK736_00905"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRC31981.1"
                     /translation="MHVESTGRTAKFNLGVVELVKSSGFNSKELKEIRIIVEGNKNIF
                     NIKWNEYFNNK"
     gene            complement(26635..28023)
                     /gene="lpdA"
                     /locus_tag="PK736_00910"
     CDS             complement(26635..28023)
                     /gene="lpdA"
                     /locus_tag="PK736_00910"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRC31982.1"
                     /translation="MKYDLIVLGSGPGGYVAAIRASQLGLKTAIVERESLGGVCLNWG
                     CIPTKALLKSAQVFEYLQHAADYGITVEGGKADFEKVVKRSRGVADGMSKGVQFLMKK
                     NKIDVIMGSGKLKPGKKLEVTDAAGVVQTLEANHIILATGARSRILPNLPQDGKKVIG
                     YREAMTLPQQPQSMVVVGSGAIGSEFAYFYNAMGTKVTLIEFMPAIVPVEDEEVSKQL
                     EKSFKKSGITVMTDSSVESVDTSGAQCVVKVKTKKGEQEITCDIVLSAVGIATNIENI
                     GLEETGVITDKGKVVVNDFYQTNIPGVYAIGDIVKGQALAHVASAEGIICVEKIAGHH
                     PEPLNYNNIPGCTYTSPEIASVGYTEKGAIAAGFEIKVGKFPFSASGKASAAGAKDGF
                     VKVIYDAQYGEFLGCHMIGANVTEIIAEAVVARKLETTGNEILKAVHPHPTMSEAMME
                     ATAVAYGEVIHL"
     gene            28191..30680
                     /locus_tag="PK736_00915"
     CDS             28191..30680
                     /locus_tag="PK736_00915"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRC31983.1"
                     /translation="MNYKKLFFLFLFSFLFKFQSLSQLLSNGFYKVEVVNQNPALKAA
                     EGLTFDKNGRGYIWDKRGYVFILETNGTLNTTPLLDIKEEVANFGDNGLNGFALDPDF
                     LTNGYIYAGYSVDRYYYDYFGLPGYDPDSSFIDTLSFGRITRFKADISTNYTTIVPGS
                     RTILLGDSIHNGMPVLYFSHSNGNLVFGNDGSLLAAVGDAAAYTTLDTGSNSFTYWNQ
                     GLVDNVIEPKHNVGAWRAQMLTTLSGKILRLDPQTGDGLPSNPFYDTTDARSPQSRIY
                     SLGYRNPFRFSIVKNTGDTDITAGNPGTLILGDVGWSKWEEIDIIKTAGLNAGWPYFE
                     GLATNPFFEVSNEVNEDEPNPLYSPGICDFQYFRFRDLLQQESNLNPPFPNPCDSSIN
                     MTQKHQSYHFRPTLEWRHNQKLTRTGIFSGGTGKGIPININLANSPIKGEMFEGNAAV
                     GGFVYQTNKVPLEFQNAVFFGDYGEGWIRYMRADANLNIDSVFSFDTTASTIVCLTYN
                     EIDACIYYVSFPDKIYKICYPGLVNNPPVSLPTSDTIYGPSPLTVNFDGTASFDPETG
                     PLTYQWDFADNGAVSNNAIDSHTFSPGPGFPVLYNVTLTVTDTGMLQNTKHLLIHVNN
                     TPPKVDITSPLIGTTYSTSIPTLINMTAQVSDLESPNVQLSYSWKVEKYHNSHTHPEC
                     DETDPVTYCILPPEVCLPNDTVWYKVSLTVTDPQGLKGYDFVGIPPLCNVGLNNLLFQ
                     QNKEILMFPNPIENDQFDIKFFDEKIRTVNKKDVQIFTSNGDEFSPKNYTFGFNNNST
                     WSVKLNHLPSGLYFIKINTGTNFVKAKFIKL"
     gene            30769..34479
                     /locus_tag="PK736_00920"
     CDS             30769..34479
                     /locus_tag="PK736_00920"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRC31984.1"
                     /translation="MLYKNVKATYFTALVLFFLITKCYGQGSPVLTPLFSDNLVSSGW
                     NQVVGFQYDSLGRMYVWEKSGKVWFVDANGVKSTTPVINLSDEVGNWRDHGLNGFALD
                     PNFNTNGYIYLYYTVDYYALKNKGKLNYSTTTNEYLRATIVRVTRYTCDAANDFKTAL
                     ISTRLVLIGKTIKDGIPLLHTSHSGGSLVFGDDGSLLISTGDGSSFNQVDTGNATSSY
                     WSNGLLDSIIRPAENVGSFRSQMLDSYNGKILRVDPATGMGLPSNPFYDPNSPDGPQS
                     KVWCLGLRNPFRFTVKRGSGSTDITAGQPGVLMIGEVGWQVWEEINVSKNGGENFGWP
                     FYEGLTPHTGYFSSQVKNLDEPNTILDTVNCGLDFYRFRHLMIQDTLSNDSIFPHPCD
                     ANQQIPKSSHPFTHTRAAIDYRHNTVETRVPVFTNGTANTVKIDDPSSPIVGQQFSGN
                     ASVGGVYNSDIRLSAKYFNTYFHADYGEGWIKNLKLDGNNNIISIDTFAANLGPVVYL
                     TLNPNNGCLTYVHYENEIRKICYTGVVNYPPLAKAQSDKIFGTSPLTVSFGDSLSYDP
                     DGQPLSRTWYFGNGDSSNASNPSYIFTSANNNPITYTVILKVTDALGLTSFDTTRVYL
                     NNTPPIVDIITLPDTFLYTVNNSTILNLRANVFDAQTPTTDLIYKWQTTLYHNNHNHP
                     DPYETDKETTVDISPLGCDGEQYNYSISLTVCDAQGLCGKAEVYAYPDCNPVSTLSGG
                     FTVSSKDVCRNTDIIFTDTTKNATSWKWYFPGGYPSQALTQSPLVNYSSTGTFPVYLV
                     AKNDLTQDSVVRLNYIKVKSLPTITGITNYGNDTVCGPSSFYIRAITTTTNLIFQWYK
                     NGVPIFAAFDDSLLVDSAAKYTVVVTNTNGCSSTFSKTIYNGSLVTLSIATSHTLYLC
                     TSVDSALVSSVNNPAYTYQWYKNGIAINGANNSNYYVKSKGSYYLKVNANGICTNNLT
                     SNTLTFTNRPDVTFSVSGPLTFCNYDSVILASTPCFDCAYKWYKNNTIISSYGTTKSN
                     YKATTNGTYKVKVIDPLGCSLISPNWQVTVNPVFTVAQSGPLTFCNGDSVTLTVTSNP
                     AFKYKWKRNNVWVPNAETNKLTIKTAGQYYCRVTDTLNNCVNRTVFYNIVINCREAGM
                     FDLDNISLVPNPTEGISTLSFIANTNEQNAIISIYNLQGQEVWKENVFVQSGENGFDI
                     DAGDLASGVYEIRISAGNEIYKAIKFVKMDKQ"
     gene            complement(34532..35425)
                     /gene="speB"
                     /locus_tag="PK736_00925"
     CDS             complement(34532..35425)
                     /gene="speB"
                     /locus_tag="PK736_00925"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRC31985.1"
                     /translation="MAALKSLSTKDNFLGIEEKKYYDYSSSKYVIQQVPYEHTSSYLA
                     GSAKGPGAMVKASQFVELYDEELGIETFRKCGIATLPPLDFKNKVDAKAVDYIEQETT
                     RLLNDKKYVVSLGAEHTVTYGFVKAYAAKYKNLSVLQIDAHSDLRDTYHNNKFSHASV
                     MHRVHDMGLNLVQIGIRAQCIEEAELIKKSKNIHTYYAHDVRSNKNWMKDAVKDLTEN
                     VYLTIDADGFDPSIMPAVGTAEPRGLFWEETLTFLKKVIKTKNVVGFDIVECAPMKGT
                     ILSEYTLAKLAYRLIGYLEIK"
     gene            complement(35456..36667)
                     /gene="sucC"
                     /locus_tag="PK736_00930"
     CDS             complement(35456..36667)
                     /gene="sucC"
                     /locus_tag="PK736_00930"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRC31986.1"
                     /translation="MNLHEYQGKEILKSFGVAIQEGIVADTVEGAIAAAEELTKQTGT
                     KYWVVKSQIHAGGRGKGKIAGTEQRGVQVAKSMDDVRTITKNILGNVLVTIQTGDAGK
                     KVNKVLIAQDVYYPGPNETKEFYMSVLLNRNTGRNIIMYSTEGGMNIEDVAHNTPHLI
                     FKEEVDPSVGLQSFQARNIAFNLGLSGNAFKEMVKFVAALYNCYVQTDSAMFEINPLF
                     KTSDDKIIAVDAKVSLDDNGLFRHPSYEALRDKMEEDPTEVEASENELNYVKLDGNVG
                     CMVNGAGLAMATMDIIKLAGGDPANFLDVGGTANAARVEKAFRIILKDPNVKAILVNI
                     FGGIVRCDRVAQGIVDAYKNIGEIKVPIIIRLQGTNAEEAKKIIDESGLKVYSAIQLK
                     EAADLVAKVLA"
     gene            complement(37143..39161)
                     /locus_tag="PK736_00935"
     CDS             complement(37143..39161)
                     /locus_tag="PK736_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC31987.1"
                     /translation="MKKNKIKTAVPEKKIGKQNTEPKAPSLKKYYFNIFAISLIILLG
                     AVIYSNSFDCSFHVDDIPSITNNNQIQKLSDINAIWNYNHSRFVSYLSFAVNYHFGKE
                     NVWGYHLVNIIIHILNALLVFWLTTLIFTTNALKETATNHHKRIFALVAALLFVSHPL
                     ATQSVTYIVQRLASLVTLFYLLTITLYVQGRVTQNNISRFACFAGALLTAILAMFTKE
                     NAFTLPFAIVLVELFFLQSSKFTFNFSRKHIAIFIVACVGFIFFVANKFSLNVLKPLP
                     PDVYNDFTEISSTNYLFTQFYVLLKYIQLLFLPINQNFDYDIRLSSGFFEIKTIISFV
                     ALVAILVWGIWLFNKNRIISFGIFWFFLTNVIESSIIPISDLIFEHRTYMPSVGFFLA
                     FVAGVFALLKNKNMNIALAIFGVIILFNSALTYQRNIVWKSDFSLWSDIIKKSPNKAR
                     AYVNRGKIYNELRQYENAIADLTKAAEVDPRYALAYTNRGFAYKSLGNWQKGLEDYTK
                     SIEIEAKNSLAYSNRGNIYVNLKQWDKALADYNQSIKIDPAFPLAYLNRAFVYNSLEQ
                     WEKALEDCNVAIKLNPKHGGAYASRGPIYVKVGKIDSAIADYNKAIELNPTTPHSYFN
                     RGSVYLDRKEWEKAYRDFSMVLQLEPKNTGAITARDYAYNQLNAKKSP"
     gene            39741..40673
                     /locus_tag="PK736_00940"
     CDS             39741..40673
                     /locus_tag="PK736_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC31988.1"
                     /translation="MKIAVIIPCYNVAAHIESVVEKIPANIDWIIAVNDCSKDDTANI
                     LLKLAKHSKRIIYLQHEKNQGVGAAMLTGYKKSIELKADISIKMDGDGQMDASYIPAL
                     VNPLVEDKADFTKGNRFHNLRALQRMPITRRIGNTGLSFIIKASSGYWNTFDPTNGFT
                     AIKNETLMEMDLYRIHKRYYFETSMLIELYYRSAVVKDIPMHANYGEEISGLSRTRVF
                     FEFPPKLLYAFIRRLLLKYFLYEFNVASIYLLTGLPMFCYGLAFGIKNYLKYTSIGLP
                     APTGTVIIPTLLIILGFQLLLSGVSYDIGNYPKK"
     gene            40735..41724
                     /locus_tag="PK736_00945"
     CDS             40735..41724
                     /locus_tag="PK736_00945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC31989.1"
                     /translation="MQPNILLSFIVPVYNAENTLAKCIDSILAQPEKVFEIILLNDGS
                     VDKSQLIIDKYVLENPSIVKAFIKENSGPGSARNFGIQNANGIYIAFVDSDDYIEPNY
                     INVVKTHIDEHRADMVIIGYNRMYNRKQSLLERFHKFSNFDLYNQPIEIKTKPQMLCD
                     IEVACWLRIVKRELFLADENLFFSSNSLAEDLEASLKWYINTKVIVVSPIKLYNYIIK
                     TNTLNFNTGFISQYINIIESVCNYYKAKSQFNYYFEELEYMFAKHMLIANLLRLRAAK
                     QEAKFEQFITMRKSLLQYFPQYAKNKYFKNDPIYIRAAIYISYYFPKLFYPIL"
     gene            41724..42584
                     /locus_tag="PK736_00950"
     CDS             41724..42584
                     /locus_tag="PK736_00950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRC31990.1"
                     /translation="MEGRVNLSVIIPMYNAEKYIANTIASIQNQQKHSMVYEIIIVDD
                     CSTDQSRQVVKDLNSDNIRLIELKQNGGTSHARNAGIAAARGEWLQFVDSDDAICSDL
                     YSKFEAAQRPLINCYVFSLIIEEHNQTIQRKIIQVKDKRAIGYFYAVWNFFIRKEICA
                     EFNHISRQNEDVCFIVDMMNTKNLEVDLIKDAYYILNRKNDASKMANFSEKEYLKMYN
                     YVYGRIDHCDVLTKMFILESFVGIVFSKGMPLLMSLRVAMRTIFKLFYYLPAVYSNGI
                     RNCVKNTTIA"
     gene            42611..43486
                     /locus_tag="PK736_00955"
     CDS             42611..43486
                     /locus_tag="PK736_00955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC31991.1"
                     /translation="MQQDHTITKSIDISIIIPMYNAEQYISRAIASVINQQAHNFNYE
                     IIVIDDVSTDGSCKVVKALNNPHIKLIELTHNGGTANARNQGLKIATGRWIQFLDSDD
                     FICNDLYAKFEKKISPGYNCYLFSVIYEYSNYNLKQTITYIDDKRAFGHYGSVCNKFI
                     KRENCLPFKKEFSFEDVCFIFDMMMETELKIALIDDAFYYYNCQNTSSKMANFNAVEF
                     EKMYAYVYSRIDRADALTKMFFLEIFVAVLFDKQRPFFMSLRITIKTLIKLWRYLPNV
                     IRNQNRGYILSASIA"
     gene            complement(43541..44626)
                     /locus_tag="PK736_00960"
     CDS             complement(43541..44626)
                     /locus_tag="PK736_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HRC31992.1"
                     /translation="MNVGNIRAIAFYLPQYHPIPENDIWWGKGFTEWTNVSKAKKYFP
                     GHYQPRIPADLGYYDLRVAETRQAQVDMAKEHDIEGFAYWHYWFGNGKQLLEGPFNEV
                     LKSGEPNFPFCLSWANETWKGFHHGLTNRNVLIEQLYPGAEDIEKHFYEVLPAFLDKR
                     YITVQDKPLFTILQPLLIPDAKAFIEQWQNLAIKNGLKGIYFVGQSNKFEDINKILEA
                     SFDAVNINRLFDFLRLKKSLPSIALNLVKSKIFGVPHVYDFGNASKFFTGKEDSDTNC
                     IPTIISGWDHSPRSGKNGLVLHKATPELFEQHVIDIFQKVKHKPSDERIVFIKSWNEW
                     AEGNYLEPDMKYGTGFLEAFKRAVLKC"
     gene            complement(44633..45802)
                     /locus_tag="PK736_00965"
     CDS             complement(44633..45802)
                     /locus_tag="PK736_00965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC31993.1"
                     /translation="MNILVFAHDAFLYGASQSLLTALKGIRKKADRNILVLLPFGGVY
                     EKELIDAEINYKIIPFPRCVDFNYATASRLKRIQNQYSYYLNRISILKELIKTAKDFE
                     ADVIYTNTSVVSIGYTIAKKINVPHVWHIREFGDLGFGFKYYPNREKIAKLVNKSTKS
                     IFVSEILKKHWCAKNSIRQSVVYNGIVASNKFTANLFKTFPIQNFKIGMIGAVSPGKE
                     QHLAIEAFAELIKVIPNCELYFYGGVNQNYYKSLLKLIGAFQLEAKVIFQPFTKDKNQ
                     IYENLDLVISCTKLEAFGRTIVEAMDYGIPVIANSAGGVLEIIDNNLNGLLYNGTAEG
                     LKNEMVKVLTDKNLYESLSLNGLRKAKFFGVETYVNSIDTILYEASINKNKFENK"
     gene            complement(45803..46861)
                     /locus_tag="PK736_00970"
     CDS             complement(45803..46861)
                     /locus_tag="PK736_00970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC31994.1"
                     /translation="MKVLIVYPEVNPNDNPFTRTLVKGLRDLGCDVNWGRKEFWNNWA
                     HYDIINFHWPDAMFDKMPPSDSDFELLEKHIQVIKRTCKIIYTRHNEMPHYTTDANRH
                     KCYKLIEKNADAIIHLGRNEIELYKERLNNSSVKHFFIPHHIYENMYNNTITIKEARQ
                     KLNIPLDKFVVLTFGEYRDMEEADMVLSEFAKAKIQNKLLFAPRLMKLLIKHTPKNVF
                     LKWLESKTRTMRLKSQKIKTGIEFVSDDVLENYFSAADVVLIQRKHILNSGNVPLAFY
                     FKKPVIGPNVGNIGELLKHTGNALFDPYKKGSLCKAFHEAEKLAATNLGQNNYDYAVK
                     NMSIAVVAQQYLDVYKTV"
     gene            complement(46874..47980)
                     /locus_tag="PK736_00975"
     CDS             complement(46874..47980)
                     /locus_tag="PK736_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HRC31995.1"
                     /translation="MNANTNGIKTIAYYLPQYHPIPENDEWWGKGFTEWTNVAKAKPL
                     FKGHVQPFLPGDLGFYDLRVPEVREAQAQLAKDAGLYGFCYWHYWFGGGKQLLERPIN
                     EVISSGKPDFPFCLAWANESWSGRWHGLHDKVLATQEYLGEHDFEQFFNSCLPAFQDK
                     RYIREGGKLLFSIYQPFGSPLIPEFIKQWHNLAEKNSLGGFHFNAINARANALKLGFD
                     SYTSGTPRSKQANNFFNRLSLKHFNIRISDLIRNFPHQGPAIYNYKDVVAEHLNKPIP
                     DKEIPVILTNWDNTPRSGRAGIVCESASSEQFELYLKKAFAAVSANPNTSQIVFIKSW
                     NEWAEGNVMEPSRLHGNSYLQALKSVTQQHKNNN"
     gene            complement(48259..48369)
                     /locus_tag="PK736_00980"
                     /pseudo
     CDS             complement(48259..48369)
                     /locus_tag="PK736_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015274446.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(48374..50014)
                     /locus_tag="PK736_00985"
     CDS             complement(48374..50014)
                     /locus_tag="PK736_00985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC31996.1"
                     /translation="MRIAICTTNREAYSETFVKAQVDFLPAALLLHYGMPPTMVDGKL
                     IKAENKRIAELTGGTKVAQESLSLEDEIVCLLKREKIDIVLAQFGPVGYSIREVCRKA
                     QIPFVVHFHGYDASGNKLLADYEEKYKLLFSDAKGVIAVSKIMAQKLHKIGCPAEKIT
                     LIYYGPNAKFFDLKPKYDSVTFFGIGRFVDKKAPYLTLLAFAEVLKKVPEAKLILGGD
                     GILENTCKNIARAFGIENSVSFPGILKPEETMQLMENALAYVQHSVIADSGDSEGTPV
                     AVLEASAAALPVIATYHGGIPDVIINNETGFLLEEYDIAGMAQAMLTLIDNKEKAKKL
                     GMAGRERIKNNFTMDKYISDIRNLLNTTVENEKEILIAQLKQIVEEQEVKASALETNV
                     LELKKIIEEKENSVLELKKIIEEKESNASELKKIVTEKENTIEQKENNVIELKKIVDE
                     KENTVKSLENTIERKENNVKELNKVVEEKENKVKTLENVINEKATKVKQLESIIGEKE
                     KIITAKDIRIESIYESITFKLGKVLLSPIVFVVNLFKK"
     gene            complement(50085..50969)
                     /locus_tag="PK736_00990"
     CDS             complement(50085..50969)
                     /locus_tag="PK736_00990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005803734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC31997.1"
                     /translation="MMVEIKNKNTLPPLVSIACITYNHSQFIKQCLDSILMQKINFTF
                     EIVIHDDASTDGTREIIEEYATRYPNIIFPMYQTENQFSKGVRGINPRFNYNRCRGNY
                     IALCDGDDYWTDENKLQKQIDFLQANDEYSMCCHDALVKPYYGKDFIYNDYKSDFDFE
                     FERLIYQNRIPTASIVYRKFYIDPKFFNGIMAGDWLLNLLNAERGKIRYFNSVMSVYR
                     VHTNSIWSGMEGNDIILKGVTVMDKLNRHFKYKYDSIFKDAIRKRMEKYSYSKNKKGY
                     LWNMRKKIIFQIKRKLTF"
     gene            complement(51416..52225)
                     /locus_tag="PK736_00995"
     CDS             complement(51416..52225)
                     /locus_tag="PK736_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HRC31998.1"
                     /translation="MQKIGIHSVPRSGSSWLAQIFNSAPHVVVKFQPLFSYAFKNYLN
                     ENSTCEDISNFFAQISLSDDAFINQSQQRDIGIYPTFKKDKNLTHIIYKEVRYHHIIK
                     NLLMKDKEIILFGLIRNPFAVINSWLNTPREFRKDLGWSEEQEWLFATAKNENKIEEF
                     FGFNKWMEVALMFEELAAIYPERFYITQYSNLLADTHTEIKKMFNFARIPMEQTTVDF
                     IQASRNKNDNNKYSVYKQRAMDENWKNELSPTIILGIEKILATKTQLLKYL"
     gene            complement(52225..52920)
                     /locus_tag="PK736_01000"
     CDS             complement(52225..52920)
                     /locus_tag="PK736_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HRC31999.1"
                     /translation="MKIGIMQPYFFPYIGYFQLIEAVDEFVFYDDVQFINSGWINRNR
                     ILINNEAKMITVPLANASYKKLINEIPLLPKTKYKILTSIELAYKKAPNFETVFPLIS
                     EVIATDYKMISELSTASVMCVSQYLDLKTRFHISSQSFANSAQLKKENRLYDICAQTK
                     ATTYINTIGGKSLYNKQDFANRNIQLYFTEPNSINYKQWGNNFIPWLSIIDVMMFNTK
                     AEIKQMLSNYKIT"
     gene            complement(52925..53998)
                     /locus_tag="PK736_01005"
     CDS             complement(52925..53998)
                     /locus_tag="PK736_01005"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRC32000.1"
                     /translation="MIPITKPFLPPREEYIQYVDDIWKRNWLTNNGPLVKELELKLKE
                     YLSVKHLLFLGNGTIALQIAIKALELKGEIITTPFSYVATTSSIVWEGCTPVFVDVDA
                     TMNINPLLIEAAITKKTSAILATHVYGNPCDIAAIAKLAKKYNLKVIYDAAHCFGSMY
                     KGKSVFNYGDITIGSFHATKIFHTIEGGAVTTKDKALIKKMSHLRNFGHAGPDKFIGV
                     GINGKNSEFHAAMGLTNLKYVDEILAKRKSDHQLYDNHLQHLQLQKPLVLKNAGYNYS
                     YYPVAFKTEKDCKKVFDGLQKYNVFTRRYFYPLLSTLDYVKHKKMPVADNLSKRILCL
                     PLYFELSKAEIDLICKMIIRILK"
     gene            complement(53995..>54904)
                     /locus_tag="PK736_01010"
     CDS             complement(53995..>54904)
                     /locus_tag="PK736_01010"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HRC32001.1"
                     /translation="FNPLLSGRENVYNNGQLLGFSKKEVEERFNAILEFAEIGKFIDS
                     PVQNYSSGMKVRLGFAIAAQMEPDILLIDEVLAVGDMGFVLKCFNKMDVLRSKTAMIF
                     VTHGLSQVSRMCTDISILNEGKSVYQSSNVAAGITRYYEMFKTQIVSFKASDKVSIEK
                     ITLSSEGKESTDEKTLTVEHGAELCLSLELNCIKPVYNPRIALIFYDKEQKNIAETLN
                     FDQQIVLPEVEGLLSIKAVLPQVIFSQGNYSITIALSEIENGSRKIVFRQQSAIYFNV
                     HSKVHGWAPVQLDFNWELVSGNVNTP"
BASE COUNT        16960 a        10072 c         9763 g        18109 t
ORIGIN      
        1 tgatggccga tatggcgaat gattttttgg ataaagaagt tactccctac ctcgaccgta
       61 tagatgcgca agaagaaggc ctcatgcaaa gtatattgga caaggccgga gcactgggtt
      121 tgcttagtgt atcgttgccc gaagaatatg gaggacttgg taaggatttt acaacgggta
      181 tgttgattac cgaagcgctt ggtgcaggtc attcgttttc ggttgctatg gctgcacata
      241 caggtattgg caccttgccc attttatatt atggtaccga tgcacagaag aaaaaatatt
      301 tgcccaagct tgcaacaggt gaatggaaag cctcgtattg ccttaccgaa cccggttcgg
      361 ggagtgatgc actggccgca aaaacaaaag caatattaga aagcgatgga aaaaattatg
      421 tgctcaatgg acaaaaaatg tggattacca atgcaggctt tgccgatttg tttatagtgt
      481 ttgcacaaat tgatggcgat aaatttacag gctttattgt tgagaaaaat gccgttggtt
      541 tatcgctcgg caacgaagaa cataagatgg gaataaaagg ttcgtcaaca cgacaagtgt
      601 tttttagtga ttgtaaagta cctgccgaaa atgttctggg cgaaattggc aaaggtcatc
      661 taatagcatt taatatttta aacattggcc gtgcaaagct tgctgccgcg gcacttggcg
      721 cagcaaaaaa agtttgttct acatctattg cctatgctct tggacgcgag caattcaaaa
      781 tccctattgc aaaatttggt gccatacgtt ataaacttgc cgaacaagcc attcgtgttt
      841 ttgcatgtga aagtgccatg tttcgcatta gcgattatat tgatgaaaaa gaaaaggcat
      901 tgattgccga agggaaaaat ttttcgcagg ctttgcttgg tgcagccgaa gaatatgctg
      961 tggaatgtgc aatgctaaaa gtgtatggca gcgaaacgct ggattatgta gtggacgaag
     1021 gggtacaaat ttttggcggt tatggtttca gtgccgacta tcccatggac cgtgcttacc
     1081 gcgattcgcg catcaaccgg atatttgaag gtaccaacga aattaatcgc ttgcttactg
     1141 tcgatatgtt gttgaagcgc gccatgaaag gccaactcga ccttatgact cctgccatga
     1201 atgtgcaaaa agaattgatg gaaattcctg atatgtctga aggcgattct tcgcagcctt
     1261 tctttgctga gtacaaagca attgcaaatt tcaaaaaagc cacattgatg gttgccggtt
     1321 cggcagtaca aaaatttatg atgcagcttg ctaaggaaca agagatactg atgaatgttg
     1381 ccgatatggc tatcaattta ttcgtggccg aatctacttt gctacgtata cacagactgt
     1441 tacaacttaa aggaaaagat gcggtaactg ttcatacgca aatgttgcag gtgctgatga
     1501 atgacacggc cgatagaata aatattgcag gaaaaaatgc catacaatca tttgccgagg
     1561 gcgatgaaat gcgcatgatg atgcttggcc ttaagcgatt tacaaaagtg aatttggtaa
     1621 acacccgcga tggaagacgt gcagtagcaa aggcattgac cgacaacggc aaatattgtt
     1681 tttaatattt tatgacgctc ccttttcccg attttattat aaagctacaa catgctttaa
     1741 cttttccatt acccggtata gaagcgcata aagaaatggt gccggttggt cgtgtggctt
     1801 atgaacaact tcaaaatcaa attgaagaat atagaaaagg tgcggtgctt attcatttgt
     1861 gttttgataa tgatgaatgg aaagttattt ttatccaacg aagtacttac gatggagtgc
     1921 attcggcaca gatggctttt cccggtggca agtatgacga cagcgatggc acatttcaaa
     1981 atacagcaat gcgcgaagca tacgaagaag tgcaactgca tgcacaccaa ataaatttta
     2041 ttggtgaact atctgtgttg catattcccg tgagccgatt tacggtttat ccttttgtat
     2101 cgtggcaaca tggtatgccc aatttagtgc ccgatgtaaa cgaagtcacc agcattcaca
     2161 tacttcccct tgctgatttt tataatgata acaatcgcat agaaaccgaa gtaacattac
     2221 aacagggtgt aaaaaaaaat gtacaggcat tcaatattca aaacaatatt atatggggcg
     2281 ccacagctat gataatgcat gagtttgtta ccatattaaa aggtttaaag gttcaatagt
     2341 ttagtggctc aatagttcaa gagtttaata gttcaaagtt taatagttca aaagttcaag
     2401 ggtttaagag ttcaatagtt caagtgttta aaagttcaat agttcaaggt ttaagagttc
     2461 aatagttcaa gggtttaata gttcaagggt ttaatagttc aaagtacaag agttcaataa
     2521 gacatgatgg actatgaaat taattcaaac gaagaacgaa gaacaattct ctccgcaaac
     2581 gaaactaaga acattacgta tcgcaaacga aaataagaac gaagaacaag gccctccgca
     2641 aacgaaacta agaacgaaga acaaggctaa cagtaaacgt aacgaagaac aatcctctcc
     2701 gcaaaagaaa taaaaaatga aagcaataat attcgacatg gatggtgtac ttatcgacag
     2761 tgagcctctg tggcgcaaag ccatgattgc aaaattcaat gctgtgggtt tacctttcga
     2821 tgatgatcgt tgtatgcaaa caaccggtct tcgaatagat gaagtaacac aacattggta
     2881 caacttattt ccatggccaa atgtgactcc acaccaactt gcaatggatg taattgatga
     2941 aatgataaga ctcataaaca ttgaaggcga ggcaatgcca ggggtgtatg agactttaaa
     3001 aatgttacat tctgcaaact gtaaaattgg tttggcttca tcgagttcta tggtattgat
     3061 aaaagcagtg atagaaaagt tagaaattaa agagtacttc gatgcgatac attctgccga
     3121 atttgaaaag tatggaaagc ctcatcccga tgtatatata catacagcaa atttattaaa
     3181 cacaccgctc cgaaattgta ttgccatcga agattcgctc aatggattaa tttcggcaaa
     3241 ggctgcactt atgaaagtga ttgccattcc cgaagcccac aaccatcacc atgctaaatt
     3301 tattattgcc gataaaatta ttcattcaat gcaagaactg acaatggaaa taataaatga
     3361 gttgtagttt tattgcggaa aaaatgtgaa tgaattgcga attgtttctg caggctcgca
     3421 atgctgcatc acatgaatga ataccttctt tcttttcatc atttggaaca agataccatt
     3481 aagacaattg caactgaatt tgggtattaa gttattgtga taaaaagtta ttcctcgcag
     3541 aaagtaatat caatgacaaa caaaaccaac gactatccac catcatgata atttccaata
     3601 tctggcagag atgcttcgta actcagcatg tcaacttaag caaccaaagg atcatgacca
     3661 atgtctggca gtgatagctt cgcagtttga gaatactcat tgtgacaatt cgcaatgacc
     3721 ccttcgtctt cgctcagggt gacaatgacc aatgacaatc cccaatgacc aatgacaatc
     3781 cccaatgacc aatgacattc ctgaatggcc aataaccagc agagatgctt cgtacctcag
     3841 catgaaaaat tcagcaaccc aataaccaat caaccaataa ctcaataaca aataacccaa
     3901 caaccaaata accaataact caagaactaa tcttcgccca actatctttt aggcctacag
     3961 ttttattgaa aactaaatgt tgtggagtag aatctttatc aaagcaaaaa taacccaagc
     4021 gtaagaattg aaactttgaa tcaatggaat caagtgcaaa atgcttttcg atgtatgctt
     4081 tatttattat ttttaaagaa tcgggattta catagtgcaa aaattccttt tcctgattgt
     4141 caggattttc aactgtgaat aacctatcat ataaacgtac ctcagctgtt atgttttcgg
     4201 tagcgcttac ccaatgcaaa acacctttaa cactaatgcc cgaagtgtca tgtccactgc
     4261 ggctttcggg tacatacgtg caattgagct gagtaacttt atcggcatca tcctttatta
     4321 cttcatcaca tttaataata aaagcatttt ttaatcgaac ataaccaccg ggtgcaaggc
     4381 gaaaaaattt cttcggtggg ttctccataa aatcttcgtg ctcaataaat atttcttttg
     4441 taaaaatcat ttcacgattt ccagcctcag ggtcttcagg attattttcc gctgatagaa
     4501 tttctttttt tccttcctcg aaattggcaa taacaatttt caatggatcg attacaacca
     4561 ttctgcgtaa agcaatttta tttaaatgct cgcgcacaca aaattcaagt aatccaaaat
     4621 ctataacatt ttcgcgcctg gcaacgccta tggtgtcgca aaaattgcga atggcttcgg
     4681 gcgtaaatcc gcgcctgcgc atagccgaaa tggttggcat acgcgggtcg tcccatccac
     4741 ttacatattg atcctgcaca agccttagca atttgcgttt gctcattacg gtgtaattaa
     4801 gattgagtcg cgcaaattct gtttgttgcg aaggataaat ttccaattgt tttatatacc
     4861 aatcatagag cggacgatgt acctcaaact cgagtgtgca aatggaatgg gtaatttttt
     4921 cgatgctatc actttgtccg tgggcaaaat cgtacatagg atagatgcac catttatcgc
     4981 ctgtgcggtg atggtggtcc ttttttatgc ggtacataag cggatcgcgc aggtgcatat
     5041 tgggcgatgt catatcgata cgggcacgca aagttttttc gccctcggca aattctcctg
     5101 ctcgcatgcg tgtaaacaaa tccaaattct cttccactgt tctgcgtgtg tatttactaa
     5161 gtgtgcccgg ttcggtagga gtgcctttgt gcgctgccat ttgctccgca gtttgatcat
     5221 ccacataagc caatcctttt ttaatgagta tcacagcaaa atcatacaac tgctcaaaat
     5281 aatcggaggt gtataattcc tcactccatt cgaagcccaa ccattttata tcatgcttta
     5341 tagattctac atattctgtt tcctcttttt ctggattggt atcatcaaag cgcaggtttg
     5401 ttttgccgcc atactttagc gcaagtccaa aatttaaaca gatagatttt gcatggccaa
     5461 tgtgcagata tccgttaggc tcaggtggaa agcgggttag caagcgcccg tcatgttttt
     5521 tcgtttttaa atcttcttct acaagttgtt ctataaagtt gagcttttca tgctcttcgt
     5581 ttgctgtatg attcatcaat attttttttg cgaaattaat tttagttgca cacaaatcaa
     5641 atttatagca acaggttatt tgtttttagc caagtctatg ttttcatcac ctttcttata
     5701 aggcaggtaa gtcataatca attgaaacat ttcatcggtg gttttcatat tgccgcctat
     5761 gggttcacgc acagtgcgtg gcggaaaaaa tggattgttg ggatttttgt cggtattatc
     5821 aaacacgcct tcaacataaa tagtggaacc tgcaggaata atcaatggct tagggtaagt
     5881 gtaattatat tgccaacgga aatcccactt aggaattttt actattcgta tactgtcacc
     5941 gttaggagca atggcataag cccaaaacga tttgcccagc aagtgcatgt gggggtttat
     6001 tgtgagcaaa gaaatatcga caggtaccac agcattggtt ttatatgtgc ttatcttacc
     6061 gggtggaata acaagtggtg gcacaattgg cgaaatgccc agggtaccaa gttgaatttc
     6121 gcgggtgggg cgcaagggtt ttttatcggt aaaaaaaata ttgaagtacg atgaatccca
     6181 ttgttcaaca gtggttgcac cataatgaat atcgcgcagc aacaaagcat tccgcttatg
     6241 ggaagtatat ccaccaatat tttcgggata aataattccg ctgctgccgg gtaaataatt
     6301 tgccacgctg ccaataaata taggggtggt gccatcatca tacaataaat ttaagttatg
     6361 agccgattca ctaatttcgt aagctgtatc gcgatcgata ataaatggtt ggcggtataa
     6421 atctttcgag cttttattgt tatcgaaaac aatcatatgg ccattcatgt ggtgcaacaa
     6481 ttttttatta cccggtacaa attcaatact acgtataaag gtatcgttag gcaactcaaa
     6541 gggcactttt accattataa acttttcgcg gttatcgcct tttattttaa atggtgggca
     6601 ggcaatcacc atatcgggat ttgccagttc gctgcctttg ggaaaatcgg gaactggcgg
     6661 tatggctgcc gaatcgccca tgggtgcacc accttcggcc caatctgcaa taatttttat
     6721 ctcctcttcg gtaagatatt tttcatcaat aaagtgcaca taattatggt cggcaggcca
     6781 tggaggcata aaccgcgact gtgtggtata taaaatggta tgtgcatatt ttttacagtc
     6841 ggaataggtg aggagtgcca tggtacccgc ttgccccggg cgatgacaac cgctgcaatt
     6901 tttataaatg ataggcgcca cctccacaaa agttggtgta cgtttaccac aagcggctaa
     6961 agcaattgct aaagcaattg actgataaac caaaaattta ttcatcgtga agcgccaata
     7021 catttttgca ataatatact ttattcgata aagcatccaa tgggttttgt tttttttaca
     7081 gctatttctt tttgttgcaa tacggcctca atagcatcat gcaaaaaatg ttgggtcacc
     7141 acttgccgtt tcactccaac atcggcagca taattatcta tcagcccttc gtatataatt
     7201 tccattttat tgtttatcaa aaagcaatgt ggagttgtga gcgcgtggaa tttattggtt
     7261 gctaattttt ggttatcaat aaacaaatcg aaatttattt tgtaaaaagc ctgatagtat
     7321 tttatagtgt cactgctaaa tccatcgccc ggaacaagac caataaattt aattccactc
     7381 ttcgagtatt ctaaatttaa attattgagt gtagtagaat acccctgaca aatgggacac
     7441 tcgggcgata aaaaaacaag aacagtgtaa ttggaagaga gttggttttg gagggttgat
     7501 gaagtgtgat tcaaattaga aatctccagg tttgatgttt tattgcagct gcagcaagcc
     7561 aaagaaattg acagacacaa tgcgattaaa aagggaaata tttgtacagc aaaaattttc
     7621 attttatctt ccacttgata ttttgttaat gaagattttc aatatgtatc ttaaccatat
     7681 caatttcaaa tataagtaga aaaatcaata aggcgtgttt tatcgtatac attttgggtg
     7741 aaagctattt attcaaaacc gatacattga cattatcact attttcgcaa actattaaat
     7801 ccggactatt ttttctaccg gacttttaat aattgatatt ttttagttca atttttttaa
     7861 tttattttaa atgttcaagc ttatttttag aagactttta gcattgatta ttttttcaag
     7921 catctctttg ccttttttca gccaaaattt gcagggaact cttggttccc aaataactat
     7981 acctccaaac tattttggtt tcaatggtgc taatgttgta agggccggaa acccttcata
     8041 caatactccg tggcttatag atagtatagg tttgtttggc cctgaaatta taagatatcc
     8101 tgccggagtg ccaagtggat tttgggattg gcaaaaaggt actttatatc aaaatcctcc
     8161 cggtggattt ctgctgccaa gggaatatga tggttatatt tttaaaacta atactttgga
     8221 catgtataag tccatggtag atttgcttgg ccttactcct ttacaaaaca taaacgtatt
     8281 atgcagcgac ccatattatg aaaccgcaag attatattat tcgcggggta taaatttacc
     8341 ggttaaatat attgagtttg gtaatgaact gtacgatgac gaatacccgc agtatttgca
     8401 aaagtggcct actgcaaacg cttacattga cgatatgaat aattttgcat cagtgttacg
     8461 tagtttcccg ggcagtaatg aatttaaaat tgcggctgtt ggtgctgtca atcgtttagg
     8521 attggaacct gcagaaagca gacgcaacac atggacatca ataataacat cgaatgccag
     8581 tgccgatatt gatgctataa ccttacattg ttacatcggt acagggcttg atgaaaatgt
     8641 tgctttatcc ttgagtaatt taccacaagt attatctaca cctttcaagc gtatggatga
     8701 tttgtcgaat attgaattag cgcaactgcg taacgcagga aaagaagcct ggataacgga
     8761 gtataacgtt ttcgacaaaa aacactgtat acacagcact tgggcacagg ggcttatggc
     8821 agcaagtatg actctatcat ttctaaacga ttcaactatc aagcttatca atttccattc
     8881 catggtagct gatggtgtat ggggcagtat ttttaaagat tcttctgatc ttgatataag
     8941 tacattttat aatggtgtta catgtaccac aaatcctcct caagcaaaac cgcaacagcg
     9001 ttcaggttgc ggtaacgcta tgttgctggt aggtcaggca cttaaaggaa gtgcttcggt
     9061 tcgcaaaatc tcattgacaa ataatcctgt tttatatgat gtttataatt ctgtacatgg
     9121 atggcaattt gacggaccaa tagcaacaca gggaatattt ttgaatttat ccggcactat
     9181 tagaacggtt gtaataaacc aggcagattg ggacttatca ggctataaat ttgaacaaat
     9241 aagtgtaggg cccggaggac ctttggcgtt aataactggt aatgcaacta agacaccggg
     9301 tgtaaatgat gttgagactt cggggttcaa taactttgtt ggacaaacaa ttgttttaaa
     9361 accgtattca gttttcagat tggtaaaacc taagaccgga ctcttaatga aggttgtgga
     9421 taataccatt tgtagtggca ccactacatc attggttgca caaggcacag gtaattttag
     9481 ctggacaccc atggcaaacc ttatttcgtt gaaacctgat aacagtgtta tgcaatttac
     9541 cgctacagta actaatacca cagtttatac tgtaacagta actgctcaga atggaattac
     9601 atcatctgta actataactg tataccctaa gccaaaattg aatgccacag caacattatc
     9661 caatacttgc aaaaaccttc cggtaaaact tgccgccaca tttaccggtg gcaacacatc
     9721 gaactttaat tcttttatct ggcttccttc gacagaagtg gataatccgt tttcggatgt
     9781 agtgaatgtg aacccgaaaa aaacaacaac atacaccgta tacgctaccg atgggcaatg
     9841 ctggtcatcg gttgatagtg tgaaagtgaa tgtttctaat aatgccgatg caactgatag
     9901 caggctggtt tgtcgcgata gccttccact aaaattgagt actaaagtga ataaatacaa
     9961 atacgtttac aactggtata agaacggggt tctgttgggt acgggtaaaa atatttttgt
    10021 atcgcccgat acaattacta cttacatgat ggtaatgcaa agtacaatag attcttattg
    10081 tgtggataca gattttgtaa caataactcc aattacttgc tgtggaagta aggatgcatc
    10141 tatgcttaca tttggtccca ttattaagat gagtgagatt gcagtaagaa taaaaaaata
    10201 ttgcgactct accgcaaacg gtgtcgccac tttgtatggt gtaaatggcc tcgcaaaaga
    10261 aatagttatt aatgggcaat tgattgtcga cagcgattat acatttacca attgcagtaa
    10321 aataaaattt ggcgaaaatg ctgatgtatt atttccgggc gaaggtgcac atttgaattt
    10381 tcagaattgt aaaatttctg cagcagaatg ctccggtcga atgtggaatg gtttcagttt
    10441 ttcggattgg acggaatcgc tcactttaaa taatacaact atttctgatg ccaagattgc
    10501 tataaaggca accctcaatg cgcaacttgt aattacaaat tgtgtattca acaataatta
    10561 tattggattg gatatagcaa caatggagtc tacattcaac cctgttatct atggcgattc
    10621 gttcatttat tctgcactgt tgcctcctta tgaaaatcaa aaaccatttg cgggcataaa
    10681 acttaacgac atcattgata ttactatagg taaagcggga tatccttcta atgttttttt
    10741 gaatagtagc tacggtattt atgccgaaaa tgcacatgtg tatttataca acaatatttt
    10801 tagaaacatt ctgaacacaa ataatggcga tgtgaatgaa ggttctgccg tatatgctaa
    10861 taacctcaat ggatacggag atgttacaaa tccaaaccat atttgggagg agttgtcggt
    10921 tgtggcaggt agcacaacag ctgccaatac ttttgataat tgcttttatg gtattaatgc
    10981 cataaatcat aaacttaaag taaactataa caacttcaat gatgtaaata atgctatttc
    11041 ttgccttggt tgtgtaaaaa aagaaatttc aattacctac aactttatgg atagtgtagg
    11101 cagaggaatt aacctgattg aacccaaact ttcaactatt gatatactta ataattccat
    11161 tttaacatcc acactaaaaa cgccaatagc caatgtcgca gcaattaatg taagcgataa
    11221 tgtaatttac cctagcgctc ttgatatttg gaataatgta gtgacaaact atggcaagta
    11281 tggcatactg gttaccaata atttcaagcc atctattaaa gaaaataaag tatacatgag
    11341 aatgggtact acaactgaga gtgtatacgc actcaatgta ttgaatagcg actcatcgaa
    11401 gataaactgc aactttgttg gaagtaccaa tacaacttta agtaaacgta taggattatc
    11461 ggcatcgttt tgtgccggca gcaattttag atgcaatgtt atagataagt gttataccgg
    11521 aatggagttt tggggcaatt gcaataattc tactattaag aataatgagc ttcggcattt
    11581 ggcggttggt ttagctgttg gtaaaagcgg caccacatca ggtataatag gcccacagcc
    11641 ggtgaatgca acaggtaaac ccacacacaa tttatttaca ggccagtata cacgcaccag
    11701 cggtgccgga aataaaaagg gatatttttc agttgcagct acattggcaa ttaattcaat
    11761 tcttgccgga ggaaaatttg atcttacacg ctatcttgta ttgaacggct taacatcaac
    11821 caacgattcg gtgcaagtgc cttatccaaa ttctaagaca ggtacgggta ctgcgatgac
    11881 acctttttat acttttaaca ctcctgtgac atgtgatgtg aattgcacca tgcaaacagc
    11941 aaaattaaat atgaataatg ctgatgataa tagcgatgat gattttgttg atgtaaaaga
    12001 attgcttcga aataatatgg atgctttggc ttttgaaaaa gagccggtga ttcaatattc
    12061 gttgaagaaa aatatttatg ccttgcaaaa caatgaaggc aataaatata ctgttgctgc
    12121 acagttacat gatattacaa gcatgatgca aaaattgtct tcggtaaata atgatgatgt
    12181 tgagaaaatt gtgattgcaa attcttcgat aaacacgacc gaaaaattcg aagtgaatga
    12241 tcaggcattg aatgatttgg aattgaaata tatgatgaaa aagcaaaagt gttttacaaa
    12301 acctcaaatt gattttattt tttctattgc atcgcaatgc cctgttaatg gcggaacagc
    12361 agtttatcgt gccagggcaa tgtaccaaac aatgtatcca acacatttat tcgatgatga
    12421 aaatgcttgc aacagaatta ccgaaaataa ttcaacagca ttagataata ctagcttgaa
    12481 tgtagaagta atgcctaatc caacaaaggg tttaatgcat atcatgttga gcaacgaaaa
    12541 cgaaacagaa ttgcagatgc taattgccga tatggaagga cgcattgttt tcaattctaa
    12601 tattgctgca catagcattc aaaaaacaat tgatttaagt gcccttgctc cacaagtata
    12661 catgatgaat atttatgaca accaaaacaa tgttatgtgt cgcaaaaaaa tatcagtagt
    12721 gaaattttaa acggaagcat tttttcgtta aaacgaaaaa gcactcacaa caaatagatg
    12781 tgagtgcttt ttttatagat tttattttaa agaaaaaata aaatgccgcc ttcgaatatt
    12841 attgaataac cagccgcact gttttcactt catcggcagt tatgagtgtg atggcataaa
    12901 ttccttttga taacgttgca caatcgagcg aataattttc acttattata tttttaaaag
    12961 aatgttgatt tattattttg ccctgcaaat ctgtaatgcg caactcggca tctttacttt
    13021 ttgcaaagtc gattaatatg tcggctttat cacttgccgg atttggaaat acatttaact
    13081 gagcagttga agatgaattg ttttcactct cgcccaacaa taaatttatg ccaaactgct
    13141 gataaatttc ggcaccaaaa tcgggattga aaacttttat caaagtgcca tcaatttttt
    13201 tgatgcggca gtaacctgtt ccctgtgcag tgttggccca ccacgatagt ccgtcattcc
    13261 caacatctgt taattgaaat tcataacatc caagtggcaa tattaatgta tcgcgataga
    13321 tggtattgtt tgacaatcct agtctgttca caagtacagt accagcatca tttttcaatt
    13381 tgtactttgt accgcttccc tgattatttg tttttacttc aacaataatt cccggtggat
    13441 ataataccgg caaaacatag ttggaacgaa cagtatcatt ttgtgcatac tgatcggtag
    13501 ttgcatttgg attgctaaca taggctataa acgtacctgc ggtgttactc cagttggcaa
    13561 tattcaaagg tacaaatgcg gtatccataa atggcaaggt gccattccat gtaaacgttg
    13621 ttagcgcgct gccttccatg ccataatgaa tatcgagcga tgtgatggtg gtgctgcccg
    13681 tattttttat ttgaacaatt gggttcatgc atacaggatt gccacgtcca tgttctatct
    13741 tatcgtttgg ttttacaata tcaattaccg aggcatcaac tgtgaagctg gcagcgccat
    13801 attctactat ttgattattt acaatgtaat tggatgcacc ggttactaca tttatgccat
    13861 aatcaaaagt tgcatcctga ccgggtgtta ctagtggagt aatatcaaat tcttttacat
    13921 cggtggccat gcccgggcac caacctgcgc ggtcgtaaac ccacgttcct ccctgaggat
    13981 acacagggtt caatgcacat tcttttttta caagccattg aaaatctttt aaaccactat
    14041 caacattcat gtaatgtgtt cgataagtaa attcaccatc ctgaccatgt ccagtaatcg
    14101 cacttttcag tttgaaataa gaacctgcag cattcattgt tactgtgcgg ggttcaaagc
    14161 gatagtcatt caaaatgtta gcatagttat catatgttac aggccacaac tgtcgaatac
    14221 ttaacacagg tgcaggcggt gtacctttta taaaaataaa ttttatgtcc atttcttcct
    14281 gccattcgcc accgcgctct atcgaaatat atttgttatt ttttagcaca ggcgcaaaat
    14341 cggtgacatc aaattcatac atctttcctg ttaaccctaa atcaagattg tttccataag
    14401 gtgtaacaaa ggacataatt tcgaaacgcg aaggccactt ggcataataa tcaagtatcg
    14461 aaattgttac agtactgtcg gctgcataca ttacactatc gacaatagca cctgcctcat
    14521 cgtacacgta gccataacct gcctgccatg cctgtaagga gtatatggta tcgaggtttg
    14581 taccgttgac tgtatacgaa gttactgtgt gagcattgtt tagtaatgaa tcggtgatga
    14641 taatatcttg caaagtgtta gtgtatgttc cctgcgcaaa atttatgttt ggacgtgtgg
    14701 taacaggaac aagatttttg aaaattttat tgccatcaaa tttatcccaa ccaattccat
    14761 ttcgtaaata gcctgttcta ttgtaagttg aaacatctgt gagtacggta tccaaacctt
    14821 cgttcatttc gtaattagcc agcaacgaac tataaaatgg atgtgaggtg tcaatttttt
    14881 tgtacatcca tccggcaatt gctgcagagt cgagggcttc gttccatatg tgcacatcgt
    14941 ctatttgccc atcaataccc gaaaggaatt tttgtacctg cgtgctttgt gctgttttgt
    15001 ttggaatact gccactatat gttaacgcca cctcagcacc attaacataa aacctaagtc
    15061 gcttgccatt gctaactttg gtgccatcat aaaccatagt taggtgagtc catgtattta
    15121 atggcaacac tccacttgcg taaccgtagc tattgttggg gttgcgtact atgcagtata
    15181 cacggtcgag tacatcgcca agttccatag tagttttgcc atctttatta aaaataccgg
    15241 cccagttttt ccatttattt atttttaccc aggcttgtaa agtaaatttc tgcaccgagt
    15301 ctaattcatt aatgtcacct gcatcgccat aagaactacc atttaatttt acattgtaat
    15361 catcggtcat ggaagtgact gcggcatcaa ctgtacttgc ggcatttgcg gtgtgccctg
    15421 aatttgcact tttgccattg taatttattt caacaataat gtttgatgtg ccattccagt
    15481 tgaaaggact gtaaaattga aaacgattgt aacctacgtt tgataatgga gtatttagaa
    15541 aataaacctg attgaaaccc ggagtgttat caatttgagt aagttgagat aatgaagtgg
    15601 ctttaatttt tatacgcaaa aaatcagttt gcgcaccaag ctgtggtata tcgagttcca
    15661 taccactgat atcgcctgct actacaccgg ctgcaatcaa ctcggctgct gtataaaggt
    15721 attgtgtttt tcctgatgta acaccatttg caagtgggtg atttaccagc gttgcaccat
    15781 tacccaccga agccagattt tcgttgataa cactatacac catgtttttt tgaatgtact
    15841 gatagtatgt atacacagga gtgtatgtgt aactaaatgt ggtaccattg aaattggtga
    15901 tatcgtaact attgtgtttt gacttcaccg aatcggtgag tgacgaatcg gtaatgtatg
    15961 tattgcaact gtagtcccat tcaccgcagc catacaacgc attattcgaa cttgatactt
    16021 gccctttctt acaacgaatg cgatattgca ttaatatttt ttgccatgta gaagtgtcgg
    16081 gagggaaagc aaaagtgcct ttgcgcacgg tgctgttaaa gtcgaacgtc tgcactacta
    16141 cagagtcctg tgctattatt ttgtttgtcg aaataatagt acaagccagc agggcaatca
    16201 aacatagttt catttttatc atcttaatta ttgtttttgg gaataaggca aaactaacaa
    16261 aagatttatt atttgcttaa aaatagtttt tggaaatcta ccatttgttt tttctcatag
    16321 ataacgctga tgagcatgga aaaatatcta caacatctgt gaaaatcctt gacatgtgtg
    16381 aggaagcatt tcgcaggcaa aaaaaaatcc aggcactgct gcacctggac taatttcata
    16441 aaattttctt ttcgaaacta aaaacgctcg aaacggttga agaaaaatcc tgcttcaatt
    16501 gcagcatttt catcactatc gctgccatga actgcattgg cttcaataga ttttgcaaat
    16561 aaattgcgga tagttcccgg ttcagctttt gcaggatctg tagcacctat tactttgcgg
    16621 aaatcttcaa ctgcattgtc ctttgttaat attacagcca ctatggggcc cgatgacatg
    16681 tattttacaa ggtcgttgta aaacgggcgc gctttatgca cttcataaaa ctggcctgca
    16741 cgctcaggtg tgagttgcaa atatttcatg gctactatgc caaatccgga tttaataata
    16801 tggtccaaaa ttgctccggt atgaccattg cccactgcat cgggctttaa cattgtgaat
    16861 gtgatattac tcattgtgct tatttttttt tgcgaccgcg aaagtactat ttcatttttc
    16921 gaaaacaact atcgttctgt ttttagtttt tactttgcca attattttca attgcaaaaa
    16981 taaatcactg cacataatta atttaagtat gcacaaaatt acagtccatg gccatcgcgg
    17041 ggcacgaggt tatttgcccg aaaacactat agaaagtttt ttactcgcca tcgacatggg
    17101 tgctaccgct atagaaatgg atgtggtctt aagtgccgat aatagagtag tggtttcgca
    17161 cgagccatgg atgcaccatg ccatatgtac taaacccgat ggaacttttg tgggtttgga
    17221 agaggaaaaa agcctcaacc tttttaaaat gaactataat caaattgctg agttcgattg
    17281 tggcaaaaga ggcaatgtta aatttccgca gcaaaaacat ttacctgcac agaaaccgtt
    17341 acttgacgat gtaattcttg ctgttgaaaa ttacactcgc ttaaacaaac tcaaccccat
    17401 tatttacaat atcgaaacaa agtgtgccag gagtaccgaa ggaatttttc atcctgctta
    17461 tgacatattt acacaattgg tttacgattg ctgtgcaaag caaaatgtta ttgaccgttg
    17521 tatatttcaa tcgttcgatg aacgcaatct aatatattta aaaaaccttg ataagcgtat
    17581 acaactatcg ttgctttccg aatatgaaat aaatttggaa cttgcaattg agtcgatggg
    17641 atttactcct cattatttta gtgtcctgca cactttattg aacgaagagc tagttgcctt
    17701 aattaaaaaa cttggcatgg aaattttatg ctggacagta aacagcaccg aggatatacg
    17761 aaggatgaag caacttggtg tggatggcat catcagcgat tatcccgatc gcgtaatcaa
    17821 tattctcgct gaagattaat ttacttttga cgccaattca ctctagaaat atataataac
    17881 aaaatcaaag acattatttt ttcaatatca tttccacgcc aatttgagtt ttattggtat
    17941 cgaatatttt caataaatat attccaccag ctaaattatt tgtcgaaata atattttttg
    18001 tagaagacga taattgaaca cttactaata ccttacctaa caaatcattt atttctgcat
    18061 ttacagcttt agtgtttttt acatttattt cgatgtagtt ggtaaatgga tttggaaata
    18121 cctcatacat ttcgttttcg tatatttcgc tgatgccaac attattaact gcataaggaa
    18181 ttgatattga agtgcagcca tttaagtcgg taattagcac actataattt ccatttgctg
    18241 taaatacata atagctcaat gttgcgttag gaataatgtt gccattgaca taccattgat
    18301 aagatatggc atttacgttt gaaaataggg tgtctaagtt ttttgatacc ggtgccggtg
    18361 gagtaggtgg gtatagatat acggaggttt cgagcgaatc gcccgggcca cattgattga
    18421 ctccggcagc agaaatttta cctacaatat tattaaattt tacccacacc aatgtgtcgc
    18481 cttgattgta caatgttgct gctggcggca ctttccagta atacgaattt gcacccggca
    18541 tagtatttat ataaaatgaa acagaatctt ttccgcaaat agaatcaaca ccaaatattt
    18601 gtgcaggtgc agttaaaaaa ggcaagcacg taggattgta acgcaacata tttcccagat
    18661 tgcccgcaac ataacccgta tcggctgtgg gaaaatgcat tcctaaattt ccatttgttt
    18721 gtgtgccaac atttacccat gtaactccgg catcctttgt ttcgaaaata gcgcctacat
    18781 cgccaaccat aaatccgtga gtggagttta caaaaaatcc cgaacgaata ttggaaccgg
    18841 tatttcctgt atattgattg gtccaggtgg tgccgccatc gattgattgc actatcacct
    18901 gtgagccacc tccggcataa attgtatcgg cattggcaac tacaagtccc agtaatcctt
    18961 gtgggtcggt gtataccgat gtccatgtgg caccaccatc ggtagttttt ataatgaatc
    19021 cgtttgtaaa agtaccattg ctcgaaactg caaatccggt atcaacattt gcaaaacgca
    19081 tgttgattaa atcttgagta acgccacttg taagcgtggt ccatgtggca ccgccatcta
    19141 ttgtttttat cattacacca ccgccacctg cagcatagcc aataagcggt gtaggaaaag
    19201 tgatagtgcg caaaagacta ttcactccgg tcgataccga atcccagaca gtgccaccat
    19261 cagtagtttt caatattcta ccatttgccc cggaagcaaa acctgtggtg tcatctgtaa
    19321 aacacactgc ccggagcgtg tcggtaaatg ctgtaataat tggagtccat atgttaccag
    19381 catcatctgt gcgaatgatg gtgcttggca atccgatacc ggtaggtgca cccactgcca
    19441 caccatgatt tgagtttgtg aaataaacac cgcgcaactc ggcagtaaca ttgctcactt
    19501 gtggcgtcca tacaaattgt gcattcaaac tacaagcgta tatactcata gttaccatta
    19561 ggataagtct gatttttttc atatcgaatt tgtttaagga gttagagtaa agaattgtgc
    19621 ttcaaatata ataatattgt tggttgataa atggattgaa agaaaaaata ttttctaaat
    19681 ttctgacgta tggcagtttc atttgttttc gaaaacgcta atgccaataa taaattattt
    19741 gatccaattc cgttgcgaaa cttgtttggt tattattata ggcaagtttt cggagaccgc
    19801 ccatgatggc ttgtttataa tcagtagaac tactgcttgt tgattttact tcatcaaatc
    19861 tgccattgat ggcgctttgc aaaagtagcc ttgaaatttc aactgctttt gttgtatcat
    19921 gtaatgcata taagcactct attaaattta aggtaataaa atcaaatggt tcgtaattat
    19981 cgggcatagt ttcgaaacac atattcaaaa cttgtctggc agagtcgagc ttgttttcct
    20041 ttatgagctc atatgctaaa cggtaaaaag taagacgaag ggttttacaa ataagtgttt
    20101 tcgattttga ggttgtgttc atgccggccc attcaaaatt atttatcaca atattgaaga
    20161 gtgtattggt atttatgcca cctgtataat agtcagaatt ttcaaatttc tgtgtcgaca
    20221 atttataaac gatcccttca agagataaat aattctttag ccccaggaaa ttttctccgc
    20281 caatggtgcc ggcaaaataa ataggccttt tgcccatgtg ggagtgaatt atatccaata
    20341 ccaaaatgtc ggaaagtgag agataagatt ttgaatattt ccagtccaac aaaaatgcat
    20401 ctgtgtgaaa agtgaaattg cggctgctta tcttttttat tttttccgtt gttgcctggt
    20461 cattattaac ttcatgtaat tttttcaata catcgctcaa cgaaatttcg gactcttcgt
    20521 ttttagcgaa aagtgcatat tctaaatttt tgttttcaag ttcttgttcg gtaaacgaaa
    20581 atgaaagcgg cactgcacct gcatacggat gcctcatgta atttatgtat cgttcagtgt
    20641 tgagcaaact caaattcaca accaatacat ccgtgcgaaa gccatacatt gcttgcacat
    20701 agagtagagg gtaggtgtcg ttatcaccat ttgtaaaaag aattgcattg ggttcgcacg
    20761 agtttaaata attttttgcc gcgtttatat agaagggtgt atataaaccg ggcttcaatt
    20821 ctttgcgcgc ctcttcttcg ttttgataaa tttttaaatc gagaaacgaa accaaattct
    20881 cgttcgaata ttttattgcc atttcaccta ctatggtttc gaattgagga tttctttcga
    20941 acaatttttt ataaatattg attgctgcat gtctatattc acggtaagtt tctaactgtt
    21001 gtggcgacat acaatttcgg gtattgggca acacaaactg atttttcttt aatgcataac
    21061 ccgtattatt gttgagtaaa ttacttgcat agcttgaata ggctcgccct aatccgaata
    21121 aaaccgaatc cgataaattg ttactatttt taaaatcttc aataacatcc caatcccaac
    21181 caatatatcc ataatatgga caaatgttga agttgtgtat tttccaattg tcatcaaaag
    21241 catagcggtt tgttttattt tcttttgtgt ctgctgcaga ttcaaaattt atttttaaaa
    21301 atgatgccga ttcagtagac caaaaaagtt catctttaaa aacattatta ttcatggcat
    21361 cgcaaaccac cacatgccaa ccatcggatt tcttttcgaa ttttaattca tcggtaatgc
    21421 catatcgccc gggcaaatcc ttcacagaat atttttcgaa aaagttatta agtacatttt
    21481 cgtaagaggg gtacgagatt ttattttgtt gcccatgaca tatcatgttc atgaataaca
    21541 aaaatatgat tacaagtttc attttcagaa tcatctgtgt tttgttttaa aataaaatat
    21601 cgaaagtaat ttcacgcaat aaaattcgga aacataatta cacacattac cttttctcat
    21661 tcgtgcaaat tgttacatct attagatttg tgcacgccaa tcttaagcac accaaacata
    21721 attaaaagta ttggattttt gtgctgctgt tgtttagcgt aagattatgc attggcgagc
    21781 aactaacaag tttagtattt atcgaaattt attttctttg cgtcaaaaaa tagtttttat
    21841 gaataatcgt ttggtgctga ttttattctt actaatatct acagtaagtt attcacaagt
    21901 ttcgcttcaa tggcaatttt atggcgacac tcccgaagaa agccatttca ttgttgccga
    21961 ttcgcacgaa aatgtataca cccttggggc ctatggtggt ttgaggaaat ataatcatac
    22021 gggcgataca gttttttata acaccgatac aacacagtat gaagccaagg ccatagatat
    22081 tgatggcaat gataatattg taactgtagg aacttatttt ggaacaacgc aggattacag
    22141 aattttagtt caaaaatttg atacgagtgg aaataaaatt tgggctaccg ctatcaacac
    22201 accaaatgga aacagagaat ttgcatcagc gttcacattc gatcagatta acaatattta
    22261 tctcacaggt tgggatggcg atcagttttt tggaaaattt tttacggcaa aagtttcatc
    22321 ggccggggct gttgtttgga caaacagttt tcccaaaaat ggtttcgatc catactacgg
    22381 attagatata gccgtgcatg ataccaatag tgtatacgca acaggttttg tagatgaaaa
    22441 tggtacaacc aacgatatgc tcctggtaaa atataacagt aatggcgata ccgtatggtc
    22501 gcgaaaattt ggatactatc aaacttttgg cgggcaatgg actaccgaag ataacggtac
    22561 cgaagtagag acagatcagc ttggtaatgt gtatgtgatt tcgaaagaca tatttggtaa
    22621 tcacgatgtt ttaattaagt ataacccatc aggagcaaag cagtgggcca aagaggtttc
    22681 attatcgcca acccaaatat catttatgtc aatacagaac aataatattt atatggccgg
    22741 taatgccgac atcggttttg gtttgacagc ctcccttgct ataaaaatgg atttgaatgg
    22801 tacagtaatt tttgacagca catttgcaac caagactgcg caaattgcag atgcaaaagt
    22861 atacaacggt ggaatagtta tgaccggaac ttttactcct tgcaatggat gtaactccga
    22921 tattgttaca atgtactttg acagtgtggg caatagctca tggagtaatg tgatgaatac
    22981 atctaacggt ttttttactg accaggctta cggattaact acagataata atggtgggat
    23041 ttatattacc ggcaatggca atggttcttc atactggatg gacacttata agctttttcc
    23101 ctgcaaagat attaaacctg ccatagctaa aaatggaaat cttaatactg caaattatat
    23161 tgcaggcagt acatatacct ggcgcgattg cgcaaacgat tctatagtag cacaaggatt
    23221 tgaattgttt cagtttgtgc aaccttaccc cggaaatttt gctgtggaaa tttctgttgg
    23281 cacttgctcc gatacaacaa gctgcattgt tgcaaacttg aataccattc caaaaaatga
    23341 aaatgaatac atcatttatc caaaccctgc tgtagatatt tttaatgtga ttggaatatc
    23401 aaataataat accaccgcaa ctttatattc tgctgacatg cgagagattg atacatttac
    23461 tttgactccg gaatctgctg ctattgattt aagaaatctt tgtaaaggaa tttatttcct
    23521 ttctatcacc aattcgtttt cgaatagaat tatcaaattg attaaggaat aaaactacac
    23581 tgagtaattc cagagcagaa tgtaaaatgc aattaaatat tatctataaa aaactgccac
    23641 aagtgtgcag tggttattct aatttttgat ttgttactgt gactatatac gttgttttac
    23701 tttttttgtg tcacaagtga agatgcttgg tggaagcgag ggctgtgaga tgacaaatca
    23761 atttgccaat cagagattgg ctaacacctt gacgtagtct ggagactacg ctaaacggga
    23821 agatttttat accactttaa aaaactgcca ccactgttgc agtccatatt ttagtattta
    23881 tttttttgca acttaggtta cagcctacaa actcccacca tcgggggtag gagtatcgct
    23941 tgcaggattg gggcgcatat attctggcct aaccaacgga tatttgttta aaatttctac
    24001 agcgatattt ctaaaatgag ttttgctaac ttttcctatt gcaggagttt cttttagctg
    24061 atgccaaaaa tatccgcccc attcctcggt atcatgttgg catattggtt cattttcata
    24121 gtaaaatggc catagttttt ttgaatgaat cctacttaaa ataattgcta aattttccat
    24181 aggattatat gagtttgttg tatttaagat cacaaaggta gccatatcag caaaaaactt
    24241 tttgtcaatt tcttctttat tgtctgattt ttcaattaat tcaacccata acaaatcaga
    24301 atcttctgtg ttatttattg aactgcattt atagcaatgt actgcaaata atattttagc
    24361 tatttcttcc atctcttttg ctttaatgat taattacaac ccgattggag tagtttccga
    24421 ctacgtcatc gtgtttgcca atcaccaatt ggcaactaca acacttatct tcaaaattta
    24481 gatgtatgcc catcaatttg ttttctcgaa agtacaaatt ctttttaaat gctgtgatta
    24541 ttttatggca ctattttttt tacttcaaac attttcggtt tgtcgttttt atattctttt
    24601 cctgaaattt ctaaactaac actattcatt ttaaattgac agaaactaaa taaatatttt
    24661 agagtgaaag taataaagat tggtatgcga tttcgcaaca aaataagtat gtcgaattat
    24721 ccgaaataat tctacttatg taaacaaaat aatttgattt gctccttgaa aaaaaatgga
    24781 tggctaaata tattattgta aaataaaaat attatttaca tttgcttagg aattattaaa
    24841 aacgattttt ttaaaaattt tggttatgac tataaatgct caaagaaaaa atatcattga
    24901 tcaactgcaa cttattaatg ataaagattt actatctaca atacaaactg taatagaata
    24961 tggagtcaaa aaatcgaact tccaggttat atcaatggaa gaacaaacac ggatattaaa
    25021 aataaaatcg agtaatcggg aggaagattt tattgatgag gaagttttag ataaacttct
    25081 tgattcttaa taatgcagaa atcttacaca gtaaaattat ctcaagactc gattgacagc
    25141 cttgtcacgg ccaaaaatta ttacaataac tgtaagccta accttggaaa aaaatttatt
    25201 gctaattata agacagcgat aaaaaaattg aaaaggaacc ctttttacaa gattcaatac
    25261 aatgatattc gctgcataat gatacccaaa tttcaagata ggattcacta ttttgttaat
    25321 gaagaaaagc aaattatttt tattcttgat gtgatacatt gcaagcttaa cccagatgac
    25381 aattggcttt ggtaatttca ttgacttgta ttgcgcaaaa tttattgtga aactatttct
    25441 ggcaacatgt agacgttgtt atcatacttt taaaaaatta tttttcacta aaaaaatacc
    25501 caaatgctta ctccgcaact attacattgg ccgcttttca ttcatttcca taatttgggt
    25561 tatttggaat ttgaaaattg acttgtaaag ttcattcgat tgccgctatc tctcaccaca
    25621 tcatttcgtt aatcaatctc tgattgattc taacatcact cgtcttcaaa cttattattt
    25681 atccccatta attcttctcc tcgaaagtac aaattctttt taaatgctgt gattgttttt
    25741 atgtctgccg gaactaaaat acaacgcaac attttcatta aggatatttt gaatttatac
    25801 gctagggcgg ttgcttttta tagcgggggc aatttaatac caccgcaaat aaaactgccg
    25861 caagcgcggc aggtatttta tttttgactt tgtaaaaaaa ccgaagactc gtgtagttgt
    25921 cgagcggcta tttcaaaagc aattgaacgg aaatatcttc atccaaatct tgccaatgta
    25981 tgccttcacc ttttccaata aagcgccaat tattcaattg tgcctgtgtg gcatttcgca
    26041 gacttacaaa ccattcaagt gggaatgata tttctctacc atcttccata aacagcatca
    26101 ttttgttttc tgtaaatttt acatcaacag cgttattcga tttatttatt gttaaagtat
    26161 tcattccact ttatattaaa tatgttttta tttccttcta caataatacg tatttctttt
    26221 aattcctttg aattaaagcc cgaagatttt actaattcaa caacacccaa attgaatttg
    26281 gcagtcctgc cggtactctc aacatgtatg tgtgcagaaa gatgctcgtt gctgtagaaa
    26341 aaaaatctat atccatatac aaaaagtaaa gttggcgttt tattgttttg ttgtgcgagt
    26401 ctgtaaaaaa tgatcttctg taaaacttaa attgattgag gcatgttgct aaattttatg
    26461 ctttgtttag aagccaataa cgtaaacgct gttatcgtta acagtagttt gccacaaatt
    26521 tcttggcttc cttggctgct ttttctcatc atcattctaa tcccattaaa cccaaaacta
    26581 taatattcaa aaactaaatt atttaaaacc aagttttcaa aaaataaaaa aggcttacaa
    26641 atgtattacc tcaccataag caacagcggt agcttccatc atggcttcgc tcattgtggg
    26701 atgtgggtgt acggctttca atatttcgtt gccggtggtt tcgagtttgc gggctactac
    26761 tgcttcggca attatttcgg taacgttagc acctatcata tggcaaccca aaaactcgcc
    26821 atactgcgca tcgtaaataa cttttacaaa accatcctta gcacctgcag cacttgcctt
    26881 gcccgatgcg ctgaaaggaa acttacccac ctttatttcg aaacctgctg ctattgcacc
    26941 tttttcggta tagcctacac tggcaatttc gggcgaggtg taagtacatc cgggtatgtt
    27001 gttataattt aatggctcgg ggtgatggcc ggcaattttt tccacacaaa taattccttc
    27061 ggccgaggct acatgtgcca gtgcttgtcc tttaacaata tcgcctatgg catatacacc
    27121 gggtatgttt gtttgataaa aatcgtttac caccaccttg cctttatcgg taatcacacc
    27181 tgtttcttcc aagccaatat tttcgatatt ggtagcaata cctacagcag ataaaacaat
    27241 atcgcaagta atttcctgtt cgcctttttt agtttttact tttactacgc attgtgcacc
    27301 cgatgtatcc acactctcaa ccgatgaatc tgtcattacc gttattcccg attttttgaa
    27361 actcttttcc aattgcttcg acacttcctc gtcctcaaca gggactatag cgggcataaa
    27421 ttctatcagg gtaacctttg ttcccatggc attataaaaa taagcaaact cactgccaat
    27481 agctcccgaa cccaccacta ccatcgattg aggttgttgt ggtaatgtca ttgcttcgcg
    27541 ataacctatc accttcttgc catcttgtgg taagtttgga agaatacgac tgcgtgcacc
    27601 ggtagctaaa ataatatgat tggcctcaag tgtttgtaca actccggcag catcggttac
    27661 ttcaagtttt ttgccgggct taagtttacc cgaccccatt atcacatcaa tcttattttt
    27721 tttcataaga aactgtaccc ccttgctcat gccatcggct acgccacggc tacgttttac
    27781 aaccttttcg aaatcggctt tgccgccttc tactgtaatt ccataatctg ctgcatgctg
    27841 caaatattcg aatacctgag cgctttttag caaagcttta gtaggtatac aaccccaatt
    27901 taaacagact cctccaagcg attcgcgctc cacaatagct gtttttaagc ccaattgcga
    27961 tgcgcgaatt gcagctacat atcctccggg tccactaccc aatactataa gatcatattt
    28021 cataaagtaa tttatatttt gttttttaat gcggctaaga taatcaacaa tttataaaca
    28081 aaagtcttca acagaaattt atgattttgc gagaaacttt tgttggtttt aggggttttt
    28141 agccttatgt ttgcacttta aaattaagcg ctaaataaat tgaccttaac atgaattata
    28201 aaaaactttt ctttctgttt ttgttttcgt ttttgtttaa atttcaatcg ctttcgcagc
    28261 tactcagcaa tggtttttat aaagtagaag ttgtaaatca aaatccggct ttgaaagctg
    28321 ccgaaggttt aacattcgac aaaaacggac gagggtatat ttgggacaaa cgagggtatg
    28381 tttttatact cgaaacaaac ggcacgctca atacaacgcc attacttgat ataaaagaag
    28441 aggttgccaa ttttggcgac aatggattaa atggatttgc attggatcct gattttttaa
    28501 ctaacggata tatttatgca ggatactccg ttgacaggta ttactatgat tattttggtt
    28561 tgccaggtta tgatcctgat tcatcgttta tcgatacttt gtcgtttggg cgcatcactc
    28621 gctttaaggc cgacataagt acaaattata caaccattgt tcccggcagc cgcactatac
    28681 tgcttggcga tagcattcac aatggcatgc cggttttata tttttcgcac agcaatggaa
    28741 atttagtttt tggtaatgat ggttcgctgc ttgccgcggt tggcgatgct gcagcttata
    28801 caacgcttga taccggttct aattcattca cttattggaa tcaagggtta gtggataatg
    28861 ttattgaacc aaaacataac gtaggagcgt ggcgtgctca aatgcttaca actttgagtg
    28921 gaaaaatatt gcgactcgat cctcaaacag gcgatggttt gcccagcaac cctttttatg
    28981 atactaccga tgcacggtcg ccacagtcgc gcatatatag cttagggtac agaaatccat
    29041 ttcgattttc tattgtaaaa aatactggtg atacagatat tactgccggc aatccgggaa
    29101 cgcttatact tggcgatgtg ggttggtcga agtgggaaga aatagatatc ataaaaacag
    29161 ctggattaaa tgcaggttgg ccatattttg aaggcttggc aaccaatcct ttttttgagg
    29221 tttcgaatga ggtaaacgaa gatgaaccca atccattata ttctcccggc atttgcgatt
    29281 ttcagtattt ccggtttcgc gatttattgc agcaggaaag taatttgaat cctccattcc
    29341 caaatccttg cgacagttca atcaacatga ctcagaaaca tcagtcttat cattttaggc
    29401 cgaccttgga atggcgacac aatcaaaaat taacccgtac cggaatattt agcggaggaa
    29461 ccggaaaggg aattccaatc aatataaatt tggcgaattc tcctataaaa ggcgaaatgt
    29521 ttgaaggtaa tgctgctgtg ggaggatttg tttatcaaac caataaagtg cctctagagt
    29581 ttcaaaatgc tgtgttcttt ggcgattatg gcgaaggttg gattcgttac atgcgcgcag
    29641 atgcaaacct taatattgat tcagttttct ccttcgatac cacagcatca acaatagttt
    29701 gccttacata taacgaaatt gatgcttgca tttactatgt aagttttcca gataaaattt
    29761 ataaaatatg ctatcccggc ttggtaaata atcctcctgt ttcgctacca acatccgata
    29821 ctatttatgg accatcacca ttaaccgtta attttgatgg tacggcatcg ttcgatcctg
    29881 aaactggacc tttgacttat cagtgggatt ttgccgacaa tggcgcagtg agcaacaatg
    29941 caatcgacag ccatacattt agccctggac ccggttttcc ggtgttgtat aatgtaactt
    30001 taactgttac cgatacggga atgttgcaaa acacaaagca tttacttatt catgttaaca
    30061 acactccacc aaaagttgat attacttctc cattaatcgg taccacctac agcacttcta
    30121 ttcctacact tatcaatatg acagcacagg taagtgattt ggaatcgcca aatgttcagt
    30181 tgtcatacag ctggaaggta gaaaaatacc acaatagcca tacccatccg gagtgcgatg
    30241 aaaccgaccc tgttacatat tgtattcttc cgcccgaagt ttgtctgcct aatgatactg
    30301 tatggtacaa agtttcactt accgttaccg acccccaagg cttaaaagga tatgattttg
    30361 tgggaattcc gccattatgt aatgttggat tgaataattt gttatttcag cagaacaaag
    30421 aaatactaat gtttccaaac ccaatagaaa acgaccaatt tgatattaaa tttttcgatg
    30481 agaagataag aacggtaaat aaaaaagatg ttcaaatctt tacatctaat ggagatgaat
    30541 ttagcccaaa aaattatact tttgggttta acaataattc aacctggagt gtaaaactaa
    30601 accatttgcc ttccggtctt tattttatta aaatcaatac tggtactaac tttgttaaag
    30661 ctaagtttat aaaattataa tttaaaataa acgtattttg tcaataacaa atgcagttgt
    30721 tggaacttaa tctatatact aaatctaatg cataaaaaaa atcgaaacat gctttataaa
    30781 aatgtaaagg caacttattt tactgctctt gtgctatttt ttctcatcac taaatgttat
    30841 ggccagggat ctcctgtttt aacgccttta ttcagcgaca atctggtaag ttcaggatgg
    30901 aaccaagttg ttggatttca atatgactca ttgggccgta tgtacgtttg ggaaaaaagt
    30961 ggtaaagttt ggtttgtaga tgccaacgga gtaaagagta ccacaccggt aataaactta
    31021 agcgatgaag taggtaattg gcgcgatcac gggttaaatg gatttgcatt agatccaaac
    31081 ttcaatacaa atggatacat ttatttatat tatactgttg attattatgc attaaaaaat
    31141 aaaggaaaac taaactatag taccaccacc aatgaatacc ttagagccac aattgtacgg
    31201 gttacaagat atacctgcga tgctgctaat gactttaaaa cagcacttat atctaccaga
    31261 ttagtgctta ttggtaaaac cattaaggac ggaattccat tgctgcatac atcgcatagt
    31321 ggaggttcac ttgtgtttgg tgacgatggt tcgttattga taagtaccgg tgatggttca
    31381 agtttcaatc aggtggatac aggcaatgca acctcttcat attggagtaa tggtttgtta
    31441 gattccatca ttcgtcctgc cgaaaatgtg ggatcatttc gctctcaaat gttggattca
    31501 tataacggaa aaattttaag agttgatcct gcaacaggga tgggattacc ttctaaccct
    31561 ttttatgatc ccaatagtcc tgatggtcct caatcaaaag tatggtgtct tggtttgcgc
    31621 aatccatttc gttttactgt aaaacgtggc agtggaagta cggatataac tgccggccaa
    31681 ccgggagtat taatgatagg tgaagtaggc tggcaggtat gggaagaaat aaatgtatcg
    31741 aaaaatggtg gagagaattt tggctggcca ttttatgagg ggctcacacc acatacgggt
    31801 tatttttcaa gccaggtaaa aaatttagat gaacccaata ccatcttaga cacagttaac
    31861 tgcggattag atttttatcg ttttcggcac ttaatgatac aagatacttt gtcgaacgat
    31921 tctatctttc ctcacccatg cgatgctaat caacaaattc cgaagtcatc acatccattt
    31981 acacatacaa gagctgcgat cgattacagg cataataccg ttgaaacacg tgttcctgtt
    32041 tttacaaatg gaacggcaaa tactgtgaag attgatgatc catcttcgcc aattgtaggg
    32101 caacaatttt ctggcaatgc ttctgtaggg ggagtttaca acagtgatat tcgcctttca
    32161 gctaaatatt tcaacactta ctttcatgct gattatggcg aaggctggat aaaaaatttg
    32221 aaattggatg gtaataacaa cattatttcc atagatactt ttgcggctaa cttgggtccg
    32281 gtggtatatc ttacattaaa cccaaacaat ggttgcctta cctatgttca ctacgaaaac
    32341 gaaataagaa aaatatgcta cacaggagtt gtaaattatc ctcccttagc caaagcacag
    32401 tcagataaaa tttttggaac ctctccgctc acagtgtctt ttggcgattc tttatcatac
    32461 gatcccgatg gtcaaccatt gtcgcgaacc tggtattttg gaaacggtga tagctccaat
    32521 gccagcaatc cttcatacat ttttacttcg gccaacaaca atcctatcac ttatacagta
    32581 atattgaaag tgactgatgc attgggattg acttcatttg acaccaccag ggtttatctc
    32641 aataatacgc ctcctattgt tgatataata acgctgccgg atactttttt atatactgta
    32701 aataattcta caattttaaa cttacgtgca aatgtatttg atgcacaaac gccaaccacc
    32761 gacttgattt acaaatggca aacaacattg tatcataaca atcataatca tcccgatcct
    32821 tatgaaacag ataaagaaac aacggttgat atttctccct taggttgcga tggtgaacaa
    32881 tacaattatt caatatctct aacagtttgc gatgctcagg gcttatgcgg taaggctgaa
    32941 gtttatgctt atcccgattg taatccggta agcaccttgt cgggaggttt tactgtttct
    33001 tcgaaagacg tatgtcgcaa cacagatatt atttttacgg acactacaaa aaatgccact
    33061 tcatggaagt ggtatttccc tggtggctat ccaagtcagg ctttaacaca aagcccttta
    33121 gtcaattata gttcaaccgg tacttttccg gtttacctgg tggcaaaaaa tgatttgact
    33181 caagattccg tggttcgatt aaattatata aaggtaaaaa gcttgccaac tattaccgga
    33241 attacaaatt atggaaatga tacagtttgc gggccatcaa gtttttatat cagggctata
    33301 accaccacca caaatctgat atttcagtgg tataaaaacg gggtgcctat atttgctgct
    33361 tttgatgaca gtttgttggt agactccgct gctaagtata cagtggtagt tactaatact
    33421 aatgggtgtt cctctacgtt ttcgaaaaca atttataatg gcagtttggt aacactttca
    33481 atagctacaa gccatacctt atatttatgt acttcggtcg atagcgcttt ggtttcatca
    33541 gtaaataacc ctgcatatac ttatcaatgg tataaaaatg gaatagcaat taatggcgcc
    33601 aacaatagca attattacgt taaaagtaaa ggtagttatt accttaaagt aaatgccaat
    33661 ggaatttgta caaataacct tacatcaaat acgcttacat ttacaaatag gccagatgtt
    33721 actttttccg tttcgggtcc tcttactttt tgcaattatg atagtgtaat acttgcatca
    33781 acaccctgtt ttgattgtgc atacaaatgg tataagaata acaccattat ttccagttat
    33841 ggaacaacaa agagtaatta caaagccaca accaatggta catataaggt aaaagtaata
    33901 gatcctcttg gatgtagttt gataagccca aattggcagg taacggtaaa tcctgttttc
    33961 actgttgcac aaagcgggcc gcttactttt tgcaatggtg atagtgttac actaacagta
    34021 acatcaaacc cggcattcaa gtataagtgg aaaagaaata atgtgtgggt gcctaatgcc
    34081 gaaacaaata aacttacaat caaaactgca ggtcaatact attgtcgcgt taccgacaca
    34141 ttgaataatt gcgtcaacag aacagtattc tacaacatag taatcaattg tcgcgaagcg
    34201 ggtatgtttg accttgataa tatttctctg gttccaaatc ctaccgaagg catatctaca
    34261 ttatcattta ttgcaaatac aaatgagcaa aatgccataa tatcgattta taacttacaa
    34321 ggtcaagaag tttggaaaga aaatgtgttt gttcaatctg gcgaaaatgg ttttgacatc
    34381 gatgcaggtg atttggcaag tggggtatat gaaataagga taagtgccgg caacgaaatt
    34441 tacaaagcaa ttaagtttgt aaaaatggat aaacaataaa tttcaatatt gtagaaaaac
    34501 agaaaggcag caaattattt gctgcctttt tttatttgat ttccaaatag cctataagtc
    34561 tgtaagccaa ttttgctaaa gtatattcac tcaaaatagt ccctttcata ggtgcgcatt
    34621 caacaatatc gaagccgaca acattttttg tttttataac tttctttaaa aaagttaaag
    34681 tttcttccca aaacaaaccg cggggttctg ctgtaccaac agccggcatt atcgaaggat
    34741 cgaagccatc agcatcaatt gtaaggtaaa cattttcggt taaatctttt acagcatcct
    34801 tcatccagtt tttattgctg cgcacatcat gggcgtaata ggtatgaatg tttttagatt
    34861 tttttatcag ttctgcttct tctatgcact gcgctcttat tcctatttgc acaagattca
    34921 agcccatatc gtgcacacga tgcattacac ttgcgtgtga aaacttattg ttatgatagg
    34981 tatcgcgcaa atcgctgtgt gcatctattt gcagcacgct aagattttta tattttgctg
    35041 cataagcttt tacaaaacca taagtaactg tatgttcggc acctaacgac acaacatatt
    35101 ttttatcgtt gagtaaccgt gttgtttcct gctcaatata atctaccgct ttggcatcta
    35161 ctttattttt aaaatccaat ggaggtaaag ttgcaatgcc acatttgcga aacgtttcga
    35221 tgcccaattc ctcatcatac aattctacaa attgcgaagc cttaaccatt gcacccggtc
    35281 ctttagcact gcctgccaaa taggaggatg tatgttcgta aggcacttgt tgtattacat
    35341 acttagatga tgagtagtcg taatattttt tttcttcaat gcccagaaaa ttgtctttgg
    35401 tcgatagtga ttttaaagca gccatttttt taaattatga attttgaaaa aaaaattatg
    35461 caagcacttt tgcaaccaaa tcggctgctt cttttaattg aattgctgag taaactttga
    35521 gcccgctttc gtcaattatt ttttttgctt cttccgcatt tgttccttga aggcgtatga
    35581 tgattggtac ttttatttca ccaatgtttt tatatgcatc tacaatgcct tgtgctacgc
    35641 ggtcgcaccg aactatacca ccaaaaatat taaccagaat ggcttttaca tttgggtctt
    35701 tcaaaataat acgaaatgct ttttctacac gtgctgcatt tgctgtacca cctacatcca
    35761 aaaagttggc agggtcgcca ccggcaagtt ttataatatc catagtggcc atggcaagtc
    35821 cggcaccatt taccatacag cccacattac catccaactt cacatagttc agttcgtttt
    35881 cgcttgcttc tacttctgtt gggtcttctt ccattttatc gcgcagcgct tcgtaacttg
    35941 ggtgacgaaa taatccgtta tcatccaaag atactttagc atctactgca attattttat
    36001 cgtcactggt tttgaaaaga ggatttattt cgaacatggc agaatctgtt tgcacataac
    36061 aattgtaaag tgccgccaca aattttacca tttccttaaa agcgttgccg ctgaggccaa
    36121 ggttgaaagc aatattgcgt gcctgaaatg attgaaggcc aacagaaggg tcaacttctt
    36181 ctttgaaaat caaatgaggt gtgttatgtg caacatcctc tatattcata ccaccttctg
    36241 ttgagtacat aattatgtta cgtcctgtat tgcggttaag caacacactc atataaaact
    36301 cttttgtttc gtttggaccg gggtagtata catcctgagc aatgagcact ttgtttactt
    36361 ttttgcctgc atcgcccgtt tgaattgtaa ccaacacatt gcccaaaata ttttttgtaa
    36421 tggtgcgcac atcatccatc gattttgcaa cctgtacacc acgctgttcg gtgccggcaa
    36481 tttttccttt gccacgacca cctgcatgta tttgagattt tactacccaa tactttgtgc
    36541 ccgtttgttt tgtcaattcc tcggctgctg caattgcacc ttcaacagta tcggcaacta
    36601 taccttcttg tatagccaca ccaaaactct ttagtatttc ttttccctga tattcgtgta
    36661 aattcataat cctttttttt aagtggcgca aaaatagata tatcaatcga atggcaaagg
    36721 gatatttatt ttaaacggtt gataataaat gctactgttt ttcaaaatta tcttcttcca
    36781 tttattcaat ctatttttta tctcagcaaa aaaaagatca attatattga aacactctcc
    36841 tattaaaatc actttatgag aaaaaagtta agcagcatat tgatttcgat ttttaggatt
    36901 gcaagatttg gatgcaatga attggtgaca attcgatgtg agtatagcac tcaaaattta
    36961 aggatacagc atattattaa atccccattc caaaattatt cactattatt tcttgtaatt
    37021 tactattgta aaaggcttaa acgaatcgcc attaatttcg aatgtaaaat agtgaaacgc
    37081 aaaaattgaa gcctaaaaac aatttatatt ttttataatt ttatggttta cccttttcaa
    37141 aactaagggc tcttttttgc attcaattga ttgtatgcgt aatcgcgtgc agtaatggca
    37201 ccggtgtttt tgggttccag ctgcaacacc atcgaaaaat cacgataggc tttttcccat
    37261 tcttttcgat caagataaac agaaccacga ttgaaatagg agtgaggtgt tgtaggattg
    37321 agttctattg ccttattgta atcagcgatt gcgctatcta tttttcccac ttttacatat
    37381 ataggcccac gtgaagcata tgctccacca tgttttggat ttagttttat agctacgttg
    37441 caatcctcta atgctttttc ccactgttca agagagttat atacaaaagc acgatttagg
    37501 tatgccaacg gaaaagcagg atcgattttt atcgattgat tataatcagc taacgcttta
    37561 tcccattgtt tcaggtttac ataaatgttt ccccgattcg aataagccag agaatttttt
    37621 gcctctatct ctatagattt agtatagtcc tctaatcctt tttgccaatt gccaaggctc
    37681 ttgtatgcaa aaccgcggtt ggtatacgct aatgcatacc gagggtcaac ttcagccgct
    37741 tttgttaagt cagcaattgc attttcatat tgtcggagtt cattataaat tttacctctg
    37801 ttaacataag cacgagcttt gttgggtgat ttttttataa tgtcggacca caaggaaaag
    37861 tcgcttttcc atacaatgtt gcgttggtac gtaagtgcag aattgaacaa aatgataact
    37921 ccaaaaatgg ctagagctat gttcatgttt ttatttttta gcaaggcaaa aacgcctgca
    37981 acaaaggcca gaaaaaaacc tactgatggc atataagtac ggtgctcaaa tattaaatct
    38041 gagattggaa taatacttga ctctataaca ttggtaagaa aaaaccaaaa tataccgaaa
    38101 gaaataattc tatttttgtt gaataaccat attccccaaa ccaatattgc taccaacgct
    38161 acaaaactaa tgatggtttt tatttcgaaa aaacctgaag ataaacggat gtcataatcg
    38221 aaattttggt tgatgggtaa aaacaaaagt tgtatatatt tcaacagtac ataaaattga
    38281 gtgaacaaat agtttgtaga tgatatttcg gtaaaatcat tgtaaacatc gggcggcaaa
    38341 ggtttaagaa cattgagcga aaacttattg gctacaaaaa aaatgaagcc cacacaagct
    38401 actataaata tggctatgtg tttacgactg aaattaaaag tgaatttaga tgattgaaga
    38461 aaaaatagct caactaatac aatggcaaat ggtaatgtaa aagcattttc ttttgtaaac
    38521 attgccaata tggctgtcaa caaagccccg gcaaagcaag caaatcgtga aatattgttt
    38581 tgggttaccc tcccttgtac gtatagtgtt atggttagta aatagaacaa tgtaactaac
    38641 gatgctaatc gctgtactat atatgttacc gattgagttg ccaacggatg tgaaacaaaa
    38701 agcaatgctg ctaccaaggc aaatatcctt ttatggtggt tggtagctgt ttctttaagg
    38761 gcattcgttg taaatattaa tgtagttagc caaaaaacca acagtgcatt aagaatatga
    38821 attataatat ttacaaggtg ataaccccat acattttctt tcccaaaatg ataattgact
    38881 gcaaaagaaa gataagaaac aaagcggctg tgattataat tccaaatagc atttatgtcc
    38941 gataattttt gaatttgatt attgttggtg atacttggaa tatcatcgac atgaaaacta
    39001 caatcgaacg aattggaata gataactgct cccaacaata tgataagtga aatggcaaaa
    39061 atattaaaat agtatttttt taaggaaggt gcctttggct cagtattttg tttgccgatt
    39121 ttcttttcag gtacagccgt ttttatttta ttttttttca tttttaatta ttgttgttgc
    39181 gcctgaaaag gtgcaacata aagagataaa tttttgagtc gtcaacataa atattttcgg
    39241 cccgcaaaaa aattgaggaa caaacttaca atattaccat taaatgcata gttgtttttt
    39301 tattttttat gtggattaat tagttctatc gcagcgtaat tttttttgta gtactatttt
    39361 ttggtatagc atactgtttt caatcatact tttaataatt atcgtgcgtg actgttttac
    39421 atagttatat tcattttgcg aatactgtcg gaatcgtatt gtctgatatg atacttacag
    39481 taattataac atgaaccatg taggcatagt attatcatag cgctataaaa tatattggct
    39541 ataggtggtt attacactat atgttgcaaa tggtagtata aaataatcga ttgtacttgt
    39601 acaaattggc aagagattga aagatcgcta aagacattat tttattattt gcgaaacttt
    39661 tggcaaaatc tatgcacaaa tgtttgattt atttttaggt tgatttaaat acctttgtgg
    39721 ctcatcaaca aaaaataatt atgaaaattg ctgtaataat tccttgttac aatgttgctg
    39781 ctcatattga atcggtggtt gaaaaaatac cagctaacat cgattggata atagcggtga
    39841 acgattgttc gaaagatgat actgcaaata ttctacttaa actcgccaag catagtaaga
    39901 ggataattta tctgcaacac gaaaagaatc aaggcgtagg tgctgcaatg ctaacagggt
    39961 ataaaaaatc tattgaactc aaagccgata tttcaataaa aatggatggt gacggacaaa
    40021 tggatgcaag ctatattccg gccctggtaa atccgttggt ggaagataag gcagatttta
    40081 ccaagggcaa tcgatttcac aatttgaggg cattgcaacg catgcctatt accaggcgca
    40141 taggtaacac cggtctaagt tttattataa aagcttcatc gggttactgg aatacttttg
    40201 atcctacaaa cggattcact gccattaaaa acgaaacatt aatggagatg gatttataca
    40261 gaattcacaa acgatattat tttgaaactt caatgctgat tgagttatac tatcgcagcg
    40321 cagttgtaaa agatatacct atgcatgcaa attatggtga agaaatttcg ggcttatcga
    40381 ggaccagagt tttttttgaa ttccccccaa agttgttgta tgcctttatc aggcgattgt
    40441 tactcaagta ttttttatac gaatttaatg tggcctccat atatttgctt accggcctgc
    40501 ctatgttttg ttatggcctt gcatttggaa ttaaaaacta cttaaagtac acgagtatag
    40561 gtttgcctgc acctacaggt accgtgataa ttcctacact attaattata cttgggtttc
    40621 agcttttgct ttcgggcgtg agttacgata taggtaatta tccaaagaaa taatgcaaac
    40681 gaatagcgta tgggttgcga gtggcaaata ttttttattt aacagccttg tgacatgcaa
    40741 ccaaatatat tattaagttt tattgtaccg gtttataatg ccgaaaatac attggcaaaa
    40801 tgtatagata gcattttggc tcaacccgaa aaagtttttg aaattatttt attaaatgat
    40861 ggctccgttg ataagtctca acttattatt gataaatatg ttttagagaa tccatcaata
    40921 gttaaagcat ttataaagga aaatagtgga cccggaagtg cccgtaattt tggaatacaa
    40981 aatgccaatg gcatctacat agcctttgtt gatagcgatg attatataga acccaattac
    41041 attaatgttg taaaaacaca tattgatgag catcgtgccg atatggttat cattggttac
    41101 aatagaatgt acaacaggaa gcaaagcctg ctcgaaagat ttcataagtt tagtaatttt
    41161 gatttatata atcaaccaat cgaaataaag acgaagccac aaatgctctg cgacattgag
    41221 gtggcttgtt ggttgagaat tgtgaagcgc gaattgtttt tggcagatga gaatttattc
    41281 ttctcttcaa atagtttggc cgaggacctt gaggcaagtt tgaaatggta tattaacact
    41341 aaagtaatag tagttagccc catcaaactt tataattata ttatcaaaac gaatacatta
    41401 aattttaaca ccggttttat tagtcaatac ataaacatta ttgaatcggt gtgtaattac
    41461 tataaagcaa agtcgcagtt taattattat ttcgaggagt tggaatatat gtttgccaag
    41521 catatgttga ttgcaaatct gctgcgattg cgagctgcta aacaggaggc aaagtttgaa
    41581 cagtttataa ctatgcgtaa atcattgttg cagtactttc ctcaatatgc taaaaacaaa
    41641 tactttaaaa acgatcctat ttacattcgg gccgctattt atatttcgta ttatttccca
    41701 aaattgtttt atccaatatt gtaatggaag gtagagttaa tttatctgta attatcccaa
    41761 tgtataatgc cgaaaaatac attgccaata ctattgcgtc tatacaaaat caacaaaagc
    41821 attcaatggt ttatgagata attattgttg atgattgtag caccgatcaa tcgcggcagg
    41881 tggttaaaga cctgaatagt gataatattc gactgattga gttaaaacaa aatggtggta
    41941 cttcgcatgc gcgcaatgcc ggtattgctg cagcaagagg cgagtggctg cagtttgtag
    42001 acagcgatga tgccatttgc agcgacctct attcgaagtt tgaagctgcg caacggcctt
    42061 taattaattg ctatgttttt tcactcatta tcgaagaaca taatcaaact atacaacgca
    42121 aaataataca agtaaaagac aaacgtgcca taggatattt ttatgccgtt tggaattttt
    42181 ttatcagaaa agaaatttgt gccgagttca atcatatttc gcggcaaaac gaagacgttt
    42241 gttttattgt tgacatgatg aatactaaaa acctcgaagt ggatttaata aaagatgcat
    42301 actatattct taatcgtaaa aatgatgcat cgaaaatggc caacttcagt gaaaaagaat
    42361 atttgaaaat gtataattat gtttatgggc gcattgacca ttgtgatgta cttaccaaaa
    42421 tgtttattct tgaaagtttt gttggtattg tgttttcaaa aggcatgccc ttgctcatga
    42481 gtttgcgtgt tgctatgaga accattttca aattatttta ctatttacct gcagtgtata
    42541 gtaatggtat aagaaattgt gtaaaaaata ctaccatagc ttaacgttaa taaaatataa
    42601 tcaaacattg gtgcagcaag atcataccat tacaaaaagt atcgacatta gtataatcat
    42661 acctatgtac aatgccgaac agtatatatc gcgcgccatt gcttctgtta tcaatcagca
    42721 ggcacataat tttaattatg aaattattgt aattgacgat gtaagtaccg atggctcctg
    42781 caaggttgta aaagcattaa ataatccaca cattaaactt atcgagttga cacacaatgg
    42841 cggtactgca aatgcccgca accagggttt gaaaattgcc actggccgat ggatacaatt
    42901 tttggatagc gatgatttta tttgtaatga cttgtatgca aaatttgaga agaaaatatc
    42961 cccgggttat aactgctatc tcttttctgt tatttacgag tatagcaatt acaacttaaa
    43021 gcaaaccata acatacattg atgataaacg tgcatttgga cattacggca gtgtgtgcaa
    43081 taagtttata aaaagagaga attgtttgcc ttttaaaaag gaattcagtt tcgaagatgt
    43141 gtgttttatt tttgatatga tgatggaaac tgaattaaaa attgcattaa ttgatgatgc
    43201 cttttactac tacaattgcc aaaacacttc gtcaaaaatg gcaaacttta atgctgtgga
    43261 atttgaaaaa atgtacgcct atgtttatag tcgaatcgac cgggccgatg cattaaccaa
    43321 aatgtttttt ctcgaaatat ttgttgctgt tttgttcgat aagcaacgtc cattttttat
    43381 gagtttacgt ataacaataa aaactctcat taaactttgg cgctacctgc ctaatgttat
    43441 tagaaatcaa aaccggggtt atattttaag tgcaagcatt gcgtaattct tgcagagatt
    43501 atttcgcctt gaaggaggct cgcaatgatg actttttgaa tcaacatttt aaaacggctc
    43561 ttttaaaagc ttccaaaaat ccggttccat atttcatatc tggttccaaa taatttcctt
    43621 ccgcccattc gttccaagac ttaataaaaa caatgcgttc atcgcttggt ttatgcttta
    43681 ctttttgaaa aatatcaatc acatgttgtt caaataactc aggtgttgct ttatgcagca
    43741 caagtccatt ttttccactg cgaggtgagt ggtcccaacc cgaaataata gttggaatac
    43801 aattcgtatc tgaatcttct ttccctgtaa aaaacttact ggcatttccg aagtcataca
    43861 catgaggtac tccaaaaatt ttactcttga ctaaattaag tgcaatggat ggaagcgatt
    43921 tttttaatcg caaaaagtca aatagcctat taatgtttac ggcatcaaat gatgcttcta
    43981 atattttatt gatgtcttca aacttattag attgcccaac aaaataaatt ccttttaatc
    44041 cattttttat ggcaagattt tgccattgtt caatgaaagc ttttgcatcc ggaattagta
    44101 gcggctgcaa aattgtaaat aatggcttgt cctgtacagt tatataccgc ttgtccagaa
    44161 aagcaggcag cacttcataa aaatgctttt caatgtcttc tgcgccagga tataattgtt
    44221 caatcaagac atttctatta gttaatccat gatgaaaccc tttccatgtt tcattggccc
    44281 aactaagaca aaatggaaaa tttggttcgc ctgattttag cacttcatta aatggccctt
    44341 ctaataattg ctttccgttt ccaaaccaat aatgccaata tgcaaaacct tctatgtcat
    44401 gttcctttgc catatctacc tgtgcctggc gagtttcagc tacccgcaaa tcatagtacc
    44461 ctaaatcggc aggaatgcgc ggttgataat gtcccggaaa atattttttc gctttactta
    44521 catttgtcca ctccgtaaag cctttacccc accaaatatc attttcaggt atcggatgat
    44581 actgtggaag atagaaagct atagctctaa tgttgccgac attcattaac gcttatttgt
    44641 tttcaaactt gtttttgttt atagatgctt catataatat agtgtcaatt gaattaacgt
    44701 aagtctccac accaaaaaat ttggctttac gcaggccatt caatgacaat gattcatata
    44761 aatttttatc agttagcact ttcaccattt cgtttttaag accttctgca gttccattat
    44821 atagcaatcc atttaagtta ttgtcaataa tttctaatac accaccggcc gaattagcaa
    44881 taacaggtat cccatagtcc atagcttcta ctattgttct accaaaagcc tccaattttg
    44941 tacaacttat tactaaatcc aaattttcat aaatctgatt tttgtccttt gtaaatggtt
    45001 gaaatatgac tttggcttca agttgaaaag caccaattaa tttcagtaaa cttttataat
    45061 aattttgatt tacgcctcca taaaaataaa gctcacaatt gggaattact ttaattaatt
    45121 ctgcaaatgc ttcaattgcc aaatgctgtt ctttacctgg cgatactgcc ccaatcattc
    45181 ctattttaaa attttggatg ggaaatgttt taaataaatt ggccgtaaat ttattgctag
    45241 caacgatgcc attataaacc acagattgac ggatcgaatt tttagcacac caatgttttt
    45301 ttaatatttc ggaaacaaaa atacttttgg ttgatttgtt tactaatttg gcaatttttt
    45361 ctctattggg ataatattta aaaccaaaac ctaaatcccc aaattctctg atatgccaaa
    45421 catgtggcac attaattttt tttgcaatag tgtaaccaat tgaaaccact gatgtgttgg
    45481 tataaatcac atcagcctca aaatcttttg ccgtttttat taattctttt aaaattgaaa
    45541 tgcgatttaa ataatagcta tattgatttt gaattctttt cagacgtgaa gcagttgcgt
    45601 aattaaaatc aacacaacgt ggaaaaggaa ttattttgta atttatttct gcatcaatta
    45661 attctttttc ataaacacca ccgaatggca gcagtactaa aatgtttctg tcagcttttt
    45721 tcctgatacc ttttaaggca gtaagcaaag attgtgaagc gccatacaaa aaggcatcgt
    45781 gtgcgaaaac taaaatattc atctaaacag ttttataaac atctaaatat tgttgagcga
    45841 caactgctat gctcatattt ttaacggcat aatcataatt attttgaccc aaattagtag
    45901 cagctaattt ttctgcttca tgaaaagctt tacacaacga gcctttttta taagggtcga
    45961 ataatgcatt gccagtatgt tttaaaagct ctccgatatt gccaacattg ggaccgatta
    46021 ccggtttttt aaaataaaat gctaatggaa cattgcctga gtttaaaata tgcttacgtt
    46081 gaattaaaac cacatcagca gcactaaaat agttttccaa aacatcatca ctaacgaatt
    46141 caattccggt ttttattttc tgtgatttta atctcatggt acgtgtctta gattctaacc
    46201 attttagaaa aacatttttt ggtgtatgct taatcaaaag cttcattaac ctgggtgcaa
    46261 aaagcagttt gttctgtatt tttgctttgg cgaattctga caaaaccatg tcggcttcct
    46321 ccatatctct atattcacca aacgtcagta caacaaactt atccaacggt atatttaatt
    46381 tttggcgggc ttcttttatg gtaatagtgt tgttatacat gttttcataa atatgatgag
    46441 gaataaaaaa atgtttaaca ctgctgttat tcaatcgctc tttatacaat tcaatttcat
    46501 ttcttcctaa atgaattatt gcatcagcat ttttttcaat taacttataa cacttatgcc
    46561 tgtttgcatc ggttgtataa tgaggcattt cattgtgacg cgtataaatt attttacagg
    46621 ttctttttat aacttgaata tgtttttcta acaattcgaa atcagaatct gacggaggca
    46681 ttttatcaaa cattgcatct ggccaatgaa aattaataat atcatagtga gcccagttat
    46741 tccaaaattc cttacgtccc caatttacat cacaacctaa gtctcttaat cctttcacca
    46801 aagttcttgt aaaaggattg tcgtttggat ttacttcagg ataaactatt aaaactttca
    46861 ttgtttatat aaatcaatta ttatttttat gttgttgtgt aactgatttc aatgcttgta
    46921 gataactatt accatgaagg cgagacggtt ccataacatt tccttcggcc cattcattcc
    46981 atgactttat aaaaactatt tgcgaagtat taggatttgc cgatactgct gcgaaagctt
    47041 tttttaaata caattcaaat tgttctgagg atgctgattc gcaaactata cctgccctgc
    47101 ctgaccttgg cgtgttatcc caattggtaa gtattacagg tatctctttg tctggtattg
    47161 gtttatttaa gtgttctgct actacatctt tataattata aattgcgggc ccttgatgag
    47221 gaaaatttct aatcaaatca gatattcgaa tattgaaatg tttaagactt aatctattaa
    47281 aaaaattatt tgcttgcttt gagcgtggcg taccagaagt gtaagaatcg aaacctaact
    47341 taagagcatt tgcacgggca ttaatggcat taaaatgaaa acctccaagc gaattttttt
    47401 cagccaggtt gtgccattgt ttaataaatt caggtatcaa aggtgaaccg aaaggttggt
    47461 aaatactaaa tagtagcttg ccgccttccc taatgtatct tttatcctga aaggctggaa
    47521 gacatgaatt aaaaaattgt tcaaaatcat gctcacctaa atactcttga gttgcaagaa
    47581 ctttatcatg caaaccatgc cacctacctg accaagattc gtttgcccat gctaaacaaa
    47641 atggaaaatc gggcttgccg ctactgataa cttcgttaat tggtctttca agaagttgct
    47701 tgccaccgcc aaaccaataa tgccaataac aaaaaccata taagcctgca tcttttgcta
    47761 attgggcttg tgcctctcta acttcaggaa ctcgcaaatc ataaaatcca aggtcgccag
    47821 gcaaaaaagg ctgcacatga cctttgaaca acggcttcgc ctttgcaaca ttcgtccatt
    47881 ctgtaaaccc tttaccccac cattcatcat tttcaggtat aggatgatat tgaggtaaat
    47941 aatatgctat tgtttttatt ccatttgtat ttgcattcat aaactattct acttactgtt
    48001 ttttactatt attttttttg tttggataaa tattaatttt ttttaaaaaa ttttataaag
    48061 cacgaaaagg ttttgtgatg cgccatgagt aagatgaata tatctgctca atttcgaaat
    48121 tctttttgtg caattctgcc ttatagcaaa tatttttgtt tctatatttt ttcaattttt
    48181 ttaaaaccat taatttgtaa agattatcaa acatttagaa attttgttaa tgcttagaag
    48241 aaattcagtc gaaataataa cagaaataat ttctatgtcg cttaacgatt gattgattat
    48301 cgaattcagg cattcagcta aataggatgc agtattataa actggaacaa ttacagatat
    48361 ttttggcatc gtcttacttt ttgaacaagt ttactacaaa aacaattgga gagagtaata
    48421 cctttccaag tttaaaagtt atggattcat aaatcgattc gattcgaata tctttagctg
    48481 taataatttt ttccttttcg ccaataattg attccaattg cttgacttta gttgccttct
    48541 cattaattac attttctaat gtctttactt tattttcttt ttcctctact actttattta
    48601 attctttaac attgttttct tttcgctcga ttgtattttc taaactctta acagtgtttt
    48661 ctttttcgtc aactatcttt tttaattcta taacattgtt ttctttttgc tcaattgtat
    48721 tttctttttc cgttactatt ttttttagtt ctgatgcatt gctttctttt tcctcaatta
    48781 ttttttttag ttctagtaca ctattttctt tttcctcaat tatttttttt aattccagta
    48841 cattggtttc caatgctgaa gcttttactt cctgttcctc aacaatttgt ttcagttgag
    48901 caattaatat ttctttttca ttctcaacgg ttgtattcaa aaggtttcga atatcactaa
    48961 tgtatttatc catggtaaaa ttatttttta ttcgttcgcg acccgccatt ccaagctttt
    49021 tcgctttttc tttattatca atcaatgtta acatagcttg tgccattccg gcaatatcat
    49081 attcttcaag caaaaaaccg gtttcgttat ttattattac atcgggtata ccgccatggt
    49141 aagtggcaat aaccggcaaa gccgctgccg atgcttcaag tacggctaca ggtgtgcctt
    49201 cactatcgcc cgagtcggca attacagaat gttgcacata ggctaatgca ttttccataa
    49261 gttgcattgt ttcttccggt ttcaaaatac ccggaaacga aacactgttt tcaataccaa
    49321 acgctcttgc aatattttta cacgtgtttt ccaatatgcc atcacctccc aatattaatt
    49381 tggcttcggg aacttttttc agcacttcgg caaaagccag caacgttaag tagggggctt
    49441 ttttatctac aaatcttcca atcccaaaaa aagttacgct gtcatatttt ggttttaaat
    49501 caaaaaactt tgcatttggt ccgtaatata tgagtgttat tttttctgcc ggacatccta
    49561 ttttgtgaag tttttgcgcc attatttttg atactgctat tactcccttg gcgtctgaaa
    49621 aaagaagttt atatttttcc tcatagtcag ccaatagttt atttccgctg gcatcatatc
    49681 catgaaagtg aacaacaaaa ggaatttgag cttttctaca tacttcgcgg attgaatatc
    49741 ctacaggacc aaattgtgca agtacaatat ctattttttc gcgttttagc aaacacacaa
    49801 tttcatcttc gagcgataat gattcttggg caacttttgt tcctcctgtt aattccgcta
    49861 ttcttttatt ctcagccttt attaattttc catcaaccat ggttggtggc ataccataat
    49921 gtaatagcaa ggctgccggc agaaaatcta cctgcgcttt cacaaatgtt tcggaatatg
    49981 cttctctatt tgtagtacaa atcgcaattc tcatcgtttt ctttttgcac agtttattgt
    50041 tacactttta tttattatgc aataattgaa aatcatttgc atacttaaaa agtcagtttc
    50101 ctttttattt gaaagattat tttttttcgc atgttccata aatagccttt tttgtttttg
    50161 ctatatgaat atttctccat cctttttcgt atagcatcct taaaaataga atcgtacttg
    50221 tatttgaaat gcctgttcaa tttgtccatt acagttaccc cttttagaat gatgtcgttt
    50281 ccctccatgc cgctccatat cgaattagtg tgaacacggt aaacgctcat tacactatta
    50341 aaatagcgaa ttttgccacg ttcggcattt aataaattca acaaccaatc tccggccata
    50401 attccattaa aaaatttcgg atcgatataa aatttccggt aaacaataga agccgtaggt
    50461 atacggtttt gatatatcaa tctctcaaat tcaaaatcaa agtcgctttt ataatcatta
    50521 taaataaagt cttttccata atatggtttt accaaagcat catggcaaca catactgtat
    50581 tcatcgttgg cttgcaaaaa atctatctgc ttttgcaatt tattctcatc ggtccagtaa
    50641 tcatcaccat cacaaagtgc tatgtagtta cccctgcatc ggttataatt aaaccttgga
    50701 ttaatacctc ttacgccttt cgaaaattga ttttcggttt gatacattgg aaaaataata
    50761 ttgggatagc gtgttgcata ctcctcaata atttcccttg taccatcggt cgatgcatcg
    50821 tcatgaatta caatttcaaa tgtaaaattt attttctgca ttaatatact atccaaacac
    50881 tgtttaataa attgtgaatg gttatacgta atgcatgcta tagaaaccaa tggtggcaga
    50941 gtatttttat tttttatttc taccatcatt tattttctat agtgcatgta acatgtattg
    51001 ttgccggcat aattattttt atcaaagcat tgcaatgtat tgatcttcgc cattataata
    51061 ataaaaagca caaaaggatt gctatttttt attttgtttg gagcacttaa tattttacaa
    51121 gtggttatac atttttcaaa tgacataaat agttgaacca ttttttttac tgtttaggta
    51181 acgatttttt gaatatcatt tttcgccata tttattattc ttgaaaaaaa atgttttctg
    51241 ctaatttcat ttctataact aatatactta atattttaat aatgcgataa ttttttgcga
    51301 ccactcatgc cgaatttttt gctatattaa ccacagtatc taactgttca aaattacgca
    51361 tatttgcgat aaaatgtatt ttgttttttt gattaattag attcaacact aatatttata
    51421 aatactttag caattgtgtt ttcgtagcca atattttttc aattcccaaa attatcgtgg
    51481 gactcaattc gtttttccag ttttcatcca ttgcccgctg tttataaact gagtatttat
    51541 tattatcatt tttatttcgc gatgcttgta tgaaatcaac agtagtttgt tccatcggta
    51601 tgcgggcaaa gttgaacatc ttttttattt ccgtatgcgt gtcagccaat aaattagaat
    51661 actgtgttat gtaaaatcgt tcgggatata ttgctgccaa ctcttcaaac atcagggcaa
    51721 cttccatcca cttattaaaa ccaaaaaact cttctatttt attttcgttt tttgctgttg
    51781 caaatagcca ctcttgttct tcgctccaac ccaaatcttt acgaaattcg cgtggtgtat
    51841 tgagccacga atttattacc gcaaacggat tgcggatgag cccgaacaat attatttctt
    51901 tatccttcat caaaaggttt ttaataatat ggtgatatct cacttcttta taaatgatat
    51961 gggtaaggtt tttatctttt ttaaatgttg gatatatacc tatatctctt tgctgcgatt
    52021 gatttataaa tgcatcatcg ctaagtgaaa tttgcgcgaa aaaattgctg atgtcttcgc
    52081 aggtagaatt ttcattcaaa tagtttttga atgcataaga aaacaatggt tgaaatttta
    52141 caactacgtg cggggcactg ttaaatattt gtgctaacca tgatgagccc gaccggggta
    52201 cgctatgtat gccaatcttt tgcatcacgt tattttataa ttgcttaaca tttgttttat
    52261 ttctgctttg gtattaaaca tcattacatc gatgattgaa agccacggta tgaaattatt
    52321 tccccattgc ttgtaattta ttgaattggg ttcagtaaaa tataattgta tatttctgtt
    52381 agcaaagtct tgtttattgt acagcgattt gccacctatt gtatttatgt aagtagtagc
    52441 ttttgtttgc gcgcaaatat catatagccg attttctttt ttcaattgtg ccgaattagc
    52501 aaaggattgt gagctgatgt gaaatcgggt ttttaaatct aggtattggg acacacacat
    52561 aacgcttgct gtagatagtt ctgaaatcat tttgtagtcg gtagcaatta cttcgcttat
    52621 gagtggaaaa actgtttcaa agtttggcgc ttttttatac gctaattcta tcgatgttaa
    52681 tattttatat tttgtctttg gcaacaaagg aatttcatta attaattttt tgtacgaggc
    52741 atttgccaaa ggcaccgtta tcattttggc ttcgttattt attagaatac ggttacggtt
    52801 aatccacccg ctatttataa attgcacatc atcgtaaaaa acaaattcat caacagcttc
    52861 tattaactga aaataaccaa tatacggaaa aaaatacggt tgcattattc caattttcat
    52921 cacactattt caatatcctt attatcattt tgcaaatcaa atcgatttcg gctttcgaca
    52981 attcaaaata aagcggcagg cataaaattc gtttcgaaag attatcggca acgggcattt
    53041 ttttatgttt tacataatca agtgtcgata gaagaggata aaaatatctg cgggtaaata
    53101 cattgtactt ttgtaagcca tcaaaaactt tcttgcaatc cttttctgtt ttaaatgcca
    53161 caggatagta cgaataatta taccctgcgt ttttcaatac caggggcttt tgtaattgta
    53221 agtgttggag atggttgtcg tataactgat ggtctgactt tctttttgcc agtatttcat
    53281 ccacatattt taaattcgtg aggcccattg cagcatgaaa ctcagagttt ttgccattga
    53341 taccaacgcc aataaatttg tcggggccgg catgaccaaa gttgcgcagg tgcgacattt
    53401 tttttatcaa tgctttgtct tttgtagtaa ctgctccccc ttctatagta tgaaaaattt
    53461 ttgtagcatg aaaacttcct atagtgatgt cgccataatt gaataccgat ttgcccttat
    53521 acatcgaccc aaagcaatgt gctgcatcgt atataacttt aagattatat ttttttgcca
    53581 gctttgcaat tgctgcaata tcgcagggat tgccgtatac atgtgtggca agtatagctg
    53641 aagttttttt tgttattgct gcttctatta ataatggatt gatattcata gtggcatcaa
    53701 catctacaaa caccggagtg caaccttccc acactatgct gcttgtggtg gcaacataac
    53761 taaatggcgt ggtaataatt tcacctttta gctccaaagc ttttattgct atttgcagtg
    53821 ctatagtacc attgcctaaa aaaagtaagt gctttacact caaatactct tttagcttta
    53881 attcaagttc ttttaccaat gggccgttgt tggttaacca attgcgtttc caaatatcat
    53941 ccacatattg aatgtattct tcgcgggggg gtaaaaaagg ttttgtaatc ggaatcatgg
    54001 agtatttaca ttaccggaaa ccaattccca attaaaatct aactgaacag gtgcccagcc
    54061 atgcacctta ctatgcacat taaaatatat tgccgattgt tgacgaaaaa caattttacg
    54121 tgaaccattc tcaatttcgc ttaatgctat agttattgaa taattgcctt gcgaaaaaat
    54181 aacttgtggc agtacagctt ttatacttaa taatccctca acctcaggca atacaatttg
    54241 ttgatcgaag ttgagtgtct ccgcaatatt tttttgctct ttatcataaa atattaaagc
    54301 aattcttgga ttgtaaaccg gtttaatgca atttaattct aagcttaaac aaagttcggc
    54361 accatgttcc actgtcaggg ttttttcatc tgtcgattct tttccttcac tcgataaagt
    54421 tattttttcg atactcactt tatcgctcgc cttgaaactt actatttggg tcttgaacat
    54481 ttcatagtat cttgtaatgc ctgccgccac attactcgat tgatatacac ttttaccttc
    54541 gttcaatatg cttatatcgg tgcacatacg cgatacttgt gataaaccat gtgttacaaa
    54601 tatcattgct gttttactgc gcaatacatc cattttgttg aaacacttca acacaaaacc
    54661 catatcacca acagccagta cttcgtctat caatagaata tcgggctcca tttgcgcagc
    54721 tattgcaaat cccaatcgca ctttcatacc cgaactataa ttttgtacag gagaatcaat
    54781 aaactttcct atttcggcaa attccaaaat agcgttaaat ctttcttcta cttctttttt
    54841 gctaaaacct aatagttgtc cattgttata aacgttttcg cggcccgaaa ggagtggatt
    54901 aaat
//
LOCUS       DAORQA010000005        53737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_104_length_53737_cov_36.479993, whole genome shotgun sequence.
ACCESSION   DAORQA010000005 DAORQA010000000
VERSION     DAORQA010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 53737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53737
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104_length_53737_cov_36.479993"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PK736_01015"
     CDS             complement(<1..329)
                     /locus_tag="PK736_01015"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HRC32002.1"
                     /translation="MRNIIALLKAYGVLKRLIIGIGLLIIVFIIGVMGFVFIEHYPIV
                     DAIYMTTITVASVGFGEVHPLTQSGKIFTSCLIFLSVTSYVYAITVVTSFIIEGEFRT
                     YFKHLRV"
     gene            512..769
                     /locus_tag="PK736_01020"
     CDS             512..769
                     /locus_tag="PK736_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32003.1"
                     /translation="MKQKLILLLLLLTTKVHAQNVMISNLNDPNEPSIMIAQQIISNE
                     NRDYGKYIERIDLTKLNLPNGTYLLKLEIDDKVEVQRQIKI"
     gene            840..1376
                     /locus_tag="PK736_01025"
     CDS             840..1376
                     /locus_tag="PK736_01025"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRC32004.1"
                     /translation="MVTSRITLRPIDAADLSTVHQLFSLPETDKYNTLGIPPTIDDTK
                     KIMDVWLDAQKIIPREKYIYYAMNEKKEFVGIVGINIGKPKYRLAELWYKLHPAQWNK
                     GYATEIVNSMIKYCFEDLKLHLVEAGCAFDNTASIKVLLKAGMQHEGMKRKLLPIRGN
                     WIDGNLYAILEEDFFNSK"
     gene            1482..2735
                     /locus_tag="PK736_01030"
     CDS             1482..2735
                     /locus_tag="PK736_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HRC32005.1"
                     /translation="MKSKLQRFWKILGPGLITGASDDDPSGIATYSQAGAGYGLSTLW
                     TAVIAFPLMAALQQMCARIGLVTTHGLTGTLKKYYPRSVLYVMLLFSFPAIVMNIGAD
                     IASMGAVGNLLFPAIDSTYFSVMFTALLLILIIYLPYHRIAAILKYLCLVLLVYLVVP
                     FLYKQDWLQILRSTVIPTLQFDKKYIGILVGILGTTISPYLFFWQASMEVEEMKHKKK
                     HLMVNKKIIHDMQQDVDFGMGFSGLVMFFIILTTGTVLYNSGIHQIDTVEQAALALKP
                     LAGNFAYLLFAIGVIGTGLLAIPVLSGSLSYIITETFGWQQGLDKKFHEAKAFYIVIA
                     ISLLLGLSLNYVGISPIEALIYTAILYGLTAPVLIAIILHICNNKKVMGEFTNSKTLN
                     IMGIAALIIMTLAGGALLYLQFTGQ"
     gene            2949..3818
                     /locus_tag="PK736_01035"
     CDS             2949..3818
                     /locus_tag="PK736_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRC32006.1"
                     /translation="MTNEQTAIRTTYFSIIGNTLLALTKGIAGTLGNSYALVADAIES
                     TTDIFSSFLVLFGIKYSNRPADKNHPYGHGRAEPLITFLVVGFLITSATIIAYESIIN
                     IRTPHKLPKAWTLIVLGAIIIWKEISYRLVVKKSIDTNSSSLRADAWHHRSDALTSIA
                     AFIGITVALIMGPGYETADDWAALFASGFILYNSYLIFRPALGEIMDEHFHDDLVAQI
                     RTEAMKVDGIIDTEKCFVRKAGMKFHIDLHATVDANITVKEGHEIAHKLKDTLRQQIP
                     VLGHVLIHVEPNE"
     gene            complement(4032..4880)
                     /locus_tag="PK736_01040"
     CDS             complement(4032..4880)
                     /locus_tag="PK736_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRC32007.1"
                     /translation="MNILTFDIEEWFHILDNEATSTETHWANFEVRIHQNMDRIHNLL
                     NQTNQKATFFCIGWIARKYPEIIKQINDNGHEIASHSDMHQLAYKQSKKAFEDDVEKS
                     IKHLEDIIGKKVISYRAPGFSVKEENKWVFEILMKFGIEIDCSIFPAKRAHGGFENFG
                     AAVPCWIDVEGMRIKEFPINLSTFLGKSLIFSGGGYFRLLPYHIIKRLTKNSDYVMTY
                     FHPRDFDPTQPMVPGLNYIRKLKSYYGLENALRKLEKLIKEEHFIDLKTADTQVDWTK
                     AKVLKI"
     gene            5421..9647
                     /locus_tag="PK736_01045"
     CDS             5421..9647
                     /locus_tag="PK736_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC32008.1"
                     /translation="MKKNIYNSIQVILACRLRLLLFACVFYLQINVSYAQCPPITVNQ
                     CGTLPVVGGGTIAPGSTAQFCIGDSIGVFNTNSGIIDSSWICWGDGTLSAFSGNFTDT
                     VKHKYNYKPDSCVNGGQASIDYWIVAQANCVNGVSINKAQLSLILKFYPHADFYFKDS
                     ICQYEIADLWCGQDCSTAYGWANISAPDSALITWHFGDGTDTSYYTYSNWINTPQHSY
                     TSPGNYTISMVIQQMGALPCGSDSVWHTLHVKAKSSAFPIVYANECAPDTFPGNIIML
                     NVDSLAWNVAPPNTVSNYLIPNPTFIVPNAGVTYIGAYLYGCWVDTNNVFISNTYYWD
                     TVLTFRPKPDLTFIAPSPDYCDSVHFVFQNYFSLNPAPTDSGYLFTILHNGTNIFSDT
                     SANPANYFTDTAGTYIVSALAWNDCDTLLFVDTFLITAPTVVAVSNDTSLCRWDASIG
                     LVGNPGAGTWSLNGIVVSNTFNPSTATQPYNYLVYDLGGCSIPDSLLITVNGLFVKAD
                     NDTSICDYASGFLLNAFPGGGIWQGQAVDPLGNYNPQNGNQAIDTNVYVYNDTSGCVI
                     TDTVFVTINSLNSASVTIPDSGCVNTALNFTVNNAGLNVVWNYGDLSALDTAHNATHN
                     YTSSGNFTVTLFSSDNNNCKDTITQNIFIDNPPNAVFTAAPLNGCAILPVIVNNTSIN
                     TYLPNYVWYYDNTIDTIFKPDTLFFGNGPNDTTQYPIQLVATNICTSDTFTVTVSVYS
                     LPHANFGANFTDTCSPALVQFNNISIGNTFSYSWYVNGLFYSSDSIPPPYYMVADSTD
                     SIYQVMLVAENICGKDSILKDIIIHPNQVKAFFNTSLSYGCAPLTVNFSSSVQANSTI
                     QWNFGDNTFATGDTVSHTYLTADTFVVWQRVDNYCGLDSTSHTIITFQQPPVSFTVDP
                     VLCGNLPVQFYNTSVGTLGAIWDFGDASNIDSINSAPSHLYSAPGVYNVTLIGISTGN
                     ACRDTFSIPINVLDYPTAVYAINPKDGCEPLQVSFADSSLKGKYFKYDLGNGDSLTGP
                     VNPQNYTYYFPGDFTVTLTSIDANGCMDDTSFSYVKVYPKPVVDFTYMQVPLCSTPAN
                     LQFTNQSTIATSFLWNFGGLGTSNHQDDSVLVNGDTTFAVTLIGSTVYGCTDTITKNV
                     VSHIKPTAKFTALPLEGCPPLEVDFLNQSQHYDQSWWTFGDGYSDITDSPTHIYDTTG
                     FYNVQLIVANDSVCYDTLLINNYIEVYPLPNANFSYVISPVGSLIPGNTVIFTDSSIL
                     ASAWNWTFGDGNGDAIENPTHQYDAGGNYIATLFITSDDGCTDTISKLIELGVFPTLY
                     VPNSFTPNNDGKNEMFEIKGSDILESNIIIYNRWGQPVFTSDNAIKNYWNGSDEFDRP
                     SPPGVYIYLLSVTKAGGDKYSLRGKLNLIK"
     gene            complement(9734..13042)
                     /locus_tag="PK736_01050"
     CDS             complement(9734..13042)
                     /locus_tag="PK736_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32009.1"
                     /translation="MKNLSCLFICLFFIFNSCVAQSLFHEDFETVDSVFSGGNPGFFQ
                     SIAVQTSGVASVLATYAIADSSLLVSNSFATLGYSKVVLSFNHICKMEFFDAARIYVS
                     IDNGQNWILLTGTEYLGSSSSFNVGNKFSEVSYSDWLPGQGSVLPDNSWFKYEAFDIS
                     AMAANKNSVMVMFTATDRNGTGILSRAGWFIDDVKVFSPSMAFNYSSACNATGMATVV
                     PYAGVAPYTYNWNTVPIQTNATATGLVPGYVACTITDINNNVFTDSVLIQNDLSVTAY
                     ILTIPTTQLSTNGAISVGATGGNSPYNFLWSNGATTNILTGLGVGIYTVTVTDSSGCV
                     LVDSFDLNIPIPPIAFTHTTVAALCNSNTGSITVTPVNGVPPYNYVWNTVPVQTTATA
                     TGLQGNSLLIIQITDANGTMGTGIIYAPFVDSVNINLITATPDTCAAGLGTSTGFAFG
                     GTPPYNYNWSTIPAQTNATATGLIAGLYILTVSDANGCAKSKSVYVPNITGFFGVLNS
                     FGTCYGNTNGSMVAVISGGMPPYTYAWSTVPVQTNATATGLTNGVSYYVQVTDANGCS
                     TVFGNYLSVTSQLFIVNNYSYNNCVDYSNTVSSQNGTAPFTFLWNTIPTQTNQTATGL
                     QYNTFYNCKVTDANGCVGWTQSNQWPPSACRINIQGTIFSDENQNCIYDGNDFPLNNI
                     ALGLNGTYNTWGFSNQVGFYDFHVPNSFYQLHHYDNYFIPLCLTFPQNINVPNGSIVT
                     IDIADTATKINDLSVSYCYSTNNVRGFATNLVVILKNEGTAPMSGTLYAKLDSNRLFF
                     NALPLQTNYVSATKTVIWNFANILPFQSRSFSIFEMIDPTTPLGLPVKDLFYITSTTN
                     DSNAGNDSCYSNGTVVGSYDPNLKQVSPPLYLQQNVDTVLSYTILFQNTGTDTARFIN
                     VYDTLDAALDTNYIFDVAASHAYILHRTGNILRFEFPNIMLPDSFHNELKSHGHIHYK
                     VRLKQGTLFNTQINNTAYIYFDFNSAIVTNTTVNLVVNNISIGEEEAALLQISPNPTQ
                     GLTNIDLNGYNNRSYALNITDLTGKILFEKTIEQNISKTIVDLSFLDNGCYIISLNDE
                     NGKVFSRNKVVLLK"
     gene            13255..15588
                     /locus_tag="PK736_01055"
     CDS             13255..15588
                     /locus_tag="PK736_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HRC32010.1"
                     /translation="MKTITLMLAALVINFCLSAQNFNMQLLSTLQYNNSCANIGGYVD
                     ALGNEYALVGVGDKLSIVDVTNAANPIEKFAVPMTDNLWKEVKTWQTYAYVTTETCCD
                     QVVIVNLQYMPDSIQYKTYNGDGAIAGQLTSVHSLHIDDGYLYLHGSNLNNGATIICS
                     LTDPWNPQYMSDVPTTYVHDGYVRNDTLWTCNIYDGKFTAVDVSDKYNPVVLNDQLTP
                     DAFTHNCWLNDAGNVLFTTDEVSNSFVAAYEVSDVNNIKFLDKIQTAPGSNSIAHNTH
                     VLNDFLITAWYTEGVTIVDAARPRNLIEVAKYDTYLQPGSGFAGIWGVYPYLPSGNLV
                     LASMDGYMYVIAPTYVRACYLEGMVTDSVTGVGIADANIEITGAGIIEQSKPTGDYAT
                     GTVNAGTYTVKVSKYGYFTKYFSNITLVNGQLTLLDVALVPKLAMALSGTVTNLSNGN
                     PIDSARVTLSDSEYEFIEYTDVNGVYTFNNLYESNYEITANKWLYSDSCLAPQTLTGT
                     NTINFAITPRIWDSFINDNHWTITSPQSPGQWVRATPVATYQNNNIANPANDVSDDCG
                     TKCFVTGNDGLNDNVANGTILNTPQLDLTPYSNPHIRFRYWLAFANDSLGNPGADLFT
                     ARISNGSTAAFVINTTSASVPAWHDTTFRVADFLTPTNIIKIRFTVADAGVPNVVEAA
                     IDAFEVFDMPLAVNEISAGTNINIIYSNNNILLQYNNLATAYTCIVTDEIGRQVFQTN
                     LQNHSGQIIMPEALTNGIYFVQIVSANGTGANLKFVK"
     gene            16118..18181
                     /locus_tag="PK736_01060"
     CDS             16118..18181
                     /locus_tag="PK736_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HRC32011.1"
                     /translation="MKLILAINFLFSGFLAALACLAIPVIVHLFNFRKFKKVQFTNVR
                     FLKEIKEETNSSRRLKHLLVLASRMLALAALVLAFAQPFIPVTMANKAAASKAVSIFI
                     DNSFSMDVAGKEGTLLQQATTKAKDIAMAYRPGDRFQLLTNDFAATQQRLLTREEFLE
                     EIQSIKSTTSSRSMKEIYLRQQEALNNAATSDKSSFIISDFQKSAYDLASIKEDSTIQ
                     TRLLLLPAQTASNLFVDTAWLANPLVQLNQSALLKMVLRNSGNKDLENVPVKLFINDI
                     QKSLASVNISAGGTTQAELTFTLNNAGIQRGQISITDFPVTFDDNYFFTFNIASAKKI
                     MAINGRIPSPYLMGVFNSDSSFVFENTSATQVNYGALKNNNLVILNNIQSISSGMAIE
                     LKKYVEGGGTLAIFPDSAADISSYNALLTSLRADAITSWSNMPDKVVKLDLQNDLFKN
                     MFDRLPDNMDLPTVLSHFEMSKLSSSTHLPLMSMNGNSALLSSYLFGKGKLYFFAVPL
                     NDVNSNFVNHALFAPVMYRMTLLVNKQPELSYVIGRDESLSLDNDDIGGEEVFHMLNK
                     KNNFDIIPQHTVNIGDLNIFFSDNIKQAGNYDVVLNNQPITSAAFNYNKTESVLEYWP
                     DEDLKSQIEKYGLKTFSSISNADGKITQIVNEQNEGIHLWKYCILATLLFLGLETLLI
                     RFWKT"
     gene            18178..19446
                     /locus_tag="PK736_01065"
     CDS             18178..19446
                     /locus_tag="PK736_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRC32012.1"
                     /translation="MILLIKNITVIDAASSYHMQCVDILIRDGIISDISKNIASEKNV
                     IVYDGSGKHISAGWFDMRVNFCEPGNEHKEDLHSGCEAAAAGGFTDVLLMPSTQPPID
                     NKAQVEFIKNKAQELNVEIHIAGCMTAKREGQELAELNDMKQAGAIAFTDDKRAISNL
                     HLMQIISTYAKNIDVPIIHFCEEKSLTHTVGVNESATTTSLGFKGTPSLSEEMAIQRT
                     LSITEYTNTPIHISCISTSNSVDLIRQAKAKKIMVTCDVAIHNLILDDAHIKTFDANF
                     KVVPPMRSKKDIDALIEGLKDGTIDCIVSDHTPQDMESKRVEFEFALPGIIGTQILFG
                     LAHTYLYQHLTLEVLLQKITTNPRNILKLQTITIEKNMAAKLTIFDKDAEWIVTENNL
                     KSKSKNTPFIGWRLKGRPLAIVNKNKFSEV"
     gene            complement(19550..21208)
                     /locus_tag="PK736_01070"
     CDS             complement(19550..21208)
                     /locus_tag="PK736_01070"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32013.1"
                     /translation="MKKLFLSTLLYVMLASISTTFAQTFNYDAGFDSLVISKQLEPTN
                     GLSEWPMYHNSTVSFGGRIYNYGIMDFDTVTVHLMVMDDNMITLHDTTIMIDFTGITT
                     GNFLNYEIGAFVAPAKGTYHVHCGTMIKNFVDENVLNDSAFAFFDVVDSTFAHDYTIY
                     MPATSLQGVNAPGQHSKMVQKYVLNKTDVMTAVTIKLNAPDFGDSTQAFIYRIDSATQ
                     YPDTILIAESDKYFFTANDTLGETTITLHFPNGGVSLPPDVYAIGVYEFNYSINLMAS
                     NRVFKDFTNFIYYDSLYGWGRIEQFNVFESYAIHPEFGCTMVLTSTTTSTICSNSSGS
                     ASVAYAGNSGNVTFLWSNGATVDNITGVAAGTYTCTVSDEGGCAIPFVVTIADLAAPV
                     IDSVVTTPDNGTNNGSAVCFVTSQSTPLTFVWSNGSTGNPVSNLAAATYTVIVTDING
                     CTKVGIVVIPSSASVDQLFNNFGIETFYNANEQLLSINGEWYANNNITIKILDLAGKA
                     VYSDSFLNTVSLNTNISTAGFAKGTYFINVQVGDKNSTNKIVLN"
     gene            21661..24030
                     /locus_tag="PK736_01075"
     CDS             21661..24030
                     /locus_tag="PK736_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRC32014.1"
                     /translation="MAKVWQVIKSTLIFLFDCISLRKVFKNKFLLWGARFILWTALYV
                     GAMETNFLWLCGYTPTLEEIRHPDVAIASELISTDSVIIGKYYYENRTPITYDKISPL
                     LVDALVATEDVRFYKHHGLDIYALISSMVSTAQGDQRGGSTITQQLVKNMFKTRKETN
                     QGLLQYIPIVKTVIYKSKEWITATKLELFYSKQDILEMYFNTVDFGNNWFGVKVASQN
                     YFSKQPLELKLEEAALLVGMLKATSSYNPIRNKKRSTERRNVVLGQMLKYDYIKQNTF
                     DSVSALPIKLTLKQKKRSDEHDSYIRVQAENILKKWCEKYGYNLYEDGLKIYCTIDSR
                     LQKFAEDATRDHMSKLQSQFYKQWGKQNPWRNENGGEIANFIEDNIKNTPVYADLVRQ
                     YGEKSDSVWKMLRAPKQMKVFTWSGAKDTTFSSYDSLRYYAKILRSGMMSLDPYTGYI
                     KAYVGGIDHKFFKFDQVFQGKRQPGSTFKPFAYLAAMDCGFSPCSRMTDKPIKIRYEG
                     GQEWEPHNANEKFSYRNMTLRRALGRSCNAITAQLTDSIGWGEVIEYAHKCGIRSKLD
                     SVPSICLGCSDVSLFELVNAYGTILNQGIRVDPMLIVKITDKNGKMLAEFKPQFEQMV
                     NPESAWLTLFMLMGSIQEPGGTAQSMWGYKVFDDGNEIASKTGTTSNESDGWFVGITK
                     NLVTGAWVGADYRSVHLRYGLGQGAHSALPIVGKYLEKALHSKGTGVRTGKLSKPPVK
                     IRKEYWCQQQDDIYLRDSIRSDSTIIAPSDSNLIFEIEVPKELLNDSVR"
     gene            complement(24166..25482)
                     /locus_tag="PK736_01080"
     CDS             complement(24166..25482)
                     /locus_tag="PK736_01080"
                     /inference="COORDINATES: protein motif:HMM:NF033539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HRC32015.1"
                     /translation="MSYKIEYSDDNVSAWGGFSLMKKFNDKIGLNSVLQNLPIPESKS
                     NNRFENQEIIESFLLSVWLGCYKFSHTHVLRLDDTLKKIFGWKQIPSDTTYKRFFQKF
                     NQGINNEVFPVLQEWFFNQLQFNNYALDLDSTVITRYGDLQEGNHKGYNPTKRGRASH
                     HPLFAFLGNERMVVNSWLRSGDTSSAHQCNEFLNETLSILKNKTIGLLRADSGFASDL
                     IFRHLEQKQIPYVIAGRMHYPLQDKIKQQKTWTAIGLGIWISETRYKASKWETERRVI
                     VIRQSVDLRPKATGKKLKLFDDMEYYEQYRYHTFYTNQTLPATQIWEQYKGRGDCENR
                     IKELKYDFALEGFNMKDFFATEAALRMVNLAYNIISLFRQVSSEKAKHQRLQTLRLNC
                     YAVGAWMTKKGNSTVLKLAVPIKKRKWMDGIFAKVDICSFPLSLVT"
     gene            complement(25662..27683)
                     /locus_tag="PK736_01085"
     CDS             complement(25662..27683)
                     /locus_tag="PK736_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32016.1"
                     /translation="MRLLFLFSILFCLRTCHAQTTFIKRIGLAGNDEWAHIDTTKEGN
                     IVLCGASSSSLNSPSDAIAMLIDTNGNALNTRIINSPNADLFKCIVQTFDGGFVACGY
                     TKDNGFGDIDAMIAKFDSALNVTWIKIIGNPERDEALHVIQTADSNFVIGGISYNNGF
                     SNNLVFKCDQNGNLLWQKVFGTSNNDVTHMIVEGFNGDIIAVGWAYYVGAGLHDNIIY
                     RLTKDGVFISGFDFGNPGDDALLSINKMSTYRYLVTGGTNTVGGYKNCLIGILDSALN
                     IIASTAFIINGKTVILRNGIENSDHSIITYVYDYLNNDSKMGMIKLDSNLNILWANWY
                     GDSLLCKSTGCTSFNSNGYVLSGFGEGFGVGDSNLLLIRCDTNGNTSQNCFTSPISIL
                     VYNYSFTSSNYSPPNSPILSYGTFITANNNQALIEQSFCIPLLLPICNIASSDSVFCD
                     KQAIDFYDLSTNNPTQWLWFFSGASPSISTMQNPVNIYYASPGSFDVILIACNSVGCD
                     TLIMPLFITELPQPPIPVVLVSGDTLLCSNATAYQWYNVNNTNVVLSTDSFYVPSVAG
                     NYYCLITDTAGCQSPSNVVGITTNLNQQNLMHLKYPSIIYDLTNGTLVINNNDKQVAL
                     SISNYMGQCLIKKQLAIGKTVIGVNEWSSGPYVVKFETQEGIGVFNFVK"
     gene            27817..27981
                     /locus_tag="PK736_01090"
     CDS             27817..27981
                     /locus_tag="PK736_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32017.1"
                     /translation="MMRDLLSKFQIDALDIRHLNREDIPLLGKLFTDGMLQKYSTRYG
                     WYILARCTKK"
     gene            complement(27983..28744)
                     /locus_tag="PK736_01095"
     CDS             complement(27983..28744)
                     /locus_tag="PK736_01095"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC32018.1"
                     /translation="MIKINENIWGHKYRLTWFKKFITKEQLGLEFGCGTGIMITSQLT
                     QDGYKCIGIDLDKASIDLGNKIFAEHGLPQDRLFCKDLNTFPDNHFDYILASEVFEHI
                     EKSDIDPIVELLKSKLKPEGIMIVTVPNGFGWHEFEEIFWAHLNFRRVFRFLYISQAF
                     NYIRLKTDTYVSKYPSTIAHSPHVRRFTFNSIKALCGKHNFEVFETRGSVFFSGGFSD
                     MLFSGVKPIMALTRKLGERWPSIATGFYLATRNKK"
     gene            complement(28731..30011)
                     /locus_tag="PK736_01100"
     CDS             complement(28731..30011)
                     /locus_tag="PK736_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRC32019.1"
                     /translation="MNSIRQKINSETFFQILQSANVQNLLKSIFSRIIALISSLVCSI
                     LITRSLNLDDRGQLSFILATVALFVVFSNLGIVNANIFHASKEPQNCPKLLVNSFWFA
                     LFVLLLTILCIAIFQNAGIIKSFTNFELFIISFSVFANLSLLLFKNILIGDNKINKVN
                     IIESVIKSFSLVAIVILFVLNKIDLHVCVVYYLLEPLLTIAVFTFVQREWIVNLRFDF
                     PYFKYNLKYGVRIYFLSLLGFLIMKADVFFIKRFLDNKQLGLYATSALVVENLGFIAS
                     VMSTLLLPRLIRIEGVRERFLVSRRQTFIASVVLLIIYSVAFVFAEFILSIFIKSNAI
                     LGVNVFRLLLVANFFLSVQSVNVQFLNSIGVPSRLILYWFIASFSNIILNFFFIKEYG
                     IEGAAFFSVVSYFIIYILVFFENKYQLKLHHDKN"
     gene            30268..32436
                     /locus_tag="PK736_01105"
     CDS             30268..32436
                     /locus_tag="PK736_01105"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC32020.1"
                     /translation="MTQLEGCASESDPITGALRFYTNGISVWNSNNTVMPNGTNLFGA
                     ASSAQSALIVPMPSDTSKFYIFTVSNYFTNGSPGAHYSLVDMNLNGGLGDVTTKNVLL
                     HNPTSEKLVATHQANNFDYWVLTHSFNGTTFYTYPVTNSGVGVPIISTCSPNFGGTSS
                     IGCMKLSPDGSQLAVARSGSNAIEIYNFDRYTGIVSGPAFTISSIAPNGLVYGVAFSP
                     NGKILYSTVNDNGFVYQFDLSSGNQATITASKTIVGNAPGSQYFRSMAMQLGPDKKLY
                     IVSDMDHYIAAISDPDIVGNGCNYNPYAVYLTSSKTGALGLPNCIETILIRKICAFNL
                     CAGDSAIFNSFSTAGLSQSYWNFGDTLSNSNIDSGFSASHFYGSPGSYTVQQIKVHAN
                     GIIDTIIQQITIHYKPYLIGSDDTTLCFGQQLTLTPVTNATNYTWQNGSTLSSFTVSL
                     AGTYIVTVTNTDGCKATDTTVINYISCGSPPIVSFYSSDTILCEKSAIDFFDISTNNP
                     TQWQWSFPGASPSSSTVQNPTGIYYPTYGAYTVTLVATNANGSDSLTITQYITVVANP
                     PAPSVTVNGSFMCCNNVAVWYQWYYNNVAIPNANAMCYNAMQPGNYYVLITDSNGCNS
                     GSGQIVISSLNETNVGPNFNAWYDATSGNVNVVMTKSKNEKATVTIYDVLGQTIKQIE
                     EENSENNKIMIIPAQFIAKGIYMIEVATKQKTSSGKLLIH"
     gene            32544..32843
                     /locus_tag="PK736_01110"
     CDS             32544..32843
                     /locus_tag="PK736_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRC32021.1"
                     /translation="MSVNSKHLATFLLGAAAALAAKKYMDMTPEEKEKMAADLKAKAN
                     NMKDEAEAMMEKANTYFEELKSKGSEAIKEHFPEAENFMKDFFEGKKKEDTPPVV"
     gene            33609..35657
                     /locus_tag="PK736_01115"
     CDS             33609..35657
                     /locus_tag="PK736_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRC32022.1"
                     /translation="MKIKFLILVFALNCWQFCFGQQGMWTWVNGTSVGNQNGVYGTQG
                     VADPNNTPPGTYEATEWTDHDGNFWMFGGSLNSSTIYADLWKYNPNNNMWTWVKGNGL
                     ANDAGDYGIMGVASPSNRPPAMSYGSASWVDSKGDLWMYGGLVVGCVRNHLWKYNIAT
                     NMWTWINGSSGCNNVAVYGILGVPSSLNTPGARSEFSASWIDEDDNLWLFGGNNLNDL
                     WRYNTATNEWTWMKGGNLPGQVNMHGIKGVPNSSNTPGSRFAFATWKDLNKKFWLFGG
                     HNYVPGHSYNDMWQYDPLTNIWTWMSGTNIPNDAGNYGPLCSPSINNIPAAGYENRAR
                     WTDECGNFWMYSGAGAYLNDLWQFNPTTLEWAFINGQSITWLQPPVYGIRNTPAISNT
                     PGTRTGVIGWRSTTGELFLFGGIVNVTDRNNDMWRFVPDTACMGCKSNFYPEVYFASS
                     DTVLCEKSCIDFTDLSLNNPIQWQWHFNGGNPNSSTIQNPTNICFNAYGDFVITLVAT
                     NAIGTDSAVYNSFIHVVQSPPQPTISINKDTLFSSPAVTYQWYFNNVLIPGATLPYYV
                     VQAPGNYYVIATDTNGCGNNSIQVIINSVNISEEQNSGIHIYPNPANDEIEILFDSKI
                     NTSSILIIYDPIGKKVFEKTITNTNRLNIDVAKFENGLYILELNDKSNFIHTSFLVQH
                     "
     gene            complement(35732..36073)
                     /locus_tag="PK736_01120"
     CDS             complement(35732..36073)
                     /locus_tag="PK736_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32023.1"
                     /translation="MKFKTLLTFVLICFATSQFVFAQSSKVESQKQTAKKTSDKYVCP
                     NCSLVANAAGNCTFCQMKTCKVGDFYCIHCGTVPADSGKCVYCTNKLVEMRLPDPNKK
                     IVRESNPSPAK"
     gene            36388..38244
                     /locus_tag="PK736_01125"
     CDS             36388..38244
                     /locus_tag="PK736_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32024.1"
                     /translation="MKKNILYSIVLIGILTNICFAQKEATKWYFGNNAGLDFISGVPV
                     PFSGSAMTMLEGVASIADTAGNTLFYTNGFQVWNKNNVVMPNGNGLSGNNNSAQAAII
                     IPVPNSASQYYIVTTPSNGNGTMDYSLVDMNLQGGLGDVTTKNVPLFNNSTEKVTSVQ
                     HANGIDIWLIGHEWNTNNFYSFQITPAGINPAPVVTSIGSVHNGVLGFAGYMKASHNG
                     TKIAYAITISLNIVELLDFDIATGIPFNPITFTNIQEAYGLEFSPDDSKLYATSENPQ
                     ILYQWDVNAGSPAAIIASQLAINASQGNEAFAALQLAPDGKIYLANRFSTMMGVINNP
                     NDAGLACNFVDDVFSLPFGSNQYGLPNFFQSYFDTIPASGTAPLVAFQSSDTVLCEKS
                     AIDFFDLSSGSPTNWNWYFPGAVPDTSTLQNPAGIYYASYGTFPVTLTVSNSFGQDSL
                     TILSFITVVPNPTQPVITQTGNDLCAPPFIYYQWFYNGTTIPQATNQCYTPTQPGSYY
                     VLVTDTNGCQGASLSVVITNLENANSPFGSVSITPNPVTNNFILTLSGTYFETLNLEI
                     SDAQGKKVYDEIIVKDTPTLSKTISTKLRSGMYFIKIQSGSKFIVKKFVVTE"
     gene            38448..40490
                     /locus_tag="PK736_01130"
     CDS             38448..40490
                     /locus_tag="PK736_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRC32025.1"
                     /translation="MKNKILLTFLTITVYACNLLAQPGAWTWLKGDPNLNSAFLGNNG
                     IQGVPAATNQPPFFYEAPTLMDTLGNLFLIGGGNGNGQWSDVWRYNTATNEWTWMKGT
                     GMGNATGVYGVKGVSSPTNQPADISLGSSYDNTKSGIFYIYGGSASISNNLWLFNPYI
                     NEWTWLNGNGNGGFTTPVYGVMGVPSPANSPGGRTEGLPSWCDSVGNFWIYGGSGGGT
                     YGDLWKWDVSISQWAWMGGSSNSLPANYGTKNVAAATNYPGARSFCSAWKLHNNELWL
                     GNGANSSGNSINDIWKYDINTNLWVWEGGSSLGSPNQNLGTKCNPAPSVMPNPSSEIK
                     ACWTFCDNLYAIHSSYNYTTAFNTVTKNFTWISGSQIPNAPGIYGTLGVPSVNNVCAT
                     KFGGAAWYKHPYAYVYSGIYGGTDMMRFEPDTTCGDYLNGCSQPVSVLAVNFASSDTI
                     LCEKNAVDFFDLSAGNPTQWQWYFPGASPDTSTAQNPTGIYYASYGTYPVTLVVSNGN
                     GSDSVTINIFIQVVSQPAKPIVTVNGNILCSSAASYYQWYYNGTLIPFATAQCYTTSQ
                     LGNYFVIVTDSNGCQNTSDLVLLNKVADNANSNQIQLIPQPAKENVSVQITNNIYGKL
                     LLQVTDLAGRVIYEKEQIKNYHYLETAIDCKAWPSGTYTVRVNLDGTFFSKRMVKY"
     gene            complement(40610..41284)
                     /locus_tag="PK736_01135"
     CDS             complement(40610..41284)
                     /locus_tag="PK736_01135"
                     /inference="COORDINATES: protein motif:HMM:NF023525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LipL32 family surface lipoprotein"
                     /protein_id="HRC32026.1"
                     /translation="MMKQILMKPFLIFLLLIFCLETSAQRLSKFESSQVINSDRIPYT
                     SQPGFYGCIADTIFLDSNNISSTVFYFYLPDSAIEIGVRAISPVPDITSPRPGDNTDS
                     SYYECATTNKGYFDPAIILQKGYLKTSKLPSNIFEWITIAQNDDSKDLPAQPNLLLRN
                     ALLRKQPNAKKKNSKLSPGYYRVVLTNNKSHYLIGTFLLQIGIVEKNKRLKLYNSIED
                     LEKSLK"
     gene            complement(41296..42018)
                     /locus_tag="PK736_01140"
     CDS             complement(41296..42018)
                     /locus_tag="PK736_01140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRC32027.1"
                     /translation="MTYPDLPFFISSNVCDSEIILTEAESFHAAKVLRLHSGDKINVT
                     DGNANYALAEITNIDARKCVAVPLALHTCVAKHNYNLHLAISPLRNMDRLEWMIEKAV
                     EMGIDSFSLVACSRTVKKNVNIARLQNIIISACKQSQKVSFPKINSLVSYNSFIESLT
                     GKSKLIAHCISDVPRQSYRSYIDKHDDVTILIGPEGDFSLNEIEQATSAGFESVHFGG
                     ARLRTETAGLLACSMLYARYEV"
     gene            complement(42154..46566)
                     /locus_tag="PK736_01145"
     CDS             complement(42154..46566)
                     /locus_tag="PK736_01145"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRC32028.1"
                     /translation="MAKRVFKITLWTLLIVLLLLTGFAFALRLPSVQTAVVHKITDFL
                     SAELKTKVTIGSVDFVFFKTFRFHQLYVEDEKQDTLLYVDKLDAAISLLSINGQKIHI
                     SKVTLSQGLFNLKRFKERKGLNLDIITDYFADTDTTPSKKKPWDVALDNIKVENIDFR
                     YKDYRWDDFTPLVDFDDIKVDNFTAELSDFYLHDSVYFNAENISFREQKGFAIEKLNT
                     KVGFTDSILHCDELTLKTAQSNVHGQIAFLFDQWEDFEEFIEKVRIQSSFTSSILSSN
                     DLAFFARELKGLNKIVTVQGDVKGTIDRLRGRNMIINYGAKCVMAGNIAMSGLPDIEQ
                     TYIEADLKKLVLNQKEIETIPQYPFYENEKIEIPQWLAKAGDLKFKGHFTGYIYDFVT
                     YGNIETALGNLSADVNLKLDTDNEKYSGNVSANEFDIGTLFDAEDVLGRTTFNLNVKG
                     KYFDINKMNAEVEGNIAKLHFNKYDYSNVDIKGSVAKKLFNGFLNVNEDNLGLVFNGK
                     VDFNQAKPIYDFEADIDHLKPYALHLLKGNGNGEFGGNVKMDFEGDNIDNMNGIALLR
                     DVSYLQDGKVIDIDSLSFVSTKYKDKYNISLKSDLIDASVDEVKKLSTAYYSLWNTIT
                     SYVPTLKPYEKKIFDENCQFEINIKNLDPVFNIFYPPLHIAPGTALSGEINSMTKHID
                     LFLSSPLVTWNDMRCEGLAIKGVDNKGKFDFVTNMQNFFLAPNIPIEKVTIDGNSDNQ
                     SAYIKLAIHDVDTATNKLVFAFNSEFRNDGITSIHIEPSTLVKVDTMLWKFNDENSTI
                     YDANGFTFNNVVLKSNYFNKIAINGNVGGNAAEPLRFIFKDFDVRPLNKLLVVYDVEF
                     GGVLNGNVNLMGLNDKPLVNGNLFIKDFAFFKDSIGDANIVSDYNSKSEVLHLEGNVM
                     RHGDSTLTFDGDYRIGEKDDYLDFDVSVQKIQLSTLQHYFDGLVSNLTGRGTGKLKIS
                     GTAKTPIITGKALVQKGKFTIDYLNTRYSLSDEIDFKEKYIGFNNIEVFDSLGNKGMV
                     SGKLYHKFFDDIKFDIHIDANNLLMMNTHAGQNDLFYGKAFATGKVDLTGTPDLTAMQ
                     IKLRTEKNTQINIPLSTPEEVSENDLITFEKHGAVELDKKEKISSLSGFDIDFEMDVT
                     EDAIIKLIFDEKVGDVIAGQGKGNLKLEITPDGAFNMFGEYTISKGDYFFTLQNIAGK
                     PFKLVKGSTIRWNGDPYQATIDIKALYTKPVSLADLLPDDSAAFARKVPVNVVLNLKN
                     NLFTPDVTFDIEVPNVDAATSSRIKRYIDTDDEKNKQVMSIIFLNKFQLPSALQGTQT
                     VNTGGNSGLSSLTEFLSKQLSDWVSQINKNVDINLSYTPGDVITSEQYNVLVSTQLLQ
                     DRLILNTNVGVNGNGGNENSANNSIVGEFDIEYVISKNGNFRVKAFNESNANSLINTN
                     QAPYTQGVGLSYRQEFTRYRELISKKKKNKTNSK"
     gene            46638..47660
                     /gene="tsaD"
                     /locus_tag="PK736_01150"
     CDS             46638..47660
                     /gene="tsaD"
                     /locus_tag="PK736_01150"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRC32029.1"
                     /translation="MNNGKYILGIESSCDDTAAAVCLDNMVLSNVIANQKVHEQYGGV
                     VPELASRAHQANIVPVVTEALRQAGIEKKKLSAIAYTRGPGLLGSLLVGSCFAKSLAQ
                     ALNIPAIEINHMQAHILAHFAIRKQSDATPTFPFLCLTVSGGHTQIVKVESALQMKII
                     GQTLDDAAGEAFDKISKVIGLPYPGGPLIDKNSKLGNPHAFKFNKSNIANYDFSFSGF
                     KTNVMNFIHNETQKNGNFIEQNLNDICASVQHTIINMLMDKIIKAAIDLQIKTVVIAG
                     GVSANSLLRSRLQETGNEMGWNVFIPPFEFCTDNAAMIAVAGFFKYNENQFSSLVDCA
                     TARYEW"
     gene            complement(47892..49103)
                     /locus_tag="PK736_01155"
     CDS             complement(47892..49103)
                     /locus_tag="PK736_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32030.1"
                     /translation="MITKHLRIKHFAFACLFCIGYVAQSQDNNDRLNLPGDNLNLAAV
                     LDIFQQSKTLEAFEQSLNSASSKINNLDLNNDNQIDYLRVVDKVDGTLHSIILQADLA
                     KDDVQDVAVIYVDKSNDQTRIQIIGDEDLYGKDYVIEPSTKNTARETPNPGYSGNDPI
                     TNNYTTNNYYNTNQSYSPDPSGWYIVNYLYTPGYMVWNSPWYWGYYPGWWRPWQPMFW
                     HHYHHHCYYDYAWNGWWYRPANNFYFQNHWRNTYYGHHRTSQIVVRNRANGVYNRTYE
                     KADAAPASPQGKPNFPTINKRNDAKPVEGRPMKNNDAQDIKPRDNPRDVNNNIEPAPR
                     PRNNDKAEPAPRPRNNNEKAEPAPRQDKKEESKPVVEPKKQTPEVPKVKPESKPRVVP
                     KKAEGKSSRRK"
     gene            49586..51145
                     /locus_tag="PK736_01160"
     CDS             49586..51145
                     /locus_tag="PK736_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRC32031.1"
                     /translation="MLSNNKTQHIPTEEQIEIDKKFFILQQSFSRNYEYIFSNDLAEK
                     TVIIIPSLTLDEEMLKTIKGVSHYEERMLCMLMLLRMPKTNVVYLSSVPIDSTVIDYY
                     LHFLPGITGYHARQRLTLLSCYDASRKSLTEKILDRPRLIERILHSVRNPAMAHIACF
                     NVTNFEKELAVKLNVPVFGTDPALLHWGSKTGSRIIFKRAGIPLSPGFEDLKDEGDIV
                     KALIDLKKNNPTLKRAVVKMNDGFSGEGNAVFNYGDIDVNDANITNIVTDELRNNLKL
                     VARNVPYQLFLDKFIDMHGIVEEFVEGEKKESPSVQIRINPASEIAIISTHDQILGGE
                     SGQVYLGASFPADPDYSKEIAQLGNLVAIELLKEGILGRFAVDFLSVKNDDKWLHYAI
                     EINLRKGGTTHPFLMLQFLTEGTYDYNNGVYLMPNGEARCYFASDNLARHEYKGLTPH
                     DLIDIAVCNNLLYDSVKQCGVMFNMIGALSQYGKLGVVCIGRNVEEAKAYYYKTEHVL
                     NLESMRTASKI"
     gene            complement(51368..51994)
                     /locus_tag="PK736_01165"
     CDS             complement(51368..51994)
                     /locus_tag="PK736_01165"
                     /inference="COORDINATES: protein motif:HMM:NF037410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32032.1"
                     /translation="MIEHDLGLAPNPFGKFCTLAVCKSEIRKSKNLSIGDWIIGTGSG
                     DIEAATKKRLKGKLIYAMQVTEKISLEDYWNDVRFQYKKPVMNGTLVTMFGDNFYHKD
                     ASGNWIQEDSSHCMTNGNCNPEHLRKDTRGKNVLISEHFYYFGNMAPAIPKELLDVCH
                     TTQGQKIVTPDELVTAFIDWVTSNHHQGIHGDPLNWCEYNPNQLSLTL"
     gene            complement(52016..52498)
                     /locus_tag="PK736_01170"
     CDS             complement(52016..52498)
                     /locus_tag="PK736_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HRC32033.1"
                     /translation="MSTANNIFISHLGEDDSHVQSLKQRLIDQGNNVRNFSIDSTKHK
                     NGRIPSDAVIQRYLRIRVKMSSTVVCLIGPDTHNSKWVNYEIRLAHLYGKRIIGIYTH
                     GSNNSVELPEAYKKYGSPAIGWNSLDKLSDSINGKNFPAENPDGSVATPLFQRTKVKC
                     "
     gene            complement(52502..53524)
                     /locus_tag="PK736_01175"
     CDS             complement(52502..53524)
                     /locus_tag="PK736_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004485635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA base hypermodification protein"
                     /protein_id="HRC32034.1"
                     /translation="MQRELETKYRKQEPVLILTKKAKPKLSVAYNTYWKFATERQNIF
                     FKRFNDESFPWTNDKILNQYKFTNVYRATDRVSQYLIKNVIYKGSQKPEEVFFRLLLF
                     KIFNRISTWEVLEYELGKISFKDYSFKRYDKVLSEVMQNRIPIYSAAYIMASGRSTFG
                     YERKHQNHLGLIEKMIKEKVPYKLQHCKSMSEAYTLLHSYPTIGGFLAYQYVTDINYS
                     TLTNFSEMEFVKAGPGAKDGIAKCFKDFAGYSFEDIIRLMAENQEQEFDRLNLDFKNL
                     WGRNLQLIDCQNVFCEVGKYARVAHPEIDDYSHRTRIKQKFADKEKEAIEFHFPPKWN
                     INHLIK"
     gene            complement(53527..>53737)
                     /locus_tag="PK736_01180"
     CDS             complement(53527..>53737)
                     /locus_tag="PK736_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32035.1"
                     /translation="SMVAIYNEKHSGNSIDYKKYLSTLQAIGLKRIAEQNGLSEKDNL
                     RLTVTPHISEVVYLILKGLTNRFTK"
BASE COUNT        16935 a        10072 c         9861 g        16869 t
ORIGIN      
        1 ttcactctta aatgtttaaa gtatgttctg aattctcctt cgataataaa agacgttaca
       61 acagtaatgg cgtacacata agatgttaca ctcaaaaaaa ttaaacacga agtaaatatt
      121 ttacccgatt gggtaagcgg atgtacttcg ccaaaaccaa ccgaagctac tgtgatggtt
      181 gtcatataaa tggcatcgac aattggataa tgttctataa aaacaaaccc catgacgcca
      241 atgataaaaa caataattaa tagaccaatg ccaataatca gtcgttttaa aacaccgtat
      301 gcttttaata atgcaatgat atttcgcatg aggcaaatat atcttttata aaatcactaa
      361 aaaacataaa tcattttttc tggaaaggaa tcacaattcc aaaatattta tgaatataat
      421 tttggcgttg gcactttcca atttatcttt gttcaaatat ctttttcgaa aaatttgata
      481 aaaagaaaat cttcaaaata aaagcaacaa catgaaacaa aaacttattc tgcttctact
      541 gttattaact acaaaagtgc atgcacaaaa cgtaatgata agcaacttga acgaccccaa
      601 cgaaccatcg attatgatag ctcagcaaat tatatcgaat gaaaatcgcg actatggaaa
      661 atatattgag cgcatcgatt taacaaaatt gaatttaccc aatggcacct atttgcttaa
      721 actcgaaata gatgataaag tagaagtgca aaggcagatt aaaatttagt gctgttggta
      781 aaggtttatt tcattcctgt tttttaaaat cacattttta ctattacaaa agaaaacaaa
      841 tggttacatc acgtattact ttgcgtccaa ttgatgctgc tgatttatcg actgtgcatc
      901 aattgttttc gctgcctgaa accgataagt acaacacact tggaatacca ccaactattg
      961 acgatacaaa aaaaataatg gatgtgtggc ttgatgcaca aaaaataatt cctcgtgaaa
     1021 aatatattta ctatgccatg aatgaaaaaa aagaatttgt tggaattgtt ggcataaata
     1081 tagggaagcc aaaataccgg cttgcagagt tgtggtataa attacatcct gcacaatgga
     1141 acaaaggcta tgcaacagag atagtaaaca gtatgattaa atattgtttc gaagatttaa
     1201 aattacatct agtagaagca ggttgtgcat tcgataatac tgcatcgata aaagtattgc
     1261 tcaaagcggg aatgcaacac gaaggaatga aacgcaaact attacccata cgcggtaact
     1321 ggattgatgg aaatttgtat gctatactgg aggaagattt tttcaactca aaatagtttt
     1381 tgaaatagtt tttctaaata aagacgttta cataaaaata atattctact tccgttttgt
     1441 ttgcattagc tttgcaaatg tagcaggaag aaaaagtgaa aataaaaagt aagcttcaac
     1501 ggttttggaa aatacttggc cccggcctca taacgggtgc cagtgacgat gacccgtcag
     1561 gtattgccac atactctcag gctggtgcag gatatggtct ttctaccttg tggactgctg
     1621 ttattgcgtt tccattgatg gcagccctac aacaaatgtg tgcacgcatt ggtcttgtta
     1681 ctacacacgg cctcacagga acattaaaaa agtattatcc ccgttctgtt ttatacgtca
     1741 tgttgctatt tagttttccg gcaattgtga tgaacatagg tgccgacatt gccagtatgg
     1801 gagctgtagg taatttatta tttccagcaa ttgattcaac ttactttagt gtgatgttca
     1861 cagcgttact tctcattttg ataatatatt tgccctacca tcgaattgcc gccattttaa
     1921 aatatctgtg cctcgtgctt ttggtttatt tggttgttcc atttttgtat aaacaagatt
     1981 ggttacaaat tttaagatca acagtaattc caactttgca attcgataaa aaatacatcg
     2041 gtattcttgt tgggatatta ggaacaacta tctctcccta tttatttttt tggcaagctt
     2101 cgatggaagt ggaagaaatg aaacacaaga aaaagcatct gatggtgaat aaaaaaatca
     2161 ttcacgatat gcaacaggat gttgattttg gaatgggttt ttcgggctta gttatgtttt
     2221 ttattattct tacaactggt acagttcttt acaacagcgg catacatcaa attgatactg
     2281 ttgaacaggc tgcattagct ttgaaacctc ttgcaggaaa ttttgcatac ctgttgtttg
     2341 ccattggtgt gataggcaca ggccttcttg ccattccggt gctgagcggt tctttatcgt
     2401 atatcattac cgaaacattt ggctggcaac aaggtctcga caaaaaattt catgaagcca
     2461 aagcgttcta tattgtcatt gccatttcgc ttcttttagg tttgtcgtta aattatgtag
     2521 gcatatcccc tattgaggct ttgatttaca ctgccatttt atacggcctc actgcccctg
     2581 ttttaattgc tattattctt cacatttgca acaataaaaa agtgatgggc gaatttacaa
     2641 actcaaaaac actaaatatt atgggaattg ctgcactaat aattatgaca ctggcgggcg
     2701 gtgcactatt atatttgcaa tttaccgggc aataattttt tgtttaagtc attttcaaat
     2761 ttagttaatg ccatcacaag tatctgcttc aactttactt tgcatcaatt ttcagtttca
     2821 atgttagagt agatgcacga aacttattta cattgtagcg caaaaaatat aaaactataa
     2881 catatgtttg cgtggcattt ttttatgaat attaatttaa acacttttcc atacgctgat
     2941 agtaattcat gacaaacgag caaaccgcca taagaaccac ttattttagc attataggta
     3001 acacattact tgccttaaca aaaggtattg caggcacatt gggaaattct tatgccttgg
     3061 ttgctgatgc tattgaatcc accacagata tcttttcttc gtttttggtt ttgtttggca
     3121 taaaatattc gaaccgccct gccgataaaa atcaccccta tggacatggt cgtgccgaac
     3181 ctttgattac atttttggta gttgggtttt taattacttc ggcaactatt attgcttatg
     3241 aaagcatcat caatattcgt acaccacaca aattgccaaa agcatggacg ctgattgttt
     3301 taggtgcgat aataatatgg aaagaaattt cgtaccggct tgtggtgaaa aaaagtatcg
     3361 ataccaacag ttcttcgctt agggccgatg cgtggcatca ccgaagcgat gcacttactt
     3421 caattgccgc attcatagga ataactgttg cacttattat gggacccggt tacgaaactg
     3481 ccgatgattg ggcagcattg tttgcatcag gatttatact gtacaatagc tatttgattt
     3541 ttagacctgc tcttggcgaa attatggatg agcattttca cgatgacctt gttgcgcaaa
     3601 taagaacaga agcaatgaaa gttgatggca ttattgatac cgaaaaatgt tttgtacgta
     3661 aggcaggaat gaagttccat atcgacctgc atgcaactgt ggatgccaac ataacagtga
     3721 aagaaggtca tgaaatagca cataagctca aggatacatt acgccaacaa attccggtac
     3781 tgggccatgt gctcattcat gtggagccaa acgaataaaa ttttacttga agtatctgta
     3841 acagtatacg ttggtcatgg atggtttggg gcacaatgta atatactctc agagctaata
     3901 atttaaaatc taaattaaat tgaaatgata aagtcctata atgcgagaag gtaacatttg
     3961 ctttggttcg aaaagtgctt agtataaatc cgaaaaatta aacgatatga aaagtatgta
     4021 agccccttat attatatttt gagaactttt gctttagtcc agtccacctg cgtatctgca
     4081 gttttcaaat cgataaagtg ttcttctttt ataagttttt ctaattttct taacgcattt
     4141 tcgagaccat aatacgattt taatttcctt atataattga gcccgggtac cataggttga
     4201 gttggatcaa aatcgcgtgg gtgaaaataa gtcattacat aatcggaatt ttttgtcagc
     4261 ctcttaataa tatgataagg caataagcga aaatagccac caccggaaaa aatcaaagat
     4321 tttcctaaaa aggtggaaag gttaatcgga aattccttta ttctcatgcc ttctacatct
     4381 atccagcaag gcacagcagc accaaagttt tcaaacccgc catgggctcg tttggcagga
     4441 aatatggaac agtctatttc aataccaaat ttcatcaata tttcgaaaac ccatttgttt
     4501 tcctccttta ctgaaaaacc cggagcacga tatgaaatta ccttttttcc aattatatct
     4561 tcgagatgct taattgactt ttcgacatcg tcttcgaatg cttttttact ttgcttgtaa
     4621 gcaagctgat gcatatcgga atgggatgcg atttcgtgac cattatcatt aatttgtttt
     4681 atgatttcag ggtatttacg tgcaatccat ccaatacaaa agaaagtggc tttttgattt
     4741 gtttgattaa gcaggttgtg aatacgatcc atgttttgat ggattctcac ctcaaagtta
     4801 gcccaatgag tttcggtgct tgtggcttca ttgtcgagga tatgaaacca ttcttcaata
     4861 tcgaaagtga gtatattcat tctttaattt ggccctgatg cttttatcaa tgcaccacta
     4921 ggttgatttt ttatcgaaaa taattttata taattaatag cacaaataca caaaaaacaa
     4981 caaaacatta aatcggagag acataaacca aattaattaa accatcagcg gcaatatttt
     5041 ccaaaagcaa gtgctgttat tggaataaaa ttgcgttttt aaatattcag atttttgtga
     5101 atgcacaaaa gtaatgtttt caatcattaa cacaaaaaca ctatttaaaa caagggcatt
     5161 tttatttgct aaacgaaatt ggcaacattg cgttgttagt tttgactttt ttgagtattc
     5221 tcgtttacta tttaattctt gcttcattac ctatgaaaga gcctatattt aaccctatgt
     5281 aaaaattgct tcaagacaca gtaaatatgg ctaaattaaa aaacattgaa acattaaact
     5341 tttattatat ttgtcctcta aatcaatagg ttgaatcagc caatgtatga ttactttttt
     5401 cagacaataa cataaaacga ttgaaaaaaa acatttacaa ttctatccaa gttatacttg
     5461 catgtagact tcgcttgctt ttgtttgcct gtgtttttta tttgcagata aatgtttcct
     5521 atgcacagtg ccctcctatt actgtaaatc agtgtggaac tttacctgta gttggcggtg
     5581 gaaccattgc gccaggctca acggcacaat tttgcattgg cgacagcata ggggttttta
     5641 ataccaactc cggcattatt gatagcagtt ggatttgctg gggtgatgga actttatcgg
     5701 ctttttccgg taattttacc gacactgtaa aacataaata taattacaag cccgactcat
     5761 gtgtaaatgg cggccaggca tctatcgatt actggatagt agcacaagcc aattgtgtaa
     5821 atggagtttc catcaacaag gcacagctat cgctcatact taaattttac cctcatgccg
     5881 atttctattt taaagacagc atatgtcaat atgaaatagc ggacttatgg tgcgggcagg
     5941 attgttctac cgcctatggt tgggccaata tttcggctcc cgattcggct ttaataacat
     6001 ggcactttgg tgatggaacc gatacatcgt attacactta ctcgaactgg ataaatacac
     6061 cgcaacatag ttacacatca cccggtaatt ataccataag catggttatt caacaaatgg
     6121 gggctttgcc ttgtggttcc gattcggtat ggcatacctt acatgtaaaa gctaagtcta
     6181 gtgcatttcc tatagtgtat gctaatgaat gtgctcccga tacttttcct ggtaacatca
     6241 ttatgctaaa tgttgacagc cttgcatgga atgtagcacc tccaaacaca gtttcgaatt
     6301 atttaattcc aaatccaact ttcatagtgc ccaatgcagg agtcacatac ataggtgcat
     6361 atctatatgg ttgctgggtc gatacgaaca atgtttttat cagcaatact tactattggg
     6421 atactgtttt gactttccgt cctaagcctg atttgacttt tattgcgccc tctcctgatt
     6481 attgtgatag tgtccatttt gttttccaaa attattttag tctcaaccca gcccctaccg
     6541 attcgggata tttatttact attttgcata atggaacaaa tattttcagc gatacaagtg
     6601 ctaatcctgc aaattatttt actgatactg caggcacata cattgtaagt gccctggcat
     6661 ggaacgattg cgatacattg ctttttgttg atacattttt gattacagcc ccaacagtgg
     6721 ttgcagttag caacgacaca tcgctttgca gatgggacgc atcaattggt ttggttggca
     6781 atcccggtgc cggcacttgg tcactcaatg gcattgtggt ttctaataca tttaatccat
     6841 caacagctac tcagccgtat aattatttag tttacgattt aggtggctgt tctattcccg
     6901 atagtttatt aatcacagta aatggcctat ttgtaaaagc cgataacgac acttccattt
     6961 gcgattatgc ttcgggcttt ttgttaaatg catttccggg cggtggtata tggcaaggtc
     7021 aggcagttga tccgttggga aactataatc cacaaaatgg aaatcaggca attgatacca
     7081 atgtttatgt ttataacgat accagtggat gcgtaattac cgatactgtt tttgtaacaa
     7141 taaattcgct caattctgca agtgtaacaa tacctgacag tggttgtgta aatactgctc
     7201 tcaattttac ggtgaacaat gcaggtctta atgttgtatg gaactatggt gatttatctg
     7261 ctctggatac tgcgcataat gcaactcaca attatacaag ctcaggtaat tttactgtta
     7321 cgcttttttc gagcgacaac aataattgca aagacactat cacacaaaac atatttatcg
     7381 ataatccacc taatgcggtg ttcaccgcgg caccattgaa tggctgtgcc atactgccgg
     7441 taatagtcaa caatacaagt ataaatactt acctgccaaa ttacgtttgg tactatgaca
     7501 acacaattga tactattttc aaacccgata cattgttttt tggaaatggc ccaaatgata
     7561 ctacacaata tcccattcaa cttgttgcaa ccaatatttg cacgagcgat acatttacag
     7621 taacggtaag tgtatactca cttccacatg ctaattttgg cgcaaatttt accgacacat
     7681 gctcaccggc cctggtgcag ttcaacaata tttcaattgg caatactttc tcatacagtt
     7741 ggtatgtaaa cggtttgttt tatagcagcg atagcattcc cccaccatac tatatggttg
     7801 ccgactctac cgactctata tatcaagtta tgcttgttgc tgaaaacatt tgtggcaagg
     7861 atagcatcct aaaagatatt attattcacc ctaatcaggt gaaggcattt ttcaatacca
     7921 gtttgtcgta cggctgtgca ccactgacgg taaatttttc gagcagtgtg caagccaaca
     7981 gtactataca gtggaatttt ggagacaaca cttttgcaac aggcgatacc gtgtcgcaca
     8041 cttacctcac tgccgatact tttgtggtgt ggcaaagggt tgacaattac tgtggtttag
     8101 attctacaag tcacaccatt attacgtttc agcagcctcc tgtttctttc actgtcgatc
     8161 cggttttgtg tggcaactta cctgtgcaat tttacaatac ttcggttggt acacttggtg
     8221 caatttggga ttttggcgat gcatctaata tcgactctat caattcggca ccatcgcatt
     8281 tatatagtgc accgggggtt tataatgtta cgctcattgg tatatctacg ggcaatgctt
     8341 gtcgcgatac attttcaatc ccgataaatg ttttagatta tcccactgct gtttatgcta
     8401 ttaatcccaa agacggctgc gaaccactgc aggtaagttt tgccgattca tcgctgaagg
     8461 gaaaatattt taaatacgat ttaggaaatg gtgattcgtt aacagggccg gtgaatccgc
     8521 aaaattatac ttactatttt ccgggtgatt ttacagttac acttacttct atagatgcaa
     8581 atggttgcat ggacgataca tcatttagtt atgtaaaagt ttatccaaaa ccagttgttg
     8641 attttactta tatgcaagtg ccattgtgca gcactccggc aaatttgcag tttacaaatc
     8701 aaagtactat tgcaacttca ttcctttgga actttggcgg gctaggcact tccaatcatc
     8761 aagacgattc ggtattggta aatggcgaca ccacatttgc agttacttta attggaagca
     8821 ctgtttatgg ctgtaccgat acgattacaa aaaatgtagt ttcgcatata aaacctacag
     8881 caaagtttac tgccttacca ctcgaaggtt gcccgccatt ggaagtagat tttttaaatc
     8941 agtcgcagca ctatgaccaa tcgtggtgga cttttggtga tggttacagc gacattaccg
     9001 atagcccaac tcatatttat gataccacag gattttataa tgtacaacta attgttgcta
     9061 acgactcggt gtgttacgac actttactta taaataatta catagaagta tatccactgc
     9121 caaatgcaaa tttcagctat gttatatcac cggtaggaag tctcattccg ggcaatacag
     9181 ttatttttac cgatagcagt atacttgcct cggcatggaa ctggactttt ggcgatggaa
     9241 atggcgatgc tatagaaaac ccaacgcatc aatatgatgc gggtggaaat tatattgcta
     9301 ctctttttat tacgagtgat gatggctgta ccgataccat ttcgaaactc attgaattag
     9361 gtgtgttccc aacattgtat gtgccaaatt cttttactcc aaataacgat ggcaagaatg
     9421 aaatgtttga aataaagggc agtgatattt tggaatcgaa cataataatt tacaaccgtt
     9481 ggggacagcc ggtgtttacg agcgataatg cgataaaaaa ttattggaac ggtagcgatg
     9541 aatttgaccg gccatcacca cccggtgtat acatttattt gctctcagtg acaaaagccg
     9601 gtggcgataa atatagtttg cgaggcaaac tgaatttgat aaaataataa tcaaaagtgt
     9661 tttttttgat tcgaataaaa ctttgtttca taagcacttt tcaactaata tttcaaccgg
     9721 gattcaaaat aaactatttc aaaagtacaa ccttgttgcg tgaaaatact tttccatttt
     9781 catcatttaa gctaatgata tagcaaccat tatcgaggaa agataaatca acaattgttt
     9841 tcgaaatgtt ttgttcaatc gttttttcaa acaatatttt acccgtcaaa tctgtaatat
     9901 ttaaagcata agaacgatta ttataaccat tcaggtctat attagttaaa ccttgagttg
     9961 ggttgggaga aatttgtaat agcgcggctt cctcctctcc aatcgaaata ttattcacca
    10021 ctaaattcac agtagtattt gtaactatgg cactgttaaa atcgaaataa atgtaagctg
    10081 tattatttat ttgtgtgttg aataatgtgc cttgtttcaa tcgcactttg taatgtatgt
    10141 ggccatgact ttttaattcg ttatgaaaac tgtcgggcaa cataatgttg ggaaattcga
    10201 agcgcaatat atttccggtt ctatgcagaa tgtaagcatg cgatgccgct acatcaaaaa
    10261 tatagtttgt atccaaagca gcatcaaggg tatcgtaaac atttataaat cgtgcggtgt
    10321 ctgttccggt attttgaaac aaaatagtat aactcaaaac cgtatctaca ttctgttgta
    10381 aatataatgg tggcgacact tgctttaaat tgggatcgta tgagcctaca actgtgccat
    10441 tagaataaca tgaatcgttt ccggcattag aatcatttgt tgtggatgtt atataaaata
    10501 aatctttaac aggtaaaccc aatggcgtgg tgggatcaat catttcaaat atagagaagc
    10561 ttcgtgattg aaaaggtaaa atattagcaa aattccatat cacagttttt gttgccgata
    10621 cataatttgt ttgtaaagga agcgcattaa aaaaaagtcg attgctatcc agtttagcgt
    10681 atagtgttcc gctcataggt gctgttcctt cgtttttcaa aattaccaca agatttgtag
    10741 caaaacctct tacattgttg gtagaatagc aataagaaac gcttaaatca ttaatctttg
    10801 tagcagtatc agcaatgtca attgttacaa tacttccatt tggcacatta atattttgag
    10861 gaaatgttag gcacaacggg ataaaataat tatcataatg atggagttga tagaaactat
    10921 tgggaacatg aaaatcgtaa aaacccactt ggtttgaaaa gccccaagta ttataagtac
    10981 catttaaacc taatgcaatg ttatttaatg gaaaatcgtt tccatcataa atacaatttt
    11041 ggttttcatc gctaaatatg gttccttgaa tattaattct gcaagcggac ggaggccatt
    11101 gatttgattg cgtccaacca acacaaccat ttgcatcggt aaccttacaa ttatagaaag
    11161 tgttatattg caaaccagtt gcggtttggt ttgtttgcgt gggaattgta ttccaaagga
    11221 aagtaaatgg tgcagtgcca ttttgtgaac tcactgtatt agaataatcc acgcaattgt
    11281 tgtaactata attattaaca atgaagagct gagaggtaac actgagataa ttaccaaata
    11341 cagtgctgca cccatttgca tcggttactt gaacataata cgacactcca tttgttaagc
    11401 cagttgctgt tgcatttgtt tgaaccggta cagttgacca ggcataagta tatggcggca
    11461 tgccacctga aataactgca accatagaac cattagtatt gccataacac gtgccgaaag
    11521 aatttagcac cccaaagaag ccggttatat ttggcacata aaccgatttt gattttgcac
    11581 atccgttcgc gtcagaaact gttaaaatat aaagtcctgc aataaggccg gttgcagtcg
    11641 catttgtttg agccggaata gttgaccaat tgtaattata aggtggtgtg cctccaaaag
    11701 caaagccggt ggatgtgccc aacccggccg cacaagtatc gggtgtggcg gtaattaaat
    11761 ttatatttac cgaatccaca aaaggagcat aaattatacc cgttcccatt gttccatttg
    11821 catcggtaat ttgtataatt agtaaactat tgccctgtaa tcctgttgca gtggcggtag
    11881 tttgcaccgg aaccgtgttc caaacataat tgtatggagg cacaccattt acaggtgtaa
    11941 cggttatact tccggtatta gaattacata aggctgcaac tgtagtatgt gtaaaagcaa
    12001 tgggcggaat tggaatattc agatcgaagc tatcaaccaa aacgcaacct gatgaatcgg
    12061 taacggttac agtataaatt ccaacaccga gtccggtcaa aatatttgtt gttgcaccat
    12121 tcgaccataa aaaattataa ggcgaattgc caccagtcgc acccacgctt atagcaccgt
    12181 ttgttgataa ttgtgtggtt ggtattgtga gtatgtaagc tgtaacagat aagtcatttt
    12241 ggataagtac agaatcagta aacacgttat tattaatatc agttatagtg caagcaacat
    12301 agcccggcac caaaccggtt gcagtagcat ttgtttgtat aggcactgta ttccaattat
    12361 aggtgtaagg agcaacaccg gcatatggca ctactgtagc cattccggtt gcattgcaag
    12421 cactcgaata attaaaagcc atgctaggcg aaaacacttt tacatcatca ataaaccaac
    12481 ctgctcgcga caaaatacca gtaccatttc tgtctgttgc agtaaacatc accataacag
    12541 aatttttgtt tgcagccatt gccgaaatat caaaagcttc atatttgaac caggaattat
    12601 caggtaaaac cgacccttga ccaggcaacc aatcagaata ggaaacttcg ctaaatttgt
    12661 ttccgacatt gaaactacta gaactaccca agtattctgt ccctgttagc agaatccaat
    12721 tctgcccatt gtctatggaa acatagatac gggcagcatc aaaaaattcc attttgcaaa
    12781 tgtgattaaa tgacaacacc actttgctgt aacccaatgt tgcaaaagaa tttgaaacca
    12841 acagtgaaga atctgcaatt gcatacgttg ccaaaacgct ggcaacaccg gaagtttgca
    12901 ctgctatact ttgaaaaaat cctgggttcc cacctgaaaa aactgaatca acagtttcga
    12961 aatcttcgtg aaacaatgat tgtgcaacac aactattgaa aataaaaaac aaacagataa
    13021 ataaacagga taaatttttc attggatggt gtttaattgt tgcatcaaat gtaacaattt
    13081 gtctaagttg tacaatttcg ttttgaaaag aataattgct attacattaa tttttacgct
    13141 acctttttgc tccaagtcac gcctttgcaa ttttttgtta atgtacattt tcgaatgaaa
    13201 atctttcata taatttgaac acatttgtaa actgaaacac aaaacaaaat ttcgatgaaa
    13261 acaattactt taatgttagc tgcgcttgta ataaattttt gcctgtcagc ccaaaatttt
    13321 aatatgcaat tactttcaac attgcaatac aataactcgt gtgccaatat tggcgggtat
    13381 gtagatgctt tgggaaacga atatgcactt gtaggtgttg gcgataaact ttctattgta
    13441 gatgttacca atgctgcaaa ccccatagaa aaatttgccg taccgatgac tgacaattta
    13501 tggaaagaag ttaagacatg gcaaacctat gcatatgtga ctaccgaaac atgttgcgat
    13561 caggttgtga ttgtaaattt acaatacatg cccgactcta ttcaatacaa aacttataat
    13621 ggcgatggtg caatagccgg tcaattaaca agtgtacatt cactgcacat cgatgatgga
    13681 tatttatacc tgcatggcag caatcttaat aatggcgcca ctattatttg ttccctaacc
    13741 gacccatgga acccacaata tatgagcgat gtgccaacaa catatgtaca cgatggttat
    13801 gttcgtaacg acacattatg gacatgcaat atttatgacg gtaaatttac tgctgttgat
    13861 gtgtcggata aatacaatcc ggttgtactt aatgatcaac ttacgcccga tgcttttact
    13921 cacaactgtt ggcttaatga tgccggcaat gttttattca ccaccgatga agtttcgaat
    13981 tcttttgtag cggcatatga ggttagcgat gttaacaata taaagtttct tgataaaatt
    14041 caaactgctc cgggttcaaa ttccattgca cacaacactc atgtactgaa cgattttctc
    14101 atcactgcat ggtatactga aggcgttaca atagttgatg cagcaaggcc gagaaactta
    14161 atagaagtgg caaagtatga tacctactta caacccggta gcggttttgc aggcatttgg
    14221 ggggtgtatc cttatttgcc aagtggcaat ctggtgttgg caagcatgga tggatatatg
    14281 tatgtaattg cgcctactta tgtcagagct tgctatctcg aaggtatggt aaccgacagt
    14341 gtaaccggag tgggaatagc agatgcaaat atagaaataa ccggagccgg cattattgag
    14401 caatccaaac cgacaggtga ttatgccacc ggaactgtta atgcaggtac gtatacggtt
    14461 aaggttagta agtatggtta ttttacaaag tatttttcca acattacttt ggtaaatgga
    14521 caacttactt tgctcgatgt tgcgctggtt cctaaattag ccatggcatt atcaggtact
    14581 gtaactaact tatcaaatgg caatccaatc gacagcgcac gtgttacgtt gagcgatagc
    14641 gaatatgagt ttatagaata tacagatgta aatggagttt atacatttaa taatttatat
    14701 gaaagcaatt atgaaataac tgctaacaaa tggttataca gtgattcatg tttagcacca
    14761 caaacgttaa caggaaccaa tacaataaac tttgcaatta ctcctcgaat ttgggattcg
    14821 tttatcaacg ataatcattg gacaataacc agcccgcaaa gccccggaca gtgggtacgt
    14881 gcaactcctg ttgctactta ccaaaacaat aatatagcga accctgcaaa tgatgtgagt
    14941 gatgattgtg gcacaaaatg ctttgtaaca ggcaatgatg gtttaaatga taatgttgca
    15001 aatggcacca ttctaaatac tccacaatta gacctcaccc cctactccaa cccacacatt
    15061 cgttttcgat attggctggc atttgccaat gattcgctag gcaacccggg tgccgatttg
    15121 tttaccgccc gaataagtaa tggaagcact gcggcatttg taataaatac tacttcggct
    15181 tcggttcctg catggcacga tactactttt agagttgccg attttttgac gcctacaaac
    15241 attataaaaa tacgatttac tgtggccgat gccggagtgc ccaatgtggt agaagctgct
    15301 atagatgctt tcgaagtttt tgatatgcct cttgctgtaa atgaaatttc ggcaggaaca
    15361 aatatcaata ttatctatag taacaataat attttgcttc agtataataa tttagctaca
    15421 gcgtatacat gtatagttac agatgaaatt ggccgccaag tttttcaaac taatttgcaa
    15481 aaccattcgg gacaaataat tatgcccgaa gcattaacca atggaatata ctttgtgcaa
    15541 atagtaagtg ctaatggaac aggagcgaat ttgaaatttg tgaagtagaa ataatttgca
    15601 gccttgccat gaaatggact tattaaaaaa cattgaaaat ttcataaaaa aatgaaaacc
    15661 attaaatatg ccttgacctg aaatttttca ttaatcgtat ggccgatttt ttttgtgttc
    15721 tgaattttag tgataaaaca caattcattt tggagcaatg aaaatatttc gcaaaatgat
    15781 tgacaataaa tcagagcgaa aataaaagtt acacataggc ataatattct ccttcacatt
    15841 ttgcattcaa aagcagaaag aaatcaacga aaatcaactt gaactgcgca tgcttttctt
    15901 cactaaactt ttggagtggc ccatttttca aactggaaat aattgaaatt tttacaacct
    15961 gatttgataa aaaatcttag aaccaaacaa ccaacaactt aataaccaat caaccaataa
    16021 caaagtaacc caatcaacca ataacaaagt aacccaacaa ccaataacca aaatttaatt
    16081 tccttcaatt ggtatggtta tctatatttg cgctcgtatg aaacttatat tagcgattaa
    16141 ctttttgttc tcgggttttt tagccgccct ggcatgtttg gcaatacctg taatagtgca
    16201 cttgttcaac tttcggaaat ttaagaaagt gcagtttacc aacgtgcgtt tcctcaaaga
    16261 aataaaagaa gaaacaaact caagccgaag gctgaaacat ttattggtac ttgcttcgcg
    16321 catgttggct ttggctgctt tggtgctggc atttgcacaa ccatttatac ctgttactat
    16381 ggccaataaa gctgctgcaa gtaaagcagt aagcattttc atcgacaact cgttcagtat
    16441 ggatgttgca ggcaaagaag gaactctgtt gcaacaagct actaccaaag caaaagatat
    16501 tgctatggcc taccggcctg gcgacaggtt tcaattgctc acaaatgatt ttgctgccac
    16561 acaacaacgt ttgttgacac gcgaagaatt tttagaagaa attcaaagca tcaaaagcac
    16621 cacttcatca cgcagcatga aagaaattta tttgcggcaa caagaagctc ttaacaatgc
    16681 tgcaacatcc gataagtcat catttattat ttccgatttt caaaaatcgg cttatgacct
    16741 ggcatctata aaagaagaca gcaccattca aacacgcctg cttttattac cggctcaaac
    16801 tgccagtaat ttatttgtcg acaccgcatg gcttgctaat cctcttgttc aattgaatca
    16861 gtcggcttta ttaaaaatgg ttttacgcaa ttcgggcaac aaagatttag aaaacgtgcc
    16921 tgtgaaatta ttcataaacg atattcaaaa atcattggca agcgtaaata tttctgcagg
    16981 tggcactaca caggcggaac ttacttttac tttgaacaat gcgggcatac aacgcggtca
    17041 gatttcgatt acagatttcc cggtgacatt tgacgataac tattttttta ctttcaacat
    17101 agcttcagca aaaaaaataa tggcaattaa cgggcgtata ccttcacctt atctcatggg
    17161 tgtattcaac tccgactcgt catttgtttt tgaaaatacc agtgccaccc aggtaaatta
    17221 tggcgcgcta aaaaacaaca atcttgtcat actcaataat attcaatcga taagcagtgg
    17281 tatggctatc gagcttaaaa aatatgttga aggtggtggc acactcgcta tttttcccga
    17341 ttcggctgct gatattagtt cttacaatgc tttattaaca agccttcggg ccgatgccat
    17401 tacttcgtgg agcaacatgc ccgataaagt agtaaagcta gatttacaaa acgatttatt
    17461 caaaaacatg ttcgaccgct tgcccgataa tatggatttg ccaacggtgt taagtcattt
    17521 tgaaatgagc aaactatcgt catctactca cttacctttg atgagtatga acggcaattc
    17581 agctttattg tcatcatacc tttttggtaa aggaaaactt tattttttcg cagtgccatt
    17641 gaacgatgtg aacagtaatt ttgtaaatca tgctttgttt gctccggtaa tgtatcgtat
    17701 gacattgttg gtaaacaagc agcccgagtt gagttatgtt attggccgcg atgaatcttt
    17761 atcgcttgat aatgatgata ttggtggcga agaagttttt catatgttga ataaaaaaaa
    17821 caacttcgat ataattccgc aacacactgt aaacattggc gacttaaata ttttctttag
    17881 cgacaacata aaacaagccg gcaattatga tgtggtttta aataatcaac ccattaccag
    17941 tgcagcattt aattacaaca aaaccgaatc ggtactggaa tattggcccg atgaagactt
    18001 gaaatcgcaa atagaaaagt atggattaaa aacattttca tcaataagca acgctgatgg
    18061 aaaaatcacc caaatagtaa atgaacaaaa cgaaggcatt cacctttgga aatactgcat
    18121 cttagccaca ttattatttc ttggattaga aactttactt attcgatttt ggaaaacatg
    18181 atattgctga taaaaaacat tacagtcata gatgctgcat cttcatacca catgcagtgt
    18241 gtggatatat tgatacgcga tggaataata tctgacataa gtaaaaatat tgcttccgaa
    18301 aaaaatgtaa ttgtttatga tggaagtgga aaacatattt ctgctggatg gtttgatatg
    18361 cgcgtaaatt tttgtgagcc cggcaacgaa cacaaagaag atttgcattc aggatgtgag
    18421 gctgctgccg cgggtggatt tacagatgta ttgttaatgc catcaacaca acctcccatt
    18481 gataataaag cacaagtaga atttattaaa aataaagcgc aggaattaaa tgttgaaata
    18541 cacatagcgg gctgcatgac agctaaacgc gaagggcaag agttggcaga attgaacgat
    18601 atgaaacagg cgggtgctat tgcatttacc gatgacaaac gtgccattag caacctgcat
    18661 ttaatgcaaa taattagcac ctatgccaaa aacatcgatg tacccatcat acacttttgc
    18721 gaagaaaaat cgctaacaca tactgtaggt gtaaacgaaa gtgcaactac cacttcttta
    18781 ggatttaaag gtacaccatc gttgagcgaa gaaatggcaa tacaacgaac tttatcaatt
    18841 accgaataca ccaatactcc cattcacata tcgtgtataa gcacatcaaa ctcggtggac
    18901 cttataagac aggcaaaggc gaaaaaaata atggtgacat gcgatgttgc cattcacaat
    18961 ttgatactgg acgatgccca cataaaaacc tttgatgcta actttaaagt tgtgccacct
    19021 atgcgttcta aaaaagatat tgatgcattg atcgaaggat tgaaagacgg caccatcgat
    19081 tgtattgtga gtgaccatac tcctcaagat atggaaagca aacgtgtaga atttgaattt
    19141 gctttgccag gcattattgg tactcaaatt ttattcgggc ttgcacatac atacttatat
    19201 caacacctta cgcttgaagt gttgctgcag aaaatcacca ccaacccaag aaatatttta
    19261 aaactgcaaa caatcacgat tgaaaaaaac atggcggcaa aactcaccat tttcgacaaa
    19321 gatgcagagt ggatagtaac cgaaaataat ttgaaatcga aatcgaaaaa caccccattt
    19381 attggttgga ggcttaaagg caggccgttg gcaattgtga ataaaaataa attttcggaa
    19441 gtgtagtatt ctactactcg ccatttcaaa tatcaaacac gcatttgaag tgcaaaaaaa
    19501 atcccggtaa gtactcactc accgggattt tttatttgcc cgatatcttt tagtttaaaa
    19561 caatcttatt agtagaattt ttatcgccca cttgtacatt tataaagtaa gttcctttgg
    19621 caaaacctgc tgtactaata ttggtattta acgatactgt atttaagaat gagtccgagt
    19681 aaacagcctt tcctgctaaa tccagaattt tgatggtgat attgttgttt gcataccatt
    19741 cgccattaat actcaataat tgctcatttg cattataaaa agtttcgatt ccaaaattat
    19801 tgaataattg atcaacactg gccgatgatg gaataaccac aatgccaact ttggtgcatc
    19861 catttatatc ggtaacaata actgtatacg ttgccgctgc aagattgctc accgggttac
    19921 cagtacttcc gttactccaa acaaatgtca aaggtgttga ttgtgaagta acaaagcaaa
    19981 cagcactgcc attgttagtt ccattatcgg gagtagtaac tacactgtcg ataacaggcg
    20041 ctgccaaatc tgcaatggta acaacaaatg gaattgcaca accgccttcg tcacttacgg
    20101 tgcatgtata cgtacctgcc gcaactcctg taatattgtc gactgtggca ccgttgctcc
    20161 ataggaacgt aacatttccg ctgttgcctg catatgccac cgaagcagaa ccactcgaat
    20221 tgctacaaat agttgatgtt gtggttgatg taagtaccat ggtgcaacca aactcagggt
    20281 gaatagcata agattcgaac acattgaatt gttcaatgcg accccaacca tacaatgaat
    20341 cgtaataaat gaaattagta aaatctttaa acactcggtt cgatgccatg aggttgatag
    20401 aataattaaa ttcgtaaacg cctatagcgt atacatcggg tggaagcgat acccctccat
    20461 ttggaaagtg caatgtgata gtcgtttcgc ccaaagtatc gtttgctgta aaaaaatact
    20521 tatcgctttc ggcaattaag atagtgtcag gatattgagt tgcactatct atacgataaa
    20581 taaaagcctg agtgctgtcg ccaaaatcgg gtgcatttaa ttttatagtt actgctgtca
    20641 ttacatcagt tttattcaaa acatacttct gtaccatttt gctatgctga cctggtgcat
    20701 ttactccttg taatgaagta gcaggcatgt agatggtata atcgtgtgca aatgttgaat
    20761 caaccacatc gaaaaaagca aaggccgaat cgttaagcac gttttcgtca acaaagtttt
    20821 ttatcattgt accacaatgc acatgatagg tgcctttggc aggcgcaaca aacgcgccta
    20881 tttcataatt caaaaaattg cccgtggtaa ttccggtaaa gtcaatcatg atagtagtat
    20941 catgtaatgt aatcatattg tcgtccatta ccattaggtg cacagttaca gtatcgaaat
    21001 ccatgatacc ataattataa atacggccac caaaagatac agtgctgtta tgatacattg
    21061 gccattctga tagtccattt gttggctcta gttgttttga aataaccaat gaatcgaaac
    21121 cggcatcgta gttaaaagtt tgtgcaaatg tggtgctaat agacgcaagc ataacatata
    21181 gcagtgtgct caagaataat tttttcattt tgtctttttt gattttgagg tgcgaattta
    21241 atgcttttgc tgccactact tttacataat tcagattttt attaacatga ttaagagcag
    21301 gacaacaaat tggcaattga cacatgcaac aaaaagtatt ttgtgatata gctttaagaa
    21361 taaagggtta acttatattt tagtattttt aaacaaccaa aaaggtacaa gattttaact
    21421 aatttcaaat tggtatcacc tttctttctt agtgtttctg tgtgcattgg tggtttgatg
    21481 gaagcgaatt tattcaaccc ttaatttaaa ttaatatagc ttgcatgtaa atgagacttg
    21541 ctccatgttt tttaactaat aaaatgagta ctatttggcg cacgaaaaat acttcttgct
    21601 ttatgcaata aaaggcaaga tttttgccaa cccgcaaatt caacaacaaa gggattaagt
    21661 atggctaagg tttggcaggt gattaaaagc actttgattt ttttattcga ttgcatttcg
    21721 ttacgaaaag ttttcaaaaa caagttttta ctttggggtg caaggttcat attgtggact
    21781 gcactgtatg ttggagccat ggaaaccaat tttttgtggt tatgcggcta caccccgact
    21841 ctggaagaaa ttcgccatcc cgatgtagca attgcatcgg agttgataag caccgactcg
    21901 gttattattg gcaaatacta ttacgaaaac cgcacaccta taacgtacga caaaatatcg
    21961 cctttgcttg ttgatgcact tgtagcaaca gaagatgtac gtttttacaa acatcacggg
    22021 ctcgatattt acgctttaat aagcagcatg gtaagcactg cacaaggcga tcaacgtggt
    22081 ggaagtacta ttacacagca gttggtaaaa aacatgttca agacccgcaa agaaaccaat
    22141 caaggattgc tgcaatatat tcctattgta aaaaccgtaa tttataaaag taaagagtgg
    22201 atcactgcaa ccaagcttga gctgttttac agcaagcagg atattttgga gatgtatttc
    22261 aacacagtcg attttggtaa caattggttt ggggtaaagg tggcatcgca aaattatttt
    22321 agtaagcaac ccctcgagtt gaaacttgaa gaagctgcat tgcttgtagg catgctgaaa
    22381 gccacaagta gctataaccc cataagaaat aaaaagcgaa gcaccgagcg taggaatgta
    22441 gtgctggggc aaatgctgaa gtacgattac atcaaacaaa acacatttga tagtgtatcg
    22501 gcattgccaa taaaattaac gcttaagcaa aaaaaacgca gcgatgaaca cgattcgtat
    22561 ataagagtgc aggctgaaaa tattttgaaa aagtggtgtg aaaagtatgg gtacaatctt
    22621 tacgaagacg gtttgaaaat atattgcacc atcgattcgc gtttgcaaaa atttgccgaa
    22681 gatgctacac gcgaccacat gtctaaatta caatcgcagt tttataaaca atggggcaaa
    22741 caaaatccct ggcgcaatga aaatggtggc gagattgcga attttataga agacaatata
    22801 aaaaacaccc ccgtatatgc tgacctagtg cggcagtatg gcgaaaaatc ggacagtgtg
    22861 tggaaaatgt tgcgtgcccc aaaacaaatg aaagtattta cgtggtcggg tgctaaagac
    22921 actacatttt catcgtatga ctcgttgcgg tactatgcaa aaattttacg ctccggcatg
    22981 atgtcgctcg acccatacac aggttatata aaggcttatg taggcggtat cgatcacaag
    23041 ttttttaaat tcgatcaggt atttcagggc aagcgccaac cgggcagcac atttaaaccc
    23101 ttcgcttatt tagctgctat ggattgtggt tttagtcctt gcagccgtat gaccgacaag
    23161 ccaataaaaa taagatacga aggcgggcaa gagtgggaac cacacaatgc aaatgaaaaa
    23221 ttcagctacc gcaatatgac attgcgcagg gctttgggtc gttcatgtaa tgccataact
    23281 gcacaactta ccgatagtat tggatggggc gaggtgattg aatatgccca caaatgcggc
    23341 attcgcagca agttagattc agtgccttcc atttgtttgg gctgcagcga tgtaagcttg
    23401 ttcgagttgg taaatgcata tggtacaatt ctcaatcaag gcatacgcgt tgatcctatg
    23461 ctgattgtaa aaataaccga caaaaacggg aaaatgctgg ctgagtttaa gccacaattt
    23521 gaacagatgg ttaatcccga atcggcatgg ctaacattat ttatgctcat gggttccata
    23581 caagaaccgg gtggcacagc acaaagtatg tggggctaca aagtttttga tgatggcaat
    23641 gaaatagcaa gtaaaaccgg tactaccagt aacgaaagcg atggttggtt tgtgggcatt
    23701 acaaaaaatc ttgtaacagg tgcatgggta ggtgccgatt accgcagtgt acatttacgc
    23761 tatgggcttg ggcaaggcgc ccacagtgca ttgcctattg ttggcaagta tctcgaaaaa
    23821 gcactacata gtaaaggtac aggagtacga acaggaaagc ttagcaaacc accggtaaaa
    23881 atacgtaaag aatactggtg tcaacaacaa gacgatattt atttgcgcga tagcataaga
    23941 agcgacagca ccataatagc tccctccgac tctaacttaa ttttcgaaat agaagtgcca
    24001 aaggaattac ttaatgattc ggtgaggtag cgtgtggttt actattcaat cacaataaaa
    24061 tcaatgctga tattagcagt tgtgatttct atttttccga ttctttgttg ctttataaat
    24121 cgtttataaa ctatgtactt tttatccgca aatagacagt aggatttacg tgactagtga
    24181 taatgggaat gagcagatgt ccactttggc aaaaattcca tccatccatt ttcgtttttt
    24241 tataggaaca gccaatttca ataccgtact atttcctttt tttgtcatcc aggctccaac
    24301 tgcatagcag ttgagtctta atgtctgtaa gcgttgatgt ttagcctttt cggagctaac
    24361 ctgcctgaac aagctgatga tattgtaagc taaatttacc atacgcaagg ctgcttctgt
    24421 tgcaaaaaaa tctttcatat taaaaccctc taatgcaaaa tcatatttga gttctttaat
    24481 cctattttcg caatcacctc ttcctttgta ttgctcccaa atttgtgttg caggtagcgt
    24541 ttgatttgta taaaaagtat gatatcggta ttgttcataa tattccatat catcaaacag
    24601 ctttagtttt ttacctgttg ctttgggcct taaatcaacg ctttgtctta taacaataac
    24661 cctgcgttct gtttcccatt ttgatgcctt ataccttgtt tcgctaatcc aaattcctaa
    24721 tccgatagct gtccaggttt tttgttgctt tatcttatct tgtaatggat aatgcattct
    24781 ccccgctatc acgtatggaa tttgtttttg ttctaaatgt ctaaaaatta aatcgcttgc
    24841 aaaaccagaa tcggcacgca gcaaaccaat cgttttgttt ttgagtattg acaatgtttc
    24901 gtttaaaaat tcattacact gatgtgcact tgaggtatcg ccacttctaa gccaactgtt
    24961 tacaaccatg cgctcgttgc ccaaaaaagc aaataatgga tgatgggagg ctctgcctct
    25021 tttggttgga ttatagcctt tatgattgcc ctcctgcaaa tcaccatacc gagtaatgac
    25081 ggtagaatcc aagtctaatg cgtaattatt aaactgcaat tgattaaaaa accattcttg
    25141 caaaaccgga aacacctcat tatttatgcc ttgattaaac ttttgaaaaa atcgtttgta
    25201 ggtcgtatca cttggtattt gtttccatcc aaatatcttt tttaacgtat catctaatcg
    25261 taaaacatgt gtgtgcgaaa atttgtaaca gcctaaccat actgacaata gaaaactttc
    25321 aatgatttcc tgattctcaa acctgttatt gctcttgctt tcgggtatgg gtagattttg
    25381 caatacactg tttaaaccaa ttttatcatt gaattttttc attaacgaaa accctcccca
    25441 tgcacttaca ttatcatcag aatattcaat tttataactc atttttattc gtaagttttg
    25501 gttacaaggt cgaattcttt atgagcattg tcaactaagc atttccaaag tttttaacat
    25561 gttgattgcc tatttgcggt ttattagtca atcaatttat gagttttcat acccaacatt
    25621 ttatgacaaa tattatccgg atttaaaact gcaacgaagt tttattttac aaaattgaaa
    25681 acaccaatgc cttcttgtgt ttcaaatttt acaacatatg gaccactact ccattcatta
    25741 acaccaatta ctgtttttcc aattgcaagt tgtttcttta ttaagcattg acccatataa
    25801 ttcgaaatgg aaagtgctac ctgtttatcg ttattattta tcaccaatgt gccatttgtt
    25861 aaatcataaa taatagaagg atattttaaa tgcatcaaat tttgctgatt taaatttgtt
    25921 gtaattccta caacattact tggcgattga catccggcag tatcggtaat aagacaatag
    25981 taattgcctg caacggatgg aacataaaaa gaatcggtac tgagcacaac attagtgtta
    26041 ttcacattat accattgata cgctgtagca ttactgcaca ataatgtatc gcccgagacc
    26101 agtactaccg gaataggagg ctgtggcaat tcggtaataa ataagggcat aattagcgta
    26161 tcgcaaccta cactgttgca tgctattaaa ataacatcaa agctacccgg gctagcatag
    26221 tatatgttga cagggttttg catagtactt atacttggcg aagccccgga aaaaaaccac
    26281 agccattgtg taggattatt ggtgctgaga tcataaaaat ctatagcctg cttatcgcaa
    26341 aatacagaat cggaagatgc aatattgcaa atgggtaaaa gtaatgggat gcaaaatgac
    26401 tgttcaatta atgcttggtt attgttagca gttatgaatg taccgtacga caaaataggg
    26461 ctattgggcg gactataatt actcgatgtg aaactgtaat tgtaaaccag aatagatatt
    26521 ggtgatgtaa aacaattttg agaagtattg ccattagtat cacatcttat caataacaga
    26581 ttactgtcgc ctacaccaaa gccttcgcca aaacctgata aaacatagcc atttgaatta
    26641 aatgaggtgc agcctgtact tttgcaaaga agtgaatcac cgtaccagtt tgcccataaa
    26701 atatttaagt tggaatcaag ttttatcatt cccattttgg aatcattatt taagtaatca
    26761 tacacataag ttataatgga atggtccgaa ttttcgatac cgtttctaag aataacagtt
    26821 ttgccattaa tgataaatgc tgtgcttgct ataatattta aagcagagtc gagtatccca
    26881 ataagacaat ttttatatcc acccacagtg tttgttccac cagtaactaa atatctgtaa
    26941 gtactcattt tatttatgga taataatgca tcgtcaccgg ggttgccaaa atcgaaaccc
    27001 gaaataaaaa cgccatcttt tgtgagacga taaataatat tgtcgtgtaa accagcgcca
    27061 acataataag cccagcctac cgctataata tcaccattaa acccttccac aatcatgtgt
    27121 gtaacatcat tgtttgatgt gccgaatact ttttgccata acaaattgcc attttgatcg
    27181 cacttaaaaa ccaagttgtt tgagaatcca ttgttgtaac taatgccacc aattacaaaa
    27241 ttgctatcag cggtttgaat tacatgcagt gcctcatctc tttctgggtt gccaattatt
    27301 ttaatccaag taacatttag tgcagaatcg aattttgcta tcatggcatc tatatcacca
    27361 aagccattgt ccttcgtata tccacaagca acaaaacctc catcgaaagt ttgaacaata
    27421 catttaaaca aatctgcatt gggcgagtta ataattcttg tgtttaaagc atttccattt
    27481 gtatctatca gcattgcaat ggcatcgctc ggtgagttaa gactcgaact tgatgcgcca
    27541 cacaaaacaa tgttaccctc tttggtggta tctatatgtg cccattcatc attccctgcc
    27601 aaaccaattc tttttataaa ggtagtttgt gcatggcaag ttcttaaaca aaagagaatg
    27661 gaaaataaaa ataataatct catttactta tactatcatg tttaattgat aagtcaaatg
    27721 taatatatta atttatgatt atatatttta aaatctctcg aagccggtgc cgatggaaaa
    27781 aaatacaaac cctatatcaa actattcaca aaaaagatga tgcgtgattt gctatcaaaa
    27841 tttcaaattg acgcgctcga tatacgccac cttaatagag aagatattcc tttgcttggt
    27901 aaactattta ccgatggcat gttgcaaaaa tacagcaccc ggtatggatg gtacatttta
    27961 gcaaggtgca caaaaaaata aactattttt tgttccgcgt tgcgaggtag aagcctgtag
    28021 ctatggaggg ccacctctca ccaagttttc gggtgagtgc cattattggt tttacaccac
    28081 taaaaagcat atcgctaaat ccacctgaaa aaaatacgga accccgtgtt tcaaatactt
    28141 caaaattatg cttgccacac aatgccttta ttgaattgaa agtaaacctt cgaacatgag
    28201 gtgaatgtgc aatggtcgaa ggatatttac taacgtatgt atctgttttc aatctgatat
    28261 aattaaatgc ctgagaaatg taaagaaatc tgaaaaccct gcggaaattc aaatgcgccc
    28321 aaaatatttc ttcaaattca tgccaaccaa aaccattagg cacggttact atcataattc
    28381 cttcgggttt caatttcgac tttagtaatt ctacaatagg gtcaatatcg cttttttcaa
    28441 tatgctcaaa tacttctgat gcaagaatat aatcgaagtg attatcggga aaagtgttga
    28501 ggtctttaca aaataatcga tcctgtggca aaccatgctc tgcaaatatt ttgttgccca
    28561 aatcgataga ggctttgtct aaatcaatac caatgcattt gtagccatct tgcgtaagtt
    28621 gcgatgtaat cataatgcct gtgccacaac caaactctaa accaagttgc tcttttgtaa
    28681 taaatttttt aaaccatgta aggcgatatt tatgccccca aatgttttcg ttaattttta
    28741 tcatggtgca attttaactg atatttgttt tcaaaaaaaa caagaatgta aataataaaa
    28801 taagatacaa cagagaaaaa agcggctcct tcaataccat attctttaat aaagaaaaag
    28861 tttaaaatga tattggaaaa tgaggctatg aaccagtata aaattaggcg actgggaaca
    28921 ccaattgaat taagaaattg tacattgaca ctttgcacag aaaggaaaaa atttgcaacc
    28981 aataacaatc taaaaacgtt aacgcctaaa atggcattgc ttttaatgaa aatgctcaaa
    29041 ataaattcag caaaaacaaa agcaacagag taaatgatta gcaatactac tgacgctatg
    29101 aatgtctgcc tgcgactaac caagaaccgc tctcggacac cttcaatccg tattaaacga
    29161 ggcaaaagca aagtagacat tacagaggct ataaaaccta gattttcaac aacaagagct
    29221 gatgttgcat ataatcccaa ttgcttatta tctaaaaacc ttttaatgaa aaatacatca
    29281 gctttcataa ttaaaaaccc tagtaggctc aaaaaataaa ttcgtactcc gtatttcaag
    29341 ttatacttaa agtaaggaaa atcaaatctc aaatttacaa tccactcccg ctgtacaaaa
    29401 gtaaatacag ctatggttaa caatggttcg agcaaataat acacgacaca aacatgtaag
    29461 tcaattttat ttagtacgaa aaggattacg attgctacta aactgaagga tttaatgaca
    29521 ctttcaataa tgtttacttt atttattttg ttatcaccaa tcaatatatt tttaaaaagc
    29581 agtaatgaga gatttgcaaa aactgaaaag ctaatgataa ataattcgaa gttggtgaat
    29641 gattttatta ttccggcatt ttgaaaaatt gcaatacaaa gtatagttaa taggagaaca
    29701 aataatgcaa accaaaatga atttactaag agtttcgggc aattttgtgg ttcctttgaa
    29761 gcatgaaaaa tattggcgtt tacgatacct aaatttgaaa atacaacaaa caatgccaca
    29821 gttgccagta taaatgaaag ctgccctcta tcatctaaat taagagaacg tgtaataaga
    29881 atactacata ccaatgaaga aatcaaagct atgattcttg aaaaaatgct ttttaataaa
    29941 ttttgaacat tagcactttg caagatttga aaaaaagttt cgctattaat tttttgcctg
    30001 atagaattca ttttgagcaa agcaaaaaaa ataaatcgaa ttcaataaat ttattcaaac
    30061 aatcaagttg tttttaatat atttgacata aataattttt cattgtcaat gccatcaaca
    30121 acattaaaga atactatttg tttaacctgt tttgtggttt tgtcatatat tctcttaggt
    30181 caaaacaact ataataattg gcattttgga aggtatgctg gagtttcatt caacacaata
    30241 attccaactg cgattacaaa tagtgctatg actcaattgg aaggctgcgc ttctgaaagc
    30301 gatccaataa caggtgctct cagattttac actaatggaa taagtgtttg gaattccaat
    30361 aatactgtga tgccgaatgg cacaaattta tttggcgctg cttcttctgc gcaaagcgcc
    30421 ttaattgtcc caatgccttc tgatacaagc aagttctaca tatttactgt ttccaattac
    30481 tttacaaacg gcagcccagg tgctcattat tccttagttg atatgaattt aaatggaggc
    30541 ttaggagatg taacaactaa aaacgtattg ttgcataacc caacttcaga aaaacttgta
    30601 gcaacacatc aggcaaacaa ttttgattat tgggtactaa cccattcatt taatggcact
    30661 acgttttaca cataccccgt gacaaactct ggagtagggg tgcctataat ctcaacttgc
    30721 agcccaaatt tcggaggaac atcaagtatc ggttgtatga aattgtcacc tgatggaagt
    30781 cagcttgcgg tggcacgttc agggtcaaat gcaatagaaa tatataattt cgaccgctat
    30841 actggtatcg tctccggacc agctttcacg attagttcaa tcgctcccaa cggactggtg
    30901 tatggtgttg ctttttcgcc aaatgggaaa attctttact caactgtgaa cgataacggt
    30961 tttgtttatc aattcgattt atcttccggt aatcaagcca caataacagc aagcaagact
    31021 attgttggca atgcacctgg ttcgcaatat tttcgttcta tggcaatgca actaggtcca
    31081 gataaaaagc tctatattgt aagcgacatg gatcattaca ttgcagccat tagtgatcct
    31141 gatattgttg gtaacggttg caattataat ccatatgcag tgtaccttac ctcctccaaa
    31201 acaggtgcac tcggcttgcc taattgtatt gaaacaatat taataagaaa aatttgtgct
    31261 tttaatcttt gtgccgggga ttctgcaata ttcaattcat tttctactgc gggtttatct
    31321 caatcctatt ggaattttgg tgacacactt tcaaattcaa atattgattc aggtttttcc
    31381 gcttctcatt tttatggatc gccaggaagc tacactgttc agcagattaa ggttcatgca
    31441 aatgggataa tcgacaccat aattcaacaa atcactattc attataaacc atatctaatt
    31501 ggcagtgacg acaccacttt gtgctttggc caacaactca ctttaactcc agtaaccaat
    31561 gcaacaaact atacttggca aaacggaagt acactttctt catttactgt ctcgttagcc
    31621 ggtacgtata tagttacagt aacaaacact gatggatgca aagcaactga tactactgtt
    31681 attaattaca tttcttgcgg ctcacccccc atcgtctcat tctactcaag cgacactatt
    31741 ctatgcgaaa aaagtgcgat agattttttt gatataagca ccaacaatcc tacacagtgg
    31801 caatggtcgt ttccgggtgc gagtccatca tcgtctactg tacaaaatcc tacaggaatt
    31861 tattatccaa cttatggtgc ttatactgta acacttgttg cgaccaatgc taatggcagc
    31921 gactcgctaa cgataaccca gtatatcact gtggttgcaa accccccggc accatcggtt
    31981 acagtaaacg gtagctttat gtgttgtaat aatgttgccg tttggtatca atggtattat
    32041 aataatgttg caattcccaa tgcaaatgca atgtgctata atgcaatgca gcccggcaat
    32101 tattatgtgt tgattacgga cagtaatggc tgcaactcgg gcagtgggca aatagtaatc
    32161 tcatcgctaa atgaaaccaa tgttgggcca aattttaatg catggtatga tgctacttct
    32221 gggaatgtaa atgttgttat gactaaaagt aagaatgaga aagcaaccgt aactatatac
    32281 gatgtgttag ggcaaactat taaacaaata gaagaagaaa attctgagaa taataaaatc
    32341 atgatcatcc cggcacaatt tattgcaaaa ggaatataca tgatagaagt tgcaacaaag
    32401 caaaaaacca gctccggcaa gttgctcata cattaattct cctaaaccat gctttggagc
    32461 acaaaaatta attttcaaca aatgtaaatc gcttatggcg atgctattac tttacatctt
    32521 aaaacaaatt aaaaacaaga ccaatgtcag taaattcgaa acatttagcc acatttctgt
    32581 taggtgccgc ggcagcactt gcagcaaaaa aatacatgga catgactccc gaagaaaagg
    32641 agaaaatggc tgccgacctt aaagctaaag ccaataatat gaaagatgaa gccgaggcca
    32701 tgatggaaaa agccaatact tatttcgaag aattgaagtc caaaggtagc gaggccatta
    32761 aagaacattt tccggaggca gaaaacttta tgaaagattt ttttgaagga aaaaagaagg
    32821 aagatacgcc tccggtggtt taggtattgg gtattttgtt accaggcatt ggggatagtt
    32881 attaagttgt tgagtatggt ctttggtgac agacattatg ttattttatt tttggttgtt
    32941 gagcatgatg tcgggacatt tgaagaatag cggagacgat atacttcatt atgggaaaat
    33001 cataattcat ttttaagttc ttgcattttt agtgttttgg cagcaacaat cacttgcatt
    33061 tatttgaaat ttattcgtgc atattccaag gtattgggtt ggctgtcaaa aaaatatcgg
    33121 ggaggaaatt agaaaaagaa gccgcagcaa tttccttttc aaattattaa taattattcc
    33181 acttcgaaaa gctgttattg caataaaaac actaaggcac aaaatttcac taaatggtac
    33241 acaaatcatt tttaatattt tgtgggttta agtgttttgg tgatttaatg gcctaaatac
    33301 tgactttccc atgttactca aaaatatttg ttttcgaatc aattaattac ttatctttca
    33361 cacttgaaat ttgtaataca aatcgatttg aatataagct accctcattt tcaatcttta
    33421 accataaccg tacacgatgc aaaaaagcat ttgagtgttg gcagtgttgt ggaaatttgc
    33481 attgggtata ttttaaaaat gttttttgtt tcgaaacagt tgacaaactt gtgttggcgt
    33541 aatttttcag cactcacgct tggttttaga tttttgcaaa aagccccttt acttttaatt
    33601 gcacactcat gaaaattaaa ttcctcatac ttgtttttgc tttaaactgc tggcagtttt
    33661 gcttcgggca acaaggaatg tggacgtggg taaatggcac atcggtgggt aaccagaatg
    33721 gagtatatgg tacacaaggc gtggccgacc ccaacaacac ccctcccggt acttatgaag
    33781 ctacagaatg gaccgatcat gatggaaact tctggatgtt tggcggtagt cttaatagca
    33841 gtaccattta tgccgatttg tggaagtaca accctaacaa caatatgtgg acatgggtga
    33901 aaggaaatgg cttagccaat gatgcaggcg attatggcat aatgggtgtt gcatcgccca
    33961 gcaaccgccc tcctgctatg tcttacggtt cggcatcgtg ggtcgacagc aaaggcgacc
    34021 tttggatgta cggtggttta gttgtgggct gcgttcgcaa tcacttatgg aaatacaaca
    34081 tagccaccaa tatgtggacc tggataaatg gttcttcggg atgtaacaat gtagcggttt
    34141 atggaatttt gggcgttcca tcttcactta atactcccgg tgcccgcagc gaattcagtg
    34201 cttcgtggat agatgaagat gataacctgt ggttgtttgg tggaaataac ctaaacgatt
    34261 tatggcgata caataccgca acaaatgagt ggacatggat gaaaggtggc aaccttcccg
    34321 gtcaggtaaa tatgcacggt attaaaggtg taccaaatag ttcgaacaca ccgggctcac
    34381 gttttgcatt tgctacatgg aaagatttga acaaaaaatt ctggctcttt ggcgggcata
    34441 attatgtgcc gggacattca tacaatgata tgtggcaata tgatcctctg accaatatat
    34501 ggacttggat gagtggaacc aatattccaa atgatgccgg aaactatgga ccgttgtgct
    34561 ctccatcgat aaataacata cctgctgcgg gttatgaaaa tcgcgcacgc tggactgatg
    34621 aatgtggcaa tttttggatg tacagcggtg ccggggcata tctaaacgac ttgtggcaat
    34681 ttaatcctac tactttggaa tgggcattta ttaatgggca aagcattaca tggttgcaac
    34741 cacctgtata tggaatacgc aataccccgg caatttccaa cacacccgga acacgcaccg
    34801 gagtgattgg atggcgcagc actacaggcg aattattttt gtttggtggt atagtaaatg
    34861 ttaccgacag aaataatgat atgtggcgtt ttgtgcccga taccgcttgc atgggttgta
    34921 agtctaactt ctatcctgag gtgtattttg catcgagcga tacagtgctt tgcgaaaaaa
    34981 gctgcatcga ttttaccgac ctttctttaa acaatcccat acaatggcaa tggcatttta
    35041 atggtggcaa tcctaattcg tcaacaatac aaaatcctac aaatatatgt ttcaatgctt
    35101 atggcgattt tgtaataact ttggttgcaa caaatgcaat tggaaccgat tcggcagttt
    35161 ataattcatt cattcatgtg gtgcaaagcc cgcctcagcc aactatttcc ataaataagg
    35221 atacgctttt ttcttcgcca gctgtaactt atcaatggta cttcaataac gttttgattc
    35281 cgggagcaac tttaccgtat tatgtggtgc aggcaccggg aaattattat gttatagcaa
    35341 cagatacaaa tggttgtggc aataattcaa tacaggtgat tataaatagt gtgaatatta
    35401 gtgaagagca aaatagcgga attcatattt atccaaaccc ggcaaatgat gaaattgaaa
    35461 ttttgtttga ttcaaaaata aataccagtt caattttaat aatctatgat cctattggaa
    35521 aaaaggtatt tgaaaaaact attaccaata caaatagatt gaacattgat gtggcaaagt
    35581 ttgaaaacgg cttgtatata ttagaactga acgataaatc gaattttatc cacacctctt
    35641 ttttggtgca acattaatat tgcttttgcg cttcaataaa attattcttc ccgatccttt
    35701 taagaatttc aattgctaaa aaaaatcgaa actattttgc cggagaagga ttcgactcac
    35761 gcacaatttt cttgttagga tcgggcaatc gcatttctac taatttgttt gtacaataaa
    35821 cgcactttcc actatcagca ggcacagtgc cacagtggat acaatagaag tcgcccactt
    35881 tacaagtttt catttgacaa aaagtacaat tgccggcagc atttgccacc aaactacaat
    35941 taggacatac atacttatct gaagtttttt ttgcagtttg cttttgcgat tctactttgg
    36001 atgattgagc aaaaacaaat tgtgatgtgg cgaagcaaat cagaacaaac gttaggagag
    36061 ttttgaattt cattttataa aatttttctc aaaaatacat gattgaatat tgtgtttaac
    36121 gaaattgaaa tgaatttctt ttcaatttta tattgctttc acttcaaaag gcatatattg
    36181 gaatgcaacc gttttgatgt gcctttgcgc catagcaatc atttagagcg attaatacag
    36241 gtacattaaa acaaaaatgt ttggttctac tttggcctta aaataaaata aacaatatga
    36301 tttataaaag agaaaatgag aagaataaat cgtagatatt aaatcattaa actatattag
    36361 cgttcacatt ttaaaacaca gcatcgaatg aaaaaaaata ttctttactc catcgtgttg
    36421 attggtatat taaccaacat ttgctttgca caaaaggagg caacaaaatg gtattttggc
    36481 aacaatgccg ggctcgattt tataagtggt gttcctgttc ctttttcggg tagcgctatg
    36541 acaatgcttg aaggcgtggc atctattgcc gacactgccg gtaatacatt gttctacaca
    36601 aatggttttc aggtttggaa taaaaataat gtggtgatgc caaatggtaa cggtttgtcg
    36661 ggtaacaaca attcggcaca agctgctatc attattccgg ttccaaatag tgcatcgcaa
    36721 tattacattg taactacccc atcaaatgga aacggtacaa tggattattc attggttgat
    36781 atgaatttgc aaggcggact tggtgatgta accaccaaaa atgttccgct attcaataac
    36841 tcaaccgaaa aagttacatc agtacaacat gcaaatggaa ttgatatatg gttgattggt
    36901 catgaatgga atacaaataa tttttatagc tttcaaatta caccggcagg tataaatccg
    36961 gcaccagttg taacatcaat tggtagtgtg cataatggag tactcggctt tgcaggttat
    37021 atgaaggcat cgcacaatgg aacaaaaata gcttatgcta ttactatttc gctcaacatt
    37081 gttgaacttc tcgattttga tattgcaacc ggaattccat ttaatccaat tacttttaca
    37141 aatatacagg aagcatatgg tttagaattt tcgcccgatg attcgaaatt gtatgcaaca
    37201 agcgaaaacc cacaaatact ttatcaatgg gatgtaaatg ccggttcgcc tgctgcgata
    37261 atagcatcgc aattggctat taatgcaagt caaggtaacg aagcctttgc agctttgcaa
    37321 cttgcacccg atggaaaaat ttatttggct aacagattca gtaccatgat gggcgtaata
    37381 aataatccga acgatgccgg ccttgcctgt aattttgttg atgatgtttt ttcgttgcct
    37441 tttggatcaa accaatatgg gttgcccaat ttttttcaaa gttattttga tacaattcca
    37501 gcttctggta ctgctccttt ggtcgcattc caatcgagcg atactgtgct atgcgaaaaa
    37561 agtgctatcg atttttttga tttatcatcg ggctcaccca caaactggaa ttggtatttc
    37621 cccggtgctg tgcccgatac atcaacattg caaaatcctg ccggaattta ttatgccagc
    37681 tatggcacat ttcctgttac actcacggtg tcaaattcct ttgggcaaga ttcacttacc
    37741 attttatcct ttatcactgt ggttccaaat cctacgcaac ctgtaattac acaaacgggc
    37801 aacgatttgt gtgctccacc ttttatttac tatcaatggt tctataatgg cacaactata
    37861 cctcaagcca ccaatcagtg ttatacacca acgcagccgg gcagttatta tgttttggta
    37921 actgatacta atggatgtca gggcgcttcg ttaagtgttg tgattacaaa tttagaaaac
    37981 gctaactctc cctttggatc ggtaagtatt actccaaatc ctgtaacgaa taattttata
    38041 cttactttat cgggcactta ttttgaaaca ttgaatttgg aaataagtga tgcccaagga
    38101 aaaaaagtgt atgacgaaat aattgtgaaa gacactccta ccctttccaa aaccatttcg
    38161 accaaattaa gaagtggcat gtattttata aagattcaaa gtggttcaaa atttatagta
    38221 aagaaatttg tagtaacaga atagtgattt ttacacggat aacttttact ttaagcagat
    38281 gaataatttg aataccgctt gtgctgaaaa gttatttaga aatatgcctt acacttctcg
    38341 tgattatatc cactcatttt gatttaagaa attaaattat tgagtaatat tattttgcta
    38401 tttttacata cgaatcaaat taagatttat aataaaacac atagactatg aagaacaaaa
    38461 tcctacttac atttttaact atcactgtat acgcttgtaa tttattagcg caaccgggtg
    38521 catggacctg gctcaaggga gacccaaatt taaattctgc attccttgga aacaatggta
    38581 tacaaggtgt gccggctgca accaatcaac caccattttt ttatgaagcc cccacattaa
    38641 tggacacttt gggcaattta tttcttatag gtggtggcaa tggaaatggg caatggagcg
    38701 atgtttggcg atataatact gctaccaatg aatggacctg gatgaagggc acaggaatgg
    38761 gaaatgcaac cggagtatat ggagtaaaag gtgtatcgtc acccaccaac caacctgctg
    38821 atatatcatt gggcagcagt tatgacaata ccaaaagtgg catattttat atttatggcg
    38881 gctctgccag tatctcaaat aatttgtggc ttttcaatcc ttacattaat gaatggacat
    38941 ggcttaacgg aaatggtaat ggcggattta ccacacccgt ttatggcgta atgggtgttc
    39001 cttctcctgc caatagcccc ggtggacgaa cagaaggttt gccttcgtgg tgcgatagtg
    39061 ttggcaattt ttggatttat ggcggcagtg gtggtggcac atacggtgac ttgtggaagt
    39121 gggatgtatc aataagtcaa tgggcatgga tgggcggatc ttcgaatagt ttacctgcca
    39181 actacggaac caaaaatgta gcagcagcca ccaactatcc aggtgcgcga tcattttgct
    39241 ctgcatggaa attacacaac aatgaactgt ggcttggcaa tggtgccaat agttcgggta
    39301 actctattaa tgatatctgg aaatatgata tcaatacaaa tttatgggta tgggaaggcg
    39361 gatcttcgct gggatcacca aatcaaaatt taggcacaaa atgcaaccct gctccaagtg
    39421 taatgcctaa tccttcgagc gaaataaaag catgctggac tttttgcgat aatctttatg
    39481 ctattcatag tagttacaat tatacaactg cattcaatac tgtgaccaag aattttacat
    39541 ggataagcgg cagccaaatc cctaatgccc cgggaattta tggcacactc ggagtgccat
    39601 cggttaacaa tgtatgtgca actaaatttg gcggtgccgc gtggtataaa catccttatg
    39661 cttatgtgta ctctggtatt tatggcggaa ccgacatgat gcggtttgaa cccgatacca
    39721 cttgcggtga ctatcttaat ggatgctcac agccggtttc agtattagcg gtaaatttcg
    39781 ccagtagcga taccattctt tgcgaaaaaa atgctgtaga ctttttcgat ctttcggcag
    39841 gaaatccaac tcaatggcaa tggtattttc cgggtgcttc gcccgatact tctactgccc
    39901 aaaacccaac aggtatatat tatgccagct atggaacata tcctgttacg cttgtagtga
    39961 gcaatggcaa tggtagcgat tcggtaacga ttaatatctt tattcaggta gtgtcccaac
    40021 cagcaaaacc aatagtgacg gtaaacggca atattttgtg tagcagcgcg gccagctatt
    40081 atcagtggta ttataacgga acactcatac cgtttgcaac tgcacagtgt tacactacat
    40141 ctcagcttgg caattatttc gttatagtaa ccgattcgaa tggttgccaa aataccagtg
    40201 atcttgtttt gctaaacaaa gttgcggata atgcaaactc aaatcaaatt cagttaatac
    40261 cgcaacctgc taaagagaat gtatcggtac aaataaccaa caacatttat ggtaaattac
    40321 tattacaggt tacagacctt gccgggagag tgatatatga aaaggagcaa ataaaaaact
    40381 accattactt ggaaactgca atcgattgca aagcctggcc cagtggtacg tatacagtta
    40441 gagttaatct ggatggcact ttttttagca agcgaatggt gaagtattag ataaactctc
    40501 ttggcatatg ctgtccaact tactatcctc aacaggattt attggcaatt ttacttgagg
    40561 tagattgaag agtatcattt tatctaaatt tctacgttac tcagttaatt tacttcaaac
    40621 tcttttccaa atcctcaatt gaattataaa gcttcagcct tttgtttttt tctacaattc
    40681 ctatttgtag caagaaagtg ccgattaagt aatgagattt attattggtc aaaacaacac
    40741 gatagtagcc tggtgaaagc ttactatttt tctttttggc atttggctgc ttccgtagca
    40801 aagcattgcg cagcaacaaa ttgggttgtg ccggcaaatc cttactatca tcattctgag
    40861 caatggtaat ccattcaaaa atattggatg gaagttttga tgtttttaaa tatccttttt
    40921 gaagaattat ggccgggtcg aaatagccct tgtttgtggt agcacactca tagtatgatg
    40981 aatcggtatt gtcacccggc cttggactgg ttatatcggg cacaggagaa attgccctta
    41041 ctcctatttc gattgctgaa tcgggtagat aaaaataaaa cactgttgaa ctgatattgt
    41101 tagaatcaag aaatatagta tcggcaatgc acccataaaa tcccggttgc gaagtataag
    41161 gtatacggtc actgttaata acttgcgatg attcgaattt tgaaagtcgt tgtgctgaag
    41221 tttcaaggca aaaaatcaaa agaagaaaaa tcaaaaaagg tttcattaat atctgtttca
    41281 tcatcagcaa ttgttttata cttcgtaacg tgcatacaac attgagcatg ctaataagcc
    41341 ggcagtttcg gtgcgcaacc ttgcaccacc aaagtgcaca gattcgaaac ctgctgatgt
    41401 ggcctgctca atttcgttaa gactaaaatc cccttcgggt ccaattaata tagttacatc
    41461 atcatgctta tcaatataag aacgataact ttgtctcgga acatcactta tacaatgtgc
    41521 aattagtttg cttttgccgg taagtgattc tataaaacta ttataactta ctaaagaatt
    41581 tattttagga aaggaaacct tttgcgactg cttacaggcg gaaattataa tattttgtag
    41641 tcgtgcgatg tttacatttt ttttaactgt acgtgaacat gccaccagcg aaaagctatc
    41701 gatgcccatt tcaactgcct tttctatcat ccattcaagt ctatccatat ttcgtaatgg
    41761 tgaaatagcc aggtgcaaat tgtaattatg tttagcaaca catgtgtgca atgccaaagg
    41821 aacagcaaca cacttgcggg catctatatt tgtaatctcg gccaatgcat aattagcatt
    41881 gccatcggta acatttattt tatcgcccga atgcaaacgc aaaacttttg ctgcatgaaa
    41941 cgattcggct tcggttaaaa taatttccga atcacataca ttggaagaaa taaaaaaagg
    42001 caaatcggga taagtcataa ttataaaatt acaatcagtg caataattac aaacaggata
    42061 ataaataaca gtgcccacaa atcggcaggg aaaaaattct tatattgatc atatagtttt
    42121 ataaaataat tcttcatgcg gtatgagtga caactatttt gaattggttt tatttttttt
    42181 cttttttgaa atcaactcac gataccttgt aaactcttgc cggtaactta atcctactcc
    42241 ttgtgtatag ggcgcctgat ttgtatttat gagagagtta gcatttgact cattgaacgc
    42301 tttcactcga aaattgccat tcttgctaat tacatattca atatcgaatt cgcccacaat
    42361 gctattattg gcggaatttt cgttgccccc gtttccattt actccaacat ttgtattaag
    42421 tataagacgg tcttgcaaaa gttgtgtcga aacaagtaca ttgtattgtt cactggtgat
    42481 aacatcaccc ggagtgtaac tcaaattgat atcgacattt ttatttattt gagataccca
    42541 atcgctcagc tgtttgctca aaaattcagt taggctcgaa aggccgctat tgccacccgt
    42601 attcacagtt tgcgtacctt gcaacgctga tggcagttga aatttattca gaaaaattat
    42661 actcatcacc tgcttgtttt tttcatcatc ggtatcaatg tatcttttta ttctgctgct
    42721 tgtggcggca tctacgttag gcacttctat atcaaacgtt acatcgggcg taaacaaatt
    42781 atttttcaag tttagcacaa catttacagg cacttttctg gcaaatgcag cagagtcgtc
    42841 aggcaataaa tcggcaagcg aaaccggctt ggtatacaat gcttttatgt caatggtagc
    42901 ctgataagga tcgccattcc aacgaatggt actacctttt acaagtttaa atggtttgcc
    42961 ggcaatattc tgtaaagtaa aaaagtaatc gcctttagaa attgtatact caccaaacat
    43021 attgaaagcg ccatcgggtg tgatttccaa tttcaaattt cctttaccct gccccgcaat
    43081 tacgtcacca actttttcat cgaatatcaa tttgattatt gcatcttcgg taacatccat
    43141 ttcaaaatcg atatcgaaac ctgataaact tgatatcttt tcttttttgt ccaactccac
    43201 tgcaccatgt ttttcgaaag ttattaaatc attttcgctc acttcttcgg gtgtcgacag
    43261 tggtatattt atttgtgtat tcttttcggt tcgcaatttt atttgcatgg cggtaaggtc
    43321 aggtgtacct gttagatcta ctttaccagt tgcgaatgct ttgccataaa acaaatcgtt
    43381 ttggccggca tgcgtattca tcatcaataa attattagca tcaatatgaa tatcgaattt
    43441 tatatcatca aaaaatttgt gatacagttt tccactcacc attcctttat tgccaagcga
    43501 atcgaaaact tcaatattat taaagccaat atacttttct ttaaaatcaa tttcgtcact
    43561 caggctatac cttgtattga ggtagtcgat tgtaaatttt cctttttgaa ccaaggcctt
    43621 cccggtgatg atgggtgttt ttgctgtacc cgatattttt aattttcctg ttcctcgtcc
    43681 tgtaagattc gaaaccagcc catcgaaata atgttgcaat gtgctcagtt gtattttttg
    43741 tacacttaca tcaaagtcaa gataatcgtc cttttctcct attcgataat ctccatcgaa
    43801 agtaagtgta ctatcgccat gccgcattac attaccttcc aaatgtaaaa cttcactctt
    43861 cgagttgtag tcacttacaa tatttgcatc gcctatggag tctttaaaaa atgcaaagtc
    43921 tttgataaat aaattaccat ttaccaaagg tttatcattc aggcccatta agtttacatt
    43981 gccatttagc acaccaccaa attccacatc atatactaca agcagtttgt taagcggacg
    44041 cacatcaaaa tccttaaaaa taaaacgcag tggctctgct gcattaccac caacgttacc
    44101 gttaatggca attttattaa agtaattact ttttaaaacc acattgttaa aagtaaaacc
    44161 attggcatca taaatagtag aattctcatc attaaatttc cagagcattg tatcaacttt
    44221 caccaatgtg cttggctcta tatggatgct tgtaatacca tcgttgcgaa attcagaatt
    44281 gaaagcaaac acaagtttat tggttgcagt atctacatcg tgaatggcca gcttaatata
    44341 tgcactttga ttgtcagaat ttccatcaat agttactttt tcgatgggaa tatttggcgc
    44401 aagaaaaaag ttctgcatat tggttacaaa atcaaacttg cctttattat ctacgccttt
    44461 tattgcaagg ccttcgcagc gcatatcgtt ccatgtaaca agtggtgatg ataaaaataa
    44521 atcgatgtgc tttgtcatgc tatttatttc gcccgataag gcagtgccgg gtgctatatg
    44581 caaaggcgga taaaaaatat taaaaaccgg atcgaggttt tttatattta tttcaaactg
    44641 acagttttcg tcaaatattt ttttctcata gggtttcaac gttggcacgt atgaggtgat
    44701 agtattccat aatgaataat atgcggtaga aagttttttt acttcatcga cagatgcatc
    44761 tatcaaatca cttttcaaac tgatgttata cttgtctttg tattttgttg atacaaaact
    44821 taaactatca atgtctataa ccttaccatc ctgcaaataa ctaacatcgc gcaacagtgc
    44881 aataccattc atgttatcaa tgttgtcgcc ttcaaaatcc atttttacgt taccgccaaa
    44941 ttcaccgttg ccattgcctt tgagcaaatg cagtgcatat ggttttaaat gatctatatc
    45001 agcctcgaaa tcatatatgg gctttgcctg attgaaatct actttgccat taaagaccaa
    45061 accgaggtta tcttcattga catttaaaaa tccattgaat aattttttag ccacgctgcc
    45121 cttaatatca acatttgaat aatcgtattt attgaaatgt aatttcgcaa tattgccttc
    45181 tacttccgca ttcattttgt ttatgtcaaa atattttccc ttcacattca aattgaaagt
    45241 ggtgcgacca agaacgtctt cagcatcgaa caaggtgcca atatcaaact catttgcact
    45301 tacatttcca ctatattttt cgttatcagt atctaattta agattcacat cggccgacaa
    45361 atttcctaat gcagtttcaa tatttccata agtaacaaag tcgtaaatgt aacctgtaaa
    45421 atggccctta aattttaaat caccggcctt ggcaagccat tgaggaattt ctattttttc
    45481 gttttcataa aatggatatt gcggaatggt ttcaatttct ttttggttaa gcacaagctt
    45541 ttttaagtcg gcttcaatat aggtttgttc tatatcgggc aatcccgaca tggctatatt
    45601 gcctgccatt acacatttag ctccataatt tataatcatg ttacggccac gcagcctatc
    45661 gatagtgccc ttgacatcgc cctgcactgt cacaatttta ttcagtccct ttagctcacg
    45721 ggcaaaaaac gccaaatcgt ttgacgataa aatgctggaa gtaaacgatg actgtatacg
    45781 caccttttca ataaactctt caaaatcttc ccactggtcg aataaaaatg caatctgccc
    45841 atgaacatta ctctgtgctg ttttcaatgt caattcatcg caatggagaa tactatcagt
    45901 aaagccaact ttagtattta gcttttcaat agcaaatccc ttttgctcgc gaaacgaaat
    45961 attttctgca ttgaaataaa cagaatcgtg taaataaaag tcactcagct cagcagtgaa
    46021 attatcaact ttaatatcat cgaaatctac cagcggagta aaatcatccc accgataatc
    46081 cttgtatcga aagtctatgt tctctacttt aatattatcc aaagcaacat cccatggttt
    46141 ctttttggag ggtgtagtat cggtatctgc aaaataatcg gtaatgatat caaggttcag
    46201 gcctttacgt tctttaaatc gtttaagatt gaaaagccct tgtgataaag taactttgct
    46261 gatgtgaatt ttttggccat taatactcag caaactgata gctgcatcaa gcttatcaac
    46321 atataacaaa gtatcttgct tttcatcttc tacataaagt tgatggaagc ggaaggtttt
    46381 gaaaaaaaca aaatcgacag agccaatggt aactttggtt tttaattctg cagaaagaaa
    46441 atcagtaatt ttatgcacaa ctgctgtttg caccgatggc aacctaagcg caaaagcaaa
    46501 acctgttagt agcagaagca ctattaacaa cgtccataaa gttattttaa aaacacgttt
    46561 tgccataatt aagaattgcg aaaataggaa ttgccggcaa gaataaaaat gacaaataca
    46621 ttaattagat ttgcaccatg aataacggta agtatatact aggaattgaa tcatcgtgcg
    46681 atgacactgc tgctgctgta tgtctggata atatggtact gagcaatgtt attgccaatc
    46741 aaaaagtaca tgaacaatat ggtggtgtgg tgcccgaatt ggcatcgcgt gcgcatcagg
    46801 ccaacatagt accggtagtg accgaggcat tgaggcaggc aggtatcgaa aaaaagaagc
    46861 ttagcgcaat tgcgtataca cgtgggccgg gccttttggg ttcgctgttg gtaggttcgt
    46921 gttttgcaaa gtcgcttgct caggcattaa acattcccgc tattgaaata aaccacatgc
    46981 aagcgcatat tcttgcacat tttgccatta gaaagcaaag tgatgccaca ccaacatttc
    47041 cgtttttgtg cctcactgta tcgggcggac atacccaaat agtaaaagtt gaatccgcat
    47101 tgcaaatgaa aattatagga caaaccctcg atgatgctgc aggtgaggct ttcgataaaa
    47161 tttcgaaagt tattggcttg ccataccctg gcggaccttt gatagataaa aactcaaaac
    47221 ttggcaaccc acatgcattc aaattcaaca aatcgaatat tgcgaactat gatttcagtt
    47281 ttagtggctt taaaacaaat gtgatgaatt ttattcacaa cgaaacccag aagaatggaa
    47341 attttattga acaaaatcta aatgatattt gtgcatcggt gcagcataca attataaata
    47401 tgctgatgga taaaataatc aaagccgcta tagatttgca aataaaaact gtggtgattg
    47461 ccggtggcgt ttctgccaat agtttgttgc gcagccgttt gcaagaaaca ggcaatgaaa
    47521 tgggttggaa tgtttttata cctccattcg agttttgtac cgacaatgca gcaatgattg
    47581 ccgtggccgg tttttttaaa tataacgaaa atcaattttc cagtttagtg gattgtgcta
    47641 cagcccgata tgagtggtag ttttctttcg acttaaaatt gcccatcttc aaatttgaaa
    47701 acttttagca tcaaaacttc tttcaaattt tacatgtctt tttcttttta aattatgata
    47761 atcttaatat tttgaaaagc gcaaccccac catccaataa atattagaag cataatatcc
    47821 tttgaaaaaa aaatgcaccc tgaggtgcat tttttgtaaa agggcaaatt ttattgctta
    47881 cgaaacaagc actactttct tcttgagctc ttaccctctg cttttttggg aactacccga
    47941 ggcttcgact caggtttcac ttttggcact tccggtgttt gtttctttgg ctctactaca
    48001 ggcttcgatt cctctttctt atcttggcgt ggcgctggtt ctgccttttc attattattc
    48061 cttggtcttg gtgccggttc ggctttatca ttatttcttg gccgcggagc cggttcaata
    48121 ttattgttca catcacgggg attatctctt ggttttatat cttgcgcatc gttgtttttc
    48181 atcggtctgc cttctacagg ctttgcatca tttcgtttgt ttattgttgg aaaatttggc
    48241 ttgccctgag gtgaagcagg agcggcatca gctttttcat aggtacggtt atatacaccg
    48301 ttagctctgt tgcgcactac aatttgactg gtacgatgat ggccataata agtattgcgc
    48361 cagtgattct gaaaatagaa attatttgca ggacgatacc accatccatt ccatgcataa
    48421 tcatagtaac aatggtgatg atagtgatgc caaaacatgg gctgccatgg tctccaccaa
    48481 ccggggtagt agccccaata ccagggcgaa ttccaaacca tgtatccagg ggtgtacaaa
    48541 taatttacaa tgtaccatcc cgaaggatcc gggctgtaac tttgattggt gttgtagtaa
    48601 ttatttgttg tgtaattgtt tgttataggg tcgttgccac tatatccggg gttaggagtt
    48661 tctcgtgctg tattttttgt tgatggttct atcacataat ctttgccata caaatcttca
    48721 tcgccaataa tttgaatacg tgtttggtca ttgcttttgt caacgtatat tacggctaca
    48781 tcctgcacat cgtcctttgc caaatcggct tgtaaaatga tagaatgcaa agtgccatct
    48841 actttgtcaa ctactctcaa ataatctatc tgattatcgt tattcaaatc gaggttgttt
    48901 atcttacttg aggcgctatt cagtgattgt tcaaaagcct caagggtttt cgattgctga
    48961 aaaatgtcga gaaccgctgc aaggtttaag ttatcaccgg gcaaattaag cctgtcattg
    49021 ttgtcctgtg attgtgccac atacccgatg caaaataagc atgcgaatgc aaaatgttta
    49081 atccttaaat gtttcgttat cataattttt acttttaata ttaatagggc aaacttaagt
    49141 cacagatgtt gaaataatta tgaggaatgt cattcataat attttgttca tttttaaatc
    49201 attgctcaca atcggttgca aaatgggttt ggaaaaaata tttccaagta aactgaaatc
    49261 attctttttc ataaccagtt tagcattcac agtcgggaat aggtttttca actcattctt
    49321 actcaactaa acctgcaaca gtttaaaatt ttacatttga ttctcacatc aacgaattac
    49381 gaatttgttc gaatttactt ctgtcatgaa ttcgtaattt cttttttgac ctcaacattt
    49441 tggtcgtgat gccgaccgaa gtgtcagcat tcgaaattcg ctgatagcat ttaagaattt
    49501 taacccggtg ttgtatgata atatgataca tatcacattt caaccaaaac aaaataatta
    49561 tatttcgcgc ttaaaataaa ataccatgct atcaaacaat aagacacagc atatacctac
    49621 agaggaacaa atcgaaatcg acaaaaagtt ttttattctt cagcaaagct ttagccgaaa
    49681 ctatgaatac attttttcga acgacctggc cgaaaaaaca gttatcataa ttcccagtct
    49741 tacccttgac gaagaaatgc ttaaaacaat taaaggcgtg agtcattacg aagagcgcat
    49801 gctatgtatg ctgatgttat tgcgcatgcc caaaacaaat gtggtgtatt tatcgagcgt
    49861 gcctatcgat agcactgtta tagattacta ccttcatttt ttgccgggca ttacaggtta
    49921 ccatgcacgg caaaggttaa cgctgcttag ttgttatgat gcctcgcgaa aatcgcttac
    49981 cgaaaaaatt cttgaccgcc cacggttaat cgaaagaatt ttgcattccg ttcgcaaccc
    50041 agcaatggca catattgctt gtttcaatgt taccaatttc gaaaaggaac tggccgtaaa
    50101 attgaatgtc cctgtatttg gtaccgaccc ggctttgttg cattggggtt caaaaaccgg
    50161 aagcagaatt atttttaaac gtgcgggaat accattatct cccggtttcg aagacttgaa
    50221 agacgaaggt gatattgtaa aagcattgat agatttaaaa aagaataacc ccacgttaaa
    50281 acgggcagtg gtaaaaatga atgatggatt ttcgggcgaa ggcaatgctg tttttaatta
    50341 tggagatatt gatgtgaacg atgcaaatat cacaaacata gttaccgatg aattgcgaaa
    50401 caatttaaaa cttgttgcgc gcaatgtgcc ttaccaatta ttcttagaca aatttataga
    50461 tatgcacggc attgtcgaag aatttgtgga aggcgaaaaa aaggaatcgc cctcggtgca
    50521 aatacgcatt aaccctgcaa gtgaaatcgc catcatttca actcatgacc aaatccttgg
    50581 tggcgaaagc gggcaagttt atttgggggc atcgtttcct gccgacccgg attatagtaa
    50641 agagattgca caacttggaa atttagtagc catcgaacta ttgaaagaag gtatacttgg
    50701 ccggtttgct gttgactttc tctcggtaaa gaatgacgac aaatggttgc attatgctat
    50761 cgaaattaat ttacgcaagg gcggcaccac acatcctttt ttgatgttgc aatttcttac
    50821 cgaaggaact tacgattaca acaatggtgt ataccttatg cccaatggag aagcacgttg
    50881 ctactttgcc agcgacaacc ttgcccgcca tgaatacaaa ggcctcaccc cacacgacct
    50941 gattgatatt gcggtttgca acaatctatt gtacgatagc gtgaagcaat gcggtgtaat
    51001 gtttaacatg ataggtgcgc tatcacaata tggtaaattg ggagtggtat gcattggaag
    51061 aaatgtggaa gaagcaaaag catactacta caaaacagaa catgttttga atttagaaag
    51121 tatgaggaca gcatcgaaaa tttgatgatt caatttttat aaaattgaaa ggcagtttcc
    51181 tttttgaaaa agtcagcaag tatttgttgg tttgcaaata atagaaggcc cgtttgctga
    51241 agggtggcaa aaaaaataat ttgaatttct ttttggagtg gagaaaaaaa tactctctta
    51301 aaaaaattgc ttgcagcatt ggcttgtttt tttttaagtg tgtgtttttt gtgggttggc
    51361 tttttcatca gagtgttaaa cttaattgat tcggattgta ttcacaccaa ttcaaagggt
    51421 ctccatgaat tccttgatga tggttgcttg ttacccaatc aatgaaagca gttactaatt
    51481 catcaggtgt tacaattttt tgtccttgcg ttgtatggca aacatcaagt aactcttttg
    51541 gaattgcagg agccatgttt ccgaaataat agaaatgctc tgaaatcaaa acattctttc
    51601 ctcttgtatc tttccttaaa tgttcagggt tgcaattgcc atttgtcatg caatgagatg
    51661 aatcttcttg aatccaatta ccgcttgcat ccttgtggta aaagttatcg ccaaacattg
    51721 ttactaaagt tccgttcatc accggctttt tatattgaaa tcttacatcg ttccagtaat
    51781 cttcaagtga aattttttca gtaacttgca ttgcgtaaat caattttcct ttcaagcgtt
    51841 tttttgttgc cgcttctata tcaccagaac cagttccaat aatccaatca ccgattgata
    51901 aatttttact cttacgaatt tccgatttac aaactgcaag tgtgcaaaac ttgccaaatg
    51961 gatttggtgc aagtcctaag tcgtgttcta tcaagtatga atacataatc atgattcaac
    52021 actttacttt agttctttga aataatggtg ttgcaacact tccatcagga ttttcagcag
    52081 gaaaattttt gccgtttata ctgtcgctta acttatctaa tgaattccaa ccaatcgctg
    52141 gacttccata ttttttatac gcttcgggta actcaactga attattactt ccgtgagtat
    52201 aaatgccaat tattcttttt ccatacaaat gagccaacct tatttcataa ttcacccatt
    52261 tactgttgtg agtgtcagga ccaattaagc aaacaactgt tgaagacatc ttaacccgaa
    52321 ttctaaggta acgctgaatt accgcatcag atggaattct tccgttttta tgtttcgtgc
    52381 tgtcaattga aaaattgcga acattatttc cttggtcaat taatctttgt ttcaaagatt
    52441 gaacatgaga atcatcttct cccaagtgac ttatgaaaat attattcgct gtgctcattg
    52501 cttatttgat taagtgattg atgttccact tcggtggaaa atgaaattct attgcttctt
    52561 tttctttgtc cgcaaatttt tgtttgattc tcgttctatg tgaatagtcg tctatttcag
    52621 ggtgcgctac tcttgcgtat ttgcctacct cacaaaaaac attttgacaa tctattaact
    52681 gtaaatttct accccacaaa tttttaaagt caagatttaa tctgtcaaat tcctgttctt
    52741 gattttctgc catgaggcga ataatatctt caaatgagta accggcaaaa tccttaaagc
    52801 attttgcaat tccatccttc gctcctgggc ccgcttttac aaattccatt tcactaaaat
    52861 ttgtgagtgt gctgtaattg atgtcggtta catattggta agccaaaaaa cctcctattg
    52921 tcggataaga atgtaaaagg gtgtatgcct cactcatgct tttgcaatgt tgaagtttgt
    52981 aaggcacttt ttcttttatc atcttctcaa ttaaccccaa atgattttga tgtttccttt
    53041 cgtaaccaaa agttgacctg cccgatgcca tgatgtaagc agcagaataa ataggaattc
    53101 tattctgcat cacttccgaa agaaccttgt cataccgttt gaaagaataa tctttgaaac
    53161 tgattttgcc caactcgtat tctaaaactt cccaagttga aatgcgattg aagattttga
    53221 aaagcagcag tcggaaaaaa acttcttcgg gtttctgact gcctttgtaa atcacattct
    53281 taatcaagta ttgacttact ctgtcggttg ctctataaac attcgtgaac ttgtattgat
    53341 taagaatttt gtcattcgtc caagggaaac tttcatcatt gaagcgtttg aaaaaaatat
    53401 tttgtctctc tgtcgcaaac ttccaatagg tgttgtaagc cacacttaat ttaggtttgg
    53461 ctttcttggt cagaatcaaa actggctctt gctttctata ttttgtttct aattctctct
    53521 gcataattat ttagtgaagc ggtttgtaag tcctttcaaa atcaagtaaa ccacttcgct
    53581 tatgtgtggg gtaacagtta atcgtaaatt gtctttttcc gaaagtccgt tttgttctgc
    53641 tattctcttc agtccgattg cttgcaatgt tgaaagatat tttttgtagt cgatggaatt
    53701 gccagaatgc ttttcgttgt aaatcgcaac catgctt
//
LOCUS       DAORQA010000006        52686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_111_length_52686_cov_26.814235, whole genome shotgun sequence.
ACCESSION   DAORQA010000006 DAORQA010000000
VERSION     DAORQA010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 52686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52686
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111_length_52686_cov_26.814235"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..2596
                     /locus_tag="PK736_01185"
     CDS             299..2596
                     /locus_tag="PK736_01185"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRC32036.1"
                     /translation="MTEQFQLWLNKFDFPKIILAKKIWRNIIEKRQQPAMISALLKSD
                     LRSTLSESIITTSFISLADLDFSNLDISTANYLCQKYCTHHFDLLGSGWVSLNYNQKA
                     LGVEGNVYESSFSDIDFDFENNWIAKVLPSSYVQESKKLFDIIRKTNKAYNPIDWHRD
                     FKSGFRFDSQQTHATQYGQIKGEGIDIKVPWELGRLQHLPQLALFAAILPDLKNTIIT
                     EYVCLMYDFMALNPAGIGVNWSCTMDVGIRAANMLVAYDIFCQLDSGNLIDETFKQHF
                     ANYVYSHGYFITNHLEYQEGMGSNHYLSNVAGLLFVASYLQSNEVNDNWWWLSVQELL
                     FETEKQFFSEGSNFEGSTSYHRLSGELITYCSALVCGLGTLRWHQLQKHTQKQLQKEP
                     FINFKFLNNVKKDLVAVYKSGRVEGTIGGNLQMVYKAALFSKNITKHNGEITQIGDND
                     SGRFFKFSPCGKMISNHNAQEKYENLKGYLTMYGNGDFWDENNLNHQTYLSATNGIFE
                     QRILDQSQNLFPFENSLVKSIISKNNQGRKLIFYADFFGQDQTLLNKNHPANLKLDEK
                     NLVHKQSKVFGPYACGDTLFNNLKIITFNEFGLVIVKTDHLFLSIACGTNKNYIHSWG
                     HTHSDKLSIELQVDGVDILLDPGTYIYTPLPNARNAFRAVANHNTLVVNNEEQNWWYD
                     GKKGLFKMMKQTKCEMLNIGSNEIKISLQYRQIIQIRQLLISGNKLIVNDASNKPFVS
                     NWNKLFYSNGYGKKINPGNMLASND"
     gene            2861..9622
                     /locus_tag="PK736_01190"
     CDS             2861..9622
                     /locus_tag="PK736_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32037.1"
                     /translation="MKKIFPVFTILVVIFTMSAQQLMSQSYKLHAEYRTDWYGAGYDM
                     LQDIPGYPSVILQAELSANQYVIESDNDANKWRQFGTTTYNQASLFLWYNSPVSDNEL
                     ADSTTQNKWYTTRIKDAGYTSTEGIVMETTNEPTQFAAVNTIVQTTPNPVYTNQNINF
                     TVNMAGPRSPEEFCFMRYSYDNFVTSDIVIVFFSGLNDLTGNATISATATEKTVQVYA
                     FTSTYDAWNFPASDFDLITLRRGLNNGNTVSVTTFDPPSASTVRFRVDMKNVTPGPNG
                     PHVVGSFNNWDSTATPMTQGVGSLWFADVVVDTNDILQYRFLGDDTYAAQETVPNACG
                     VDDGFGVYNRKLNVPNVANTTLPVVCFSSCAACVYPDTVLVTFRVDVGGPAPNGVSVF
                     GSWDNYAGTALGLIGGTTWGAAIKLDTTTQVLYNYKKGTLAENFYGNCTKPNGLGGNY
                     RFLNVPEANTVLNIVCFNNCQNCANNNKVTVTFRVDMTGTPLGPGNAHVVGNFNNFNP
                     FATLFYPKGNGIYEATALIDTTQVIYYKFLRDASFTGEEKVPEACGVFYGAWGDTLRK
                     LTIPENDTLLDVVCYSSCIACQPTSLVEITFRVDMNIVPVGPGGVHMAGNFNGWNTTQ
                     IPMTQEGSTTIYRCVLKLDTAIRIKFTYFSDTTLASHENIPSNCGHGGNGQNFQGREY
                     DVDQVSTVLLPVCFGYCSCNNVGKVKVRFRVDMANTTIGNSYAHIVGTFNNFDPTATI
                     IFPVNGTIYIQDVLLDTNTTYQYKFLRDANFIGAEVVPSACGVPDGNGGYLREVYIGY
                     QDTMIKAVCYGGCSGACPPNSQKEITFKVDMSNTTIGPGGPHVIGNFNNYDVTATPMI
                     DGGNGLYYAAVFVDTSQTIIYKFLKDSSLVDQEQVPVACDDANDFRQVYMGNTGQIVP
                     TVCYGSCTTCKQGQTADVTFRVDVGQSGFGSGVYLATSDNGFNPLQMNSLGGSVYSLT
                     LNLDTTLSLDYYFINNLGPGGDEIVPSACGVLNGFGNYYRHLEVPESPLLLPNVCFGE
                     CVLCPPPPLSDVTFQVNVSNTNIGANAMYLAGDFNSWNTTSLLMTNYGNGIWKRTVTL
                     DTTKTIQYRFFVGNTFAGSETVNSACGITDTVGGLSRQLEVPETDVILPAVCLNECAN
                     CPANVNVTFRCNFAQTTIGAGKPHLAGTFNGWSLTATPMDSIGNSIYEKTLTLDSTLT
                     YEYKFLSDTLTTAYEIVPNACGINNGGVYNRKLIAPEVTTVLPAVCYASCNPCVFPDS
                     VDITFKVDLSLVTLGNGGPHLVGSFNNWNANKNPLSQITGNIWGTTLRLDTTEVIEYK
                     FTTDNNNASQELVPVLCGTPNVFYGLVRKLIVPQVDSTLTTVCFSSCVACPLPVTANV
                     RFSVNMLQATIGAGKPHLTGTFNGWSNTLTPLDSIGNGIYEVTLQLDTTSTIQYKFLN
                     DTTFNTVETVPVLCGVPDGFGGFNRSLAIPESDILLPTVCYSACTDCIFPDSVDVTFQ
                     VDLAQVTLGPGGPHLVGNFNGFDVEATPLANVTGTIYAATLRLDTMLNAEYKFLVDTS
                     YINEEIVPQACGISNGFGGFNRLTFVPENDTVLPVVCYSSCAACVVPDSVDVTFQVDL
                     AQVTLGQGGPHLVGSFNGFDVKATPLANVTGTIYAATVRIDTTANVEYKFLVDTSYIN
                     EETVPQACGVPNGFGGYNRFLSVPENDTVLSVVCYSGCAGCVLPDSVLVTFQVDMAQV
                     SFGTGGPHIVGTFNNWDVTATPLTFIGGTIYGKTILLDTTDYVEYKFLVDSSYINEEN
                     VPQACGVPNGFGGYNRFLSVPENDTTIKEVCYSSCAPCVPFGLVNVTFRVDMKNAPLG
                     PGGPHLVGAFNNWNPTATPLNNLGNNIYGITLPLDTTKVYSYRFLSENSYNNTEVVPA
                     ICGIADSLGVFNRFIDVPEFSTILPIVCYSECGPCTPTANVLITFQVDLAQVTPVSPH
                     LVGSFNGWNATATPMWNFGGTVWGVQLPLDSTLIYEYKFLVDSSYANEETVPAACGVP
                     NGLGGFNRQVIVPEASTILPLVCYSSCIGCVNNNVNVTFKVDMHGLQILSYNPIGLGG
                     VHIVGSFNGWNTTATPMTDANNDSIFEVTIPLDSTTTVQYKFLRDTNYTQSDPVPALC
                     GVADGFGGYNRQLQIPEFDITLQEVCYGFCSGCFVGVQDLSSSNWVAVYPNPVDEILT
                     IELDMNNSGNTNILLFDAKGALVKNVNAASAINRLDVSALDPGIYLLQVISENGIVNK
                     KITVR"
     gene            9773..11608
                     /locus_tag="PK736_01195"
     CDS             9773..11608
                     /locus_tag="PK736_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRC32038.1"
                     /translation="MNLKNSIVGFAMVLLTTKLFSQTPIVADIGTVASEEASSIVQAS
                     DGGFALLFNGSINPPINNLTGCTFLRTDCNGNVLFYKTYAPGQANAGYHLTATSDNGF
                     VFLMAVNSNNINSTYITKIDSAGNVKWSKIIPVRSGNMDGLSLDIAGNIYVVANASLT
                     NVTKDEIALLKLSPSGNLLFSNKLEYTSNLRASDISISTSGQIYILAMANVVSTTYTD
                     IVLIACNAMGAVTNAKTYTTALDDEPAQMLISNKNELVICGRSFFSNSAYDGFALKCD
                     SNLSYVTSKYYDASTQLGEMWRTIFSDGNSGWIVAGDKGAAGGRDFICANLDSNFNMV
                     WSNYYPIAPQFTNYTYAGCRNINGSITLTGDLRSTANLRQTLFLTIANSGNAPCSTQV
                     LPMTTTSEVPAVLNVSIAQSQIVFTPIDNMPAMATINVYSNGICGVNNSCLNFTTSKQ
                     GDCPAQCYVITNTSSAAPGSFWYWDFDGGDPAFSLQQIPPVVCFKEKGVYNIKLTVTT
                     NGKSNSVEREINTEEDCAITIPNIFTPNGDNVNDVFFIKNLPDNFLLTVVNRWGNIVF
                     ETKDKNKFWDGTNQKGTFVSEGVYFYILQSDTFKENYRGTVSILY"
     gene            11627..12460
                     /locus_tag="PK736_01200"
     CDS             11627..12460
                     /locus_tag="PK736_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC32039.1"
                     /translation="MKLNKPDNFRSNKPSILFIHGFASNHHVFDTNIAVLTKNFNCFT
                     LDLFAGIHSSATMPNVHVEHFALQIVEELKKLQLASLTICGHSYGGQIAIHAAAHSPG
                     LIEQLILIAPSGIETFNQFEQMMAQPFIPWAARQISGNAEDFLRFAFRNYSKDAHAFV
                     SKISADYISQTGKHIQSVTTKVFAEIISAPTFSLLQKITKPALIIFGEVDQIIPNMMA
                     HPFLSTRIIAANAAKQLPCGSFKLIPHSGHFVMWEQGALVNELIYKFAYTSTVIQPSL
                     Q"
     gene            complement(12438..13520)
                     /locus_tag="PK736_01205"
     CDS             complement(12438..13520)
                     /locus_tag="PK736_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32040.1"
                     /translation="MLKYFFIVFMLVAINNSIGQENPGWLPLELKYNKEVHLKNVKQL
                     TFGGDNAEAYFSFNDSLLTFQVTNKQWNLECDQIYWFDWRKDDLQKNIPHLLSTGKGR
                     TTCSYFLPGDTTILFASTHLGKETCPAPPAKRTDGKYVWAVFPDFDVFVSDLKGNIIK
                     RLTDSPGYDAEATVSPRGDKIVFTSVRSGDLELWTMNTDGSNLKQVTDKLGYDGGAFF
                     SPDGSKLIFRASRPVTEEDQKTYIDLAKEGLVMPTLMSLYICNVDGSGMQKITDLANA
                     SWAPFFHPSGKKVIFASNYNSKRGLPFNLYMINIDGTGLEQITFDTSFDAFPMFSHDG
                     KKIVFSSNRNNNGTSETNLFIADWVE"
     gene            13723..14379
                     /locus_tag="PK736_01210"
     CDS             13723..14379
                     /locus_tag="PK736_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRC32041.1"
                     /translation="MMMKKVGIIGSGNVGKALSHGFLLNDYEVMIGTRDTHALLEWQV
                     AEGANAMPGSVKEAAEFGDILVLAVKGDAAMAVMEQLDVASYAGKTIIDTTNPIDNSK
                     PPVNGVLSYFVSGSDSLMEQLQRKFPQANFVKAFNSVGSSFMVNPKFPEGKPTMFICG
                     NNADAKKKVEEILTAFGWGTQDSGMVESARSIEALCQLWCAPGFLRNEWTHAFKLLKM
                     "
     gene            14595..15056
                     /locus_tag="PK736_01215"
     CDS             14595..15056
                     /locus_tag="PK736_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC32042.1"
                     /translation="MKHFDKILCPYDFSPHSEEALRYAMKLADNTTGITLLNAIQLPY
                     LIDPNGFSYYDFKADELKKSTEDSLAKKVEEVKSTYPDLNFDFKIEVDNDPAEIVLKV
                     QKEGKYDLIVIGSHGRKGLGRILMGSVAEAILRDADCPVLIIKMIKPKTAA"
     gene            15125..15502
                     /locus_tag="PK736_01220"
     CDS             15125..15502
                     /locus_tag="PK736_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32043.1"
                     /translation="MKDSISHLHVVCEDWMRELQFYKNEIPFFKKRIEELSTKYTTKE
                     VLAQIEHFENKFHIMDTHCDELIHDVKAKNKWLLNQASEQPKFIGVKMIAADENIPDL
                     MEFTARDFASTKKEFYEFLSKYM"
     gene            complement(16148..17602)
                     /locus_tag="PK736_01225"
     CDS             complement(16148..17602)
                     /locus_tag="PK736_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRC32044.1"
                     /translation="MQETFQLRLSQTGFILGPIVFVFIWLFFPHNSIHPDAYKVLAIA
                     SLMIIWWLTEALPLPVTALLPLVLFPATGVMSMSDASLSFGNSVIFLFLGGFMIAIAM
                     ERHGLHERLAYKIIHITGTSSNGIILGFLIATAFISMWISNTATALMMLPIAHSVIEI
                     SKTKEKPQLNKDDKNFALSLMLVIGYAASIGGMATIIGTPPNVVFVGLYEKFYHQKIN
                     FLQWMKIAFPATIFISALFYFLITKVMYRVKNQARQSSSNFIHEKLLLLGPLQKTEKI
                     VLAIFGLTSFLWITQQPLNNLFGSNILNDTNIAIGGAILYFIIPVNFKKFEFVLHWQD
                     TAKLPWGILLLFGGGLCLASGLEKTGIVQFIGNYVLSQNLSLDVLMIALIIITVMLSE
                     IMSNVALVQVLIPVVFGIAGNDSHKALMMGIPVTLAASVGFMFPMATPPNAIVFGSGY
                     IPIRKMVSVGFWLDVICIAIVYLVTKLTLPFIIS"
     gene            complement(17604..19643)
                     /locus_tag="PK736_01230"
     CDS             complement(17604..19643)
                     /locus_tag="PK736_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRC32045.1"
                     /translation="MRKLTLLCILIFSDHSLHSQQGMWTWMNGSTNANPSGVYGIQGV
                     PSVLNSPAGGYEGAMGTDKAGNFWFFSGFNTGVYDDLWKFTPATNEWTWMKGSGNTFT
                     GPVYGTQGIPAISNSPGNQQWCAAAWVDTAGNFWIFGGSDGGGTSYNLLWKYDPAINM
                     WSWEEGSTTCCTPGNYGTKGIASPTNQPPSRWETNAAWVDSSNQLWLFGGFTTTGDFN
                     DLWKYQISTGEWTWVSGSSVTNSNGNYGIKGVPAATNEPPPRKAHSHWKDSDGNFWMF
                     AGARTLGSFNDMWMYNSTTGFWTWMGGTDVLNDVGTNGPNCNADTSYLPKFRFENKAS
                     WIDKQGRFWNYGGSNLSDLWVYNPKCNSWALINGNPSVFTAPVFGTQGIAAPGNEPGG
                     LMGAVSWMDSSDGLWMMGGWNNNYNAVNTLWKFVIDTTCAISFCGNIPQCNLSSSDTL
                     FCEKQCIDFYDLSVNSPTTWQWQFTGGSPSTSTMQNPAGICYNNYGSFDVQLIACNNA
                     GCDTVFFPAFITEFQSPAAPTILWQNDSLICTATNVSYAWYSTLNPNLVLGTNYFYVP
                     FTAGTYYVLITDSNGCEVPSLSIAANVGWNDITQNSIQIVYEGIESNLFLNCPENLLD
                     EYTVTIYDGIGRVVMEIKSNCNSKKITLPETLSGGIFSVQISSSKMNMTRRFLKK"
     gene            complement(19653..21731)
                     /locus_tag="PK736_01235"
     CDS             complement(19653..21731)
                     /locus_tag="PK736_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC32046.1"
                     /translation="MQKNKITFYIFLSLLICVTKSYSQTKFYETKFNGGVTCAGYSPG
                     VSPNANGVFQIHIAPGSTIHKAFLMCGEMGDGSTANFLLDGNQVSFIHNVTQVSPKFG
                     NQLYGDSAAVHAIDVTSLIIPTDTVYTFTGIQLNTTVDFFTDFYLYVAYANPALPSIS
                     SAIFLNTTAMGQSVSHYLKLQNKFPASGDVALAFLMGYMCGSGDGENVAVNGTPIGLA
                     YGPDANNLPGSCGGPAASFYYENKILTGLNDDIANQAITGPEVLSNINGLVTPGSDSI
                     FVQYNHQNNGVDNSIWAAIVTYNDAIEIKNFCFGDTTLFLAQDSIGYVAFQWNFDDPS
                     SGPNNISYYANTSHVYTAPGNYVVTLIRALTVPPFVDTSYYPITINPTPTISLGSDTT
                     LCIGQTLVLNPGIYNSYLWSNGTTLNSLSVTANSTIAITVDNFGCKASDSIDVTFTPC
                     ANTVANLSCTDTIFCEKQCIDFFDLSTNSPTTWLWQFTGASPSSSTLQNPPGICYNNY
                     GSFDVQLIACNNIGCDTLLLPSFISEFQSPPAPLVIWQNDSLICTSTNVTYAWYSTLN
                     PNAILGTNYFYLPNIAGTYYVVIADTNGCDVSSIGVIANVGWNDLTQSNIQISFDNTE
                     NSLLINCNGFNTDDYTITVYDGIGRNVFTEYFNCSELSIKLPSSFLPGFYLITISNNK
                     TKIEKRIVKL"
     gene            complement(22325..23599)
                     /gene="nhaD"
                     /locus_tag="PK736_01240"
     CDS             complement(22325..23599)
                     /gene="nhaD"
                     /locus_tag="PK736_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HRC32047.1"
                     /translation="MVILAVVLFIVGYLAIAFEHNLHVNKAAPALLIGTICWLIYFLG
                     ATDMHVATENLNHSMSDIAGILFFLLGAMTIVELIDSHHGFDVITQKIRTRSKSKLLL
                     IIGVLTFFLSAVLDNLTTAIVMSSLCTKLLKDKEDRLWFAGLIIIAANAGGAWSPIGD
                     VTTTMLWIGNQITTGNIIRYTFLPSLVVLIVPMLLMMSKFKGQTIPMVNHEINNDYQS
                     EKKWVLYAGIGMLIFVPIFKTVTHMPPFMGMMMALGFMWVLTSQLHKRKEETLRNKFS
                     LHQALQRIDTPSILFFLGILLAVSSLEQLGILHSLASYLDTALKNEYIIGTVLGMLSA
                     VIDNVPLVAAAQGMYDLTTYHTDHYFWQFLALTAGTGGSCIIIGSAAGVAIMGLQNIN
                     FIWYLKKISWLATAGFFAGIATYILQSKLLGL"
     gene            complement(23708..24718)
                     /locus_tag="PK736_01245"
     CDS             complement(23708..24718)
                     /locus_tag="PK736_01245"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRC32048.1"
                     /translation="MTSTINTAFSRQLQLRAPIIMAPMFLVSNVAMIVEAMKGGVVGT
                     FPTLNFRDDAALTNALQTISKYKTDTNCAGTYGVNLIVQKTNIYFEQHLKICAAQKVP
                     VYITSLGSPSQVVDTVKPYGAKVYSDVTNMTHARKAASTGIDGFIAVGQGAGGHAGPY
                     PLSLLVPALKREFPHLQVIAAGGIGDGATMLSALACGADAVSIGTAFIASEEATVSQE
                     YKNAIVDSGMENIVMTERVSGTPCNIIDTPYAKKIGYKQNWLERWLNTNATTKKYFKT
                     LVQYKGMKNLEKAIKPGNYNNLWSAGQSAELVHSIKPCAQIIDDIINETIAQMEILKQ
                     NF"
     gene            24826..26604
                     /locus_tag="PK736_01250"
     CDS             24826..26604
                     /locus_tag="PK736_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRC32049.1"
                     /translation="MTEVTRLFDVVPYQLEKFPKADSLAGKDNGAWVTYSTQQFANRA
                     KEIAMGLIALGINRGDKVVSISNNRPEWNMLDIGILQTGAVHVSIYPTISESEYKFIL
                     NDCEAKLIVVSDEALWKKIDSIKAEVPTLQNLYTFEKMLGKIHWLEIINAGKSSDESV
                     WQMRMDAVMPTDLATLIYTSGTTGNPKGVMLSHSNLVSNFIACRDLPPVDSRHRALSF
                     LPLCHVYERMLTYLYMYIGVSIYYAESMEKIGDNIREIKPHVFSAVPRLIEKVYDRII
                     NKGKELTGVKRALFFWAVELGLKYEHGNRNGIVYQTKLKIADKLIFSKWREALGGNTM
                     CIVSGGAALQERLARAFWAAGMPILEGYGLSETSPVIAVNLLGDNLACFGTVGTIIKG
                     VDVKIADDGEILFKGPNLMMGYYKRDDLTKEVIDNEGWLHTGDIGTWVEEKFLKITDR
                     KKEIFKTSGGKYIAPQPIENLMKESAFIENIMVIGESQKHASAIIVPNFAHVKSWCEI
                     HKVTYTTPEEIIKNPQVRDRIMKDVEKVNAQLGHHERIKRSELITKEWTVVSGELTPT
                     LKLKRKIIEQNNKDLIDKIYSVDDEK"
     gene            complement(26779..28074)
                     /locus_tag="PK736_01255"
     CDS             complement(26779..28074)
                     /locus_tag="PK736_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC32050.1"
                     /translation="MHFNSLGIIEPILKAIQEEGYTTPTPIQQQAIPIVLQGTDLLGC
                     AQTGTGKTAAFAIPILQLLSGVHTNDKKLKIRSLIVTPTRELAIQINESFKAYGRHTK
                     LRSTVVFGGVAQGPQTTSLQHGVDILIATPGRLLDLMNQGYISLRDVEIFVLDEADRM
                     LDMGFIHDVRKLIAVLPAKRQTLFFSATMPPEIVKLSSSILHHPKKVEVTPESTTAET
                     VQQYIYFVDRGNKNSLLIDVLKDTKIKTALVFTRTKHGADKVVKVLEKHHIKAEAIHG
                     NKAQNARQRALSNFKAQSTRVLVATDIAARGIDVDDLQYVINYEMPNVAETYVHRIGR
                     TGRAGAAGTAFSFCDAEEKAYLRDIERLISKRIPVIDDHAFPLIDNHPIPTEQKQHQR
                     RSQSGGRPQQGSTKPHAGAKQEPKPQKNWQRNKTRHTSA"
     gene            28603..29889
                     /locus_tag="PK736_01260"
     CDS             28603..29889
                     /locus_tag="PK736_01260"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HRC32051.1"
                     /translation="MLGYVEHREALIWVQTACNKSVTIKYKKQNDSKIFTKSISNINT
                     DKCEPWIAKFVLPLLDVNSEYTYQIFLDNAPVKFSYPLKFKTEKLWAPWSTNDPYEIN
                     FLFGSCNYVNDSAYDRPGKPYGQSTTIFNAMAKSNADFMIWLGDNTYLREADYSSISG
                     INYRYLHTRVEKNLQPFLASMPQYAMWDDHDFGDDDASKNFAFANITRQCFIDYYGNN
                     QYGENGQGTYFKFVKSDADFYLTDGRTFRDESKLDEKIFPQKTMLGKQQLEWLKNNLA
                     HSTSTFKFICLGGQFLNSETTKESFNLFENERKEIMQFIADQKISGVIFISGDRHHCE
                     LLKYDSLANPGYAVPYTLYDITSSPLTSGVSGILNSPEKNNPHRVANTLFVGNNYCKM
                     SITGKRNERVVTLFCYDRDGVEKWKYEIKEIELKMH"
     gene            30004..30543
                     /locus_tag="PK736_01265"
     CDS             30004..30543
                     /locus_tag="PK736_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32052.1"
                     /translation="MDQEDLGIGSRVSHPEFGDGVVINVKTRTYEIVFVRSGRMEISK
                     FQNALTVIEVVEPDTDLVSKSEMEVSLINILKRYSDLQEVIPLGQKWIGGKIIMQPGN
                     TSLKGKEISIDAFFHKIVMLRDRLRVMEQRINSHSIMTDEDKVNMQQYITRIYGSLTT
                     FNDLFKNKEDNFVGDKGGE"
     gene            30849..31184
                     /locus_tag="PK736_01270"
     CDS             30849..31184
                     /locus_tag="PK736_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32053.1"
                     /translation="MISKQKTNEEKEAIIAEYLLGEKTFRQMQSLHGVDYRIIHSWVT
                     KFKGHHVQKLKTPTETIQFYEQQECDVKALREELRKAKLYNELLNTMIDIAEDQLKID
                     IRKKSGTKR"
     gene            31220..32092
                     /locus_tag="PK736_01275"
                     /pseudo
     CDS             31220..32092
                     /locus_tag="PK736_01275"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(32423..33022)
                     /locus_tag="PK736_01280"
     CDS             complement(32423..33022)
                     /locus_tag="PK736_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRC32054.1"
                     /translation="MNRPLVFPKKDDNDKELLKLVEFFNETLGFCPNSVKTMHHRPHL
                     AYAFIELNKAVMENKGHVTSALKRLIGYISSNVAGCKYCQAHTIRAADRYGASHTQLE
                     NIWEYKTHESFNDAERAALDFAVAASTIPNAVDDAIAENLRAHWNDGEIVEILGVISL
                     FGFLNRWNDSMATELESGAIQSGEQFLSSRGWSVGKHGF"
     gene            33293..34303
                     /locus_tag="PK736_01285"
     CDS             33293..34303
                     /locus_tag="PK736_01285"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HRC32055.1"
                     /translation="MKSTSKEVNAFFLSTKKAIYVVVLGLSFSANAQSPKTVLVEHFT
                     NTWCSICASKNPAMYQTLSNYPQVLHIAFHPSSPYPGCVFNVQNMAENDARANYYNAY
                     GGTPKIVLNGKLLSSNTPLINNTTIDTALNKNSPVEISATELLIGLDSMKARVVVRTT
                     GITNITNVRLFAGVAENPVNYTSPNGEQIHHDVFRKALTAAVGNQIVLPQLNDSLVFE
                     FTYFINPIWNVNAIYTMAYVQIEASKEVLNAVKSIRVLANSLNENNENDFAIHFNSIT
                     NQLVVASYNFKSNTHYSILNIKGQVIAAGDASTSEITVNNLPKGVLYFRIGRKVMKFV
                     NL"
     gene            34776..36137
                     /locus_tag="PK736_01290"
     CDS             34776..36137
                     /locus_tag="PK736_01290"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HRC32056.1"
                     /translation="MQKLNLRLLAMWSLLIVSFTSCMRENEEPVISNSGNDVNQTSSE
                     KKMNSNEIPTAWMDLTLKLIKTTPGYSPPVASRTLGYLGVTLYEATVSGSVVNKSIAT
                     QLNINPVPPAPQPNKYYYWNICANASMAQAARKFFITTTAANKVSIDSLENYFNAQAL
                     ANYNNVVVNNSITYGRNVADAIYNWSATDVVGHEGYLKNVDPNYVPPTGPGKWIPTPP
                     LFAPAVQPHWGDVRPFVAQNPNIWMPNPLTYSLSPQSNYYKQALEVYNTKINLTAAQS
                     TIAQYWADGGGTITPPGHSMNIATQVIRKEGNNLYKTAEVYSRVGMALADGFIQCWKE
                     KYVHNAMRPITYINYYIDPAWTPLIATPPFPEYSSGHSSQSGAASEVLTAIYGANYSF
                     TDYSKVPDGFAPRSFNSFYAFADEAAVSRLYGGIHFRAANENGVICGRKVGSNIMKIV
                     YKK"
     gene            complement(36189..37376)
                     /locus_tag="PK736_01295"
     CDS             complement(36189..37376)
                     /locus_tag="PK736_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32057.1"
                     /translation="MSRYTSQLQRIQSIIESYTGETPFQHYLKNYFTKNKNAGSRDRK
                     IIRNACYTFFRTGNMFKHFLFTEKLACSYYLCSDATDEFIQSVFDASLVQPLQNSINK
                     PLNEKLQLLKKQYPEIDLKDLFPCYNNLSENFKNESFLFSHFNQPNVWLRVNNGFIDF
                     VKQDLTNNKIPFEFETNIIKLQPASNATDLDSFAKGGFEIMDKSSFATTQLFEVKDNE
                     TWWDACCGAGGKTLALIDTLQTKEYKNVSIACTDIRIPILKNLKERILKTGFTKFKNQ
                     PMNAAEDQWPDGVLFNKIIVDAPCSGSGTWGRNPENLLQFDSLLLPVFSQKQFDILNN
                     CLQCLKKNGVLYYITCSVFADENEGVINKIDKEKYSVLFSKYISGSDDGADTLYISAI
                     KKA"
     gene            complement(37787..39067)
                     /locus_tag="PK736_01300"
     CDS             complement(37787..39067)
                     /locus_tag="PK736_01300"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRC32058.1"
                     /translation="MASIDRTFQPALNAVDNFKLTKANKIMLDNGMPMYVIDAGTNEV
                     CKIELLINAGMDKPTNPILAQVACELADDGTSTKTAYDIAETVEFYGASLETNIEFHN
                     ASFCLTTLTRHLPNLLPLMSEIIYDATYPQTELDSYFQRSLQSLKVQQQKVEYLARKT
                     FSKKMFSGFPYQHSNDEEDFLSLTQKGLTDFHTQNYVGTNYSLIACGKINDDVVKQLN
                     SIFGVQKINAAKNEDKKYTYDNALENVFIEKENAVQSAIRIGKRAITRHHPDFPALSI
                     LVTVLGGYFGSRLMSNIREDKGYTYGIGAGLVAYPKAGSITISTEVGSDVCDAAIKEI
                     YFEINRLCTEPIPEEELNTVRNYLSGVFLRSWDGPFALAERFKVLLTNNLVYSYYENY
                     LNVLVNITSNDLLGIAQKYLNDADWLQVVCGSRK"
     gene            complement(39070..40317)
                     /locus_tag="PK736_01305"
     CDS             complement(39070..40317)
                     /locus_tag="PK736_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRC32059.1"
                     /translation="MISYERYVLPNGLTVILHHDASTPIVAMNILYKVGARNEVETKT
                     GFAHLFEHLMFGGSVNIPVYDEPLQRAGGENNAFTSNDFTNYYLTLPKQNFETACWLE
                     SDRMMQLAFSEKSLDVQRHVVIEEFKQRYLNQPYGDAWLYLRPLCYTTHPYKWATIGK
                     EISHIEDATLEDVKSFFAKYYHPANAILSVAGDIDFGYTKTLIENWFGNIKQQALEIS
                     PLPQEPKQTELRRQILERDVPASAIYFAFHCCSRYDKEFYATDLLSDILSRGKSSRLY
                     FQLVKQKKIFATISAYMLGELDKSLFIIEGRLNDGKTFAEAEKAVDEVLQEIMDGKID
                     DRELQKVKQQVEASLVFNELGVANKALNLCYYDMLGDVAAVNEQAEKYNDITKQDLAN
                     IAQQIFAKDNTNILCYQPITKTN"
     gene            40591..42885
                     /locus_tag="PK736_01310"
     CDS             40591..42885
                     /locus_tag="PK736_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRC32060.1"
                     /translation="MIKKFLSIVSMLFLLANIANAGTTKWININSAKPAEATSKLILD
                     NGSQTVVEFNLPGFDMKAVSANNLDAASIIPKAAGTTPLLIDGAPDLSKYAFSVIIPD
                     NAKMKVDIENSSYIDYKDIKIAPSKGNLYRNTDPSTIPYVYGDSYKTNAFFPGKLAEL
                     QSPYILRDFRAQTVWLYPFQYNPVTKVLRVYYKLKVQIVNDGISNINVLQRKGTAKKI
                     DKEMCAIYNSMFKNGGNNTINYAQVDEDGSILILAYNQFMSTLQPFVDWKIREGIHCE
                     MIDAASAGATAVAIKTYIANYYNMHPELKYILIAGDAQQVPTPILSGGASDPSYGYLS
                     GSDSYAEVFVGRFSAQNITDLQVQVQKVIDYEINPNLSLQHFNKGVVVASDQGPGDDG
                     EMDWEHERIIRDKELTYTYMDIKELYDGTHPGGNDDPGDPSSADLFNKFQSGINVMTY
                     TGHGSNNSCSTTGLSSNDVDNMTNYEMLPFIWSVACVNGNFNQGDCFAEAFLRAQSNG
                     QPTGAIATFMSSINQSWNPPMDGQDEMVDLLVESYAANIKRTFGGLSINGCFHMNDAY
                     GAAGDEMTDTWHVFGDPSLMVRTAPPTVISATHDAYISLNQSTFTLQCNKQNGALACL
                     SNNGEILDVQPIVNGTATFNFNALIVLDTIQLTITGFNCKPYLTDLPVLLTGLDEQEN
                     NLSLSVFPNPANDMLTVTIKDFVSRTNNVEILSETGAQVMNLTFAENSSSIEVPTKKL
                     SNGVYFIRYQNGNDICKKQFVVKH"
     gene            42983..44314
                     /locus_tag="PK736_01315"
     CDS             42983..44314
                     /locus_tag="PK736_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRC32061.1"
                     /translation="MQVQATYKNIWSLAFPIITGALAQTVLGVTDTAFLGRVGETELA
                     ASAIGGVFYFTLIMIGMAVSIGAQIIIARKAGENDLNGISHVFKQSFLVLGGVGLLMF
                     FIAHFLAPVLFPYIIHDTKVTEAATSYVICCSYGLLLWIPLLSVRAFLVGISNTKLIS
                     IQAIIMCAINIVLGYIFIFGHYGFPAMGISGAAYASAISEVLASLYALSIIRFHKNYK
                     SYLLFDNIQANKATINTILRLSAPIVLQNFLTMGAWFVFFVMIEKLSTHALAISNVIR
                     ATYMIMMTPAWGFSSAANSMTSNLIGQGKADEIFLLLRKIITMSTTIITAFIFVMALF
                     PHYILNVITNDQQLIEDCIPTYYIICVATVVLSIGLNFLSAVSGTGATRTAMYIEMTN
                     IFCYLVFVYATCFIFKTRVEIVWLSEILYWTMMGVLSLLYLKTNKWKNIKI"
     gene            44293..44664
                     /locus_tag="PK736_01320"
     CDS             44293..44664
                     /locus_tag="PK736_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32062.1"
                     /translation="MEKYKNLIFVFAFAFAGGWQCGDTKEATKSSVPAETKIAPNIET
                     VAAGYMNAKVVDLTGLDGCKFVLELEGGKQLVPTNLKPEFCRADIEVSVKYFVPKGAV
                     NTCMAGTIIEITDIKIRERRK"
     gene            44661..45035
                     /locus_tag="PK736_01325"
     CDS             44661..45035
                     /locus_tag="PK736_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32063.1"
                     /translation="MKIQRPEQYFEQFSGEQKIILYVLHDFILQHEGVFCRIAYNIPM
                     YYRNGWMCYLNFKKNGDIEFAFTNGVHLPNKNGLLQTNDRKQVAGTFINDAMKINLDA
                     LEETMQEAIAFDNIKYKPRKRT"
     gene            45039..45983
                     /gene="fmt"
                     /locus_tag="PK736_01330"
     CDS             45039..45983
                     /gene="fmt"
                     /locus_tag="PK736_01330"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRC32064.1"
                     /translation="MRIIFYGTPDFAVPSLQILIENKFNVVAVVTAADSPAGRGLQMK
                     FSPVKEYAISQNIKVLQPHKLKDPVFLEEIKSLMPDLQIVVAFRMMPQVLWSMPPLGS
                     FNLHGSLLPQYRGAAPINWAIINGEKETGVTTFFLQQEIDTGNIIFQEKIPIDERDNA
                     TTLHNKMMHTGAALVLKTAWAINNGEVDATPQSKVDTNELKAAPKIFKETCAINFNNE
                     VDKVNNFVRGLSRYPAAYTYIVSKDNERLQLKIYSAQKEYGEMNVAAGNMQTDGKTFL
                     KFACNNGWLHALELQLQGKKAMTVQEFLRGFKFQNTNA"
     gene            45976..46665
                     /locus_tag="PK736_01335"
     CDS             45976..46665
                     /locus_tag="PK736_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRC32065.1"
                     /translation="MRKHIVVFTGAGISAESGIKTFRDSGGLWEEHRIEDVATPEAWQ
                     RNQALVLDFYNQRRKQLLDCQPNAAHYAIAKLETTYKVTVITQNVDDLHERAGSTKII
                     HLHGELRKARSTANPRLLYDIDGWELKQGDTCELGSQLRPHIVWFGELVPMIDTAAYI
                     AEQADIFIIIGSSLEVYPAASLINHAPHSSIKYLVDPNAKPLPHIENLTIKKGKAAHE
                     VPALVEKLLIA"
     gene            46665..47264
                     /locus_tag="PK736_01340"
     CDS             46665..47264
                     /locus_tag="PK736_01340"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HRC32066.1"
                     /translation="MEKLFTSTDPLYQWFLMPLFIFVCRASDVSLGTLRNILLSKNLK
                     TIVPFIGFVEVMIWLVAVQSVLSHVSNIAGYLAWGFGYAAGIYLGIRIDKRLGLGMQI
                     FHIITHHNITNLLEAAAQHGFAPTTVDATGSRGPVKIVYLIIDRTENANVKALIQTHC
                     PEAFYTIEDVKDANQGVFPNQQENKLLFYLKRLVPLFKK"
     gene            47782..48414
                     /locus_tag="PK736_01345"
     CDS             47782..48414
                     /locus_tag="PK736_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRC32067.1"
                     /translation="MSEKKTGNNKTHTTGRLTHVEKMHPFKTFLFFAMVGSTLIFLSL
                     VFLYAIRVSSSGVSQHFQLPRTFTLSTIILLFSSFALSRCLRAFKNDELRDLQISMLS
                     TFILAIAFCITQFIGWKMLADAGYLVISNPGVTFLFIISGFHMLHIVAGLGAFGYLNF
                     QIFHATGNVAKALLFLSDNSNRTKLQLFTIYWHFVDFVWLCVFVMFLFTL"
     gene            complement(48863..51064)
                     /locus_tag="PK736_01350"
     CDS             complement(48863..51064)
                     /locus_tag="PK736_01350"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRC32068.1"
                     /translation="MLKKIVLASAVALFMNVAAQSQHSHRNCGTMDALKEQLQNDPTL
                     KQRMDAQERDIATYVQQNQNAKSNVITTIPVVFHILYNTAAQNISDARIFAQLDVLNK
                     DFARLNADAGNTPGVFSGIASATNIQFCLAQRDPNGAATTGIVRKATTVTSFSSAGNP
                     MKFSASGGSDAWPTGTYLNFWVCNLGGGLLGYAQFPGGTANTDGVVCVTGSVGGPSAP
                     GTSTPYNLGRTATHEVGHWLNLSHIWGDANCGNDQVADTPTQQTSNFGCPTFPKVTCG
                     NGPNGDMFMNYMDYVDDACMNAFTAGQSLRIDATINGARASLKTSLGCTPPNGGCGTP
                     ASLSASAITSTSVTLNWAAVPGASSYNVQYRVVGAGVWINTTSTTNSKGLTGLVASTN
                     YEYRVQAVCNGATSAYSSIGNYTTSSSGGCGVPNSLSATSITSTTATLNWSATLGALS
                     YKIQYRVVGATVWVNKTSTLNTKPIAGLVASTNYQYRVRTVCAGGVLSAFSTISTFTT
                     SAPGGNCANSYEPNNTNATFTVINVSTDIYSMIGTSTDVDWYKFSTVSPNIKIKATLS
                     QLPFDYDLELYSYGNVLLASSLLAGTSDENVIRNATTTGSFKLRVFGYNGAFSVSSCY
                     KLRVNVSSTNWTAMMPLNERNPKVAEELDINAYPNPTTSDIAFVFRSDVEGKGTIVIT
                     DITGRQVHSVNINVIEGTNTATMNIESLTEGVYVAQIYTGSEVLKVKIVKE"
     gene            51355..51912
                     /locus_tag="PK736_01355"
     CDS             51355..51912
                     /locus_tag="PK736_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRC32069.1"
                     /translation="MLLLLFFAMVGSTLIFLSLVFLYAIRVSSSGVSQHFQLPRTFTL
                     STIILLFSSFALSRCLRAFKNDELRDLQISMLSTFILAIAFCITQFIGWKMLADAGYL
                     VISNPGVTFLFIISGFHMLHIVAGLGAFGYLNFQIFHATGNVAKALLFLSDNSNRTKL
                     QLFTICWHFVDFVWLCVFVMLLFTL"
     gene            complement(51941..52597)
                     /locus_tag="PK736_01360"
     CDS             complement(51941..52597)
                     /locus_tag="PK736_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRC32070.1"
                     /translation="MNSLIEENYLKALFTLSNSKGEVNVNELSKRLEIKMPTVTSMMK
                     KLSEKKLVLYESYKPLRLTEKGKKEAAIVIRKHRLTEMFLVQKMGFGWEDVHPIAEQV
                     EHIQSPLFFDKMDELLGFPKLDPHGSPIPDKNGKVVWKEFNKLSECKSGDTIKLTAVI
                     NTSGEFLKFLNSRELSLGLKLKIKSVEPFDKTMIVSYGKRPSETLSHTVCERLLVEKI
                     "
BASE COUNT        16203 a         9887 c        10243 g        16353 t
ORIGIN      
        1 gataataaat atatattgat aaacaataaa taagtatcaa taattaatat atatttcatg
       61 tttctcattt ttgcacatat cttcctgtca ccttttaaca tgttaattgt ttcagtaaaa
      121 agtgtttcca aattatgagc ttttctaaga tttacggctc aatttcgatt ctaaatgtta
      181 tcagcgaagc catgaccata aacaagaatc gaaatttata atcccagaaa accatgaacc
      241 aattgaacaa aaaccaaata agcaataact aactacattt gccccgcgaa aaaaaataat
      301 gacagaacag tttcaacttt ggcttaataa attcgatttc ccaaaaataa tattggcgaa
      361 aaaaatatgg cgtaatatta ttgagaaaag acaacagcct gcaatgatat ctgcattact
      421 gaaaagtgat ttgcgaagca cgctttccga aagtatcatt actacttctt ttataagtct
      481 ggccgattta gattttagca atctcgatat ttctactgca aattatttat gtcaaaagta
      541 ttgcactcat catttcgatt tgctcggaag tggatgggta agtttaaatt ataatcaaaa
      601 agcattgggc gttgagggaa atgtgtatga aagcagcttt tcggatattg atttcgattt
      661 cgaaaataat tggattgcca aggttttgcc ttcgtcttac gttcaggaaa gtaaaaagct
      721 gtttgatata attcggaaga ccaacaaagc atataatccc atcgactggc accgagattt
      781 taagtcgggc tttcgcttcg attcgcaaca aacacacgct actcaatatg gacaaattaa
      841 aggcgaaggc atcgatatta aagtgccctg ggaattggga cgcttacagc acttacctca
      901 gttggctttg tttgctgcca ttcttcccga tttaaaaaat acaatcatta cagaatatgt
      961 ttgcctgatg tatgatttta tggcgctcaa tcctgccggc attggcgtaa actggagttg
     1021 tacaatggat gttggtattc gtgcggcaaa tatgttggtg gcttatgata ttttttgcca
     1081 actcgattcc ggtaatttaa tcgatgagac ttttaaacaa cattttgcca attacgtata
     1141 tagtcatggt tattttatta caaaccattt ggagtatcag gaaggaatgg gcagtaatca
     1201 ttacctgagc aatgttgccg gtttgttgtt tgtggccagc tatttacaat ctaatgaagt
     1261 aaatgataac tggtggtggc tatcggttca ggagttgtta tttgaaaccg aaaaacaatt
     1321 ttttagcgaa ggatccaatt tcgaaggttc cacaagttat caccggctta gtggcgagtt
     1381 aattacctat tgctcggcat tggtatgtgg tttgggaacg ctccgttggc atcaattaca
     1441 aaagcacaca caaaagcagc ttcaaaaaga gccgtttata aattttaaat ttttaaacaa
     1501 cgttaaaaag gatttagtgg ctgtgtataa gtcgggtagg gtagaaggaa ctattggcgg
     1561 taatttgcag atggtatata aagcggcatt gttttcaaag aatattacca aacacaatgg
     1621 agaaataacc cagataggtg ataacgatag cgggaggttt tttaaatttt caccttgcgg
     1681 aaaaatgatt agcaaccata atgcacaaga gaaatacgaa aacttaaaag gatatttgac
     1741 catgtatggt aatggtgatt tttgggatga aaataattta aatcatcaaa catatttaag
     1801 tgcaacaaat ggaatttttg agcaaagaat actcgaccaa tcgcaaaatc ttttcccttt
     1861 cgaaaattca ttagttaaat ctattatttc gaaaaacaac cagggacgca agttgatttt
     1921 ttatgctgac tttttcgggc aggatcaaac cttactaaat aaaaatcatc ctgcaaattt
     1981 aaaacttgac gaaaagaatt tagtccataa acaatctaag gtgtttgggc catatgcatg
     2041 tggcgatacg ctattcaata acctcaaaat aattaccttt aacgagttcg ggctggtaat
     2101 agtaaaaacg gatcatcttt ttttgagcat tgcctgtggc acaaataaaa attacattca
     2161 cagttggggg catacacata gcgacaaact tagcatagaa cttcaggtgg atggtgtaga
     2221 tattttgctc gaccccggta cttatattta cacaccctta ccaaatgcaa ggaatgcatt
     2281 tcgggcggtt gcaaatcaca acactttggt ggtgaataat gaagagcaaa actggtggta
     2341 cgatggcaaa aaaggtttgt ttaaaatgat gaagcaaacc aaatgcgaaa tgttaaacat
     2401 tggctctaat gaaataaaaa tttctttgca ataccgtcaa atcattcaaa ttcgacaatt
     2461 actaatttcg ggcaataaat tgatagtgaa tgatgccagt aataaaccat ttgttagtaa
     2521 ctggaataaa cttttttatt caaatggata tggaaaaaag ataaatcccg gtaatatgct
     2581 tgcctccaac gattagaact gtgtgttttc gggcttttca gaacatcacc attacttacc
     2641 ttaccgttaa gaattaattt atttcctatc ttcttcactt tatttttcac ccattttttg
     2701 aaacattcgt gtataactat tttgtattgg ctattgaata aacttcgtgg tttttatctt
     2761 cgcacaaaaa ttaaattgct taacaaatac gttcattcta acgtatacta tttaaggcaa
     2821 tacatagaaa acaaaaacaa gaaaaattat aaatctcaat atgaaaaaaa tctttccagt
     2881 attcacaatt ttagttgtga tttttacaat gagtgctcag caactaatga gtcaatctta
     2941 taaactgcat gcagaatacc gaactgattg gtatggtgcc ggttacgaca tgttgcagga
     3001 tattcccgga tatccctcag tgattttgca agccgaattg tcggcaaatc agtatgtaat
     3061 tgaatcggac aacgatgcta acaaatggcg tcagtttggt actactactt acaatcaagc
     3121 ttcactattc ctttggtaca attctcccgt ttccgataac gagcttgccg attcaactac
     3181 acaaaataaa tggtatacta cacgtataaa agatgccggt tataccagca ccgaaggcat
     3241 tgtgatggaa acaaccaatg agcctactca gtttgctgca gtaaatacca ttgtgcaaac
     3301 cacacccaat ccggtgtata caaatcaaaa tataaacttc acagtaaata tggccgggcc
     3361 acgctcaccc gaagagtttt gttttatgcg ttactcctac gacaattttg ttacctccga
     3421 tattgtaatc gtattctttt cgggtcttaa cgacttgaca ggaaatgcaa caatatctgc
     3481 aacagcaaca gaaaaaacag tacaagttta tgcttttact tctacttatg atgcctggaa
     3541 ttttcctgca tccgatttcg atttgataac cttacgaaga ggactcaata atggaaatac
     3601 agtttctgta accacattcg atcctccttc agcaagcaca gttcgttttc gtgttgatat
     3661 gaaaaacgtt actcccggcc caaatggacc acatgtggta gggtcgttta ataattggga
     3721 ttccactgcc acgcctatga ctcaaggtgt tggttcgttg tggtttgccg atgttgttgt
     3781 tgatacaaat gatatacttc aatatcgttt tttaggggac gatacttatg cagcacaaga
     3841 aactgttccc aatgcatgtg gtgttgacga tggctttggg gtttataatc gcaaacttaa
     3901 tgtgcccaat gttgcaaata ctaccttgcc tgttgtgtgc ttttcatcct gtgcagcatg
     3961 tgtgtatccc gatactgtat tggtaacttt tagggttgat gttggggggc cggcaccaaa
     4021 tggtgtttct gtttttggct cgtgggataa ttatgccggc acagcccttg gtttaattgg
     4081 aggaacaaca tggggtgcag caattaaact ggatacaacc acccaggttt tatataacta
     4141 caaaaaagga acattggccg aaaattttta tggcaattgt acaaagccta atgggctggg
     4201 tggcaactac cgttttttaa atgtgccaga ggcaaacact gtattgaata ttgtgtgttt
     4261 taataattgt caaaattgtg caaataataa taaagttaca gttacgtttc gggttgatat
     4321 gacgggcact ccacttggac ccggcaatgc acatgttgta ggaaacttca ataatttcaa
     4381 tccatttgca acattatttt atcccaaagg aaatggtatt tatgaagcta ctgcattaat
     4441 cgatactaca caggttatat attataagtt tttgcgcgat gcgagtttta ccggtgaaga
     4501 aaaagtaccg gaagcatgtg gagtgtttta tggtgcctgg ggcgatacgc ttcgaaaact
     4561 tactataccc gaaaacgata cgttactcga tgtagtttgc tatagcagtt gtatagcatg
     4621 ccagccaact tctttggtgg aaataacttt ccgggttgat atgaacattg tacctgttgg
     4681 ccccggtggt gtgcacatgg ccggtaattt caatggttgg aataccactc aaattcctat
     4741 gacgcaagaa ggaagcacaa ccatttatcg ttgtgtgttg aaactcgaca cagctataag
     4801 aattaagttt acatacttta gcgatactac acttgcttca cacgaaaata taccatcaaa
     4861 ttgtggccat gggggcaacg ggcaaaattt tcagggccgt gaatatgatg tagatcaagt
     4921 gtctacagta ttgttgcctg tttgctttgg atattgttca tgcaacaacg taggtaaggt
     4981 aaaagtgcgt ttcagggtcg atatggcaaa tactaccata ggaaacagct atgctcacat
     5041 agttggaact ttcaataatt ttgaccctac agctacaata attttccctg taaacggcac
     5101 tatttatatt caggatgttt tgttggatac aaataccaca tatcaataca aatttttgcg
     5161 tgatgccaat tttataggtg cagaagtagt gccctcggca tgtggtgtgc ccgatggtaa
     5221 tggcggatat ttgcgtgagg tttatattgg ctatcaagat acaatgataa aggcagtttg
     5281 ttatggagga tgttcaggcg catgtcctcc taattctcaa aaggaaatta cttttaaagt
     5341 tgatatgtcg aatacgacaa ttggccccgg tggacctcat gttataggca atttcaataa
     5401 ttacgatgtt acagcaactc caatgattga tggcggtaac gggctttact acgcggcagt
     5461 ttttgttgat acttcgcaaa caatcattta caaatttttg aaagactctt cgcttgtgga
     5521 tcaggaacaa gtacccgtag cttgcgatga tgcaaatgat tttcggcagg tttatatggg
     5581 aaacactgga cagatagtgc ctactgtttg ctacggatcg tgcactacat gtaagcaagg
     5641 tcaaacagcc gatgtaactt ttcgtgttga tgttggacaa tcgggattcg gttcgggagt
     5701 ttatttagca acaagtgaca atggtttcaa tcctttgcaa atgaatagtt tgggcggaag
     5761 tgtttattct ctaaccctaa acctcgacac cacactttca ctcgattatt attttataaa
     5821 taatctggga cccggtggtg atgaaattgt gccttcggct tgcggtgttc taaacggctt
     5881 tggaaattat taccgccatc ttgaagttcc tgagtcgcct ttgttattgc caaatgtttg
     5941 ctttggcgaa tgtgtattgt gtccaccgcc accgttaagc gatgtaacat ttcaggtaaa
     6001 tgtttctaat acaaatattg gtgcaaatgc catgtacctt gctggtgatt ttaatagctg
     6061 gaatacaact tcattgctga tgacaaatta tggaaatggc atatggaaac gcacagttac
     6121 gttagataca accaaaacaa ttcagtatcg cttttttgta ggcaatacat ttgccggtag
     6181 cgaaactgta aactcagcat gcggaattac cgacaccgta ggaggtttaa gtcgtcaact
     6241 tgaagtgccc gaaacggatg taattttgcc cgcagtatgt ttaaatgaat gtgcaaactg
     6301 tccggcaaat gtaaatgtaa ccttccgttg caattttgcg caaacaacca taggtgccgg
     6361 aaagcctcat ttggcaggta cctttaacgg ttggagttta acagctactc caatggattc
     6421 aattggaaat agcatctacg aaaaaacatt aacgcttgat tctacattaa cttacgagta
     6481 taaattttta agcgacactc ttacaactgc atacgaaata gtacctaatg catgtggaat
     6541 taataatgga ggagtttaca accgtaagct tattgctcct gaagttacta ccgttttacc
     6601 ggcagtatgt tatgcgagtt gcaacccttg tgtattcccc gactcggttg atatcacttt
     6661 taaagtggat ttatctttgg tgacacttgg aaatggcggg ccacatttag ttggttcgtt
     6721 caataactgg aatgccaata aaaatcctct atcgcaaatt accggaaata tttggggtac
     6781 aactttacgc cttgatacta ctgaagttat cgaatataaa tttactaccg ataacaataa
     6841 tgcaagtcag gaattggtgc ctgtattatg tggtactccc aatgtgtttt atggtttagt
     6901 gcgtaaactt atcgttccgc aagttgacag tactttgaca actgtttgtt tcagcagttg
     6961 tgttgcctgt cctctgcctg ttacagctaa tgttaggttt agtgtaaata tgttgcaagc
     7021 aacaattgga gccggaaagc cacatttaac aggcactttc aacggatgga gcaataccct
     7081 cacacctctt gattctattg gtaatggcat ttatgaagtc acattacaat tagataccac
     7141 atcaacaatt caatataaat ttttgaatga tactacgttt aataccgttg aaaccgttcc
     7201 tgttttatgt ggtgtgcccg atggctttgg tggtttcaat cgcagtttag ccattcccga
     7261 aagtgatatt cttttaccaa cagtatgtta ttctgcatgt accgattgta tatttcctga
     7321 tagtgttgat gtaaccttcc aggtcgattt agcgcaggtt acccttggcc cgggtggccc
     7381 acatttggta gggaatttta atggtttcga tgtagaagca accccacttg ctaatgtaac
     7441 gggaaccata tacgcagcta cacttcggtt agatacaatg ctcaatgccg aatataaatt
     7501 ccttgtagat acttcctata tcaatgagga aattgttcca caggcatgtg gcatctcaaa
     7561 tggttttggt ggttttaacc gcttgacctt tgtacccgaa aatgatactg tgctgccggt
     7621 ggtgtgttac tcaagttgtg cagcttgtgt agtacccgat agtgttgatg taacatttca
     7681 ggtcgattta gcacaagtaa cgcttggcca gggtggcccg catttggtgg gctccttcaa
     7741 tggcttcgat gtaaaagcaa caccacttgc caatgtaacg ggaactatat acgcagccac
     7801 tgttcgtatc gatactactg caaatgtgga atataaattc ctggtggata cttcttatat
     7861 aaacgaagaa acagttccac aagcatgcgg ggtgccaaat ggatttggtg gctataatcg
     7921 tttcttatca gttcctgaaa atgatacagt actgagtgtg gtttgttact caggttgtgc
     7981 cggatgtgtt ttgcccgata gtgtacttgt tacttttcag gttgatatgg cacaggtttc
     8041 gtttggtact ggcggccctc atatagtagg tacattcaat aattgggatg taacagcaac
     8101 tccattgaca tttataggtg gaaccattta tggcaagaca attttgcttg acactaccga
     8161 ttatgtagag tacaaatttt tagtcgacag ttcctatatc aacgaagaaa atgttcctca
     8221 ggcttgtggt gttcctaatg gatttggtgg atacaaccgt ttcttatctg ttcccgaaaa
     8281 cgacaccaca ataaaggagg tttgctattc atcatgcgca ccatgtgttc cattcggact
     8341 ggtaaatgtt acattccgtg tagatatgaa gaatgcacca ttaggacctg gtggacctca
     8401 tttggttggt gctttcaaca attggaatcc aacagcaaca ccgctcaata atcttggaaa
     8461 taatatctat ggtataacct taccgttaga tactacaaag gtttactctt atcgattctt
     8521 gtccgaaaat tcttataata atacagaggt tgttcctgca atttgtggta ttgctgattc
     8581 tcttggagtg ttcaatcgct ttattgatgt acccgaattt tctacaattc ttcctatagt
     8641 atgttatagc gaatgtggtc cttgcacacc tacagcaaat gtgcttatta cattccaggt
     8701 cgatcttgca caggttacac cagtatcacc acatttggtt ggttcattta atggttggaa
     8761 tgcaactgct acccctatgt ggaattttgg tggcacagtt tggggtgtac aacttccatt
     8821 ggactccaca ttaatttacg agtacaaatt cctggtagat tcaagttatg ccaacgaaga
     8881 aactgtaccg gcagcatgcg gagttcctaa tggacttggt ggattcaacc gtcaggtaat
     8941 agtgcccgaa gcaagtacaa tattgccttt ggtttgttat agttcatgca ttggttgtgt
     9001 aaataataat gtgaatgtta cttttaaagt ggacatgcat ggcttacaaa tattaagtta
     9061 caatcctatt ggtctgggtg gtgtgcatat tgttggttcg tttaacggat ggaatacaac
     9121 ggccactcca atgaccgatg caaacaacga tagtatattc gaagtgacaa taccattgga
     9181 ttcaaccact acggtacaat acaaattctt gcgcgatacc aactacacac aaagcgaccc
     9241 tgtgcctgca ttgtgcggtg ttgccgatgg ttttggagga tacaatcgtc aattgcagat
     9301 tcctgaattt gatatcacat tacaagaagt ctgttatggt ttctgtagcg gatgttttgt
     9361 aggtgtacaa gatttatctt cttcaaattg ggtggcggta tatccaaatc ctgttgacga
     9421 aatattgaca atagaattgg acatgaacaa tagtggtaat acaaatattt tattgtttga
     9481 tgcaaaaggt gcattggtaa aaaatgtaaa cgctgcatct gcaattaatc gtttggatgt
     9541 atcagcactc gatccgggaa tttacttgtt acaagtaatt tctgaaaatg gaattgtaaa
     9601 caaaaaaata accgtgaggt aattctttaa tacttcgctt aaatgaaaag gctgcttaat
     9661 tttttaagca gcctttttta ataattcact gttcattact ttttatttac aggccaattt
     9721 gaaagctcat agcgaaacgg tttcaatatt tttgaacaaa tattttttat cgatgaattt
     9781 gaaaaatagc attgttgggt tcgcaatggt tttgcttact acgaaattgt tttctcaaac
     9841 acctatcgtt gccgatatag gaactgttgc ctcggaagaa gcaagcagca tagtgcaggc
     9901 aagcgatggt ggctttgctt tgttgtttaa tggatctatc aatccgccaa taaataatct
     9961 tacaggttgt acatttttgc gcaccgattg caatggaaat gtattgtttt ataaaacgta
    10021 tgcgcccggt caggccaatg caggttatca ccttactgca acaagcgata atggttttgt
    10081 ttttcttatg gccgtaaact caaacaatat aaactcaact tatattacaa agattgacag
    10141 tgccggcaat gtaaaatgga gcaaaataat tcctgtgcgc agtggcaata tggatggttt
    10201 aagtttagat atagctggca atatttatgt tgtggcaaac gcatccctga ccaatgttac
    10261 aaaagatgaa attgcattat tgaaactttc gccatcgggc aatctgcttt tcagtaacaa
    10321 gcttgaatat actagcaact tgcgggcaag tgatatttct atttcaacat caggccaaat
    10381 ttatatttta gccatggcca atgtagtttc aacaacgtat accgatatag tattgattgc
    10441 atgcaatgcg atgggcgctg ttacgaatgc gaaaacatac actactgcat tagatgacga
    10501 acctgcacaa atgctaatat ccaacaagaa tgaattagtg atttgtggcc gcagcttttt
    10561 tagtaattca gcttatgatg gttttgcgtt gaagtgtgat agtaacctta gttatgtaac
    10621 atcaaaatat tatgatgcat caacacagct tggagaaatg tggcgcacca ttttttcgga
    10681 tggaaatagt ggctggatag tggcaggtga caaaggtgct gccggtggac gtgattttat
    10741 ttgcgccaac ctcgatagta atttcaatat ggtgtggagc aattattacc ctatagcgcc
    10801 acagtttacc aattatactt atgccggatg cagaaacata aatggctcaa ttacactcac
    10861 gggcgacttg cggtcaacgg ctaatttaag gcagacattg tttttaacca tcgctaatag
    10921 tggtaatgca ccatgttcta cacaagtgtt gccaatgact actacctccg aagttcccgc
    10981 agttcttaat gtttctattg ctcagagtca gattgttttt acccccatcg acaatatgcc
    11041 tgctatggca accataaacg tatacagtaa tggtatatgt ggtgtaaaca attcgtgcct
    11101 caattttaca acatcaaaac aaggcgactg tcctgcacag tgctatgtaa taacaaatac
    11161 aagcagtgct gcaccgggtt ctttttggta ttgggatttt gatggtggcg atccggcttt
    11221 ttcgttgcag caaattcctc ccgttgtttg ttttaaggaa aagggcgttt acaatattaa
    11281 acttaccgtt actaccaatg gtaaatcgaa tagtgttgag cgcgaaataa ataccgaaga
    11341 agattgcgct attaccattc caaatatttt tactcccaat ggcgacaatg taaatgatgt
    11401 gttttttata aaaaatttac ccgataattt tttgctcact gtggtaaacc gttggggcaa
    11461 cattgtgttt gaaacaaagg ataaaaataa attttgggat ggaacaaatc aaaaaggtac
    11521 atttgtaagc gaaggagttt atttttacat tttgcaaagc gatactttta aagagaatta
    11581 tcgtggtact gtaagcattc tttattaatt ttttattatt attattttga aattaaataa
    11641 gccagacaat tttcgcagca acaaaccttc cattttattt atacatggtt ttgccagcaa
    11701 ccaccatgtt ttcgatacca acattgcagt actgactaaa aatttcaatt gttttacctt
    11761 agatttattt gccggtattc attcatcggc aactatgcct aatgtgcatg tagaacattt
    11821 tgccctccaa attgtagagg aattgaagaa actacaactt gcatcgctca ccatttgcgg
    11881 acattcgtac ggagggcaaa tcgctataca tgctgctgcg catagcccgg gcttaattga
    11941 gcaattaata ctcatagcac caagtggtat cgaaacgttt aatcaatttg aacaaatgat
    12001 ggctcagcct tttattccct gggctgcaag gcaaataagt ggaaatgcgg aggatttttt
    12061 gcgttttgct tttcgcaatt atagcaaaga tgcccacgca ttcgtttcca aaatttcggc
    12121 tgattatatt tcgcaaacgg gcaaacatat tcaatctgtt accacaaaag tatttgccga
    12181 aattataagc gctcctactt ttagcttgct acaaaagata acaaaacctg cgttgataat
    12241 ctttggtgaa gtggatcaga ttattcccaa tatgatggca catccatttt tgagtacacg
    12301 cattattgcc gccaatgctg ccaaacaact gccatgcggt tcgtttaagt tgataccaca
    12361 ttcgggccat tttgtaatgt gggagcaagg tgcattagta aacgagttga tttacaaatt
    12421 tgcgtataca agcaccgtta ttcaacccag tctgcaataa ataaatttgt ttcgcttgtg
    12481 ccgttgttat ttcggttact gctgaaaact atttttttac catcgtgcga aaacattggg
    12541 aaagcatcga aagaagtatc gaaggtaatt tgttccaaac cggtaccatc aatatttatc
    12601 atgtataaat taaaaggcaa tcctcgtttc gagttatagt ttgaagcaaa aattactttc
    12661 ttgcccgaag gatgaaaaaa tggcgcccaa cttgcgtttg ccaaatcggt aattttttgc
    12721 attccacttc catccacatt acagatatat aaactcatca gtgttggcat taccaatcct
    12781 tccttggcca aatcgatata tgttttttga tcttcctcgg tgacggggcg cgaggcacgg
    12841 aaaattaatt tgctgccatc gggcgaaaag aatgcaccac catcatagcc caatttgtcg
    12901 gttacttgtt ttaaattaga gccatcggtg ttcatagtcc acaattctaa atcgcccgag
    12961 cgtacactgg taaaaacaat tttatcgcca cgaggtgaaa cagttgcttc ggcatcgtaa
    13021 ccgggcgaat cggttaggcg ttttatgata tttcctttaa ggtcgctaac aaaaacatca
    13081 aagtccggaa aaacagccca cacatatttt ccatcggttc gttttgcagg tggagcaggg
    13141 caggtctctt ttcctaaatg cgtagaggca aacaagatag tggtatcgcc aggcaaaaaa
    13201 tacgaacaag tagtacgccc tttacccgtg cttaataagt gcggtatatt tttttgtaaa
    13261 tcatctttac gccaatcgaa ccagtaaatt tgatcgcact ccagattcca ttgtttattt
    13321 gtaacctgaa atgtaagtaa gctatcattg aaactaaaat aggcttcagc attgtcgcca
    13381 ccaaaggtaa gttgctttac atttttgaga tgaacttcct tattgtattt taattctaaa
    13441 ggtaaccaac ccgggttttc ctgacctatc gaattattta tagcgacaag cataaagacg
    13501 atgaaaaaat attttagcat agtgagattt tatttgctgc aaaattatac ttcttgttct
    13561 attataaagt gaattttagt cggttaattg taatattata atttaaaaaa ttcaggtttt
    13621 ataaaacaaa tagaatgttg gtaatttatt ttttcgaaac tttagttgcg ggtacaagaa
    13681 tttatacctt tacgctaaat atttatatta acttctaaaa aattgatgat gaaaaaagta
    13741 ggaattattg gatcgggtaa tgtagggaaa gcattatcac atggcttttt actaaacgat
    13801 tacgaagtaa tgattggcac gcgtgatacc catgcattgc ttgaatggca agttgccgaa
    13861 ggtgcaaatg caatgcccgg cagcgtaaaa gaagccgcag aatttggcga catactcgtg
    13921 cttgccgtga aaggcgatgc ggcaatggct gtgatggaac aattggatgt ggcatcttac
    13981 gcaggcaaaa caattataga tactaccaat ccaatcgaca attcgaagcc acctgtaaat
    14041 ggagtattgt cgtattttgt cagcggtagc gattcgctta tggaacaact acaacgaaaa
    14101 tttccacaag cgaattttgt gaaagctttt aatagtgtcg gtagctcgtt tatggtaaac
    14161 ccaaagtttc cggaaggtaa accaaccatg tttatttgtg ggaataatgc cgatgcaaaa
    14221 aagaaagtag aggaaatcct cactgcattc ggatggggaa ctcaagactc tggcatggtg
    14281 gagtcggccc gttctattga agcgttatgt cagttgtggt gtgcaccagg atttttgcgc
    14341 aacgaatgga ctcatgcctt caaactattg aaaatgtaaa ttcttaatta ttgcattttt
    14401 ttcaatagca gcgttttgtt tggtttgatt ttctacaaaa tactaaaccc aacttgcttt
    14461 atttgaagaa aatatttcag tcaatatttg tacatatgca tgctgaagaa taaatttatt
    14521 ttgaacaagt ttaaaaaaaa ataattttgc caaaaagaat ataataaaca taaatcatta
    14581 aatagaaaat agatatgaaa cactttgata aaattctttg cccatacgac ttctcaccac
    14641 attcggaaga agcactccgt tatgctatga agctggccga taacactact ggtattacac
    14701 ttcttaatgc tatacagctt ccttatctta ttgaccctaa tgggttttct tattatgatt
    14761 tcaaagcgga tgaattaaaa aaatctactg aagactctct agccaaaaaa gtagaagaag
    14821 taaagagtac ttatcccgat ttaaatttcg atttcaaaat tgaagtagat aatgatcctg
    14881 cggagattgt actcaaagtg caaaaagaag ggaagtatga tcttatagta attggttcgc
    14941 atggccgaaa aggccttggc cgtatattga tgggtagtgt agccgaagca atattgcgcg
    15001 atgccgattg ccctgttttg ataataaaaa tgattaagcc caaaaccgcg gcttaaataa
    15061 tggtattttt tccgattaaa tgcattaata ttaaagttaa atttaaaata aataaagaca
    15121 aattatgaaa gacagtattt cacatttgca tgttgtgtgc gaagattgga tgagagaatt
    15181 acagttttac aaaaacgaaa ttcctttttt caaaaaacgc atagaagaat tatctacaaa
    15241 atatactacc aaagaggtgt tggcacaaat agaacatttc gaaaataaat ttcacattat
    15301 ggatacacat tgcgatgagt tgattcatga tgtaaaagca aaaaataaat ggttgcttaa
    15361 tcaggcaagc gaacagccaa aatttattgg agttaaaatg atagcagccg atgaaaatat
    15421 tcccgatctg atggagttta cagctcgtga ttttgcatca acaaaaaagg agttttatga
    15481 gtttttgagc aagtacatgt aagctgattt attgcatttt ttatttcttt aaaaaaaatg
    15541 gttgtgctta caattgagtg aagcaactaa tcaaaggttt gtgtacggga taatttctga
    15601 aaaggaatga aagatgaagc aaaagataaa tgagtatgag tccctataca taaatatttt
    15661 atcaaattgc ttctggcacc aattatcgtg tggaaacttt agtcaaagaa actcattcgg
    15721 atttatatac aaatatttgg aggagtagaa gttcaacccc tatcggggtt tggtgtgtgt
    15781 ggggtttgca gtttctccgc gtttcacacg gagttattca tatttaagcc cttcaggctt
    15841 ttcaaatcga atcataaaac aacagcaatg agttaccacg aagtggttaa acatgaataa
    15901 ccacgaataa aatttgggga ctgaaacaaa tgacggaagc ccaaccacga agtggttaaa
    15961 caataatatt cttcgacatt ctatttgggt agagaattga aatcattaac aagtatgtaa
    16021 atcattaatt ctaattttca atattttcac ctttttgaat ttgaaatagt catgactaat
    16081 aaattcattt cttaatttta tccatgcgtt ttaacgaagc accaaatatt tctttaaaca
    16141 tttccactca ggaaattata aatggcaatg tgagtttagt aaccaaataa acaatagcga
    16201 tacaaataac atcgagccag aatcccacgc ttaccatctt acgaataggg atataccccg
    16261 agccaaatac tatggcgtta ggcggtgtgg ccataggaaa cataaagcca acgctggcag
    16321 caagtgttac aggtataccc atcatcaatg ctttgtgact atcgttgccg gcaatgccaa
    16381 ataccacagg tataagcact tgtaccaaag caacattgct cattatttcc gataacatta
    16441 ctgtaataat aataagtgca atcataagca catctaatga aagattttga cttagtacat
    16501 aattgccgat aaattgaaca atgcccgttt tttctaatcc cgaagcaaga cataaacctc
    16561 caccaaatag tagtaaaatt ccccagggca actttgctgt atcttgccag tgcagtacga
    16621 attcaaattt tttgaaatta acaggaatta taaaatataa tatggcgcct cctatggcaa
    16681 tgtttgtgtc gttgagtatg ttgcttccaa ataaattatt taaaggttgt tgggtaatcc
    16741 acaaaaagga agtaagacca aaaatagcaa gtacaatttt ttcggtcttt tgtaaaggtc
    16801 cgagcagtag tagtttttcg tgaataaaat tggaagagct ttgccgcgct tgatttttta
    16861 cacgatacat aacttttgta atcaggaaat aaaacaatgc agaaataaaa atggtagctg
    16921 gaaaagcaat tttcatccat tgcaaaaagt ttattttctg atgataaaac ttttcgtaaa
    16981 ggcctacaaa aactacatta ggtggagtgc caataatggt tgccatgccg ccaatgctgg
    17041 ccgcatatcc tattacgagc atgaggctaa gcgcgaaatt tttatcgtct ttgtttagtt
    17101 gaggcttttc ttttgtcttt gatatttcaa tcaccgaatg tgctatgggt agcatcataa
    17161 gcgctgtggc tgtgttgcta atccacatac ttataaaagc agttgcaata agaaatccta
    17221 aaataatacc attacttgat gtccctgtaa tatgaattat tttgtatgca agcctttcgt
    17281 gcaaaccatg ccgctccatg gcaatggcaa tcataaagcc accaaggaat aagaaaataa
    17341 cactgttgcc aaatgacagt gaggcatcgc tcatgctcat aactccggtt gcaggaaata
    17401 aaactaaagg caacaaagca gttaccggca atggcaatgc ttctgttaac caccatatta
    17461 tcattaagct tgcaatagct aaaaccttgt atgcatcggg atgaatggaa ttgtgcggaa
    17521 aaaataacca tatgaaaacg aaaacgattg gcccaagaat aaaaccggtt tgcgataatc
    17581 gtaattgaaa agtttcctgc atattatttt tttagaaatc gcctggtcat attcatttta
    17641 ctgctggaaa tttgaaccga aaaaattccg cctgataatg tttcgggcaa agttattttt
    17701 tttgaattac aatttgattt tatctccatc accactcttc ctattccatc gtatatggtt
    17761 acagtatatt catccaataa attttccggg cagttaagaa acaaattact ttctattccc
    17821 tcataaacaa tttgaatact gttttgtgtt atatcgttcc atcctacatt tgcagctatg
    17881 ctcagtgaag gcacttcgca cccgtttgaa tctgtaatca aaacatagta agttcctgct
    17941 gtgaagggaa cataaaaata atttgtacca agtaccaggt ttgggttaag tgtgctgtac
    18001 catgcgtaac tcacattggt ggcagtacaa atgagtgaat cgttttgcca taaaatagtt
    18061 ggtgcagcgg ggctttgaaa ctctgtaatg aaagcaggaa agaaaacagt atcgcatccg
    18121 gcattgttgc aggcaattaa ttgcacatca aaggagccat aattattata gcatattcct
    18181 gcaggatttt gcattgtaga agtagaagga gatccgcctg taaattgcca ctgccatgtt
    18241 gttggactgt tcaccgaaag atcgtaaaag tcgatgcatt gtttttcgca aaatagggtg
    18301 tcgctcgagc ttaaattaca ttggggaata tttccgcaaa aagaaatagc gcaagttgta
    18361 tctatcacaa atttccacaa agtatttact gcattataat tattgttcca gcctcccatc
    18421 atccataacc catcgctgct gtccatccaa cttaccgcgc ccatgagtcc tcccggttca
    18481 ttgcccggtg ctgctatgcc ttgtgtgcca aaaaccggtg ctgtgaaaac agaaggattt
    18541 ccatttatta aagcccatga gttgcacttt ggattataaa cccataaatc gctcaggttc
    18601 gacccgccat aattccagaa gcgaccttgc ttatcaatcc acgatgcctt attctcgaat
    18661 cggaactttg gtaaataact tgtgtcggca ttacagtttg gtccgtttgt gccgacatca
    18721 ttaagcacat ctgtccctcc catccatgtc caaaatcctg tagtgctatt gtacatccac
    18781 atatcgttga aacttcctaa agttcttgca ccggcaaaca tccaaaagtt cccatcgcta
    18841 tctttccaat gcgaatgcgc ctttcggggt gggggttcgt tggttgccgc aggtacaccc
    18901 tttattccat agtttccgtt gctgtttgta acgctacttc cactaaccca tgtccattcg
    18961 cctgtcgata tctggtattt ccacaaatcg ttaaagtcgc cagttgtggt aaaaccgcca
    19021 aaaagccaaa gttggttact gctatcgacc caagctgcat ttgtttccca gcggctaggt
    19081 ggttggtttg ttggtgatgc tattcctttt gtgccataat ttccaggtgt acagcaagtt
    19141 gttgaacctt cttcccacga ccacatgtta atagctggat catatttcca caataaatta
    19201 tagctggtac ctccaccatc gctgccacca aaaatccaaa aattacctgc agtatctacc
    19261 catgcagcag cacaccattg ctgattgccg gggctattgg aaatggcagg aattccctga
    19321 gtgccataaa ctggccccgt gaatgtattg cccgaccctt tcatccaagt ccattcattt
    19381 gttgccggag tgaatttcca aaggtcatca taaacccccg tgttgaatcc gctgaaaaac
    19441 caaaaatttc ctgccttatc ggtacccatt gcaccttcat agccaccagc cggagagttt
    19501 agcaccgagg gtacgccttg tataccatat actcctgagg gatttgcatt tgtacttccg
    19561 ttcatccatg tccacattcc ttgttgcgaa tgcaatgaat gatcagagaa aataagtatg
    19621 cataacaaag ttaattttct cattgtttca atttataatt ttacaatgcg cttttcaatt
    19681 tttgttttat tgttggaaat agtaataaga taaaatcccg gcaaaaatga ggatggtaat
    19741 tttattgaca attcgctgca attaaaatat tctgtaaaaa catttctccc aataccatcg
    19801 tatacagtta tggtataatc atccgtatta aatccattac aatttattag taaactgttt
    19861 tctgtattgt cgaaagaaat ttgaatgttg ctttgtgtta aatcattcca tcctacattt
    19921 gcaattactc caatggacga aacatcacaa ccattggtat ctgctatcac cacataataa
    19981 gtgccggcaa tgttgggcag ataaaaataa tttgtaccca atattgcatt aggattgagt
    20041 gtgctgtacc atgcataagt aacattggtt gaagtgcata ttaatgaatc gttttgccag
    20101 attaccaaag gtgcaggcgg actttgaaat tcgctgataa aagatggcag cagcaaagtg
    20161 tcgcaaccaa tattgttgca ggcaattaat tgcacatcga acgaaccata attattataa
    20221 caaataccgg gaggattttg caatgtagaa gaagaaggtg atgcgcctgt aaattgccac
    20281 agccaagttg ttgggctgtt tgtagaaagg tcgaaaaaat cgatgcattg tttttcgcaa
    20341 aaaatagtgt cggtgcacga gaggtttgca acagtatttg cacaaggtgt aaaagtaaca
    20401 tcgatactat cgcttgcttt gcaaccaaaa ttgtcaacag taatagcaat ggtagaattt
    20461 gcagttacac tcaacgaatt taatgtggta ccattgctcc ataaataaga attgtaaatg
    20521 cccggattaa gaactaaagt ctgaccaatg cataatgtag tatcgctgcc aagtgatatt
    20581 gtgggagttg ggttaatggt gatgggataa taacttgtat cgacaaacgg aggcacagtg
    20641 agtgcacgaa ttaaagtaac aacataattt cccggagcag tataaacgtg agaggtattg
    20701 gcataataag aaatgttatt gggccccgaa gaagggtcgt caaaattcca ttgaaatgct
    20761 acgtagccaa tagaatcttg cgcaagaaat aatgtagtat cgccaaagca aaagtttttt
    20821 atttcaatgg catcattgta ggttacaatt gccgcccata tagaattgtc gacaccgtta
    20881 ttttgatgat tgtattgaac aaatatacta tcgctgccag gagtgactaa gccatttata
    20941 ttactcaaaa cttcggggcc tgttatggct tggtttgcaa tgtcatcatt gaggccggtg
    21001 agtattttgt tttcataata aaaacttgca gcaggaccac cacaactgcc aggcaggttg
    21061 tttgcatcgg ggccataagc caaccctatt ggcgttccat ttaccgcaac attttctcca
    21121 tcgcccgacc cacacatgta tcccattaaa aatgcaagtg caacatcgcc cgatgccgga
    21181 aatttatttt gtaattttaa ataatgacta acactctgac ccatagcagt tgtgtttaaa
    21241 aatatggccg aacttataga aggtaaggct ggattagcat atgcaacata caaatagaaa
    21301 tcggtaaaaa aatcaacagt ggtgttcaat tgtattcctg taaaagtata aacagtatcg
    21361 gtagggatga ttagcgatgt aacatcaata gcatgcactg ctgcactatc gccataaagt
    21421 tgattgccaa acttgggcga tacctgtgtt acgttatgga taaaagaaac ctgattgcca
    21481 tcgagcaaaa aattggccgt gctgccatcg cccatttcgc cacacatcaa gaaagcctta
    21541 tgaattgtgc tgcccggagc aatatgaatt tgaaacactc cattcgcgtt aggacttaca
    21601 ccaggtgaat atcctgcaca agtaacaccg ccattaaatt ttgtttcgta aaattttgtc
    21661 tgcgaataac ttttcgtaac gcatattaat aaactcagga aaatgtaaaa ggtaatttta
    21721 tttttttgca tgtgaattaa ttttgatttc aaagatagaa aattcttgtc attggatgtt
    21781 gtatatggtt attctgttaa ttggttatag cgtaaagaaa ttgtaacgtg ttttattttc
    21841 ctcactttta tgcgaaggcg gattttattt tcttcacttt aatgcgaagg cgaattttat
    21901 tttcctcacg tcattgcgag gaggaacgac tgaagcaatc tcagcggggc gtgcattttt
    21961 cgttgcagaa attacttcgt tccaaaacta ttcggaatag taataacgga aatagccaaa
    22021 gacattattc aaaatgacca atgaccagag tttaaaatga ccattgacaa gaactcaaaa
    22081 atgaccaaag tctggcagag attgcttctt ccgatagtaa tcggaatcgc aatgacggaa
    22141 atgtccaatg acaatcccca atgaccaatg accggtattc aaaaatgacc aatgaccgtc
    22201 cacaataact ggcagagatg cttcgtgcct ctgcattgca aacaacccaa caactaatca
    22261 atccaataac taaataacta atcaacccaa taactaaata acccaacaac aaaataacca
    22321 atccttaaag tcccaataat ttcgattgca aaatgtaggt ggcaataccg gcaaaaaatc
    22381 ctgcagtagc aagccacgat atttttttaa ggtaccagat aaaattgatg ttttgtaaac
    22441 ccataatagc aacaccggct gcagagccaa taatgataca actgccaccg gtaccggctg
    22501 taagtgccag gaattgccaa aagtaatggt cggtatgata agtagtaagg tcgtacatgc
    22561 cttgtgccgc tgctaccaaa ggtacattgt cgataacggc tgatagcatg ccaagcacag
    22621 tcccgattat atattcgttt tttaaagcag tatcgagata agaagcaagc gagtgcaaaa
    22681 tgcctaattg ctcgagcgat gaaacagcaa gtaaaattcc gaggaaaaat aaaatacttg
    22741 gagtatcaat tctttgtaag gcttgatgca aactaaattt atttcgcaat gtttcttcct
    22801 tgcgcttgtg taattgtgat gtaagcaccc acataaaacc aagtgccatc atcatgccca
    22861 taaacggagg catatgtgtt acagttttaa aaattggcac aaaaatcaac atcccgattc
    22921 ctgcatataa cacccatttt ttttcggatt gataatcgtt atttatttca tgatttacca
    22981 ttggaatagt ttgccctttg aatttagaca tcatcaataa cattggtaca ataagcacaa
    23041 caaggcttgg caggaaagtg taacgtataa tattgcctgt agtgatttga ttacctatcc
    23101 aaagcatagt ggttgtaaca tcgccaatag gtgaccacgc accgccagca tttgcggcta
    23161 ttattatcaa gccggcaaac caaagtcggt cctctttatc tttcaataat tttgtacata
    23221 acgatgacat tactatagca gttgtaagat tatctaaaac cgctgaaaga aaaaaagtaa
    23281 gcacaccaat gatgagtaat aatttcgatt tgctgcgtgt tcttattttt tgtgtgatta
    23341 catcgaagcc gtgatgcgag tcgatgagct ctacaatcgt catagcacca agcagaaaaa
    23401 acaggatgcc ggctatatcg ctcatgctat ggtttaagtt ttctgttgcc acatgcatat
    23461 ctgtagcgcc aagaaaatat atcaaccagc aaatagtgcc gatgagcaat gccggtgcag
    23521 ctttgtttac gtgtaaattg tgctcgaagg ctattgctaa ataacctaca ataaataata
    23581 ccactgccag aataaccata actgtatact aagtttaaag tgagatttta attataattt
    23641 gaggttgtaa ataaaccatt actaaattta agtgcaaaat catgaaatat ttagttggtt
    23701 ttattcatca aaaattttgt tttagaattt ccatttgtgc aatggtttca ttgataatat
    23761 catcaattat ttgtgcacac ggttttatgc tatgcacaag ttcggccgat tggcctgcac
    23821 tccagaggtt gttgtaattg cccggcttta tggctttctc cagatttttc atgcctttgt
    23881 attgtaccag tgttttgaaa tattttttcg tagttgcatt tgtattcaac catcgttcga
    23941 gccagttttg cttgtagcct atttttttgg cgtaaggtgt atcaattata ttgcaaggtg
    24001 taccactcac tcgttcagtc atcacaatat tttccatgcc cgaatcaaca atggcatttt
    24061 tatattcctg cgatacggtt gcttcttctg atgcaataaa tgcagtgcca atagaaaccg
    24121 catcggcacc acatgccaaa gccgacaaca tggtagcacc atcaccaatt cctccggcag
    24181 ctataacttg cagatgagga aactcgcgtt tcaaagccgg cactaaaaga gataatggat
    24241 atggccccgc atggccgcct gcaccctgac ctacagcaat aaaaccatct atgcctgttg
    24301 atgctgcttt gcgtgcatga gtcatattgg tgacatcgct atatactttt gctccgtaag
    24361 gttttacggt atcaactacc tgcgatggac tgccaagtga agtaatgtag acaggaactt
    24421 tttgtgccgc acatattttt aaatgctgct cgaaataaat gtttgttttt tgcacaatta
    24481 aattcacacc ataagtgcct gcgcaatttg tgtcagtttt gtattttgaa attgtctgca
    24541 gcgcattggt taaagcagca tcatcgcgaa aatttaatgt aggaaaagta cctacaaccc
    24601 cgcctttcat ggcttcaact atcatagcca cattcgaaac caaaaacata ggagccataa
    24661 ttattggggc tcgcaattgg agttgcctgc tgaaagctgt atttattgtc gaagtcattt
    24721 gatgtgcaag tatgattttt tatttttcat atcccaagtt tttgtaaata atatttttct
    24781 attttaaccg cttcaatttt cacctcaata attaatttta aaattatgac agaagtaact
    24841 cgcttattcg atgttgttcc ttatcaactt gaaaaatttc ctaaagccga ttcgcttgca
    24901 ggcaaagata atggtgcctg ggtaacatac agcacacaac aatttgccaa ccgcgctaaa
    24961 gaaatagcga tgggattaat agccctgggt ataaaccggg gcgacaaagt tgtgtctata
    25021 agtaataacc ggcccgagtg gaatatgctt gatatcggta ttttacaaac aggtgcagtg
    25081 catgtgtcca tttatccaac catttccgaa tcggaataca aatttatttt gaacgattgc
    25141 gaagcaaaat taatcgttgt aagtgacgag gcattgtgga aaaaaatcga ctctataaaa
    25201 gcagaggttc ccacactgca aaatttgtat accttcgaga agatgttggg caaaatacat
    25261 tggctcgaaa taattaatgc cggcaaaagc agtgatgaaa gtgtatggca aatgagaatg
    25321 gatgcagtaa tgcctaccga ccttgcaaca cttatttata cttcgggcac cactggaaac
    25381 cccaaagggg tgatgctttc gcacagcaat ctggtgagta attttattgc ctgccgcgat
    25441 ttgcctcctg ttgattcacg ccatcgtgca ttaagtttct tgccgctttg ccacgtttac
    25501 gaacgcatgc ttacttattt gtatatgtat attggggtgt caatttatta tgccgaaagc
    25561 atggaaaaaa ttggtgacaa tattcgtgaa ataaaacctc acgttttttc ggccgtgcca
    25621 cgtttaatag aaaaagttta tgaccgcatt atcaataaag gaaaagaact tacaggtgta
    25681 aaacgtgctt tgtttttttg ggctgttgaa ttaggtttga aatacgaaca tggtaatcga
    25741 aatggaatag tttatcaaac gaaacttaag attgccgata aacttatttt ttcgaaatgg
    25801 cgtgaggcac tgggtggtaa tacgatgtgc attgtatcgg gtggtgctgc attgcaggaa
    25861 cgccttgctc gtgctttttg ggctgctggc atgcctatac tcgaaggata cggtcttagc
    25921 gaaacttctc cggtaatagc tgtgaattta ttaggcgaca accttgcatg ttttggaaca
    25981 gtaggaacaa taataaaagg tgtagatgta aaaattgccg atgatggtga aattcttttc
    26041 aaaggaccaa accttatgat gggatattac aagcgcgatg atctcaccaa agaagtaatt
    26101 gataatgaag ggtggcttca cacaggagat attggtacgt gggttgagga aaagtttttg
    26161 aaaattactg atcgcaaaaa agaaatattt aaaacaagtg ggggtaaata tatagctcca
    26221 cagcccatag aaaacctgat gaaagaatca gcttttatag aaaatattat ggtgattggc
    26281 gaaagtcaaa aacatgcttc ggccattatt gttccaaatt ttgcacatgt gaaaagctgg
    26341 tgcgaaatac ataaagtaac atacacaaca cccgaagaga ttataaaaaa tccacaggtg
    26401 cgcgatagaa ttatgaagga tgtagaaaag gtaaatgctc aattgggcca tcacgaaaga
    26461 ataaagcgat ctgaattaat tacaaaagaa tggacagtgg tttcaggcga acttacacca
    26521 acactcaaat tgaaaagaaa aataatagaa cagaataata aagatttgat agataaaata
    26581 tactctgtag atgacgaaaa atagcgcata ccattatctg tataaagcat ggctatttct
    26641 ttttccctgc agcaaaatat ttgctcttta ggtcgaaatg aacgactaac ttttttcttt
    26701 tatttcctat aatggagttc atcttcttgc atcatcaact ttaaaaacaa aatcatgttg
    26761 caagtttttc atcagcattt atgcgcttgt atgtcttgtt ttattgcgct gccagttttt
    26821 ttgtggcttt ggttcttgct tggcaccggc atgtggctta gtactgcctt gctgtggtct
    26881 tcctcccgat tgtgatctgc gctgatgttg tttttgttcg gtaggtatag ggtgattgtc
    26941 aatcaatgga aaagcatggt catcaatcac gggaatacgt tttgatatca atcgttcaat
    27001 atcgcggagg tatgcttttt cttcggcatc gcaaaacgaa aatgctgtac ctgctgcacc
    27061 tgcgcgtcct gtacggccta tgcggtgtac ataagtttcg gcaacattgg gcatttcata
    27121 atttatcaca tattgcaaat catcaacatc tattcctcga gctgcaatat ccgttgcaac
    27181 taaaacacga gtggactgtg ctttgaaatt ggataatgca cgttgtcttg cattttgtgc
    27241 tttattacca tgtatggcct cggccttaat gtgatgcttc tccaatactt ttaccacttt
    27301 atcggcccca tgttttgtgc gtgtaaatac caaagcggtt tttattttcg tgtctttaag
    27361 tacatcaatg agcaacgaat ttttatttcc tctgtcaaca aagtaaatgt attgctgcac
    27421 tgtttcggca gtggtcgatt cgggggtaac ttctactttt ttaggatggt gtaaaatgct
    27481 cgatgatagc tttacaattt cagggggcat ggttgcagag aaaaataatg tttggcgttt
    27541 agcaggcagc actgcaatta gtttgcgcac atcatgtata aaacccatat cgagcatgcg
    27601 atcggcttcg tcaagtacaa atatttctac atcgcgcagg gagatatatc cttgattcat
    27661 caaatcgagt aaacgaccgg gagttgcaat aagtatatcc acaccgtgct gcaatgaagt
    27721 ggtttgtggc ccttgtgcta caccaccaaa aacaacagtg ctgcgtaatt ttgtatggcg
    27781 gccatatgct ttgaaacttt cgtttatttg aatggcaagc tcacgggttg gtgtaacaat
    27841 gagactgcga atttttaatt tcttgtcatt ggtgtgaacg ccacttaaca gttgtaaaat
    27901 tggaattgca aatgcagcag tttttcctgt tcctgtttgt gcacatccca gcaaatcggt
    27961 cccttgaagt actattggta tggcttgttg ttgtattgga gtaggcgtgg tgtagccttc
    28021 ttcctgtatc gcttttagaa taggttcgat aatacctaat gaattaaaat gcatgtaaaa
    28081 attgtttgcc ggaatagtcc ggtatgaaaa tattatatca gtttgtttat aaaaagttgt
    28141 gcaatgtagc atttatttga tgtaatacca acaccagtca ttcaggagga accttattca
    28201 tacattagat aaatttgttc aaaaacaaaa tcccctaagt cacaagaagt ttcctcctga
    28261 aggactgaaa ggtgcatcgt cattcacccc ctctagtcat tcaggaggaa ccttacttct
    28321 accttagata aattagttca aaaacaaaat ctcctatgcc acaagaagtt tcctcctgaa
    28381 ggactgaaag gcgcatcgtc attcaccaga aaccttattc ctacattgca taacaaaccg
    28441 aaaaaatatt tccctacatt tgcttctatc taatctgcta atcattttaa ataattcacc
    28501 atgtcgaaaa ggataatact ctactgcttt gcatttttat ttttccaaat tttaaatgca
    28561 caaaagcaaa attcacaggg aggaaaaatt atttcaggcc caatgcttgg atacgttgaa
    28621 catcgcgaag ctttgatttg ggtgcagaca gcttgcaata aatccgtcac tataaaatat
    28681 aaaaagcaaa acgattcaaa gatttttacg aagtcaatat ctaacataaa cacagataaa
    28741 tgcgaaccgt ggattgcgaa attcgtattg ccattacttg atgtaaacag cgaatatact
    28801 taccaaatat ttttggataa tgctcccgtg aaattttctt atccgctaaa attcaaaacc
    28861 gaaaaattgt gggcaccgtg gagcactaac gatccatatg aaatcaattt tttattcggc
    28921 tcgtgcaatt atgtgaacga tagtgcatac gaccgtccgg gaaaacctta cggacaaagc
    28981 actacaattt ttaatgcaat ggcaaagtct aatgccgatt ttatgatttg gcttggcgat
    29041 aacacttacc tgcgcgaagc tgattacagc agcatttcag gtataaatta ccgatacctg
    29101 cacacacgtg tagaaaaaaa tctgcaacca tttcttgcct caatgccaca atatgcaatg
    29161 tgggacgacc atgattttgg tgacgatgat gctagcaaaa atttcgcctt tgcaaatatt
    29221 actcgacaat gttttataga ttattatggc aacaatcaat atggtgaaaa tggacaaggc
    29281 acctatttta aatttgtaaa aagcgatgca gatttttatt tgacagatgg cagaactttt
    29341 cgtgacgaaa gcaaacttga cgaaaaaata tttccgcaaa aaacaatgtt aggaaaacaa
    29401 caactcgaat ggcttaaaaa taatttggca catagtacat caactttcaa atttatttgc
    29461 ctgggcggac aatttctgaa tagcgaaacc acaaaggaat cgttcaattt atttgaaaat
    29521 gaacgtaaag aaattatgca atttattgcc gaccaaaaaa taagtggtgt aatttttatt
    29581 tcgggcgaca ggcatcattg cgaattgttg aagtacgatt cacttgccaa tcccggttat
    29641 gctgttccat atacattgta cgatattacg agttcgccac ttacatcagg tgtttcgggt
    29701 attttgaact ctcccgaaaa aaataatccc caccgggtag cgaacacact ttttgttgga
    29761 aataattatt gtaaaatgtc aatcaccgga aagcgaaatg aaagagtggt tactttattt
    29821 tgttatgata gagatggagt ggagaagtgg aagtatgaaa ttaaggagat tgagttgaaa
    29881 atgcattaac ctttttctat tgacaaatat ttcttgataa aaatatttgc caatcaattg
    29941 gtaaactgat gtatagcaat tttcaaaaat ttatattcgc gcacttttaa aaataaaata
    30001 agaatggacc aggaagattt aggaattgga agccgagtaa gccaccccga gtttggcgat
    30061 ggtgttgtta taaatgtaaa aacccgaact tacgaaattg tattcgttcg ctcaggccgc
    30121 atggaaattt cgaaatttca aaatgcactc actgttatcg aagttgtaga acccgacacc
    30181 gatttagtct cgaaaagtga aatggaggtt tcgttgataa atattttgaa acgctatagc
    30241 gacctgcaag aagtaattcc attaggacaa aaatggattg gtggaaaaat aattatgcaa
    30301 ccgggcaaca ccagtttgaa aggcaaagaa atttctatcg atgcattttt tcacaagata
    30361 gtaatgctgc gcgatcgcct tcgtgttatg gaacaacgca taaactcaca ttccataatg
    30421 accgatgaag acaaagtaaa catgcaacaa tacatcaccc gcatatacgg cagtctcact
    30481 accttcaacg atttattcaa aaacaaagaa gataattttg ttggggataa agggggagag
    30541 taggggtaaa gtcatttaga attctcgtca ttggtcattg tggttggtca tttttagttc
    30601 tggtcattgg tcatttttaa ttctagtcat ttgtgttttt aatttggtat caagttgaaa
    30661 tgctggtcat tgatgattca aaattcccta gtcaggattc ttgcaaaatt tcgaataaaa
    30721 attatatatc aacaatgaaa ttcttcgctg cccaagacaa atcataatga ccaatggcca
    30781 ttgacaaatt cgaccaatga cagtatgtct cgtcctgaaa taggtttacg cttattaaag
    30841 gaaaaaaaat gataagtaaa caaaagacaa atgaagaaaa agaggctatc atagccgagt
    30901 atttattagg tgaaaagaca tttcggcaaa tgcaatcact gcatggagtt gattatagaa
    30961 taatacattc gtgggttaca aaatttaaag gccatcatgt gcagaaatta aaaacaccaa
    31021 cagaaaccat tcaattctat gagcaacagg aatgcgatgt aaaagctttg cgagaagaac
    31081 ttcgcaaagc aaaactgtac aacgagttgc ttaatacgat gatagacatt gccgaagatc
    31141 aattaaaaat tgatatcaga aaaaagtctg gcaccaagcg gtagaaacat tagagcaaag
    31201 gagtccgcgt tgtgtaacag acctttgttt gcttcttggt aatagtaggc aagcttacta
    31261 tcagggcatt aagtacgtac agcaaaaagc gtatgaatcg catttaataa tcgaagaagt
    31321 gttgcgactt cggaagtttc agaagcgatt aggcacacga aaattattgc acgaaatgca
    31381 agattttttg tcggcccaca gttttcaaat tggcagggat gggctttttg atttgcttgc
    31441 ggagcacggc atgttagtga ccaagcgcaa gcgcagaggc tgtgttacaa cattatcaaa
    31501 acatcgttat aagaagtatc caaatcttat taaagaattc attccacttg ctccaaacca
    31561 gctatgggta agcgacatca catatatcca tttgcaaaaa ggtttcgcct atttgagttt
    31621 gattacagat gcatatagtc ataaaattgt tggcttctat ttgcattgtg acctttccgc
    31681 acaaggtcct ttgatggcat tgaaaatggc attgaaggct aatactacta ttgaaaattt
    31741 aattcaccac agcgatagag gtgttcaata ttgttgtgat gtatatgtga acctattaaa
    31801 gcacaatggc gtaaaaataa gcatgacaca gaatggcgac ccaagggaaa atgccgtggc
    31861 cgaacgtgta aatggaatat tgaaaacaga acttttggaa gataaatttg cagattttgg
    31921 caaagccaaa actgcaattg caaaggcatg tagcacttac aattatttac ggccacacaa
    31981 tagtattgaa aatttgaagc cagtggaggc tcatcaaaaa gaaggtacat taaaaaaact
    32041 atggaagaat tattacaaat taaaaaagga aaaggaggta attatggaat aggttttttt
    32101 aggcattggg tgttctgaat atatctctcg cacaaaagcc aagctgttac gcttggcctt
    32161 atagaacgat atacttcgtc acacactggt ttatttcctg gtaggttgca cctctgcaga
    32221 gcctacttcc gcttttgcca gtgatgcaaa tatagaatgt aaacggtatt caggattatt
    32281 ttaaatgtgt aaacttgcac acggatttta aacaaaatgt gtaaactttt ttcaggacta
    32341 gacagtattc aaatgacaag tattcgaatg acaaatgaca agtactcaaa tgaccaatga
    32401 caagtattca ataatgacaa tcctaaaaac catgcttccc cacactccaa cctctgctcg
    32461 ataaaaattg ctcacccgat tgtattgcac cggattccaa ttctgttgcc atagaatcgt
    32521 tccatctgtt tagaaaaccg aacaaagaaa tcacacccag aatttctact atctcaccat
    32581 cgttccaatg tgcacgcaaa ttttcggcaa tggcatcatc tactgcattt ggaattgtgg
    32641 atgcagccac agcaaaatcc agtgctgcac gttcggcatc attaaaactt tcatgcgttt
    32701 tatattccca aatattttcc aattgtgtgt gcgatgcacc ataacggtcg gcagcacgaa
    32761 tggtatgtgc ctggcaatat ttgcatcctg ccacattgct gcttatgtaa cctatcaatc
    32821 gtttcaaagc actcgtaaca tgtcctttat tttccatcac tgctttgttc aattcaataa
    32881 acgcatatgc aaggtgcggc ctgtggtgca tggtttttac actgttaggg caaaagccaa
    32941 gagtctcatt aaaaaattct actagtttta ataactcttt atcgttatca tcttttttag
    33001 gaaatacaag tggtctattc ataaattatt ttttctgcaa tttgaaattc caaatgtaat
    33061 atttttagct ttttgtttac accaatcagt taaaagttag cctcagtaat tgaagtgatt
    33121 ttataaatgc gtttcgaatt tatttgtaat atttatttcg gcaatttgtt tatttgtcct
    33181 ttagattaca tatacaaaaa aaatttatct tttgctttac acttcttaat tatcagtttg
    33241 tattttagaa attgcaagga agaagtaaat taaaaaataa aaaaaacaca caatgaaatc
    33301 aacaagcaag gaagtaaatg catttttctt atcaacaaaa aaagcaattt atgtcgtagt
    33361 attgggacta tctttttcgg caaatgccca atcgccaaaa actgttttgg tagaacattt
    33421 taccaatact tggtgtagta tttgtgcatc aaaaaatcct gccatgtatc aaaccctgag
    33481 caactatcct caagtgttac atattgcatt tcatccaagc tcaccttatc cgggttgtgt
    33541 tttcaatgtg caaaatatgg cagagaatga tgcccgtgca aattattata atgcatacgg
    33601 tggcacacca aaaatcgttt tgaatggaaa attattgtcg agtaacactc ctctcataaa
    33661 taatacaaca attgacacag ccttaaataa aaacagtcct gttgaaatat cggcaactga
    33721 attattgata ggacttgact caatgaaagc aagagtagtg gtgcgaacta ctggcattac
    33781 taacattact aacgtgaggc tatttgccgg tgttgccgaa aatcctgtaa actacacttc
    33841 gccaaatggt gagcaaattc atcacgatgt atttcgcaaa gcgttaactg ccgcagtagg
    33901 taatcaaatt gtattgccac aactgaatga tagtttggtg tttgaattta cttatttcat
    33961 caatcctatt tggaatgtga atgccattta tactatggct tatgttcaaa tagaagcatc
    34021 aaaagaagtt ttgaatgcag ttaaatcgat aagagttttg gcaaattcat taaatgaaaa
    34081 taacgaaaat gattttgcaa tccatttcaa ttcaattaca aatcaattgg tagttgccag
    34141 ttataatttc aaatcgaata cacattactc tattttgaat attaaagggc aagtgatagc
    34201 tgctggcgat gcaagcactt cggaaattac agtgaataat ttacctaagg gtgttttata
    34261 ttttcgtatt ggacgaaaag ttatgaaatt tgtgaatttg taaccgtatt tttttggtat
    34321 tttttggcta tcacttttgt gtttttgttt ttttaatgat tgtataaaaa tgcgtttcga
    34381 atttatttcg aagcgcattt tttattttaa acaagcgtct cagtatttga gggatttttg
    34441 attactgaaa atttcaacca tgcattaaga tattgtttgg atgcgcgcgc accattagaa
    34501 tatacaacta ccttgtttac aaatcacata attggaaagc acttaacgta tgtagaaagt
    34561 tttaaaagtc gattttgtat agaatttggg cgaaattcta tagaggatgt agaacaaaag
    34621 aaaatattat ttttcttcgc ccacagaatt ggtcgattgg ccattttaat ttttaattgc
    34681 gcattctaca ctattctgga tatacgcttt tgaaccaaag atgccttttg cggcaatata
    34741 tttgaagaga gtctaattct aactaaataa ataatatgca aaaacttaac ttaagattat
    34801 tggcaatgtg gtcgttgctg attgtatcat tcacttcgtg tatgcgcgaa aatgaagaac
    34861 ctgtcatttc taattctggt aatgatgtaa accaaacatc aagcgagaag aaaatgaaca
    34921 gcaatgaaat tccaaccgca tggatggatt taacattgaa acttattaaa actacacccg
    34981 gttactcacc acctgtggca tcgcgtacat tgggttactt aggtgttact ttgtatgaag
    35041 ccacagtaag tggctcggtt gtgaacaagt ctattgcgac acaactaaat atcaatccgg
    35101 tgccacctgc cccgcaacca aacaaatatt attattggaa tatttgcgca aatgcttcta
    35161 tggcacaggc tgcacgaaag ttttttatta ctactactgc agcaaataaa gtttcgatcg
    35221 attcacttga gaattatttt aatgcgcaag cacttgcaaa ttataataac gtagttgtaa
    35281 ataattctat cacctatggt cgcaatgttg ccgatgccat ctacaattgg tcggctaccg
    35341 atgttgttgg tcacgaagga tatttgaaaa atgttgatcc aaactatgtt ccgccaacag
    35401 gtcctggaaa atggattcca acaccaccac tttttgctcc agctgtacaa cctcattggg
    35461 gtgatgtacg tccattcgtt gcgcaaaatc caaacatttg gatgccaaac cctttgacat
    35521 attcattgag cccacagtcc aattattata aacaagcgct cgaagtttat aatacaaaaa
    35581 tcaacctgac agctgcacaa tcaacaattg ctcaatattg ggccgatggt ggcggaacca
    35641 taactcctcc gggacacagc atgaatattg ccactcaagt tattcgtaaa gaaggcaaca
    35701 atttgtacaa aactgccgaa gtatattcac gtgtaggaat ggctcttgcc gatggattta
    35761 ttcaatgctg gaaggaaaaa tatgtgcaca acgccatgcg ccctattact tacatcaatt
    35821 actatattga tccggcatgg acaccgttga ttgctactcc tccgtttcct gagtatagct
    35881 ccgggcatag ttcacaaagt ggtgcagcat cagaagtact tacagcaatc tatggtgcaa
    35941 actattcatt caccgattat tcaaaagtgc ctgatggatt tgcgccacgt tcattcaata
    36001 gcttttatgc ctttgcagat gaggctgctg tgtcgcgctt gtatggcggt attcacttcc
    36061 gtgctgcaaa cgaaaacggt gttatttgcg gaagaaaagt tggaagcaac attatgaaaa
    36121 ttgtttataa gaagtaatat atattaagtt taaaattgaa cctgcatcaa taaaaggtgc
    36181 aggttttttt atgctttttt tattgcagaa atatataatg tgtcggcacc atcatcactt
    36241 cccgaaatgt attttgaaaa tagaacagaa tatttttctt tatcgatttt gtttatcact
    36301 ccttcgtttt cgtctgcaaa aacagaacag gtaatataat acagcacccc attctttttt
    36361 aaacattgaa ggcaattgtt taaaatatca aattgctttt gcgaaaatac cggtagtagc
    36421 aaagaatcga attgcaataa attttcggga tttcgccccc atgtaccaga accactgcag
    36481 ggagcatcaa caataatttt gttgaacaac acaccatcgg gccattggtc ttcggcagca
    36541 ttcatgggtt ggtttttaaa ttttgtaaaa cctgttttca aaatccgctc ctttaggttt
    36601 tttaaaatgg gtatacggat atcagtacaa gcgatgctaa catttttata ctcttttgtt
    36661 tgcagtgtat caatcagtgc aagtgttttt cctccggcac cgcagcaggc atcccaccag
    36721 gtttcgttgt ctttcacttc aaataattgt gtagttgcaa aagacgattt atccattatt
    36781 tcgaagccac cttttgcaaa cgaatcgagg tcggttgcat ttgaagccgg ctgcagtttt
    36841 ataatgttgg tttcaaattc gaaaggaatt ttattgtttg taagatcttg ttttacaaaa
    36901 tctatgaatc cattatttac ccgtagccaa acgttgggtt gattgaagtg cgagaataag
    36961 aacgattcgt ttttgaaatt ctcgctcagg ttattgtagc agggaaacaa atcctttaaa
    37021 tcaatttcag ggtattgctt tttaagaagc tgtaattttt cattcaatgg tttgtttatg
    37081 ctattttgca aaggttgaac aagagaagca tcaaacacag attgtataaa ttcatcggtt
    37141 gcatcactac atagataata agagcaggct agtttttctg tgaaaagaaa atgtttaaac
    37201 atattgcctg tgcgaaaaaa agtgtagcat gcattgcgaa taattttccg gtcgcgcgag
    37261 ccggcatttt tatttttggt aaaataattt tttaaataat gttggaatgg agtttcgccc
    37321 gtatagcttt caataattga ttgtatgcgt tgtaattggc ttgtatatct actcatgttt
    37381 atattgtttt tattttgata caaaggcgct caacacattt tttttaatta agaaaagaat
    37441 tatcctttta tatatttcgt atttgcattt cttagtgcct tcgagccttt gtggcaaagt
    37501 ttttttagaa ttattgtttt gtcacgaaga cccaaagtcg gaaagtttta atctggtgac
    37561 tcctccaaaa agtccaaaaa agaaaaatgc caccaattca ccaaaacact aaaccccaca
    37621 aaacattata aatgatttgt ttacgattta gtgaaatttt gtgccttcgt gtttttgtgg
    37681 caaaaataac ttttcggaat ggactcaatc ttgaaatgag cactaatttt tcattcattg
    37741 aaaaaacaac ttcttgtttt gctaatagtt acaatttata tttcatttat tttctactcc
    37801 cgcacaccac ctgcaaccaa tctgcatcat tcaaatattt ttgtgctata ccaagtaaat
    37861 cattcgatgt gatgttaact aaaacattga ggtaattttc atagtaacta taaaccaaat
    37921 tattggtgag taaaactttg aaacgttcgg caagggcaaa aggcccatcc caactgcgca
    37981 ggaaaacacc actcaaataa tttcgcactg tatttaattc ttcttccgga attggttcgg
    38041 tgcaaagtct gtttatttcg aaataaattt cctttatagc tgcatcgcac acatcactgc
    38101 caacttctgt cgatatagta atagaacccg cttttggata agctacaagt cctgcaccta
    38161 tgccatatgt gtaacccttg tcctctcgta tgttcgacat tagtcgcgaa ccaaaataac
    38221 caccaagcac agttaccaaa atactcagtg caggaaaatc gggatgatgt cttgttattg
    38281 cccgcttacc aattcgtata gcagactgaa cagcattttc tttttcgata aaaacatttt
    38341 ccagtgcatt atcgtaagtg tatttcttat cctcattctt agctgcatta attttttgta
    38401 ccccgaatat gctattcaat tgttttacaa catcatcatt tattttgcca caagctatca
    38461 aactgtaatt tgtaccaaca taattttgag tatgaaaatc ggtcaatcct ttttgagtaa
    38521 gtgataaaaa atcttcctca tcatttgagt gctgataggg aaatccgctg aacatttttt
    38581 tactgaaagt ttttcttgca aggtactcta ctttttgctg ctgcactttt agcgattgca
    38641 aactccgttg aaaataagaa tccaattccg tttgtgggta tgtggcatca taaattattt
    38701 cgctcatcaa aggcaataaa tttggcaggt gcctggtaag ggttgtcaaa caaaaagaag
    38761 cattgtgaaa ttcaatattt gtttcgagcg atgcaccata aaactctacc gtttccgcaa
    38821 tatcgtaagc agtctttgtt gatgtaccat catctgcaag ttcgcaggct acttgcgcca
    38881 atataggatt cgtgggttta tccatgccgg catttataag caattctatt ttgcatactt
    38941 cgttggtgcc ggcatctatc acatacattg gcataccatt atcgagcatt attttatttg
    39001 ccttggtaag tttaaaatta tctaccgcgt taagtgccgg ttgaaacgtt ctgtcaattg
    39061 atgccattgt taatttgttt ttgttatcgg ttggtaacac aaaatattgg tattgtcttt
    39121 tgcaaatatc tgttgcgcaa tgtttgccaa atcctgtttg gtaatgtcat tatatttttc
    39181 ggcttgctca tttactgcag caacatcgcc aagcatatcg tagtaacaca aattcaatgc
    39241 tttgttggcc acacccaatt cgttaaacac gagcgatgct tctacttgtt gctttacctt
    39301 ttgtaattcg cggtcgtcaa tctttccgtc cataatttcc tgcaacactt cgtccactgc
    39361 tttttcggct tcggcaaatg tttttccatc atttagccgt ccttcaataa tgaacaaact
    39421 tttatcaagt tcgccaagca tgtaagccga aatggtggca aatatttttt tctgtttcac
    39481 taattgaaaa taaaggcgcg aagatttgcc tcgcgacaaa atatcggaca ataaatcggt
    39541 ggcataaaat tctttatcat atcgtgagca acaatgaaag gcaaaataaa tggcacttgc
    39601 cggcacatcg cgttccagaa tttgtctgcg caattctgtt tgctttggtt cctgcggcaa
    39661 aggcgaaatt tctaatgctt gctgctttat attgccaaac caattttcga ttaatgtttt
    39721 cgtgtagccg aaatcgatat cgcccgctac acttaatata gcattggcag gatgataata
    39781 ttttgcgaaa aaagatttta catcttcaag cgttgcatcc tctatatgcg aaatttcttt
    39841 gcctatggtt gcccatttgt atggatgtgt ggtatagcat agtggtctga gataaagcca
    39901 tgcatcgcca tagggctggt tgaggtagcg ttgcttaaat tcttcaatca caacgtggcg
    39961 ttgtacatcg aggctctttt cgctaaaggc tagttgcatc atgcggtcgc tttcgagcca
    40021 gcaggctgtt tcgaaatttt gcttgggcag ggttagataa tagtttgtaa aatcattaga
    40081 agtaaaagca ttgttttcgc caccggcacg ttgcaaaggt tcatcgtata caggtatgtt
    40141 tactgagcca ccaaacatga ggtgctcgaa taaatgggca aagccggttt ttgtttccac
    40201 ttcgtttcgt gcgcccactt tgtacagaat attcatggca actataggag tagatgcatc
    40261 gtggtgcaat attaccgtta gtccattggg caatacgtat cgttcgtaac ttatcatttg
    40321 attatttatt tggaggcgca aaactattta aaaactatag aacgtcttaa taaagttatg
    40381 aaatgtttat tgtaaatatt gcagtttctt gttcgggcaa atgggcataa tactctacaa
    40441 ataatttgtc ttttgtttct caaaggtttt tgttgaaaca taaatgttag aaaagcgtat
    40501 ttgagctggt aaatatttac taaaacaaaa acttaacatt ggaatttaag ttttgttcta
    40561 tatcttgcac tatcaaaaaa aatcaacaaa atgatcaaaa aattcctttc aattgtatcg
    40621 atgttatttc tgctagcaaa tattgccaat gcaggaacta caaaatggat taacatcaac
    40681 agtgcaaagc ctgccgaagc tacatcaaaa cttatattgg ataatggttc acaaactgtt
    40741 gtcgaattta atttgcccgg tttcgatatg aaagcggtga gtgctaataa tcttgatgcc
    40801 gctagcatta ttcctaaagc agccggcacc acacctttac tgatagacgg tgcgcccgat
    40861 ttgagtaaat atgccttttc ggttatcatt cctgataatg ccaaaatgaa ggtagatatt
    40921 gaaaactctt catacattga ttacaaggat attaagattg caccttcaaa gggtaactta
    40981 tatcgcaaca ccgatccatc taccatccct tacgtatatg gtgatagtta taagaccaat
    41041 gctttttttc ccggcaagtt ggctgagttg caaagccctt atatccttcg cgatttccgt
    41101 gcgcaaactg tttggctgta tccttttcaa tacaatccgg ttactaaagt gttgcgtgtg
    41161 tattacaaac ttaaagtaca aatagttaat gatggtatca gcaatataaa tgtattgcaa
    41221 aggaaaggca ctgcaaaaaa aatagataaa gaaatgtgcg ccatctataa ttcgatgttt
    41281 aaaaatggag gcaacaacac aattaattac gcacaggtag acgaagatgg aagtatttta
    41341 atattggcct ataaccagtt tatgagcact ttgcaacctt tcgtagattg gaaaattcgt
    41401 gaaggcattc attgcgaaat gatagatgcc gctagtgccg gtgcaacagc tgttgccatt
    41461 aaaacataca tagcaaatta ttacaacatg catcccgaat taaaatatat tttgattgcc
    41521 ggtgatgccc aacaagtgcc aacacctatt ttatcgggtg gtgcttctga cccgagttac
    41581 ggatatttaa gcggcagcga ttcgtatgcc gaagtttttg taggtcgttt ttcggcacag
    41641 aatattaccg acctgcaggt gcaggtgcaa aaagtaattg actacgaaat aaaccccaac
    41701 cttagtttgc agcattttaa taaaggggta gtggtagctt cggatcaagg cccgggcgat
    41761 gatggcgaaa tggactggga acacgagcgc attattcgcg acaaagaact tacgtataca
    41821 tacatggata taaaagaatt atatgatggt acacatccgg gtggaaatga tgatccgggc
    41881 gatccttcga gtgctgattt gtttaataaa tttcaatcgg gtattaatgt tatgacttac
    41941 acagggcatg gcagcaataa ttcgtgcagc acaaccggcc ttagcagcaa cgatgttgat
    42001 aatatgacca actacgaaat gcttccattt atatggagtg tggcatgtgt aaatggaaat
    42061 ttcaatcaag gcgattgctt tgccgaagct tttttgcggg cgcaaagcaa tggccaacct
    42121 acgggggcga ttgcaacttt tatgtcatct ataaaccaat cgtggaatcc gcctatggac
    42181 ggacaagatg aaatggttga cttgcttgta gaatcatatg cagcgaatat aaaaagaacg
    42241 tttggtggtt tatcaattaa cggatgtttc catatgaacg atgcgtatgg tgcagcaggt
    42301 gatgaaatga ccgatacatg gcatgtgttt ggcgacccat cacttatggt acgcacagcg
    42361 cctccaactg taattagcgc aacgcacgat gcctacattt cattgaatca atcaactttt
    42421 actttgcaat gcaacaaaca aaatggtgcg cttgcatgcc tgtcgaacaa tggtgaaata
    42481 cttgatgtgc aacctatagt aaacggtaca gcaacattta actttaatgc acttattgtt
    42541 ttagatacca ttcaacttac tatcacaggg ttcaattgca aaccatatct gaccgactta
    42601 cctgtattgt taaccggtct tgatgagcag gaaaataatt tatcactttc ggtttttcca
    42661 aatccggcaa atgatatgtt aactgtaacc attaaagatt tcgtgtcgcg aactaataat
    42721 gtagaaatac tttccgaaac cggtgcacag gtgatgaatc tgacttttgc agaaaatagc
    42781 agcagtattg aagttcctac aaagaaatta tcgaatggtg tatattttat ccgttaccaa
    42841 aatggaaacg atatttgcaa aaaacaattt gtagtaaagc attaagattc aataaaaatt
    42901 aaaaaaaaag actgccagtg agcagtcttt ttttttggga aacaattgcg catagcttca
    42961 attactattc ctttgcaacc ttatgcaagt acaggcaaca tataaaaata tctggtcgct
    43021 ggcttttcct attataacgg gagcactggc acaaacggtt ttgggcgtta ccgatactgc
    43081 ttttttgggt agagtaggtg agacggaact tgctgcatcg gcaattggtg gtgtatttta
    43141 ttttacgctc atcatgatag gcatggcagt atcgataggc gcacaaatta ttatagcacg
    43201 caaagctggc gaaaatgacc tcaatggaat aagccatgtt tttaagcaat cgtttttggt
    43261 gttgggtggt gttgggttgc tcatgttttt tattgctcat tttttagcac ctgttttatt
    43321 tccatatata attcacgata ctaaggtaac cgaggccgcc acaagctatg ttatctgctg
    43381 ttcatatggt ttgttgcttt ggataccatt gcttagtgtt cgcgcatttt tggtaggaat
    43441 cagcaataca aaactcatca gcatacaggc aataattatg tgtgccatca atatagtgct
    43501 gggttacatt tttatttttg ggcattatgg ctttcccgca atgggaataa gtggtgcggc
    43561 atatgcttct gcaatttccg aggtgcttgc atcgttatac gcactatcca ttatccgttt
    43621 tcacaaaaat tataagtcgt atctgttgtt cgataatata caggctaaca aagcaaccat
    43681 caatactatt ttgcgattga gtgcacctat tgtattgcaa aattttttga cgatgggagc
    43741 atggtttgtt ttttttgtga tgatagaaaa attaagcacg catgcacttg ctatttcgaa
    43801 tgtgatacgt gctacttaca tgataatgat gacacctgca tggggatttt cgagtgcggc
    43861 caatagcatg acaagcaacc tgatagggca gggcaaagcc gatgaaatat ttttgttgct
    43921 gagaaaaatt atcaccatga gcaccaccat cattaccgct tttatttttg taatggcctt
    43981 gtttcctcac tatatattaa atgtaattac caacgaccag caacttatag aagactgtat
    44041 acccacatac tatattattt gtgtggccac tgtggtgctg agtataggct taaatttttt
    44101 gtcggcagta tcgggtacgg gtgcaacccg cacagcaatg tatattgaaa tgacaaatat
    44161 tttttgttat cttgtattcg tttatgcaac atgttttatt tttaaaacac gagttgaaat
    44221 tgtatggctc agcgaaatat tgtattggac aatgatgggc gtattatctt tgctttattt
    44281 aaaaaccaat aaatggaaaa atataaaaat ttgatttttg tttttgcctt tgcctttgca
    44341 ggtggctggc aatgtggcga tacaaaagaa gcgacaaaat cttcagtgcc agcggaaaca
    44401 aagatagcac cgaatataga aaccgttgca gccggttata tgaatgcaaa agtggtagac
    44461 ctcacagggc ttgatgggtg taaatttgta ctcgaacttg aaggtggcaa acaacttgtg
    44521 cctaccaacc tgaaacctga gttttgccgt gccgacatcg aagtttcggt aaaatatttt
    44581 gtgccaaaag gagcagtcaa tacttgcatg gcaggtacca tcatagaaat tactgacatt
    44641 aaaattcgtg aacgtagaaa atgaaaattc aaaggcctga acagtatttc gaacaatttt
    44701 caggagagca aaaaattatc ctttatgtgt tgcacgattt tattttacag catgaaggag
    44761 ttttttgccg cattgcttac aacattccta tgtattatcg caatggatgg atgtgctatt
    44821 tgaactttaa aaaaaatggt gatattgaat ttgcatttac aaatggtgtg cacttgccaa
    44881 acaaaaacgg attgttgcaa accaatgacc gcaagcaagt tgcaggcact tttattaacg
    44941 atgccatgaa aataaatctt gatgccttag aagaaacaat gcaagaggct attgcattcg
    45001 ataatattaa atacaaacca cgaaaaagaa cataacctat gcgcattatt ttttatggca
    45061 ctcccgattt tgctgtgcca tcgttacaaa ttttaataga aaataaattt aatgtagttg
    45121 cggtagttac cgctgccgac tcgcctgcag gccgtggttt gcaaatgaaa ttttctccgg
    45181 taaaagaata tgcaatttcg caaaacataa aagtgcttca accgcataaa ttaaaagacc
    45241 ctgttttttt ggaagaaata aaatcattaa tgcccgacct tcagatagta gtagcctttc
    45301 gcatgatgcc tcaagtgttg tggagtatgc caccactggg ctcatttaat ctgcatggtt
    45361 cactgttgcc gcaataccgt ggtgctgccc ccatcaattg ggcaataata aatggcgaaa
    45421 aagaaacagg tgttaccaca ttttttttac agcaggaaat agataccggc aatattatat
    45481 ttcaagaaaa aatacccata gatgaaagag ataatgccac cacgttgcac aataagatga
    45541 tgcacacagg ggcagcactt gttttaaaaa ccgcttgggc cataaacaat ggagaagtgg
    45601 atgccacccc gcaaagtaaa gttgacacca atgaactaaa ggcagcacca aaaatattta
    45661 aagaaacgtg tgcaattaac tttaacaacg aagtggataa agtaaataat tttgtgcgcg
    45721 gattgtcgcg ctacccggca gcgtatacat acatagttag taaagataac gaaaggttgc
    45781 aacttaaaat atactccgct caaaaggaat atggtgaaat gaatgttgca gctggtaaca
    45841 tgcaaaccga tggaaaaaca tttcttaaat ttgcctgcaa caatggatgg ctgcatgcgc
    45901 tcgaattgca gttgcaggga aaaaaggcca tgactgtgca ggaatttttg cgaggcttta
    45961 aatttcaaaa caccaatgcg taaacatatt gtagtattta ctggagcagg aataagtgcc
    46021 gaaagcggaa tcaaaacttt tcgcgatagt ggcggactgt gggaagagca tcgtatagaa
    46081 gatgtggcca cacccgaggc atggcagcgc aatcaggcat tggtgctcga cttttataat
    46141 cagcgaagaa agcagttgct cgattgccaa cccaacgcag cacactatgc cattgcaaaa
    46201 ctcgaaacta cgtataaagt aacagttatt acccaaaatg ttgacgacct gcacgaaaga
    46261 gccggttcca caaaaattat acacctgcat ggcgaattgc gaaaagcccg gagcactgcc
    46321 aacccacggt tgttatacga tattgacgga tgggaattaa agcaaggtga tacctgtgaa
    46381 cttggatctc aactgcgccc gcacattgtt tggtttggcg agcttgttcc catgatagat
    46441 acagctgcat atattgcaga acaggccgat atttttataa ttattggaag ttcactcgag
    46501 gtgtatcccg cagcaagcct tatcaatcat gccccccaca gcagcataaa atatttggta
    46561 gaccccaatg ccaaaccttt gccacacatc gaaaatctca ccattaaaaa aggtaaggct
    46621 gcccacgagg taccggcctt ggtcgaaaaa ctattaattg cataatggaa aagctattca
    46681 cttccaccga cccgctatac caatggtttc ttatgccatt attcattttt gtatgccgcg
    46741 catccgatgt ttcgttggga acactgcgca atattttact aagcaaaaac cttaaaacca
    46801 ttgtgccttt tataggtttt gtagaggtaa tgatatggtt ggtggccgta caatcggtgt
    46861 tgagtcacgt gagtaatatt gccggttacc ttgcatgggg ttttggctat gctgccggta
    46921 tttatttagg tatacgtatc gataaacgat tagggttggg catgcaaata ttccacatca
    46981 ttacccacca caacatcacc aatttattag aagcagcagc acaacatgga tttgcaccaa
    47041 ccactgtaga tgccacaggg tctcgcgggc cggtaaaaat agtttacctc attatagacc
    47101 gtaccgaaaa cgctaatgtt aaagcactca tccaaactca ttgccccgag gcattctata
    47161 ccatcgaaga tgtgaaggat gctaatcagg gcgtgtttcc aaaccaacaa gaaaataaac
    47221 tcttgttcta tttaaagcgc cttgttccgc ttttcaaaaa ataatggaat tgccaccaaa
    47281 ggtaaggaag aatggattcc agcaatgaca ttaaagacaa caaaaaagta atgtgtgttc
    47341 tttggtgttt tctgtggcaa cacacattcg gcattcattc gtcttattcg tggcttaaat
    47401 gcatttgcat tttattcgga tttcattcgc gcattcgtgg caaaaatatt tcggctaaag
    47461 ccaataaaaa ttattaatac cgttcgataa atcgaacggc aatgaattgc tttcgcaaag
    47521 actttgtgat ttggtgcctt ggtggcaata ttattattca gaattcctgc gagaattgtt
    47581 ggccaccaaa tatttgcacc ttattcagca ttcattcgcg catatgcgtc agggaattcc
    47641 taaaaacctc ttttgtgtaa taagtttcaa acaaaatttt aattaaaatc tttttagcac
    47701 aaattttgtt taaatgtgtt ccatataatt agtagataat aaaaataact atatttggtg
    47761 cactattaga aagagaaagc aatgagcgag aaaaagacag gcaataacaa aacacacaca
    47821 acaggcaggt tgacgcatgt cgaaaaaatg catcctttca aaaccttcct gttttttgct
    47881 atggtaggca gcacacttat ttttctctca ctcgtttttc tttatgccat tcgcgtttcc
    47941 agttcaggcg tatcgcaaca ctttcaatta ccacgcacat ttacacttag caccattata
    48001 ttactcttta gcagctttgc actaagccgt tgcctgcgcg catttaaaaa cgatgaactc
    48061 cgcgatttgc aaatatccat gctctctaca tttatactgg ccattgcatt ttgcatcaca
    48121 caatttatag gatggaaaat gttggccgat gccggttacc tcgtaatttc gaaccccggt
    48181 gttaccttcc tttttattat ttcgggcttt catatgttgc atattgttgc agggttgggc
    48241 gcgtttgggt acctcaattt tcaaatattt catgcaacag gtaatgttgc aaaagccctt
    48301 ttatttctca gcgacaattc taaccgcacc aaactacaac tgtttaccat ctattggcac
    48361 tttgtcgatt ttgtgtggct ttgcgttttt gtgatgtttc ttttcacgct gtaaaaaaaa
    48421 tcgcatttta attcaaatta atatttgcga cagctgtaag tttctgcatt cttcttttca
    48481 atcttcgcaa tcaataatgg tattgatggt aatcattttc aacaactttg tgattgggtt
    48541 gtgtgcatca tatcgttatt acatccaatt gcatcgatga ctattgatac taaagtgttt
    48601 gtgtttcaat ataatcctga tttttaattt attgggctat aaccttttgc caatatccgt
    48661 ttcgatggca ttgagcccag aatagcaatt taaacttcgg tatatttact ttcgggtggt
    48721 ggggtattgg ttcttagaat gtgttattta ttgccgtgtg gcgacacacg gtaaagggag
    48781 aggccacggc ctcccgtaaa aaaagtatag ggcaaaaaaa aaccccgatt gctcggggtt
    48841 ttttgtatta gaaaaactcg tattattcct ttactatttt aacttttaaa acttcgctgc
    48901 ctgtatatat ctgtgcaacg tatacaccct ctgttaacga ttcgatgttc attgtagcag
    48961 tattggtacc ttcaatcaca ttgatgttta cgctgtgtac ttgtcggcct gtgatatcgg
    49021 taataacaat tgtgccttta ccttccacat cgcttctaaa tacaaatgca atgtcgcttg
    49081 ttgtaggatt tggatatgca ttgatgtcta gttcttctgc tacctttgga ttacgctcat
    49141 taagtggcat cattgcagtc cagtttgtgc tgctaacatt tacgcgcaac ttgtagcaac
    49201 ttgatacgct gaatgcaccg ttgtaaccaa acacacgcag tttgaatgaa ccggtggtag
    49261 ttgcattgcg aataacattt tcatcgcttg tgccggcaag tagcgatgat gcaagcaaca
    49321 catttccata gctatataac tccaagtcgt agtcgaatgg caattgcgat aaggttgctt
    49381 taattttaat atttggcgat acagtactaa atttatacca atcaacatct gtgcttgtac
    49441 ctatcatgga gtagatatct gtacttacat taataactgt aaaggtggca ttcgtgttat
    49501 taggttcgta cgaatttgca caattgccac ccggagctga ggttgtgaat gtgcttatgg
    49561 tactgaaagc tgaaagtact cctcccgcgc aaacagtgcg taccctatac tggtagttag
    49621 ttgaagcaac caaacctgca attggtttag tgttgagcgt tgacgttttg tttacccaaa
    49681 ctgtagctcc caccacgcga tattgtatct tgtatgaaag ggcacccagt gttgccgacc
    49741 aatttaatgt tgcagtggtt gatgtgatag atgtggccga taatgaatta ggaacgccac
    49801 aaccacccga agatgaagta gtataattgc ctatgctgct gtaagcactt gttgcaccat
    49861 tgcaaactgc ctgcacccta tattcataat ttgtggaggc aaccaggcct gtaagccctt
    49921 tgctgtttgt ggtcgatgta gtatttatcc atacacctgc gcctaccaca cggtattgta
    49981 cattgtatga agaagcaccc ggtacagctg cccaatttaa tgtaacagaa gttgatgtga
    50041 tggccgatgc cgataatgat gcaggtgtgc cgcaaccgcc atttggaggt gtgcacccta
    50101 atgatgtttt tagcgatgca cgcgctccat ttattgtagc atcaatgcgt agactttgac
    50161 ctgctgtaaa tgcattcata catgcatcat ccacatagtc catgtagttc ataaacatat
    50221 cgccatttgg gccattgccg catgtaacct ttggaaatgt tgggcaacca aaatttgaag
    50281 tttgttgagt tggtgtatca gctacttgat cgtttccgca atttgcatcg ccccaaatat
    50341 ggcttaaatt aagccagtgc cctacttcgt gggttgctgt gcggcctaag ttgtatggcg
    50401 tggaagtgcc gggtgccgat ggtccaccaa cgctaccggt tacgcaaaca actccatctg
    50461 tattggcagt gccgcccgga aactgcgcat agcctaacaa tccaccacct aagttacaaa
    50521 cccaaaaatt gaggtaagta cctgtaggcc aagcatcgga accacccgag gcagaaaatt
    50581 tcatgggatt gcccgcagat gaaaatgatg taaccgtagt agctttacgc acaataccag
    50641 tagtagcagc accattagga tcgcgctgtg ctaaacaaaa ctgaatattg gtagcagatg
    50701 ctattcccga aaatacgcca ggggtatttc cggcatcagc atttaggcgg gcaaagtctt
    50761 tgttgagcac atcgagctgc gcaaaaatgc gcgcatccga aatattttgt gccgcggtgt
    50821 tgtacaatat gtgaaatact accggtatag tagttatcac atttgatttt gcattttgat
    50881 tttgctgcac ataagtggca atatctcttt cttgtgcatc catacgttgc tttaacgtag
    50941 gatcgttttg caattgctct ttgagagcat ccatagtacc acaattgcgg tgactatgtt
    51001 gcgattgggc agctacattc ataaataatg ctactgcaga agcgaggacg atttttttta
    51061 acataatttt tgtttttaaa gatttaaatg gcgtcacaat ataaatgcat tctcaaataa
    51121 aaagcaaact atttttattt tgtttatgaa taaatttgtt aacatacttt taattgcagt
    51181 tttatttttt cattttgatt agtgaaaatt tgaagcaaaa cggtatgctt tgataccaat
    51241 accactaaac tgctaaacat tctttattag gaaatatatg cttttacatt ttggtgcaaa
    51301 catttgatta ggagtaacac atagcaacaa gcgtgacaaa aaaatcttta tgcggtgtta
    51361 ttgctcctgt tttttgctat ggtaggcagc acacttattt ttctctcact cgtttttctt
    51421 tatgccattc gcgtttccag ttcaggcgta tcgcaacact ttcaattacc acgcacattt
    51481 acacttagca ccattatatt actctttagc agctttgcac taagccgttg cctgcgcgca
    51541 tttaaaaacg atgaactccg cgatttgcaa atatccatgc tctctacatt tatactggcc
    51601 attgcatttt gcatcacaca atttatagga tggaaaatgt tggccgatgc cggttacctc
    51661 gtaatttcga accccggtgt tactttcctt tttattattt cgggctttca tatgttgcat
    51721 attgttgcag ggttgggtgc atttgggtac ctcaattttc aaatatttca tgcaacaggc
    51781 aatgttgcaa aagcactctt atttcttagc gacaattcta accgcaccaa acttcaactg
    51841 tttaccatct gttggcactt tgtcgatttt gtgtggcttt gcgtttttgt gatgctcctt
    51901 ttcacacttt aagaaatact attctgaaat tccatcagtt ttaaattttc tcaacaagta
    51961 atctttcgca aaccgtgtgg ctcaatgttt ctgaaggtcg ttttccatag ctcacaatca
    52021 tggttttgtc gaacggttct accgatttta ttttcagttt caaacccagg ctgagttcgc
    52081 gactatttaa aaatttcaga aactcgcccg aagtatttat tactgctgtt aactttatgg
    52141 tgtcgccact tttacattcg ctcagcttgt tgaattcttt ccaaaccact ttgccattct
    52201 tgtcgggaat aggggagcca tgaggatcca gttttggaaa gccaagcaac tcatccatct
    52261 tatcgaaaaa taaaggcgat tgtatatgct ctacctgttc ggcaatgggg tgaacatctt
    52321 cccagccaaa ccccattttt tgtaccaaaa acatttcggt aaggcgatgt ttgcgaataa
    52381 caatggccgc ttcttttttc cctttctcgg tgaggcgcaa gggtttgtag ctttcataaa
    52441 gtacaagttt tttttctgaa agttttttca tcatgctggt aacagtaggc atttttattt
    52501 ccagccgttt actcaactcg ttcacattca cctcgccttt gctattcgac agggtgaaca
    52561 gtgcttttaa ataattttct tctatcagtg aattcatagg gcaaatatat gaattgacat
    52621 cgcaaccatt caaacttata taaattattt tcaaaacaaa tttgttagat atgtctaaca
    52681 tattac
//
LOCUS       DAORQA010000007        51464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_113_length_51464_cov_34.765858, whole genome shotgun sequence.
ACCESSION   DAORQA010000007 DAORQA010000000
VERSION     DAORQA010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 51464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51464
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113_length_51464_cov_34.765858"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2991
                     /locus_tag="PK736_01365"
     CDS             <1..2991
                     /locus_tag="PK736_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRC32071.1"
                     /translation="PDGTKLYIYDKLNGFIVFNGSYTTWTQVTLSIPQLPSNVVPEKL
                     ALVPCNNNKDYNVICGTHPTLDNNAVQNLFHIYEWKNTYTNPVVKQDPLSFGTEFQYL
                     NHDAVDHIDEGVTAIEVDETNHKIYAATVRNTGTAIFSHIYCTSYNLYSGNISPVWTE
                     IIGNPNPFPNKTISCLKAYGRGACNEKLFICSRGLGTWVDDVGSATIFDQNDFSSNTP
                     ITGAGTLTTSVVVTGIVNINAASINIEAGYTITVNGGTLNILNGSHLFSCTDMWQGII
                     NNGGTVNIIESLIEDAEEALRMDAPNSIADIRYSTFNKNFVDIVLDGQGFLNGISFSN
                     SLFEDNTFKCNAALNTPHPQSFERTWANFMLTNATDVSINNNNNFADASYAVNGTNSV
                     AIVENNTFTNIANNNNSVQHEYSAINMREGNLTATSNNFNNCNMGIVTTGAVTSEISL
                     NTVTACEYGVSINGLYEGTSWIHHNTIEKTNNAIELWNISTQEPITVELNICNSAYDA
                     NNTPTSHCAIQFLQPFAYQTQVNIASNVITDYHFGVACIGAYGNAAMHNIVTDNTINY
                     QFEENEIGNDNYYGVYTEGCNYIAVHGNTFDWHDKAADPTAYASQVQGIRAQTTINSS
                     IDNNTMVQTGTGILMQDDCSNTFLSCNYFDNAAPGIFMDNVDLPTQGTANTPTYNTWV
                     GPYNGTLNNKFKIAGNSATGPIIFWYDGAANSTNQRWPRPSPPNLINPLAITALSLFY
                     CNGMPNIIVLDSTAQERLVDTTVASNSIVDSLREYYREAYSYASMSEDSSLLSNSLRL
                     AFYQQKQLENIGRFNEIMDMLRVGSIDSALLLTNNIIDNNAVEENLKQYYQILLPTLK
                     PTPILPNANDSVTMENIIYQNAYEGGEAVIKIRAYLRKIMVDHHAGFMRNSNTVMQKA
                     KQNIYVFPSPLTGSQHLNVVSKETAIATISIYDVTGKIMFQTIIDGFKKETEINCSAF
                     APGVYHIKVISTDTYQYNTTFIIF"
     gene            3043..4551
                     /locus_tag="PK736_01370"
     CDS             3043..4551
                     /locus_tag="PK736_01370"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32072.1"
                     /translation="MKVILFISVNFFMFSVFSQNRNNIWCFGDSAGINFANKSNPSTI
                     ISSAKSRGSCASICDTNSNILLYAYTRATVNGNTTLVKDKYHQLMQNGTQIVGEGWYY
                     ELIIIPKPSDTNKYYLFTIGAAGSSKLGLHYSIIDMTLNNGLGAVTQKNVQINNMKAN
                     DGLTAVQHGNGRDWWLIFRNWGGPANNVFYKYLIDVNGINLPDTQQIGTIINSGWQRF
                     KFNKKGDTLAVANYNGLIEFWTFDRCTGLLSNHTLIHPKINTSPPTEFWSCEFSPNGR
                     FLYIATNGYYNAAYLIQVDLLNPLLYAAADTIDSINYVIDPGGQLKRGPDDKIYWTCA
                     YYDGFNFNYPYPDTLYNTYNTYLSVINSPDSLGAACNFTPYSFYLGGARTYWGLPNNP
                     DYDLGPLLGSPCDTLQWTNLTPALSKGEGVMQITYIADWEKLFINASGLKGKNVTVTI
                     YDSRGSAIKNYDLKIINGYATVDVDCSGCSDGLYVVHLQSEKEVLSKKFVKN"
     gene            4754..6373
                     /locus_tag="PK736_01375"
     CDS             4754..6373
                     /locus_tag="PK736_01375"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRC32073.1"
                     /translation="MSSVKYIFVTGGVTSSLGKGIISASLAKLLQARGYKVAIQKLDP
                     YINIDPGTLNPYEHGECYVTNDGAETDLDLGHYERFLNTATSQANNVTTGRIYQTVIN
                     KEREGAYLGKTVQVIPHITDEIKRSIKLLGETGDYEIIITEIGGTVGDIESLPYIEAV
                     RQQIWEMAGNCCVIHLTLLPYLATTGELKTKPTQHSVKTLLEYGIQPDILVCRSEHPV
                     NQELKRKIALFCNVNINAVIESIDAPTIYDVPLLMMKEQLDKVVLNKLRLNMRHEPDM
                     EQWKAFLGKLKNPVNEVKIGLIGKYIELKDAYKSISESLIHAGVANECNVKVEWIHSE
                     KLDKDNIKDKLKNLHAVLVAPGFGNRGIEGKIDAIEFVRKNNIPFLGICLGMQCAVIE
                     FSRNVLKLENANSTEMDPDAKNPVIDIMASQKDITRKGGTMRLGEYPCVLLKGTKAAG
                     IYNKSKIAERHRHRFEFNNKYLKKFEEAGMIASGINQSPLGDLVEIIELKNHPFFVGV
                     QFHPEYKSTVENPHPLFVKFVKAAMERKAVV"
     gene            complement(6922..8001)
                     /locus_tag="PK736_01380"
     CDS             complement(6922..8001)
                     /locus_tag="PK736_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRC32074.1"
                     /translation="MVLLFCLLSLSADKAFSANPDNRKITEIVFCIDMSGSTSGVFES
                     FKKAYWKMISSVQGQMSTQHICVGLVGYSRESFKQDSNFVKVFVHPTENYEEVFLEFC
                     KIKTYMPGGSQHLGALVDAAVNRVTWSKRKGAQRVIIVIGNGDVNIGPVKLKAAIRSA
                     KRKGFVIQPVFLKSNIYKKEIKDWESFAAMSGESLYFVNMLDSTVIDNKKVDDLRVAN
                     SDFNQKIIYYGKTGRKTFDEINNSDLQTPADGLYERLKYKMFELKNENAHDWELVEYY
                     NTSEFELINIARETLPVQYTTVVADSMIQILKLSTTSKKTDYSNFQTEIEKIKPPPID
                     FAPADDKSMRFDAALQLIFRNAVNR"
     gene            complement(8187..9950)
                     /locus_tag="PK736_01385"
     CDS             complement(8187..9950)
                     /locus_tag="PK736_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC32075.1"
                     /translation="MGNKEEKKLDGLLNVFRYVKPYRQNFIGTIFLSVLLAIVSPLRP
                     WLTQLTIDNYIAKGDEHHLLLFTALMLITALLNSFLQIFYASNTNLLGQNIIADLRKN
                     LFSKLQSFNLTFFDTTPIGITITRLVSDMETIADIFTDGLLVIISDLLQLVAIIAFMF
                     YIDWRLALISLSTVPVLIIATRVFQKNIKKSFNDVRSAVSNLNTFVQEHLTGMRIVQV
                     FNREDEEYKKFQSINREHRTANIKSVWYYSIFFPVVELLSATAIGLIVWYGTRAILHH
                     ELQFGVVVAFIMYINLLFRPIRELADKFNTLQMGMVSSERIFKLLNLTKPIVKNGTLS
                     ADKLKGNIEFKNVWFKYNADDENSKWVLRNISFKVGVGEVVAFVGPTGAGKTSIIGLI
                     NRFYEIQKGEILIDGINIQEFDIHQLRTKIGLVQQDVFLFSDTIKNNITLYNEDISLN
                     DVKLAATEVGANRFIERLPGTYDFNVQERGTQLSVGQRQLLAFVRAGVYKPLIFILDE
                     ATSSLDSESEDLISKATENVSSKCTSIIIAHRLSTIRRADNIIVIDKGEIIQQGKHEQ
                     LVAQEGMYKRLYEIQLLNRNN"
     gene            complement(9950..10750)
                     /gene="truA"
                     /locus_tag="PK736_01390"
     CDS             complement(9950..10750)
                     /gene="truA"
                     /locus_tag="PK736_01390"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRC32076.1"
                     /translation="MRYFLELAYNGTKYSGWQIQHNAISVQQLVDEAIGKVVGKPTVS
                     LGCGRTDTGVHATQFYIHFDVENKISDTAKFIFQTNAILPFDICVFNLFDVDDKCHAR
                     FDATSRTYHYIIHHAKNPFVNNMSAYMPSKIDLGLMNECCQILYRYTNYECFCKSNTQ
                     VKTFECKIEKAEWYVCGEFTIFTITADRFLRGMVRAIVGTMMNVNNRKITLNNLEEII
                     QSNNRRNAGKSVNPHGLYLTQVKYNFISNPVLRLPVIIGQTENNSNIK"
     gene            11185..12171
                     /locus_tag="PK736_01395"
     CDS             11185..12171
                     /locus_tag="PK736_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC32077.1"
                     /translation="MMDIKEINEKIQQESAFVDLLNLEIGKSIVGQKYMIERLLIGLL
                     SNGHVLLEGVPGLAKTLAISSLSKAVDAKFSRIQFTPDLLPADLIGTMIYNQKKEEFT
                     VRKGPIFANFILADEINRAPAKVQSALLEAMQEKQVTIGENTFKLEEPFLVLATQNPI
                     EQEGTYPLPEAQLDRFMMKVVIGYPGKEDEKLIIRQNLSNEKTTINPVVNTESILKAR
                     SAVREVYMDEKIEKYIVDIVFATRFPKENKLQKLAQLINYGGSPRASINLALGAKAYA
                     FIKRRGYVIPEDVRAICHDVMRHRIGLTYEAEAENVTTEQVINEVLNMVEVP"
     gene            12579..13463
                     /locus_tag="PK736_01400"
     CDS             12579..13463
                     /locus_tag="PK736_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRC32078.1"
                     /translation="METQELLKKVRKIEIKTRGLSNQIFSGEYHSAFKGRGMAFSEVR
                     EYMAGDDIRSIDWNVTSRFNHPYVKVFEEERELTVMLLVDISGSGEFGTQQQFKREMI
                     TELCAVLSFSAIKNNDMVGVVFFSSKIEKFIPPKKGKSHILRIIRELIDLQPTEKGTD
                     IAMALRYFTNVIKKRSISFIISDFMSSNYSDAFKIAARKHDMIALKIFDEREQHIPNI
                     GIIQIVDPETGKTLTVDTSSVDLRNSYSQHFTNANKEFSNLTAKCGIDFVNLRTNQNY
                     IQPLMNLFKQREKGRRSV"
     gene            13460..14458
                     /locus_tag="PK736_01405"
     CDS             13460..14458
                     /locus_tag="PK736_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32079.1"
                     /translation="MMTFKRFLTPKYFSASVVAMFFLCLNFNLTAQPQQQVAATLDTN
                     VIRIGEQTMLRLSSAFNKNNKYNWFALPDTFNNIEIISRDTVDTIQVNNDWQLQQNIL
                     ITSFDSGNWVIPPAQLSFNLPGDTTTYFSSTSPLLIMVNGIAVDTSKAAYDIKSVIDV
                     PFSIMDYIWWIVGAILILAIIAGTIFFLRKSKIEQGAPQIVKIKKPAHEIALAALANL
                     KEEKLWQQGQVKEYHTNLTDIIRTYIGNRYNIHTLELTTDEIMQYQIIKSLPADVNLE
                     LKNLLQLADLVKFAKANPLANEHELSYSSAFDFVMKTKQSEQTPVIKTEQNNSTQK"
     gene            14476..15462
                     /locus_tag="PK736_01410"
     CDS             14476..15462
                     /locus_tag="PK736_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRC32080.1"
                     /translation="MQFAHPYYLWLLLLLPILLFWHLFFRKKNITSFNFSNTKPFEKT
                     NRSWRQYLLNVPIVLRMLTLAFIIVAIARPQSSSRAQNVTTEGISIVLALDISESMLA
                     EDFKPNRIEAAKKVAIDFIEGRPTDQIGLVVFKGESFSSCPITTDHGVLINLLRTINS
                     DMLPTPGTAIGEGLATAIARVKDAKTKSRVVILLTDGVNNSGSIAPETAGEIAKTFGV
                     RVYSVGVGTNGFAPTPMKTPFGVQYQNVPVQIDEDMLRKVSKETDGIYYRATNNKKLR
                     DIYAQIDKLEKTKVEVTEFRRYSEEYWPLAVVAGILFLLEFILRYLVLKKIP"
     gene            15575..16630
                     /locus_tag="PK736_01415"
     CDS             15575..16630
                     /locus_tag="PK736_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRC32081.1"
                     /translation="MEEFRWVNENLLYLYILLPLMVLCFYFMYRWKKNAINKFGQWTV
                     IAPLFAHVSKIKPVWKFVFMFLAATMLITAICRPQLGSKLEEVKRKGVDIVICLDVSN
                     SMDAEDLKPNRLERAKQAISQLVDRLQGDRIGLIVFAGESYTQLPITTDYGAAKLMLG
                     SIGTDMIPTQGTAIGSAIDLAASSFGDSIKKHNAAIVVITDGENHEDDAIEAAKEAYK
                     KGITVHTIGMGSVAGAPIPIKQYGQNTGYMQDESGQTVLTKLDENNLVQIADAGGGKF
                     VRATTSDDGLPLIMNEIDKMNKKEFSSKMFTSYEEQFQWALIIALFFLIIEFLISERK
                     SKWLEKLKLFEEEKPVV"
     gene            16651..17412
                     /locus_tag="PK736_01420"
     CDS             16651..17412
                     /locus_tag="PK736_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC32082.1"
                     /translation="MNTKTHICCKLIMMVLLLILQINLEAQTNATERKLVREGNKKYI
                     EKQFSDAETIYRKAMQTEHGSPTGTYNLGNALYRQKKYDEAVQQYQSTINSKANNESK
                     AKAYHNLGNTYLEQQKYQESVDAYKQALKLNSKDEQTRYNYAYAKSKLVQQQQQQQQK
                     KDDKQQQKKDDKQQEQKQDDKKEQDKNQQQQQQQDKEQEQKKKEEQQQQAQQKPKISK
                     EDAERMLQALKKDEKDLQKKMHKKEGTRSIISKNW"
     gene            17434..19269
                     /locus_tag="PK736_01425"
     CDS             17434..19269
                     /locus_tag="PK736_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRC32083.1"
                     /translation="MKINSLYKYFATTIILCALTVSIYAQQFNATINKTNAVVGEQLQ
                     ISFTFNGQGKNFGQPDFKNFQVIAGPSQSSNMTIINGNMTSSQSLTYWIVPQKEGTFK
                     IGPATITIDGKQIASNVITVSVGKGQQQQAQGNQRQQQQQNESGITSKNVFIKTSIDK
                     SSLYRGEAMVVTYKLYTNVSLVNLIPTEQPSFNGFWNNDIKLPAQPNFVRETYNGTAY
                     NAAVIYKAVLFPQQSGSLTIDPYKATVIARVQVKRNRNPNDPFDIFNDPFFGGGAQDV
                     EYKVTGENIKVNVKELPPNAPASFDGAVGRLNYDVKLDKLQTKANEPLALKVKISGRG
                     NLQLIDAPKINVPADIETYDPKVADNYSITEAGTNGSKTFEYLLIPRNEGEYIIDAIP
                     FSYFDLETRTYRTSNAGPFKIKVLKGEGGAASISNSTATIANKAEVAVLGHDVRFIKT
                     NMGEVLNINQLFYGSLKYYFLLLFPFALSIAAIFLKSRNTKLNSNITMVKSRGANKVA
                     TKRLALAGKLLTQNKNNEMYDEIYKAINTYVSDKFAIPISELTQDEMVSKLTLRKVNT
                     TTIESLTGVLSRCEMARFASGVAADAQVVFNDAMNVITKIENETV"
     gene            19256..20026
                     /locus_tag="PK736_01430"
     CDS             19256..20026
                     /locus_tag="PK736_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC32084.1"
                     /translation="MKLFNIKRVFIFFLLALWLGHEVMAAETKLFEQANTFYKNKNYN
                     KAIELYKKIIAKDNASAAVYFNLGNAYYKTSETGLAILNYERALKLQPNDDDIEYNLK
                     LANMQTTDKIEPVPQVFYQRWWTSLITAISLSKRTLVSIILAWLSCIFFIAYLFIRNM
                     QLKKIMFALALMLFAGLCVNAFLASQQYQQVNQKRFAIILATNPYIKSSPDEKSASLF
                     MLHEGTKVEVLDELIGWKKIRIANGNVGWIAADALEQI"
     gene            20185..20835
                     /gene="rpe"
                     /locus_tag="PK736_01435"
     CDS             20185..20835
                     /gene="rpe"
                     /locus_tag="PK736_01435"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRC32085.1"
                     /translation="MKLLAPSILSADFANLGKDVLMCNNSQCDWLHLDVMDGVFVPNI
                     SFGLPVIKAIKKHATKPLDAHLMIVNPDPFITHFKDAGADNLTVHYEACTHLHRTVDG
                     IKKAGMKAGVAINPHTPVHLLHDIIADIDLVCVMSVNPGFGGQKFIENTYSKIEALVQ
                     LIQQRNSKALIEIDGGVDMNNIKKLYTTGANVLVAGNAVFSTENPTQTIAAMKGIV"
     gene            complement(20960..21862)
                     /locus_tag="PK736_01440"
     CDS             complement(20960..21862)
                     /locus_tag="PK736_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32086.1"
                     /translation="MRIAHRYLTFERNDYLLEILQTNQIKINVTEKTYDEDLKSYSLE
                     FFLYEDEPNFAFKQNLVQHFTLLHQVTTEFSPEDFDKAEWFWFTVAPIGYPQPEGKFF
                     ETTYAIDNVCFKCEIGKRQKNPFRFKTEPKPGKSQFLGLHWVYDEMFVRDVAANIFRH
                     ANVTGMRFSNPVVSKSHTPIESIQQLHVDTVLSTGLVPDGLNVEICNKPHKEEKKDNA
                     ESSGEVDSPKNYCGQLKYNFPQRGALTFDKNIFEGQPDFVKPYEWFGSGGSASRPILV
                     SKKVRNIILKEKFRGAFFAPVFLK"
     gene            complement(21939..22097)
                     /locus_tag="PK736_01445"
     CDS             complement(21939..22097)
                     /locus_tag="PK736_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32087.1"
                     /translation="MLFRYNQANDNTNAEMIVTYDKGVRIDGTKHPHSWSIMDSQDCL
                     DWILKQLN"
     gene            complement(22362..23693)
                     /locus_tag="PK736_01450"
     CDS             complement(22362..23693)
                     /locus_tag="PK736_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32088.1"
                     /translation="MKKLIIIIMLSHLFSIAKTQTVYAMGSGVNYEIHCLFADTSSNK
                     IYASGTFTQMDGKNINYIAVWDGNQWDSIGITFGIGIVNSISSYKGEIYAGAAGYLDT
                     SGQWIYDCLTKWDGTKWVSACKGGSCKTLLNDFVENMYVSNNELYVTGRFDTIGGVQA
                     DMIAKFDGVNWFAFPPLDSFSLQNGSIIKSCIMYKGELYVAGGFTSPYNSKISKIARF
                     DGVNWNPVGNGFSNWAGINDLVVYKNELYAAGSFDKAHGDPGDNIAKWDGTNWSEVGT
                     KFIPGTQQIGEYNNKLYCREVINNYVYLCEWDGTNWTTGLGFNYWGDVHNFTTLNNDL
                     YISGFFSSVGGKVAHDVARLSWSTAIEESQLTNLLQILPSVSDGLFTINALTKSKTTV
                     SIYNIIDEQIFAFDLIGESKSLDMSSHPTGVYIVKAQTEKNIFSQKIIVAH"
     gene            complement(24072..24713)
                     /locus_tag="PK736_01455"
     CDS             complement(24072..24713)
                     /locus_tag="PK736_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRC32089.1"
                     /translation="MLIDKSKHAAEVFDKNALLYQDKFMNVELYHHAFDMFCDNIVKP
                     NAEVLDIACGPGNITKYLLYKRPDFKILGIDLSQNMIDLAKKNNPQANFRVMDCRAIN
                     SLPEKYDAIMCGFCFPYLSKPEAIKLIDDAGVLLQNGGVFFISTMEDDYEKSQYVKGS
                     TGDEIFMHYHQADYLCDALTKNNFEIIDMQHQPYPTNDATKVIDLLLLARKIK"
     gene            complement(24851..25324)
                     /locus_tag="PK736_01460"
     CDS             complement(24851..25324)
                     /locus_tag="PK736_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRC32090.1"
                     /translation="MKAKNILSLVLCVLFTLCVGAVSGVATASGINGWFMALNKPFFN
                     PPNYLFGPVWTALYILMGISFYMVLQAPASVVRKNAVAIFLVQLFLNFCWSFIFFKYQ
                     LLGWALVEIICIWLSIVTMIIIFSKINKTAAALQIPYLLWVTFASILNGAIWWLN"
     gene            complement(25335..25808)
                     /locus_tag="PK736_01465"
     CDS             complement(25335..25808)
                     /locus_tag="PK736_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRC32091.1"
                     /translation="MAFYQLEKTQKIPASIAMIWDFISSPVNLKEITPPHMGFVVTSN
                     TGAEKMYPGMIITYKVSPLLGIKLDWMTEITQVKEHEYFVDEQRIGPYTLWHHQHKIV
                     AIEGGVLMTDIVTYQPPLGFMGAIANTLFIKKQLQQIFDFRTIAVEKKFGKFITK"
     gene            complement(25819..26514)
                     /locus_tag="PK736_01470"
     CDS             complement(25819..26514)
                     /locus_tag="PK736_01470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC32092.1"
                     /translation="MSNYIVIGASSGIGKKVAQQLSTSGHQVYGTYCKNETHSEQNSL
                     QYNYLNVLDENLVMDFLPEHITGVVYCPGSINLRPFERIKPDDFVTDYKLQVIGAIKV
                     LQSVLPKLKKSENASIVLFSTVAVQSGLAFHSQVAASKGAIEGLTKALAAEYAPLIRV
                     NCIAPSLTDTPLAAALLNSEQKREANALRHPLKKIGTATDIANMAEFLLSEKAAWITG
                     QIFHVDGGMSTIK"
     gene            27275..>29027
                     /locus_tag="PK736_01475"
     CDS             27275..>29027
                     /locus_tag="PK736_01475"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HRC32093.1"
                     /translation="MKKQFLKLILTGALGLGSQLGFGQAELQVIHNSADPAAATVDVY
                     VNGALTLDDFAFRTATPFVTLPSGVQLNIGVAPGNSTSANDTLKNFQVTLMMGQRYVA
                     VANGVLNPGSFAPNPDGTSSAFTLFLQNNIKNMATNAGEVDFVAVHGSSDAPTVDVIA
                     RNVATLVDNASYSNITGYISVPAASYTLDVTPAAGSPIVASFTADLSTLGGGSAIVFA
                     SGFLDPSTNQNGPAFGLYAALANGTVVAFPSVSFARLQVIHNAADPAAASVDVYVNGN
                     LLLDDFGFRTATPFVDVPAGVLLNIGVAGGNSTSVNDTLKNFQVTLANGGQYLAVANG
                     VLNPANFAANPDGKSTAFTLFLQDMMRESAMMSTDVDFRVIHGASDAPTVDVIARNVA
                     TIVNDAPYSAITGYISVPAANYTLDVTPAAGSPIVATFKADLSSLAGGSAVVFASGFL
                     NPATNQNGPAFGLFAALANGTMVTLPQTSIARLQVVHNAADPAAASVDVYVNGNLLLD
                     NFAFRTATPYVDVPADALLNIGVAGGNSTSVNDTLKNFQVTLANGGEYLAVANGVLNP
                     ANFAANPDGKSTAFTLFL"
     assembly_gap    29028..29127
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <29128..29808
                     /locus_tag="PK736_01480"
     CDS             <29128..29808
                     /locus_tag="PK736_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32094.1"
                     /translation="ANFAANPDGKSTAFTLFLQDQMRESALVSTDVDLRVVHGASDAP
                     TVDVLAGASILVDNAAYGDITPYLSVPAANYTLDITPGNNNSAIVASFAATLSGIPGK
                     SAVVIASGFLTPSANQNGAAFGLLAVLADGTAFMLGNVTSIDENTGTINFGMYPNPAN
                     DMVMISLPDNETNAIVEITNSLGQVVLSQRPNNSLFDISLKNFVSGVYFVNVQTPAGK
                     STSKLIVQ"
     gene            30659..32329
                     /locus_tag="PK736_01485"
     CDS             30659..32329
                     /locus_tag="PK736_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32095.1"
                     /translation="MKPLSIANRKIFFNAICYFMIVAILNLTVSCSYYKVNKVRNLNE
                     DKSKVEVEKYRNLNKYFILHTGNEMWHVSIQNINAENKTMEVKLESVSAKHKLYLPNA
                     TNSTRIHEGRGGRAVLNEVHILTSQYVDTHDSIATINFSSITRIDIIEMDTWKNIGTF
                     VFTATTVAVAAYVISLIIIILTKSSCPFVYAHDGTGYNFKGEIYGGAIFKPLERDDYM
                     HLQNLSTDATNFKMKITNELLEVQYTNLVKAMLVEHNKNENILIDNRGEIHSIKSTQP
                     PLSALMNDTENMMTQLAAIDSNLCMFDGENNNHNVNDLVLKFAKPAKAQETKLVVRAK
                     NSLWLDYAFGEFTKLFGSYYNKFVEKQRTANHDSLLNWTVKQGLPLSVYLKQNGQWKL
                     IEHIPTVGPLASRDFCIPIDVSQINTPDMEIKISCGFMFWEVDYAGIDYSEDENIKTT
                     EMELQSAIDENNIDVKSLITENDKNYLIQPKPGNTVEFIYKLPAMKNEKAYDVFLQTK
                     GYYEHVRNYKGLPNKKYLTSMKEEGAFIDFTKSLFDKIKNDAGLTTSK"
     gene            32336..33571
                     /locus_tag="PK736_01490"
     CDS             32336..33571
                     /locus_tag="PK736_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRC32096.1"
                     /translation="MSTINKTDTLENKINAQSNNIISVTRRNATHIITGYKARLQRVK
                     FRLHILKLALQTYGWKRSTFSMLKQLEQKRRIVHGEFPITKWVQSNDRIAFTLYNPGY
                     PSQQCDDVLLEEMNRIAPSSVIARPIRFAHFAITKKCPLSCEHCFEWDNLNKKEILDY
                     NDLRNIVLKLKQAGISQLHISGGEPMLRVNDIVKLCEEFCNSMEFWILTAGYNLTKEN
                     ATRLKQAGVTGIIVSLDHYNKSMHNTFRQSKDAFDNAINAIKYARQADMVVAVTLCAT
                     KSFLTKENLYRYASFAHSLNVSYIQLLEPKAVGHYAGKDVTINTEQIKILEDFYFEIN
                     NSPTYTHYPIVVYLGYYQRNAGCLAAGNRALYIDTNGDVLACTFCHIPSGNALRDELM
                     DVVNRMRKKGCSDYIASVL"
     gene            complement(33666..35717)
                     /locus_tag="PK736_01495"
     CDS             complement(33666..35717)
                     /locus_tag="PK736_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRC32097.1"
                     /translation="MIFIIASVGVRAQFSQPLANMLQDTLNYYVSQITNVKGMSASVH
                     LPGQGTWQGTAGLSYSGAPITANMQFGIASNTKLFVAAAILKLTESNILSLNDSLKKW
                     IPTYANVNPNITIRQLLNHTSGVQDPIFLPPWVDTIMKNPTRVFTPQEVLSWVGAPLF
                     APGTNWSYSNVNYILAGMIAKSATGIHISKIIRDSILSPLNLSNSFYDVKENANGTLA
                     HRWYNSLDYNDTSRVGLNTAGGCAGAMFSTSGDMAKWYNALFTGQLLKPASLLELTTF
                     VTTPQAYTYSFGLEKQLWFGHTTYGHGGSTWGYKSRMVYEPCMGAVVCGLVNSWPAGM
                     DGITLLLYSVVLNHVPGCPPSITGPASVCKGQKSVIYSILPVANATSYIWTLPGGATG
                     TSTTNSITVDFASNAVSGNITVRGNSIYGVGQLSSLFVNVKALPAANITAAGPTTFCT
                     GGNVLLNAPLATNYLYQWKKGVNILPGDTMPSYNAKTGGSYKVIVTNKVTGCSKTTTT
                     GTVVTVNPLPTATITAQGPTTFCNGDSVVLAANSGIGYMYQWKKGSNILAGKTSINYT
                     AVSTGTYRVIVTNANLCSKLSSGKIVTVNCRTSSNADESAHFTFYPNPTTGNINIELD
                     KTYSNIQVTIKNIIGQEIFVKEYNATNIIDLNLDAWPGIYFIELKTDDEKLTVIKAVK
                     K"
     gene            36187..36981
                     /locus_tag="PK736_01500"
     CDS             36187..36981
                     /locus_tag="PK736_01500"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32098.1"
                     /translation="MTTKISSSNLLYSAIVITGIMFCTNLRSQNYCDPTFANGCSQWH
                     TNSMTLYNLNWFLGANSCMTSDYTADTATLTAGNTYPMLVSNGDWCGCGVWIDFNNDN
                     AFDSSENMYHMYIASQTAQHSFNLVIPANISTGAYRMRVIAGWGSDTYTVSPNGYGPC
                     GAYQYGNFNDFTVHVSNLAGINTNNTSQFFTAQFIAETNDLVVNMNDLVLEKAMLQLC
                     DVNGNIIETKAVTQNKEIMNLSTQPKGLYFLRFISGGKINTIKVLK"
     gene            complement(37175..37609)
                     /locus_tag="PK736_01505"
     CDS             complement(37175..37609)
                     /locus_tag="PK736_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HRC32099.1"
                     /translation="MKEITKKYTNGEVTIVWQPTVCIHSAICWRGENGLPEVFNPAEK
                     PWIKPEGANTKRIIDQIKKCPSGALSYFMNEDKENNAEVTAESIVEIAPNGPLLVYGN
                     ITVKDSEGNTTQKNKVTAFCRCGASSNKPYCDGTHRKINFEG"
     gene            38115..39734
                     /locus_tag="PK736_01510"
     CDS             38115..39734
                     /locus_tag="PK736_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32100.1"
                     /translation="MQKSFNNQVKKIREIVNRFDSDSNSLKISLIRDIQANKILFNHA
                     LKDYHECLLFIMSFPPDEKTRSLAEREVLRISGLLKKQPEKIKELFVNSGIPLSRYQA
                     AYSHDCTRWLLSHPDCTVAINEITNTSFDLNTALKVTIPAQLRNYTTAAYTNDVLLDE
                     LMPGKKLQLGYIINELSRLDNTPYVKDHYYEGLGIHTDIVPKSKLLSKAFNRIEIPET
                     FYHSEIIKRFDVVELLNRKLPDEAVLSSAEKEKLVLIIKNSMAITERETDPATYMKED
                     SLHLFHLDRGVSVALYGLPAERQLPMESYIGYTMFKNGYPAAYAGGWVFGWRSDFGLN
                     IYDQFRGGESAFMVAQILRVYRQLFNVNHFQVEASQFGLDSPEGIETGVFWFYYKLGF
                     RPNDLALRQLAKSEHIKISSRKGYRSSEKTLLKFTESNISMSLGKPVQPGVYDITSEV
                     KSLIIKKYRGNSVQAEKICVADFLTKTNCKDTFANAEFEVLKEVSLWAAAMNIADNDK
                     LNLLLQMVKLKPVDEYRYQELLRLFFGDNYK"
     gene            complement(39753..40403)
                     /locus_tag="PK736_01515"
     CDS             complement(39753..40403)
                     /locus_tag="PK736_01515"
                     /inference="COORDINATES: protein motif:HMM:NF039455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HRC32101.1"
                     /translation="MSTKTVYPRFIIVKDDFYDNPLQVHREAMEADYYEPRYYTGFRS
                     RKVYHQPGVKQKLEKILGIKINRWDTDPKDENGVFYFGYSKGNKKEIPGVHYDHPNDD
                     ITVVVYLTPGLPFDCGTSLWMHQKTHITDMPTAEDARRLKMKRADLLQLFETDSKKRN
                     KWQEIDRVGYRFNRMVAYPSGAMHSATSHYGESISDGRHYQTFRIGVDWSTFRMNL"
     gene            40766..41632
                     /locus_tag="PK736_01520"
     CDS             40766..41632
                     /locus_tag="PK736_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32102.1"
                     /translation="MKKVYLLFFLLHFTFKICEAQFILAGTVSGKDTYFDIVPDSALE
                     ALNVHLSPYPGETIKLDVDSDGIQDYQIDTYGGGGLGGGTGGCVIVPLGAHASVASHM
                     DTSAGCCPAQYIAQLVDTLVMGDTISNALSFYSGTNYLWSTTYGMAQAPIISEWNDIG
                     EHYIGIRLNYPQDTLYGWIRVEATNSGIFILTVKDMACNKNQHIGINEIYNSQKLIIA
                     PNPFSDQITVLCSDCSDALFVLYNFVSGKTLEKKITKSFLLNTEQLAKGIYLYEVRNI
                     NGVIKKGKAVKN"
     gene            complement(42690..43079)
                     /locus_tag="PK736_01525"
     CDS             complement(42690..43079)
                     /locus_tag="PK736_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32103.1"
                     /translation="MTKKLITKNILLGFLSWLIPYAISFLFFKPGGELMVPYATFKST
                     IMLVGVISGCYLLYRYFKFVDADFVTNAIIVGISWFAINIVLDILFLLPFTKTTYSDY
                     FTTIGIGYFAIPTISITLGYFLDRKPY"
     gene            complement(43133..45526)
                     /gene="ppsA"
                     /locus_tag="PK736_01530"
     CDS             complement(43133..45526)
                     /gene="ppsA"
                     /locus_tag="PK736_01530"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HRC32104.1"
                     /translation="MNSIKHFSEIGIKDIPLVGGKNASLGEMYVSLSKKGVAVPEGFA
                     VTSAWYWSFLKTNNLVPQLTQVLSLLDITTFDNLAEIGAKARKLILDSTFPVALKEEI
                     IAAYTSLNNSTGNNKSFAVRSSATAEDLPTASFAGQQETYLNVRGDENLIKACHRCYA
                     SLFTDRAIKYRTDNGFDHMKVALSIGVQTMIRSDIASSGVMFTIDPDSGFEDVVLISG
                     AWGLGENVVQGTINTDEFLVYKPFLHKLKQPIIAHKLGSKIKTMVYSGVSVNDIVKPE
                     EAIINIDTTEEKRNQFVLNDSEIIQLAKWACIIQEHYKCHMDIEWAKDGEDGKIFIVQ
                     ARPETVHSKKKQNVFKQYTLLDKGKKLCSGAGLGNKIVAGKARILSSPTEIAKLNKGE
                     ILVTERTDPDWDPVMKKATAIITDQGGRTSHAAIVARELGAIAVVGTGTGTKNIVDGQ
                     TITVSTAAGNTGTVYDGLLKWDEKEIDISSIQKTKTKAMLILAHPDEAFTFSQLPNDG
                     VGLMRMEFIISNTIGIHPMALKHFDTINDAEAKEKIEALTKQFKNKEEYFVQKLAEAT
                     AKMAVAFYPKEVIVRMSDFKSNEYANLLGGKQFEPGEENPMIGFRGASRYYHPLYKDA
                     FEMECKAMKIVRDDMGLTNIKLMIPFCRTIKEAQQVIEVMKGCGLERNINGLELYVMI
                     EIPSNALLAEEFAKHFDGFSIGSNDLTQLTLGADRDSELLSEIFSPFDAAVMQLIEMT
                     LTKAKKTGTHTGLCGQAPSDYPEYAQFLVKNGIDSISFNPDALIKGIENIAEAEKNI"
     gene            complement(45753..46322)
                     /locus_tag="PK736_01535"
     CDS             complement(45753..46322)
                     /locus_tag="PK736_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRC32105.1"
                     /translation="MEIVENKIFDKIDFTKQPIAKTEFEYCVFKNCDFANSELSEMVF
                     LECQFLDCNLSLAKISKTAFRDVKFSACKMLGLLFYNCNEFGLTASFDNCNLTHSSFY
                     KRKIKKTSFINCRLHEVDFTDCDLSESIFDHCDLANAKFENTILEKADFRTAYNYSIN
                     PELNKLKKAKFTLAAIAGLLDKYDIRIEK"
     gene            complement(46362..46910)
                     /locus_tag="PK736_01540"
     CDS             complement(46362..46910)
                     /locus_tag="PK736_01540"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRC32106.1"
                     /translation="MPTIFTHAFAAYTLSKTFFTKVTTQKLYTLVIICAMIPDADVVG
                     FQFGVSYNSMFGHRGFTHSIFFAAILAMVVKVIFFYSQSFTTSQGRIIFLSLFLATVS
                     HPLLDMLTNGGLGVALFAPFSNQRYFCPWFTPVDVSPIGAANFFTKWGLSVLISEIKF
                     IIFPCVIILLCAKLLNTKGGTK"
     gene            complement(47127..49673)
                     /locus_tag="PK736_01545"
     CDS             complement(47127..49673)
                     /locus_tag="PK736_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRC32107.1"
                     /translation="MEAKFSPRVKDVITYSREEALRLGHDYIGTEHLLLGLIREGEGM
                     AIRFLKSLGINLVDLRRSVENSVKGSSGNVSNLKNIPLTKQAEKALKITYLEAKIFKS
                     EVIGTEHLLLSILKDEDTIATQLLNQYGVTYDVVREELEVSRSNGFKSETPQQGEDDP
                     YARDEDGPGMTTPKKSGDSKSKTPVLDNFGRDLTKSAEEGKLDAIVGREKEIQRVSQI
                     LSRRKKNNPILIGEPGVGKSAIAEGLALRIVQRKVSRVLFNKRIVTLDLASLVAGTKY
                     RGQFEERMKAVMNELEKSPDVILFIDEIHTIIGAGGASGSLDASNMFKPALARGEIQC
                     IGATTLDEYRQYIEKDGALDRRFQKVIVDPTSAEETVEILTNIKDKYEEHHHVNYTPE
                     AIKACVTLTSRYITDRFLPDKAIDALDEAGSRVHLNNIHVPEEILDLENKIQEIKNEK
                     SKVVRSQRYEEAARLRDTEKQYIDELEKAKLKWEEDTRLQRFTVNEENVAEVVAMMSG
                     VPVHRIAQAETARLIQMPTELQGKVVGQDEAIKKVVKAIQRNRAGLKDPNRPIGSFIF
                     LGPTGVGKTELAKVLARYLFDSDDALIRIDMSEYMEKFAVSRLIGAPPGYVGYEEGGQ
                     LTEKVRRKPYAVILLDEIEKAHPDVFNLLLQVLDDGQLTDSLGRKVDFKNAIIIMTSN
                     IGSRQLKDFGMGVGFSTAAKLNSVDENTKSVIENALKKAFAPEFLNRVDDVVIFNQLD
                     KADIHKIIDIELATLYKRVEGLGYKLSLDAEAKDFIGDKGFDSNFGARPLKRAIQRYV
                     EDLLAEEIIKSELAEGDSIDLTFDKEQNQLKAVITKANNGNPKKPKKSAS"
     gene            49958..50320
                     /locus_tag="PK736_01550"
     CDS             49958..50320
                     /locus_tag="PK736_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRC32108.1"
                     /translation="MNKPTLVLGASENPERYSNKAIKRLVSAGHNVYAIGARTGKVDD
                     TFIQKDKQPLIGIHTVTIYLNEQNQKQYYDYVLQLNPERVIFNPGAENAEFEKMLEEK
                     NIETIEACTLVMLSIGAF"
     gene            complement(50340..51296)
                     /locus_tag="PK736_01555"
     CDS             complement(50340..51296)
                     /locus_tag="PK736_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRC32109.1"
                     /translation="MKNYLISCAFVVICANVQNSLAQDSILKCSPTAYIEKYKDAAIA
                     DMKKYGVPASITLAQGLFESDCGNSDLAKLANNHFGIKCHKEWKGETFIQDDETANEC
                     FRKYPHAQASFDDHSDFLRSRDRYKTLFDLDVTDYKGWAKGLKTAGYATNPNYAERLI
                     KLIETYQLYIYDGTTMIAGQPIAIDPITNNPITNNQSINNPITNNPITNQLIINHNNI
                     KATKVQRGDSWYKLAKQNQMELRDLLRYNDAQANDILKVDEIIYLAYKHRHADEEVHQ
                     VKKGDTLRSISQLYGVRLKRLMKFNKLPANPVLKEGDAIKLR"
BASE COUNT        16478 a         9655 c         9377 g        15854 t
ORIGIN      
        1 cccgatggaa ccaaacttta tatttatgat aaattaaatg ggtttattgt ttttaatggt
       61 tcatatacaa cctggacaca agttacactt tccattcctc aattgccatc caatgtagtg
      121 cctgaaaaac tagcattggt accttgcaac aataataaag actataatgt gatttgcggc
      181 actcacccca ccctggataa caatgcggta caaaatcttt tccatattta cgaatggaaa
      241 aacacttaca caaaccctgt tgtaaaacaa gacccacttt catttggcac cgaatttcaa
      301 tatttgaatc atgatgccgt agaccatatt gacgaaggtg taacagctat tgaagtagat
      361 gaaaccaacc ataaaatata tgctgctacg gtgcgcaata caggcacagc aatattttcg
      421 catatttact gcaccagtta taatttatat agtggcaata tttcccctgt ttggacagaa
      481 ataattggca acccaaatcc attcccaaac aaaaccattt cctgtttaaa agcctacgga
      541 cgtggagcct gcaacgaaaa attatttatt tgctcgcgtg ggttaggtac ctgggtggat
      601 gatgttggaa gtgcaaccat tttcgaccaa aacgatttct ctagtaatac tcctataacc
      661 ggtgcaggca cattaacaac tagcgttgtg gttactggta ttgtcaacat aaatgccgca
      721 agcattaata tagaagcagg gtatactatt accgttaatg gtggtacatt aaacatttta
      781 aacggctcgc atttatttag ctgtaccgat atgtggcaag gcattataaa caacgggggt
      841 acggttaata taatagaatc gttgattgaa gatgccgaag aagccctacg catggatgca
      901 ccaaactcca ttgccgatat tcgttattct actttcaata aaaactttgt cgacattgta
      961 ctcgatgggc aggggttttt aaatggcata tcattttcca atagcctctt tgaggacaat
     1021 acttttaaat gcaatgcagc tttaaataca ccgcatccac aatcgtttga acgaacttgg
     1081 gcaaatttta tgctcactaa tgctaccgat gtatcaatta ataacaacaa taattttgcc
     1141 gatgcaagct atgctgttaa tggcaccaac tccgttgcaa ttgttgaaaa caacacattt
     1201 acaaacatag ccaacaacaa taatagtgtg cagcatgagt acagtgccat aaatatgcgc
     1261 gaaggaaatt tgactgcaac tagcaacaat tttaataatt gcaatatggg catagttacc
     1321 accggtgctg tgacttctga aatatcgttg aacactgtta ctgcctgtga atatggagtt
     1381 tctataaatg gattgtatga aggtacttca tggatacacc ataacacaat agaaaaaaca
     1441 aacaacgcca tcgaactttg gaacataagc acacaagaac ccatcactgt agaattaaat
     1501 atttgtaaca gtgcttacga tgccaacaat acacccacaa gccattgcgc catacaattt
     1561 ttgcaacctt ttgcttacca aacccaggtt aacatcgcct ctaatgtaat taccgattac
     1621 cactttggtg tggcatgtat tggtgcttat ggcaatgcag ccatgcacaa tattgttacc
     1681 gataatacta tcaattacca atttgaagaa aatgaaatag gcaacgataa ttactatggc
     1741 gtatatacag agggctgtaa ttatattgct gtacatggca acacatttga ttggcacgac
     1801 aaagctgccg accccacagc ctacgcaagt caagtgcagg gcatacgtgc acaaaccacc
     1861 atcaatagca gtatagataa caacacaatg gtgcaaacag gcacaggcat acttatgcaa
     1921 gatgattgca gcaacacttt tcttagttgc aattattttg ataatgctgc accagggata
     1981 tttatggata atgttgattt gccaacacaa ggaacagcta atacaccaac atataatact
     2041 tgggtggggc cttacaatgg aacactaaat aataaattta aaattgctgg aaattcagcc
     2101 actggaccaa ttatattttg gtacgatggt gcagccaact ctacaaacca acgctggcca
     2161 aggccttcgc caccaaattt gataaatcca cttgctatta ccgctttgtc acttttttat
     2221 tgtaatggta tgcctaatat aattgtattg gactcaactg cacaggaacg tttggttgat
     2281 accactgttg cgtccaattc aatagtcgat agtttacgcg aatattatcg tgaagcctat
     2341 tcatatgctt ctatgagtga ggatagttcg cttctttcta attccttacg tttagccttc
     2401 tatcaacaaa agcaattaga aaacatagga agatttaatg aaataatgga tatgcttaga
     2461 gtaggctcaa ttgattctgc attattactt actaacaata tcatagacaa caatgcagta
     2521 gaagaaaatt taaagcaata ttaccaaata ttattgccaa cacttaaacc gacacccatt
     2581 ttgccaaatg caaatgatag tgtaactatg gaaaacataa tttatcaaaa tgcgtacgaa
     2641 ggtggtgagg ctgtaataaa aattagagct tatttaagga aaataatggt tgaccatcat
     2701 gcaggtttta tgcgcaactc taatacggta atgcaaaaag caaaacagaa tatttatgta
     2761 tttccatcac ctttgactgg ttcacaacac ttgaatgttg ttagtaaaga aactgccatt
     2821 gcaaccatat caatatatga tgttacaggc aaaattatgt ttcaaactat tattgatggt
     2881 ttcaaaaaag aaacagaaat taattgttca gcttttgcac caggtgttta tcatatcaag
     2941 gtaatttcga ctgataccta tcaatacaat accacattta ttatttttta acataaaaaa
     3001 acttcgcatc aacagtgcgc tgtttttttt tacgaaagtt caatgaaagt tattttattc
     3061 atttcggtta atttttttat gttttctgtt tttagtcaaa acagaaataa tatatggtgc
     3121 tttggagata gtgcgggtat taattttgca aataaaagca acccaagtac aataatttca
     3181 tctgcaaaat cacgcggaag ttgtgcctct atttgcgata ctaattctaa cattttattg
     3241 tatgcttata cgcgtgcaac cgttaatggg aacacaacat tagtgaaaga caaataccac
     3301 caactcatgc aaaatggaac acaaatagtt ggtgaaggat ggtattatga acttataatt
     3361 atcccaaaac ccagtgacac taataaatat tatttattta caatcggtgc agcgggtagt
     3421 tcaaaattgg ggttacatta ctcaatcatc gacatgactc tcaacaatgg cctcggtgca
     3481 gtaacccaaa aaaacgtaca aataaataac atgaaagcca acgatggcct tactgcggtg
     3541 caacatggta atggtagaga ttggtggttg atttttcgga attggggagg ccctgcaaat
     3601 aatgtttttt acaaatattt gattgatgta aatgggataa atttgccaga cactcagcaa
     3661 ataggaacta taatcaatag tggttggcag cgttttaaat ttaataagaa aggagataca
     3721 ttggctgttg ccaattacaa tgggctaatt gaattttgga cttttgatag gtgtactgga
     3781 ttactgtcaa atcatacatt gatacatcca aaaataaata cttcaccgcc aactgaattt
     3841 tggagttgcg aattttcacc gaatggcaga tttttataca ttgcgacaaa tggttattac
     3901 aatgcagcgt atttaataca agtggattta ctaaatccat tattgtatgc cgcagccgat
     3961 actattgata gtatcaatta tgtaatagac cctggtggtc aattaaaacg cggacccgat
     4021 gataaaattt attggacttg cgcctactat gatggtttca attttaatta cccttacccc
     4081 gatacattgt ataatactta caacacttac ctaagcgtaa taaactcacc tgatagcctt
     4141 ggtgctgcat gtaactttac accttatagt ttttaccttg gtggtgcgcg cacgtactgg
     4201 ggtttgccta ataatccgga ttatgatttg gggccgctgt tggggagtcc ttgtgatact
     4261 ttgcagtgga caaacctcac cccggccctc tccaaaggag agggagtaat gcaaattaca
     4321 tacattgcgg attgggaaaa gttgtttatt aatgcaagtg gattgaaagg gaagaatgta
     4381 acggtaacca tatacgattc gcgcggtagc gcaattaaaa attatgattt aaaaattata
     4441 aatggttatg caacagttga tgtagattgt agtggttgca gtgatggctt gtatgttgta
     4501 catttacaat cggagaagga agtgttgagt aagaagtttg tgaaaaatta gaaaatatta
     4561 ttagaatgaa atactataaa aatgtagttg tcaaaaatac aattccgacc acccttaaaa
     4621 agattaaatt ttaaagtaca tttttcaacc ttcattatct tatgccaagc gaaatttaaa
     4681 tttgccatat tgtaaaaagt tgcacataga tattacattt gcatcccgtg ttcaaaaaaa
     4741 acaaactaca ccaatgagct cggtaaaata tattttcgta acaggagggg taacatcctc
     4801 attaggcaaa ggcattattt cggcatcgct agcaaagctg ctacaggcaa ggggctacaa
     4861 ggtagccatt caaaaacttg atccatacat caacatcgac ccgggcactc tcaaccccta
     4921 cgaacatggc gaatgttatg taaccaacga tggtgccgaa accgacctcg accttggaca
     4981 ctacgagcga tttttaaata cggctacatc gcaagccaat aatgttacta ccggtcgcat
     5041 ttaccaaact gtaatcaaca aagagcgtga aggcgcatac ttaggcaaaa ccgttcaggt
     5101 gattcctcac attaccgatg aaataaaacg gtctataaaa ctgttgggcg aaacaggaga
     5161 ttacgaaata ataataacag aaattggtgg cacagttggt gatatagagt cgctgccata
     5221 catagaagcc gttcgacagc aaatatggga aatggctggt aactgttgtg ttatacacct
     5281 tactttgttg ccatatcttg ctactaccgg agagctgaaa acaaaaccta cccaacactc
     5341 tgtaaaaact ttactggaat atggtataca gcctgatata cttgtgtgcc gttcggagca
     5401 cccggtgaat caggaattaa aacgtaaaat agctttgttc tgcaatgtaa atatcaatgc
     5461 cgttatcgag tcgatagatg cacctactat ttacgatgtg ccactgttga tgatgaaaga
     5521 acaactcgac aaagtagtgt tgaacaaact aagattgaac atgcgccacg agcccgatat
     5581 ggaacagtgg aaagcatttc tgggcaagtt aaaaaatccg gtaaacgaag ttaaaattgg
     5641 gttgataggc aaatatattg aattgaaaga tgcatacaaa agtataagcg agtcgctcat
     5701 acatgctggc gtggctaatg aatgtaatgt aaaagtggag tggatacatt ccgaaaaact
     5761 ggataaggat aatataaaag ataaactaaa aaatcttcat gctgtattgg tggcacccgg
     5821 atttggaaat agaggaatag aaggaaaaat tgatgctata gaatttgtgc gcaaaaacaa
     5881 tattccgttt ttgggaattt gccttggtat gcaatgcgca gttattgaat ttagtcgtaa
     5941 tgtattgaaa ctggaaaatg ccaattcaac agaaatggat cccgatgcaa aaaatccggt
     6001 gattgatata atggcatcgc aaaaagatat tacgcgcaaa ggcggcacca tgcggcttgg
     6061 cgagtatcct tgtgtattat taaaaggaac aaaggctgcc ggcatttaca acaaatcgaa
     6121 aattgctgag cgacaccgtc accgctttga attcaacaat aagtatttga aaaaatttga
     6181 agaggccggt atgattgcat caggcataaa ccaatcgcca cttggcgacc ttgtcgaaat
     6241 tatcgaattg aaaaatcatc ccttttttgt aggggttcaa tttcatcccg aatataaaag
     6301 cactgtcgaa aatccacacc cactatttgt aaaatttgtg aaggccgcta tggagagaaa
     6361 ggctgtagtt tagtgtttag tattgagtaa gtagtattga gtagttggta ttgggtaggt
     6421 agtattgagt agttagtatt gagatgtttt gctatcggtt atttggttgt tgctttcgtt
     6481 attgtcatgc tgaggtacga agcatctctg cctaacgtta ggtcttggtt gttgctttcg
     6541 caattgttat gctgaggtac gaagcatctc tgtcaggcat tggtcattgc gcattgtcaa
     6601 gtgagtattc tcgaaattat aaactgttct gtaagaaatc gggggtcgtc cattttaatt
     6661 gtaaggatga gtgagtgata gaattattgg taaaataaag ttattcttac tgattgatga
     6721 tatagctaaa aagattatca tttgtcacaa tcatgatcga atagcattga ataaaattaa
     6781 cttgctttta atgacatcta aatatttata caaaaaagtt gattattatt tcggcaatgc
     6841 aattaaaaaa aactacacgt ctataaaaac ataaatcaat gaaaatcatt ttttgattca
     6901 atgcaataac ctaacaacca actacctatt cacagcattt ctaaaaatca actgcagggc
     6961 agcatcaaag cgcatggact tatcatcggc cggggcaaaa tcgatgggtg gaggttttat
     7021 tttttctatc tctgtttgaa aattggaata atcagttttt tttgatgtgg tactcaattt
     7081 caatatttgt atcatgctat cggccactac tgttgtgtat tgcaccggca aggtttcgcg
     7141 tgcaatattt atcaattcaa attccgaagt gttgtaatat tctaccaact cccaatcgtg
     7201 ggcattttca tttttaagtt caaacatttt atattttaat cgctcataca atccatctgc
     7261 cggtgtttgc aaatcggaat tatttatttc gtcaaaagtt ttacggcctg ttttaccata
     7321 gtaaattatt ttttgattga aatctgaatt ggccactcta aggtcatcca cttttttgtt
     7381 gtcgataaca gtgctatcca gcatgtttac aaaatacaaa ctctcgccac tcatggctgc
     7441 aaaactttcc caatctttaa tttccttttt ataaatgttg ctctttaaaa atactggttg
     7501 aatcacaaac cctttccgct tagcggacct aatggcagct ttcaatttta caggaccaat
     7561 atttacatca ccattgccaa taactattat tacacgttga gcccctttac gtttactcca
     7621 ggttacacgg tttactgctg cgtctaccag ggctcctaaa tgctgcgaac ctccgggcat
     7681 gtaagtttta attttacaga attctaaaaa tacttcttcg tagttttcgg ttgggtgtac
     7741 aaataccttt acaaaattcg agtcttgctt aaacgattcg cgcgagtaac ctaccaaacc
     7801 aacacaaata tgttgcgtac tcatttgccc ttgtacagaa gaaatcattt tccaataagc
     7861 ctttttgaaa gattcaaaca cacccgaagt gctgccactc atatctatac aaaatacaat
     7921 ctcggtaatc ttacggttat caggattggc actaaatgct ttatcagcac ttaatgataa
     7981 caggcaaaac agtaaaacca attttggtat tcttagcata ggataacgag gcaagaaaaa
     8041 aatagatgac cgatgttcaa cggcaaatat aaatactcga cttaatctac caaacagatt
     8101 cggcaaagaa attaaacttt ataaaaaagg ggctttcttc tatttaaagg tactgtgtga
     8161 tttgttccag aagttggtgg gcagaatcag ttgttccggt ttagtaattg tatttcgtac
     8221 aaacgcttgt acataccctc ttgggcaacc aattgttcgt gcttgccctg ttgtataatt
     8281 tctcctttgt caataacaat tatattatcg gcacgcctga tagttgacaa tcgatgggca
     8341 ataataatgg atgtgcattt gctcgataca ttttcggtgg ctttcgaaat aagatcttcg
     8401 ctttcactat cgagtgagga ggtggcttca tccaaaataa atatcaatgg cttgtacact
     8461 cctgcccgca caaaagccaa caattgccgc tgccctaccg ataattgtgt gccgcgttcc
     8521 tgcacattaa aatcgtaagt gcccggcagg cgctctataa aacgattggc accaacttcg
     8581 gtagcagcaa gctttacatc gttaaggctt atatcttcat tatagagtgt gatattattt
     8641 ttgatagtgt ccgaaaacaa aaaaacatct tgttgcacca atccgatttt tgttcgcagc
     8701 tggtgaatat caaactcctg tatattgatg ccatcaatta aaatttcgcc tttctgtatt
     8761 tcgtaaaatc tatttatcaa tccaatgata gaagttttgc ctgcacccgt agggccaacg
     8821 aatgcaacca cctcgcccac tccaactttg aaacttatgt tgcgcaaaac ccacttacta
     8881 ttctcatcat cggcattata tttaaaccac acatttttaa attcgatatt gcctttcaat
     8941 ttatcggcac tcaatgttcc attctttaca ataggctttg ttaaatttaa aagtttaaaa
     9001 atacgctcac tactaaccat tcccatttgt aaggtattaa acttatcggc caattcgcgt
     9061 atgggccgaa acaacaaatt gatgtacata ataaaagcaa ccacaactcc gaattgtaat
     9121 tcatgatgca agattgcacg ggtgccatac cacacaatca aaccaatagc ggtagcggac
     9181 aacaattcta ccaccggaaa aaaaatactg taataccata ccgatttaat atttgccgtg
     9241 cggtgctcgc gatttatact ttgaaatttt ttgtactcct catcttctct gttaaacact
     9301 tgaacaatgc gcatgcctgt taaatgttcc tgcacaaagg tgttgagatt ggaaacagcg
     9361 gatcgaacat cgttgaaaga tttttttata ttcttttgaa aaacacgtgt ggcgataatc
     9421 aaaaccggca cagtactcaa cgatatcaaa gccaaacgcc aatcgatgta aaacataaac
     9481 gcgataatgg ctaccaactg caaaaggtcg ctgataataa ccaataagcc atcggtaaaa
     9541 atatcggcta ttgtttccat atccgatacc aatctggtaa tggtaatgcc aatgggtgtg
     9601 gtatcaaaaa aagtgaggtt aaatgattgt aatttactga ataaattttt tcgcaaatca
     9661 gcaataatat tttgccccag caaatttgta tttgaggcat aaaatatttg taaaaaagaa
     9721 ttgagcaaag ccgttatcag catgagtgca gtaaaaagca atagatgatg ttcatcgcct
     9781 ttggcaatat aattatctat agttagttgt gtaagccacg gacgtaaagg cgatacaatt
     9841 gccaataata cagaaagaaa aatagtgccg ataaaatttt gcctgtaagg ttttacatat
     9901 cgaaaaacat tcaacaatcc atccagcttt ttctcctcct tattacccat tacttaatat
     9961 ttgagttgtt ttctgtttgg ccaataatta ccggcaatct taaaaccggg ttcgaaataa
    10021 aattatattt cacctgtgta aggtacagcc catgagggtt tacactttta cccgcattgc
    10081 gcctgttgtt gctttgtatt atttcttcca aattatttaa tgtaattttt ctgttgttca
    10141 cattcatcat ggtgccaaca atagcacgca ccataccgcg caaaaatcta tcagctgtta
    10201 ttgtaaaaat tgtaaattct ccacacacat accattcagc tttttcaatt ttacattcaa
    10261 atgtttttac ctgagtattc gatttgcaaa aacattcgta gttagtatat cgatataata
    10321 tctggcaaca ttcattcatt aaacctaaat caattttact aggcatgtat gccgacatat
    10381 tatttacaaa aggatttttt gcgtgatgaa taatgtaatg atatgttcgt gatgtggcat
    10441 caaaacgtgc atggcattta tcatcaacat caaacaaatt gaaaacacaa atgtcaaacg
    10501 gaaggatagc atttgtttga aaaataaatt ttgctgtatc gcttatttta ttttccacat
    10561 cgaagtgaat ataaaattgt gtggcatgca ctcccgtatc ggtacgcccg caacctaaac
    10621 taacagtagg tttgccaaca actttgccaa tagcctcgtc tacaagttgc tgcacactta
    10681 tagcattatg ctgtatttgc caacccgaat attttgtacc attatatgct agttctaaaa
    10741 aatatctcaa tgcatttttt ttgaagggcg ctaaaatcga tgataataaa ttaatatact
    10801 aatggtttta attttgactc cacaccgaca agtaattttt gccacaaaag cactaaggca
    10861 caaaatttaa ctaattcgca aacaaatcat ttttatattt tgtgggattt ggtgatatag
    10921 tggctattta atttatacta agagtagtta gattcttgaa tgaaaatata acgtactctt
    10981 agcaatgccg aacaacttaa atagtttgtt tttccaaaac ctaagttgtt tcggtatata
    11041 agacttttcg gaaaggtcta aatgttattc gattatgaaa agtataaatc gaattaactt
    11101 ttttttaacg aggccgaaac tcacactaca tgccatattc acggcacgag tgctaatatt
    11161 gcgctgcaaa aaaaatacct gcatatgatg gacataaaag aaataaacga aaaaattcaa
    11221 caagaaagtg catttgttga tttactgaat ttagaaatcg gcaaatcgat tgtaggccaa
    11281 aaatacatga ttgaacgatt gctgattggc ttgttgagca atggtcatgt attgctcgaa
    11341 ggagtgccgg gtcttgcaaa aacattagcc ataagttcgc tttcgaaagc agttgatgca
    11401 aaattttcgc gcattcagtt tacacccgat ttgttgccgg ccgacttaat aggcactatg
    11461 atttacaacc aaaagaaaga agaattcaca gtacgcaaag ggcctatttt tgccaacttt
    11521 attttggccg atgaaataaa ccgtgccccg gcaaaagtgc aaagcgcctt gctcgaagcc
    11581 atgcaggaaa aacaagtaac cattggtgaa aacaccttta aactcgaaga accttttctc
    11641 gtgcttgcta ctcaaaatcc cattgagcag gaaggaactt atccattgcc cgaagcccaa
    11701 ctcgatcgct ttatgatgaa agtagtaatt ggatatccag gtaaggaaga cgagaaatta
    11761 attatacgcc aaaatctttc gaacgaaaaa acaaccatta atcctgttgt aaataccgaa
    11821 agtattttga aagcacgcag cgctgttcgc gaagtttaca tggacgaaaa aatagaaaag
    11881 tatattgtcg atattgtttt tgcaacacgt tttccaaaag aaaataaatt gcaaaaactg
    11941 gcgcaactga ttaactatgg aggctcgcca cgcgcaagta ttaatcttgc attgggtgca
    12001 aaagcgtatg catttataaa acgcagaggc tatgttattc ccgaagatgt gcgcgccata
    12061 tgtcacgatg tgatgcgcca ccgcattggt cttacatacg aagccgaagc cgaaaatgta
    12121 acaacagagc aagtaataaa tgaagtgctt aatatggtgg aggttcctta gggattgtca
    12181 ttgggcattg gtcaataggc attggtcatg gtcactctga gcgaagtcga agggtcattg
    12241 ctgcgcaagc catggtttgt gtcctcacaa accatttcga tgcaagttgg tactcgtcac
    12301 aaaaaattgc tccgtcagcc attgtttgtg tccactacgt cagccattgt ttgtgtcctc
    12361 tgcgttagcc attgtttgtg tcctctgcgt cagccattgt ttgtgtccac tgcgtcagcc
    12421 attgtttgtg tcctcacaaa caattcttgc gcactagcac accggcatag aaaaaattgt
    12481 tttgttgaaa taaatgcatt aaaaaaaata ccatcaagaa atttgtgtaa gaagaaacag
    12541 aaacaaaaat tacttcaggt aaataataat tacaaaaagt ggaaacgcaa gagcttttaa
    12601 aaaaagtaag gaaaatagaa attaaaacac gtgggctctc gaatcaaatt ttttcgggcg
    12661 agtaccacag tgcttttaag ggccgcggta tggcctttag cgaagtgcgt gagtatatgg
    12721 ctggcgatga tatacgcagc atagactgga acgttacctc gcgcttcaat catccttatg
    12781 taaaagtgtt tgaagaagaa cgcgaactta cagtaatgtt actggttgat attagtggca
    12841 gtggcgaatt tggaacacag caacaattca aacgtgaaat gattacggag ttatgtgcgg
    12901 tgctttcgtt tagtgctata aaaaacaacg acatggtggg ggttgttttt tttagcagca
    12961 agattgaaaa atttattccc cccaaaaaag gcaaatcaca tatcctgcgt attatccgtg
    13021 agttgataga cttacagccc accgaaaaag gtaccgacat agcaatggcc ttgcgctact
    13081 ttacaaacgt gataaaaaaa cgcagtatat catttatcat ttccgatttt atgagcagca
    13141 attatagcga tgcatttaaa attgccgcgc gcaagcatga catgattgca cttaaaatat
    13201 ttgatgaacg cgaacagcat attcccaata ttggtatcat acaaatagtt gatcccgaaa
    13261 caggcaaaac cctcacggtg gatacaagca gtgtggattt gcgtaattct tactcacaac
    13321 attttactaa tgcaaacaaa gaatttagca acctcacagc aaaatgcggt atcgactttg
    13381 taaatttacg taccaatcaa aattacattc aaccgttgat gaatcttttt aaacaacgtg
    13441 aaaaaggaag aagatcagta tgatgacatt taaaagattt cttactccaa aatatttttc
    13501 tgcttcagtt gtggcgatgt tttttttgtg cctcaatttt aatttaacag cacaacctca
    13561 gcaacaagtt gcagcaacat tagatactaa cgtaattcgt attggagagc aaaccatgtt
    13621 acgtttgtca tcggcattca acaagaataa taaatacaac tggtttgcat tgcccgatac
    13681 atttaataac atagaaatta tcagccgcga tactgttgat accattcagg taaacaatga
    13741 ctggcagttg caacaaaaca ttcttatcac atcattcgat agtggtaatt gggttatacc
    13801 acctgcacaa ctttcgttca atttgccggg cgataccacc acctattttt caagcacttc
    13861 gcctttgctc attatggtta atggcattgc tgttgacact tcaaaggcag cttacgatat
    13921 aaaaagtgtg attgatgtgc catttagtat catggattat atttggtgga tagtaggagc
    13981 aattctgatt cttgccatca ttgccggcac tattttcttt ttacgcaaat cgaaaattga
    14041 gcaaggtgca cctcaaatag tgaaaataaa aaagcctgca cacgaaattg cattggcagc
    14101 actcgcaaat ttgaaagaag aaaaactgtg gcaacaaggt caggtaaaag aataccacac
    14161 caaccttacc gatattatac gaacatatat tggcaatcgg tataacatac atacactgga
    14221 acttacaacc gatgaaataa tgcaatatca aattataaaa tcgttgccag cggatgtaaa
    14281 tttggaattg aaaaatttgt tgcaattagc cgaccttgta aaatttgcaa aagccaaccc
    14341 acttgctaac gagcatgaac tgagttacag cagtgcgttc gactttgtga tgaaaacaaa
    14401 acaaagcgaa caaacacctg taataaaaac agaacaaaac aattcaacgc aaaaatgata
    14461 agttggtgga acgatataca atttgctcat ccatattatt tatggttgct attgttgttg
    14521 ccaatacttt tgttttggca tttatttttt cgcaaaaaaa acattacttc ttttaatttt
    14581 tcgaatacaa aaccatttga aaaaacgaat agaagttggc ggcaatattt attgaatgta
    14641 cctatcgtat tgcgcatgct tactctcgca tttattatag ttgccatagc tcgcccacaa
    14701 tcatcatcgc gagcgcaaaa cgttaccacc gaaggtatat ccatagtact tgctctcgac
    14761 atatcggagt cgatgcttgc cgaagatttt aaacccaata gaattgaagc cgcaaaaaaa
    14821 gtagccatcg attttattga aggccgccct accgatcaaa ttgggttggt ggttttcaag
    14881 ggcgaaagtt tttcttcgtg ccccatcaca accgatcatg gtgtactcat taatttactt
    14941 cgaactataa acagcgatat gttgcctaca cccggcactg caattggcga aggacttgct
    15001 acagcaatag cacgagtgaa agatgccaaa acaaaaagcc gtgtagttat tttacttacc
    15061 gatggtgtaa acaattcggg aagtatagcg cccgaaactg cgggagaaat tgccaaaact
    15121 tttggcgtgc gcgtttatag tgtaggcgtg ggaacaaatg gctttgcccc tactccaatg
    15181 aaaacaccct ttggtgtgca ataccaaaat gtacctgtac aaatagacga agacatgctt
    15241 cgcaaagtat cgaaagaaac agatggaata tattaccggg caaccaacaa caaaaagtta
    15301 cgcgatatat atgcacaaat agataagctc gaaaaaacaa aagtggaagt aacagaattt
    15361 cgcaggtata gcgaagagta ctggccactg gcagttgtag caggaatttt atttcttctc
    15421 gaatttattt tgcggtattt agttttgaaa aaaattcctt aactgtaatg ttgaatttaa
    15481 gtttgtataa aatcacgttc aaataataat tgaaaaaagc gttgataaaa aaaaagaaaa
    15541 ttagtctcgg attagaaaaa aataaaaaag cataatggaa gagtttcgct gggtaaacga
    15601 aaatttattg tatctctaca tactactgcc gctgatggta ttgtgcttct atttcatgta
    15661 cagatggaaa aaaaatgcca tcaataagtt tggacaatgg acagtaatag cacccttatt
    15721 tgcgcatgta tcgaaaataa aacctgtgtg gaaatttgta ttcatgtttc tggcggccac
    15781 tatgttaata acagcaatat gtcgtccgca acttggaagc aaactcgaag aagtaaagcg
    15841 taaaggagtg gatattgtta tttgcctcga tgtatcgaac tcgatggatg ccgaagacct
    15901 aaaaccaaac agactggaac gagccaagca ggccatttcg caattggtag acaggttgca
    15961 gggcgataga attgggctca tagtttttgc cggtgaatca tatactcagt tgccaataac
    16021 taccgactat ggcgcagcaa aattaatgct cggatctatt ggcaccgata tgattcctac
    16081 gcaaggcaca gctataggaa gcgctataga ccttgctgca tcatcgtttg gtgatagcat
    16141 aaaaaaacac aacgctgcca ttgtagtaat taccgatggc gaaaatcatg aggacgatgc
    16201 aattgaagca gcaaaagaag cttacaaaaa aggtatcaca gtgcacacaa ttggtatggg
    16261 ttcggtagcg ggtgctccta ttcctataaa acaatatgga caaaataccg ggtacatgca
    16321 ggacgaaagc ggacaaactg tactcaccaa acttgacgaa aataatcttg tacaaattgc
    16381 cgatgccgga ggaggaaaat ttgtgcgcgc tacaactagc gatgatggct tgccacttat
    16441 tatgaatgag atagacaaaa tgaacaagaa agaattttcg tcaaaaatgt ttacctcata
    16501 cgaagagcag tttcaatggg cattaatcat tgcattattt tttcttatta ttgaattttt
    16561 gataagcgaa cgaaaaagta aatggctcga aaagttgaaa ttgtttgaag aagaaaaacc
    16621 agtagtgtaa tgtttttaat aaaaagtaaa atgaatacaa aaacacatat atgttgtaag
    16681 ctcatcatga tggttttatt gttgatctta caaattaatc tggaggcaca aaccaatgcc
    16741 accgaacgta aacttgtgcg cgagggcaac aaaaaatata tagaaaaaca attcagcgat
    16801 gccgaaacta tttatcgcaa agccatgcaa accgaacatg gaagcccaac cggaacgtat
    16861 aatttaggaa atgctttgta ccgtcaaaaa aagtacgatg aagcagtaca gcaataccaa
    16921 tcaacaataa acagtaaagc aaacaacgaa agcaaggcga aagcatatca caatttaggc
    16981 aacacgtatt tggagcagca aaaatatcag gagagtgtgg atgcatataa acaagcacta
    17041 aaattaaatt cgaaggatga gcaaacccga tataattatg cgtatgcaaa aagcaaatta
    17101 gtgcaacagc agcaacaaca acaacagaag aaagacgata agcaacaaca aaagaaagac
    17161 gacaagcagc aagagcaaaa acaagacgat aaaaaagagc aggataaaaa tcagcagcaa
    17221 caacaacagc aagataaaga acaagagcaa aaaaagaaag aagagcaaca gcagcaggcg
    17281 cagcaaaagc caaaaatatc gaaagaagat gccgagcgaa tgttacaggc tttgaagaaa
    17341 gatgaaaaag atttacaaaa gaaaatgcac aagaaggaag gcacacgttc aataatttct
    17401 aaaaactggt aaaataatat taagccaaaa aaaatgaaaa tcaactcctt gtataaatat
    17461 tttgcaacaa caattatatt gtgtgcatta actgtgagta tatacgctca acaatttaat
    17521 gccactatca acaaaaccaa tgctgtggta ggcgaacaat tgcaaatcag ttttactttt
    17581 aacgggcaag gcaaaaattt tggccaaccc gattttaaaa actttcaggt gattgccggt
    17641 ccttcacaaa gcagcaacat gacaatcatt aatggcaata tgacctcatc acaatcgctc
    17701 acatattgga ttgtaccgca aaaagaaggc acatttaaaa ttggccctgc caccattacc
    17761 atagacggaa aacaaattgc gagcaatgtg ataactgtat cagttggaaa aggtcagcaa
    17821 caacaggcac aaggcaatca gcgacagcaa caacagcaaa atgaaagtgg catcacatcg
    17881 aaaaatgttt ttattaaaac ttcaattgat aagtcatcgc tctatcgtgg tgaagctatg
    17941 gtggttacat ataaattgta taccaatgtg agccttgtaa atctcattcc taccgaacaa
    18001 ccttcgttta acggtttctg gaacaatgat ataaagttac ctgcgcagcc aaactttgtg
    18061 cgcgaaacat acaacggcac tgcatacaat gctgcagtta tttataaagc cgtgttgttt
    18121 ccgcagcaat cgggttccct cactatcgat ccgtataagg ctaccgttat agcgcgtgta
    18181 caggtaaaac gcaatcgcaa tcctaatgat cctttcgata tttttaacga ccctttcttt
    18241 ggcggtggcg cacaggatgt tgagtataaa gtaaccggtg aaaatataaa agtgaatgta
    18301 aaggaattgc caccaaatgc acctgcatca ttcgatggtg ctgtagggcg attaaactat
    18361 gacgtaaaat tagataaact gcaaaccaaa gcgaacgagc ctcttgcatt gaaagtgaaa
    18421 atatcgggac gtggaaattt acaattaata gatgcaccaa aaataaatgt gccggccgat
    18481 attgaaacgt acgatcccaa agtagccgat aactattcta taaccgaagc tggtacaaat
    18541 ggaagtaaaa catttgaata cctgctcatt ccacgcaacg agggcgaata tattattgat
    18601 gccattccat tttcttactt cgatttagaa acccgtacct accgaaccag caatgccggg
    18661 ccattcaaaa ttaaagtgct caagggcgaa ggtggtgcag caagcatttc gaacagcaca
    18721 gccaccattg ccaacaaagc cgaagtagca gtgctaggcc acgatgtacg ttttataaaa
    18781 acaaacatgg gcgaagtatt aaacatcaat caattatttt atggatcatt aaaatattat
    18841 tttttgcttt tatttccttt tgcgctttcc atagctgcca tatttttgaa atcgcgcaat
    18901 acaaaactta acagtaatat tacgatggta aaaagtcgtg gtgcaaataa agttgccact
    18961 aaacgacttg cacttgccgg taaattatta acccaaaata aaaataatga aatgtatgac
    19021 gaaatttata aagctattaa tacttatgta agcgataaat ttgccatacc aatttcggaa
    19081 ttaacacaag acgaaatggt atctaaactt acattgcgca aggtaaatac aacaacaatc
    19141 gaatcgctca ccggagtatt gagccgttgc gaaatggcgc gctttgcttc gggtgttgca
    19201 gccgatgcgc aagttgtttt caatgatgct atgaatgtga ttacaaaaat cgaaaatgaa
    19261 actgtttaat ataaaaagag tatttatttt tttcctgctt gctttatggc ttggccatga
    19321 agtaatggca gcagaaacta aattgtttga gcaggccaat actttttaca aaaataaaaa
    19381 ctacaacaag gcaattgaac tgtataaaaa aataattgcg aaggataatg catcggcagc
    19441 agtgtatttc aatcttggta atgcttatta caaaacaagc gaaacagggc ttgcaatttt
    19501 aaactacgaa cgagcattaa aattacaacc caacgatgat gatatagaat acaatctgaa
    19561 acttgccaat atgcaaacaa cagataagat agaaccggtg ccccaggttt tttatcagcg
    19621 ttggtggacc agtcttatta ctgcaatttc acttagcaaa cgcacattgg tttcaataat
    19681 tcttgcctgg ttatcgtgca ttttttttat tgcttattta tttatccgca acatgcagct
    19741 caaaaaaata atgtttgcat tggcattaat gctttttgca ggattgtgtg taaatgcttt
    19801 tttggcatcg caacaatatc agcaggtaaa tcaaaaacgt tttgcaatta tccttgccac
    19861 aaacccatac ataaaaagct cgcccgatga aaaaagtgcc agcttgttta tgctgcacga
    19921 aggcacaaaa gtagaagtgc ttgacgaact tattggttgg aaaaaaatac gcatagccaa
    19981 tggcaatgtt ggctggattg ctgccgatgc actcgagcaa atataatttc acgctttcac
    20041 tttcattggc aataccatag ctgtttatgt attaaagagt tcccatttat cgttttgcag
    20101 cttaatggtc aggcaagtat tttttttttg aaaacctgcc ggcatctgtt ataatattta
    20161 cttttggcca ataaaatttt acagatgaaa cttcttgccc cttccattct ttcggccgat
    20221 tttgctaacc ttggcaaaga tgtgcttatg tgcaacaaca gtcaatgcga ttggcttcac
    20281 ctcgatgtaa tggatggtgt gtttgtgccc aacatatcgt ttggtttgcc tgtgataaag
    20341 gccataaaaa aacatgccac taaaccgctc gatgcccatt tgatgatagt aaaccccgat
    20401 ccatttatca cgcattttaa agatgctggt gccgacaatc ttacagtaca ttacgaagct
    20461 tgcacccacc ttcaccgtac tgtagatggt ataaaaaaag ctggaatgaa agccggagtt
    20521 gccatcaatc ctcacactcc tgttcatttg ttgcacgata ttattgccga tatcgatttg
    20581 gtatgtgtaa tgagtgttaa cccgggattt ggaggtcaaa aatttattga aaatacttat
    20641 tcgaaaattg aagcactggt acaacttatt caacaacgaa attccaaagc acttatagaa
    20701 attgatggtg gtgtggatat gaataatatt aaaaaattat acaccacagg tgcaaatgta
    20761 ttagttgcag gcaatgctgt attctctacc gaaaacccaa ctcaaaccat tgctgccatg
    20821 aaaggtattg tgtaggttat tgatttaccg aacgcagtaa gcgaaatatt ttttcaaaag
    20881 tttttttggt cttgtattga aaaaaagttt cataaaagag attgaaaaaa gaaataaatc
    20941 cggcaaaaga agttatcact tacttcaaaa aaacaggtgc aaaaaaagca cctcgaaact
    21001 tttccttcaa aataatattg cgcacttttt tcgatacaag aattggtcgc gaggcactgc
    21061 ctccgcttcc aaaccattca tatggtttta caaaatcggg ttgcccttcg aatatatttt
    21121 tgtcaaaagt caaagcgcct ctttgtggaa agttgtattt caattgtcca caataatttt
    21181 tcgggctatc cacttctcca cttgattccg cattgtcttt cttttcctct ttgtgcggtt
    21241 tattgcaaat ctcaacattc aaaccatcag gaaccaagcc cgtactaagt acagtatcaa
    21301 catgcaattg ctgtatcgat tctattggtg tatgtgattt gctcaccacc gggttcgaaa
    21361 atcgcatgcc tgtaacattt gcatggcgaa aaatatttgc tgcaacatca cgcacaaaca
    21421 tttcatcgta tacccaatgc aaccctaaga attgactctt tcccggtttt ggttcagtct
    21481 taaaacgaaa aggattcttt tgccttttgc ctatttcaca tttaaaacaa acattatcta
    21541 ttgcataagt agtttcaaaa aatttgcctt cgggctgcgg ataacctatc ggagcaacgg
    21601 taaaccaaaa ccactcggcc ttatcgaaat cttctggaga aaattctgtt gttacctgat
    21661 gcaataaagt gaaatgctga accagatttt gtttaaacgc aaaattgggt tcgtcttcgt
    21721 acaaaaaaaa ttcgaggcta taacttttta aatcttcatc gtaagttttc tcggtaacat
    21781 ttattttaat ttgattcgtt tgcaaaatct ccaatagata gtcattgcgc tcaaaggtta
    21841 aatatctgtg tgctattcgc attcttaaaa tttaataaca aagctataaa aaaaggaagc
    21901 ttatgttcaa ataaaatcgc aattgctttg taatgatttt aattcaactg tttcaaaatc
    21961 caatcgaggc aatcttgcga atccataata ctccaagagt gtggatgttt ggttccatca
    22021 atccttacac ctttgtcgta ggttacaatc atttcggcat tggtgttatc gttggcttga
    22081 ttataccgaa acaacatagg ttttggagaa acaatctatc aagttgttcg gtattactaa
    22141 gcgtacgtta tattttcatt cgaaaatcta actgctctta gtataaaata agggtattca
    22201 atgtgcataa attaaattga aaaaaaatat tagggattct ttcatgcttt tcgtggcaac
    22261 aaatactttt ctgaggcact caatattaaa ggattgaaac tcccaccaat aaaataagag
    22321 cttaggataa atttatattt ttattacatc aaaactattt ctcaatgggc aacaattatt
    22381 ttttgcgaaa aaatattttt ttctgtttgc gcttttacta tgtaaacgcc tgttggatgc
    22441 gaactcatat ccaatgattt tgattcgcca attaaatcaa acgcgaaaat ttgctcatca
    22501 ataatattat aaatgctaac tgtagtttta ctttttgtga gggcattaat tgtaaataac
    22561 ccatccgaaa cagacggcaa tatttgtaat aagtttgtta gttgtgattc ttcaattgct
    22621 gtactccatg atagccttgc aacatcgtgt gccactttgc ctccaaccga actaaaaaag
    22681 ccactaatgt acaagtcatt atttaatgtt gtaaaattgt gaacatcacc ccaatagtta
    22741 aatcctaatc ctgttgtcca gtttgtgcca tcccattcac ataggtatac atagttgttt
    22801 attacttcac gacaatacaa tttgttgtta tattcgccta tttgctgcgt accgggaata
    22861 aattttgtac ccacttcgga ccaatttgta ccatcccact ttgcaatatt atcgcccggg
    22921 tcgccatgtg ccttgtcgaa cgaacctgca gcatacaatt cgtttttgta aacgactaaa
    22981 tcattaatgc ccgcccaatt tgaaaatcca ttgcccacag gattccaatt tactccatcg
    23041 aaccgggcaa ttttacttat ttttgaatta taggggcttg taaatccccc agctacatag
    23101 agttcccctt tgtacatgat gcatgatttt ataatgctgc cattttgcaa tgaaaatgaa
    23161 tccaatggag gaaatgcaaa ccaattgacc ccatcaaatt ttgcaatcat gtctgcctga
    23221 acgccaccga tagtatcgaa tctgcctgtc acatacaatt cgttgttgga tacatacatg
    23281 ttttccacaa aatcatttag caaggttttg caactcccgc ctttgcaagc tgaaacccat
    23341 tttgtaccat cccattttgt taagcaatcg tatatccatt gcccgctagt atctaaatat
    23401 cctgccgcac ctgcatatat ctcacctttg tagctcgaaa tactgttaac aatgccaatc
    23461 ccaaatgtaa ttccaataga atcccattga ttaccatccc atactgcaat atagtttata
    23521 ttttttccat ccatttgtgt aaatgttccc gaagcataaa ttttatttga tgaagtatca
    23581 gcaaataggc aatgaatttc atagttgact cctgaaccca tcgcgtatac cgtttgtgtt
    23641 ttggcaattg aaaataaatg actcaacata attattataa ttaatttttt cattgtgtaa
    23701 ttatttgcgg attacaattt tgaaacaaag ctataaaaaa aggaagctta tgttcaaata
    23761 aatttataat tacccggtta agttttcaat gtaaatgttt caaaaagcaa tcgaatcaat
    23821 cttgagaatc agtaatactg gctgaatgtg catgtttggt tgtatctatt gtcaccatgt
    23881 taaaacagta tttatttact tcagcattgt tgctgccatt cattttcaat agttataatg
    23941 ttgcgataat tattacatta aaccgatgtt taataaatta tttatacatc aagaaatcaa
    24001 aaaagctaca atcattcagt ggcatcacag tgtcgaaaat agaattaata ttttttgaat
    24061 ggtcaattac atcattttat ttttcgtgca agtaataaca aatcaataac cttggttgca
    24121 tcattagttg ggtatggttg gtgttgcatg tcgataattt caaaattgtt tttcgtgagt
    24181 gcatcacaca aataatcggc ctgatgataa tgcataaata tttcatcgcc tgtactgcct
    24241 tttacatatt gcgacttttc ataatcgtct tccatggtgc tgataaaaaa cacaccgcca
    24301 ttttgcaaca atactccggc atcgtctatc aactttattg cttcgggctt tgatagataa
    24361 ggaaaacaaa aaccgcacat aatggcatca tacttttcgg gaagcgaatt aattgctcga
    24421 caatccatca cacgaaaatt tgcttgtgga ttatttttct ttgcgaggtc aatcatattt
    24481 tgtgatagat cgattcccaa tattttgaag tcgggtctct tgtatagtaa atactttgtg
    24541 atgttacccg gcccgcaggc aatgtcaagc acttcggcat ttggctttac aatgttatca
    24601 caaaacatat cgaatgcatg atggtacaat tctacattca taaatttatc ctgatacaac
    24661 aaagcatttt tatcaaaaac ctcagcagca tgttttgatt tgtcgattaa catagcagaa
    24721 aatattttga attattattt ctcaacaaag gaaagaataa ataaaatgga aggtgattaa
    24781 ttgatttcgg taacgcatac aacaaaacat cgcgaaagat tactttgaaa gtaagagtgc
    24841 aaccatcaaa ttaattaagc caccaaatag ctccatttag aatagaagca aaagtaaccc
    24901 atagcaaata aggtatttga agtgctgcag ctgttttatt tattttcgaa aaaataataa
    24961 tcattgttac aatgcttaac catatgcaaa taatttcgac caaggcccaa cccaaaagct
    25021 gatacttgaa aaatataaaa ctccagcaaa aatttaaaaa caattgcaca agaaatattg
    25081 ccacagcatt ttttctgact acacttgcag gtgcttgcaa aaccatataa aaagaaatgc
    25141 ccatcaaaat atataatgca gtccataccg gcccgaacaa ataatttggt ggattaaaga
    25201 aaggtttgtt caaagccata aaccaaccat taatacccga tgctgttgcc acacctgata
    25261 ctgcgcccac acacaatgtg aaaagcacac ataacaccag cgacaaaata tttttcgctt
    25321 tcaatttcag gtttttactt tgtaataaat tttccgaatt ttttttcaac tgcaatggtc
    25381 cgaaaatcga aaatttgttg caactgtttt ttaatgaaaa gagtatttgc tattgcaccc
    25441 ataaaaccta atggtggctg gtaagtaaca atatctgtca taagcactcc cccttcgatg
    25501 gctacaattt tatgttggtg atgccacaat gtgtaaggcc caattcgttg ttcgtcaaca
    25561 aaatactcat gctcttttac ctgcgttatt tctgtcatcc aatctaattt tattcctaac
    25621 aaagggctca ctttatatgt gataatcata ccgggataca ttttttctgc accggtattg
    25681 ctggtaacaa caaaacccat gtgaggtggt gtaatttctt tgaggtttac aggagatgaa
    25741 ataaaatccc atatcatcgc aatggatgca ggaatttttt gtgttttttc taattgataa
    25801 aatgccatat atgataaatt attttattgt tgacatacca ccatccacat gaaaaatttg
    25861 acctgtaatc caggccgctt tttccgacag taaaaattct gccatgtttg caatgtctgt
    25921 tgctgtgcca atttttttta acggatgtcg caaagcattt gcttcacgtt tttgttcgct
    25981 gtttaataat gccgctgcca ggggagtatc ggtaagtgat ggtgcaatac aatttactct
    26041 tataagcggt gcatactcgg cagccaatgc tttggttaag ccttcgatgg cgcccttcga
    26101 tgctgcaacc tgcgaatgga aagcaagacc cgattgcact gcaactgtag aaaataaaac
    26161 tatagaggca ttttccgatt tttttaactt tggtaatact gattgcaaaa cctttattgc
    26221 tcctatcacc tgcaatttgt aatcggttac aaaatcatcc ggtttaattc tttcgaaggg
    26281 ccgtaaattt atacttccgg gacagtatac aacccccgtt atatgttcgg gcaaaaaatc
    26341 cattaccaaa ttttcgtcca acacattgag gtaattatac tgcaaggaat tttgttcgga
    26401 gtgtgtttca ttcttacaat atgttccata cacctgatgc cctgaggttg ataattgttg
    26461 ggctaccttt ttgcctatgc ccgatgatgc tcctattact atatagttcg acataaaatt
    26521 gatttaactt ttcaatgcaa ccacaaacaa caaatgccat gtttagtttg aaaaatctaa
    26581 aaacacaatt ttaaaaagta tttgaatgat gagttcgcaa cataggtata caaaaaaagg
    26641 cttgctcata ttttcggaat ttaaataaaa ttatcaaagg ttagtacatt caaaatttcg
    26701 gtaataacta caacataata aaatgaggta tgccgcaatg atgaaatatt ttatcaggtg
    26761 cggactaagt agctaatcaa tccttatcag attttgcgag tattatgaat gattacattt
    26821 actgatattt gattgttact gtacttcaaa aaaataaatg tctatcgttt tagaaaattt
    26881 cgaatagtgc tttttaagag atgaactatg gcattaatat tttaaaacaa attttacttt
    26941 ttggttggtg ctgatacatt atatttaatt caattgcttc atatataatg agtagttaga
    27001 ttttcgaaag aaaatataat gtactcttat taatgcagaa caacttcaat agcttgcttt
    27061 tcaaagcata atttgtttcg ttataacaat gagtttttga tagatcacgt acagagagtt
    27121 tttattttca agcatagttt aaaaaattgc gtcttgagaa atcgcaaagt tgaacaaaac
    27181 tttttgttta atatttttta ccattagtta aacaatatca ttttgtgctt gttgtgattg
    27241 tattctcaaa caaaaaaatt aaaaaattaa aaaaatgaaa aaacaatttc taaaattgat
    27301 cttgacagga gccttaggat taggctcaca attaggattc ggccaagccg aacttcaggt
    27361 aattcacaat tctgccgacc ctgcagcagc aacggtagat gtttatgtta atggtgcatt
    27421 aacattagac gatttcgcat ttagaactgc aacacctttt gttacactgc catcgggagt
    27481 gcaattaaat attggtgtgg caccgggtaa cagcacatct gcaaacgata cattgaaaaa
    27541 ttttcaggta acattgatga tgggtcagcg ctatgtagct gtagcgaatg gcgttcttaa
    27601 tccgggtagt tttgcaccaa atcctgatgg cacctcttcg gcctttacat tatttttaca
    27661 aaacaacatt aaaaacatgg ccacgaatgc aggcgaagta gactttgttg ccgttcatgg
    27721 ttcaagcgat gcgcctactg tagatgtaat agcgcgcaat gtggcaactt tggttgataa
    27781 cgcctcatac tcaaatataa caggatatat aagtgtaccc gctgcaagct atacacttga
    27841 tgtaactcct gctgcaggtt ctccaattgt agcatcattc acagcagatt taagcacctt
    27901 aggtggtggt tcggcaatag tatttgcttc gggattttta gacccatcga caaatcaaaa
    27961 cggaccggca tttggattgt atgctgcact tgccaatgga actgttgtag cttttccatc
    28021 ggttagtttt gcacgcttac aggtaataca caatgctgcc gaccctgctg ctgcaagtgt
    28081 tgatgtttat gtaaacggaa atttactttt agacgatttc ggatttcgca ctgctacacc
    28141 ttttgtagat gtgcctgccg gagtgctact taacattggt gttgcaggtg gaaacagcac
    28201 atcggtaaac gatacattaa aaaactttca ggtaacactt gccaatggtg gtcaatactt
    28261 agcggtagca aacggagtac taaatccggc aaactttgct gcaaatcctg atggaaaatc
    28321 aactgccttc acattatttt tacaagacat gatgagagag tcggcaatga tgagtaccga
    28381 tgtagatttc agagtaatac atggcgccag cgatgcacct actgtagatg ttattgcacg
    28441 taatgttgca accattgtta acgatgcgcc ttatagtgca atcacaggtt acatcagtgt
    28501 tcctgctgca aactatacat tggatgttac accggctgcc ggctcaccta tagttgcgac
    28561 ttttaaagca gacttaagta gcttggccgg aggttctgct gttgtatttg catcgggttt
    28621 cttaaatcct gcaacaaatc aaaatggccc tgcgtttggt ttatttgctg cgcttgcaaa
    28681 tggtacaatg gttactttgc cacaaacaag tatagcacgt cttcaagttg ttcacaatgc
    28741 tgccgaccct gctgctgcaa gtgtagacgt atatgtgaat ggtaatttac tgctcgataa
    28801 ttttgcattc cgcactgcaa ctccttatgt tgatgttcct gccgatgcgt tattaaacat
    28861 tggagtagcc ggaggtaaca gcacttcggt aaacgataca ttgaaaaatt tccaggtaac
    28921 tcttgccaat ggtggtgagt atcttgcagt tgctaatggt gtattaaatc ctgcaaactt
    28981 tgctgcaaat cctgatggaa aatcaacagc atttacattg tttttacnnn nnnnnnnnnn
    29041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29101 nnnnnnnnnn nnnnnnnnnn nnnnnnngca aactttgctg caaatcctga tggaaaatca
    29161 acagcattta cattgttttt acaagaccaa atgagagagt cggctttggt gagcaccgat
    29221 gttgatttaa gagttgtaca tggcgcaagt gatgcaccta cagttgatgt gttggcagga
    29281 gcaagtatat tggttgacaa tgctgcttat ggcgatatta ctccatactt gtcggtacct
    29341 gctgcaaact atacacttga cattactccg ggcaataata actctgcaat agttgcaagt
    29401 tttgctgcga cactttcagg tataccagga aaatcggctg tggttattgc atcaggattt
    29461 ttaacaccat cggcaaatca aaatggtgct gcctttggtt tgttagctgt gttggccgat
    29521 ggaacagcat tcatgcttgg caatgttaca agtattgacg aaaacaccgg cacaattaat
    29581 tttggtatgt atccaaatcc tgcaaacgat atggtaatga tttcattgcc cgataacgaa
    29641 acaaatgcta ttgtagaaat aaccaattcg ttaggtcagg ttgtactttc acaacgccca
    29701 aacaattctc ttttcgatat ctctttgaaa aactttgtga gtggtgtata ttttgtgaat
    29761 gtacaaacac cagcaggaaa atctacttct aaattaattg ttcaataaat tcaataatca
    29821 aattgctcac aatacaattt gaataaatac tgaaatgctc ctcaatcaaa aatagatttt
    29881 tgtatactta aacaaagctc ctgatgcgtt gcacaaacgt tatcaggagt ttttttttgc
    29941 cggtactttt taaatcaaac tgtaacaact taaattacaa aaatgcaatg ttggtttgct
    30001 gactcatgaa tttattgaaa tcgtttaacg tatatactta tggttaaaat aaataaagat
    30061 tttccattcg ttaactaaac tatactccta ttctacttta aacacaacta ttaaatcaac
    30121 ctctgctttc aattacgaaa aatacccaga atggcatctc gaaaaaatga tgggaatcat
    30181 acaagatatt gacaaaatga ggttgaggaa tatgcattca aaaaaagtaa agagcacaat
    30241 aacatcgttc acaaatcaag cacgggtttt aaaaaatctt taaatttcaa atgttgcgct
    30301 tttatgaatc ttgagggttg aacaataata caggttttga ttttcaatct ccccaaccct
    30361 gttttgattt tggtcagcta gttaaatatt gaattgtcca tttcctatct gcaaatattt
    30421 catactcgtg ctgcacctcc aagagtctct tggtgttagg ataaatccct ttttaacttc
    30481 ctctttcttc actattcgaa gtagattcaa tgatacattg ggaattaatc accagaaata
    30541 ttccaggtaa aaatgctatg cttattttac aaacatagtt tcaatcaaat aataatgcat
    30601 atttacttct tgaagtcgga tgttattttc tactgaacag tttttaatag tttaatttat
    30661 gaaacctcta tcaatagcca atcgaaaaat attctttaat gccatttgct atttcatgat
    30721 tgtggcaatt ttaaatctta cagtgtcgtg tagttattat aaggtaaata aagttcgaaa
    30781 cctgaatgaa gacaaaagca aagttgaagt agaaaaatat cgaaatctta ataaatattt
    30841 tattctgcat acaggaaacg aaatgtggca tgtatccatc caaaacataa acgctgaaaa
    30901 caaaacaatg gaagtaaaac ttgaaagcgt ttcagccaag cacaaactat atttaccaaa
    30961 tgctaccaac agcacgcgca ttcatgaagg ccggggcggg cgagctgtgt taaacgaagt
    31021 gcatattctt acctcacaat atgttgatac acatgacagc attgccacaa tcaatttttc
    31081 ttccatcacc cgcatcgaca taattgaaat ggatacctgg aaaaatattg gcacattcgt
    31141 tttcacagct acaactgttg ctgttgctgc ctacgttata agtctcataa ttataatact
    31201 tacaaaatca tcctgcccgt ttgtgtatgc acacgatggc acaggctaca attttaaagg
    31261 tgaaatatat ggcggtgcta tttttaaacc attggagcgc gatgattata tgcatttgca
    31321 aaacttatca accgatgcaa caaatttcaa aatgaaaata acaaacgaat tgctcgaagt
    31381 gcaatacaca aatttggtaa aagcaatgct ggtggagcac aataaaaacg aaaatatttt
    31441 gattgataac aggggcgaaa tacatagtat aaaaagtacc caaccaccac ttagtgcact
    31501 aatgaacgat accgaaaata tgatgacaca gcttgctgcc atcgatagta atttatgcat
    31561 gttcgatggt gaaaataaca accataatgt gaacgacctg gttttaaaat ttgccaagcc
    31621 tgcaaaagcg caagagacaa aacttgtagt acgggcaaaa aattctttat ggctcgatta
    31681 tgcatttgga gaatttacaa aactatttgg tagctattac aacaagtttg tcgaaaaaca
    31741 acgcaccgcc aatcacgatt cgttactaaa ctggactgtg aaacaagggt tgccgctatc
    31801 ggtttattta aaacaaaacg gacagtggaa attgatagaa cacattccta cagttggacc
    31861 tttggcttcg cgcgattttt gtattcccat cgatgtgtcg caaatcaaca ctcccgatat
    31921 ggaaataaaa ataagttgtg gttttatgtt ttgggaagtt gattatgcgg gcatagatta
    31981 ttcggaagac gaaaatatta aaaccaccga aatggaattg caaagcgcca tagacgaaaa
    32041 caatattgat gtaaaatcgc tgattacaga aaatgataaa aactatttga tacaacccaa
    32101 accggggaat accgttgagt ttatatacaa acttccggca atgaaaaatg aaaaagcata
    32161 cgatgtattt ttgcaaacaa aaggatacta tgagcatgta agaaattaca aggggttgcc
    32221 caataaaaaa tacctgactt ctatgaaaga ggaaggcgca tttattgatt ttacaaaatc
    32281 gttattcgac aaaataaaaa acgatgcagg ccttaccact tcgaaataat taattatgag
    32341 tacaatcaac aaaactgata ctttggaaaa taaaattaat gcgcaaagca ataatattat
    32401 ttctgtaact aggcgaaatg ctacacatat tattacgggt tataaagcaa ggttacaaag
    32461 ggtaaaattt cgtttacata ttttaaaact tgcattgcaa acttatggat ggaaacgctc
    32521 aacttttagc atgctcaaac aattggaaca aaaacgaaga atagtgcatg gcgaatttcc
    32581 aattacaaaa tgggtgcaat cgaatgatag aattgcattt acattgtaca accccggcta
    32641 tccttcgcaa cagtgcgatg atgtattgct cgaagaaatg aaccgcattg cgccatcatc
    32701 tgttattgct cgtccaatcc gctttgcaca ttttgctatt acaaaaaaat gcccattaag
    32761 ttgcgaacat tgttttgagt gggacaacct taataaaaaa gaaatactgg attacaatga
    32821 tttgcgaaac atagttttaa aactaaaaca agccggcatt tcgcaattac atattagtgg
    32881 tggcgaacca atgttgcgtg taaacgatat tgtaaagtta tgcgaagaat tttgcaatag
    32941 tatggaattt tggattctta ctgcgggata taatcttaca aaagaaaatg ccacacgact
    33001 taaacaagca ggtgtaacag gcattatagt aagcctcgac cattacaata aaagtatgca
    33061 caacacattc aggcaatcga aagatgcatt tgataatgct ataaatgcaa taaaatatgc
    33121 ccggcaagct gacatggtag tggctgtaac attgtgtgct acaaaatcat ttcttaccaa
    33181 agaaaatctt tatcgctatg cctcatttgc gcactcgctc aatgtttcgt acattcaact
    33241 actggaacca aaggcggtag gccactatgc cggaaaagat gtgacaatca atacggaaca
    33301 aattaaaatt ctcgaagatt tttatttcga aataaataat tcacctacgt atacacacta
    33361 ccctattgtg gtttatttag gatactacca acgtaatgct ggatgcctgg ctgccggaaa
    33421 ccgtgcattg tatattgata ccaatggcga tgtgcttgca tgcacatttt gccatatacc
    33481 atcgggcaat gctttgcgtg atgaacttat ggatgtagta aatagaatgc gtaaaaaagg
    33541 ttgcagcgat tatatcgctt cggttttata aaacacagat attaattgtt tgaatatatt
    33601 tcacaaagcg tggtaaagct taagctgttt tattatttta taaagcgcat ctataaaaaa
    33661 acattttact tcttcacagc ctttattact gtcaatttct catcatccgt tttcaattca
    33721 ataaaataaa ttccgggcca ggcatctaaa ttcaaatcaa ttatgttggt ggcattatat
    33781 tctttaacga atatttcctg accaataata ttttttattg tcacttgaat atttgaatat
    33841 gttttatcca attcgatatt gatgttcccg gtggttgggt ttgggtaaaa tgtaaagtgt
    33901 gcgctttcat ctgcattact actcgtgcgg cagtttacag ttactatttt tcctgaagat
    33961 aattttgaac ataggttagc atttgtaact atcaccctat acgttccagt actaactgcc
    34021 gtataattta ttgaagtttt tcccgctaaa atattgctac cctttttcca ttgatacata
    34081 tagccaatac cgctgttggc agcaagcact acactgtcgc cattacaaaa tgtagttggc
    34141 ccttgtgcgg taatagtagc tgttggcagt ggattaaccg ttaccactgt gcctgtagtt
    34201 gtggttttgg aacatcctgt tactttattc gtcactatca ctttatagct gccaccggtt
    34261 ttggcattgt atgaaggcat tgtatcgcct ggtaaaatat ttacaccttt tttccattga
    34321 tacaaatagt ttgttgccaa aggcgcattg agcaaaacat tgccaccggt acaaaatgtg
    34381 gttggaccag cggcagtaat gttagcagct ggtaatgctt tcacattaac aaataacgat
    34441 gacaattgcc caactccata aatgctattg ccacgcacag taatattacc cgatactgca
    34501 ttggatgcaa agtctacagt aattgaattt gtagtgctgg ttccggttgc cccacccggt
    34561 aaggtccaaa tataactcgt tgcatttgct actggcagta tagaatagat aacgcttttt
    34621 tgccctttac aaacacttgc aggaccggtg atggatggag gacaaccggg cacatgatta
    34681 agcacaacgc tgtaaagtag caatgttata ccatccatac cggcaggcca tgagtttaca
    34741 agcccacaaa ctacagcacc catgcatggc tcataaacca ttcgactttt ataaccccat
    34801 gtagaacctc cgtgcccata agttgtatga ccaaaccata attgtttttc caatccaaaa
    34861 ctataagtat atgcttgagg tgtggtaaca aaagtagtaa gttctaacag tgaggcaggc
    34921 ttcaacaact gaccggtaaa aagtgcgttg taccactttg ccatatcgcc cgaagtagaa
    34981 aacattgctc ctgcacagcc ccctgcagta ttcaagccta cgcgcgaagt atcgttataa
    35041 tctaatgagt tgtaccatct gtgtgcaaga gttccatttg cgttttcctt gacatcgtaa
    35101 aatgagtttg ataaatttaa cggtgacagt atactatcgc gaattatttt cgaaatatga
    35161 attcctgttg cactcttggc aatcatacct gccaaaatat aatttacatt tgagtaactc
    35221 caatttgtgc cgggtgcaaa aagtggtgca cctacccagc tcaatacttc ttggggagta
    35281 aaaacacgag tgggattttt catgatggta tctacccatg gtggaagaaa aataggatcc
    35341 tgcactccac tcgtgtgatt taaaagttgg cgaatagtta tgttaggatt tacattcgca
    35401 taagtaggga tccatttttt caatgagtcg tttaaactta aaatattgct ttcggtaagc
    35461 tttagtatag cagcagctac aaataatttt gtgttacttg caataccgaa ctgcatatta
    35521 gctgtaattg gtgcaccgct atacgacaac cctgctgtgc cttgccatgt gccttgcccc
    35581 ggaagatgta cacttgccga catccctttt acatttgtaa tttgcgacac gtaataattc
    35641 aatgtgtcct gcagcatgtt tgccaatggc tgactgaact gtgcacgaac tcccacactt
    35701 gcgataataa atatcaatgt gaggcatgat tttttaagtg attgattatt catttgacaa
    35761 taattataaa agttttttat tagtacctcc ttgtaaagtc aagtatcctt ttaatatgtg
    35821 ttcgagcaat ttattttgac acgggtagtt tttggtgccg tgtttcttat tgcaataata
    35881 atgctaattt gaagatgagt aatttaagct gccttgttat gtttgataat ttttcaatga
    35941 aaaacaataa aaacggtggt actttggact aaacataatt aaaaaaaaga ataaaaaagc
    36001 aaacaaaatt tctaagcaca cgatttaatt cgaaatatga ctgtcgtttg aaataccatt
    36061 gcaagagaaa aaataattgc tgcctttaac atgtcctatc aaaatgaaac tgataaaata
    36121 atcgagtatt aaaaatacat tgtaagcaaa aatattttta cttttgatac ataaaataat
    36181 aacaaaatga caacaaaaat atcttcttca aatttattgt attctgcaat agtaattaca
    36241 ggaataatgt tttgcacaaa tttgcgttcg caaaattatt gcgatcctac ttttgcaaac
    36301 gggtgttctc aatggcacac taactcaatg acactttata atttaaattg gtttcttggc
    36361 gcaaacagtt gcatgacttc tgattacact gccgacactg ctaccctcac agcaggcaat
    36421 acttatccta tgttagtttc gaatggcgat tggtgcggat gtggcgtttg gatagatttc
    36481 aataatgaca atgcgttcga ttcttccgag aatatgtatc acatgtacat tgcatcgcaa
    36541 actgcacagc attcattcaa ccttgttatt ccggcaaata taagcacagg cgcgtatcgc
    36601 atgagggtga tagcaggttg gggaagcgat acttatacag taagcccaaa tggttatggc
    36661 ccttgcggtg catatcaata tggaaacttc aacgacttta cagtacatgt atcaaatctg
    36721 gcaggaatca ataccaataa tacttcacaa ttttttacag cacaatttat agccgaaacc
    36781 aatgaccttg ttgtaaatat gaatgattta gtacttgaaa aagcaatgct tcagttgtgc
    36841 gatgtaaatg gtaatataat tgagaccaaa gcggtaaccc aaaacaaaga aataatgaac
    36901 cttagcacac aaccaaaggg gttatatttt ttgcgcttta ttagcggtgg aaaaattaat
    36961 accattaaag tattaaaata aaattttggt cactttgttt tggaaaacta tattcctttg
    37021 cgaaaatact gacggggatg ttttacattt tattgttcta aatatgttgt actaaaaacc
    37081 aaaactgcat ctgcagtaac cattatcaaa aataatgtag ataatcaaaa agctgttgct
    37141 aaagatttgt ttttttttca aaacagattt catactatcc ttcaaaattt atttttcgat
    37201 gtgtgccatc gcaatatggt ttattggatg aagcaccgca tcggcaaaat gcagttactt
    37261 tatttttttg tgtggtattg ccttcgctat ctttaacggt aatatttcca tataccagca
    37321 aggggccgtt tggagcaatt tccacaatac tttcggcagt aacttctgca ttattttctt
    37381 tgtcttcgtt cataaaataa gatagcgccc cgctgggaca ttttttaatt tgatcaatga
    37441 ttctttttgt attggcacct tccggtttta tccatggttt ttcggcaggg ttaaaaactt
    37501 cgggcaatcc gttttcacca cgccagcaaa tggcagagtg aatacacact gtaggttgcc
    37561 acactatggt aacctcacca ttggtatact tttttgttat ttctttcatt gtgtaagttt
    37621 gttgttttta gtaattattc ccaacgccaa atatatattt ttgcttttgt tgtttgcaat
    37681 atttgttgct tttacttttg tatttcattt ttacttcgtg atgattattt aattcgattt
    37741 gggtttgttg caaagtgtaa tggggaaaat accaattatc aactcctctt tttttgcaac
    37801 accattgtat ggtaccataa gcaatccgca ttttgatttt ccctactccc actctcattt
    37861 ttgttgcact catttgttaa ctgttacttt gttgaccatt gtatttgttt taaaattttg
    37921 gaaattggga tggattgctt taacaaaagt tattggccta tatcgtaatg atgtcaacag
    37981 cgttgttgtt atttttttta catcacttcg cagatggtgt tgcttgagca acacatttta
    38041 catgagaagt tttttttgct ttttcttgta gttggtataa atcgttatta ttgtgaaata
    38101 aatcatttct tccaatgcag aaaagtttca ataaccaggt aaaaaaaata cgagaaatcg
    38161 ttaatcgttt tgattcggat agcaacagtc tcaagatttc gcttatacgt gatattcagg
    38221 caaacaaaat tttgttcaac catgcgctaa aggattacca tgagtgtctg ctgtttatca
    38281 tgtcgtttcc gcccgatgaa aaaacaagga gtttagcaga aagggaagtg cttcgcattt
    38341 ccggtttgct aaaaaagcaa cctgaaaaaa ttaaagagct gttcgttaat tccggcattc
    38401 cgttaagcag atatcaggcc gcatattcac atgattgtac acgttggttg ctatcgcatc
    38461 ccgattgtac agtggcaata aatgaaatta caaatacatc atttgatcta aacacagcgc
    38521 ttaaggttac catacctgcg cagctgcgaa actataccac tgctgcttac accaacgatg
    38581 ttttgctgga cgaactaatg cctggtaaaa aactgcaact tggatatatc atcaatgaat
    38641 taagccgact cgacaatacg ccttatgtca aagaccatta ttacgaaggc ttgggaattc
    38701 ataccgatat agtacccaaa agcaaattac tttctaaggc ttttaataga atagagattc
    38761 ccgaaacatt ttaccacagc gaaataatta agcgttttga tgtggttgag ttactcaacc
    38821 gcaaactacc cgatgaagct gttctttcat cagcagaaaa ggaaaagctt gttcttataa
    38881 taaaaaactc aatggcaatt acagaaaggg aaacagaccc tgccacttac atgaaagaag
    38941 attcgctgca cctttttcat ctcgaccgag gcgtatcggt agccctgtat ggtctgcctg
    39001 ctgaacgtca acttccaatg gaatcgtaca tcggttatac catgtttaaa aatggttatc
    39061 ctgctgccta tgccggtggc tgggtgtttg gttggcgctc agattttggg ttaaatattt
    39121 atgaccagtt tagaggaggc gagtccgctt ttatggtggc gcaaatactt cgtgtttacc
    39181 gtcagctatt taacgtaaat cattttcagg tagaagcttc gcagttcggc ttagatagtc
    39241 ccgaaggaat tgaaacaggt gtattctggt tttattacaa acttggtttt aggccaaatg
    39301 atttagcatt gcgccaactt gctaaaagtg aacacatcaa aatttcttcg cgtaagggtt
    39361 accgttcttc agaaaaaacc cttttgaaat ttacagagag caatatttcg atgagtctgg
    39421 gaaaacctgt tcaaccggga gtgtatgata taactagtga agtaaagagt ttgattataa
    39481 aaaaataccg tggaaatagt gtgcaggctg agaagatatg cgttgcagat tttctgacga
    39541 aaacaaactg caaggatact tttgctaacg ctgaatttga agtgctaaaa gaagtatcgc
    39601 tgtgggctgc tgcaatgaat attgccgaca acgataaatt aaatctactg ctgcaaatgg
    39661 taaaactaaa acctgtggat gaataccggt atcaggaact actgcgtttg ttctttggcg
    39721 ataattacaa gtaaaaagta tttttctgta agctacagat tcattctgaa agtactccag
    39781 tcaacaccaa tcctaaatgt ttggtaatgc cgcccatcgc taatgctttc accatagtgg
    39841 ctggttgcgg aatgcattgc tcccgaagga taggcaacca tgcgattaaa tcggtaacca
    39901 acacggtcaa tttcctgcca cttgtttcgc tttttcgagt cggtttcaaa tagctgaagt
    39961 aaatcagccc gtttcatttt tagccttcgt gcatcttctg ctgtaggcat atcggttata
    40021 tgtgtttttt gatgcatcca aagcgatgtg ccgcagtcaa atggtaaacc gggtgttagg
    40081 tatacaacca ccgttatatc atcattggga tggtcgtaat gcacaccggg aatttccttt
    40141 ttatttcctt tcgaataacc aaaataaaaa actccatttt catcctttgg gtctgtatcc
    40201 catctgttta tttttatgcc taaaattttt tcgagctttt gtttaacacc cggctgatgg
    40261 tatactttgc ggcttcgaaa tcctgtatag tatcgcggtt cgtaatagtc agcttccata
    40321 gcttcgcgat gaacctgcaa ggggttatca tagaaatcat cctttacaat tataaatcgg
    40381 ggataaacgg ttttagtact cataattgat taatttttta cgaaataaaa ctggtcaaag
    40441 ttaaaatata ctacctatta ggaacaatga ctttattaat gattattttt taagttttag
    40501 tggaaaaagg aagttgttga tacggcagcc aatgccggtt atactgatga tcatttgggc
    40561 cggagaagat ttcatagttg atgctaattt aggattggta aacgaagtta tgttttttag
    40621 ttttttcgca aaaacaaatc agcttgaaca aattaatttt tagttttaca aaatgttatt
    40681 gacgtgcagc tgttgagaaa ttgttttatt gacaacctgt cacgctttat gcagtgaggc
    40741 tgaccttata aaaaattact atgttatgaa aaaagtatat ttattatttt tcctacttca
    40801 cttcacattt aaaatctgtg aagctcaatt tattttggct ggaaccgtat cgggaaaaga
    40861 tacttatttc gatattgttc ctgattctgc gctggaagcg ctaaatgtcc accttagccc
    40921 ttatccaggt gaaacaataa aactggatgt ggatagtgat ggaattcagg attatcaaat
    40981 tgatacatat ggaggtgggg gattgggtgg aggaacaggt gggtgcgtaa tagttccatt
    41041 gggagcgcat gcaagcgtag cctctcatat ggatacgtct gcaggatgct gtcctgcaca
    41101 atacatcgcg caacttgtag acacccttgt aatgggagat acaatttcga atgctctaag
    41161 tttttattca ggtactaatt atttatggtc tacaacttat ggtatggcac aggctcctat
    41221 tatttccgaa tggaatgata taggagaaca ttacatcggc atccgcttaa attacccgca
    41281 ggatactctt tatggatgga ttagggtaga agcaacaaat agcggaattt ttattttgac
    41341 agttaaagat atggcatgca ataaaaatca acatattgga atcaatgaaa tttataattc
    41401 tcagaaatta atcattgccc ccaatccttt ttccgaccaa ataacagttc tttgctctga
    41461 ttgtagtgat gcactatttg ttctttataa cttcgtttca ggaaaaactt tagaaaaaaa
    41521 aattacaaaa tcatttttac taaatacaga acaactagca aaaggaatat atctctatga
    41581 agtgaggaat ataaacggag taattaagaa aggcaaagct gtgaaaaatt gaggcgatat
    41641 atcttcgcta gaaaacaaaa cgtctggtaa caaaaagaaa ggagatgtgt aaaattcgcg
    41701 aaaatatttt atcagaaatt gccgtcataa gtcctcaact gatgattgca tatttcaggc
    41761 aaagccattt gaaaaaagca aattgatttt aagcctttaa caattcgcct gcagacttcg
    41821 ggttgctata cttcgattaa gcagcaagcc gacaacttac acctaccact ttatctcaga
    41881 cagtaactcc aaactggaag tacaagttgc gctcaaccgt tgttgtattt gcttcttcat
    41941 ttctacaaat taaaaactgc acttacaacc ccttgcaaat aacatcatta acgttccaac
    42001 ataatagctt cttattagct aacaaacaat ttcttcgctt gcctccagtt tatcaagtct
    42061 gcactttgta tccgatgcga tttaatttta gatgagaatg atttggattg tttcatggct
    42121 tctaaatgcg ttccgtttcg ataaaactgg ttgatatcgt tttcattttc ccataaagtc
    42181 atagtgtacc agactttaaa attccagttg tttgatactt tatatttttt gcaatttgct
    42241 tgtttcaact ctttaattat ttgcccgtta aacttaaaaa aggcaattaa ctttgaataa
    42301 gaattcaact cgatttttgt tattgatact atcaccgggt attatttgtt ttggttaatt
    42361 ttctatgatc attattttga agtgttgtca gaatataatt ccaaaacctt ttccaattta
    42421 atcaacttga taaataaaga aagacattgt ctgcaaattt aaatatagcg attacttatc
    42481 ctaatatgct ttttgttttt tgtaaatggg ttgagtattc tacccggtag tctttttttc
    42541 agcacttttc atttctttta caaggccttc gggtaagggc cggcatttac aattttttga
    42601 atgctcgata tgccagtgta tgcgttgagc aaatgttgca ttctttggca ttctattttt
    42661 ctcgtgccat tctttattta ttttcatagt tagtatggtt ttctatctaa aaagtaaccc
    42721 aaggtgatac taattgttgg aatagcaaaa tatccaatgc caatggttgt aaaataatcg
    42781 gaatatgttg tttttgtaaa tggaagaaga aataaaatat cgagcacaat gtttatggca
    42841 aaccaactta taccaacaat aatggcattg gttacaaagt cggcatccac aaatttaaaa
    42901 taacgataca gaagatagca accgcttatt acacctacca acataatagt ggatttgaat
    42961 gtggcatagg gcaccatcaa ttcgcccccg ggtttaaaaa acaaaaatga aatagcatat
    43021 ggtataagcc acgacagaaa gccgagtaaa atgtttttgg tgattaattt ttttgtcatc
    43081 gtaaaatgtt ttttaaattt taatcaatag gcaactgcaa attatttttc gttcagatat
    43141 ttttttcggc ctcggcaata ttttctattc ccttaattaa agcatcggga ttgaatgaaa
    43201 tactatcgat accattctta accagaaact gtgcatactc gggatagtcg cttggtgcct
    43261 gtccgcaaag gcctgtatgt gttccggttt tcttggcttt agtcagtgtc atttctatca
    43321 gttgcattac agccgcatcg aaaggactaa atatttcgct gagcaattcc gaatcgcgat
    43381 cggcaccaag agtgagttgt gttaaatcgt tggagccaat agagaagcca tcaaagtgtt
    43441 ttgcaaattc ttctgcaagc aatgcattac taggaatttc tatcattaca tataattcga
    43501 ggccgtttat gtttcgttcg agcccgcaac ctttcatcac ttcaattacc tgttgtgctt
    43561 cttttatggt gcggcaaaat ggtatcatca attttatatt tgtaaggccc atatcatcgc
    43621 gtactatttt catggcttta cattccattt caaatgcatc tttatacaac ggatgataat
    43681 accgcgaagc accacgaaaa cctatcatgg gattttcttc accgggctca aattgtttac
    43741 caccaagaag gttcgcatat tcattagatt taaaatcgct catgcgcaca attacttctt
    43801 tgggataaaa tgccaccgcc atttttgctg tagcctcggc caacttctga acaaaatatt
    43861 cttccttatt tttaaattgt tttgttagcg cttcgatttt ttctttcgct tcggcatcat
    43921 ttattgtgtc gaaatgtttc aatgccatag gatgaattcc aattgtattg ctgataataa
    43981 attccatgcg cataagccct actccatcgt ttggcaattg tgaaaatgta aatgcttcat
    44041 cggggtgtgc cagaatgagc atcgctttgg ttttggtttt ttgtatagaa gaaatatcaa
    44101 tttctttctc gtcccatttc aaaagaccat cgtatacagt ccctgtatta ccggcagctg
    44161 ttgatacagt gattgtttgt ccatcgacaa tattttttgt gccggtgccg gtgccaacca
    44221 cagctatagc gcccaattcg cgtgcaacaa tagcggcatg cgaagtgcgg ccgccctgat
    44281 cggtaataat ggctgtagct tttttcatca ccggatccca atcgggatcg gtacgttcgg
    44341 ttacaagtat ttcgcctttg ttgagttttg caatttcggt gggcgagcta agtattcgtg
    44401 ctttgccggc cacaatttta tttcccaatc cggcaccgga gcatagtttt tttcctttat
    44461 cgagcaaagt gtattgctta aaaacgtttt gctttttttt gctgtgaacg gtttcgggtc
    44521 gcgcctgtac aataaatatt tttccatcct cgccatcctt tgcccattca atatccatgt
    44581 ggcatttata atgttcttga ataatgcatg cccattttgc caattgaata atttcggaat
    44641 cgttcaatac aaattgattt cttttttctt cggtggtatc aatattaata attgcttcct
    44701 cgggttttac aatatcgttt acactcacac ccgaatatac cattgtcttt attttgctgc
    44761 ccagtttgtg cgcaataatt ggttgcttca atttatgcag gaatggtttg taaactaaaa
    44821 actcatcggt attaatagtg ccctgcacaa cattttcgcc aagaccccag gcgcccgaaa
    44881 taagcacaac atcttcgaat ccggagtcgg ggtcgatggt aaacattaca cccgaactgg
    44941 caatatcgga gcgtatcatt gtttgcacgc caatggataa agccactttc atgtgatcga
    45001 aaccattatc ggtgcggtat tttattgctc tatcggtaaa taacgatgcg taacaacggt
    45061 gacaggcttt tatcagattt tcatcgccac gcacattgag gtaagtttcc tgttgtccgg
    45121 caaagcttgc tgtaggaagg tcttcggcag tggcactgct gcgcacggca aaagatttat
    45181 tatttccggt actgttattt agtgaagtgt atgcagcaat aatttcttct ttcaatgcaa
    45241 caggaaaagt gctgtcgaga attaatttgc gagcctttgc acctatttcg gcaaggttat
    45301 cgaaggtggt aatatcaagt aaggaaagaa cctgtgttag ctgaggaact aaattatttg
    45361 ttttcaaaaa actccaatac caggccgatg taactgcaaa gccctcgggc acggccactc
    45421 cttttttaga aagcgacaca tacatttcac caagcgaagc atttttgcca cccaccagtg
    45481 gaatatcttt tataccaatt tcagagaaat gtttaatcga attcatagcc tataaaattt
    45541 aaaataatta tgctttggtg taaaaatgtg cataatggac aaacagctac atgataataa
    45601 tcaattgaag aaatgatatt ggtcatgaat tggcttgctt aatgaatttt aaagttgata
    45661 catgtgcgaa aagaaaaatt tttacagctg tattctaatt aaaaaaattg tgccgcaaaa
    45721 atttttgagc agccataatg aaaaggacta ttttatttct cgatgcgaat atcatactta
    45781 tccaaaagcc cggcaatggc tgccagtgtg aactttgcct tcttcaattt gttcagttcg
    45841 ggattgatgg aatagttata ggctgtacga aaatcggctt tttctaaaat ggtattttca
    45901 aattttgcat ttgctaaatc gcaatgatcg aagatagatt ccgataaatc gcaatcggta
    45961 aaatctactt catgcaagcg gcaattgatg aaggaagttt tttttatttt ccgtttgtaa
    46021 aacgatgagt gcgtaagatt acaattatcg aaagaggcag tgaggccgaa ttcgttgcaa
    46081 ttataaaata gcaacccgag cattttgcat gcactaaatt tcacatcgcg aaaggcagtt
    46141 ttcgaaattt ttgctaaact taaattgcaa tctaaaaatt gacattctaa aaaaaccatt
    46201 tcactcaact cggaatttgc aaaatcacaa tttttaaaaa cacaatattc aaattctgtt
    46261 tttgcaattg gttgtttagt aaaatctatt ttatcgaaaa ttttattttc tacaatttcc
    46321 attttctcaa ttctttaagg aatgaatata ggtggcaaaa attatttcgt tccgcctttg
    46381 gtgtttaaca attttgcaca aagtaaaata ataacacacg gaaagatgat aaatttaatt
    46441 tcagaaatca atacagaaag tccccatttg gtaaaaaaat tggcagcacc tattggcgaa
    46501 acatccacag gtgtaaacca tgggcaaaaa taacgctggt tggaaaatgg cgcaaacagg
    46561 gcaacaccga gccctccgtt tgtcagcata tcgagcaatg ggtgcgatac agtagcaaga
    46621 aataacgata aaaaaataat gcggccttgt gaagttgtaa aagactggct ataaaagaaa
    46681 attactttta ccaccatagc caatattgca gcaaaaaaaa tggaatgtgt aaaaccacga
    46741 tgcccaaaca tactattgta tgaaacacca aattgaaaac cgacaacatc ggcatcagga
    46801 atcatggcgc aaattataac aagcgtataa agtttttggg tagttacctt agtaaaaaaa
    46861 gttttgctta atgtataagc agcaaatgca tgtgtaaata ttgtgggcat tttggtttgt
    46921 gtaaaattat atcatgttag tttttaaaaa taaaatgtgt gtggtaaatg taaataattt
    46981 ttgatgcatc ggctgagtta ttgcattgct gataattcat tagaaaacag aactgcactt
    47041 tgtaccattg tttcattcaa aaaaaaacac cgtatcattt ttacatgaaa cggtgttcga
    47101 aaaaaaaact aaatcaaaat atttctttat gacgcgctct tctttggctt tttcggattg
    47161 ccattatttg cttttgtaat tacggctttc aactgatttt gctctttgtc gaaagtaaga
    47221 tcgatagaat cgccttcggc caattccgat ttaataattt cttcggcaag caaatcttca
    47281 acataacgct gtattgcgcg ctttaatggt cgggcaccaa agttactatc gaagccttta
    47341 tcaccaataa aatcttttgc ttcagcatcg agcgacaatt tatatcccaa gccttctaca
    47401 cgtttatata acgtagcaag ttcgatatca ataattttat gaatatcggc cttatccagt
    47461 tgattgaata ttaccacatc atcaacacgg ttcaaaaact caggtgcaaa ggccttcttc
    47521 aatgcatttt caataacact tttggtattt tcatctaccg aatttaattt tgctgctgta
    47581 gaaaaaccta cgcccatacc aaaatcctta agctgacgcg aaccaatgtt cgaagtcatg
    47641 ataatgatgg catttttgaa atctactttg cgtccaagac tatcggtaag ctgaccatcg
    47701 tcaagcacct gtagtaacaa attgaaaaca tccggatgag ctttttcaat ttcatcgagc
    47761 aatataacag cgtaaggttt gcgtcttact ttttcggtga gctgtcctcc ttcttcatat
    47821 ccaacatatc ccggaggtgc acctataagg cgactcacgg caaatttttc catgtactcg
    47881 ctcatgtcaa tgcggataag cgcatcatcg ctatcgaata aataacgggc aagcactttg
    47941 gctaattctg ttttaccaac acctgtaggg ccaagaaaaa taaatgagcc tatagggcgg
    48001 ttaggatcct tcaatccggc acggttgcgc tgtatggcct ttaccacttt tttaatggct
    48061 tcgtcctgcc caactacttt tccttgcaac tctgttggca tttgaattaa gcgtgctgtt
    48121 tcggcttgtg caatgcgatg cacaggtaca ccactcatca tggctactac ttcggccacg
    48181 ttttcttcgt taactgtaaa gcgttgcaag cgggtatctt cttcccattt caattttgct
    48241 ttttccagtt catcaatgta ttgcttttcg gtatcgcgca ggcgtgcagc ctcttcgtac
    48301 cgctggctgc gtactacctt acttttttca ttctttattt cctgaatttt attttcaaga
    48361 tcgagaattt cttcgggcac atgtatatta tttaagtgaa cacgcgaccc agcttcgtcc
    48421 aatgcatcga tggctttatc gggtaaaaat ctatcggtga tgtaacggga agtaagcgta
    48481 acacaagcct ttatcgcctc gggtgtatag tttacatggt ggtgctcttc gtacttatct
    48541 tttatattgg taagaatttc aactgtttct tcggccgatg taggatcgac aattactttt
    48601 tgaaagcgtc tgtcgagtgc gccatctttt tctatgtact gacggtattc atcgagtgta
    48661 gttgccccaa tgcattgtat ttcgccacgt gccaaagcag gcttaaacat gttgcttgca
    48721 tctaatgaac ctgatgcacc tccggcacca atgatggtgt gtatttcatc aataaataaa
    48781 attacatcgg ggcttttttc aagctcattc attacggctt tcatacgctc ttcaaactga
    48841 ccacggtatt ttgtgccggc caccaacgat gcaaggtcga gcgtaacaat gcgtttgtta
    48901 aacagcacac gcgatacttt gcgttgcaca atgcgcagtg ctaatccttc agcaatggcc
    48961 gattttccta ctcccggttc gccaattaaa attggattat tcttttttct gcgcgataaa
    49021 atttgtgata cacgttgtat ttctttttcg cggccaacta tagcatccag ttttccttct
    49081 tcggccgact tggtaaggtc gcgaccaaag ttatcgagta caggagtttt tgattttgaa
    49141 tcacccgact tttttggtgt tgtcatgccc ggtccgtctt catcgcgtgc ataaggatca
    49201 tcttcaccct gctgaggtgt ttcgcttttg aaaccattgc tgcggcttac ttccaactct
    49261 tcgcgtacaa catcgtatgt aacaccgtac tgattgagta actgtgttgc aatagtgtct
    49321 tcgtctttca aaattgaaag taataaatgt tcggttccta ttacttcgct tttaaaaatc
    49381 tttgcttcga ggtaagtaat cttcaacgct ttttcggctt gctttgttaa tggaatattt
    49441 ttcaggttcg acacattgcc cgaacttccc tttacagagt tttcgacaga cctgcgcaaa
    49501 tccaccaaat ttattccgag gctttttaaa aatcttatgg ccattccttc gccttcgcga
    49561 atgagaccca acaataaatg ttctgtacct atgtagtcat gacctaagcg caaagcctct
    49621 tcgcgactgt atgtgattac atcttttact ctcggtgaaa atttagcttc catatctcac
    49681 aatttttatt tcttttttat tcttcgttat ctaccacaaa caacatgcaa taataacttt
    49741 ggtttcgaaa tgtaaatact tttttacaat aattatgtaa aatgtttttt actttttatt
    49801 aattaagcat ttattgttcc aatgtttaat tgatgacaaa cttacttgat tttaaaatgc
    49861 aaaaatttta ttccatgaag ttcgtattaa tactttcaac agttatatca acaatagaaa
    49921 atcgaaatag atatttgcac agaaaaaata aagacaaatg aacaaaccaa ccttagtgct
    49981 tggcgcaagc gaaaaccctg aaagatatag caataaagcc ataaagcgac ttgtatcggc
    50041 aggtcataac gtttatgcca ttggtgcgcg caccggcaaa gttgatgata cttttatcca
    50101 aaaagataaa cagccgctaa ttggcataca cactgtgaca atatatctta acgaacaaaa
    50161 tcaaaagcag tattatgact atgtgttgca attaaatcct gaaagggtga tttttaatcc
    50221 cggtgcagaa aatgcagaat tcgaaaaaat gctggaagag aaaaatatcg aaacgattga
    50281 agcctgtact ttggtaatgc ttagcattgg tgctttctag ccaacctaat gaaaaaacac
    50341 tatcgcaact ttatggcatc gccctccttt agaaccgggt ttgcgggcaa cttattaaat
    50401 ttcatcaatc gttttaatcg tacaccgtac aactgtgata tagaacgtaa tgtatcgccc
    50461 tttttcacct ggtgaacttc ttcatcggca tggcggtgtt tatatgcgag ataaataatt
    50521 tcgtcaacct tcaatatatc gttggcttga gcatcattgt atcgcaacaa gtcgcgcaat
    50581 tccatttgat tttgctttgc cagtttgtac cacgaatcgc cacgctgcac cttagttgct
    50641 tttatattgt tgtgatttat tattagttga ttggtgattg ggttattagt tattgggtta
    50701 ttgattgatt ggttattggt tattgggtta ttggttattg gatcaatagc aataggttgt
    50761 ccggcaatca tagtggtacc atcataaata tacaattggt aggtttctat taacttaatt
    50821 aaacgctcgg catagttagg attggtagcg taaccggcag ttttaagtcc tttggcccag
    50881 cccttataat cagtaacatc taaatcgaat agcgttttgt acctgtcgcg cgagcgcaaa
    50941 aaatcggaat ggtcatcgaa agatgcttgt gcatgaggat attttctaaa acactcgttg
    51001 gccgtttcat catcctgaat aaaagtttcg cccttccatt ctttgtgaca tttaatacca
    51061 aaatgattgt tggcaagttt agccaaatcg ctgttgccac aatccgactc gaacaagccc
    51121 tgtgcaagag tgatgctggc cggcacgcca tattttttca tatcggctat agcggcatct
    51181 ttatattttt cgatataggc tgttggcgaa catttaagta tagaatcctg tgcaagcgaa
    51241 ttttgaacat tggcacaaat aactacaaag gcacagctaa ttaaataatt tttcataaaa
    51301 aagaatagat gatgattaac gtagaataga aaacaataat ttcctttgct cacaaaatta
    51361 ttttaatgcc gccaatcaaa gttgcactgc agcatgactt agtaacattg cattgttaat
    51421 agttgtgtgg caaaaaaatg gcctcgtttg tattacacag cttt
//
LOCUS       DAORQA010000008        39582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_211_length_39582_cov_26.295241, whole genome shotgun sequence.
ACCESSION   DAORQA010000008 DAORQA010000000
VERSION     DAORQA010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 39582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39582
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211_length_39582_cov_26.295241"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PK736_01560"
     CDS             complement(<1..472)
                     /locus_tag="PK736_01560"
                     /inference="COORDINATES: protein motif:HMM:NF017634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HRC32110.1"
                     /translation="MEEQKNIYDYAIVGAGAAGLHLALAMIDDNYFATKKILIVEKES
                     KNINDRTWCFWENENGLWDKIITKSWSTGFFITKEKSIKLDMKNYHYKMLRGIDFYNH
                     AKSKISSTENISWINDDVKSVQEESGNVSINCSDKKYLARHIFDSRVPQDYFAPD"
     assembly_gap    544..553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            913..1308
                     /locus_tag="PK736_01565"
     CDS             913..1308
                     /locus_tag="PK736_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC32111.1"
                     /translation="MKQHLAKIALVVKDYDEAIAYYTQKLNFELKEDTKLSDTKRWVV
                     IVPPGSEGCEILLAQAANDRQLAAVGNQTGGRVFLFLYTDNFYRDYNNMVTNKIKFVR
                     PPSQEPHGTVAVFEDLYGNLWDFIEPEGK"
     gene            complement(1441..1590)
                     /locus_tag="PK736_01570"
                     /pseudo
     CDS             complement(1441..1590)
                     /locus_tag="PK736_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
     gene            2084..3334
                     /locus_tag="PK736_01575"
     CDS             2084..3334
                     /locus_tag="PK736_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32112.1"
                     /translation="MTPILFCFTLFYRHNNAAIKKMWLLTAIIVLMFFIYFLSKNSIY
                     NVLILFASIGFNAALITIICLVIYKFRNIRTLFPFVVTALFIIILILSFISAVLSGNS
                     NLLAAYFYPVYDFSKAIGMENYSTTVFYFINILILICGFALGFMLLNYSFIKKANNNT
                     IMLIDFVWLSHILFTLSLPVADTSLYIYMVAVFAVYKLVQIISFKIFIPKVEKGINKT
                     LLVLRVFSLKHESEKLFDMIRSYWLLNGPITLIAGHDLTASVVDIEDMLYLLTGRIKN
                     RFNTTPLLIEKNKSKIDNIADIDGRYRVNEMYCTNTTWKRVLKNLIQTSNKVMMDIRT
                     FTELNKGCAFEIKEIIYRVPLNNILFIANGKTDLKLAKSIMEEAWNVMPQTSPNINES
                     GQMIHVFMYETQDDLGQLINTLKK"
     gene            3442..4533
                     /locus_tag="PK736_01580"
     CDS             3442..4533
                     /locus_tag="PK736_01580"
                     /inference="COORDINATES: protein motif:HMM:NF021640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HRC32113.1"
                     /translation="MATTDREKIDNAFERLIEQYNAALTATTLQDAATAENAFRQATV
                     IYAGIEKIDFPDEKSFMSWVVAFIELLSNYSKANFLFMEERFKKSKEEFDKTLLVIAS
                     LHEKQKDINQEFVTEFELGKLYGFINFFTSYFGHLTNVMINLALLSQQMLEGKYINEV
                     LLNQNAAKEIRGFDYKKYTTDDDDFNSEIIKIVGLLGRIADVYDKKAEKAEEKIKTIE
                     FLKPIDKNVFLVHGHEEGNLRELKDLLKDNFNITPIILRDEQDGGKTIIEKLEDYGRK
                     CAFAFVLITPDDVVENKKTKAFQARPNVLFELGWFCGRFGRSKVRMLRKNETPLPSDL
                     HGLIAYEFNEKVSEVFLNIKKDLEAAGII"
     gene            complement(4588..5454)
                     /locus_tag="PK736_01585"
     CDS             complement(4588..5454)
                     /locus_tag="PK736_01585"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRC32114.1"
                     /translation="MKEEIKMTTSRKIKNIHTAPQVMMGNIALRQPFPLHGFAQFSPF
                     ILLHHFDFDIEPGNNSFDVPPHPHRGFCPVTFMYEGRVSHNDSLGNSSVIGDNEVQWI
                     NAARGIIHSETTDKDFVETGGRFQGIQLWINLPADEKMKTPSYMPITKNEIVLIEKDG
                     VQFRLISGKYLDKKGPAPSNVFTAMMRMQLNSKFEIEFAASYNVAIYILEGDIEINNL
                     EKATQHQLVTFENTDGAITLEALSPVKLLLMAGEPIHEPLVTHGPFVMNTQTEILEAM
                     RDYQQGKMGYLY"
     gene            5618..>6195
                     /locus_tag="PK736_01590"
     CDS             5618..>6195
                     /locus_tag="PK736_01590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRC32115.1"
                     /translation="MKISLKHILILGIITRVMFLVFGAPLYYGRTPFYMHDDSIGWMD
                     SIVNLINTGTYTANAAWANGVFYRPPGYSFFLGFCYLITGMSVERGFLCAVILQSALD
                     ILNIFFCYKIVFQLTQNKNTSLAGAFIYALYPFTLVWDGAATAEILSVTLMFASFYFY
                     TNPTQKYRLALSALFLGFASLTRPQCIVLFPC"
     assembly_gap    6196..6231
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6232..7101
                     /locus_tag="PK736_01595"
     CDS             <6232..7101
                     /locus_tag="PK736_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32116.1"
                     /translation="PCIIAVEFIFNIKNPKKYLKNILQLLLFFSLSYGLWPARNYINQ
                     NKLIFIQDANIGNVWSPDWFAFMRYIFAVQTDIEPQASQLMNGEKVTWNEQSYHYSGD
                     SANLASFTNMLDTCGTGVSYFRLHKNLIDTPVASNQNCDAAIAKLSEELVANQKKYNA
                     FNYYINVPFSNLKKSLFKSGLYGKQSNSVKIIASLLFGIRTVGILAGLFCCIWLLLFT
                     SIRKEFLTITIMYFAAWYAYMCWLFRNIEVRYFLNADVLLLIPLAIVIGMLIDKYYLH
                     RKTDVISIEDKNS"
     gene            7124..8095
                     /locus_tag="PK736_01600"
     CDS             7124..8095
                     /locus_tag="PK736_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC32117.1"
                     /translation="MNDILIIDPDTKAQLTKSSEGLLQNGLVKFPFKNGAYRLVSDSN
                     YTENFGKEWNTFQTTQIDKHTGNNVSKVRLFAQTGWKENDFAGENILEVGSGAGRFTQ
                     ILIDYTKANIFTVDYSNAVEANFRNNGPHERLNIFQGSIYDLPFAPKQFDKVICLGVL
                     QHTPDFKNSVQCLCNMVKPGGQLVIDFYPIKGWYSKINAKYMLRPFVKNMDHQRMMKL
                     IDKNVDWMISAHMFFKKIGVGKIVNRFIPVCDISTTVPKGLSKAQLREWVVLDTFDMM
                     SPAFDNPQSLATVSQWLKEFGMEQIDASMVKFAEVFEAATIRCTQKQ"
     gene            8128..8991
                     /locus_tag="PK736_01605"
     CDS             8128..8991
                     /locus_tag="PK736_01605"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRC32118.1"
                     /translation="MKKIILNSIRKIFCYAPFRNILRRLTNGKSPHSIIGRIAPPNFL
                     FAKNSIAKFNAEGFVYELDLSDYIGHYLYFGFEDDGLSDLYNRVSDGAVIFDIGANIG
                     STAMHFATCANNTTVYGFEPDAHNFKIASKNIELNNLTNIKLYNFGLGDKKMQSKLFI
                     KDDNNRGMNRILNDEKSELSYSLIDVRTLDDLVEEIKPTKIDVIKIDVEGFEMKVLTG
                     AKNTLIKYKPKLFLETNNSLLANHQSSISDVVQFLITLNYKLSIIPGNKPLTDDDIKG
                     ANHFDLFAEQN"
     gene            8993..9910
                     /locus_tag="PK736_01610"
     CDS             8993..9910
                     /locus_tag="PK736_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRC32119.1"
                     /translation="MKVKNFLFHRVNDNPDPFWPAMLTSQFKSILSQIKKSYHVISTD
                     ELLHLKKHHGGKTLATISFDDGYKDNIEYAAPILQHYKMPATFYVVTNCIDNNVPTWT
                     YDIDYRTYFTKNTIDFSSCNLPESLLHKQRDKKTSTAQFTGALKAYLKKIDFNKVLEI
                     VKTIEEQTQDIDMPKLMMHWDDIMQLYKSNFIIGSHSHTHPMLNNITNENIVRAELVI
                     SKSRIEEKINSHISSIAFPVGGYNETVKTLSAEEGYKYAMAVEQKSHGENIDLFAIPR
                     IELYNEPRWKTFLRMNEVMQRTKKIVRHG"
     gene            9903..10709
                     /locus_tag="PK736_01615"
     CDS             9903..10709
                     /locus_tag="PK736_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRC32120.1"
                     /translation="MDKPVILMLCNNAHNQIALANKMHTSLGIDHIIVENKITNRKLS
                     LQNIWNKLRYKTIDDAWFAMLSFYKNKFPAFPATNVSFTIDINSAETESIIKTLKPDL
                     ILVSGTRIIKNNIIGINPPLGIMNMHTGLSPFVNGGPNCTNWCIALNKFHLIGNTIMW
                     LDAGIDSGNIITTSQVDFTGNETLTDLQIKVMEHAHQLYIVAAKAVIANKNSVNNIPQ
                     KSISTGNTYYTKMWTSKQKKDLLQNFKHFANVIKSESYLQNKNKIKTIPL"
     gene            10713..12575
                     /gene="asnB"
                     /locus_tag="PK736_01620"
     CDS             10713..12575
                     /gene="asnB"
                     /locus_tag="PK736_01620"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRC32121.1"
                     /translation="MCGIAGFYDNKNTGDEALLRSMTVSLAHRGPDAEGFFYNGTIGL
                     GHRRLSIIDLDIRSGQPFYSQNKRYIIIFNGEIYNYIELREQLKQNHSIAFNTTSDTE
                     VLIECWNIYGVETLQKLNGMFAFAIYDTEKKEITIARDHCGIKPLYYTVQNEAMFFAS
                     ELKALKKVNTLNFEIDTTAIAAYLHAGCVPAPLSIYRHIKKLLPGHYVTYGKNGLHTF
                     QYYSLTKNIASHYLTDYKSSKQHLDSLLNTSVNIQMRSDVPFGIFLSGGVDSSLVTAL
                     AVKNSQHTINTFSIGFAESSHDESKFAQQVASHLKTNHHPFEVHYKNAMELASQITLI
                     YDEPFADPSAIPTYMVSELARKHVTIALAGDGGDELFFGYGSHQWAARLSKPAMKYSR
                     HAIAKTMNMMSSRYKRIARLLHEPALSNFHSHIFSQEQYYFSEKEISQFVKTTKSFEL
                     ILEAKDLHFEKKFNRKLSSIEKQALLDFYLYLPDDLLTKTDRVSMKHSLEVRVPLLDH
                     NIVDYAMNMPTDWKVKNGVAKFILKDILHDYLPSQYFNRPKQGFSIPLARWLKNDLHF
                     LIDDNLNETIINKFGVLDSKYVTGLVKKFNDGNHDFLFQRLWNLIILQQWLRNN"
     gene            12557..13429
                     /locus_tag="PK736_01625"
     CDS             12557..13429
                     /locus_tag="PK736_01625"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HRC32122.1"
                     /translation="MATQQLNKAAFSIDLEDWYQGIEKPFADWPSYETRIEKGFYAIF
                     NLLQKHNTKATWFTLGWVAEKYPHLIKELHAAGHELGSHTYSHEKVYNQTRDEFREEI
                     KKTKQLIENITGKEIVAHRSPFFSITNKSLWALDILKEEGYTIDCSISPIKTWRYGIS
                     TCPDKIFSIKENGLIEFPVSRFKFLKKNWAIGGAYFRIFPYNFTAMGMRNRDKENLPN
                     MFYIHPWEYDANHPKVPMEWKAKTTHYFNLSKTISNTDKLLNEFKFDTVSNIIEQYAK
                     HNTIEQISIDVLQD"
     gene            13377..14471
                     /locus_tag="PK736_01630"
     CDS             13377..14471
                     /locus_tag="PK736_01630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC32123.1"
                     /translation="MQSTIPSNKSALTFCKIDNRADNSDLIKYVAQVLGETNSHKATI
                     MTNEFWKWQHLLLPGGEACVYKSMYENKIAGYYHVPVYPSLINGEAAKFAMIQDVAVN
                     SEIRGQGVFRQLATFANSDLDIMDFDAIYTFPNTKSKHTFIKYNGFTHLHTFTAYMLP
                     VNVGLIAKAKTKIPFLFETLFAPINWMLGPRKKSDYSFTEINKFDIDVVKLYNEYCGA
                     YKFYAHRTAEYLNWRFLQRPSSKYFCFAAKTNNALKAIAFFKIDVMMDVPVLLLIDYA
                     HNDERAFSQLVNYVSANALKLCGSKIAFTFTSVCDKNFDAQKPQGFIKVPEKVNPRLL
                     NLLIRPLKYKQQDIFDANNWFVTLADWDVF"
     gene            14504..15691
                     /locus_tag="PK736_01635"
     CDS             14504..15691
                     /locus_tag="PK736_01635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC32124.1"
                     /translation="MPKILRIINRLNLGGPTYNVGYLTKYLAPEFETILCSGVKDDTE
                     ESSEFIISELGITPHYISTMRRELNIVDDYTAYKEISELIKKHKPDIVHTHMAKPGAV
                     GRLAAIHNNVPVVLHTFHGHYFHSYFSPLKTKLFKSIEQYLARKSAGIVTISKLQQQE
                     LSVQFKIAPPEKTFLVPLGFDLSRFSENMTEKRKSFRTKYGIADDEIAIGIIGRLVPI
                     KNHKLFLRAISEVLKKTNKKVRAVIIGDGENKKEVIDLCFRYKLAFGTDNFETENHPV
                     IFTSWFLNIDEALAGLDIVALTSLNEGTPVSLIEAQAASKPIVSTDVGGVRDVVLENV
                     TALLSPSENAEILTKNILALVEDEHLRTKFSQAGPSHVNKNFHYTLLVENMRSLYHSL
                     LNK"
     gene            15688..16911
                     /locus_tag="PK736_01640"
     CDS             15688..16911
                     /locus_tag="PK736_01640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC32125.1"
                     /translation="MSKTILYLSYDGMTDQLGQSQVLPYLCGLSKKGYVIHLVSCEKK
                     ERFEVHKNLIQNIVDENNIMWYPINYHKSPPILSTLFDIRQLIKKAEELHAIYKFDVV
                     HCRSYITSFAGQWMKKKYGTKFIFDMRGFYADERVDGNIWKQSNPIFKFVYNYFKNKE
                     KQFLQQADQIVSLTHNAKKIIDEWKLNGNNSLPINVIPCCADLSHFDYNNFTADFMDK
                     KKQSMGYISDDKIMVYLGAIGTWYMLPQMLDCYKIFRKNNQKIKLLFITNEPGEIILK
                     QVTQKSIDINDVKIISATRNEVPPLLSICQYSIFFITPTFSKQASSPTKQGEIMGMGI
                     PIICNSGVGDTDYIIEQTHSGVLVPQFTDLEYQKAYERIENTSFEKAKTRQGANQFYS
                     LQKGVEAYENIYKKL"
     gene            complement(17346..18203)
                     /gene="ygiD"
                     /locus_tag="PK736_01645"
     CDS             complement(17346..18203)
                     /gene="ygiD"
                     /locus_tag="PK736_01645"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HRC32126.1"
                     /translation="MLPIAGSITSLESLAKAAGSFAKSESMPVLFVGHGSPMNAIEDN
                     RFSREMKTIGKRMGKPTAILMVSAHWETKGSFVTIQERPQTIHDFGGFPQALFEAQYP
                     APGSTWLAGETQKAVASNQIAFDGKWGFDHGCWSVAMNMFPLADVPIVQLSLDYTQTA
                     SQHYALSQQLKTLRNKGVLIIGSGNMIHNLGMLSFKGNSFDEPYGYDWAIELNSKLKQ
                     DIENKNHQSLINYTSFGKAASLAIPTPEHYLPMLYTLAMQNEKDSLEFFNDSVVAGSI
                     SMTSFFFSS"
     gene            18391..19386
                     /gene="pfkA"
                     /locus_tag="PK736_01650"
     CDS             18391..19386
                     /gene="pfkA"
                     /locus_tag="PK736_01650"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRC32127.1"
                     /translation="MVKDQNKPIKNIGVFTSGGDGPGMNACIRAVVRTAIYHNVQVTG
                     IMRGYEGMIDNEFIPMDARSVSNIIHRGGTILKTARCPRFFEYEGRKKAYENLMSNNI
                     DGVVAIGGDGTFTGAEIFFEEFGVPMIGTPGTIDNDLFGTDYTIGYDTAVNTAIEAID
                     KIRDTAAAHDRLFFVEVMGRDSGCIALAAGIGGGAEYILLPERDTTLDQLIEVLERGA
                     KNKKTSSIVVVAEGEHGGGAYKIAEKVKEKFKHYDTKVTVLGHVQRGGSPTCFDRVLA
                     SRLGVAAVEALLGGESRKMAGLINNKVVLTPFKDATSQDFQLDKSLLKIAEILAV"
     gene            19411..21021
                     /locus_tag="PK736_01655"
     CDS             19411..21021
                     /locus_tag="PK736_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HRC32128.1"
                     /translation="MNFDFPTIDQIPEEYKITTPVEQKVYLTNGELIEWKGETADVFS
                     PIYIKTQPSLYTQFRIGSQPVMNKEATLAVLDNALKAYDNGRGEWATMRVKQRIEMVS
                     HFAKRMKEKRDEVVKLLMWEIGKSLPDSQKEFDRTIDYIQETIDALKNLDRDSSRIQS
                     NGGIYAQIRRGPLGIVLCMGPYNYPLNETFCTLIPALIMGNVVIFKPSKYGVLLHSPL
                     LQIFCDCFPKGVINIIYGKGSETAGSLMETGKIDVLAFIGTSRVANILKKQHPQPNRL
                     RGALGLEAKNPAIILPDADIKITVDELIAGSLSFNGQRCTALKIIFCHHSIQSQLVDA
                     YKAKLATLVAGMPWDKNAMLTPLPEKGKTAYLQELIDDAIAKGASIINENGGEIFCSY
                     MHPAVLINVNSTMRIYHEEQFGPVVPIVTFSNINEPINYMVQSNYGQQVSVFGSNSVE
                     IAKLIDPLANQVCRVNINSQCQRGPDVYPFNGRKDSAEGTLSVNDALRVFSIRTLVAA
                     KNSDLNKHIVSEILEQRTSNFLNTDFIL"
     gene            21065..21922
                     /locus_tag="PK736_01660"
     CDS             21065..21922
                     /locus_tag="PK736_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HRC32129.1"
                     /translation="MILVADSGSTKADWKLAYRGKEMVTFNSIGLNPFFHDSNSVAAT
                     MQVIPQVQQYANEVHRIEFFGAGCSHPARNKIISDGLQIVFPNAAINIHHDLDAAAYA
                     TCNDKPGIACILGTGSNSCYFDGTKVHELNHGLGYILGDEGSGSFFGRKLLAYYLYKV
                     MPDDLRTQFFETYKVTKESIMQQVYYQPNANVYLASFSRFLSVNTNHTFITNLVSRGL
                     GEFMDVNVCNIENYKNVPVHFIGSIAYHFQNILKQEAAKRSITVGNIIAKPIESLTQY
                     FLEKENLQK"
     gene            22293..22829
                     /gene="bstA"
                     /locus_tag="PK736_01665"
     CDS             22293..22829
                     /gene="bstA"
                     /locus_tag="PK736_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018780520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HRC32130.1"
                     /translation="MELDKLKYPIGKFQYTESATAEQIKMWISQIEALPEKLIATVHQ
                     LTEAQLDTPYRPQGWTVRQVIHHIADSHANACIRFPLALTEDNPVIKPYQEELWAELE
                     FQKKLPISNSLQLIKLLHQRWVVLLNTMTEKDFARTYKHPQYGKEYSLTEALGLYAWH
                     SNHHLAHITNLIDRNFDL"
     gene            complement(22804..23400)
                     /locus_tag="PK736_01670"
     CDS             complement(22804..23400)
                     /locus_tag="PK736_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC32131.1"
                     /translation="MVTINIVIIESDSVVHKYYQSIFYKVQDIVIVGCYANLKAFVSN
                     IDSHKNDLILLDVHSTEGYAETVILKLVKQHPEIRMVILSAWIQYDLVYDCIKAGAIG
                     HIGKFVKAQSFIRTIREIYKGESRVSLPLAQLINENILMQRIGKVSIRNMQMLALFSG
                     GYTMYEILKFMNMAEHEILCSLRDVILQIHFTNRNFDL"
     gene            23563..24876
                     /locus_tag="PK736_01675"
     CDS             23563..24876
                     /locus_tag="PK736_01675"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC32132.1"
                     /translation="MVKDVLKLLLYDWQNRDLPVVLPRELDIDKYIKMKLNKVIVLNG
                     FRRVGKTYLLMHVAKELLKTNSRRQVVYLNFEDERIPLKTEFLTELIPTIEEMWGEAP
                     KYLLLDEVQLVPNWSKWVRRIYDQYNIRFFITGSNSKMTTVGIPTELRGRFIEKHIYP
                     LNFREYLQFKKINIEVENIEYNQRVKSKLLKYVGDYLRIGGLPEIVLSQDEDFNELVQ
                     GYFRTVVSRDIIELHNIQNVNGLKALLKILLNSKEFTVNRIFNVLKSMNHEIGKTTLQ
                     NYIGYAHDAFFLNAVEAFSFKVVKAELHPRKVYFVDNAFIKFLSTKLSDDKARFFENS
                     VAVLLQSKTEELNYFKTLNNQYEVDFVIRDHDKIKQLIQVCSDISNSKTMEREIRAFA
                     QAQGELKCKNHLLINADTDKTEKVNWFGKKIEVRMIPLYRWLLEY"
     gene            complement(25299..26861)
                     /locus_tag="PK736_01680"
     CDS             complement(25299..26861)
                     /locus_tag="PK736_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRC32133.1"
                     /translation="MKKTISKIFLGMGAMICTMAANAQWTTTGTVIRPVTLTNNVSIG
                     TTVNGANLYVEKSTAVTLGIKSTTAGATMFMDKGSATANAAFAYKLGGVTQWNSGMLG
                     NNNYSIRNVPASTFPLIIDLATDNVLINSTDIPGSGVATYPLTLKTGATTALGLDNGA
                     SIFVKNNVGNYEPFLWPQWTDNKTYMNYGAGGFDIRNSGSSTTMYMTADRKVGIGTGA
                     PSASLHVVGNEYVEGARPSFSTAVDNNMYISNSNTGYAGLSLFPSTNSLDGASIFGGT
                     GGIGLNVVVANSGVNYGPINASAFNVASDRRMKKEIVDITSDSYEKYMTYIRNIESAT
                     FRYKTETDAVRNCQHIGLIAQSLPVELQSRITESPTEQSEDRIAVNLADLSGLLVVGV
                     KALDAKQIAYEKTIADLQTQIDILKQQQSQNSNGENKLTQNTDNRLFQNQPNPFNQNT
                     IIRFALNAESQNAVIVIRDLNGVSIKQINLSKSNNGQVVINASELKQGTYTYSLEVNG
                     NSIDTKLMVVTK"
     gene            complement(27504..29780)
                     /locus_tag="PK736_01685"
     CDS             complement(27504..29780)
                     /locus_tag="PK736_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32134.1"
                     /translation="MKPSKIFSYVSLFFVLMPLLLKSQCAINPLTLLKTITPAQSDKV
                     LTYQIKHSFTLWPDSPITAKTFAISYNHAFVREPIIACENFKVKPTGQPSTFEAFCTD
                     TIDKDLPLNCFYGNKTKVDTLVRTVPLIGALYLDSACALACNSPAKSYTCIKVDSLGK
                     PSVKGTIQSFGLTQAINNDLNTATSAFSWSGVDVRDYRKLMNGTLEWVPDFGSTSKGK
                     AKSIRQKAGTSDPVVFKVHDYNTGMDTMGVLLKILMRFETEDDTITYNNYWRNDTVRI
                     ESDSASFEVYFPSPFTSKQGNIFLLVKNGVVIQSLKTGVFNTVPLPFPGTLVPFQFYM
                     KSNITFHYNLDTTGLFGNDSCDVDLLMYGHNENCYTELTDTGTVLYGYNQPSCPGTPD
                     GSINALPNFGTPPYAFVWSNGATTQTIAGLPAGAYTVIVTDILGVMKMRTIFLPPPVP
                     ITISGTVQNIKCRSAHDGSIMLNVSGGSPPYTYLWSNGKKTSTITGLYPGTFLVTVTD
                     SKGCITAATYTITQPATSVTIAKTVSKINCGGKCNANIKLTVSGGVPPYTLLWNTGAT
                     TSALTGLCAGIYTCTITDAVGCIKVNTSTVIENPLMVVTVTAINSSEASATVTGGVLP
                     HTFLWNTIPPQTTATATGLLPGKTYKVIVTDSVGCSKSKFITMPPARIGLNNIDEQVE
                     IIPNPNDGDFEVVFNFDSEFNADISIYNEMGVLIFSTQNSNLKNSNELHFDNLAKGMY
                     FIVIYNGVQLISKKVIVE"
     gene            complement(29967..33371)
                     /locus_tag="PK736_01690"
     CDS             complement(29967..33371)
                     /locus_tag="PK736_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32135.1"
                     /translation="MKNSTHIVSQQSLVKASITSVTEQGFYNLFHFLSHLRKQHLVHV
                     FKKIAIVFVLFFTTLHCAKADDDKIIKGGKLLVDSGPLAKVKLQTVNMVGEDLCDFWL
                     LWTGAVKAKSVTVNGVAVALTAKANGKQHVVFNPCVNNTKQITIQAEFEGNISLLYLI
                     PTDSMHSDLVVNDAVNPNATTVNKKIEKTTVPGGSQSPTFASSKFPSLGYTNLTGTDI
                     CDLRIESPQKIDSVFVNNKKVDSIKVGSTKVPNGTSANSKVVHVYFKCIKDGDSFKVS
                     VAAQSEITTYKITPTDSLNADVMANRSLPNNGAVEQGKRARKTNTTGGQQSPTFEPSS
                     FPRLSNSNATGTDVCDMRIKAPTGVTIDEVWVNDKKVDSIYGTDGKKITARPTLKNEV
                     HVFFPCIADGAPYSISVKFNKACTTYVYTPTDSLNADISCGIQNINPGNKATRQIKFS
                     GSTPGSTFSSSKSSTVLKNDTRDTICDLEIIAKESIDSVLVNGVKWDTKPPGKKNDTV
                     HVYAPNGGGILPGNKMVVTVYCSKKIDGYSIEITDDSDDTIYQLACTGGTMKFYLNND
                     NSAFGLTSRSGSLVAINESCSPISQLVFNSPHPGIIVDGASSKLPSYFDFPTGTLNFY
                     SPILPGAPVQFSFHINTLLPYSATDTFPYTTVNVSVPGTTISYDGTATITPASSLTEF
                     NGSIDFTLTGDMLLPSFLWSNGSTTEDISNLSSGTYTVTVSDANGCMTKVFEVPVSPS
                     VVIPNPELTLVSAGPWCSGSLNGFAMVMDNVKDNMLPLNYVWSNGATTAIINNLSAGN
                     LTVNVTNASGFSWTGTVNLTEPSLLSVTANVKKLNCNNDFSGSISLIPSGGTFPYRYK
                     WNNGTTNQTRTGLKAGTYTATVSDANGCSVVSSYQVKQPLPLQIVPTLTSVTCFGGSN
                     GSASIVGTGGTKPYTFLWTNGMTTAAVTNLTAGTYNVVVTDAKACVANSTINISSPPP
                     MQINVQPMPPSQAFANVLGGLPPYQIFWNTMPPQTGPLATGLFPGQIVHLQVMDNAGC
                     MQMQTFIMPQMKLGNTDFSSTEIGLTLYPNPAKEFVILHIDGFANESVHINLFDALGN
                     KVLSQKNLLNNGTDIVLNVMHLSNGIYTLELQLQGQTHRMKLSVHN"
     gene            complement(33655..34401)
                     /locus_tag="PK736_01695"
     CDS             complement(33655..34401)
                     /locus_tag="PK736_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRC32136.1"
                     /translation="MIKGIVIDDEKQHRDFLRDAIAVHLPGVEIIGESASVVEGIKLV
                     DSSDFDILFLDVQMPPHTGFDLLKAVTNKNYEVVFTTSFDSYAIDAIRFSAFDFLLKP
                     YGHDDLCDVIERYRQKNLQNQSTKQIENLLHNINEGTTKKKIGLSDKSGIEFFEIDAI
                     VYCKSENVYTTFYFDNKTEIVTSKSIKDYEKELSNYAFFRIHNSYLINLHKVKKYIRG
                     EGGQVVMSNGATLDVSRTRKDLFLKKLGEV"
     gene            complement(34398..36236)
                     /locus_tag="PK736_01700"
     CDS             complement(34398..36236)
                     /locus_tag="PK736_01700"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC32137.1"
                     /translation="MKNIYLLCFLILFFFSSISFSQTNKDSLLALLPSTNIDSQRVVL
                     INTLIDLIEEDTIWQPLNDEMGMIAQRMMKQNNGAINRIGTIHYAAYCNNIGFVHQQN
                     GDMPQALNFYLEGLKYYEASNDKYGIANSLGNIGTVYDEQGDWQQALVNIKQCLSIMT
                     ELKDKTGMAVALNNLGTIYNNHGLHKEALNYLEQSLALHKQTEDIENQGLVLSNIGGV
                     YDKLNDNEKAIEYYKASYDIQKSIGDDIGLCLSKINFGNIQMKQKKWSEAEKNFTDAW
                     QQSVALKYTSGMLGATDWLSRLYSATGRFEKAFNMQVLFKNLTDSIRNDDTRKQLMKK
                     QLQYDFEKREAILKLQQQQKNELANKELQKQKTLRNTIIYSAILIFSFAVFAFIFYRR
                     KREAQMKHQLADLNMRAINAQMNPHFIFNCMQSIQTLIAKKDLHNASVTLLRFAKLIR
                     KVLDHSMSKQVTLEDELETLNDYVALEKNRLEGNLIFNVTVENEIDTNETFLPPLLLQ
                     PIVENAIVHGIKSQGGSGTIAINISRILNKIRIEITDDGGGRKTQENISARKSYGIAL
                     TKERLDCMQALSNSKSDYTAENIVDVNGNVTGTKVTLLLPFTNLAA"
     gene            36523..39552
                     /locus_tag="PK736_01705"
     CDS             36523..39552
                     /locus_tag="PK736_01705"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC32138.1"
                     /translation="MKKLIIASILFLNICNAFAQTFIKIIPNNIPFDFVYSGIYTLQA
                     PQGEYIMATTVNDSAGSNIMIIKLNSAGEIILHNELLQPLFIETATNIMITQANEFVI
                     GGAYNVPNTNYDDELLMAKTDLKGNQIFMKNLTSSGICCWDFATGNIFETANHNYIVG
                     GEGFGISSINGYAKQTDSAGNNMQTFSLGSFTRTEAIVENSAGEIIMGGKDFQLNQAV
                     LTKRDANTNFKWTKTYNKGLGGTVKNTSIMEIIQLPTGFLLAAKYPNPTTGNVDLWMI
                     ETDLNGDTIKTNTFANDGNIIGMKPTYNGYAVAVIDSNNIGGYDFKIIFLDNNYNKIS
                     QRNYGTLNIDVLYSFEKCSDGGFVMSGLTYDNSFIYNALVIKTDSLGCVAPAANFTWQ
                     QASLANNQSGVDFTNTTLAGIIDTNATYLWNFGDNTFSTIKNPQHIYASQGTYIVTLT
                     VTTQCGVDSVQILVTATCTGTYNYFTHASSLLSATFNDPDPSATSWNWNFGDNTFSAI
                     KNPSHTYAATGTYYVCLTKFNTCGSFTMCDSVKIECPAPLLNIGSDKTVCQGQNVILD
                     AGNPGMNYLWSNGASTKTISVNTSGTYIVTVTNSCNAFSNDTVVINFITPPVLNFGSD
                     TTVCMNDTITFQNSAQPGFSYQWFVNSYFVTSQDSLTYAFNQIGSNDIKVIATNLGCI
                     DSAQIKVDVNPIAMCTGVPLTYCIPTYTNGTTSGDYIKRFGVNTLLNVTGSQGGPAYN
                     DYTNLKTDITGNVSYTWTFEFSVTNAMLSKMWIDFNMDGTFDEPAERVYLQAANNGIT
                     TANFSIPTSKFFGGLTRLRLVCADVANYNLIQPCGAYDHGETEDYTLNLVNPFSPPIS
                     NFMASDSTIKLNDAVNFFHLASNNATGFNWTFTGATPSTSTAANPANIVYPTSGCYQV
                     SLTATNANGNDAETKICFIDVANNIGMQEVTSNALEIYTGNNNGDFYIFNPNMDATTV
                     TVYDCMGKIIFTENTSEQKINLSLANHKVGIYFIIRS"
BASE COUNT        13037 a         7233 c         7055 g        12211 t
ORIGIN      
        1 tgtctggtgc aaaataatct tgcggcactc tgctatcgaa aatatgccgg gccaaatatt
       61 ttttatcact gcaattgatg gagacattgc cactctcttc ttgaacggat ttaacatcat
      121 cgtttatcca acttatattt tcggtgctcg aaatttttga ttttgcatga ttgtaaaaat
      181 ctatgccccg cagcattttg taatgataat ttttcatgtc gagttttatg gatttctctt
      241 ttgttataaa aaaacctgta ctccaacttt tagtaattat tttatcccaa aggccatttt
      301 cattttccca gaaacaccat gtgcggtcat ttatgttttt actttccttt tctacaatca
      361 atattttttt tgtagcgaaa taattatcgt caatcatggc caatgccaaa tgtaaacctg
      421 cggcaccggc acctacaatt gcatagtcgt atatgttttt ttgttcttcc atttttaaaa
      481 tctggtggcg aaagtatgtg tttttatttt gattgagttt taaacttgat aaacatatgc
      541 agannnnnnn nnntaatcac cttgagtaaa acaacaagaa aaaattttag caacaaagcg
      601 gctaagtcac atagtaacgc aaatgataca ataaaggtaa cgcccacttt gaaaagtcaa
      661 taaaaacaaa tagccactaa atcaccaaaa actaaagccc acaaaacaat acaaatgaat
      721 tcttgcggtg gaatgacact ttatggctta gtatttatgt ggcaaaaata ctttttcgga
      781 gtagtcttaa gatagctgca ataacaattt gtgctattgt ggcttttgag ctaaaattaa
      841 tattcggcat tcatgcgtcc tattcgtggc aaaaaagtat tcttgcattc tgaaaaataa
      901 tcattaaaaa aaatgaaaca acatttagca aaaattgctt tggtagtaaa agactatgac
      961 gaggccatag catattatac acagaaatta aatttcgaat tgaaggaaga tacaaaactt
     1021 tcggacacaa aacgttgggt tgtaatagta ccgcctggaa gtgaaggttg cgaaatattg
     1081 cttgcacaag ccgccaacga caggcaactt gccgcagtag gtaaccaaac tggaggccgt
     1141 gtatttttat ttttgtatac tgataatttt tatcgcgact ataacaacat ggttacaaac
     1201 aaaataaaat ttgtgcgacc accatcacaa gagccacatg gaacagtagc agtattcgaa
     1261 gatttgtatg gtaacctttg ggattttatc gagcctgaag gaaagtaata ttggtggagt
     1321 ttcctgattt aataaggata gacttttgtt ggccgtaatc tttgtattaa ctatgaatat
     1381 acactttggt gaaataccat cctttaaaat taaaaaataa aataaattca ttcctcatca
     1441 ctacgtcaac ttataaattt ccattatgtg ttgtagtatg tgagggaaat ctattttgag
     1501 gtcgataagt ttgccggtgt gaatatcaaa cacccaaccg tgcacagtga ggccacgttc
     1561 gtaataggct ttttgtactt cggcagtttt atactaagag cacattatat tttcattcga
     1621 aaatctaact actcttagta tataaaaaaa atgcccacgc tgtattacaa ataaatgttc
     1681 tatattagca acactaatcc taactaaaaa cattcacatg acctatttta acggaattac
     1741 cagcttcagc ctattattaa gttttatatt atgtataccg gtttcgtttt ttctggttta
     1801 catttttaaa aagattgtag aaaaaaaaat aaactcattt tcacttttca acgaggacga
     1861 acccgaaata ttggtgccca ataaaaatcc gtttccgggt ttgaaaatta tattaccaga
     1921 aaaaaacgga aaggtaatcg agggagaaaa taatttcaaa ttttttctgc cccatcttct
     1981 atcgtcaata gcagcctctg ctgcatgtat gtgcattgta tttacaattg attgtttttg
     2041 ggaaaagggt cctgtatttt ttctttttat gacagcacta catatgacac ctatattatt
     2101 ttgctttacc ctattttatc gacacaataa tgcagcaata aaaaagatgt ggctgctgac
     2161 agcgataata gtgctgatgt tttttatata ctttctttca aaaaattcga tttataatgt
     2221 attgatatta tttgccagca taggatttaa tgcagcattg attaccataa tttgtctggt
     2281 catttacaaa tttcgtaaca tacgcacatt gtttccattt gtagttacag cacttttcat
     2341 cattatatta attctcagtt ttataagcgc agtacttagt ggcaattcga atttacttgc
     2401 agcatacttc tatcctgtat acgatttttc gaaagctatt ggaatggaaa actattcaac
     2461 aactgtgttc tattttatca atattctcat tttgatatgc ggctttgctt tgggctttat
     2521 gttgcttaat tatagtttta taaaaaaagc aaataacaat acaatcatgc ttatcgattt
     2581 tgtttggctt tcgcacattc tgtttacttt aagtttgcca gttgccgata caagcttata
     2641 tatttatatg gtggcagtat ttgctgttta taaattagtt caaataattt cttttaaaat
     2701 atttattccc aaagttgaaa agggcattaa taaaacgttg ttggttttaa gggtattttc
     2761 tttgaaacac gaaagcgaga aactgttcga tatgatacgc agttattggc ttttgaatgg
     2821 cccgataaca cttattgccg gacacgacct tactgcatcg gttgttgata ttgaagacat
     2881 gctttatctg cttaccggac gcataaaaaa taggtttaac accactcccc tactcataga
     2941 aaaaaacaaa agcaagatag acaacatagc tgatattgat ggacgatatc gtgttaatga
     3001 aatgtactgc accaacacta cgtggaagag ggtactgaaa aatctgatac aaacaagcaa
     3061 taaagtgatg atggatatac gaactttcac tgaactaaat aaagggtgtg cttttgaaat
     3121 aaaagaaata atttatcgtg tacctttaaa caacatactg tttatagcaa atggcaaaac
     3181 agatttgaaa ctggcaaaaa gcatcatgga agaagcatgg aatgtaatgc cacaaacatc
     3241 gcccaatata aatgaaagcg gccaaatgat acatgttttc atgtacgaaa cacaggacga
     3301 tcttggtcaa cttattaata ctttaaaaaa gtagaaatgg aaattctgag gttgaattca
     3361 attttaagga attcaatttt ttaatagaaa tatccgtaat ttcaccttcg atttacacat
     3421 ctaaatttta aaaataattc catggctact accgaccgcg aaaaaataga caatgccttc
     3481 gaaaggctta ttgaacaata caatgctgca ctcaccgcaa ctactttgca ggatgccgcc
     3541 acagccgaaa atgcatttcg ccaggcaaca gtaatttatg cgggcataga aaagattgat
     3601 tttcctgacg aaaaatcgtt tatgagttgg gtagttgcat tcatcgaatt attgtccaac
     3661 tattcgaagg cgaacttttt attcatggaa gaacggttta aaaaatcgaa agaggagttt
     3721 gataaaacac ttttggtaat tgcatcgcta cacgaaaaac aaaaagatat aaatcaagaa
     3781 tttgttactg agtttgaact tggaaagctt tatggtttta taaatttctt tacttcatat
     3841 ttcggacacc tgacaaatgt tatgattaat ctggcattac tatcgcaaca gatgctggaa
     3901 ggaaaatata taaatgaggt tttgcttaac caaaatgccg ccaaagaaat tcgcggtttc
     3961 gattacaaaa aatatacaac cgatgatgat gatttcaatt cggaaataat aaaaattgtg
     4021 ggactgcttg gccgcatagc agatgtgtac gataaaaaag ccgaaaaagc cgaagagaaa
     4081 attaaaacaa ttgagttttt aaaaccaata gataaaaatg tttttttagt gcatggtcac
     4141 gaagaaggca accttcgtga gttgaaagat ttacttaaag ataatttcaa tatcacaccc
     4201 attattttgc gcgatgaaca agatggaggc aaaacaatta tcgaaaagtt agaagactat
     4261 ggccgtaagt gtgcatttgc atttgtgttg attacacctg acgatgtggt cgaaaacaaa
     4321 aaaacaaaag catttcaggc acggcccaat gtattgtttg aactgggctg gttttgtggc
     4381 cggtttggtc gcagcaaagt gcgcatgcta cgtaagaacg aaaccccgct tccatcagat
     4441 ttgcatggct tgattgctta tgaatttaac gaaaaagtat cggaggtgtt tttgaatatt
     4501 aaaaaagatt tggaggctgc aggaattatt taatataaat ttttaatttt cttccgcatt
     4561 tatttttcta atcaatataa aagagcttta atacaaatat cccattttgc cttgttgata
     4621 atcgcgcatg gcttcaagaa tttcggtttg tgtattcatt acaaatggcc cgtgagttac
     4681 caatggttca tggataggct caccggccat caacaaaagt ttaacgggac tcaatgcttc
     4741 taaagtaatt gcaccatccg tattttcgaa agtaacaagt tgatgttgtg tggctttttc
     4801 taaattatta atttcaatat cgccttcgag tatataaatt gcaacattgt atgacgcagc
     4861 aaattctatt tcaaatttac tattcaattg catgcgcatc atggcggtaa aaacattaga
     4921 aggtgcgggg ccttttttat caagatactt tcctgaaatt aaccgaaact gtacaccatc
     4981 tttttcaatc aacacaattt cgtttttggt gattggcatg taagatggag ttttcatttt
     5041 ttcgtcagca ggtaaattta tccatagttg tataccctga aaccggcctc cggtttctac
     5101 aaaatctttg tcggtggttt cgctgtgaat aataccacgg gccgcattta tccattgcac
     5161 ttcgttatcg ccaatcacac tgctattgcc aagagaatcg ttgtgcgaca cgcgcccttc
     5221 gtacataaat gtaacagggc aaaaaccgcg gtgaggatga ggaggtacat cgaaagaatt
     5281 gttgccaggt tcaatatcaa aatcgaaatg atgtaataat atgaaagggc taaactgagc
     5341 aaagccatgc agcggaaaag gttgacgcaa tgctatattg cccatcatca cttgtggcgc
     5401 tgtgtggata ttttttattt tccgggatgt tgtcatctta atttcttctt tcaaatttat
     5461 aatatgtgca atgtatacat gtttgcctgt ctgcaaaata gcaaataaat taaatctagt
     5521 tcatcaatta gttgagtaca cttgcaaata tacttgcgtt tgtagttatt tgaaaaataa
     5581 tatcgcccaa gggcgttgtt tttattctca cattaaattg aaaatttctt taaagcatat
     5641 cctcattctt ggaatcatta ctcgggtaat gtttctcgtt tttggcgcac ccttatatta
     5701 tggtcgcact ccattctaca tgcacgatga ttctataggt tggatggact ccatagtaaa
     5761 cttaatcaac acgggtactt acacagcaaa tgctgcatgg gccaatggtg ttttttatcg
     5821 cccaccgggt tacagttttt tcctgggctt ttgctattta ataacaggca tgtctgtaga
     5881 gcgcggattt ttgtgtgctg ttattttaca atctgctctc gatattttaa atattttctt
     5941 ttgttacaaa attgtttttc aactcacaca aaataaaaat acatcgcttg caggagcatt
     6001 catttatgca ttgtatcctt tcactttggt gtgggatgga gctgccactg ccgaaatatt
     6061 aagtgtaaca cttatgtttg cttcatttta tttctatact aacccaacac aaaaatatcg
     6121 tttagcgctc tcagcattgt ttcttggttt tgcatcgctt acaaggccac agtgcattgt
     6181 tctgttccct tgcatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccttgcatt
     6241 attgctgttg aatttatttt taatatcaaa aatccaaaaa agtatttgaa aaatatttta
     6301 caattattgt tgttcttttc actttcatat gggttgtggc ctgcccgtaa ttatatcaat
     6361 caaaataaat taatatttat acaggatgca aacataggta atgtttggtc gcccgattgg
     6421 tttgcattta tgcgctatat ttttgctgta caaaccgata ttgaaccaca agcatcgcaa
     6481 ctgatgaacg gtgaaaaagt aacatggaat gagcaatcat atcattacag tggcgatagt
     6541 gcaaaccttg catcatttac aaacatgctt gacacctgcg gaaccggagt aagttatttt
     6601 cgtttgcata aaaatttaat tgacacgcct gttgcatcca accaaaattg tgatgctgct
     6661 attgcaaaac tttcagaaga acttgttgca aatcaaaaaa aatacaatgc attcaattat
     6721 tacataaatg ttcccttcag taatttgaaa aaaagtttgt tcaaatcggg cctttatggc
     6781 aagcaaagta atagtgtgaa aataatagcc tcattgctat ttggaattcg aacagttgga
     6841 atacttgccg gtttattttg ttgcatttgg cttttacttt ttacaagcat cagaaaagaa
     6901 tttctaacca tcactattat gtactttgca gcatggtatg catacatgtg ctggttgttt
     6961 cgaaatatag aagtacgcta ttttttaaat gccgatgttt tgttattaat tccattagca
     7021 atagtaattg gtatgttaat tgacaaatat tatttacatc gcaaaacaga tgtaatttca
     7081 attgaagata aaaacagttg aagaaaaaaa accgcaacac acaatgaatg atattttaat
     7141 aatagatccg gatacaaaag cgcaactcac aaaatcgagc gagggcttgt tgcaaaacgg
     7201 tttggtaaaa tttcctttta aaaatggtgc ataccgttta gtgagtgaca gcaactatac
     7261 cgagaacttt ggaaaagaat ggaatacgtt tcaaacaaca caaatagata aacacacggg
     7321 caacaatgta tcaaaggttc gcctgtttgc acaaacgggt tggaaagaaa atgactttgc
     7381 cggagaaaat attttggaag tcggtagtgg tgccggccgc tttacccaaa tactaattga
     7441 ctataccaaa gcaaatattt ttacagttga ttacagcaat gctgtggaag caaattttag
     7501 aaacaacggt ccacacgaaa ggcttaatat ttttcagggc agtatttacg atttaccttt
     7561 tgccccaaaa caattcgaca aagtaatttg ccttggggta ctgcagcaca ctcccgattt
     7621 taaaaactcg gtacaatgct tatgtaatat ggtaaaaccc ggtggacaat tggtgataga
     7681 tttttatcca ataaaaggtt ggtactcgaa aataaatgcc aaatatatgt tgcgcccatt
     7741 tgtaaaaaat atggaccatc agcgtatgat gaagttgata gataaaaatg tggactggat
     7801 gataagtgcg cacatgttct ttaaaaaaat tggtgtgggt aaaattgtaa accggtttat
     7861 tcctgtgtgc gatataagta ccaccgtgcc caaaggatta agcaaagccc aattacgcga
     7921 gtgggttgta ctcgatactt tcgatatgat gtcacccgca ttcgacaatc cgcaaagcct
     7981 tgcaacagta tcgcaatggt taaaggaatt tggcatggaa cagattgatg catcgatggt
     8041 aaaatttgcc gaagtatttg aagctgcaac aatacgatgc acacaaaaac aataaaaata
     8101 ttttcactgc tataaactgc acaatcattg aaaaaaataa tcctcaatag tatacgaaaa
     8161 atattttgtt acgctccatt tcgaaacatt ctgcgcaggt tgacaaatgg taaaagccca
     8221 cattccataa tcggcagaat tgcgccaccg aattttttat ttgcaaaaaa ctcaattgca
     8281 aaatttaatg ccgaaggatt tgtttatgaa cttgatttga gtgattacat cggtcattat
     8341 ttatactttg gttttgagga tgatggtctt tcggatttat ataaccgcgt aagtgatggt
     8401 gcagtaatat ttgatattgg tgctaacatt ggttcaacag ctatgcactt tgctacttgc
     8461 gccaacaaca ctactgtata tggctttgag cccgatgcac ataattttaa aattgcctca
     8521 aaaaatattg aactcaataa tctgacaaat atcaaactct ataattttgg cttgggcgat
     8581 aaaaaaatgc aatcgaagtt gtttattaaa gatgataaca accgtggcat gaatagaata
     8641 ctgaatgatg aaaaaagtga attatcatac tctttgattg atgttagaac cttggatgat
     8701 ttagtagaag aaataaaacc aacaaaaatt gatgttataa aaatagatgt agaaggattc
     8761 gaaatgaaag tgcttaccgg agcaaaaaat actttgatta aatataagcc gaaattattt
     8821 ctcgaaacta ataatagctt gctcgccaac catcaatcat caataagtga tgtggtgcaa
     8881 tttttaatta cgctcaatta taaactaagc attatacccg gtaataaacc tttaacagat
     8941 gatgatataa aaggagcaaa tcatttcgat ttatttgctg aacaaaatta aaatgaaagt
     9001 aaagaatttt ttatttcatc gtgttaacga caatccggat cccttctggc ctgctatgct
     9061 aacatcgcaa ttcaaaagca ttctttcgca aattaaaaaa tcatatcatg taatcagcac
     9121 cgatgaattg ttgcatctga aaaaacatca tggcggcaaa acgcttgcca ccatttcgtt
     9181 tgacgatggc tacaaagaca atatcgagta tgctgctccc atattacaac actataaaat
     9241 gccggccaca ttttatgttg ttacaaattg catagataat aatgtgccaa catggactta
     9301 cgatatagat taccgcacat actttacaaa aaacacaatc gatttctctt cgtgcaatct
     9361 tcccgaaagt ctgctacaca aacaaagaga taaaaaaact tctaccgctc aatttaccgg
     9421 ggcattaaaa gcatatttaa aaaaaataga ttttaataaa gttcttgaaa ttgtaaaaac
     9481 cattgaagaa caaacgcaag atatagacat gccaaaactg atgatgcatt gggacgatat
     9541 tatgcaactc tacaaaagca attttatcat cgggtcgcat tcacacacac atcctatgtt
     9601 gaataatatt acaaatgaaa atattgtgcg ggcagaattg gttatttcaa aatcaagaat
     9661 tgaagagaaa ataaacagcc acatcagctc tattgcattt ccggttgggg gctataacga
     9721 aactgtaaaa actttaagtg ccgaagaagg atataaatat gcgatggctg tagaacaaaa
     9781 atcgcatggc gaaaatatag atttgtttgc cattccccgc atagagttgt acaatgaacc
     9841 gcgctggaaa acttttttgc gcatgaatga agtaatgcag cgaacaaaaa aaattgtgcg
     9901 ccatggataa gccggtaatt ttaatgctat gcaataatgc acacaaccaa attgcattgg
     9961 ctaataaaat gcacacttca ttgggcattg accatataat tgtggaaaat aaaatcacta
    10021 atcgaaagct atcgcttcaa aatatctgga acaaacttcg ttataaaact attgatgatg
    10081 catggtttgc tatgttgtca ttttacaaaa ataaatttcc tgcttttccg gcaacaaatg
    10141 tttctttcac tattgatata aattctgccg aaacagaatc aataataaaa acactaaaac
    10201 ccgatttgat acttgtatcg ggaacacgaa taataaaaaa taacatcatt ggcattaatc
    10261 ctccgttagg aataatgaat atgcacaccg gcctatcgcc atttgtaaat ggtggaccta
    10321 attgcactaa ctggtgtatt gcgctcaata aatttcatct gataggaaac acaattatgt
    10381 ggctcgatgc cggaatcgat tcgggtaaca ttattacaac atcgcaagtt gatttcacag
    10441 gaaacgaaac gctgactgat ttacaaatta aagtgatgga acatgcacat cagttataca
    10501 ttgttgctgc aaaagcagta atagcaaata agaattcggt aaacaacatt ccacaaaaaa
    10561 gtataagcac aggcaatact tattacacta aaatgtggac aagcaaacaa aaaaaagatt
    10621 tgttacaaaa ttttaagcac tttgcgaatg tgataaaaag cgaatcgtat ttgcaaaata
    10681 aaaataaaat aaaaactata cccttataag atatgtgtgg catagcaggt ttttatgata
    10741 acaaaaatac tggtgacgaa gcattgctaa gaagcatgac tgtgtcgcta gcacatcgcg
    10801 gtcccgatgc cgaaggattt ttttataacg gcaccattgg ccttggtcac agacgcttga
    10861 gtattatcga cttggatata cgttcgggtc agccattcta ttctcaaaac aaacgataca
    10921 ttattatttt caatggtgag atttacaact acatagaact gcgcgaacaa ttgaaacaaa
    10981 accacagcat tgcttttaat acaacaagcg ataccgaagt gttgatagaa tgctggaata
    11041 tttatggggt agaaacacta caaaaactca atggtatgtt tgcctttgca atttacgaca
    11101 ccgaaaaaaa agaaattacc attgcccgcg accattgtgg aataaaacca ttgtactaca
    11161 cggttcaaaa cgaggccatg ttttttgcct cggaactaaa ggcattaaaa aaagtaaaca
    11221 cattaaattt cgaaattgat actacggcca tagcagctta tctgcatgcc ggttgtgtgc
    11281 ctgcaccatt gtctatctat aggcacatta aaaaattgtt accgggccat tatgttacat
    11341 atggcaaaaa cgggctacat acttttcaat attattcgct cacaaaaaat attgcctcac
    11401 attatttaac cgattataaa agctcaaaac aacatttaga ttcactgcta aatacatctg
    11461 taaatattca gatgcgcagc gatgtacctt ttggtatttt tttaagtggc ggtgtcgatt
    11521 caagtttagt taccgcactg gcagtaaaaa attcgcaaca taccatcaac accttttcca
    11581 taggttttgc cgagagtagt catgatgaat cgaagtttgc acaacaggta gcgagtcatt
    11641 taaaaacaaa tcaccatcct ttcgaggtgc attacaaaaa tgctatggag ctcgcttcac
    11701 aaatcacatt gatatacgat gagccctttg ccgatccttc ggcaataccc acttacatgg
    11761 tttcggaact ggcgcgtaag catgttacta ttgccctggc aggcgatggc ggtgacgaat
    11821 tattttttgg ttacggcagt catcagtggg cagcgcggtt atcgaaaccg gcaatgaaat
    11881 attcgcgcca cgcaattgcc aaaaccatga acatgatgtc atcgcgttac aaacgtattg
    11941 cgagattgtt gcacgaaccg gcactttcaa attttcattc gcacattttc agtcaggagc
    12001 aatactattt ttcggaaaaa gaaatttcgc aattcgtgaa aacaacaaaa tcgtttgagc
    12061 ttatactcga ggctaaagat ttgcacttcg aaaaaaaatt caaccgtaaa ttatcgtcaa
    12121 tcgaaaaaca ggcattgcta gatttctatc tttacctgcc cgatgactta cttacaaaaa
    12181 ccgatagagt aagcatgaag cattcattgg aagtacgtgt gcctttgctc gatcacaata
    12241 ttgtagacta tgccatgaat atgcctaccg actggaaagt gaaaaatggt gtagcaaaat
    12301 ttatactcaa agatatttta catgattatc ttccatcaca atatttcaat aggcccaagc
    12361 aaggtttctc tattccactt gcaagatggt taaaaaacga tttgcatttt ttaattgacg
    12421 acaacttaaa tgaaaccatc ataaataaat ttggtgtact tgattcaaaa tatgtaacag
    12481 gtcttgttaa aaaatttaat gatggcaatc acgattttct tttccaacga ttgtggaatt
    12541 taataatatt gcaacaatgg ctacgcaaca attgaataaa gctgcattta gtatcgattt
    12601 agaagattgg tatcagggaa ttgaaaaacc ctttgccgac tggccatcgt atgaaacccg
    12661 catcgaaaaa ggattttacg ccatatttaa tctcttgcaa aagcacaata caaaagcaac
    12721 atggtttact ttgggttggg ttgccgaaaa atatccacac ctgataaaag aattgcatgc
    12781 cgcagggcat gaacttggat cgcacacata ctcacacgaa aaagtgtata accaaactcg
    12841 tgatgagttt cgcgaagaga taaaaaaaac aaaacaactt atagaaaata ttactggcaa
    12901 agaaatagtt gctcaccgtt ctccgttttt ttctatcacc aacaaaagtt tgtgggcact
    12961 tgatatattg aaagaagaag gctacacaat agattgcagc atatcgccca taaaaacatg
    13021 gcgctatgga atttcaacat gccccgacaa aatatttagt ataaaagaaa acggcttgat
    13081 agaatttcca gtttcgaggt ttaaattttt gaaaaagaat tgggccattg gcggagcata
    13141 ttttcgtatt tttccttaca actttacggc tatgggaatg cgaaaccgcg ataaagaaaa
    13201 tctgccaaac atgttttata ttcatccctg ggaatatgat gcaaatcatc ctaaagtgcc
    13261 aatggaatgg aaagcaaaaa caacacacta ttttaatctt tcaaaaacaa tttcgaatac
    13321 cgataaatta cttaacgaat ttaagttcga cacagtaagt aatattatag aacaatatgc
    13381 aaagcacaat accatcgaac aaatcagcat tgacgttttg caagattgat aatcgagcgg
    13441 acaatagtga tttaataaaa tatgttgccc aggttcttgg cgaaaccaat tcgcataagg
    13501 ctacaattat gaccaatgaa ttttggaaat ggcaacactt gttattaccc ggtggcgagg
    13561 catgcgttta caaatcaatg tacgaaaata aaattgccgg atattatcat gtgccggttt
    13621 atccatcgtt gataaatggt gaggcagcaa aatttgctat gattcaagat gttgctgtta
    13681 acagtgaaat acgcggacag ggcgtgtttc ggcagttggc aacatttgca aatagcgatt
    13741 tggacataat ggatttcgat gccatctaca catttccaaa tacaaaaagc aaacacactt
    13801 ttattaagta taatggcttc acgcacttac atacatttac tgcatacatg ctaccggtaa
    13861 atgttggatt gattgcaaaa gcgaaaacaa aaattccgtt tttatttgaa acgctgtttg
    13921 cgcccattaa ctggatgctt ggcccacgaa aaaaaagtga ttatagtttt accgaaataa
    13981 ataaattcga tattgacgtt gtaaaacttt ataatgagta ttgcggtgca tataaattct
    14041 atgcccatcg aactgcggaa tatttaaact ggagattttt acaaagacca tcgtcaaaat
    14101 atttttgctt tgcagcaaaa actaacaatg cattaaaagc catagcattt tttaagattg
    14161 atgtaatgat ggatgtgccg gttctgttat tgatagacta tgcacacaat gatgaacgag
    14221 cattttcgca attggtaaat tatgtttcgg caaatgcact aaaactatgt ggctcaaaaa
    14281 ttgcttttac atttacatct gtttgcgaca aaaatttcga tgctcagaag ccacaaggat
    14341 ttattaaagt tcccgaaaaa gtaaatccgc gtttgctcaa tctgttgata agaccattga
    14401 agtataagca gcaggatatt tttgatgcca acaactggtt tgtaacttta gctgattggg
    14461 atgtatttta aatttctact ttgcacaaaa taaattttac actttgccaa aaattctacg
    14521 cataataaac agactaaatc ttggaggccc cacatataat gtaggatatc ttaccaaata
    14581 tcttgcgccc gaattcgaaa ctattttatg cagtggtgta aaagatgata ccgaagaaag
    14641 ttcggaattt attatcagcg agctgggcat tacccctcat tacataagca ctatgcgcag
    14701 agagttgaac atagttgatg actatacagc ttacaaagaa ataagcgaac tgataaaaaa
    14761 gcacaagccc gatattgtac atacacacat ggcaaaaccc ggagctgttg ggcgtttagc
    14821 cgctatacat aacaatgtgc cggttgtatt gcacacgttt catggccact attttcactc
    14881 ctatttttcg ccactaaaaa ccaaattatt taaatctata gaacaatacc ttgcgcgtaa
    14941 aagtgccggt atagttacca tcagcaaatt gcagcagcag gaattatcgg tgcagtttaa
    15001 aattgctcct cccgaaaaaa cttttcttgt tcctcttggt ttcgaccttt cgcgctttag
    15061 cgagaacatg actgaaaaaa gaaaatcttt cagaactaaa tatggaattg ccgatgacga
    15121 aatcgcaatt ggcattattg ggcgattggt tcctataaaa aatcacaaac tttttttacg
    15181 tgctatttca gaagtattaa agaaaacaaa taaaaaagtg agagcggtga tcataggcga
    15241 tggcgaaaat aaaaaagaag tgattgattt atgttttcgt tataaacttg ctttcggtac
    15301 cgataatttt gaaacagaaa accatccggt aatttttaca tcgtggtttt tgaatatcga
    15361 tgaagcactt gccggattag atatagtagc actcacatcg ctcaacgaag gcacacctgt
    15421 aagtttaata gaagcgcagg ccgcttcgaa acccatagtt agcaccgatg tgggtggtgt
    15481 gcgcgatgta gtgctcgaaa atgttactgc actgttatcg cccagtgaga atgccgaaat
    15541 actgacaaaa aatattttgg cattggtaga ggatgagcac ttgcgcacaa aattttcgca
    15601 agcagggccg tcacatgtaa ataaaaattt ccactacaca ttgcttgtcg aaaacatgcg
    15661 cagcctttat cactctctac ttaataaatg agcaaaacca ttctatactt atcgtacgat
    15721 ggaatgaccg atcaacttgg acaaagtcag gtgctgccat acctgtgcgg gttaagtaaa
    15781 aaaggatatg ttattcatct tgtaagttgt gaaaaaaaag aacgcttcga agtccacaaa
    15841 aatttgattc aaaatatagt tgatgaaaat aatatcatgt ggtatccaat taattaccac
    15901 aaaagccctc ctatcctctc aacactattc gatataagac aactgataaa aaaggctgaa
    15961 gaattgcatg cgatttacaa attcgatgtg gtgcattgcc gcagttatat aacttccttt
    16021 gccgggcaat ggatgaaaaa aaaatacgga acaaaattta tttttgacat gcgcggtttc
    16081 tatgccgatg agcgtgttga tggtaacatc tggaaacaaa gtaatcccat tttcaaattc
    16141 gtttataatt atttcaagaa taaagaaaaa caatttttgc aacaggccga tcaaatagta
    16201 agcctcaccc acaatgcgaa aaaaataatt gatgaatgga aacttaacgg gaataattct
    16261 ttgccaatca acgtaattcc atgttgtgca gatttatcac atttcgatta caataatttt
    16321 acggcagatt tcatggataa aaaaaaacaa tcaatgggat atattagcga tgataaaatc
    16381 atggtatatc ttggagcaat aggcacatgg tatatgctac cgcaaatgct cgattgctat
    16441 aaaatattcc gaaaaaataa tcaaaaaata aaattacttt tcatcactaa cgaaccgggt
    16501 gaaataattc taaaacaagt aacccaaaaa agcatcgaca tcaatgatgt aaaaataatt
    16561 tcggccacac gcaacgaagt gccaccgtta ttgagcattt gtcaatactc catttttttt
    16621 attacaccta cattcagcaa acaggcatcg tcaccaacta aacaaggcga aattatggga
    16681 atgggcattc ctatcatttg caatagcggt gtaggcgata ccgattatat aattgaacaa
    16741 acacattcgg gagtgttggt accacaattt acagatttag aatatcaaaa agcttatgaa
    16801 agaattgaaa atacatcttt cgaaaaggcg aaaacaaggc aaggcgccaa ccaattttat
    16861 tctttacaaa aaggagtaga agcatatgaa aatatatata agaaattata aaaggtaagt
    16921 caaaagagtt aaggtaaaag gcaaaagaaa tcaatcaaaa gttttaaatc aaaagagtta
    16981 agtcaaaaat aaattctcaa cgaacaaaac taaaaacaag aacaagccct ccgtacacga
    17041 aactaaaaat ttcagagtcc tcatttattt ttttttcaat ggcttgagag aatctgaaat
    17101 gaatacaaat tctacacaga gattgcctcg cagttcgaga atactcactg tgacaattcg
    17161 caatgacgaa cgaagaaaaa tccgctccgc aaacgtaacg aagaacttgg cgcatcgaaa
    17221 atgtaacgaa gaacgaagaa caatccgctc cgcaaacgaa actaagaact aaaaacaatc
    17281 cgctccgcaa acgaaacgaa gaactaagaa cttggcgcat cgcaaaccta acgaagaact
    17341 aaaatctaag aactaaaaaa aaaactcgtc atcgaaattg aaccggccac tacagagtcg
    17401 ttaaaaaatt caaggctatc tttttcattt tgcatggcca acgtatacag cataggtaga
    17461 taatgttcgg gagtaggaat ggcgagcgat gcggcctttc caaacgaagt atagttaatc
    17521 agtgattgat ggtttttatt ttcaatatcc tgtttcaatt tagaattcaa ttctattgcc
    17581 cagtcgtatc cataaggttc atcgaagcta ttccctttaa aactcaacat gccaaggttg
    17641 tgaatcatat tgccgctgcc tataataagc acacctttat ttcgcaaggt ttttaattgt
    17701 tgggaaaggg cataatgttg ggacgcagtt tgagtgtagt ccaaactcaa ttgaacaata
    17761 ggaacatcag ccagcggaaa catgttcata gccacactcc agcaaccatg gtcgaaaccc
    17821 catttaccat cgaaagcgat ttggtttgat gctactgctt tttgagtttc acctgccagc
    17881 catgtactgc ccggtgccgg gtattgtgct tcgaatagtg cttgcggaaa accaccaaaa
    17941 tcatgaatag tctgtggcct ttcttgaatg gttacaaaac tgccttttgt ttcccagtgt
    18001 gccgaaacca ttaaaattgc ggtaggcttt cccattctct ttccaatagt tttcatctca
    18061 cgcgaaaaac gattatcttc aatagcattc attggactgc catgcccaac aaacaagaca
    18121 ggcatagatt cgcttttggc aaatgaacca gcagcttttg ccaaagattc taaactggta
    18181 atacttcccg caattggtaa catggtcaat gattttaaaa aattcttacg attcatgatc
    18241 agaaataata ataacaaagt aaaaacaaaa tgcagactat ttgaaataag gtttattgtt
    18301 gttaaattct tagaaaacga aaaaaaataa aatccagttt gtacttagtg ttaataatac
    18361 actatcttcg ggacttaatt tttttaaagt atggtaaaag accaaaacaa acctataaaa
    18421 aacattggcg tttttacttc gggaggcgat ggccccggta tgaatgcatg catacgcgca
    18481 gttgtgcgca ctgccattta ccacaacgta caggttacag gtataatgcg tggctacgaa
    18541 ggcatgattg acaatgaatt tatccccatg gatgcacgtt ctgtgagcaa tattattcac
    18601 cgtggcggta ctattttaaa aactgcacgg tgcccacgtt tttttgaata tgaagggcga
    18661 aaaaaagctt acgaaaattt gatgtccaat aatattgatg gggtagttgc aattggtggt
    18721 gatggcacgt ttaccggtgc cgaaatattt tttgaagaat ttggtgttcc tatgattggt
    18781 acacctggca ctatcgacaa tgatttgttt ggtaccgatt ataccattgg atacgacacc
    18841 gcagtgaata ccgccattga agctatcgat aaaattcgtg atacggcagc ggcacacgat
    18901 cggctatttt ttgtagaagt aatgggtcgc gactcgggct gtattgcgct tgcagctggc
    18961 ataggtggcg gagcggaata tattttattg cccgaacgtg atactacatt agatcaatta
    19021 atagaagtac ttgaacgtgg agccaaaaat aaaaaaacat caagcattgt agtagttgcc
    19081 gaaggtgaac atggtggagg cgcatacaaa attgccgaaa aagtaaaaga aaaatttaag
    19141 cattacgata ccaaagttac tgtgcttgga catgtgcagc gtggtggttc gccaacttgt
    19201 ttcgaccgtg tgctggcaag tcgccttggt gttgcagcgg tagaggcttt gcttggtgga
    19261 gagtcgcgta aaatggcggg attaattaat aacaaagtag tgctcactcc attcaaagat
    19321 gccacatcac aagattttca gttagataaa tcattattga aaattgccga aatattagct
    19381 gtttaaataa caaacaaaaa tccatagaat atgaatttcg attttcccac gattgatcag
    19441 attcctgaag aatataaaat tactacaccg gttgaacaaa aagtttacct caccaatggc
    19501 gaacttattg aatggaaggg agaaacggca gatgtgtttt ctcccatcta tataaaaaca
    19561 caaccatcgc tgtatacgca gtttcgtata ggttcgcaac ctgtgatgaa taaagaagcc
    19621 actctcgctg tgctcgacaa cgccttgaag gcttacgaca atggccgtgg cgaatgggcc
    19681 accatgcgtg taaagcaaag aattgaaatg gtttcacatt ttgccaaacg tatgaaagaa
    19741 aaacgtgacg aagttgtaaa actgttgatg tgggaaattg gaaaatcgtt gcccgattcg
    19801 caaaaagaat ttgaccgtac tatcgattac atacaagaaa cgattgatgc attaaaaaat
    19861 ctcgatcgcg atagctcacg aattcaaagc aatggcggga tatacgcgca aatcagaagg
    19921 ggaccacttg gtattgtatt gtgcatgggc ccatacaact acccactcaa cgaaacattt
    19981 tgtacattga taccggcact cattatgggc aatgtggtaa tattcaagcc ttcaaaatat
    20041 ggtgtgttat tgcattcgcc tttactgcaa atattttgcg attgctttcc aaaaggagta
    20101 attaatatca tatacggcaa aggttccgaa acagcaggct cacttatgga aacaggaaaa
    20161 atagatgtgc ttgcatttat tggtaccagc cgggtggcaa atattttaaa gaaacaacat
    20221 cctcaaccca atcggttacg aggcgctttg ggtcttgaag ctaaaaatcc tgcaatcatt
    20281 ctgcccgatg ccgatattaa aataactgtt gatgaattga tagcaggttc attatcattc
    20341 aatgggcagc gatgcactgc actcaaaata atattttgtc atcatagtat acagtcacag
    20401 ttagttgatg cttataaagc caagcttgca acgttggttg ccggaatgcc gtgggataaa
    20461 aatgcgatgt taactccatt gcccgagaaa ggcaaaactg cttacttgca agagctaata
    20521 gatgatgcca ttgctaaagg cgcttcgata ataaatgaaa acggagggga aatattctgt
    20581 tcttatatgc atcctgcagt acttataaat gtaaacagca ccatgcgcat ttatcacgaa
    20641 gagcaatttg gccccgtagt tccaatagta acattcagca atataaatga gcccatcaat
    20701 tatatggtgc aatcaaacta tggtcagcaa gtaagtgtgt ttggaagcaa tagtgtagaa
    20761 attgccaagc ttatcgatcc gctggcaaat caagtttgtc gtgtaaatat taattcgcaa
    20821 tgtcagcgcg gccccgatgt gtaccccttc aatggccgca aagattctgc cgaaggaact
    20881 ttaagtgtta atgatgcatt acgtgttttt tcaattcgca cattggtagc tgcaaaaaac
    20941 agtgacctca ataaacatat agtttctgaa atattagaac aacgcacgtc caattttctg
    21001 aacaccgatt tcattttgta aaataaaaag atacatattt gtaatactta aataaacatt
    21061 atttatgatt ttagttgctg atagtggttc taccaaagcc gattggaaat tggcttaccg
    21121 aggaaaagaa atggttacat ttaatagcat tggtcttaat ccattttttc atgatagcaa
    21181 ttcagtagct gcaaccatgc aagttatacc ccaagtgcaa caatatgcca atgaagttca
    21241 tcgcatcgaa ttttttggcg cgggatgttc tcaccctgca cgaaataaaa taattagtga
    21301 tggactacaa attgtttttc caaatgcagc tataaatatt catcacgatt tggatgctgc
    21361 agcttatgct acatgcaacg acaagccggg tattgcctgc atactcggta cgggaagtaa
    21421 ctcatgttat ttcgatggca caaaagttca tgaattaaac cacggtctgg gatatatttt
    21481 gggcgatgaa ggaagcgggt ccttctttgg ccgtaaacta cttgcttatt atctgtacaa
    21541 agtaatgccc gatgacttgc gcacgcaatt tttcgaaacg tataaagtca ctaaagaaag
    21601 catcatgcaa caagtgtatt accagccaaa tgcaaacgtt tatttagctt ccttcagccg
    21661 gtttctttca gttaatacta accatacttt tattactaat cttgtaagtc gagggttagg
    21721 tgagtttatg gatgtgaatg tttgcaatat cgaaaattac aaaaacgtac cagtgcattt
    21781 tattggcagc attgcatatc attttcaaaa tatacttaaa caagaagctg caaagcgtag
    21841 cattaccgtg ggcaacataa ttgcgaagcc gattgaaagt ctaactcaat attttcttga
    21901 aaaagaaaat ttgcagaaat aatatttcaa tacatgaaag aaacaaaaca tttcatcttg
    21961 cttcaaacgg cttggtcccg ctttccactc atagttgcaa ccattttcta ttttttcatt
    22021 ggcatggttg caagctaacc gttacaatca gggctattta caatagtatc gttattcaaa
    22081 ggatggtata acaatcaata caattggatt gaaatacgtt ttaaaactta attgtcattg
    22141 ttaaaaaata aattggcgtt aaattaccat cataattttt taaaaacaga aagtgcaaaa
    22201 tgactaccga aatattcgtg gcacaatatg tcttcaatca gtcaatccac actttaataa
    22261 atgaactata tttgtagtct aaaaaaaatg aaatggaatt agataaatta aaatacccta
    22321 taggtaaatt ccagtacact gagtcagcaa ctgccgaaca aataaagatg tggatatcac
    22381 aaattgaagc attgcccgaa aaattaattg caacggtaca ccaattaacc gaagcacaac
    22441 tcgatactcc atatcgtcct caaggatgga cagtgcgaca agtaatacat catattgctg
    22501 atagtcatgc aaatgcctgc atacgttttc cgcttgctct taccgaagat aacccggtga
    22561 taaaacctta ccaagaagaa ctctgggccg aattggaatt tcaaaaaaaa ctgccaatat
    22621 caaattcatt gcaattaata aaattgttgc atcaacgctg ggtggtgtta ttaaatacaa
    22681 tgacagaaaa agattttgca cgcacatata aacatccgca atatggaaaa gaatattctt
    22741 taacggaagc attaggactt tacgcatggc atagtaatca tcaccttgcg catattacaa
    22801 atcttataga tcgaaatttc gatttgtaaa atgtatttgt agaattacat ccctgaggct
    22861 acataatatt tcatgctcag ccatattcat aaatttcaaa atctcataca tggtatatcc
    22921 gcctgaaaat aatgccaaca tttgcatgtt tcgtatacta acttttccaa tccgttgcat
    22981 taaaatattc tcatttatta gttgcgctaa aggcaaacta acgcggcttt cacctttgta
    23041 tatttctctt atcgttctta taaaagattg tgctttcaca aatttaccaa tatgaccaat
    23101 tgctccagcc ttgatacagt catatactaa atcatattga atccaggcag ataaaatcac
    23161 cattctgatt tcaggatgct gtttaactaa ttttaaaata acagtttcag catagccttc
    23221 tgtactatga acatcaagca atattaaatc atttttatgt gaatcaatat tgcttacaaa
    23281 tgcttttaaa ttggcataac acccaacaat aacaatatcc tgtaccttgt aaaatatgct
    23341 ttgataatat ttatgtacaa ctgaatccga ttctattatt acaatgttaa tggtgaccat
    23401 ctcgattatt ctttcataag caaataagta tataaaagca gaaattacaa taccgcagtt
    23461 ggggtatatt taaagcttca aaattttttt tggcaatatg tcctacatat aagacatatt
    23521 tatatatatt tgtcctatca aatatttata agaagcagga tcatggtaaa agatgtttta
    23581 aaattattgc tgtacgattg gcaaaatcga gatttgcctg ttgtgttacc gcgcgaatta
    23641 gatatcgata aatacataaa aatgaaactc aacaaagtaa ttgtgttgaa tggatttaga
    23701 cgtgtaggta aaacttactt actaatgcat gtagctaaag aactattaaa aacaaatagt
    23761 cgcagacaag tagtttatct taattttgaa gatgaacgta taccattaaa aacagaattc
    23821 cttaccgaac ttataccaac aatcgaagaa atgtggggcg aagcacctaa gtatctttta
    23881 cttgatgaag tgcaacttgt tcctaattgg agtaaatggg tacgaagaat atatgatcaa
    23941 tataatatac gtttttttat cacaggcagc aattcgaaaa tgactacggt gggtatccct
    24001 acggagttgc gtggccgatt tatagaaaaa catatatatc cattgaactt tcgcgaatac
    24061 cttcaattta aaaaaataaa tatcgaagtt gaaaatatag agtacaacca acgggttaaa
    24121 agtaaattac taaagtatgt tggtgattat ctgcgtatag gcgggctccc cgaaatagta
    24181 ttatcacaag atgaggactt caatgaactg gtgcaaggat acttccgaac ggtagtaagc
    24241 cgcgatataa tagagttaca taatattcaa aatgtaaatg gcttgaaggc cttgctaaag
    24301 atacttctga acagcaaaga atttactgta aaccgtatat tcaatgtttt gaaaagcatg
    24361 aatcacgaaa taggaaaaac aacattacag aactatatag gttatgcgca cgatgccttt
    24421 tttttaaatg cagtagaagc gttttcattc aaagttgtaa aagcggagtt gcatcctcgc
    24481 aaagtatatt ttgttgacaa tgcattcata aaatttttaa gcacaaaact aagtgatgac
    24541 aaagcacgat tttttgaaaa ctcagttgca gtattgttgc aaagcaaaac cgaagaacta
    24601 aattatttca aaacactaaa caatcagtac gaagtagatt ttgtaattcg cgaccatgat
    24661 aaaataaaac aacttataca ggtatgcagc gatataagta acagtaaaac aatggaaagg
    24721 gagattcgtg catttgcgca ggcacaggga gaattgaaat gcaaaaatca cttactaata
    24781 aatgccgaca ctgataaaac cgaaaaagta aactggtttg gcaaaaagat agaagttaga
    24841 atgattcctc tttatcgttg gttattggag tattgataat gtcttatgca taggacatat
    24901 ttacataaat ttgtcctatg tattttcaaa aattagattt tttatcaaac attaattgaa
    24961 caatactccc gaagcaataa caacacaacc attatcaggc gcctttgaag aaatgactaa
    25021 tagttaatct aaaatattca gtaataaaac ttgaatcacc ataaattttg aaacaaaaaa
    25081 accggataaa atttctttca tccggctctt ttttggttac tgattatttg gttattgagt
    25141 tatttgttac tggattactt tgttattgcg ttgtttttca ggaatcgcat tgttggttaa
    25201 aataatgttt ttgttgaaaa tactaagatg aatgtgcttt agatttattt caaaaaagca
    25261 taataaccca ataacataac aacccaacaa cccaataact acttagtaac aaccattaac
    25321 ttagtatcga tactgtttcc atttacctct aacgaataag tataagtgcc ttgttttaac
    25381 tctgaagcat tgataaccac ttgtccatta ttagacttgc ttaaattaat ttgctttatt
    25441 gaaactccgt ttaagtcacg aattacaatc acagcatttt gactttcggc atttaatgca
    25501 aaacgaataa tggtgttttg attgaatgga tttggctgat tctggaaaag gcggttatca
    25561 gtattttgag ttaatttatt ttctccattt gaattttgcg attgctgttg tttcaatata
    25621 tcaatttgag tttgcaaatc agcaattgtt ttttcgtaag ctatttgttt tgcatctaaa
    25681 gcttttaccc ctactactaa tagcccagat aaatctgcga gatttaccgc aattctatct
    25741 tcgctttgct cagttggcga ctctgtaata cgtgactgca attcaaccgg taatgattgt
    25801 gcaattaaac caatgtgctg acaattacga acagcatcgg tttctgtttt gtaacgaaat
    25861 gttgccgatt ctatattgcg tatataggtc atatactttt cgtaactatc agatgttata
    25921 tcaacaattt cttttttcat tcttctatcg ctggcaacat taaatgcgct ggcattaatt
    25981 ggaccataat taacaccaga attagcgaca accacattca aaccaatacc gccagtacct
    26041 ccaaatatag aagcaccatc taaactattg gttgaaggaa ataaagaaag gcctgcataa
    26101 ccggtgttgc tattcgaaat gtacatatta ttatcgacag cagtagaaaa acttggacgt
    26161 gcaccttcaa catattcgtt acccactaca tgtagacttg cacttggagc tccggttcca
    26221 atgcctacct ttctatcggc agtcatgtac attgtagttg aacttccgct atttctaata
    26281 tcgaaaccac cggcaccgta attcatatat gttttattat ctgtccactg tggccataaa
    26341 aaaggctcat aattgccaac attattttta acaaatatac tcgctccatt atctaaaccc
    26401 aaagcagtag ttgcacccgt ttttaaagtt agcggatacg ttgcaactcc cgaaccggga
    26461 atgtcagtag aatttattaa tacattgtca gtagccagat caataattaa tgggaaagtg
    26521 cttgctggca cattacgtat actgtaattg ttgttaccca gcataccgct gttccattgc
    26581 gttacaccgc caagtttata tgcaaatgcg gcattggcag tggcactacc tttatccata
    26641 aacatggtag caccggcagt tgtcgattta attccaagtg taactgcagt cgatttttca
    26701 acataaaggt ttgcaccatt tactgttgtt ccaatactta cattgttagt aagcgttaca
    26761 ggacgtataa ccgtaccggt tgtggtccat tgtgcatttg cggccattgt gcaaatcatt
    26821 gcacccatgc ctaagaaaat tttggagatc gtttttttca taacattttt ttgttaaaga
    26881 ttaatattga gattagagac ttatttctta aaaatttgaa cataccacac cacctacact
    26941 tagtgatatt tttttgtgtt cgaattaaaa tctaatttcg gtagtgaggt taattttggt
    27001 ttttcaatac ctgaattgaa ttagtgagaa caaacgtaat atagaaaacg aaaaaagcaa
    27061 gaactatttt tcagaagcgt cataactctc tcaaaatcaa cacaataatt ttcgtttttt
    27121 agttatcaaa attcaaaata atattcaaac gcgttttttt aaataataaa gtagttgtcc
    27181 aaaaatataa tctcaaatct attttcagaa tagggttgtt aatatattga aacataagat
    27241 gctgaagtga aaattaatct gtcctggcct tgtaatccaa taatataagc aggtttggct
    27301 ttttgtccag ttcacttgtg tctttaaaaa gaatgaattt aaatgctgca aaacattacc
    27361 tatgccattg ttagcaaagt acaatagata tatttacaca gaaccaattg tcaaaaagcc
    27421 aataattttt tatttctaaa aagttgaatc gccaatgaat tcccgtacaa aaaaaccgga
    27481 gcaaaaatct aaccctactt ttctcattca acaattactt ttttggaaat aagctgaacg
    27541 ccattataaa tgacaataaa atacattcct tttgcaagat tatcaaaatg aagttcgttg
    27601 ctatttttta aatttgaatt ttgcgtagaa aaaattaata ctcccatctc attatatatg
    27661 cttatgtccg cattgaattc gctatcaaaa ttgaatacaa cttcaaagtc gccatcattg
    27721 ggattgggaa ttatttctac ttgttcatca atattattta atcctattct tgcaggaggc
    27781 atggttataa atttgctttt gctacatccc accgagtcag taactataac cttatacgtt
    27841 ttgccgggta aaagtccggt agccgtggca gtggtttgtg gcggaatggt attccataaa
    27901 aaagtgtgag gcaacacacc accagtaact gtagctgaag cttctgagct gttaatagct
    27961 gttactgtaa caaccatcaa tgggttttcg attaccgttg aagtatttac tttaatacaa
    28021 ccaactgcat ctgtaattgt gcaagtgtat atgccggcac ataagcccgt taaagcagaa
    28081 gtcgtggctc cggtattcca aagtaatgtg tatggtggca ccccaccact tacggtgagt
    28141 tttatatttg cattgcattt accaccacaa tttattttcg atactgtttt tgcaattgtt
    28201 acacttgttg ccggttgtgt tatcgtgtag gttgcagcag taatgcatcc tttactgtcg
    28261 gtaacggtaa ctaaaaaagt gcccgggtat agccctgtaa tagttgaagt tttttttccg
    28321 ttactccaca aataagtata aggaggagag ccgcctgaaa cgtttagcat aatagaacca
    28381 tcgtgtgccg aacggcattt tatattttgt actgtaccac tgattgtaat tggtactgga
    28441 ggaggcaaaa atatcgtcct cattttcatt actccgagaa tatctgtaac tataaccgta
    28501 tacgcaccag ccggcaaccc ggcaatcgtt tgtgtagtgg caccattgct ccatacaaaa
    28561 gcatagggtg gtgtgccgaa attgggcaat gcatttatac taccatcagg tgtgccggga
    28621 catgaaggct gattgtatcc atacaaaacc gttcccgtat cagtcaattc tgtgtaacaa
    28681 ttttcgttgt gaccatacat aagcaaatca acatcgcaag agtcgttgcc aaataatcct
    28741 gtggtgtcga ggttataatg aaatgtgata ttgcttttca tataaaactg aaatggaacc
    28801 aaggtgccgg gaaaaggcaa aggaacagta ttgaataccc ccgtctttag ggattgaata
    28861 accacaccat tttttaccag taaaaaaata tttccttgtt tagaagtaaa gggcgaagga
    28921 aaatacactt cgaatgatgc agagtccgat tcgattcgca ctgtgtcatt tcgccaataa
    28981 ttgttgtatg tgattgtatc atcttctgtt tcgaaacgca tgagaatttt taataataca
    29041 cccatagtat ccatgcctgt attgtaatca tgaaccttaa atacaacggg atcgcttgtt
    29101 cctgcttttt gccgaatgct ttttgctttg cccttcgatg tagaaccaaa atcgggaacc
    29161 cattccaatg ttccattcat aagcttacga taatcacgca catctacccc actccaacta
    29221 aatgctgatg tggcagtatt taaatcgtta ttaatagcct gagttaatcc aaaagattgt
    29281 atcgttcctt tcaccgaagg ctttcctaaa gaatcgacct tgatacatgt gtaagatttt
    29341 gccggagaat tgcatgccag tgcgcatgcc gaatcgagat acaaagcgcc aatcaaaggt
    29401 actgttctta ccaaagtatc cactttagtt ttatttccat aaaaacaatt tagtggcaaa
    29461 tctttatcaa tagtatcggt acaaaatgct tcgaaagtag agggctgccc ggttggtttt
    29521 actttaaagt tttcgcatgc aatgattggc tcacgaacaa atgcatgatt ataacttata
    29581 gcgaaagttt tggcagtgat tggcgaatcg ggccaaagag taaaactgtg ttttatttgg
    29641 taagtgagga ccttgtcact ttgagcagga gtgattgttt ttaaaagtgt gagcggattt
    29701 atggcgcatt ggctttttaa caacaatggc atcaaaacaa aaaacaaact tacatagcta
    29761 aatatttttg aaggtttcat tttgttattt tttatggaga ttaataattc aaatggcatc
    29821 tcaaatataa aatttttttt gattgtggtg atgaatttta aaaataaatc aaatattatt
    29881 tcgaagtaaa aaacaatccc gcgagattaa attcccgcgg gatgttccgg catcatcgat
    29941 tccgtattta gtgtgacaca atccccttaa ttatgtacac ttaatttcat tctgtgtgtt
    30001 tgcccttgca actgtaattc taatgtataa ataccgtttg aaaggtgcat tacatttagt
    30061 acaatatcag tgccattatt aagtaaattt ttctggctaa gaactttatt gcccaatgca
    30121 tcaaacaaat taatatgaac actttcattt gcaaaaccat cgatatgtag tataacaaat
    30181 tcctttgccg gatttggata taaggtcaaa cctatttcgg ttgatgaaaa gtctgtatta
    30241 cccaacttca tttgcggcat tatgaaagtc tgcatttgca tacagcctgc attatccatt
    30301 acctgtaaat gtacaatttg tccggggaac aagcctgtag ccaaaggtcc tgtttgtggt
    30361 ggcattgtat tccagaatat ttgataagga ggcaaaccac caagcacatt tgcaaatgcc
    30421 tgacttggag gcattggctg cacattaatc tgcattggtg gcggactgct aatgttgatt
    30481 gtactattgg ccacacacgc ttttgcatct gttacaacta cgttataagt tcctgctgtg
    30541 aggtttgtta ctgcagccgt tgtcatgcca ttggtccata aaaatgtata tggttttgtt
    30601 cctcctgttc caactattga tgcactaccg ttactacctc caaagcaagt tacacttgtc
    30661 aatgttggaa ctatttgcaa tgggagaggt tgcttcacct gataactgct aactacgctg
    30721 catccattag catcgctaac tgtagcagta tacgtaccag cttttaaacc tgttcttgtt
    30781 tgatttgtag tgccgttatt ccatttgtag cggtaaggaa atgttcctcc tgaaggtata
    30841 agtgatatac taccactaaa atcattattg caatttaatt ttttaacatt ggctgttaca
    30901 gataacagag aaggctcagt taaattgaca gttccagtcc atgaaaaacc acttgcattg
    30961 gttacattta ccgtaaggtt gccggcagaa agattattga taatagcagt agtagcaccg
    31021 ttgctccata cgtaatttaa tggtagcata ttgtctttta cattgtccat taccatagcg
    31081 aacccattga gacttccact gcaccaaggt ccagctgata ctaatgtcag ttcgggattt
    31141 ggaattacaa ccgatgggct cacaggcact tcaaatactt tcgtcatgca tccatttgca
    31201 tccgatactg taactgtata cgttccggaa gaaagattag atatatcttc ggtagtactt
    31261 ccattcgacc ataagaacga tggaagaagc atgtcgcctg ttaaagtaaa atctatacta
    31321 ccattgaatt cagtaagcga acttgccgga gtaatggttg cagtaccatc gtaagaaata
    31381 gtagtgccgg gtacagaaac attcactgta gtataaggaa atgtatcggt agcactgtat
    31441 ggcaataatg tattgatatg aaaactaaac tgtacaggtg ctcccggtaa aattggagaa
    31501 tagaaattta acgtgcccgt tgggaaatca aaataggatg gcaatttgct cgatgcccca
    31561 tctacaataa ttccagggtg gggcgaatta aatactaact gagaaatagg tgagcacgac
    31621 tcgttaatgg ctaccaaact tccactacgg ctggtcaaac caaaagcact gttatcatta
    31681 tttaaataaa atttcatagt accacctgtg catgccaact gataaatagt atcatctgaa
    31741 tcgtcagtaa tttctatact ataaccatct atctttttac tgcaataaac tgtaaccacc
    31801 attttattgc cgggtaaaat acctccacca ttgggtgcgt atacatgtac agtatcgttc
    31861 tttttgccgg gaggttttgt atcccatttt acaccgttca ctaaaaccga atcaatactt
    31921 tctttcgcaa tgatttccaa atcacaaata gtatctcttg tgtcattttt taaaacagtt
    31981 gacgatttgc tgctgctgaa tgtactgccc ggagtagaac cgctaaactt tatctgacgg
    32041 gttgctttat ttcccggatt aatattttgt atgccacaac taatgtcggc attcaggcta
    32101 tcggtaggag tgtatacata cgtagtacaa gctttattaa attttacaga aatggaatag
    32161 ggtgctccat cggcaataca cggaaaaaat acatgcacct cattttttag agttgggcgt
    32221 gctgtaattt ttttgccatc ggttccatat atgctgtcca ctttcttatc atttacccaa
    32281 acctcatcta ttgttactcc tgttggtgct ttaatgcgca tatcgcatac atctgtgccc
    32341 gttgcattac tattggacaa acgtggaaaa ctacttggtt caaaagtagg cgattgttgc
    32401 cctccggtag tatttgtttt gcgagcccgc tttccctgtt ctactgcacc gttattcggc
    32461 agactcctgt ttgccataac atctgcgtta aggctatctg taggtgttat tttgtaggtt
    32521 gttatttcgc tttgtgctgc tactgaaact ttgaaactat caccgtcctt tatgcacttg
    32581 aaatatacat gcactacctt tgaatttgcc gaagtaccgt tgggaacttt agtgctacca
    32641 acttttatac tatctacttt tttattattt acaaacacag agtcgatttt ttgaggactc
    32701 tctattcgca aatcgcaaat gtcggtgcct gtaagattgg tatagccgag tgaagggaat
    32761 ttactggaag caaaagttgg tgactgtgag ccaccaggca ctgtggtttt ttctattttt
    32821 ttatttactg ttgttgcatt gggatttact gcatcattca ccaccaaatc ggaatgcatg
    32881 ctatccgtag gaattaaata aagcaatgat atatttcctt caaactctgc ttgaatggta
    32941 atttgttttg tattgtttac gcaagggtta aatactacat gttgtttgcc atttgctttt
    33001 gctgtcaaag caacagctac gccatttact gttaccgatt ttgccttcac agcaccggtc
    33061 cacaataacc aaaaatcgca aaggtcttca cctaccatat ttacggtttg cagttttact
    33121 tttgccaaag gacccgaatc aacgagcaac ttccctcctt tgataatttt gtcgtcatcg
    33181 gcttttgcgc aatgtagtgt ggtaaaaaac aaaacaaata caattgcgat ttttttgaaa
    33241 acgtgaacta aatgttgttt acgtaaatgg cttaaaaaat gaaataaatt gtagaagcct
    33301 tgttcggtaa cactggtgat gcttgcttta accagcgatt gttgtgaaac aatgtgtgta
    33361 gaatttttca tcttttgttt gattttaaaa tttacgttta gtacttttta ttaatatttg
    33421 aatcaaacat acattgtgat aaaacccttg gaaaggggtt tcttgaattt gacggttcta
    33481 agtttgaatt tgaagggttt gaggttgagg ataaaaacca aaggcaaagt aatactggct
    33541 tcgcagcatt tatatgctaa taaaaaataa aatatgcatg tactaaattc aaagggaaag
    33601 ctttggatta tgcggaataa aatttaatac tattattaag gcaagctaat aaaatcaaac
    33661 ctcaccaagc ttttttaaaa ataaatcctt acgggttcgc gatacatcaa gtgtggcacc
    33721 attcgacatt accacttgtc cgccttcgcc acgaatatat ttttttactt tgtgtaagtt
    33781 tatcaggtaa ctattgtgaa tacgaaaaaa tgcataattg cttaactcct tttcataatc
    33841 ctttatggac tttgatgtaa caatttcagt tttattatcg aagtaaaaag tagtgtaaac
    33901 gttttccgat ttacagtaaa ctattgcatc aatttcaaaa aattctattc cacttttatc
    33961 actaaggcca attttctttt tcgttgtgcc ctcatttata ttgtgcaata aattttcgat
    34021 ttgctttgtg gattggtttt gtaaattttt ttgccgatac ctttctatca catcgcataa
    34081 gtcatcatgg ccataaggtt tcaataaaaa atcgaaagcc gaaaaacgta tagcatctat
    34141 ggcataagaa tcgaaagagg ttgtaaacac tacttcataa tttttattcg ttacagcttt
    34201 caacaaatcg aagcccgtgt gtggcggcat ttgtacatct aaaaacaata tatcaaaatc
    34261 cgaactatcc accagtttaa taccttcaac tacggatgct gattcaccaa taatttctac
    34321 acctggcaaa tgtactgcaa tggcatcgcg caagaaatcg cggtgttgtt tttcgtcatc
    34381 tatcacaata ccttttatca tgcagctaaa ttagtaaaag gaagcaacaa tgtaactttg
    34441 gtgcctgtca catttccatt aacatcaaca atattttcgg ctgtataatc cgatttcgaa
    34501 ttacttagtg cctgcataca atcaagccgt tctttcgtga gggctatacc ataggacttt
    34561 cgtgccgata tattttcctg agttttgcga ccaccaccat catcagttat ttcgatacgt
    34621 attttattca aaattcgcga aatatttatt gcaattgttc ccgaaccacc ttgcgatttt
    34681 atgccatgta ctattgcatt ctcgactatg ggctgcaata gtaaaggcgg cagaaatgtt
    34741 tcattcgtat caatttcatt ttcgaccgtt acattaaaaa ttaagttgcc ttcgagcctg
    34801 tttttttcca gtgcaacata atcatttaag gtttcaagtt catcttcgag tgttacttgc
    34861 ttactcatgc tgtggtcaag tacctttcgt attagtttgg caaagcgcaa taaggttaca
    34921 ctggcattat gcaaatcctt ttttgcaatt aacgtttgta tcgattgcat gcagttgaaa
    34981 ataaaatgtg gattcatctg cgcattgata gcacgcatat tcaggtcagc caattgatgt
    35041 ttcatttgtg cttcgcgttt gcgcctgtaa aaaataaatg caaatactgc aaacgagaaa
    35101 ataaggatgg ccgaatatat aattgtattg cgcaatgttt tttgcttttg caattcctta
    35161 tttgcaagtt cattcttttg ttgttgttgt aactttaaaa tggcttcgcg cttttcgaaa
    35221 tcatattgca attgcttctt catcaattgc ttgcgggtgt catcgttgcg aatgctatcg
    35281 gtaagatttt taaacaatac ctgcatgtta aaagcttttt cgaaacgtcc tgttgccgag
    35341 tataatctgc taagccaatc ggtggcaccc aacatgcccg aagtatattt caaagcaacc
    35401 gattgctgcc atgcatcggt aaaattcttt tccgcctcgc tccacttttt ctgcttcatc
    35461 tgtatgttac caaaatttat tttcgataaa cataaaccta tatcatcgcc aatacttttt
    35521 tgtatatcgt agctggcttt ataatattca atggcttttt cattatcatt caatttatcg
    35581 tatacaccac caatgttgct tagcacaagg ccctgatttt cgatatcttc ggtttgctta
    35641 tgcaatgcta aactttgttc caaataattt aatgcttcct tatgaaggcc atgattgttg
    35701 taaatagtac caagattatt taatgcaacc gccattcctg ttttgtcttt taattctgtc
    35761 attatcgaaa gacattgttt aatatttaca agagcctgtt gccaatctcc ctgttcgtca
    35821 tatactgttc ctatatttcc gagtgaattt gcaatgccat atttatcgtt gctcgcctcg
    35881 taatatttca atccttcgag gtagaaattt aatgcctgtg gcatgtcgcc attttgctgg
    35941 tgcacaaaac ctatattgtt acaatatgct gcataatgaa tggtgccaat gcgatttatc
    36001 gcgccattgt tttgcttcat catccgttgt gctatcattc ccatttcatc gttaagcggt
    36061 tgccatatag tatcctcttc aattaaatca atcaaagtat taatcaacac aacccgctgc
    36121 gaatcaatgt ttgtagatgg cagcaatgca agcaatgaat ctttatttgt ttgtgaaaaa
    36181 gatatggaac taaaaaagaa taaaatcaaa aagcacagta aatatatatt tttcattttt
    36241 agcattttca caatatcaat taaaaaagat agatggatat tctattgtca aagattaatt
    36301 tggctataca aatttaataa aaaaggttaa tcgccaccat gctttgtaag gaagctttca
    36361 acaatgtcct tgtcaatctt ttcgcattgg cttttcaaat actcagaacc tataaattga
    36421 aaccagtatt gaaaaagtta aacagaaaag aaacgaagaa atatttcaac aaatatttta
    36481 atttcgcgct ataaataatt ctaaaattta ttttaatcaa acatgaaaaa actaatcatc
    36541 gcatcaatct tattcttaaa catttgtaat gcctttgcac aaacatttat aaaaataata
    36601 cccaacaata tcccttttga ttttgtgtac tcgggcattt acacactgca agcgcctcag
    36661 ggcgaataca taatggccac aactgttaat gatagtgccg gtagtaacat catgattatt
    36721 aaactcaata gtgctggcga aataattttg cacaacgaac ttttacaacc tctgtttatt
    36781 gaaactgcaa ccaatattat gattacacag gcaaatgaat ttgtaattgg tggagcatac
    36841 aatgtgccta atacaaatta cgatgatgaa ttgctgatgg caaaaacaga tttgaaaggg
    36901 aatcagattt ttatgaaaaa cctcacctca tcgggtatat gttgttggga ctttgctaca
    36961 ggaaatattt tcgaaacagc gaatcataat tatattgttg gtggcgaagg ctttggcatt
    37021 tcttctataa atggatatgc aaaacaaacc gatagtgccg gcaacaatat gcaaactttc
    37081 tctttaggct ccttcacacg caccgaagcc atagttgaaa atagtgccgg agaaataata
    37141 atggggggca aagattttca gttaaatcag gcagtgctaa caaaacgcga tgccaataca
    37201 aattttaaat ggacaaaaac ttacaacaaa ggtttaggtg gtacagtaaa gaatacttcc
    37261 atcatggaaa taatacaact acctacaggc tttttacttg cagcaaaata tccaaatcca
    37321 acaacaggca atgttgattt atggatgata gaaaccgacc tcaatggcga cactataaag
    37381 actaacactt ttgctaacga tggaaatatc attggcatga aacctacgta caatggatat
    37441 gcggtggctg tgatagactc aaataatatt ggtggctacg atttcaaaat tatttttctt
    37501 gataataatt acaataaaat ttcgcaacgc aattatggca ccttgaacat tgatgtgctt
    37561 tatagttttg aaaaatgtag tgatggcgga tttgtaatga gcggacttac ttacgataat
    37621 tcttttattt ataatgccct tgttataaaa accgattcgc ttggatgtgt agcacctgcg
    37681 gcaaatttta catggcaaca ggcaagtttg gccaacaatc aatcgggagt agattttacc
    37741 aataccaccc ttgcgggaat aattgatacc aatgcaacat acctttggaa ttttggcgac
    37801 aacacattta gtacgataaa aaatcctcag catatttatg catcgcaagg aacatacatt
    37861 gttacactta ctgtaacaac acaatgtggt gttgattcgg ttcaaatact tgttacagca
    37921 acttgcaccg gcacttacaa ttactttaca catgcatcat cacttttatc ggccaccttc
    37981 aacgaccccg acccgtcagc tacttcatgg aattggaact ttggtgacaa cactttttcg
    38041 gcaataaaaa atccatcgca tacctatgca gccacaggta catattatgt gtgtctcact
    38101 aaatttaata cttgcggtag tttcactatg tgcgattcgg taaaaataga atgccctgca
    38161 cccttgttaa atattggctc cgacaaaact gtatgtcagg gacagaatgt gatacttgat
    38221 gccggaaatc ccggcatgaa ttacctgtgg agcaatggtg ccagtacaaa aactatttct
    38281 gtaaatactt cgggtacgta tattgttaca gttaccaata gctgcaatgc tttttccaat
    38341 gatactgttg ttatcaattt catcactcct cctgttttaa attttggaag cgacactact
    38401 gtttgcatga acgataccat cactttccaa aattctgcac aaccgggctt ttcatatcaa
    38461 tggtttgtaa atagttattt tgtaacttct caggattcac tcacctatgc tttcaatcaa
    38521 attggttcga atgatataaa ggtgattgct acaaatcttg gttgtattga tagtgcccaa
    38581 ataaaagtag acgtgaatcc tatagctatg tgcacaggcg ttccgctcac gtattgcata
    38641 cccacttaca caaacggcac cacaagtggc gattacataa aacgctttgg cgtgaacact
    38701 ttacttaacg taacaggcag tcagggtggt cctgcttaca acgattatac aaatttgaaa
    38761 actgatataa ccggcaatgt aagttatacg tggacattcg aatttagtgt aacaaatgcc
    38821 atgctgtcga aaatgtggat agactttaat atggatggca ccttcgatga acctgcagaa
    38881 agagtttatt tgcaagcggc aaataatgga attaccactg ccaatttttc gatacctaca
    38941 tcgaaatttt ttggtggcct tacacgttta cggttagtgt gtgccgatgt tgctaattat
    39001 aatttgatac aaccatgtgg tgcatacgac catggcgaaa ccgaagacta cacactcaac
    39061 cttgtaaatc cattcagccc tcctatttct aattttatgg caagcgattc cactataaaa
    39121 ttaaacgatg ccgttaattt ttttcacctt gcttcgaata atgcaacagg ttttaactgg
    39181 acatttacag gtgcaactcc atctacaagt accgcggcaa accctgcaaa catagtatat
    39241 cccacatcgg gttgctatca ggtttcgctc actgctacca atgccaatgg caacgatgcc
    39301 gaaaccaaaa tttgctttat tgatgtagca aataatattg gcatgcaaga agtaacatct
    39361 aatgcattgg aaatatatac cggaaataac aatggtgatt tttatatctt caatccaaat
    39421 atggatgcaa caactgtaac agtatacgat tgtatgggga aaattatttt tacagaaaac
    39481 acatctgaac aaaaaataaa cttaagcctt gcaaaccata aagtaggaat atattttatt
    39541 atcagaagct aaacgcacag cacctgtacc aattggagaa tc
//
LOCUS       DAORQA010000009        37164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_232_length_37164_cov_15.427767, whole genome shotgun sequence.
ACCESSION   DAORQA010000009 DAORQA010000000
VERSION     DAORQA010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 37164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37164
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_232_length_37164_cov_15.427767"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            545..2059
                     /locus_tag="PK736_01710"
     CDS             545..2059
                     /locus_tag="PK736_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32139.1"
                     /translation="MSKYCCTFILFLFSNICFTQNQNSIWCFGDSAKIVFDTTGNVSL
                     LTSSIVSRGTATSIADSNGTLLFYTNTRAGQASAYTGQVWAANDSLMYNGDSIAGRGW
                     YKQHVIIPAPSGNGLYYLVNAAVTAPSGLYYGIIDMTQDSGRGAVVQKNVQLKGPNFY
                     SNDGLAAIKHGNGRDWWVIVRDWQYKNDLFYFYRIDSAGIALSHTQSIGDSSRPSFFR
                     IEPSMDGNLISCIDANGEMVLYNFDRCSGMLSNSIYIEHNHVIPNYAPWYWDSELSAN
                     KRYLYVSEIYSDYDTTNYLLRFDLQAPNIAASRDTLYAGENSAVAGLIELAPDGKMYW
                     SCAYNPPNGFNYPYPDSVHNYITDNLSVINYPDSSFCDFQPFSFYLGGKRTYYGLPNN
                     PNYSLGRLLGSPCDTLQWTNLTPALSKGEGVMQITYISDWEKLFVNASGLKGKNVTVS
                     IYDGRGSLKFEVISLKSNAGYFTLDVDCSGWSDGLYVVQLQTDKERLSKKFVKE"
     gene            complement(2063..2554)
                     /locus_tag="PK736_01715"
     CDS             complement(2063..2554)
                     /locus_tag="PK736_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRC32140.1"
                     /translation="MKKAAIFILILFTKCFAGLQAQQVEIMKFDALQQILSSNNDTTY
                     VINFWATWCKPCVHELPAFDTAFTKYANKPVKIILVSLDFKREYETRLVKFVNEHKTL
                     PLVVLLDEPDYNKWIDKVAKEWGGAIPATMVFNNKNHFRYFHEDELTFPDLCKRIDEG
                     LTK"
     gene            complement(2590..3036)
                     /locus_tag="PK736_01720"
     CDS             complement(2590..3036)
                     /locus_tag="PK736_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32141.1"
                     /translation="MRIQLWLVMMLWASFSFAQDGEPATGATTEEEYNYCETGYKLQL
                     QMELPMKKGYVLNNITTVQEGDRTIEFKGLMRKGDKKPCAILMVYSKPRMAPLYFGIP
                     SSDAPSTLWTKYYTSLNSDTENEKDVFRFFSYALGKLTAVLAQYQQ"
     gene            complement(3050..4039)
                     /locus_tag="PK736_01725"
     CDS             complement(3050..4039)
                     /locus_tag="PK736_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRC32142.1"
                     /translation="MKTHYDILGVDNSASVTEIKRAYRQLAKRYHPDMNPNNANAQEL
                     FLHVQAAYEILSDTNKRYAYDKQLSGDYYHTSSPAFDHYFVVSASKTHVKLNDEVFIT
                     FTYSGEGRIFRQPDFRGFHVIGKPFIHHGTSVRNGMTMKETSLTFTLAPIQKGLLQIK
                     PAAIKIFNKQFSTSPIGLFIDDNVCHFSSNKKADGTPLIVPLIFEENAQGHLFTNAVY
                     HRHDVLIPRSKAATHFHWLGKLVKIVTAAWIAMLGLKYGYNFWPTLLVGLCMGGVNTQ
                     LMYKLAGIKSQFRQVRKFPLLQYYFSKGYYPRTILFAERDYNHVIYFVTSLIF"
     gene            complement(4306..5184)
                     /locus_tag="PK736_01730"
     CDS             complement(4306..5184)
                     /locus_tag="PK736_01730"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018471963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRC32143.1"
                     /translation="MIVALYGRQQNVTSVPFIEELVKALHEASVDVWIYESFLNFINR
                     HATTKIELPVFAANEDIVNKVDIFFSIGGDGTILDTIEMVQDSGIPVLGINTGRLGFL
                     SSINKSEIHKAINCLQKGLYSLDPRTLLRLESNKTLFGKVNYALNELTIHKKDSASMM
                     TVHTYLNGEYLNSYWADGLIIATPTGSTGYSMSCGGPIIMPQSENFVITPIAPHNLNV
                     RPIIVSDKSVISLEVQGRSYYFQASLDSRTVTIDSSIQLAARKEDFMLNLVRLDNENF
                     LGTLRNKLNWGLDLRN"
     gene            complement(5194..6444)
                     /locus_tag="PK736_01735"
     CDS             complement(5194..6444)
                     /locus_tag="PK736_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRC32144.1"
                     /translation="MDNKIISKYTARGVSADKEDVHAAIKHVDRGLYPNAFCKIIPDY
                     LGHDPEWCNIMHADGAGTKSSLAYAYWKQTGDISVWKGIAQDAIVMNIDDLLCVGATD
                     NILLSSTIGRNKNLVPGEVIKAIIEGTEEIVAQLNEYGIHINTTGGETADLGDLVRTI
                     IVDSTVTCRLKRSDVIENNIQPGDVIVGFASYGKAIYESEYNGGMGSNGLTSARHDVF
                     EKSLAKSYPETYDTALPDDIIYSGNYFLTDIIYIDKNENYKIMRDEKVLIDDNYNHDL
                     FAVKTTVGKLVLSPTRTYAPLIKNILSSWRQHIHGIVHCSGGGQTKVMHFADNICITK
                     NNLLPVPGLFKVIAHQSQTALHEMYKVFNMGHRMEIYTNKEAATEMIAIAKGLNIDAQ
                     IIGYCSVHKGKKLVIETGNEQLIY"
     gene            6761..9919
                     /locus_tag="PK736_01740"
     CDS             6761..9919
                     /locus_tag="PK736_01740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32145.1"
                     /translation="MKKIFCVYVSLISLIFIDINLQAQSCVSCPTCFTAGSELVTNGN
                     FAFSAPYAYTATAPLVNAVYTALPPVNFGNYSITNNPNTYNSFWCGADHSGGGTMMLM
                     CDGYNGSPTSAWGQYINISTIAPNTNFTFCFYAMNILCPGNLSIDPLIQMYVNGAPLL
                     GAAYTLVENNNQWVLITGTWNSGNTPPANVLLEIATTSYSGSGNDFAIDDISFKACLP
                     SFLTSASNDTAICKGKNVQLLAAGAANYAWAPATGLSSTSIANPVCNVQATTTYTVSI
                     SNGLGCTKTESITVTVNQLPAVNLGSDTSICNGNSLILNAGNYIGYQWQNGNTTATIN
                     VNSTGTYTVTVSDANGCSNSDAIDVTVNPLPVFNATPAIDSTCAGTSVSLSVSNNTYN
                     YTWSPGVGLSATTGSTVIANPPASTTYTVTGTNGFGCTASSTVSLTINALPNAGAGAN
                     KTICKKDSIALNGSGGVQYSWLPAGSLSNANIANPKASPQVTTTYTVTVTNINGCTAT
                     ATMKLKVNALPVVNLGSDTTLCKNIPIVLNGNNNTAYTFLWQNASTNINFTANDSGTY
                     YVQVTDTNNCKNADTVVINYYPSALITLTSSTNKLCIGDSAFVNAGGGVSYNWTPAYA
                     INNTSSANVILYPDSSTTYILTVADSNACQYVDTLAITVFNLPVVNAGADTAFCFGNS
                     VNLLAMGGANYSWLPAAGLSDTAIANPVATPAFTTNYIVHVIDTNGCHNTDSVLLTVH
                     ALPIVNLGNDTTLCTTQQITLATAGTFNSYLWQNGDTNATITVNTSAAYILSVVDSNA
                     CANTDTVDVTFVVCPPPVIGFSCTDTLFCEKQCIDFNDLSTNNPTSWLWTFTGASPIS
                     STLQNPANICYNNYGAFDVQLIACNVGGCDTLTLTQFVQEFQTPAAPVIFQTFDSLIC
                     PTTNVTYAWYNTNNPNNVLSTNYYYIPTVPGNYYVLIADSNGCAVASNIIGSNVGMYD
                     NNNIQGYTIINDGANLIIETNTKQLIQARIFNLAGQVVKQQFFNNSTLKYIVQLPPAS
                     GIYLLELVDSDGGVVHYRGLSK"
     gene            10002..10781
                     /locus_tag="PK736_01745"
     CDS             10002..10781
                     /locus_tag="PK736_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRC32146.1"
                     /translation="MTKVQYCSDLHLEFQENKNYLKLFPIRPEGEILLLAGDICLLKT
                     MKEHDDFFNFCSDNFETTYWIAGNHEYYHSDLSDRIVKSDEFQSMHEKIRENVFLINN
                     KTILHKNVEFLFSTLWSYIPQLYEKIVQRSVSDFHLIKQKGKLITAQDFNEMHKCGFD
                     FLQSELAKPKDKQRIIITHHVPTLMHYPVQYKNSNINFAFATELFEFIETSNADYWIY
                     GHHHCNTPPFQIGTTTMLTNQLGYVRQNEHSQFDCDATIEI"
     gene            complement(10875..12275)
                     /locus_tag="PK736_01750"
     CDS             complement(10875..12275)
                     /locus_tag="PK736_01750"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRC32147.1"
                     /translation="MKKISFLAALIMLQIMAMGQQTIQIEPFDKISISSTANIKLVPS
                     QSYTITATANDISSIFTVNNNVLTVSSPLANVVEVGIKDLTELKVSGNGQVSSDVPIV
                     INDLLINVSGKLTLNLKVTANKISVNTSGVSKGEISGTANEMEFNTSGISKLNAAELK
                     VKKFDANISGSCKTTVDVTDELNTNVSGSATIYYKTKPLKMSANNSGIARVDDVANKS
                     ANDTTRISIGNININVSGENDDDDGSKNVDVKKGLHKDDHIEPHWGGLELGFNGYVPG
                     IDALDRANDGPANYDFLQLNSGKSIAVNLNLIEIKAKLIKRYLWFNTGAGFMWNNYRF
                     DNNNRVLIAKTDTVQALEISKDLSKNKLTVSYITIPAMFEICTNPLRKKAIHLGFGVV
                     GAYKLGAHTKYKTSDGVNKTRDDFNINPFRLDGRVCIGFRNYNIFAQYGINELFKQNS
                     GPALHPFTIGLSLVSW"
     gene            complement(12368..13006)
                     /locus_tag="PK736_01755"
     CDS             complement(12368..13006)
                     /locus_tag="PK736_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC32148.1"
                     /translation="MHIYVISGLGADEKVFCHTYFAQHTVVHLPWLPVITSESLQQYA
                     MRMAATIDTGIPFMLMGLSFGGMVAIEIAKVYKPQKIILLSTIKTQYEKPTYMKALQK
                     IPAHKYFPLTWMQKMKTTASFMFGVTSSDEQNLINTYVTNIDIAYLKWSIDKIIHWQN
                     ETYPGNVIHIHGSSDRIFPIQNIKADFVIKNGGHFMLMNKAGEVNEILRKIL"
     gene            complement(13393..13953)
                     /gene="frr"
                     /locus_tag="PK736_01760"
     CDS             complement(13393..13953)
                     /gene="frr"
                     /locus_tag="PK736_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRC32149.1"
                     /translation="MEEVKKIFDHAVGEMNNALDHLETELSKIRAGKASPQMLDGLYV
                     DYYGANTPLNQVGTVNTTDARTLVVQPWEKSMLVPIEKAIQAANLGLNPQNDGIVIRI
                     AVPPLTEERRKDLVKKTKAEGEHCKVTIRNARRDANEGLKKALKAGLPEDVEKEATLK
                     VQHLTDSYIIKVDKHLETKEKEIMTV"
     gene            complement(14208..14915)
                     /gene="pyrH"
                     /locus_tag="PK736_01765"
     CDS             complement(14208..14915)
                     /gene="pyrH"
                     /locus_tag="PK736_01765"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRC32150.1"
                     /translation="MKYKRILLKLSGEALMGNKQFGIDTERISQYAQDIKDVQAAGVE
                     IAIVIGGGNIFRGLQASESGMDRVQGDYMGMLATVINSMALQSALEQIEVNTRLQSAI
                     KMEAICEPFIRRRAVRHLEKGRVVIFGAGTGNPYFTTDTAASLRAIEIEADVILKGTR
                     VDGIYTADPEKDATATKFDSVTFKEVYDRDLKVMDMTAFTLCEENKLPIIVFDMNKKG
                     NLLNLVQGEKIGTLVTI"
     gene            15089..16381
                     /locus_tag="PK736_01770"
     CDS             15089..16381
                     /locus_tag="PK736_01770"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HRC32151.1"
                     /translation="MKKSVLFYFGLIATLLWGACNKADEIGNGILPADDIPGLLFSDT
                     ASIVSFCITDDSIKSGGVTNKTFLTSVNPVGAQNDATFGATVAGLYAQVDLSKTSFDF
                     GTNFIADSLVLTMAYKEVFGDTNSPVTFHVYRLNDKISSDSIYFTSKDYFTYNAQEIG
                     SATVVLRPKDSITEYDVKRAPHIRINLDPALTVEFASKTQSNELKINDDFLNFFKGIY
                     ITADVPSAAGSGFIFTLDYKSVASRMALFYHNDAGDSAVFAFPFNDYNRVNRFKHDYS
                     GTAIATQLVDSTTGGDYSYLQSLGGLQTKIKLPNLKDYNLINKRAINKAQLIIQADNS
                     EVTKYAAHKTISVFTANGSNQITDKFRILPMAYDSVKSQYIADITVYAQQALTGKYDD
                     LGLILKANGALVNAYRTRLFGNSPIDKRMKLKLYFTEY"
     gene            16461..18800
                     /locus_tag="PK736_01775"
     CDS             16461..18800
                     /locus_tag="PK736_01775"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRC32152.1"
                     /translation="MVKTLLTGTLLLITLTCFGQQKIGQWRVHLSYNQTKAITVSPTK
                     IYVGANDAVFYYDKENNEIKTLSKVEGLSSIGINTLKYNDYNKCLLIAYSDANIDLLK
                     GKTIINISDIKRKNIIGGKNINNIHFKNEFAYVSCSFGIVVINTDRNEVKETYTLGST
                     NSNIKIWSCTTDDNYIYAASDSGVYRATLTDPFIVNPTAWQKVFAIKPGSGGVKYITN
                     FNNKIFANQVQTTTLDSLYIMDIATQQWSGFAIGANETQTKLSMQSSGDVLTLTSLYF
                     VHSIDKNFNVTTYNNTNIAFTTANDAAENNGNIWIADANKGLIKYTPQQGSEIIVPSG
                     PRSNKVALIDGSENNIWIGHGPRFNNWAPAGVVDGFAKFDNTQWTTFHGNAPGNSFSL
                     DTVEDLICIAADPSNKNHVYFGSWGRGLVEYDNGTIKLYNDANSPIKVQQFIANYRHL
                     RIGGLALDQNNNLWITNTLSDSGLIVKKNDGTWKKFYVPLLSGGDYVTDIIADDYNQL
                     WINVPISGANNTGILVYNHNGTIDNVSDDRVRLLSDEEGKGQLPSKAVRCMARDQDGL
                     IWVGTEKGVGVFYSPSSVFSGGNYDAQQILIVQETYNLYLLDKEVVQAINIDGANRKW
                     FGTQSGGAFLTSPDGVNQVLNFNETNSPLLSNNIYSIKVNDATGEVFFSTDRGLISYQ
                     GDATQGSAACENVLVYPNPVRENYEGPIAIRGLVNNGNVKITDVSGNLVFETQSLGGQ
                     AIWYGKNLKGEKVYTGVYLLFCSDADGTNTCITKLLFVR"
     gene            18832..19557
                     /gene="recO"
                     /locus_tag="PK736_01780"
     CDS             18832..19557
                     /gene="recO"
                     /locus_tag="PK736_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRC32153.1"
                     /translation="MQISTQGIVLQTVNYSDTAVIAKIFTLHHGLVSFMVKGVRKKNA
                     RFAANLFSPCSILSMNYLFKVNKNLQFVNEISCDVPLTNLQSNPLKSCIAFFMAEVCV
                     KSIKEQEQDELLFDCIRHFIELLNDTEDSIKQFHHYFLLQLANKLGFGPQGIYNDMHT
                     VFDLREGIFQSSYPVYPEFVINRTAELISLFTNSTFENSISITASKAERNSLLATLLF
                     YFDLHVLHGSKIKSTDVLSEMMA"
     gene            complement(19562..20164)
                     /locus_tag="PK736_01785"
     CDS             complement(19562..20164)
                     /locus_tag="PK736_01785"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRC32154.1"
                     /translation="MKKVFRSLFTFFMQGALITVPVALTIIIFFNAIRWIDGLLPFHI
                     PIKISDGYTFELPGLGILSIMLGVTILGYITTRFVRNPVLYYIDNFFESTPLLKIVYT
                     SIKDLIAAFVGEKKRFNHPVLVMVDKEAQVQRIGFVTQDDLGFLGIPQGKVAVYLPFS
                     YGFNGQLVIVPVANISPINASGTEMMKFIISGGVTDVENN"
     gene            complement(20243..20599)
                     /locus_tag="PK736_01790"
     CDS             complement(20243..20599)
                     /locus_tag="PK736_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRC32155.1"
                     /translation="MNIELIRNYCMNKKGVTESFPFDSDTLVFKVMGKMFLLTSLSEP
                     EKINVKCDPETAIDLREKYDAVQPGYHMNKKHWNTIDMAQGLTDVLIKKWIDDSYDLI
                     VNDLPKKVKLEYEGLK"
     gene            complement(20596..21882)
                     /locus_tag="PK736_01795"
     CDS             complement(20596..21882)
                     /locus_tag="PK736_01795"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HRC32156.1"
                     /translation="MHEHDYIHQVIEPIIIYYKKLDVNNRIKFVQRVQYFASNFEFAG
                     TDGFEMTDEVKYLLCCPLTQITFGLEEFDMHNFSRIIVTPGVFYAPRSQLYMKGGTFK
                     SGLVMISWDNFYEGYLHPDDNYNLGLHEFAHVYKLCIDTTKQFDMHLQNLTQEWEKVG
                     NEIFLKMQNSGAESFLREYAGTNPHEFFAVCVEYFFEVPQKLMQNYPDLYDALCNLLN
                     QNPINSRGNYYHHNAIGRQQEVQTKPKNISLEARNFEPSNFVFPFLLSGFFAVCGWVP
                     FMYLLDHQLISITNTILIVVALGLSFYYFINARMGYQSISNKFNLAFVCITVLGMPLM
                     MLALFLNSNITLNEPKSIQLEVMKVTINTPPDPDKWRAIVEYKKGLFDNYYFVRRMKY
                     DDAWKQWKEVVYKVDKGCFGIYVVKEIKFIKHTIEE"
     gene            complement(21895..23136)
                     /locus_tag="PK736_01800"
     CDS             complement(21895..23136)
                     /locus_tag="PK736_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRC32157.1"
                     /translation="MKSIYTFYFAVLCYTTNIVAQDINYAKSVIETLTSKKYAGRGYV
                     KNGEHVAATFISKEFLKFELKPFGRSYNQDFSFPVNTFPGKMEIVADGVSLIPGIDFI
                     VDPACPAITGTFEIVELLTVDDWYKKSKLDNKIVLIDLSLFSDSLKKVVRAYINENRS
                     NIGFIYLEEKKLTWSVSTSQKNKFSVSVLKQSFNKNSKTLKVNIDALILPDHKSQNVI
                     GFIEGEVKDSFVVYTGHYDHLGMMGKKTIFPGANDNASGISFLLNLAKHYSKHKPKYT
                     VVFIAFAGEEAGLIGSRYYTENPLFELYRIKFLLNFDLMGTGDEGIMVVNATEFSEAY
                     TAMVTINNDNNLLTKVDKRGKAANSDHYWFTEKGVPSFFIYTLGGIKAYHDVYDIAKT
                     LPLTEYVDLFKLITLFANQIN"
     gene            complement(23222..>26271)
                     /locus_tag="PK736_01805"
     CDS             complement(23222..>26271)
                     /locus_tag="PK736_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32158.1"
                     /translation="GTNVSCNGGTNGSGTVNATGGSGYTYLWNNGVTTQTRTGLAAGT
                     YTATVTSANGCTTTCSVTITQPTALTCSVASTTAACGVCNGTATVTATGGTPTYTYLW
                     SNAATTASISGLCGGTYTVTTTDSKGCTSTCTTLVGSTGSIACTIAGTNVSCNGGTNG
                     SGTVNATGGSGYTYLWNNGVTTQTRTGLAAGTYTATVTSANGCTTTCSVTITQPAALT
                     CSVASTTATCGVCNGTATVTAAGGTPTYTYLWSNAATTSSISGLCGGTYTVTTTDSKG
                     CTSTCTTLVGSTGSIACTITGTNVSCNGGSNGSGTVNATGGSGYTYLWNNGVTTQTRT
                     GLAAGTYTATVTSANGCTTTCSVTITQPAALTCSVSSTAAACGVCNGTATVTATGGTP
                     TYTYLWSNAATTASISGLCGGTYTVTTTDSKGCTSTCTTLVGSTGSIACTIAGTNVSC
                     NGGTNGSGTVNATGGSGYTYLWNNGVTTQTRTGLAAGTYTATVTSANGCTTTCSVTIT
                     QPAALTTSTSTTPASCGICNGTATATPAGGTAPYTYSWNSIPVKTTATASGLCSGTFV
                     VTVTDSKGCTRTAQAIVGSASSTVTCSITTNNNVSCFGANNGSLTALAGGGTSPYTYL
                     WSNGKTTAINSGVTVGTYTVTITDVNGCTSSCTKVVTQPTQIVVVMSGKDASSCNNND
                     GTATASVSGGTPGYTYVWSNGGTNSTIIGLVAGTYTVTVTDSKGCTKTGSIVILQPAY
                     VCTTFYTGHAQTYWNSNWKPDTLYKYLDANFNTVFPAGLKLQGTCGTAKSLLLTSAAA
                     ARITMQGTVNVAALTQSYVDPTLLALNNSLAAQLVTLALNIQFDLANPNFAPSTVNFK
                     DLVVDSGPLTGLTVQQIFNEGVNALTGCGSAYSNSILRTYAAFINSSWYIGNKSNNVL
                     TCPVNNCSKSSTWFSTDDELSFNAYPNPVLDELAISFISSKDTKFDVVIFDMTGRVVY
                     RQESLATEGANTFRYNTSSFAQGIYTVQLRIDGLTRTVKIVKQ"
     assembly_gap    26272..26371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26372..>37164)
                     /locus_tag="PK736_01810"
     CDS             complement(<26372..>37164)
                     /locus_tag="PK736_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRC32159.1"
                     /translation="DLTTGCTASCQSVLVVNQSTPAVTCQVIQNNTHCFIFNGQCTVN
                     NNLVSPTYIWSNGQTTQSLSGLAPGVYTVTVTEMLTGCSATCSLEILNTPAAIPTANC
                     TATDNTGCTTQNGSASVTTNALNPTYIWSNGQTTASISGLAGGTYSVTVTDNTNGCTA
                     TCEAIVGTNSTGISATCSSTDVNSCIAPNGTANVITNSLNATFLWSNGNTTNAISGLS
                     AGMYTVTVTDALSGCIGTCATTVNDAVIPVTATCGSTDRTNCSVPDGTVSVLTNAVSA
                     DFLWSNGDTNSSQNNLTAGTYTVVVTDINSGCSQTCSATVADDFAIISLSLVTIDSVS
                     CFGFADGSIEVTGLTNGNSVGPYEYSLDNSSWQSSGLFTGLTAGVYTVYVNDLLEQCT
                     YQEDYTIYEPDYLTISNELVNNINCWGENSGSVDITVVGGTLPYIFAWSDGTTNEDLV
                     NVVADVYTVTITDANGCEFVSAFDVLEQPRYAIGLNNCCTSVSCHGATDGTAFVQVNG
                     ATPFYYGYNYLWNTVPAQTTALATNLPAGIWSLTITDSLGCDTTISILVKEPTLLVAS
                     TNVSAITCNGANNGIITLSVIGGEPFTVGDAYNIVWNTIPPQTGFQATGLGGGTYTAT
                     IIDSLNCIKNVIVTLTEPDPIFISMIGTDVSCFGASDGEVTADPVGGVGPYTYLWSDG
                     QTTQTASNLSANTYTVTVTDFNGCTSSASILLSSPSQINAVYTVVNPTCPASTDGKIL
                     QTSISGGLAPFSYLWNSSPTQTTANLLNVAGGTYTVIVFDANGCSAQFTYSVLNPLPL
                     ACNATVVPITCSGNNNGSITLNTSGGTSPYTYLWSNGNMTSTNSGLALGTYTVTVTDV
                     SGCTNSCSYTVSQLPGLSVNLSATNVTCNGSSNGTIISSIGGGLAPFGYLWSNGSTTI
                     NLTNIGPGIYTLTVTDNNGCSGTASVSITQPSALSCICNASVINVSCFGGNNGSVTAL
                     PIGGLAPYSYVWSNGQTTQTIIGLTAGTYTVTITDANGCLKVGSVTITQPNQLFALMG
                     CVQNVTCNGGSNGTACVNGFGGVAPYTYSWNTVPTTTTSLATGLMAGTYTGIVTDANG
                     CTASAQITISQPQLGVSCSITNTNITCFGNTNGSASAAVNGGTAPYTYLWSNGKTTQT
                     VINLATGVYTVSVTDNVGCSSTCSVTITQASAIAALYTVVRPLCNGNTNGSIDVSVVG
                     GTSPYTYGWSNGATTQDLNNIGAGNYVLTIIDSKGCVLNASIAVTQPAVLNCSITKTN
                     VTCNGGSNGSATVTATGGTSPYIYLWSNGVSTANNAGVAAGLYTVTITDSKGCTKNCF
                     VTITQPSKLVCNANVLNIATCNNNNGKAQAVASGGVAPYTYIWLTAPVQTTAIATGLS
                     SGTYVVVISDSKGCSSSCSVTVPSSNALSCSVFGINVSCNGGNDGTATVTVNGGVAPY
                     TYLWNTSTAATTSSINSVNAGTYTVIVTDANGCTTSCFIVITQPALLTCVAISTSLSC
                     DGSANGTATASVTGGTSPYAYLWSDGQTNITATGLSSGSYTVLITDSKGCTSQCATTI
                     AVVSPLSCQTSVTNATCTGFPNGTATVTTSGGLAAFSYVWSTTPPQFGATATGLSAGL
                     YTVTVTDANGCITTCDASITQPGNLSCIAANTMPVTCYAGNDGEAQVAVNGGTPGYTY
                     LWNTTPLQTTSTATGLSGGSYIVIVTDNVGCTSSCNVLVVQPAVLACSISPINTTCGL
                     NNGSATVNATGGTSGYTYLWSNGQTNATISALATGNYTATVTDANGCTTSCNTGINAS
                     SSIILTINKTPTNCNSICTGTASVSGVAGGTSPYTYLWSNGVTSVSATGLCAGTYTVT
                     VTDNIGCSGSFSFNINQLPSLIVNISNFSNVSCFGLADGSAQVVASGGSTPYLYLWSN
                     GQTNALASNLSAGNYTVTVTDKKGCTGTAVVTITEPAQLFASETLVNVTCNGGTNGSI
                     DVSLIGGTPPFTYSWSNGQTTQDINNVPANTYIVTITQSNGCSQTLSVQITQPNALIC
                     GINSSSPSCNGNSNGTANVVVTGGTPGYTYLWSNGISGSTLSGLIAGTYTVTVNDANG
                     CTTVCQTTITDPAVLMLTSGKADVKCKGGSDGVASVVASGGTSPYTYLWNNGQTTSSI
                     SGLPANSYTVTATDNNGCSASLVIIINEPSQLQLITTGSNPTCGNTNGSASASTAGGT
                     PSYTYLWSNGQNGAIISAIGSGTYTVTVTDANGCTATSSVTIGLSSNLNLNIVPTSPL
                     CYGSAGSAIANVTGGSGIYSYIWNSVPVQTNLIATGLNAGSYTVIVSDNAGCTVSGSV
                     TITEPQLLECSTTATGAACNGGCNGTATVAATGGTSPYVYNWNTIPTQTNATATGLCA
                     GTYTVIVTDANGCTTSCVVSITGSPLLTCSVFKSNATKCGLPTGKLKAIPLGGSSPYT
                     YLWNTGTTTQVLNFLFGGTYTVTVTDKFGCTSSCEATLKAHGPVDCFVTSTDASCGAC
                     NGTASVLASGGGLAYTYKWSTGATTKAISGLCPNTYVVTVSDQYACTKTCVVIIVTNN
                     NLSCSVTGVNVQCNGGSNATATVSAVGGTGIYTYLWTTGATTQTVTGLTAGTYTATVT
                     SGNNCTSTCSIVITKPQPLICTITATDASCGVCNGTGIINVVGGTAPYTYLWSNGLTN
                     NVATTLCAGSYSALITDANGCSSACSVVINSIGNISCTISGTNASCNGLNNGTATVNA
                     SGATATSYLWSNGQTNQTATGLVAGAYTATVSAGPGCTSSCVVIITEPAAITCTTTST
                     LASCGVCNGSASVTAISGNPPFTYLWNNGSTTTSLSGLCGGLYTITVTDATGCTSICN
                     VSVGTSNPLSCIISRTNVSCNGGTNGSATVNPTGGAGYTYLWSNGSTTKTITGLGAGS
                     YTVTVSANNGCTTSCNVTITQPAILSCATTSTPAKCGVCNGTATITATGGTIPYTYLW
                     STGSTTKTILALCSGVYTVSVTDKKGCTTTCTVSVGATTPISCSMTKKDISCNGGNDG
                     TATVTAFGGTSYTYLWNNGQTTATATGLTLGNYDVTVTEANGCTSVCTINITEPAALT
                     CSALTTNASCGQCIGTATVSSLGGTAPYTYLWYNGSTATTISGLCAGSYSVTVTDSKG
                     CTSNCVAVVSGAGTMSCTTTKTNITCNNGSNGTATVIPTGGANYTYLWSNAATTSTIT
                     GLSSGTYTVTVTAGICTTSCKVVLSNPNLLTCSIFATNVACGCTGSATITPVGGVAPY
                     TYLWNNGQTTKTRNGLCAGVYTATTTDSRGCTTTCEATISGGTGLSCSINGTNVSCNG
                     GSNGIATVTAIGGSGYTYLWTNGSTNQTNTGLIAGTYTVTVTSSGGCTTTCSLTITQP
                     TALSCNATSTNASCGVCNATANVTSNGGTLPYSYLWSNGATLTSLTALCSGTYTVTTT
                     DGKGCTATCTTIVTAVGAISCTITGTNVSCNGGTNGSGTVNATGGSGYTYLWNNGVTT
                     QTRTGLAAGTYTATVTSANGCTTTCSVTITQPTALTCSVASTTAACGVCNGTATVTAT
                     GGTPTYTYLWSNAATTASISGLCGGTYTVTTTDSKGCTSTCTTLVGSTGSIACTIAGT
                     NVSCNGGTNGSGTVNA"
BASE COUNT        11081 a         7356 c         7043 g        11584 t
ORIGIN      
        1 aaggcacgat gtgttcgaaa aatcattggc caaatcctat cccgaaactt acgaaactgc
       61 gtgcacaatt gaacgatgcc attccaaaac ctgcagcacc tgtagtacca gcaaaaactg
      121 aagaagctga aaaaccttca gaagctgaaa agggagaata attaattttt tagaaacgaa
      181 ctttataaac ggttactgta tacgaatgca gtagccgttt tttttgcttg aaagtttttt
      241 taaattcgga aaggctttcg gtaaaaaata attttggata tagctgtacg aaaaacatta
      301 taacaagctt gcgcctgatt atcatatcac attagcttac aaaaaattaa tttccatttt
      361 tttacacggt tgaattttta caattctact ttcttgatac aaaaatgatt ttctgcacag
      421 gttttctttt agaaaaattt tcttgtacca ttcgttttcc tttatacatt tacaacacaa
      481 aatatatttg gctttgaact tacagttgaa attttattta cacatcaatt aatcagtttt
      541 actcatgagt aaatattgtt gcacatttat tctcttttta ttcagcaata tttgttttac
      601 tcaaaaccaa aattctattt ggtgttttgg cgatagcgca aaaattgttt tcgatacaac
      661 aggaaatgta tctttactta cgagttcaat tgtgtctcgt ggtacagcca cctctattgc
      721 agatagtaat ggaacacttt tgttttatac aaatactaga gctggtcagg catcagccta
      781 tactggtcaa gtatgggctg caaatgacag cctcatgtac aatggcgatt caattgcagg
      841 cagaggctgg tataagcaac atgtaattat tcctgcacca tccggtaatg gattgtacta
      901 cttagtaaat gcagcagtca cagcaccgag tggattgtat tacggtatta tcgatatgac
      961 ccaagacagt ggtcgtggag ccgttgtaca aaaaaacgtt cagctaaaag gacccaattt
     1021 ttacagtaac gatggattag ccgctataaa acatggtaat ggtagggact ggtgggtaat
     1081 agtgcgtgat tggcaatata agaacgactt gttttatttt tatcgcatag attctgccgg
     1141 tattgcatta tcgcatacac aaagcattgg tgatagttct cgtccaagtt ttttcagaat
     1201 agaaccatct atggatggca atttgatttc ttgtattgat gcgaatggtg aaatggttct
     1261 ttacaatttt gaccggtgct caggtatgct ctccaactca atttacatag agcataatca
     1321 tgtaattcct aattatgcac catggtattg ggatagcgag ctatcagcaa acaagcgtta
     1381 tttatatgtg agcgaaattt acagtgacta tgatactaca aattatttac tccggttcga
     1441 tttgcaagca cccaatattg ctgcaagcag agataccttg tatgcaggtg aaaatagtgc
     1501 tgtggcgggt ctcatagaac ttgcccctga tggtaaaatg tactggtcat gtgcttacaa
     1561 tcctccaaac ggatttaatt atccttatcc cgatagcgta cacaattaca taacagataa
     1621 tttaagtgtt atcaattatc ccgatagcag tttttgcgat tttcaaccat tctcgtttta
     1681 ccttggtggt aagcgcactt attatggttt accaaataat ccaaattata gtttggggcg
     1741 gttgttgggg agcccttgtg ataccttgca gtggacaaac ctcaccccgg ccctctccaa
     1801 aggagagggg gtaatgcaaa ttacatacat atcggattgg gagaagttgt ttgtaaatgc
     1861 aagtggatta aaagggaaga atgtaacggt gagtatatac gatgggcgag ggagtttgaa
     1921 gtttgaagtt ataagtttga agagtaacgc agggtatttt actttggatg ttgattgtag
     1981 tggttggagt gatgggttgt atgttgtgca actacaaaca gataaagaaa ggttgagtaa
     2041 gaaatttgtt aaagagtaga atttatttcg tcaacccctc atcaatcctc ttgcacaaat
     2101 caggaaaagt aagttcatcc tcgtgaaagt aacggaaatg atttttgtta ttgaatacca
     2161 ttgtagccgg aatggcacct ccccattcct ttgcaacttt atctatccat ttattgtaat
     2221 cgggttcgtc aagtaataca acaagtggaa gggttttatg ttcgtttaca aattttacaa
     2281 gacgggtttc gtattcgcgt ttaaaatcga ggcttaccaa aattattttt acaggtttgt
     2341 ttgcatattt tgtaaatgca gtatcaaacg caggcaactc atgcacacaa ggtttacacc
     2401 atgtagccca aaaatttata acataagtag tgtcgttatt gctgctaagg atttgttgca
     2461 gtgcatcgaa tttcattatt tctacttgtt gtgcctgcaa gcctgcaaag cattttgtaa
     2521 ataatattaa aataaaaatc gctgcttttt tcatgctact attttgaaga atatagaaat
     2581 caaaattcct cattgttgat attgtgccaa cacagccgtt aacttgccga gtgcatacga
     2641 gaaaaaccgg aacacatctt tttcgttttc ggtatcgctg ttgagcgaag tgtaatattt
     2701 tgtccacagg gtggagggtg catctgacga tggtatacca aaatacaatg gtgccatacg
     2761 cggcttagaa taaaccatca atatggcaca gggtttttta tcgcctttgc gcatcaagcc
     2821 tttaaactca atagtgcgat cgccttcctg cactgtggtg atattgttta atacataacc
     2881 ctttttcatg ggcaattcca tttgcagttg caacttatat ccggtttcac aataattata
     2941 ctcttcttcg gtggtggctc cggtggcggg ttcgccatct tgtgcaaaac tgaacgatgc
     3001 ccaaagcatc attactaacc ataactgtat acgcataaaa tttatcaatt taaaatatta
     3061 acgaagttac aaaataaatt acatggttat aatcgcgctc ggcaaataaa attgtgcggg
     3121 ggtaatatcc tttcgaaaaa taatattgca gcaaaggaaa ttttcgcact tgcctgaatt
     3181 gcgattttat gcctgccagt ttgtacataa gctgagtgtt tacaccgccc atacataaac
     3241 ccaccagcaa cgtaggccaa aaattataac cgtactttaa gccaagcatc gctatccatg
     3301 cggcagtaac tatttttacc agcttaccca accaatgaaa atgagtagcg gctttactgc
     3361 gcggtataag cacatcgtgg cggtggtata ctgcattggt aaataaatgg ccctgtgcat
     3421 tttcttcaaa aatcaagggg acaataagcg gtgtgccatc ggctttttta ttgcttgaaa
     3481 aatgacaaac attatcgtca ataaacaaac caatgggtga cgtactaaac tgcttattaa
     3541 aaattttgat ggcggcaggt ttaatttgca acaacccttt ttgtatgggt gctaaggtaa
     3601 aagtgagact tgtttctttc atggtcatac catttcgcac cgatgtacca tgatgtataa
     3661 aaggtttgcc tataacgtga aagcctctga aatcgggctg ccggaatatg cgcccttcgc
     3721 ccgaatatgt aaaggttata aatacttcat cgttaagctt tacatgtgtt ttgcttgccg
     3781 acacaacaaa ataatggtcg aatgccgggc ttgatgtatg atagtagtcg cccgataatt
     3841 gtttgtcata tgcatatcgc ttattggtgt cgctcaaaat ttcgtacgct gcctgcacgt
     3901 gcaaaaacaa ttcctgcgca ttagcattat taggattcat atcgggatgg tagcgcttgg
     3961 caagttgcct gtacgccctt tttatttcgg tcacactggc cgaattatct actcccaata
     4021 tatcgtaatg cgttttcaat ttgtttttgg tgatgattac cttttaagtt ttaaaaaata
     4081 aaattctaac aacacaaatt aattttcaat atgaatttat tcaatctcct taacgaaaat
     4141 tatttttcaa aagtatacta agagtagtta gattttcgaa agaaaatata acgtactctt
     4201 agtaatgccg gacaacttca atagcttttt ttccaaaacc taagttgttt cggtatactt
     4261 tgaaaaaaat tgggtataaa atcacccaaa taaaaccgtg atattttaat ttctcaaatc
     4321 taaaccccag ttgagtttat tgcgcaatgt gccaagaaaa ttttcgttat cgagccgaac
     4381 aagattgagc ataaaatctt ctttgcgtgc cgccaattga atactgctat cgatggtaac
     4441 tgtgcgcgaa tcgagcgatg cctgaaaata ataactcctg ccttgcacct cgagcgatat
     4501 cacacttttg tcggaaacaa taatgggtcg tacatttaaa ttgtgtggcg ctatgggcgt
     4561 gatgacaaaa ttttcgctct gtggcattat gataggccct ccacaactca tgctgtatcc
     4621 ggtagaacca gtaggtgttg ctataataag tccatcagcc caatacgaat tgaggtattc
     4681 gccattcaaa taagtatgca ctgtcatcat actggcgctg tcttttttat gaatggtgag
     4741 ttcgttgagg gcgtagttta cttttccaaa aagtgttttg ttgctttcga gtcttaatag
     4801 tgtgcgaggg tcgagggagt ataaaccttt ttgcaaacag ttgatggctt tgtgtatttc
     4861 gcttttattt atgctcgata aaaatcccaa tcttcctgtg ttgatgccta acacaggtat
     4921 gcccgaatcc tgcaccattt cgatagtatc gagtatagtg ccatcgccac caatgctaaa
     4981 aaatatatct accttattca ctatgtcttc gttggcagca aaaacaggaa gctcaatttt
     5041 ggttgtggca tggcggttta taaaattcag aaacgattcg tatatccaca catcaacaga
     5101 agcctcgtgc aaagctttta ccaattcctc gataaaagga accgaagtaa cattttgctg
     5161 acggccatat aatgcaacta tcatcttttg taattaataa atgagttgtt cattaccggt
     5221 ttctataaca agcttttttc ctttatgcac gctacagtat ccaattattt gtgcatcaat
     5281 atttaatcct tttgcaatgg ctatcatttc ggtagcggct tctttgttag tgtaaatttc
     5341 catgcggtga cccatattga aaaccttata catttcgtgc aaggctgttt gcgattgatg
     5401 cgctattact ttaaataaac ccggtacagg caataaatta ttttttgtga tacatatatt
     5461 atcggcaaag tgcatcacct ttgtttgccc gccaccactg caatgtacta tgccatgtat
     5521 gtgttgtcgc cacgatgata aaatattttt tatcaatggt gcatacgtgc gtgtgggcga
     5581 taaaactaat ttacctacag tagtttttac cgcaaataaa tcatggttgt aattgtcatc
     5641 tatcaatact ttttcatcgc gcattatttt gtagttttca tttttatcga tataaattat
     5701 atcggtaaga aaataatttc cactgtaaat aatatcgtca ggcaaagcag tatcgtaagt
     5761 ttcgggatag gatttggcca atgatttttc gaacacatcg tgccttgcac ttgtaaggcc
     5821 attgctgccc ataccaccat tgtattcgct ttcgtaaatg gctttgccgt aagatgcaaa
     5881 acctacaata acatcaccgg gctgaatatt attttctata acatcgcttc gctttagccg
     5941 gcaggtaaca gtagagtcaa caataatagt gcgcacgagg tcgcctaaat cggcagtttc
     6001 gccaccggtg gtatttatgt gaatgccata ttcgttgagt tgtgcaacta tttcttcggt
     6061 gccttctatt atggctttta tcacttcgcc cggcacaaga tttttattgc gaccaatagt
     6121 gctgctcaac aaaatattat cggtagcgcc cacacaaagc aagtcgtcaa tattcatcac
     6181 tatagcatcc tgtgctatac ctttccaaac tgatatgtcg cctgtttgtt tccaatatgc
     6241 ataggcaagt gacgattttg tgccggcacc atcggcatgc ataatattgc accattcagg
     6301 atcgtggccc aggtaatcgg gaataatttt acaaaatgca ttgggataca accctctgtc
     6361 gacatgcttt atggctgcat gcacatcttc cttatcggcc gaaactccgc gggcggtgta
     6421 ttttgaaata attttattgt ccattttagt atgggcaaaa gtaggtttat tcattggcaa
     6481 gtgcaagttg gtggtttgta attatggctt aggataaata caaaacaata tctcaaaaaa
     6541 acaattttat atcgctttga aataaacaaa ttagaaatct catcatatat aaataaaagt
     6601 attgcatgga attatgtaat acaaccaaat ttgaaatact ttatttttgc aataccaatc
     6661 tattaaatat tgtattattg actgatgaag ttttaataat aaaaaataat gctgcatagt
     6721 atttgtaacc ttgccaatcg aactaaaaag taatatgatg atgaaaaaaa tattttgtgt
     6781 ttatgtttct ttgatttcat tgatttttat tgatatcaat ttgcaggcgc aatcgtgtgt
     6841 cagttgtcct acatgtttta ccgcaggttc cgaactggta acaaacggaa attttgcctt
     6901 ttcggcacca tatgcataca ctgccacagc accacttgta aatgcagttt atactgcttt
     6961 gccaccggta aattttggaa attattctat caccaataat cctaatacat acaattcgtt
     7021 ttggtgtggt gccgaccata gtggcggtgg cactatgatg cttatgtgcg atggttacaa
     7081 tggttcgcca acatcggcat ggggacagta catcaatatt tcgaccatag cgcccaatac
     7141 aaattttaca ttttgttttt acgccatgaa tattttatgt cccggtaatt tatcaatcga
     7201 cccattgatt caaatgtatg ttaatggagc accgttattg ggcgcagctt atacgcttgt
     7261 tgaaaataat aatcagtggg tactcataac cggcacctgg aattcgggca acactcctcc
     7321 ggcaaatgtt ttgttggaaa ttgccacaac ctcctactcc ggatcgggca acgattttgc
     7381 cattgatgat atttctttca aagcctgcct tccatctttt ttaacttcgg catcgaatga
     7441 tacagctatt tgcaaaggca aaaacgtgca actattggca gcgggtgcag ccaattatgc
     7501 gtgggctcct gcaacaggtt taagcagtac ttctatagcc aatccggtgt gcaatgtgca
     7561 ggcaactaca acgtacactg tttctatttc aaatggactt ggctgtacca aaaccgaaag
     7621 tataaccgta actgttaatc aattgcctgc tgtgaatttg ggaagcgaca caagtatttg
     7681 caatggcaat tcgctaatat taaatgcggg aaattatatc ggttatcaat ggcaaaacgg
     7741 caacacaacc gccacaataa atgtaaacag cacgggcaca tatactgtta ccgttagtga
     7801 tgcaaatggc tgttcgaaca gcgatgccat tgatgttacc gttaatcctc tccccgtttt
     7861 caatgctaca cctgccatcg attcaacctg tgccggaact tcggtttcgc tttcggtttc
     7921 gaataatact tacaactaca catggagccc cggtgtggga ttgagtgcca caaccggcag
     7981 cacagtaatt gcaaatccac cggcatcaac aacgtatact gttaccggta caaacggctt
     8041 tggatgtacg gcatcgtcaa ccgtttcgct tactataaat gcactgccca atgcaggtgc
     8101 aggtgcaaac aaaaccattt gtaaaaaaga cagcattgct ctcaatggaa gcggaggcgt
     8161 gcaatatagc tggttacctg cgggcagcct cagcaatgca aacatagcca accctaaagc
     8221 cagtccacag gttacaacta cttataccgt taccgtaacc aatattaatg gttgcactgc
     8281 cacagcaaca atgaaactga aagtaaatgc cctgcctgtg gttaacttag ggagcgatac
     8341 cactttatgc aaaaatattc ccatagtgct taatggtaac aacaatacag cttatacttt
     8401 tttgtggcaa aacgcttcta ctaatattaa ctttactgct aacgatagcg ggacctatta
     8461 tgtacaagta actgatacca acaattgtaa aaatgccgat acggtggtca taaattatta
     8521 tccctcagca ttaatcaccc tcacatcgag cactaataaa ctgtgtattg gcgatagtgc
     8581 atttgtaaat gccggtggtg gcgttagtta caattggact ccggcttatg ctataaataa
     8641 tacctcaagt gccaatgtta ttttgtatcc cgattcatcc actacgtata tactcaccgt
     8701 tgccgacagc aatgcctgcc aatatgtgga tacgctggca ataacagttt tcaatttgcc
     8761 ggtggtaaat gccggtgccg acacagcatt ttgttttggc aactctgtaa acttacttgc
     8821 catgggcggt gcaaattaca gttggttgcc tgctgcaggg ctcagcgata cggcaattgc
     8881 caatcctgtg gcaactccgg ccttcactac caactacatt gtgcatgtga ttgataccaa
     8941 cggttgccac aataccgatt ctgttttgct tacagttcat gcactgccaa ttgtaaacct
     9001 tggtaacgat actacgttat gcaccacaca acaaattaca cttgcaacgg caggaacttt
     9061 caatagttac ctgtggcaaa atggcgatac caatgccaca attactgtaa atacttcagc
     9121 agcgtatata ctcagcgttg ttgacagcaa tgcatgcgca aatactgata cggttgatgt
     9181 tacttttgtg gtttgcccgc ctcctgttat tggattcagt tgcaccgaca cccttttttg
     9241 cgaaaaacaa tgcatcgatt ttaatgactt atcgactaac aatcctacaa gctggttgtg
     9301 gacttttacc ggtgcttcgc caatttcatc tacactgcaa aaccctgcaa acatttgtta
     9361 caataactac ggtgctttcg atgtgcagtt gatagcctgt aatgttggcg gttgcgatac
     9421 gcttacctta acacagtttg tgcaggaatt tcaaacgcct gctgctccgg taatttttca
     9481 gacattcgat agtttaattt gccctactac caatgttacc tatgcatggt acaatacaaa
     9541 taatcctaat aatgttttat ctactaacta ttactatata cctaccgtac cgggaaatta
     9601 ttatgtgctt attgccgata gcaatggctg tgctgtagcc tccaatatca ttggcagtaa
     9661 tgtgggaatg tatgataata ataacataca gggctacacc attataaacg atggcgctaa
     9721 tttaattata gaaaccaaca ctaaacaatt aatacaagca aggattttta atttggcagg
     9781 gcaagttgtg aagcaacaat tttttaacaa tagcactttg aaatatattg ttcaactgcc
     9841 gcctgccagt ggaatatatt tattagaatt ggttgatagt gacggtgggg ttgtgcatta
     9901 cagggggttg tcgaaataaa gcataataaa catgttttaa ttctaaggac tgcacaaaac
     9961 ttcaagaagg cttatcatag agataaacaa aatgcgattt gatgactaaa gttcaatatt
    10021 gttccgattt gcatttagag tttcaggaaa ataaaaatta cctgaaactc tttccaataa
    10081 ggccggaggg agaaatactt ttgcttgcag gcgatatttg tttactaaaa acgatgaagg
    10141 agcacgatga tttttttaat ttttgttccg acaattttga aaccacatat tggatagcag
    10201 gaaaccacga atattatcat tctgatttat ctgatagaat agtaaaaagt gatgaattcc
    10261 aatcaatgca tgaaaagata agagaaaatg tttttctcat aaacaacaaa accattcttc
    10321 acaaaaatgt ggaatttttg ttctccactt tatggagtta tatccctcag ttatatgaaa
    10381 aaattgtgca acgctcggta agtgattttc accttataaa acaaaaaggt aaacttataa
    10441 cagcgcaaga ttttaatgaa atgcacaaat gcggtttcga ttttttacaa tctgaattgg
    10501 ctaaaccaaa agacaaacag cgaattataa ttacacatca tgtaccaaca ttgatgcatt
    10561 atcccgtgca gtataaaaac agcaacatca attttgcttt tgcaacggag ttatttgaat
    10621 ttattgaaac ctcaaatgct gactattgga tatatggaca ccatcattgc aacacacctc
    10681 catttcaaat tggaacaaca accatgctta ctaaccaact gggttatgtg aggcaaaacg
    10741 aacattcaca gtttgattgt gatgcaacaa ttgaaatttg aaaagaaaaa aaatattttt
    10801 attaaacttc aaaaagaaaa cccctacaca aaaaatgtat aggggttttt tattttatgc
    10861 aataattgtt tttactacca actcacaagc gacaacccta ttgtaaaagg atgtagtgcg
    10921 gggccactat tttgtttgaa aagttcgttg atgccgtatt gcgcaaaaat attgtagtta
    10981 cggaaaccta tgcaaacgcg gccatcgagg cggaaaggat tgatgttgaa atcatcgcgg
    11041 gttttattta caccatcgga tgttttgtac ttggtatgtg cccccaattt atacgcacct
    11101 actacaccaa agccaaggtg tatcgctttt ttacgtaatg ggttggtaca tatttcgaac
    11161 attgccggta tggtaatata acttacggta agtttatttt tcgacaggtc tttgcttatt
    11221 tctaatgcct gcactgtatc ggttttagca atcaatacac ggttattgtt atcgaacctg
    11281 tagttgttcc acataaaacc ggcaccggta ttgaaccata agtatcgctt aataagtttg
    11341 gcttttattt caatcagatt gagattaaca gctatggact tgcctgagtt tagctgaaga
    11401 aaatcgtagt ttgccggacc gtcatttgca cggtcgagcg catctatacc tggcacataa
    11461 ccattgaatc ctaattcgag gccgccccaa tgcggttcaa tatgatcatc tttgtgtaag
    11521 ccctttttta catctacatt tttcgaacca tcgtcatcat cattttcgcc cgacacattg
    11581 atgttgatat ttccaatact tattcgggta gtgtcgtttg cgcttttatt ggctacatca
    11641 tctacacgtg caatgcccga attgtttgcc gacattttta aaggttttgt tttgtaataa
    11701 atagtagcac tgccgctaac atttgtatta agttcatcgg taacatctac tgtagttttg
    11761 caacttccgc taatgttggc atcaaacttt ttaactttca attcggcagc atttaatttg
    11821 ctgatgcccg aagtattaaa ttccatttcg ttagcagtac ccgatatttc gcctttggat
    11881 acaccgcttg tgtttaccga tattttattt gcagttactt taaggttaag agtaagtttg
    11941 cccgatacat taataagcaa atcatttatt acaattggca catcgctgct tacctgccca
    12001 ttgccggaca ctttgagttc ggtaagatct ttaataccta cttcaaccac gttggccaaa
    12061 gggctcgaca cagtgagcac attgttgttt acggtaaaaa tgctcgatat atcgttggcc
    12121 gtagcagtaa tggtatacga ttgcgaaggc accagtttta tgtttgcagt gctgctgata
    12181 gaaattttat cgaacggctc tatttgtata gtttgctgcc ccattgccat tatctgcaac
    12241 attattaagg cggccaaaaa gctaatcttc ttcataagta tatttttgta ttatttaatt
    12301 gtaagtatga caatgataca aagtaaaaag ttacaaggca tagttttaaa aattttgata
    12361 aaacatatta caatattttt cgtagtattt cattcacttc accggctttg ttcattagca
    12421 taaaatggcc accattttta attacaaaat cggcttttat attttggatg gggaatattc
    12481 tgtcgctact tccatgtatg tgtatcacat tgcccgggta agtttcattt tgccaatgaa
    12541 taattttatc gatactccat ttcaaatatg caatatcgat atttgttaca taggtattaa
    12601 ttagattttg ctcatcgctg cttgttacac caaacataaa gctggctgtg gttttcattt
    12661 tttgcatcca ggtaagggga aaatatttgt gtgccggaat tttctgcagt gctttcatgt
    12721 aagtgggttt ttcgtattgc gtttttatgg tcgatagcaa aattattttt tgaggcttat
    12781 aaactttcgc aatttcaata gccaccatgc caccaaatga taagcccatt aacataaatg
    12841 gtatccctgt atcgattgtt gctgccatgc gcatagcata ttgttgcagc gattcgcttg
    12901 ttataaccgg cagccatggc aagtgtacaa ccgtgtgctg tgcaaagtag gtatgacaaa
    12961 agactttttc atcggcaccc aaaccactta taacgtatat atgcatagtt acattctttt
    13021 ttagaagtgt acacttaggt gctgtttata aacttgttaa taataaaagt acacttaccc
    13081 gaatagcaaa taaggcaaga ttacttttgt tacgaaattt ttttttgtaa gtaattaaat
    13141 atttctaaag ctttttaaaa ttcagccccc gtttgtaagg tttcgggggc ttttttcatt
    13201 ttatacactt gctatacagt aatttcgttt taacaattta ttttgtgttc acatggtttg
    13261 tggggataca aaccatggca ggcgaggact cacgaatggt ttgtggggac acaaaccatg
    13321 gcaccaagga cacaaaccat ggctggcgca gcaatgacca atgacagaat taaatgacca
    13381 atgacaagaa ttttaaacag tcataatttc tttttccttg gtttccaggt gcttgtccac
    13441 ttttattatg taggaatcgg taaggtgttg cacttttaat gtggcttctt tttccacgtc
    13501 ttcgggtagt ccggctttaa gggctttttt caatccttcg ttggcatcgc gcctggcatt
    13561 gcgtattgtt actttacaat gttcgccttc ggctttggtt ttctttacca gatcttttct
    13621 acgttcttcg gtaagaggtg gcacagcaat gcgaataaca ataccatcat tttgtgggtt
    13681 gagtccaaga ttagcagctt gtatagcttt ttcaataggc accagcatcg atttttccca
    13741 gggttggaca accagtgtgc gcgcatcggt agtatttaca gtgcccactt gattgagtgg
    13801 agtgtttgca ccatagtaat caacatacaa gccatcgagc atttgtggcg atgccttacc
    13861 ggcacgaatt tttgataatt ctgtttcgag atgatcgagt gcattgttca tttcgccaac
    13921 ggcatgatca aatatttttt ttacttcttc catagtttat ttttagtttg agaggaggca
    13981 acaggtgttg caattatatc gcgaatttgc aatttgtatt tttagtacac aagatttttt
    14041 tattgctcat ctttgtctct ttccttattt tattgaatat agtttacttg agtttttcaa
    14101 tgctccaaaa aataaatcgt tcaatttgga tttattgtta cttccatttt tcgggttaac
    14161 aaggattgta aaacctaaaa tcaagtttga gataattttt tttgaaatta tatagtaacc
    14221 agtgttccaa ttttttcgcc ttgtaccaga ttaagtaagt ttcctttttt attcatatcg
    14281 aaaactataa tggggagttt attttcttcg cataaagtaa atgctgtcat gtccattact
    14341 ttgaggtcgc ggtcgtatac ttctttaaat gttacactat caaattttgt tgccgtggca
    14401 tctttttcgg ggtcggcagt gtatatacca tcaactcggg ttcctttgag tatcacatcg
    14461 gcttcaattt caatagcacg caacgatgca gctgtatcgg ttgtaaaata aggattgccc
    14521 gtaccggcac caaaaattac cacacggcct ttttctaaat gacgcacagc cctgcgcctg
    14581 ataaatggct cgcaaatagc ctccatctta atggcgcttt gcaaacgggt attgacttct
    14641 atttgttcga gtgccgactg caaagccatg ctattgataa ctgtagcaag catgcccata
    14701 tagtcgccct gcacgcgatc cataccgctt tcgctggctt gtaatcctct aaaaatattg
    14761 cctccaccaa ttacaatggc aatttcaact cctgcagcct gtacatcttt tatatcctgt
    14821 gcatattgcg atatacgttc ggtatcgatt ccaaattgtt tgttgcccat gagggcttcg
    14881 ccacttagct ttagtagtat gcgtttgtat ttcatattat atattggatt ttttttgcga
    14941 tggcgaaagt agaaattatt ttttcattgg tttatgagaa tgtgtgatgg ttcataaaaa
    15001 ataaaatctg cggtttaaaa actcttaatt tattgaacca cactaaaatg aagcccacta
    15061 cgttatgtaa aatctgtatc ttgcgggcat gaaaaaaagt gtattatttt attttggatt
    15121 aatagcaacg ctgctttggg gagcatgtaa caaagcggac gaaataggca acggtatttt
    15181 accggccgat gatattcccg gattattgtt tagcgacaca gccagtattg taagtttctg
    15241 cattaccgat gactctataa aatcgggtgg ggttaccaat aaaacctttc ttacatcggt
    15301 aaatccggtg ggcgcccaaa acgatgctac ttttggtgca accgttgccg ggctttatgc
    15361 acaagttgat cttagtaaaa catcgttcga ttttggcacc aactttattg ccgatagcct
    15421 ggtacttaca atggcttata aagaagtttt tggcgatacc aattcgcctg taacttttca
    15481 tgtatatcgc ctcaacgata aaataagcag cgattctatt tattttacca gtaaggatta
    15541 cttcacttac aatgcacaag aaataggaag tgccacagta gtgttaaggc caaaagacag
    15601 cataacagaa tatgacgtta aacgtgcacc acacataagg ataaatctgg accctgcttt
    15661 gaccgtggag tttgcatcca aaacacaaag caacgagctt aagataaacg atgatttttt
    15721 aaatttcttt aaaggaattt atatcacagc cgatgtgcca agtgctgcag gcagtggttt
    15781 tatttttacc ctcgattaca aatcagtagc atcgcgcatg gcattatttt atcacaacga
    15841 tgctggcgac tccgcagtat ttgcttttcc tttcaatgac tacaacaggg taaacagatt
    15901 taaacatgat tatagtggaa ccgctatagc aacacaactt gtcgactcaa caacaggagg
    15961 cgattattct tatttacaat cgcttggtgg tttgcaaaca aaaataaaat tgcctaattt
    16021 aaaagattat aatcttataa ataaaagagc aatcaataaa gcacaactaa taattcaggc
    16081 cgacaatagc gaggtgacaa aatatgctgc acataaaacc atttcggtat ttactgccaa
    16141 tggaagcaat cagataacag acaagtttag aatattaccc atggcttatg acagtgtaaa
    16201 gtcgcaatac atagccgata ttacagtgta tgcacagcaa gcgctcacgg gcaagtatga
    16261 cgaccttggt cttattttaa aagctaatgg agcattggta aatgcatacc gcacacgttt
    16321 gtttggaaat agtccaatag ataagcgcat gaaattgaaa ttgtatttta ctgagtatta
    16381 attttttctg atatctgtaa gtgtatacta ttgtaaattt aacagcgtta gaaacttaca
    16441 tttaaaatac ccacatcaac atggtcaaga cattactcac tggtacgcta ttgctaataa
    16501 ccctgacgtg ctttggtcaa caaaaaattg gacaatggcg tgttcatctt tcatataacc
    16561 aaacaaaagc gataacggtt tcgcccacaa aaatttatgt tggggctaac gatgctgtat
    16621 tttattatga taaggaaaac aatgaaatta aaacattgtc gaaagtggaa ggtctttcat
    16681 cgattggaat aaatacactg aagtataatg attacaacaa atgtttgctt atagcataca
    16741 gcgatgccaa tatcgatttg ctgaaaggta aaaccataat aaacatatcc gacataaaac
    16801 gaaaaaacat aattggcggc aaaaacataa ataatattca cttcaaaaac gagtttgctt
    16861 atgtatcgtg cagctttgga atagtagtaa taaatactga tagaaacgaa gtaaaagaaa
    16921 cgtatacctt gggcagtaca aattcaaata taaaaatatg gagttgcact accgatgata
    16981 attatattta tgctgcctcc gattcgggcg tttatcgtgc aacgcttacc gatcctttta
    17041 ttgtaaatcc cactgcatgg caaaaagtat ttgcaataaa gcccggcagt ggtggggtaa
    17101 aatatattac caatttcaac aataaaatat ttgctaatca ggtacaaaca accaccctcg
    17161 attcgctata cataatggat attgccacac aacaatggag tggatttgca ataggtgcaa
    17221 acgaaacaca aacaaaattg agcatgcaaa gcagtggcga tgtgcttacg cttacaagtt
    17281 tatattttgt acacagtatt gacaaaaatt tcaatgtcac cacttacaac aacacaaata
    17341 ttgcattcac cactgccaac gatgccgcag aaaataatgg taacatttgg attgccgatg
    17401 ccaataaagg gctgataaaa tatactcctc aacaaggcag cgaaataatt gtgccttcgg
    17461 gtccgcggtc gaataaagtt gcgctcattg atggttcgga aaataatatt tggattggac
    17521 atggcccgcg tttcaacaac tgggcacctg ctggtgtggt tgatggcttt gcaaaatttg
    17581 ataacacaca atggacaaca tttcatggca atgcacccgg caatagtttt tcgctcgata
    17641 ctgtagaaga tttgatttgc attgctgccg acccgtccaa taaaaatcat gtgtactttg
    17701 gttcatgggg tcgtggttta gtagagtatg ataatggcac catcaaattg tataacgatg
    17761 ccaatagccc cattaaagtg caacagttta ttgccaacta caggcactta cgaattggag
    17821 gtttagcact cgatcaaaat aataatttgt ggataacaaa cacattatcg gattcgggat
    17881 tgattgtaaa aaagaatgat ggaacttgga aaaaatttta tgtcccgtta ttatcaggag
    17941 gagattatgt aacagatatt attgcagatg actataatca actgtggata aatgtgccca
    18001 taagcggagc aaacaatacc ggcattttgg tttataatca taatggaact atcgataatg
    18061 tgagcgatga cagagtgcgt ttgcttagcg atgaagaagg caaaggacag ttgccaagta
    18121 aagctgtgcg ttgcatggca cgcgatcagg atgggcttat atgggtaggt accgaaaaag
    18181 gagttggtgt tttttattct ccttcttcgg tcttcagtgg tggcaattac gatgcacaac
    18241 aaatattaat agtgcaggaa acttacaact tgtacttgct cgataaagaa gtagtgcagg
    18301 ccataaatat agatggtgct aaccgcaaat ggtttggtac acaaagtggc ggagcatttc
    18361 ttacatcgcc cgatggagta aatcaggttt tgaattttaa tgaaacgaac agccctttgt
    18421 tgtcgaataa tatctacagc ataaaagtaa acgatgcaac gggcgaagtg tttttctcta
    18481 ccgatagagg attaatatct tatcagggcg atgccactca aggttcggca gcgtgcgaaa
    18541 acgtgctcgt atatccaaac cctgttcgcg aaaactatga aggaccaatc gcaattcgtg
    18601 gattagtaaa taatggcaat gtgaaaataa ctgatgtaag tggtaatctg gttttcgaaa
    18661 cacagtcgct tggtgggcaa gcaatttggt atggaaaaaa tttgaaaggc gaaaaagttt
    18721 ataccggagt ttatcttttg ttttgcagcg atgccgatgg cacaaacaca tgtataacca
    18781 aattgctttt tgtgcgttaa aaataattcc gaataattcc tatcttttga tttgcaaatt
    18841 tcgacacaag gcatagtact gcaaacagta aattatagcg acactgctgt tattgcaaaa
    18901 atatttaccc tccatcatgg gttagtttcg tttatggtaa aaggagtgcg gaaaaaaaat
    18961 gcacgctttg cagcaaattt attttctccg tgttcgatat tatccatgaa ttatttattc
    19021 aaagtgaata aaaatttaca attcgtaaat gaaatatcat gcgatgtacc tctcacaaat
    19081 ttacaatcga atccactgaa aagttgtatt gcttttttta tggcagaggt ttgcgtcaag
    19141 tctattaaag aacaagagca ggatgaattg ttatttgatt gtataaggca ttttattgag
    19201 ttactcaacg acacagaaga tagcataaaa caatttcatc attatttttt gttgcagctt
    19261 gccaataaat tagggttcgg gccacaaggc atttacaatg atatgcacac ggttttcgat
    19321 ttgcgtgaag gcatctttca atcttcttat ccggtatatc ctgaatttgt aattaaccgc
    19381 acggcagaat taatttcgct ttttacaaat agtacttttg aaaacagcat aagtataact
    19441 gcatcaaaag ctgaacgcaa ttcgttactg gcgaccctgc ttttttattt cgatttacat
    19501 gtattgcatg gaagcaaaat aaaatcgacc gatgtgttga gcgaaatgat ggcttaaatt
    19561 attagttatt ttctacatcg gtaacccctc ccgaaataat aaatttcatc atctcggttc
    19621 ccgatgcatt tatgggcgaa atatttgcta ccggcactat taccaattgg ccattaaagc
    19681 cataagagaa aggcaagtaa acagctacct ttccctgagg tattcccaaa aagcctaaat
    19741 catcttgtgt aacaaaccct atgcgctgca cttgagcttc tttatcaacc attaccaaca
    19801 caggatgatt gaatctcttt ttttcgccaa caaatgctgc tatcaaatct tttattgaag
    19861 tgtaaactat ttttaacaaa ggagtacttt caaaaaaatt gtcgatgtaa taaagcaccg
    19921 gattgcgaac aaaacgtgta gtgatataac caaggatggt aacgccaagc attatggaca
    19981 aaattcccaa acctggcagc tcgaatgtgt aaccatcgct tattttaata ggaatgtgaa
    20041 aaggcagcaa tccatcaatc cagcgtatgg cattaaaaaa aataattatg gttaaagcaa
    20101 ccggcactgt tatcaatgca ccttgcataa aaaacgtaaa taaacttctg aaaactttct
    20161 tcattagagt gattaatctg ttgttgcgca aaagtaacta tacaaaatca tgttgagcta
    20221 ttaattacat tttgctacct atttatttta agccttcata ttcaagcttc acttttttgg
    20281 ggaggtcatt tactatcaaa tcatacgaat cgtctatcca tttctttatt aaaacatccg
    20341 ttaagccctg cgccatatca atagtattcc aatgcttttt attcatgtga taaccgggct
    20401 gcacggcatc atacttttcg cgcaaatcaa tggcggtttc gggatcacat tttacattta
    20461 ttttttcggg ctcgcttaag ctcgtgagta aaaacatttt tcccatcacc ttaaacacta
    20521 aagtatcgct atcgaaagga aatgattctg ttaccccttt cttattcata caataatttc
    20581 taatcaactc tatattcatt cttctatagt gtgtttaata aatttaattt cttttacaac
    20641 ataaatccca aagcagcctt tgtctacttt ataaacaacc tctttccatt gtttccaggc
    20701 atcgtcatac ttcattctac gcacaaaata ataattatcg aagagtccct ttttatactc
    20761 tacaatagcc ctccacttat cagggtcggg aggcgtattg attgtcactt tcataacctc
    20821 caactgtata gattttggct cattaagtgt tatgttgcta ttgagaaata aagccaacat
    20881 cattaaaggc atacccaaaa cggttataca aacaaaagca agattaaatt tatttgaaat
    20941 agattgatag cccatccttg cattaatgaa gtaataaaaa cttagcccta aagccacaac
    21001 tatcaagata gtatttgtaa tagaaataag ttgatggtcg agcaaataca taaaaggcac
    21061 ccaaccgcaa acagcaaaaa atccgcttaa caaaaaagga aaaacaaaat ttgaaggttc
    21121 aaaatttcgt gcttccagcg aaatgttttt cggttttgtt tgaacttcct gttgcctgcc
    21181 aattgcattg tggtgataat aattccctct actatttatt ggattttggt ttaataaatt
    21241 gcaaagagca tcatataagt cggggtaatt ctgcattaat ttttgtggta cttcgaaaaa
    21301 gtattctacg catacggcaa aaaactcatg aggatttgtt cctgcatact cgcgtaaaaa
    21361 tgattcggct cctgaatttt gcatttttaa aaaaatttca ttcccaactt tttcccattc
    21421 ctgtgtaaga ttttgcaaat gcatgtcaaa ctgcttagta gtgtcgatac ataatttgta
    21481 cacatgagca aactcatgta gtcctaaatt gtaattgtcg tcagggtgta agtatccttc
    21541 ataaaaatta tcccatgata tcattactaa tccgctttta aaagttcctc ctttcatgta
    21601 aagttgcgat cgtggtgcat aaaacactcc gggagttaca attattcgcg aaaaattatg
    21661 catatcaaat tcttcgagcc caaatgtgat ttgtgttaac gggcaacata aaagatattt
    21721 aacttcatca gtcatttcga aaccgtctgt accagcaaat tcaaaatttg aagcaaagta
    21781 ctgtaccctt tgcacaaatt ttatccgatt attaacatcg agcttcttat aataaattat
    21841 tataggttct attacctgat gaatgtaatc atgttcatgc ataaacttta caggttagtt
    21901 tatttgatta gcaaacaagg ttataagttt aaataaatcc acatattcgg tgagcggtaa
    21961 tgttttggca atatcgtaaa catcgtggta agcttttatt cccccaagtg tataaataaa
    22021 aaaagaaggc accccttttt cggtaaacca ataatgatca ctgttggcag ccttgcctcg
    22081 tttatctacc ttggtcagta aattattatc attattaata gtaaccattg ctgtatacgc
    22141 ttcactaaac tcggtagcat ttaccaccat aattccttcg tcacccgtgc ccattaaatc
    22201 gaaattcaac aaaaacttaa ttctatataa ttcaaataaa gggttttcgg tgtaataccg
    22261 tgaacctatt aatccggcct cttcgcctgc aaaagctata aacactacgg tatattttgg
    22321 tttgtgcttt gaatagtgct tggctaagtt cagtagaaag ctaatgccac ttgcgttatc
    22381 atttgctccc gggaaaatgg tcttctttcc catcatgcct aaatgatcat aatgcccggt
    22441 gtacaccacg aaactatcct ttacttcacc ttcaataaac ccaataacat tttggctttt
    22501 gtgatctggt aatatcaagg catcaatatt tacttttaat gttttagaat ttttgttgaa
    22561 actttgcttc aataccgaaa ctgaaaattt atttttttga ctggttgata cactccacgt
    22621 cagctttttt tcttctaaat agatgaaacc aatattgctg cgattttcgt ttatatatgc
    22681 tcgcaccact ttctttaaac tgtcgctgaa taatgaaagg tcaattagca caatcttatt
    22741 atcgagtttt gattttttat accaatcatc aactgtcaac aactccacta tttcaaaagt
    22801 gcctgttata gcggggcagg caggatcaac aataaaatcg atgccgggaa ttagtgatac
    22861 cccatcggct acaatttcca ttttccccgg aaaagtgttt acgggaaagc taaagtcttg
    22921 attatagctt ctgccaaatg gctttaattc aaattttaaa aattctttgg aaatgaatgt
    22981 ggctgcaaca tgttcaccat ttttgacata acccctaccg gcatattttt ttgaggttaa
    23041 cgtctctatt acacttttcg cgtaatttat atcctgagca acaatgtttg ttgtgtaaca
    23101 caacactgca aaataaaatg tataaatact tttcatgcct tcgttttaga aagggtgaaa
    23161 ataagcaaaa aaaaaccgca agtaataatt gacttgcggt ttcatttaat ttgctattag
    23221 attattgctt aacaattttt actgttcgtg tcaatccatc aatcctcaat tgaacagtat
    23281 aaattccctg tgcaaaacta gatgtattat aacggaatgt attcgcacct tctgtggcta
    23341 aactttcttg cctatatact actctgccag tcatatcgaa aatcacaaca tcgaattttg
    23401 tgtcttttga tgatataaaa cttattgcta actcatccaa aacaggattt ggatatgcat
    23461 taaaactcaa ctcatcgtca gtactaaacc atgttgagct tttactacaa ttattcacag
    23521 gacaagtgag aacattgttc gatttgttac caatatacca agatgaattt ataaaagcag
    23581 cgtaagtccg taatattgaa ttggaatatg cagagccaca ccctgttaaa gcattaacac
    23641 cttcattgaa tatctgctga actgttaaac ctgttaatgg tcctgagtct accactaaat
    23701 ctttaaaatt aacagtacta ggtgcaaaat taggattagc taaatcaaac tgaatgttga
    23761 gtgccaaagt tacaagttga gcagcaagtg aattatttaa agctaataac gttgggtcaa
    23821 catatgactg agttaacgct gcaacattaa ctgttccttg catagtaata cgtgcagcag
    23881 cggctgaagt aagtaataaa gacttagcag tgccgcaagt tccctgcaat tttaatcctg
    23941 ctgggaatac ggtgttaaag ttagcatcta agtatttata caatgtgtcg ggcttccaat
    24001 tcgaattcca atacgtttgt gcatgacctg tatagaaggt agtacatacg tatgcaggtt
    24061 gcaaaataac tattgatccg gttttggtac aacctttact atctgtcacg gtcactgtat
    24121 acgttcctgc aacaagtcca ataatagttg aatttgtgcc accattgctc caaacataag
    24181 tgtaacctgg cgttccacca ctcaccgaag ctgttgcggt tccatcatta ttgttacatg
    24241 acgaagcatc ttttcctgac atcacaacaa caatttgtgt aggttgtgta acaacttttg
    24301 tacatgatga tgtacaacca ttcacatctg ttattgttac tgtataagtt cccactgtaa
    24361 ctccagaatt aattgcagtt gtttttccat tgctccataa ataagtgtaa ggagaagtac
    24421 ctccaccggc taaagcagtt aaagacccat tatttgcccc aaagcacgaa acattattat
    24481 ttgtagtaat gctgcaagta actgtagaag atgccgaacc aactatagct tgtgcagttc
    24541 ttgtacatcc tttagaatct gttacagtta ctacaaaggt accactacaa agcccagatg
    24601 ctgttgcagt agttttaaca ggaattgaat tccaggaata agtatatgga gcggtaccgc
    24661 ctgctggagt tgcagttgcc gtaccattac aaataccaca agaagctggt gttgtagaag
    24721 tgcttgttgt taatgctgct ggctgtgtta tggttactga acatgtggtg gtacatccgt
    24781 ttgctgaagt aactgtagca gtatacgttc ctgctgctaa tcccgttctg gtttgggtgg
    24841 tcactccatt attccataag taggtgtaac ccgaacctcc cgtagcattt actgttccgc
    24901 taccgttagt gcctccgtta cacgatacat tcgttccggc tattgtacat gctatactgc
    24961 ctgttgatcc taccaaagta gtgcatgttg aagtacaccc tttactgtct gtagtggtta
    25021 ctgtgtatgt gccgccacat aatccgctta tgctggctgt tgttgctgca ttactccata
    25081 agtatgtata tgttggtgtt ccaccagttg ctgttacagt tgctgttcca ttacaaactc
    25141 cacaggcagc tgctgttgaa gaaacactgc atgttaatgc tgccggctgc gttatggtta
    25201 ccgaacatgt tgtggtacat ccattggccg aggtaactgt agcagtatac gttcctgcgg
    25261 ccaatcctgt tctggtttga gtggtcactc cattattcca tagataggtg tatcccgaac
    25321 ctcctgttgc atttactgtt ccgcttccat tactgccacc gttgcacgat acatttgtac
    25381 ccgttatggt acatgctata cttcctgttg atcctaccaa agtagtgcat gttgaagtac
    25441 atcctttact gtctgtggtg gttactgtgt atgtgccgcc acataatccg cttatgctgg
    25501 atgtggttgc tgcattactc cataagtatg tatatgttgg tgttccaccg gctgctgtta
    25561 cagttgctgt accgttacaa actccgcaag tagctgttgt tgatgcaaca ctgcatgtta
    25621 aagctgccgg ctgtgttatg gttaccgaac atgtggtggt acatccgtta gctgaagtaa
    25681 ctgtagcagt atacgttcct gcggccaatc ctgttctggt ttgggtagtc actccattat
    25741 tccataaata ggtgtaaccc gaacctcccg tggcatttac tgttccgcta ccgttagtgc
    25801 ctccgttaca cgatacattc gttccggcta ttgtacatgc tatactgcct gttgatccta
    25861 ccaaagtagt gcatgttgaa gtacaccctt tactgtctgt agtggttact gtgtatgtgc
    25921 cgccacataa tccgcttatg ctggctgttg ttgctgcatt actccataag tatgtatatg
    25981 ttggtgttcc accagttgct gttacagttg ctgttccgtt acaaactccg caggctgctg
    26041 ttgttgatgc aacactgcat gttaatgctg ttggctgagt gatagttacc gaacatgtgg
    26101 tggtacatcc attcgctgaa gtaactgtag cagtatacgt tcctgcggcc aatcctgttc
    26161 tggtttgggt agtcactcca ttattccata agtaggtgta acccgaacct cccgtagcat
    26221 ttactgttcc gctaccgtta gtgcctccgt tacacgatac attcgttccg gnnnnnnnnn
    26281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcatttact gttccgctac cgttagtgcc
    26401 tccgttacac gatacattcg ttccggctat tgtacatgct atactgcctg ttgatcctac
    26461 caaagtagtg catgttgaag tacacccttt actgtctgta gtggttactg tgtatgtgcc
    26521 gccacataat ccgcttatgc tggctgttgt tgctgcatta ctccataagt atgtatatgt
    26581 tggtgttcca ccagttgctg ttacagttgc tgttccgtta caaactccgc aggctgctgt
    26641 tgttgatgca acactgcatg ttaatgctgt tggctgagtg atagttaccg aacatgtggt
    26701 ggtacatcca ttcgctgaag taactgtagc agtatacgtt cctgcggcca atcctgttct
    26761 ggtttgggta gtcactccat tattccataa ataggtgtaa cccgaacctc ccgtggcatt
    26821 tactgttccg ctaccgttag tgcctccgtt acatgaaaca ttggttccag ttatagtaca
    26881 tgatattgct ccaacagctg taacaatagt tgtgcaggtt gcggtacaac ctttaccgtc
    26941 agttgttgtc actgtatatg tgccactgca taatgctgtt aacgaagtta aagttgcacc
    27001 attactccat aaataagaat atggtaaggt acctccattt gaagtaacat ttgcagttgc
    27061 attgcatacc ccacaagaag cattagtaga agttgcgttg cacgataatg ctgttggctg
    27121 agtgattgtt aacgaacatg ttgtagtaca tccgcctgat gatgtaactg ttactgtata
    27181 tgttcctgca attaatccgg tatttgtttg atttgttgaa ccattagtcc atagataagt
    27241 atatccgctt cctccaatag cagtaactgt agcgatacca ttacttccac cattgcatga
    27301 aacgttagta ccatttatag aacacgaaag gccggtgcct cctgaaatag ttgcttcaca
    27361 agtagtagta caaccccggc tatcagtggt tgttgcagta taaactccgg cacagagccc
    27421 atttctcgtt ttggttgttt gaccattgtt ccataaataa gtatatggag caaccccccc
    27481 aacaggggtt atggttgcag atcccgtgca cccacaagca acgttagttg caaaaatact
    27541 acatgtaagt aagttagggt tgcttagtac aaccttgcat gaagtagtac aaattcctgc
    27601 agtaacagta actgtatacg ttccggaaga aagtccggta atagtagatg tagtagcggc
    27661 attgctccac aggtaagtgt aatttgcacc tcctgtaggt attactgttg cagttccgtt
    27721 actgccatta ttacaggtaa tatttgtttt agttgttgtg caactcatag taccagcgcc
    27781 actcacaact gctacacagt ttgaagtaca tcctttgcta tcagtaacgg taacagaata
    27841 acttccagca cataaccctg aaatagtggt tgctgtacta ccattatacc ataagtaggt
    27901 gtagggagct gtgcccccta atgaggacac agtggctgtg ccaatacatt gcccacaaga
    27961 tgcattcgtt gtaagagcag agcaagttaa agctgcgggt tcagttatat ttattgtaca
    28021 aacactggta catccattcg cttctgttac agtaacatcg tagtttccta aagttaaacc
    28081 agttgctgtt gctgttgttt gaccgttatt ccaaaggtaa gtatacgaag ttcctccaaa
    28141 ggctgttacc gtagcggttc catcattgcc gccattgcaa ctaatgtcct tcttagtcat
    28201 gctacaagaa atcggagttg ttgctcccac ggatacagta catgtcgttg tacatccctt
    28261 tttgtcagta acactcactg tataaactcc tgagcataat gccaaaatcg tttttgttgt
    28321 tgagccggta ctccataaat aagtatatgg aatagtacct ccggtggcag taatggttgc
    28381 tgttccattg cataccccgc atttggcggg agtcgaagtg gttgcacatg ataaaatagc
    28441 aggttgagta atcgtaacat tacaactggt agtacagcca ttatttgctg aaaccgttac
    28501 ggtatatgaa cctgctccca aacctgtaat tgtttttgtt gtgctaccat tgctccagag
    28561 ataagtataa ccagcaccac ccgttgggtt tacggttgct gagccattag ttcctccgtt
    28621 acaacttaca ttagttcttg aaattatgca tgataatgga tttgatgttc ctacactgac
    28681 attgcaaatt gaagtacatc cagtagcatc tgtaacagtt atagtataca aaccaccaca
    28741 caatcctgat aaacttgtag ttgtgctacc attattccat aagtaagtaa agggaggatt
    28801 gcccgatata gctgtaacag aagcagatcc attacaaaca ccacaagaag ctaaagttga
    28861 ggtggtagtg caagttatcg cggctggttc agttattatg actacgcatg aggaggtgca
    28921 tcctgggcca gcagaaacag ttgcagtata tgccccggct acaaggccag ttgcagtttg
    28981 gttagtttga ccattgctcc ataaataact tgttgcggtt gctccgctcg cgttaacagt
    29041 agcagttccg ttatttaatc cattgcagct agcatttgtc cctgatattg tacatgaaat
    29101 atttcctatg ctattaatta ccacactgca agcagacgaa cacccattgg catcagtgat
    29161 caaagcagaa tagcttcctg cacaaagtgt tgttgcaaca ttattcgtta gtccattact
    29221 ccataaataa gtatacggag cagtgcctcc cacaacgttt attattcctg ttccgttgca
    29281 aacaccacaa gaggcatcag ttgccgttat tgtacaaatg agtggttgtg gtttggtaat
    29341 cactattgaa catgtacttg tgcaattatt tccggaagta actgttgcag tatacgtacc
    29401 ggctgtaagc cctgtcacag tttgagtagt tgctcctgta gtccataaat atgtgtaaat
    29461 tccggtgcca ccaacagcag acacagttgc tgtagcatta cttcctccat tgcattgaac
    29521 atttacgcct gtaacagagc aactcaagtt attatttgtt actataatta cgacacaagt
    29581 tttagtacat gcatattgat cagaaacagt tacaacataa gtatttggac ataaaccaga
    29641 tatagcctta gtggtagccc cagtactcca tttatatgtg tatgccaaac cgccacccga
    29701 tgcaagcaca cttgcagttc cattacatgc accgcaagaa gcgtctgtac tggtaacaaa
    29761 acaatcaact ggcccatgag ctttcaaggt tgcttcacat gatgatgtgc agccgaattt
    29821 atctgttact gttacggtat aagttccacc aaaaaggaaa tttaaaactt gtgttgttgt
    29881 acctgtattc cataaataag tatatggcga agagccacct aaaggaattg cttttaattt
    29941 tccggttggc agcccgcatt tggtagcatt tgatttaaaa acactacaag ttaacaatgg
    30001 tgatcctgtt atagaaacca cgcaagaagt tgtgcaacca tttgcatctg taacaattac
    30061 ggtatacgtt ccggcacata atccagttgc agttgcgttg gtctgagttg gaatagtatt
    30121 ccaattataa acatatgggc tggtgcctcc ggttgcagct acggttgctg ttccgttaca
    30181 tccaccatta catgctgccc ctgttgctgt tgttgagcat tctaaaagtt ggggttctgt
    30241 aattgtaacg ctgcccgata cagtacaacc agcattatca ctaactataa ctgtataaga
    30301 tcctgcattc aatcctgtag caattaaatt agtttgaaca ggaactgagt tccagatata
    30361 ggaataaata cctgagccgc ctgtcacgtt agcaattgca ctgccggcac taccatagca
    30421 cagtggacta gttggaacaa tattcaagtt caaattactt gacaggccaa tggtaacact
    30481 tgaagtagcg gtgcacccat tagcatctgt aacagtaact gtatacgttc cagaacctat
    30541 tgctgaaata atagctccat tttgaccatt agaccaaaga taagtataag aaggggttcc
    30601 gcctgctgtc gatgcacttg cactaccatt ggtatttcca cacgtaggat ttgaacctgt
    30661 agtaatcaat tgtaattgtg aaggctcatt tatgattatc actaatgagg ctgaacaacc
    30721 attattatcg gttgctgtta ccgtataact atttgcaggc aacccggaaa tggaacttgt
    30781 agtttggcca ttgttccata aataagtgta tgggctggta cctccggatg caacaacgct
    30841 tgccacacca tccgaacctc ctttacattt cacatctgct ttcccgcttg taagcatcaa
    30901 tacagcaggg tctgtaatag tagtttggca aaccgtagta caaccgtttg catcatttac
    30961 agttacagta taagttccag ctattaatcc tgacaaagtc gaaccactta ttccattgct
    31021 ccacaaatag gtatatcccg gagtgcctcc tgttaccaca acgtttgctg tcccgttgct
    31081 atttccatta catgaaggag atgatgaatt aattccacaa atcaatgcat tgggttgagt
    31141 tatttgaaca gataatgttt gactacaacc attcgactgt gtaattgtta caatataggt
    31201 atttgcaggc acattgttta tatcctgagt tgtttgtcca ttggaccagg agtaagtgaa
    31261 aggaggtgtt cctcctatta aagaaacatc tattgagcca ttagttccac cgttgcatgt
    31321 tacattaaca agcgtttcgg aagcgaataa ctgagctggt tctgtaattg taactacagc
    31381 cgttccagta cagccctttt tatcagtcac tgttaccgta taattgccag ctgataaatt
    31441 acttgctagg gcattcgttt ggccattact ccaaagatat aaataaggtg tagaacctcc
    31501 actcgcaact acttgtgctg agccatctgc taatccaaag caagacacat tagaaaaatt
    31561 ggaaatatta acaatcaaag aaggaagttg gttaatatta aaagaaaaac tgcctgagca
    31621 tccaatattg tctgtaactg taactgtata cgtacctgcg caaagccctg tagccgaaac
    31681 agatgtaacg ccattagacc ataggtaagt gtaaggtgat gttccaccag caacgccact
    31741 aactgatgct gtgccggtac aaatactgtt gcaatttgtc ggggttttat ttattgttaa
    31801 aatgatagac gaagacgcat taattccagt gttacaagaa gttgtacaac catttgcatc
    31861 tgtaacagta gcggtatagt tgcctgtagc taatgccgaa attgttgcat ttgtttgtcc
    31921 attactccac aaatatgtgt atccacttgt tccacctgtg gcgttaactg tagcactgcc
    31981 attgttaaga ccacaagtag tatttattgg agaaattgaa caggccaaaa ctgcaggttg
    32041 aacaaccaaa acattacaag aagaagtaca tccaacatta tctgtcacta ttacaatata
    32101 tgatccacct gataatcctg ttgcagttga agttgtttgc aaaggtgttg tattccacaa
    32161 ataagtatag cctggtgttc cgccatttac tgcaacttgt gcttcaccat cgttaccggc
    32221 ataacatgta acaggcattg tattagcggc aatacaacta aggttgcctg gttgagttat
    32281 gcttgcatcg caagttgtta tgcatccgtt tgcatctgta acggttactg tatacaatcc
    32341 tgcgctcagc ccagtagctg tagcaccaaa ttgaggagga gttgtactcc atacataaga
    32401 aaatgctgct aacccccccg atgtggttac tgttgccgtt ccatttggaa aaccagtgca
    32461 agttgcattg gtaactgaag tttgacaact cagcggtgaa acaactgcaa tagtagtagc
    32521 acattggctc gtacatcctt tactgtctgt aattaatact gtgtaactac cacttgaaag
    32581 acccgtggcc gttatattag tttggccatc gctccataga tatgcgtaag gcgatgttcc
    32641 tcctgttaca gaagctgttg cagtaccatt agccgatcca tcgcaactta atgaagtaga
    32701 gatagcaaca caagttaata gggcaggctg tgtgatgaca ataaagcaag atgttgtaca
    32761 accgtttgca tcagtaacaa ttacagtata agtaccggca tttacagaat taatggatga
    32821 tgtagttgcc gctgtagaag tattccacag atatgtataa ggggcaacac ctccatttac
    32881 tgtgactgtt gctgtgccat catttccgcc attgcaactc acattaatac caaatacgct
    32941 gcaacttaaa gcatttgaag atggcacagt tactgaacaa gaagatgaac aacctttgct
    33001 atcggaaatt accaccacat aagttcctga agaaagaccg gtagcgattg ccgtagtttg
    33061 aacaggtgcc gtgagccaaa tataagtata aggagctaca cctcccgaag caacagcctg
    33121 tgctttacca ttattattat tgcaagtggc aatatttaaa acatttgcat tacaaactaa
    33181 ttttgaaggc tgagtaattg ttacgaaaca atttttggta cacccttttg aatcagttat
    33241 ggttactgta tataaacctg ccgcaacacc tgcattatta gcagtgctta caccatttga
    33301 ccataaataa atgtatggcg aagtacctcc ggttgcagtt actgttgccg aaccattact
    33361 tccaccatta catgttacat ttgtttttgt tattgaacaa tttaacactg caggttgggt
    33421 aactgcaatg ctggcattta aaacacaccc ttttgaatca ataattgtaa gcacatagtt
    33481 gcctgctcca atattattta aatcttgagt agtagctcca tttgaccatc cataggtata
    33541 aggtgatgtt ccgcctacca cactcacatc tatagaccca ttagtattgc cgttgcacaa
    33601 tggtctaaca acagtataca atgcagctat agcagaagcc tgtgttattg ttacactaca
    33661 agtagagcta caaccaacat tgtcagttac agaaacagta tagacacctg ttgcaaggtt
    33721 tataactgtt tgagttgttt ttccattgct ccataaatag gtatatggtg cagttcctcc
    33781 attaacagca gcactagcgg aaccatttgt atttccaaaa caagttatgt ttgtgttggt
    33841 aatagaacag ctcacaccaa gttgaggttg actaatagtg atttgtgcac ttgctgtaca
    33901 tccatttgcg tccgtaacaa taccagtata cgtaccagcc atcaatccgg tagctaaaga
    33961 agtagttgta gtaggaaccg tgttccaaga gtaagtataa ggtgctacac caccaaatcc
    34021 attaacacaa gctgtaccat ttgagcctcc attacaagtt acattttgca cacaacccat
    34081 taatgcaaat agttgatttg gttgagtaat tgttacagaa ccaaccttta agcaaccatt
    34141 agcatcagta atagttaccg tatatgttcc ggctgtaagg cctattatgg tttgagttgt
    34201 ttgaccattt gaccaaacat aagaatatgg tgctaatcct ccaattggta gcgctgtgac
    34261 agaaccattg ttgcctccaa agcaagaaac atttatgaca cttgcattgc atatacatga
    34321 aagcgcactt ggttgggtaa tgctaaccga tgcagtacct gaacaaccat tattatcagt
    34381 cactgttaaa gtataaatac caggaccgat attagttaag ttaattgtag tagaaccatt
    34441 actccacaaa tagccaaacg gtgccaagcc tcctccaatg gaagaaataa ttgttccatt
    34501 agaacttcca ttacaagtta cattggtagc agaaagatta actgaaagac ccggtagctg
    34561 actcacagta taactacaag aattagtaca gcctgaaaca tcggtaacag taactgtata
    34621 cgtacctaat gctaaaccgc tgttggtaga agtcatattg ccgttggacc acaaataggt
    34681 atacggtgag gtaccgccac tggtatttaa agtaatactt ccattgttgt ttccggagca
    34741 agttatagga actactgtag cattacaagc taatggtaaa ggattcaaaa cactataggt
    34801 aaattgtgct gagcatccat tagcatcaaa aacaataact gtataggtgc ctcctgcaac
    34861 atttaacaag tttgctgtag tttgagttgg ggatgaattc cataaatatg agaagggtgc
    34921 caatccacct gatattgatg tttgaagaat ctttccgtct gtagaagctg gacaggtagg
    34981 attcacaacc gtataaacag cattgatttg agaaggagaa gaaagcagaa tactggcaga
    35041 tgaagtgcaa ccattgaagt ctgtaacagt aacagtatac gtatttgcac ttaaattaga
    35101 ggctgtttgt gtggtttgac catcgctcca taaataagta taaggcccaa cgcctccaac
    35161 agggtctgca gttacttctc catcacttgc accaaaacaa cttacatcag ttccaatcat
    35221 tgagataaaa attggatctg gttctgttaa agtcacaata acattcttga tacaatttag
    35281 cgagtcgata atagttgcag tataggtacc tcctcctaat cctgttgctt gaaatcccgt
    35341 ttgaggtggt atggtattcc aaactatgtt atatgcatct cctacggtga aaggttcacc
    35401 tccaattaca ctaagagtga taattccgtt gtttgcacca ttacaagtaa ttgccgacac
    35461 attagtagaa gccaccaata aagttggttc tttaaccaat atgcttatag ttgtatcgca
    35521 acctaatgaa tcggtgatag tcaatgacca tattccggct ggcaaattgg tggctaaagc
    35581 tgttgtctga gcaggaactg tattccaaag ataattatat ccataataaa acggagtagc
    35641 accatttacc tgaacaaatg ctgttccgtc agttgctcca tggcatgaaa cagacgtaca
    35701 acaattgttc aatccaatgg cataacgagg ttgttctaaa acatcaaaag ccgaaacaaa
    35761 ttcacagcca ttagcatctg taatagttac cgtataaaca tcagcaacca catttactaa
    35821 atcctcattg gtagtgccat cgctccaagc aaatatataa ggtaatgttc caccaacaac
    35881 cgtgatatca acagagccac tattttctcc ccagcaatta atattattta caagttcatt
    35941 actaatggtg aggtaatctg gttcgtaaat agtataatct tcttgataag tacattgctc
    36001 taaaagatca ttaacataaa cagtatatac ccctgctgtc aatcctgtaa atagtcctga
    36061 agattgccag ctcgaattat caagagaata ttcataaggt cctaccgaat ttccatttgt
    36121 taaccctgtt acttcaatac ttccgtctgc aaatccaaaa caagaaacac tatcaattgt
    36181 aaccaggctc aaagaaataa tagcaaaatc atcagctaca gtggcactac atgtttgcga
    36241 acatcctgaa ttgatatccg taactactac agtgtaagtg cctgcagtta agttattttg
    36301 tgatgaattc gtatcgccat tactccataa aaagtcggca ctaacagcat ttgttaatac
    36361 actaactgtt ccatcaggta ccgaacaatt tgttctgtca gtggaaccac aagtagcggt
    36421 aacaggtatt acagcatcat taactgtagt agcacatgtt ccaatacaac cggacaaagc
    36481 atctgtaacg gtaacagtat acattccagc cgataagccg gatattgcat ttgttgtatt
    36541 tccattgctc cacaagaaag tagcattaag tgagtttgtt attacgtttg ctgtgccatt
    36601 tggagcaata catgagttaa catctgttga ggagcaagtt gcggaaattc ctgtagaatt
    36661 agtgcctaca atagcttcgc aagtagctgt acaaccattt gtattatcgg ttacagtaac
    36721 cgaatatgta cctccggcta aaccacttat tgaagcggta gtttgcccat tactccaaat
    36781 atatgttgga tttagtgcat tggttgttac gctagccgaa ccattttgtg ttgtacaacc
    36841 ggtgttatcg gtggctgtac aatttgcagt agggattgcc gcaggagtat ttaaaatttc
    36901 taatgaacat gttgcagaac agcctgttaa catttcagta actgttactg tgtatacacc
    36961 aggagccaat ccagataggc tttgagttgt ttgaccatta ctccatatat atgttggaga
    37021 taccaaatta ttattaaccg tgcattgacc attaaatata aaacaatgtg tattattttg
    37081 aatcacctgg caagtaacag ctggtgtaga ttgattgaca accaacacag actggcaact
    37141 tgctgtgcaa cccgtagtta aatc
//
LOCUS       DAORQA010000010        36678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_237_length_36678_cov_30.937826, whole genome shotgun sequence.
ACCESSION   DAORQA010000010 DAORQA010000000
VERSION     DAORQA010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 36678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36678
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_237_length_36678_cov_30.937826"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2542
                     /locus_tag="PK736_01815"
     CDS             <1..2542
                     /locus_tag="PK736_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32160.1"
                     /translation="TVELPTQFKPLGLHIAALDNNKNLWLLKNKKALLKYNIQTQQWT
                     VYDSTICPIVNLGVKDLVFDNQNNLWALSPVSGLFKFDGTTWFHYDSLNGGLTTNLIL
                     CLAIQNNTLWIGTKSGALYGTPGNWNVFNKFNSGIAGDTISKIIIDDTKNRVWFRSDY
                     NFGLSEYDNGNWKQIDTIPDVNYFYADNMVCDNGLLYFTIGQYQLYTYDGVSVVRNFS
                     IDEPDFVQLNNSVYFKNAIDGQIYAIGNYLVIVDSLHRNSYFDARFKLSTMVVLNNKI
                     YFAYKDSLYIFDPSLYMQPLVDNIFNPGQIGKYLNKGKVKTAILNNGTNNWDMNNAQY
                     EVPKGSDKHAIYNTGLWIGGLDDNNNIHVAAQTYRQTGVDYWTGPLDTIDASIDSATS
                     ATYNNIWKIDRYDVEEFKHMFANGQVQNGNYIIPTDILTWPAQGIKNKAKNLAPFIDT
                     NNDGKYDPTDGDYPKIKGDQMLWYILNDNLAPHTESNGGNPLGVEIHCSAYAYNCDTL
                     PDSLDVINTTTFYEYKFINRSDTNYHDVYTGLYTDVDLGFATDDFIGCDSTNNIAFGY
                     NGDNFDDGGFGYGANPPMINIQVLQGPNPSLNDGKDNNHNGLVDEQNERCMMEHVMYI
                     CGFFICENPYSVTDYYRYMKGEWLDGRHLTYGEDGMSLTNPPTNFMYSGTPYDSTGWT
                     EINENHAPEDRRFMLSSGPVNLPAHSEQTIDYAIVYTREPNAPNGLHTSVARNIADVQ
                     KIKKWFDTNTFPSCNKYEFPVFPSQIPPLDSGLIIFPNPAIYNFTLNINDDFINASTI
                     KIYSALGQKIYDKNISTKTINIETWHRGLYFVVVEKEGELYKGRFLKW"
     gene            3139..3879
                     /locus_tag="PK736_01820"
     CDS             3139..3879
                     /locus_tag="PK736_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC32161.1"
                     /translation="MILGKKLVIVLPAYNASLTLRQTYSEIPFDIVDEVVLVDDASKD
                     ATAQLANELGIRHVIVHEQNKGYGGNQKSCYDKALSLNADIVIMLHPDYQYTPKLIAA
                     MASIIANDVYKVVLGSRILGQGALKGGMPVYKYIFNRFLTLSQNILMNQKLSEYHTGY
                     RAFDSEVLRKINYHANSDDFVFDNQMLAQIFFAGYDIAEVTCPTKYFDEASSINFSRS
                     MKYGLGVLGVSFQYFFTKLGMMKAKIFR"
     gene            3962..6211
                     /locus_tag="PK736_01825"
     CDS             3962..6211
                     /locus_tag="PK736_01825"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRC32162.1"
                     /translation="MSEALEYHSSGRPGKIEVIPTKPSSTQRDLSLAYSPGVAEPCLA
                     IAANVDDVYKYTSKGNLVAVISNGTAVLGLGNIGPHASKPVMEGKGILFKIFADIDVF
                     DIEIDQENVDDFVKTVKAIAPTFGGINLEDIKAPECFEIERRLKEELKIPVMHDDQHG
                     TAIISAAALINALELCGKKIEDVRIIINGAGAAAISCGKLYISLGAKQENIVMLDSKG
                     VIRADHEITDHNKKHFATALDLNSLDDAMVNSDVFIGLSKGNVLSRSMVASMNAQPIV
                     FALANPEPEINYEDAMAARKDLIFATGRSDYPNQVNNAIGFPFIFRGALDVRATQINE
                     AMKLAAVYAIAELAKETVPEIVNIAYNKKNLSFGREYIIPKPLDPRLISTVAPAVAKA
                     AIKSGVAQTRIANWEQYAEELDARIGRDDKLSKVLISLAKQNPQRVVFGEAENYKMLK
                     AAQIVNDEGIAKPILLGNKQKIDKLIKENNLHLGDIVVIDPREDETYHQQFGEALYEK
                     RKRKGLTIYDSKKMMRERNYYGAMMVETGQADALISGLTRQYSDTIKPALRILGKQDD
                     TRIVAGMHIIMTKRGPIFFADTTVNLNPGWEELVDITLLVAKAMQQFNLKPRIALLSY
                     SNFGSSDDPNVRKVRQAVNYLHENYPSLVVDGEIQANMAFNTQLLKDNYPFTELVNGG
                     ANALVFPSLEAGNIAYKLMMELAGYEAIGPILLGMKKPVHVLQLGSSIREIVNMVTIA
                     VVDAQSRKV"
     gene            6328..6921
                     /gene="ruvA"
                     /locus_tag="PK736_01830"
     CDS             6328..6921
                     /gene="ruvA"
                     /locus_tag="PK736_01830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRC32163.1"
                     /translation="MYEYFNGKFAKVTATQVVVDVNGVGYDINISLNTFAKIKELHEG
                     KIYVHLSIKEDAHTLYGFADDDERKMFRHLITVNGVGTNTARMILSSLSPNELQMVIG
                     GGNVAVLQKVKGIGAKTAQKIIIDLKDKVIKDGPSGSTGAGTIFVTSSARSEALSALV
                     TLGFARNVAEKAIDTTIKQKGDNLNVEELIKGALGNL"
     gene            7086..7631
                     /locus_tag="PK736_01835"
     CDS             7086..7631
                     /locus_tag="PK736_01835"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRC32164.1"
                     /translation="MKIYTKTGDKGQTSLIGGTRVMKHHVRIEAYGTIDELNSWIGLL
                     QSTLPSSAVKIMPEIQDRLFTIGSLLAADPEKSRMTLPQLEQADILLLENEIDAMNDV
                     LPAMRSFVLPGGSIGVSQVHIARCVCRRAERCITTLMEHAEINNDILVYVNRLSDYLF
                     VLSRRVGQLENAAEIPWKPRV"
     gene            7786..8007
                     /locus_tag="PK736_01840"
     CDS             7786..8007
                     /locus_tag="PK736_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HRC32165.1"
                     /translation="MYWTLELASHLEDAPWPATKEELIDYAVRSGAPMEVIENLQELE
                     DEGETYENIDEIWPDYPTKDDFFFNEEEY"
     gene            8238..9497
                     /locus_tag="PK736_01845"
     CDS             8238..9497
                     /locus_tag="PK736_01845"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC32166.1"
                     /translation="MNLVTHKAFAAWRYANFRLYMVARLTLTMGIIMQSVITGWMVYL
                     YTKDAFMLGLTGLAEAIPAIAVGLYAGYLADKYHRKKIILTSLLFLILSSVCITYINF
                     HEHYYYTKFGVVPLFAFTFCVGISRGFLASATNAMSAGLVPLEVLPNMISWNTTAWYI
                     GAIAGPAIGGIVYNNYNAGYVSLLYTLLMVIAFVSYVFLKIPAATHTLTQSWFLSFKE
                     GVVYLKQNKILLGAITLDLLAVMFGDAIVLLPAFAATVLNIDAATTGWLRAAPAVGSI
                     VMATWLAFHPPVNNTGRKLLFSVACFGFAMIAFGLSDSFMLTYIILALAGMFDTVSVI
                     VRNIILQTTTHDSMRGRVASINSIFIGSSNEIGAFVAGSMAKMFTLQASVVLGGCITV
                     FVVLLMWRIVPELKKYNQLEKSQNVSE"
     gene            9810..10223
                     /locus_tag="PK736_01850"
     CDS             9810..10223
                     /locus_tag="PK736_01850"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HRC32167.1"
                     /translation="MKSVIEKKIWVIAGVIFCMIGVVGVILPLMPGLVFFIIASYCFA
                     RGSRKFLRILLSNKTIGPQIMDYKRGKGMTMKTKVIAIAMVLVSMSISAFVMVKIYWV
                     KWAICVTCIGVVAIIFKQKTRIAETPLNGIVKTNS"
     gene            10377..10949
                     /locus_tag="PK736_01855"
     CDS             10377..10949
                     /locus_tag="PK736_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32168.1"
                     /translation="MILAVSLCGSLKAQPLLIENFTGTQFNNSIVLNFTLVKGNTCTD
                     TEIQRSTDGINFIVINTIAGICGSTQENISYSITDNSPVANSYNYYRINLRSLGFSDI
                     IKVRYIDFSASNALVIYNSQQQLSTLFYKNPSAKKIEFEILNQHGMKIYSGTSNTSEW
                     NVSTSAWPRGLYLVKFMNGDVAITRKLMVF"
     gene            11260..11481
                     /locus_tag="PK736_01860"
     CDS             11260..11481
                     /locus_tag="PK736_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32169.1"
                     /translation="MVEFKIELDENFVKTFGHKQIENYLEEFMQKIVIRLSAKDILKD
                     LETVDLANDKEWQVSRNLAWQQEKHKYFA"
     gene            11484..11906
                     /locus_tag="PK736_01865"
     CDS             11484..11906
                     /locus_tag="PK736_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRC32170.1"
                     /translation="MTDFVIDANVLMSVLISGKSSYRPFLTYYNFIVPEFVLVELEKY
                     NDVLKSRTKMQQFEFVQWTYFVFSQLTILPQYVLSHESLEKSKTLLNKLDLKDMTYVA
                     LAIQLDLPLLTRDKPLYEGLKKQKFRKIILFEDFLKTL"
     gene            complement(12363..13928)
                     /locus_tag="PK736_01870"
     CDS             complement(12363..13928)
                     /locus_tag="PK736_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRC32171.1"
                     /translation="MTIAAAVKAEYGIAEVLKKLGIDKMNSGACTGTQWFDTKGEVID
                     SYSSSDGELIATLHQATTADYDTIVKTSQSAFKQWQMIPAPKRGEVVRQIGNVLREYK
                     EPLGKLVSYEMGKIYQEGLGEVQEMIDICDFAVGLSRQLHGFTMHSERPKHRMYEQYH
                     PMGIVGVISAFNFPVAVWAWNAMLAMVCGDTVIWKPSSKTMLCAIAVHKIIAQVLKEN
                     NVPEGVLCLVAAGSKYVGDNMLADKRIPMISATGSTRVGKRVGAIVGERLGKSILELG
                     GNNAIIITENADLNIAFQAVLFGAVGTAGQRCTSTRRLFIHKSIYDTFKTKLVQAYGN
                     IRIGHPLDANTLVGPLIDKGAVNDYLSAIEKAKAQGAKIIYGGDVCKGEGYESGCYVL
                     PTIVEAKNEYHIVQEETFAPILYIMPYDTIDEAIQMQNDVPQGLSSSIFTRNLLESEK
                     FLSHAGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDVWKYYMRRQTNTINYS
                     TELPLAQGIKFDL"
     gene            14394..15692
                     /locus_tag="PK736_01875"
     CDS             14394..15692
                     /locus_tag="PK736_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRC32172.1"
                     /translation="MPFNRRHFLKQISAISIGSVFNAQSFAKDLQYFDSKFIDADSNT
                     IAQDEEYWQWVRSNYTINPSLINLNNGGVSPQPKSVQDAFKRYCDLCNEGPSFFMWRT
                     LDTGREPLRQRIADVAGCLSEEIAIHRNTTEALANIVFGIELQPGDEVVLTHQDYPNM
                     VHAWKQRELRQKIKLVWLNLDLPIDDDDAITKTFTQAFTSKTKVVHITHVMNWTGQIL
                     PVRKIADEAKKRGIQVVVDGAHSFAQLDFRIDELNCDYFGTSLHKWMGAPFGTGFMYV
                     RKEKISSLFSLMPNDKPNGDDIRKFEALGTRNFAAEQAVMTAIDFHEAIGIKRKQARL
                     LLLKNYWTQKVKHLPQLKFNTTLNDKYSNAITNVIMDGMSAQEMEAFLYDKYKIHTSS
                     VNWANIHGLRITPSVYTSLNELDKLVHGFELMAEKINVKK"
     gene            15739..16443
                     /locus_tag="PK736_01880"
     CDS             15739..16443
                     /locus_tag="PK736_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRC32173.1"
                     /translation="MLEKLQQYTTAFGNIVFPNGCFMCDRNLIKGEGVICTYCKISLP
                     KTHFQIIEENAVIKHLWGKANIVAATAFLHFIKGSGVQEAIHQLKYKGKKQVGVVLGE
                     WFGYDLIQSEPYASADFLVPVPLHSARFHSRGYNQSEMIANGMSKAMNIPVEKSLLQR
                     GVETQTQTRKHRYERFENMKDVFLHNDDEKFHNKKYLIIDDVLTTGSTIAACAEVLNL
                     LPGAKVMVATLAFANK"
     gene            16587..17321
                     /locus_tag="PK736_01885"
     CDS             16587..17321
                     /locus_tag="PK736_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32174.1"
                     /translation="MLRKISVFILFVFVAQMATSQTVEVLNKPNKRNLTTGYYFDCLH
                     VQIDTTKLVFMATLKATGEKNEGYISALYTAVKQRANELGANCFSLRSYTCLDDSEKA
                     VLIVDAYYANDSILTLNENLHAKNIIYIFSTEKWNDALEYNFKINENVKSLHASEYCE
                     YKLSADEKATLYFGKNFIIKEEFEYHENREAKFVFVPYRGSKLKPEVFIFSNGIYFGV
                     QSKRNKLSHIENSLGWLLVCIFHKTQ"
     gene            17466..17963
                     /locus_tag="PK736_01890"
     CDS             17466..17963
                     /locus_tag="PK736_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRC32175.1"
                     /translation="MSQDILINNSLTKAEKYAELIPQLKSLVDGEDNAVANIANIMAA
                     LKYTMNFFWVGVYWVDDKELVLGPFQGPVACTRIAYGKGVCGTSWQKSETIIVDDVDA
                     FPGHIACSSASKSEIVVPIFYQQKVIGVLDVDSDQLSTFDNDDAEALQHVCDILSSVH
                     SGNIK"
     gene            17972..19135
                     /locus_tag="PK736_01895"
     CDS             17972..19135
                     /locus_tag="PK736_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRC32176.1"
                     /translation="MNRFIKLLAVTLTFVYGYSYSQELTNKKGSLLKFKVVKEVTATT
                     VKNQQSSATCWCFSTNSLLESELMRMGKGSIDIAEMFTIRNMYLLKGKHYLRYMGKAK
                     FGDGGEPHDVMAAIRDYGMLPQSAYNVVPGNNDRIATGEMESVLKGMLDNMLALKDGK
                     LSPVAFKAYSAALDAYLGAPPTEFEYNNKKYTPRTFADEMGIKPDDYIEITSFTHHPF
                     YSKFMLEVPDNWMNGMVYNVPLDELQKIADNAINNGFSMAWSSDVSEKGFSYKNGLAI
                     VAETEFEKMTQDEKDSMFIKPITEKIITQQMRQDAFDNLSTQDDHGMHITGIATDQAG
                     KQYFIVKNSWGTEGNDCNGYFYCSNAYFQFKTTAIMVHKSAIPADIAKKMGIR"
     gene            19768..21813
                     /locus_tag="PK736_01900"
     CDS             19768..21813
                     /locus_tag="PK736_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRC32177.1"
                     /translation="MKKLLAIYFLLFAFIQNLNAQQVQGGQFEFKHTECLNEQQREKI
                     FIDLEQSRQSLIAQGLLQAVNPNAKMLTLFDWPLKQAAGINDPGYYSISNYVDQDNTA
                     NILDYNCGNITYNGHHGTDIFTWPFWWYKMDNNQVEIISAAAGTILYKSNGNFDRSCA
                     SNNNNWNAVYVQHSDGSVAWYGHMKNNSLTTKAIGQTVVAGEYLGVLGSSGNSTGPHL
                     HFEVYNPSNVLVDPFSGACNSLNALTWWNNQLPYKQSGLNKIMTHSTPPVFPACPTTE
                     TPNAKNEFCAGNTVYFSAYYRNQQNGQVLTHKIYRPDNSIFQQWTQTFTTFYQASWWY
                     YSWTLPGNAPSGNWRYEIAYNGSTYNHTFTVGPPVVVVTPPGPVTLCAGSSVTIISSA
                     GTGNIWSNGATTQSITVNTAGTYTVSVSNACGTATSTPVVVNTVALPTATISTNTPTT
                     FCSGGSVTLNANTGVGLTYVWKKNGVVIANATASTYVANKTGNYRVVVTNANGCSKTS
                     AAKVVTVNGLPTATVTPAGPIAFCAGDSAVLSANQGIGLTHQWKKFSNNINGATSFSY
                     TAKTASTYKVVVTNANGCVRTSNAVVVSVNCRLFAATNNNSIQYDAQSNSIIVIAEVD
                     EVTDGTLSIWSMDGRKIIELSAMPNEMTKLKPELKSGVYFVQFQNDEQTISSKIIVQ"
     gene            complement(21861..24182)
                     /gene="rnr"
                     /locus_tag="PK736_01905"
     CDS             complement(21861..24182)
                     /gene="rnr"
                     /locus_tag="PK736_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRC32178.1"
                     /translation="MKLKPKTKQLTPAQNVALAQSLQQIFKMHVTKAFNYKQLAKALK
                     YEDEYFAAQIFEHNYTEEDLRQGIVDCLQKMCAAKIIAETDKGKYKIVPVEVLVEGTI
                     DFSASGSAYVKNADFEQDIYISPNNTLNALNGDTVKISLFAHRANRRAEGEVVEVVKR
                     ARTEFVGTVHIQNKIAFLIPSGVKNNTDIFILPGKLHGAKDGEKAVAKIVEWLPDAKN
                     PAGEIVRVLGMPGNHSVEMDAILVEYGFPLSFDKEVEDEADKIPLDISAKEIKARRDM
                     RGITTFTIDPEDAKDFDDALSIQKLPNGNYEIGIHIADVSHYVVPGTALDKEAYSRAT
                     SIYLVDRVIPMLPEKLSNHVCSLRPHEDKLCFSAVFEMTEAGELINEWFGRTVIHSDN
                     RFSYEQAQQVLEDKKGTLATELLLLNKIAKALRKQRFINGSINFDKVEVKFKLDEEKM
                     PVGVFLKVMKDSNQLIEEFMLLANKRVATFVGKYSAKYLLNTEGKTKDVAGHKTFVYR
                     IHDVPDPEKIKTFAQFAAKFGYKMDVQSEKNISKSINKLIKDVAGKPEQNLLETLAIR
                     SMAKAKYDTNNIGHYGLAFDYYSHFTSPIRRYPDVLAHRLLQYYLDGKASEPVAYYAQ
                     ACKHSSAMEVQATEAERASIKFKQVQYLQARMTEVFDGIVSGVSEWGMFVELVDSKCE
                     GMIRLRDLNDDYYELDEKNYCLRGSRSGNTFQLGDKLQVMVKNTDLQRKQIDFMVYDE
                     DAPMRARTERQPSNFSHPKRNQKYSGKKSKKRR"
     gene            24341..25792
                     /locus_tag="PK736_01910"
     CDS             24341..25792
                     /locus_tag="PK736_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRC32179.1"
                     /translation="MKAGINSIKIGANKFIAIAILIICSHTVNAQLTIILDSVPRYYT
                     PLFDSVYIGGSFNNWAIGSAAYALLPNANGKPQITLNDTGTIQFKFAHGGWASVEVNS
                     SGGYVPNRTYTFTGNADTIHITIPNWADSYTASTHTVTNTVYVVQDNMWMPQLARYRR
                     VWIYLPNDYYTSNNYYRTVYMMDGQNVFDAATSFAGEWYVDDSLKVAQLAGDSGAVVV
                     AIDNGGQYRLDEYCPFINTQYGGGQGDLFTDFLVNTLKPVIDANFRTYTDREHTAIAG
                     SSVGAYIALYAGIKHQAVFSKVGIFSPAYWFQDSIYTFVKNSTAQFPMRFYQVCSVNE
                     GGSVVYNMNRMRDTLIASGFSSGDIYNISRSYGSHSETFWRKEFIPAYTWLFSNSIGN
                     YFIEYMHSDKLSVYPNPADTFISIVHHLKKEEIKSIRIFNVEGKEQFTASNNLESQDY
                     KSTISINLTTGVYVVWILDNNDKLFTKKFVVRR"
     gene            complement(25936..27399)
                     /locus_tag="PK736_01915"
     CDS             complement(25936..27399)
                     /locus_tag="PK736_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32180.1"
                     /translation="MHAQLPYNKNAVMVSAGSLVSSNFKIKNGTGDLNLINPIRLNTT
                     QFGAGIKKFYTKDFFTEFHFLYGWQNFGLASNISSKNYPQINTGWNNFVSAFGKSYVS
                     LEFFPSMEFRFKRNYCLNLLGGLGFKYFDKVGLSIGANYDNELDSDSSLYPFFSFSTY
                     SLEKRPYHLTALFKAELLKQFKSHHQIGLGIKYQLGLQNMFEGSYVFFEGQKDESRGD
                     WYFRGSQLGAYLTYQYLYNANKKPREKNYVKPVYTYVTRRNQFYLEASHNWVFSPQYK
                     NIIGNIKLEPWQMWTTSFGLGYTRMLNNKWGLNLTAIMNLDKLGGRVALKKQDYIDVI
                     ARDIDRTDYVTKLDPALKFGATYHYQMRNNYSLSFSSGLSIRYFYPEFYTLLFGDYGI
                     GNTFNYLLIVDHDFYYEPEPRLLKGLYFEPSISKQLKKKNMITVGVNFTYSFQKIIDA
                     EYHILPDTKYHTVGRYTVDGHQLGAFVRYTLSGKRAK"
     gene            complement(27498..28520)
                     /locus_tag="PK736_01920"
     CDS             complement(27498..28520)
                     /locus_tag="PK736_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32181.1"
                     /translation="MRKVLVLLTIIALASACNKEKDIKPQTANSIASIASKEGDGSDC
                     VFNVTVCNGTFIFANQAEYDVVYNCLEQEYEQHNDDFEALYANLNDDDFNAMADQIGF
                     DEDQTLINFELSHSFVSYRDKLRKLEDVWLNNTTLNPATDPDLIDYYDDAVASALASD
                     AGKVMIGTDLYVIDTQGEMWVLPNATCNDIPGLSNDPAVLANTPQVKTIHGGFPLNDC
                     KNNWRHHGLEPYDSNKQLKWKLKFTATHWGGDGTKAYAKTVSYKKKANGKWKNFKTNL
                     LVKSSGNWTTNCADFTAYSEQKTKRRNRLKAPAYDGGFYRYHKIGEAHGYYEATNYNF
                     NFTKTY"
     gene            28763..29137
                     /locus_tag="PK736_01925"
     CDS             28763..29137
                     /locus_tag="PK736_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC32182.1"
                     /translation="MDKKATKKLDIGFNLKRLREMKGYKPEYIAEHINVAPRTYLDYE
                     NNSHDIGINKLASIAEVLEVTLWDIITFDNKSFWQNNFHNESESKATYTVNNNPPSNK
                     ERELYEKIIANLEATIALMKNK"
     gene            complement(29361..31292)
                     /locus_tag="PK736_01930"
     CDS             complement(29361..31292)
                     /locus_tag="PK736_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRC32183.1"
                     /translation="MTQLKVVLGAVAFSVCAFTQAFSQAPLVVCPTDPIAATIDSLIT
                     IDNVVRFRNINTLNYTATSKEDIYNVPTFPEQVYKDRISKINSPIPYVYNDQVRAYIN
                     VYATQKREGTKRIMGLSSIYLPMFEEVLEREKLPLELKYLAMVESALNPTAVSCAGAT
                     GLWQFMYNTGILYDLNVNSYVDDRRDPYKSTVAACQYFKKMYEIYHDWLLVIASYNCG
                     PGNVNKAIIRAGGKSDFWSISPYLPAETRGYVPAFMGISYVMEYSREHNLIPVPPSIT
                     YFETDTVIVTKKTTFSTLSKNLDIPVDVLGFLNPTFKRQLIPATISQPYALRLPTNKI
                     MAYTQKQTTIEMEDNLLASTQPTINLGKGRTNIALQEEEAKYTYVTKTVKQKYTVRRG
                     ESLARVADKFNCTQGEIKSWNRLRSNKVSRGQHLTLYVDQRVKVLKDDAAALQQTASV
                     KPAVNNETIETEIAVTQPKANAEQLVTKTKKLSYRTQKGESLAVVARKMNCTVNDLKE
                     WNHIKNNTIHPNQTLVVYREVKEIVDPSKPTKIVLATAGIAADDSAINKAKAEEEEIA
                     QQEKSELKVVAKKGKNKTEPKSNNEINYLYYTVRKGDTLYSIAKSKGTTIDNIKKFNN
                     ISSGKQLRQGLKLKVKIIG"
     gene            complement(31428..32105)
                     /gene="rsmI"
                     /locus_tag="PK736_01935"
     CDS             complement(31428..32105)
                     /gene="rsmI"
                     /locus_tag="PK736_01935"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRC32184.1"
                     /translation="MSQSKLILVPTPIGNLDDITLRAIKVLQQCDLVLAEDTRVSRFL
                     LKHLGIEKKIQSFHSNNEHHVLERTVNLIRENNMVALVSDAGTPAISDPGFLLVRACI
                     AANIDIETLPGPTAFVPALVNSGFPCDRFCFEGFLPHKKGRVTRITDWLTEERTIILY
                     ESPHRLIKTLQQMNDILGPDREVCVCRELTKMYEEVKRGKISDLLLHYTNTPPKGEIV
                     MLLRGCS"
     gene            complement(32447..33202)
                     /locus_tag="PK736_01940"
     CDS             complement(32447..33202)
                     /locus_tag="PK736_01940"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRC32185.1"
                     /translation="MFTKINFNNKTYSAQLNDCIDISIPLRAGADNVNAWWAAPVTIE
                     AVRTETFIGDVNQGGSVNFRNVALNPHGNGTHTECVGHISKEPYTINQCLKHFFFVAQ
                     LITVTPSETGNRDKVILSESISKVLMPNVDAVILRTLPNDNSKLTRQYSGTNFAYVQN
                     EVMEIMVKNNVQHFLIDLPSVDREFDDGKLQAHHTFWQYPHATRTQCTITEMVYIENK
                     IEDGIYLLNLQITALENDATPSKPVLFRMVELS"
     gene            complement(33461..35242)
                     /locus_tag="PK736_01945"
     CDS             complement(33461..35242)
                     /locus_tag="PK736_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRC32186.1"
                     /translation="MTKKTLLVLAIAIGCLHNDGSAQSWSLTGNTGTSASTNFIGTKD
                     AVTFKIRTNNITRVFVNASGTVAIGNSAPRYPFDVKVGSINTDSLYRIGGNRALYSTL
                     NTNLMVGNAGTLGISGSGNTLVGEGAGTSITTQSNNTFVGRSVGGICTSNNNTVMGRS
                     AAYTLSTGVNNCYFGMNSGYNANTGSANVAMGAYTLYFNSGKSGLVAIGDSALFNNSI
                     GGGAQAYLSSDNTAVGQKALFSNNSGFLNTAIGSNALRNNTSGFSNTAVGVYAMAGCN
                     TGFYNTVSGYSAFQSLTTGSRNVSLGYFTGISITTGTNNSYVGTYATGSFSTITNSSA
                     FGYDASVDASNKVRVGNTSVSSIGGQVGFTNFSDARVKNNIQSNVPGLAFIKLLNPVT
                     YHFDIRKENELMGITEKDDWEGKYDIEKIQFTGFLAQDVDAAAAKINYDFSGVDKSGK
                     LLGLRYSEFVVPIVKSVQELSTQNEMLQAQINKQQIQLDEMRNMIATMKGDNNLLNNT
                     QGQWMKQNEPNPFNQTSTINFNIDAKVSSATIVISAANGDVIKTVNVNTTGSLQLNAS
                     EFAAGQYFYTLMVDGKAVEKKSFTVAH"
     gene            complement(35447..>36678)
                     /locus_tag="PK736_01950"
     CDS             complement(35447..>36678)
                     /locus_tag="PK736_01950"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HRC32187.1"
                     /translation="CPHCKELIDVNDVLYHQLQEQIGKEFKTKEAAAQKILREKEMMI
                     EQQQLKMAEEKAAMENAIAKSVSEKITAERIKIEQELKVQITNEKAGEMQVYMRELET
                     KREEVKQLNKLKAENEIIKREKDELRDKITFEKEKELSHKLNEEKAKIKQQAEEENVL
                     KLREREKIIDDLKQQVETIKRKAEQGSMQMQGEVQELELENILRETYIYDDINEIKKG
                     QRGADILQTVRTRQGVECGKIYYESKRAKNFDNNWIVKLKEDNLEVKADQLVLVTETL
                     PDGYTKFFVKDGVWICTLWEVKGLSLVLRYMMYELHTTRTALSGKESKMELLYEYLTG
                     HEFRGQFEAIIEGFSDLQKSYMDEKMKMMKIWKEREKQFERIMTNSINFYGSLKGIAG
                     SSIQDIEMLEGKKEKLL"
BASE COUNT        11119 a         6967 c         6955 g        11637 t
ORIGIN      
        1 aaccgttgaa ttgccaactc aatttaaacc cctcggactg catattgctg cacttgataa
       61 caataaaaat ttatggctat tgaaaaacaa aaaagcttta ttaaaatata atattcaaac
      121 tcagcaatgg acggtttatg atagtacaat ttgcccaatt gtgaatctgg gggtaaaaga
      181 tcttgttttc gacaaccaaa ataacttgtg ggcattatcc ccagtaagcg gcctatttaa
      241 attcgatggt actacttggt ttcattacga ttcattgaat ggtggattaa caacgaatct
      301 gattttgtgc ttggcaattc aaaataatac gctttggatt ggaacaaaaa gtggagcttt
      361 atatggcaca ccaggtaatt ggaatgtgtt taataaattt aactcgggaa tagctggcga
      421 cacaattagt aaaataatta ttgacgatac aaaaaaccgg gtttggttta gatcggatta
      481 taattttggt ctgagcgaat atgataatgg caattggaaa caaatagata caatacctga
      541 tgtcaattat ttttatgcag acaatatggt atgtgataat ggattattat attttaccat
      601 tggccagtat caactttata cttatgatgg tgtgagtgtg gttcgcaatt ttagtattga
      661 tgaacctgat tttgttcaat tgaataattc tgtttatttt aaaaatgcca tagatggtca
      721 aatttacgct ataggtaatt acttggttat tgtagactct ttacatcgta attcatattt
      781 cgatgccagg tttaaattaa gcactatggt agttttgaat aataaaattt atttcgcata
      841 taaagatagc ttatacattt tcgatccttc tttgtacatg caacctcttg ttgataatat
      901 ttttaatccc ggacaaattg gaaaatactt gaacaaagga aaagtaaaaa cagcaatctt
      961 aaataatggc acgaataatt gggacatgaa taacgcacaa tacgaagtgc caaaaggcag
     1021 cgacaagcat gcaatttaca acacagggct gtggattggt ggcttagatg ataacaataa
     1081 cattcatgtt gcagcacaaa cttatcgcca aacgggagtt gattattgga ctgggccatt
     1141 ggatacaatt gatgcatcta ttgattctgc aacatcagct acgtacaata atatttggaa
     1201 aatagatcgc tacgatgttg aagaatttaa acacatgttt gcaaacggac aagtgcaaaa
     1261 tggaaattat ataataccaa ccgatatact cacatggcct gcacaaggca taaaaaataa
     1321 agcaaaaaat cttgcaccat ttatcgatac aaacaacgat ggaaagtatg accctacgga
     1381 tggcgattat ccaaaaataa aaggcgacca aatgttgtgg tatatactaa atgataacct
     1441 tgcacctcat accgaaagca atggtggtaa tccactaggt gtagaaatac attgctccgc
     1501 atatgcttac aattgtgata cattgcccga tagtttggat gtgataaata ccacaacatt
     1561 ttacgaatat aaatttatta atcgcagcga taccaattat catgatgttt atactgggct
     1621 ttatactgat gttgaccttg gctttgccac tgatgatttt atagggtgtg actcaacaaa
     1681 caatattgcc tttggataca atggcgataa ctttgatgat ggtggatttg ggtatggtgc
     1741 aaatccacct atgataaata tacaagtttt acaaggcccc aatccatcac taaatgatgg
     1801 caaagataat aaccacaatg gactagttga tgaacaaaac gaacgttgca tgatggagca
     1861 cgtaatgtat atctgtggat tttttatttg cgaaaaccct tattctgtta ctgattatta
     1921 tagatatatg aaaggtgaat ggttggatgg ccgccattta acttatggag aagatggaat
     1981 gagtcttaca aacccaccta ccaatttcat gtacagcggc accccatacg atagtacagg
     2041 ttggacagaa ataaatgaaa atcacgcacc cgaggataga cggtttatgc tatcctccgg
     2101 cccggtaaac ttacccgccc acagcgaaca aacaatcgat tatgcaatag tttatacacg
     2161 cgagccaaat gcgcccaacg gattgcatac ttcggtagca cgcaatattg ctgatgtgca
     2221 aaaaataaaa aaatggttcg acaccaatac atttccctcg tgcaacaaat atgagttccc
     2281 tgtttttcca tcacaaattc ctccgctcga tagtggtctt attatttttc ctaaccctgc
     2341 tatttacaat tttacactca acataaatga tgattttata aatgcatcta ccataaaaat
     2401 ttatagtgca ctggggcaaa aaatttatga caaaaacatt tctacaaaaa ccatcaatat
     2461 agaaacatgg cacagagggt tgtattttgt agttgtagaa aaagagggcg aattgtataa
     2521 agggagattt ttgaaatggt agttggttgc gttgttattg ggttattgag ttattagatt
     2581 gttgcctttg gcatgctgag tgtcaaagtg agtgttgtca ccctgaacgc agtcgaaggg
     2641 attatcgaac tgcgaagcat ctcttcaact tattggtcat tggtcattgt caccctgagc
     2701 gaagtcgaag ggatcattgg tcattatcgt cattgcgatt tgttacagtg agtattctcg
     2761 atttgagaag caatttcttc caagcattgg tcattttaat tttttgcttt tctaattttt
     2821 aatggcaaaa aaacatttgc attcattttg tgcttttcgt gttttcagtg gcaaaaagta
     2881 tttgcatttt attcggtatt cattcgtcct cttcggggca aaaagcattt tcatttattt
     2941 tgcgcttttc gtgttttcag tggaataaag tattcacatt tattcggcat ttattcgtcc
     3001 tattccccgt taagcggggc aagctgtggc aaaaagcatt cgcattttct ttatccctct
     3061 gcactcataa tttaattaaa aattgatttc acattctata caaaatgatg atgcaacttt
     3121 tcttttcttt gcgtgcttat gatactcgga aaaaaattag taatcgtatt gccggcatat
     3181 aatgcctcgc tcactttgcg acaaacctac agcgaaatac ctttcgatat tgtggatgaa
     3241 gttgtgcttg ttgacgatgc cagtaaggat gccacggcac aacttgccaa cgaattgggt
     3301 attaggcatg tgatagtgca tgaacaaaat aaggggtatg gtggcaatca aaaatcgtgt
     3361 tacgataaag ccctttcgct taatgccgat attgtaatta tgcttcatcc cgattatcaa
     3421 tacacaccaa aacttattgc tgcaatggca agtataattg ccaacgatgt atacaaagta
     3481 gttttgggtt cgcgcatact cgggcaaggt gcactcaaag gtggtatgcc agtttacaaa
     3541 tatattttta accggtttct tacattgtct caaaacatat tgatgaacca aaaattgagc
     3601 gagtatcata ctggttatcg cgcattcgac agcgaagtgt tgcgcaaaat aaattaccac
     3661 gccaacagcg atgattttgt tttcgataat caaatgctgg cacaaatatt ttttgccgga
     3721 tacgacattg ccgaagttac ctgccccact aaatattttg acgaagcttc atccatcaat
     3781 ttttcgcgca gcatgaaata tggcttaggc gttttgggag tatcgttcca atattttttt
     3841 acaaaattgg ggatgatgaa ggcgaagata tttaggtaga aaaacggacc cctccccatc
     3901 tcccaccaaa tgcatatctt gcgacccaca ttttaaaact attcttttca aaaaataaat
     3961 tatgagcgaa gcattagaat accacagcag cgggcgtccc gggaagatag aagtaatacc
     4021 taccaaaccc agcagcacac aacgcgattt atcgttggca tattcgcccg gtgtggccga
     4081 gccttgtttg gccattgctg ccaatgttga cgatgtttat aaatatactt cgaagggaaa
     4141 tcttgttgcc gtaatttcga atggcactgc cgtgttggga ttgggaaata ttggcccaca
     4201 tgcatccaag ccggtgatgg aaggtaaggg aattttgttt aaaatatttg ccgacatcga
     4261 tgtgttcgat atagaaatag atcaggaaaa tgtggacgat tttgtaaaaa ctgtaaaagc
     4321 cattgcacca acttttggtg gtataaattt agaagatata aaagctccgg agtgtttcga
     4381 aattgaacgc aggctgaaag aagaattaaa aattccggtg atgcacgatg accaacatgg
     4441 cacggcaatt atttcggcag cggcacttat caatgcactg gaactttgtg gaaaaaaaat
     4501 tgaggatgta cgcatcatta ttaacggtgc cggtgcagca gcaatatcat gcggcaagtt
     4561 gtatatatcg cttggtgcca aacaggaaaa tattgttatg ctcgatagca aaggagtgat
     4621 acgtgccgac cacgaaataa ccgatcacaa caaaaaacat tttgccacag cgctcgattt
     4681 aaattcgctt gacgatgcca tggtaaacag cgatgtgttt attggattat cgaagggcaa
     4741 tgtattatcg cgcagcatgg ttgcaagtat gaatgcgcaa ccaatagtgt ttgcacttgc
     4801 caatcccgag ccggaaataa attacgaaga tgcaatggca gcgcgcaagg atttaatttt
     4861 tgctacgggc cgcagcgatt atcccaatca ggtaaacaat gctattggtt ttccatttat
     4921 ttttcgtggt gcactcgatg tgcgtgcaac acaaattaac gaggccatga aacttgccgc
     4981 agtgtatgcc attgccgaac tcgcaaaaga aaccgttcca gaaattgtaa acatcgccta
     5041 caataaaaaa aatctcagct tcggtcgcga gtatatcatt cccaagcccc tcgacccgcg
     5101 attaatttct actgtggctc cggcagttgc aaaagctgca ataaaatcgg gtgtggcgca
     5161 aacgcgaatt gccaattggg agcaatatgc cgaagaactc gatgcacgca ttggacgcga
     5221 tgataaatta agtaaggtgc taatatcact tgccaaacaa aatccgcagc gtgttgtatt
     5281 tggcgaagcc gagaattaca aaatgctgaa agccgcccaa atagtaaatg acgaaggtat
     5341 agccaaacct atattgttgg gcaacaaaca gaagattgat aagcttataa aagaaaataa
     5401 tttacacctg ggcgatattg ttgtaataga tccgcgcgag gacgaaacgt atcatcaaca
     5461 atttggcgaa gcattatatg aaaagcgaaa acgtaaagga ttaacaattt acgattcgaa
     5521 aaagatgatg cgcgaacgca attactatgg cgccatgatg gtagaaacag gtcaggccga
     5581 tgcacttata tcgggcctca cccggcaata ttccgacacc attaagccgg cattgcgcat
     5641 tcttggcaag caggacgaca ctcgtatagt tgcgggtatg cacatcataa tgactaaacg
     5701 cggcccgata ttttttgctg atactactgt aaatttaaat cccggatggg aagaacttgt
     5761 cgatattact ctgcttgttg ccaaagccat gcaacaattt aatttgaaac cccgcattgc
     5821 attgttatcg tattcaaact ttggttcgag cgatgatcca aatgtgcgca aggtgcggca
     5881 agcagtaaat tatttacatg aaaattatcc gagtcttgta gtggatggcg aaatacaggc
     5941 aaacatggca ttcaatacac agctgctaaa agacaattat ccttttaccg aattggtaaa
     6001 tggcggtgca aatgcattag tttttccttc gctcgaggct ggcaatattg cttataaact
     6061 gatgatggaa cttgcaggat acgaggccat aggtccaata cttttgggta tgaaaaaacc
     6121 tgtgcacgtt ttgcaactgg gcagcagcat tcgcgaaatt gtaaacatgg taaccattgc
     6181 cgtggtggat gcgcagtcaa ggaaagtttg attttattga aaaaaacgaa acggattttg
     6241 aaataaaata atctcctctc aaatcaaaaa gtaattttat tcgtttaaaa attagaacag
     6301 acaaaatcaa aataatttca gattaacatg tacgaatact tcaatggcaa gtttgcaaaa
     6361 gtgacggcaa cacaagtagt ggtggatgta aatggtgtgg gttacgatat aaatatttcg
     6421 ctcaatacat ttgcaaaaat aaaggaactg cacgagggca aaatatatgt gcatctttct
     6481 ataaaagaag atgcccatac gctttatgga tttgccgatg acgatgaacg caaaatgttt
     6541 cggcatttaa ttactgtaaa tggtgtaggt accaatactg cccgtatgat attatcgtca
     6601 ctttcgccaa acgaattaca aatggtaatt ggcggaggca atgttgctgt attgcaaaaa
     6661 gtaaaaggca taggtgcaaa aacagcacaa aaaataatta tcgatttgaa agataaagta
     6721 attaaagatg gtccatcggg ttctactggt gcaggaacaa tttttgttac aagttcggca
     6781 cgcagcgagg cattgagcgc ccttgttaca ttggggtttg cacgcaatgt tgccgaaaaa
     6841 gcaatcgata ctaccatcaa acaaaaaggc gataatctaa atgtagaaga attgataaag
     6901 ggggcattgg ggaatttgta ataaaaagaa agttttgaaa tttttttaat gccacgaaaa
     6961 acacgaaaca atttgccgaa atgtgtttta tataattgat tgaagtgagg agtaaatgca
     7021 caaattttta atagcagagc aactccaaaa aaaggtacta atgatttaat gtttatttca
     7081 aaaaaatgaa aatatataca aaaacaggcg acaaaggaca aacttctttg ataggcggca
     7141 ccagagttat gaagcaccat gtgaggattg aagcctatgg cactattgat gagcttaatt
     7201 cctggatagg acttctgcaa agcacattgc catcatccgc tgtcaaaata atgcccgaaa
     7261 tacaagaccg cttgtttacc attggttcgt tgcttgcggc cgaccccgaa aaatcgcgca
     7321 tgaccctacc tcaacttgaa caagcggata tattattgct cgaaaatgaa atagacgcta
     7381 tgaatgatgt gttgcccgca atgcgcagtt ttgtgttgcc cggaggcagt attggcgttt
     7441 ctcaagtgca tattgccagg tgtgtatgcc gcagagccga gcgttgcatc accaccttaa
     7501 tggagcatgc cgaaataaac aatgacatac ttgtgtacgt aaacaggctc agcgactacc
     7561 ttttcgtgct ttcgcgcagg gttggccaat tggaaaatgc ggccgaaatt ccctggaaac
     7621 cccgtgttta aaaatttgtt aaccgaacgt agatggtgtt catatttata tatttttgcg
     7681 cgcctaaaat ttgatgcttg gaaacaatag ttgtaaagtt cattgattat gcaaccgggc
     7741 aataaattga aaacataaac gataattaaa taaaacaaaa ataatatgta ttggacactc
     7801 gaacttgcct cgcatctgga agatgcacca tggccggcca ccaaagaaga gctgattgat
     7861 tatgccgttc gttcaggagc gcccatggaa gttatcgaaa acttgcaaga actcgaagac
     7921 gaaggcgaaa cctacgaaaa catagatgaa atttggcccg actaccctac gaaggacgac
     7981 ttctttttca atgaagagga gtattagttg ttctaaggat tgtcattcgt cattgtgagg
     8041 attgtcatta ttgtcattgc aaatccgaac agtttcgaaa gaggaaatct caactccata
     8101 tcattagatt gttattggtg aataggtatt ctgattcggt tattaataaa ttcacaacgt
     8161 agagccaaaa tcaaatcaac gaaatcaaaa cataaaaaag cctgtttccg tttttggaaa
     8221 taggcttttt ttgtaatatg aatttagtaa cacataaagc ttttgcggcc tggcgttatg
     8281 ccaatttcag gctatatatg gtggcgcgcc ttaccctcac tatgggtatc atcatgcagt
     8341 cggtaattac cggatggatg gtttatctct acaccaaaga cgcatttatg cttggactta
     8401 cgggccttgc cgaagccatt cccgctattg cggttggttt atatgccgga tacctggccg
     8461 ataaatacca tcgcaaaaaa ataattctta catccttact ttttctgatt ttaagttcgg
     8521 tttgcattac gtacatcaat tttcatgagc actattatta tacaaaattt ggagtggtgc
     8581 cactatttgc atttacattt tgtgttggca ttagtcgtgg atttttggct tcggctacaa
     8641 atgccatgtc ggccggattg gtaccgttag aagtattgcc aaacatgata tcgtggaaca
     8701 ccaccgcctg gtatataggt gctattgcgg ggccggctat tggtggcatt gtttacaata
     8761 attacaacgc tggatatgta agtttacttt acaccttact tatggttatt gcctttgtga
     8821 gttatgtttt tttaaagatt ccggccgcca ctcatacgct tacacaaagt tggtttttaa
     8881 gtttcaaaga aggagttgtt tatctaaagc aaaataaaat attattaggt gccatcactt
     8941 tagatttact ggcagtaatg tttggcgatg ccatagtgct gttgccggca tttgctgcaa
     9001 ccgttctaaa tatagatgcc gctactacag gatggctgcg tgctgcaccg gctgtgggtt
     9061 cgatagttat ggcaacatgg ctggcgtttc atcctcctgt aaacaataca gggcgcaaac
     9121 tgttgttttc ggtggcttgt tttggttttg caatgatagc ctttggcctg tcagacagtt
     9181 tcatgctcac atatatcatt cttgctttag ccggcatgtt cgatacggta agtgtaatag
     9241 tgcggaatat tattttgcaa accaccacac acgatagtat gcgcggtaga gtagcgtcaa
     9301 tcaatagtat tttcattggc tcttcgaatg agattggcgc gtttgtggcc ggtagtatgg
     9361 ctaagatgtt tacattgcag gcttcggtgg tattgggtgg ttgcattact gtgtttgtgg
     9421 tattattgat gtggcgaata gtccccgaat tgaaaaagta taatcaattg gaaaaatcac
     9481 aaaatgtatc cgaataattt taaaaaaagc tgaaacacaa aaagtgaaat ttgaaaacaa
     9541 aaacattctc aaaacagata ctaaacagaa agaaaaaaaa tacatcgatc gcttttaaat
     9601 tccgaaaaat aaattctact ttccattctg aatactaaaa gtctcaatac aaacaatctc
     9661 aatactaaaa atcccaatac tcaatacaaa caatctcaat actaaaaatc ccaatactca
     9721 atacaaacaa tctcaatact aaaaatccca atactcaata caaacagtct caatactaaa
     9781 aatcccaata ctcaatacta acgaactaaa tgaaatcggt aatagaaaaa aaaatatggg
     9841 tgattgcagg agtaatattc tgcatgattg gggtggttgg tgttatttta ccattaatgc
     9901 ccgggctggt attttttatt attgcatcgt attgtttcgc acgcgggtca cgaaaatttt
     9961 tgcgcatatt actttccaac aaaaccatag gtccacaaat aatggactat aaacgtggta
    10021 aaggcatgac catgaaaaca aaagtaatag ctattgcaat ggtacttgtt agtatgagta
    10081 taagcgcatt tgttatggta aaaatttatt gggtaaaatg ggcaatatgc gtaacttgta
    10141 ttggtgtggt ggcaataatt ttcaaacaga aaaccagaat agctgaaaca cctctcaatg
    10201 gtattgttaa aacaaattcg taataataca ttaatagatt gccgccttgt tcagtggttt
    10261 caatttcgac aacattttcc atacctttac gtttctatta atcaatagtg taatcgcttt
    10321 ttagtgcaat gaaaaacata caacataaat tattatcgtt cacaaaaatt tgtttcgtga
    10381 tacttgccgt ttctttatgt ggcagtttga aagcacaacc attgctcatc gaaaatttta
    10441 ccggaacaca atttaacaac agcatagttt taaattttac tttggttaaa ggcaacacct
    10501 gtaccgatac cgaaatacag cgcagcaccg atggcataaa ttttattgtt atcaatacca
    10561 ttgccggcat ttgtggaagc acgcaagaaa acatatcata ctccattacc gacaattcgc
    10621 ccgttgccaa cagttacaat tattatcgta tcaatttacg ttcccttggt ttttcggata
    10681 ttataaaagt gcgttatata gattttagcg ccagcaatgc gcttgttatt tacaattctc
    10741 agcaacaact ttcaacgttg ttttataaaa acccttcagc aaaaaaaatt gaattcgaaa
    10801 ttttgaatca acacggtatg aaaatataca gcggcaccag caacacatcg gaatggaatg
    10861 taagcacatc tgcctggccg cgtggtttat atcttgtaaa atttatgaat ggtgatgttg
    10921 caattacccg caagttgatg gtgttttgat tatataaaag tacacatgac aatcaatttt
    10981 gttggattat cttgtgcagg gaaaacaacg ttatgaagga aattagcagt aaaagtgaat
    11041 ttccattttt tgatttggat acggaagttg agaaatgcta acgaaaacaa attgagggaa
    11101 tacaagatga ctgtttttcg atgcaaggtt ttcaattaaa taaatggagt gatagtaata
    11161 atactataaa acattgcaac tcaaagttaa aaacaacctc aagacaaaaa gtaaaataaa
    11221 taattcgtaa atttgcaata aaactaaaca taagttaaga tggtagagtt taaaatagag
    11281 ttagacgaaa attttgtaaa aacttttgga cacaaacaaa tagaaaacta tttggaagaa
    11341 tttatgcaaa aaattgttat tcgactttcc gcaaaagaca tattgaaaga tttggaaact
    11401 gttgaccttg caaatgacaa agaatggcag gtgtcaagaa atttggcttg gcagcaagaa
    11461 aagcataaat attttgcata gagatgacag acttcgtaat tgatgcaaat gttttgatga
    11521 gtgtgcttat aagtggaaaa tcaagttaca gacccttttt gacttactac aattttattg
    11581 ttcccgaatt cgttttggta gaacttgaaa agtacaatga tgttttgaaa agcaggacta
    11641 aaatgcaaca gtttgaattt gttcaatgga cttattttgt tttttcgcaa ctcacaattt
    11701 tacctcagta cgttttgagt catgagagcc ttgaaaagtc taaaacatta cttaataaat
    11761 tagacttgaa agatatgact tatgttgctt tagcaataca gttagattta ccactcttaa
    11821 caagggataa gcctttatat gaaggcctga aaaaacaaaa attcagaaag attattttat
    11881 ttgaagattt tttaaaaaca ctttgataca aaaagagagt ccagcctata gtatttagtt
    11941 gtatctcttc aggtcctttt gaatgtcgat aaggcagacc aatttcaaat agggtttgtt
    12001 tgacttgatt tatacatcct aatacatttg agatttcaaa gtataggctt gaataatgag
    12061 atagaatata caaatctaca tcgttaaata tagctttttg gggtttatta gctatgcgta
    12121 taaacgttgg ttcgcttgag acgttttatt ccaattggag attattccgt ttgaattgat
    12181 aattttcaat ttcagtagag ggtgagtttc taatcaaaaa ttgcttttag aatccctcaa
    12241 attccaaaga ccaaaaaggc tcaatttctt ttcaaatcat ttctcaaatc aaaaaaaatg
    12301 gcgcaaagta aatgttgatt ttactattgc gccactcgtg tcaatgactg attctttaaa
    12361 aattataaat caaattttat tccttgtgcc aatggcaatt ctgtagaata attaattgta
    12421 tttgtttgtc tgcgcatata atatttccaa acatcgcttc ccgattcgcg acctccgcca
    12481 gtttcttttt cgcctccaaa tgcaccacct atttcggcac ccgatgttcc tatatttaca
    12541 ttggcaatac cacaatcact tcctgcatgc gataagaatt tttcgctctc caataagttg
    12601 cgggtaaata tggatgacga taatccctga ggcacatcgt tttgcatttg tattgcttca
    12661 tcaatggtat cgtacggcat gatgtataat attggagcaa atgtttcttc ctgcactata
    12721 tgatattcgt tttttgcttc tactattgtt ggcaacacat aacatcccga ctcgtaacct
    12781 tcgcccttac aaacatcgcc accataaatg atttttgctc cctgagcttt tgctttttct
    12841 atagcgctca aataatcgtt tactgcccct ttatctatca acggacctac aagtgtattt
    12901 gcatctaacg gatgccctat acgtatgttg ccatatgcct gcacaagttt tgttttgaaa
    12961 gtatcgtata tgcttttatg aataaataat ctgcgggttg atgtacaacg ttgaccggct
    13021 gttcctactg cgccaaataa aacggcttga aaggcaatat tcaaatcggc attttcggta
    13081 atgataatag cgttgttacc tcccaattct aaaattgatt tgcccaatcg ctctcccact
    13141 attgcaccta cacgttttcc tacgcgggta gaacccgttg ccgaaatcat gggtatgcgt
    13201 ttatcggcaa gcatgttatc gcccacatat ttcgaccctg ccgcaacaag acaaagcacc
    13261 ccttcgggta cattattttc tttcaatacc tgagctatta ttttatgcac tgcaatggca
    13321 cacaacattg ttttcgatga gggcttccat ataactgtat cgccacaaac catggccagc
    13381 atggcattcc acgcccatac cgcaaccgga aaattgaagg ccgaaattac accaacaatg
    13441 cccataggat gatattgctc gtacatacgg tgtttggggc gttcgctgtg cattgtaaat
    13501 ccgtgcaatt gacgcgataa ccctactgca aaatcgcaaa tgtctatcat ctcctgtacc
    13561 tcgcccaaac cttcctgata tattttgccc atctcatacg aaactaattt gccaagtggc
    13621 tctttgtact cgcgcagtac atttcctatt tgacgcacaa cttcaccacg tttgggtgcc
    13681 ggtatcattt gccattgctt aaatgcagat tgtgaggttt ttactatcgt atcataatct
    13741 gcagttgtgg cttgatgcag cgttgcaatc aactcaccat cactcgatga gtacgaatct
    13801 attacctcac cttttgtgtc aaaccattgt gtgccggtac atgcaccact attcattttg
    13861 tcgattccga gttttttcaa tacttcggct ataccatatt ctgcctttac ggctgcggct
    13921 attgtcattt ttatgtatta tttttttgca aaagtaattc ttataactgt tttagcgtta
    13981 gggttatgta aaaagatgag aattaaatat aattatatag attttgtaga ataatattat
    14041 atatttgcca atgaaaatcg ctcactctcc aaattttgta ttttattttc tattctaaca
    14101 atttaatttt tgaatgttgc tatgaaaaaa ataaataaat ttatgcttac gctagatgcc
    14161 ttggcaaata gcttcaataa ttggttttgc cgcaatttcg aatggttttt taccaatggt
    14221 gtaaaggcag aatacgataa aaattggtat cataaagtcg aataaagcta ttcttaccat
    14281 ttctttgctt taccctacaa cctagctgtt tttatagctg ctcaattctt ttttctcctt
    14341 tcttctattt tgtttgtttt attatatact tttcggcatt ataattcttt gttatgccat
    14401 tcaacagacg ccacttcctc aagcaaattt cggccatttc gataggttca gtttttaacg
    14461 ctcaatcttt tgccaaagac ttacagtatt tcgattcgaa atttattgat gccgacagca
    14521 acaccattgc acaagacgag gaatattggc aatgggtacg cagcaattat actatcaatc
    14581 catcgctcat caacttaaat aatgggggag ttagtccgca accaaaatcg gtgcaggatg
    14641 cctttaaacg atattgtgat ttatgtaacg aagggccttc gttttttatg tggcgcacac
    14701 tcgataccgg ccgcgaaccg ttgcgacagc ggattgccga tgttgccggt tgcctgtccg
    14761 aagaaattgc cattcatcgc aacactaccg aagctttagc aaatattgtt tttggtattg
    14821 aattacaacc gggcgatgaa gtagtgctca cacatcagga ttacccaaac atggtacatg
    14881 cttggaagca acgcgagctg cgccaaaaaa taaaacttgt atggttaaat ctcgatttgc
    14941 caatagatga cgatgatgcc ataaccaaaa catttacaca agcatttacc tcaaaaacca
    15001 aagtagtaca cattacccat gtgatgaact ggacaggcca aatattgccg gtacgtaaaa
    15061 ttgccgatga agcaaaaaaa cgcggtatac aagtagttgt ggatggggca cattcgtttg
    15121 cgcaattgga ttttcgtatc gatgaattga actgcgatta ttttggaaca agtttgcaca
    15181 agtggatggg cgctcccttc ggtaccggat ttatgtatgt gcgtaaggaa aaaatttctt
    15241 ccctgttttc gcttatgcca aacgataaac ctaacggtga cgatatacgt aagttcgaag
    15301 cattgggcac gcgtaacttt gcagccgagc aagctgtgat gacagccatc gattttcatg
    15361 aggccatagg cataaaacgt aaacaggcgc gattgctttt gctaaaaaat tactggacac
    15421 aaaaagtaaa acatctgcca cagctaaaat tcaacaccac tttgaatgat aaatattcca
    15481 atgccattac caatgtgata atggatggaa tgtctgcgca agagatggaa gctttcttat
    15541 atgacaaata taaaatacat acttcaagcg ttaactgggc taacattcat ggattgagaa
    15601 ttactccaag tgtgtataca tcgttaaacg agttagataa acttgtacac ggcttcgaac
    15661 tgatggctga aaaaataaac gtaaaaaaat aattttgttt ttaaaataaa tgagcttcaa
    15721 ataatttgag attacagaat gttagaaaaa ctgcaacaat acactaccgc atttggaaac
    15781 attgtttttc caaacggatg ctttatgtgc gaccgtaatt tgataaaagg cgaaggcgtt
    15841 atttgtactt attgcaaaat atcgttgccc aaaacgcact ttcaaattat agaagaaaat
    15901 gcagtgataa aacatttgtg gggcaaagca aacattgtgg cagcaaccgc atttttgcat
    15961 tttataaaag gctcgggtgt gcaggaggcc attcatcagt taaagtacaa aggcaaaaag
    16021 caagtgggtg tggtgctggg cgagtggttc gggtacgatt taattcaaag cgaaccttat
    16081 gcatcggccg attttctggt gccggtaccg ttgcattcag cacgttttca cagccgtggg
    16141 tacaaccaaa gcgagatgat tgcaaacggc atgtcgaagg caatgaatat tccggttgaa
    16201 aaatcgttgc tgcaacgcgg agtggaaacg caaacccaaa cacgcaaaca tcgctacgag
    16261 cgatttgaaa atatgaaaga tgtgtttttg cacaacgatg atgaaaaatt ccacaacaaa
    16321 aaatatttaa taatagatga tgtactcaca accggaagta caattgcagc atgtgccgaa
    16381 gtgctcaacc tattgccggg agccaaagtg atggtggcta cattggcgtt tgcgaataaa
    16441 tgaaattgaa attgcttttc ttgttttgaa ataaaatttt tactgagata aattttctca
    16501 acaagcaaac aatagttgtg atgaaaaaat aaaatagctt tgcatgaata caccaaaaca
    16561 tttgtgacat catataatta acaaacatgc ttcgtaaaat ttcggtattt atcctattcg
    16621 tttttgttgc acaaatggct acttcacaaa ctgtagaagt attaaataaa cccaacaaac
    16681 gaaacctaac caccggatat tatttcgatt gtttgcacgt gcaaatagac actacgaaat
    16741 tagtttttat ggcaacgctt aaagcaacgg gcgaaaaaaa cgaagggtac atatcagcac
    16801 tttatacggc cgtaaagcaa agagcaaatg agcttggggc aaattgcttt agcctacgtt
    16861 cgtatacatg tttagatgat tcggaaaaag ctgtgcttat tgttgatgcc tattacgcca
    16921 acgattccat acttacacta aacgaaaatc tacatgcaaa aaatattatt tacattttct
    16981 caaccgaaaa atggaacgat gcacttgagt acaattttaa aataaatgaa aatgtaaaat
    17041 cgttgcatgc ttcggaatat tgtgagtaca aattaagcgc tgacgaaaaa gcaacattgt
    17101 atttcggtaa aaattttata attaaggagg agtttgaata tcacgaaaac agagaggcca
    17161 agtttgtttt tgttccttat cgcggcagca aattaaaacc cgaagttttt atattttcga
    17221 atggtattta tttcggtgtg caaagcaaaa gaaacaaact ttcgcatata gaaaatagtt
    17281 tgggatggtt gcttgtttgt atatttcata aaacacaata gtgtaatttt caactagcct
    17341 tattgttttt tatttgagtt ctcttaatgt aaaaggaaaa catcagagtc ctttttccaa
    17401 aaatattccc gttagtttac aatgacatca tctttttttt aagtttgcaa ctaaattatt
    17461 tttccttgtc acaagatatc ctcataaaca atagccttac caaagctgaa aagtatgctg
    17521 agttaattcc acaactaaaa tcattggtgg atggcgaaga caatgctgtg gccaatattg
    17581 ccaacataat ggcagcatta aaatacacta tgaacttttt ttgggttggt gtgtattggg
    17641 ttgatgataa ggaattggta ttaggaccat ttcaagggcc ggttgcttgc acccgcattg
    17701 catatggtaa aggggtatgt ggcacctcat ggcaaaaaag tgaaaccatc atagtagatg
    17761 atgttgatgc attccccggt catattgcct gtagcagtgc ttcaaaatcg gaaattgttg
    17821 tccccatttt ttatcaacag aaagtaatag gggtattgga tgttgatagc gatcaacttt
    17881 caactttcga caatgacgat gcagaagcat tacaacacgt ttgcgatatt ttatcctcag
    17941 tacattcagg caacataaaa taagtaacaa tatgaataga tttattaaat tattggcagt
    18001 gaccttaacc ttcgtatatg gttatagtta tagtcaggag cttacaaaca agaagggaag
    18061 cttactaaag tttaaagttg taaaagaagt aactgccaca acagtaaaga accaacaaag
    18121 cagcgctaca tgttggtgtt tttctactaa ttcgctttta gaaagtgaat tgatgcgcat
    18181 gggaaaaggc tcaattgata ttgccgaaat gtttaccata cgcaatatgt atttactcaa
    18241 gggcaaacac tatctgcgct atatgggtaa ggcaaaattt ggggatggtg gcgaaccaca
    18301 tgatgtgatg gccgccattc gcgattatgg aatgctgcca caaagtgctt ataatgtagt
    18361 gccgggaaat aacgatcgta ttgcaaccgg tgaaatggaa agtgtgctca aaggcatgct
    18421 cgataatatg ttggcactta aagatggcaa actttcgcct gtagcattca aagcatattc
    18481 cgctgcactt gatgcctacc ttggtgctcc acctacagag tttgagtata acaacaaaaa
    18541 atatacaccg cgcacctttg ccgatgaaat gggaataaag cccgatgatt atatagagat
    18601 aacctcgttt acacatcatc cattctattc gaagttcatg ctcgaagtgc ccgacaactg
    18661 gatgaacgga atggtgtaca atgtgccgct cgatgagttg caaaaaattg ccgacaatgc
    18721 aataaataat ggattcagca tggcatggtc tagcgatgta agcgagaaag ggttttcata
    18781 taaaaatgga ttggctatag ttgccgaaac ggaatttgag aaaatgactc aggatgaaaa
    18841 agacagcatg tttataaaac cgattaccga aaaaataatt acccaacaaa tgcgtcagga
    18901 tgcttttgat aatttatcta ctcaggatga tcatggtatg cacattacag gaattgctac
    18961 cgatcaggca ggcaagcaat acttcattgt aaaaaattca tggggcaccg aaggaaatga
    19021 ttgcaatgga tatttttatt gctccaatgc atatttccaa tttaaaacaa ccgctatcat
    19081 ggttcataaa agtgctatcc ctgctgacat tgcaaagaag atgggaattc ggtagttggt
    19141 tgttagggat ggtcattgtg aggatggtca tttgtcattt caaataccgt cattggtcat
    19201 tgtgaaatag ttattggtta ttgagttatt tggttgttgc ctttggcatg ctgagtgtca
    19261 cagtgagtgt tgtcaccctg agcgcagtcg aagggattct cgaactgcga agcatctctg
    19321 cttattattg gtcattgtca tcctgaacga agacgaaggg gtcattgcga attgtcaaag
    19381 tgagtgttgt caccctgaac gaagtcgaag ggattctcga acttcgaaac aatctctgcc
    19441 agtcattggt catttgtgcg ccagccattg tttgtgtcct cacaaacaat tcccaagcac
    19501 taaattgttt caattatttt tggtcaatct cgtcattgcg aattgtcacc ctgagcgcag
    19561 tcgaagggat tctcgaactg cgaaacaatc tctcccagtc attgatccta acgtgcattc
    19621 catttttaat ctttaatttt ccatttctaa tttcattatc attggtccgt tagagcattc
    19681 aatttttaat ctttaatttt caattcttaa tttcatttcc ctactttcgc catcaagtcc
    19741 tcttaatcct tttaaaatat tattgatatg aaaaaattac tcgcaattta ttttttgcta
    19801 tttgcattta ttcaaaactt gaatgcacaa caagtacaag gcggccaatt tgaatttaaa
    19861 cataccgagt gtttgaatga acagcaacgc gaaaaaatat ttatcgactt agaacaaagt
    19921 cgtcaatcgc ttattgcaca aggtttgttg caagcggtaa atccaaatgc caaaatgctt
    19981 acacttttcg attggccgtt gaaacaagca gcaggaataa atgaccccgg ttattactcc
    20041 atttctaatt atgtagatca ggataacact gcaaatatcc tcgattacaa ttgtggtaac
    20101 ataacataca atggccacca tggtaccgat atttttacct ggcctttttg gtggtataaa
    20161 atggataaca atcaggtaga aattatttcc gctgctgcag gtacgattct ttataaaagt
    20221 aacggtaact tcgatcgcag ttgtgcttcg aataataata actggaatgc cgtgtatgtg
    20281 caacacagcg atggtagtgt ggcatggtac ggacatatga aaaacaatag cttgacaacc
    20341 aaggcaatag ggcaaacggt agtggctggc gaataccttg gtgtgttggg tagttcgggc
    20401 aactctaccg gtccacattt acatttcgaa gtttacaacc cctcaaacgt tttggttgat
    20461 ccctttagcg gtgcttgcaa ttcattaaat gcattaacat ggtggaacaa tcaattgcct
    20521 tacaagcaat cgggcttaaa taaaataatg acacacagca caccgccagt atttccagct
    20581 tgccctacaa ccgaaacacc aaatgcaaag aatgaattct gtgccggcaa tactgtttat
    20641 ttttctgcct attatcgcaa tcaacaaaac ggacaagtgc ttactcataa aatttaccgc
    20701 cccgataatt caatatttca acaatggact caaacgttta ctacatttta tcaggcttcg
    20761 tggtggtact attcgtggac acttcccggc aatgcacctt ccggcaattg gcgctacgaa
    20821 attgcatata atggaagcac ctataatcat acatttacgg tggggcctcc tgttgtagtg
    20881 gttacaccgc ccggccctgt gactttatgt gccggcagtt cagtaaccat tatttcatcg
    20941 gccggtaccg gaaatatttg gagcaatggt gctactacac aatccataac cgtaaatact
    21001 gccggtacgt atacagttag tgttagtaat gcttgcggca cagccacatc gacccccgtg
    21061 gttgtaaata ctgttgcttt gcctacagct actatatcga ctaacactcc aactacattt
    21121 tgcagtggtg gcagtgtaac tttaaatgca aatactggag ttggcctcac atatgtttgg
    21181 aaaaaaaatg gtgttgtgat tgccaatgca accgcatcga cctatgtggc aaataaaaca
    21241 ggaaattatc gtgtggtggt taccaatgcg aatggatgta gtaaaacttc tgcggctaaa
    21301 gttgttacgg taaatggttt accaactgcc actgttactc ctgccggccc aattgctttt
    21361 tgtgccggtg atagtgccgt actctctgcc aatcagggta taggtttaac gcatcaatgg
    21421 aaaaaattct cgaacaatat taatggcgct acatcattta gttataccgc aaaaactgca
    21481 agtacgtata aggtagtggt tacaaatgct aatggctgtg tacgaacttc taatgctgtg
    21541 gttgtttctg taaactgcag gctgtttgct gcaactaata ataattctat tcaatatgat
    21601 gcacaatcaa attcgataat agtgatagcg gaagttgatg aggtaacaga tggaacatta
    21661 agcatttgga gtatggatgg ccgcaaaata attgagctga gcgcaatgcc aaacgaaatg
    21721 acaaagttga aacctgaatt aaaatcagga gtatactttg ttcagtttca aaatgacgaa
    21781 caaactattt cgtcaaagat tattgttcag tagagattgt tttatgagca attaaaatat
    21841 ctcaacaaca aaatatctaa ctatcttctc ttcttcgatt ttttgcctga gtatttttgg
    21901 ttgcgtttag gatgactaaa attcgaaggt tgcctttcgg ttcttgcacg cattggtgca
    21961 tcttcatcat acaccataaa atcaatttgc ttgcgttgca ggtcggtatt ttttaccatc
    22021 acttgtaatt tatcgccaag ctgaaaagta ttaccactgc gcgaaccacg aaggcaataa
    22081 tttttctcat caagttcgta atagtcatca ttcaaatcgc gcagccttat catgccttcg
    22141 catttgctat cgaccaattc tacaaacatg ccccattcac tcactcccga tacaatgcca
    22201 tcgaacacct cggtcatgcg tgcctgcagg tattgcactt gtttgaattt aattgatgca
    22261 cgttctgcct cggttgcctg cacttccata gccgatgaat gtttgcatgc ttgcgcataa
    22321 tatgctacag gttcgctcgc tttgccatcg aggtaatatt gtaataaacg atgtgccaac
    22381 acatcggggt agcgccttat tggagaagta aaatgtgagt agtaatcgaa agccaaacca
    22441 tagtgaccaa tattattggt atcgtatttt gcttttgcca tgctacgaat tgcaagtgtt
    22501 tccaataaat tttgttcggg cttgcctgcc acatctttta ttagtttatt gatggacttc
    22561 gaaatatttt tttcgctttg cacatccatc ttgtaaccaa acttggcagc aaactgtgca
    22621 aaggttttta tcttttcagg atcgggcaca tcgtgtatac gataaacaaa agttttatgg
    22681 cctgccacat ctttggtttt tccttcggtg ttcaataaat attttgcact gtactttcct
    22741 acaaaagtgg caacgcgttt attagcaagc aacataaact cttctataag ttgattgcta
    22801 tctttcatca cttttaaaaa aacacctacc ggcatttttt cttcatcgag tttaaatttt
    22861 acttctactt tatcgaagtt aatagagcca ttaataaatc tttgtttgcg cagtgctttg
    22921 gcaattttat tcaacaataa taattctgtt gccagtgttc cttttttatc ttcgagtact
    22981 tgttgtgcct gctcatagct gaatctatta tcactatgta taacagtgcg accaaaccat
    23041 tcgttaatca attcaccggc ctctgtcatt tcaaatacag cactaaaaca aagcttgtct
    23101 tcatgtggcc tgagcgaaca aacatggttc gataattttt cgggcaacat agggattact
    23161 ctgtcaacta aataaatgga agtggcacgc gaatatgctt ccttatctaa tgcagtaccc
    23221 ggcactacat agtgcgatac atcggcaatg tgaattccta tttcgtaatt tccgtttggc
    23281 aatttttgaa ttgataatgc atcgtcaaaa tcttttgcgt cttcgggatc aatggtaaat
    23341 gttgtgatgc cacgcatatc gcgcctggct ttaatttcct ttgccgaaat atcgagcgga
    23401 attttatcag cctcatcctc cacttcttta tcgaacgata gtggaaaacc atattcaact
    23461 aaaattgcat ccatctctac actatgatta ccgggcatac ccagtactcg cacaatttcg
    23521 cctgcaggat tttttgcatc gggcagccac tctacaattt ttgccacagc cttttctccg
    23581 tctttagcgc catgcaattt acccggaaga ataaatatgt cagtattgtt tttaacgcca
    23641 cttggaatta gaaaagcaat tttattttga atatgcactg tacctacaaa ctcggtacgg
    23701 gcgcgcttca ccacttctac tacttcgcct tcggcacgcc tgttagcgcg atgtgcaaac
    23761 agcgaaattt ttactgtatc gccattcaac gcattcaaag tgttgttggg agatatataa
    23821 atatcctgtt caaagtcggc attctttaca taagcacttc cgcttgccga aaaatcgatg
    23881 gtgccttcta ccaaaacttc cacaggcaca attttatatt tgcctttatc ggtttcggca
    23941 ataatttttg cagcgcacat tttttgcagg caatcaacaa tgccttgccg taaatcttct
    24001 tcggtgtaat tgtgttcgaa tatttgtgct gcaaaatatt cgtcttcgta tttcaatgcc
    24061 tttgccagtt gcttgtagtt aaaggctttg gtaacatgca tcttaaatat ttgttgcaac
    24121 gattgcgcta aggcaacatt ctgggccggt gttagttgtt ttgtttttgg ttttaatttc
    24181 ataaattctt tattctattt ctatattcag tataaaaagt agttttgtat ttgctaatat
    24241 attggcggca aaggtaattg atgtttttta gtcaaagcat ttttgcttaa aatttattga
    24301 aatgttatcc tcgagtattt taaaaccgaa aaagaaaata atgaaagctg gaataaatag
    24361 tattaaaatt ggtgcaaaca aatttattgc aatcgcaata ttaataatat gttctcatac
    24421 agttaatgca caactaacaa ttatactcga cagtgttccg cgatactaca ctccactttt
    24481 cgactcggta tatataggag gctcatttaa taattgggca ataggaagtg ctgcttatgc
    24541 cttgctgccc aatgcaaatg gaaaaccaca aataacattg aacgataccg gcaccattca
    24601 gttcaaattt gcccatggcg gctgggcatc ggttgaagtt aatagcagcg gaggttatgt
    24661 gcccaaccgg acttatacct tcacaggaaa tgccgatacc atccatatta caattcccaa
    24721 ctgggccgat tcttatactg catcaacaca cactgtaact aatactgtat acgtagtgca
    24781 ggataatatg tggatgccgc agcttgcacg ctatcgaaga gtgtggattt atttacccaa
    24841 cgattattat acctcaaaca attattaccg cacagtatat atgatggatg gtcaaaatgt
    24901 ttttgatgca gccacctcgt ttgccggtga atggtatgtg gatgactcgc taaaagtggc
    24961 gcaacttgcc ggggactccg gtgctgtagt tgtggcaatt gacaacggtg gacagtacag
    25021 actcgatgag tattgcccat ttattaatac acaatatggc ggagggcaag gcgatttgtt
    25081 taccgacttt ttagtcaata cactcaagcc tgtgatagat gcaaatttcc gcacctatac
    25141 cgatagagag cacaccgcaa ttgccggcag cagtgttggg gcttatattg ctttgtatgc
    25201 tggcataaag catcaggcag tgtttagtaa agtgggaata ttttctcccg cctattggtt
    25261 tcaagacagc atatacactt ttgtaaaaaa tagtactgca caattcccca tgcgatttta
    25321 tcaggtttgc agcgtaaacg aaggtggcag cgtggtatat aacatgaacc gcatgcgcga
    25381 tactttaatt gccagtggat ttagttcggg cgatatttat aatatttcgc gcagctatgg
    25441 ctcgcatagc gaaacatttt ggcgcaaaga atttattccg gcatacactt ggctgtttag
    25501 taattccatt ggcaattact ttatcgaata tatgcacagc gataaattat cagtataccc
    25561 aaacccggct gatacattta tttctattgt tcatcatttg aaaaaagaag aaataaaatc
    25621 gatccgcatt tttaacgttg aagggaaaga acaatttacg gcaagtaata atttagaatc
    25681 gcaagattat aaatcaacca taagcataaa cctaaccaca ggtgtatacg tagtttggat
    25741 tttagataat aacgataagt tattcactaa gaaatttgtg gtgaggcggt gatggttttt
    25801 gaatatggat ttttatgatg gtgatgaatt gttatgattt tgaatttcaa tctgcattga
    25861 tcataaaaag cataagaatc agcgttctgt tccgaaatcc aaaatatttt atttttaaaa
    25921 aagatccagc aaatattatt tcgcccgctt accactaagt gtatacctta caaaagcgcc
    25981 aagctgatga ccatcaactg tgtaacggcc tacggtgtgg tattttgtat cgggcaatat
    26041 gtgatactca gcatcaataa ttttttgaaa cgagtatgta aaatttactc ctaccgttat
    26101 catgtttttc ttttttaatt gttttgaaat agatggctcg aagtaaagtc cttttaagag
    26161 gcgaggttcg ggttcgtagt aaaaatcgtg gtcgactatg agtaaataat taaaggtatt
    26221 tccaattcca taatcaccaa aaagcaaagt ataaaattct ggataaaaat atcgtatgct
    26281 caaaccagaa gagaacgaaa gcgaataatt atttctcatc tggtaatggt aggtggctcc
    26341 aaattttaat gcaggatcta actttgtaac atagtctgtt ctatcaatat ctctcgctat
    26401 cacatcgatg tagtcttgtt tttttaatgc cactctacca ccaagtttat ccaggttcat
    26461 gattgccgtt aaattcaacc cccatttatt atttagcata cgggtgtaac ctaacccaaa
    26521 agaggtagtc cacatttgcc aaggctccaa tttaatattg ccaataatgt ttttgtattg
    26581 gggactaaaa acccaattgt gcgatgcttc caaataaaat tgattgcgcc ttgttacgta
    26641 agtgtaaacg ggttttacgt agtttttttc tctcggtttt ttattggcat tatataaata
    26701 ttgatatgtt aagtaagcac caagttggct acctctaaaa taccaatcgc cacgactttc
    26761 gtccttttgc ccttcaaaaa atacatacga cccttcgaac atattttgca aaccaagttg
    26821 gtatttaata cctaaaccaa tttgatgatg acttttgaat tgctttaata attctgcctt
    26881 gaacaaggct gtcaaatggt aaggtcgttt ttcaagacta taggtagaga aactaaaaaa
    26941 tgggtataga ctcgaatcgc tgtctaattc gttatcataa tttgcaccga ttgataaacc
    27001 aactttatcg aaatatttaa aacccaaacc acccaaaaga ttaaggcaat aatttctctt
    27061 gaatctaaat tccatactag ggaaaaattc taaggataca tatgacttac caaaagcaga
    27121 tacaaaatta ttccagcccg tatttatttg tggataattt ttggaggaaa tattacttgc
    27181 taatccaaaa ttttgccaac cgtaaagaaa atggaactca gtgaaaaaat ctttagtgta
    27241 aaattttttt atgcctgcac caaattgagt tgtgtttagc cttatgggat taatgagatt
    27301 caaatctcct gtgccattct ttattttgaa attgctgctt accaaagatc cggcacttac
    27361 cataacagca tttttgttat atggtaattg tgcgtgcata aaattgtaaa agcaaccatt
    27421 taatagaaaa aacaaagaag gtattctcaa aagagaatac cttatatatg ttgtaaaaaa
    27481 tggttttagc ttacgcatta atatgttttt gtaaaattaa aattataatt tgtagcttca
    27541 taatagccat gagcttcgcc aatcttgtga tacctataaa acccaccatc ataagcgggg
    27601 gctttaagtc gatttcttct tttggttttt tgttctgaat aagcagtaaa gtcagcacaa
    27661 ttagtagtcc aattgccgga actttttact agcaaattag ttttaaagtt tttccacttt
    27721 ccgtttgctt ttttcttgta tgaaaccgtc tttgcataag ctttagttcc atcaccaccc
    27781 caatgagttg cagtaaattt tagcttccat ttaagttgct tgtttgagtc atatggttcc
    27841 agaccatgat gcctccaatt attcttacag tcattcaaag gaaaacctcc atgtatagtc
    27901 ttcacttgtg gagtattggc cagcacagca gggtcattac tcagtccagg tatatcgtta
    27961 catgttgcgt ttggcagtac ccacatttcg ccttgtgtat ctatcacata taagtcagtt
    28021 cctatcatta cttttcctgc atcgcttgca agtgcacttg ccacggcatc atcgtagtag
    28081 tcgataaggt cggggtcggt ggcagggttt aaagtagtgt tgtttaacca gacatcttca
    28141 agcttacgca gcttatctct ataagaaaca aagctgtggc taagttcaaa attaatcaaa
    28201 gtttggtctt cgtcaaatcc tatttggtcc gccattgcat taaaatcatc gtcattcaaa
    28261 tttgcataca gtgcttcaaa atcatcgttg tgctgttcat actcttgctc aaggcaatta
    28321 taaaccacat catattctgc ctgattagca aaaataaatg tgccgttgca taccgtgaca
    28381 ttaaatacac aatcacttcc atcaccttct ttactggcaa ttgaggcaat cgaattcgct
    28441 gtttgcggtt taatgtcctt ttccttattg caagccgaag ctaatgcaat aatagttaat
    28501 aatactaata cttttctcat agttaaataa tttgattttt ttttcctact ctttaaagga
    28561 ttttcggtta ctcctgtatt gttgctttaa gttgtgtttt gcttttgttt ttttgatgtg
    28621 tagtttttct gcatttgtct tatgataatt ttgatgctta atgttgtata tcctaataaa
    28681 tgaatttggt ttttcaaaac taactttcgg catttggctg tagccgaaaa acaattctta
    28741 atttggcaaa aaacttctct ttatggacaa aaaagcaact aaaaaactcg atataggatt
    28801 caatttaaaa cgcctccgcg aaatgaaagg ttacaaaccg gaatatattg cagagcacat
    28861 aaatgttgca ccacgcacat atctcgatta tgaaaacaac tcacatgata taggtataaa
    28921 taaattagca tctattgcag aagttttgga agttacactt tgggatataa ttacatttga
    28981 taataaaagt ttttggcaaa acaattttca caacgaaagc gaaagcaagg caacatatac
    29041 agtaaataat aaccccccat cgaataagga gcgtgaattg tatgaaaaaa taattgctaa
    29101 tttggaagca acaattgctt tgatgaaaaa taaataacac taacagttat tgtatacgtt
    29161 gtaatattat ttttagttca ttataaaatg taatgcatta ttcagcattt gtttggtaaa
    29221 gttgagcgtg ttctatttca ctgccaaaat ttcgagcgaa gatttttatg atagtgatga
    29281 tttacacaga ttttttaaaa caaagaaaat ccccgtaaca cgagctacgg ggattttaat
    29341 tttatcatat gcagataata ttatcctata attttcactt tcaactttaa gccctgacgt
    29401 aattgcttac cgctgcttat gttgttaaat tttttaatat tatcgatggt agtgccttta
    29461 ctttttgcta tgctataaag tgtatcgcct tttcttacag tataatacaa ataattaatt
    29521 tcgttattgc tttttggttc tgttttgttt ttaccttttt tggcaaccac ttttaactcg
    29581 cttttttctt gttgcgctat ttcttcttct tcggcttttg ctttgttgat tgcagaatca
    29641 tcggcagcta ttcctgctgt tgcaagtaca atttttgttg gcttactggg gtctacaatc
    29701 tccttcactt cgcgatacac taccaatgtt tgattcggat gaatggtatt gtttttaata
    29761 tgattccatt cttttaaatc gtttactgtg cagttcattt tacgggcaac tactgccaac
    29821 gattctcctt tctgtgtgcg gtaagaaagt ttttttgttt ttgtaaccaa ctgttctgca
    29881 tttgcctttg gctgagttac ggcaatttcg gtctcgatgg tctcattatt tacggcaggt
    29941 tttactgatg cagtttgttg caaagctgct gcatcatctt tcaatacttt tacccgttgg
    30001 tccacataca aggtaagatg ttgcccgcgc gaaactttat ttgaacgcaa acggttccac
    30061 gattttattt ctccctgtgt gcaattaaac ttatcggcaa cacgtgctaa actttcgcct
    30121 ctgcgaactg tatatttttg ctttaccgtt ttagttacgt aagtatattt tgcctcctct
    30181 tcctgtagtg caatattggt gcggcctttt ccaagattga tagtaggttg tgtactggca
    30241 agcaaattat cttccatttc gatagtggtt tgcttttgcg tgtaggccat tattttatta
    30301 gtaggcaaac gcaaagcata tggttggctt atagtggcag gaattaactg acgcttaaat
    30361 gtgggattca aaaatcccaa aacatcaact ggaatatcaa gattttttga aagggtacta
    30421 aacgtagttt tctttgtaac tataaccgta tccgtttcga aataagttat tgatggaggt
    30481 acaggaatta aattatgctc gcgcgaatat tccatcacat aagatatgcc cataaatgcc
    30541 ggaacgtatc cgcgggtttc ggcaggtaaa tatggcgata tagaccaaaa atcggatttg
    30601 ccacctgcac gaataatagc cttattcaca ttgcccggtc cacaattgta agatgcaatt
    30661 accaacaacc aatcgtgata tatttcgtac atctttttaa aatattggca tgcggcaact
    30721 gtagatttat atggatcgcg cctatcgtca acatacgaat ttacattcaa gtcatacaag
    30781 atgccggtat tgtacataaa ctgccaaagc cctgtagcac ctgcacaact cacagcggta
    30841 ggattcaatg ccgactctac catggcaagg tatttcaact cgagtggtaa cttttcgcgc
    30901 tccaatactt cttcaaacat tggtaaataa atggacgata atcccattat ccttttggtg
    30961 ccttcacgct tttgtgttgc atatacattg atgtatgccc gcacctgatc gttatacaca
    31021 tatggtatag gagagtttat ttttgaaatg cgatctttat aaacctgttc gggaaacgtg
    31081 ggtacattgt atatatcttc tttgcttgtt gctgtgtagt taagtgtatt gatgtttcta
    31141 aaacgtacta cattgtcaat agtaatcaaa ctatcgatag tagccgcaat agggtcggta
    31201 ggacaaacta ccagaggtgc ttgcgaaaat gcctgtgtga aagcacaaac ggaaaaggca
    31261 acggcaccga gaacaacttt taattgtgtc atttcttttt attgttactt attaatattc
    31321 aatcctttaa gagggccggc cgaaaaccgg aattgcgaaa gtatataaaa agtattgatt
    31381 tgcctaataa aaatatctac tcccctaaac tcatttaacg ttcaccttta tgaacaccct
    31441 cttaataaca tgactatttc gccctttggg ggtgtatttg tgtaatgcaa aagcaggtct
    31501 gatatttttc cacgtttcac ttcttcgtac atttttgtca actcgcggca tacgcatact
    31561 tctctatctg gaccaagtat atcgttcatt tgttgtaatg tttttatcaa tcgatgtggc
    31621 gattcgtaca aaataatggt gcgttcttct gtcagccaat cggtgatgcg ggtaacacgt
    31681 ccttttttgt gtggtaaaaa tccctcgaaa caaaatctat cgcaagggaa tcctgaattt
    31741 accaaagccg gaacaaaagc agttgggccg ggcaaagttt ctatatctat atttgctgct
    31801 atgcaagcac gtactaataa aaatcccggg tcggatatgg caggtgtgcc ggcatcgctt
    31861 accaatgcca ccatattatt ttcgcgaatg aggttcactg ttctttccaa aacgtgatgt
    31921 tcattattgg aatgaaatga ttgtatcttt ttttcaatac caagatgttt taataaaaat
    31981 ctgcttacac gcgtatcttc ggccaaaacc aaatcgcatt gttgcaatac ttttatggca
    32041 cgcaatgtta tatcatcgag gttacctatg ggcgtgggga cgaggatgag tttgctttgg
    32101 gacatgttct tagttcttcg ttacgtttat gatgagcctt gttgttcgtc ccgacacttc
    32161 ggtcgggatg ccgaccaaag tgtcggcatt ctgcgtttcg tttgcggtga tcttagttct
    32221 tagttctttg tttcgcttac gtagcggatt gttcatagtt cgtaattgcg aattgtcata
    32281 gtgagtattc tcgaacttcg aagccatctc tgcgtattac tttgttttgt gaattaattt
    32341 cagagtctct catgccattg aaaaacaaaa aataaatgag gattctgaaa tttccacttt
    32401 tgacttccct ttttcctttt aaattattcc ttttgccttt ccccttttaa cttaattcca
    32461 ccatcctaaa caaaaccggt ttgcttggtg tggcatcgtt ttctaatgct gttatttgaa
    32521 gattgagcaa atatatacca tcttcaattt tattttcgat ataaaccatt tcggtaatgg
    32581 tgcattgtgt gcgagtggca tgcggatatt gccaaaatgt atggtgtgcc tgcaacttac
    32641 catcgtcaaa ctctcggtcg accgaaggta aatcgattaa aaaatgttgc acattatttt
    32701 ttaccattat ttccatcact tcgttttgta catacgcgaa attggtgcca ctgtattgcc
    32761 gagtcaattt cgaattatca tttggtaacg ttcttaaaat cactgcatcg acatttggca
    32821 tcagcacttt tgaaatggat tcagacaaaa ttactttatc gcgattgcct gtttcggaag
    32881 gtgtaactgt aattaattgt gctacaaaaa agaaatgttt taagcattga ttaatagtgt
    32941 atggctcttt gctgatatgc cccacacatt cggtgtgagt gccgttccca tgtggattga
    33001 gcgccacatt acgaaaattt acactccctc cctgattcac atcgccaata aatgtttcgg
    33061 tgcggacggc ttcaatagtt acaggcgctg cccaccatgc atttacattg tctgccccgg
    33121 cacgcaacgg tatcgaaatg tcgatgcaat cattcagttg tgcactatat gttttgttat
    33181 taaaatttat tttggtaaac attcttaaat aattatttat acaaataaat attgaataaa
    33241 acgtatacag ttactgttat caaaaatgtg atttctgtaa atgtaaataa aatagcctaa
    33301 aatcttatgt gggcaaatat gagaaaaaaa taataccgct aaacattttt actcgtatcg
    33361 taaagttctt tgtgaaaaac taaacttatc gcatgtaaaa atatttagcg gtatcgcgaa
    33421 actaattcta atgaatcagc aagaaattat tttacaaatt ttaatgggca acggtaaacg
    33481 attttttctc tacagctttt ccatctacca tcaaggtata aaaatactga cccgcagcaa
    33541 attcacttgc attcaattgc aggcttccgg tggtgttcac gtttacggtt tttattacat
    33601 cgccattagc tgcactaatt acaattgtag ctgaagatac ctttgcatca atattaaaat
    33661 tgattgttga tgtttggttg aaaggattgg gttcgttttg tttcatccac tgtccctgtg
    33721 tattattcaa caagttgtta tcgcctttca ttgtagctat catgttgcgc atttcgtcta
    33781 attgtatttg ttgcttgttt atttgcgcct gcaacatttc gttttgtgtg ctgagctctt
    33841 gtaccgattt tactattggc accacaaact ctgaataacg caagcctaaa agttttccgc
    33901 ttttatccac accgctaaaa tcgtaattta tttttgccgc tgcagcatcc acatcttgtg
    33961 cgaggaatcc tgtgaactgt attttctcaa tatcgtattt gccttcccaa tcatcttttt
    34021 cggtaatgcc catcagttca ttttctttgc gtatatcaaa atgataagta acaggattta
    34081 ataatttaat aaatgccaaa ccgggaacat tgctttgaat attgtttttt acacgcgcat
    34141 cgctaaagtt ggtaaagcct acttgtccac caatagacga taccgaagtg ttaccaacac
    34201 gaactttatt acttgcatct acacttgcat cgtagccaaa tgccgaactg tttgtaatgg
    34261 tgctaaaact gcctgttgca taagtaccta cataactatt atttgtgcca gttgtaattg
    34321 atatgccggt aaagtaacct aacgatacat tgcgactgcc ggttgtgagc gattgaaaag
    34381 cagagtatcc cgacactgta ttataaaagc ccgtattgca tccggccata gcataaacac
    34441 ctaccgcagt attcgagaag ccactggtgt tatttctcaa cgcattcgaa ccaatagcag
    34501 tatttaagaa gccgctgttg tttgaaaaca gcgccttttg gcctaccgca gtattatcgg
    34561 aactaaggta ggcctgtgca cctcctccaa tgctattatt gaacaatgcc gaatcgccaa
    34621 tagcaaccaa tcccgatttg cccgaattga aatataacgt gtatgcaccc atagcaacat
    34681 ttgcagatcc ggtatttgcg ttatagccgg agttcattcc aaagtagcaa ttgtttactc
    34741 cggttgataa agtataggct gcactgcggc ccattacagt attattgttc gatgtgcaaa
    34801 tgccacccac actgcggcca acaaatgtgt tgttgctttg tgtagtaata cttgtgcctg
    34861 ctccttcacc cacaagtgtg ttgcccgatc cgctgatgcc aagtgtgccg gcatttccta
    34921 ccataaggtt cgtgttcaaa gtggaataca atgcacgatt acctcctatg cggtaaagcg
    34981 agtcggtatt gatacttcca acttttacat cgaacggata acgtggtgcc gagttgccta
    35041 tggccactgt accggatgca tttacaaaca ctcgtgtaat gttgtttgtt cgtattttga
    35101 aagtgacggc atccttggtc ccaataaaat tggtagaagc acttgtgccg gtgtttccgg
    35161 ttagcgacca gctttgggcg ctaccgtcat tgtgtaagca acctattgcg attgctaaaa
    35221 cgagtaaggt tttttttgtc atttttttga aatttattta ttgaggaaat taataattgt
    35281 gtcaaatata caataccaaa accaccatgt caagtgttaa tcgaaataaa tgtttttaca
    35341 ttatacccca aatgtggtat tgattattgc aaaaaaatta taacggcaat tatctaacgg
    35401 caaataaaat aaagttggtg ttacgtatac agttatagtt atgtgcttac aataattttt
    35461 cttttttgcc ttcgagcatt tctatatctt gaatggaaga accggcaatg ccttttagag
    35521 aaccataaaa attgatggaa tttgtcatga tgcgttcaaa ttgtttttcg cgctccttcc
    35581 atattttcat catcttcatt ttttcgtcca tgtaactttt ctgcaaatcg ctgaatcctt
    35641 caataatagc ttcgaattgt ccgcgaaatt cgtgccctgt gagatattca tacagcaact
    35701 ccattttact ttcttttccc gatagtgcgg tgcgtgtagt gtgcaactca tacatcatgt
    35761 atcgcagaac gagcgataat ccttttactt cccacagtgt acaaatccat acgccatctt
    35821 ttacaaaaaa ttttgtgtat ccatcgggca gtgtttcggt aacaagtaca agctgatcgg
    35881 cttttacttc gaggttgtct tcttttaatt ttacaatcca gttattatca aaatttttgg
    35941 cgcgcttact ttcgtaatat attttaccac actctacacc ctgcctcgta cgcacggttt
    36001 gaagtatatc tgcaccgcgt tgtccttttt ttatttcgtt tatatcatcg tagatatacg
    36061 tttcgcgcag aatattttct aactccaact cttgcacttc gccctgcatt tgcattgagc
    36121 cttgttcggc tttgcgtttt atggtttcta cttgttgctt gaggtcgtca attatttttt
    36181 cgcgctcacg caatttcaat acattttctt cttcggcctg ttgttttatt tttgcttttt
    36241 cttcgttcag tttatgcgaa agttcttttt ctttttcaaa tgtaatctta tcgcgcagtt
    36301 catccttctc gcgttttata atttcgtttt cggcttttag tttattgagc tgcttcacct
    36361 cttcgcgttt tgtttccagt tcgcgcatat acacctgcat ctctccggct ttttcgttgg
    36421 taatttgcac tttcaattcc tgttcaattt ttatcctctc agcagtaatt ttttcgctca
    36481 ccgattttgc aattgcattt tccatggccg ctttttcttc agccattttt agctgctgct
    36541 gttctatcat catttccttt tcgcgcaata ttttttgcgc tgcggcttcc ttggttttaa
    36601 attctttacc tatctgttct tgcagttgat gatacaatac atcgttcaca tctatcagtt
    36661 ccttacagtg cgggcagc
//
LOCUS       DAORQA010000011        36325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_242_length_36325_cov_27.659195, whole genome shotgun sequence.
ACCESSION   DAORQA010000011 DAORQA010000000
VERSION     DAORQA010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 36325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36325
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_242_length_36325_cov_27.659195"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..2741
                     /locus_tag="PK736_01955"
     CDS             255..2741
                     /locus_tag="PK736_01955"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32188.1"
                     /translation="MMKTKFYFLPVAFFCLAIILFACNSKSENNENNEANEAYDALGF
                     LSTINAFPNNDIPADAYGKAYDYYKAHFKNNNTKNATSFLPWNSMGPNNVGGRTVSIA
                     IDPVDTNVVWLGSASGGLWKSVTGGIGANAWQSVETGFPVLGVSAIAINPQNHNEMYI
                     GTGETYSYGTAVNGLLIRTTRGSHGIGILKSTDGGITWTHTLNWLYQQQRGVWKVVYN
                     YQNPSTVIAATTDGIYKTTDAGVTWLQTDTNKMVMDMVMDPVDTNIMYAGVGNLGTAN
                     GGLYRSSNGGDTWTKITNGLPANNTGRITVSVYAGNPNYVYANIANDLNSVGFYVSTD
                     KGLSWTVLNSDDISSYQGWYSKGVYIDPADNNNIFVCGVYLWQSIDGGNQFTQITDYD
                     PYDVENKPWPDMHDLISNPLDAQKLYLLTDAGLYRSDNSGGSWKSCLDGYVVAQFYLG
                     SLSASNNNIAIGGLQDRGTQRYNGTNNWDWVLGGDGTCNAIDRNNDAYQYASYQNLNV
                     QSSDDQGFTFPYTALAGNGAFVSPFELAISNQDVMYGGSNVLYQSIDKGVTWNAYGPY
                     GATKIMCIGVSPTNELKLYFASAPDDASPMQFFVSTDGGATVTDISAGLPNRYPRDVA
                     VNANNDNEAVAVFSGFGTGHVFQTIDGGQTWNNISTTLPDVPVHSVLYYSSLNTVIII
                     GTDLGVFYSNDNGLTWNHAGTGLPDAIMVFDLEYSIADQTVVAFTHGRGAYKTPFSQF
                     YPSAISEQQIFNSINIYYSAPANNLILQSESVMDGTLEIYDSNGANVYNSKIKSDRYF
                     AIDMQSWKNGLYVVQFINGKNRYVKKVVKI"
     gene            2759..3772
                     /gene="lpxD"
                     /locus_tag="PK736_01960"
     CDS             2759..3772
                     /gene="lpxD"
                     /locus_tag="PK736_01960"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRC32189.1"
                     /translation="MKFTAKQIAESLQGTVEGNADVWVNNVSKIEEGKPNTLSFLANP
                     KYEEYIYNTKASIVLVNKTFVAQKKIDTTLIRVDDAYQSFAALLEIYNNVMQNKSGIE
                     QPVYIAASSTQGQNIYLGAFAYLGENSVIGNHVKIYPHAYIGDNVTIGDNTTIHAGVK
                     IYSQCVIGSNCTIHAGVVIGADGFGFAPQGEGYKKVPQIGNVIIEDHVEIGANTAIDR
                     ATLGSTIIRKGAKLDNLIQIAHNVEIGENTVIAGHSAIAGSSKIGKNVMIGGQAGVIG
                     HLSVADGVKIAGQSGVGQSIDKENEIVQGSPAFSIGDYRKSYVMFRKLPEIEKRLSAL
                     EKK"
     gene            complement(3871..5649)
                     /locus_tag="PK736_01965"
     CDS             complement(3871..5649)
                     /locus_tag="PK736_01965"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRC32190.1"
                     /translation="MQKLTSFIRIQLWLCMIILPSILNSQSLRFVDEVPSPAVENNVY
                     KTAAFTTPPSSPVRTIGEWEELQGLMIAWKNSGSFYPTMLKQIVKSAKQECKVYILTD
                     NVSYVVNYLNNTANGGSVDTTNVVFLNNIQFNSVWCRDYGPWSVYTNDVDSLLTIDWI
                     YNRISRPLDNAVPLNFGNFIGTPVYQNTTAPWDFVNTGGNFMTDGFGTAFASELVVEE
                     NGPGNNFGVLHTPAELDTIVKAFMGITKYIKMPTLPYDAIHHIDMHMKLLDEQTLLIG
                     EYPQGVADGPQIEANLQYILSNFNSCFGTPYKVIRIPMPDDNGAYPNTTGDYFTYTNS
                     SFVNNTIIVPTYNIPEDAQALQIYRDALPGYKVVGINSTASIGALGALHCITKEIGTS
                     DPLLISHQKLEDTFDTLNGYTVNALMKHRSGISAATLFFRTDTTQPYTSVVMNYTGAN
                     DIWTANIPAQTVGAKVYYYVGATSNSGKSQVRPMPAPLGYWRFTVLGLASLAEHQNNS
                     MEVLPLFPNPSKGITCLPYKFYHSGNFKCWVTDITGNITATIYEGKISNGERKFFINT
                     ESFSAGVYFVHSLTNDAMQTQKLVVK"
     gene            complement(5654..6271)
                     /locus_tag="PK736_01970"
     CDS             complement(5654..6271)
                     /locus_tag="PK736_01970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRC32191.1"
                     /translation="MRDSFLTWILNNFYKESITRISTTENKLYLTFDDGPHATITPWV
                     LDLLKTYNAKASFFCLGKNVATNSEIYQRIKNEGHTIGNHSYSHFNGFTTGNKKYYND
                     IEQAAKEIQSKFYRPPYGSITPLQYQYLKKQYRIVMWDVITWDFDTMRTWQQCYDNTI
                     KQVQPGSVVVMHDSEKAFGRLEKLLPQLLEYYSQRNFIFDNIKTQ"
     gene            complement(6326..11281)
                     /locus_tag="PK736_01975"
     CDS             complement(6326..11281)
                     /locus_tag="PK736_01975"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRC32192.1"
                     /translation="MKKNIFFFALALIISLLNPLSVFATHIMGGNLYYQNLGVNGGSV
                     TFKVTLKMYRYCAPGSSPLPQDMNLGVYKQDQNNPNANKILFLSTTLQLVNQQFITPP
                     NANDTCSFTPNECVEEGIYEETIIVDTTGGGYQLIVERCCRNGNIVNVFNALDVGLAY
                     YAFIPPTNIINSSPTFAVAPVPYICALDTVSILNSAVDPDGDLLIYNFVWPYAGISTG
                     GNSNPNPSPVYNWPINEITYEPGYSVAQPFGVGGYTAIDTLTGLTSYLSPLPGFYVVA
                     VEIKEYRNGVLIGISRRDLQLIFITCPVNPAPILSNTTPQTTYTVQAGQQLCFNITFT
                     DPNADSLFFNTSGNIFNGALVNPPAVLPGTTGLSTVTSQFCWTPSCAQGSTNPYNFSV
                     IVNDNGCPAKTTNVVYTINVVPFVGVQQINGPDSICGNALNGVVYTAPGLNNSTYNWT
                     IAGGSIINGGNTNTITVNWNLNGPHTLTVTEISANGCPGAPYTFTVNIGAVPAAIAGL
                     DKTYCSGASAIIGSNAVPGVVYSWSPGTGLSSTTISNPVVTLTNGTQAPINNSIILTT
                     SLNGCTNADTVLVTVNPTALSNAGNNQFLCSANTLNLGTANNPNYTYAWSPALGLNST
                     SISNPLLTLVNNGNTPFNTTYAVVTNNVYGCSTSDTVTVTVNPVPTANAGGDIIFCSG
                     QSGQIGVTATSGYTYSWSPALGLNLSTASDPTVTLTNSGTVPVTNTYVVTTSWFGCAK
                     YDTVLVTVKPNPISNAGANLSICSGDTITLGTANTNGYTYAWTPAIGLSSTTVSNPTI
                     VFSNTTNNPVTYGYTVTTTLNGCITSDSVAIIVNPLPTVVPAANPTTICVGQTAALTA
                     VGANIYSWANSLNPGSPIGTGSPFNVSPLITTTYIVTGTSSANCKNTSTITVFVNPLP
                     SVAAIAVPDSICQGDSILITGSGANTYQWALLLTPGTVFDTTGAIVVSPMTTTSYIVT
                     GTDGNGCINKDTITIKVNPAATVANIIGTLSVCPGITGLTYYVPNATPSSTYQWTITG
                     GTITSGQGNDSILVDWGPAGVGIITVVETTNLGCSSDVVGISIIINPLLTPAMPYGDT
                     VLCANEAQGIIYNALNTPGSIYTWFASVGGNVVSGNGTSTITLDWTIPGLTTVYLWYE
                     ESTVADTICFGTSDTLAITILPVPQTSAIQGVQQLCIYDTSAFSVTNSVGSTYQWIAT
                     NGTVATGQGTNTTTVFWNASGNQNLAVIETNSYGCVGDTINYTPLINPLPNANAGSDV
                     AICIGKDVQLNASGGTVYNWNPSVGLSNPNISNPLSNPTSTTTYTVLVTDTNGCKKAD
                     SVLVTVNPLPIVTCFPSSPAICIATSTTLNATGGLIYQWSPNYALDDSTLSNPTANPT
                     VTTTYTVIVTDLNTCSSSASLTLVVNALPIITAGTDVLICNGASTTLTASGGITYVWS
                     PSVGLNATTGNPVAANPTTQTTYTVTGTDANGCSNTDEVIIDINPQPEASFTGEYSVS
                     CKGILGKFTNTSINALNYIWWLDDGITSTDINTEHLYNFGQAATITLIAINGICSDTI
                     SVPNDVLSLADYLKDIPNIFTPNGDGLNDCFALPKKFDFADCFTMSIYNRWGNKMFET
                     SSTEKCWNGDNLQNAACPSGTYFYVIKIKEMQISKPLQLMR"
     gene            complement(11490..12377)
                     /locus_tag="PK736_01980"
     CDS             complement(11490..12377)
                     /locus_tag="PK736_01980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC32193.1"
                     /translation="MQLITPLHFAEYELIDCGGFEKLERFGKHITCRPEPQALWDKAL
                     PQKEWDKLQHVKFVAVSSSTGKWNKTKEMPDQWFINYPFQHIDNSRKKISLRLGLTSF
                     KHVGVFPEQAVNWDFIYNSVKKLKTEKPKVLNLFAYTGASSLAACAAGADVTHLDSIK
                     QVVTWARQNMETSNLDNIRWMVEDALTFVKREVKRGNKYNGIILDPPAFGHGPNGENW
                     KLEDMMNDMLKNVKQILLDEDCFLVLNTYSLGFSSLLIENLMDSIFGKSTDREFGEIY
                     LKSTTNYNLPLGVFSRFYK"
     gene            complement(12429..12704)
                     /locus_tag="PK736_01985"
     CDS             complement(12429..12704)
                     /locus_tag="PK736_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32194.1"
                     /translation="MKKIIMLSLMVFAMSCAKKVAVSPAPVLTDEMAAAKETYEANCQ
                     RCHHLKNPAIFTALEWPRLVDKMAPKAGINEEKKAAILSYVLAFAKK"
     gene            12770..13258
                     /locus_tag="PK736_01990"
     CDS             12770..13258
                     /locus_tag="PK736_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRC32195.1"
                     /translation="MIQIRAAEINDVPSITEIYNEAVLNTTATFDTKIKTLDNRIEWF
                     NQHGAQHPVLVAIVKGEVAAWASLSRWSDRAAYDTTAEESVYVSKEFRNKGIGKQLLK
                     AIVAEGARTGKVNLIARITTDNESSIHIHHECGFTDVGVLTKVGNKFGRYLDVLMMQK
                     LF"
     gene            13607..14251
                     /locus_tag="PK736_01995"
     CDS             13607..14251
                     /locus_tag="PK736_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32196.1"
                     /translation="MDTSKEHLDQLAHIRTMMERSTRFISLSGLSGIFAGIFALIGAG
                     FAVIYLKLGFPYSEYWITPNANLTSRSAIMKFFIVDASLVLIASIVVSTLLTVKRANQ
                     NGQQIWNVAAKRLFINMAIPLLTGGLFCLIAYYRYGYIGMIAPLMLIFYGLALLNASK
                     YTLDDVRYLALAEIVLGLFAFYKIGYGLLFWTIGFGVLHIVYGAWMWIKYERNS"
     gene            14306..14593
                     /locus_tag="PK736_02000"
     CDS             14306..14593
                     /locus_tag="PK736_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRC32197.1"
                     /translation="MIDKLNKAFESRVRLGIMSTLMVNDEVDFNTLKELLDTTDGNLA
                     SHASALESLGFLQVYKQFIGKKPNTSYKVTKDGRKAFNKHLDALEALLKNR"
     gene            14691..15170
                     /locus_tag="PK736_02005"
     CDS             14691..15170
                     /locus_tag="PK736_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32198.1"
                     /translation="MSKSKLTSQIRIWIIFFVIVLVLSGVTAFPIQTEINYLANSCKW
                     LPTFLYNWILQIQQGINDSYAKYPFLAYGTDWLAFAHIVIGMAFYGVYKNPVRNIWII
                     DWAMLCCLAVFPLTFIAGYIRHIPFFHQIIDCSFGIIGLIPLKIVKNKILKLEAMQP"
     gene            15245..15919
                     /locus_tag="PK736_02010"
     CDS             15245..15919
                     /locus_tag="PK736_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32199.1"
                     /translation="MKSVFNKTLLLLVIFSIAMGFLETAVVVYLRELLYPQGFQLPLM
                     PMKNIIIITELLREAATIIMLAIVGYMAGKNSAQRFGYFIFCFAVWDICYYLFLKLLL
                     NWPASLGVWDILFLIPIPWVGPVAAPIIVSLTLICWCLYLQLHSKKITWHSWLSFATG
                     STIIIFSFTYDYWVALFKGQDLVQFSNMHIPQNYQWWVFAIGQAFLFFTIYVYNKQST
                     KLYTIK"
     gene            15929..16690
                     /locus_tag="PK736_02015"
     CDS             15929..16690
                     /locus_tag="PK736_02015"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HRC32200.1"
                     /translation="MINTLPIVAQLKAHVFNNENEQHNNKFTFDAEHKLIYNLLIIGS
                     LLCCTMFTVRMSATGHFYYAFLIWNLFLAWLPYFFAKALYNDHYKIFHAFYLVCWLAF
                     FPNAPYLITDLVHLPNKHTHWYWFDQSMLVMFAINGLMLGAISLLTVHKYINKFLKRR
                     FTWPSIFVLLFMCAYGVYLGRIERYNSWDIIATPISLAKNIWNDFRHPIQNNDVFIFT
                     FVIAILLTIIYLFIVSLKYPELILKKPICKILDEK"
     gene            16680..17045
                     /locus_tag="PK736_02020"
     CDS             16680..17045
                     /locus_tag="PK736_02020"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRC32201.1"
                     /translation="MKNNVLIKILHSFVYAFNGIVYLLKNETNGRVHFFATAVVIAAG
                     IYYRLNAMEWVAISLCIGMVLAAELLNTAIEKIVDFISPNYHHKAGTIKDLAAAGVMV
                     LAIVSIVVAAIIFWPKIVA"
     gene            17477..18997
                     /locus_tag="PK736_02025"
     CDS             17477..18997
                     /locus_tag="PK736_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32202.1"
                     /translation="MRWHVFIFFLFVVAISNAQNHNAVWCFGDSAGIDFSLPNTPTAF
                     ETGLRTRGSCVNVCNSNGLLLFYANTRAATVGKTTLVYNQNHQLMDNGINILGSGWYH
                     ELVIVPSPLDSNLYYLFCVGVTSNYGLEYSLIDMSQNNGMGKVILKNVQKLGSQQVDC
                     LAAVKHGNGRDWWVVGRSLNFSQGQSSNEFWKYLITPTGISPVMYQSIGSLNSTGLAK
                     IIFSPSGDTLLFSNAGDLLEYYNFDRCTGILSNTVTIRPQATVYSEWQWGVEFSANGR
                     FIYASTSKDTTHLFQYDLWSANPLATRQMIWESTFQPYTGGDLKRGPDGKIYFSNCWD
                     DGTFFPYPYPDTAYYLQNTYLSVINSPDSLGAACNFTPYSFYLGGKRTYWGLPNNPDY
                     DLGPLVGSPCDTLSVNLTPGPSPVGEGSLFLTYISAWEKLFINASGLRGKQLTATIYD
                     ATGKEKLARGKPPGGGLGVFGGHFTLDVDCTGWPSGLYIVHLQTEKEMLSKKFVKE"
     gene            complement(19040..19732)
                     /gene="tmk"
                     /locus_tag="PK736_02030"
     CDS             complement(19040..19732)
                     /gene="tmk"
                     /locus_tag="PK736_02030"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRC32203.1"
                     /translation="MSFPKFIAIEGLDGAGKSTQIALLIKFFEQHNCKTKFIHFPRSG
                     VGVYGELIAKFLRGEFGNVGDVHPQLVALLFARDRIDFAPQMLQWIDDGYYVLVDRYV
                     LSNIAYQCAKLKDETEKNILRDWILNYEFQLYKIPQPELSIYLDVPFTFTQTSLSTKR
                     TGNERDYLQGKEDIHESDFSFQASVKKEYDIITQSYPNIASITCYDDENKMLSVEEIH
                     TQIIERIRRIDF"
     gene            complement(19766..21241)
                     /gene="proS"
                     /locus_tag="PK736_02035"
     CDS             complement(19766..21241)
                     /gene="proS"
                     /locus_tag="PK736_02035"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRC32204.1"
                     /translation="MAKDFPKRSENYSEWYNELVYRADLAESSAVRGCMVIKPYGYAI
                     WEKMQQVLDKMFKDTGHSNAYFPLFIPKSFLSKEAAHIEGFAKECAVVTHYRLKNAPD
                     GSGIIVDPEAKLEEELIVRPTSETIIWNTYRNWIMSYRDLPLLINQWANVVRWEMRTR
                     LFLRTTEFLWQEGHTAHATVDEAIAETKLMLDVYANFVETYMAVPVIKGVKTANERFA
                     GALDTYCIEALMQDGKALQAGTSHFLGQNFAKAFDVKFTSKEGKQEFVWATSWGVSTR
                     LMGALVMAHSDDDGLILPPMLAPIQVVIVPIYKSEEQLQQVDAFVDPIIKSLKQKNIS
                     VKYDNRDTYKPGWKFSEYEFKGVPIRIAVGPRDMENNQVEIARRDTKEKATYSIVDLD
                     VKVENLLIQIQDNIYKKASDHRESATHYVDTWDDFVAVLDNSPGFVYAHWDGTSETED
                     AIKEKTKATIRCIPLNNKMEEGKCILTGKPSSQRVIFARAY"
     gene            21467..22987
                     /locus_tag="PK736_02040"
     CDS             21467..22987
                     /locus_tag="PK736_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32205.1"
                     /translation="MKQSMLILFTSAALFAGCNSSQQTAGGNSYDDIYAPSSVPVAEN
                     NSQAPQQYNQAAPPTNYSNDNSQPQNSGSDNRFDYGNKNGNDNNRYSDQNAQGGGNVT
                     NNYYNDDDYYDYAYTSRMRRFHRPFGWSYYDSYYTNSFWYDNNPFNYGQSIYLGYNWW
                     APSYFYTNPWFGYSGFNCWNSWGNGGWGMNYGWGFGCNNFYNPYFGGLGYQNGYWNGY
                     NQGYWDGYYNGLYGNQMNPYYYNSFDNVVNDNNYYGWHGRENNTIKNNLVGTTVSSSP
                     RPIGAIYAEAVNPRSNDGNVQSNPAPRPGKSVVDVPAPRDVKSNEYQSTPRPMQTTTP
                     GRGNNVDNYTVEPRPAKGNTQGETVPNNGNNPRGNNNTVEPHPKGTGTVDPRNNNSSP
                     RPQYENAPANNNTPRPRYDNNNAPANNNEAPRPRYDNNNAPANNNVTPRPRNNDTPRT
                     EPAPRPSKQDKPRVQPRQQSQPAPRQQQATPAPRQQQSAPAPRQNNNGGSRPRGRN"
     gene            23131..24612
                     /locus_tag="PK736_02045"
     CDS             23131..24612
                     /locus_tag="PK736_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32206.1"
                     /translation="MKQIIIGVATFAMLTASVYAQSLQSAIDFNQSLPGSTARSLGMG
                     GAFGALGGDPSCLMVNPAGLAIFRKSEFSFTPTFNYRGNSSTYLLNNVTDNRFSFNMG
                     NAAAIFSGYRENRKDWKGLNFGIAYNRDYSFSTNNYFEGFNSKNSILNSYLDKANGTS
                     DSLFSDLNFAFDVGLAYNAFLLNPDTGLTNMYTIAAPNGGVQQSMTQELRGGKGAWSF
                     AFSNNYQDKIMLGGSLDISSFRYDETNTYTESDKYDSIVSPYDKMDFKSFTLNQDKQT
                     RGSGVSAKFGIIVKPVEWLRVGAAFHTPVVYNMKYNDQYSIASSFASKQYAPDAIEST
                     FDYKYTSPLKAVGSLALVWKQVGLLSVDYEYVDHQATRFKSKGYNFVNENSAISKQLQ
                     GASNVRVGAEYRYQIFAVRAGAAMYGSPVRSGNLKGAADYAAVTYTGGVGMRESGYYF
                     DIGYGYTKNSKTTAPYTVAEQTTNDVVTDYKLHRIQFTLGFKF"
     gene            24804..25382
                     /locus_tag="PK736_02050"
     CDS             24804..25382
                     /locus_tag="PK736_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRC32207.1"
                     /translation="MFEQINANVTKCTTFSQEELNIFDALLVSKTYPKKTFLLQEGEV
                     CNFEAYVVKGCARTFYIDDNGFEVILQFAIEDWWISDIASFNDQTPSRLFIETMEDCQ
                     LLLLAPQTKEDLLFRVPKFERVFRMMIQRNLSATQNRLIGNIAQPAQERYLAFLKKYP
                     TISQRIPQHYIASYLGISPEFLSKIRAKIARK"
     gene            25611..26507
                     /locus_tag="PK736_02055"
     CDS             25611..26507
                     /locus_tag="PK736_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRC32208.1"
                     /translation="MDRKDFLKKGIMGSSIFVASAAMGQVLKNNIDELAELKTMPMEK
                     DFVGFNHIPNTNSKIMANTVLHKADTRGNANHGWLNSHHTFSFANYYNPERMHFGVLR
                     VLNDDRVEAGMGFGTHPHDNMEIISIPLEGDLEHKDSMGNVAVIRNGDIQVMSAGSGI
                     THSEYNKNKDSEVKFLQIWIFPNKKNVTPRYDQISLKAQDRHNKLQQIISPNANDEGA
                     WMHQDAWFQLGTFDKNFETQYKLKKQGNGVYAFVLKGDVTIEGTELNERDGLGIWNTE
                     NISIKSNTTGAEILLMDIPMNI"
     gene            26528..27088
                     /locus_tag="PK736_02060"
     CDS             26528..27088
                     /locus_tag="PK736_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRC32209.1"
                     /translation="MSNTNWALDPSHSHVGFKVKHLMFTNVNGTIGKYNVTVESGSDD
                     FNDAQFAFTAEVNSISTGEAARDGHLMGADFFNAEKFPQINFKSTSFKKVSEGNYELV
                     GNLTMLDVTKPVTLAVEYAGMMKDPWGNIKTAFAISGKINRKNWGLTYNAALETGGVL
                     IGEEVSINAEIQMVKKAVEATTEVVA"
     gene            27920..29392
                     /locus_tag="PK736_02065"
     CDS             27920..29392
                     /locus_tag="PK736_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32210.1"
                     /translation="MLYNETNVGQGDNGTSPCNIAGEDVVYEILTPNGADILFVTVDN
                     ASNPFLVYLEEQNCGSGICQAFTLGAGKTNLKISVNSYQKYFLWIDSPFKITYNISVG
                     ADTGSVYVNIPVTKGNLQLDASMCATPPFAANKPFFQVAYNNIFQTNPMTLSPLFIQG
                     NMCVSVFFKNTTGIEGVKKFTFYFDPNGYANVTAPALITGFYNAGNWVGTKSNNVWTY
                     IFDDSLGTGSGDFTGTPDSCLAYSFCFALTPLSNNPVLTNVIVKIEGDGKGSGFSGFV
                     HSGCCPVGFSGCNFSGGGGGGGNASAFAFGFADPGGSLPLKIVSFEVKTNQNNAQLKW
                     MVMNDGANIFYDVEKSEDGLQWSTISTVKSKPSSKEVTVYESIDKNVQQGNNFYRIKY
                     EDDNGEIIFSSVEKIFVSTMHSISAFPNPAKGLINVSGFEGQIINVKIYNLEAKEMLC
                     KYTFTNSGLFIDSSNLPTGSYCFIFTNQYETERKIIEVIN"
     gene            complement(29457..30041)
                     /locus_tag="PK736_02070"
     CDS             complement(29457..30041)
                     /locus_tag="PK736_02070"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRC32211.1"
                     /translation="MRKLLLSTTLLLVMNFTIQAQLNWIVDTHNSQLKFTVVHLMISQ
                     IDGMFTSYTGTVKSKDITFADAEINFNVETKSVDTRDADRDIELRSKEFFDCNKFPNM
                     SFKSTSFKKIAGKKYKLTGDLTIKDVTKQVTFDVTFNGTATDGFGNKKAGFKATTTIN
                     RLAYGLMWNNTLEGGGLTISNEVDLILNIQLMMK"
     gene            complement(30102..31349)
                     /locus_tag="PK736_02075"
     CDS             complement(30102..31349)
                     /locus_tag="PK736_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRC32212.1"
                     /translation="MSYNEPPFSITTLKNGIRLIHQNTGKSEIVHCGILVNAGTRHES
                     HGKEGLAHFIEHVLFKGTSHRTSMQILNRLEVVGGELNAYTSKEETCIHASVQVQYLE
                     RAFDLIADIMFNSIFPEKEIAKEKEVVLDEIRSYLDNPSEQIFEDFESKLFRNNTLGN
                     PILGTTQSVKAFTRNDIINFIKTNYHPEKMVVSVTGDISFTKAKELADKYFVSRAKAN
                     NVAAVQKFKHNKTFAQTMERPTEQTHFVMGNVAYSFRDKRRLQLSLLNNILGGPAMNS
                     ILSLSVREKYGYTYNIESNYLSYSDTGVFNIYFATEPKLFDKTYAVVNKELEKLRNNK
                     FTTTKLSQHKKQLIGQIALAQENKAGIMMALGRSLLNHNRVDTLETIFAKINAITPED
                     ILHVANEVFDKNKFSTLQYVSPN"
     gene            complement(31380..33809)
                     /locus_tag="PK736_02080"
     CDS             complement(31380..33809)
                     /locus_tag="PK736_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC32213.1"
                     /translation="MFIQFFSVAQIIGEGLSSGIEGLVVDANTNKPVPFSTAALMQPG
                     NTLPLATMLVSDNGTFAFKASANTFYVRVSFLGYTTFVSDTFNLDVTNKTRKLKIKLS
                     PTATQLNEVEVSGQKNYAQNNLDRKVYTIDKDLVAVGGSASDALKQIPAINLDQDNNV
                     NMRGSDNLQILVDGRPSGMLGGDNQSGLEQLPASSIERIEVISNPSSQFDAAGTGGII
                     NIVLKKNVQKGFNGNASVNVSTRNKYGANVGINAMYKKLNVFASYAIRDARNLMIGTL
                     DRTATFVDTAFTLEQSSRNITKQTTHTFRTGGDYQLSKSASVTTSINFIRNDGSRDEK
                     IYYDNFLLQPNYPQQFVSQQLRTNAGNDWRWSYDIAAGFRKSFAAGKVEWNTDATYSR
                     NARSDNQDATIKFFDGFRDFVESQSMLQNFKVGNGLQSLNTQSDVTWRATKKIKIDGG
                     AKYFGRTSNNSLNQYESEFTNNNHILSASSTQFSFTENITAAYVMGLYSIGKVSVQAG
                     LRNENTFNQFKYKDSESTSRSFNHFFPSVFFRYNSSATNEIGASYGKRIQRPNAEQQN
                     PFTDFSDPQNIRVGNPNLVPELIHSFELSNKRIFSFTTLNTTLYYRYNQNHIQRFRKV
                     TSDGFSVMTFINSAWRKDYGVEISATGDITKWWNVLLNVNFFNQEINAGNIEQNLGRK
                     GFSWSTRMNNNFKLQKTLTAQLSGSYNSIMRGAQGQLQPVYSADVAIKKDWMKSKYSL
                     TIGLADIFDTRIFQVRNTSTYFDFEFERKRDTRILTFTFNYRFGRFDDKQKQQRNNDK
                     QNERPGGEMDF"
     gene            34622..>36325
                     /locus_tag="PK736_02085"
     CDS             34622..>36325
                     /locus_tag="PK736_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32214.1"
                     /translation="MPKRLSLATRLFTALTVFCLLGFFAQAQFVSIPVTGFNNDIVAS
                     GVGPGTALSMPGTSNPTIGVDGAGFTFIDNTYKWQAANAFPTCFMPASGSAPSLVTTG
                     LTYNFQSYTALNALTLDNNSTTYLTSPFPSIGQLTLTTPASYTRLYVLYESVVNIGPS
                     YFINATVKFTDSTSQTFSNNTAVNWFSGTPAVTNVARAQNVSPGLGTTCPIAPNLFEL
                     PLTLSAANYGKQVAYITFTIPTSLTSGTTPDRVNYFHAMAVGGLLACSAPAAQPTLPS
                     LTTISTSQINGSFTAATGPPSGYLVVRYPNLAAETAPVNGTTYVAGQSLGLGTVVQSS
                     ALTTFNNTGLFGGTTYDYYVYSFSTATAPCGGPIYLTAGGSIGSATTTACSGVAGGTY
                     SVGPTGTYLTLTAALAAVNAGITGPVILELQSTYTSGGETFPITFPSNSCLGPINTLT
                     IRPELGAAALAITSANVTGTINFNGAKYVTIDGRPGGVGTSQLTIANTNAGASYAANF
                     VGDATNNTIMYCTVTSRNNSATSGTIAFSTGIVTGNDNNTIDNCIISDDANGTSFPVN
                     GI"
BASE COUNT        11001 a         6879 c         7160 g        11285 t
ORIGIN      
        1 ttttacataa tgtttgaaac gaaacatcgc atatttgtaa taataaaaat tatagttcaa
       61 tatttttttc ttgagcgctg cggtttttgt aaaatgaaaa caataataat ttttatatgc
      121 cgaaaataaa ccctcaatat ttgattcaaa ccatttggta tggaatgcgt catttttttt
      181 attctaaaac gtattgaatc aattgttacg gatagaaaaa ttatatttac agtcaaatat
      241 tttacattac agaaatgatg aaaacaaaat tttattttct gcctgttgcg tttttttgtt
      301 tggcaataat tctttttgcc tgcaattcga aaagtgaaaa caacgaaaac aacgaagcta
      361 atgaagccta cgatgcattg ggttttcttt caaccataaa tgcattcccc aataatgata
      421 taccagccga tgcatatggt aaggcatatg attattacaa agcacatttt aaaaacaaca
      481 atacaaaaaa cgccacatca tttttgccat ggaattcaat gggccccaat aatgttggag
      541 ggcgcacagt ttcaatcgca attgatccgg tcgataccaa tgttgtttgg cttggctcag
      601 cttcgggcgg attgtggaaa tctgttactg gtggtattgg cgctaatgca tggcaaagtg
      661 tcgaaaccgg gtttcctgtt ttaggtgtaa gtgccattgc tatcaaccca caaaaccata
      721 acgaaatgta tattggtact ggtgaaactt acagctatgg cactgcagta aatggactac
      781 taatcagaac aacgcgtggc agtcatggca ttggaatatt gaagtcgacc gatggtggca
      841 ttacatggac tcataccctc aattggcttt accaacaaca gcgtggagtg tggaaagtgg
      901 tgtacaatta tcagaatcct tctacagtta ttgcagctac caccgatggt atttacaaaa
      961 ctacagatgc cggtgttact tggttacaaa ccgatacaaa taaaatggtg atggacatgg
     1021 tgatggatcc cgttgacacc aatatcatgt atgccggtgt tggaaatttg ggaactgcaa
     1081 atggcggttt gtaccgttca tcaaatggtg gcgatacatg gacaaagatt accaacggac
     1141 taccggcaaa taataccggc cgcataactg tgagtgtata cgctggtaat ccaaattatg
     1201 tttatgcaaa cattgccaac gatttaaatt ctgtaggttt ttatgtgtct accgataagg
     1261 gcttatcatg gacggtgctg aactccgatg atatatcatc ctatcagggt tggtatagca
     1321 aaggtgttta tatagaccct gctgataaca ataatatttt tgtttgcgga gtttacttat
     1381 ggcaatctat cgatggcggg aatcagttta cacaaataac agattacgat ccttatgatg
     1441 ttgaaaataa accctggccc gatatgcacg atttaatttc gaacccactc gatgcacaaa
     1501 aactttattt actcaccgat gccggtttat accgttccga taattcgggt ggttcatgga
     1561 aatcgtgtct cgatggttat gttgtggcac aattttacct tggcagtttg tcggcttcaa
     1621 acaataatat tgccataggc ggcttgcaag accgcggcac acaacggtac aacgggacca
     1681 ataattggga ttgggttttg ggtggcgatg gtacctgcaa tgcgatagat aggaataatg
     1741 atgcctatca atatgcatcg taccaaaact tgaatgtgca gtcttctgat gatcaagggt
     1801 ttacttttcc ttatactgca ttagcaggca atggagcatt tgtgtcgcca ttcgagttgg
     1861 ctatttccaa tcaagatgta atgtatggag gttcgaatgt gttgtaccag agtatagata
     1921 aaggcgtaac gtggaatgct tatggccctt atggcgctac aaaaattatg tgtattggtg
     1981 tttcgccaac caatgagttg aaattatatt tcgcttcagc acctgacgat gcatcgccaa
     2041 tgcaattttt tgtaagtacc gatggtggtg caaccgttac cgatatttcg gcagggttgc
     2101 caaacagata tccgcgcgat gttgctgtaa atgcaaataa tgataatgaa gccgtagcag
     2161 tgttttcagg atttggtacc ggccatgtat ttcaaactat agatggcggg caaacatgga
     2221 acaatatcag tacaacatta cccgatgtac ctgtgcacag tgttttgtat tattcaagtt
     2281 taaatactgt aataataata ggaaccgatt taggtgtttt ttattccaac gataatggtc
     2341 ttacatggaa tcatgccggc actggtttac ctgatgcgat aatggttttc gatttagaat
     2401 attccattgc cgatcaaact gttgtagcat ttacacatgg ccgtggcgct tacaaaacac
     2461 ccttttctca attctatcct tcggctataa gtgagcaaca aatttttaat tctattaata
     2521 tttattattc agcacccgca aataatttaa tattgcaaag cgaaagtgta atggatggca
     2581 cacttgaaat ttacgatagc aatggtgcca atgtttataa ttcgaaaata aaaagcgata
     2641 gatattttgc catcgatatg caatcatgga aaaatggttt gtatgttgtg caattcatca
     2701 acggcaaaaa tcgttatgtg aaaaaggttg taaaaattta aaagctcaaa aaccaaaaat
     2761 gaaatttacc gcaaagcaaa tagcagaatc tctgcaagga acagttgaag gcaatgccga
     2821 tgtttgggtt aacaatgttt cgaaaattga agaaggaaaa cccaacactt taagttttct
     2881 tgccaatcca aaatacgaag aatacatcta caataccaaa gcaagtattg tactggtaaa
     2941 taaaactttt gtagctcaaa agaaaatcga caccacactt atccgtgttg acgatgcata
     3001 ccaaagcttt gccgcactgc tcgaaattta taataatgtg atgcagaata aatcgggaat
     3061 agaacaacct gtttatatcg ctgcatcttc cacacaaggt caaaatattt atttaggagc
     3121 atttgcctac ttaggcgaaa acagtgtgat aggtaatcat gtaaaaattt atcctcatgc
     3181 atatattggc gataatgtta caataggcga taatacaacg atacatgcag gagtaaaaat
     3241 ttatagccaa tgtgtaattg gaagtaattg tactattcat gcaggggtag ttataggggc
     3301 cgatggtttt ggttttgcac cacaaggcga gggctataaa aaagtgccgc aaataggaaa
     3361 tgtaattatt gaagaccatg ttgaaatagg agccaataca gccattgatc gtgcaacact
     3421 cggcagtacc atcattcgta aaggcgcaaa gctcgataac ttaatacaaa tagcccacaa
     3481 tgtggagata ggcgaaaaca ctgtaatagc tgggcattcg gcaatagcag gctccagcaa
     3541 aataggaaag aatgtaatga taggcgggca agccggagta ataggtcacc tctctgttgc
     3601 cgatggagta aagattgccg gacaatcggg tgtaggccaa tccatagata aagaaaacga
     3661 aattgtgcag ggctcgcctg cattttcaat tggcgattat cgcaagtcgt acgttatgtt
     3721 tcgcaaactt ccggagatag aaaaacgatt gagtgctttg gagaagaagt agtttttgag
     3781 accgtttttt aagcacgatt tccaattatt ttttttgtaa tacctcaatt aacaaaaaag
     3841 gcgttgcacc attccacata atactgttcc ctacttcacc accaactttt gtgtttgcat
     3901 agcatcattg gttaacgagt gcacaaaata aactccggcc gaaaaagatt cggtattgat
     3961 aaaaaatttg cgctcaccat tggatatttt cccttcgtat atggttgccg ttatattccc
     4021 tgtaatatct gtaacccaac atttgaaatt gccactatgg taaaatttat agggcaaaca
     4081 tgtaatgcct ttcgaaggat ttggaaacaa tggcaatact tccatgctgt tgttttgatg
     4141 ttcggcaagc gatgccaatc ctaaaacggt aaacctccaa taccctagcg gtgcgggcat
     4201 tggccgcact tgcgatttgc ccgagttgga agtagcaccc acatagtaat aaacttttgc
     4261 acctacagtt tgagctggaa tatttgccgt ccaaatatca ttggctccgg tgtagttcat
     4321 tacaacagac gtatagggtt gggtggtatc ggtgcggaaa aataatgttg ctgcagaaat
     4381 tccgctgcgg tgtttcatca gtgcattaac agtatatcca ttcaatgtat cgaatgtatc
     4441 ttccagtttt tgatgcgata tcaaaagcgg atcactggtg ccgatttctt tggtgataca
     4501 atgtaaagct cccaaagcgc caatacttgc tgtagaattt attcccacca ccttgtaacc
     4561 cggcaaagca tcgcggtata tttgtaaggc ttgtgcatct tcgggaatgt tatacgttgg
     4621 aactattata gtgttgttta caaaagatga attggtgtac gtaaaataat cacctgtagt
     4681 gtttgggtat gcgccattat catcgggcat aggtatacga ataactttgt aaggtgtgcc
     4741 aaagcacgaa ttaaaattac ttaatatata ctgtaggtta gcttctattt gagggccatc
     4801 ggcaacaccc tgaggatatt cgccaatgag taacgtttgc tcatcgagaa gcttcatgtg
     4861 catatcaata tgatgaatgg catcgtaagg cagtgttggc attttaatat atttcgtaat
     4921 gcccataaat gctttaacaa tagtatctaa ctcagccgga gtatgcagca ccccaaaatt
     4981 attgcccggc ccgttttctt ccactaccag ttccgaagcg aatgcagttc caaatccatc
     5041 ggtcataaaa ttaccaccgg tgttcacaaa atcccaagga gctgtagtat tttgatagac
     5101 tggtgtacca ataaaattac caaaatttaa tggcacagca ttgtcgagcg ggcgcgatat
     5161 gcgattgtaa atccagtcga tggtgagcaa tgaatcaaca tcgtttgtat atacactcca
     5221 tgggccataa tcgcgacacc aaacgctgtt gaactgaata ttatttaaga acaccacatt
     5281 ggttgtgtct acacttccgc catttgcagt gttgttcaaa tagtttacaa cataacttac
     5341 attatcggta aggatgtaaa ccttgcactc ttgtttggcg ctttttacaa tctgctttaa
     5401 catagtaggg taaaagctac ccgaattttt ccatgcaatc ataagaccct gcaactcttc
     5461 ccactcacct atagtgcgca caggcgatga tggtggagtg gtgaatgccg ctgttttata
     5521 cacattattt tctactgctg gtgatggtac ttcatcaaca aatcgtaacg actgtgaatt
     5581 taaaatcgat ggtaaaatta tcatacacaa ccataactgt atacgaataa aagaagtaag
     5641 tttttgcatt tttctattga gtttttatgt tgtcaaatat aaaatttctt tgagaataat
     5701 actccaataa ttgtggcaat aatttttcga gacgaccaaa tgccttttcg ctatcatgca
     5761 tcactaccac cgacccaggc tgtacctgct ttattgtgtt atcataacat tgctgccatg
     5821 tgcgcatagt gtcaaaatcc catgtaataa catcccacat tactatccgg tattgttttt
     5881 tcaaatattg gtattgcagt ggagtaatgc tgccatatgg cggcctgtaa aatttcgatt
     5941 gaatttcttt tgctgcctgc tcaatatcat tataatattt tttgttgcct gtggtaaatc
     6001 cattgaagtg actgtaggaa tgattgccaa tagtgtggcc ttcatttttt atgcgctgat
     6061 aaatttcgga atttgtagct acattttttc ccaaacagaa aaaggaagct tttgcattat
     6121 atgttttaag taagtcgaga acccaaggcg ttattgttgc atgcgggcca tcgtcaaatg
     6181 ttaagtacag tttgttttca gtggtgctaa tacgcgttat cgattccttg tagaaattat
     6241 tgagaatcca tgttaaaaag gaatcgcgca tttgcatcaa tataatttta catgtgtgtt
     6301 caatcgaaat gaattttcaa atgagctatc gcatcaattg caatggtttg ctgatttgca
     6361 tttcttttat ttttataaca tagaaatatg ttcccgatgg gcatgcagcg ttttgtaaat
     6421 tatcgccatt ccaacatttt tcggtcgagc ttgtttcgaa cattttattt ccccaccggt
     6481 tataaatgct catggtgaag cagtcggcaa agtcaaattt cttgggcagt gcaaagcaat
     6541 cgttaagtcc atcgccattt ggagtaaata tatttggaat gtctttgaga taatcggcca
     6601 aagacaaaac atcgttcggc acactgatgg tgtcgctgca aattccgtta atagctatga
     6661 gtgtaatagt agctgcttgt ccaaaattat aaagatgctc ggtattgata tccgtagagg
     6721 taattccatc gtcaagccac caaatataat taagtgcatt gatgcttgtg tttgtaaact
     6781 tacccagtat gcctttgcac gatacactgt attcgccagt aaaggatgcc tcgggctgtg
     6841 ggtttatatc gataattact tcatcggtat tgctgcagcc gttagcatcg gtaccggtta
     6901 cggtatatgt tgtttgtgtg gttgggtttg ctgcaaccgg attgcctgta gtagcattaa
     6961 gccccactga tggcgaccat acatatgtta ttcctccgct tgctgtgagg gttgtgctgg
     7021 caccattaca tatcagcaca tcagttccgg ctgtaattat tggcaaggca ttcaccacca
     7081 atgttaacga tgccgaacta ctgcaagtgt ttaaatcggt aactataaca gtatacgtag
     7141 tagtaacagt tgggtttgca gttgggttac ttagggtact atcgtccagc gcataatttg
     7201 gcgaccattg ataaattaat ccgcctgtgg catttaaagt tgtacttgtg gcaatgcaaa
     7261 tagcagggct tgaaggaaaa caagtaacta tcggcagcgg gttaacagta accaatactg
     7321 aatcggcctt tttacatcca ttggtatctg taaccaatac tgtatacgtt gtggtggaag
     7381 ttggattcga taaaggattc gaaatatttg gatttgataa tcctacagaa ggattccagt
     7441 tgtaaacagt gccaccactg gcattcaact gcacatcttt acctatacaa atagcaacat
     7501 cgctgcctgc attggcattt ggtaaagggt taataagagg agtatagttt atcgtatcgc
     7561 ctacacaacc atagctgttt gtttctatca cagcaagatt ttgattgcct gatgcattcc
     7621 aaaatacggt ggttgtattt gttccctgcc cggtggctac agttccatta gtagctatcc
     7681 attgataagt agaaccaaca ctattggtta ccgaaaatgc tgaagtatca taaatacaaa
     7741 gttgctgcac gccctgtatt gcactggttt gtggcaccgg caaaatggta atagccagcg
     7801 tatccgatgt gccaaagcaa atagtatcag caacggtcga ttcctcatac cataaataaa
     7861 cagtggtaag ccccggtatt gtccaatcga gcgtaatggt acttgtgcca tttccactta
     7921 ccacattacc gccaacagat gcaaaccatg tataaataga acctggcgtg ttgagggcat
     7981 tataaataat tccttgtgct tcattggcac acaatactgt atcgccatat ggcatagcgg
     8041 gagttaaaag tgggttgata ataattgata ttccaacaac atccgatgag caaccaaggt
     8101 ttgtagtttc aacaacggtt attatgccta cacctgccgg tccccaatcc accaaaatag
     8161 aatcgttgcc ttgcccgctc gtaattgttc ctccagttat tgtccattga tatgtagatg
     8221 acggtgtagc atttggaaca tagtaagtaa ggcctgtaat gcctggacaa accgacagtg
     8281 ttccaataat gttggcgact gttgcagcag gatttacctt tatggtgatt gtgtctttat
     8341 tgatgcagcc attaccatcg gtacctgtaa ctatatacga agtggtggtc attgggctta
     8401 ccacaattgc ccctgtagtg tcgaaaactg tgccgggagt taatagcagt gcccattgat
     8461 atgtattagc gccactgcct gtaataagaa tactatcacc ctgacaaatg ctgtcgggca
     8521 cagcaatggc tgcaaccgat ggtaaaggat ttacaaacac tgttattgta gaggtgttct
     8581 tgcaatttgc actgctggta ccggtaacta tatacgtagt ggttatcaat ggcgaaacat
     8641 tgaatgggct tccagtacca ataggcgaac cggggttgag cgaattggcc catgaatata
     8701 tatttgcgcc tactgctgta agtgctgctg tttgcccaac acaaatggtt gttggatttg
     8761 ctgcaggaac aactgttggt aatggattta caattatcgc tacactgtcg cttgttatac
     8821 aaccattgag tgtagttgta actgtgtaac catatgtaac gggattgtta gttgtattgg
     8881 aaaaaactat cgttggattt gaaaccgtag tagaactcaa ccctattgca ggtgtccatg
     8941 catacgtata tccatttgtg tttgctgtac caagtgtaat ggtgtcacca ctgcatatac
     9001 ttaaattggc acctgcattc gaaatgggat ttggttttac tgttactaaa actgtatcat
     9061 acttagcaca accaaaccaa gaggtggtta ctacataagt attggtgaca ggaacagtac
     9121 cgctgtttgt aagtgttaca gttgggtcgc tggcagttga taaatttaaa cccaaagcgg
     9181 gcgaccatga gtaagtatat cctgatgtag cagtaacacc aatctgcccc gactggccgg
     9241 aacaaaaaat aatatcgcca ccggcatttg ctgttggtac agggtttaca gtaaccgtta
     9301 cagtgtcgga tgttgagcaa ccataaacat tatttgtaac caccgcatag gttgtgttga
     9361 acggtgtatt gccattattt acaagtgtaa gtagagggtt cgaaatactt gtactgttta
     9421 gccccaaagc cggagaccat gcatatgtat aatttggatt attggcagtg ccaaggttaa
     9481 gcgtgtttgc actacacaaa aattgattat tgcctgcatt ggaaagtgct gtgggattta
     9541 ctgtaaccaa aaccgtatcg gcattggtac atccatttaa cgaagtggta agtatgatag
     9601 aattatttat tggcgcttgt gttccgtttg ttaatgtaac cacaggatta gaaatggttg
     9661 tactgcttaa ccctgtaccg ggcgaccatg aatagacaac acccggcacg gcattgcttc
     9721 caatgatggc cgatgctccc gaacaatatg ttttatctaa acctgcaatt gctgccggta
     9781 cggccccaat atttacggta aaagtataag gagcacccgg acaaccattg gcactgattt
     9841 ctgtaacggt aagtgtatgc ggaccattca aattccagtt tactgttatt gtatttgtat
     9901 ttccaccgtt aataatacta ccacctgcaa ttgtccagtt gtaagtagaa ttgttaagtc
     9961 cgggagcagt ataaactact ccgttgagtg cattgccgca aatactatcg ggaccattta
    10021 tttgttgcac acctacaaac ggcactacat taattgtgta aaccacattg gtagtttttg
    10081 ccgggcaacc gttgtcgttt actatcacac taaaattgta tgggttggtt gatccctgag
    10141 cacatgatgg tgtccagcaa aattgtgatg taactgttga aagtcctgta gtacctggca
    10201 atacagcagg gggatttaca agtgccccat taaaaatatt tcccgaagta ttgaagaata
    10261 aactatcggc attaggatcg gtaaatgtaa tattgaaaca aagttgttgg cccgcctgca
    10321 ctgtatatgt ggtttgaggt gtagtgtttg ataatattgg tgcaggattc acggggcatg
    10381 ttataaatat taattgcaaa tctctgcgcg atattcctat caaaactccg ttgcggtatt
    10441 cttttatttc aacggctaca acataaaacc ccggcaaagg cgaaagataa cttgtaagcc
    10501 ctgttaatgt atctattgcg gtatagccgc ccacgccaaa gggttgtgca acactatatc
    10561 cgggctcgta agtaatttca ttaataggcc aattgtaaac cggtgaagga ttggggttcg
    10621 aatttcctcc agtggaaatg cccgcatatg gccacacaaa attatagatg agtaaatcac
    10681 catcgggatc aacagccgaa ttaagaatac ttacagtatc gagcgcgcat atatacggaa
    10741 ctggcgctac tgcaaatgtg ggcgaactat taataatatt tgttggtggt ataaatgcat
    10801 aataggccag ccccacatcc aaagcattaa aaacatttac aatatttcca ttgcggcagc
    10861 atcgttctac tatcaattga taacctccac ctgtggtatc aactattatt gtttcttcat
    10921 aaattccttc ttctacacat tcatttggcg taaacgaaca tgtgtcgttt gcatttggag
    10981 gcgtaataaa ttgttgattc acaagttgaa gcgtggtact taagaaaaga attttatttg
    11041 cgtttggatt gttttggtct tgcttataca cacccaaatt catgtcttga ggaagtgggc
    11101 tgctgcccgg tgcgcagtac ctgtacattt ttaatgtcac cttaaacgta acactgccac
    11161 catttacacc caagttttga taatacaaat tgccacccat gatgtgtgtg gcaaaaacgg
    11221 aaagcggatt taataaggaa ataatcagtg ctaaagcgaa gaagaaaata ttttttttca
    11281 tgatgtagat gttatttatc gaaaatagaa attgttttaa atacctaaag tacagacgaa
    11341 aatttcaacc aaaagattta atcaaagtta aacaaaaata caatgcgtag gccattgtgc
    11401 gaacaaaaaa acgaggcaaa tgttttatta caaaatcact acaaaacttc acttgagttg
    11461 aaatatttat ttcgaaatga tggcaaaaat tatttataga aacgcgaaaa aacgcccaat
    11521 ggtaaattat agtttgttgt gcttttcaaa taaatttcgc caaactctct atctgtagat
    11581 ttgccaaaga tactatccat cagattttcg atgagcaatg atgaaaaccc gagtgagtat
    11641 gtattcagaa ctagaaagca atcttcgtcc aataaaattt gctttacgtt tttcagcatg
    11701 tcattcatca tgtcttccag tttccagttt tcgccattgg ggccatgtcc aaatgccgga
    11761 ggatcgagaa taattccatt gtatttgttt ccccgtttca cttcgcgctt tacaaatgtc
    11821 aatgcatctt ctaccatcca acgtatatta tcgaggttac tggtttccat attttgtcgt
    11881 gcccacgtaa ccacttgttt tatcgaatcg aggtgggtaa catcggcacc ggcagcacat
    11941 gcagccaaag atgatgcacc ggtgtaagca aataaattga gcactttggg tttttcggtt
    12001 tttaattttt ttacactgtt gtaaataaaa tcccagttaa cagcttgctc gggaaacaca
    12061 ccaacatgct tgaaagatgt aagaccaagt cgtaacgata tttttttcct actgttgtcg
    12121 atatgttgaa acggataatt tataaaccat tgatcgggca tctcttttgt cttgttccac
    12181 ttgcccgttg agctcgatac agcaacaaat tttacatgtt gtaatttatc ccattctttt
    12241 tgcggcaagg ctttatccca taatgcctgt ggctcgggcc ggcatgtaat gtgtttgcca
    12301 aaacgttcaa gcttttcgaa tccgccacaa tcaatcaatt catattcggc aaaatgaagg
    12361 ggagtaatta gttgcataaa atttttataa aaatgaaata actaaatatt gcttttggta
    12421 agtgcgattt atttttttgc aaatgcgagc acataagaaa gtatcgctgc ttttttttct
    12481 tcatttattc ctgcctttgg tgccatctta tcaacaaggc gaggccactc aagcgcagta
    12541 aatattgccg ggttttttaa atgatgacaa cgctggcaat tggcttcgta tgtttctttg
    12601 gctgcagcca tttcatctgt taatacaggt gcaggtgaca ctgcaacctt ttttgcacag
    12661 ctcattgcaa ataccatcaa cgatagcata attatttttt tcattctcat ttgaatattc
    12721 gtcaaaaata ttttttttta cacacacgcc aaaacattca tacacaataa tgatacaaat
    12781 acgagcagct gaaataaatg atgtgccatc catcaccgaa atatataacg aagcagttct
    12841 taataccact gccacattcg ataccaaaat aaaaaccctc gacaaccgca tcgaatggtt
    12901 caatcaacat ggtgctcaac atcctgtgct ggtagcaatt gtaaaaggcg aagtggccgc
    12961 atgggcttcg ctaagccggt ggagcgatcg cgcagcatac gataccactg ccgaagaaag
    13021 cgtttatgta agcaaagaat ttcgtaacaa aggcattggc aaacaattac taaaagccat
    13081 agtggccgaa ggtgcacgca caggaaaagt aaatttgata gcccgcatca caaccgacaa
    13141 tgaaagcagc atacacattc accacgagtg tggatttacc gatgtagggg tgcttaccaa
    13201 ggtgggaaat aaatttgggc gctacctcga tgtgctgatg atgcaaaaat tattttaatt
    13261 aaactaccat acacattttt atttagtaat ttttttagta ccgtagggac gacattttgg
    13321 taataatgaa aacccatcag aacccaaagc cccgtagggg cgatattttt aatatcatca
    13381 aattccttga ttaaaatacc agcctttcaa aacagaagct tgcgtgttgt tgaatatcaa
    13441 gcattcgagt gttgggattt aaaactaagg gataaaacaa attctcttca ctaaatatcg
    13501 taaggcctta gtgcagaata gtttaaaaat attttaaggt ttatcctaaa tttaatttgg
    13561 aagttgcaga ttaaaacatt tgctttgcct ctcaaagtac ttttatatgg atacatctaa
    13621 agaacatctc gatcagctag cgcacattcg taccatgatg gaacgcagca cccgtttcat
    13681 ttcgcttagt ggcttgtctg gaatttttgc cggcattttc gcattaattg gtgcagggtt
    13741 tgctgttatt tatttaaagc ttggtttccc ttattccgaa tattggataa cacccaatgc
    13801 taacctcact tctcggtcgg ccattatgaa attttttatt gtcgatgctt cactggtgtt
    13861 gattgcatca attgttgtga gtactttact taccgtaaaa cgggcaaatc agaacgggca
    13921 acaaatatgg aatgtagctg caaaacgatt gttcatcaac atggcaatac ctcttcttac
    13981 cggtggcctg ttttgtctta ttgcttatta caggtatggc tacattggta tgattgcccc
    14041 gttgatgctt attttttatg gcctcgcatt acttaatgca agtaagtata cgcttgacga
    14101 tgtgcgttat ttagcactgg ccgaaatagt gttggggctt tttgcatttt ataaaattgg
    14161 gtatggtttg ctgttttgga caattggctt tggagtgttg cacattgttt atggtgcatg
    14221 gatgtggatt aagtatgaaa gaaattctta atcaactaat ttaacccacg attaaaaaac
    14281 aaaactatag aaatactttt acccattgat tgataaatta aataaggcat ttgaaagtcg
    14341 tgtgcgttta ggcattatgt caactcttat ggtgaacgat gaagtagatt tcaatacact
    14401 taaagaattg ctcgacacca cagacggcaa tcttgcaagc catgcttcgg cattagagtc
    14461 gttgggattt ttgcaggtgt acaaacagtt tataggtaaa aagccgaaca catcatataa
    14521 agttactaaa gacggacgta aggcattcaa caaacacctc gatgcattgg aggcattgct
    14581 aaaaaaccgg tagcattttt ttcatcaaaa aacagtaatg aatacaataa caaaaataac
    14641 agaacgttca attatacaaa tctactttgt atttcaaagc actttacatt atgagtaaaa
    14701 gtaaattaac atcgcaaatc agaatatgga taatattctt tgttatcgtt ttggtgctaa
    14761 gcggagtaac tgcttttccc attcaaaccg aaataaatta tctggccaat agttgtaaat
    14821 ggctgccaac atttttatac aactggatat tacaaattca acaaggtata aatgacagtt
    14881 atgcaaaata tccttttcta gcctatggca ccgactggct tgcatttgcg catattgtta
    14941 ttggcatggc attttatggt gtgtataaaa accctgtgcg caatatttgg attattgact
    15001 gggccatgct ttgttgcctt gcggtttttc cattaacttt tattgccggt tacattcgcc
    15061 acattccttt tttccatcag ataattgatt gctctttcgg tatcatcgga ttgattccat
    15121 taaaaattgt aaaaaacaaa atactaaaac tcgaagcaat gcaaccgtaa atattccggc
    15181 aatgctccat cacattatac tttaacagac acaaatattt ttaatcatta tatcaataaa
    15241 aaaaatgaaa agtgtattta ataaaacatt attgctgctt gtaatattta gcatagcaat
    15301 gggttttttg gaaactgcag ttgtagtgta cctgcgcgaa ttgctttatc cacaagggtt
    15361 tcagttgccg cttatgccca tgaaaaacat tatcatcatt accgaattgt tgcgcgaggc
    15421 agccactata attatgcttg ctatagttgg atacatggcc ggaaaaaaca gtgctcaacg
    15481 atttggctat ttcatttttt gctttgcggt gtgggacata tgctattatt tatttttgaa
    15541 actactcctc aactggcctg catcacttgg tgtatgggat attttatttc ttattcccat
    15601 accatgggtt ggcccggtgg cagcacctat aatagtttcg cttacattaa tttgctggtg
    15661 cctgtatttg caattacaca gcaaaaaaat tacctggcat agctggctgt catttgccac
    15721 aggaagcacc atcattattt tttcgttcac ttacgattac tgggttgcac ttttcaaggg
    15781 acaggacctt gttcaattta gcaatatgca tattccgcaa aactaccaat ggtgggtgtt
    15841 tgcaatagga caggcatttt tattttttac catttatgtt tacaataagc aatcaacaaa
    15901 actttatacc ataaaataat ttcgaattat gataaacaca ttgccgatag tggcacaatt
    15961 aaaagcgcat gttttcaata acgaaaacga acaacataat aataaattta ctttcgatgc
    16021 ggagcataaa ttaatataca atctgcttat aattggcagt ctgctttgct gcaccatgtt
    16081 tacagtgcgc atgagtgcaa caggtcattt ctattatgca tttttaatat ggaatttatt
    16141 tcttgcatgg cttccatatt tttttgccaa agcattgtat aacgatcatt acaaaatatt
    16201 tcatgcattt tatttggttt gctggctggc attttttccc aatgcgccat acctcatcac
    16261 cgaccttgtg cacctgccca acaagcatac acactggtat tggttcgatc aatctatgct
    16321 ggtaatgttt gccataaatg gattaatgct tggcgctatt tcattactta cggtgcacaa
    16381 gtacatcaac aaatttttga agcgcagatt tacatggcct tccatttttg tgcttttatt
    16441 tatgtgtgcc tatggtgtgt accttggccg tatagagcga tacaatagtt gggatattat
    16501 tgctactccc atttcgcttg caaaaaatat atggaacgat tttcggcatc ctattcaaaa
    16561 caacgatgtt tttattttta ctttcgtgat tgctattttg ctcaccatca tttatttgtt
    16621 tattgtatcg ctaaaatatc ctgaactgat tttaaaaaaa cctatttgta aaatactcga
    16681 tgaaaaataa tgtgctgata aaaattctcc acagttttgt ttatgcattc aatggcattg
    16741 tttatctttt aaaaaacgaa accaatggcc gtgtgcattt ttttgcaact gcagtggtaa
    16801 tagctgcggg catttattac aggttgaatg ccatggaatg ggttgccatt tcactttgca
    16861 ttgggatggt actggcagcc gaacttttga acaccgccat cgaaaaaatt gttgatttta
    16921 tttcgcccaa ctatcaccac aaagccggta caataaaaga ccttgccgca gccggtgtta
    16981 tggtattagc tatagtgagt attgtagttg ccgcaataat tttttggccc aaaatagtgg
    17041 catgaagatt acgaaatcta tttttaatcg tgcgataaat tgaatttaca aatggggctt
    17101 attgtttttg cgacaattta taataacctt tacaccacaa gcacaatcaa aataaagtaa
    17161 tcgcaaactc cttgcaaggg ggagtctgaa atttgaagta aaaggtttga acactattca
    17221 ttgatacttt actatagatg tagattgtgc gggtggctcc tctttagagg ccgggggtgc
    17281 ccggtggcta atggtttgta tattgtacat ttacaaacag aaaaggagaa gctgagtgag
    17341 aagtttgtga aagagtaaag aagaattatt attaacgaag tgaatacatt ttttcaaaaa
    17401 tgaatagtaa tcaaattatt tcttttcagg agttgacatg tttaggtaac ttcacatttt
    17461 ctaaagtaaa taaataatgc gatggcacgt ttttattttt ttcctttttg ttgtggcaat
    17521 tagtaatgcc cagaaccata acgctgtgtg gtgctttggc gatagtgctg gtattgattt
    17581 tagtttacca aatacgccta cagcgtttga gacaggttta cgtacaagag gtagctgtgt
    17641 taatgtatgt aatagtaatg gtttgctttt attttatgca aataccagag ctgctacagt
    17701 tggtaaaact actttggttt ataaccaaaa tcatcaattg atggataatg gtattaatat
    17761 tttaggatca ggatggtacc atgaacttgt gattgtccct tcaccacttg attctaactt
    17821 gtattatcta ttttgtgttg gtgtcacctc aaattatggt ttagaatatt ccttaataga
    17881 catgtcacaa aacaatggta tgggcaaagt gatattaaag aatgtacaaa aacttggcag
    17941 ccagcaagta gattgccttg cagcagtaaa gcatggaaat ggtcgcgatt ggtgggtggt
    18001 gggcaggagt ttaaattttt cacaaggaca atcgagcaat gaattttgga agtacctcat
    18061 aactcctacc ggaataagtc ctgtaatgta tcaatcaatt ggatcgttaa atagtactgg
    18121 gttagcaaaa ataatttttt caccaagtgg cgatactttg ttatttagca atgctggcga
    18181 tttgcttgag tattataatt ttgataggtg cacgggcatt ttatcgaaca cagttacaat
    18241 aaggccacag gcaacagtgt atagcgaatg gcaatggggt gtggagttta gtgcaaatgg
    18301 tagatttata tatgcaagca caagcaaaga cactacacat ttatttcaat acgatttatg
    18361 gtcggccaat ccattggcta cacggcaaat gatatgggag agcacctttc agccatacac
    18421 cggtggcgac cttaaacgcg gccctgatgg gaaaatatat ttttctaatt gctgggacga
    18481 tggtactttt tttccttatc catatcccga tacagcctat tacttgcaga acacttactt
    18541 atctgttatc aactcacccg atagtcttgg cgctgcatgc aattttacac cttacagttt
    18601 ttaccttggt ggcaagcgta cttactgggg cttgcctaat aatcccgatt atgatttggg
    18661 gccgctggtg gggagtccgt gtgatacgtt gagtgtgaac ctcacccccg gcccctctcc
    18721 ggtaggagag gggagcctct tcctaacata catatcggca tgggagaagt tgtttattaa
    18781 tgcaagtgga ttgagaggga agcaattaac ggcaaccata tacgatgcta ctggaaaaga
    18841 aaagcttgca aggggaaagc ccccaggtgg gggtttgggg gtttttggcg ggcattttac
    18901 acttgatgtt gattgcacag gttggcccag tggtttgtat attgtacatt tacaaacaga
    18961 gaaggaaatg ttgagcaaga agtttgtgaa ggagtaatac catcagcttt aataaataca
    19021 cgtgtaaatt atcttacaac taaaaatcta tcctccttat tcgttcaatt atttgagtgt
    19081 gtatttcctc taccgataac attttatttt catcatcata acatgttatg ctggcaatat
    19141 tgggatacga ttgggtaatg atatcatact ctttttttac cgaagcctga aaagaaaaat
    19201 cgctttcgtg aatgtcttct ttgccttgca aataatcgcg ttcgttgcca gtgcgttttg
    19261 ttgatagcga agtttgagta aatgtaaaag gcacatcgag gtaaatggaa agttcgggtt
    19321 ggggtatctt atacaattga aattcgtaat ttaaaatcca atcgcgcaaa atattttttt
    19381 cagtttcgtc tttcaatttt gcacattggt aagcaatatt cgaaagcaca tatcggtcta
    19441 ctaaaacata atacccatca tctatccatt gaagcatttg aggtgcaaaa tctatacgat
    19501 cgcgggcaaa aagcaatgcc actaattgcg gatgcacatc gcctacatta ccaaactcgc
    19561 cacgcaaaaa ttttgctatc aactcaccat aaactccaac accactgcgg ggaaaatgta
    19621 taaactttgt tttgcaatta tgctgctcga aaaattttat cagcaatgct atctgtgtag
    19681 attttcctgc accatcaagt ccttctatgg caataaattt tggaaacgac atttttattt
    19741 taatactatg gacaaaaaaa attaactaat aagcgcgggc aaaaataacc cgttgcgatg
    19801 atggtttacc ggtaagtata cacttgcctt cttccatttt attgttgagt ggtatacaac
    19861 gtatggtagc ttttgttttt tcttttatag catcttcagt ttcagaagtg ccatcccaat
    19921 gtgcatacac aaagcccggt gagttgtcga gcacggcaac aaaatcgtcc catgtatcaa
    19981 cataatgtgt agcactttcg cggtggtcgc tggccttttt ataaatatta tcctgtatct
    20041 gtatcaataa attttcaacc ttcacatcta aatcaacaat agaatatgtt gctttttctt
    20101 tggtatcgcg tctggcaatt tctacttggt tgttttccat atcgcgtggg ccaacagcaa
    20161 tgcgaatggg cacacctttg aattcatatt ccgaaaattt ccaacccggt ttataagtat
    20221 ctcggttgtc atattttacc gaaatatttt tttgcttcaa cgattttata ataggatcca
    20281 caaatgcatc tacttgttgc aattgctctt cgcttttata aataggtaca atcaccactt
    20341 gaataggtgc aagcatagga ggtaaaatta aaccatcatc gtcactatgt gccattacaa
    20401 gtgcgcccat caatcgcgta gaaacgcccc acgatgtagc ccaaacaaat tcttgcttgc
    20461 cttctttgct cgtaaatttt acatcgaatg ccttggcaaa attttgtccc aaaaaatgtg
    20521 aagtgccggc ttgcaaggct tttccatctt gcatcaatgc ttctatgcaa taagtatcga
    20581 gtgctccggc aaaacgttcg ttggcagttt ttacgccttt tataaccggc acggccatat
    20641 acgtttcaac aaaatttgca tacacatcaa gcatcagttt agtttcggca atggcttcat
    20701 cgactgtggc atgtgcggtg tgtccttcct gccataaaaa ttctgttgtg cgcaaaaata
    20761 atcttgtgcg catttcccat cgcaccacat tggcccattg atttataagc aaaggcaaat
    20821 cgcgataact cattatccag ttgcgatagg tattccaaat aatggtttcg cttgtagggc
    20881 gaactattaa ttcttcttcg agcttggctt cgggatcgac tataatgcca ctgccatcgg
    20941 gtgcattttt taatctgtaa tgtgtcacca cggcacactc ctttgcaaag ccttcaatat
    21001 gggcggcttc tttacttaaa aatgacttgg gaataaacag tggaaaatac gcattgctat
    21061 gtccggtatc tttaaacatt ttatcgagca cctgttgcat tttttcccaa atggcgtagc
    21121 cgtaaggttt tatgaccata caaccccgca cggcactcga ttcggcaagg tcggcacgat
    21181 acacaagttc gttataccat tccgaataat tttcgctacg ctttggaaaa tctttagcca
    21241 tacaattaag ttattatgaa tatagaattt agtatttatg aagtactacc cgccttaaca
    21301 ccttcaaagc aaaatagata gtaatatggc agcaaaattg agaaagataa tgaaagaaaa
    21361 aaattgttat gataaacaat ttgtaatttt gctcatctaa atgaaaatga acgtcaatca
    21421 attctctata gttatgaata caattaaaaa taaaaacaaa tcaattatga aacaaagcat
    21481 gttgatactt tttacatcgg ctgcattatt tgccggatgt aactcttcgc aacaaactgc
    21541 tggcggcaac agttatgacg acatatacgc tccatcgagt gtgcctgttg cagaaaataa
    21601 ttcgcaggca ccacagcaat ataatcaggc tgcaccacct acaaattata gcaacgataa
    21661 tagccaacca caaaacagtg gcagcgataa tcgttttgat tatggtaaca aaaacggtaa
    21721 cgataacaac cgctactccg atcaaaatgc gcagggtggt ggcaacgtaa ccaataatta
    21781 ttataacgat gatgattatt atgattatgc atacacttcg cgcatgcgca gatttcatcg
    21841 cccctttgga tggagttatt atgactcgta ttataccaat tcattttggt acgataataa
    21901 tccattcaac tatggccaaa gtatatactt aggatacaac tggtgggcac cttcttattt
    21961 ctatactaat ccgtggtttg gttattcagg ctttaattgt tggaatagct ggggcaatgg
    22021 tggctgggga atgaattatg gctggggctt tggttgtaat aatttctaca atccttattt
    22081 tggtgggttg ggatatcaaa atggatattg gaatggatat aaccaagggt attgggatgg
    22141 atattacaat ggcttgtatg gcaaccaaat gaatccttac tactataaca gctttgataa
    22201 tgtagtgaac gacaataatt attacggatg gcatggccgt gagaataaca ccattaagaa
    22261 caatcttgtt ggcactacag taagttcatc accacgccca attggagcaa tttatgctga
    22321 agcagtaaac cctcgcagca acgatggcaa tgtgcaatca aatcctgcac ctcgccccgg
    22381 caaaagtgtg gtggatgtac cagcaccccg cgatgtgaaa agcaacgaat accaaagcac
    22441 accgcgacct atgcaaacta ctacaccggg ccgtggcaac aatgtagata attatacagt
    22501 agaacctcgt cccgcaaaag gaaacacgca aggtgaaaca gtacccaata atggcaacaa
    22561 tccgcgtgga aataacaata ctgtagagcc tcacccaaaa ggaacaggaa cagttgatcc
    22621 tcgcaataat aattcgtcac cgcgcccaca gtatgaaaat gcaccggcaa ataacaatac
    22681 accacgcccg cggtacgata acaacaatgc acctgccaat aataatgaag caccgcgccc
    22741 gcgttacgat aacaacaatg ctcccgccaa taataatgta acaccgcgcc cacgcaataa
    22801 cgatacacct cgtaccgagc ctgcaccacg cccttcgaaa caagataagc cacgggtaca
    22861 gccaaggcag cagtcgcaac ctgcaccacg tcagcaacaa gcaacacctg cgccacgcca
    22921 gcaacaatcg gcaccggcac cacgccaaaa taacaatggt ggctccagac ctcgcggtcg
    22981 taactaatag caactaatat taaaggcatc ttccttatcg aaagttttta ttcgaaagtg
    23041 agatgccttt tttatgcctt tttaaaactg ataacgcatc acaaaatatt tttctcaatc
    23101 aaacaaataa aaatctcaat caataaattt atgaaacaaa taataatagg tgtcgccaca
    23161 tttgcaatgc taactgcaag tgtatacgca caatcgttgc aatcggctat cgatttcaat
    23221 caatcgctgc cgggcagtac cgcccgttcg ttggggatgg gtggtgcttt tggtgcattg
    23281 ggtggcgacc catcgtgcct catggttaat cctgcgggcc tggccatttt tcgcaaatcg
    23341 gaattttctt ttacgccaac atttaattat cgtggtaact cgagtaccta cttattaaac
    23401 aatgtaactg ataatcgctt ctcgtttaat atgggcaatg ccgctgctat tttttcgggc
    23461 tatcgcgaaa atcgtaaaga ctggaaaggt ttaaattttg gtatagcata caaccgcgat
    23521 tattctttta gcaccaacaa ttactttgaa ggattcaatt caaaaaacag catattgaat
    23581 tcttatcttg ataaagccaa tggcacaagc gattcacttt ttagcgactt gaattttgcg
    23641 ttcgatgtgg ggttggccta caatgctttt ttactcaatc ccgatacggg gcttactaat
    23701 atgtatacca tagctgcacc aaacggtggt gtgcaacaaa gcatgactca ggagttgcgc
    23761 ggtggcaaag gggcgtggag ttttgctttc agcaataatt atcaggataa aataatgctt
    23821 ggtggctcgc tcgatatttc gagcttccgt tacgatgaaa caaacaccta taccgaaagc
    23881 gataagtacg attctatagt tagcccttat gataaaatgg attttaaatc cttcaccctc
    23941 aatcaggata agcaaacaag aggaagtggt gtaagtgcga aatttggaat tattgtaaag
    24001 cctgtagaat ggttgcgtgt aggtgctgcg tttcatactc ccgttgttta caatatgaaa
    24061 tacaatgacc agtatagtat agcaagttcg tttgcaagta aacaatatgc tcccgatgcc
    24121 atagaaagca ccttcgatta taaatatact tcgcctttga aagccgttgg tagtttagca
    24181 ttagtatgga agcaggttgg tttgttgagt gttgattatg aatatgtaga ccatcaggcc
    24241 acacgcttca aatcgaaggg ctataatttt gtgaatgaaa attcggctat cagcaaacaa
    24301 ttacaaggcg cctcgaacgt gcgtgtgggt gccgaatacc gctatcagat atttgccgtt
    24361 cgtgccggtg cagcaatgta tggcagccct gtgcgcagtg gtaatttaaa aggtgcggcc
    24421 gattatgcag cagttacata caccggtgga gtgggtatgc gtgagtcagg atattatttc
    24481 gatattggtt atggttacac aaaaaattcg aaaacaacgg caccctatac tgttgccgaa
    24541 caaaccacaa atgatgtagt tacagattat aaacttcatc gtatacagtt tacgttaggg
    24601 ttcaaatttt aagaaagcat aaagcaataa tttataaaga gccgcaaatt tgtttgcggc
    24661 ttttttttat ctccatataa tgaaagcata attaagtata aatttgttgt ctatgataat
    24721 actgttttga aagcaataat gagatacatg aatttgaaaa ccgaaaatca tatattgtac
    24781 attcacaaaa taatagcata gtcatgttcg aacaaataaa tgcaaacgtt acaaagtgta
    24841 ccacattttc gcaagaagag cttaatattt tcgatgccct tttagtaagc aagacctatc
    24901 ccaaaaaaac ttttctgttg caggaaggtg aagtttgtaa tttcgaagct tacgtagtaa
    24961 agggttgtgc acgtactttt tatattgacg acaatggctt tgaagtgata ttgcaattcg
    25021 ccatcgaaga ttggtggata agtgatattg ccagttttaa tgaccaaacg ccttcacgcc
    25081 tttttatcga aacaatggaa gattgccagc tgcttttact tgcaccacaa accaaagaag
    25141 atttgctttt tcgagtgcca aaatttgaac gcgtatttcg catgatgata caacgtaact
    25201 tatcggccac tcaaaaccgc ttgataggca acatagccca gcctgcgcaa gaacgatatt
    25261 tagcattttt aaaaaagtat cccaccattt cgcaacgcat accacaacat tacattgcct
    25321 catacttagg aatatcgccc gaatttttaa gtaagataag agccaaaatt gcacgaaaat
    25381 aatgactcca aagggagcag aaaaatcgcc cggttttttt atacaaaagc gcaaaacaaa
    25441 tttgcataaa tctgtgttat ctgtgagcga taaactgcaa agcagactag tgttatttat
    25501 gaaaaacatt ttttagcaat aacaattggt gtactcattt cttaatctag ttcaatggat
    25561 ttttattttt cacactgcat ctttgccaca taaattttta ccaacataca atggatagaa
    25621 aagatttttt aaaaaaggga ataatgggtt caagcatttt tgtagcatcg gctgctatgg
    25681 ggcaagtgct aaaaaataat attgatgaac tggcagaatt gaaaactatg ccaatggaaa
    25741 aagatttcgt tggctttaat catataccaa acacaaattc aaaaataatg gcgaatacag
    25801 ttttacacaa agccgataca cgcggtaatg ccaatcatgg ttggttaaat agtcatcaca
    25861 catttagttt tgcaaattac tataatcccg agcgtatgca ctttggcgtg ttgcgtgtgt
    25921 tgaacgatga tagggtagaa gcaggtatgg gcttcggcac acatccgcat gataatatgg
    25981 aaataatttc gataccgctc gaaggcgatt tggaacataa ggacagtatg ggaaatgtag
    26041 cagtaatacg caatggcgat attcaggtga tgagcgcagg gagcggaatt actcatagtg
    26101 agtataataa aaataaagac agcgaagtaa aatttttgca aatctggata ttccctaata
    26161 agaaaaatgt tactccacga tacgatcaaa ttagtttgaa ggctcaagat cggcataata
    26221 aattacagca aataatttcg ccaaatgcaa atgatgaagg tgcatggatg catcaggatg
    26281 cctggtttca attaggcaca ttcgacaaaa attttgaaac acaatacaaa ttaaagaaac
    26341 aaggaaatgg agtttatgct tttgtgctga agggtgatgt aaccatagaa ggtacagaac
    26401 tgaacgaacg cgatggactc ggaatatgga atacagaaaa catttcaatt aaatcgaata
    26461 cgacaggtgc cgaaatatta ctgatggata taccaatgaa tatttaataa aatttaaaac
    26521 aaaaaaaatg tcaaacacaa actgggctct cgacccatca cactcgcacg ttggcttcaa
    26581 agtgaagcac cttatgttta caaatgtaaa cggaactata ggaaagtata atgttaccgt
    26641 agaatcgggt agcgatgatt ttaatgatgc acaatttgct tttactgccg aagtgaattc
    26701 aatatccaca ggcgaagctg cacgtgatgg acatcttatg ggtgccgatt tttttaatgc
    26761 tgaaaaattt cctcaaatta atttcaaatc gacaagcttt aaaaaagtaa gcgaaggtaa
    26821 ttatgaactt gtaggcaatc tcaccatgct cgatgtaaca aagccggtaa cacttgcagt
    26881 agaatatgca ggcatgatga aagatccctg gggcaatatc aaaaccgctt ttgcaatttc
    26941 gggaaaaata aatcgtaaaa attggggttt aacatacaac gctgcactag aaacgggtgg
    27001 tgtgttgata ggcgaagaag tgagtattaa tgccgaaatt caaatggtaa aaaaagcagt
    27061 tgaagccacc acagaagtag tagcttaaaa agttactctc tccaaataag tgttaatctt
    27121 aaaacataaa ccccggcatt gtcggggttt ttgttttaat aaaactttgc atttgcattt
    27181 ctttgtattg attaatgaag caattttttt gttgggtatt gattagtgtc atagacttac
    27241 gcaatgatta tttttttaat gtaaagtgtt cttgtgccgc acttattact tgaactttcg
    27301 atatcattca tcttcgaatt tctgaacaac atgattgatt cttttcatga aagtaaaaaa
    27361 ataaatggcc acaatatcag cgcaaaacca tgtctcacaa aacaataaaa atgatgtgtt
    27421 tattacttga tgaagttttg tgcctcagtg cttttgtggc aaaaaactat ttttaatagc
    27481 ggactattga ttgaaagagg tgactaaaaa ttttggaact gaaatttacg aatcgagatt
    27541 ttatctgttt tgtttttaca tcaacgattg gaataaatat ttcgcaatta tttttcggca
    27601 gcatatttgc tctataaata ttgtatcacc cctgttttta ttttcgcgat ttataacgtt
    27661 cgaaaaaaaa tattactcgt ctcaaaaaat ttaaatacaa gacagttcat cgttaaatta
    27721 tgccaccaat aaggttgtgg caatatttgc aaagtgcagt tttttatttt ggtttttgaa
    27781 tttttatttt ccaattaaac accttcttgt tatgaagcag ctaacattaa ttgcctcttt
    27841 tttggggctg actttctatt gctcattttc gcaatcgaat tgcgtaacgc acaaacaaat
    27901 acttgtatgc ccgagtatta tgttatacaa cgaaaccaat gtaggacaag gcgataatgg
    27961 tacttcgcct tgcaatattg cgggcgaaga tgtggtgtac gaaattctta cacccaacgg
    28021 agcggacata ttatttgtta cagttgacaa tgcatccaac ccatttttgg tttatctcga
    28081 agaacaaaat tgtggcagtg gaatttgtca ggcgtttaca ttaggtgccg gaaaaactaa
    28141 tttaaaaata agcgtcaact cctatcaaaa atattttttg tggatcgatt caccatttaa
    28201 aatcacctac aacataagtg tgggtgccga tacgggttcg gtgtatgtaa acattcctgt
    28261 aacaaaaggt aatttgcaat tagatgccag catgtgcgct actccgccat ttgcagctaa
    28321 caaacctttt tttcaggtgg catacaataa tatttttcaa accaatccaa tgacattgtc
    28381 accacttttt attcaaggca acatgtgtgt ttctgtattt tttaaaaaca ccacgggcat
    28441 tgaaggtgta aaaaagttca ccttttattt cgatccaaac gggtatgcta atgtaacagc
    28501 gcctgcatta attacgggat tttataatgc aggaaattgg gtaggaacaa aaagcaataa
    28561 tgtgtggact tacatttttg acgattcgct tggcaccgga agtggcgatt ttacaggtac
    28621 tcccgactca tgcctggctt atagtttttg ttttgcatta acaccgttat caaacaatcc
    28681 tgttcttaca aatgtaatag ttaaaattga aggcgatggc aaaggctcag gcttttcggg
    28741 ttttgtgcat tcgggttgtt gtccggtagg ttttagtggt tgtaacttta gtggaggtgg
    28801 cggaggtggt ggaaatgcca gcgcatttgc ttttggcttt gccgatccgg gtggttcgct
    28861 gccattaaaa attgtttctt tcgaggtcaa gaccaatcaa aataatgcac aactaaaatg
    28921 gatggtaatg aacgatggcg caaatatttt ttacgatgta gaaaaatcag aagatggatt
    28981 gcaatggagt acaataagca cagttaaaag taaaccatca tcaaaagagg ttactgtata
    29041 cgagtcaatt gataaaaatg tacagcaggg aaataatttt tatcgcataa aatatgagga
    29101 tgataatggt gagattattt tttcgagcgt tgaaaaaata tttgtatcta caatgcattc
    29161 gatatcggca tttcccaatc ctgcaaaggg attaattaat gttagtggat ttgaaggtca
    29221 aataataaat gtaaaaattt ataatcttga ggctaaggaa atgctatgta aatatacttt
    29281 taccaatagt ggattgttta tcgacagcag taatttgcca acaggttcgt attgctttat
    29341 ttttacaaat caatatgaaa ccgaacgcaa aattattgaa gtaataaatt aagagtccaa
    29401 attcaaaaac aggagtggaa taaatactat ggtttacaaa atgagtctgt ttgagactat
    29461 ttcatcatca gttgaatatt caaaattaaa tcgacttcgt tgctaatagt aaggcctccg
    29521 ccttcgagcg tattgttcca cattaaacca taagccaatc tatttatggt ggttgtggct
    29581 ttgaaaccgg cttttttatt tccaaatcca tcggttgctg tgccgttgaa tgtaacatcg
    29641 aaggttactt gttttgttac atcctttata gtgaggtcgc ctgtgagttt gtattttttg
    29701 ccggcaattt ttttgaacga tgtactcttg aaactcatgt ttggaaattt attgcaatcg
    29761 aaaaattctt tgctgcgcaa ttcaatatcg cggtcggcat cgcgtgtatc aacgcttttt
    29821 gtttctacat tgaagtttat ttctgcatcg gcaaaggtga tgtctttcga tttaactgta
    29881 cccgtatacg aagtaaacat gccatctatt tgcgaaatca taagatgcac cactgtaaat
    29941 tttaattgtg aattgtgggt gtcaacaatc cagtttagtt gtgcctgaat ggtaaagttc
    30001 atcaccaaaa gcaacgttgt tgatagaaga agttttctca tattatttat aaaatttaaa
    30061 gttgaaacta attacaaagt cattcaattt tacaaccact ttcaatttgg acttacatat
    30121 tgcaaggtgc tgaatttatt tttgtcgaac acttcgtttg ccacgtgcaa aatatcttcg
    30181 ggagtgatgg catttatttt tgcgaaaatg gtttcaagcg tatctaccct gttgtgattc
    30241 aacaggctac ggcccaatgc catcattatg cccgctttat tttcctgtgc aagtgctatc
    30301 tgccctatca gttgtttttt atgttgtgat aacttggttg tagtaaattt attgttgcgc
    30361 aatttttcca gttctttatt tacaaccgca taagtcttat cgaataattt tggttctgtg
    30421 gcgaagtaaa tgttaaaaac acctgtatcg ctatacgaaa ggtagttgga ttctatgttg
    30481 tacgtatatc catatttttc gcgtaccgat aaactcagaa tgctattcat agccgggcca
    30541 cccaaaatgt tatttagcaa ggaaagctgc agtcttcttt tatcacgaaa cgaataggca
    30601 acattgccca tcacaaaatg agtttgctcg gtaggccgct ccattgtttg agcaaatgtt
    30661 ttattgtgtt taaacttttg cactgcagct acattgtttg cttttgcacg cgatacaaaa
    30721 tatttatcag ccagttcttt tgctttggta aaagaaatat caccggttac actcaccacc
    30781 attttttcgg gatgatagtt tgtttttata aaattgataa tatcatttcg ggtaaaagcc
    30841 ttcacacttt gagtagtgcc taaaatggga ttacccaatg tattgttacg aaataatttc
    30901 gattcaaaat cttcgaaaat ttgctccgaa ggattatcaa ggtacgatcg aatttcatcc
    30961 aataccactt ctttttcttt ggcaatttct ttttcaggaa atatagaatt gaacatgatg
    31021 tcggcaataa ggtcgaaggc gcgctcaaga tactgcacct gcaccgatgc atgtatacac
    31081 gtttcctctt tcgatgtata tgcattcaac tcgccaccaa ctacttcgag cctgttaagt
    31141 atttgcatgg aagtacggtg tgaggttcct ttaaaaagta catgctcaat aaaatgtgct
    31201 aacccttcct tgccgtgcga ttcatgacgc gtgcccgcat ttaccaatat accacaatgt
    31261 acaatttcgc ttttgcctgt attttgatgt atcagcctta taccattttt taaagtggta
    31321 atagaaaatg gcggttcgtt ataactcata ttaagtgcgt atatagtata tgttgtatgt
    31381 taaaaatcca tttcgccacc cgggcgttcg ttttgtttat cgttgtttcg ttgttgcttt
    31441 tgtttatcat caaaccggcc aaagcgatag ttaaatgtaa aagtaaggat acgtgtatcg
    31501 cgcttacgtt caaattcaaa atcgaaataa gtggaagtat tacgaacctg aaatattctt
    31561 gtatcgaaaa tatctgccag gccaattgta aggctatact tacttttcat ccagtctttt
    31621 tttatagcaa catcggccga gtaaaccggc tgcaattgac cttgtgctcc ccgcattatg
    31681 ctattgtaac ttcccgacaa ctgagctgtc aatgtttttt gcaatttaaa attattgttc
    31741 atacgggtgc tccacgaaaa ccctttacgg ccaaggttct gctctatgtt gccggcattt
    31801 atttcctggt tgaaaaagtt aacattcagc agcacattcc accattttgt aatatctccg
    31861 gtggccgata tttctacacc ataatctttt cgccatgcag aatttataaa tgtcataacc
    31921 gaaaatccat cactagttac tttgcgaaaa cgctgaatgt gattttggtt ataacgatag
    31981 tacaacgttg tgtttagagt tgtgaaagaa aatattcttt tgttcgatag ttcgaaagag
    32041 tgaattaatt cgggtacaag attcggattg cccactctga tattttgagg atcgctaaag
    32101 tcggtaaagg gattttgttg ctcggcattt gggcgttgaa tacgcttgcc atagctggcc
    32161 ccaatttcat tagtggcact tgaattatac cggaaaaaaa cggaagggaa aaaatgatta
    32221 aaacttcttg aagtgctctc gctatcttta tatttaaact gattaaaagt attttcgttt
    32281 cgtaaacccg cctgcaccga tactttacca atactataca aacccataac atatgctgca
    32341 gttatgtttt cggtaaagct aaattgtgtg gaactggcac tcagtatatg gttattgtta
    32401 gtaaactccg attcatattg attgagcgag ttattgcttg tgcggccaaa gtattttgct
    32461 ccgccatcaa tttttatttt ttttgttgca cgccatgtaa catcactttg tgtatttaaa
    32521 ctttgcaggc cattgcccac cttaaaattt tgaagcatcg attgcgattc tacaaaatca
    32581 cgaaacccat cgaaaaattt aatggtcgca tcctgattgt cgctgcgtgc attgcgcgaa
    32641 taggtagcat ctgtattcca ttccacttta ccggcagcaa acgatttgcg aaatccggca
    32701 gctatatcat aactccagcg ccaatcattg ccagcattag tgcgcaattg ttgcgataca
    32761 aattgctgag gataatttgg ttgcaacaaa aaattatcat agtagatttt ttcatcgcgg
    32821 ctaccatcgt tgcggataaa attaatagaa gtagtaaccg atgcactttt tgacaactga
    32881 taatcgcccc ctgtacgaaa tgtatgcgtt gtttgttttg taatattgcg actcgactgc
    32941 tccagtgtaa aggcagtgtc gacaaaagtt gctgtgcggt caagtgttcc aatcattagg
    33001 ttacgggcat ctcttatggc atacgatgcg aatacattca gctttttgta catggcattg
    33061 atgccaacat ttgcgccata cttgttgcgg gtagatacat ttaccgaagc attaccatta
    33121 aatccctttt gaacattttt tttcaaaaca atattaatta tgccccctgt accggccgcg
    33181 tcaaattgtg atgatggatt tgaaataact tcaatccgtt cgatggaaga tgccggaagt
    33241 tgctcgagtc ccgattgatt atcaccacca agcattccac ttgggcggcc atcaacaagt
    33301 atttgaagat tatcgctgcc acgcatattc acattgttat cctgatccag gtttatggcc
    33361 ggtatttgtt ttaatgcatc ggatgcacta cccccgacag caaccaaatc tttatcaatg
    33421 gtataaacct tgcgatcgag attattttgc gcatagtttt tttgacccga tacttctact
    33481 tcgtttagct gagttgccgt tggcgataat tttattttta gtttacgcgt tttattcgtt
    33541 acatctaaat tgaatgtatc ggaaacaaaa gtggtgtagc ccaaaaaact tacccgcaca
    33601 taaaaagtgt ttgcgctggc cttaaatgca aaagttccat tatcgctaac aagcattgtt
    33661 gccaatggca atgtatttcc cggttgcatt aaagctgctg tcgaaaacgg tacaggtttg
    33721 tttgtatttg catctacaac caatccttca ataccggaag ataaaccttc ccctataatc
    33781 tgagcgacag aaaagaactg tatgaacata aaacagcaca ataactgcga agcccttaaa
    33841 agaaaaatta atctatgtga cattaaaaaa aataagattg cgaaagtata aaataaacgc
    33901 attcataaag gtaaaccccc aatttcatta agtttatgat tgcaaatccg ttgttgtgct
    33961 tatcaggcaa tgctaaggtg aacagtttta atgggtaagg gttttttcca atgtagaaat
    34021 aaatgtttcc atttgatgcg aataaataaa ggtggaattt aataggattt tgtatatcaa
    34081 tttaattttg atgcttttgc tgcaactgtt caataaagtt atacttaatg tcatatcatt
    34141 ttgaaggcgc agcatggttt ctattcgatt ttttttataa gtttttgcga tgctgtgtca
    34201 ccaaaactaa taatgtgata gcgcttacat aaaagttatt aacaccgttt aatggcccat
    34261 gtgtaaaact tcaacaacaa atggcttaat taagggcgct tttttttctg ctttgttgtt
    34321 gggaaaaaaa attaattcta aattgcacaa cttttctaaa gctgaaatta gaaaagaaat
    34381 tggttcctcc cctttttagt ttgtttaacg cgtgtttttt gtaaatgatc aaattgaata
    34441 tacaatttgc tttatttaat ggtactttgc ttagggtctt tttgaaggct ttgcattgct
    34501 ttgtcctttc acatcagtcg caatttcact cgttttcttt aattcctttc ctcaaaaaat
    34561 attaaataga aaaatcaatt aaattatcta cataatgaat agtaacatct acaaattaga
    34621 aatgccaaaa aggttaagtt tggcaacaag attatttacc gcactgactg ttttttgctt
    34681 gttgggattt tttgcccagg cgcagtttgt aagcattcct gtaacaggtt tcaataatga
    34741 cattgttgcc agtggagtag ggccgggtac agctttgtct atgccaggca cctcaaatcc
    34801 aactattggt gtggatggtg caggatttac atttattgac aatacctata aatggcaggc
    34861 agcaaatgct tttccaactt gctttatgcc ggcaagtggt tcggccccaa gtttggttac
    34921 aaccggctta acttataatt ttcaaagtta taccgctctt aatgctttaa ctttggacaa
    34981 taactccacc acctatctta cttcaccttt tccaagtatt gggcagttaa cattaacaac
    35041 gccagccagt tatactagac tatatgtttt atatgagtcg gtagtaaata taggcccaag
    35101 ttattttata aatgccacag taaaattcac ggactccact tcgcaaactt tttccaataa
    35161 cacagctgta aattggtttt cgggtactcc ggcagttacc aatgtagcac gagcgcaaaa
    35221 tgtatctcca ggtttgggta ccacatgccc aatagcacct aaccttttcg aattgccact
    35281 cacattgagc gctgccaatt atggtaaaca agtagcttat ataaccttta ccattcctac
    35341 ctctttaaca tcaggtacta cacccgatag agtaaattat tttcacgcta tggcagttgg
    35401 aggcttattg gcttgttctg cacctgcggc acagcccaca ttgccaagtt taactactat
    35461 ctctaccagc caaatcaatg gtagttttac agcagcaacg ggcccaccca gtggatactt
    35521 agttgtgaga tacccgaatt tagctgctga aacagctcct gtaaatggca caacttatgt
    35581 tgcaggtcaa tcgttgggct taggtacagt tgtgcaatca tcagcattaa ctacgtttaa
    35641 taatacagga ttgtttggag gtactaccta cgattactat gtatattcat tcagcactgc
    35701 tacagctcct tgtggtggtc ccatttattt aacagcgggt ggctctattg gcagcgctac
    35761 tactaccgct tgtagtggtg tggcgggtgg aacatactct gtagggccta cgggtactta
    35821 cctaaccctt accgctgcgt tagcagcagt gaatgctgga attaccggac cggttattct
    35881 ggaacttcaa agtacataca caagtggagg cgaaacattt ccaattactt tcccttctaa
    35941 ctcttgttta ggtcctatta acacattaac aattaggcct gagttgggtg ctgcagcact
    36001 tgccattacc agcgccaatg taacaggaac aatcaacttt aatggagcta aatatgtgac
    36061 aatcgatggc cgcccaggag gtgttggcac ttcgcaatta actattgcca atactaatgc
    36121 aggagcaagt tatgctgcca attttgtggg cgatgccact aataacacaa taatgtactg
    36181 tactgttacc agtagaaata attctgctac cagtggtact attgcattta gtacaggtat
    36241 agttactggt aatgataata ataccatcga taattgtatt attagtgatg atgcaaatgg
    36301 cacatcattc ccggtaaacg gaatt
//
LOCUS       DAORQA010000012        35075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_261_length_35075_cov_45.448886, whole genome shotgun sequence.
ACCESSION   DAORQA010000012 DAORQA010000000
VERSION     DAORQA010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 35075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35075
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_261_length_35075_cov_45.448886"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..>1427
                     /locus_tag="PK736_02090"
     CDS             62..>1427
                     /locus_tag="PK736_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32215.1"
                     /translation="MTKFSKIFLLLVLLSGSCFSQDFYLPIANSFNLRWAPYINSVNS
                     TVHTGIKPYRSAELNSSCNIDSLIHLPVNMKGITQTRAGRKIFNEAVVKVNGEDFNFY
                     LNPVLDISLTRENTYKRNLLTFSRGIQLRGNIGERFSFFTQFNENQNEYPAYVDTAIR
                     YSNVVPGLTRPKGIYGKRDFAIATGTISYSLKKYFNFQFGHDKNFIGDGYRSMLLSDN
                     SPNYPFLKITTTIWKVKYMNLFAVFQDLKVQPPSNYNPEDFSFRKKYGSFHFIDFNIG
                     KRLSIGIIEAVIWQHDSIRAKGFDINYINPFIFFRPVEFSMGSPDNAMMGVNAKFKLS
                     SHHILYGQFLLDDIKIDFLRDSSGYYGNKFAYQLGYKTFAPLGIKNLSLLTEINGATP
                     YTYQHRSSITNYAHYNQALAHPLGANFIEWVAMVDYHFGRCYFRLENIMAKTGQDTGT
                     INFGG"
     assembly_gap    1428..1437
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1438..1666
                     /locus_tag="PK736_02095"
     CDS             <1438..1666
                     /locus_tag="PK736_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32216.1"
                     /translation="YYTPFKFFGNKTLQGNLTKINNLGMTFSYFINPSYGLNIFVNMK
                     YRKFENNKQSANDMFISFGIKTELYNSYFDF"
     gene            1862..>2860
                     /locus_tag="PK736_02100"
     CDS             1862..>2860
                     /locus_tag="PK736_02100"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRC32217.1"
                     /translation="MINIIFASITAFIITFLAIPAVIKIADIKGLYDTPGGRKLHKAS
                     IPRLGGLAIFAGVAFSFAFWTAVFKFEPVQYILASMIVMFFIGIKDDIIEMPASKKFI
                     GQLIAALIIVLFGDIRITSMCGVMGIYDIPYYVSAPFSLLLIILIVNAYNLVDGVDGL
                     AGGLGSIASFTFGLWFYNYNEIPLCILSFSVFSALLAFLTYNFSPARIFMGDTGSLII
                     GLILSILAIQFIELSFNALPFSFPFRSSPAMAIAILIIPLFDTMRIFAVRILHKKSPF
                     SPDRNHMHHILQDMGCSHREICYILYAVNIGFIILALSLRNISSMILLFIILGLSIF"
     assembly_gap    2861..2888
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3099..5024
                     /gene="glgB"
                     /locus_tag="PK736_02105"
     CDS             3099..5024
                     /gene="glgB"
                     /locus_tag="PK736_02105"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRC32218.1"
                     /translation="MKKNLHVIASESLVSEFTLFTNDDIELFHAGKHCELYRLFGAHV
                     VKNDGHTGMYFAVWAPSVMQVSVMGSFNGWDKNSHKLIKRTDHSNIWEGFVAGVIEGE
                     MYKFVLTTPQGIEIEKADPFAFAAEHPPHTSSVTRDLKYKWSDAKWMKHREQKNSLQA
                     PISVYEVHLESWRRKNDDTEPMTYSELAIELVSYVKQMGFTHVEFMPVMHYPYFGSWG
                     YQITGYYCASPRFGSAQELMHLIEAFHKADIGVILDWVPSHFPGDAHGLYEFNGTHLY
                     EHPDPQRGYQPDWKSYIFNYGLHEVRSFLLSNAVFWFDKFHIDGIRVDAVASMLHRDF
                     SRNEGEWTPNVFGGNENLEAVSFIKELNEVIYKRFEGVQTIAEESTTWQGVTWPTYSG
                     GLGFGMKWMMGWMNDTLSFFKRDPLYRKHHLSQLTFSMVYAFSENFMLPLSHDEMVHG
                     KASLIYKMPGDEQQKFANLRTMYAYMYSHPGTKLLFMGGEFAQTTEWNYKESLHWELL
                     QHSPHKGMSNLIQALNKLYTTEDALYALQFDASGFEWMVANDDINTVLVYVRKSDTQK
                     IVVVLNLTPVACENYALSMPAKGSWKVILNTDDTQYGGADFCKTKSYKTSTKAADKKL
                     NVIKITLPPLSALFLKQ"
     gene            5066..5191
                     /locus_tag="PK736_02110"
     CDS             5066..5191
                     /locus_tag="PK736_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32219.1"
                     /translation="MKDIQPKHNPNNHSPDSSGKPAAAKPCVQCEDLQRIAGRTV"
     gene            complement(5255..7651)
                     /locus_tag="PK736_02115"
     CDS             complement(5255..7651)
                     /locus_tag="PK736_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRC32220.1"
                     /translation="MYIDKKSDKYSIKHYPQTITSYKRAGNFIYFISADTQMEIKVVS
                     DTIMRFRYALDEGFDRDFSYALAPDLKETLTHLEIKDTGLTYEIYTGKIICQVNKENF
                     KINIYDAHRNIILEDETGFHWQHYIWKGGKIVFCSKKIQEGESFYGLGDKPTEFNIRN
                     KRFENYGADTYGYKKDTDPLYKNIPFYMGSHHNGAYGIFFDNTFRTLFDFGAETSDVA
                     SFWARGGEMNYYFIYGPQLLNVVEQYAYLTGKPELPPLWALGYHQCRWSYFPESKVLS
                     IAHEFRKRNIPCDALYLDIDYMEGFRCFTFSKEHFPNHTKMIKTLADNGFKTVVIIDP
                     GIKVDKEYNVYSDGIKNDVYCKRSDGSLMQGDVWPGQCVFPDYTNPKAREWWSKQFDV
                     LVDAGVRGVWNDMNEPAVFEMNTFPEDVRHDFDGDPCSHRKAHNIYGHLMAKATFDGI
                     RKLLMPNRPFVITRSCYSGVQKYSSVWTGDNTATWEHLWIANMQCQRLSISGISFAGS
                     DIGGFVGEPDGELYTRWLQMAVFHPFCRTHSASNENNFDQEPWSFGHKYEFINKRYIT
                     LRYRLLPYLYSTFWQNSNEGTPMLRPLAFMDEHNKDTAHFSHQFFLGDHLMVCPVAEQ
                     GATTQPVYLPHGNWYNYWTDEIHHGHRTIIAQAPIDIIPVMVRAGAVIPKHPPMQYVG
                     EKKIDEILLHVYAGTALTRSVWYTDAGDGYGYMLGQYRISTFDVSGDKKSLTITQQID
                     GLYHAPHHRFKVVLHGTGETAKEYIVDGKSHKPAARNFAAGTLKFSCPLSFNSIEIIF
                     "
     gene            complement(8099..8998)
                     /locus_tag="PK736_02120"
     CDS             complement(8099..8998)
                     /locus_tag="PK736_02120"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HRC32221.1"
                     /translation="MKYIIILLLISGNILAQNTVCDSIMGSLTNAKFKHYNVVSSTPV
                     TTDSSQLEVVTAAWTCGGVPCNFRSLFYFDLKTFSTTTKVVSAEFTLTPKTNNFTGVP
                     GSPMYGNNNQAILSRNVKPIALNANWSNAPPWDVASYKTIPSTNTNNPFTIDVTDFVQ
                     FWIAKPDSNFGTTFKINVESHYQSMLLNSGTSNPTLQPKLEICYIKSNDTTVITNTIF
                     NYYPNPAINELNIVWTATGSRVVKYRITNVLGQEIERNSATFINGLSKIMFKHNTPPA
                     VYFIEFDYLTLKPKVIRIIKYEQ"
     gene            9505..10215
                     /locus_tag="PK736_02125"
     CDS             9505..10215
                     /locus_tag="PK736_02125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC32222.1"
                     /translation="MAHEHLYESEFDKYVEHYDEMRKYSTRAGGHSLDYYDEYKIKEI
                     ARDLGTVKCAEPINILNYGCGIGKSEPYFVKYFPNAKIVSVDVSQKSLDYAKEKLTQF
                     PQISFHYFDGHSLPCDFKFDIIFISNVFHHIEHEKHNYILTYLKSSLTKTGYIYMFEH
                     NPINPLTRKAVVDCVFDENAVLLNPKYAKSIYKNAGYTEVRIRYTLFLPAVLSSLSFV
                     ERYLNFIPIGAQYYIRAR"
     gene            10226..11371
                     /locus_tag="PK736_02130"
     CDS             10226..11371
                     /locus_tag="PK736_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32223.1"
                     /translation="MKEKDLILGMMPSRERDLNYAFIGFQQTHTSLVGIDDKGSPVFL
                     GAFDDPGIYYLFPKLASLLHCNVVLLAQITYLLIIIVATSIIWFSFKDKAKSMQQKIL
                     LYSVCGFLFALNIFKSDVYTFSYLGAAIIPFVIYLLATKKRYSYMIIFILVALSFKLN
                     FFRSHSGTISLVFFAFYFLFVIREWKTTVAFFASVVLVFIANYFITNNIAENRNKWFT
                     TNTTISIDESLSHPFWHSAYIGLGFIPNSLGLSYNDQVAMDKVYSMHPGVLFCGKIYE
                     STLKQEYLKILETETWYFVKVHLVKIFMVLGLFLFLLNIQIKKLFKLKLSQSLILPFA
                     ASILFAALSAIIVVPRINYLNAFITLCFVWFLYLMLNNTAVFSAQKK"
     gene            11462..12061
                     /locus_tag="PK736_02135"
     CDS             11462..12061
                     /locus_tag="PK736_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32224.1"
                     /translation="MKKITFLFLLLSVLSIGLFAKGQKGLARVQKLDGIEVYILSEPL
                     REYEVVTSVDKGIQAESILTGGLFNSSITDKVLQFIRKARKQGATFDAVLYSGGKSLI
                     TIKFTGNDKTNKGLCRVRTIENAEAYILCEPLEEYETIITKDGGAKWKSILTGGVINN
                     SIEEDITLLIKKMRGEGVTMDGVIYSGGKSATSIKFAKK"
     gene            complement(12396..13745)
                     /locus_tag="PK736_02140"
     CDS             complement(12396..13745)
                     /locus_tag="PK736_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRC32225.1"
                     /translation="MSRRDFVKQTTIATTAAVVMPSQFLFGNATEGKVRLAFIGTGLR
                     GQDHVELALRRTDVEIVAICDTQQLMIDNTLQLFKKAEKPLPKIFTGDNYAYRKLLEL
                     KNIDAVIISTPWEWHFTMAMDAMQAGKHVGCEVIAGATIDEMWQLVHTSEQTKMNYMM
                     LENVCYRRDVMTIMNMVRQGMFGEMVHLQGGYQHDLREVKFNDGKQPYGGGVEFGDKG
                     FSEAKWRTQHSVNRNGDLYPTHGVGPLAMMVDINRGNRFVSLVSYASKTRGLHNYIVN
                     KGDENHHNAKVRFKLGDVVTTMIKTQNEETILLQHDTSLPRPYSLGFRVQGTKGLWMD
                     VNKSLYIEGVSKDAHKWEEAKPYLEKYDHPLWKKFANDTVGAGHGGMDFFVLHQFVEA
                     IKQNKPTPMDVYDAAVWCAITPLSEQSIALGGANVDFPDFTKGQWMYRKNNFALDEE"
     gene            complement(13824..14885)
                     /locus_tag="PK736_02145"
     CDS             complement(13824..14885)
                     /locus_tag="PK736_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRC32226.1"
                     /translation="MEVSEMKNILNAYNLDASEFKCDMHGDGLINKTFLLQHTQCDEK
                     YILQKINTAVFSQPQIIANNVRNAAQYLKKNFPEYCFINFIQTKDGEDFFITYDNELW
                     RLMPFVQNSFSYNTIKNVKQAYDTAKKFGEFTARLYGAHVDLFESSIANFHNLELRYQ
                     QFTEACGNITSERYLHAKPIIDFLIYNSNIVVTYKKIVSENILPQRIIHADTKINNIL
                     FDKTTLQPVCIIDYDTIMPGYYISDIGDMIRTMVCTADENEIDKNKIVLRKDYYDAVI
                     DGYLQSMQNIMTEAEIKYIHYAGIFMTYMQALRFITDYLNNDKYYSIKYAEHNFDRAQ
                     NQCTLLRQLIELNSNRFFL"
     gene            complement(14876..15772)
                     /locus_tag="PK736_02150"
     CDS             complement(14876..15772)
                     /locus_tag="PK736_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRC32227.1"
                     /translation="MNATLLILAAGLGSRYGSTKQIDSFGPNGECIADYSIYDAMKAG
                     FTKVVFVIRQEMKPEMEKIFLTRWQNKIDIKFAIQAMDYPKHLFTEKTTRTKPWGTAH
                     AVMCAKDYINHPFIVINADDFYGADAYNNMMHFFDTNKEPHNYAMAGYKLQNTLSAFG
                     GVSRGICLADNNNMLLKITERKNIALDNKNNLVANDGLVPKNLFAKDIVSMNFWAFTP
                     TVFFEMEKMFAQFIHTHLPNDEFYIPNVVQHIIDTQTGKIKILPTNDKWFGVTYSDDK
                     QLVTDSLLHLTENKIYPSPLWK"
     gene            complement(15916..15989)
                     /locus_tag="PK736_02155"
     tRNA            complement(15916..15989)
                     /locus_tag="PK736_02155"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15953..15955),aa:Ile,seq:cat)
     gene            16111..16560
                     /locus_tag="PK736_02160"
     CDS             16111..16560
                     /locus_tag="PK736_02160"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HRC32228.1"
                     /translation="MKSIFNKEEYNSIMARIDKLTPQNQRLWGKMDVAQMLAHCVEPI
                     RAALGEITLKKNLIVMLFKGAIKKAITTEKQFTKNSPTAREYIVTDSRNFEKEKEKLK
                     SYITKFHNVGAAAFDNKEHPIVGKLTSDEWGLSVYKHLDYHLGQFGA"
     gene            16622..17347
                     /locus_tag="PK736_02165"
     CDS             16622..17347
                     /locus_tag="PK736_02165"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRC32229.1"
                     /translation="MTKLSVNINKIATLRNARGGNIPDVVQAAIDIQQFGADGITVHP
                     RPDERHIRYADVYDLKKVVFTEFNIEGYPDDKFLQLVTDVIPDQVTLVPDGPDVLTSN
                     NGWDAMANKKMLTDIVTMLHNKGMRVSLFVNPDKKMMAHAADSGADRIELYTEYYAAH
                     YDQNRQEAIDAFVETAEAATNCGLGVNAGHDLNLQNLFYFAQRIPQLLEVSIGHALIS
                     DALYYGLQNIVPMYKRCLQKPPM"
     gene            17366..18301
                     /gene="queG"
                     /locus_tag="PK736_02170"
     CDS             17366..18301
                     /gene="queG"
                     /locus_tag="PK736_02170"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRC32230.1"
                     /translation="MNKETHTKFIKQQAQDLGFMHCGISQAAFLENEAPRLEQWLQKG
                     MHGQMKYMENYFDMRIDPRKLMPGAKSVISLLLNYFPAQNITHSDDALKISKYAYGDD
                     YHHVIKSKLKLLLQQMQMHIGNIEGRAFVDSAPVLDKAWAAKSGLGWIGKNANLINPK
                     AGSFFFIAELIVDIELNYDNPMSDYCGTCNRCVEACPTQAIVAPHVVDGSKCISYFTI
                     ELKEAIPEELKGKFQNWIFGCDVCQDVCPWNRFSKPTNEKVFEPNEDLLQLSRNDYKD
                     ITEEIFTKVFSHSAIKRTKYNGLKRNIDFVKPPKN"
     gene            18303..18515
                     /locus_tag="PK736_02175"
     CDS             18303..18515
                     /locus_tag="PK736_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32231.1"
                     /translation="MLVLLSIVADKNYNNMVDLLLKKINIQHIATGCRHQMEISSLDC
                     NNEEEDFVSFMDVFLCKNEYTIYSYT"
     gene            complement(18936..20267)
                     /locus_tag="PK736_02180"
     CDS             complement(18936..20267)
                     /locus_tag="PK736_02180"
                     /inference="COORDINATES: protein motif:HMM:NF025600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HRC32232.1"
                     /translation="MIKIKTQFLLVLIILSNVSFAQLSPVITSWQQNTTSTGFAGALS
                     NVQEVNYSATYVYVKTEDIPSWIPPGYNWPNNPWFAQPMGYQFRIRRNPDPNVGPETK
                     TGYGHIGLWKNGCSIYNPKDAKHYKDSSTWFQNAWFWEHLIAETFDPCIGHPNGSGEY
                     HTHVSPACLYDITDSIQVSPLIGYAFDSHPIYGGYGYTDPMDTTSSIIRLKSSYRLRN
                     ITVRNSLPNGAILTPSLYGPPIDTNITSLNPLNNNPPGAPLGAYIEDYEYVPGLGDLD
                     EHNGRFCITKEYPNGIYAYFTTLDWVTDHYGTSIKPVFPYVIGTTYYGEVYPTDGNTG
                     PNSGFVVINEAVTPYVSTVASVGEIQEKVNIEMFPIPTSGQLNFQIDVTDFSQTYIGT
                     IYDDRGKIVEQAPVLPNKMNSYDASVLSNGVYFFKIVTNKGVYTHKFIVKK"
     gene            complement(20906..23011)
                     /locus_tag="PK736_02185"
     CDS             complement(20906..23011)
                     /locus_tag="PK736_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRC32233.1"
                     /translation="MEKIVWRKLFLIFVMMNLFLPYSYSQKGKELSAHEKKQAIIDSL
                     LILLPKAPEDTNKVKLYHNILHQHVYYNNEAGLKYEKSALELAEKLNWKIGVAGIKNV
                     AGRVYWRMGKFDDALKRHFEALYLYTEVKDEVSSAISLSFIGQDYADAGKYEQALIFF
                     KRALKKYEASGNKERIVYISYLLAWVYDNLGNYVEVSKLNYAILKQEEELGDKYRIAI
                     ALANVANDYFTLGNYPEALTYNNKAIKLLLEINEYIALSGCYINIGAIYKITANYPEA
                     IKNYSTALQLGEKIKDRFVIASACGGLGDVYQAQNNFSEALKYYNLGIENLKSIDNKQ
                     ELAELYSKSGICYARLKLYADAKRNFDKALALSKELGSNVSFTFYYQGKEILDSATGS
                     WENAYKNYKRYIVLRDSTFNKDNLTKMLSSQMQYEAEKKEAIEKAERERKEAIAKAEQ
                     EKKDIRQRTIRNSIAAVLAGSLIFLVVVYRQRNKIAKARKRSDELLLNILPAEVAEEL
                     KDKGSADAKHFDEVTVLFTDFKGFTQLSEKLTPVELVAEINECFSAFDHIMQKHGVEK
                     IKTIGDAYMAAGGIPTPNKTHAVDVVKAALDIQQFMHEHKAKREAASKLFFEIRIGIH
                     TGTVVAGIVGVKKFAYDIWGDTVNTASRMESSGEVGMINISGTTYELVKDKFNCIHRG
                     KITAKGKGEIDMYFVEEIS"
     gene            complement(23585..23887)
                     /locus_tag="PK736_02190"
     CDS             complement(23585..23887)
                     /locus_tag="PK736_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC32234.1"
                     /translation="MKTKSNITTLDQILDKKYGKKGQPKREQWEQQFEAFRLGVLLEE
                     ARTKLGLTQEELAIKCGTNKSYISRIENNSSDIRLSTLMKIIQQGLGGHLKLTLQL"
     gene            complement(23871..24215)
                     /locus_tag="PK736_02195"
     CDS             complement(23871..24215)
                     /locus_tag="PK736_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRC32235.1"
                     /translation="MTNERQVIAYKNYFLDFYEQLPDNVQTKIEWTLNLIRVTRQVPE
                     KYFKHLEGTKGLYEIRVEVGGNIYRIFSFFDKGNLVVLGNAFQKKTQKTPIQELEKAL
                     KIMEEYQNENKK"
     gene            24413..24574
                     /locus_tag="PK736_02200"
     CDS             24413..24574
                     /locus_tag="PK736_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32236.1"
                     /translation="MHHITAGCRHQMEITSLDCNIDAENVVRFIDAFVSKFNLVKLKF
                     ERGIVGVVF"
     gene            complement(24830..26257)
                     /locus_tag="PK736_02205"
     CDS             complement(24830..26257)
                     /locus_tag="PK736_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC32237.1"
                     /translation="MALNPFSAEQIIARLQFENPWWVTGSLDGYYQSFKPRLYFNLFY
                     PLVKDTTVHRASVLMGPRRVGKTVMLYHTIQQLILDGVSPQKILYASIETPIYNNIPL
                     DELFTMAKKATGDTDSNGWFIFFDEIQYLKEWEVHLKTLVDVYRKTKFIASGSAAAAL
                     KLKSNESGAGRFTDFMLPPLTFYEFIHLKNLGALIKADTINWKGNIAIFSSTTNITEL
                     NKHFINYVNFGGYPEVIFNQEIQNNPGRFMRADIIDKVLLRDLPGLYGIQDVQELNSL
                     FTTIAWNSGQECSIDELSKSSGVKKTTLRQYLSYLEAAFLVHIVKRVDQKAGRFQREN
                     FFKIYLTNPSLRSALFAPLAAESDGMGAIVETAIFSQWMQRTSFIPYYARWKNGEVDL
                     VNISRKTNRPDWAVEIKWSNRFFEAPNELKSLSSFCEENKLKQALVTSIDKSGMKEFN
                     NIQIQYLPAAVYAYNVGKNTLESQI"
     gene            complement(27209..28249)
                     /locus_tag="PK736_02210"
     CDS             complement(27209..28249)
                     /locus_tag="PK736_02210"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HRC32238.1"
                     /translation="MAQTQKRWTIAPSAGTIFAHSVDVENTKGSTPIGIQLDYSWQHT
                     DSNSFQTFSSLPFQGVSLSAFHFDNTILGNGYTLAYYLEPQLRIGKRAGIAFPAAIGL
                     SYLTNPYNPKTNAGNNSYSTHFIVYLGLSLQPYVEINKNLQLGLSASYRHVSNSGIKL
                     PNRGVNWITAELTARYFIKPQIDVQQIIKHHKQYKSKKQNRFDVCMFGASRSINSESS
                     ARYGVYGAGLLRSWQTGATHALTLGAEAYNDIGAGRRMKNDGIEGRSSVRSGLMFGHE
                     FLWGKFRFSQQVGIYVFDQTPYYSSWYHRWGLLYGLNKHISAGFVIKAHKHVAAFPDV
                     RLVYSFLKYTKS"
     gene            complement(28533..28739)
                     /locus_tag="PK736_02215"
     CDS             complement(28533..28739)
                     /locus_tag="PK736_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32239.1"
                     /translation="MTTQNFNVNAFSFEKQLSVSFNKVHKYFDDDSNNTALSIVALPF
                     DILPAGAFTKSCKETGVYQKLTDF"
     gene            complement(28932..29996)
                     /locus_tag="PK736_02220"
     CDS             complement(28932..29996)
                     /locus_tag="PK736_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRC32240.1"
                     /translation="MHREEENKIAVDKNSRSDKDIELVHSGEDYFFRLLRIISNAQSE
                     IHFQTYIFETDSTGLEIAMALKEAANRNVKVYVLLDGFGSSKLPDDFFEDLINNGVHI
                     RLFSPWHSKNNIHIGRRLHHKVVVVDSKIAIIGGINIANKYRGSSTEQPWLDYAVQVA
                     DASIAEPLGLLCKNIFLKKEHSKRKTIHSGFYKQNGTSFKILRNDWLKNLNEIYKVYI
                     NSLRKAEKEVIIVASYFLPGSRLLIVLKNAAKRGVKVKIILSGISDLPIVMRATQYLY
                     ASFLNQGIELYEWKKSVLHGKLMVIDNVWSTIGSFNLNHLSSYGSIEMNAEIKRTTFS
                     ASMLQSKLDISICSRQPSGM"
     gene            complement(29974..31062)
                     /locus_tag="PK736_02225"
     CDS             complement(29974..31062)
                     /locus_tag="PK736_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRC32241.1"
                     /translation="MKGSLKLGNIAGIGVFIHWTFTLLIFYIIFSNYRAGQSGQQLMW
                     TLLFIMSIFITVFLHELGHALAAKKYNIKTKDITLLPIGGLARLESIPEKPKEELVVA
                     FAGPAVNIVLAIITGLFITIPNTKDLSVQLSGGINSGNFFLNFFIVNLWLAIFNLIPA
                     FPMDGGRVLRALLSLKYERHIATSKAARIGQFLAIGFVFAGFFLNPFLVFIGLFIFIG
                     AQAEVEYAQAKSMLAGYKVKDVLMKEYNSIDAKETIKTAISMLLNGQSKNFLITENSN
                     SVGTLSRDEIIKALSENGENEIIHNVMNKNLIFLNADSPVEEVYHQAQQHNTTLMPVI
                     ENNKLIGVLDTENILEFIMVRDAQGRRK"
     gene            complement(31069..32718)
                     /locus_tag="PK736_02230"
     CDS             complement(31069..32718)
                     /locus_tag="PK736_02230"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HRC32242.1"
                     /translation="MQHLEIWKFLAGLGFFLYGMNQLESVLKNISGRSLKLFLRRNTQ
                     SLFKSIFGGAIITGIVQSSSVVSLIVLAFVESGIITFRNALGVILGTNLGTTLDSWIV
                     ATVGFKVNILSYSLPVIAITSIGMFFFEKKQKLYNLLTVFFALGILFLGLGFMKEGGL
                     ALVKEFDLKSFAGLSTFVFVIIGFFLTTIIQSSSATIAIALTAIYTGVLTLPSAAAVV
                     IGAEVGTTIKILLWGMKGSADKKRVAWGNFIYNIFTAVIAFISLQWLIYFIENIVQIN
                     DPLIGLVFFQTSINILSAALFIPFLNIFSKWLERKFVIDSSAGYSYISKKLPLIPVLA
                     SETLKGETKNLFVKTLLFNKNILCSEESPSEGLLENLKSFARTSASHEEEYNRLKQTE
                     GDILSYYAAIQSNLFDESETNLMLQYLDASRQSIFAAKSVKDVAHNLKEFAASSNDVK
                     HQAGKLIQDEWSAFDVFLNKLLEGDDSIEIINLIDSKLKEILLLEEHQKSEVFAKLKT
                     NQLNEVEASTLMNVYRELLSCKKSLLSALSNLKTEIVTYSK"
     gene            complement(32728..33120)
                     /locus_tag="PK736_02235"
     CDS             complement(32728..33120)
                     /locus_tag="PK736_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32243.1"
                     /translation="MEEQRLFYSNLGKLFYAIAAADKTVAEAEINTLKQIVNTEWLAL
                     DGAKDKTEVDAMRQIKITFEQLNTQKANAKNCMAEFKSFKESHAHLFTYNVKQLIWKT
                     ANAIASSFSRKNKSELVLIAELATILKQ"
     gene            complement(33157..34425)
                     /locus_tag="PK736_02240"
     CDS             complement(33157..34425)
                     /locus_tag="PK736_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRC32244.1"
                     /translation="MASLSAAVAVNIIKSGDRVFLQGSAATPSVLLKALFARKQELKG
                     VELVSITTLGEDLFTSNNIGNSFYINSLFVSKNVREIINSKHGEYVPIFLSEIPLLFK
                     NNILPIDVALIHVSPPDKHGYCSLGTSVDIVRSAIQNAKHVIAQVNKQMPRTLGDGII
                     HISEIHTIVEIDVPLPEINYSKKIDQKSLAIGKHIAGLIEDGSTLQLGIGAIPDAVLT
                     CLNNHKDLGIHTEMFSDGLIPLVKKGVITNKYKTNHQGKIVTSFVVGTKMLYDFVDDN
                     PLVSFLDVDYVNDTSIIRKNPKVVAINSAIEIDITGQVCSDSIGTYHYSGVGGQMDFM
                     RGAALSKGGKPIIAIKSATKEGYSKIVPFLKQGSGVVTTRAHVHYVVTEYGVVNLFGK
                     NLHQRSIALRDIAHPDNREELDKEIFKRFG"
     gene            complement(34523..34684)
                     /locus_tag="PK736_02245"
     CDS             complement(34523..34684)
                     /locus_tag="PK736_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32245.1"
                     /translation="MIGSLKPGNIAGVGVTIHLTHSLPIAYIVLINYLAGHTMAVDGG
                     WVARGVKAK"
     gene            complement(34734..34979)
                     /locus_tag="PK736_02250"
     CDS             complement(34734..34979)
                     /locus_tag="PK736_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HRC32246.1"
                     /translation="MNKEEIENIIFNSLKQIAPDTEPSKLLPDENIRETLGIDSFDFL
                     QFIISLDEKLNLQIPEQDYGKITTLSSLMSYLTQKMK"
     gene            complement(34976..>35075)
                     /locus_tag="PK736_02255"
     CDS             complement(34976..>35075)
                     /locus_tag="PK736_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRC32247.1"
                     /translation="ATLAADHRAADGLTGSRFLTVLNTHLQKPESL"
BASE COUNT        10712 a         6431 c         6385 g        11509 t
ORIGIN      
        1 aagaaatgaa agaaacagga ttaaaaattt tagaactgat aaaatcaaaa aaataattgt
       61 tatgacaaaa ttttcgaaaa tatttttgtt gctcgtgctt ttatcgggtt catgtttttc
      121 gcaagatttt tatttgccca ttgccaatag tttcaattta agatgggcgc cttatattaa
      181 ttctgtaaac tcaacagtac atacaggtat aaaaccctat cgcagtgcgg agctgaactc
      241 ttcttgcaat atcgattccc tcattcattt gcctgtaaat atgaaaggca ttacccaaac
      301 cagggcaggg cgcaaaatat ttaacgaggc agtggtaaaa gtaaatggcg aagattttaa
      361 tttttatcta aatcctgtgt tagatatttc gttaacacgc gaaaatactt ataagcgcaa
      421 tctgctaacg ttttcacgcg gcattcaatt acgcggaaac ataggagagc gattttcttt
      481 ttttacacaa tttaacgaaa atcaaaatga ataccctgca tatgtagata cagcaatccg
      541 atattcaaat gttgtgcccg gtctaacgcg tcctaaaggc atatatggca agcgcgattt
      601 tgccatagcc acgggtacaa taagctatag tttgaaaaaa tatttcaatt ttcaatttgg
      661 gcacgataag aattttattg gcgatggtta tcgtagcatg ttacttagcg ataattcacc
      721 caactatccc tttctgaaaa taacaaccac tatttggaaa gtgaaataca tgaacttgtt
      781 tgccgttttt caagatttaa aagtacaacc cccatcaaat tataatcccg aagattttag
      841 ctttcgtaaa aaatatggct cgtttcattt tatcgatttt aatattggca aacgtttaag
      901 cattggtatt atcgaagcag ttatctggca acatgattca attagggcta aagggttcga
      961 tataaattat attaatccat ttatattttt tcgtcctgtg gagtttagta tgggctcacc
     1021 cgataatgca atgatgggcg taaatgctaa gtttaagctc agtagccatc atattttata
     1081 cggacaattt ctactcgatg atattaaaat tgatttcctg cgcgactctt cgggctacta
     1141 cggaaataag tttgcatatc aacttgggta taaaaccttt gcgccattag gcattaaaaa
     1201 cttatcactt ctgaccgaaa taaacggagc tacaccgtat acctatcagc acaggtcgag
     1261 cattaccaat tatgcacatt acaatcaggc actggcacat ccattgggtg ccaactttat
     1321 agaatgggtt gcaatggtcg attaccattt tggacgatgt tatttcaggt tagaaaatat
     1381 aatggcaaaa acaggacaag atacaggcac aataaatttt gggggagnnn nnnnnnncta
     1441 ctacacgcct tttaaatttt tcggcaataa aacattgcaa ggtaatctta ccaaaataaa
     1501 taatcttggc atgacttttt cttattttat aaatccaagt tatgggctta atatttttgt
     1561 gaatatgaag tatcgcaaat ttgaaaacaa caaacaaagc gctaatgata tgtttatatc
     1621 ttttggcata aaaacagaat tatataattc ttattttgat ttttaagagt aattttgtga
     1681 caataagtga gtaacattaa gggtggtgcg cctgatgttt ggctataatt gggtgaaaaa
     1741 caaaaagacg aaatacgatg atgtttttta ccatacattt gaagccgttg acctattaaa
     1801 atttgcgttt aactttgctg aaggaagtta tacgcgcagc acctcttaaa tttaactatc
     1861 gatgattaat attattttcg cttctataac tgcttttatt attacttttt tggccattcc
     1921 ggctgtaata aaaattgctg atataaaagg tttatacgac actccgggag gacgcaaatt
     1981 gcacaaagca tccattcccc gattgggagg gttagccatt tttgccggtg tagcattttc
     2041 cttcgcattt tggactgccg tttttaagtt cgagcctgta caatatattt tggcctcgat
     2101 gattgtaatg ttttttatag gaataaaaga tgacattatt gaaatgcctg ccagcaaaaa
     2161 atttattggc caactaatag ccgctttaat aattgtactt tttggcgata tacgtattac
     2221 cagcatgtgc ggagtaatgg gcatttatga tattccttat tatgtgagcg ctccgttttc
     2281 tttattatta ataattctaa tagtaaatgc atacaacctt gttgatggtg ttgatggcct
     2341 ggccggtgga ttaggttcaa ttgcatcttt tacttttgga ttgtggttct acaattacaa
     2401 cgaaataccg ctttgcatac tttcattctc ggttttctcc gcattgctag catttttaac
     2461 ttacaatttt tcgcccgccc gcatttttat gggcgataca ggctcgttaa ttattggcct
     2521 catactttca attcttgcaa tacaatttat cgaattaagt ttcaatgcct tgccattttc
     2581 attcccattc cgctctagcc cggccatggc aattgcaatt ttaattattc cgctgttcga
     2641 taccatgcga atatttgccg ttcgaatact acacaaaaaa tcacctttca gtcccgatcg
     2701 taatcatatg catcacatat tacaggatat gggttgcagc catagagaga tttgttatat
     2761 cctgtatgct gtaaacattg gatttataat tcttgcttta tcgttgcgca atatcagtag
     2821 tatgatattg ttatttatca ttcttggatt gtcaatcttt nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnaa atcgaaaaca tataatatca aggtgtttaa acactagttt ttagatttaa
     2941 agtttacctg ctttcaaaaa tttttcaaaa gaattccgca aagtctcgca tcaaatagcg
     3001 agtttgaaac ttttcaactt cccctcacta aattaagtaa atcttctcat tcaattattt
     3061 gttgtaatta tgtaaccaaa ttaattacct ttcgcattat gaaaaaaaat ctccatgtta
     3121 ttgcatcaga aagtttggta agcgaattta cattatttac taatgacgat attgaattat
     3181 tccatgccgg aaagcattgc gaattgtatc ggttatttgg agcacatgtt gtaaaaaatg
     3241 atggccatac cgggatgtat tttgctgtgt gggcaccttc ggtcatgcaa gtgagtgtaa
     3301 tgggaagctt taatggatgg gataaaaatt cgcacaaact gattaaacga accgaccatt
     3361 caaatatttg ggaaggtttt gttgcaggag ttatagaagg cgaaatgtat aagtttgttc
     3421 ttactacacc ccagggaata gaaatagaaa aagccgatcc ttttgctttt gctgccgaac
     3481 acccgccaca tacatcgagt gtaactcgcg atttgaaata caaatggagc gatgcaaaat
     3541 ggatgaagca ccgcgaacaa aaaaatagtt tgcaggcacc tatatctgta tacgaagtgc
     3601 atttggaaag ttggcgcaga aaaaatgatg ataccgagcc aatgacttat tccgagttag
     3661 caattgagct tgtttcttat gtaaagcaaa tgggatttac acatgtagag tttatgcctg
     3721 taatgcatta tccatatttt gggagttggg gatatcaaat tacaggatac tattgtgcca
     3781 gcccacgctt tggaagtgcg caagaactga tgcacctgat agaagcattt cacaaagcgg
     3841 atattggcgt aattttagac tgggtgccat cacattttcc tggcgatgca catggtttgt
     3901 acgaatttaa tggcacgcat ttgtacgaac atcccgatcc acaacgtggt tatcaacccg
     3961 actggaagag ttatattttt aattatggct tgcatgaggt gcgttcgttt ttgttgagta
     4021 atgcagtgtt ttggttcgat aagtttcaca ttgatggaat acgggtagat gctgttgcat
     4081 caatgttgca ccgagatttt agccgcaacg aaggcgagtg gactccaaat gtttttggcg
     4141 gcaacgaaaa tcttgaagca gtttcgttta ttaaagaatt gaacgaagtt atctataaac
     4201 gttttgaagg agtgcaaaca attgccgaag aatcgacaac gtggcaaggt gtaacgtggc
     4261 ctacatatag cggtgggctt ggctttggta tgaaatggat gatgggatgg atgaatgaca
     4321 cgctatcgtt ttttaaacgc gatcctttgt acaggaaaca tcatttaagt caacttactt
     4381 ttagtatggt gtatgcattt agcgaaaatt ttatgttgcc attgagtcac gatgaaatgg
     4441 tgcatggcaa ggcaagcctt atttataaaa tgcctggcga tgagcaacaa aaattcgcca
     4501 acctgcgtac aatgtatgct tacatgtata gccacccggg tacaaaactt ttattcatgg
     4561 gaggagaatt tgcacaaaca acagagtgga attataaaga atcgttgcat tgggaactgc
     4621 tgcaacattc gccacataag ggtatgagca acttgataca agcattgaat aagctatata
     4681 ccacagagga tgcattgtat gcactacagt ttgatgcatc gggttttgag tggatggtag
     4741 ccaacgatga tataaatacg gtacttgtat acgttcgaaa aagcgataca caaaaaattg
     4801 tagtagtgct aaaccttaca ccggtggcat gcgaaaatta tgcgttgagc atgccggcaa
     4861 aagggtcatg gaaagtaatt ttaaacaccg atgatacaca atatggcgga gccgattttt
     4921 gcaaaacaaa aagttacaaa acatcaacaa aggctgcaga taaaaaattg aatgttataa
     4981 aaattacttt gcctccactt agtgctttgt ttttgaaaca gtagtttgtt tcccaaaaaa
     5041 atcaattcaa tactttttgt ttttaatgaa ggatatacaa ccaaagcaca accccaacaa
     5101 ccatagcccc gatagtagcg gaaagcccgc agcggccaag ccttgtgtgc agtgcgagga
     5161 cttgcagcgg atagcgggac gtaccgtttg aagaaagaag aaatgaaccg ctccaataaa
     5221 tattaataat tggagcaaca cgtttataat aaaattagaa aattatttcg atactattaa
     5281 acgataacgg gcacgaaaat ttaagtgtgc cggctgcaaa gtttcgtgct gcaggtttat
     5341 ggctttttcc atcaacaata tattcttttg cggtttcacc tgtgccatgt aacacaactt
     5401 taaatcggtg gtgtggtgcg tggtaaagtc catcaatttg ttgtgtaatg gtgagcgatt
     5461 ttttgtcgcc cgatacatcg aaagtagaaa tgcgatattg gccaagcatg tatccataac
     5521 catcaccggc atcggtatac catacgctgc gtgtaagtgc tgtgccggca taaacgtgca
     5581 gtagaatttc gtcaattttt ttctcgccta catattgcat aggagggtgc ttgggtatta
     5641 ctgcacctgc gcgcaccatt acaggtatga tgtcgatagg ggcttgggca attattgttc
     5701 ggtggccatg atgtatttca tcggtccagt agttgtacca attgccatgt ggtaaataca
     5761 cgggctgagt agtggcacct tgttcggcaa cagggcacac cataagatga tcgccaagaa
     5821 aaaactgatg actaaagtgt gctgtatctt tattgtgctc gtccataaaa gcaagcgggc
     5881 gaagcatggg cgtaccctcg ttgctatttt gccaaaatgt agaatacaaa tatggcaaca
     5941 atctgtagcg gagggtgatg tatcttttat ttatgaattc gtacttgtga ccaaaactcc
     6001 acggttcctg atcgaaatta ttttcgttac tggcgctatg tgtgcggcaa aagggatgaa
     6061 acacagccat ctgcaaccaa cgggtataga gttcgccatc gggctcacca acaaaaccgc
     6121 caatatcgct gccggcaaat gaaattcccg aaatagaaag tcgctggcat tgcatgttgg
     6181 ctatccatag gtgttcccat gttgcagtgt tatcgcctgt ccacacgctg ctatattttt
     6241 gcacaccgct gtagcacgag cgtgtaatta caaatggtcg attgggcatg agtaatttgc
     6301 gtattccatc gaaggtggcc ttggccataa gatgcccgta tatgttgtgt gctttgcggt
     6361 ggctgcaagg gtcaccatca aaatcgtggc gtacatcttc gggaaaagta ttcatttcga
     6421 aaacggcagg ttcgttcata tcgttccaca caccgcgtac accggcatca acaagtacat
     6481 caaattgttt gctccaccac tcgcgtgctt ttggattggt atagtcggga aacacacatt
     6541 ggccgggcca tacatcgcct tgcataagcg acccatcgga gcgtttgcag tacacatcgt
     6601 tctttatacc atcactgtat acgttgtatt ctttgtctac ttttataccg gggtcaatta
     6661 ttaccacggt tttaaatcca ttgtcggcaa gggtttttat catcttggta tgattgggaa
     6721 aatgttcctt gctaaaagta aaacaacgaa agccttccat gtaatcgata tcgaggtaca
     6781 acgcatcgca gggaatattg cgtttacgaa attcatgtgc tatgctaagt actttacttt
     6841 cgggaaaata gctccaccgg cactgatgat aacccaatgc ccaaagtggc ggcaactcgg
     6901 gcttgccggt taagtatgca tattgctcta ctacatttaa caattgcggg ccatagataa
     6961 agtaataatt catttcgcca ccacgcgccc aaaagcttgc tacatcgctg gtttcggcac
     7021 caaagtcgaa cagggtgcga aatgtgttat cgaaaaaaat accgtaagca ccgttgtgat
     7081 gcgagcccat atagaaagga atatttttat aaagcgggtc ggtatctttt ttataaccat
     7141 aggtatcggc cccataattt tcgaaacgct tattgcgaat attaaattcg gtaggcttat
     7201 cgcccagacc ataaaaactt tcgccttcct gaattttttt gctgcaaaaa acaattttgc
     7261 cgcccttcca aatatagtgt tgccagtgaa aaccggtttc gtcttcgagt ataatattac
     7321 gatgggcatc gtaaatattt attttgaaat tttctttgtt tacctgacaa attattttgc
     7381 cggtataaat ttcgtaggtc aggccggtat ctttaatttc gagatgtgtg agcgtttctt
     7441 taaggtcagg tgccagggca taagaaaaat cgcgatcgaa cccttcatca agcgcataac
     7501 gaaaccgcat gatggtatcg ctcaccactt ttatttccat ttgtgtatcg gccgaaataa
     7561 aataaataaa attgccggca cgcttgtaag aagttattgt ttgtggataa tgttttatgg
     7621 aatacttatc cgattttttg tcgatgtaca taaagtgatt actgtaaaaa gctttttatc
     7681 atttcggcaa atgtgaatta atatctcatt taatcaaaat tattttgtgg ttgaattaag
     7741 gcgaatgatt aataccgcca taggtgatga ttttaaaccc gcacggtttc caaaaccttg
     7801 agggtttggc ataagtactg atagttgcga ggatgatgct gcaaaaagtt ggttagttat
     7861 aacattatcc gaacgaagaa ggctttgcgg ctttaggagt tgcgtagaat ttaaaaaact
     7921 atttacatct tcacggcata aaatatattg gttatggctt ttatcgatag ggtgatgaat
     7981 ggttttttcg ttatcaaagg gttagggttg attagcttga ttgtttcgga agctattcgg
     8041 ttataaatta taacttatat acaattaata gaaacagttt attctatgta gtttcgtatc
     8101 actgctcgta ttttattata cgtatcactt ttggtttcaa tgttaagtaa tcgaactcta
     8161 taaaataaac tgcgggaggg gtattgtgct taaacataat tttagataga ccattaataa
     8221 aagttgcgct gtttcgttca atctcttgcc caagcacgtt agtaattcta tattttacaa
     8281 ctctagaacc agtagcagtc caaacaatat ttaattcgtt gatggctggg tttgggtaat
     8341 aattgaaaat tgtatttgta ataacagttg tatcgttgga tttgatataa catatttcca
     8401 acttgggttg taaagttgga tttgaagttc cactattgag taacatactt tgataatgtg
     8461 attcaacatt tattttgaat gtagttccaa aatttgaatc tggttttgca atccaaaact
     8521 gaacaaagtc tgttacatca atagtaaatg gattatttgt atttgtactc ggtattgttt
     8581 tgtaagatgc aacatcccac ggtggagcat tgctccaatt agcatttagt gcaataggtt
     8641 taacgtttct tgaaagaatg gcttggttat tattgccata cataggtgaa cccggtacac
     8701 cggtaaaatt atttgttttt ggagtcaatg tgaactctgc actcacaact tttgtggtgg
     8761 tggagaacgt ttttaaatcg aaataaaata atgaacggaa attacaaggc accccgccac
     8821 aagtccatgc agcagttaca acttccaact gtgatgaatc tgtagtcacc ggagtagaac
     8881 ttactacatt gtaatgttta aacttggcat ttgtaagact tcccattata ctatcgcaaa
     8941 cagtattttg agcaagaatg ttacctgata tcaataatag tattattata tacttcatag
     9001 ctggaatttg tttttagttg tttacaaaaa aggtgtatcc atttttttgc cccaatttaa
     9061 ggtatgcaaa tataaaaact aattatacca ttagcgtcag ttgattatta atttatatgc
     9121 agatttattc agcgctacca tttttgttct ttcaacgttg gcaccatttt taaaaaagga
     9181 tgacattata atgttcgatg aattcaatgt tcccaatcat gagtattatg ctttcaaaat
     9241 ttttacagaa agctttccta taaaattgga aatgattgct gccagattaa atttttatca
     9301 tgtggcattt aaagttttat aacaaaggac aatttcgttt ttatagcttt ataggaactg
     9361 attaacaaag cttagatatt tgtgagataa cagtcgaagt gtttgataat aacagaataa
     9421 taaatgctaa cccgataaaa aagttttttt tcttatcatt gcgcggtttc tttttatgca
     9481 tttataattt ttcaaaatcg caatatggcg cacgagcatt tatacgaatc ggaatttgat
     9541 aagtatgttg aacattatga tgagatgcgc aaatactcta cccgcgctgg tggccactcg
     9601 ctcgactatt acgatgagta taaaataaaa gaaatagcgc gggatttggg gaccgtaaaa
     9661 tgtgctgaac caatcaatat attgaactat ggttgcggca taggaaaaag tgaaccatac
     9721 tttgtaaaat atttccccaa tgcaaaaatt gtgagtgttg atgtatcgca aaaatcgctc
     9781 gattatgcca aagaaaaact tactcaattt ccccaaattt cgtttcatta tttcgatggg
     9841 cattctttgc catgcgactt taaatttgat atcattttta tttcaaatgt gtttcatcat
     9901 attgagcatg aaaagcacaa ttacattttg acctacctta aaagcagctt aacaaaaacg
     9961 ggatacattt atatgtttga acacaaccct ataaatccgc tcacccgaaa agcagttgta
    10021 gattgcgtgt ttgacgaaaa cgctgtactg cttaatccga aatatgcaaa atcaatttat
    10081 aaaaatgccg gatatacaga agttagaatc cgttacactt tgtttttgcc ggctgtatta
    10141 tcttccctta gttttgtaga aaggtatttg aattttatac caataggggc gcagtattac
    10201 attcgcgcac ggtaaaatgt aaggcgtgaa agaaaaagat ttgatattgg gaatgatgcc
    10261 atcgcgcgaa cgcgatttga attatgcatt tatcggcttt caacaaacgc atacatcgct
    10321 tgttggtatt gatgataaag gcagtcctgt ttttttgggg gcatttgatg atccgggcat
    10381 ttactacttg tttccaaaac tggcatcatt gcttcattgc aatgttgttt tactcgctca
    10441 aataacttat ttactgatta tcattgttgc cacatcaatt atttggttta gttttaaaga
    10501 taaggcgaaa tcaatgcaac aaaaaatttt actatattcg gtttgtggtt ttttgtttgc
    10561 gctaaatatt tttaaatcgg acgtatatac tttcagctat ttaggtgctg ctattattcc
    10621 ttttgtaatt tatttgttgg ccacaaaaaa aagatacagt tacatgatta tttttatttt
    10681 agttgcattg tcatttaaac tcaacttttt tcgttcccat tcaggcacaa tatctttggt
    10741 gttttttgct ttttattttt tgtttgtgat tagagaatgg aaaacaacag ttgctttttt
    10801 tgcatcggtt gttttagtct ttatagctaa ttattttatt acaaacaaca ttgccgaaaa
    10861 cagaaataaa tggtttacaa ccaacacaac catctcaatt gatgaatcgt taagtcatcc
    10921 attttggcac tcagcatata ttgggttggg ctttattcct aattcattgg ggctttcata
    10981 caacgatcag gttgcaatgg acaaagttta ttccatgcat ccgggtgttt tgttttgcgg
    11041 aaagatttat gagtcaaccc ttaaacaaga gtatttgaaa attttagaaa ccgaaacctg
    11101 gtattttgta aaagttcatc ttgttaaaat atttatggtt ttaggattgt ttcttttttt
    11161 actcaacatc caaattaaaa aattatttaa acttaaactt tcacaaagtt taattctgcc
    11221 atttgcagca agtattttgt ttgcagcctt gtcggctatt attgttgtgc ctcgcataaa
    11281 ttatcttaat gcttttataa cattatgttt cgtatggttt ttatatctca tgctaaataa
    11341 tactgccgtt tttagcgccc aaaaaaaata ataactgcat ggaaaaatac atcaaactgt
    11401 gtattgcagt gcaggaggaa atcaactaca tttgccacta taaaccataa aaaatcttgt
    11461 gatgaaaaaa attactttct tatttttact attgagcgta ttgagtattg gcttgtttgc
    11521 aaaaggacaa aagggacttg cccgtgtaca aaaacttgat ggtatagaag tttatatctt
    11581 gagtgagcca ttgcgcgaat acgaagtggt gacaagcgtt gacaaaggca tacaagccga
    11641 aagcattctt accggtgggt tattcaattc gagtattacc gataaggttt tgcagtttat
    11701 acgtaaggcc cgtaagcagg gagcaacttt tgatgctgtg ctttattcgg gaggcaagtc
    11761 gcttataaca ataaaattta ccggcaacga taaaaccaac aaaggattgt gtcgggtacg
    11821 caccattgaa aatgccgaag catatatttt gtgcgaacca ctcgaagagt acgaaaccat
    11881 aattaccaaa gatgggggag caaaatggaa atccattctt accggaggtg taataaataa
    11941 ttcgattgaa gaggatataa ctctattgat aaaaaaaatg cggggcgaag gcgtaactat
    12001 ggatggcgtt atttactctg gagggaagtc ggcaacgagc ataaaatttg cgaagaaata
    12061 aattcaaata catttagcca tagcttacct ttctaaaacg aggaaaggga cgaatgaatt
    12121 ccgaataatc ttttgccact aaaaacacga aataacaaga agcatttttc ttgtcaactt
    12181 atcaatattc gaattgcatt tctttgtgcc tttgcgtctt tgtggctaat aatttttttg
    12241 ccgcgattga attccgaata aaatgcaaat actttttgcc aaagcttgcc ccgctaaata
    12301 gtggaacggg acgaatgaat gcaaaaacat ttgcggcaac aaatcgtcat ttggtattaa
    12361 ctcatggcaa caaaccctgc tttggtataa cctttttatt cctcgtcaag agcaaaatta
    12421 ttcttgcgat acatccattg cccttttgta aaatcgggaa aatctacatt ggcaccacca
    12481 agtgcaatgc tttgttcgct gagaggagta atggcgcacc acactgctgc atcataaaca
    12541 tccataggtg tgggcttgtt ttgttttatg gcttctacaa attgatgtag cacaaaaaaa
    12601 tccataccgc catgaccggc acctacagta tcattggcga attttttcca caaagggtga
    12661 tcatattttt cgagataagg ttttgcttct tcccatttat gtgcatcctt acttacacct
    12721 tcaatgtata aagatttgtt tacatccatc cataaccctt ttgtgccttg cacccgaaac
    12781 ccaagtgaat aagggcgtgg cagtgacgta tcgtgttgca ataaaattgt ttcttcgttt
    12841 tgtgttttta tcattgtggt aactacatca ccaagcttga aacgcacttt tgcattgtga
    12901 tgattttcgt cacctttatt tacaatgtaa ttgtgcaagc cccgcgtttt gctggcataa
    12961 gaaaccaacg atacaaatcg attgccgcga ttaatatcca ccatcattgc aagcgggcct
    13021 acaccgtgag ttggatacaa atcgccattg cgatttacac tgtgttgtgt gcgccatttg
    13081 gcttcgctaa aacctttatc accaaactcc acaccgccac cataaggctg cttaccatcg
    13141 ttgaatttca cctcgcgcaa atcgtgctga tatcctcctt gcaggtgcac catttcgcca
    13201 aacattcctt gccgcaccat attcattatg gtcatcacat ccctgcgata gcaaacattt
    13261 tcgagcatca tgtaattcat tttagtttgc tcgcttgtat gaaccagttg ccacatttcg
    13321 tcaatggtgg ctcctgcaat aacttcgcaa ccaacgtgct tgccggcttg catagcatcc
    13381 atcgccatag tgaaatgcca ctcccatggt gttgaaataa ttacagcatc tatatttttt
    13441 agttccagca atttgcgata agcataatta tcaccggtaa atatttttgg caaaggcttt
    13501 tctgcttttt taaaaagttg aagtgtgttg tcaatcatta attgttgtgt gtcgcaaata
    13561 gcaacaattt caacatcggt tctgcgcagt gccagttcaa catggtcttg ccctcgaagg
    13621 ccggtgccaa taaaagctaa acgcactttg ccttcggttg cattgccaaa taaaaattgt
    13681 gatggcatta ctactgcagc agtagttgca atggttgttt gttttacgaa atctctgcgt
    13741 gatatcattt tgtatatttt aagtattgtt tgatagaaat attttcacca cgaaataaat
    13801 aaatcgaagc caataaaatg cgctcataaa aaaaatctat tactgttcaa ctcaataagt
    13861 tgccgcaata acgtgcattg attttgtgca cgatcaaaat tatgttccgc atattttatt
    13921 gaataatatt tgtcgttgtt taagtagtcg gttataaaac gcagcgcctg catgtaagtc
    13981 ataaatatgc cggcatagtg aatgtatttt atttcagcct cggtcattat attttgcatc
    14041 gattgtaagt acccgtcaat tacagcatca taataatctt tgcgaagtac aattttgttt
    14101 ttgtcaattt cattttcatc ggcagtgcaa accatcgtgc gtatcatatc gccaatgtcc
    14161 gatatatagt aacccggcat tatagtatcg taatcgataa tgcaaacagg ttgcaatgta
    14221 gttttatcga aaagtatatt gttgattttt gtatcggcat gaatgatgcg ttgtggcaaa
    14281 atattttcac tcacaatttt tttatacgtt accacgatgt tactgttgta aattaaaaaa
    14341 tcgataatag gtttagcgtg taaatatctt tcgcttgtta tattgccaca tgcctcggta
    14401 aactgttgat accgcaattc aagattatgg aagttggcaa ttgacgattc gaatagatca
    14461 acatgcgcac catataacct ggcagtaaat tcgccaaatt tttttgcagt atcatatgcc
    14521 tgcttcacat ttttaattgt attgtacgaa aaactatttt gcacaaacgg catcaatctc
    14581 cataattcat tatcatatgt aataaaaaaa tcttccccat cttttgtttg aatgaaatta
    14641 atgaagcaat attccgggaa attctttttt aaatattgtg cagcattgcg aacgttgttg
    14701 gcaatgattt gaggttgtga aaaaaccgca gtatttattt tttgcaggat gtatttttca
    14761 tcgcattgag tatgttgtaa caagaaagtt ttattgataa gtccatcgcc atgcatatcg
    14821 catttgaatt ccgatgcatc aagattgtat gcattcaaaa tatttttcat ttcacttact
    14881 tccataaagg cgaaggataa attttgtttt cggttaaatg caaaagtgaa tcggtcacga
    14941 gttgtttatc gtcactatat gtaacaccaa accatttatc gttggttggc aatattttta
    15001 tttttcctgt ttgtgtatca attatatgct gcaccacatt aggaatataa aattcatcgt
    15061 ttggcaaatg tgtgtgaata aactgcgcga acattttttc catttcgaaa aatactgttg
    15121 gagtgaaagc ccaaaagttc atggatacga tgtcttttgc aaacaaattt tttggaacca
    15181 aaccatcatt ggccacaaga ttatttttgt tgtcgagggc aatatttttt cgttcagtaa
    15241 tttttagcaa catattatta ttatcggcaa ggcaaattcc gcgcgacact cctccaaatg
    15301 cgctcagggt attttgtaac ttataacctg ccatggcata attgtgtggt tctttattag
    15361 tatcgaaaaa atgcatcatg ttgttataag catctgctcc ataaaaatca tcggcattga
    15421 taacaataaa aggatggttg atgtaatctt ttgcgcacat caccgcatgt gctgttcccc
    15481 agggtttggt gcgtgtagtt ttttcggtaa ataaatgttt tggataatcc attgcctgaa
    15541 tggcaaattt tatgtcaatt ttattttgcc atcgggttaa gaatattttt tccatttcag
    15601 gtttcatttc ctgccgaatt acaaaaacca cttttgtaaa tcctgctttc atagcatcat
    15661 aaatactata gtctgcaata cactcaccgt tggggccaaa cgaatcaatc tgctttgttg
    15721 acccatagcg gctgccgagg ccggctgcaa gtataagtag agtagcattc attagtatca
    15781 aactgctttt ataagttatg gataaaggaa attaattttt gtaagaaata aaattgctgc
    15841 ctcgatgtat gtatatggat gaaattaaaa agcaaaaaaa atccaactct tgcgaattgg
    15901 attttttaaa aagtgtgggc ccacttgggc tcgaaccaag gaccacctga ttatgagtca
    15961 ggtgctctaa ccaactgagc tatgggcccg tatttttttc gaatattatt tcaaaaaaaa
    16021 tatccctctt tttttgggtt ggcaaaaata tctattactt tgtaattaca aaacaaatat
    16081 tctttaataa tcatataaaa atttcgcatt atgaaatcaa tctttaataa agaagaatac
    16141 aatagcataa tggcccgcat cgacaagctt acgcctcaaa accagcgcct gtggggcaaa
    16201 atggatgtgg cccaaatgtt ggcacattgt gttgaaccta tacgtgcggc attgggagaa
    16261 ataacattga agaaaaattt aatcgtgatg ctttttaaag gagcaataaa aaaagccatc
    16321 acaactgaaa agcagtttac gaaaaactcc cctacagccc gcgaatatat tgtgaccgat
    16381 tcaaggaact ttgagaaaga aaaagaaaaa ttgaaatcat atattactaa gtttcataat
    16441 gtaggtgcgg ctgcgttcga taacaaagaa catcccattg taggcaaact tacttccgat
    16501 gaatggggct tgagtgttta caagcattta gattatcatt tggggcagtt tggtgcataa
    16561 ccacatactt attacagcag taaaataatc gtataaaact ttgctaataa atgcataacc
    16621 aatgacaaaa ctgagcgtaa atattaataa gatagccacc ttgcgtaatg cgcggggcgg
    16681 caatatacct gatgtggtgc aggctgctat tgatatacaa caatttggtg ccgatggcat
    16741 tacagtgcat ccgcggcccg atgaaagaca catacgttat gccgatgtgt acgatttaaa
    16801 aaaagtggtg tttactgaat ttaatattga aggctaccct gacgataaat ttttacaact
    16861 tgtaactgat gtaatacccg accaggtaac gcttgtgccc gatggccccg atgtgcttac
    16921 atcgaataat ggatgggatg caatggcgaa taaaaaaatg ttgaccgata tagtaactat
    16981 gttacataac aaaggaatgc gtgttagcct gtttgtaaac cccgataaaa aaatgatggc
    17041 gcatgctgca gattccggtg ccgacagaat agaattgtat accgaatatt atgcggcaca
    17101 ttacgatcaa aacagacaag aagccatcga tgcttttgtt gaaactgccg aggctgctac
    17161 aaattgtgga ttaggagtaa atgccggcca cgatctcaac ctgcaaaacc tgttttattt
    17221 tgctcagcgc attccgcaat tgctcgaagt atctattggt catgctttga taagcgatgc
    17281 actgtactat ggcctgcaaa atattgtgcc tatgtataag cgttgcttac aaaagccacc
    17341 catgtaattt ttatttcatc catttttgaa taaggaaacc cacacaaaat ttatcaagca
    17401 acaagcccag gacttgggct ttatgcactg tggaatatcg caagctgctt ttttagaaaa
    17461 tgaagcaccg cgcctcgaac agtggctgca aaaaggcatg catggacaaa tgaaatacat
    17521 ggaaaattat ttcgacatgc gcatcgatcc gcgaaaacta atgccgggag caaaaagtgt
    17581 gatttcgctt ttattaaatt attttcctgc acaaaacata acgcatagtg atgatgcatt
    17641 gaaaatttcg aagtatgctt atggagacga ttatcatcat gtgataaaaa gcaaattgaa
    17701 attgttgctg caacaaatgc aaatgcatat tggtaatatt gaaggccgtg catttgtcga
    17761 ttcggcaccg gtgttggata aagcttgggc tgcaaaaagt gggttgggtt ggataggcaa
    17821 aaatgcaaat ctcataaatc caaaagcggg ctcttttttt tttattgccg aattgattgt
    17881 ggatatagaa ttgaattacg ataatcccat gagcgattat tgcggcacat gtaatcgttg
    17941 tgtggaagct tgccctacac aagcaattgt agcgccacat gtagttgatg gcagcaagtg
    18001 catttcgtat ttcaccatcg agttgaaaga agctataccc gaagaattaa aaggaaaatt
    18061 tcaaaactgg atttttggtt gcgatgtgtg tcaggatgtg tgtccatgga atcggttttc
    18121 gaaacctacc aatgaaaagg tatttgaacc taacgaagat ttattgcaac tatcgcgcaa
    18181 cgactataaa gatattactg aagaaatatt tactaaagta ttttcgcact ccgctattaa
    18241 gcgcacaaaa tataatgggc ttaaacgtaa tatagatttt gtgaagccgc caaaaaatta
    18301 acttgttggt gttattgagt attgttgcag ataaaaatta caacaacatg gttgacttac
    18361 ttttgaaaaa aataaatata cagcacatag ctacaggctg ccggcatcaa atggaaatat
    18421 caagtttaga ttgcaacaat gaagaagaag attttgtaag ctttatggat gtttttctgt
    18481 gtaaaaatga atacacgatt tattcttata cataagcaaa attttggatt cttaaaaatg
    18541 ccacaaatcg tatatggtca ccgtttcatg tcataatgct gaacaaaatg gctatctgaa
    18601 atagtaaaac tccaggtaag attgccattc caacttttcc ataatgccgc cactaccacc
    18661 ttcattaaaa attgacattg ttttattttt cagtccatac tcatgagcca tcatccatca
    18721 aatgacagga caatgcgggt ctgtttgtac ttgagttaca ttcatgcacc tttgctccaa
    18781 acttattaat taagtatgtt tttgttcatg taatagcagt gactaataag ttagaagcag
    18841 tattatccga ttgttagttt attaaaccaa ctgtattttg tgcactacca gaaaactaaa
    18901 taatcagcag cgaaaaaaga acattttata aatgattact tctttacaat aaatttgtgt
    18961 gtatacactc ctttattggt tactatttta aagaaataaa cgccattcga aagtacactg
    19021 gcatcgtatg aattcatttt atttggtagc acaggagctt gttctacaat tttaccccgg
    19081 tcatcataaa tagttccaat atatgtttgt gaaaaatctg taacatcaat ttggaaattg
    19141 agttgcccgg aagtaggtat agggaacatt tcaatattta ctttttcctg gatttcacct
    19201 actgatgcta ctgtgcttac atatggtgta acagcttcgt taattactac aaatccggag
    19261 ttaggtcctg tattaccatc agtaggataa acttcaccgt agtaagtagt accaattacg
    19321 tatgggaaaa ctggtttaat gctcgtaccg taatggtcag ttacccaatc taaagttgtg
    19381 aaataagcat agatgccgtt ggggtattct ttggtgatac aaaaccttcc attgtgttca
    19441 tctaaatctc ctaatcccgg cacgtattca taatcttcta tataggcgcc taaaggtgcc
    19501 cccggaggat tgttgttcaa aggatttaaa cttgttatat tagtatcaat aggaggtccg
    19561 tataaagatg gtgttaatat agctccattt ggtaacgagt tacgtactgt aatatttcgc
    19621 aggcgatagc tgctttttaa tcttataata gaagaggttg tatccatagg gtcagtataa
    19681 ccatagccac cgtatattgg atggctgtca aatgcatatc caattaaagg cgacacctga
    19741 atgctatctg taatgtcata gaggcaagca ggactaacat gtgtgtgata ttcgcccgaa
    19801 ccattcggat gccctatgca aggatcaaat gtttccgcta ttaaatgttc ccaaaaccag
    19861 gcattttgaa accaagtgga agaatcttta taatgtttgg cgtcttttgg attatatata
    19921 gaacaaccat ttttccataa tcctatatgg ccatatccag ttttagtttc tggacctaca
    19981 ttaggatcgg gatttcttcg aatacgaaat tgatacccca ttggctgtgc aaaccatgga
    20041 ttatttggcc aattgtagcc gggtggaatc catgatggaa tgtcttcagt tttaacataa
    20101 acataagtgg cagaataatt cacttcttgt acgttggata atgcaccagc aaaaccggtg
    20161 cttgtggtat tttgttgcca gctagtgata actggcgaca gttgagcgaa tgacacattg
    20221 ctgagaataa tcagcacaag caaaaattgg gtttttattt ttatcatata ttgagtgtgt
    20281 tttacgtagg actgctaaat tatcaaaagg cttaatcttg cttgaaattt attttctact
    20341 agcaatttca acatcgttcc attagtattg tatggtgtta tttagagttg cagctaacgg
    20401 cacccggtgc aaaaaccaaa caacccacag ctagacgttt atactatgtt gtaaaaataa
    20461 aaacaacaac agcatgattg acaaacctta gcaaaaaaaa taaatatgca tcacatttct
    20521 gcgggcaaag taatcaaatg gaaatatcaa gtttagattg caacatcgat actgaaaatg
    20581 ttgtacgctt tattgatgct tttgtaagca agttcaattt agtgaaattg aaatttaaat
    20641 gaggaattgt tgtgtcggta ttttaaactt caagaggcat aaatcgtata cggtcatagt
    20701 tgtgttaggg ttttggtgtg cctaattttt aaaaaaggtt ttttcacatc ctgatgattt
    20761 gcagctacaa gaagttggcg atttcggaga cgaaaactgt ctgccaccaa tgaacttgat
    20821 acgaagcatc ccgaaacatc gtgattcatt taaccaatga accgccaatt tattaaaccc
    20881 gctgttatcg gatgtgctac ccatactatg aaatttcttc aacaaaatac atatcaatct
    20941 caccttttcc tttggctgtt atttttccac ggtgaataca attgaactta tctttcacca
    21001 attcataagt tgtaccgctg atgttaatca taccgacttc gccacttgat tccattcgtg
    21061 aagctgtgtt aacggtgtca ccccaaatat cgtaagcgaa tttctttacg cctacaattc
    21121 ccgcaactac cgttcctgtg tgaatgccaa ttcttatttc aaaaaatagt ttgcttgctg
    21181 cttcccgttt tgctttatgt tcatgcataa attgctgaat atcgagagcg gcttttacta
    21241 catcaactgc atgtgtttta tttggtgttg gaattccacc tgcagccatg tatgcatcac
    21301 caattgtttt tattttttct acaccatgtt tctgcatgat gtggtcgaat gcagaaaagc
    21361 attcattaat ttcagcgacc aattctacag gggtgagttt ttctgagagt tgcgtaaaac
    21421 ctttaaagtc agtaaacaaa acggttactt catcaaagtg ttttgcatct gcgcttccct
    21481 tatcttttaa ttcttctgca acttctgcag gtaaaatgtt caagagcaat tcatcacttc
    21541 gttttcttgc ttttgcaatt ttgtttctct gacgatatac cactactaaa aatattaatg
    21601 agcctgccag tacagctgca attgagttgc gaatggtgcg ctgtcggata tctttttttt
    21661 cctgttcggc ctttgcaata gcttctttcc tttcacgttc tgctttttct attgcttcct
    21721 ttttttcggc ttcatattgc atttgtgatg acaacatttt cgttaaattg tctttattaa
    21781 aagtgctgtc tcttaaaaca atgtaacgct tataattttt ataggcgttt tcccaacttc
    21841 ctgttgcact atccagaatt tcttttcctt ggtaataaaa ggtaaacgaa acattactcc
    21901 ctagttcttt cgataaagca agtgctttat caaagtttct ttttgcatca gcatataatt
    21961 tcaatcttgc atagcaaatt ccacttttac tatacaattc tgcaagctct tgtttattat
    22021 caattgactt caagttttca attcctaggt tgtaatattt taatgcttca gaaaaattat
    22081 tttgagcctg atatacgtct cccaatcctc cacaggcgct tgcaataaca aaacggtctt
    22141 ttattttttc tcccagttgc aatgctgttg aataattttt aattgcctca gggtaattgg
    22201 cagtgatttt ataaatagct ccaatattga tgtaacaacc tgataaggct atatattcat
    22261 ttatttcaag aagtaacttt atcgctttgt tattatatgt aagtgcctcg ggataatttc
    22321 caagtgtgaa ataatcattc gcaacgttgg ccagtgcaat ggcaattcta tatttatcac
    22381 caagctcttc ctcctgcttt aaaattgcat aatttagttt agatacttct acataattgc
    22441 ccaaattatc atacacccat gcaagaagat aggaaatata tacgattctt tctttgttgc
    22501 cacttgcttc atattttttt aaagctcttt taaaaaatat cagcgcctgt tcgtattttc
    22561 ccgcatcagc atagtcctgt ccgataaatg aaaggcttat tgcagaggac acctcatcct
    22621 ttacttcggt gtataaataa agtgcctcaa aatgccgttt caaagcatca tcaaattttc
    22681 ccattctcca gtaaacccga ccggcaacat ttttaatacc ggccacgcca atcttccaat
    22741 ttagtttttc ggcaagttcc aatgccgatt tttcatactt taaccctgcc tcattattgt
    22801 aatatacatg ctgatgcaaa atattatgat acaacttcac cttattggta tcttccggtg
    22861 ctttaggtaa gaggataagc aatgaatcaa tgattgcttg ttttttttcg tgagcactta
    22921 attcctttcc tttttgagaa taggaatatg gaagaaataa attcatcata acaaaaatca
    22981 aaaacaattt ccgccatact attttttcca ttctcatagg agtctcaaat caaataacat
    23041 atttgttgcc gttaaaagtg ggaaaatatt ttcataattt cattgcggat aattaaattt
    23101 agcaagttgt ttaaacatat ccgctaacgt cagcccatga aaaaaccatt tcccaacagc
    23161 tgcacgttaa aaacatgtgg tgaaaataaa aattacaaca acaggattga cacaccttag
    23221 caaaaaagaa aaagatatgc atcacattcc tgcgggcaga gtaatcaaat ggaaatatca
    23281 agtttagatt gcaacatcga tactgaaaat gttgtacgct ttattgatgc ttttgtaagc
    23341 aagttcaatt tagtgaaatt gaaattagaa aagtaatagt tgatgtggca ttttaaattg
    23401 cagaagtgtt atgaaacgta tatggtcatt gttaggccgc ccgccacgta acgcaaaccc
    23461 catagaaaaa catcttcacg ctacttgcga aaactaccaa aggttcagtg taacatggac
    23521 ttcctggttg gcacttcgtg ccccacaaag tcctatctgt tatagacagt gatttctcca
    23581 gtcattacag ttgaagtgtt aattttaagt gcccgccaag tccttgttga attattttca
    23641 ttagtgtcga aaggcgaatg tctgaagagt tgttttcgat acgtgaaatg taagatttat
    23701 ttgtcccaca tttgattgca agttcttctt gagtcaagcc gagttttgtt cgtgcttctt
    23761 caagtaaaac tccaagtcta aacgcttcaa attgttgttc ccattgttca cgtttgggtt
    23821 gtcctttttt accatacttt ttgtcaagta tttggtctaa tgtcgtgata ttactttttg
    23881 ttttcatttt gatattcctc cattatttta agtgcttttt ctaattcttg tattggtgtc
    23941 ttttgagttt tcttttgaaa agcattccca agaacaacta aatttccttt gtcgaagaag
    24001 gagaaaattc gataaatatt acctccaact tctactcgaa tttcgtagag tcctttggtg
    24061 ccttctaagt gcttaaaata cttttctgga acttgtcgag ttacccgaat tagatttagc
    24121 gtccactcaa tttttgtttg cacgttgtca ggtaattgct cgtaaaagtc gagaaaataa
    24181 tttttataag ctattacttg tctttcgttg gtcacgatgc aaagttaact attcagacaa
    24241 ctaattccaa atttattttt gtcggattgt cttagtattt tcggtcgtca taacattctg
    24301 actaacatct gcctgtgaaa aaaccatttc ctcaacagct gcactttaac aacatgtggt
    24361 aaaaataaaa attacaacca caccgttgac atgccttagc aaaaaaataa atatgcatca
    24421 cataactgcg ggctgccggc atcaaatgga aataacaagt ttagattgca acatcgatgc
    24481 ggaaaatgtt gtacgcttta ttgatgcatt tgtaagcaag ttcaatttag tgaaattgaa
    24541 atttgaaaga ggaattgttg gcgtggtatt ttaaattaca aagaacgcta tacatcgtat
    24601 atggtcaccg ttatggaatc gtttcggccg tcaaaatttt caatagtcag gtttttcaga
    24661 ggctgatggt atgcagctac aagaagtttg cgattacgaa gtactaaact gtcagccaac
    24721 actacacttt atacgaagca caaaagttct tttgaacacg tcactactca tttcttaaac
    24781 ccattgttgg cagaatgcgt tcttgtcctt ttgtctcttt ttattaatgt catatttggc
    24841 tttctaatgt gtttttgcca acattgtatg cataaactgc tgctggtaaa tattgaattt
    24901 gaatgttatt aaattccttc attcctgatt tgtcaattga agttaccaat gcctgcttta
    24961 gtttattttc ttcacaaaaa gaagaaaggc tctttaattc atttggtgct tcaaaaaatc
    25021 tgttactcca tttaatttct accgcccaat caggtctgtt tgtttttcta ctaatattaa
    25081 ctaagtcaac ctctccattt ttccaccttg catagtatgg aataaatgaa gttcgttgca
    25141 tccattgact aaatatggca gtttctacta ttgctcccat accgtctgat tctgctgcaa
    25201 gtggtgcaaa caacgcactg cgtaagcttg gattggtgag ataaatttta aagaagtttt
    25261 ctcgttggaa gcgtcctgct ttttggtcaa cacgttttac aatatgaact aaaaaagcag
    25321 cttctaagta tgacaaatat tgtctgagtg tggttttctt aacgccacta cttttactta
    25381 attcgtctat gctacattct tgtccactgt tccatgctat ggttgtaaaa agagaattta
    25441 attcttgaac atcttgaatt ccgtatagtc ccggtaaatc gcgaagcaat actttgtcaa
    25501 tgatatctgc tctcataaat ctgccaggat tattttgtat ttcctggtta aatattactt
    25561 caggataacc accgaaattc acataattaa taaaatgttt gtttagttca gtgatgtttg
    25621 ttgttgaaga aaaaatagca atgtttcctt tccaattgat ggtatcagct ttaattaaag
    25681 cgcctaaatt tttgaggtga ataaattcat agaatgtcaa aggaggaagc ataaaatcag
    25741 taaatcgtcc tgcgccactt tcattgcttt ttagttttaa tgcagcagca gcagaaccac
    25801 ttgcaataaa ttttgttttg cggtaaacgt caaccaatgt ttttaagtga acttcccatt
    25861 cttttagata ttgaatttca tcaaaaaata tgaaccatcc atttgaatct gtgtctccag
    25921 tagctttttt cgccattgta aaaagctcgt caagtggtat gttattataa atcggtgttt
    25981 cgattgaggc gtataaaatt ttctgaggag atacaccatc taaaattaat tgttgaatgg
    26041 tgtgataaag cataacagtt tttccaacac gtcttggtcc cattaataca ctcgctctgt
    26101 gaacggttgt gtcttttacc aatgggtaaa acagattgaa atacagtcgg ggcttgaacg
    26161 actggtaata tccgtctaag ctaccagtca cccaccaggg gttttcaaat tgcagccttg
    26221 ctattatttg ttctgctgaa aaaggattta gtgccatagt ggaggcaaat atacaaaata
    26281 aggttataaa aaaaacctta tttgactaaa taatgctttt taagttgttc agagctattt
    26341 caaaaagctt aaatggtctt ttgaaagtca actcttatga tgatttttag cacaatgacc
    26401 gttacactta ctgttatgtc agccctgaaa aaaccaactt gcccgcagct gcacgttgtt
    26461 aacaacaggt aaaataaaaa ttacaaccac acagttgaca taccttagca aaaaaaataa
    26521 atatacatca catagctgag tgctgccggc atcaaatgga aatatcaagt ttagattgca
    26581 acatcggtac tgaaaatgtt gtgcgcttta ttgatgcttt tgttttcaaa ctcgatttag
    26641 tgaaattgaa atttgaaaag gaatagttgc cccggtattt taaattataa aaagagccat
    26701 taatcgtata tggtcaccgt tacgaaacag atatggccgg caaaattttc tgaaatgggt
    26761 tttttcacaa agtcacatta ttgtggttgc catgctgctt gtaactatct ttgaatagtt
    26821 cggcttcgat tttcctgagt tccttttgaa tgtgtcagcc tgtggcatta tctaatttgc
    26881 aaaatgcaaa accctcaaac cattgtgtaa gctgtgtttt gcggtattgg ttagggtttg
    26941 ccaaaaccac tttaaagtac tacagcacct gggaataggt tttgcccgaa cctgccggtg
    27001 atttgtaaat gcttagtggc attactgatt aagctcgtaa tgatttaaca acgcttaata
    27061 acaaagatgc atgaataaaa tcaaggagca tgaaagcgaa aagaaagaca caaaacgaat
    27121 tataccgaaa caacttagat tttggaaaat ttttcttttg aagttgttcg gcattactac
    27181 aagtaagcag ttctttttca aaaaactgct aacttttggt atactttaaa aaactataaa
    27241 caagccttac gtcaggaaaa gcagcaacat gtttgtgggc ctttattaca aaacccgcgc
    27301 ttatgtgctt gtttaaacca tacaataaac cccaacgatg ataccagcta ctgtaataag
    27361 gtgtttggtc aaatacgtat ataccaactt gctgactgaa tctaaattta ccccataaaa
    27421 actcatggcc aaacatcagt ccacttctta ctgaactacg gccttctatt ccatcgtttt
    27481 tcattcgcct gccggcacca atatcgttgt aagcctcagc gcccagggtt agtgcatgtg
    27541 ttgcaccagt ttgccatgaa cgcaataacc ctgcaccata taccccatat ctggcagatg
    27601 actcactgtt aatgctacgt gatgcaccaa acatgcacac atcaaaacgg ttttgttttt
    27661 tacttttata ctgtttatgg tgctttataa tttgttgcac atcaatttga ggtttgataa
    27721 agtaccgggc cgttaattca gcagtaatcc agttaacacc cctgttaggc aactttatgc
    27781 cactattaga tacatgcctg taacttgcgc ttaatcctaa ttgaagattt ttatttatct
    27841 ccacataagg ctgtaagctt aatcccagat aaacaataaa gtgtgtgctg taagaattat
    27901 taccggcatt agttttaggg ttgtaaggat tggtaaggta gctaagacct attgctgcag
    27961 gaaatgcaat gcctgctctt ttgccaatgc gtagctgagg ttctaaatag taagccagcg
    28021 tataaccatt tcctaatatg gtattatcaa aatggaatgc actaaggctg acgccctgaa
    28081 aaggcaagct tgaaaaagtt tggaagctgt tgctgtcggt atgctgccag gaataatcca
    28141 actgaatacc tatgggagtt gagcctttgg tattttctac atccacagag tgggcaaaaa
    28201 tagttcctgc gctgggcgct atggtccaac gcttttgtgt ttgtgccatt gcaaaaacat
    28261 agagtataat gcatggcaac aggagtacaa attttcttat attaattgcc cgttgcatca
    28321 agtttatcac ctgttaatgt tactttccat ataattaaaa aagataatga gcggtacgct
    28381 tatatcctca ggatgctcgc attcaattta taatttctcc ggctaatgta tcatttttgc
    28441 tcagcttgtt tttattatac cgaaacaact taggtttggg aaaatttttc ttttgaagtt
    28501 gttcggcatt accataagta aacagttttt tttcaaaaat ctgttaactt ttggtatact
    28561 ccggtttctt tgcacgattt tgtaaaagcc ccggcaggca gtatatcaaa tggaagggca
    28621 acaatgctta acgcagtatt atttgaatca tcatcaaaat atttatgtac tttgttaaag
    28681 gatacgctta attgcttttc aaatgaaaaa gcatttacat tgaagttttg tgttgtcatg
    28741 ttctttttta aatttaaatt ttttttgttg tccggtctcg tggcattggc tataacgtca
    28801 gcctcactgc ctaaggcgcg acaagttgta aaaaaaccaa acagtccaca gttgcacatt
    28861 aacaacattt ggtaaaaata aaaattacaa ccacaccgtt gacatacctt agcaaaaaaa
    28921 ataaatatgc attacatacc tgagggctgc cggctgcaaa tagaaatatc aagtttagat
    28981 tgcaacatcg atgcggaaaa tgttgtacgc tttatttctg cattcatttc aatgctccca
    29041 taagaactta aatggtttag attgaatgac ccaatggttg accacacatt atcgataacc
    29101 attaatttcc catgtaaaac agattttttc cactcatata attcaatccc ctgattaaga
    29161 aacgatgcat aaagatactg agtggctcgc attacaattg gcaaatcgga aattcctgag
    29221 agaatgattt taacttttac tcctcttttt gccgcatttt ttaatactat taataatcta
    29281 ctaccgggca aaaaataact tgccacaata atcacttcct tttccgcttt tcttaaagaa
    29341 ttaatataga ccttataaat ttcgttcaaa tttttaagcc agtcgttacg caatatttta
    29401 aacgatgtgc cattctgttt gtaaaaacct gagtgtatgg tttttctttt agagtgttcc
    29461 ttcttcagaa aaatattttt gcataacagt ccgagaggtt cagcaataga tgcatctgca
    29521 acctgcacag cataatcaag ccagggttgt tccgtacttg aaccgcgata cttatttgca
    29581 atgttgatac cgccaataat ggctatttta ctatcaacca caactacttt atgatgcaac
    29641 cttcttccaa tatgaatatt gtttttggag tgccatggag agaataaacg gatgtgtaca
    29701 ccattattta ttaagtcttc gaaaaagtca tctggtaatt ttgaagaacc aaaaccgtca
    29761 agtaaaacat acactttaac attgcgattc gcggcttcct ttaacgccat tgcaatttct
    29821 aaaccggttg aatcggtttc aaatatgtag gtttgaaaat gtatttcaga ttgcgcattg
    29881 gaaataatac gcagtaaccg aaaaaaataa tcctcgccac tatgaaccaa ttcaatatct
    29941 ttatccgacc tgctgttttt atcaacggct attttatttt cttcttccct gtgcatctct
    30001 taccatgata aattctaaaa tgttttcagt atccaataca ccaataagtt tattgttttc
    30061 aattacaggc attagtgtag tattatgctg ttgtgcttga tgataaactt cttcgacagg
    30121 agaatcggca ttaagaaaaa ttaagttttt gttcatcaca ttatgtatga tctcattttc
    30181 tccattttcg gataatgcct ttataatttc atcacggctc aaggtgccca ccgaattaga
    30241 attttcggtg ataagaaaat ttttactctg tccgttaaga agcatgctta ttgctgtttt
    30301 gattgtttct tttgcatcta tcgagttgta ttctttcatc aatacatctt taactttata
    30361 gccggcaagc attgattttg cttgtgcgta ttcaacttct gcctgtgcac caataaaaat
    30421 aaacagccca ataaaaacta aaaacgggtt cagaaaaaat cctgcaaaca caaacccaat
    30481 tgcaagaaat tgacctatgc gcgctgcctt gcttgttgca atatgccttt catatttcag
    30541 ggaaagcaaa gctcttaata ctcttccccc atccataggg aaagccggaa ttaaattgaa
    30601 tatggccaac cacaaattga caataaaaaa attaaggaaa aaatttccgc tgtttattcc
    30661 acctgataat tgaacgctta aatcttttgt attcggtatg gtaataaaca aacctgttat
    30721 aattgctaaa acaatattta cagcaggacc tgcaaaagca accacaagtt cttctttggg
    30781 tttttcggga atagattcca atcgggcaag gcctccaata ggcagaagtg taatatcttt
    30841 tgttttaatg ttgtattttt ttgctgccag ggcatgtcct aattcgtgca ggaaaacagt
    30901 aatgaaaatg ctcataatga aaagcaatgt ccacattaat tgttgcccgc tctgccctgc
    30961 cctgtagtta ctgaaaataa tataaaatat caataacgta aatgtccagt gtataaatac
    31021 acctatgcct gcaatgtttc caagtttgag tgatcctttc ataatattct attttgagta
    31081 agttactatt tccgttttta aattacttaa tgccgacaat agagatttct tgcatgataa
    31141 caattcgcga tatacattca taagtgtaga tgcttctact tcgttcagtt gattggtttt
    31201 gagttttgca aacacttcag atttctgatg ttcttccaac aataaaattt cttttagttt
    31261 actgtcaatt aaatttataa tctcgatgct gtcatcaccc tccagcaatt tatttaaaaa
    31321 cacatcaaat gctgaccatt catcctgtat tagtttaccc gcctgatgtt ttacatcatt
    31381 tgatgatgcg gcaaattctt tcaggttatg cgccacatct tttaccgact ttgctgcaaa
    31441 aatagattgt cttgatgcat ctaaatattg caacattaaa tttgtttcac tctcatcaaa
    31501 aaggtttgat tgaatagctg cataataact caagatgtct ccttctgttt gctttaagcg
    31561 attgtattct tcttcatgag aagcggaagt tcgcgcaaag gactttaagt tttccaataa
    31621 accctctgac ggagattctt cggaacacaa aatatttttg ttgaacagca aagtttttac
    31681 aaacaggttt ttcgtttcac ctttcaatgt ttcggaagca agcaccggta tcaagggcaa
    31741 ttttttactt atgtatgaat agccggcaga gctgtcaata acaaattttc gttccagcca
    31801 ttttgaaaaa atattcagaa acggaataaa taaggctgcc gataatatgt tgattgatgt
    31861 ttgaaaaaac accaatccaa ttaaggggtc gtttatctga acaatatttt caataaaata
    31921 aataagccat tgtaaggaga taaatgcgat gacggctgtg aagatattat aaatgaaatt
    31981 tccccatgca accctttttt tgtcggcaga acctttcatt ccccacaaaa gtattttaat
    32041 ggtggtgcct acttcagcac caattacaac tgctgcggct gacggtaatg ttaaaacacc
    32101 ggtgtaaatt gctgtaaggg caatggctat agttgccgaa ctggattgaa taattgtagt
    32161 gagaaaaaag ccgatgatta caaaaacaaa agtgcttaaa ccggcaaatg atttcaaatc
    32221 aaattctttc accaatgcta atcctccttc tttcataaag cccaatccta aaaacaaaat
    32281 accgagtgcg aaaaaaacag tcagcaggtt gtacagtttc tgcttctttt caaaaaaaaa
    32341 cattccaatt gaagtaattg caatcacagg aagcgaataa cttaaaatgt tcaccttaaa
    32401 ccccacagtt gcaacaatcc aactgtctaa tgttgtgcct aagtttgttc ctaaaattac
    32461 gccaagcgca ttgcgaaaag taataatgcc cgattctaca aaagccaaaa caatcaatga
    32521 tactaccgag ctgctttgta caatgcctgt tatgattgct ccaccgaaaa tggatttgaa
    32581 taaactttga gtgtttcgtc taagaaatag ttttagcgat cgccccgaaa tatttttcag
    32641 cacgctttcg agttgattca ttccgtaaag aaaaaaacct agtccggcca aaaatttcca
    32701 tatttctaag tgctgcatct acttcactta ttgttttaat attgttgcca gttcagcaat
    32761 aagcactaat tcggatttgt tttttcttga gaaagatgag gctattgcat ttgccgtttt
    32821 ccaaatcaat tgctttacat tataggtgaa caagtgcgca tgcgattctt taaatgactt
    32881 aaattctgcc atgcaatttt tggcattagc tttttgtgta ttgagttgtt caaaagtgat
    32941 ttttatttgt cgcatagcat ccacctctgt tttatcttta gcgccatcta aagccagcca
    33001 ctcagtattt acaatttgtt ttaatgtgtt tatttctgcc tcagctacag ttttatcggc
    33061 agcagctata gcataaaaca atttaccaag attgctgtaa aataatcttt gttcttccat
    33121 ttatgatttc atttactcat atcgccacta acttttttag ccaaaccgtt taaatatttc
    33181 cttatccaat tcttctctat tgtcgggatg tgcaatatct cttaatgcaa ttgatctttg
    33241 gtgtaaattt tttccgaaca aattaactac accgtattct gtaacaacat aatgtacatg
    33301 tgcgcgagtg gttaccacac cggaaccttg ttttaaaaag gggactattt tcgaatatcc
    33361 ttctttagtg gctgatttaa ttgctatgat tggtttcccg cctttggaca aagccgcacc
    33421 tctcataaaa tccatttgtc cgcccacacc ggagtagtga taggtgccaa tagaatcgga
    33481 acaaacctgt ccggtgatgt caatttcaat agcagaattt attgccacta ctttcggatt
    33541 tttccgtatt atacttgtgt cgtttacata atcaacatct aaaaaagaaa ccagtggatt
    33601 gtcgtccaca aagtcataaa gcattttagt acccacaaca aagcttgtta caattttccc
    33661 ctgatggttg gttttatatt tatttgtaat cacacctttt ttcacaagcg gaatcaaacc
    33721 atcggaaaac atttcggtat gtatgcctaa atctttatga ttgttgaggc aagttaatac
    33781 agcgtccgga atagcgccaa taccaagttg aagagttgag ccatcttcaa ttaaaccggc
    33841 tatatgtttt cctatggcta acgatttttg gtcaatcttt ttgctgtaat ttatttccgg
    33901 caaaggcaca tcaatttcta caattgtatg aatctctgaa atatgtataa tgccatcacc
    33961 aagtgtgcga ggcatttgtt tatttacctg tgcaataacg tgcttagcat tttgaatagc
    34021 cgaacgcaca atatcaaccg aggttcctaa agagcaatat ccatgtttgt cgggaggaga
    34081 aacatgtatt aatgctacat cgattggtaa aatattgttt ttaaataaca gaggtatttc
    34141 actcagaaat attggcacat actctccgtg tttcgaatta attatttcac gtacattttt
    34201 tgaaacaaaa agagagttga tataaaagct gttaccaata ttgttggagg tgaaaaggtc
    34261 ttctcccaaa gtggtaatac ttaccagttc aactcctttg agttcttgct tacgtgcaaa
    34321 caatgccttt aacaaaacag atggtgtagc ggcactgcct tgtaagaata cacggtcacc
    34381 acttttaatt atattaactg caaccgctgc tgataaagaa gccataaata gaacaattta
    34441 atttacaaca ttttcataat tgcaatgttt acacaatgca cctaaattag caatggtggt
    34501 atttgcaatt cgtgataaag cttcattttg cttttactcc ccgtgcaacc cagccgccat
    34561 caaccgccat tgtgtgccct gcaagataat tgataagcac gatataagca atgggcaatg
    34621 aatgtgtcaa gtgtatagtt acgccaacgc ctgcaatgtt tccgggtttt aatgagccaa
    34681 tcataattat ggtttcgttt tagtcgttga atctattttt tttactaagc gaatcatttc
    34741 attttttgtg tcaaataact catcaacgaa ctcaatgtgg taatctttcc ataatcctgt
    34801 tcggggattt gtaggtttag tttttcatcc aatgagataa taaactgcaa aaaatcgaaa
    34861 gaatctatcc ccagtgtttc tcttatgttt tcatcgggca ataatttcga tggctcggta
    34921 tcaggtgcta tctgcttcaa agagttaaaa attatatttt caatttcttc tttattcata
    34981 atgattcagg tttttgtaaa tgagtattta atactgttaa aaatcggctg cctgtgagcc
    35041 cgtcagctgc acgatggtct gcagcaagtg tagca
//
LOCUS       DAORQA010000013        29890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_359_length_29890_cov_22.611061, whole genome shotgun sequence.
ACCESSION   DAORQA010000013 DAORQA010000000
VERSION     DAORQA010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 29890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29890
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_359_length_29890_cov_22.611061"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PK736_02260"
     CDS             complement(<1..645)
                     /locus_tag="PK736_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32248.1"
                     /translation="MKNNFKISIFIMAIFAVMNSTICKAQYIQSLNVSPPYPTTNDSI
                     TINAMCQFPFGGCDIKYQQQFVNGNNLYGQAMHCLGMLTVICDETDVFKFAPLPAGTY
                     TFNFQLDATQAMPPCTPGIVPGPTDTISFVVINANSLPENSNLAINIFYTDNQLHLFS
                     NTNEYNNQLLKIEVLDITGKILYNENLMLNNNAANIRTNIHNQLVIVKVSDSKGN"
     gene            819..2351
                     /locus_tag="PK736_02265"
     CDS             819..2351
                     /locus_tag="PK736_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32249.1"
                     /translation="MKKSLLTLLISIAFLQSKAQVCQIDCFEQTDSISQNVTNLLLNG
                     SFESTTCAPFSVGWNVYCPNSAAYNCDVMNWTCTGGGASSYPLIFSAASSIIADGLYG
                     AYMGNAFSENCGAVAGDTSCLVDNNCEVTGLPPGFPNNGSAYGGNTGVSIEQTVTGLI
                     PGNYYVLEFWTGGEGFNSYPNRGLFGLDVGFGYNYLRCKGTTALSTDVGIRYVVQFLA
                     NSTSHTIKFTNWGHICSSCTEVMVDHVRLYTQAQLSPLISPCGGSGAAPVVNIASSDT
                     IWCDKQAIDFTDLSTNMPTSWMWYFTGATPSTSTDQNPVGIYYPSQGAFSVTLVACNA
                     IGCDSVTFVNFVQEFPSPVPPVVSVINDSLCVSGYVTYQWFETGNLTNVLSTNSCFVP
                     TVAGNYFALVTDTNGCEVASGTVPVTVSIYEQDALSKIKIIPNPFSDITSLEFNIDDV
                     QTTAVAIVDVTGREVMTVDSKNFQPGKNKLQLNLSLVNDGIYFCKIGSQSKVMMVKLV
                     KR"
     gene            complement(2668..6114)
                     /locus_tag="PK736_02270"
     CDS             complement(2668..6114)
                     /locus_tag="PK736_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRC32250.1"
                     /translation="MKNNFSFHILVMFIAFSNSLFANSDTLIHKNATWKYYDHVNTTI
                     PNWKLLTYNDSLWASGAGQFGYGDGDEATVIYYGVSAKTKYIAYYFRKKINIANPAIY
                     DSLRINMLCDDGAVIYLNGIEVKRTNLPLTTIGIKTRALTSINPPTESQYFPYIISNT
                     NLIAGENIIAVEVHQYSSGNSDLSFDMQLLGFYSLCNAPLNTNATNITTYSSLISWNS
                     APYAVTYQLDYKKQTDTTWTSINAINDTSITIANLDYNTTYNYRVKTNCAAYSSSFTT
                     INNFTTLTPVCQSPVNLNATNILTASGSVSWSTVQDAINYKLIYKISSASAWDTITSN
                     DTSFLLNSLLPSSTYNWKVIAICAYGNSNESNTANFTTLTPCPATINNIASNITSNAA
                     TLKWKTTTGTGQYEVEYRGMNGQWITVTVNDTFLVLNSLVSATTYSWHVHALCAPGNT
                     GAWSDYNSFTTSQVYFSTDTLIAKNATWKYLDNGSNQNTAWQLPSFVDAGWANGSAEL
                     GYGDGDEATIVSYGPSSSNKFITTYFRKTFSVTNPSQYDSIQIGLVRDDGAVVYVNGV
                     EVIRTNMPVTAINYLTLSPVAIAGTDESAYNIFYVSKSFFTSGSNCIAVEVHQQLASS
                     SDISFNMFVAGVVGSPTINLVRGPYLQVLTPNSVNIRWASDISTNSKVYYGTDLSYGS
                     AVDSATITTEHEIKLTGLLPNTKYYYTVGSSLKNIQGDDKNYFTTSLTTGSNASFTVW
                     ATGDFGKITTGQAGVKSAYLNYGKRANFWIWLGDNAYESGTTAEYNSTVFNQYPDIFK
                     KTPVYPAPGNHDYANAGYLSVSALGTNFPYFNFFTVPTQAQAGGVASNSKKYYSYDFG
                     NTHFISLDSYGALNNNTSAMYTWLQNDLLNNTSKFKVAYFHHPPYSMGTHNSDTETAM
                     IDIRTNICTLLESYDVDLVLSGHSHTYERSILMHGNFGLENSISPAMKIDTSSGNNPF
                     YSKSLPGGTGTVYAVCGISGKGGPGQTVQGSWPHAAMYSYSQSLYGSLILEINGDTLS
                     SKLLTSTGSVFDQFKIVKSNGARQTTYFNNEEENDLTIFPNPFGDDLTVNIDASNDEN
                     VKIELMDITGRTIPFTEQRTIDHHQIISTISLPKNTPSGVILIKIINGSKIQYTNVYY
                     IR"
     gene            complement(6233..6760)
                     /locus_tag="PK736_02275"
     CDS             complement(6233..6760)
                     /locus_tag="PK736_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC32251.1"
                     /translation="MKIITTTRLYLREKTADDAENAYLLNLDPDVIKYTGDPPFESIE
                     AARIFLQNYNHYTKYGFGRWAVIDKATDEYLGWCGLKFSADLNEYDIGFRFFKRHWNK
                     GYATEAAQACLKLGFENFKMEKIVGRAMKLNSGSIRVLQKLGMTYIGTFDFDKHKGEL
                     YSLTLNDYLTQTPVQ"
     gene            complement(6824..7570)
                     /locus_tag="PK736_02280"
     CDS             complement(6824..7570)
                     /locus_tag="PK736_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRC32252.1"
                     /translation="MLKNIASTIYSIYALIVFIPISLVVFILYVIVFGIIPQPKSSRI
                     AHRISQVWAHVTYTVLFIRVKIKNKHLIPKEEGVVFISNHRSLLDVPLWAISTPHVVK
                     YLSKEEMTKVPVFGYAIKRMYITVNRADSKDRHLSIIKMVNALKAGTSIYLAPEGTRN
                     KTKEILLPFKDGAFRMAIAAQAPICVFTLYNTDKLLNPKKLLSLLPGTIRGTWAEPIY
                     TKGMTENDIPALREKVRNIMETNYVMMSKL"
     gene            complement(7647..7991)
                     /locus_tag="PK736_02285"
     CDS             complement(7647..7991)
                     /locus_tag="PK736_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HRC32253.1"
                     /translation="MDKYLKFLLLFIIFTSAFSNVSNAQTDTAVIATSAQCDMCKNKI
                     ENALRFEKGVKLATLDVKSKNVTVIYNSKKTDADKIRKAISMLGYDADNISADSASYN
                     KLHECCRKPHLE"
     gene            complement(8024..10246)
                     /locus_tag="PK736_02290"
     CDS             complement(8024..10246)
                     /locus_tag="PK736_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC32254.1"
                     /translation="MSKYISIIFIFVCAQLNAQSIIRGQVLVNDAEMGYKPLPFASVY
                     SEDKLLSTLTDSMGFFQLESKTLPIRIVVSMVGYTNDTVTGNNTDMILRVVLKPGKQL
                     KEIVVQGKQEGLKISTLQPINTEVITSKELLRAACCNLSESFETNPSVSINYTDAITG
                     AKEIQLLGLSGIYTQLLNENISSMNGLAQAYGLGYVPGPWMESIQINKGAGSVVNGYD
                     ALAGSINIEYIKPEEAAEKAYINLFGSQFGETETNFYTIHKLNEHLSTMMMLHTEYFS
                     QKRDHNDDGFLDMPLKQQVNFYNRWRYHSGKKLEGQFGIKALAELREGGQKEYFTNEN
                     KDKLFFGSQIKTNRVEMYTKTGMVFPEHPNKSLGLQMNYVYHKMNSFFGLKKYDAIQN
                     SYNANLIYMNELWNCNNKIKAGASFRYMNIDGVYQHISDNFSIGNYEIVPGIFAEYNY
                     KPNEQFAVIAGGRADYHNEFGMFYTGRMNAKYNFTENFIVRVAGGNSFRTANIIADNI
                     GLMADGKRLLPLEDLLPERAWNYGINTTYKFLIAHREGSLNIDAYRTEFVNQVIIDPY
                     TDRDFIQLYNLKGESYAQSFSVAINYNLIPQLDMRLAYKLDDVHITYFETEIEKPLIG
                     RHKGLFNLGYESINKKWRVDYTIQIDGSKKIALQSEGHHGGSTFQTSPIFTLMNGQVT
                     KVFRKFELYAGVENATNFTQHHPIINAENPFSREFDATQIWGPIMGRRIYAGFRMKII
                     "
     gene            10844..12136
                     /gene="eno"
                     /locus_tag="PK736_02295"
     CDS             10844..12136
                     /gene="eno"
                     /locus_tag="PK736_02295"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRC32255.1"
                     /translation="MSNVLAINAREILDSRGNPTVEVDVTTTNGIIGRAAVPSGASTG
                     KHEAAELRDNDKSRYMGKGVTRAVDNVNGPILEELRGLSVFAQHDIDHLMMQLDGTPN
                     KSSLGANAILGVSLAIAHAASQELGLPLFRYVGGVCANELPIPMMNILNGGSHADNSI
                     DFQEFMIMPTGADSFAEALRMGAEIFHNLKKVLLDKKLSTNVGDEGGFAPNLKSNEEG
                     IQMVMKAIEAAGYKPGENVFIAMDAAVTEFYIEEEKMYHFHKSTGDKLSSSDMVSFWK
                     DWVYKYPIISIEDGLAEDDWDGWKELTAALGNKIQLVGDDLFVTNVNRLQQGIDKGVA
                     NSILVKVNQIGSLTETINTVQLAKNNNYTTVMSHRSGETEDTTIADLAVALNCGQIKT
                     GSLSRSDRISKYNQLLRIEEVLGNSATYKGRGFKFAKR"
     gene            12184..12585
                     /locus_tag="PK736_02300"
     CDS             12184..12585
                     /locus_tag="PK736_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32256.1"
                     /translation="MADENSKVNLPDNEDEFEEQEIEQEIEVDGEPKLDNDGNKEFPD
                     WVSGQRTSSSGRKRVKKRIKIKKKIKVRKKSSTRKYKKLIERLIWILVILGFIAALVV
                     LVNELDINDAKYKKKKSFRSESVFQKDIIRT"
     gene            12619..13941
                     /locus_tag="PK736_02305"
     CDS             12619..13941
                     /locus_tag="PK736_02305"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase, eukaryotic"
                     /protein_id="HRC32257.1"
                     /translation="MDRIKEKFAQKAIPMAAEIKELVKQHGNFKLGEYTIEQVYSGMK
                     GMIGMITETSKLDPEEGIRFRGYSIPELREKLPHSDEGPEPLPEGMFYLLLTGDLPTN
                     QDVKEINNEWMKRSTVPKHVFDMLDMLPAKTHPMTQFSMAIMALQTESVFAQRYRDGM
                     NKKEYWDAMFEDILTLIARLPRIAAYIYRNTYKNGVHIEPNPKLDWAANFAHMLGFDT
                     PGMRKLMRLYLTIHVDHEGGNVSAHATHLVGSALSDAYLSLAAGMNGLAGPLHGLAAQ
                     EVARWVIELREKFNGVPTKEQIEEYIKQTLSEGRVVPGYGHAVLRKTDPRYTAQQDFA
                     KENMANDELFKIVQMLYEVTPEILKATGKIKNPWPNVDAHSGILLMHYGLNEYDFYTV
                     LFGVSRAIGVLTSLLWDRALGSPIERPGSVTTEWVKKKIAEQAAVTPS"
     gene            14393..15625
                     /locus_tag="PK736_02310"
     CDS             14393..15625
                     /locus_tag="PK736_02310"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32258.1"
                     /translation="MKKNLLIIILLFCSFNECKAQWTKINLPNNWSIAQMQFVNDSVG
                     YMIADSIVGLSLAGKVYKTFDRGLNWYEKSFYYQSFDYLYFINEDTGWICMTDGGTGT
                     IRRTVNGGSTWTNICFNAPHGPMFFVTPLVGYIGWIAAGAQGAVLYKTIDGGITWNSV
                     GVNLGLSGLQDMVFADSVTGYVGGLYGPTLGRLDLTVPSYNVFSTSIAVSDMDYENAS
                     AQLFFTGEEFGVNSGLYKTTSNGSPINLILDAQSGFGAYSVNCKNANEIMCGGYAGLI
                     AFSNDGGINWNTESTGSQTSFTDVHWGSNYAIAAGGDGEVYRKSLPLSFIENSAVDDF
                     AIYPNPAENIINIKTKIEGELTVTIYDVLQKKVLEQKITPCEFCTVDVSALQKAFYTV
                     VLSNAKKEVIGKQKLLLK"
     gene            complement(15719..16177)
                     /locus_tag="PK736_02315"
     CDS             complement(15719..16177)
                     /locus_tag="PK736_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HRC32259.1"
                     /translation="MFESLLNLVKENAGDAIINNPAIPNEHNDAAIETATSGIMSGLK
                     GAVGGGNLEGIMSMFSGGSANAGSNPIVNAISGQVAGNLMEKFGINNAAAGSIVQSLI
                     PTVMSKLVHKTNDANDSSFDIGGIVSSLTGGNQAGGIGGMIGNITKMFGK"
     gene            complement(16223..16987)
                     /locus_tag="PK736_02320"
     CDS             complement(16223..16987)
                     /locus_tag="PK736_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003007682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC32260.1"
                     /translation="MKVTFLGTGTSQGVPVISCPCKICTSSNPRDNRLRSAILIEDEG
                     TNIVIDTGPDFRQQMLRSQVKHLDAIVFTHEHKDHLAGLDDIRAFNYFNKKRVDVYAH
                     ERVQAAIKREFPYIFDGNNYPGIPEIDLYDIRAGVNIQINNITLQPLEVMHYQLPVFG
                     FRINNFVYITDANFIAPHVMREIKGVDHFVINALRREKHISHFTLDEALAVIEEVNPG
                     HAYLTHISHQLGLHDDVAAELPKNVVPAYDGMIIEM"
     gene            complement(17009..17305)
                     /locus_tag="PK736_02325"
     CDS             complement(17009..17305)
                     /locus_tag="PK736_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32261.1"
                     /translation="MKYTQNFLKKVEDQLNESGYQVRYEKGNFKSGYCVLETKKVVVV
                     NKFVTLENKINTLVEILNLLGNQSMENNAMLNNSENADNTEDTSLEKSEMQSAN"
     gene            complement(17437..18039)
                     /locus_tag="PK736_02330"
     CDS             complement(17437..18039)
                     /locus_tag="PK736_02330"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HRC32262.1"
                     /translation="MKKIFFIVLLFTMQVTCIAQPQKLPAIIINDGDTMPVVYMRAIE
                     VVGNLNPEAAANLAKYNKLRRDVLRAYPYAKIAAAKLKEINDHAATLTRERDRKEYIK
                     STEKAMKEQFEGDLKKLTIGQGRILIKLIDRQTGNTSFELVKDLRGGLQAFMWQGLAR
                     LFGSNLKSEYDPNGEDSIIESIVLQIESGTLKIPEAYMTK"
     gene            complement(18129..19607)
                     /gene="accC"
                     /locus_tag="PK736_02335"
     CDS             complement(18129..19607)
                     /gene="accC"
                     /locus_tag="PK736_02335"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRC32263.1"
                     /translation="MEIKKILVANRGEIALRIMRSAKEMGIKTVAVYSEADRAALHVQ
                     YADEAVCIGPAASAQSYLRGDKIIEKALKLGVDAIHPGYGFLSENSDFCRSVTKAGII
                     FIGPPPEAMDGMGLKLSAKATAKKCNVPLVPGTDEAITDIQLAKKIAIEIGFPVLIKA
                     SAGGGGKGMRIVDHESHFEEMLQMAQSEAQSSFGNNAVFIEKYVVGPRHIEIQLLGDK
                     HGNMMYLFERECSIQRRYQKVIEEAPSSCLTPAIRKQMGECAVRVAKEVGYYNAGTVE
                     FLVDINLDFYFLEMNTRLQVEHPVSEIIVGLDLVKEQIKIAQGEKLAYIQEDLKINGH
                     ALEVRVYAEDPANNFLPDIGTLQTYQTPQGNGVRVDDAYIQGMEIPIYYDPMIAKLIT
                     HGVDRNEAISKMIRAIDDYRINGIETTLSFCKFVLKHEAFVSGNFDTGFIKQYFKPEY
                     LKDEKNEADELAAVIANMVFENNTSANEKTVQLQRTKWKANR"
     gene            19874..20695
                     /locus_tag="PK736_02340"
     CDS             19874..20695
                     /locus_tag="PK736_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32264.1"
                     /translation="MKKQSLLVCAFALTLFITSNAFSQAPVTKGGIPVLPAVKSWGVS
                     IDASPFLNYAGNIFNHTDNTAPDFGFTKNYPLSLTALYVKSEKVTYRTNLRLAINTIS
                     VDTFVLLGGSNANQLTTTNTYKQSQSNVTLGLGVQHHKGERRFQGIYGYEGVIKLGTA
                     KQSYKYGEALSDSVNPAFGDRVTLIKQGSTIGVGARGFLGVQYFFAPGISVSAEYGWG
                     FLFQSQGKGEIREERWDTDNGSGEVKKKTTPLPKNSTFKLDNDTGSGAINLNFYF"
     gene            20771..21424
                     /locus_tag="PK736_02345"
     CDS             20771..21424
                     /locus_tag="PK736_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32265.1"
                     /translation="MKHKIFILCLTLITLVATSMSAQSNKKKSKKEEEDATKPRTIIT
                     LGHGVGAFANSIYDSIVEWQFKQSSSTLGPYFLKGERIYKNGFGFGINLAYFQLRQEG
                     LIDRFNEFNDQVQGRYTYNTFSVLMRTNYHFTNIFGKFVPYIGLGLGYRYGAVSYKDN
                     WSETDDYGYPNNPLFPLGFDGTLGFRYYVNDQLGIYSEVGIAKSILQFGMSYKFKPQ"
     gene            complement(21454..21918)
                     /gene="smpB"
                     /locus_tag="PK736_02350"
     CDS             complement(21454..21918)
                     /gene="smpB"
                     /locus_tag="PK736_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRC32266.1"
                     /translation="MKSEKPVIVVSNRKAEFEYFLTERYEAGLVLSGTEIKSIRNHEA
                     GLNEAYCMFMNGELFVRGMHIAHYKEGTYNNHDEKRDRKLLLNKNEIKKLLLKVKDKG
                     TTIIPVKLYFNERSFIKLEIAIARGKKKVDKRNSIKEKDQKREAKRSLKGMD"
     gene            22500..23156
                     /locus_tag="PK736_02355"
     CDS             22500..23156
                     /locus_tag="PK736_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC32267.1"
                     /translation="MYTKIHSGGAPPRPRIGGSSRPSSFSLTQAPRKKKKKSGEVSIK
                     KIAIVGPESTGKTTLSKMIAAHYKTITVPEYAREYIAQLNRPYGPKDLLPIAKGQIQL
                     ENQLLFQASKVLVCDTNMTVMKIWSEQKYGFCAEWILNVINSRYYDHHFVCTPDMPWD
                     KDPLRENPNNRDELFQLYINEMEKQQVRYTIVKGTPEERLQQVIDVLDKFVLKKPAQV
                     "
     gene            complement(23312..25186)
                     /locus_tag="PK736_02360"
     CDS             complement(23312..25186)
                     /locus_tag="PK736_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC32268.1"
                     /translation="MLKNQKPIKPQAKITVANDKNANKPKGKKQVEVKSNKWLHLAIA
                     AALAIITIISFSSCFDNELTNWDDPDYILDNPIIRNLDGATVKEIFTKPYFGNYQPLH
                     ILSYAIEFHYWKLDPKGYHVLSTILFAITVMLVFYFALSLTSGNVIIATIAAALFGLN
                     AMHVESVAWAAERKDTLYSLFFVASLIYYIKYIKSELKWHHLFFAFVLFVLSLLSKVM
                     AVSLVGVLIMLDYWYDRKLSVRLVIEKILFVIPSVIIGIIQVKSVDQAGTIAKGGSFN
                     FVDKISIACHNLLQYFVKLIAPINLSAFYPYPTLETGKLPWDFYASPIVVILLGAAVI
                     YSMRYTKIFFFAAGFFVACIALVLQYLPVGPTMFSERYTLVPAIAFSFLLAYGVNVII
                     QKWPAMKMPALIVSAVYCAVLAVGTYNRCNVWQTSLTLWDNVLEQFPTVATALNNRGK
                     YYGQVLANYQQAETDLTNSIKADPNYELAYSNRGIVYSITKRYDKALADFDMAIKLKP
                     DYAEAMHNRAIAYSQIGKVQESLKDLNYLVEKHPNSAENWHTRGVTFLQLNQAQKGLD
                     DINRALSINPELAQAYASRVQANYMLGRIADAKRDLQMCNQLGVQINPELANKVNAAN
                     "
     gene            25381..26202
                     /locus_tag="PK736_02365"
     CDS             25381..26202
                     /locus_tag="PK736_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRC32269.1"
                     /translation="MNTNNIYSKDWCNQLFEGKTKSYGAYELRINAAKRTTYGMIIAY
                     ALAASFVIGSMVYEKFNVNSQVVQKELFETTLVDLTELFVPPPPTPEPQLNTAPAAPK
                     APSNQMVNPIVVTDKKVIDDTDDGKLPDDLKDDNTGKIGANTGDTKLPVTGGGGNTNI
                     NVDTTSNVPMRVAEEMPEFPGGIEALTKYLSSHVKYPRQYLEMNVNGTVYLSFVINKK
                     GKVTDISVLKGLHESDAFEIEAMKAVSSMPDWKPGKQSNKEVSVMYTLPVAFRTR"
     gene            26484..27098
                     /locus_tag="PK736_02370"
     CDS             26484..27098
                     /locus_tag="PK736_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32270.1"
                     /translation="MIQQKLFLKIAYMTIALGIAIEISQYILLLILNNDSPLKQFIAD
                     FAGKVTWTYIVCTAIALATALTSNQVKTGLAALLSVPVGLYIAQIIHKQALSKFGLDT
                     AIAGNFSFIPVAIKAIEYGALGMLFPYFIKQEKKFPLFILTGFLAGIIFGLCQVLYKI
                     YSTPAQIPFIKLLPICLNELIIPIGCSCIIYSSKVLTMHLKKSD"
     gene            27193..29025
                     /locus_tag="PK736_02375"
     CDS             27193..29025
                     /locus_tag="PK736_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRC32271.1"
                     /translation="MKKIFAFVNLSLLIICTNLVYSQSPTVQDCPCAIPICQNIYTEV
                     NAYSGQGNLFPEINKTISCLGQGEKNDVWYTFTVQSSGSLAFSITPNVLADDYDWAVY
                     NLTNNSCADIFSNPSIEVACNFAGTPGITGPNGLPGPQNELPIPVLAGQTYVINVSQF
                     SVSPNGYTINFTASTASIIDITAPGVQSVDSNFVCGQNFFQITFTENIQCSSVDAADF
                     TLTDPNGTPLTIDSVTSPNCNSVCGYSRVFTFWFNPGANFTGTYNLTLVNTIADICGN
                     AATFPTNFPITVSAYTYNNTITATTCPAGSDGSVAVNVVSPVGNYTYLWSNGSTLSTI
                     NGLSPGTYTVTVSQQGSCSDIQTFIVNGPQPWAITSNTTSTPCNGSLGAASVSVDSGG
                     TAPFTFVWNNGTTGQNINNLTGGVYTVTITDANNCTVTQTITIPQDNNITADFTHKDS
                     LLCAGIEVRFTNISVGGLTYSWNFGDGGTSTDINPIHIYSSGQLYPVTLIAINGACSD
                     TIIDSLLIPELNNILNDVPNIITPNGDGKNDCFTLSSLIDFTKCMNVLIYNRWGEKMF
                     ETSSANNCWNGKNTNGNDVPDGTYFYVLEVNGKKFQGTVAVMRK"
     gene            complement(29302..29775)
                     /locus_tag="PK736_02380"
     CDS             complement(29302..29775)
                     /locus_tag="PK736_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017260153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRC32272.1"
                     /translation="MQTKTQEFYQIDKVDKDILLLLIKDAFIPYTEIAKKLIISSGTV
                     HVRMNKLKEIGIVKGVHIEIDAAKLGYDICAFLGIFLTRGSAYHDVVTRMKTIPEIVE
                     LHYTTGTYSMFAKIICRDTKHLREVLNEKIQEIKAVERTETFISLEESISRQVKL"
BASE COUNT         8950 a         5533 c         5728 g         9679 t
ORIGIN      
        1 attccctttg ctatcactca cttttacaat cacaagttgg ttatgaatgt ttgtgcgaat
       61 attcgctgca ttattattca gcattaaatt ttcattataa agaatcttgc cggtaatatc
      121 tagcacttca attttcaaca attgattatt gtattcatta gtattcgaaa ataaatgcaa
      181 ttgattatcg gtataaaaaa tattgatggc caggttcgaa ttttcgggca acgaatttgc
      241 atttatcact acaaaagaaa tagtatctgt aggtccgggt acaataccgg gagtacaagg
      301 tggcatggct tgagtagcat cgagttgaaa attaaaagtg taagtacccg caggcaaagg
      361 agcaaattta aacacatcgg tttcgtcaca aataacggta agcataccga ggcagtgcat
      421 ggcttgccca tataagttgt tgccattaac aaattgttgt tgatacttga tatcacatcc
      481 accgaacgga aattgacaca tggcattaat agttatgcta tcgttggtgg tagggtaggg
      541 gggtgaaaca tttaacgatt gtatgtactg cgctttgcaa atagtgctgt tcattaccgc
      601 gaatattgcc ataatgaaaa tagaaatttt aaaattgttt ttcatctgta atgtttttag
      661 tgttatacaa agatagacat cctaataaat gtaatggttg ggttgaaaat attttttttc
      721 ctggtcgcta tatttaatat tttttaaata atagttctta cattgcttca ccaatttata
      781 aaatcgtttc tcaaataata ttttaaataa aaagcacaat gaaaaaatca ctattaactt
      841 tattaatttc aattgcattt ttgcaaagca aagcacaggt ttgccagata gattgcttcg
      901 aacaaacaga ttctatcagt caaaatgtaa ccaatctttt gcttaatgga agttttgaaa
      961 gcactacctg tgcaccattc agtgttgggt ggaatgttta ctgcccaaac tccgcagcat
     1021 acaattgcga tgttatgaat tggacctgta ccggtggggg agcttcatct tatccattaa
     1081 tatttagtgc agcctcatcc attattgccg atggtttata tggtgcttat atgggcaatg
     1141 ccttttccga aaattgtggc gctgtggcag gcgatacttc atgccttgta gataataatt
     1201 gcgaggtaac cggattgcca cccggctttc caaacaatgg atcggcttat ggcggcaaca
     1261 ccggggtgag catagagcaa acggtaacag gtcttattcc tggcaattat tatgtgttgg
     1321 aattttggac cgggggcgaa ggttttaata gttatcccaa tcgtggtttg tttggtttag
     1381 atgttggctt tggctataat tatttgcgct gcaaaggaac caccgcactc tcaacagatg
     1441 tgggcatacg ttatgtagtg caatttttgg caaactctac ttcgcatacc attaagttta
     1501 caaactgggg gcatatttgc agttcgtgta cggaggtaat ggtagaccat gtgcgcttat
     1561 atacacaagc acagttgtcg ccattgataa gtccttgtgg cgggtcaggg gcagcgcctg
     1621 ttgtaaatat tgcaagtagc gatacaatat ggtgcgataa acaagccata gattttaccg
     1681 acttatctac aaacatgcct acatcatgga tgtggtattt tactggtgcc acacctagta
     1741 cttcaaccga tcaaaaccct gtgggaattt attacccatc gcaaggcgct ttctcggtta
     1801 cacttgttgc ttgtaatgca ataggttgcg attcggtaac gtttgtaaat tttgtgcagg
     1861 aatttccatc accggtacca cctgtggtaa gtgttataaa cgatagctta tgtgttagtg
     1921 gatatgttac ataccaatgg ttcgaaacag gcaacctcac caatgtgctt tctaccaatt
     1981 catgttttgt gcccacagtt gccggcaatt attttgcgct tgttactgat accaatggtt
     2041 gcgaggtagc ctcgggaacg gtgcctgtaa cggtgtctat atacgagcaa gatgctttgt
     2101 cgaaaataaa aataatacca aatccgttta gcgatattac ctcattggag tttaatattg
     2161 atgatgtgca gactacagca gtagcaattg ttgatgttac aggcagagaa gtaatgactg
     2221 ttgacagcaa aaactttcaa ccaggaaaaa acaaactgca gcttaactta tcgctcgtca
     2281 acgatggaat atatttttgc aaaattggtt cacaaagcaa agtgatgatg gtaaaacttg
     2341 tgaagcgata gtagcgtaaa ggtttgcctt gtgaaaaata tcgctcaata ctattgtttc
     2401 aaatttgtgg ttttggctag tcttctgccc gcattttaat tatttggaga atattatttt
     2461 gtatatcctg cattatccat cgtgcccacc aacttgcata gaaattgaaa tttgtggtga
     2521 gtttaaagtg actgtataat tttaatcggc atgtacttgc attcattcac tataattcat
     2581 aagtgccgtt aagcacatcg aaatacgaac cgccaattac tatttgcttg tccattgttg
     2641 tagaaggaat ttcatgcgaa tcaaaaatta acgtatatag tatacgttgg tgtattgtat
     2701 tttactgcca tttattattt ttataaggat tacgccactc ggggtatttt ttggaagact
     2761 tattgtcgaa attatttgat ggtgatctat tgttctttgt tcggtgaaag gaattgtcct
     2821 gcctgttata tccatcaatt ctattttcac gttctcatcg ttcgatgcat caatattcac
     2881 agtaaggtca tcgccaaaag gattgggaaa tattgtcaaa tcattttctt cttcgttatt
     2941 gaaatatgta gtttgcctcg ccccgttgct tttcacaatt ttaaattgat cgaaaacact
     3001 tcctgtagat gtcaatagtt ttgaagataa agtgtcgcca ttgatttcta aaataagtga
     3061 accataaagg ctttgtgaat aagaatacat ggcagcgtga ggccaagaac cttgtacagt
     3121 ttgccctggc ccgcccttgc ccgatatgcc acaaacggca tacacagtgc ccgtgccacc
     3181 cggcaacgat ttagaataga agggattgtt gccacttgag gtatcaattt tcattgcggg
     3241 agaaattgaa ttttcgaggc caaagtttcc atgcatcagt atactgcgct cgtaagtatg
     3301 gctgtgtcca ctaagtacca ggtcgacatc ataactttct aatagggtgc aaatatttgt
     3361 tcgaatatca atcattgcgg tttccgtatc gctattgtgc gtgcccatag agtagggagg
     3421 gtgatgaaaa taagccactt taaattttga tgtattgttc agcaaatcgt tttgcaacca
     3481 ggtatacatt gcgcttgtgt tattgttaag agcaccataa ctatcgagcg aaataaaatg
     3541 tgtattgcca aaatcataag aataatattt tttggaattg gaagctacac caccggcttg
     3601 cgcctgcgta gggacagtaa agaaattgaa ataagggaaa ttagtgccca atgccgaaac
     3661 actcagataa cctgcattag cataatcgtg atttcccggt gcagggtata caggtgtttt
     3721 tttaaatata tcaggatatt gattgaacac agtcgaattg tattcggccg ttgtgcctga
     3781 ttcgtatgca ttatcgccta accaaatcca aaagttagcg cgcttaccat aatttaaata
     3841 agcacttttc acaccagcct gacctgtggt aattttgcca aaatctccgg ttgcccatac
     3901 cgtaaatgaa gcattggaac cagtagttaa agatgtggta aaataatttt tatcgtcacc
     3961 ctgaatgttt tttagcgaac tgccaacggt gtaataatat tttgtgttag gcaaaagtcc
     4021 ggttaatttt atttcatgtt ctgtggtaat ggttgcacta tctacggcac tgccatacga
     4081 caaatccgtg ccataataca ctttgctatt tgtacttata tcgcttgccc aacgtatatt
     4141 gacactgtta ggcgtgagta cctgcaaata tggcccgcgt acaaggttta tagtgggact
     4201 tccaaccaca ccagctacaa acatattaaa gcttatatcg ctgctagatg ctaactgctg
     4261 atgcacctcc actgcaatac agttgcttcc cgatgtaaaa aatgatttgc taacataaaa
     4321 aatgttgtat gctgattcat cggtgccggc aattgccaca ggcgaaagtg ttaaataatt
     4381 gatagcggta acaggcatat tagtccttat aacttcaaca ccatttacat aaaccaccgc
     4441 accatcatcg cgcactaatc ctatttgtat tgaatcatat tgtgaaggat ttgtaacaga
     4501 aaacgtcttg cggaagtatg ttgtgataaa tttgtttgat gatgatggcc cgtagctaac
     4561 aatggtggct tcgtctccat cgccataacc tagttcggca gagccatttg cccaaccagc
     4621 atccacaaaa gaaggcagtt gccaggctgt attttgatta ctgccattgt caagatattt
     4681 ccatgttgca ttttttgcaa ttagagtatc ggtgctaaaa taaacttgcg atgtagtaaa
     4741 tgaattgtaa tcgctccatg cccctgtatt gccaggtgcg cataaagcat gtacatgcca
     4801 cgagtaagtg gtagcactca caagggagtt caatactaaa aaagtatcgt ttacagtcac
     4861 cgttatccat tgcccgttca taccacgata ttcaacctca tattgacccg tgcctgtggt
     4921 tgttttccat tttaatgttg ctgcgtttga tgtaatgttg ctggcaatat tatttatagt
     4981 tgcagggcaa ggtgtaagtg ttgtaaaatt agccgtgttg gattcattgc tatttccgta
     5041 tgcacaaata gcaatcactt tccagttata agttgatgat ggcaagagtg aattcaacaa
     5101 aaaactcgta tcgtttgatg taattgtatc ccatgcagat gcgcttgaaa ttttataaat
     5161 caacttgtaa tttatggcat cttgcacagt actccacgaa acagaacccg aagcggttaa
     5221 aatgtttgtt gcatttaagt taacaggcga ttggcaaaca ggggttagtg tggtaaaatt
     5281 atttatagtt gtaaaactgc ttgagtatgc agcgcaattg gtttttaccc tgtaattata
     5341 agtggtattg taatctaaat ttgcaatggt gattgatgtg tcgttaatag cattaatgct
     5401 tgtccaagtt gtatcggttt gttttttgta atcgagttga tatgtcactg cataaggtgc
     5461 actgttccag cttatcaatg aactgtaggt agttatattt gtagcattgg tatttagggg
     5521 tgcattgcag agagaataaa agccaagtaa ttgcatatcg aaacttaaat cggaattgcc
     5581 actgctgtat tgatgtactt caactgcaat tatattttca ccggcaatca aattagtatt
     5641 gcttattatg taggggaaat attgcgattc ggtcggagga tttattgatg taagcgccct
     5701 tgttttaatt ccaattgtag tgagtggcaa gtttgttctt tttacttcta taccatttaa
     5761 ataaattaca gcaccatcat cgcacaacat atttatccgc aacgaatcgt aaatagccgg
     5821 attggcgatg tttatttttt tacgaaaata gtatgcaata tattttgttt tggcgctcac
     5881 accataatag attactgtag cctcatcgcc atcgccataa ccaaattgcc ccgcaccact
     5941 tgcccataaa ctgtcgttat aagttagcag cttccagttc ggaatagtag tgtttacatg
     6001 gtcgtaatac ttccaagttg catttttatg gatgagcgta tcggaatttg caaacaatga
     6061 attggaaaat gctataaaca tcaccaaaat gtgaaaagag aaattatttt tcatagcgta
     6121 aatattttat tcagaaggag gtgaggtaaa gaaagaaaat taccttattc aactgtaaat
     6181 ttataagaca aagaaaagaa aaagtgtgaa tttacttaat agaagttgaa tcttattgta
     6241 ctggagtttg ggtcaagtag tcgttaagag tcaaactata taattcacct ttgtgcttat
     6301 caaaatcgaa agtgcctatg taagtcattc ctaatttttg caatacacga atggagcctg
     6361 aatttagttt catagcacga ccaacaattt tttccatttt aaaattttcg aaacccagct
     6421 ttaaacatgc ttgtgctgct tcggttgcgt agcctttgtt ccagtgtcgt ttgaaaaatc
     6481 ggaaaccaat atcatattcg tttaaatcgg cagaaaattt taatccgcac caacctaaat
     6541 attcatcggt cgctttgtcg attacagccc aacggccaaa cccgtatttg gtgtagtgat
     6601 tataattttg caaaaatatt cttgcagcct caatactttc gaagggtggg tcgccagtat
     6661 attttattac atccggatca agatttagta aataagcatt ctctgcatcg tccgcagttt
     6721 tttcgcgtaa atataatctg gtggtggtga ttattttcat ttcttctttt aactataact
     6781 gtatacggtt ccatttcgaa ttgaaaatct ggcataaaaa cagttacaac ttactcatca
     6841 tcacataatt tgtttccata atgtttcgca ctttttcgcg taaggcagga atgtcatttt
     6901 ctgtcatacc tttagtataa atgggttcgg cccatgtgcc tcttattgtg ccgggcaata
     6961 gcgatagtaa ttttttagga tttaaaagtt tatcggtgtt atataaagta aaaacgcata
     7021 taggcgcctg tgctgcaatt gccattcgaa acgcaccgtc cttaaaaggt agcagtatct
     7081 cttttgtttt attccgagtg ccttctggtg ctaaataaat ggaggtgccg gccttcaatg
     7141 cgtttaccat ttttataata ctaagatggc ggtctttgct atcggcacga tttacggtaa
     7201 tgtacattcg ttttattgca tagccaaaca caggcacttt tgtcatttct tctttcgata
     7261 gatattttac tacatgcggt gttgatatgg cccacagcgg tacatcgagc aacgaacgat
     7321 gattgctaat aaaaacaacc ccttcttctt tgggtatgag atgtttgttt ttgattttta
     7381 ctctgataaa taatactgta tatgttacat gagcccaaac ttgcgatatt ctatgtgcaa
     7441 tgcgcgatga ttttggttgt ggaattatgc cgaacactat tacataaagt ataaatacca
     7501 ccaacgaaat aggtataaaa acaattaatg catagatgga ataaatggta gaagctatat
     7561 tttttaacaa tggatgatat ttaatttacg tgcaaatata gaatgttgct gcaaattgaa
     7621 atgtcgaaac aaaatataaa taattcttat tccagatgtg gtttacggca gcactcatgc
     7681 aatttgttgt atgatgcaga atcggcagaa atattatcgg catcgtaacc aagcatcgaa
     7741 attgctttgc gtattttatc tgcatcggtt tttttgctgt tataaattac tgtcacgttt
     7801 tttgatttta catcgagtgt agcaagtttt acaccttttt caaatcgcaa agcattttca
     7861 attttattct tgcacatatc gcattgtgcc gatgtggcaa tcaccgctgt gtcggtttgt
     7921 gcattactca catttgaaaa tgcagatgtg aaaattataa acaggagcaa aaatttcaaa
     7981 tacttatcca ttttattttg attcaatgat gattgcaaaa ttattaaatt attttcattc
     8041 taaagccggc ataaattcta cggcccataa ttggtcccca tatttgtgtg gcgtcaaatt
     8101 ccctactgaa agggttttcg gcatttataa tgggatgatg ctgtgtaaaa tttgtggcat
     8161 tttctacacc ggcatacaat tcaaatttgc ggaaaacttt tgtcacctgt ccgttcatca
     8221 atgtaaatat tggtgatgtt tgaaatgtcg aaccaccatg atgtccttcg gattgcaaag
     8281 caattttttt acttccatct atctgtatag tataatcaac gcgccatttt ttatttatcg
     8341 attcgtaccc caagttgaac aatcctttgt gccttccaat gaggggtttt tcaatttctg
     8401 tttcgaaata agtgatatgt acatcatcta gtttgtatgc caaccgcatg tccagttgtg
     8461 gaattaaatt gtaattaatg gccaccgaaa aactttgtgc atacgactcg cctttcaaat
     8521 tgtagagttg tataaaatcg cgatcagtgt aaggatcaat aattacctga tttacaaatt
     8581 cggtacgata ggcatcaata tttaacgacc cttcgcggtg agcaattaaa aatttatacg
     8641 tggtgttgat accgtaattc catgcccgct caggcaatag atcttcaagg ggcagcagtc
     8701 ttttgccatc ggccattaat ccaatattgt cggcaataat atttgctgta cgaaagctgt
     8761 taccacctgc aacacgtaca ataaagtttt cggtaaaatt gtattttgca ttcatacggc
     8821 ctgtataaaa cattccaaac tcattatgat aatcggcacg gccaccggca atcactgcaa
     8881 attgctcatt cggtttgtag ttgtattcgg caaatatacc cggcactatt tcgtaattgc
     8941 ctatggaaaa attatcgctt atgtgttgat acactccatc aatattcata taacgaaacg
     9001 aggcaccggc ttttatttta ttgttgcaat tccataactc attcatataa attaaattgg
     9061 cgttgtatga gttttgtatg gcatcatatt ttttcaaacc aaaaaacgaa ttcattttgt
     9121 ggtacacata attcatttgc aaacccaaac ttttattagg atgttctgga aataccatac
     9181 cggttttggt atacatttct actctgttgg tttttatttg cgagccaaaa aataatttgt
     9241 ctttattctc atttgtaaag tattcttttt gtccgccttc gcgcagctct gccaaggctt
     9301 ttattccgaa ttgtccttcg agttttttac cgctatgata tcgccaccgg ttataaaaat
     9361 taacctgttg tttcaatggc atatccaaaa agccatcatc gttgtggtcg cgcttttgcg
     9421 aaaaatattc tgtatgcaac atcatcattg tactcagatg ttcgttcagt ttatgtatgg
     9481 tgtaaaaatt ggtttcggtt tccccaaact gcgatccaaa caaattgata tacgcttttt
     9541 cggcagcctc ttcgggcttt atgtattcta tatttatact gccggcaagt gcatcgtatc
     9601 catttactac agagcctgca cccttgttta tttgtatcga ttccatccac gggcccggca
     9661 cataacccaa tccatacgcc tgcgctaagc cattcatcga tgaaatattt tcgttcagca
     9721 attgtgtata tattcccgac aagccaagta attgaatttc ttttgctccg gttattgcat
     9781 cggtataatt tatagaaacc gatgggttgg tttcgaaact ttctgatagg ttgcagcatg
     9841 ccgcacgcaa caattctttt gaagttatca cttcggtatt tattggttgt agtgtagaaa
     9901 tttttaatcc ttcctgcttg ccttgcacaa ctatctcttt gagttgcttg ccgggtttta
     9961 gcaccaccct cagtatcata tctgtattgt tgcctgttac agtatcgttg gtatatccca
    10021 ccatcgatac aacaattctt atgggtaatg ttttgctttc aagttgaaaa aatcccatgc
    10081 tatcggttag cgtagaaagc aacttgtctt ccgaatagac cgatgcaaag ggtagtggct
    10141 tgtatcccat ttcggcatcg ttcaccagca cctgacctcg gattatagat tgtgcattta
    10201 attgcgcaca cacaaatatg aatataatag aaatgtattt cgacataatt ttagaaataa
    10261 tgaaattgga aaaaaattgt ttttgtaaaa aagaaaacaa tcttcctatg tattccacac
    10321 acataaaagt gcacggaagt tttcattcaa tattaaatga ggttgtaaaa ctattgtaac
    10381 aaatgaatta tgttcatgag agaaaaaatc aaaagcataa gatacatttt cgtgcaaata
    10441 aattggcgga ggttgaatgt ggaaattaga tgcaattaca acggcctgat aatgtaattg
    10501 gaaaaaactg aatttattgt cacagcagtt gtctttctga ttgtttgctg gtaaagaatt
    10561 ttcttcgcag cagcaaggtt cgctgtcttt attgtcgcac tggttatcta caatattttg
    10621 gactgaatgg tgctgtgcca ttttgcaaaa atgctgaaca tgcaccaacc caaaggtaga
    10681 agtgcaaatc agcacaagca ttatccagga aaatattttt tgactgtcga ttttcatcct
    10741 ggcgtaaaaa tattccaaaa tattatgtgg agctatgaat tttgtgttaa gcatatttta
    10801 taaaagatta cattcgcagg ctttaaaatt caagaccaaa actatgagca atgttttagc
    10861 aataaatgcc cgcgaaatac ttgattcgcg cggtaatcca acagttgaag tagatgtgac
    10921 cactaccaac ggcattatag gccgggctgc ggtgccatcg ggcgccagta ccggtaagca
    10981 tgaagcagcc gagcttcgcg acaacgataa aagccggtat atgggcaagg gagttacccg
    11041 tgcagtggat aatgtgaatg gtcctatact ggaagagttg cgtggtttgt cggtatttgc
    11101 tcagcatgat atcgaccatc tgatgatgca acttgatggc acgccaaaca agtcgagcct
    11161 tggggcaaat gctatacttg gtgtatcgtt agccatagcc catgccgcat ctcaggaact
    11221 gggcctgccg ctgtttcgtt atgttggcgg tgtatgtgca aacgaattgc ccatcccaat
    11281 gatgaatatt ctgaatggtg gttcgcatgc tgataattca atcgattttc aggaatttat
    11341 gattatgcca accggtgccg attcgtttgc tgaggctttg cgtatgggtg ccgaaatttt
    11401 tcacaacctt aaaaaagtat tactcgataa aaaactatcg acaaacgttg gagacgaagg
    11461 tggttttgct cccaatctga aatcgaacga agaaggaata caaatggtaa tgaaggctat
    11521 tgaagcagca ggttataagc cgggagaaaa tgtttttatt gctatggatg ctgccgttac
    11581 tgagttttac atcgaggaag aaaagatgta tcattttcat aaatctacag gcgataaact
    11641 ttcatcgagc gatatggtga gtttttggaa ggattgggtt tataaatatc ccatcataag
    11701 tattgaagat ggccttgccg aagatgattg ggatggatgg aaagaactta ctgccgccct
    11761 tggaaataaa atacaattgg tgggcgatga tttatttgtt accaatgtta accgcttgca
    11821 acagggcatt gacaaaggtg ttgccaattc gatactagta aaggtaaatc aaataggatc
    11881 gttaaccgaa actataaata cggtgcaatt ggctaagaac aataattaca caactgtaat
    11941 gagtcatcgc tcgggcgaaa cagaagatac cacaattgca gatttagcag tagcattaaa
    12001 ttgtggacaa ataaaaacag gcagtttatc gcgcagcgac cggatttcga aatacaatca
    12061 attgcttcgc atagaagaag tgttgggaaa ttcggccact tataaaggac gtggtttcaa
    12121 atttgctaag agataattat tcgtgtgata aaaaacttcg caattaattt attgttttaa
    12181 aatatggctg acgaaaattc aaaagtcaat ttgcccgata acgaagacga attcgaagag
    12241 caagagattg agcaggaaat agaagttgat ggagaaccca aattggataa tgatggaaat
    12301 aaggaatttc cggattgggt aagcggccaa agaacttctt cttcggggcg caagcgggta
    12361 aagaaaagga ttaagataaa aaagaaaata aaagtaagga agaagagttc tacaagaaaa
    12421 tataagaaat taatagaacg attgatatgg attttggtga ttttgggatt tattgcggct
    12481 ttggtagttt tagtaaatga gcttgatata aatgatgcta agtataaaaa gaaaaaatcc
    12541 ttcaggtcag aatcagtatt tcaaaaagat ataattagaa cataaatata attttcactg
    12601 taaccattta aataaaagat ggatcgtata aaagaaaaat ttgcgcaaaa agccatacca
    12661 atggccgcgg aaattaaaga gttggtaaag caacatggta attttaaact tggcgagtac
    12721 acaatcgagc aagtatatag cggtatgaaa ggcatgatag gtatgattac cgaaacatcg
    12781 aaacttgatc ccgaagaagg aatacgtttt cgtggatatt caatacctga acttcgtgaa
    12841 aaactgcctc atagtgacga aggccctgag ccattgcccg agggtatgtt ttacctttta
    12901 ctcactggcg acttaccaac aaaccaggat gtaaaagaaa taaacaacga atggatgaag
    12961 cgctcaacag tgcccaagca cgttttcgat atgcttgata tgctaccggc aaaaacgcat
    13021 cctatgactc aatttagcat ggcaattatg gcgttgcaaa cagaaagtgt ttttgcacaa
    13081 cgttatcgcg atggcatgaa taaaaaagaa tactgggatg ccatgttcga agatattctt
    13141 acccttattg cacgtttgcc gcgtattgca gcttatatat atcgtaacac ttataagaat
    13201 ggcgttcata tagaaccaaa tccaaaattg gactgggcgg ccaactttgc tcacatgtta
    13261 ggtttcgata ctcccggaat gcgcaaactc atgcgcttat atcttaccat tcatgttgac
    13321 cacgaaggag gcaatgtatc ggcacatgcc actcaccttg taggttcagc gttgagtgat
    13381 gcttacttat cgcttgcagc aggcatgaat gggcttgcag gacctttaca tggacttgca
    13441 gcacaggaag tagcacgttg ggtaattgaa ttgcgcgaaa agtttaatgg cgttccaaca
    13501 aaagaacaaa ttgaagaata cataaaacaa acccttagcg aagggcgtgt ggttcccggt
    13561 tatggtcatg ctgttttaag aaaaaccgac ccacggtata cagcacagca agattttgca
    13621 aaagagaata tggccaacga tgagttgttc aaaatagtgc agatgctata tgaagtgact
    13681 cccgaaatat tgaaagccac aggaaaaata aaaaacccat ggccaaatgt tgatgcgcat
    13741 tcaggcatat tacttatgca ttatggtctc aacgaatatg atttctatac cgttttattt
    13801 ggagtgtcga gagcaatagg tgtactcaca tctttgttgt gggatcgcgc tttgggttcg
    13861 cctatcgagc gccccggttc ggtaaccact gagtgggtaa agaaaaaaat tgctgaacaa
    13921 gcagcagtaa caccatctta ataattagca aaactaaaat attaaagccg gatttattcc
    13981 ggcttttttt tgaaattttt atgctgaatt gtcttgatga agtacgaatc atctgtactt
    14041 cacctgtctt tttcattcgt gttgctaaag tacgaagcat ctctgctgcg taagcctttt
    14101 tgaattgtct tgctgaggta aaaagcatct ctgctgcgta agtcttccgc gaacgactct
    14161 tttggtgcaa cctctattct ggtagggcgc ttcgttcctc agcattgcaa tacgttagct
    14221 cattcttcaa cattgcaata tgtttgttga tgctcctata ccccgtattt caaaaagaag
    14281 gtagttgtaa aaatcatagc attttttttt ctaaagatat ttttcaagaa agtaaatatt
    14341 taaacacttt ttattttcgt atatttgaaa tctaattata aaacacatca tcatgaaaaa
    14401 aaatctactt atcattattc tgcttttttg ttcttttaat gaatgcaaag cacaatggac
    14461 aaaaattaat ttacctaata actggagtat tgcacaaatg caatttgtaa acgactctgt
    14521 gggttatatg attgccgaca gcattgttgg attatcgctt gcaggaaaag tttacaagac
    14581 ctttgaccgg ggtttaaatt ggtacgaaaa aagtttttat taccaatcgt tcgattattt
    14641 gtattttatt aatgaagata caggttggat ttgtatgacc gatggcggaa ccggaaccat
    14701 tcgaagaact gtaaatggtg gcagtacttg gacaaacatt tgttttaatg caccgcatgg
    14761 acctatgttt tttgttacac ctttagtagg ctatattggt tggattgctg ccggtgcaca
    14821 aggtgctgta ttatacaaaa ctattgatgg tgggataaca tggaacagtg tgggtgttaa
    14881 cctgggttta tcgggattgc aggatatggt gttcgctgat tccgttacgg gttacgtagg
    14941 aggcttatat ggacctactc tgggaaggct agacttaaca gttccctctt acaatgtgtt
    15001 tagcaccagc attgcggtgt ctgacatgga ttacgaaaat gcaagtgctc aattattttt
    15061 taccggtgaa gagttcggtg taaattcggg tttgtacaaa acaacttcaa atggttcacc
    15121 tatcaacctg atactagatg ctcaaagtgg attcggggct tatagtgtaa attgtaaaaa
    15181 tgccaacgaa ataatgtgtg gtgggtatgc tggactgatt gcgttcagca atgatggagg
    15241 tattaactgg aacaccgaaa gcactggctc acaaacttcg tttactgatg tacactgggg
    15301 tagtaattat gctatagctg caggaggcga tggtgaagtg tatcgcaaaa gtttgccgct
    15361 ttcatttatt gaaaacagtg ctgtagatga ttttgctatt tatccaaacc cagccgaaaa
    15421 tattataaat ataaaaacaa agattgaagg agaattaact gtaactatat acgatgtatt
    15481 gcagaaaaaa gttttggaac aaaaaataac tccatgcgag ttttgtactg ttgatgtatc
    15541 agcgttgcaa aaagcatttt atacagttgt actctccaat gctaaaaaag aagtaatagg
    15601 taagcaaaag cttttgttga agtagcaaaa atatattgat tattttttgg gacagaataa
    15661 acgaaacaaa aaaaagccgc aagaagttgc ggctttttac atatatgctt ttagcgattt
    15721 attttccaaa catttttgtg atattgccaa tcataccacc gattccacca gcctgattgc
    15781 caccagtaag cgaacttaca atgccaccga tatcgaaact gctatcgttt gcatcattgg
    15841 ttttgtgtac cagtttgctc ataactgttg gtataagcga ttgtacaata ctgcctgctg
    15901 ctgcattgtt tataccaaat ttttccataa ggttaccagc tacctgacca gatatggcat
    15961 taacaattgg attgctgccg gcattggcac ttccaccgct aaacatactc attatgcctt
    16021 ccaggttacc accaccaacg gcacctttta agccactcat aattccgcta gttgctgttt
    16081 cgatagcagc atcattatgc tcatttggaa ttgcaggatt gttgatgata gcatcgcctg
    16141 cgttctcctt cactaaattc aataaacttt caaacattgt ttttattttt aaatgattaa
    16201 acttcttttt ataattcgga tgtcacattt caattatcat accatcatat gccggcacca
    16261 cattttttgg caattctgcc gctacatcat catgcaaacc taattgatgg cttatgtgtg
    16321 tgaggtaagc gtggccagga ttcacctctt ctattaccgc caatgcttcg tccagtgtaa
    16381 agtgtgaaat atgtttttct ctacgtaagg catttattac aaaatggtcc actcctttaa
    16441 tttctctcat cacgtgtgga gcaataaaat ttgcatcggt aatgtagaca aaattattga
    16501 tgcgaaaccc aaacaccggc aactgataat gcattacctc gaggggttgt aaggtgatat
    16561 tgttaatttg aatattcacc cctgcacgaa tatcgtataa atctatttcg ggaatgccgg
    16621 gataattatt gccatcaaag atgtaaggaa actcacgttt tattgctgcc tgcactcttt
    16681 catgtgcata cacatccact cgttttttgt tgaaatagtt aaacgcacgt atatcgtcaa
    16741 gccctgccag gtgatcttta tgttcgtgtg taaatacaat ggcatcgaga tgttttactt
    16801 gtgagcgtaa catttgttgc ctgaaatcgg ggccggtatc tataacaata ttagtgcctt
    16861 catcttctat caatatagca cttcgcaatc ggttatctcg cgggtttgat gaggtgcaaa
    16921 ttttgcacgg gcatgaaata acaggaacac cttgcgaagt accggttcct aaaaaagtta
    16981 ctttcatttt gtgtgaggtt gaagagaatt aattggcaga ttgcatttcg cttttttcga
    17041 gtgaggtgtc ttcggtatta tctgcatttt cagaattgtt gagcattgca ttgttttcca
    17101 tactctgatt gccaagtaaa tttaaaatct ctacaagtgt gtttatttta ttttcgaggg
    17161 tgacaaattt atttactaca actacttttt ttgtttcgag cacgcagtac cccgatttga
    17221 aattaccttt ttcgtagcgc acctgataac cactttcgtt taactggtct tcaacttttt
    17281 taagaaaatt ttgtgtatac ttcacttatg ttcttttgtg caatgatagc tgtttatgtg
    17341 caatgtgctt aacataagtt ttcaactatg tattaaaaaa gtgtttggac cggcacctgt
    17401 tataccatgt gtttgaaatt atagtcaatg atttttttat tttgtcatat aggcttcggg
    17461 aatttttaag gtgcccgatt caatttgcaa cactatggat tctatgatag aatcctctcc
    17521 attagggtcg tactcacttt ttaagttgct gccaaacaaa cgcgccaaac cctgccacat
    17581 aaaggcttgc agcccaccac gcaaatcttt taccaattcg aatgatgtat ttcccgtttg
    17641 tcggtctatc aattttatta aaatgcgtcc ttgcccaata gtgagctttt ttaaatctcc
    17701 ttcaaattgc tctttcatag ctttctcggt cgatttgata tactctttgc ggtctctttc
    17761 acgtgtcaat gttgccgcat gatcatttat ttccttaagc ttggcagcag caatttttgc
    17821 ataaggatat gcccgcaaca catcacgtct gagtttatta tattttgcaa gattggcagc
    17881 agcttcggga tttaaattgc caaccacttc aatagcgcgc atatatacta caggcatggt
    17941 gtcgccatcg ttgataataa ttgccggcaa tttttgaggt tgagcaatgc atgttacttg
    18001 catagtaaac aataaaacta taaaaaatat ttttttcatc tcagcggtca ttctgtttgt
    18061 tgctttattt caaaatgcgt actataattc tcataacgta tttcattcat tactatttca
    18121 ttagtcggtt agcggtttgc tttccatttt gtgcgttgca actgcactgt tttttcattt
    18181 gctgatgtat tattttcgaa aaccatgttt gctataaccg cagctaattc atcggcttcg
    18241 tttttttcat ccttcaaata ttcaggtttg aagtattgct ttataaaccc tgtatcaaag
    18301 tttcccgaaa caaacgcttc gtgtttcaaa acaaatttac aaaaagacaa tgttgtttct
    18361 attccattaa tacgataatc atctatagcc cgaatcattt ttgatatcgc ctcatttctg
    18421 tcaacgccat gtgtaatcaa cttagcaatc atggggtcgt aataaatggg tatttccata
    18481 ccttgtatat atgcatcatc cacacgcaca ccattgcctt gtggcgtttg atatgtttgc
    18541 aaagttccta tatccggcaa aaaattgttt gccggatctt cggcatacac gcgcacttct
    18601 agcgcatgcc catttatttt taaatcttcc tgaatgtaag ccagtttttc gccttgcgca
    18661 attttaattt gttcttttac taaatcaagc ccaacaatta tttccgaaac aggatgttcc
    18721 acctgcaggc gagtgttcat ttcaagaaaa taaaaatcca gattgatatc gactaaaaac
    18781 tctactgtgc cggcattgta atatcctact tcctttgcta cacgcacagc acattcaccc
    18841 atttgcttgc gtatggctgg tgtgaggcaa gaggaaggtg cttcttcaat taccttttga
    18901 taccttcgtt gaatgctgca ttcgcgttca aataagtaca tcatgtttcc atgtttatca
    18961 cccagcaatt gtatttcgat atggcgcggc cctactacat atttttcaat aaaaacggca
    19021 ttgttgccaa aagatgattg tgcctcgctt tgagccatct gcaacatttc ttcaaaatga
    19081 ctttcatgat caactattcg catccctttg ccaccgccac ctgccgatgc ttttatcaaa
    19141 acaggaaagc caatttcgat agcaattttt tttgcgagct gaatatcggt aattgcttca
    19201 tcggtgcccg gaacaagtgg aacgttacat ttttttgccg tagcttttgc agaaagtttc
    19261 aaccccattc cgtccattgc ttcgggagga gggccaataa aaataatacc tgctttggtc
    19321 actgacctgc aaaaatccga gttttccgat aaaaatccgt aaccgggatg tatggcatca
    19381 acgcctagtt taagggcttt ttcaattatt ttatcgccac gtaagtacga ctgcgcggag
    19441 gctgcagggc ctatgcatac agcttcatcg gcatattgta catgaagcgc agccctatcg
    19501 gcttcgctgt atacggctac ggtttttatc cccatctctt ttgccgaacg cattatgcga
    19561 agggctattt caccgcgatt ggcaaccagt attttcttta tctccatatt attcttccta
    19621 ttcgaaggcg aaggtgttat tttttttgct cttttctatc ttttactcct gtctcttttc
    19681 tattaattat taatatcatt gtggaataat tttaaatctg tatttattat taacacaata
    19741 tttctttggc catgttgttt aatagtcagc tatatttctt ctgcattatt gctcaaaata
    19801 aaattacctc ggtagtgtat caaaataaat ttatcatcgt tctcaaaata gtaaacataa
    19861 aatcaaattc tgtatgaaaa aacaatcatt attagtttgt gcttttgcac ttacattatt
    19921 cattacttcc aatgcatttt cgcaggcacc ggttaccaaa ggcggaatac ctgtattacc
    19981 ggctgttaaa agttggggtg ttagtattga tgcgtcacct tttttaaatt atgccggaaa
    20041 catttttaat cacactgata atacagcacc cgattttggt ttcacaaaga actatccgtt
    20101 atcgcttact gctttatatg taaaaagcga aaaggtaacc tatcgtacaa atttgcgatt
    20161 ggcaattaat acaataagtg tggatacttt tgtgttgctg ggcggtagta atgctaacca
    20221 acttactact accaatactt acaaacaaag ccaaagtaat gttacgcttg ggttgggtgt
    20281 gcagcaccac aaaggcgaac gcaggtttca gggaatttat ggttacgaag gtgttataaa
    20341 attgggtacg gcaaagcaat cttataagta tggtgaagca ttgagcgata gcgttaatcc
    20401 cgcctttggc gatcgtgtta cactaataaa gcaaggttca actattggcg ttggcgcaag
    20461 aggttttttg ggtgtacagt acttttttgc cccgggtata agtgtaagtg ctgaatatgg
    20521 ttggggattt ttatttcaat cgcagggcaa aggcgaaatt cgtgaagagc gttgggatac
    20581 agataatgga agtggcgaag tgaaaaagaa aacaacaccc ttgccaaaaa attcaacttt
    20641 caaacttgat aatgataccg gcagtggtgc tataaattta aacttttatt tttaatgcac
    20701 catatctttt gagtattttt aaacttcaat cttattgaat tgttttaatg gttacttatg
    20761 taatgataaa atgaaacaca aaatatttat actttgctta actttgataa cacttgttgc
    20821 tacgtcaatg agcgcgcaaa gcaataaaaa gaagagcaag aaagaagaag aggatgcaac
    20881 aaagccccgc acaataatta ctcttgggca tggtgtagga gccttcgcaa actctatata
    20941 tgatagcatt gtggaatggc aattcaaaca atcatcaagc acgcttggtc catacttttt
    21001 gaaaggagaa agaatatata aaaatggttt tgggtttggt atcaacctgg catattttca
    21061 actacgccaa gaggggctta ttgacagatt taatgaattt aacgatcagg tgcaggggcg
    21121 atatacctac aatactttca gtgttttgat gcgaaccaat tatcacttca ctaatatttt
    21181 tggaaaattt gttccctaca ttggtttagg attaggttac cgttatggtg ctgtttctta
    21241 caaagataat tggtccgaaa cagatgatta cggctaccca aataatccac tttttccttt
    21301 aggcttcgat ggtacattag gttttagata ttatgtaaac gatcaactcg gtatttattc
    21361 cgaagtagga attgccaaat ctattttaca atttggaatg tcttataaat ttaagccgca
    21421 gtaatttctt ttaaaaaatt tattctaaaa aatttaatcc atacctttca aacttcgctt
    21481 cgcttcacgc ttctgatctt tttcttttat actgttgcgt ttatcaactt tctttttacc
    21541 tcgtgcaatg gcaatttcta atttgataaa tgagcgttcg ttaaaatata atttaacagg
    21601 gataatagta gtgcctttgt ccttaacctt taatagaagc ttctttattt cgtttttatt
    21661 gagcagcaat ttacgatcgc gtttttcatc gtgattgttg taggtccctt ctttgtaatg
    21721 tgcaatgtgc attccacgaa caaataattc gccattcata aacatacaat aagcttcgtt
    21781 gaggccggcc tcatggttgc gtatcgattt tatttcggta ccgctaagaa ctaaacctgc
    21841 ttcatatctt tcggtaagaa agtactcgaa ctctgccttg cggttgctta caacaataac
    21901 tggtttttct gatttcattt ttttattgcc ttgcaaaact attcaaatta gttttgaaag
    21961 ttgagaaagt tttgatggat ggggaagttc ctagagttca agagttgaat agttcaagag
    22021 tcccgacact tcggtcggca tcccgaccga agtgtcggca ttcaatggtt tcattgttat
    22081 attgtttaat tgtttcattg tatttgagtt cgcggtctaa gattttattt ttttagacgc
    22141 ttcgagaata ctcaaagtat aattttgact taatctcttt taattttttt tgacttaact
    22201 ccttttgaat tttttttgat ttaaaccttt tgaattatcc gttttacttt ccacaactgc
    22261 ctattgcaca ttgcgcattt atttattgcc tgttgccaat tgtccattgc ctattgaact
    22321 aaaaccttct aacttttgaa ttaaactttt tctacattat tgataaaaat atcttgcatc
    22381 attcaaacag ttactatatt cgccctcgct gaatatcata tttattatta atagaacatt
    22441 ttttgtaaca gaaagattga tgcctaaaaa gccttctttc aactaattct aacaaagtca
    22501 tgtacaccaa aatacatagt ggtggcgcgc cacctcgtcc gcgtatagga ggaagtagtc
    22561 gtccaagttc attctcgcta acacaggcac cgcgcaaaaa gaaaaagaaa agcggagagg
    22621 tttcgattaa gaaaattgca attgtaggtc ccgaaagcac aggcaaaaca accttatcga
    22681 aaatgattgc tgcgcattac aaaaccataa cagtgcctga gtatgcacgt gaatatattg
    22741 cacagcttaa tcgcccttat ggccccaaag atttattgcc aattgctaaa ggccaaatac
    22801 aactcgaaaa tcaattgttg tttcaggcaa gcaaagtact tgtgtgcgat accaatatga
    22861 cagtaatgaa aatatggagt gagcaaaaat atggcttttg tgctgaatgg attttaaatg
    22921 tgataaatag ccgatactac gatcatcatt ttgtgtgtac tcccgatatg ccgtgggaca
    22981 aagatccact tcgcgaaaat cctaataacc gcgatgagct atttcaactt tatataaacg
    23041 aaatggaaaa acagcaagtt agatatacca ttgttaaagg tacgcccgaa gaaagattgc
    23101 aacaagtgat agatgtactt gataaatttg tattgaaaaa acctgcacag gtttagcgtt
    23161 ttcttcttac agctaatttt taatgttaac tttttctttg agccattttt tatactgaac
    23221 ttttttaaaa agtacaattg aaacattcgt ttctattgct aaaataaatt gcatgccaat
    23281 ccggtaaaat actccgtttg tgaattgatt atcaatttgc agcatttact ttattggcca
    23341 gttcaggatt tatttgtacg ccaagttggt tacacatttg caaatctcgt ttggcatcgg
    23401 ctatgcggcc aagcatataa tttgcctgaa cgcggcttgc atatgcttgt gccagctcgg
    23461 gattgataga tagtgcacga ttaatatcat ctaagccttt ttgtgcctgg ttgagttgca
    23521 aaaaagtaac acctcgtgtg tgccagtttt cggcagaatt gggatgtttt tcaaccaaat
    23581 aatttaagtc tttaagagac tcctgcactt tgccaatttg cgaatatgca attgcacggt
    23641 tgtgcatggc ctcggcataa tcgggtttta attttatagc catatcaaaa tcggcaaggg
    23701 ctttatcata tctttttgtt atgctataaa caatacccct gttcgagtat gccagttcat
    23761 aatttggatc ggcctttatg gaatttgtaa gatcggtttc ggcttgctga taatttgcca
    23821 gcacttgtcc ataatatttt ccacgattgt tgagtgcagt tgctacagtt ggaaattgtt
    23881 cgagaacatt gtcccaaagt gttaaacttg tttgccaaac attgcatcgg ttgtaggtgc
    23941 ctactgcaag cactgcacaa taaaccgcag acacaatgag cgcaggcatt ttcattgcag
    24001 gccatttttg aataataaca tttactccat aagccaacaa aaaactaaaa gctattgccg
    24061 gtacaagggt gtaacgctca ctaaacatgg taggaccaac aggaagatat tgcaatacca
    24121 gtgcaataca tgctacaaaa aatccggcag cgaagaaaaa tatttttgtg taacgcatgc
    24181 tatagataac tgcagcacca agaagaataa ctacaatcgg tgaggcataa aaatcccatg
    24241 gtagttttcc ggtttctaaa gtggggtaag ggtagaatgc cgataaattt attggcgcaa
    24301 taagttttac aaaatactgc aacaaattat gacaggcaat gctaatctta tctacaaaat
    24361 taaatgatcc tcctttggca atagtgcctg cctggtcgac cgattttacc tgaataatac
    24421 caatgataac tgacggaata acaaaaagga ttttttcaat tactaatcgt acactcaatt
    24481 ttctatcgta ccaataatct aacattatta atacacctac taatgacacc gccattactt
    24541 ttgataacaa tgacaaaacg aaaagcacaa atgcaaaaaa taaatgatgc cacttcaatt
    24601 cgctttttat atacttgatg taataaatta atgacgccac aaaaaataaa gaatacaaag
    24661 tgtccttgcg ttctgccgcc cacgctacag attcaacatg catggcattg agaccaaaca
    24721 atgccgctgc tattgttgca ataatgacgt tgcccgatgt aagcgaaagc gcaaaataaa
    24781 aaaccaacat tacagtaatt gcaaacagga ttgtactcaa tacatgatag ccttttgggt
    24841 cgagtttcca gtaatgaaat tctatggcgt acgataaaat gtgcaaaggt tgatagttgc
    24901 caaaataggg ttttgtaaaa atttctttta ctgtagcacc atccagattg cgaattatag
    24961 gattgtccaa gatataatcg gggtcgtccc aattggtaag ttcattatcg aaacacgatg
    25021 aaaaactaat gatggtaata atggccaatg cagctgcaat agcaaggtgc aaccatttat
    25081 tgctttttac ttcaacctgt tttttgcctt taggcttgtt ggcattttta tcgtttgcca
    25141 ctgtaatctt agcctgtggt tttatcggtt tctggttttt caacatttta ttcagaggta
    25201 aattttgtca caaaattata ttttaattta taagtagtga tggtttttgc tatgcgcaaa
    25261 atttattatt caacaccagt ttgtcattac attgtaacct taatcaatat caccaaataa
    25321 gattatggaa aaaaagttat taaaacatca aacctacata acaaacaaaa accaaacgtt
    25381 atgaatacga acaacattta ttcgaaagac tggtgtaacc aattattcga aggcaaaacc
    25441 aaaagttatg gcgcttatga attacgcatc aatgctgcca aacgaaccac ttacggaatg
    25501 attatagcat acgcactggc tgcaagtttt gtaataggct ctatggttta cgaaaaattt
    25561 aatgtcaata gtcaggttgt gcagaaagaa ctattcgaaa ccactttagt agatcttacc
    25621 gagttgtttg ttccacctcc accaactcct gagccacaat taaatactgc gcctgctgct
    25681 ccaaaagcac catcaaacca aatggtaaat cccatagttg taactgacaa aaaagtaatt
    25741 gatgataccg atgatggtaa attgcccgat gatttgaaag atgataacac cggaaaaatt
    25801 ggtgcaaata ctggcgacac aaaattaccc gttactggcg gtggtggtaa cactaatatt
    25861 aatgtagata ccacttcaaa tgtacctatg cgtgttgctg aggagatgcc tgaatttccg
    25921 ggtggtatag aggcacttac aaaatattta agctctcatg ttaaatatcc tcgccaatat
    25981 ttagaaatga atgtgaatgg cacggtttac ctttcgtttg taataaataa aaaaggtaag
    26041 gtaacagata ttagcgtact aaaagggttg cacgaaagtg atgcttttga aatagaagca
    26101 atgaaagctg tgagtagtat gcccgattgg aagcccggaa aacaaagcaa taaagaagtg
    26161 agtgtgatgt atactttgcc tgttgctttc cgcacacgat aattaatatt gtgttaattt
    26221 ttgccttatc gcaaacgaaa agtgttttgc aaacgtatat gtaattgtaa tcagcaatga
    26281 ttacacacaa aaacagaatg attctttttc aatcataaaa actaggggta gttttaattc
    26341 aagagcggtg gatgctgaac caaaaggcac caccgcttat ttgttttaaa taatagaaga
    26401 ttccagccca tgtttgattt tttaagtatt tgaaagattg tagttatatt tgaatcttct
    26461 ttacattcta accttgaaag aaaatgattc agcaaaagtt atttttaaaa atagcataca
    26521 tgaccattgc ccttggaatt gctattgaaa tttcacaata tattttattg ttgatactga
    26581 ataatgatag cccattaaaa caatttattg ccgattttgc cggtaaggtt acatggacat
    26641 atattgtgtg tactgcgatt gcgctggcca ctgctcttac aagcaatcag gtaaaaacag
    26701 gattggcagc attgctatcg gtacctgtgg gactatacat tgcacagata attcacaaac
    26761 aagctttgag taaatttggt ttagataccg ctattgcagg aaatttttca tttattcctg
    26821 ttgcaattaa ggctattgaa tatggcgcgc ttggaatgtt atttccgtat tttattaaac
    26881 aagaaaaaaa attccccctt ttcattctta ccggattttt agcaggaatt atttttggtc
    26941 tgtgccaggt tctttataaa atctacagta ctcctgccca aattccattc ataaaattat
    27001 tacccatttg cctcaacgaa ttaattattc ccatcggttg ttcttgcata atatattcat
    27061 cgaaagtgtt gaccatgcat ttgaaaaaaa gtgattaaag gatagaaata ccatagcgtg
    27121 acaaattgtt tttgtacttt gtttaccaaa taaaattttt ccactacctt tagccaaaat
    27181 taatctagct ttatgaaaaa aatattcgct ttcgtcaact tatcactctt aataatttgc
    27241 actaacttgg tttatagtca gtcaccaact gtgcaagatt gcccttgtgc catacctatt
    27301 tgccagaata tttacactga ggtaaatgcc tattccgggc aaggcaattt gttccccgaa
    27361 attaataaaa caatatcatg cttaggccag ggtgaaaaaa acgatgtgtg gtacacgttc
    27421 accgtgcaat cgagcggtag tcttgctttt tctattacac caaatgtttt ggccgatgat
    27481 tacgattggg cagtgtacaa tcttaccaat aatagttgtg ccgatatttt tagtaatccc
    27541 agtattgagg ttgcttgtaa ttttgccggt acaccgggta ttacaggtcc taacggattg
    27601 cccggcccgc aaaacgaatt gcctattccg gttttggctg gtcaaactta tgtcattaac
    27661 gttagtcagt tttcggtttc tcccaacgga tataccatca attttacagc ttccacagca
    27721 agtattatcg acatcactgc gcccggtgtg caaagtgtag attctaattt tgtgtgtggt
    27781 caaaatttct ttcaaatcac ttttaccgaa aacattcaat gttcatcggt tgatgctgcc
    27841 gattttaccc ttaccgatcc caacggcact ccattgacga ttgacagtgt aacatcgccc
    27901 aattgcaact cggtttgtgg ttactcacgt gtatttactt tttggtttaa tccgggtgcg
    27961 aattttaccg gcacttataa tctaacactc gtaaatacta ttgccgatat atgtggcaat
    28021 gctgctacgt ttcctaccaa ctttccgatt acagtttcgg cttataccta caacaatact
    28081 ataaccgcta ctacatgccc tgccggcagc gatggtagtg ttgctgtgaa tgtggtttcg
    28141 cccgttggca attatacata tctttggagc aatggttcta ccctgtccac tataaatgga
    28201 cttagccccg gtacgtatac tgttacggtt agtcagcaag gctcgtgcag cgatattcaa
    28261 acctttattg tgaatggtcc gcaaccttgg gctataacaa gtaacaccac ttcaacgcca
    28321 tgcaatggtt cattgggtgc cgcttcggta tctgtcgatt cgggtggtac tgctccattt
    28381 actttcgtgt ggaataatgg tactaccgga caaaatataa ataaccttac aggcggtgtg
    28441 tacacagtta ccattaccga tgcaaacaat tgtacggtaa cacaaacaat caccattcca
    28501 caggacaata acataactgc cgattttaca cataaagatt cacttctatg tgcaggtata
    28561 gaagtgcgct ttacaaacat atccgttggc ggccttacat attcctggaa ttttggtgac
    28621 ggtggtactt ctactgatat aaatccaatt catatttatt cttcagggca attgtatcct
    28681 gtaacactta tagcaatcaa tggtgcatgc agcgacacta ttatcgattc tttgttgata
    28741 cccgaattga ataatatatt gaatgatgtg cccaatataa ttacacctaa tggcgatggt
    28801 aaaaatgatt gctttacact cagtagcctt atcgatttta caaaatgcat gaacgtactt
    28861 atatacaatc gctggggcga aaaaatgttt gaaacatcat cagccaacaa ttgctggaat
    28921 ggtaagaata caaatggcaa cgatgttccc gatggcactt atttttatgt gctcgaagta
    28981 aacggcaaga agtttcaggg aacagtggct gtgatgagga agtaaagttt tgggttttgg
    29041 tctaaataca tgaggttgga gttttccata tcctacgttt cgagctattt tgaaaagttg
    29101 attttgcaaa caatctaaca gctttttggg atgtctttat ttttgagttc gccaaccaaa
    29161 ccaaaatttt cagcgatcat tggccggcag agattgcatc ttcctatagc aagcggaatc
    29221 taaatgacgc taatgaacaa tgacgattga caagtgtttg agaatgacca gtaactcaat
    29281 aacaaataac caaaaaccaa actacagctt cacttgcctg ctaatacttt cctcaagcga
    29341 tataaatgtt tcggtgcgtt ctacggcttt tatttcctgg attttttcgt tgagtacttc
    29401 acgtaaatgt tttgtgtcgc gacaaattat ttttgcaaac atactgtatg ttccggtggt
    29461 gtaatgcaac tctacaattt cgggaatggt tttcatgcgt gttaccacat cgtgataagc
    29521 agaaccgcgt gtaagaaaaa ttccaaggaa ggcacaaata tcataaccca gttttgctgc
    29581 atcaatctca atgtgcacac cttttactat tccaatttct ttgagcttgt tcatgcgcac
    29641 atgcactgta ccgctcgata tgataagttt ctttgcaatt tcggtataag gtataaaggc
    29701 atcttttatt aatagcagta atatgtcttt atccactttg tcaatttgat aaaattcctg
    29761 tgtttttgtt tgcatttatt tacgatttgt taatgattgt gcaaatatat tatcattatg
    29821 gtaaaaatac aatcaatatg ttattaaaat tgtaatgaac gaatttagta ttgtatgttt
    29881 gccctagaat
//
LOCUS       DAORQA010000014        29318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_372_length_29318_cov_32.043468, whole genome shotgun sequence.
ACCESSION   DAORQA010000014 DAORQA010000000
VERSION     DAORQA010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 29318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29318
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_372_length_29318_cov_32.043468"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..>470
                     /locus_tag="PK736_02385"
     CDS             164..>470
                     /locus_tag="PK736_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRC32273.1"
                     /translation="MKRSELKSLPEYFDRYINKCDDVELLQALQTSIDELNNAPIEKW
                     KALGDTVYATGKWTLKDMLQHLIDMERLFCFRALAFARGETQLLPSCDEDQYAKAAQA
                     "
     assembly_gap    471..542
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            782..1870
                     /locus_tag="PK736_02390"
     CDS             782..1870
                     /locus_tag="PK736_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRC32274.1"
                     /translation="MRAFQLLLLVYVLCIFSSCKNKTETISPETKGITESVYASGTIK
                     SQNQYQVFSKVSGILQTIFVKEGDTIKKGDALFQLNNDNARLSTDNARLAAQTADYIN
                     NKNKLDDLKNIIALARKKLATDSLLFERQKNLWQNKIGSKVDLEQKELNFENAKTNLE
                     SAIYKYDDLQKQLKLASGQSKNNLNISKILQSDLLIKSEVNGRVYSILKEQNELVTSQ
                     TPIAVIGDATSFLVELNIDEHDIVDIKIGQQIIIRMDSYKDKVFEAKLTTLDPIMNER
                     TRTFKAEASFETKPETLYPGLSVEANIVISTKPNALTIPRSYLINDTTVMLENGELQN
                     VKTGLMDYDYVEITSGLSSTSKLILPKK"
     gene            1867..3126
                     /locus_tag="PK736_02395"
     CDS             1867..3126
                     /locus_tag="PK736_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC32275.1"
                     /translation="MKLNLIPDIAKALLLARWKQTLIAAIGVTFSITMFITLLSFMNG
                     LNHLLDGLILNRTAHVRLFNDIKPTEVQPINRWKEYKETHNFVKSIKPANMRPEIYDV
                     EAIMKTLHKDKRVAGVAPKIAAQVFYSLGTIDLNGVINGVDVDEESRLFFFKDYVTTG
                     NYLDLKNVTNSVILGKGAADKMLAKMGDIVTVVTPSGERFPLKVVGYFQSGLADLDKV
                     QSYASMTTTQKLLGKPNNYISDVQVKLYDITLAPALAIEYRKLFGISAEDIQTANAQF
                     ETGSNVRSIISYAVGITLLIVAGFGIYNILNMMIYEKMDTIAILKATGFSGSDVKKIF
                     LTIALSIGIFGAFAGYIFGFLLSNLIDQIPFVTAALPTVKTFPIDYNPKYYLIAGVFA
                     IVTTYFAGWFPARKASAIDPVVIIRGK"
     gene            3128..3826
                     /locus_tag="PK736_02400"
     CDS             3128..3826
                     /locus_tag="PK736_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC32276.1"
                     /translation="MTATKVLEAQSINKSFYDPVKIDILKDINFSINRGEFVSVIGKS
                     GCGKSTLLYILSTMDTDYTGKLFIDGELMEHKTEKQLASIRNEKIGFVFQFHYLLNEF
                     SVLKNVMLPGLKLQKISAQELENKAMELLKILDIEKLALKMANQLSGGEKQRVAIARA
                     MINDPLIIMGDEPTGNLDSRNSAIVFDIFKELTSVYHQTLLIVTHDNSFAQNTQRIIE
                     MDDGRIVTKDALSK"
     gene            complement(3854..4414)
                     /locus_tag="PK736_02405"
     CDS             complement(3854..4414)
                     /locus_tag="PK736_02405"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRC32277.1"
                     /translation="MSLSIFILAAVIQLSAQDEYETCSMEGEAAWEGDTVALQKFVAT
                     CGKIDTLNLDSLHHRAGKNATYQQITMRLNNGKVIRTDSVYFVAETMPQFKSGASDII
                     KYLHDNIRYPAKAMENKISGTVLLSFIVARDGSLTNIRIERGLGSELNVEAIRVIKAM
                     PKWTPGKIKGKPVRVHYTMPIKFKLS"
     gene            5256..5567
                     /locus_tag="PK736_02410"
     CDS             5256..5567
                     /locus_tag="PK736_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32278.1"
                     /translation="MQKNKEEIIDNENNQVVAIQLDSFPLVVGYSWKYYTEMHIADSA
                     GVNYLNNYYDNYWVVLSDTTITGVISTKITQLDSNYNATTHLTFTYYANKPDGLYGMA
                     V"
     gene            5580..6011
                     /locus_tag="PK736_02415"
     CDS             5580..6011
                     /locus_tag="PK736_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32279.1"
                     /translation="MLFLRTKILAQQTQLTLMGSFGNYFAKTDTVFVPDTFLYLLKFP
                     SAINDIWYSHEYNNPAPEIIKRQWLGYKTISTSVGRFNCIKLQMFCDEDHNNQPDFGQ
                     AIIYQYFSNKGLVQEKWIQDINFGNGIAKLTRVTKLVKVNF"
     gene            6344..7297
                     /locus_tag="PK736_02420"
     CDS             6344..7297
                     /locus_tag="PK736_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32280.1"
                     /translation="MKNKLLAFLLSIFVCSANAQLNIGAPGAMRPSSFSKKEMETFKA
                     STSYFVMREKDKAQQTKIEELLKGIWNYNKIEVIDFDAYKKIANNANAIFFGLRSYNS
                     NAEYVSSADLFGKYATYTKVYIQLWQNLEVKKEGHLEETIIARIETTYPWKGADALKE
                     RISIEAFEYLCKNEINIPEWNLGILKNYLQVINQCMNTNTTRWKFEGTVDKEQITKLQ
                     NETLYVNDAVLKTLDPRETESPLKSYTYKYEMINVDDLGAKILSSEKPFYYLMVYKNG
                     SEKITTVVNSKTGEFIYTQYKGMAYYFKEKDILNLLNAISK"
     gene            7725..8195
                     /locus_tag="PK736_02425"
     CDS             7725..8195
                     /locus_tag="PK736_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32281.1"
                     /translation="MVKLIGPPKSLKLLVAHPVSMHDETTQWISELEFYKLEMDFMQN
                     LLNKFFLQFSAKRNLNNFVKVEEKLKAFHKKYYKQMHDDITKHEHNLSELDKDMFAKV
                     RKEIVAEHKKHKTGMKAFMASVKKIKSEIYKLIGDEIKREKGIKKENEDRTLAL"
     gene            8206..9042
                     /locus_tag="PK736_02430"
     CDS             8206..9042
                     /locus_tag="PK736_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC32282.1"
                     /translation="MKNILVPTDFSANAENALTYACELNRKMKTNIKLLHSYSVPVPA
                     GDTLVVPIDDTELKAISLANLTKTRDKIHMQYPDMDLEVQSTMGMAADEIVLIAQETN
                     TELIVMGTHGATGIGAFLLGTNTANVIERTKCAVIAVPENAVFNGLKRIVFAADYGNH
                     NFEQIKHVVEFAKMFGSEIILLHISTGEVEGVFEDMEIARFKEQVISESGYNNVTYRL
                     IEDKDVFHGINQYLEQFKPDMLAISMRSRTLFERMFSRSLTKRLAYQTHTPLLALHIA
                     KK"
     gene            9049..10251
                     /locus_tag="PK736_02435"
     CDS             9049..10251
                     /locus_tag="PK736_02435"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRC32283.1"
                     /translation="MKIKITIIIVALFAFVKIQAQNNVKHKADSIARLYLQNGAGALV
                     IGIYDNGKENIFYYGKISSEKNTAPDGNTVFEIGTLTETFASILYADMLLKNLVNGDD
                     KLKSFLPVNIPSPVYQKIVCKAAIVEDDYPLPNRDAGNIRFTNFMCFPDPSSKPQEIM
                     LCDLATHTTGFPLYPTNFSLKNNKDNPFSNYTKENLYDFLRGYNFDGPFGYDYKHSAT
                     GITLLGHVLSLKADMDFDTLLTTRLFDSLGMHNTRINLTTLQQQNLLPGRNAAGNIVP
                     HFTYDIMAPYGGLHSTANDMMLFLSRNLAKQKDYYVNLLDYTHNGRILLTGKKNVGKE
                     IALGWKINPLDKENKIVWQSNLSGGFATYIGFVETNHTGVFILSSIAKDTQATGESIL
                     KILQTERL"
     gene            10442..11884
                     /locus_tag="PK736_02440"
     CDS             10442..11884
                     /locus_tag="PK736_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRC32284.1"
                     /translation="MVATTEKISLSVSEIIQDQLEQYLIDSLLIIDEDTLQTSQWIYN
                     MFAQNDFLPLWSNEQKLNVDGLQMLNTIDSASFYGLIPDEYRNDILKQKILSVYDSTL
                     QKINATTLYEINILLTDAYFNMAVHIRKGKLDADSLTPVWKGFGSDSLMHYTLNDAME
                     KHNIKASLDSLEPKLYAYKMLKNQLDSLIKLSHPIMPDTLEVIKNDTINIDSINEVRI
                     QQVMLNMERYRWEKPRTEKSHLDINIPSYTLKFYDDDTLVFESSVIIGKYDTQTPWRL
                     NSKITHFYIYPYWNVPYSIATKEILPKLKKDTNYLAKHKMDVLNSKGEILNPSKIKWK
                     KYSEKHFPYKIRQRDGDDNTLGILKFIFSNKYGVYLHDTSTPSLFKKSKRALSHGCIR
                     LEKTKQLAEYLVTNERTVNFNIDSLNENIKNKQRKKVDLVQPLPIHIRYYTCEADSTG
                     IKFYNDIYKLDKKMMDNFFIRKKFEALVQN"
     gene            11862..12488
                     /locus_tag="PK736_02445"
     CDS             11862..12488
                     /locus_tag="PK736_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32285.1"
                     /translation="MKRLFKTKISSIKISALANAKPFENKLQKTISHYNKVVIELILT
                     NKESKAKAKVEAICNLLLENDIGIDDLIKTAVASKEPNKANCIEAIEFATRSNPKIAN
                     LNCLEYVTKTLLEKAPRTKWESAKVIGNIAHLFAAKLDNAINNLLINSEYTGTVVRWS
                     AAFALGEIVKLKTKNNKYLIPAIESIIKREEQSSIRKIYMAALKKVTA"
     gene            12541..13068
                     /locus_tag="PK736_02450"
     CDS             12541..13068
                     /locus_tag="PK736_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC32286.1"
                     /translation="MKKKILIILALAASFCFGFAFKTMIVKQNDTETKPKKVTGIGGI
                     FFKCKDPKKMREWYQKHLGLNTNQYGTVFEWRQAADSSKKGFSQWSPFKETTKYFEPS
                     AKEFMINYRVENLEALVEALKNEGVTVTDTIATYDYGKFVHIMDIENNKIELWEPNDI
                     EYEKLGAQMNLKTTK"
     gene            complement(13304..14023)
                     /locus_tag="PK736_02455"
     CDS             complement(13304..14023)
                     /locus_tag="PK736_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRC32287.1"
                     /translation="MKSEIQFLDGPQSRWDELLFTMKTMLEFIKGFRHLHFVGPCVTV
                     FGSARFVEGDKNYDVARQVGSEIAKLGFTILTGGGPGLMEAANRGAKDVGGKSVGCNI
                     VLSKEQKPNPYLDHWVTIRYFFVRKTLLIKYSYAFIVLPGGFGTMDEYFEAITLIQTH
                     KISAFPVIIIGREFYKDLIAQINTMKQSNTISEDDDKLFQVTDSIEEAINIIKEQSIL
                     KYKLKPHKKIKPWSWLQEGTK"
     gene            complement(14112..14399)
                     /locus_tag="PK736_02460"
     CDS             complement(14112..14399)
                     /locus_tag="PK736_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HRC32288.1"
                     /translation="MQKEEIIIANLKCDGCATTIKNKLSEFEGVTKVDVDNDKDLVTV
                     EHDDKTMREAFTKKLHDLGYPEATEENGLLLQLKSYASCMIGRVSNMTQPN"
     gene            complement(14410..14766)
                     /locus_tag="PK736_02465"
     CDS             complement(14410..14766)
                     /locus_tag="PK736_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32289.1"
                     /translation="MLSHYSYFEEIFDLESILQQGRVKLNIVASELMEELGYASQADF
                     NVALVRSLEVCTALKIPIKNNFRKFYTLRNNELVADWYLSDLAAYLITINGNTCNPNV
                     AKAQLVFMLETKKEKS"
     gene            complement(14750..15694)
                     /locus_tag="PK736_02470"
     CDS             complement(14750..15694)
                     /locus_tag="PK736_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRC32290.1"
                     /translation="MNYKDYYKILGVEKSASQDDIKKAFRKMAIKYHPDKNAGDKKAE
                     EKFKEVNEANEVLGDAEKRKKYDELGENYNQYTQHGGQEGNFDWSKWANRGGGQSTFS
                     GSESFGEGGDFSDFFENIFGQRFNQNTRARQPQGPMRGYDMNAEMEISLEEAYNGGSK
                     QIVLNGQRINLKLAPGTYHGQLLRMKEKGSPGVNGGPPGDLIIDVHIAPHATYKLKGN
                     DLHFDQPLDFKTAVLGGKMNVAIFSKVLSIPIPEGTDSGKIFRLKGLGMPLPNNATEK
                     GDAYVKVMVQVPKNLSPEQKKVFIQFTELNTHSHAKSL"
     gene            complement(15795..16223)
                     /locus_tag="PK736_02475"
     CDS             complement(15795..16223)
                     /locus_tag="PK736_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32291.1"
                     /translation="MQKTKIVKQIGVFMDHAGASLFTPVHDDTVPGEHSRNHHLKHDG
                     ELVDGHQHESPKSTSSESHQHNKEQNELHAFYKNLAQHLKPYDEILVMGPTTAAAEFH
                     NFCTKHGLLNGHDITIEKSNYLTSNQLEEKINHFFKTKLR"
     gene            16439..16870
                     /locus_tag="PK736_02480"
     CDS             16439..16870
                     /locus_tag="PK736_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRC32292.1"
                     /translation="MSLVKRNKLPWGFSPMFSDFFGADDLTFDNAVNRDWVPAVNVAE
                     SDKQFEIEFAVPGMKKEDFKINIENNVLTVSAERKEEKEEKKKNYTRQEYSYNSFTRS
                     FTLPENVKHDGIQARYENGVLTLDIAKEDVVVSKVKEITVG"
     gene            17444..21211
                     /locus_tag="PK736_02485"
     CDS             17444..21211
                     /locus_tag="PK736_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32293.1"
                     /translation="MVWQKTIGGSNSDGATCVKPSSDGGFIIGGYSNSNISGDKTENC
                     NGSWDYWIVKLDAAGNIQWDNTIGGNGDDRVGDIIETYDGGFLVGGQSKSDSSGDKTE
                     DGCNTYINYGDYWVVKLNSSGVIEWQNTIGACAGDFLYSMDHSSDGGYLLGGNSDSNI
                     SCDKTENNNAGSNGGGSGCGGGTGDYWIVKIDSTGNILCQKTLGRGAWASDFLTSVSS
                     TSYGGFICGGGSTAPYGYPVYGGSDNWIIKLNSSCTVQWQKVIGGSGSESFGVIKQIP
                     DGNYIWGANSSSDSSGLKTEPSFGLNDFWILKLDTVGNIIWQKTIGGSGDDNLRYISP
                     TFDNGFICGGYSNSPISGLKTSANFGQNDSWILKLDSVGNIQWQRTIGGSGSDNLSTI
                     SQLAIGNYICGGSSNSNISGNKTENSKGGSDYWVFELDDARNYNLITGKIFFDYNENN
                     VKDSSDIYLPYKIVKDTNTNRFAFSQADGTYRIAVSDTGTYTVAQQNSFYLSSTPPFH
                     NVTFTGMDQIDSVNDFATQQIIFINDLQITITPIGAFRPGFHATYNIHYKNVGTNLAQ
                     GTIVFYPDSSLTYISSSITPVYTSTDSIVWQTPVLYPFYEQNINVTLNVKSTTPLGYW
                     INPLVKIEPMINDQNPTDNYASWSHPVTGSVDPNDILVDKDSITTEELLTSPYLDYII
                     RFQNTGNDTAFVVKILNPIDTFKLDLMSLEIISSSHIFDLNYIPEGNLEFKFENILLP
                     DSNINEPESHGFVRYRIHPKTSLAVHDSVTNYAAIYFDHNLPVITNTALTRIVYPDNY
                     VELFTNVCDSLISPSGNYTWTSTGLYFDTIPGGGTVLDTIYIIHLIVSKSYSYFNAAS
                     CENYTSPSGNYTWTSSGIYSDTLINSAGCDSVLTINLTVNYPSISPLLISECISYQSP
                     SGNYTWTNSGIYYDTIPNEKGCDSILIIDLTIYNPSNSTITISACKNWISPSGNYVWT
                     NSGVYTDTIVNIFGCDSLITINLTVYQSSNSLLSITACESYTSPSNNYVWYNSGIFSD
                     TIPNALGCDSVITIDLTILNNSNAALNITECNNYTSPSGNYSWTTSGIYFDTIPNSSG
                     CDSIVTINLTINSADITVTANPPMLSSNATGANYQWMYCDSIIIQGEVNQIFLASANG
                     SYAVIILQNGCIDTSLCYPVNNVGINEPFYSNHFYIIPNPTSNNLIVSFGSTITKGYI
                     MVSNTLGENVYSQNIFNETKKEINIENISSGIYFVKIFDGEKSYCKKLIVE"
     gene            21872..22399
                     /locus_tag="PK736_02490"
     CDS             21872..22399
                     /locus_tag="PK736_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32294.1"
                     /translation="MAKQTKYHLGIEVDKLTNSIVNTISGDSFPTDVHPATKADLKNT
                     TKKNGWHFNWASEFRLEDREVFKLTIRNNPEIVQGLLNISDLNDHYYLHLIESAPFNF
                     GKNKLYEGVPGNLFAFTCKISWDKGYQGFVSFTSKTKLIDHYERVLGATNVGGHKMVI
                     FPQEALKLIRKYFPS"
     gene            22412..22597
                     /locus_tag="PK736_02495"
     CDS             22412..22597
                     /locus_tag="PK736_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015026133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32295.1"
                     /translation="MGYIKEPPGIDFEVDPTPLTNEDRKSISEIIAYYKKTGRKMHLP
                     KPTKRNSNPTTKKKVIA"
     gene            complement(22778..23491)
                     /locus_tag="PK736_02500"
     CDS             complement(22778..23491)
                     /locus_tag="PK736_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRC32296.1"
                     /translation="MNPALYFSAFVLGLFSSFHCAGMCGPIALMLPQNSGSATKIFLG
                     RFLYNTGRVTTYMVIGIIFGMIGLAASLQGFQKELSVFTGAAIIVSVILTSGSKQRLK
                     AYAVANSYSSPIRKNLKKLFAAKSYVSLFLIGALNGLLPCGFVYLAVAGATAAGSVGG
                     GMLYMALFGLGTFPIMMTISVIANYFGVKFRTIYARISPYMAIALALFLIYRGTDMKT
                     DQCHPQMKAPVANVFCPVH"
     gene            complement(23497..23940)
                     /locus_tag="PK736_02505"
     CDS             complement(23497..23940)
                     /locus_tag="PK736_02505"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRC32297.1"
                     /translation="MKLNWGFGIAMVYIIFAGSMIAFVVVASRQNYDLVSENYYDDAV
                     KYQQTIDAENNGTRKESQLDIVYVQDKNAIQLSGGAQMKNVKGKLSFYKPDKATNDFQ
                     IPFSTDDSSMQTIPLPTVAHGYWSVNANWNVEGKNYSVAKRIFIQ"
     gene            complement(23946..25358)
                     /gene="ccoG"
                     /locus_tag="PK736_02510"
     CDS             complement(23946..25358)
                     /gene="ccoG"
                     /locus_tag="PK736_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRC32298.1"
                     /translation="MSDTKHSDASHDESFRDHISTIDEHGKRAWVYAKKPKGKLYNAR
                     TLVSIGFLIIFFALPWISINGHPFMLLNVIERKFVLLGVAFFPQDFFLFVLAMLTFVV
                     FIALFTALFGRIWCGWACPQTVFMEIVFRKIEYLIEGDMMQQKLLDKREWDNDKILRK
                     VTKHVAFFLLAFIISNTFLQYIIGVDEWKKIVTDGFGSHVGGLIAILVFTGVFYGVYA
                     RFREQVCIVVCPYGRLQGVLLDPNTVVVGYDYNRGEPRGKISKDDEVKHGDCIDCHQC
                     VNVCPTGIDIRNGTQLECVNCTACIDACNDIMVKIKKPEGLIRYTSENAIKKGIKFKV
                     TPRIIGYSAILFVLLTSLITLLAMRSDIETTVLRTPGLMYQKPDATHISNVYNVQLVN
                     KTFKDIPIELKVVEPVGEIKWVGEGITNLEQQGISGGEFFLILPKSAIAKTKTKVKFE
                     VYSNGKKIDDTETSFLGPNN"
     gene            complement(25367..26566)
                     /locus_tag="PK736_02515"
     CDS             complement(25367..26566)
                     /locus_tag="PK736_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HRC32299.1"
                     /translation="MKKKNSIFIKINLLMAAVFFNAMAVSAQATKQAKLELPSMFDNG
                     TLNIAVIFAIVLVLIVLALVKSTVSLSKVLSQNKGKVSAITLMLLSSVSAFSQAVAAA
                     TAKPNPLPDWVFNPAVYLLGFLFTILLVTVYVLYKVNINLLKALNHDTEVVAQEELQT
                     ETEEEKAPGLMRRIYLRMVDSVPVAHEKDILLDHDYDGIKELDNNLPPWWIYGFYVTI
                     IFGVFYLLIYHVSGAGKLQAAEYQQELAVAAQEKEARMRASADNVNEDNVTALADAEL
                     VLKGKETFERLCVTCHLSNGGGTVGPNLTDDYWLHGGGIKNIFKTITHGVPNKGMISW
                     SSQLSPKQIQQVASYVLTFKGKNVAGGKEPQGDKWVEEVVAAKDTTAAKNDTTLSAQV
                     NAKDTIK"
     gene            complement(26576..26749)
                     /locus_tag="PK736_02520"
     CDS             complement(26576..26749)
                     /locus_tag="PK736_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HRC32300.1"
                     /translation="MYKEVLSSINNISIYPVFSFTVFFIFFSMIAVWVIRSKKEDFDA
                     VSNIPLNDNNQTY"
     gene            complement(26761..28887)
                     /gene="ccoN"
                     /locus_tag="PK736_02525"
     CDS             complement(26761..28887)
                     /gene="ccoN"
                     /locus_tag="PK736_02525"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HRC32301.1"
                     /translation="MTLEQFSYDNKIVRNFAVATMVWGIVAMLVGLLIALQLPFPGLN
                     FDAHTTFGRLRPLHTNAVIFAFVGNGMFMGVYYSLQRLMKARMFSDVLSKIHFWGWQL
                     IIVSAALTLPFGITAGKEYAELEWPIDIAIALIWVVFGINMFGTIIKRRERHLYVAVW
                     FYIATVVTVAVLHIVNSIELPISLTKSYSVYAGVQDALVQWWYGHNAVAFFLTTPFLG
                     LMYYFLPKAVNRPVYSYRLSIIHFWALIFLYIWAGPHHLLYTALPDWAQSLGVVFSVM
                     LIAPSWGGMINGLLTLRGAWDKVRDNVELKFFVVAVTAYGMATFEGPLLSLKSVNAVA
                     HFTDWIVGHVHIGGMGWNGFLTFGILYWLIPRIYNTSLFSKKLAGFHFWIGTLGIMFY
                     AIPMYFAGFTQHFMWKEFTEDGVLRYPNFLETVVQIVPMYKLRALGGFLYLTGALVMV
                     YNLVKTAYSGKLIANEAAEAAALEKVYVAHDGSHWHRWIERKPVQLLIASLVVILIGG
                     MVELIPTFMVKSNVPTIASVHPYTPLELQGRDIYVREGCYTCHSQMIRPFRFETERYG
                     EYSKPGEFVYDHPFQWGSKRTGPDLHRQGGRYPNSWHYNHMEDPRTMSPGSIMPPYAF
                     MIEDDLDTTTTAAKIRAMQTLGVPYPDGYDAKANADLMKQADGISANLLKDGIKVMSN
                     KEIVAIIAYLQRMGTDIKGEKNVEQK"
     gene            complement(28921..29091)
                     /gene="ccoS"
                     /locus_tag="PK736_02530"
     CDS             complement(28921..29091)
                     /gene="ccoS"
                     /locus_tag="PK736_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HRC32302.1"
                     /translation="MSVIIVLIAVSLTVAVAFLISFLWAVRSGQYEDDYTPSVRMLFD
                     NEAPEKNTPEEK"
BASE COUNT         9846 a         5478 c         5137 g         8785 t
ORIGIN      
        1 aaataaagag ccactaaatc atcgaaacac caaatcccac aaaacattaa aaatgatttg
       61 gtaacgatta gtgaaatttt gagcctttgt gtttttgtgg caaaaaaata attttcaaag
      121 tgtacttaag aacatgcaat ttaaaattct taaaaataaa aatatgaaac gttcagaatt
      181 aaaatcatta ccagaatatt tcgatcgcta cattaataaa tgtgacgatg ttgaattact
      241 gcaagcattg caaacaagca ttgatgaatt aaacaatgca ccaattgaaa aatggaaagc
      301 actgggcgat actgtttatg caacaggcaa gtggacactt aaagacatgc tgcaacattt
      361 gatagatatg gagcgcttgt tttgttttcg cgccttggca tttgcaagag gcgaaacgca
      421 actgctccca tcgtgcgatg aagatcaata tgcaaaagct gcacaagcca nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nntgcattgt tcgaatcctt tacttctgat atgcttaaca ataccggcaa aggattcaag
      601 ggtgaatatt cagtggcatc cattggtttt attatgccgg gacatcagcg ttggcacctt
      661 ggtgttttgc aagaaaaata tttaccattg ctgggataaa actgatatat atcatataaa
      721 tgtatagttt aatttaattt acttttgccc ggtaaattat ttttaattta attcctactt
      781 tatgagggca tttcaattat tgttattggt ttatgtttta tgtatttttt catcatgtaa
      841 aaataaaacc gaaactattt ctcctgaaac aaaaggtata accgaatcgg tttatgcttc
      901 gggcactatc aaaagtcaaa accaatatca ggtgttttcg aaagtgagtg gcatactgca
      961 aaccattttt gtaaaagaag gcgatacaat aaaaaaaggc gatgcattgt ttcaactaaa
     1021 taacgacaat gcaaggctga gtaccgataa cgcaagactg gcagcccaaa cagcagatta
     1081 cataaacaac aaaaacaaac ttgacgattt aaaaaacatt atagcccttg cgcgaaaaaa
     1141 actcgctacc gattcgcttc tttttgaacg gcaaaaaaat ttatggcaaa ataaaattgg
     1201 ctccaaggta gatttagaac aaaaagaatt gaacttcgaa aacgccaaaa caaatctcga
     1261 aagtgccatt tataaatacg atgatttgca aaagcaattg aaacttgctt cgggccaaag
     1321 caaaaacaat ttgaacatca gcaaaattct tcaaagcgat ttactaataa aaagcgaagt
     1381 gaacggcaga gtgtatagca ttttgaaaga gcaaaacgaa ttggtaacaa gtcaaacacc
     1441 tattgcagta attggtgatg caacatcttt tttagttgag cttaatattg atgaacatga
     1501 catagttgac ataaaaattg gacagcaaat tattataaga atggatagct acaaagacaa
     1561 agtgttcgaa gcaaaattaa caacacttga tcctattatg aacgaacgta cgcgcacatt
     1621 taaggctgaa gcaagtttcg aaacaaagcc cgaaaccctt taccccggcc ttagtgtaga
     1681 agcaaatatt gttatcagta ccaagccaaa tgcattgact attccacgca gttatcttat
     1741 aaatgataca acagtgatgc ttgaaaatgg cgaattgcaa aatgtaaaaa ccggactgat
     1801 ggattacgac tatgtagaaa taacatcagg actaagcagt acatcgaaac taatattacc
     1861 caaaaaatga aacttaacct gatacctgat attgccaaag cccttttatt ggcaaggtgg
     1921 aaacaaacgc tgattgcagc aattggtgta acttttagca ttaccatgtt tattacatta
     1981 ttaagtttta tgaatggatt aaatcattta ctcgatggtt tgattttgaa tcgcactgcg
     2041 catgtaagat tattcaacga tataaaaccc accgaggtgc aacccataaa tagatggaaa
     2101 gaatataaag aaacacacaa ctttgtaaaa tccataaagc ctgcaaacat gcgccccgag
     2161 atttatgacg tagaagcaat aatgaaaacg ttgcacaaag ataagcgtgt ggcaggtgtg
     2221 gcacccaaaa ttgccgcgca ggtattttac agcctcggaa ctatcgattt gaatggtgta
     2281 ataaacggag tagatgtgga cgaggaatcg agactttttt tctttaaaga ttatgttacc
     2341 accggcaatt atctcgattt gaaaaatgtt acaaacagcg tgatacttgg taaaggtgcg
     2401 gccgataaaa tgctggcgaa aatgggcgat attgttaccg ttgttacacc aagtggcgaa
     2461 agatttccac tcaaagttgt aggatatttt caatcgggcc tggccgattt agataaagtg
     2521 caaagctatg caagcatgac caccacacaa aaattgctgg gcaaacccaa taactacata
     2581 agcgatgttc aggtgaaatt gtatgacata acactggcac cagcactggc aatagaatac
     2641 agaaaattat tcggcatatc ggccgaggat attcaaactg cgaatgcaca attcgaaacg
     2701 ggaagcaacg tgcgttcaat aatttcatat gctgtaggca tcacactttt aatagttgca
     2761 ggatttggca tttacaacat tctaaacatg atgatttacg aaaagatgga cactatagca
     2821 atactgaaag ctacaggttt ttcgggaagc gatgtgaaaa agatttttct caccattgcg
     2881 ttaagcattg gaatttttgg cgcctttgcc ggatatattt tcggattttt actcagtaat
     2941 ttaatcgacc aaattccctt tgtaactgca gcactaccaa cagtgaaaac attccccatc
     3001 gattacaatc ctaagtacta tttaatagcc ggtgtgtttg ctattgttac tacctacttt
     3061 gcaggttggt ttccggcacg caaggccagt gctatagatc ctgtagtaat tataagagga
     3121 aaataacatg accgccacca aagtattaga agcacaatcg ataaataaat cgttttacga
     3181 tccggtaaaa atcgatattt taaaggatat aaatttttca attaacagag gcgagtttgt
     3241 ttctgtaatt ggtaaatcgg gttgtggcaa atctacgctg ttatatattt tgtcaactat
     3301 ggataccgat tataccggca aactttttat tgatggcgag ttgatggaac acaaaaccga
     3361 aaaacaactg gcatcaatac gtaacgagaa aataggtttt gtatttcagt ttcattattt
     3421 gcttaatgag ttttcggtat taaaaaatgt tatgttgccg ggattgaaac ttcaaaaaat
     3481 atctgcacag gaattggaaa ataaagccat ggaattattg aaaattcttg acattgaaaa
     3541 gcttgctcta aaaatggcca atcagttatc gggtggtgaa aagcaacgtg tagccattgc
     3601 acgcgctatg atcaatgacc cgctaatcat tatgggcgat gaacctacag gcaacctcga
     3661 tagccgcaac agtgccatcg ttttcgatat ttttaaagaa cttacatccg tttatcatca
     3721 aacgcttttg atagtaactc acgacaatag ctttgcacaa aatacacaac gaataattga
     3781 aatggatgat ggtagaattg taactaaaga tgctttgtca aaataatgtt gacgagaata
     3841 tttcgataca gaattaactc aacttaaatt ttattggcat tgtataatgt acacgcacag
     3901 gtttaccttt tatttttccg ggcgtccatt ttggcatcgc ctttattacc cttattgctt
     3961 ccacattaag ttcactgcca aggccccgtt ctattctaat gtttgtcaat gaaccatcgc
     4021 gggccacaat aaaactaagc aacacggttc ccgaaatttt gttttccatt gcctttgcag
     4081 ggtagcgaat attatcatga agatatttaa taatatcact tgcgccactt ttaaactgcg
     4141 gcattgtttc ggcaacaaaa taaacactat cggtacgtat aaccttaccg ttattgagcc
     4201 gcatggtaat ttgctgatag gtggcatttt taccagcacg atgatggagc gaatctaaat
     4261 ttaacgtatc aatcttacca caagtggcaa caaacttttg caatgcaaca gtatcgccct
     4321 cccatgccgc ctcgccttcc atagagcatg tttcatattc gtcctgtgcc gatagttgta
     4381 taaccgcggc aagaataaaa atagaaagtg acactttttt gagtccgcaa tttttaaata
     4441 tctgtaaatg tcttattacg taagagagct tcaacataga aattggctta ttaaattgta
     4501 aggtgtaaat gtaaacaaat ggcaaaaaca aagaaggaat aatatttgtt tagtgaaagc
     4561 aataaaacca ttttatttac aagtgccaac tattaccgct gttatagaac ttgtaaaatt
     4621 gaaacctcac aaacaataag atagtaaata aaaaacaaga agaaatactg aacccacatc
     4681 aaagctttta tcaataaaat gaaagaaaat aaaagatgta tttctgtgtg gtttaaagga
     4741 aaatcccaat actaaaatat aacatactga agaataaaga acgtttcaac tatccttcag
     4801 gcaaataata acatagcagt ttttatctct caattttcaa cactaaatgc tctgtgccca
     4861 ttataacatc ataagtagtc ctattttgtt gcgcattatt tttgtcgttc tcaataatct
     4921 taattacatt tacgtcacca agtagaacga aacaatgaaa actaaaatga accaaaatat
     4981 gcttccacca tattaataat tattgttttt gcacaacgac tttaaactca aattgaacga
     5041 gaatttgcat ggtgattttt atatttttaa ggatttaata tagcacttca aaattgtcaa
     5101 cttcttaaac acgaaaacaa aaatatgcag ccaaagacta ttcttaaaaa ataaatttgg
     5161 aattcaaatt ggcttcagta cgatattaat agaaataatg aaaatatgat aaaaacaatc
     5221 tgccttttga tgaggtcgtt ttttttaccg agcgcttgca aaaaaacaag gaagaaatta
     5281 ttgacaacga aaacaatcaa gtggtagcaa tccaacttga ttcatttccg cttgttgttg
     5341 gttactcatg gaagtattac accgaaatgc atatagctga ttcggcagga gtaaattatt
     5401 taaacaacta ctatgataac tattgggttg tgctttctga cactacaatt acaggagtta
     5461 tatctacgaa aattactcag ttagacagta actataacgc tacaactcac ttgactttta
     5521 cttattacgc aaacaagccg gacggtttat acggaatggc tgtttaaaat tcgggctctt
     5581 tgctttttct cagaacaaaa atacttgcgc aacaaacaca attaaccctg atgggttctt
     5641 tcgggaatta ttttgcaaaa actgatacag tttttgttcc cgatacgttc ttgtatcttc
     5701 tcaaatttcc atctgccatt aacgatattt ggtattcgca tgagtataat aatcctgctc
     5761 cggaaattat taaaagacag tggcttggtt acaaaactat ttcaactagt gtaggcagat
     5821 tcaattgtat aaagctgcaa atgttttgtg atgaagatca caataatcaa cctgattttg
     5881 gacaagccat aatttatcag tatttctcaa acaaaggatt ggttcaagaa aaatggattc
     5941 aagacataaa ttttgggaat ggaattgcta aactaactcg tgttacaaaa cttgttaagg
     6001 ttaatttcta aatacagcat accaactatt gacaaacatt tttgtatgca ctaaacattc
     6061 gaggtgttaa aaaaacattg tggttggtta tggatgtgag aaaaaaactt ttcaaacaaa
     6121 ctgcaattac aataatggaa caaaacaacg atgattaggt atataaaata gcctcaatag
     6181 cattcgcagt tttcaataat caattgcctc tgtaatacac actgctgaaa aactcttttg
     6241 ccttctgtgt atacagagaa tgaaaaaaac tatttttgct tttgtactac cagtaaaaaa
     6301 gattttttaa aaactaaata attcaatcat aactaatttc tatatgaaaa ataaactatt
     6361 agcatttctg ctcagcatat ttgtttgttc tgcaaacgcg caattaaaca ttggtgctcc
     6421 gggcgctatg aggccgtcta gtttcagcaa gaaagaaatg gaaactttta aagcatcaac
     6481 cagttatttc gtaatgcgcg aaaaggacaa ggcacaacaa actaaaattg aagagttgtt
     6541 aaaaggaata tggaattata acaaaataga agttatcgat tttgatgcct ataaaaaaat
     6601 tgcaaataat gcgaatgcga ttttttttgg tttaagaagt tataattcta atgctgaata
     6661 tgtgagtagt gccgacttat ttggaaagta tgctacgtat acaaaggttt atattcagct
     6721 gtggcaaaat cttgaagtga aaaaagaagg tcaccttgag gaaaccataa tcgcccgaat
     6781 tgaaaccacc tatccatgga aaggtgctga tgcacttaaa gaaagaatta gtattgaggc
     6841 atttgaatat ttatgtaaaa atgaaattaa tattcccgaa tggaatttag gaatactaaa
     6901 aaactatttg caagtgatta accaatgtat gaataccaat acaacccgtt ggaaatttga
     6961 aggtactgta gataaggaac aaattacaaa attacagaat gaaactttat atgtgaatga
     7021 tgctgtatta aagacacttg atccacgaga aacagaatcg cccttaaaaa gttatactta
     7081 caaatatgaa atgataaatg ttgacgactt gggagcaaaa attctttcat cagaaaagcc
     7141 attttattat cttatggttt ataagaacgg atcagaaaaa ataactaccg tggtgaatag
     7201 taaaacaggt gaatttattt atactcaata taaaggtatg gcctattact ttaaagaaaa
     7261 agatatactg aatcttttaa atgctattag caagtaacta atcgcaagtg cttttaagtc
     7321 aaattcatct tcaccatgtt tgaggagttt gaaatcaaaa aaaatccggg caaaaaagat
     7381 tagttttatt ttacagaata aatagttctg ttgtttttcg catatcacat ctatccacct
     7441 tatcatgcat gtacgtaaga aaacgcagag agcattcctg cactattgat aaatcgacaa
     7501 taaaatgctc aatcgattct tgttgctaaa attgaatccg taattcaccc acatacaaaa
     7561 cttatacttt caggtgtgac tttcttttta caaccatctt tttccgagac ttaaattgtg
     7621 cactcctgca tttacataac atgatactta tcatcaataa aactgacaaa agtcatattt
     7681 gccatatgag cagtaaagta catttggaac caaaatatat agcaatggta aagttaattg
     7741 gacctccaaa atcattaaag ctgctagtag ctcatcctgt aagcatgcat gacgaaacca
     7801 cacagtggat ttcggaattg gaattttaca aactggaaat ggattttatg caaaatttac
     7861 tgaacaaatt ttttcttcag tttagtgcaa aaagaaacct caacaacttt gtaaaagtgg
     7921 aagaaaaatt aaaagctttt cacaaaaagt attacaaaca aatgcacgat gacattacaa
     7981 agcatgagca taatttgtcg gaactggata aagacatgtt tgcaaaagtt cgcaaagaaa
     8041 tagttgcaga acacaagaag cacaaaaccg gaatgaaggc ttttatggca agcgtaaaaa
     8101 aaataaaaag cgaaatttac aagcttattg gcgatgaaat aaaacgcgaa aaagggatta
     8161 aaaaagaaaa tgaagaccga acattagcac tataaaaaaa acaatatgaa aaatatatta
     8221 gtaccgacag atttttcggc caatgctgaa aatgcactaa cctatgcctg cgaactaaac
     8281 agaaagatga agaccaacat taaactgctt cactcctact ctgttcctgt gccggctggc
     8341 gatacacttg tagtgcctat agacgataca gaacttaaag caatatcact tgcgaacctg
     8401 acaaaaacgc gcgataagat ccatatgcaa tatcccgata tggatttaga agtgcaatca
     8461 acaatgggta tggccgcaga tgaaattgta ctcattgcac aagaaaccaa tacggaactt
     8521 atagtgatgg gtacacatgg cgcaactggc attggcgcct ttttgctggg aaccaatact
     8581 gccaatgtaa tagaacgtac aaaatgtgct gtaattgcag tgccggaaaa tgctgtgttc
     8641 aacggtctta aaagaatagt gtttgcggcc gactatggca atcacaattt cgaacaaata
     8701 aaacatgtag tagaatttgc aaaaatgttt gggtccgaaa taatactgtt acatatttct
     8761 actggtgagg tggaaggggt ttttgaagat atggagattg cccgttttaa ggagcaggtg
     8821 ataagcgaat cgggatataa caatgtaaca taccgattga tagaagataa ggatgtgttt
     8881 catggcatca atcaatacct cgaacaattt aaacctgata tgcttgcaat aagtatgcgt
     8941 tcgcgcactc ttttcgaaag aatgtttagt cgtagtctta caaaacggtt ggcgtaccaa
     9001 actcatactc cattgcttgc attacacatt gccaaaaaat aatttctcat gaaaataaaa
     9061 atcacaataa ttattgtcgc gctattcgcc tttgtaaaaa tacaggcaca aaataacgtt
     9121 aagcataagg ccgatagcat tgccagacta tatttgcaaa atggcgcagg ggctttggtg
     9181 attggcattt acgacaatgg caaagaaaat attttttatt atggaaaaat ttcatctgaa
     9241 aaaaatactg cacccgatgg caacacagtt tttgaaattg gaacacttac agagactttt
     9301 gcatccattt tatatgccga tatgctattg aaaaatttag tgaatggcga tgataaacta
     9361 aaaagttttt tgcccgtgaa tattccatca cctgtatatc aaaaaattgt ttgcaaggct
     9421 gcaattgttg aagacgacta tccactgcca aaccgcgatg cgggcaatat acggttcaca
     9481 aacttcatgt gttttcccga tccctcatcc aagccacaag aaataatgct ttgcgatttg
     9541 gctacgcata ccacagggtt tccattgtac ccaaccaact tctcgttgaa aaacaataaa
     9601 gacaatccat tttcaaatta tactaaagaa aatttgtacg attttctacg agggtataat
     9661 tttgacgggc cattcggtta cgactataaa cattcggcaa cgggcattac tttgctgggt
     9721 catgtacttt cgttaaaggc ggatatggat ttcgatacac tgcttaccac tcgtttattc
     9781 gattcactcg gcatgcataa tacccgcatt aatcttacaa cattgcaaca gcaaaatttg
     9841 ttgcctggtc gcaatgctgc gggcaatatt gtacctcatt tcacttatga tattatggcg
     9901 ccttacggtg gtttacactc tactgccaat gatatgatgt tgtttctatc gcgtaatctt
     9961 gcaaagcaaa aagactatta tgttaacctg ctcgactata ctcacaatgg aagaatattg
    10021 ctgacgggta aaaaaaatgt tggaaaagaa atcgcccttg gctggaaaat aaatccgctc
    10081 gataaagaaa ataaaattgt atggcaaagc aatctgtctg gtgggtttgc aacatacatc
    10141 ggctttgtag aaaccaatca caccggagtt tttattctat catcaattgc aaaagataca
    10201 caggcaacag gagaatcaat tcttaaaata ctgcaaacag aaaggcttta agtgaggctt
    10261 gccaaaacta tttcctgcaa ccagcattta ttattttgct tcattattgt taataataat
    10321 ttatgttatt attacacaaa tatttttctg cttcagtttt atgcgcaatc aaaattatat
    10381 acttctcgta gtctttcttt ttgtctcgtg tacacacagg catgaaaaac ctccggttgt
    10441 attggtcgcc accaccgaaa aaataagcct atcggtaagc gaaataatac aggaccaact
    10501 agaacaatac ttaatagatt cgttattgat cattgatgaa gatacattgc aaacttcgca
    10561 atggatatac aacatgtttg cgcaaaacga ttttttacct ctgtggagta atgaacaaaa
    10621 actaaatgtt gatggcttac aaatgctaaa cactattgac tcggcaagct tttatgggtt
    10681 gatacctgat gaatatagaa acgatatttt gaaacaaaaa atattatcgg tttacgattc
    10741 caccttacaa aaaataaatg ccaccaccct ttacgaaata aatattttac ttactgatgc
    10801 atactttaat atggctgtgc atatacgcaa aggcaaactc gatgccgatt cgttaacccc
    10861 tgtttggaaa gggtttggca gcgattcgct tatgcattac acattgaatg atgcaatgga
    10921 aaagcacaat ataaaagcat cgctcgattc gttggagccc aaattatatg cttacaaaat
    10981 gcttaaaaat caactcgata gtttgataaa attatcgcac ccaattatgc cggatacgct
    11041 ggaggttatt aaaaacgaca ccattaacat tgattcgata aacgaggtac gtatacagca
    11101 ggtgatgcta aacatggaac gctaccgatg ggaaaaaccg cgtaccgaaa aatcgcatct
    11161 cgatattaat attccatcat acacgctaaa attttatgat gacgatacac ttgttttcga
    11221 gtcaagcgtt attattggaa aatacgatac acaaactcca tggcgcttaa actccaagat
    11281 tacacatttc tatatatatc cttattggaa tgtgccttat agcattgcta caaaagaaat
    11341 attgcccaag ctaaaaaagg atacaaacta tctcgctaaa cataaaatgg acgtgctcaa
    11401 cagcaaaggc gaaatactta atccatccaa aataaaatgg aaaaagtata gcgaaaaaca
    11461 tttcccatat aaaatacggc agcgcgatgg tgatgacaat acactgggaa ttttgaaatt
    11521 tatattcagc aataaatacg gtgtgtactt gcacgacacc agcacaccat cattgtttaa
    11581 aaaatcgaaa cgtgcactaa gccacggttg tatacgtctc gaaaaaacta aacaacttgc
    11641 cgagtacctg gttacaaacg agcgaaccgt aaacttcaac atcgattcgc tcaacgaaaa
    11701 cataaaaaac aagcagcgaa aaaaagtaga cctcgtacaa cctctgccca tacatattcg
    11761 gtattacaca tgcgaagccg attccacagg cataaaattt tataacgaca tttataaact
    11821 tgataaaaaa atgatggata atttttttat aagaaaaaag tttgaagcgc ttgttcaaaa
    11881 ctaaaataag ttcaattaag atttcagcac tagctaatgc aaaacccttc gaaaataaat
    11941 tacaaaaaac aatttcacat tataacaaag tggtcataga attaatttta accaacaagg
    12001 aatcaaaagc caaagccaag gttgaagcta tctgtaatct acttttagaa aatgatattg
    12061 gcatagacga tcttataaaa acagccgtgg caagcaaaga acccaataaa gctaactgta
    12121 ttgaagccat tgagtttgca acaagaagta atcccaaaat agctaatcta aattgccttg
    12181 aatatgtaac aaaaactctt ttagaaaaag caccaagaac taaatgggaa tcggcaaaag
    12241 tgattggcaa cattgctcat ctgtttgcag ccaaattaga caatgccata aacaaccttc
    12301 tgattaacag tgagtataca ggaactgttg tgaggtggag tgcggcattt gctttgggcg
    12361 aaatagttaa actaaaaacc aaaaataata aatatttaat ccctgcaatc gaatccatta
    12421 tcaagcgcga agaacaaagc agcataagaa aaatttatat ggcggcatta aaaaaagtaa
    12481 ctgcatagtt atgtgcagcc atagaaaaaa ctatattaac aaaaaataaa ctcacgtaca
    12541 atgaaaaaaa aaattctaat aattttggca ttggcagcat cattctgttt tggttttgca
    12601 tttaaaacaa tgattgtaaa acaaaacgac acggaaacaa aaccgaaaaa agtaacgggt
    12661 attggcggca tttttttcaa atgcaaagac ccaaaaaaaa tgagggaatg gtaccaaaaa
    12721 catcttgggt taaatacaaa ccaatatggt actgtgtttg aatggcggca agcggcagac
    12781 tccagcaaaa agggattttc gcaatggagt ccatttaaag aaaccacaaa atattttgaa
    12841 ccatcagcaa aggagtttat gataaactac cgggttgaaa atttagaagc attggttgaa
    12901 gcgttgaaaa atgagggagt gaccgtaact gatacaatag caacttatga ttacggaaag
    12961 tttgtgcaca taatggatat agaaaataac aaaatagaat tgtgggaacc aaacgatatt
    13021 gaatatgaaa aactaggtgc acagatgaat ttgaaaacga ccaaataaat tcactaagca
    13081 aaattgtgtt tatacattat cgaatacagg tttattttgc tggcaaatag ttattatcta
    13141 ctcttttaaa taaaaagcaa aaaggtataa atatttttct cgctgcaacc gcacaaagtg
    13201 aattgaacta actcctaaat cgtgtaaatt aattcgtgct tctctcgagt attcgattca
    13261 ccaatgtaag taaaaacgat tgacaactat aaagcggata attttatttc gttccttctt
    13321 gaagccagct ccagggtttt atttttttat gcggcttcaa tttatatttt aaaatacttt
    13381 gctccttaat aatatttatg gcttcttcta tcgaatcggt aacttgaaat aatttatcat
    13441 cgtcctccga aatggtattc gattgtttca tggtatttat ttgtgcaata aggtctttat
    13501 aaaattcgcg accaataata attaccggaa aggcacttat cttatgcgtt tgaataagcg
    13561 ttatggcttc gaaatattcg tccatggtac caaatccacc cggcaatact ataaaggcat
    13621 aggagtattt gatgagcagt gttttgcgta caaaaaaata acggatagta acccaatgat
    13681 cgagatacgg attgggcttt tgttctttcg ataaaacaat attacatcct accgacttgc
    13741 cacctacatc ttttgcaccg cgattagcag cttccattaa gcccgggcca ccacctgtta
    13801 aaattgtaaa acccagcttt gcaatttcac ttccaacttg tcgtgcaaca tcataatttt
    13861 tatctccttc tacaaagcgt gccgaaccaa atactgtaac acaaggcccc acgaaatgca
    13921 aatgcctgaa accttttata aactccagca ttgttttcat ggtgaataat aattcatccc
    13981 atcgcgattg aggaccatca agaaactgaa tttccgattt catctttgtt gctcataatt
    14041 tacaatttaa tagcaaactt aaataataat aaaagctagc cttaagatta taaattaata
    14101 aatgtgattt gttaatttgg ctgtgtcata ttactcactc tgccaatcat acaactggca
    14161 tagcttttca attgtagcag caagccgttt tcttcggtgg cttcgggata acccaggtcg
    14221 tgcaactttt tagtaaatgc ttcgcgcatt gttttatcat cgtgctctac tgttaccaaa
    14281 tctttatcgt tatcgacatc tacttttgtt acaccttcaa actccgatag tttgttttta
    14341 attgttgtag cacatccatc acattttaaa tttgctataa taatttcttc tttttgcatg
    14401 atattttttt taagattttt ctttttttgt ttcgagcata aacaccagtt gtgctttcgc
    14461 tacatttggg ttgcacgtat tcccattaat ggtaataaga tatgctgcaa ggtcgctgag
    14521 gtaccagtct gccaccaact cattattgcg cagcgtataa aatttacgga agttgttttt
    14581 tataggaatt tttagtgctg tacaaacttc gagactacgc acaagagcca cattaaaatc
    14641 ggcctgactg gcataaccca attcttccat cagttcgctg gccacaatgt ttaacttcac
    14701 ccggccttgt tgcaaaatac tttctaaatc gaaaatttct tcgaaataac tataatgact
    14761 tagcatgact gtgtgtatta agttcggtaa attgtatgaa aacttttttc tgttcgggcg
    14821 ataaattttt tggcacctgc accatcactt ttacataagc atcacctttt tcggttgcat
    14881 tatttggcaa tggcataccc aatcctttca gtcgaaaaat ttttccgcta tcggttcctt
    14941 caggaatagg tatagaaagc actttagaaa atatggcgac attcattttt ccgccaagca
    15001 ccgcagtttt aaaatcaagt ggctggtcaa aatgcaaatc gttgcctttg agtttataag
    15061 tggcatgagg ggcaatatgc acatcaatga ttaaatcgcc cggaggccca ccgttcacac
    15121 ctggcgaacc tttttctttc attcgcaaca gttgcccatg ataagtgcct ggtgcaagtt
    15181 tcaaatttat tcgctgcccg ttaagtacta tttgtttgct gccaccattg taagcctctt
    15241 cgagcgatat ttccatttcg gcattcatat cataaccgcg cataggtcct tgaggctggc
    15301 gtgcacgcgt gttttgatta aaacgttgcc cgaaaatatt ttcgaaaaaa tcggaaaaat
    15361 ctccaccctc accaaacgat tcgctaccac taaaagttga ttgccctcct cccctgttgg
    15421 cccatttact ccaatcaaaa ttgccttcct gcccaccgtg ctgtgtgtac tgattatagt
    15481 tttcgcctaa ctcatcatat ttttttctct tctcggcatc gcccaatact tcgttggctt
    15541 cgtttacttc tttaaatttt tcttcggctt ttttatcgcc tgcattttta tccgggtgat
    15601 attttattgc catcttccgg aacgcttttt taatatcgtc ctgcgatgca gatttctcta
    15661 ctcccaatat tttataataa tctttataat tcattgttcc aaactttctt tcttttcaac
    15721 atttaattcc atgaagtaac aacaaatttc acctgtacaa aaaccggaaa agcaactatg
    15781 gtatacaggt accgttatcg caatttagtt ttaaagaaat gatttatttt ttcttcaagc
    15841 tgattggaag taaggtaatt cgatttttcg atagtgatgt cgtgaccatt cagcaaacca
    15901 tgcttggtgc aaaagttatg aaactcggct gcagctgtgg ttggccccat aacaagtatt
    15961 tcgtcatatg gctttaaatg ctgagcaagg tttttataaa acgcatgcaa ctcattttgc
    16021 tctttgttat gctgatgcga ttcgctgctt gtcgatttgg gcgattcatg ttggtgacca
    16081 tctaccaact caccatcatg tttcaggtga tgattgcggc tatgttcgcc cggcactgta
    16141 tcgtcatgta cgggagtgaa taaacttgct ccggcatgat ccataaacac gccaatctgt
    16201 tttacaattt ttgtcttttg cataaatata tatttaaaat taaaatttaa ttattcatat
    16261 ttttccctca ggaaccactc cctgtgattt atgatacaaa aataaattga ggtgatgcga
    16321 taaaacatga caactgtttt taaaaaatat gacataagtc atagtttgca aggaaaaccc
    16381 gaaataaatt tacaccataa ttttgataca aagtctaaca atttaaaaat caattaaaat
    16441 gtctttagta aaaagaaaca agttgccttg gggctttagc ccaatgtttt cggacttctt
    16501 tggtgccgat gacttaactt ttgataatgc cgttaaccgc gactgggtgc cggctgtaaa
    16561 tgttgccgaa agtgacaagc aattcgaaat tgaatttgct gtgcccggta tgaaaaagga
    16621 agattttaaa atcaacatcg aaaacaatgt ccttaccgtt tcggcagaac gaaaggagga
    16681 gaaagaagag aaaaagaaaa attacactcg tcaggagtat tcttataatt cgtttacccg
    16741 ctcgtttact ttacctgaaa atgtaaagca tgatggcata caggcacgct acgaaaacgg
    16801 tgtgttaaca ctcgacatcg caaaggagga tgttgtagta tccaaggtaa aagaaattac
    16861 tgtaggataa ttttgttctg caatttttgg agtgaaggct gattcccaga gggtgtcggc
    16921 cttttttttt gattagctct ttaaccatta aatgcatgaa aaaccgcgaa acgcttttta
    16981 ttatcaatac tttttgtcac cctgttgtca aaccagcttt cgcggctcta atgccaataa
    17041 aaaataaaat aataagattg ccataaagcg actcagatat tcgaacagtt cgagtgtaat
    17101 cactgcaggc tctgcaaaag gtgcatttat cttttacaaa aaataatttt cggatatatt
    17161 tttcgatact ttagctgcgt gataaaaaac aaatacgata ctgtgaagtg tgtatgttta
    17221 gtagttatag gcaacacttg catttcgatt gaacagtagt gcaaccttga actaacattg
    17281 aactctaatc atctgcaatc ctgccaataa aagatacaaa ttgtttaatc gcttatttat
    17341 acttaatttt acaattatga atagaacact accttccgat ttgtttaaaa gagttgcatt
    17401 tctttttttt ctaattatcg cttatgggat aaatgcacag aatatggtct ggcaaaaaac
    17461 aattggcgga agcaattctg atggagctac atgtgttaag ccatcatctg atggtggttt
    17521 tattataggt ggttattcaa attcaaatat ttcaggagac aaaacggaaa actgcaacgg
    17581 cagttgggat tactggattg tgaaattgga tgctgcagga aatattcaat gggataacac
    17641 aattggtgga aacggtgatg atcgggtggg cgatatcatt gaaacctatg atggtggttt
    17701 tcttgtaggt ggacaatcaa aatcagatag ttctggtgat aaaaccgaag atggttgcaa
    17761 tacatatatc aactatggtg attattgggt agtcaaatta aattcatcag gagtaattga
    17821 atggcaaaat acaataggcg cttgtgcagg tgacttctta tattcaatgg atcattcttc
    17881 agacgggggt tatttgctgg gtggtaattc agattcaaat atttcatgcg ataaaactga
    17941 aaataataat gctggtagta atggtggagg ttcggggtgt ggaggaggca caggtgacta
    18001 ctggattgta aagatagatt caacaggaaa tattttgtgt cagaaaactt tgggcagagg
    18061 tgcctgggca agtgattttt taacatctgt ttcttctaca agttatggag gttttatttg
    18121 tggtggtggg tcaactgcac cttatggata cccggtttat ggcggttcgg ataattggat
    18181 tataaaactc aactccagtt gtactgttca atggcaaaaa gttatcggag gcagtggatc
    18241 tgaatcattc ggagtcataa aacagattcc tgatgggaat tatatttggg gggcaaattc
    18301 atcatcagat agctcaggtt taaaaacaga accttcgttt ggtttaaatg atttctggat
    18361 tttaaaattg gatacagttg gaaatattat ctggcaaaaa acaattggag gaagtggtga
    18421 cgataatctg aggtatatat ctcccacttt tgataacgga tttatttgcg gaggttattc
    18481 gaactctccc atatcaggac taaagaccag tgcaaatttt ggtcaaaatg attcttggat
    18541 tttaaaactt gattctgtgg gaaatattca atggcagcga actataggtg ggagtggcag
    18601 tgataatctc agtactattt cacagttggc cattggtaat tacatttgtg gtggctcttc
    18661 aaattctaat atatcaggaa ataaaaccga gaactcaaaa ggtggttctg attactgggt
    18721 atttgagctt gatgatgcaa gaaattataa tttaattacc ggaaaaatat tttttgatta
    18781 taatgaaaat aatgtaaagg actcatccga tatttatttg ccatacaaaa tagtaaagga
    18841 taccaacact aaccgttttg ctttttcaca ggctgatggc acgtatcgta tagctgtttc
    18901 tgataccggt acatatacag ttgcgcaaca gaattcattt tatctgagca gtactcctcc
    18961 ctttcacaat gtaacattca ccggaatgga tcaaattgat tcagtaaatg attttgctac
    19021 ccagcaaata atttttataa acgatttgca aataaccata actcctattg gagctttccg
    19081 accaggattt catgcaactt ataatattca ctataaaaat gtcggaacaa accttgctca
    19141 gggaactatt gttttttatc ctgattcaag tctgacatac atttcttcct ccataacacc
    19201 tgtatatact tcaactgatt caatagtctg gcaaacaccg gttttatatc ctttttacga
    19261 gcaaaatatt aatgtaacat taaatgttaa gtcaacaacg cctttaggat attggataaa
    19321 tccattagtt aaaattgagc ccatgattaa tgatcagaat ccaacagata attatgcttc
    19381 atggagtcat cccgtaacag gttcagttga ccccaatgac attttagtag ataaggacag
    19441 cattaccaca gaagaattgt tgacatctcc ttatcttgat tatatcattc gatttcaaaa
    19501 tacgggaaac gacactgcct tcgttgtaaa aattttaaac cccattgata cattcaaatt
    19561 agatttaatg tcgcttgaaa ttatttcttc atcacacatt tttgatttga attatattcc
    19621 ggaaggaaac ctcgaattta aatttgaaaa tattttactc cctgacagca atataaatga
    19681 accggaaagt catggctttg tcagataccg gatacatcct aaaacatctc ttgcagtgca
    19741 tgacagtgtt accaattacg cagcaattta tttcgatcat aatttgccgg ttataacaaa
    19801 taccgcactt accagaatag tgtatccaga caattacgtt gaattattta caaacgtttg
    19861 tgatagtttg atttctccaa gcggtaatta tacttggact tccacaggac tatattttga
    19921 tactattccc ggtggtggaa ctgtattaga cacaatatat ataatccatc ttattgtaag
    19981 caaatcttat agttatttca atgctgcttc ctgcgaaaat tacacttctc catccggtaa
    20041 ttatacttgg acttcatcag gaatatattc ggatacatta ataaatagtg caggatgtga
    20101 ttccgtgctc actattaatc ttaccgtaaa ctatccatca atttctccac ttttaataag
    20161 tgagtgtatt agttatcaat ctccgagcgg caattatacc tggactaatt ctggaatata
    20221 ttatgacacc attccaaatg aaaagggttg tgactctatt ctaattattg atcttactat
    20281 ttataatcca tcaaattcaa caattactat ttcagcatgt aaaaactgga tatcaccaag
    20341 tggcaattat gtatggacaa attcaggagt ctatacagat actattgtaa atatttttgg
    20401 ttgcgattct ttaataacca tcaatcttac tgtttaccaa agctcaaatt cattattaag
    20461 catcacggca tgcgaaagtt atacttcacc aagcaacaat tatgtttggt ataattcagg
    20521 aattttctct gatacaattc caaatgcttt aggttgcgat tcggtaatta ctattgacct
    20581 aactatttta aataattcga atgccgcact taacataact gaatgcaata actacacttc
    20641 accgagcggc aattattcat ggactacctc aggaatttat tttgatacaa tacccaacag
    20701 ttcaggctgt gattccattg tcactataaa tctcacaata aattctgcgg atattaccgt
    20761 tactgcgaac cctcctatgc ttagttcaaa tgcaacaggt gctaattatc aatggatgta
    20821 ttgtgattca ataataattc agggagaagt aaaccagatt tttcttgcct cggcaaatgg
    20881 ttcttatgcg gtgataatat tacaaaatgg ctgcattgat acttcattat gttatcctgt
    20941 taacaatgta ggtattaatg aaccttttta ttcaaatcat ttttatatta tccctaatcc
    21001 aacgtcaaac aacttaattg tttcttttgg gtcaacaatt acgaaaggct acataatggt
    21061 atcgaataca cttggcgaga atgtttattc acaaaatatt ttcaacgaaa caaaaaaaga
    21121 aatcaatatt gaaaatattt cgtcaggaat ttatttcgta aaaatttttg acggagagaa
    21181 aagttactgc aaaaaattaa ttgttgaata aatttaaacc gtataatttc tgacgggcag
    21241 ttgcatacga gaataacgaa tatgactttc actttgccaa acgtgactta tggtttattt
    21301 cgaagcacaa aaaatgaagt ccgagttgca ctgcctaccc ctaccctttt gtcagaggaa
    21361 tcttcggtag aacatgtagt gaaaattgcc gtgttgttcg caaaggtgtt atcgaaattt
    21421 ttagcaatgg aattttcggg ttgaagatga aaatttagct attactgtat acgtagttat
    21481 gttttttatt tttgtaaaat tgtcgtgttt ctcactctat taaaaacaaa aactgaagaa
    21541 tactttaaaa cggaaacaac tctcaacatc tcacagatga aactctctat tatttttaat
    21601 tgtgctgaat actttgaaat ggtcacgcaa aaaaaataca ataaagtgcg ataattaaat
    21661 gaagggataa attctaactc tcacagagtg aataaatatt ttacagtgaa atgttatgta
    21721 tttatcttcg ataaataagt cacattgaaa aataatatat ggaaatggaa gtcaaaacat
    21781 tttgctatta aaagctaaaa ttgctccaaa attctaatta aaaatcaaac atttggttca
    21841 gaattttgga tattttttgt atctttgaac tgtggcaaaa caaacgaaat atcatttagg
    21901 aattgaagtt gacaagctga caaacagtat tgtaaacact atttctggcg acagttttcc
    21961 gacagatgtt cacccggcaa caaaggccga tttaaaaaac actactaaaa aaaatggttg
    22021 gcattttaat tgggcaagcg agtttagact tgaggatcgg gaagttttca agttgaccat
    22081 tcgtaacaat ccggaaattg ttcaaggact attaaacata agcgatttga acgaccatta
    22141 ctatttgcac cttattgaaa gcgctccttt taattttggc aaaaacaaac tttacgaagg
    22201 agttccaggc aatttgtttg cattcacctg caaaatttct tgggacaaag gttatcaagg
    22261 tttcgtttca ttcacttcca agaccaagtt gatcgaccat tatgaaagag ttttgggtgc
    22321 taccaatgtt ggcggccaca aaatggtaat cttccctcaa gaagcattaa aattaattag
    22381 aaaatatttt ccatcttaaa ataaaaactc catgggatac attaaagaac cgccaggcat
    22441 tgacttcgaa gttgacccaa cacctttgac aaatgaagac agaaaaagca taagtgaaat
    22501 aatcgcttac tacaaaaaaa cggggcgaaa aatgcatttg ccaaaaccaa caaaacgaaa
    22561 tagtaatcct actacaaaaa agaaagtgat tgcttgacca gattagttcg accccaaacg
    22621 aactgcacag cataagataa catatagaac ttgatgcagt gcgaagaagt agaaatcaag
    22681 cttatgtgga accttaatcc attttgccgt tatagacttt tatggaataa gttatgtcat
    22741 ttcaaattta atgaatgatt tctcaaattt caaaaaccta atgcacaggg caaaaaacat
    22801 ttgcaaccgg ggctttcatt tgtggatggc attggtcggt tttcatgtcg gtgccacggt
    22861 atattaaaaa caaggccaat gcaattgcca tgtatggaga aatgcgtgca tagatagttc
    22921 taaactttac accaaagtaa tttgctatta cggatatggt catcataatg gggaaagtgc
    22981 cgaggccaaa caaagccatg taaagcatac caccaccaac tgaacctgca gcggttgcgc
    23041 ctgctactgc aaggtataca aacccgcaag gcagcaaacc attgagtgcg ccaataagaa
    23101 aaagcgaaac atacgatttg gcagcaaaga gttttttaag atttttgcga atggggctgc
    23161 tgtatgaatt ggcaacggca tatgccttta accgctgctt actgcccgat gtaagtataa
    23221 ccgaaacaat aatggctgcc ccggtaaata cacttagttc cttttgaaaa ccttgtagtg
    23281 atgctgctaa accaatcatg ccaaaaataa tacctataac catatacgta gttacacggc
    23341 cggtgttata taaaaagcgg ccaagaaata ttttagttgc cgagcctgaa ttttgcggca
    23401 gcatcaatgc aatgggaccg cacataccag cacaatgaaa gctgctgaat aagcccaaca
    23461 caaaagccga aaaatataat gcagggttca ttaatgttat tgtataaaaa tacgtttggc
    23521 tacggaataa ttttttccct ctacattcca gtttgcattt acgctccagt aaccatgtgc
    23581 cactgttggc aatggaattg tttgcataga tgaatcatcg gttgaaaaag gaatttgaaa
    23641 atcattggta gctttatcgg gtttataaaa acttaactta ccttttacat ttttcatttg
    23701 tgcaccacca cttaattgta tggcattctt atcttgtaca taaacaatat cgagttgcga
    23761 ctccttgcgt gttccattat tttcggcatc aattgtttgc tgatacttta cagcatcgtc
    23821 ataataattt tctgatacca aatcgtaatt ttggcgcgat gctactacta caaatgcaat
    23881 catgctgcct gcaaaaatga tgtacaccat cgctattcca aatccccagt ttaatttcat
    23941 atgttttaat tatttggtcc aagaaaagaa gtttcggtat catcaatttt tttaccgttg
    24001 ctgtacactt caaacttcac cttggttttt gtttttgcaa ttgctgattt tggtagtatg
    24061 agaaaaaatt ccccacccga tataccttgt tgttcgagat tggtgatgcc ctcgccaacc
    24121 cattttattt cgccaacggg ctctactact tttaattcga taggaatatc tttaaaagtt
    24181 ttgttcacca gttgaacatt gtacacattg cttatatgtg tagcatcagg tttctgatac
    24241 atgagtcccg gtgtgcgcaa tactgtagtt tctatatcgc tgcgcatggc caacaatgta
    24301 atgagtgatg taagcaacac aaacaatatt gcagagtaac caattatacg aggcgtaact
    24361 ttaaacttaa ttcctttctt gatagcattc tcactcgtat aacgaatcag cccttcaggc
    24421 ttttttattt ttaccattat atcgttgcat gcatcaatac atgcggtgca gtttacacat
    24481 tcgagttgtg taccattacg tatatcgata ccggtaggac acacattcac acactgatga
    24541 caatcgatac aatcgccatg tttcacttcg tcatctttcg atatttttcc gcgtggttcg
    24601 ccacgattat aatcgtaccc aaccacaaca gtattggggt cgagcaatac accttgcagg
    24661 cgaccgtacg ggcatactac aatacatacc tgctcgcgaa agcgcgcata tacgccataa
    24721 aaaaccccgg taaaaaccaa tatggctatc aatcctccca catgcgaacc aaaaccatct
    24781 gtcactattt ttttccactc atcaacacca attatgtatt gtaaaaatgt attggagata
    24841 ataaacgcaa ggagaaaaaa tgcaacatgt ttggtcactt tgcgtaaaat tttatcgttg
    24901 tcccattcac gtttatccaa caatttttgt tgcatcatat ccccttctat aaggtattcg
    24961 attttacgaa aaacaatttc cataaaaaca gtttgaggac aagcccaccc acaccatatg
    25021 cgaccaaaca atgcggtgaa cagtgcaata aaaactacaa atgtgagcat ggccaaaacg
    25081 aagagaaaaa aatcctgagg aaaaaatgcc acacccaaca aaacgaattt acgttcgata
    25141 acattgagca acataaatgg atgcccgttg atggatatcc acggcaatgc aaaaaatatt
    25201 attaaaaaac ctatgcttac taacgtacgg gcattgtaca atttgccttt aggttttttt
    25261 gcatacaccc acgcgcgctt accatgttca tctatagttg aaatatgatc gcgaaacgat
    25321 tcatcatgcg aagcatcgct atgttttgta tcactcaaag cagaaattat tttatagtat
    25381 cttttgcgtt tacctgtgcc gataatgtag tatcgttttt cgcagccgta gtatcttttg
    25441 ctgctacaac ttcttcaacc catttatcgc cctgaggttc tttgccaccg gccacatttt
    25501 tacctttgaa tgtaagcaca taacttgcta cctgctgtat ttgttttggc gataattgtg
    25561 acgaccagct tatcatacct ttattgggca caccatgcgt tatggtttta aaaatatttt
    25621 ttataccacc accatgcaac cagtaatcat cggtaagatt gggacccact gtgccaccac
    25681 catttgaaag atgacatgta acgcataatc tttcgaaagt ttctttacct ttcaaaacca
    25741 attcggcatc ggcaagtgca gttacgttat cttcgtttac attatcggca ctggcacgca
    25801 tacgggcttc tttttcctgt gctgccactg caagttcctg ttgatactcg gcagcttgca
    25861 atttgcctgc acccgataca tggtatatca ataaataaaa aacaccaaat atgatggtaa
    25921 cataaaaacc ataaatccac catggtggca aattattatc gagctctttt ataccgtcat
    25981 aatcatggtc gagcaagata tctttttcgt gcgctaccgg cacactatct accatacgca
    26041 aataaattct gcgcattaag ccgggcgctt tttcctcctc agtttcggtt tgcaattctt
    26101 cctgtgctac cacttcggta tcgtggttca aggcttttag caaattgata tttactttat
    26161 acaacacata cacagtaacg agcaaaatag taaataaaaa tcccagcaga taaacggcag
    26221 ggttaaaaac ccaatcgggc agcgggtttg gcttggccgt tgctgctgcc accgcttgcg
    26281 agaatgccga cacactgctc agcaacatca atgtaattgc gctaacctta cctttatttt
    26341 gcgataaaac tttacttaaa ctaacagtac ttttaaccaa agccaaaaca atcaaaacca
    26401 aaacgatggc aaatattacc gcaatattta atgttccgtt atcgaacatg gacggcaact
    26461 caagcttggc ctgttttgtt gcctgtgcac ttacagccat ggcattaaaa aacactgcag
    26521 ccatcaataa atttattttt ataaatatcg aattcttctt tttcataatc gtaatttaat
    26581 aagtttgatt attgtcgttt aatggaatat tactcactgc atcgaaatct tcttttttcg
    26641 atcttattac ccacactgct atcattgaaa agaaaataaa aaatactgtg aacgaaaaca
    26701 ccgggtaaat gcttatatta tttatacttg ataaaacttc tttatacatg gttattgatt
    26761 ttatttttgt tcaacatttt tttctccttt aatatcggta cccattcttt gcagatatgc
    26821 tataatggca actatctctt tattgctcat tacttttatt ccatctttca ataagttagc
    26881 acttatacca tcggcttgtt tcattaagtc ggcatttgct tttgcatcat aaccgtcagg
    26941 ataaggaaca cctaaagttt gcatagcacg aatttttgcg gctgtagttg tagtgtccaa
    27001 atcatcttcg atcataaatg catatggagg cattatactt ccgggcgaca tggtgcgtgg
    27061 gtcttccata tgattgtaat gccacgagtt tggatacctg ccaccttggc gatgcaaatc
    27121 gggaccggta cgtttactgc cccattgaaa aggatgatcg tacacaaatt ctccgggctt
    27181 gctatactca ccgtatcgtt cggtttcgaa acggaacgga cgtatcatct gtgagtggca
    27241 tgtgtaacat ccttcgcgca cataaatatc acgaccctgc aactcgagtg gtgtatatgg
    27301 gtgtacactt gcaatagtgg gcacattgga ttttaccata aacgttggta tcaactctac
    27361 cataccacca attaaaatta ccaccaaact tgcaatcaac aattgaacag gcttgcgctc
    27421 aatccaacga tgccaatgac tgccatcgtg ggcaacatat actttttcga gtgcagctgc
    27481 ttcggctgct tcgttggcaa ttaattttcc actgtatgca gtttttacaa gattataaac
    27541 catcaccaat gcaccggtta aataaaggaa acctcccaat gcacgtaact tatacatagg
    27601 aacgatttgc acaacagttt ctaaaaaatt ggggtaacgc aacacaccat cttcggtaaa
    27661 ttctttccac ataaagtgtt gtgtaaatcc tgcaaagtac ataggtatgg cataaaacat
    27721 tattcccaga gtgccaatcc aaaaatggaa gccggccaat tttttcgaaa acaatgatgt
    27781 gttgtaaata cgtggaataa gccaatataa aattccgaac gttagaaaac cattccaccc
    27841 cattccacca atgtgaacgt gcccaactat ccagtcggta aaatgcgcaa ctgcgtttac
    27901 agattttaat gaaagcaatg gcccttcgaa agtggccatt ccataggctg ttacagccac
    27961 tacaaaaaac ttcagttcta cattgtcgcg cactttatcc catgcgccac gcagtgtgag
    28021 caatccgttt atcataccgc cccacgatgg tgcaatcaac attaccgaaa aaacaacacc
    28081 aagcgattgt gcccaatcgg gtaaagcagt gtataataaa tgatgcgggc cggcccatat
    28141 gtataaaaat attaatgccc aaaaatgtat aatcgataac cggtaagaat acaccggacg
    28201 attaacagct ttaggcaaaa agtaatacat caaacccagg aaaggagtag taagaaaaaa
    28261 cgccacagcg ttgtggccat accaccattg caccaatgca tcctgcacac cggcatacac
    28321 cgaatagctt tttgttaaac ttattggcaa ctctattgaa tttacaatat gcaacactgc
    28381 aacagttaca acagtggcaa tataaaacca cactgccaca tataaatgtc gctctctgcg
    28441 ctttataata gtaccaaaca tgttgatgcc aaacactacc catattaatg caatagcaat
    28501 gtcgataggc cactcgagct cggcatactc cttaccggca gtaataccaa atggcaacgt
    28561 gagtgcagca ctcacaataa ttaattgcca tccccaaaaa tggattttac taagcacatc
    28621 gctaaacatg cgcgccttca taagccgctg caacgagtag tacacaccca taaacatacc
    28681 attgcccaca aaggcaaaaa ttactgcatt ggtgtgcaac ggtcgtaagc gaccaaaggt
    28741 agtatgcgca tcgaagttca atcccggaaa cggcaattgt aaggcaatga gcaaacctac
    28801 caacatagca acaatgcccc ataccatagt ggcaacggca aagttgcgga caatcttatt
    28861 gtcataacta aattgttcta atgtcataaa cggtttaaaa tttaatattc aacataaaaa
    28921 tcacttttct tcgggggtat ttttttcagg tgcttcgtta tcaaataaca tacgcaccga
    28981 tggtgtataa tcatcttcgt attggccgct gcgcactgcc cataaaaacg aaatgagaaa
    29041 cgctactgca acggttagac tcacggctat tagaactatt attacactca taatggtggc
    29101 aaaagtatgt ggagatataa tataggaaca tgacctatgt tatacaaaaa catgataaat
    29161 gtcatgaata tgatttggtg gaaaaatgtc ccgctgtaga gtcctcattc acttttgttt
    29221 tttttattgg tatgagtggt tcgaaagtta gactaaatga accagaaatt attcggcatt
    29281 cattcgtgca ttccctgaca ctgcggtcgg ggcaggtg
//
LOCUS       DAORQA010000015        29042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_377_length_29042_cov_30.606548, whole genome shotgun sequence.
ACCESSION   DAORQA010000015 DAORQA010000000
VERSION     DAORQA010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 29042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29042
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_377_length_29042_cov_30.606548"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..645)
                     /locus_tag="PK736_02535"
     CDS             complement(127..645)
                     /locus_tag="PK736_02535"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HRC32303.1"
                     /translation="MLICFLFIVSIKTANTQDNVFYEDVLTTENKSLSQSQVFTKASE
                     WLVYSFYSTKNPVFFSNEKTGRIIGNGSFQVSIPQGVNGNTTPMGHVSFVISIFCKDG
                     KSRLVFSSVTHDAEASGFGSGGNLLSIHPVSDSLGLSDKNWKKIQEETTKSFKGISQA
                     LLAYLNATEKDW"
     gene            complement(895..969)
                     /locus_tag="PK736_02540"
     tRNA            complement(895..969)
                     /locus_tag="PK736_02540"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(932..934),aa:Val,seq:tac)
     gene            1372..4944
                     /locus_tag="PK736_02545"
     CDS             1372..4944
                     /locus_tag="PK736_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32304.1"
                     /translation="MKNFLTILFLFITNSLFSQIPIVNIDNTIGGTSTDGAVAMVNTS
                     DHCMVVLGYSYSNLGLDKSEQCFGSSDYWILKLDSAQNIIWQKTYGGSGYDMPSCIAS
                     TSDGGYIIGGTSLSGIDGNKTEPSKGGYDYWILKLDDLGNIEWQKDIGGNYNDFLSGV
                     VQAQDGGYLVAGYSSSPIGGDKTESNLGQEDYWVLKFDSMGNVLWQNTIGGTDIDKCF
                     SVVVDADGGFVLGGYSKSDMSADKSENYIGGNDFWIVQIDSIGNIVWDNTIGGSQEDI
                     FTSICHTNDNGYLIGGYSNSWPSADKTELPQGHDDYWVLKLDNSGNVLWDKTIGSAGY
                     DNLNCIVTAPNNQYLLAGSSEIGSSGDKTEIGYGSTDGWMVLIDSLGNLTEQIDVGGQ
                     STDNLHSVIYDYQSNLIAGFESFSGISGDKSEDANSSDIWVAKFATKYNTIKGNSYFD
                     TNSNLAKDSNEYYLQNKRITENQSGRFRFTNDKGNYKLHVLDTGNFEVEPPPISYWNS
                     VPAKQNVAFNQFNLTDSMNDFVYQPAAIANDLMITAWPNSAFRPGFQASYSIHYKNIG
                     NTILSPTITFYIDQHLSFSSSTIQPSFVFPDSVVYNLPDIWPFQEQSFFIYVTVHNGT
                     PMNTLILSGVTIYPLINDADIENNSVFLNSTIEVWVTNSYDPNNVLVSPDTLYEYELS
                     HPDFIDYIVNFQNTGNDTAFTVRVENVLSPMLDESTFEIVDASNTVTLDYNPVSRLMQ
                     YHFDNILLPDSNVDEPASHGYIHYKIKPRDTLHIGDVINNGAAIYFDFNTPVITDIAK
                     VHIVSFCDKLISLDVSNFNACYGDIITVQNNSIFPYNNYTWTLDTTVISSNEFVSLSG
                     LSVGTHTIQLHIDTLTCHVDATKNIIINALPVSSIVSNGDTLSSNANFVNYQWLNNGI
                     VLQNSTQYTHIVNQNGWYQLMVSDANGCKAISDSSYMFHCNGLLAMNVPDSIVCPDEA
                     FAAYNTSQTEYILDYEWKIDGNFLSSDTAIVVPAQNIGNHQLSLNIDTLNCTSTIVQN
                     INVLQPVIPTFTFNGIAIVCDSGFNGFQWIFNGTPITSANQITFNLLLSGWYSIAAID
                     SNGCTEVSDSIYIDLSASMAEYNNQEITLFPNPSNDILHVKFDKSFENTFATINDMFG
                     NVLISIQLKGTDTPIDVSLLSNGIYVMVVQRTNSVLHKCFINKK"
     gene            5070..10430
                     /locus_tag="PK736_02550"
     CDS             5070..10430
                     /locus_tag="PK736_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32305.1"
                     /translation="MRSEWQSDVGSWGNEYFADMQATSDGGSIAIGTTCNGASANVSI
                     YYGNNDYWLVKYDSAGQLQWEKTYGGYWDDNATSIITTSDGGYLIGGYSSSPIGGNKT
                     VASHQIFDYWVLKLNANGNIQWQFTCGTTDFDYLSSLLQTNDGGYLLAGTSVTLSSLQ
                     KPFAGYGDMDYWIIKLDSNGVKLWDKCYGGADEDLCTSIVKGKDGNVLLGGISKSYIG
                     GNKTEHAKGLHDYWIVKINSTGDIIWQNTIGASQEDYLYSLYSTEDNGFVLGGNSNSS
                     ITNDKTENKIGLQDFWIIKVDSIGSIQWQNTIGGTQDEFIGNIIQNKLGGYTLVGISA
                     SPKGGDKKTINYLVDNWIVFLDSLGNIIEQKTVSTLGNEGDKINLNYYYKTYFINASC
                     KIINTYDGNYILGTTSNSNISLSKAAKLIDPTGNKNFDFWLQKFSSNYNLATGKCFVD
                     VNKNNVFDINEIPIQSMPVSKGTGTTVVYTDTNGNYELPYFQTGNYTYKAPKTYNSLT
                     PTPTKYTVAFSSASSIDSVNNFAYKSTAPFNDLEVSLEPLGIKGAGDTTYYIISYKNN
                     GTASQQPTLVFYTDYNFVFISSSITPDTITTDSLIWNLSTLPSLSKGHLFLKIYEKPN
                     NNYLPSQTKVAIFPIANDVIDSNNVAIWGPLVNNTVSDFYTTQSKNQHFFVTTNDKFT
                     TDFAYSYCHTKNDTCKQLMFEVIIPLGIDFSTFRVVSSTNPIEISYDKPSRFLKIIAN
                     NLLLYKSNTISDTNVFCLHFQLTSDSIIDTIVVSQNIFFVDSLFKFENKTKIIFSQYC
                     DNLAKIIFDDSNVCETDTVKVNNIASIPFSNNFVWVLDSDTVSTNDFFIFYNLVPGNH
                     NVTLIVNSPYCQKIVSHAFSVNSFSSSFKPPIFADLGQVRSWGNPDPRDPFQWYFNGT
                     PVGTNYQSCQMAQAGWYQVFANNIYGCQSFSDSLFAINCFDAMDIQLSDLTPCPHTPL
                     FVSNNGNFKVSAKWWLDLKPIPGDSGLNITSLEPGIHKILLKIDSANCKLYDENKSFW
                     VNEFIIPYLVFVNDTILCDKTFSYYEWFFNGVSIPNNNVQNLHPDSSGYYQLFVIDSL
                     GCSHFTDSIYYHNALGVIHLQLDKTICEGDAFFATNQGNYPYNNNFNWFVDSIFQVTD
                     SALFLGNLPAGNHKITLMVIDYVDTVLLSKFINVNALPVKPVIAFANNVVSCNGSYNV
                     YKWYRNDTLVTYNSSMTIFQAGWYKLRVENTSNCFIYSDSVFLLPCNLYLSLEVVNSG
                     FCIGDTLKAYNKSIFPSTNYWTLDGNFISSDTSISISGISVGWHTLVLEVTANGCTTT
                     TSASIIVSQPVIPLINKFYNDLHCNLSSFSYLWYLNGNPISNSNVQHWIIAASGWYKV
                     SCTNLYSCTSFSDSIFCNLCPPMVNFNVYNDNVCFTDTIIAYSITPMYPSTYWSIDGV
                     FYSSDSLIKIHNVGAGIHNLRLNSDSANCVTSHYRNITFAVDSARFVAQNNLLTSTYN
                     LLSYQWYLNGLPIANATNKTLIIYQSGYYSLSGMANLNCFVFSDTLYIPLCNYTPTFT
                     MSSTSVCMYDTVVCYMNQSPSTYKSWMLNGTFISQDSAINIFNFPPNTNFISLVYSTN
                     FCYRLTTQNFQGVTAIKPTITVFTNQLQTNNVAFANFQWLLNGALIPLATTYIYTPNQ
                     SGYYQVDVTYPNGCSNISDSVYFGLNGFENYHTNHFSIYPSPADENIQIHLFTNAENT
                     NAILLDKHGRIVLSKKLENKISTFETNEISQGVYTLIIEQNNAREFQRIVIVHNADNE
                     SK"
     gene            10433..15844
                     /locus_tag="PK736_02555"
     CDS             10433..15844
                     /locus_tag="PK736_02555"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32306.1"
                     /translation="MSIKSKYLTIKKIALIALCLFAIDCSISFGQSLRYEWQVDLGCN
                     NDDYFSDLEKTPDGGSIVLGTSFGGNTGNKTVYYGDGDYWLAKLDSTGQLQWQKTYGS
                     DSYEVAASVTTTTDGGYIICGFSASDWGGNKTATLYGYYDYWVLKLNAAGYIEWQKSF
                     GTTDGDFAIDLVQTNDGGFIIGGTSDTQTSSNKLFAGYGLKDYWIVKLDSNGTKLWDK
                     CYGGADYDVLTCLIKSDNGNLLLGGYSKSYIGGNKTEHAKGLNDYWIIKIDSIGNIIW
                     QNTIGGSQEDFLFSAFRTSDNGFVLGGNSNSSITTDKTENKIGLQDFWIVKVDSMGNV
                     QWQNTIGGTKDEKIGDVIQTSNGEYVVAGKTVSETGGDRQSINYGSDIWLLFLDTVGI
                     IVDQKIICANKDDGESYICCYQELPNCKLVESNDGGLFLGTSTRSGISLEKNAALTVY
                     GNYYIDFSDYWIIKLNKNYNLANGKCYLDVNKNNVFDGNDKSINGFAVNNNGSQVVFT
                     DNDGNYELTYFDPGTYTYKPPLKYNGISPTPAKLIVSFTNTSTIDSLNNFSYKPPATL
                     QDLEISLTPALLSYYPDTEYFRIEYQNNGTSGQLPIIVFYTDTNFVFISASIASDSIT
                     PDSVIWNLPLLPPNSSGHFYIKVYKKTKSYDNPFITKVVILPIIGDDIDTNNIDIWNA
                     MNSNYDQGFRCTQSATKYYLRYLGDKFTTDFTYSYFKQKIDTCNQIMFEVIIPTYLSF
                     STFKLVSSSHPVTMSFDKHSRMLKFITQDLELIGSSNVSDSNTFVVHFEITNDTIYPD
                     KMANFQGTCFVDSVFQGVTNNSIEYRDYCNSLSFIHFNDNKVCETDTVMVSNDSEIPF
                     SNNFVWLLDSDTISTDEYFTFSQLDTGKHSITLIVTSPYCQQTQTVNFHVNSFDKPII
                     AYANQYTYVNHNSGSFQWYLNDSIIPGANNGSYNITQTGWYKVFADGLNGCKAFSDSL
                     FLINCKQAAIMQLSDSTPCPRTPVIATNIGHFKENLIWYLNGNVIAGDSIAYVSGLEP
                     GNNYFNIYLDSANCKGYYFQGLVCKVKNFIIPYLSFNGDTIHCDKTFSYYEWFFNGVS
                     IPNNHVQNLHPDSSGYYQLFVIDSLGCAHFTDSIYYDNALGVIQMQLDTIMCVGDAFF
                     ATNKGNYPYSNNFQWFVDSVFQVADSSLFLGNIPVGNHLISLQVIINNDTIVLSKLLI
                     VYDVPPKPIITLNNNQLMCNDYGEWFRNGVSLFYKSYNFGVNQAGWYQIKVYNNSGCF
                     TFSDSMLVIPCSAYNQVNVLNNVVCNSDTLTAFNSSIYPSLNYWSVDGNFISNDTIIH
                     VAGLVQGNHTITLTTTSPGCVFTSNTTAFIFQQTKPTIIENNNTLKCDINNAIYQWFF
                     NGAAITNAKQKTYTVTQQGWYQVQTINTNNCWGMSDSVFCIVCNVNVDFILSDDSVCS
                     KDTIIAYMTNQSNTSVHWLLDGVNYSYDSLIYLSGLSYGYHWLQLVYDTLNCNMEKWQ
                     IIYFDFDTLKFTPQNNMLSCTYYVASFQWYLNGSAIPNATNNTYHITQSGNYSLSAYT
                     FNNCLIISDTIYVEYCNFVPSFTMSTDPLCKFDTLVCYMNHSPSPFLSWKLNGVFIST
                     DSIIYLTNFALGNNWLSLEYLIGFCFYKDEKNVQVMVATKPVITVLQNLISTIPVNSA
                     SFQWLLNGVNIPNANSSSFVVTQTGYYQVSVLYPNSCSNISDSVYVIVSGIQNNSTNN
                     FMLYPNPANEEINIVVPCTFGENYKIELLEVTGAQIEIIFNGKPTTNELKKKIILKNL
                     SKGVYLIRVNETVKYFIKS"
     gene            complement(15886..16815)
                     /locus_tag="PK736_02560"
     CDS             complement(15886..16815)
                     /locus_tag="PK736_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019671033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HRC32307.1"
                     /translation="MENKKINIAIHGGAGTILPHLLTTEKEKAYRDGLHDAINAGKKI
                     LIDGGTSLDAVSAAVMSLENNTMFNAGKGSVFANNGKHEMDASIMDGKSGLAGAVAGI
                     AGVKNPIALARLVMEQTEHVMLIGNGAEEFARQCKLPFMQEEYFHDQFRYDQWIAIRD
                     SDNVQLDHSIDKKFGTVGAVAIDKYGNLAAATSTGGMTNKKYGRVGDTAIIGAGTYAN
                     DATCAISCTGHGEYFMRHVVAYDIHCLMLYKNMSLVDACNHVLFKTLNADAGEGGLVA
                     MDKDGNAALIFNSAGMYRASLNDAMEIVTEIYK"
     gene            complement(16838..17593)
                     /locus_tag="PK736_02565"
     CDS             complement(16838..17593)
                     /locus_tag="PK736_02565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC32308.1"
                     /translation="MTRISAVIITYNEENNIERCIKSLQQVADEVVVLDSYSTDNTKS
                     ISEKLGARFVSYQFENHIQQKNKAIEFAAHDWVLSLDADEALDDQLIASINEIKNSPQ
                     YFGYKMNRLANYCGKWIHHSGWYPDTKLRLFNKHKGAWGGTNPHDMYMLQDKNEKTGF
                     LKGNILHYTFRTVTEHYAQAKRYSDIGAMAHFKKGKRSNWIMVVFSPIIRFVRDYIFN
                     LGFMDGAEGFTICKISAYTSYLKYKKMLALQNK"
     gene            complement(17612..18271)
                     /locus_tag="PK736_02570"
     CDS             complement(17612..18271)
                     /locus_tag="PK736_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32309.1"
                     /translation="MKHTHFFAQHHYLMCIIIFCIIFSGCKKKETAEEEVAVPVVLAE
                     YHFKANINGTDYVYEIKSGNNTNMLSISDNQNFNGMELFRFGAEMDATSAPANLGYPV
                     VGISFQTLKQLTTDVTDADFFGFLKKGNYNYLNTFVLGDNGVVVQLVDRNGNFFYSTD
                     GPQPAATFNVDIATPKAATIGNVAKVKVRALFNCKVYNAVGGTLDIKDGELVIDIENP
                     Y"
     gene            complement(18289..19518)
                     /locus_tag="PK736_02575"
     CDS             complement(18289..19518)
                     /locus_tag="PK736_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC32310.1"
                     /translation="MKENIRIAVDSIKGQLLRSLLTAGIIAIGIFALVGIFTCIDALQ
                     GSISSGFSNMGANSFTIDNRERGIRMGGGGRRKMKRYVPITYKQAQDFKERFHYPASV
                     SISAMASTISTLKYKKEKTNPNIFVFGGDENYLQNSGLKIGRGRNFNNFDMQTGNNSV
                     IIGSDIAFKLFKTTNPVGKEIKIGVNNYNVIGVLEEKGASFGMSADKNCYIPITNLKE
                     NYASSTTSYSISVMVNNVTSMENAIGEATGYMRSVRSDALGGDNSFQITKSDGLANMV
                     ISTLSNIKYSAVAFAVITLLGAAIGLMNIMLVSVTERTREIGIRKALGATSVKIRLQF
                     LVEAIIICIIGGAAGIVLGVFGGLALAIQMGVGLTMPWLWILMGLIVCIVVGIISGIY
                     PAYKASKLDPIEALRYE"
     gene            19783..20256
                     /locus_tag="PK736_02580"
     CDS             19783..20256
                     /locus_tag="PK736_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRC32311.1"
                     /translation="MFSLIILIIAGIILIVFEIMLLPGLVAGIIGAIMMIIGIVMMYK
                     QHGSIAGHITVVASLTATAGAFAWALRSKSWQRFSLKTVNDGKVIDYNARQIKTDDEG
                     TALSAIRPMGKAVFDNNTYEVQSMNEWIDAGSKIKVIQVLPNKLIVEVVRQKEEV"
     gene            20728..21705
                     /gene="floA"
                     /locus_tag="PK736_02585"
     CDS             20728..21705
                     /gene="floA"
                     /locus_tag="PK736_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455820.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HRC32312.1"
                     /translation="MGSAPVFIFTIIGGIILLFLFLYFVPVNLWITAIFSNVKISIID
                     LITMRLRKVPPSIIVNGLIISTKAGLNITKNELETHYLAGGHLNNVITAMISADKANI
                     PLTFKMATAIDLAGRNVADAVQLSVNPRVIDTPPVSAVAKNGIQLIVRARVTVRTNIS
                     QLVGGAGEETILARVGEGIVTNIGSTHDHKMVLENPDQISKTVLAKGLDAGTAFEILS
                     IDIADIDVGDNIGAKLQQDQAAADLKVAQAKAEERRAMAVAFEQEMKAKAQEARARVI
                     EAEAQIPLAIAEAFKNGNLGIMDYYKMQNIQADTGMRDSLGKTDKDSKK"
     gene            complement(22093..22982)
                     /locus_tag="PK736_02590"
                     /pseudo
     CDS             complement(22093..22982)
                     /locus_tag="PK736_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938197.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            complement(23543..24967)
                     /locus_tag="PK736_02595"
     CDS             complement(23543..24967)
                     /locus_tag="PK736_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC32313.1"
                     /translation="MEIAGRKEEVEILESLLDKNESSFVALYGRRRVGKTFLIRQVYA
                     NSIIFECSGVHGDTMQQQLENFWRTLNETKPKAKSSLPPKTWIEAFFRLRAILDKQKT
                     GKKKVIFLDEISWFETPRSGFLAALDIFWNQYCTKRTDIILVICGSAASWIIEKVINN
                     KGGLHNRITNSILLMPLTLGETKDFFGLKNIKLSLKDIAQIYMCLGGVPYYLKDIKPG
                     ESVAQIVNQLFYKKQAILKNEFENLYAALFSNNTFHEKVVKALSTKNKGLTRNEIIKV
                     AKVKGGGGLTIALQELIQCGFVMPIYPINKTKEDCLYRLIDEYSLFFFKFLKTNNNQT
                     NWSQITASAAFKIWSGYAFENLCFKHSTQIKKALGISGVASNEYSYLLKRKGKDKGFQ
                     IDMIIDRADNCVNVLEAKFYNTEFEVTQAYARELLHKATIFTEKTNLNKNVFITMLSV
                     FGVKKNEHYLSAVTNQLLIDDLFT"
     gene            complement(25407..25913)
                     /locus_tag="PK736_02600"
                     /pseudo
     CDS             complement(25407..25913)
                     /locus_tag="PK736_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013998463.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            complement(26109..29039)
                     /locus_tag="PK736_02605"
     CDS             complement(26109..29039)
                     /locus_tag="PK736_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HRC32314.1"
                     /translation="MIAFAFALLNFANEITAQPIDKKYYDAMQWRMIGPHRGGRTVGA
                     VGVPQQPNDFYIGVNNGGVWKTNDFGRTWKPIFDEQPTGSVGDVVVSPSNPNVIYVGC
                     GEGLQRPDLSVGDGIYKSTDGGKTWKNTGLKDGQQIGGLAIDPKNENRVFAAVLGHPY
                     GANTERGVYRTTDGGAKWERVLYKDENTGAVQVTIDPNNSNIVYADMWANRLAPWENG
                     MWSGKESGLYKSIDGGTTWKKLTAGLPTTDQELGRIGFCIAPTNSNRMYATVDAGKMG
                     GIYRSDDAGETWALMNNDGRLWERGSDFAEVKVDPKNADIVYSADVVVWKSIDGGKTW
                     SAFRGAPGGDDYHRIWINPTDNNIMLLASDQGAIVTVNGGETFSSWYNQPTAQFYHVS
                     CDNDFPYNVYSGQQESGSVGIASRGNDGQISYREWQPVGAEEYGYVVADPLDPNIVYG
                     GKITRFDKRTGQSQNISPEAVRSGKYRFIRTAPVLFNPIDNKTLYYAGNVLFKTLNGG
                     NSWDIISPDLTREQYDIPSCIGIYTKEEMKTMPRRGVIYTVAPSYVDINTIWCGTDDG
                     LIQVTRDGGKTWTNVTPPAIKSWSKISLMDASHTDANTAYAAVNCIRLDDQRPHIYKT
                     IDGGKTWKEIVNGLSNFPINVVKEDHKRKGLLFAGSENAVSVSFDDGENWQSLRLNMP
                     ATSIRDLVIKDDDLVVATHGRSFWILDNITPLRQVTTELIKAKYTLYKPQDTYRVRWC
                     MYLDTPLPQEEPAGQNPPDGAMIDYYLPAKSSLVKLEIVNAKAEVIRTYTSIDTMYKI
                     PDVNIPHYWIRPQQILPSGAGAHRFMWDMHYKPLDVPATYPISAIYMNTAPDVTSPFA
                     MPGNYTVRLTVDGKQTSQPLTIKMDPRVKTNVNDLQTQHDVSVMCYNNAKKCIDALKE
                     IEIKKDTSLATFEKEFPKYQNTFITILSLLQESDMPPTTQMIADAKKAEADFEIVWGK
                     WRKK"
BASE COUNT         8960 a         5313 c         5034 g         9735 t
ORIGIN      
        1 caattaataa ataacaattg ggcatgcata aattttgcga tagcaaaatc aacgtactta
       61 atttgtaata attcgaaaaa taggcagcaa gataagtgtt caacaattaa aatacagaac
      121 accctattac caatcttttt cggttgcatt gagataagcc agtaatgcct gagaaatgcc
      181 tttgaaactc ttggtggttt cttcctgtat ctttttccag tttttatctg ataaacctaa
      241 actatcagac acaggatgaa tactcaacaa gtttccgcct gagccaaagc cactggcttc
      301 agcatcatgc gttacactgg aaaataccaa cctgcttttg ccatctttac aaaaaataga
      361 aataacaaac gatacatgtc ccattggtgt tgtattgcca tttacacctt ggggaataga
      421 aacttgaaaa cttccattgc caattattct accggttttt tcgtttgaaa aaaataccgg
      481 gtttttggtc gaataaaaac tgtaaaccag ccactcactg gcttttgtaa agacttggct
      541 ttggcttagt gatttatttt ctgtcgttaa tacgtcttca taaaaaacgt tatcctgggt
      601 atttgctgtc tttatcgaaa caataaagag aaagcagatg agcatttttt tcataatagt
      661 tggttttaaa gtttgtgtaa atcaacaaca tcattatgaa tttcgagtgt aatttatgtt
      721 tcgttttatg ctgtcaaaaa aaacaatttt tttaaaatta cgaggttttg aaagtgcttt
      781 gagaaggtga catggtaagg cagaaatgaa aactgtgcgt ttgcgaataa atcctgtttt
      841 tatttaaggt tgagtaaaac gaaaaaagcc cttcgtgaga agggcttttg gttgtgggag
      901 ctactgggtt cgaaccagtg accccctgct tgtaaggcag gtgctctgaa ccagctgagc
      961 taagctccca acgcaaatga aaaatgtttc atttttttcg gagcgcaaat atagatgcaa
     1021 ttatttaaca gcaaaataaa tatgaaaatt attttttgtc ccaaaatgtc ctttagagga
     1081 aatcacttat aaatctccgt cacaatttcc atagcatcat tcaaactggc gcggtacatg
     1141 cctgccgaat taaaaataag tgctgcgttt tttatcaatt ataacaaaaa cactttttta
     1201 aatatgtacc cttttgtttt aaacaggtca cggttttttt tagcataaaa aaatctacat
     1261 gtgttttggc caatattgtt ttgttgaaaa aaatagccta taattcaaat ataatgttat
     1321 cattgcatct cgaattgaat cacttaccaa caaaaataaa acactactgc catgaaaaat
     1381 ttcttgacca tcttatttct atttattacg aatagtttgt tttcccaaat tccgattgta
     1441 aatattgaca atacaattgg aggaaccagc acagatggtg ctgtggccat ggttaatact
     1501 tcggatcatt gcatggtagt gcttggttat tcttattcaa atttagggct tgacaaaagt
     1561 gaacaatgtt ttggaagctc tgattattgg atactaaaat tggactcggc acaaaatatt
     1621 atttggcaaa aaacatacgg tggttcaggg tatgacatgc cgtcttgtat tgcaagcaca
     1681 agtgacggag gttacattat tggcggcact tcattatcag gtatagatgg taacaaaacc
     1741 gaaccctcaa aaggcggtta tgattattgg atattgaagt tggatgattt ggggaatatt
     1801 gaatggcaaa aagatattgg tggaaattat aatgattttt taagtggtgt tgttcaagca
     1861 caagatggag gatacttggt tgcgggttat tcaagttcgc caataggtgg tgataaaaca
     1921 gaaagtaatc tcgggcagga agactactgg gttttaaaat ttgacagcat gggaaacgta
     1981 ttatggcaaa atacaattgg aggtacagac attgataagt gtttttctgt ggtcgttgat
     2041 gcagatggag ggttcgtttt gggtggttat tctaaatcgg atatgagtgc agataaatcg
     2101 gaaaactata ttggtggtaa tgatttttgg attgtacaaa ttgattcgat tggtaatatt
     2161 gtatgggaca atactatagg aggaagtcag gaggatattt tcacctccat atgccatact
     2221 aatgataatg gatatttaat tgggggatat tctaattcat ggcctagtgc cgataaaacg
     2281 gaacttcccc aagggcatga tgattactgg gtgttaaaac tagacaacag cggaaacgta
     2341 ttatgggaca aaacaattgg cagtgcaggt tatgataatt tgaattgtat tgtaactgca
     2401 cccaacaatc aatatttact tgccggaagt tccgaaatcg gttcttcggg agataaaaca
     2461 gaaattggtt atggttcaac agatgggtgg atggttttga ttgatagttt gggaaattta
     2521 acagaacaaa tagatgttgg tggacaaagt acggataatt tgcatagtgt aatttatgat
     2581 tatcaatcta atttgatagc aggttttgaa tctttttcgg gaataagtgg cgataaatcg
     2641 gaagatgcga atagttcaga tatttgggtt gcaaaatttg ccacaaaata taatactatt
     2701 aagggtaatt catatttcga taccaattca aatctggcaa aggatagtaa tgaatattat
     2761 cttcaaaata aaaggattac agaaaaccaa agcggccgat tcagatttac taatgataaa
     2821 gggaactaca agctacatgt tttagataca ggcaatttcg aagttgaacc gccaccaatt
     2881 tcttattgga attcggtacc ggcaaaacaa aatgttgctt tcaatcaatt taatttaacc
     2941 gattcgatga atgattttgt ttatcaacct gctgccatag ccaatgattt gatgattact
     3001 gcatggccga actctgcgtt cagaccaggt tttcaagctt cgtattctat ccactacaaa
     3061 aacataggca ataccatatt gagcccaacc ataacatttt atattgacca acatctttca
     3121 ttttcatcat ctacaattca accctcgttt gtattccccg attctgttgt ttataatttg
     3181 cctgacatat ggccatttca ggagcaaagt ttttttattt atgtaacagt acataatggc
     3241 actccaatga atacattaat tttatcgggt gttactattt atcctttgat taacgatgcc
     3301 gatattgaaa ataatagcgt ttttcttaat tccaccattg aggtatgggt aacaaattca
     3361 tacgacccca ataatgtgct tgtaagcccc gacacacttt atgagtacga attatcccat
     3421 ccagatttta tagattacat tgttaatttt caaaatacag gaaacgacac tgcttttact
     3481 gtaagagtcg aaaatgtttt atctcccatg ctcgatgaaa gtacttttga aattgttgat
     3541 gcttccaaca ctgttacttt agattataat cctgtttcga gattgatgca atatcatttc
     3601 gataatattt tactgcccga tagtaatgtg gatgagcccg caagtcatgg ttatattcac
     3661 tataaaataa agcctcgcga tacgcttcat attggcgatg taataaataa tggtgcggca
     3721 atatatttcg atttcaatac tcctgttatt accgatattg ctaaagtgca tattgtgtca
     3781 ttttgtgata aattaatatc gctcgatgtt tcaaacttta acgcatgtta tggcgatata
     3841 atcacggtac aaaacaattc tatttttcca tacaataatt atacttggac tttggatact
     3901 actgtaattt cgagtaacga atttgtttca ttatctggtt tgtcggttgg cactcacacc
     3961 attcaattac atatcgatac actaacgtgc catgttgatg caacaaaaaa tattattatc
     4021 aatgccttac ctgtttcatc cattgtttcg aatggcgaca cattatcatc gaatgccaat
     4081 tttgtaaatt accaatggtt gaataacgga atagtgttgc aaaattcaac tcagtataca
     4141 catatagtta atcaaaatgg atggtatcaa ttaatggttt ccgatgctaa tggatgcaaa
     4201 gcaatttccg attcatcgta catgtttcat tgtaatggat tgcttgccat gaatgtgccc
     4261 gattctattg tctgtcctga cgaggcattt gcagcttaca atacatctca aactgaatat
     4321 atacttgatt acgaatggaa aatagacggc aattttttat cgtccgatac tgcaatagtt
     4381 gtgccggcac aaaatattgg caatcaccaa ctctctttaa atatagatac attaaattgc
     4441 acaagcacaa ttgttcaaaa tataaatgta cttcaacctg ttattcctac ttttacattt
     4501 aacggaatag ccatagtttg tgatagtgga tttaatggtt ttcaatggat attcaatggc
     4561 actccaataa caagcgcaaa ccaaataaca tttaatttat tgctaagtgg ttggtatagc
     4621 attgctgcta tcgattcgaa cggttgcaca gaagtatctg attccattta tattgatttg
     4681 tcggcatcta tggctgagta taataatcaa gaaataacat tatttccaaa tccttcaaat
     4741 gatattttgc acgttaaatt tgataaatca ttcgaaaata cttttgcaac tataaacgat
     4801 atgtttggca acgttttaat atcgatacaa ttgaaaggta cagatacccc gatagatgta
     4861 tctttattga gcaatggaat atacgtgatg gttgttcaac gaacgaactc ggtattgcat
     4921 aaatgtttta tcaataaaaa ataaaattaa ttttaaagca catttttatt ttgtttcaat
     4981 tttcatatcg gcaacgcacc atgctaaata gatttatttt tatttcgagt ttgctctttt
     5041 tctactcgac atttttaaag tcacagcctt tgcgtagtga gtggcaatcg gacgttggca
     5101 gttggggtaa cgaatacttt gccgatatgc aagctacctc tgatggggga agtatagcaa
     5161 taggaaccac ctgtaatggg gcttcggcta atgtgtcaat ttactatgga aacaatgatt
     5221 attggttggt aaaatatgat agtgcagggc aactgcaatg ggagaaaaca tatggcggat
     5281 attgggatga caatgctaca agtattatta ccacaagcga tgggggttat ttaattggag
     5341 gctattcaag ctctcctata ggtggcaaca aaacagttgc ctctcatcaa attttcgatt
     5401 attgggtttt gaaattaaat gccaatggca atatacaatg gcaatttact tgtggtacaa
     5461 ccgactttga ttatttgagt tcattacttc aaacaaacga tggcggttac ttattagcag
     5521 gcacttccgt aaccctatct tcgttgcaaa aaccttttgc aggatatggt gatatggatt
     5581 attggataat aaagctcgat tccaatggtg tgaaactttg ggataaatgc tatggtggtg
     5641 ccgatgagga tttatgtaca agtatagtaa aaggtaagga tggtaatgtt ctgcttggcg
     5701 gtatttcgaa atcatatatt ggtggaaata aaaccgaaca tgccaaaggc ttacacgatt
     5761 attggatagt aaaaataaat tcgacaggcg acattatttg gcaaaatacg attggggcga
     5821 gccaggaaga ttatttgtat tcgctttact caacagaaga taatggtttt gttttgggag
     5881 gcaattccaa ttcatccatc accaacgata agacagaaaa caaaattgga cttcaggatt
     5941 tttggattat caaagttgat tctattggaa gcatacaatg gcaaaacact attggaggta
     6001 cccaagatga gttcattggt aatattattc aaaataaact tggtggatat acgcttgttg
     6061 gaatttctgc atcaccgaag ggtggagata aaaaaacaat aaattactta gtagataatt
     6121 ggatagtgtt tttggattct ttaggaaata tcatcgaaca aaaaacagtg tcaactttag
     6181 gtaatgaggg tgataaaata aatctgaatt attattataa aacttatttt attaatgcaa
     6241 gctgcaaaat cattaatacc tatgatggaa attatatttt aggcacaaca agcaattcca
     6301 atatttcact ttccaaagca gctaaactga ttgaccctac tggcaataaa aattttgatt
     6361 tttggttaca aaaatttagc agcaattata atcttgccac cggaaaatgt tttgttgatg
     6421 tgaataaaaa taatgtcttc gatatcaatg aaattcctat tcaatcaatg cctgtttcga
     6481 aaggtaccgg tactacggtt gtgtacactg atacaaatgg caattacgaa ttgccgtatt
     6541 ttcaaaccgg aaattataca tataaagcac caaaaactta taacagcctt acaccaacac
     6601 caacaaaata taccgttgct ttttcttctg catctagtat agattcagtg aataattttg
     6661 cgtataaatc cactgcacca ttcaacgact tagaagtttc gcttgaacct ttaggtataa
     6721 agggtgctgg tgacaccact tattatatta tatcgtataa aaataatggc actgcatctc
     6781 aacaacctac acttgtcttt tataccgatt ataactttgt ttttatttcg tcatccatta
     6841 ctcctgatac cataacaacc gattcgttga tttggaattt atctactttg ccttctttaa
     6901 gtaaaggaca tttatttttg aaaatttatg aaaagcctaa taataattac ttgccatccc
     6961 aaacaaaagt tgccattttc ccaatagcca atgatgttat agattcgaac aatgtggcaa
     7021 tatggggccc actagtaaat aatactgtaa gtgattttta tacgactcaa tcaaaaaacc
     7081 aacatttttt tgtaacaaca aatgataaat tcacaacaga ctttgcgtac agttactgtc
     7141 atacaaaaaa tgacacatgt aaacaattaa tgttcgaagt aataattcct ttagggatag
     7201 atttttctac tttccgtgtt gtatcatcta cgaacccaat tgaaataagt tacgataaac
     7261 catcgcgatt tttaaaaatt attgccaaca atttattatt atacaaaagc aatactattt
     7321 ccgacaccaa tgttttttgc ctacacttcc aactcacaag tgactctatt atagatacta
     7381 tcgttgtaag ccaaaatata tttttcgtag attcattgtt taaatttgaa aataaaacaa
     7441 aaattatttt tagccaatat tgcgataacc ttgcaaaaat catttttgat gatagtaacg
     7501 tttgcgaaac agataccgta aaagttaata acatcgcaag cataccgttt tcaaataatt
     7561 ttgtttgggt gctcgatagc gatacagttt cgaccaatga cttttttatt ttttataact
     7621 tagttcccgg caaccacaat gttactttaa ttgtaaattc tccttattgc caaaaaatag
     7681 ttagccatgc tttttcagtt aattctttta gttcttcgtt taagcctcca atattcgcgg
     7741 atttggggca agttagaagc tggggcaatc ctgacccacg cgacccattt caatggtatt
     7801 ttaacggaac tccagtggga accaattacc aaagttgcca aatggctcaa gcgggatggt
     7861 atcaagtgtt tgccaataat atttatggat gtcagtcttt ttctgattct ctctttgcaa
     7921 taaattgttt tgatgcaatg gatatacagt tatccgattt aacaccttgc cctcatactc
     7981 cattatttgt aagcaataat ggcaatttca aagtaagtgc taaatggtgg ttggatttaa
     8041 aaccaattcc gggagattca ggtttgaata ttacatcttt ggaacctggt attcataaaa
     8101 tacttttaaa aattgatagt gcaaattgta aactttatga tgaaaataaa tctttctggg
     8161 ttaacgaatt tatcattcca tatttagtat ttgtaaatga tacaatactt tgcgataaaa
     8221 cattctctta ttacgaatgg ttttttaatg gcgtttcgat tcctaataac aatgttcaaa
     8281 atttacatcc tgattcgtct ggttattatc aattattcgt tatcgattct ttaggatgtt
     8341 cacattttac cgattccatt tattatcata atgccttagg tgtaatccat ttgcaattag
     8401 ataaaacgat atgtgaaggt gatgcttttt ttgccaccaa tcaaggcaat tacccataca
     8461 ataataattt taattggttt gtcgattcta tttttcaagt caccgattct gcgctttttc
     8521 ttggcaattt gccggcagga aatcacaaaa taacattgat ggtgattgat tatgttgata
     8581 cagtactttt gtctaaattt atcaatgtta atgccctgcc ggtaaagcct gttattgcat
     8641 ttgctaataa tgtcgtttcg tgcaatggca gttacaacgt ttataaatgg tatcgtaatg
     8701 acactttggt gacctataac tcaagcatga ctatctttca agcaggatgg tataaactta
     8761 gagtggaaaa tacttcaaat tgttttattt attccgatag tgtttttctg ctcccatgta
     8821 atttatattt atctcttgaa gttgttaatt cggggttttg cattggcgat actttaaaag
     8881 catataacaa aagtattttt ccttctacaa attactggac cttagatgga aattttattt
     8941 cttcggatac aagtataagc attagtggta ttagcgtggg gtggcatacc cttgtgttag
     9001 aggtcacagc aaatggttgc acaactacaa cttcagctag tataatagtt tcgcaacccg
     9061 taatacctct catcaataaa ttctacaatg atttacattg caatttaagc agtttttctt
     9121 atttgtggta tctcaacggc aatcctattt cgaacagtaa tgtacaacat tggataattg
     9181 ctgcctcggg ttggtataaa gtgagttgca ccaatctata ctcatgcacc tctttttccg
     9241 actcaatatt ctgtaatctt tgccctccaa tggttaattt taatgtttac aacgataatg
     9301 tatgttttac agatacaatt attgcatatt ccattacacc aatgtaccca tcaacatatt
     9361 ggtcaattga tggtgttttt tattcgagtg attctttaat taaaatacac aatgtgggtg
     9421 cgggtattca taatcttcga ttaaattccg actctgcaaa ttgtgtaact tcgcattacc
     9481 gtaacattac ttttgcagtt gattcagcca gatttgtggc ccaaaataat ttgcttacca
     9541 gcacttataa tttattgtct tatcaatggt atttaaatgg tttgcctatt gccaatgcca
     9601 ccaataaaac tttaatcatt taccaaagtg gttattattc cctatctgga atggcaaacc
     9661 ttaattgttt tgttttctct gatactcttt atataccact ctgcaattat actccaacat
     9721 tcacaatgtc gtcaacatcg gtgtgtatgt acgatacagt tgtctgctat atgaatcaat
     9781 cgccttcgac atataaaagt tggatgctaa atggaacttt catatcgcag gattctgcga
     9841 ttaatatttt caattttccc ccaaacacaa attttatttc attagtatat tctacaaatt
     9901 tttgttatag acttacaacg caaaatttcc agggagtaac ggccatcaaa ccaaccataa
     9961 cagtttttac caatcaattg cagacaaaca atgtggcttt tgcaaatttc caatggttgc
    10021 taaatggtgc gttaataccc ttggccacaa catacattta tactcctaat caatcaggtt
    10081 actatcaggt agatgtaact tatcctaatg gatgcagcaa tatttccgac tctgtttatt
    10141 ttggtttaaa tggtttcgaa aattatcata caaaccattt cagtatttat ccaagtccgg
    10201 ccgatgaaaa catacaaata catttattta ctaatgcaga aaatacaaat gcaattttgt
    10261 tagataaaca tggccgaata gtattgagta agaaactcga aaataaaatt tctactttcg
    10321 aaacaaacga aataagtcag ggagtttata cattgataat cgaacaaaat aatgcgcgcg
    10381 aatttcaaag aattgtgatt gtacacaatg ctgataatga aagtaaataa tcatgagcat
    10441 aaagtcaaaa tacctaacaa taaaaaaaat agcgctgatt gcattatgtc tttttgcaat
    10501 tgattgtagt atttctttcg gtcaatctct acgttacgag tggcaagtgg atctcggatg
    10561 taacaatgat gattattttt ctgatttaga aaaaactcct gatggtggct ctattgtgtt
    10621 gggtacttca tttggaggaa atacgggtaa taaaactgtt tattatggtg atggcgatta
    10681 ctggcttgca aaactcgata gtacggggca attgcagtgg caaaaaactt atggtagcga
    10741 ttcttatgaa gtagccgcca gcgtaacaac cacaaccgat ggaggataca ttatttgtgg
    10801 tttctctgca tctgattggg gtggtaataa aaccgctacg ttatatggat attatgatta
    10861 ctgggttttg aaattaaatg ccgcaggcta tatagaatgg caaaaatctt ttggtactac
    10921 tgatggcgac tttgcaattg atttggttca aacaaatgat ggtggtttta ttattggtgg
    10981 aacctcagat acacaaacat catccaataa attatttgca ggatatggcc ttaaagatta
    11041 ttggattgta aagctcgatt cgaatgggac aaaactttgg gataagtgtt atggcggtgc
    11101 ggactacgat gtacttactt gcttaataaa aagtgataat gggaatttgc ttttgggagg
    11161 atattcaaaa tcatacattg gaggaaacaa aacagaacat gccaaaggat taaatgatta
    11221 ctggataatt aaaattgatt ccataggaaa cattatttgg caaaatacaa ttggtggcag
    11281 tcaagaagat tttctttttt cggcttttcg cacaagtgac aatggttttg tgttgggcgg
    11341 aaattccaat tcatctatca ctacggataa gacagaaaat aaaattggac ttcaggattt
    11401 ttggattgtc aaggttgatt cgatgggaaa tgtgcaatgg caaaatacta ttggaggtac
    11461 taaagatgaa aaaataggtg atgtaattca aacaagtaat ggagaatatg ttgtggctgg
    11521 taaaacggtt tctgagacgg ggggtgacag acaatcaatc aattatggtt cagacatttg
    11581 gcttttgttt ttggataccg taggaattat tgtggatcaa aaaattatat gcgctaacaa
    11641 agatgatggg gaaagctaca tttgctgtta ccaagaatta ccaaattgta aattagtaga
    11701 atctaatgat ggaggtcttt ttttaggaac ctcaacacgg tcaggtattt cgctggagaa
    11761 aaatgcagca ctcactgttt atggtaatta ttatatcgat ttttcagatt attggataat
    11821 aaaattaaac aaaaactata atttagcaaa tggcaaatgt tacctggacg tgaataagaa
    11881 caatgtattt gatggtaatg ataaatcaat aaatggattt gctgttaata acaatggtag
    11941 tcaagtagtt tttactgata atgatggcaa ttatgagttg acttattttg accctggtac
    12001 ttatacttac aaacctcctt taaaatataa tggcataagt cccacaccgg caaagcttat
    12061 tgttagtttt acaaatacat caacaataga ttcgctgaat aattttagct acaagcctcc
    12121 cgcaacatta caggatttag aaatttctct tacaccggca ttgttaagct attatcccga
    12181 tacagaatat tttagaatcg agtatcagaa caatggaaca tcgggccaac tgccaattat
    12241 tgttttctac accgatacaa attttgtttt catttctgct tccattgcat ctgattctat
    12301 tactcctgat tctgttattt ggaatttgcc tttactgcct ccaaatagca gtggtcattt
    12361 ttacattaaa gtttacaaaa aaaccaaaag ttatgacaat ccatttataa caaaggttgt
    12421 gattttgcca attattggcg atgacataga tactaacaat atagatatat ggaacgcaat
    12481 gaatagtaat tatgatcaag gatttaggtg tacccaatca gcaactaaat attatttacg
    12541 ttaccttggc gataagttta caaccgattt tacttatagc tactttaagc agaaaattga
    12601 cacttgcaat caaattatgt tcgaagttat tatacctacg tatttaagtt tttctacttt
    12661 taaattggta tcctcctcac atccggtaac aatgagtttc gacaaacatt cgcgaatgct
    12721 taaatttatt acgcaagatt tggaattaat agggagttca aatgtttccg attctaatac
    12781 ttttgtcgtt cacttcgaga ttacaaatga tacaatttat ccggataaaa tggcaaactt
    12841 tcaaggaaca tgttttgtgg attccgtttt tcaaggtgta actaataatt ctattgaata
    12901 tcgtgattac tgcaatagct tatcatttat tcattttaac gataataaag tttgcgaaac
    12961 ggatacagta atggttagta atgactctga aattccattt tccaataatt ttgtttggtt
    13021 gctagatagt gatacaattt ccactgatga gtattttaca tttagccaat tggatacagg
    13081 taaacactcc atcactttga ttgttacttc accttattgc cagcaaacgc aaacagtaaa
    13141 ttttcatgtc aattcattcg ataaacctat tattgcttac gcaaatcaat acacttacgt
    13201 taaccataac agcggctcat ttcagtggta tttgaatgat tcgataattc ctggcgctaa
    13261 taatggcagt tataatatca cccaaacggg ttggtacaaa gtattcgctg atggattaaa
    13321 tggttgcaaa gctttttcgg actctttgtt tttaataaat tgtaaacaag ctgcaataat
    13381 gcagctttcg gattctacac cttgcccgcg cactcccgtc atagctacaa acattggaca
    13441 ctttaaagag aatttaattt ggtatttaaa tggtaatgtg attgccggag attcaatagc
    13501 atatgtttct ggccttgaac ctggcaacaa ttattttaat atttatttag actctgctaa
    13561 ttgtaaaggc tattattttc aagggctcgt atgtaaagtc aaaaatttca ttatcccata
    13621 tttatccttt aatggtgata caatacattg cgataaaaca ttctcctatt acgaatggtt
    13681 tttcaacggt gtttcgattc ctaataacca cgttcaaaat ttacatcccg attcttccgg
    13741 ttactatcaa ttattcgtta tcgattcttt gggatgtgct cattttaccg attccattta
    13801 ttacgataat gccttaggcg taatccaaat gcaactggat actataatgt gcgtaggcga
    13861 tgcttttttt gccaccaata aaggaaacta cccatacagt aataattttc agtggtttgt
    13921 cgattctgtt tttcaagttg ccgattcttc actttttctt ggaaacatac ctgtgggcaa
    13981 ccacctcatt tcgttgcagg taattattaa taacgatacc attgttttgt caaaattgct
    14041 tatagtttat gatgtgcctc cgaagccgat aatcacactt aacaacaatc aactcatgtg
    14101 taatgattac ggtgaatggt ttcgcaatgg tgtatcatta ttttataaat cttataattt
    14161 tggtgttaat caggcaggct ggtatcaaat aaaagtttat aataactcgg gatgttttac
    14221 tttttcggat agtatgttgg ttattccttg cagtgcctac aaccaagtaa atgtactaaa
    14281 taatgttgtg tgtaattctg atacattaac cgcattcaat tcaagtattt atccttcttt
    14341 aaattattgg agtgtagatg gtaactttat ttctaatgat accattattc atgttgctgg
    14401 cttggtgcaa ggaaatcata ccattacttt aacaaccact tctcccggat gtgtatttac
    14461 ttcaaataca acagctttta tatttcaaca aacaaagcct acaatcattg aaaataataa
    14521 cacactaaaa tgtgatatta acaatgcaat atatcagtgg ttttttaatg gggctgcaat
    14581 aacaaatgct aaacaaaaaa catacaccgt tactcagcaa ggttggtacc aggttcaaac
    14641 gataaataca aataattgct ggggaatgtc cgattctgtt ttttgtatag tatgtaatgt
    14701 aaatgttgat tttattttga gcgatgattc agtttgtagt aaagacacca taattgcgta
    14761 catgactaat caatctaata cttcggttca ttggttgctt gatggtgtaa attattctta
    14821 cgattcgtta atttatcttt caggactttc gtatggttac cattggttac agttagtata
    14881 tgatacactc aattgcaata tggaaaaatg gcaaatcatt tattttgatt ttgacacatt
    14941 aaaatttacg ccccaaaata atatgttaag ttgtacatat tatgttgcat cttttcaatg
    15001 gtatcttaat gggagtgcta tacccaatgc cacaaacaac acgtaccaca taactcaaag
    15061 cgggaactat tctctttcag cttatacatt taataattgc cttattattt ccgacactat
    15121 atatgttgag tattgcaact ttgttccttc tttcacaatg tcgacagacc ctttatgtaa
    15181 atttgatacc cttgtatgtt acatgaatca ttcaccatca ccgtttcttt cctggaaact
    15241 taatggtgtt tttatttcta ccgattctat tatctacctt actaattttg ctttaggtaa
    15301 taattggctt tcattagagt acttaattgg tttttgtttt tataaggatg aaaaaaatgt
    15361 gcaagttatg gttgctacca aaccggtaat tacagtattg caaaatttaa tttcaaccat
    15421 tcctgtaaat tcagcttctt ttcaatggtt gttgaatggt gtaaatattc ctaatgccaa
    15481 ttcaagcagt tttgttgtaa cacaaactgg atactatcag gtttctgttt tatatccaaa
    15541 cagttgtagt aatatttccg actctgttta tgttatcgtt agtggaattc aaaataacag
    15601 cacaaataat tttatgcttt atcctaatcc ggcaaatgaa gaaataaaca ttgtagtgcc
    15661 ttgcacattt ggcgaaaatt ataaaattga attgcttgaa gtaacgggag cccaaattga
    15721 aattattttc aatggcaaac caactaccaa cgagttgaag aaaaaaatta ttttgaaaaa
    15781 tttatcaaaa ggtgtttact taatacgggt aaatgaaacg gtgaaatatt ttattaagag
    15841 ttagccgtgt gatatttttg ccatcagtga taatggcttt ttctctcact tataaatctc
    15901 cgtcacaatt tccatagcat cattcaagct tgcgcggtac atgcctgccg aattaaaaat
    15961 aagtgcggca tttccatctt tgtccatagc taccaaaccg ccttcgcctg catcggcatt
    16021 taaggtttta aataatacat ggttacatgc atctaccagc gacatgtttt tgtatagcat
    16081 gaggcaatgt atatcgtatg ctaccacatg tcgcataaaa tattcgccat gccctgtaca
    16141 tgatatagca catgttgcat cgttggcata tgtacccgca cctataattg cagtatcgcc
    16201 tacgcggcca tattttttat tggtcatgcc tcctgtgctt gtagcggcag caaggttgcc
    16261 gtacttgtct attgctacag cgcccacggt gccaaatttt ttatctatac tatggtcaag
    16321 ttgcacgtta tcgctatcgc gaattgctat ccattgatca tagcgaaatt gatcgtgaaa
    16381 atattcttct tgcataaatg gtaacttaca ttgccgtgca aattcttcgg caccatttcc
    16441 tataagcatc acgtgttcgg tttgttccat tacaagacgg gcaagggcta taggattttt
    16501 tactcctgct attccggcaa cggcaccggc aagcccgctt ttgccatcca ttatactggc
    16561 atccatttcg tgtttcccat tattggcaaa caccgaaccc ttaccggcat tgaacatcgt
    16621 attattctcg agcgacatca ccgctgcgct cactgcatcg agcgaagtgc cgccatctat
    16681 caatattttt ttcccggcat taatagcatc atgcaatcca tcgcgatagg ctttttcttt
    16741 ttcggttgtg agcaggtgag gtaaaatagt tcctgcacca ccatgaatag caatatttat
    16801 ttttttgttt tccatcgtat actgttatag ttatatttta tttattttgc agggcaagca
    16861 tttttttgta tttcaaataa gatgtgtatg ccgatatttt gcaaatggta aatccttcgg
    16921 caccgtccat aaaaccaaga ttgaaaatat agtcgcgcac aaatcttata atggggctaa
    16981 acacaaccat aatccaattg ctgcgttttc cttttttaaa atgcgccata gcaccaatat
    17041 ccgaataacg ttttgcttgg gcatagtgtt cggtaactgt gcggaaagta taatgcaaaa
    17101 tattgccctt caagaaacct gttttttcgt ttttatcttg cagcatatac atatcatgag
    17161 gattggtgcc tccccacgct cctttgtgtt tattgaataa tcgcaatttt gtatcgggat
    17221 accaaccgct gtggtgtatc cacttgccgc aatagttggc cagccggttc attttataac
    17281 caaaatattg tggcgagttt tttatttcgt ttatcgatgc aatgagttga tcatccaggg
    17341 cttcatcggc atctaaactc aacacccaat cgtgtgcggc aaactctata gctttgtttt
    17401 tttgctgtat atgattttcg aattggtacg aaacaaatcg ggcaccaagt ttttcgctaa
    17461 ttgattttgt gttatcggta gagtaggagt cgagcaccac cacttcatcg gctacctgtt
    17521 gtaatgattt tatacaccgt tcaatattat tttcttcgtt gtacgtaatt atcactgccg
    17581 aaattcttgt cataaatttt acaaaaaaaa attaataagg gttttcgata tcgataacca
    17641 attcgccatc tttaatatcg agtgtgccac ccacagcatt gtatactttg caattgaaca
    17701 atgcacgcac ttttactttg gctacgttac caatagtagc tgccttgggt gtggcaatat
    17761 caacattgaa agttgctgca ggttgtggtc catcggtgga ataaaagaaa tttccgtttc
    17821 tatccaccag ttgtacaaca acaccattat cgccaagcac aaaagtgttg agataattat
    17881 aattaccctt tttgaggaag ccaaaaaaat cggcatcggt aacatctgtg gtcaattgtt
    17941 ttaatgtttg aaacgaaatg ccaacaacag gataacccaa atttgccggt gccgatgttg
    18001 catccatttc ggcaccaaag cggaaaagtt ccatgccatt aaaattttga ttatcgctta
    18061 tcgacaacat attggtattg ttgccgcttt ttatttcata cacataatcg gtaccattta
    18121 tatttgcttt aaaatgatat tcggcaagaa caaccggaac tgctacttct tcttcggctg
    18181 tctctttctt tttacatcct gaaaaaataa tgcaaaagat tatgatacac attaaataat
    18241 gatgctgcgc aaaaaagtga gtgtgtttca tttcaaaaat tattttagtt attcataacg
    18301 taacgcttca ataggatcga gtttcgatgc cttgtatgca ggatatattc ccgaaattat
    18361 tcctacaact atacaaacaa ttaatcccat caatatccaa agccacggca ttgttaagcc
    18421 aacacccatt tgtatggcaa gtgccaaacc gccaaacacg ccaagtacaa tacctgcagc
    18481 accaccaatg atacaaatga taatggcttc aaccaaaaat tgcaaacgta tttttaccga
    18541 tgtagcacca agtgctttgc gaattccaat ttcacgcgta cgctcggtta ccgatacaag
    18601 cattatattc attaaaccaa tggctgcacc aagtaatgta attactgcaa aagccactgc
    18661 cgaatatttt atattactta atgtagagat taccatattg gcaagtccat cgctttttgt
    18721 aatttgaaac gaattgtcac caccaagcgc atcgctgcgc acactgcgca tataacctgt
    18781 ggcttcgcca atggcatttt ccatacttgt tacattattt accatcaccg atattgaata
    18841 ggatgtagtg gatgatgcat aattctcttt caaattggta attggtatgt aacaattttt
    18901 atcggcactc ataccaaacg aagcgccctt ttcctcgagc acaccaatca cattgtaatt
    18961 gtttacacca atttttattt cttttcctac agggttggtg gttttgaaaa gtttgaatgc
    19021 aatgtcgctg ccaataataa cactgttgtt gcccgtttgc atatcgaaat tattaaaatt
    19081 gcgaccacgg ccaattttca atccggaatt ttgaaggtag ttttcgtcac cgccaaacac
    19141 aaaaatattc ggattcgttt tttctttttt atattttaat gttgaaattg tagaggccat
    19201 tgccgaaata gaaacactgg ccggataatg aaaacgttct ttaaaatctt gcgcctgctt
    19261 atatgttata ggaacatagc gtttcatttt tctgcggcct ccaccgccca tgcgtatacc
    19321 acgctcgcga ttatcgatgg taaacgaatt tgcacccatg ttcgaaaacc ctgaactgat
    19381 gctgccttgc aaggcatcta tacaggtaaa aattcccacc aatgcaaaaa taccaatagc
    19441 aataatgccc gcagtaagca acgaacgtaa tagctgtcct tttatactat ctaccgctat
    19501 gcggatgttt tctttcattg atttttgttt gcaaatgcga attaagcgaa ttaattttta
    19561 gcacctctca cactggcaaa taataagact acatttcacg caatttgttt cattaatatt
    19621 gaaacatcat tcgtcttaaa ataaactgcc ttcataaact attctactct atattttctt
    19681 tcattttaag tttgaaagta tttattgaaa agttgagtgt gccaccagaa gaaacattgt
    19741 aataaaattt cttacttttg ggcactctca aaatatttat tcatgttttc tctcatcatt
    19801 ctcatcattg ccggtataat attaattgtg ttcgaaatta tgctcttgcc gggtttagtg
    19861 gctggcatta ttggtgccat aatgatgata attggaattg taatgatgta taagcaacat
    19921 gggagtatag caggacatat tacagttgta gcttcattaa ccgcaacggc aggggcattc
    19981 gcctgggcat tgcgcagcaa gtcatggcaa cgttttagtt taaaaaccgt aaatgatggt
    20041 aaagttatag attacaatgc caggcaaata aaaaccgatg atgaaggcac tgccctatcg
    20101 gcaatacgac caatgggcaa ggcagtattt gataacaaca cctacgaagt gcaaagtatg
    20161 aatgaatgga tagatgccgg ctcaaaaata aaagtgatac aagtgctgcc caataaattg
    20221 attgtggagg tagtaaggca aaaggaagaa gtttaaagtt caaggtttaa agttcaaagc
    20281 caaaagagat aagtcaaaag ttcaaggttt taatttcaaa gtcaaaagtg aaaaaattaa
    20341 ttaccattgt tgtgcaaata aaaccatgag accctagttt aatatttgtt tcttcgcact
    20401 cgaaaataag aaatgtaatt agtgacaaat aaaaagactc accaaaaaca aaactgagta
    20461 caaggcacac agcaaacgaa aactaagaac taattaacga agaacaaaga acaaggctca
    20521 cagcaaacgt aacgaagaac taagaacaag cctcgcagca aacgtaacaa agaacaagcc
    20581 tcacagcaaa cgtaacgaag aactaattaa cgaagaacga agaacaaggc acacagcaaa
    20641 cgtaacgaag aacgaagaac aagtctcacc gcaaacgtaa ctaagaacta aaaagaataa
    20701 caaattaact aataacaaat aactaaaatg ggttcagcac cggtatttat ctttacaata
    20761 ataggaggaa ttattcttct gtttttgttt ctctattttg ttccagtaaa cttatggatc
    20821 accgcaattt tttcgaatgt aaaaattagc attatcgatt tgataacaat gcgattgcgc
    20881 aaagtgccgc catcgataat tgtaaatgga ttaatcatca gcaccaaggc aggattaaac
    20941 attactaaaa atgaactcga aacacattac ctggcaggtg gtcatttgaa caatgtaatc
    21001 acggcaatga taagtgccga caaggcaaac attccgctta catttaaaat ggcaacggct
    21061 atcgaccttg cggggcgtaa tgtggccgat gccgttcagc taagtgtaaa tccccgtgtg
    21121 attgatacac cgcctgtaag tgctgttgca aaaaatggta ttcaacttat agtgcgcgcg
    21181 cgtgttacag tgcgtaccaa catcagccaa cttgtaggag gtgccggtga agaaactatt
    21241 cttgcacgtg taggcgaggg tattgttaca aacattggtt ccacacacga ccataaaatg
    21301 gtattagaaa atcccgatca aatatctaaa actgttttgg caaaaggcct cgatgccggt
    21361 actgctttcg aaattctttc aattgatatt gccgatatag atgtaggcga taacataggt
    21421 gccaagttac aacaagacca ggccgcagcc gacttaaaag tagcacaagc aaaagcagaa
    21481 gagcgcagag caatggctgt ggcattcgaa caagaaatga aagccaaagc acaggaagcg
    21541 cgtgcacgtg tgatagaagc cgaagcacaa attccattag caattgccga agcatttaaa
    21601 aatggtaacc ttggcattat ggattattac aaaatgcaaa acatacaagc ggatacaggc
    21661 atgcgcgatt cacttggcaa aacagataaa gacagcaaaa aataattttg atttcgtaca
    21721 aaaaaaatcc acttcatacg aggtggattt tttttgctca caacttttta gttcgtatta
    21781 atttttgaat ttgcaatgct taggaacgaa gcatctctcc tgcaaaagct atttagtgca
    21841 aagcaatcgt gatctaaaaa acacaaagca ccctttaatt ttaccgataa tgctccacca
    21901 ccacagtcat tcaggaggaa ccctatttct aaaaccaaaa attttgttcg ataaaaaaat
    21961 tatttcttct tccattgcaa cttcaccatc tcaaatctgt ttcagatttt tttgcatcgg
    22021 aaattatttg agaaatcaaa tacacattga tttcgcgcaa taaagaatga ggctcattaa
    22081 aaaaatgtgt gattattttt ttctgctgat ttgttttttc ttttctgccg gaggcaaaaa
    22141 gttttcgcct gttacaactt tttttcctgt tttattttcc agcgcaagct ttgcatcttt
    22201 agcaatcttg ccgccttttt ttcctgcaac agcattttgc tttacgcctt tggctttttc
    22261 actttcagca atttgtcggg tcgaaagttc cgccaatgca gtaaaaatta attcggcttc
    22321 gctcatatgg tcgcgaaggt tttgtgtttt taatccttta agacttttat gtttttgaac
    22381 agatatcccc gtccattctt gataggcctc atccggcttt cagccacctt ctccaaaaga
    22441 gaagggaaac ggcaaaaata tttgtttgtt aaaaatacaa actcatctcc tttttgcacg
    22501 ccactttctt gccagtaatc ggttaattta tttcgggttt cttgtcctgt cattcgttgc
    22561 tgtatccact tttcgctacg acctaattga tgccagtttt ctcttgcacg gtcgaggctt
    22621 tgttccgggt ccgatatttc tttcattctt tcgtaaccgg tttttgcaag ccactgctta
    22681 aatggttctg ccttcggtga cggtatagat tgaataatgc gcagtaattg ttcggtatcg
    22741 gcaacatctg ttgcatactt tttaccatca tcagatttta atttcagttg tccgattttt
    22801 tcggacaact cacttccttc ctttatcagc ttggctttta aatcgctcca gtattttctt
    22861 gggcggtcgg ttccagtgag tatttcaatt acatcgataa ttgaaaaata ccatttctcc
    22921 tctttttcat cccaatgtgt tcttactttt ttttgttcaa ataattttat gctattgctc
    22981 attgtgtcaa tttattttcg tttttataat tgctgacaga tcactttctt ttctccaaat
    23041 ataactactt gttttttcat gatgataaat gttggcaact tttgcaaatt aaaatgtaca
    23101 caaatttaca ccaccaccta atgtcattca ggaggaacct tatttctaaa aacgaaattt
    23161 ttgttcaata gcaaaaatta tttcttcctc cacttcctcc acacaatttc aaaatccatt
    23221 tccgcttttt tcgcatcggt tatcatttgt gttgttggtg gcatatcact tttgagcaat
    23281 tgaggacgga actgcaaaaa aaagaagtag aattattttt ttgcaattca attcatttat
    23341 tccggggatt aattttacag cactaactgt atttggaatt tttactaatt tcataaataa
    23401 ttgtaaacat acttttttca actatcaagg ttggggacct tcattagttt tgataaaaca
    23461 ccacctatta aataagccga atacataagc gtatcataaa ttaaattgca tagggctttt
    23521 gtttttattt aattttatat tgttaggtaa acaaatcatc tattaacaat tgattggtta
    23581 ctgccgacaa ataatgttcg ttttttttca ctccaaacac cgaaagcata gttataaaaa
    23641 catttttatt caagtttgtt ttttctgtaa aaattgtggc cttgtgaagt aattcacggg
    23701 cataagcttg ggtaacttca aattcagtat tataaaattt tgcttccaaa acatttacac
    23761 agttatcggc acggtcaata atcatatcaa tctgaaagcc tttgtctttg ccctttcgtt
    23821 ttaataaata agaatactcg tttgatgcaa ctccactaat gcctaatgct ttttttattt
    23881 gagtgctgtg tttaaaacaa agattttcaa atgcataacc actccatatt ttaaatgcag
    23941 cacttgctgt aatttgtgac caattggttt gattgttatt ggtttttaaa aatttaaaaa
    24001 agaacaaaga atactcatct attaacctat acaaacaatc ttctttagtt ttattaattg
    24061 gataaattgg cataacgaaa ccacactgta ttaattcttg taatgctatt gttaagcctc
    24121 ctcctccttt tacttttgca actttaataa tttcatttcg tgtgaggcct ttattttttg
    24181 tagatagtgc tttcaccact ttttcgtgaa atgtattatt gctaaaaagc gcagcgtata
    24241 aattttcaaa ctcatttttc aaaatggctt gctttttata aaacaattga ttaactattt
    24301 gtgccacact ttcgccaggc tttatatctt taagataata aggaacacca cccaagcaca
    24361 tatatatttg agcaatatct ttgaggctta atttaatatt ttttaatcca aaaaaatctt
    24421 tggtttcgcc caaggttaag ggcattagta aaatactatt ggttatgcgg ttgtgcaatc
    24481 ctcctttgtt atttattact ttttcaatta tccaagaagc tgccgaacca caaataacta
    24541 aaataatatc agtgcgtttg gtgcaatatt gattccaaaa aatatctagg gcagctaaaa
    24601 agccagaacg tggggtttca aaccatgaaa tttcgtctaa aaatattact ttctttttgc
    24661 ctgttttttg tttatctaaa atggccctca accgaaaaaa cgcctcaatc catgttttgg
    24721 gtggtaatga agatttggct tttggctttg tttcgtttaa cgttctccaa aagttttcta
    24781 attgttgttg catcgtatcg ccatgcacgc cactacattc aaaaattatt gaattggcat
    24841 aaacctgacg tattaaaaat gttttgccaa cgcgccttct gccatacaaa gcaacaaatg
    24901 aagattcatt tttgtcaagt aagctttcta atatttcaac ctcttctttt ctgccagcaa
    24961 tctccatttc tctattattt ataacttgcc aaatgtagcc taaaaagtat gaatttggca
    25021 agttataaat aaatataatt tactaaattg tttgctatat atgatgtttt gaaaagacgc
    25081 aagaaattat aatttgccaa atcacgatta aaaagcacta atttggcaag ttataaaagc
    25141 acttgaattt aaactgttgt ttttttattt tagatattta gctacttgta aaagcgaatt
    25201 actgctatgg cccagcttgt agtttggttg ccctatgatg gaattttata gacggggttg
    25261 agtaaatttt gaggttgcaa atttttataa tatttaaatt tatggacggt tagcctgcca
    25321 attccttatg gatttcatcc gactttcaac caacttctct gaaggaaaaa ggaattggaa
    25381 taaatatttt tttgttagtg agaaaatgca aactcattac ctttttgcac gccactttct
    25441 tgccagtaat cggttaattt atttcgggtt tcttgtcctg tcattcgttg ctgtatccac
    25501 ttttcgctac gacctaattg atgccagttt tctcttgcac ggtcgaggct ttgttccggg
    25561 tccgatattt ctttcattct ttcgtaaccg gtttttgcaa gccactgctt aaatggttct
    25621 gccttcggtg acggtataga ttgaataatg cgcagtaatt gttcggtatc ggcaacatct
    25681 gttgcatact ttttaccatc atcagatttt aatttcagtt gtccgatttt ttcggacaac
    25741 tcacttcctt cctttatcag cttggctttt aaatcgctcc agtattttct tgggcggtcg
    25801 gttccagtga gtatttcaat tacatcgata attgaaaaat accatttctc ctctttttca
    25861 tcccaatgtg ttcttacttt tttttgttca aataatttta tgctattgct cattgtgtca
    25921 atttattttc gtttttataa ttgctgacag atcactttct tttctccaaa tataactact
    25981 tgttttttca tgatgataaa tgttggcaac ttttgcaaat taaaatgtac acaaatttac
    26041 accaccacct aatgtcattc aggaggaacc ttatttctaa aaacgaaatt tttgttcaat
    26101 agcaaaaatt atttcttcct ccacttcccc caaacaattt caaaatccgc ttccgccttt
    26161 ttcgcatcag caatcatttg agtagttggc ggcatatcgc tttcttgcaa aagggaaaga
    26221 attgtaataa atgtgttttg atatttcggg aattcttttt cgaaagttgc cagcgatgtg
    26281 tcttttttaa tttcgatttc tttcaaagca tcaatacact tttttgcatt gttataacac
    26341 atcacactta catcgtgttg cgtttgtaaa tcgttcacat ttgtttttac acgtggatcc
    26401 attttaatag tgagtggttg cgaagtttgc ttaccatcaa cggttaaccg aacagtgtaa
    26461 tttccgggca ttgcaaatgg tgaagtaaca tccggtgcag tattcatgta tatggccgat
    26521 ataggataag ttgccggcac atcgagtggc ttgtaatgca tatcccacat aaagcggtgc
    26581 gcacctgcac cggatggcaa tatttgttga ggacgtatcc agtaatgagg gatatttaca
    26641 tcaggtattt tatacatagt atctatgctt gtatacgttc gtattacttc tgctttagca
    26701 tttacaattt ctaatttcac caatgatgat tttgccggaa gataataatc aatcatggca
    26761 ccatcaggtg gattttgccc tgccggttct tcttgtggta gtggagtatc gagatacatg
    26821 caccaacgca cacgataggt atcttgcggt ttgtacaaag tatattttgc ctttattaat
    26881 tctgttgtaa cctgacgcaa tggagtaata ttatccagaa tccaaaaact gcggccatgt
    26941 gttgctacca ccaaatcatc atcttttatt actaaatcgc gtatagatgt tgcgggcata
    27001 ttcaatcgca acgattgcca gttttcgcca tcgtcaaacg aaaccgatac agcattttcg
    27061 ctgccggcaa aaagtaatcc tttgcgtttg tgatcttctt tcaccacatt gattggaaaa
    27121 ttcgataatc catttacaat ttctttccac gtttttcctc catcaattgt tttgtaaatg
    27181 tgtggtcgct gatcatcgag ccgtatacaa tttactgcgg catatgctgt gtttgcatcg
    27241 gtgtggcttg catccatcaa cgaaatttta ctccagcttt ttattgccgg tggtgtaaca
    27301 tttgtccacg tttttccacc atcgcgcgtt acctgaatta agccatcatc ggtaccgcac
    27361 caaatagtat tgatgtcaac atacgatgga gctactgtat aaataacacc acgccttggc
    27421 atcgttttca tttcttcttt tgtataaata ccaatgcaag atggaatatc atattgctcg
    27481 cgcgttaaat caggactgat aatatcccaa ctattaccac catttaaagt tttaaataaa
    27541 acattgccgg catagtaaag tgttttgttg tcgatgggat taaacaacac tggtgccgta
    27601 cgtataaaac gatacttacc actgcgcact gcttcgggcg aaatgttttg cgactgtcct
    27661 gttcgtttat cgaagcgcgt aatttttcct ccatacacaa tatttggatc caacggatcg
    27721 gcaacaacat aaccatactc ttcggcacca actggctgcc actcacgata cgaaatttgt
    27781 ccatcattac cacgcgatgc aatacctacc gaaccacttt cttgttgacc actatatacg
    27841 ttgtatggaa aatcgttatc gcaacttaca tgataaaatt gtgctgtggg ttgattgtac
    27901 caactcgaaa aagtttcgcc accattcacc gttactattg caccctgatc acttgcaagg
    27961 agcatgatgt tattgtcggt aggatttatc caaatacggt gataatcatc gccaccgggc
    28021 gcaccacgaa aggcactcca tgttttacca ccatctatac ttttccaaac aaccacatcg
    28081 gcactgtaaa ctatatcagc attttttgga tctactttaa cttcggcaaa atcggaacca
    28141 cgttcccata gtcttccatc gttgttcatc aatgcccatg tttcgcctgc atcatcactg
    28201 cgatatatgc cacccatttt tccggcatca acagttgcat acatgcgatt agaatttgtt
    28261 ggtgcaatgc aaaatcctat acgtcccaat tcctgatcgg tagttggtaa accggcagtt
    28321 aattttttcc atgtagtacc gccatctatc gatttatata atccactttc ttttccgctc
    28381 cacatgccgt tttcccaagg cgctaatctg ttggcccaca tatcggcata cactatgttc
    28441 gaattgttgg gatcaattgt tacttgcact gcaccggtgt tttcatcctt gtataaaaca
    28501 cgttcccatt tagcaccacc atctgttgtg cgataaacac cacgctctgt atttgctcca
    28561 taagggtgac caagtactgc cgcaaaaaca cgattctcgt ttttgggatc gatggcaagc
    28621 cctccaattt gttgaccgtc tttgagccct gtattcttcc aggtcttgcc accatctgtc
    28681 gatttgtaaa tgccatcgcc taccgataaa tcaggacgtt gcaacccttc accacaaccc
    28741 acataaatta catttgggtt ggatggtgat acaactacat ctccaaccga gcctgtaggt
    28801 tgctcatcga atataggttt ccatgtgcgg ccaaaatcat ttgttttcca cacgccacca
    28861 ttatttactc caatataaaa atcattgggt tgttgcggta cgcccactgc accaacggtt
    28921 ctgccaccac ggtgcgggcc tatcatgcgc cattgcattg catcgtaata ttttttgtcg
    28981 atgggctgag cggtaatttc attcgcaaaa tttaggagtg caaatgcaaa tgcaataatt
    29041 tt
//
LOCUS       DAORQA010000016        28445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_394_length_28445_cov_29.252413, whole genome shotgun sequence.
ACCESSION   DAORQA010000016 DAORQA010000000
VERSION     DAORQA010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 28445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28445
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_394_length_28445_cov_29.252413"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="PK736_02610"
     CDS             <1..1597
                     /locus_tag="PK736_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018618273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRC32315.1"
                     /translation="LEHNHDEKLLHNLHDWQHEFEVLGGYDIMHKSASVLEALGFSTE
                     DLKRPLRTFSGGWRMRVMLAKILLCKPDLLLLDEPTNHLDLPSIQWLEQYLTDYDGTF
                     VVVSHDRYFLDKVVTKIAEVANQKITLYSGNYSFYLEEKAGREALQQNRFTNQQKYIK
                     EQERLIERFKAKASKATMAQSRMKMLARLETVDEVESDDAAIRIKFSFDVQPGKIINR
                     LNIKSKRYDSMEIIRNSEIEIEKGDKIALIGANGKGKSTMLRIINNSEPFEGTHEWGY
                     NVVPAFFAQHQLEALNLKNTLMEELQHHAPMETDNTLRGLLGAFLFNGDDVFKKVKVL
                     SGGEKSRVALAKTILTKANFLLLDEPTNHLDMKSVNTLIKVLEDYEGSFVVVSHDRYF
                     LSQIANKIWYIEDKVLKQYPGTYDEFEYYKSQQKQQAAAETVKPLKAAKVVETNNDGD
                     KEKKKQQTKLNNRIKSLDELSAKAKNELQQIIADMSNGDVVSNGEKVKAMAQMMKMKE
                     NEIAKIEAEWEDVYTQLMAIDDN"
     gene            1621..2931
                     /locus_tag="PK736_02615"
     CDS             1621..2931
                     /locus_tag="PK736_02615"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HRC32316.1"
                     /translation="MTNNLKFILLFLLCYSNIVAQGNESDAAYNNNHIRYQNYIYKPN
                     IKTVICEPTAAKLMLPMFDLYGDATLTLKFDDLDADFKNYNYTIQHCTPDWQPSDIVE
                     SDFVTGFNNERISNYKTSFNTLVKYTHYELTFPNENLKPMISGNYILTVFQDDDKENR
                     VLTQLFMVFENVVTIAQNIHRATNPELRSTSQEIDFVINLNKLFLPNPFTDLKTVILQ
                     NGRWDNAIKNLRPQFIRTDELEYNYDKENVFEGGNQFRRFDTRSIRYQTEFIKDIAYN
                     DSLKMYDVVLLPSQRRSAERYVSEEDINGKYLIKYQEGNNDDLEADYVNVTFSLKNLY
                     GSDSANVYVFGALTNWELSPANRMKYVSELGLYYYTTLLKQGYYNYALVTDDINNSTA
                     SFTEIEGNHWETENEYTILVYHQPTNKRYSSLIGVQKISSSRIY"
     gene            3016..3402
                     /gene="apaG"
                     /locus_tag="PK736_02620"
     CDS             3016..3402
                     /gene="apaG"
                     /locus_tag="PK736_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HRC32317.1"
                     /translation="MTIAITKGISVSVETIFQREHSNEKMHQYVFAYRILIENNSNVT
                     VCLRRRHWHIIDGDGTKREVEGEGVVGEQPILEPGSKYKYVSGCNISCEMGKMHGTYT
                     MENMEDHTFFDIDIPAFMMEVPFILN"
     gene            4177..6240
                     /locus_tag="PK736_02625"
     CDS             4177..6240
                     /locus_tag="PK736_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32318.1"
                     /translation="MKNIILLILLITFYTTNFHAQNPLVKQWDKRFGGTDFDLLSSLQ
                     QTSDGGYILGGISLSGIGGDKTQPSWGSYDYWIVKIDSLGNKQWDKRYGGTDSDLLTS
                     FQQTSDGGYILGGRSESGISGDKTQPSWGNDDYWIVKIDSLGTKQWDKRFGGTSYELF
                     YSLQQTTDGGYILGGLSWSGISGDKTQPSWGSYDFWIVKISSLGNKQWDKRFGGASDD
                     ILYSLQQTTDGGYILGGYSWSGISGDKTQPSWGVRDYWILKIDSLGNKQWDKRFGGTS
                     IDWLNSLQQTTDGGYILGGMSNSGISGDKTQPAWGNSVDYWIVKIDSLGIKQWDKRFG
                     GTSDDEGIGYISQTIDGGFLLSGDSYSPISGDKTENNLGPEQTWVIKTDALGNKQWDK
                     TVFTNGHDEGGLAIKTKDGCFALANYTYAGIGGDKTQASQGSWDYWIIKFCDTTLAVI
                     PSVNLSASDTIFCEKQSINFYDLSLNNPTSWQWYFPGAQPDTSTLQNPTGIYYPSYGQ
                     FDVSLKACNAGGCDSLYFPNFITELQNPPAPTVTVNGSLMCSSPAFAYAWYETANLTL
                     LLSSNQCYQPTLPGNYFVIIADSNGCSKPSATIIITAMQNENNHACILTYNGSQLLVQ
                     GNCITDSESYVYIYDAQGKEIYVSQLSSSSVNLPLTNNGIYFYLVVSHGVTSQGKFSF
                     QNRMD"
     gene            7441..7890
                     /locus_tag="PK736_02630"
     CDS             7441..7890
                     /locus_tag="PK736_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRC32319.1"
                     /translation="MTLVKFKNLSNENGLKVNPAFGNLYGDMLSNLFPANFGSITTPA
                     VNIKETNKNYVLELAAPGLKKEDFKISLDKDVLTISFENKNETNETNEKYTRKEFVYN
                     SFERAFTLPEHVEQAKINATYNNGILVLDLPKAEAAQPKPAKEIKIS"
     gene            complement(7936..8601)
                     /locus_tag="PK736_02635"
     CDS             complement(7936..8601)
                     /locus_tag="PK736_02635"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRC32320.1"
                     /translation="MLKKFLKPFLNYVYKPFVKLYLRKKRTVQISGLTIEVWPGVFHP
                     TLFYSTKYLLQFLSDFDLNNKRLLELGCGTGTISCFAATKNAIVTAIDINQTAVANCT
                     KNAMLNKVKVLCLQSDLFDKITNATFDYIIINPPYYAGNATSDEEQAWYCGSEYQYFE
                     KLFFQLKNRNDDATVFMILSDECAIEKIDAVGTNYSLELKVVKEYSNILERNSIYEIK
                     KRG"
     gene            complement(8614..10920)
                     /locus_tag="PK736_02640"
     CDS             complement(8614..10920)
                     /locus_tag="PK736_02640"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC32321.1"
                     /translation="MKNILLFIFFLFTISINISQAQNAIVRGFVYEKESGEAVLFVNV
                     YLKGTKYVAQTDVNGYFSINKVPPGDYFLTVTAMGWDTLSVPVKLVMGETLNKKLFLV
                     KRSVNLRTVEVSAEKTEQKSETKVGVQKITPRDIKRLPSIGGEPDLAQYLQVIPGVIF
                     TGDQGGQLYIRGGTPVQNKVLLDGMIIYNPFHSIGLFSVFDTDILSNTDVYTGAFPSK
                     YGGRISSVMDITTRDGNKKHLSGKISTNTFQSKLLVEGPLKRAKTEEDGSASFIFSIK
                     DSYLDKTSKLFYNYVDKENGLPFKFRDIFAKVSTSNVSGSKISLFGFNFNDEVNYKQV
                     SKFSWGSNGFGSKFVVVPGGSQTLIDGNFAYSKYSMQLKEADEKPRLSETNGFNMAFN
                     FTYFSSNDEFKYGLEVLGFKTNFKFYNLLGNKFEQAENTTEFGAYLNYKKVWSKVVLN
                     PGFRMHYYASLGEAVPEPRISVKYNMFDKFRLKAAAGTFSQNLLSATSDKDIVNFFYG
                     FLSAPESLPEEFNGEEVKSNLQKARHVIAGFEWDLPLHLSLNVEGYVKRFNQLININR
                     DKIYEDTQENADKPDELKRDYRIETGTSKGLDFVLKYDYKRFYIWAVYSLGFVDRFDG
                     KITYQPNFDRRHNINVVTSLTFGKSLLWEADMRWNFGSGFPFTPTQGFYPYLNFGDGI
                     FTDYTTVNGYLGVLYGDFNSRRLPYYHRLDFSIKRTFVVTKKSNIEASFSISNVYDRR
                     NIFYFDRIRYKRVDQLPFLPAMGVNWTF"
     gene            11187..13082
                     /locus_tag="PK736_02645"
     CDS             11187..13082
                     /locus_tag="PK736_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HRC32322.1"
                     /translation="MAYKSTRDFIAALESAGELVRVKEFVDPKLEITEVIDRLSKHDG
                     PAVLFENTGTDFPILINAMGSEKRMSMALGVAHLDDIAKQLEQLFKTITAPKNSFLEK
                     LKVLPQLGAISSWMPKQISGRGACQEVVMPQPDITKLPVMTCWPEDGGPFLTLPIIQT
                     LDPVTNIRNVGLYRMQVFEPTITGMHWHRHKVSARHFNEYKKLNRRMPVSVTLGGDPC
                     YAYCATAPLPDGVDEFILAGFIRKKRVELVKCLTNDLYVPADSDFVIEGYVDPNEDFI
                     LEGPFGDHTGYYSLADFYPKFHITCITHRKDAVYPSTIVGIPPQEDAWIGKATERIFL
                     APIKMTMLPEIIDMVLPVEGVFHNLVIVKIHKEYPGHALKVMNSLWGAGQMMFTKMMI
                     VTDGDCNIHDVMSIAQHISKNVNAESDFYFTQGPTDVLDHACTRMSFGGKVGIDATIK
                     WDEEKPLHTNTNKQSTPNQFVEIKNLFPEITNVNANLLASDISMVFISVEKNRKGHIA
                     ELSKQLFELDAFANVNVLILLEANIDVSDIGNAVWRFANNVDPRRDHIMVKHHQTTSV
                     AFDGTRKTLMYDDFERDWPNILVSDDDTIAKVDAMWSKLNLGGLIASPSLRYRNQVYK
                     GGAVVKD"
     gene            13436..14458
                     /locus_tag="PK736_02650"
     CDS             13436..14458
                     /locus_tag="PK736_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRC32323.1"
                     /translation="MGFFDLLTQEIAIDLGTANTLIIHNDKVVVDEPSIVAIDRTTGK
                     VLAVGKQAMMMHGKTHENIKTVRPLKDGVIADFDAAEHMIRGMIKMINPGKRLFQPSL
                     KMVICIPSGITEVEKRAVRDSAEHAGAKEVYLIHEPMAAAIGIGIDVEEPNGNMIIDI
                     GGGTSEIAVIALGGIVCDQSIRVAGDGFTSDIWDYMRRQHNILIGERSAERIKIEVGS
                     ALPELDNPPADFAIHGRDLMNGIPKEITVSYNEVAHALDKSISKIEEAILKALEMTPP
                     ELSADIYRTGIYLTGGGALLRGLDKRISMKTKLPVHIAEDPLRAVVRGTGIALKNIGR
                     YKFLIP"
     gene            15071..15907
                     /gene="mreC"
                     /locus_tag="PK736_02655"
     CDS             15071..15907
                     /gene="mreC"
                     /locus_tag="PK736_02655"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRC32324.1"
                     /translation="MRNLLQLLWKNNFLILFVLLEILCLYMIVQNQYYQRAVFINSSN
                     NVVGNVLSKINSVKQYLFLTQKNTELQHQNAKLLASDSTSKFSLTTVKDTSHNKDLIQ
                     QYTYTVAKVINNSVHHSNNYLTLDKGSLNGIERDMGVVGPLGVVGVVKEVSPHFCTVL
                     SLLHKEWKINGKLKKSGEFGPVAWNGKPLYANLEEIPTSARITKGDTIVTTSFSTIFP
                     EGIHLGYITSSKIEPAKSTLSIEMKLATNFYSVGEVYIITNRLKTEQKELEHKTDSTH
                     VD"
     gene            15897..16415
                     /locus_tag="PK736_02660"
     CDS             15897..16415
                     /locus_tag="PK736_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRC32325.1"
                     /translation="MLIEILLIIVRFILLVTLQVVVLDNVQLSYFINPYLYVLFILLL
                     PINMPRGLVLIIALILGFVVGAPTNTSGIHGAACVFIGYMRPFVLKLIAPREGYESDA
                     KPTMREMGLNWFFSYAAIMVFLHHTVLFFLEVFRLSEFFTTIMRIVVSSLFTLAFIMI
                     SVYLFYKPKGQR"
     gene            16412..18253
                     /gene="mrdA"
                     /locus_tag="PK736_02665"
     CDS             16412..18253
                     /gene="mrdA"
                     /locus_tag="PK736_02665"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRC32326.1"
                     /translation="MIRETGQQNVILGIFITIILVYIMRLFYVQVVDDEYTASANNNV
                     LRYVTVYPPRGVIYDRKGHVLVHNEVFYDLMVIPRQAKNIDSTEFCSLLGISIEDFSA
                     RMAAAKKYSSIKPTVFEKQISKINFSTLQEKLYKFPGFYAEPRTLRYYPKPAAAHLLG
                     YVGEVDERLIAKNAYYKMGDYIGISGIEQSYEKELRGQKGLRIMMVDVHNREKGSFRD
                     GALDTASVEGQNLVLSLDGDMQLYAERLMQNKIGGIVAIEPATGEILAVVSSPSYDPN
                     LLVGRVRAKNYVQLLQNNLKPLFNRAMQAYYPPGSTFKLINALIAFNEGFINGETRQT
                     CSFRVGSHSIHCHPHPGPADLRTAVQYSCNPYFCKTFVNYVENDKWVNTEDGYEHWRK
                     HVLSFGVGRKLGIDIPHELPGLVPTVKYYDKYFGKNHWSATTVFSLGIGQGELGITPM
                     QMANIVCIIANKGYYKIPHVVKYIGNEKNVPIEFTKKNFCDVQGEAYDVVIDGMQRVV
                     EAGTARVARLDSIIVCGKTGTAQNPHGKEHALFVCFAPRVNPKIAIAVMVENAGYGGT
                     WAAPIASLMMEKYLTDTIKRPDLEKKILNAVILPTPKVIPDSLQIPD"
     gene            18253..19515
                     /gene="rodA"
                     /locus_tag="PK736_02670"
     CDS             18253..19515
                     /gene="rodA"
                     /locus_tag="PK736_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRC32327.1"
                     /translation="MRQQKSIFDNIDWTTVLIYLALVIFGWINIYAAVYDERFQSIFD
                     TTQRYGKQMLWIVLAIIISLAILVVDGKFYEAFAYYIYGFFMLALLLVLLIARDVKGA
                     VSWIDIGSFKLQPSEFTKFGTCLALAKFLSEPYTRMTENKTKIIAALICLVPMALILM
                     QNDTGSAMVYASFIFVMYRAGLSQNFLIIGFITILIFVLALMIPKLVLVGVIIGVSAL
                     GALFLRRTMKNILMLAFIAIVGCAMVYGVDYVFNEVLQTHQRERIEVLLGKRADPKGA
                     GYNVNQSLIAIGSGGLTGKGFLQGTQTKYDFVPEQATDFIFCTVGEEWGFIGTTILMA
                     MFLWLLIRIVMIAERQKNNFVRMYGYGVASIIFFHIFINIGMTIGLLPVIGIPLPFFS
                     YGGSSLWSFTILLFILIKLDSYRMFVLR"
     gene            19807..21270
                     /locus_tag="PK736_02675"
     CDS             19807..21270
                     /locus_tag="PK736_02675"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRC32328.1"
                     /translation="MLFNSLSFAVFLPVVFVLYWFVAKNKLVWQNLLLLFSSYFFYAC
                     WDYRFLFLLIFSTLLDYLTGLKMHQTTNTKMRKFWFWLSVIINLGFLAVFKYYNFFAD
                     TFVTAISKLGLHAELSTIQVILPVGISFYTFHGLSYVIDIYRKKIEPTRNFIHYTVFV
                     SFFPLLVAGPIERATHLLPQIKVLRTFNYHYAVDGLRQILWGLFKKIVIADNCAQFAN
                     IIFNDSDSYHGTTLILGALCFTFQIYCDFSGYSDIALGTARLFGIELLRNFAFPYFSR
                     DIAEFWRRWHISLSSWFRDYLYIPLGGSSGGMWMRIRNTFIIFIVSGFWHGANWTFIF
                     WGFLNACYFLPLLLADKNRTNLEIVAMDKWLPSWKEFLQISITFSLTVFAWIFFRAQN
                     MHHAFTFIQGIVEGLAHKSAYIETLNFLRWNIGKSIPILVIIFMLVEWIGRSQQFAIQ
                     NLGLQWPRTVRWGLYYIIIIAIFIFGGSEQQFIYFQF"
     gene            21270..22283
                     /locus_tag="PK736_02680"
     CDS             21270..22283
                     /locus_tag="PK736_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32329.1"
                     /translation="MMSKFIRTLLSFIAIVAIPFCILVGLYFYFDPFMVVRNYKNFSH
                     SHVALNRDYVSSQIFLQNYPKHKFDSYIFGSSRTMAFEPNVWKTKLEADARPFMFDAA
                     AESIYGIHSKIKYLDDHNIPIRYALIIVCHDRTFEHSTDEAGFLFIKHYKFSKNGFIN
                     FHKTFFLAYLDPHFLKAYFAYQNKKKYEKWMAKYMVEWDIEYDTVNNQMFYYKDIEKQ
                     LLTDPDAYYNTRMKNFFAPQKKETIDSKNLITASQIKMMKEIIAIFEKNKTKYKVVVS
                     PLYTKVKFSHGDTKVLQSIFGKQFYDFSGDNIYTSTKYNYYEMSHYRKTAGRQIMDSI
                     YHP"
     gene            complement(22313..23692)
                     /locus_tag="PK736_02685"
     CDS             complement(22313..23692)
                     /locus_tag="PK736_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32330.1"
                     /translation="MQYLSILDLFTTPVFFILIYFISRFYAKGKVEKYPYYKYYVPGV
                     MMKCIGGVSLCLVYCLYYGGGDTTGYFFNALCMKYLLLKNPSYFYDVMFHYAGKDQWF
                     YFDNDTNYPYYLGDPPAFFVVRLISPVIFFCFDSFVLSSLIIGWFSFNGVWKLFEVMI
                     DQFPKLEKQFAMAIFFVPSVIFWGSGILKDTITLSATGYFVFSIYQIFFKRQKIFANV
                     LLLFVSVYLILSIKPYIFVGILPGTLIWLLGVTLGKIRGTMMRAFFLPILLIVMIGGG
                     FGVLLFMSDSLSAYSLDSILEKAVDTQQDLKRDYYQGNTFDIGDYDATLSSMLAKAPL
                     AINASLFRPFILEANNPVMLASSIENLWILFFTLQVLWRTKVINFFRYIFSEPMLIFC
                     FTFSMFFSFSVGITTSNFGSLVRYKIPMIPFYMCLLYMINEMARLKKARDMQEDVVHD
                     EGYIPSLKN"
     gene            complement(23696..24595)
                     /locus_tag="PK736_02690"
     CDS             complement(23696..24595)
                     /locus_tag="PK736_02690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRC32331.1"
                     /translation="MNNNDLGDTLQTSAKTSFLNFFRNIFKIKFLENIIVTHTQQKPT
                     DAFVAKIIPNNYQYGKPTWRTATRNGINYKLDISDYMEYCIYYGLQNEPRHILYDLAK
                     QGQTIFDIGTNIGETLMNFARINSQGKNYGFEPVPHLYQRALQNISNNSFTNISLNNV
                     ALSDREEVLHFHQPTLKNSGSVYMIKGDISSPDQKVQALSLDTFVNNNSIAKIDLIKI
                     DTEGFEYNILSGGKESINKFHPVLFVEINDSNLHRQGNSAKALFELLISWNYKIYDAI
                     TNEQLTASYNFENKHFDIIARYN"
     gene            24666..25805
                     /gene="rffA"
                     /locus_tag="PK736_02695"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             24666..25805
                     /gene="rffA"
                     /locus_tag="PK736_02695"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HRC32332.1"
                     /translation="MTDFIPFNKPYKAGREFEYISEAINRGHLSGDGYFTRKCQSWFE
                     QTYKFNKTLLTTSCTDALEMCALLANTNPGDEIIAPSYTFVSTVNAFVLRGAKIIFAD
                     SSPLHPNIDETQIEKLITPKTKAIVVVHYAGVACNMNPILDLARKYNLYVIEDAAQAI
                     DSFYYDKPLGGIGDMAAFSFHETKNIICGEGGMIVINNKQFEKRAEIIREKGTNRSSF
                     FRGEVDKYGWVDVGSSFLPSELLAAYLYAQIEAITDIQKKRMHIWKKYFDGLASLTQS
                     EKIKFPFMPTYATNNAHMFYIECRNLDERTALVKYLKSQNILAVFHYLSLHKSPYYAS
                     QYNGDDLPNADRFTDCILRLPLYYELTDSQLEYIIHHIHVFFNDN"
     gene            25823..26512
                     /locus_tag="PK736_02700"
     CDS             25823..26512
                     /locus_tag="PK736_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HRC32333.1"
                     /translation="MNGIITQSNYIPWKGYFDNIAQCNVMVVYDDMQYTKRDWRNRNY
                     IKTPQGLKWLTIPVEVSGKYLQKINETKISNNEWNKEHWAMLKQYYRDAPYFSEFATW
                     VEGLYNDAIFNTLTEINVHFLKAVCKFLEIETQFIDSRQFTLVEGKTERLVDICKKCG
                     ITNYYSGPAAKNYMDENLFLNENITVHYADYSGYKEYPQLFSPFEHGVCIWDLIFNVG
                     NNSKKYLKNIV"
     gene            26509..27441
                     /locus_tag="PK736_02705"
     CDS             26509..27441
                     /locus_tag="PK736_02705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC32334.1"
                     /translation="MSQPVELSIVTTVYKSEKYLPEFIALILQSIKEIGINNFELLFV
                     LDGITDASKEYLLARKKDIPQIVVVELSRNFGHHYAASAGLNTASGDLVFLIDCDLEV
                     SPTVLTAFFHELKKQSCDVVYGVQKMRKGGFTERFFGGLFWKMINALSDTKIPHNIVT
                     ERLMTRNYIDALNSMGDKNLFMAGLMYWAGFNQVGVPVDKKQRTGQSSYSFGKRISLV
                     FEAVTSFSERPLKILFKIGLYITALSVIASVYLLIKKILYPNTIFMGYTSVFTLILFS
                     LGMLISSIGIVGIYLSRIFKQVQNRPLFIIKKIY"
     gene            27449..28189
                     /locus_tag="PK736_02710"
     CDS             27449..28189
                     /locus_tag="PK736_02710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC32335.1"
                     /translation="MENLDSYKEAYNQSFNFYDENQWYLAQYAKYLCAKITDTKASSI
                     LSLGIGHSVVSNSIIQLLNTGNIQYDIVEGSPDIINKFNATYHNISMKVYESYFETFT
                     PDKKYDAIEMGFVLEHVDDPLLVLKKFKNFLNDNGTMFIAVPNAKSLHRLIGHHAGLL
                     PDMYKLSKYDLELGHKRYFDLDSITNLATEAGLRVTMQRGLMLKPITGEQMKKLDWQK
                     NIIDALFIVGEHYPEISNCIYLEAVINK"
     gene            28186..>28445
                     /locus_tag="PK736_02715"
     CDS             28186..>28445
                     /locus_tag="PK736_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32336.1"
                     /translation="MNVLVTCGGGLQGLTLYKELNALQSKFNITSHLFDIHAENISKY
                     FFDYFFQCPPVADSEKYISALFDYCTNHKIDFIFPATTYDLL"
BASE COUNT         9228 a         5184 c         5451 g         8582 t
ORIGIN      
        1 actggaacac aatcacgatg aaaaattatt gcacaaccta cacgactggc agcatgagtt
       61 tgaagtgctt ggtggttacg atatcatgca caaatcagct tcggtattag aagcattagg
      121 atttagcacc gaagatttga aacgcccttt gcgtactttt tcgggaggat ggcgtatgcg
      181 tgtgatgctg gcaaaaatac ttttatgcaa gcccgatttg ttgttactcg atgagcctac
      241 caatcacctc gacttacctt ctatacaatg gcttgaacaa tacctcaccg actacgatgg
      301 cacttttgta gtagtatcgc acgacaggta ttttcttgat aaagtggtga caaaaattgc
      361 tgaagtggcc aaccaaaaaa tcacgttata tagcggcaat tattcttttt atcttgaaga
      421 aaaagctggg cgtgaagcat tgcaacaaaa ccgctttacc aatcaacaga aatatataaa
      481 agaacaagag cgacttatag aacgatttaa agccaaggca tcgaaagcca ccatggcaca
      541 atcgcgtatg aagatgttgg caaggctcga aacggtagat gaagtggaat cggatgatgc
      601 cgccatacgt ataaaatttt cgttcgatgt gcaacccggt aaaattatca atcgcttaaa
      661 tataaaaagt aagcggtatg attccatgga aattattcgc aacagcgaaa tagaaattga
      721 aaagggcgat aagattgcac tcataggtgc caatggaaaa gggaaatcga ccatgttgcg
      781 catcatcaac aatagcgagc ctttcgaagg cacacatgaa tggggataca atgtagtgcc
      841 ggcatttttt gctcagcatc aattggaggc gttgaacctg aaaaacacat tgatggaaga
      901 actgcaacac catgccccca tggaaaccga taatactttg cgtggtttgc ttggagcatt
      961 tttatttaac ggtgacgatg ttttcaaaaa agtaaaagtg ctatcaggtg gcgaaaaatc
     1021 gcgtgtggca cttgcaaaaa ccattctcac aaaagccaat ttccttttgc tcgatgagcc
     1081 taccaatcac ctcgatatga aaagtgtgaa cacactgata aaagtattgg aagactacga
     1141 gggttcgttt gtggtggtat cgcacgacag gtatttctta tcgcaaattg ccaataagat
     1201 ttggtatatt gaagataaag tactgaagca atatcccgga acttacgatg aatttgaata
     1261 ttacaaatcg caacagaaac aacaagccgc agccgaaacc gttaaacctt tgaaggctgc
     1321 aaaagttgta gaaacaaaca acgatggcga taaagaaaaa aagaaacaac aaacaaaact
     1381 caacaaccgc ataaaatcgc ttgacgaact ttcggcaaaa gccaaaaacg aattgcagca
     1441 aataatagcc gacatgagta atggcgatgt agttagcaat ggcgaaaaag taaaagctat
     1501 ggcacagatg atgaaaatga aagaaaacga aattgccaaa atagaagccg aatgggaaga
     1561 tgtttataca caattaatgg caatagacga taattgattt cggaatttaa ttttaaaata
     1621 ttgacaaaca accttaagtt tatattgctt tttttgcttt gctattcaaa tatagtagcg
     1681 caaggaaatg agagcgatgc tgcatacaac aataaccata tacgttacca gaattatatt
     1741 tacaaaccta acattaaaac agttatatgc gaacctactg ccgcaaagct gatgttaccc
     1801 atgtttgatt tgtatggcga tgcaacttta acattaaagt ttgacgacct cgatgctgat
     1861 tttaaaaact ataattacac catacaacat tgcacgcccg attggcaacc gagcgatatt
     1921 gtagaatccg attttgtaac cggtttcaat aacgaacgaa tttctaatta caaaacatcg
     1981 ttcaatacgc ttgtaaaata cacacactac gagctcactt ttccaaacga aaacctgaaa
     2041 ccaatgatta gcggaaatta tattttgact gtgtttcagg atgatgataa agaaaaccgc
     2101 gtgcttactc agctatttat ggtttttgaa aatgtagtta ccatagcaca aaatattcac
     2161 cgtgcaacca atcctgaact ccgaagcacc agtcaggaaa ttgattttgt cattaacctt
     2221 aataaattgt ttctgcccaa tccgtttact gatttaaaaa cggtgatatt acaaaatggc
     2281 cgttgggata atgcgataaa aaatctgcga cctcagttta tacgcaccga tgaattggaa
     2341 tataattacg ataaggaaaa tgtgtttgaa ggaggaaatc aatttcgcag attcgatacg
     2401 cgtagtattc gctatcaaac agaattcata aaagacattg catacaacga ttcgttaaaa
     2461 atgtacgatg tagttttgct gccttcgcaa agacgctctg ccgaaagata tgtaagcgaa
     2521 gaagatatta acggaaaata tttaataaaa tatcaggaag gaaataatga tgacctcgag
     2581 gccgattatg taaacgttac cttctcttta aaaaatttgt atggctccga ctcggcaaat
     2641 gtgtatgtgt ttggtgcatt aacaaattgg gaattgtcgc ctgccaaccg tatgaaatat
     2701 gtttctgaat tgggtttgta ttattacaca acactattaa aacaaggcta ttacaactat
     2761 gcacttgtta cagatgacat taataattcc accgcttcgt ttacagaaat agaaggcaat
     2821 cattgggaaa cagaaaatga atatactatt ttagtgtatc accaacccac caataaaaga
     2881 tattcttcat tgataggagt acaaaaaatt tcttcttcgc gcatttacta atattgtttg
     2941 tgtattattt tttcgaaaat ttatttgcgc atgtaaaaac aacctacatt tgtgcacaac
     3001 taaagaatac gaacattgac aattgccata acaaaaggaa taagtgtaag tgttgaaact
     3061 atttttcaac gtgagcattc taatgaaaaa atgcatcaat acgtttttgc gtatcgcatc
     3121 cttattgaaa acaatagcaa cgttacagtt tgtttacgca gaaggcattg gcacattata
     3181 gatggcgatg gtacaaagcg tgaggtagaa ggcgaaggcg ttgtgggcga acaacccata
     3241 ctcgaaccag gcagcaaata caaatacgtt tcaggttgca atatcagttg cgaaatggga
     3301 aaaatgcacg gcacctacac catggaaaat atggaagacc acactttttt cgatatcgat
     3361 attcctgcct ttatgatgga ggtacctttt attttgaatt agcgaataag ttctgagttc
     3421 tgagtttgtc gtttagcgtt ttttgttcct tgttgtaagt ttagcgtttt tagtttttag
     3481 ttccgtgttc tgcattttaa gtttccacaa ttatttctgt tcaatcattt attagtgcca
     3541 cacctaaggc atgttttatt tttttgcaaa atattttttg atgttttata ggatggatta
     3601 aaatccatcc ttacaatatt gagtcgagcc tacggctcta ttgctttcgc gtatgttgct
     3661 gcaccacaat atccccgaag gggataaata ttaatagcca tgggtgcaac ccatggtggg
     3721 gtaaaaccca tggtatcaag aaattgaatt gagggtacaa tcaaggtttg tggggacaca
     3781 aaccttggcg atggatgaca caaaccttgg cgatggagga cacaaacctt ggcggtggag
     3841 gacacgaaca ttggcggaaa atggtttgtg caacaaaggt tgcacaacaa gaatcccgta
     3901 gggatgaagc aagttgtagc catgggtttt aacccatggc atttcgcaat cataaataaa
     3961 acccatggta ttaagaaatt gaattgaatt agcaatcaag gtttgtgggg acacaaacct
     4021 tggcgatgga ggacacaaac attggcgatg gaagacacaa accttggcct aaaaaaagca
     4081 aatcaacagc cgcaataaaa aaaaacaaca tcaaataaaa aactaatgtt gctctaaaat
     4141 ttttacattt gttacatcaa taaaactcca cctcaaatga aaaacattat cctccttatt
     4201 ttactcatta ctttttacac aacaaacttc cacgcacaaa acccattagt aaaacaatgg
     4261 gataaaaggt ttgggggaac agattttgat ttgctttctt ccttacaaca aacatccgat
     4321 ggtggttata ttttgggagg aatttctttg tctggaatag gtggtgataa aacgcaacct
     4381 tcatggggaa gttatgatta ctggattgta aaaattgact cgcttggaaa taaacaatgg
     4441 gataaacgct atggcggaac agatagtgat ttgctaacct catttcaaca aacaagcgat
     4501 ggaggttata ttttgggtgg acggtctgaa tccggaataa gcggagataa aacacaacct
     4561 tcatggggta atgatgatta ctggattgta aaaattgatt cgcttggtac aaaacaatgg
     4621 gataaacgct ttgggggaac aagttatgag ttattttatt cattacaaca aacaacagat
     4681 ggtggatata ttttgggagg cctttcctgg tccggaataa gtggtgataa aactcaacct
     4741 tcatggggaa gttatgattt ctggattgta aaaataagtt cgcttggaaa taaacaatgg
     4801 gataaacgat ttggtggagc aagtgatgat atactttatt ccttacaaca aacaaccgat
     4861 ggtggatata ttctgggagg atattcctgg tccggaataa gtggtgataa aacacagcct
     4921 tcatggggtg tccgtgatta ttggatttta aaaattgatt cgcttggaaa taagcaatgg
     4981 gacaaacgct ttggtggtac aagtattgat tggcttaatt ccttacaaca aacaaccgat
     5041 ggtggataca ttttgggagg aatgtccaat tccggaataa gtggagataa aacacaacct
     5101 gcatggggaa atagtgttga ttactggatt gtaaaaattg attcgcttgg aattaagcaa
     5161 tgggataaaa ggtttggcgg aacaagtgat gatgaaggca tcggatatat ttcacaaact
     5221 attgatggtg gatttttatt gtcgggtgat tcttattcac caataagtgg agataaaaca
     5281 gaaaacaatc ttggcccaga acaaacatgg gttataaaaa cagatgcgct tggcaataag
     5341 caatgggaca aaactgtttt tacaaatggg catgatgaag gtggtttagc aattaaaaca
     5401 aaggatggat gctttgcttt ggcaaattat acctatgcag gaattggagg tgacaaaaca
     5461 caggcttcac aaggaagttg ggattattgg ataataaaat tttgtgatac aactttagct
     5521 gttattcctt cggtaaattt atctgcaagc gatactatct tttgcgaaaa acaatctatc
     5581 aatttttatg atttgtcgtt aaataatcca acatcatggc aatggtattt tccgggagcg
     5641 cagcccgata cttctacatt gcaaaatcca actggtattt attatccaag ctatgggcaa
     5701 tttgatgtta gcctcaaagc atgcaatgct ggcggttgcg atagcctcta ctttccaaat
     5761 tttattactg agttgcaaaa tccacctgcg ccaactgtta cagtcaatgg tagtttgatg
     5821 tgttcatcgc ctgcatttgc atacgcatgg tacgagacag ccaatctcac attgttatta
     5881 tcaagcaacc aatgttatca gccaacacta ccgggcaatt attttgtaat tattgcagac
     5941 agcaacggtt gcagtaagcc ttcggcaaca ataattatta cagctatgca aaatgaaaat
     6001 aaccatgcct gtatactcac atacaacggt tcgcaattat tagtacaagg caactgtatt
     6061 acagacagcg aatcgtatgt gtatatttac gatgcgcaag gcaaagaaat ttatgtttcg
     6121 caactttcat catcaagtgt caacttacca ctcaccaaca acggaattta tttttacctt
     6181 gtggtatcgc atggagtaac atcacaaggc aagttttctt ttcaaaatag gatggattaa
     6241 aaaccatcct tacaatattg agtcgagcct acggctctat tgctttcgca tcaaaagttg
     6301 cggcaaacaa atcccgtagg gatgttccat tttgtaacca tgggttttaa cccatggtgt
     6361 taaaatccat ccttacaata ttgagtcgag cctacggctc tattgctttc ggataaaata
     6421 atttgatgag cattcaaacc cacaaagttt ttaaaacctt gtgggtttga aatgttctgt
     6481 cctcgccatc ctccaattcc gcgctttctg cttcgattcg tgtccctaca actctggaca
     6541 aatttctgtc ctcggttggc gtccccgcca accgacaatc ccgtgctcgg ttgggttgct
     6601 cgccaaccac tacttatttt tcagttcgca taatcctaaa tggttttaaa aaccagcatg
     6661 gatatagcaa tcaaaaatgt aatttccgct gttgtctacc ttgccaataa ccggttggcg
     6721 gggacgccaa ccgaggacaa aaacctgcaa ggatatagca atcaaaaatg taatttccgc
     6781 ttttgtctac cttgccaata agcggttggc ggggacgcca accgaggaca gtgcagctct
     6841 atggttttcg cgaaagttgc tgcgccacaa aaatccccga aggggataaa tatcaatagc
     6901 catgggcaaa acccatggta ttaagaaatt gaattgaatt agcaatcaag gtttgtgggg
     6961 acacaaacct tggcgatgaa gtgcgcaaac cttggtgatg gaggacacga atattggcgg
     7021 aaaatggttt gtccaacaaa ggttgcacaa caagaatccc gtagggatga agcaagttgt
     7081 agccatgggt tttaacccat ggcatttcgc aatcctaaat aaaacctatg gtattaagaa
     7141 attgaattga gggtgtaatc aaggtttgtg tggacacaaa ccttggcgat aggggacaca
     7201 aaccttggcg ttggaggaca caaaccttgg cataaaaaaa ataaaaaata cgttcggaat
     7261 ttttgtcggc taatatttag ttaatgcgac tgtttggctt ttcattcacc acataaaaag
     7321 aaaaaatgtc atgaaaaccg ctgtggaaca ggctttgatg caaggtgtgc gtaccaatgt
     7381 ttggaagcgc gctaaacatt aggatgaaaa aaaacaaaaa caattaaaaa aaataaagca
     7441 atgacattag taaaatttaa aaacctgagc aacgaaaacg gattaaaagt aaatccggca
     7501 tttggaaatt tgtatggcga tatgttatca aatttgtttc cggcaaattt tggttccatc
     7561 actacaccgg cagtaaatat taaagagacc aataaaaatt atgtgctcga acttgctgct
     7621 cccggtttga aaaaagaaga tttcaaaatt tccttagaca aagatgtgtt gaccataagt
     7681 ttcgaaaaca aaaacgaaac aaatgaaacc aacgaaaagt acacacgcaa agaatttgtt
     7741 tacaattcat ttgagcgggc gtttactttg cccgagcatg tagaacaggc aaaaataaat
     7801 gccacttaca ataacggaat attggtattg gatttgccaa aagcagaagc tgcacagccg
     7861 aagccggcaa aagaaattaa gatttcataa gagagttttt aggggtagtt tgattgaaac
     7921 ggggagtggc gccagtcacc cccgtttttt tatttcatat atgctgttgc gctcaagtat
     7981 gttgctgtat tcttttacaa cttttaattc aaggctatag tttgttccaa ccgcatcaat
     8041 tttttcaata gcacattcat cacttaatat cataaaaact gtggcatcgt catttctatt
     8101 tttaagctga aaaaacaatt tctcgaaata ttggtattcg ctgccacaat accaggcttg
     8161 ttcttcatcg ctggttgcat tgcccgcata gtaaggcgga ttaataatta tataatcgaa
     8221 agtggcatta gtaattttgt cgaataaatc gctttgcagg cacaacactt ttactttatt
     8281 caacattgcg ttctttgtac aattggcaac tgccgtttgg tttatatcta ttgcagttac
     8341 aatagcattt tttgttgcag caaagcatga tatagtcccg gtgccacaac ctaattctaa
     8401 taagcgtttg ttatttaaat cgaaatcgga taaaaattgc aacaaatatt ttgtggaata
     8461 aaacagtgtg ggatgaaaaa cgcccggcca tacttctata gtaagtcctg atatttgtac
     8521 tgttcttttt tttcgaagat atagttttac aaatggctta taaacatagt tgagaaatgg
     8581 cttaagaaat ttcttcaaca tcgtgtatgc ttattaaaaa gtccagttaa cgcccattgc
     8641 aggcaaaaat ggcaattgat ctaccctctt atatcgaata cgatcgaagt aaaaaatatt
     8701 gcgtctgtca tacacattcg atatgctgaa cgatgcttcg atatttgact tttttgtaac
     8761 cacaaaagta cgcttgatag aaaaatcgag gcgatgataa taaggtaatc ttcgcgaatt
     8821 aaaatcaccg tacaacacac ctagataacc gttaacggtg gtgtaatctg taaagatgcc
     8881 atcaccaaaa ttgaggtaag gataaaaacc ctgtgtagga gtgaaaggaa atccggagcc
     8941 aaaattccac cgcatatctg cttcccataa caaacttttt ccaaacgtga gtgaggtaac
     9001 cacattaata ttatgcctgc gatcgaagtt gggttgatag gtaatttttc catcgaaacg
     9061 atcaacaaat cccaatgagt atacggccca aatataaaat cgtttgtaat cgtatttcaa
     9121 cacaaagtcc agccctttgg atgtgccggt ttcaatgcga taatcgcgct ttagttcatc
     9181 gggcttatcg gcattttctt gagtatcttc gtatatttta tcgcgattaa tatttataag
     9241 ttgattaaaa cgttttacat atccttcaac attgagcgat aaatgcaatg gcaaatccca
     9301 ttcgaaacct gcaattacat gccgcgcttt ttgaagattt gattttactt cttcaccatt
     9361 aaattcttcg ggtaaacttt cgggtgccga taaaaaacca taaaagaaat ttactatatc
     9421 tttatcgctt gtggcactga gcaaattttg cgaaaaggtt cccgcagcgg ctttcagcct
     9481 gaatttatcg aacatattat actttaccga aatgcgtggt tcgggcacag cctcgccaag
     9541 cgatgcataa tagtgcattc gaaatcccgg attgaggacc accttgctcc atactttttt
     9601 gtagttcaaa tatgcaccaa actctgtagt gttttcggcc tgctcaaatt tgtttccaag
     9661 taaattataa aacttaaaat ttgttttaaa gcctaatact tcaagcccat atttaaattc
     9721 atcattgctc gaaaaatatg taaagttgaa tgccatatta aaaccattgg tttcgctaag
     9781 gcgaggtttt tcatcggcct ctttcaattg catgctgtat ttggagtatg caaagttgcc
     9841 atcaatcaat gtttgcgaac ctccgggtac caccacaaac ttacttccaa aaccatttga
     9901 gccccaacta aatttcgaaa cctgcttgta attaacttca tcgttaaaat taaaaccaaa
     9961 taaacttatt ttgctgccgc tcacattgct ggtagaaact tttgcaaaaa tatcgcgaaa
    10021 cttaaatggc aatccatttt ctttatctac atagttgtaa aataattttg atgttttgtc
    10081 gaggtaacta tccttaatag aaaaaataaa tgatgcactt ccatcctctt ctgtttttgc
    10141 acgcttgagc ggaccttcaa ccaaaagctt actttgaaat gtattggtag aaatttttcc
    10201 tgacaggtgt ttcttattgc catcgcgcgt ggtaatgtcc attactgaag aaattcgccc
    10261 tccatacttc gaaggaaaag cacctgtata cacatcggta ttactaagta tatcagtatc
    10321 gaaaaccgaa aacaatccta tagaatgaaa agggttgtaa attatcattc catcgagcag
    10381 cactttgttc tgaactggag tgccaccacg aatatataat tgcccacctt gatcaccggt
    10441 aaaaatcact cccggtatca cttgtaaata ttgtgctaaa tcgggttcgc cccctataga
    10501 tggaagtcgt tttatgtcgc gaggtgtaat tttttgtacc cctacttttg tttccgattt
    10561 ctgttctgtt ttttctgccg atacttcaac tgttcttaaa tttacacttc gctttacaag
    10621 gaataatttt ttattcaatg tttcgcccat tactaatttt accggcaccg ataatgtatc
    10681 ccatcccata gcagtaactg taaggaaata atcacccggt ggaactttgt taattgaaaa
    10741 atatccgttc acatcagttt gagcaacata ttttgttcct ttcaaatata catttacaaa
    10801 caatactgcc tcgcccgatt ctttttcgta aacaaagcca cgcacaatgg cattttgtgc
    10861 ctgcgaaata tttatagaaa tggtaaatag aaaaaatata aaaagtaaaa tgtttttcat
    10921 aaagtaaatt tgtattctgt tcaaaattga cagtgcgcaa tatgaataaa aaaaatcgat
    10981 ccactatcag caaactgtaa tatttgatac cgcatttagc aatttaacat tgtaattatt
    11041 ttaatggaaa tggatgataa ataacaaagt tgcagcaaac atttttctta caaccaatat
    11101 atcaactact atattttcaa ttcgaaaaac ctatgcttca tacatttgtt gcttactttg
    11161 cgcacaattt ttattaaaaa ataaatatgg catacaagag cacacgcgat tttattgctg
    11221 cactggaaag tgccggtgag ttggtgagag taaaagaatt tgttgatccc aaactagaaa
    11281 ttacggaagt aatcgatcgc ctgtcaaaac acgatggccc ggcagtatta tttgaaaaca
    11341 ccggaacaga ttttccaatt ctgataaatg ccatggggag cgaaaaacgt atgagcatgg
    11401 cacttggcgt tgctcatctc gatgatattg caaaacaact ggagcaatta tttaaaacga
    11461 taacagcacc caaaaattcc tttctcgaaa agttgaaagt gttaccgcaa ttaggcgcca
    11521 tatcatcgtg gatgccaaag caaattagtg gacgtggtgc atgtcaggag gtggtaatgc
    11581 ctcagcccga tattacaaaa ctgcccgtaa tgacttgctg gcccgaagat ggcggcccgt
    11641 ttttaacctt gccaataatt caaactcttg acccggtaac aaatattcgt aatgtgggct
    11701 tgtaccgtat gcaagtattt gaacctacca taaccggaat gcactggcat aggcacaagg
    11761 tgagtgcgcg acattttaat gagtataaaa aactcaaccg tagaatgcct gtatcggtaa
    11821 cgcttggtgg cgacccttgc tatgcatatt gtgctactgc cccactaccc gatggtgttg
    11881 acgaatttat tcttgccgga tttattcgta aaaaaagagt ggaattggta aaatgcctga
    11941 ccaatgattt atacgtgccg gccgattccg attttgtaat agaagggtat gtagacccca
    12001 acgaagattt tattttggaa ggcccatttg gtgaccatac aggttattat tcgcttgccg
    12061 atttctatcc taagtttcac atcacttgca tcacacatcg caaagatgcc gtgtatccat
    12121 caaccattgt aggcatacct ccgcaagagg atgcgtggat tggcaaagca actgagcgta
    12181 tatttttggc gccaataaaa atgacgatgc ttcccgaaat tatcgacatg gttttacccg
    12241 ttgaaggtgt gtttcataat cttgttattg taaaaataca caaagaatat ccgggccatg
    12301 cgcttaaagt aatgaactcg ttatggggtg cggggcaaat gatgtttact aaaatgatga
    12361 ttgttaccga tggcgattgc aacatacacg atgtgatgag tattgcgcaa cacatatcga
    12421 aaaatgtaaa cgctgagtcg gatttttatt ttacccaagg ccctaccgat gtgctcgatc
    12481 atgcttgcac cagaatgtcg tttggtggta aagtgggtat tgatgctact ataaaatggg
    12541 atgaggaaaa gccattacac accaacacaa acaaacaatc cacgccaaat caatttgttg
    12601 aaataaaaaa tttattcccc gaaataacaa atgttaatgc caatttgctg gcatcggata
    12661 tttcgatggt atttataagt gtggaaaaaa atcgtaaagg ccacattgca gaattaagca
    12721 aacaattatt tgaactcgat gcatttgcca atgtaaatgt gctcatacta ctggaagcca
    12781 acatagatgt gagtgatata ggaaatgctg tgtggcgatt tgcaaacaat gtagacccgc
    12841 ggagagatca cataatggta aaacatcacc aaacaacttc ggtagctttt gatggcaccc
    12901 gtaaaacatt gatgtacgat gactttgaac gcgactggcc aaatattctt gtaagcgatg
    12961 atgatacaat tgcgaaggta gatgctatgt ggagcaaatt aaatttgggc gggttgattg
    13021 cttcaccatc gttaagatac cgcaatcagg tatataaagg tggggcagtt gtgaaggatt
    13081 aaagcttctt tcaacatctg tttgaaaata atttttattg gaaattctgc ttctttcaaa
    13141 atgcaatttg atgtaatttg taaaaatccc caaacaattt tttgatgcat tatttctatt
    13201 tcgggttgct ttagcaagtt tatgaaaagc aatctttttt agggaacatt atgccctcaa
    13261 aaaaaatgaa aagtgaaagc aaaatagttt gaaatcaacg caaaaaaatt gtgtgtgtcg
    13321 ggaaataaat tagttctaaa aaaaacttta caacgcccgc attttaaata gattttaaaa
    13381 tacctttgca tggttttttt acacattctc acaatctcag ggataaatat ttagtatggg
    13441 tttttttgat cttcttacgc aggaaatagc tattgactta ggtactgcga acacgctcat
    13501 tattcacaac gacaaagttg tagtagacga gccctcaata gtagccattg accgcaccac
    13561 cggaaaggtg ctggcagtag gcaaacaagc catgatgatg catggtaaaa cacacgaaaa
    13621 cataaaaacg gtacgcccgc ttaaagacgg agtgattgcc gattttgatg ctgccgaaca
    13681 catgattcgt ggcatgataa aaatgataaa tcccggcaaa cgattgtttc aaccttcact
    13741 caaaatggta atttgcattc caagcggaat taccgaagtt gaaaaacgtg ctgtacgcga
    13801 cagtgccgaa catgccggtg ccaaagaggt ttatcttatc catgagccta tggctgctgc
    13861 cattggtatt ggtatagatg tagaagagcc caacggaaat atgattattg atattggtgg
    13921 tggtaccagc gaaattgctg taatagcact tggcggtatc gtttgcgacc aatcgatacg
    13981 ggtggcaggc gatggtttca cctccgatat ttgggattat atgcgcagac aacacaacat
    14041 tcttattggc gagcgcagtg ccgaaagaat aaaaattgaa gtaggaagtg ctttacctga
    14101 attggacaat cctcctgccg attttgccat tcatggacgc gatttgatga atggaattcc
    14161 taaggaaata accgtttcat ataatgaagt tgctcatgca ttggacaagt cgatttcgaa
    14221 aattgaagaa gccattttga aagcccttga aatgacacca cccgaattaa gtgccgatat
    14281 ttatcgcacc ggaatttacc tcaccggtgg tggtgcatta ttacgcggat tggataaacg
    14341 catctccatg aaaacaaaat taccagtgca cattgccgaa gatccacttc gtgccgtagt
    14401 acgtggtacc ggaattgcac tcaaaaatat cggacgttat aaattcttaa ttccgtaatt
    14461 cttgttgggg aagcttgaag tttgaagttt gaagtttaga gtttgaagtt taaagtttgt
    14521 agtttgaagt ttaagataaa actggaatta gaaaattcaa agcttttata caattcaatt
    14581 tttttttgat tgaaagcaaa tgaaataatt ttaaagacaa tagatttaag aaaaaagcac
    14641 tagtgaatat tttcgaacgg tcaaaaaaaa agcaacactc accgcaaaca aaaacaccaa
    14701 tgcttgcatc ccgtaagcgg tttcagggct aaaaactaaa aactgaattt cgtaaccaag
    14761 aactaagaac aaggctcacc gcaaacgtaa ctaagaacta tgaacaaggc gtatcgctaa
    14821 cgaaactaag aactaagaac aaggcgtatc gcaaacgaaa ctaagaacta agaacaaggc
    14881 tcaccgcaaa cgtaaataag aactaagaac aagtctcacc gcaaacgaaa ctaagaacta
    14941 agaacaaggc tcaccgctaa cgtaactaag aactaagaac aaggcgtaac gcatacgtaa
    15001 ctaagaacta agaacaaggc tcacgataaa cgaaactaaa aactccccca actaataacg
    15061 agaatttcga atgcgcaatc tgttacaact tttatggaaa aataactttc tcatactatt
    15121 tgtattgctc gaaattcttt gtctttatat gatagttcaa aaccaatatt atcagcgtgc
    15181 tgtttttatt aattcatcca ataatgtggt gggaaacgtg ctttcaaaaa taaattctgt
    15241 gaagcagtat ttattcctca cacaaaaaaa tacggagttg caacatcaaa atgcaaagtt
    15301 gcttgcaagt gacagcacat cgaaattttc attaacaacc gtcaaagaca cctcgcataa
    15361 caaagacctt atacaacagt atacatacac cgttgcaaag gtgattaaca acagcgtgca
    15421 tcacagcaat aattatctca cactcgacaa aggttcgctc aatggcattg aacgcgatat
    15481 gggtgtggtt gggcctcttg gtgtagttgg tgtggtaaaa gaagtatcgc ctcatttttg
    15541 cactgtactt tctttgttgc acaaagagtg gaaaataaat ggcaaattaa aaaaatcggg
    15601 agagtttggt cctgttgcat ggaacggtaa acctttgtat gcaaatctcg aagaaatacc
    15661 taccagtgca cgaatcacaa aaggcgatac aattgtgaca acttctttca gcacaatatt
    15721 tcccgaaggc attcatttgg gatatatcac ttcatcgaaa attgaaccgg caaagagtac
    15781 gctttcaatt gagatgaaat tagcaactaa tttttattct gtaggtgagg tttatattat
    15841 taccaataga ttgaaaactg agcaaaaaga attagaacat aaaacagatt ccactcatgt
    15901 tgattgaaat attattgatc attgttcgat ttattttgtt ggtcacatta caggtggtag
    15961 tgctcgacaa tgtgcaatta agttatttta taaatccata tttatacgta ttgttcattt
    16021 tgttgttacc cataaatatg cctcgtggtc ttgttcttat aattgcatta atacttggtt
    16081 ttgtagttgg tgcacctacc aacacatcgg gtattcacgg agcggcttgt gtttttattg
    16141 gctacatgcg tccatttgta ttaaaattaa ttgcaccccg cgaaggttat gaatcggatg
    16201 caaaacctac tatgcgcgaa atgggattaa actggttttt ttcttatgcg gctatcatgg
    16261 tttttttgca ccatactgtt ttgttttttc tcgaagtatt tcgcttaagc gaatttttta
    16321 ccaccatcat gcgcattgta gtaagttcat tgtttacact ggcgtttatc atgatatcag
    16381 tttatttgtt ttataaacca aaagggcaac gatgatacgc gaaaccggtc agcaaaatgt
    16441 tattcttgga atttttatca ccatcatatt ggtgtatata atgcggttat tttatgtgca
    16501 ggtagttgat gatgagtata ccgcttcggc caataataat gtattgcgtt atgttaccgt
    16561 atatccaccg cgtggagtaa tttacgatcg caagggacac gtgcttgtac acaatgaagt
    16621 gttttacgat ttgatggtaa taccacgtca ggctaaaaat attgactcaa cagaattttg
    16681 cagcttactc ggaattagca ttgaagattt ttcagcacga atggctgcag caaaaaaata
    16741 ttcttctata aaacctacag tatttgaaaa acaaatttcg aaaataaatt tctccaccct
    16801 tcaagaaaag ctttataagt ttcccggctt ttatgctgaa ccacgtacac tgcgatacta
    16861 cccaaaacct gcagcagccc atcttttggg ttatgtgggt gaggtagacg aacgcctgat
    16921 tgctaaaaat gcttattaca aaatgggcga ttatattggc ataagtggta tagaacagag
    16981 ttacgaaaaa gaactgcgcg gacaaaaagg attgcgcatt atgatggtgg atgtgcataa
    17041 ccgcgagaaa ggaagttttc gtgatggtgc attggatacg gcatcagtcg aaggtcaaaa
    17101 tcttgttctg tcactcgatg gcgatatgca attatatgcc gagcgactga tgcaaaataa
    17161 aataggcggt atagttgcta ttgaaccggc cacaggcgaa atacttgctg tggtatcttc
    17221 accatcatac gatcccaatt tgttagtagg gcgtgtgcgg gcaaaaaatt atgtgcaact
    17281 tttacaaaac aatttgaagc cactttttaa tcgtgccatg caggcttatt atccaccggg
    17341 ttcaactttc aaattgataa atgcacttat agcttttaac gaaggattta ttaatggcga
    17401 aacgcggcag acctgttctt tcagggtggg cagtcattcc atacattgcc atccacatcc
    17461 aggccctgcc gatttacgaa cagcagtgca gtattcgtgc aatccatatt tctgtaaaac
    17521 atttgttaat tatgttgaaa atgataaatg ggtaaacacc gaagatggct atgaacactg
    17581 gcgcaaacat gttttaagtt ttggtgtagg acgtaagctg ggtatcgata ttccacatga
    17641 attgccggga cttgtgccta cagtaaaata ttacgataaa tatttcggca aaaatcactg
    17701 gagcgcaaca acagtttttt cactcggcat aggtcagggt gaacttggaa ttaccccaat
    17761 gcaaatggca aatatcgttt gtataattgc caacaaaggc tattataaaa tcccccatgt
    17821 ggtaaagtat attggcaacg aaaaaaatgt tcccattgag tttacaaaaa agaatttttg
    17881 cgatgtgcaa ggagaagctt acgatgttgt aatagatgga atgcagcgtg tagttgaggc
    17941 cggtactgct cgtgtagcga gattagacag cataattgtt tgcggcaaaa caggtacagc
    18001 acaaaacccg catggaaaag aacatgcgtt gtttgtttgt tttgcaccac gtgtaaatcc
    18061 taaaatagct atcgcagtta tggtagaaaa tgccggatac ggaggtacat gggctgcacc
    18121 tattgccagc ctgatgatgg aaaaatattt gaccgatact ataaagcgcc ccgaccttga
    18181 gaaaaaaata ctcaacgctg taatattgcc aactccaaaa gttattcccg attcgttaca
    18241 aattccagac tgatgcgcca acaaaaaagc atattcgata atatagactg gactacagtt
    18301 ttaatatatc ttgcattagt aatttttggc tggataaata tatatgccgc tgtttacgat
    18361 gaacgtttcc agagtatttt cgataccact caacgttacg gaaaacaaat gttgtggata
    18421 gtgcttgcca taataatttc gctcgctata ttagtagtag atggaaaatt ttacgaagca
    18481 tttgcatact atatctacgg atttttcatg ctggctttat tgctggtatt attaatagca
    18541 cgcgatgtaa aaggtgccgt atcgtggatc gatattggaa gtttcaaatt gcaaccatcg
    18601 gaatttacaa aatttggcac ctgccttgcg ctggcaaaat ttctcagtga accttatacg
    18661 cgcatgaccg aaaataaaac caaaataatt gccgccttga tttgccttgt gcctatggca
    18721 ttgatactga tgcaaaacga taccggttcg gcaatggttt atgcatcatt tatttttgtg
    18781 atgtatcgcg cgggtttgtc acaaaatttt ctcatcatag gttttataac catcctcatt
    18841 tttgtgttgg cgcttatgat tcccaaatta gttttggtag gagttattat tggtgtaagc
    18901 gcattaggcg cactgttttt aaggcgcacc atgaaaaata ttttgatgct tgcatttatt
    18961 gcaatagtgg gttgcgctat ggtgtatggc gtagattatg ttttcaatga agtattacaa
    19021 acgcatcagc gcgaacgtat agaggtactg ttaggcaaac gtgccgaccc gaaaggtgcc
    19081 ggctataatg tgaaccaaag tttgatagca attggttcgg gagggcttac cggtaaaggg
    19141 tttttgcaag gcacacaaac caaatatgac tttgttccgg aacaggctac agattttatt
    19201 ttttgtaccg tgggcgaaga atggggtttt attggcacta ccatattaat ggcaatgttt
    19261 ctttggttac tcattcgcat agtgatgatt gccgaacggc agaaaaataa ttttgttcgt
    19321 atgtatggct atggcgtggc gtcaataatt ttcttccata tttttatcaa tattggtatg
    19381 acaataggtc tattgccggt tattggcatc ccgctgcctt tctttagtta tggcggttct
    19441 tcgttgtggt cgtttactat tttacttttt atacttataa agctggatag ctaccgcatg
    19501 tttgtattgc gctaaataat tcttttgctt ttccttcaac atcattattc aaatcatttt
    19561 attgtaattc gaatcgaaag tacgttgctc gataaaaccc gcttatgaat aaatgattca
    19621 gggtttttaa catcatgaat catattattg taagtgctct atcgaaaatc tattattaat
    19681 ttacgatctg cgctattgat atcaaatgct aattctttca aattttaaaa gcaagatctt
    19741 agatcactaa accaacaatt ttcattggca aaaaatattt atattgcccc aaattgaaaa
    19801 tatcgcatgc tcttcaactc ccttagtttt gcagtttttc ttccggttgt ttttgtgctt
    19861 tactggtttg tggcaaaaaa taaattagtg tggcaaaatt tgctcttgct attttcgagc
    19921 tacttttttt atgcctgttg ggattatcga tttttattcc tgttaatatt ttcaacgctg
    19981 ctcgattact taaccggttt aaaaatgcac caaaccacca acacaaaaat gcgcaagttt
    20041 tggttttggc taagtgtaat aatcaatctt gggtttctgg cagtgtttaa atattataat
    20101 ttttttgccg atacatttgt tacagctatt tccaaacttg gtttacatgc cgaactctcg
    20161 accatacaag ttatactgcc ggtaggcatt tcgttttata cgtttcatgg tttatcgtat
    20221 gtgatagata tttatcgcaa gaaaatagag ccgacaagaa actttattca ttacactgtt
    20281 tttgtaagct tttttccgct tctcgttgcc gggcctatag aaagagccac tcatctttta
    20341 ccacaaataa aagtgttgcg cactttcaat tatcactatg cagtagatgg gttgcggcaa
    20401 atattatggg gcttgtttaa aaaaattgtg atagcagata actgtgcaca gtttgcaaat
    20461 attattttca acgactccga ttcgtaccat ggtacaactt taatattggg tgcattgtgt
    20521 tttacgtttc aaatttactg cgacttttcg ggctatagcg atattgcatt gggtacagcc
    20581 cggttgtttg gcattgagtt gttgcgcaat tttgccttcc cttacttttc gcgcgatata
    20641 gcagaatttt ggaggcgctg gcacatatct ctttctagtt ggtttcgcga ttatctttat
    20701 attccattgg gcggcagcag cggtggcatg tggatgcgga tacgaaatac atttattatt
    20761 tttattgtaa gcggattttg gcatggtgcc aactggactt ttattttttg gggattttta
    20821 aatgcatgtt attttttgcc tttgcttttg gcagataaaa atcgcaccaa tttggaaatt
    20881 gtagcaatgg ataaatggtt gccatcatgg aaagaatttt tgcaaatatc gataacattt
    20941 tcgctcaccg tttttgcatg gattttcttt cgcgcacaaa atatgcatca tgcatttact
    21001 ttcatacaag gaatagttga aggcctggca cacaaaagtg cttacataga aacgctaaat
    21061 tttttacggt ggaacatcgg gaagtcgatt ccaatcctgg taataatttt tatgctggta
    21121 gaatggatag gtcgctcgca acaatttgcc attcaaaatt taggcttgca atggccacgc
    21181 actgtgcgat ggggcttgta ttacattatc attatagcca tttttatttt tggcggcagc
    21241 gaacaacaat ttatttattt tcaattttaa tgatgtcaaa atttattcgc accctacttt
    21301 catttatcgc catcgttgcc attccatttt gcattttggt ggggctgtat ttttacttcg
    21361 atccttttat ggtggtgcgg aattataaaa atttctctca ttcgcatgtg gcactcaatc
    21421 gcgattatgt aagctcacaa atatttctgc aaaattatcc taagcataaa ttcgactcct
    21481 atatttttgg cagttcacgc actatggcat ttgagcccaa tgtatggaaa accaagctgg
    21541 aggccgatgc ccggccattc atgttcgatg ctgctgctga aagcatttat ggaattcatt
    21601 cgaaaataaa atacctcgat gatcacaaca ttcccattcg ctatgctttg attatagttt
    21661 gtcacgatcg cacttttgaa cattcgaccg atgaggcggg ttttttattt attaaacatt
    21721 acaaattttc gaaaaacggt tttataaatt ttcataaaac ttttttttta gcatatctcg
    21781 atcctcattt tttaaaagcc tacttcgctt atcaaaacaa aaaaaaatat gaaaaatgga
    21841 tggcaaaata tatggtggag tgggatattg aatacgacac tgtcaataat caaatgtttt
    21901 attacaagga tatcgaaaag caattgctca ccgatcctga tgcatattac aacacaagaa
    21961 tgaaaaattt ctttgccccg caaaaaaaag aaaccataga ttctaaaaat ttgattactg
    22021 cctcacaaat aaaaatgatg aaagagataa tagcaatttt cgaaaagaac aaaacaaagt
    22081 ataaagttgt tgtaagtcct ttatacacaa aagtaaaatt ctcgcatggc gatacaaaag
    22141 tattacaatc aattttcgga aagcagtttt atgatttttc gggcgacaat atttatacgt
    22201 caaccaaata taattattat gaaatgagcc attatagaaa aacagctggc agacaaatta
    22261 tggatagcat ttaccatccg tagatatttt ataacctaca aaaaaaagag tattagtttt
    22321 tcaaagatgg gatgtatcct tcatcatgta caacatcttc ctgcatgtca cgggcttttt
    22381 tcaaacgtgc catttcattt atcatgtata acaaacacat gtaaaatgga atcatgggaa
    22441 ttttataacg tacaagactt ccaaaattgg aggtggtaat tcctaccgag aaactaaaaa
    22501 acatgctgaa agtaaaacag aagatcaaca taggttcgct aaaaatataa cggaaaaaat
    22561 tgatgacttt agtgcgccat aacacttgta aggtgaaaaa taatatccac agattttcga
    22621 tacttgaagc aagcattaca ggattgtttg cttcgagaat aaaaggccgg aacagcgaag
    22681 cattgattgc caatggtgct ttagctaaca tacttgataa tgtagcatca taatcgccaa
    22741 tatcaaaagt gttgccctga taataatcgc gctttaaatc ttgctgcgtg tctaccgcct
    22801 tttcaagtat actgtcgagt gaataggcgc ttagcgaatc gctcataaat aaaagtacac
    22861 caaagccgcc acctatcatc actatcaaaa gaattggaag aaaaaatgca cgcatcattg
    22921 ttcccctgat tttgccaagg gtaacgccta aaagccaaat aagtgtaccc ggcaatatgc
    22981 caacaaatat gtatggctta atggataaga taagataaac cgacacaaaa agtagcaaca
    23041 cattggcaaa tattttttgc cttttaaaaa atatctgata aatagaaaat acaaaatatc
    23101 ctgttgcact tagagtgata gtgtctttca aaattcccga accccaaaaa ataactgatg
    23161 gcacaaaaaa tatggccata gcaaattgct tttcaagttt tggaaactga tctatcatta
    23221 cttcaaacaa tttccacaca ccattgaatg agaaccaacc gattataagt gaactcagca
    23281 caaaagaatc gaagcaaaaa aagattaccg gtgaaattaa tcgcaccaca aaaaatgccg
    23341 gaggatcgcc aagataatac ggatagttgg tatcgttatc aaaataaaac cattggtcct
    23401 tgcccgcata atgaaacata acatcataga aatacgaagg atttttcaac aacaaatact
    23461 tcatacaaag cgcattaaaa aaataacccg ttgtatcacc accgccataa tacaaacaat
    23521 acaccaggca caagcttacc ccaccaatac atttcatcat cacaccgggc acataatatt
    23581 tgtaataagg atacttttct actttacctt ttgcataaaa cctcgatata aaatatatca
    23641 gtataaaaaa aaccggggtg gtgaataaat cgagaatact taaatattgc atgggctaat
    23701 tgtaacgggc aataatatcg aaatgtttat tttcaaagtt gtaagaggcc gtaagctgtt
    23761 cattcgtaat ggcatcatat attttataat tccatgatat aagcaactcg aataatgcct
    23821 tagccgaatt gccttgccgg tgaagattac tgtcatttat ttctacaaac aaaacaggat
    23881 gaaatttatt tatagattct ttgcctccgc ttagtatatt atactcaaag ccttcggtat
    23941 ctatttttat caaatctatt tttgcaatgg agttattgtt tacaaaagta tcgagcgaca
    24001 atgcttgcac tttttgatct ggcgatgaaa tatcaccttt aatcatatac accgaaccac
    24061 tgttttttaa tgttggctga tgaaagtgca atacctcttc cctatcggac aatgcaacgt
    24121 tgttcaaaga aatatttgtg aaagaattat tactgatatt ttgcaatgcc ctttggtaga
    24181 gatgcggaac aggttcgaaa ccataatttt tcccctgaga atttatgcgg gcaaagttca
    24241 taagggtttc gccaatgttg gttcctatat cgaaaatagt ttgcccttgt tttgctaagt
    24301 catataaaat atggcgcggt tcattttgca gtccataata aatgcaatac tccatataat
    24361 cgcttatatc cagtttgtaa tttattccat tgcgtgttgc cgttcgccat gtgggttttc
    24421 catattgata attgttgggt attatttttg ccacaaatgc atcagtgggt ttttgctgtg
    24481 tgtgggtaac aataatattt tcaagaaatt taatcttaaa aatatttctg aaaaaattaa
    24541 ggaaacttgt tttggcagag gtttgaagtg tatcgcccaa gtcattatta ttcatatggc
    24601 aaatttaacg agatattatt ttaaaaacaa tgttttttgc accttagcca accatataaa
    24661 aacatatgac agattttata ccatttaata agccttacaa agcagggcga gagtttgagt
    24721 atataagcga ggctatcaat cgtggtcacc tttcgggcga tggatatttt acacgcaagt
    24781 gccaaagctg gtttgagcaa acctataaat tcaacaaaac attgctcacc acttcttgta
    24841 ccgatgcttt ggaaatgtgt gctctattag ccaacacaaa ccccggtgac gaaattatag
    24901 caccttctta tacttttgtt tccactgtaa atgcatttgt attgcgtggt gctaaaatta
    24961 tttttgccga ttcatcgcct ttgcatccaa acattgacga aacgcaaatt gaaaaactta
    25021 ttaccccaaa aaccaaagcc atagtggtgg tgcactatgc cggagttgca tgtaatatga
    25081 atcccatttt agacctggcc cgcaaatata atttgtacgt gattgaagat gccgcacagg
    25141 ctatagattc tttttattat gataagccgc ttggtggcat tggcgacatg gcagcatttt
    25201 cgtttcacga aacaaaaaat attatttgtg gcgaaggtgg catgatagtc atcaataata
    25261 aacaatttga aaagcgggcc gaaattattc gtgagaaagg aacaaaccgt tcatcttttt
    25321 tccgtggcga agtcgataaa tatggatggg ttgatgttgg cagttcattc ctgcccagcg
    25381 aattgcttgc agcttatttg tatgcacaaa tagaagcaat aaccgacata caaaaaaaac
    25441 gtatgcacat atggaaaaaa tattttgatg gtttagcttc acttacacaa tccgaaaaaa
    25501 taaaattccc ttttatgcca acatatgcca ccaacaatgc acatatgttt tatattgaat
    25561 gccgcaatct tgatgagcga acagctttgg taaagtattt aaaatctcaa aatattcttg
    25621 cggtgtttca ttatttatcg ttacacaaat ccccttacta tgcatcgcaa tataatggcg
    25681 atgatttacc aaatgccgat aggtttactg attgtattct tcgtttgcca ttgtattatg
    25741 agctcaccga ttcgcaatta gaatatatca ttcatcacat tcacgttttt tttaatgaca
    25801 attaaaaaat gaagtattgt tcatgaacgg tatcatcaca caatcgaact atataccctg
    25861 gaaaggatat tttgataata ttgcacaatg caatgtgatg gttgtttatg atgatatgca
    25921 atacacaaaa cgtgactggc gcaaccgcaa ttatataaaa acaccacaag ggttaaagtg
    25981 gctaaccata ccggtagaag tgagcggaaa atatttgcaa aaaataaacg aaacaaaaat
    26041 ttcgaacaac gaatggaata aagaacattg ggcaatgcta aaacaatatt atcgcgatgc
    26101 accctatttc agcgaatttg caacatgggt agaaggcctt tataacgatg caatatttaa
    26161 tactttgaca gaaataaatg ttcactttct gaaagcggtt tgtaaatttt tagaaataga
    26221 aacccaattt atcgatagtc gccaatttac gctcgttgaa ggaaaaaccg aaaggcttgt
    26281 ggatatttgc aaaaaatgcg gcattacaaa ttactactcg gggccagctg caaaaaatta
    26341 tatggacgaa aatttatttc tcaacgaaaa tatcactgtg cattatgccg actatagtgg
    26401 gtacaaagaa tacccgcaat tattttctcc gtttgaacat ggagtatgca tttgggattt
    26461 aatattcaat gtgggaaata acagtaaaaa gtatttaaaa aatattgtat gagccaacct
    26521 gttgaattga gtatagttac tacagtttat aaatcggaaa aatatcttcc tgaatttatc
    26581 gcactcattt tgcaatcgat caaagaaatc ggcattaata attttgaatt actttttgtg
    26641 ctcgatggaa ttaccgatgc ttcgaaggag tatttactgg cacgaaaaaa agatattcca
    26701 caaatagttg tagtagagtt gtcgcgcaat tttgggcatc actatgctgc ttctgcaggg
    26761 ttgaatactg caagtggtga tttggttttt cttattgact gcgacctaga agtttcgcca
    26821 acagtactta ctgcgttttt ccatgaacta aaaaagcaat cgtgtgatgt tgtatacgga
    26881 gtgcaaaaaa tgcgtaaagg cggttttacc gaacgttttt ttggaggctt gttttggaaa
    26941 atgataaatg cactgagtga tacaaaaatc cctcacaaca ttgttaccga aagattgatg
    27001 acacgcaact acatcgatgc tctaaactct atgggcgata aaaatttgtt tatggctggt
    27061 ttgatgtatt gggcaggatt caatcaagta ggcgtgcctg tagataaaaa acaacgcacc
    27121 ggacaaagtt cttattcgtt cggcaagcgt atcagtcttg ttttcgaagc ggtgacttct
    27181 ttttctgaac gtccgctaaa aatattattt aagattgggt tatacattac agcgctttct
    27241 gttattgcat cggtgtatct tttaatcaaa aaaattcttt atcccaatac tatatttatg
    27301 ggctacactt ctgtatttac actcatacta ttttcgcttg gaatgcttat ttcttcaata
    27361 ggaattgtag gcatatattt atcgcgcatt tttaaacaag tgcaaaaccg tccgctattt
    27421 attattaaaa aaatatatta attcaaagat ggaaaatctt gattcataca aagaagcata
    27481 taaccaaagt ttcaattttt acgatgaaaa ccaatggtat cttgcgcagt atgcaaaata
    27541 tttgtgtgca aaaattactg ataccaaagc ctcatccatt ttaagtttag gtattggcca
    27601 tagtgtggta agcaacagca ttattcaact attaaataca ggtaatatac aatatgacat
    27661 tgtagaaggt tcgcccgata ttattaataa attcaatgcc acgtatcata acatatcgat
    27721 gaaagtgtat gaaagttatt tcgaaacatt tacacccgat aaaaaatacg atgccattga
    27781 aatgggtttt gtgctcgaac atgttgacga ccctttgctg gtgttaaaaa agtttaaaaa
    27841 ttttctcaat gataacggca ccatgtttat tgccgtgccc aatgcaaaat ctttgcaccg
    27901 gttaattggg catcatgccg gattgttgcc cgatatgtac aaacttagta agtacgattt
    27961 ggaacttggg cataagcggt atttcgattt ggatagcatt accaatttgg ctaccgaagc
    28021 gggtttgcgc gtaaccatgc agcgcggcct tatgcttaaa cctattaccg gagagcaaat
    28081 gaaaaagctc gactggcaaa aaaatattat cgatgcgcta ttcatagttg gcgaacatta
    28141 tccggaaatt tcaaattgca tttacctcga agcagtaata aataaatgaa tgtactagta
    28201 acatgtggag gcggattgca aggattaact ttatacaaag agttaaatgc attgcaatcg
    28261 aaatttaata ttacgtctca cttattcgac attcatgccg aaaacatttc gaagtatttt
    28321 ttcgattatt tttttcaatg ccctcctgtt gccgattccg aaaaatatat ttctgcatta
    28381 ttcgattatt gcaccaatca caaaattgat tttatttttc ctgccacaac ttatgatttg
    28441 ctttt
//
LOCUS       DAORQA010000017        26703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_429_length_26703_cov_43.379053, whole genome shotgun sequence.
ACCESSION   DAORQA010000017 DAORQA010000000
VERSION     DAORQA010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 26703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26703
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_429_length_26703_cov_43.379053"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PK736_02720"
     CDS             complement(<1..346)
                     /locus_tag="PK736_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32337.1"
                     /translation="MKEVVDSLNNGKNSFALNKLNLIVDQNVIEYNKKYIATLVALNY
                     DASLDADSDTIASILNIALMHPFYGGEAVYWARAILHLNIIDVMPPQRKGKNVINDAM
                     LNTIKTKGTLYPN"
     gene            complement(362..4204)
                     /locus_tag="PK736_02725"
     CDS             complement(362..4204)
                     /locus_tag="PK736_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32338.1"
                     /translation="MTANEDAINNLIGINLPQMGIQRDVYCMQVIEINNNRNLDKFLV
                     GTDVGLYHFTYNDVTSSTITALNNLVPNPNNSGIPYHVTSIQQYSSNHYYVVIHGQGI
                     YDYNSSSNTWATGANLVTSIFARNNSNNIIYDVTTNSTVPNYTQLHFIRNNTNDITGY
                     LLMNERPTVAYGSPANVYVGILYSPAASFNGPPTGIFTALTVDASDPANEFGWNTTRP
                     HANINSSCMVQGTLNRNYLIVGKNGNIFASKADVQGAAVNWQQIYTEIVTNACGTDVA
                     YGNRGFVNTASQSIFHIGTELYYSERDRLMSFSNLPGFTAMLDVTNTTLCRNIAFIPA
                     VSGCGNTSANLALTDCFSITNNTFPLSNDLNLYAGAAEGPGEQMGRGTIIMLPAGTTS
                     WTNAVTNTVCGDPIKILFSSGNDMYALVTTIAGPRELFHFTLGGTLNLCTVGSITSIR
                     DVVLSPDGNTMFAIPFASNDNVIQLTKSSATAWSPVGAPLDLNGNTANADVIAEELIT
                     YNYDLGYKVLLACSPRNANTHSKLVEVYNSDVGALVPIEINNDCNGNVPADDIFIDLF
                     DGDQNDRGITALAVAENIRTIYVAATQNEGTGPRARVYKGIYDINTGTIEPCWTDITG
                     NMPNKTVQCMGTNGSDCDNEFLYACVRGMGEWRWQVTPFTTTSGTVQNEFLRLTNDLN
                     LAGQLILENCTLSVSQGVSIICGQGDVLKLTNCYIYACGDMWQGIINNGGTVIIENST
                     IEDAILATRADGNGSKVQLSNVTFDHNFGGVSLNNGDYFYSYIRGCTFDCNDGQITKA
                     PHVAELTDNHVVLNNVARFTLGVNILPQNIFRNAVTAVLTNQSNIIIQNNLFDWQKLY
                     NFNSTHDAQAISLNGSYSNINLSPHTHTIGGLSNGNTFINWQKNIFARGCINLNIENN
                     IINMGQFDRSNEGVQESACKNIIIQNNAINRCKQGVQMFNNVTAVATVHANSFNKYFQ
                     LEEDFYDDEAIRCEYVLFDTYTPYTITDNEIFNVAIGIHIRNVNTTVNNNQYTTELSA
                     FFIDFYYNNHNAGSVSPHIGVWAENCNGATIFQNHATWTSAITVQEERDMIQGIRLQS
                     SLSCRLQENTMTNMGAGIDVFDDCSNSSLLCNEMENCWNGVYLDDASPTSFLSTQGIF
                     GATQATSTTWDNKWINNIGGFKIDGATWLQQNITWIYDPALSSEYDPDPSAGFVTRQP
                     GDLHNCYTPPPINDGERARIFGSAVGDSTATDGYDSLEFVIIDKEKFFKSATTNPAIL
                     ALGTNNDAKYIAEYDSLK"
     gene            4231..4398
                     /locus_tag="PK736_02730"
     CDS             4231..4398
                     /locus_tag="PK736_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32339.1"
                     /translation="MKLVINDMFGCIINSQEMLNERQKLNVTNYAQSIYYMHVYVNGI
                     DCEVHRFTVIK"
     gene            4426..6033
                     /locus_tag="PK736_02735"
     CDS             4426..6033
                     /locus_tag="PK736_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32340.1"
                     /translation="MLSFFIYKSNEKIYFNNPFLFFFSTVYSQNQNNAWVFGDSCGID
                     FTNTQNPTPIITNMVGRGSCVSICDTNGILMLYAYTVESSGALSTIVRNGLHQKLNQN
                     NTITGVAWYNELALFPKPGTQQNLYYLFSVGLNGAPNPGLYHTTIDMNLNNGLGGIVQ
                     QNIQIDNIEYGDCLTCVRHGNGRDWWVMAKINNFPFTFYNRFRTRLVTADSIYPAMIQ
                     DFNDASDGSFEKICWHPSYNKFMMIDVRGYMSEFDFDRCTGIITQVRTIFAEQLSNFS
                     NLFWEGAYSPNGNVFYVSRTSYGGNFGDYNYLLQYDLTAIDIPASCDTLDSTIYPQAD
                     CGAVRLAPDGKIYYSQAYPWGFPYQDSMHNVVNENLGVVNFPDSVGKACNFLPFSFYL
                     GGKRTYYGLPNNPDYSLGPLQGSPCDTLQWVGTPETPNLLQGGFRSLRINPNPANNSF
                     YIKYDIPTNENLLFVLYDSYGKEVLHKNLYGTFKNLLVHTEQLNNGIYFWRAVKDYNT
                     KTQRSNGKSPEGDLGVGAQNGKIVILH"
     gene            complement(6212..7792)
                     /locus_tag="PK736_02740"
     CDS             complement(6212..7792)
                     /locus_tag="PK736_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32341.1"
                     /translation="MAQDWNGNSYKSSTADTVFTVGNLSTIDASGNLFLAGQSRCIST
                     AANGQPCIRKISSAGVFQKQIYLGYSTSPFDDVIKDIKVYNNSLYVVMDGQYNFPPGD
                     HDILIYKYDLNLNLKWSSIYNGIGDLEDKGVKIIKGNAGSLLVLGNSADSICVLKLDS
                     ASGNLLKKRNFKNSNLDDSNTGVDIRYLNNATYVAGTTDMGPFQGSTVLLKMDNNLNF
                     VFTIIKKASIFGAIVQRDVVKTLQLDASGNCFIAGHIFSNAASPKPRPFLLKYNSSGV
                     LQYLKRYSYDDNECFDFFAESTGGLIMYTNKNKYLRISNATGIVTLGTTILTSLNFKV
                     ADVAKGADNNLYVFGRISYTQNFNGNPFEYRGVQVSGISTAGVTSVIYQENYQTPFRL
                     DLTYTPKRISVRNSNQVYFSMDVDDLSLLPNEFFVKYGRRQFTPARLAFESVENMGST
                     LSVYPNPAQSFINVALPTTITQGTIQIFDLQGKLMLTKNIDDTFQNEINIESLHTGAY
                     ILKLISNEFVVTDKFVKI"
     gene            complement(8198..11464)
                     /locus_tag="PK736_02745"
     CDS             complement(8198..11464)
                     /locus_tag="PK736_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC32342.1"
                     /translation="MISNQTMHDEPSSHNVFEVIKQRLDTAGADVMVHMNDTKLPLQA
                     LDFWSDALATELYNKGINEGDFVALYLPPGFALCAATMALSKLGANQMLFRDNLKPDF
                     LQQQADSAAFNCLITTLPNAADLFPKIILVDPNTSNDANAVTDSEEMFGGGHFFVEEF
                     NGENGASTKYTTFESSELFVDDKIVEYSDSLLYRLWHSIINNNKVELNKLDMPFWDQQ
                     IIEHIQGMDLNFGNEDDDVDLDVEDDADEIENQQNSDIDDLMKMDEEISFVENELEDN
                     ALWLDNMTDQNTSSINKGEVINLELPFIENITAEIENNVMEENIISESAKEDNLSFEI
                     NTFALENFGATDNQTFESDNQTVIDHSIENKNNTLINNTTIMGRQQHLVTELKEILEE
                     ASGIEMASADLNSSLIELGLDSLFLTQVSITLSKKYQTKITFRQLNEELSSISALATF
                     IDNHLPASVTYSEPAAVTNIEPTPALPAPQQMQMPVQPQATYTPTVNADAGSVEWLIT
                     QQMQIMQQQLALLSGMPVQAPQQSMAAPSAPVASPIVETPATEAKTATETKTEEPKEE
                     AKPFGAIARIEKNVSTTLNPEQQKWFNDFVKRYVALTQKSKTFTQLHRAHLADPRVVT
                     GFKPYNKEITYQVVVNKSKGPRVWDIDGNEYVDVLNGFGSNMFGHSPQFVVDALTKQL
                     EQGYEVGPQHPLAGELTQLICELTGSDRAALCNTGSEAVLGALRMARTVTGKNLVVAF
                     NNSYHGINDEVIVRGTKKLKSMPASAGIMPGAVENMLILDYGTDETLQIIKQHLHELA
                     AVLVEPVQSRMADFQPKEFITQLRAMTQEADVALIFDEVITGFRYAQGGAQEFYGVKA
                     DIATYGKVCGGGMPIGAMAGTKKYMDALDGGMWQYGDASVPEAGVTYFAGTFVRHPFA
                     LAAGVASLQHLKTQGPQLQQQLNAKTKGMVEQLNAFCYALDLPIAFTNFASLFKMKYK
                     HEYPFSEFLFHLWRMNGVHVLDGFPCFLTTSHTEADVQFIVDAFKKSVNELIALDLIP
                     HKKTTNGQAKTEPVAVTKNVDVSKPPVPNARLGKDPQGNPAWFVADPLRPGKFMMVQQ
                     "
     gene            complement(11611..12837)
                     /locus_tag="PK736_02750"
     CDS             complement(11611..12837)
                     /locus_tag="PK736_02750"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRC32343.1"
                     /translation="MNKILSTNILIFLLSIVACSSKKNVSESNEKNKNVNGIEKSTVN
                     NSDENITPKNNDTPLSPTKPTNGIQMPTGIYITSSAIDAGFSPSVVSNEDIEGIYLRL
                     SWNSIEPEDDKYNWSFIDQQIAQAIQYNKKVVIGIVSGRYTPEWVFGAGVPKLQFSEI
                     NHRGKGKKAFSVAVAPPWDSKYQSFILDMQTSLRDHLKAKPGFYDALIMVKFTGMNIN
                     TNEMRLPSQDKVTNGKDVTSDAIVIWQSAGYKPSKIISAFSKLMDGTIGIFPDKAISV
                     PYIPDPQSFPAINEQGAECKPYEQTVTEQIINLIGKKYNKQVLVQWNALRSGGKMSKY
                     INMATSQGIAVGFQLAENEFRINASGSDASFDELVKTSIDNKAYYLESWEPALTQCKT
                     ATKNAKSKFRKAAGIN"
     gene            complement(12984..13733)
                     /locus_tag="PK736_02755"
     CDS             complement(12984..13733)
                     /locus_tag="PK736_02755"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRC32344.1"
                     /translation="MALIHSFESSGNWLFRRRSYLPLILFLLAIPAVFTAGYGLMFPL
                     KATIIGWAGILISAIGLAVRSYTIGTTPQGTSGRNTEEQVAESLNSTGIYSIVRHPLY
                     LGNYLMWIGIVIYTLNFSFVIIVSLMYWLYYERIMFAEERFLEKKFGQAYLDWSLKAP
                     AFIPAFKNFIPPVVPFSFISVLRREYSGFFATVLGFVFVDVLRNISINADFEKKYIPD
                     QVIVIVLAGMLTLILRTLKHNTNLLNESGRS"
     gene            13888..14265
                     /locus_tag="PK736_02760"
     CDS             13888..14265
                     /locus_tag="PK736_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010178301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HRC32345.1"
                     /translation="MTKTKYKFEFPMKASPKMLYSYISSASGLGDWFADKVEAVGNVY
                     TFTWDGEKSRAKLSHKREPHLVRFNWLDEPDKTYFEIEIILDEITSDIELVVTDFSTE
                     EEKGELYQLWDSQIHQLKMLIGS"
     gene            complement(14252..14827)
                     /locus_tag="PK736_02765"
     CDS             complement(14252..14827)
                     /locus_tag="PK736_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32346.1"
                     /translation="MELIKSEAFFKTRKKIFDNICIQFQEINNNIEELKSQYDALFKQ
                     YQSPLNGKISKGENYDGCPYMVLDNPRIYLPNNILACRTMFMWGKYFVATFIFKQPDA
                     TKHLNLIVNFVNKHVSENDYLMTGNAMWQHHITDDNYTLLSKMDLKNLEFILPLEHVK
                     IVRVIPFTAHVDLQTQCMSFYKQCLGIITNL"
     gene            14921..16351
                     /locus_tag="PK736_02770"
     CDS             14921..16351
                     /locus_tag="PK736_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRC32347.1"
                     /translation="MKIKKLHLLILKAFLGPFFLTFAISLFVLLMQFLWKYIDDLVGK
                     GLETSVIAELLFYASAQMVPMALPLAILISSIMTYGSLGEHFEIVAAKASGISLLQML
                     QPLLIVALFISAGAFYFSNFILPKANLQAGSLLYDVTHKKPALNIQENVFYNGIDGYS
                     IRVNKKDNDGNTLRGLTIYDHSDNLGNTKVLMADSGKMLFTPDGKAMIVTLHHGSSYE
                     EINNNRSGKYTHPLMRTTFEYEELRFDLSSFAMKRTDKDLFKENKSMLNVYELNHAIV
                     TFEADIRIRQIDYHRSMMMYLAMTKDSINFNKEYARALPQNPYQNITQLQLEQVRTSA
                     LNQANSLRDIFVNLSAEYKMKEENIARYRIEWHRKFTLSIACFILFIIGAPLGAIIRK
                     GGLGVPIIIAIIFFLLFHVTSITGEKMARDMKLSALQGMWLATFVLAPIGIFLIFKAS
                     KDSGILDLDSYKMFFKNLFTRKPKEA"
     gene            16351..17565
                     /locus_tag="PK736_02775"
     CDS             16351..17565
                     /locus_tag="PK736_02775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC32348.1"
                     /translation="MRVLQLCLRIPYPANDGGNIAMFNMAQAMKINNCEVHIAALNTT
                     KHFVNVDELPIDFVNDFHFQSVPIVNKVTAAGAFINLFSKQSYNVSRFISLDFETLLI
                     SILQKNKFDIVVCESLFMAPYVSVIKKYSTAKCILRAHNVETLVWERLAKGEKNIAKK
                     WYINLLAKKLKRYEQSAFNLFDGIIAFTQEDKILLQQMGCKVDIEIIPICIETKKFDV
                     KFDTRQKIKLFHLGGMDWRPNLEAIQWWLQNVYPLCKKEMPQIELHLAGKHMPKDIFD
                     MADNRTLFVYNKIVTVKEFMADKQIMIVPLLSGGGMRVKIIEGLAAGRVVISTSIGAE
                     GIAAIPEKEILIANTPQEFIVQLKSIVDDKLRMQNISKAAKEKADKLYDVNACGAQVK
                     LFLQKIIGNTSK"
     gene            17626..18474
                     /locus_tag="PK736_02780"
     CDS             17626..18474
                     /locus_tag="PK736_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRC32349.1"
                     /translation="MKKNIFPALIFVCISMQAINLTAQSCCSKPQSTQAFAMLTHDDK
                     FVAAHLDPVPFNFTPESGKMVKFETKDGKAANAFLVPAQQESKKYLIVIHEWWGLNDY
                     IKKQCEAYAKDFPNMNILALDLYDGAVAKNKDEASKFMGEAKEERVRAIITGMQAYLG
                     NEAQCMTIGWCFGGGWSMQTAMMLDKQCKGCIIYYGMPEKDKEKLMTINFPILGIFAQ
                     KDQWITPEIYNNFRDAMKINSKQIEIVEFDADHAFANPSNPQFNQEFTTKANDAVYNF
                     IKTFFE"
     gene            18532..18825
                     /locus_tag="PK736_02785"
     CDS             18532..18825
                     /locus_tag="PK736_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32350.1"
                     /translation="MFKKSEVWFGIISGLLMPLVGIFLVRITFFPNQDINKILEFFLM
                     DMDTMSDVLALSLLVNGGIFYLFLKYDKEESARGVVLSTFLWGIVCVCVKFFV"
     gene            19283..20593
                     /locus_tag="PK736_02790"
     CDS             19283..20593
                     /locus_tag="PK736_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC32351.1"
                     /translation="MANYKIKHLLVQNIGVFKHLDLQFPESSVDGNADIHIFIGDNGT
                     GKTTLLEALTYFDKVYNLAFRNKEDKSYSLLIEKAYQNKYYLSIGYEPEIEIIFGDAN
                     IKFKIKSGTSLTSPEDESFFESYFSDFLNPNNYYHTDYSFFAYNGHRQISDTKVESFK
                     ELEFNPIADALSFNKSNLNSNIFQWIANNKTRAALSFQEGKKSDSEKYLRTIQKVEDA
                     IGNIIDRKVEFVIDVHSLKVMINLDNDSINYETLADGYKSVISWLTDLMFRLDYLSTE
                     ENPAFTLFLDEIDVHLHPAAQRRILPVIQKLFPNAQIFLTTHSPFIIGSVDGARVYKF
                     KLDENGHSVLDGEPFETEDGNSYEYILENLFDVHNPFGVEIEKQLKEFYNKRNELIKN
                     TTDLGVDELVASSRALAEQSDELRTLIGIEMRQLSRIIKKDIVL"
     gene            20597..21205
                     /locus_tag="PK736_02795"
     CDS             20597..21205
                     /locus_tag="PK736_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32352.1"
                     /translation="MRKFKRAESPDFLTKKWHEWGNSYKENKTKNSGFTFQWPNHESI
                     KINVILKPLLAQLTGNHCSYCDDYPLKTKADSIDHFKPKSRSEFFELVCQWENLFYCC
                     TNCQNSKKEKFHVHLLRADRVDFSFSKYFIYDYTNHKIEPNPFAEKIEQESATISIEI
                     FGLNDPGHVSSRRISLNRYNSQPKDKSIFIDDFPYRYILENI"
     gene            21209..22312
                     /gene="lpxB"
                     /locus_tag="PK736_02800"
     CDS             21209..22312
                     /gene="lpxB"
                     /locus_tag="PK736_02800"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRC32353.1"
                     /translation="MKYYIIAGEASGDLHGANLLKHLKQKDAHANYRAWGGDMMQEQG
                     AEIVKHYRDLAFMGFAEVIANLRTILRNIEFCKNDILQYKPDCLILIDYPGFNLRIAE
                     FAHQQNLKVVYYISPQVWAWKKSRVYKIKKFVDKMLVILPFEKDFYKKYDYETTFVGH
                     PLLDAIENYRNKRLSPSQIKQPYIALLPGSRMQEIKVMLPLMLEAAMQIKNYEIAIAG
                     APSIDATFYESMISNSGAKIIYNNTYHLLQYAHAAFVTSGTATLETALFRVPQVVCYK
                     GNWISVQIARRLIDIRFISLVNLIADKAIVKELIQQELTTQNMVSEMQKLLQGEKRNN
                     MLREYDKLIALLGNAGASEKAAQEIFDFLNGMD"
     gene            complement(22371..25730)
                     /gene="mfd"
                     /locus_tag="PK736_02805"
     CDS             complement(22371..25730)
                     /gene="mfd"
                     /locus_tag="PK736_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRC32354.1"
                     /translation="MNLDQLIKKYSAHPLFGELVKNHNTTEVQHVHLDGLRGSSIALL
                     IAALYKHEKFNAVFVLNDREEAAYFANDLENLLGTTHVMFFPASYKKTGDPTSLDNSN
                     VLQRAEVLNRISKNPAGNLIITHTESFYEKVTTKHSLHQNTFDVKRGSVMSQDFLSEF
                     LDDNDFVQTDFVYEAGQYAMRGGIMDVYSFAFELPFRIEFFDDRIESIRTFDPSNQLS
                     VQQMDVISIIPNIQTKLLKQTKEHFLNFIGNNYRIWMRDYDSACESISALHNKMSELI
                     ITVEEADTPPIENFSGVLEMQELMQQFKIVEFGNRASFDDAAKLHFNVQAQPSFNKNF
                     NLLFQTIHENQTQHIESLIIADQPKQIERIYAIFDDIGKTFLAKGQNINFTTLLLSLH
                     EGFVDNDLKLACYTDHQIFDRYHRFRMKKNFAKGDSISLKDLYALRPGDYITHIDHGV
                     GRFGGLETIEANGKTQEAIRLLYKDNDILYISIHSLHRIARYSGKDGTAPSLNKLGTS
                     SWSVLKQKTKKKVKDIAKELIALYAKRKIQKGFAFTPDTYLQTELEASFIYEDTPDQV
                     KSTADVKADMEKPHPMDRLICGDVGFGKTEIAIRAAFKAVCDGKQVGVLVPTTILALQ
                     HYRTFSERLKDFPVTIDYINRFKSSAQQKETLIKLKEKKIDILIGTHRLLSKDVKFDD
                     IGLLVIDEEQKFGVGAKEKLKELRVSVDTLTLTATPIPRTLQFSLMGARDLSIINTPP
                     PNRFPVTTELHMHTDKVIKDAVMFEVSRGGQVFVVHNKISDIHDLAEKIKRLCPNVRV
                     AVGHGQMEGDLLEEVLLGFIEGDTDVLVSTTIIESGLDISNANTMIINQAQNFGLSDL
                     HQMRGRVGRSNKKAFCYLLTPPLTTLTGEARQRLKAIEEFSDLGSGFNVAMRDLDIRG
                     AGNMLGGEQSGFIADIGFETFHKILDEAIFELKHTDFKDEFKEEYAKEEFVRDCQIDT
                     DLEILLPQDYVTSITERLNLYKELDDITEEQKLEEFATMLHDRFGPVPRPAIELMDTI
                     RLRWKAKALGFEKIFLKNNLFKGYFVNDDKFFQSPLFSGMIGFVNSHAHQCKLKDDKG
                     KPSLVVNNIGSIDSANKILQKMLNTSNALAN"
     gene            26048..26485
                     /locus_tag="PK736_02810"
     CDS             26048..26485
                     /locus_tag="PK736_02810"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRC32355.1"
                     /translation="MKKAIGLLFVASTLFFSCKKESQQEIKNIEINVTMKMNSTYSNN
                     VIHPNDQDEDDIVHITKDTQHNAKTTLVINADNKSSLFTYTPQSDQIGTDQVIITGGD
                     AEEDHQGHCGNNSGGKGHHAKGKMGGHHEEVVTNYIFNITIVQ"
BASE COUNT         8161 a         4959 c         5064 g         8519 t
ORIGIN      
        1 gatttggata caaagtgcct tttgttttta tagtatttag cattgcatca tttattacat
       61 ttttgccttt gcgttgtggt ggcattacat caataatatt caaatgcaaa atggctcgcg
      121 cccaatacac ggcttcgcct ccatagaatg ggtgcatcaa tgcaatattt aaaatcgagg
      181 caatggtatc actatcggca tctaaacttg catcgtaatt taatgctacc aatgtggcta
      241 tgtatttttt gttgtattca attacatttt ggtcaactat taaattgagt ttattaagcg
      301 caaaactatt ttttccatta ttcaaagaat caacaacttc tttcatcgta cccatatttg
      361 tttattttaa actgtcatat tcggcaatat attttgcatc gttatttgtg cccaatgcaa
      421 gtatggctgg atttgtagta gcacttttaa aaaatttttc tttatcaatt attacaaatt
      481 ctaacgaatc gtacccatct gttgctgtac tatcaccaac agcacttcca aatatccgcg
      541 cacgctcgcc atcgtttatt ggaggaggtg tatagcaatt gtgcaagtca cctggttgcc
      601 ttgttacaaa tcctgcactt gggtcagggt catattcgct gcttaatgca gggtcgtata
      661 tccatgtgat attttgctgt aaccaagttg caccatcaat tttaaagcca cctatgttat
      721 ttatccattt attatcccag gtggtgctgg ttgcttgtgt tgcaccaaat attccctgag
      781 tgctgagaaa acttgtagga cttgcatcat caagatatac gccattccaa caattttcca
      841 tttcgttgca tagcaaactg ctattactac aatcgtcaaa cacatcaata cctgcaccca
      901 tgttggtcat tgtgttttct tgcaagcggc agctgaggct gctttgtagg cgtatgcctt
      961 gtatcatatc tctttcttct tgaacggtaa tagcgcttgt ccatgtagca tggttttgga
     1021 atatggtggc gccattacag ttttcggccc atacacctat atgagggctt acactcccag
     1081 cattgtggtt attataatag aagtcaataa aaaatgccga tagttcggta gtgtattgat
     1141 tattattaac ggtggtgttc acattgcgta tgtgtatacc tatggcaaca ttaaatattt
     1201 cgttgtcggt tatcgtatat ggagtataag tgtcgaacaa aacatattcg caacgtatgg
     1261 cctcatcatc ataaaagtct tcttctaatt gaaaatattt gttaaaagaa tttgcatgca
     1321 cagtggctac tgctgttaca ttgttaaaca tttgtacccc ttgtttgcat ctatttatgg
     1381 cattgttttg tataattata tttttacaag cactttcttg tacaccttca ttgctgcgat
     1441 caaactgccc catattaatt atattatttt ctatattaag gtttatacag ccgcgagcga
     1501 aaatattttt ttgccaattg ataaatgtgt tgccgttgct taacccacct atggtatggg
     1561 tatgtggcga taaattaata tttgaatatg aaccatttaa acttattgcc tgagcatcat
     1621 gagtactatt aaaattatag agtttttgcc aatcgaataa attattctga attataatat
     1681 tgctttggtt tgtcaatacc gcagttacag cattacgaaa tatattttgt ggtaaaatat
     1741 taacgccaag tgtaaatcgt gctacattat ttagcacgac atggttatca gtaagttctg
     1801 ctacatgggg agcttttgta atttgcccat cgttgcaatc gaaagtgcag cctctaatgt
     1861 atgagtaaaa ataatcgcca ttgttaagcg aaactccacc aaagttgtgg tcgaatgtaa
     1921 catttgataa ttgcacttta ctgccattgc catcagctct tgttgcaagt attgcatcct
     1981 ctattgtgct gttttctatt ataaccgtac caccattatt tattattcct tgccacatat
     2041 cgccacaggc ataaatgtaa caattggtca atttcagtac atcgccctga ccacaaatta
     2101 tacttacgcc ttggcttaca ctaagtgtgc aattttccaa tatgagttgt ccagccaaat
     2161 tcaaatcatt cgtgagcctg agaaattcat tttgtacagt tccacttgtt gtagtaaaag
     2221 gagtaacttg ccatcgccat tctcccatac cacgcacaca agcatataaa aattcgttat
     2281 cacaatcgct accattggtg cccatgcact gtacagtttt gtttggcata ttgcctgtta
     2341 tatcagtcca acacggctct attgtacctg tatttatatc atatataccc ttataaactc
     2401 ttgctcgtgg acctgttcct tcattttgcg tggcagcaac atatatggtg cgaatatttt
     2461 cggcaacagc cagggcggtt attccacggt cgttttggtc gccatcaaac agatcaataa
     2521 atatatcatc ggcaggcaca ttgccattac aatcattatt aatttcgata ggaactaatg
     2581 cgcccacatc gctattgtat acttctacga gttttgaatg cgtgttggca ttccgtggag
     2641 agcaagccag aagcactttg tatcctaagt cataattgta tgtgatcaac tcctctgcta
     2701 ttacatcagc gtttgcggta ttgccattta aatccaatgg tgcaccaact ggactccacg
     2761 cggttgccga agattttgta agttgtatca cattgtcatt gcttgcaaag ggtattgcaa
     2821 acatggtatt tccatcaggg ctcaatacca catcgcgaat gcttgtaatg gagccaactg
     2881 tacaaaggtt gagcgtccct cctagggtaa aatggaataa ctcacgcgga cctgcaattg
     2941 ttgttaccaa agcatacata tcattccctg acgagaaaag tatttttata gggtctccac
     3001 atacagtatt tgtgactgca tttgtccaac tggtggtgcc agcaggcaac ataataatgg
     3061 tgccccgccc catttgttca ccagggccct cggccgcacc ggcatacaag ttcaaatcat
     3121 tacttaaagg gaaagtatta tttgtaattg aaaaacaatc tgtcaaagct aaatttgctg
     3181 aggtatttcc gcaaccggat actgccggta taaaagcaat atttctacaa agtgtggtat
     3241 tggttacatc aagcattgcc gtaaatcctg gtaagtttga aaatgacatt aacctgtcac
     3301 gttcagagta atagagttcg gtaccaatat gaaaaatact ttgcgatgca gtatttacaa
     3361 atccacgatt gccatacgct acatcagtac cacaagcatt ggtaacaatt tctgtatata
     3421 tttgttgcca attgaccgct gcgccttgca catcagcttt cgaagcgaaa atgttaccat
     3481 tttttccaac aattaaataa tttcgattta aagtaccttg taccatgcag ctgctattaa
     3541 tatttgcatg agggcgtgta gtattccagc caaactcatt agctgggtcg cttgcatcca
     3601 ctgtgagcgc tgtgaaaatg cccgtgggcg ggccattgaa tgaagcggca ggcgagtaca
     3661 gtatgcctac atagacattg gcaggtgagc cataggccac agtgggcctt tcgttcatta
     3721 gtaaataacc ggtaatgtcg tttgtgttat tacgtataaa atgtaattgc gtgtaatttg
     3781 gcaccgtaga atttgtggtt acatcgtata taatgttgtt gctattgttt ctggcaaata
     3841 ttgatgtaac taaatttgct cctgttgccc aggtatttga actactatta tagtcataaa
     3901 tgccttgtcc atgtataaca acataatagt gatttgaact gtattgctga atagaggtaa
     3961 catgataggg aattccacta ttattagggt tgggcactaa attattaagg gcagtaattg
     4021 tactagatgt aacatcgtta taagtaaaat gatataaacc gacatctgta cctacaagaa
     4081 atttgtctaa atttctgtta ttattaattt caattacttg catacaataa acatcacgtt
     4141 ggattcccat ttgtggtaaa ttaataccga taaggttgtt tatggcgtct tcattcgctg
     4201 tcaaatcacc ataataaaaa aacagacata gtgaaacttg taataaatga tatgtttggc
     4261 tgcattatta attcacaaga gatgttgaat gaaaggcaaa aattaaatgt aacaaattat
     4321 gcccaatcaa tttattatat gcatgtttat gtaaatggaa ttgattgtga agtacatcgt
     4381 tttactgtta taaaataaat ttttaataaa aggagaagag ccattttgct ctcctttttt
     4441 atttataaaa gcaatgaaaa aatttatttt aataatccat ttttgttttt ttttagtact
     4501 gtttactctc aaaatcaaaa taatgcatgg gtatttggtg atagttgtgg tatagatttt
     4561 acaaataccc aaaacccaac accgataata actaatatgg ttggtcgcgg aagctgcgtt
     4621 tcaatatgtg atacaaatgg aattctaatg ttatacgcat atacggttga gagcagtggt
     4681 gcattatcca ctattgtgcg caatggattg catcaaaaac taaatcaaaa taatactata
     4741 acaggtgtcg cgtggtacaa cgaattagct ttgtttccta aacccggcac acaacaaaat
     4801 ttgtattacc ttttttcggt tggactaaat ggtgcgccaa atccgggctt gtatcatact
     4861 acaattgata tgaatttaaa caatggctta ggtggcattg tacaacaaaa tatacaaata
     4921 gataatattg agtatggcga ttgcctcact tgtgtaaggc atggcaatgg tcgcgattgg
     4981 tgggtgatgg caaaaataaa taattttcct tttacatttt acaatcgctt tcgtactcgt
     5041 ttagttaccg cagatagtat ttatcctgca atgattcaag actttaatga tgcctcggat
     5101 ggctcgtttg aaaaaatttg ttggcatcca agttataata agtttatgat gatagatgtt
     5161 aggggttata tgtcagaatt tgattttgat aggtgtactg gcattatcac tcaagtcaga
     5221 acaatcttcg ctgaacagct gagtaatttt agtaatttat tttgggaagg agcatattct
     5281 ccaaatggca atgtttttta tgtgagtaga acttcttatg gaggtaactt tggtgattat
     5341 aattatttgc tccaatatga tttaaccgca atagatatac ctgccagttg cgatacactc
     5401 gatagcacca tttacccgca agcagattgc ggagctgtgc ggttggctcc agatggtaaa
     5461 atatattact cgcaagccta cccttggggt tttccatacc aagatagtat gcacaatgtt
     5521 gtaaacgaga atttgggtgt agtaaatttt ccagatagtg taggcaaggc atgcaatttt
     5581 ctgcccttct ctttttacct tggtggtaag cgtacttatt atgggttgcc taacaatccg
     5641 gattatagtt tggggccgct gcaaggttcg ccatgcgata ctttgcaatg ggttggcaca
     5701 cccgaaaccc ccaacctcct gcaagggggc tttaggagct tgcgcatcaa tcctaatcca
     5761 gcaaataatt ctttttatat aaagtatgat attcctacaa atgagaattt actttttgtg
     5821 ttgtatgata gttatggcaa agaagtattg cacaaaaatt tgtatggcac ttttaaaaat
     5881 ttgttggtgc atacagagca attaaacaat ggaatttatt tttggcgggc ggtgaaagac
     5941 tataatacga aaacccagcg cagtaatgga aagtcccctg aaggggattt aggggttggc
     6001 gctcaaaatg gaaaaattgt tattctacat tagagttttc ttcttcaaaa tcaaaattca
     6061 gacaattgca catcacaaag cagttggaga aaactccaac tgaggcaaga aaatcataca
     6121 ctggttatca tcaatcggag attgacatac actgtgacgt agtcggagac tacgccaagc
     6181 taagagtaaa ctccagctga ggcagcaaat attaaatttt cacaaactta tcagtaacaa
     6241 caaattcgtt gctaatcaat tttaagatgt atgctcctgt atgcaagctt tcgatattga
     6301 tttcattttg gaaagtatca tcaatatttt ttgtgagcat aagtttgcct tgtaagtcga
     6361 atatctgaat tgtgccttgt gtgatggtag ttggcaatgc aacattaata aacgattgtg
     6421 ccgggtttgg atatacgctc aatgtgcttc ccatgttttc cactgattca aatgccaggc
     6481 gggccggagt aaattgtcta cggccatact ttacaaaaaa ctcgtttggt aagagcgaca
     6541 ggtcatcaac atccatcgaa aaatatacct gattgctatt gcgtaccgat atgcgttttg
     6601 gtgtatatgt taaatctaag cggaaaggag tttgataatt ttcctgatag ataacgcttg
     6661 taactccggc cgtgctgatt cccgaaacct gcactccgcg gtattcaaaa ggattgccat
     6721 tgaaattttg agtgtaagaa attctgccaa aaacgtaaag gttattatct gcccccttcg
     6781 ctacatcggc cactttaaaa ttaagtgatg ttaatatcgt ggtgccaagt gttacaattc
     6841 cggtagcgtt acttattctc agatatttat tcttgttggt gtacatgatt aagccacctg
     6901 tactttcagc aaaaaaatcg aaacattcat tgtcatcgta actgtaccgt tttaaatact
     6961 gcaatacacc cgatgaatta tattttaata gaaaaggtct tggctttggt gatgctgcat
     7021 tggaaaaaat atgacctgct atgaaacaat ttcccgaagc atctaactgt aaagttttca
     7081 caacatcgcg ctgaacaata gcaccaaaga tgctcgcctt ttttatgata gtgaatacaa
     7141 agttcaaatt gttatccatt ttaagtaaaa ctgttgaacc ttgaaacggc cccatatcag
     7201 ttgtgcctgc aacataagtt gcattattca aataacgaat atcgacaccg gtgttgctat
     7261 catctaaatt cgaattttta aaatttcttt tctttagtag attaccagaa gcagaatcta
     7321 actttagtac acaaatagaa tcggcactat tgccaagcac taataaactg cccgcattgc
     7381 cttttattat ttttacaccc ttatcttcga gatctcctat gccattgtaa atagaggacc
     7441 atttcaaatt caagttcaag tcatatttgt aaattaaaat atcgtgatcg cccggaggaa
     7501 aattatattg cccgtccatt accacatata gtgaattgtt gtaaacctta atgtctttta
     7561 tcacatcatc gaaagggctt gtactgtaac ccagataaat ttgtttttga aatactcctg
     7621 ccgatgaaat tttacgtatg cagggttggc catttgcggc tgtagaaata cagcgcgatt
     7681 ggcctgcaag gaaaagattg cccgatgcat caatggtgga caggttgcca actgtaaaaa
     7741 ccgtatcggc agtgctgctt ttataagaat tgccattcca gtcttgtgcc atcaacatgt
     7801 tcatagacag aattgaaatt aataagcagg ctaaaatttt aatttttttt gaagtttttg
     7861 taagattcat aatggtattt ttttttggtt atatcgcaaa tgtaatatct tttgaagggt
     7921 ttggctttta tttttttgaa cagaagtaaa ttaatttttt gagcgtgata gagtttggtg
     7981 aggatgccag catgggaatg agtgttgacg tttggcgggt acaccaaccg aggacaaatg
     8041 gcggttggcg aggacgccaa acgaggacag agaatacaat tggagaaaac tttgatttag
     8101 gcagcacaga tactattgga agtgcgaaga gaatgataca atgcgcgacc gcgcagtgta
     8161 acggagaggc caccgcctcc cgtaataaag aaatatttta ttgctgcacc atcataaact
     8221 ttccggggcg taaggggtcg gctacaaacc atgcgggatt gccttgcggg tctttaccta
     8281 aacgtgcatt tggaacagga ggcttgctta catcaacatt ttttgtgaca gctaccggtt
     8341 cggttttggc ttggccgttt gtagtttttt tatgtggaat aaggtcgagg gcaatgagtt
     8401 cgtttaccga ttttttgaat gcgtcaacta taaattgcac atcggcttcg gtgtgcgatg
     8461 ttgtgaggaa acaaggaaag ccatcgagca catgcacgcc attcattcgc cacaggtgga
     8521 ataaaaattc gctgaatgga tactcgtgtt tgtatttcat tttgaagagc gaagcaaagt
     8581 tggtgaaggc aattggcaaa tcgagcgcgt agcaaaatgc attgagttgc tctaccatac
     8641 cttttgtttt ggcattgagt tgttgttgca gttgcgggcc ttgggtttta agatgttgca
     8701 acgatgctac tcctgcagca agcgcaaatg gatgccgaac aaatgtgccg gcaaaatatg
     8761 ttacacctgc ttcgggaacc gatgcatcgc catattgcca catgcctcca tcgagggcat
     8821 ccatatattt tttggtaccg gccatagcgc caataggcat accaccgcca cacacttttc
     8881 cataagttgc tatgtcggct tttacaccat aaaactcttg tgctccccct tgtgcatagc
     8941 ggaatcctgt aatgacttca tcgaaaatga gcgccacatc cgcttcctga gtcattgcac
     9001 gcaattgcgt aataaattct ttaggctgaa aatcggccat gcggctttgc acaggttcta
     9061 ccaatactgc agccagttcg tgtaaatgtt gctttattat ttgcaatgtt tcatcggtgc
     9121 cataatcgag tatgagcata ttttcgacag caccgggcat aatacctgcc gatgcgggca
     9181 ttgattttaa tttctttgta ccgcgcacaa ttacttcgtc attgataccg tggtaagaat
     9241 tgttgaaagc tacaaccaaa tttttgccgg ttacagtgcg tgccatacgc aatgcgccca
     9301 atacggcttc gctgccggtg ttgcacaatg ctgcgcggtc gctgcctgta agttcacaaa
     9361 tgagttgtgt aagttcgcca gcaaggggat gttgcggacc aacttcgtat ccttgttcca
     9421 attgttttgt aagtgcatca actacaaatt gtggcgagtg tccaaacatg ttgctgccaa
     9481 atccattgag tacatccacg tattcgttgc catcgatatc ccacacgcgc ggacctttcg
     9541 atttatttac tactacctga taagtaattt ctttgttgta aggcttaaag cctgttacta
     9601 cacgcggatc ggcaaggtga gcgcggtgta actgtgtgaa cgttttcgat ttctgcgtaa
     9661 gtgctacata gcgttttaca aaatcgttaa accacttttg ttgctcgggg ttgagggtgg
     9721 tgcttacatt tttttcgata cgggcaatgg caccaaacgg ttttgcttct tcttttggct
     9781 cttcggtttt tgtttcggtt gctgtttttg cttctgttgc tggtgtttca acgattggtg
     9841 atgcaacggg tgcagaaggt gcagccatac tttgttgtgg ggcttgcaca ggcattcccg
     9901 aaagcaaagc cagttgctgt tgcattattt gcatttgctg ggtaataagc cattcaacac
     9961 taccggcatc ggcattaact gtaggtgtat acgtagcttg aggttgcacc ggcatttgca
    10021 tctgttgtgg tgcaggtaag gcaggtgtcg gttctatatt cgttactgct gcaggttcac
    10081 tgtatgtaac gctggcaggc aaatgattat cgataaaggt ggccagtgct gaaatagaag
    10141 atagttcttc gttcaattgg cggaaagtaa ttttggtttg atattttttt gatagtgtaa
    10201 ttgaaacctg cgtaagaaaa agagaatcga ggccaagttc tattagtgaa gaatttaaat
    10261 cggccgatgc catttctata cccgatgctt cttctaaaat ttctttaagt tctgttacta
    10321 aatgttgctg acgacccatt atggtagtgt tattaattaa tgtgttattt ttgttttcta
    10381 tcgaatggtc gataacggtt tggttatcgc tttcaaaagt ttgattatct gttgctccaa
    10441 aattttcgag tgcaaatgta tttatttcaa aactaagatt gtcttctttt gccgattccg
    10501 aaataatatt ttcttccatt acattgtttt caatttccgc agtaatgttt tctataaaag
    10561 gcaattcgag gtttattact tcacctttat taattgatga agtattctga tctgtcatat
    10621 tgtcgagcca gagagcgtta tcttccaatt cattttcaac aaaagatatt tcctcgtcca
    10681 ttttcattaa atcatcgatg tcggaatttt gctggttttc tatttcatcg gcatcgtctt
    10741 caacatctaa atcaacatcg tcatcttcat taccaaagtt caaatccata ccttgtatgt
    10801 gttcaataat ttgttgatcc caaaaaggca tatcgagttt attgagttca actttattgt
    10861 tattgataat gctgtgccac aaacgataca aaagcgaatc ggaatattct acaattttat
    10921 cgtcaacaaa aagttcggag ctttcgaagg ttgtatactt tgtagaagca ccattttcgc
    10981 cattaaattc ttcaacaaaa aaatgtcccc ctccaaacat ctcttcacta tctgttacgg
    11041 cattggcatc gttgctggtg tttgggtcta ccaatataat cttgggaaat aaatctgctg
    11101 cattgggtag cgtggtgata aggcaattga aggcagcgct atctgcttgc tgttgcaaaa
    11161 aatcgggctt taggttatcg cgaaacaaca tttggttggc gccaagtttc gacaatgcca
    11221 tagttgcggc acacaatgca aagccgggag gaagatatag cgccacaaaa tcgccttcgt
    11281 ttataccttt gttatataat tcggtggcaa gggcatcgct ccaaaagtcg agcgcctgca
    11341 atggcaattt tgtatcgttc atatgcacca tcacatcggc acctgctgta tcgaggcgtt
    11401 gctttattac ttcgaataca ttgtgcgatg atggctcatc atgcatggtt tggttactta
    11461 tcattccctt caaatttagt gatggtaaag gtgatgataa gcagtttatt gaaaggctat
    11521 ggtaataact ttaaaggtct ttttgttaac acaagaaagt taattgcgca gagttaagtc
    11581 tgcaattatt aaaaatactg atgaacaaaa ttaattaatg ccggcagcct tcctgaattt
    11641 gcttttggca ttcttagtcg cagttttgca ctgagtaagg gcgggttccc acgattcgag
    11701 atagtaagct ttgttatcaa tagatgtttt tactagttca tcgaacgaag catcacttcc
    11761 cgaagcattg atgcgaaatt cattttctgc aagctgaaaa ccaacagcaa taccctgact
    11821 tgtggccatg tttatgtact tcgacatttt acctccactg cgcaaagcat tccattgtac
    11881 caacacttgc ttgttatatt tttttcctat taaatttatt atttgttctg ttacagtttg
    11941 ttcatatggc ttacattctg caccctgttc attaatggca ggaaaactct gtgggtcggg
    12001 aatgtaagga acactgatgg ctttatccgg aaaaatccca atagttccat ccatcaattt
    12061 agaaaatgca cttattattt tcgatggttt ataaccggca ctttgccaaa tcacaattgc
    12121 atctgaagta acatccttac cattggttac tttatcctgc gatggcaacc gcatttcgtt
    12181 tgtgttgata ttcatgccgg tgaattttac cattatcaaa gcatcataaa aaccgggttt
    12241 ggcttttaaa tgatcgcgca gtgaggtttg catatccaaa ataaaggatt ggtacttact
    12301 atcccagggt ggtgccactg ctaccgagaa agcttttttt cctttgccac gatgattaat
    12361 ttcgctgaat tgcaattttg gtactcctgc accaaacacc cattcgggag tataccttcc
    12421 cgatacaatt cctattacaa cttttttgtt atattgaatt gcctgagcaa tttgttgatc
    12481 aataaaactc cagttgtatt tatcgtcttc gggttctata ctgttccaac tcaaccgaag
    12541 gtaaatacct tcaatatcct cgttgctaac tacgcttggc gaaaaacccg catcaattgc
    12601 cgaacttgta atatatatgc cggtgggcat ttgtatccca tttgttggtt tggtcggact
    12661 taaaggggta tcgttgtttt ttggagtgat attttcatca ctattattca cggttgattt
    12721 ttcaattccg tttacattct tatttttttc attcgattca cttacattct ttttcgaact
    12781 acaagcaact atgctcaata aaaatattaa aatatttgtc gataaaatct tattcatttt
    12841 tctatttgat ttttaaagtt gcaaaaatat cactttaaaa aaatacttat cgccaaattg
    12901 atgaagcgac cagaattatt ttacacacta caaaaagctg tatcaatttg cattcttaat
    12961 ttttaatctt caatctataa ttcctaagac cttccgcttt cgttcagcaa attagtatta
    13021 tgcttcaagg tgcgtaatat taatgtaagc attcctgcca gcactatcac tattacctgg
    13081 tcgggaatat attttttttc aaaatcagcg ttgattgaaa tattgcgcag cacatccaca
    13141 aaaacaaaac ccaacacagt tgcaaaaaaa cccgagtact ctctgcgcaa aacactaatg
    13201 aaagaaaatg gaacaacagg aggtataaag tttttaaaag ccggaataaa tgccggagct
    13261 ttcaatgacc aatcaagata agcctgtccg aatttttttt ccagaaaacg ttcttcggca
    13321 aacatgatgc gctcgtagta taaccaatac ataagactaa caataattac aaaactaaag
    13381 ttgagggtat atattacaat acctatccac attaaataat tgcccaggta taacggatgg
    13441 cgcacaatag aataaatgcc ggtgctgttg agcgattctg caacttgctc ttcggtgttg
    13501 cggcccgatg tgccctgagg tgtggtgcca attgtatagc tgcgcactgc aaggccaatg
    13561 gcacttatca atattcctgc ccagccaatg attgttgctt taagcggaaa catcaaacca
    13621 taacccgcag tgaatactgc cggtattgcc aataaaaaaa gtatcaacgg caaataactt
    13681 ctacgcctga aaagccaatt gcccgatgat tcgaaagaat gaattaacgc cataaattgt
    13741 ttaaataatt aacagagatt tttgaggtaa cgaaaactta gcaaattaat gaaataaaaa
    13801 cttcattttt actttgcata ttaaatcatt tttgttgacg tttagctcgg attttaaaat
    13861 actctctcaa cattaaatat tttctttatg actaagacaa aatataaatt cgaatttcct
    13921 atgaaagcat cgccaaaaat gctctacagt tacataagtt ctgcatcggg attgggcgat
    13981 tggtttgccg acaaagtaga agctgtgggc aatgtgtaca cttttacatg ggatggcgaa
    14041 aaatcgcgtg ctaaattaag tcataagcgc gaaccacatt tagttagatt caactggctt
    14101 gacgaacccg ataaaactta tttcgaaata gaaattattc ttgacgaaat taccagcgat
    14161 atcgaattgg tagtgactga tttttcgacc gaggaagaaa aaggtgaatt gtatcaatta
    14221 tgggatagtc aaattcatca gttaaaaatg ctcataggtt cgtaatgatg cctaagcatt
    14281 gtttgtaaaa agacatacac tgcgtttgca aatcaacatg tgcagtaaaa ggaattacgc
    14341 gcacaatttt tacatgctcc aaaggcaata taaattccag gtttttcaaa tccattttac
    14401 ttaacaatgt ataattgtca tctgtaatat gatgttgcca catagcatta cctgtcatca
    14461 aataatcgtt ttcactcaca tgtttgttta caaaatttac aattaaattt aaatgcttag
    14521 ttgcatctgg ctgtttgaaa ataaaagtgg caacaaaata tttcccccac ataaacatgg
    14581 tgcggcaggc gagaatatta ttgggcaaat aaatacgggg attatcgagc accatgtaag
    14641 ggcagccatc ataattctca cctttgctta tcttaccgtt taatggcgat tgatactgtt
    14701 tgaatagggc atcgtactgc gacttcaatt cttctatgtt attgtttatt tcctgaaatt
    14761 gaatacaaat gttatcgaaa attttttttc tggttttgaa aaaagcttcg ctctttatta
    14821 attccaattc atttggttca aaaaataatg gctccatcat gccacaatat taatgcattt
    14881 ttacttttgc acacgtttat aaaattaata gcaccaatag ttgaaaataa agaagcttca
    14941 tttacttata ttaaaggcat tcttagggcc attttttctc acatttgcca tcagcttatt
    15001 tgtactgctc atgcaatttt tgtggaagta cattgacgac ctggtgggca aaggcctcga
    15061 aactagcgtt attgccgaat tgctattcta tgcttcagca caaatggtac caatggcatt
    15121 gccgctggcc attttaattt cttctattat gacttatggt tcacttggcg aacatttcga
    15181 aattgtggcc gccaaagctt cgggaatatc gttgttgcaa atgctgcagc ctttacttat
    15241 agtggcatta tttatttcgg caggggcatt ttactttagc aattttattt tgcccaaggc
    15301 caatctgcaa gcaggttcat tgttgtatga tgttacgcat aagaaacctg cacttaatat
    15361 acaggaaaat gttttttaca atggcataga tggttacagc attcgcgtaa ataaaaaaga
    15421 taatgatggc aatacattgc gcggcttaac catttacgat cattccgata atttgggcaa
    15481 tacaaaagtg ttgatggccg attcaggcaa aatgcttttt actcccgatg gcaaagccat
    15541 gatagtaaca ctgcatcacg gcagcagcta cgaagaaata aacaacaacc gcagcggaaa
    15601 atacacgcat cctctcatgc gcacaacttt cgaatacgaa gaactccgtt tcgatttatc
    15661 gtcatttgcc atgaaacgca ccgataaaga tttgtttaaa gaaaataaat ctatgctcaa
    15721 tgtgtatgaa cttaatcatg ccatagttac gtttgaggcg gatatacgta tacgccaaat
    15781 agattaccac cgctctatga tgatgtacct tgccatgaca aaagattcga taaatttcaa
    15841 taaagaatat gcacgtgcct tgccacaaaa cccttatcaa aatattacac agcttcaact
    15901 cgaacaagtt cgcacatcgg cattgaatca ggcgaattcg ttgcgcgata tttttgtaaa
    15961 cctaagtgcc gagtataaaa tgaaagaaga aaatattgcc cggtatcgaa tcgaatggca
    16021 tcgcaagttt actttatcca tagcatgttt tattctgttt attataggtg cacctctggg
    16081 cgctatcatt cgcaagggtg gtttaggcgt gccaatcatt attgcaataa ttttcttcct
    16141 gctgtttcat gtaacttcta ttaccggtga aaaaatggcc cgcgatatga aactttctgc
    16201 cttgcaggga atgtggctgg caacttttgt gcttgcacca attggcatct ttttaatttt
    16261 taaagcatcg aaagactcag gtattctcga cttagattca tacaaaatgt ttttcaaaaa
    16321 cctatttacc cgcaaaccca aagaagcgta atgagagttt tgcaattgtg tttacgtatt
    16381 ccttatcctg ccaatgatgg tggtaatatt gctatgttca atatggcaca agccatgaaa
    16441 ataaataatt gcgaagtaca tatagcagct ctcaacacta ctaagcattt tgtaaatgtg
    16501 gatgagctgc ctattgattt tgtaaatgat tttcattttc aaagtgtgcc gatagtaaat
    16561 aaagtaacag ccgctggcgc attcatcaat ttgttttcga aacaatcata caacgtatcg
    16621 cgatttataa gcctcgattt cgaaacatta ctgatttcaa ttttgcaaaa aaataaattt
    16681 gatatagtag tttgcgaaag tttatttatg gccccttatg tcagtgttat aaaaaagtat
    16741 agcacagcaa aatgcatact gcgtgcacac aatgttgaaa cccttgtgtg ggagcggctg
    16801 gcaaaaggag aaaaaaatat tgccaaaaaa tggtatataa atttgctggc aaaaaaattg
    16861 aaacgttacg aacaaagtgc attcaatctt ttcgatggca taatagcttt tacgcaggaa
    16921 gataaaatac tgctgcagca aatgggatgc aaagttgata ttgaaattat tcctatttgc
    16981 attgaaacaa aaaagtttga tgtaaaattt gatacccgcc aaaagataaa actttttcat
    17041 cttggtggta tggattggcg accaaatttg gaagcaattc aatggtggtt gcaaaacgta
    17101 tatccattgt gcaaaaaaga aatgccacaa atagaattgc acctggccgg taaacatatg
    17161 ccaaaagaca ttttcgatat ggccgataat cgaacacttt ttgtttacaa taaaatagtt
    17221 accgtaaaag aatttatggc cgacaaacaa ataatgatag tgccattgtt atcgggtgga
    17281 ggtatgcgtg taaaaattat agaagggttg gcagcaggcc gggtggtaat ttctacaagc
    17341 attggtgccg aagggattgc ggctattcct gaaaaagaaa ttttaattgc caatactcca
    17401 caagagttta ttgtgcaatt gaaaagtata gttgacgata aattgcgcat gcaaaatatt
    17461 tcgaaggctg ccaaagaaaa agctgataaa ctgtatgatg tgaatgcctg tggtgcacag
    17521 gttaaattat tcttacaaaa aataattggc aacacctcta aataaattaa cttctgaatc
    17581 gttgaaaaaa taattttact tttgaaccaa ataaaaaaaa taatgatgaa aaagaatatc
    17641 tttcctgcct tgatatttgt atgcatttcg atgcaggcaa ttaatttaac agcacaatca
    17701 tgctgtagta aaccacaatc gacacaggca tttgccatgc ttacacacga tgacaaattt
    17761 gtggctgcac atttagatcc tgtgcctttt aattttactc ccgaaagtgg taaaatggta
    17821 aagtttgaaa caaaagatgg caaggcggcc aatgcatttt tggttcctgc acaacaagag
    17881 tcgaaaaagt atttgatagt tatacatgag tggtggggct tgaacgatta tataaaaaaa
    17941 caatgcgaag catatgcaaa agattttcca aatatgaata ttcttgctct tgatttgtac
    18001 gatggtgctg ttgcaaaaaa caaagacgaa gcatcaaaat ttatgggcga ggctaaggaa
    18061 gaacgtgttc gcgctataat taccggcatg caagcgtacc ttggcaacga agcgcaatgc
    18121 atgactatag gctggtgctt tggcgggggc tggagtatgc aaacagcaat gatgcttgat
    18181 aaacaatgta aaggctgtat tatttattat ggcatgcccg aaaaagataa agaaaaatta
    18241 atgacaataa atttccccat ccttggaata tttgctcaaa aagaccaatg gattactcct
    18301 gaaatttata ataattttcg cgatgcgatg aagataaaca gcaagcaaat tgaaatagta
    18361 gaatttgatg ccgaccatgc ttttgcaaat ccaagtaacc cacagttcaa tcaggaattt
    18421 actacaaagg ctaatgatgc tgtttacaat ttcattaaaa cctttttcga ataaaacttt
    18481 cattaaattt tatcgcgagg ttttattttc ttctaatggc acaaataaat tatgtttaaa
    18541 aaatctgaag tttggtttgg aataataagc ggattgctaa tgcctcttgt tggtattttt
    18601 ttggttcgaa taaccttctt tccaaaccag gatataaata aaatattaga attcttctta
    18661 atggatatgg atacaatgag cgatgtgctt gcgcttagct tgttggtaaa tggcggaata
    18721 ttttatttgt ttctcaagta cgataaagaa gaaagcgcac gcggtgtggt attgtctacc
    18781 tttttgtggg gcattgtttg tgtgtgtgtg aagttttttg tgtagtaagg gggagtttgg
    18841 aagttcaaga gtttaatagt tcaagagttt tgaaagttga gaaggttttg aaagttgaga
    18901 aagttcaagg tcggaaagta caatatttaa tagctgcgaa aagcttgcgg tgagtattct
    18961 caaactgata gaaagtaact agagtttcct atgattaaag ccccttgtag gagcctgccc
    19021 cgaccgaagt gtcggggttg gggtgcgttt aattttttca cgaagtatgc attgccgttc
    19081 ggctttagcc aacggaatta caggtgttgg ggaaagagtg ttatggtgtc aatttttgaa
    19141 taaaatttaa gtgttatatg gtttctcaaa aaaaatcaat tttaaatttt gcaatgcact
    19201 aaatgggatg tatcaagtac gaataaaatg ttccaaattc gggtataata tttaactttg
    19261 aaacaaaatt ataataaaga aaatggcaaa ttataaaatc aaacatttgt tagtccaaaa
    19321 tattggggtt ttcaaacatt tagatttaca atttcctgag tcatccgtag acggtaatgc
    19381 cgacattcat atttttattg gagacaacgg cacgggaaaa actactttgt tggaggcttt
    19441 aacttatttt gacaaagtat ataatcttgc atttagaaat aaggaagata aatcatatag
    19501 tttgcttatt gaaaaagcat atcagaataa atactatctc tcaattggtt atgaacctga
    19561 aatagaaata atctttggtg atgccaatat caaatttaaa attaaaagtg gtaccagtct
    19621 tactagtcct gaagacgagt ctttttttga atcctacttt tccgattttt taaacccaaa
    19681 caactattat catacagact attcgttttt cgcatataac ggtcaccgcc aaatatctga
    19741 tactaaagtt gaatcattta aggaattaga atttaatccg attgcagatg ctttgtcatt
    19801 taataaaagc aatttaaact caaatatttt tcaatggatt gccaataata aaacccgtgc
    19861 tgcactatct tttcaagaag gaaagaaaag tgattcggaa aaatatttgc gtaccataca
    19921 aaaagtggaa gatgcgattg gaaatatcat tgatcgaaaa gttgagtttg taatcgatgt
    19981 gcattcttta aaagtaatga taaatctgga taatgactcg attaattacg aaacactggc
    20041 agatggttat aaatcagtta taagttggct taccgattta atgtttcgtt tagattattt
    20101 atctaccgaa gaaaatcctg cctttacttt attcctagac gagatagatg tgcatttaca
    20161 tcctgcagcg caaagaagaa ttttaccggt tattcaaaaa ttatttccca atgcgcaaat
    20221 atttttaaca acacattcgc catttataat tggttcggtt gatggtgcaa gagtttacaa
    20281 atttaaactc gatgagaatg gccattcggt tttagatggt gaacctttcg aaacagaaga
    20341 tggcaatagt tatgaatata ttttggagaa tctattcgat gttcataatc catttggtgt
    20401 tgaaatagaa aagcagttga aagaatttta taacaaacga aatgagctta tcaaaaatac
    20461 aaccgattta ggtgttgatg aacttgttgc ctcatcacgt gcgctggcag aacaaagtga
    20521 tgaattacga acattgattg gaatcgaaat gcgtcaactt tctcgcataa taaaaaagga
    20581 tattgtatta taagttatgc ggaaatttaa aagggctgaa tcgccagatt ttttaacaaa
    20641 aaaatggcat gagtggggca atagctacaa agaaaataaa actaaaaatt caggatttac
    20701 atttcagtgg ccaaatcatg aatcaataaa gataaatgta atactcaaac cattgcttgc
    20761 tcagcttaca ggcaatcatt gttcttactg tgatgattat ccattaaaaa caaaagctga
    20821 ttctatagat cattttaaac caaaatctcg atcggaattt tttgaactag tttgtcagtg
    20881 ggaaaattta ttctattgct gcaccaattg tcaaaacagt aaaaaagaga aattccatgt
    20941 tcatttactc cgagctgaca gggtggattt ttctttttcg aaatatttca tttatgatta
    21001 tactaatcac aaaattgaac ctaacccttt tgcagaaaaa atagagcaag aaagcgccac
    21061 aatttctatt gaaatatttg gtcttaatga tcccggacac gtttcttctc gaagaatttc
    21121 tttaaacagg tataattcac aacccaaaga caaaagtatt ttcatagacg attttcctta
    21181 tcggtatatt ttagaaaata tttaaccgat gaaatactac ataatagccg gtgaggcatc
    21241 gggcgatttg catggagcaa atttactcaa gcatctgaaa cagaaagacg ctcatgcaaa
    21301 ctatcgtgca tggggtggtg atatgatgca agagcaaggc gctgagattg ttaagcatta
    21361 ccgcgattta gcattcatgg gttttgccga agtgattgca aacttgcgca ccattttgcg
    21421 taatatagaa ttttgcaaaa acgatatatt gcaatacaaa cccgattgcc tgattcttat
    21481 cgactatccg ggttttaatt tacgtattgc cgagtttgcc caccagcaaa atttgaaagt
    21541 ggtgtattat atttcgccac aagtgtgggc atggaaaaaa tcgcgcgtgt ataaaataaa
    21601 aaagtttgtc gataagatgt tggtcattct tcctttcgaa aaagattttt ataaaaagta
    21661 cgattatgaa accacttttg ttgggcaccc tttattagat gctattgaaa attatcgcaa
    21721 taaaagatta tcaccctcac aaataaaaca gccttacatt gcattgctgc ccggcagccg
    21781 catgcaagaa attaaagtga tgttgccatt gatgttggaa gcggccatgc aaataaaaaa
    21841 ttatgaaatt gcaattgccg gagcgccttc tattgatgca acattttatg aatctatgat
    21901 ttcaaattca ggcgcaaaaa ttatttacaa caacacttat catcttttac aatatgctca
    21961 tgcagccttt gttacttcgg gtacagccac cctcgaaacg gctttgtttc gtgtgcctca
    22021 ggtagtttgt tacaaaggca actggatatc ggtacaaatt gcaaggcgat taatcgatat
    22081 acgctttata tcgctggtaa atttaattgc cgataaagca attgtaaaag aattaataca
    22141 acaggaactc accacacaga acatggtaag cgaaatgcaa aaattattgc agggcgaaaa
    22201 gcgaaacaat atgttaaggg aatatgacaa actgatagca cttttaggaa atgccggtgc
    22261 atcggaaaaa gccgcacaag aaattttcga ttttttgaat gggatggatt gaaatgggat
    22321 ggattttaat ccataaagtt gtgcaaccat aaccgtatac gatggagttt ttaatttgcc
    22381 aacgcattag aagtattcaa cattttttgt aaaattttat ttgcactgtc aatacttcca
    22441 atattgttaa caacgagcga aggctttcct ttatcgtctt tcaacttgca ttgatgcgca
    22501 tgcgaattta caaaaccaat catgccgcta aaaagaggtg attgaaaaaa tttatcatcg
    22561 ttaacaaagt atcctttaaa caaattattc tttaaaaata ttttttcgaa accaagcgcc
    22621 ttggctttcc atcgcaacct tatagtatcc atcaactcta ttgccgggcg gggcacaggg
    22681 ccaaacctat cgtgaagcat ggttgcaaac tcttccagtt tttgctcctc ggtaatatcg
    22741 tcaagctctt tatacaaatt caggcgctcg gtaatgcttg taacataatc ttgtggcaac
    22801 aatatttcga ggtcggtatc tatttgacaa tcgcgcacaa attcttcttt ggcatactcc
    22861 tccttaaact catctttaaa atctgtatgc ttcaattcga aaatggcttc gtccaatatt
    22921 ttatgaaacg tttcgaaacc gatgtcggca ataaaaccac tttgctcacc accaagcatg
    22981 ttaccggcac cacgtatatc caggtcgcgc atggcaacat taaatcccga accgagatcg
    23041 ctaaattctt caatggcttt taaccgttgg cgtgcctcgc ctgtaagtgt tgtaagcggt
    23101 ggtgtgagta aatagcaaaa tgctttttta ttgctacgcc ctaccctacc acgcatttga
    23161 tgcaaatcgc tcaagccaaa attttgtgcc tgatttatta tcatcgtatt tgcgttcgat
    23221 atatcaagtc ccgattcgat tattgttgtg gaaaccaata catctgtatc gccttcaata
    23281 aaacccagca aaacttcttc gagcaaatca ccttccattt gcccatgacc cacagcaacc
    23341 cgaacgttag gacaaagcct tttaattttt tcggcaagat cgtgtatatc acttattttg
    23401 ttgtgcacca caaacacttg tccaccacgc gatacctcaa acatcactgc atcttttatc
    23461 actttatcgg tatgcatatg aagttcggta gttacaggaa agcgattggg aggtggcgta
    23521 ttaatgatac ttaaatcgcg agcgcccatg agtgaaaatt gcagtgtgcg cggaattggt
    23581 gttgctgtta atgtcagtgt atcaacacta acgcgcagtt cttttaattt ttcttttgcg
    23641 cccactccaa atttttgttc ctcgtctatc accaacaaac ctatatcatc aaacttcaca
    23701 tctttgctca acaacctgtg tgtgccaata agaatatcta tttttttctc cttcagtttt
    23761 attaatgttt ctttttgctg tgcagaagat ttaaaacgat ttatataatc tatggttaca
    23821 ggaaaatctt ttaaccgctc gctaaaagtg cgataatgtt gaagcgcaag aatagttgtt
    23881 ggcaccagca cacctacttg cttaccatcg cacacagcct tgaaagccgc acgtatagca
    23941 atttctgttt tgccaaaacc cacatcgcca catatcaatc tgtccatagg atgaggtttt
    24001 tccatgtcag cttttacatc ggctgtcgac tttacctgat cgggagtgtc ctcgtaaata
    24061 aatgatgctt caagttctgt ttgcaaataa gtgtcgggtg taaaagcaaa acctttttga
    24121 atttttcgct ttgcatacaa ggcaattaat tccttggcaa tatcctttac tttctttttt
    24181 gttttttgct ttagcacact ccatgacgaa gtaccaagtt tatttagaga aggtgctgta
    24241 ccatctttgc ccgagtaccg tgctatgcga tgcaacgaat gaatgctgat atataaaata
    24301 tcgttgtcct tatatagaag acgaatagct tcctgtgttt ttccattggc ctcaatggtt
    24361 tctaaaccac caaaacgccc cacaccatga tcgatgtgtg tgatgtaatc tcccgggcgc
    24421 aacgcataca aatctttgag tgaaatagaa tcgcctttgg caaaattttt cttcatcctg
    24481 aaccgatgat agcgatcgaa tatctgatga tcggtatagc atgcaagttt caaatcgtta
    24541 tcgacaaatc cttcgtgaag cgaaagcagc aaggttgtaa aatttatatt ctgtcctttt
    24601 gctaaaaatg tttttcctat atcatcaaat atggcataga tgcgttcaat ctgttttggt
    24661 tgatcggcaa ttatcaaact ctcaatatgc tgtgtttgat tttcgtgaat ggtttgaaaa
    24721 agcaaattga aatttttgtt gaacgatggt tgtgcttgta cattaaaatg caacttcgca
    24781 gcatcatcaa aacttgcacg attaccaaac tcaacaatct tgaattgctg catcaactcc
    24841 tgcatttcca aaacaccgct gaaattttct ataggtggtg tgtcggcctc ttctacagta
    24901 ataataagct cactcatttt attgtgcaat gcggatatac tttcgcaagc cgaatcataa
    24961 tcgcgcatcc atattcgata gttgttacca ataaaattta agaagtgttc tttggtttgc
    25021 ttaagtaatt ttgtttgaat gttgggaata atacttatca catccatctg ctgcaccgaa
    25081 agttggttgg aaggatcgaa cgtgcggatg ctttcaattc tatcatcaaa aaactcaatc
    25141 cgaaaaggca attcgaatgc aaatgaatat acgtccataa tgccaccacg catggcatat
    25201 tgcccggctt cgtaaacaaa atcggtttgc acaaaatcgt tatcatccag aaattcggaa
    25261 agaaaatcct gcgacatcac tgaacctctt tttacatcaa aagtattttg atgtaagctg
    25321 tgttttgttg tcaccttttc gtaaaacgat tcggtgtgag taataatcaa attgccagcg
    25381 ggatttttac ttatgcgatt taaaacttcg gcacgctgta gcacattact attgtcgagt
    25441 gatgttgggt cgcccgtttt tttatacgat gcaggaaaaa acattacatg tgttgtgccc
    25501 aataaatttt cgaggtcgtt agcaaagtaa gctgcctcct ctctatcatt caaaacaaaa
    25561 acggcattaa atttttcgtg cttatataac gcagcaatca acaatgcaat gctactgccc
    25621 cgcaatccat ctaaatgcac atgttgcacc tcggtggtat tgtgattctt taccaactca
    25681 ccaaacaaag gatgggcgct atattttttt attaactgat ctaaattcat ttttcacttc
    25741 caacactaca aatagattat gcccgaaata taaattccgg gctttgagtg caaagatagt
    25801 tattggttgc ttaattaatt agttgatgag ttttttgttt gaagttgtta gatgcggtaa
    25861 cgataaaaca aatttaaaaa atcggaatcg ttctgcaatt acctaagaac aaacagcttc
    25921 cttgctggtt tcttaatctt aaatttgtga actcaagcaa aatacttgat aagcattaaa
    25981 ctatcttcaa ataatttcat cgaaccggaa taattattta ctttcacact attaaatttt
    26041 aaaaaaaatg aaaaaagcaa ttggtctact attcgttgca agcacattat ttttttcttg
    26101 taaaaaagaa tcgcagcaag aaataaaaaa cattgaaata aatgttacca tgaaaatgaa
    26161 ttccacttac agcaacaatg taatacatcc aaacgatcag gatgaagatg atatagtgca
    26221 tataacaaaa gatacacagc acaatgcaaa aactacttta gtaattaatg ccgacaataa
    26281 atcatcctta tttacctaca ctccacaatc cgatcaaata ggcaccgacc aggtaataat
    26341 aacaggtggc gatgcagagg aagatcacca gggccattgt ggcaataata gtggtggaaa
    26401 aggacatcac gcaaaaggca agatgggtgg ccatcacgaa gaagttgtta ctaattacat
    26461 cttcaatatt actattgtgc aataattgat tgtttaagaa cgactaaaaa gcctgcaaag
    26521 ttttgcaggc tttttttttg gtcacccgtc attgcgagcg aagcgaagca atcccttttt
    26581 cctcccgtca ttgcgagcga agcgaagcaa tcccagccgc gaaatttcgt tgcgatgttg
    26641 agattgcttc agtcgttcct ccttcgcaat gacggaaaga aatgagagtc atgtcgagca
    26701 aag
//
LOCUS       DAORQA010000018        26157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_444_length_26157_cov_26.451077, whole genome shotgun sequence.
ACCESSION   DAORQA010000018 DAORQA010000000
VERSION     DAORQA010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 26157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26157
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_444_length_26157_cov_26.451077"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(466..852)
                     /locus_tag="PK736_02815"
     CDS             complement(466..852)
                     /locus_tag="PK736_02815"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRC32356.1"
                     /translation="MNVHEFLNDCKVKVWQGTYSIVKATLIKGEYFAAISDFNEFTLI
                     QKQEHVEVDNVIQEEKGWRVLSFDTTLPFQMIGFITSVSRELADKQISIFAMSAYSTE
                     HILVKEHDLDKAVTTLKELGCVVDFF"
     gene            1266..2714
                     /locus_tag="PK736_02820"
     CDS             1266..2714
                     /locus_tag="PK736_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC32357.1"
                     /translation="MKEKIKLIGREKETASIDKLMHSGRAEFLAVYGRRRVGKTFLIR
                     QYLKNNMVFDFTGTKDGAKEQQLINFFEEYTKRAGNRKKKQVPLTWHEAFGYLASYLR
                     QLSKRKIKHVVFIDEMPWIDTPKSEFISALEFLWNQHLSKMDNVLLIACGSASSWIKK
                     KLIKSRGGLYNRVTHRIHLKPFNLFETELFLNNLGIKLSRYQVLEIYMAMGGIPFYLN
                     EISKGKSAAQFIDSTCFVAGGLLHDEYSQLYHSLFKNADNHIHIIEALASRPQGMART
                     EIASKIKISEGSLSRAMEELIDCDFVSKYQPLIHKKKTSVYKLTDAYSLFYLKFIKTH
                     KGSGKGTWEQLYKSSSFQAWSGYAFENIWLVHPSQLRRALGISGVYTILSSWKFTGND
                     ALPGAQIDMIIDRADNTIHLCEAKFTKENFAITKNYGEQLKLKKTILKQATQTKKTIF
                     TTLLTTYPAMRNKYYLEEIENEITMDKLFEPN"
     gene            3275..>3480
                     /locus_tag="PK736_02825"
     CDS             3275..>3480
                     /locus_tag="PK736_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HRC32358.1"
                     /translation="MEVKDSNGNILNNGDSVNVIKSLKVKGLSTDIKRGQLVKNIKLT
                     DNEKEIEGKVNGVQLVLKTEFLRK"
     assembly_gap    3481..3580
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3709..4701
                     /locus_tag="PK736_02830"
     CDS             3709..4701
                     /locus_tag="PK736_02830"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRC32359.1"
                     /translation="MQKIGKSTFAWRVIEVLQGVSFSTNYLMFYKWHLLKKTSINEIN
                     IEFVSYCNLRCKLCSLDHEKPKVRMSAQTLKHFFEDLLSDKRFRSVQTIHLYNAGEVL
                     LHPKLEEMLAIIKHYKQEAQRTGKHFPKVALLTNATILDEENSSIIINSGVLDNIRFS
                     MDGGNKEKFEEMRVRAKWDTFVKNITTFCELNNKSAKPIPTGIITLVEYENKLNTTWM
                     SNEFRKLLDMVSGYELRYAHNWGGDVEIQELKNKKKKEYKVGCSLLMHQLVLLPNGDI
                     TVCCADLNSKGVIGNILKEKLHTIYMKPQRLEMLNKFMKGKKSEIDLCKDCETF"
     gene            complement(4886..6064)
                     /locus_tag="PK736_02835"
     CDS             complement(4886..6064)
                     /locus_tag="PK736_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC32360.1"
                     /translation="MATDRFQGVDYFQIDELLTEEHILVRNSVRDWVKREVSPIIEDY
                     AQRAEFPKQIIKGLADIGAFGPYIPAEYGGGGMDQISYGLIMQEIERGDSGVRSTASV
                     QSSLVMYPIYTYGSEEQRKKYLPGLAKGDLMGCFGLTEPDHGSNPSGMLSNIKDAGSH
                     YILNGSKMWISNAPFADIAVVWAKDEAGDIRGMVIERGMEGFTTPETHGKWSLRASAT
                     GELVFDNVKVPKENIFPNVKGLKGPLGCLNSARYGIAWGAIGAAMDCYDSALRYSKER
                     IQFGKPIGGFQLTQKKLAEMVTEITKAQLLAWRLGVLKNENRASAQQISMAKRNNVNM
                     ALQIAREARQIHGGMGITGEYPIMRHMMNLESVITYEGTHDIHLLITGMDVTDINAFN
                     "
     gene            6594..7790
                     /gene="atpB"
                     /locus_tag="PK736_02840"
     CDS             6594..7790
                     /gene="atpB"
                     /locus_tag="PK736_02840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRC32361.1"
                     /translation="MISRIKSAYTRFFTYSLALLFALAFTTTQAQDGHNAIQELDNGT
                     AKTHEAEGEHDVTKGEHGAAEKFDAGKMILEHISDAHSWHLWGHTAIPLPVIVYNKDR
                     GGLKIFSSSHFDHGHSAYEGYMEKEGKLVAVDSEGKEDEAANANLWDFSITKNVMSLL
                     VGLLILTWIIFSIAGAYKRRAGLAPKGMQSLIEPIIIFIRDDVAKASIGEKKYMKYLP
                     YLLTLFFFIWINNLLGLVPIFPGGANLTGNVAITMTLALITFFIVSFSANKNYWHHIF
                     AMPGVPKWVLFLLTPIEILGVFLRPFVLLVRLFANITAGHIIALSFFALIFIFGEMNV
                     GLGYGVSIVSLAFTVFMTCLELLVAFLQAYVFTLLTAIYIGAAVEEAHHDSHSHDAHV
                     EEAAII"
     gene            7926..8192
                     /gene="atpE"
                     /locus_tag="PK736_02845"
     CDS             7926..8192
                     /gene="atpE"
                     /locus_tag="PK736_02845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRC32362.1"
                     /translation="MLSQILLDAAVSGANVGIGYGLGALGAGMAAIGAGLGIGRIGGS
                     ALESIARQPESLGDIRSNMILAAALVEGAAFFAMVIGLLVIFLK"
     gene            8339..8833
                     /gene="atpF"
                     /locus_tag="PK736_02850"
     CDS             8339..8833
                     /gene="atpF"
                     /locus_tag="PK736_02850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRC32363.1"
                     /translation="MQLVKPEIGLIVWMLISFGIIVFLLAKYAWPVILTALDEREKSI
                     TEALDSARKAKDDVAALKADNEKILAEARNVRDTMLKEARETKDKIVNEAKAKATEES
                     DRLLKLARESIQNEKMAAITELKNQVAQLSVDIAQKLLTEELSSPEKQKTLVKALLEN
                     VNLN"
     gene            8868..9425
                     /gene="atpH"
                     /locus_tag="PK736_02855"
     CDS             8868..9425
                     /gene="atpH"
                     /locus_tag="PK736_02855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRC32364.1"
                     /translation="MKNAKVAVRYSKALFGLAQELKLTDQISSDMKLISDTVNANREL
                     EMVLQSPIIKPAQKENVLTAIFNSSVNSVTIQFLAMLTRKGRSADVSNVAEAFHNRYL
                     ELKGIAKAQITTATALDADTRAAVASSIKAAIGKEIELTEKVNSDLIGGFIIRVGDKQ
                     DDTSLQTKIGKLRRAFKENPYVKDY"
     gene            9521..11101
                     /gene="atpA"
                     /locus_tag="PK736_02860"
     CDS             9521..11101
                     /gene="atpA"
                     /locus_tag="PK736_02860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRC32365.1"
                     /translation="MAEIKPAEISAILRQQLSNFKTASELEEVGTILQIGDGIARLFG
                     LQNVQSGELIEFQNGLQGIVLNLEEDNVGAVLLGPQGNLREGDTAKRTSRIASIMVGE
                     GMLGRVVDGLGAPIDGKGPITGELLEMPIERKAPGVIFRQPVTEPLQTGIKAIDAMIP
                     IGRGQRELVIGDRQTGKTAVCIDTIINQREFYDAGKPVFCIYVAIGQKGSTVANIVKT
                     LEERGAMPYTIVVSASASDPAPMQFFSPLCGAAIGEYFRDTGRPALIIYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKIINSDEIASQMNDLPPSLKGKVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLEANLFNSGVRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQAQYRELEAFSKFGSDLDAATKAVIDKGARNVEILKQGQY
                     APVTVEKQTAIIYLGTKGLLRNVPINKVKDFEADFLNVLETQHKGILDSLKAGKLDDT
                     ITGTLEKVGAELAKRYGA"
     gene            complement(11269..11664)
                     /locus_tag="PK736_02865"
     CDS             complement(11269..11664)
                     /locus_tag="PK736_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC32366.1"
                     /translation="MKHKIKTIRELKNYSQEHVSKRMNMSQSAYSKLENSETELDSQT
                     KQLVGAALDITVEQFDNFDGTQIFRITSQNNNNVDSGVINDYHDSIEYLKEMLTSKNE
                     TIEQQKQIIKIQADEILTLKSLISFIENK"
     gene            11821..12513
                     /locus_tag="PK736_02870"
     CDS             11821..12513
                     /locus_tag="PK736_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32367.1"
                     /translation="MDSTKNIWRLSFLFFVICFSIAGCKKEETTQTKYDKRMPQQMLD
                     YGYFKKGTYWIYEDTVSKAIDSVYVFDDYYIVDTLPKDNPNGLEAGIYDEFNINTHST
                     HFNVDYYYWCNSSQTPDGSRYFIQRGYTTLFIHGSTPCFRFIFEKGKVAFGYSNIPEV
                     TDTMICMNANYSEQIDKKVFTRVVKFSQTANYLENKSLTYFSYSCNIGIIKKEIIKRN
                     EYWILIRYNIIQ"
     gene            12564..17048
                     /locus_tag="PK736_02875"
     CDS             12564..17048
                     /locus_tag="PK736_02875"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32368.1"
                     /translation="MKKTAILLLWLCIAITELHSQQSQSGYMKKYWNYENRYRKYFVH
                     LGNNAGDGLPIAHRPLRRLDEQAFTYEPRDWYPDGAPDGIYDGGWKGIKFGDVTADLG
                     DYIARLATQYKLLKNASEDTLPTLNELYYAIQCVERVDLEAEFYLSQQQSPKNLNGFF
                     MRGDVEKDYHAQWNNENTQNNSQDEKDKIKGQDSPSSFTGQPQGYLNPHNEMSQDQII
                     ALMLGFAFVVKYVDNVTVQPTAADVPKNLVTQVQDITNRVMDYIQATYYNINLQLNGS
                     LPVAAFTFHNAPNQHFTINTQLDLNWSIVNPLSGQAVHEMANQYGTVIGFAYPMCAAA
                     HFITGNSYDNDGYIHCNNFVIPTFPPKVYSNMNVNIPFNELRQGWNDIPNGIGPILSV
                     INPINDYTCVNYSALKTIILPPYLLPVNGDFCIRNWILNGIYAGSFEKDYNLNLALSL
                     ASTTSSSYTNWSHNEIKTFAQDGNMEIFDLIYATLHDVAPLIPKIEYEMIINSAPDEG
                     PYSYDNSDYSLGWSENSRWSHPFKISSDPGGKNGNDYMWLYNLYRLAFASDGDVASEA
                     YNDRQTNPVEFYYCPAIVKNTFTNSQQWLEVNNSFSINRKFKNYLDFKCGIKEYTSKN
                     TTIKSQYTMQIKTDLVFPTFSYFDVDGGKIEVGEPTNNFYDQGNIIMHQGDLYFHANG
                     QCEIFGNGKIIVEAASLNLGGSSKTILHSGAEIIIKSGGSLVFGQNSQIILEDGAKIT
                     VENGGELRYYNDVDLQLKGDNAVLEIAGKLDIQDNATFTFTYPGANSGYVKFSNVETD
                     IYTPTENINYGTNSKINIHGQGKTDKVLEVTQESIYTSGAANGTCAEFRVTDGKVEMS
                     TNARLNLDCKVFINNATIASSFAPGTSIARGIDVFGQPSVSISNSDFIGLQKGINGLL
                     FYGGKSLSVPGCSFIQCDRGLNIEGATVYVNACTFIENGIGVKIQNITKPAYIRHSNF
                     TVNDYNTGISASVATGTPLNVWFCNINGGPYEMNELTGINIDGGNLNLKCTNIHYMRY
                     GVKANDNSFVNMSAENGFGYNDLSNNETAIAFQKCDGVNFKNGHNNFSFNPNLLSGYY
                     NPNNTWVTNSCSYCNFNGLPRMSGEFTHPPCFNNGGTLIFECDQNQWNINDVIGPAMS
                     TQTYQATNTYDRYDVGYVCSNNSHVADVIFADPNPSAAQACPPGSSDCVNPPCLAISN
                     IDVSTLRVINTPTFQNQEMEVAINNATDNINETADGNRSTIMLLKEILDYNLTNPTQE
                     EVRVLDYSIRTIQMALANLQLLKTEVSDTIEVESYFTATLQLLDNKIAQAVAENNYYH
                     HVYLMFDKACVQHLHGDRMAAISTIEQIQLFASAEEVDYLAYWHCLYDLENQAITGVI
                     TKEEYTNTLEMCAPPQYLRVMNISENQLEREVKHNPYHFNVDNTNSMAQLKFNSQIAE
                     NTTISVYDLSGKIVSSQSFDTQKGENNFVINLANLSKSIYVVQFKVNENVCREKAGLY
                     "
     gene            complement(17229..18530)
                     /locus_tag="PK736_02880"
     CDS             complement(17229..18530)
                     /locus_tag="PK736_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRC32369.1"
                     /translation="MSQCRTGKLLFPYIMFNFSHVFKCVFILLLFNFCIQQKSIAQPV
                     SKTRNDLEKQKNELLREIEETNAQLKLNEKNQKATKSQLAQLAKQLRTREQLIRTINN
                     QIGNLGSEIEQTEQEIANLESEMMQLKAEYARMITFAQRNRSSYQKMMYIFASKDFLQ
                     ATKRIKYFQQYSDARKVQAFRIDSVQKELTIRQTFLQNQRQIKSKLKTTEEQQKQNIA
                     EEKHAQEGFMKNLSKQESVLRKRLAEKEATKKKLENAIAENIRKEIESVRKKAEAGGK
                     KKDDITSKNAFSQTPEELKLSNSFASNRGVLPWPVENGEVVSTFGEHQHPVLKNVTVK
                     NDGVDIQSSKGAAARCIFAGEVTGVINIPGSYAAVIVRHGEFLTVYSNLEGVLVRKGD
                     KLTTKQKIGTIHCDTETNVAELNFQIWKGFTKLDPQAWLKR"
     gene            complement(18508..19395)
                     /locus_tag="PK736_02885"
     CDS             complement(18508..19395)
                     /locus_tag="PK736_02885"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HRC32370.1"
                     /translation="MNKVISFPTRNVVFTFHRVLLLLFLFAAFAACKSTRKVTTKTVT
                     VPIAFDTINYQKFSSSSLIDSLYKYRFSAVTMSAKADVAVIDTNDKKEFNANIRIQND
                     SAIWISINAILGIEVARILITTDSIFVMDRLNKKYTKADFKYLNDLLKLKVDYNTIQN
                     IIIGNFFQYRNENKFNSVYIEDKGYILSTLNKRKLKRSLEDKDPNKPIIQDFYVNPAN
                     YKVDQLQITDDKLNKTLTTDYNSFEVTEAGVFPMLLNTQVSADKKLSIVIQYNKVTVN
                     LPVEMPFNIPQSYEPMPHR"
     gene            complement(19402..21144)
                     /locus_tag="PK736_02890"
     CDS             complement(19402..21144)
                     /locus_tag="PK736_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC32371.1"
                     /translation="MYKVVNSKWYFISLCSCLVFVNVFCVNVIYAQKKKKDNVSSESR
                     MTLDRLFVDAHREKNTGSAEKAINVFKQCLSIDKHHAASMYEIAKLLRNQNKNDEALE
                     YAASAYEEDNTNEWYAMEYAELLQINNKAKPAIEVYETLVKQKPGDINYYFMLVESYM
                     QNNDNEAALKVFDKIEKRFGVDADIVKEKQRIYIKMGKLDKAAEELEKLITSDPSLEN
                     YGLLVELYQINNEEDKAYEVIQRMKKIDADDPRIALSLAQHYRAKGEKEKSYAELKKA
                     MGSKKLPAETKYKIMLSYLPLMNVDEGMKAQAKELSKIFVDTEEDDPMAHAIYADILI
                     QDKNYNEARTHLRASLKKDTKTFATWQQLMNCDLQLADWVELDKTSNQAMELFPTQPV
                     VFLYNGIANAQLKSYETAIKSLMAGSKMVVLGDPLLLDFYSSLGDNYYNVKNYPESDK
                     YYEKVLKLDPKNSSVLNNWAYYLSLRKENLDKAAEMAKKVTELNPHVATYEDTYAWVL
                     YAQGKYAEAKTILESALQNGGDKNGAILEHYGDVLFKLNDPQKALEYWQKAEQAGDAS
                     ELIKQKIYEKKLFD"
     gene            complement(21187..22188)
                     /locus_tag="PK736_02895"
     CDS             complement(21187..22188)
                     /locus_tag="PK736_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRC32372.1"
                     /translation="MKIIVPMAGMGKRMRPHTLTVPKPLIPLAGKAIVQHLMEDIVKV
                     CPEKVDEIAYVVGHFGEEAEANLKNIAASLGAVGTIHYQEEPLGTAHAILCAQSALKG
                     KVVVAFADTLFRTDANIDENSDGTIWVKQIEDPRQFGVVKLNADGVITDFVEKPQTFV
                     SDLAIIGIYYFKDGENLRSELQYLIDNDIKDKGEYQLTNAMENMKVKGKKLVPGKVTE
                     WLDCGNKDATVYTNKRVLEIKYPNNIIDKSAELINSVIIPPCYIGANVKLSNTVLGPH
                     VSIGDKCEVNNSVVRNSIIQTNTKVNDALIDNAMIGNFCQFTGKVQDLSMGDYSTQS"
     gene            complement(22266..22703)
                     /gene="dut"
                     /locus_tag="PK736_02900"
     CDS             complement(22266..22703)
                     /gene="dut"
                     /locus_tag="PK736_02900"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRC32373.1"
                     /translation="MTQVHIINKSQHVLPGYETPSSAGMDLRASIEAPMHMQPGERAL
                     VPTGLYMALPTGYEAQVRPRSGLAFKNGVTVLNAPGTIDADYRGEIKVMLINHGHEIF
                     TINNGDRIAQMIIATYAQVELKAVHTLDATERGAGGFGSTGKN"
     gene            complement(23148..24467)
                     /locus_tag="PK736_02905"
     CDS             complement(23148..24467)
                     /locus_tag="PK736_02905"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRC32374.1"
                     /translation="MAFNIIDMASGYLTNEVISKASSFLGENESSTAKAVSTAIPTLV
                     AGLMNKASDSNGASMIFNLLNSGNNNGSMLNNLSGMFGGGDETNNLVSKGAGLMDTLF
                     GSKSSGVADLLSSTSGISSKSSSSLLAMLAPVVMNLFGKEVASNGLDLRGFTSLLGSQ
                     KSFISGLIPSGLTSMLGLGSIADLGGKLFSGASNAASTVTSAASSAYQNTQEKSSGLA
                     KFSPWLLAAFIAMIALYFWKGCGKAEEHGTEATTEAAAHGEEAATSVAVAADSAAANV
                     ADEANGLWANLGKFFTRKLSTGIELNIPETGIENQLITFIEDANKPVDKTTWFNFDRL
                     LFETGSASLKPESQEQLKNMVEILKAFPGVDLKIGGYTDNTGKADANLKLSAARANNV
                     MAELVKLGVDAARLKAEGYGDQFPVATNDTEEGRAQNRRISCRVTKK"
     gene            complement(24643..>26157)
                     /locus_tag="PK736_02910"
     CDS             complement(24643..>26157)
                     /locus_tag="PK736_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HRC32375.1"
                     /translation="DLLLPHVIFLREKGYDVLTVNNGDDAIEKVRNQDFDIILLDENM
                     PGLSGLETLAQIKNLKSHLPVIMITKSEEEHIMEDAIGNKIADYLIKPVNPNQILLSL
                     KKNLDNKRLISEKSNSLYLQEFRQIGMRITDRLNYNDWVDVYKKLVYWELELERNPDQ
                     SMYEILVSQKDEANLMWSKFVENNYVSWLKTPDQGPSLSHQLFKNKVFPMADAVEEPI
                     YFVLIDNLRWDQWKIIQPVISEYFRVTEEDTYCAILPTATQYARNAIFAGMMPSEIEK
                     RFPKMWFNDEEDEGKNLYEPDFLADQLQRNRKNYKFSYTKITNHQSGKELVENIHRMA
                     DNKLNVIVYNFVDMLSHARTEMEMIRELAENEKSYRSITLSWFMNSPLFEALKRVSEK
                     PCKLIITTDHGTIRVKQAVKIVGDKNTNSNLRYKNGKSLSYDKKEVFEIKNPADAMLP
                     RQHVSSSFVFAKSDDFFAYPNNYNYYVTYYRDTFQHGGVSLEEMIVPIISLSSK"
BASE COUNT         8012 a         4778 c         4975 g         8292 t
ORIGIN      
        1 cagaataaaa aatgctgcgc aaggtgggtt gtttgattct taaattcttg tcattggtca
       61 ttgtcacctt gagcgaagtc gaaagggtca ttggtcattg tgaaatagtt attgattatt
      121 gagctattgg gttgttgact ttgtcatgct gcggaacgaa gcatctctgc cagtcattgg
      181 tcatttttag ttctggtcat tgagaattgt caatggtcat tatcgtcatt gcaagttacg
      241 aagcaatctc tgccagctat tgttcattat ccattttata caattttcac tttcattact
      301 ctcaattcat gccataaaac aatcgttgca attaaattct gactaagttt tttctgatcc
      361 attgagtggt catctaacga taagcaaaaa caaacctgat ttttttaatc gtatttgatg
      421 gtgaaaaaag accgtgatta ataaaccgtt acatcttgct ttcgactaaa aaaaatcaac
      481 tacacaaccc aactctttta gtgtggtaac tgctttatcg aggtcgtgtt cctttaccaa
      541 aatatgctcg gtagaatatg ctgacatggc aaaaatagaa atttgtttat cggccaattc
      601 gcgcgacaca cttgttataa agcctatcat ttgaaacggt agcgtggtat cgaacgacag
      661 cacacgccag cctttttctt cctgaattac attatctact tcaacatgtt cttgcttttg
      721 tatcaatgta aactcattga aatcgctgat ggcagcaaaa tattctcctt taataagtgt
      781 agcctttaca atagaatatg tgccctgcca tacttttacc ttacaatcat ttaaaaattc
      841 gtgtacattc attgtgttta tgtttatggc cacaaattaa atattttttt gccaatccaa
      901 cacattactc tttaattgtt atgcaacttg ttttataagt tgaacaaacc gatttgtcta
      961 ataacctttg acatgcacag aaataatacc aaaaccataa atgatgatat agttttgagc
     1021 atcgaaactg tgtaaatctt ttctctgaaa tttacattta tttatatgga ttcaggcaaa
     1081 gtgtttcgca aaaagtgatg aatgtataat tggagaaatg tatgttttta taatacattt
     1141 ctccacggat ataaaatact gtattataaa ataaaagtaa tttatgttga aattaaattg
     1201 cgtattcaaa gtgcatactt ggagaaatgt agtatatttg catagatttc tccaagtata
     1261 aaaaaatgaa agaaaaaata aaactcatag gtcgcgaaaa agaaacagca tcaatcgaca
     1321 aattaatgca ctcgggaagg gcagagtttt tagcagtata cggccgcagg cgagtaggca
     1381 aaacattctt gatacggcaa tatttaaaaa ataacatggt gtttgatttt accggtacaa
     1441 aggacggagc aaaagagcaa cagttgataa atttttttga agagtatacg aaaagagcag
     1501 gcaacagaaa aaaaaagcaa gtgccattaa catggcacga agcattcgga tatttagcat
     1561 cttacttacg gcaattatct aagagaaaaa taaaacacgt ggtttttatt gatgaaatgc
     1621 cttggattga cacaccaaaa tcggagttta tttcagctct ggaattttta tggaatcagc
     1681 atttatctaa aatggacaat gtattactta tagcatgtgg ttctgcatca tcgtggataa
     1741 agaaaaagct tattaaatcg cgtggtggat tatataaccg ggtaacacat agaatccatt
     1801 tgaaaccatt caacctattt gaaacagaat tatttttaaa taacttagga ataaaattat
     1861 cgcgctatca ggtattggaa atatatatgg caatgggagg cattccattc tatttaaacg
     1921 aaataagtaa agggaaaagt gctgcgcagt ttattgattc tacttgtttt gttgcaggag
     1981 ggttgctaca tgatgaatac tctcaattgt atcattcact atttaaaaat gccgacaatc
     2041 atatacacat tatcgaagca cttgcatcgc ggccgcaagg aatggcgaga actgaaatag
     2101 catcgaaaat aaaaataagc gaaggttcgc tatctcgtgc tatggaagaa ctaatagatt
     2161 gtgatttcgt ttccaaatat cagccactca tccacaagaa aaaaacatca gtttataaac
     2221 ttactgatgc atactcgctg ttttatttga aatttataaa aacacataaa ggaagtggta
     2281 aaggtacctg ggaacaattg tacaaaagca gctcgtttca ggcttggagt ggatatgcat
     2341 ttgaaaatat ttggttggtt cacccttcgc aattaagacg cgcacttggt ataagtggcg
     2401 tttacactat tttgagttcg tggaaattta cgggaaacga tgcattacct ggtgcacaaa
     2461 tagacatgat tattgacaga gcagataaca ccattcattt atgtgaagct aaattcacta
     2521 aagaaaattt tgcaataact aagaactatg gcgagcaatt aaaactaaaa aaaacaatct
     2581 taaaacaggc tacacaaact aaaaaaacaa tctttactac cttgttaacc acatatcctg
     2641 caatgcgaaa taaatattat ctggaagaga ttgagaatga aataacaatg gacaaattat
     2701 ttgaacccaa ctaatcttaa ctaacattgc aaaaggttgg agtacgaagt tgattatcga
     2761 aacgttttat ctttttgtgg aatagaattg ttggagaatg aaagaataga attgttggag
     2821 acaactccaa taatggggca atgtcacaac acccggtgtg gctgcttttc cctcattggg
     2881 aaacggtcgc aacgatatgg gatgctacgg aggcttgtat gctatggtaa taccctcatg
     2941 tgaaaatact tcttatgggc ttaatgaagt aaaacaaaaa attccatatc aattatttcc
     3001 tgtgccgccc aattcgcaaa tgactataca ttttgatgat gtattgaacc gcgaaattta
     3061 tttggaaatt ttcgcttcga atgagcaaaa tattttcaga ggaacaataa cagaaagcga
     3121 tactaaattg aatgttgaat cttataagaa tggaatattt gatgcatgtg tattcttccg
     3181 aaaaggacta cagcccaagg cgatttcaaa tcgttcatta atttttcgat ggcaagcaaa
     3241 aactattttt gaatatcttc gcagaaaaaa gaccatggaa gtaaaagaca gcaacggaaa
     3301 tattttgaat aatggcgatt cggtaaatgt gataaaaagt ctgaaggtaa aaggactatc
     3361 cacagatata aaacgtggac aacttgtaaa aaatattaaa ctcaccgata acgaaaaaga
     3421 aattgaagga aaagtaaacg gagtacaact ggtgctgaaa acagaatttt tgcgcaagtg
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaaattaaat gaattgtttt
     3601 actgacaaat atcatgtttt atattgccaa tgagctgtat ccttgccacc aaattgctat
     3661 gctatgttcg gcttggatat aaatacgttt gaagtaaaaa atttttttgt gcaaaaaata
     3721 ggcaagagta cttttgcatg gcgcgttatt gaagtattgc aaggagtttc gttcagcaca
     3781 aattatttga tgttttataa atggcatttg ctaaaaaaaa cttccatcaa cgaaatcaat
     3841 attgaatttg taagttattg caacctgcgt tgtaagttgt gctcgctcga tcacgaaaaa
     3901 cccaaagtgc gtatgagtgc gcaaacgttg aaacattttt ttgaagactt actcagcgat
     3961 aaaagatttc gcagcgtaca aactattcat ctttacaatg ccggtgaagt attgctgcat
     4021 cctaaactcg aagaaatgtt ggcaattata aagcactata aacaggaagc acaaagaaca
     4081 ggaaagcatt ttccaaaagt agcgctgctc actaatgcca caattcttga cgaagaaaat
     4141 tcgagcatca ttatcaattc gggagtgctc gacaatattc gttttagtat ggatggaggc
     4201 aataaagaaa aatttgaaga gatgcgtgta cgcgctaagt gggatacgtt tgtaaaaaat
     4261 attaccacct tttgcgaatt gaataataaa agtgccaagc ccattcctac aggaataata
     4321 acactggtag aatacgaaaa caaacttaat acaacatgga tgagtaacga gtttaggaaa
     4381 ttgctcgata tggtgagtgg ttacgagttg cgctacgcac acaattgggg cggtgatgtg
     4441 gagatacagg aattgaaaaa caaaaagaaa aaagaatata aagtaggatg cagtttgctt
     4501 atgcatcaac ttgttttgct ccccaatggt gatataactg tgtgttgtgc cgatttaaat
     4561 tcgaaaggtg tgattggaaa tatactgaaa gaaaaactac atacaattta tatgaaaccc
     4621 caacgccttg aaatgttgaa taaatttatg aaaggcaaaa aaagtgaaat tgatttgtgt
     4681 aaggattgcg aaacattcta gcattttaga ttgagagata attgcaacgg agtgggattg
     4741 ttggagaaaa ctcatggatg aattgttgga gaaaacattc tcggtcagcc tgaaaactcc
     4801 aacaacggag gaatatttaa taaatcaaca aaaatgcccg ccaataatat gtggcgggca
     4861 tttttactat tataacaatg actaattaat tgaaggcatt gatatcggta acatccatac
     4921 cggtgatgag caaatgtata tcgtgtgtgc cttcgtaggt gattacgctt tcgaggttca
     4981 tcatgtggcg cataattgga tactcgcctg taatgcccat tccaccatgt atttgtcgtg
     5041 cttcgcgtgc tatctgcaat gccatgttta cattgttgcg ctttgccatc gatatctgtt
     5101 gtgccgatgc acgattttcg tttttcaaga caccaagtcg ccatgcaagc aattgtgctt
     5161 tggtaatttc tgttaccatt tcggccaatt ttttttgtgt gagctgaaat cctccaatag
     5221 gcttgccaaa ttgtatgcgc tctttgctat atcgcaatgc gctatcgtaa caatccatag
     5281 ccgcgccaat tgcaccccac gcaataccat aacgggcact gttgaggcaa ccaagcgggc
     5341 ctttgagtcc tttcacgttt ggaaaaatat tttctttagg aacttttaca ttatcgaaaa
     5401 caagctcgcc tgtggccgaa gcacgcaagc tccatttgcc atgagtttcg ggcgtggtga
     5461 agccttccat accgcgttct ataaccatac cgcgtatatc accggcttcg tctttggccc
     5521 acactacagc tatatcggca aaaggggcat tgcttatcca cattttgctg ccattgagta
     5581 tgtaatgcga accggcatct tttatattac taagcatgcc cgaaggatta ctaccgtggt
     5641 cgggttcagt taaaccaaag caacccatca gatcgccttt ggcaaggccg ggtaaatatt
     5701 ttttgcgttg ctcttcgcta ccataagtat agataggata catcaccaat gagctttgaa
     5761 cgctggctgt tgatcgcaca ccgctatcgc cacgctcaat ctcttgcata atgagaccat
     5821 atgatatttg atccatacct ccaccgccat actctgcagg aatgtaaggg ccaaaggcgc
     5881 caatatcggc aaggcctttt attatttgtt ttggaaattc ggcacgttgt gcatagtctt
     5941 ctattatggg cgatacttcg cgctttaccc aatcgcgtac gctgttgcgc acaagtatat
     6001 gttcttcggt aagtagctcg tcaatttgaa aataatctac tccctgaaat ctgtctgttg
     6061 ccatttcttt attttttttg tggcgcaaaa gtaatgtttt atattttgaa aaattgggat
     6121 agagaagata gattttttct gttgaattgt ttgatgtttt ataaagaaaa acacctattt
     6181 tttgtgccaa acctttataa ttaagaagca tttataaaag gaagacgcca cctctttatg
     6241 aattaaacct aagccaatat tacatgcaaa ccatagagga tgttaaaacc tcgcgggttt
     6301 tcatttcaga aaaaacagat tctgtttaca cgaaattaat tagaaagttg ccaggctttt
     6361 tagattttaa aaaccatact tcttatataa attgcctaac agtgaaaagt attagttgat
     6421 tattcaaata aatcaaagct gtcatttcgt gtaatctgtt tgatgaattc aatttttgtg
     6481 gcatttctat tatataaaac atcgcataaa aattattgtg aatgttcgtt tggtttcgaa
     6541 aacgaaaatt ttacctttgc gcgcgaattt taaaaaccac atttttgaaa gtaatgatat
     6601 cccgcatcaa atcagcatat acaaggtttt ttacctatag tttggctttg ctttttgcat
     6661 tggcttttac tacaacgcag gcacaggatg gccacaatgc tatacaagag ctcgataacg
     6721 gtactgccaa aacgcatgaa gccgaaggag aacatgacgt aaccaaaggc gaacatggcg
     6781 cagccgaaaa atttgatgcc ggcaaaatga ttctcgagca cattagcgat gcacactcat
     6841 ggcacttgtg gggccacact gctattccat tgcctgttat agtttataat aaagaccgcg
     6901 gtggtttaaa aatattttct tcatcgcatt tcgatcatgg ccattcagct tacgaaggat
     6961 acatggaaaa agaaggtaag ttggttgcgg ttgatagtga aggaaaagaa gacgaagccg
     7021 ccaatgccaa cctttgggat ttttctatta ccaaaaatgt aatgtcattg ttggttggct
     7081 tgttaatctt aacatggata atatttagca ttgccggggc atacaagcgc agagcaggtt
     7141 tagcgccaaa aggtatgcaa tcgcttatcg aaccaattat tatttttatt cgcgatgatg
     7201 tggccaaagc ctccattggt gaaaagaaat atatgaagta ccttccgtat ttgctgacgc
     7261 tgtttttctt tatttggata aacaaccttt tgggacttgt tcctatattt ccgggtggag
     7321 caaatcttac aggcaatgtt gccattacaa tgacacttgc tttaataact tttttcattg
     7381 tgtcgtttag tgcaaataaa aattactggc accatatctt tgccatgccg ggcgtaccaa
     7441 agtgggtatt atttctcctc actccaatcg aaatattggg tgtattcctc cgccctttcg
     7501 tactccttgt ccgacttttt gcaaacatta ccgcaggtca cattatcgca ctatctttct
     7561 ttgcacttat attcatcttt ggcgaaatga atgtgggact gggttatggc gtttcgatag
     7621 tatcattggc atttacagta tttatgactt gtctcgaatt attggtagcc ttcttacaag
     7681 catatgtatt cactttgcta acggcaatat acattggtgc tgcagttgaa gaagctcatc
     7741 acgattcgca tagtcacgat gcacacgtgg aagaggcagc gataatttag ttactggtta
     7801 tttagttatt ggttattggg ttgttagttg ttgagtttta tggttgttgc acattgctaa
     7861 ttgcctattg cttattttgt tttttgactt ttaaatttaa ttcttaattt ttaattttat
     7921 atattatgtt atcacaaatt ttattagatg ctgcagtatc gggtgccaat gttggtatcg
     7981 gttatggttt aggtgcattg ggtgcaggta tggctgcgat tggtgctggt ttaggtattg
     8041 gtcgtattgg cggtagtgca ttggagtcaa ttgctcgtca accagaatct ttgggcgata
     8101 ttcgttcaaa catgattctt gcagctgccc tggtagaagg tgctgccttc ttcgccatgg
     8161 taatcggact attggtaatc ttcttgaagt aattgccaat ccgtacaaaa attaaacttg
     8221 ccccatcaac gcttggttgg gggcaggttt tttaaatttt ttctaattca aaagcatacg
     8281 cattcacaac aagaagaaag cgtattcaaa attcgaacaa cacaaaaaga aataaaaaat
     8341 gcaattagta aaacctgaaa taggattaat agtatggatg ctgatatcct ttggtatcat
     8401 tgtattcctg ttggccaaat atgcatggcc tgtaatatta actgctcttg acgagcgcga
     8461 aaaatcgata acagaggcat tggactcggc acgcaaagcc aaagacgatg tagcagcttt
     8521 aaaagccgac aacgaaaaga tattggccga agcccgcaac gtgcgtgaca ctatgcttaa
     8581 agaagcacgc gaaacaaaag acaaaattgt aaacgaggca aaggccaaag caacggaaga
     8641 atcggatcgt ttgttaaagt tggctcgcga aagcatccaa aacgaaaaga tggcggctat
     8701 taccgaatta aaaaatcagg tggcgcagct atcagttgat atagcacaga aattattgac
     8761 cgaagaatta tcgtcacccg aaaaacaaaa gacactggtg aaagcgcttc tcgaaaatgt
     8821 aaacttaaac taacagcgat ttttattaca aataaaattc atcaacaatg aaaaatgcaa
     8881 aagttgctgt tcgttactcc aaagccttat tcggtttggc gcaggagcta aaacttaccg
     8941 accaaattag cagcgatatg aaattgatta gcgatactgt aaatgccaat cgcgaattgg
     9001 aaatggtatt gcaaagccca ataataaaac ctgcacagaa agaaaatgta cttaccgcga
     9061 tttttaatag ttcggtaaat tcggttacca tacaattttt agcaatgctt acacgcaaag
     9121 gtcgttcggc cgatgtatcg aatgtagccg aagcatttca caatcgctac ctcgaactta
     9181 aaggtatcgc caaggcacaa atcactacag caacggcctt agatgccgat acacgtgcgg
     9241 cagttgcttc aagcatcaaa gctgctattg gtaaagaaat agaacttacc gaaaaagtaa
     9301 attctgattt gattggcgga tttattatac gtgtgggcga taagcaggac gatacatcgt
     9361 tacaaacaaa aattggaaaa ctacgcaggg cgtttaaaga aaatccatac gtaaaggatt
     9421 attagaaata tttgaacgtg taagcaacct aaaaacaaaa tggttgtgaa acggcaacaa
     9481 cttcattcga taaaaaataa acttcaataa taaaaacaat atggccgaaa taaaaccagc
     9541 agaaatttct gcaattttaa gacagcaatt atcaaacttc aaaactgctt cggaactcga
     9601 agaggttggt accatacttc aaataggtga tggaattgcc cgcctttttg gtttacaaaa
     9661 tgtacaaagc ggagagttga tagaatttca aaacggattg cagggaatag tactgaacct
     9721 cgaagaagac aacgtagggg cagtattact tggcccacaa ggcaatctgc gcgaaggcga
     9781 tacagcaaaa cgtacaagcc gcattgcctc cattatggtg ggcgaaggca tgcttggccg
     9841 cgttgtggat ggcttgggtg cacctattga tggcaaagga cctatcacag gcgagttgct
     9901 cgaaatgcct attgagcgca aagctccggg ggtaattttc cgtcagccgg taaccgagcc
     9961 attacaaact ggaattaaag ccatcgatgc catgattcct attggacgtg gacaacgcga
    10021 attagtaatc ggtgaccgtc aaacaggcaa aacagctgta tgtatcgata ccattattaa
    10081 tcagcgcgaa ttttacgatg caggtaaacc cgtattttgt atatatgttg ccataggtca
    10141 aaaaggatct acggttgcca atattgtaaa aacgcttgaa gaaagaggcg ccatgcctta
    10201 taccatagtt gtatcggcaa gtgcatcgga tcctgctcct atgcaatttt tctcaccatt
    10261 gtgcggtgcg gccattggcg aatatttccg agatacaggc cgtccggctt tgattattta
    10321 tgatgactta tcgaaacagg cggtggctta tcgtgaagta tctttattgc ttcgcagacc
    10381 tccgggacgt gaggcttatc caggtgacgt gttttactta catagccgtt tgttggagcg
    10441 tgccgcaaaa attatcaact ccgatgagat tgcatcacaa atgaacgacc ttcctccttc
    10501 tttaaaagga aaagtaaaag gtggtggttc gcttacagca ttacctataa tcgaaacgca
    10561 agcgggtgac gtatcggcct acattccaac aaatgtaatt tcaattaccg atggacagat
    10621 attcctcgaa gcaaacttgt ttaacagtgg tgtacgtcct gccatcaacg ttggtatcag
    10681 tgtatcgcgc gtgggaggaa atgctcaaat aaaatcgatg aaaaaagtgg caggtacatt
    10741 gaaattggat caggcacaat accgtgagtt ggaagcattt tcgaaattcg gtagcgacct
    10801 cgatgcggct acaaaagccg ttatcgacaa aggtgcacgt aatgtggaaa tattgaaaca
    10861 aggacagtat gcacctgtta cagttgaaaa acaaacagcc attatttatc ttggtacaaa
    10921 aggtttgttg cgcaatgttc ctatcaataa agtaaaagat tttgaagccg atttcctcaa
    10981 cgtactcgaa acacaacaca aaggaatact cgatagttta aaagccggca aacttgacga
    11041 taccattact ggtacgcttg aaaaagttgg tgcagaatta gcaaaacgct acggagcata
    11101 accgaccagt cgtttataat aatagagaac tccatccgca agggtggagt ttttttgtgc
    11161 gattttattt tgtgcacaga aagctctgtt gaatgcttgg tgcttggatg gttgataaag
    11221 aaactaacca taagcagaaa aaatactaaa gctaaatcaa tgtttttatt atttattttc
    11281 aataaaactt atcaaacttt taagtgtgag tatttcatcg gcttgtattt ttattatttg
    11341 tttttgttgt tctatggttt cgtttttact tgtgagcatt tctttcaaat actctatact
    11401 atcgtgataa tcatttataa caccgctgtc tacattattg ttattttgac ttgtaatacg
    11461 aaaaatttgt gtgccgtcaa aattgtcgaa ttgttcaact gtaatgtcaa gtgcagcacc
    11521 aactaattgt tttgtttgcg aatccaattc tgtttcgctg ttttccaatt ttgaataagc
    11581 cgattgcgac atgttcattc tttttgaaac atgttcctga ctgtaatttt tcaactctct
    11641 gattgttttt attttgtgct tcatacggta gttgcatttt tctcaaagga ataaattatt
    11701 cttgaattgg aataaattat tccggttttg gaatatatta ttccattttt agtaaccttg
    11761 ttacaaactt ttagtatatc tttacaacat gccaaaaatt aaattgcatt atatttaaat
    11821 atggactcca ccaaaaatat ctggcgctta tcatttcttt tttttgtaat ttgtttttca
    11881 attgccggtt gcaaaaaaga agaaacaacc caaacaaaat acgataagcg catgcctcaa
    11941 caaatgcttg actatggcta ttttaaaaaa ggcacttatt ggatatacga agatactgtt
    12001 agcaaagcaa ttgatagtgt ttatgttttt gatgattatt atatagttga cacattgcca
    12061 aaagataacc ctaacggcct cgaagcagga atatatgatg agtttaatat aaatacccat
    12121 tcaactcatt ttaatgttga ttattattat tggtgtaata gttcccagac ccctgatggc
    12181 agtcgttatt ttattcaaag gggatacact acacttttca ttcatggttc aacaccatgt
    12241 tttagattca ttttcgaaaa gggcaaagtt gcatttggtt attcgaatat tccagaagtt
    12301 acagatacta tgatttgtat gaatgctaat tattccgaac aaatagacaa aaaagtattt
    12361 acaagagttg tgaaattcag tcagactgct aattatttag aaaataaaag cttaacttat
    12421 ttctcatact catgcaacat tggaataatc aaaaaagaaa taataaaacg caacgaatat
    12481 tggatattga tacgatataa cattatacaa taatcgtttt ttgtaattag ttaacggtcc
    12541 tctaaatttt taaattttat aatatgaaaa aaacagcaat tctattgttg tggctatgta
    12601 ttgccataac cgaattacat tcgcaacaga gtcaaagtgg gtacatgaaa aagtattgga
    12661 attatgaaaa ccgataccga aagtattttg tgcaccttgg caacaatgcc ggtgatgggc
    12721 tgcccatagc gcacaggccg ttaaggcgat tggacgaaca agctttcaca tatgagcctc
    12781 gagattggta tccagatggt gctccagatg gtatttatga tggcggctgg aaagggataa
    12841 aatttggcga tgtaacagcg gacttaggcg attatattgc gcggttggca acccaataca
    12901 aactactaaa aaatgcaagt gaagatacac tacctacatt aaatgaattg tattatgcaa
    12961 tacagtgtgt tgaaagggtt gatttggaag cagaatttta tttatcgcaa caacaaagcc
    13021 ctaaaaacct caacggtttt ttcatgcgcg gagatgtaga gaaagattat catgcacaat
    13081 ggaacaatga aaacacacaa aacaattcac aagacgaaaa agacaaaata aaaggacaag
    13141 attcccctag ttcatttacc gggcagccgc aagggtatct taatccgcat aacgaaatga
    13201 gccaagatca aataattgca ttaatgttgg gttttgcttt tgttgtaaaa tatgttgata
    13261 atgttactgt gcaaccaaca gcagccgatg taccaaaaaa ccttgtaacc caagtacaag
    13321 atattacaaa tcgggtcatg gattatatac aggctacata ctataatatt aatctccaac
    13381 ttaatggctc actaccggta gctgcattta cttttcacaa tgcacctaat caacatttta
    13441 ccattaatac tcaactcgat ttgaactggt caattgtaaa cccgttaagt ggtcaagcgg
    13501 ttcatgaaat ggcaaatcaa tacggtacag tcatcggctt tgcttatcct atgtgtgctg
    13561 cggcacattt tattacagga aattcttatg acaatgatgg ctatatacac tgcaataatt
    13621 ttgtcatccc aacatttcca ccaaaggtgt attcaaatat gaatgtgaat atacctttca
    13681 acgaattgag gcaaggctgg aatgatattc ccaatgggat tggcccgatt ttatctgtaa
    13741 taaatcccat taatgattat acatgcgtaa attattccgc attaaaaact attattcttc
    13801 caccatatct attgccagtt aatggtgatt tttgtattag aaattggatt ttaaacggta
    13861 tttatgcagg aagttttgaa aaagactaca atctaaattt agctctttca ttagcatcca
    13921 cgacttctag cagttacacc aattggtcgc acaatgaaat taaaactttt gcacaagatg
    13981 gtaacatgga aatttttgat ttaatatatg caaccttaca tgacgttgca ccgctcatac
    14041 ctaaaattga atatgaaatg ataattaatt ctgcgcctga tgaaggaccc tattcttatg
    14101 acaacagtga ctattcttta ggctggagcg aaaactcaag atggtctcat ccttttaaga
    14161 tttcatctga tccgggtggc aagaatggta atgattacat gtggctttat aatttatatc
    14221 ggttggcttt tgctagcgat ggtgatgttg caagtgaagc ttataatgat aggcaaacta
    14281 atcctgttga attttattat tgcccggcaa tagtaaaaaa tacgtttaca aattcgcaac
    14341 aatggttaga agtaaacaat agtttttcaa taaacaggaa attcaaaaac tatctagatt
    14401 ttaagtgtgg cattaaagag tatacttcta aaaacactac cataaaaagc caatacacaa
    14461 tgcaaataaa aacagatttg gtttttccaa cattttctta tttcgatgtt gatgggggca
    14521 aaattgaagt tggggaacct acaaataatt tctatgacca aggaaatatt ataatgcatc
    14581 aaggtgattt atattttcat gccaacggac aatgtgagat ttttggaaac ggaaaaataa
    14641 tcgttgaagc tgcatcttta aatttaggtg gatcatcgaa aactatttta cattcagggg
    14701 ctgaaataat tattaagtca ggtggttcgt tggtttttgg tcaaaattca caaataattt
    14761 tggaggacgg tgcgaaaatt acagttgaaa atggtggtga gttacgttat tataatgatg
    14821 ttgatttgca attgaaaggc gataatgcag tattagaaat agcagggaaa ctagatattc
    14881 aagacaacgc cacattcacc ttcacctacc ccggagcaaa tagtggttat gtaaaattca
    14941 gtaatgttga aaccgatatt tataccccaa ccgaaaatat aaactacgga accaatagta
    15001 aaataaacat acatggacaa ggaaagaccg ataaggtgct tgaggtaaca caagaatcta
    15061 tttatacttc gggtgcagcc aatggcactt gtgccgagtt tagagtaact gatggcaagg
    15121 tagaaatgag taccaatgct cgcttaaatt tagactgtaa agtatttatc aataatgcaa
    15181 ctattgcaag ttcgtttgca ccgggcacca gtatagcacg aggcatagat gtatttggcc
    15241 agccaagtgt atcaatttca aattccgatt ttattggttt acaaaagggc ataaacgggc
    15301 tgttatttta tggcggcaaa tcgctttcgg tacccggttg cagttttatt caatgcgata
    15361 gaggattaaa cattgaaggt gcaacggttt atgtaaacgc atgcacattt atcgaaaatg
    15421 gtattggcgt aaaaattcaa aacataacaa aacccgcata cattcgtcac agtaatttta
    15481 cagtaaatga ttataatacg ggcatcagcg cttcggtagc aacaggcaca ccgctcaatg
    15541 tttggttctg caatattaat ggtggacctt atgaaatgaa cgaattaaca ggaataaata
    15601 ttgatggtgg taatttaaat ttaaaatgca ccaacataca ttacatgcga tatggtgtaa
    15661 aggccaacga caattcgttt gtaaacatga gtgccgaaaa tggatttggt tacaatgatt
    15721 tatcgaacaa cgaaacagca atagcatttc aaaaatgcga tggtgtaaat tttaaaaacg
    15781 ggcataataa tttttctttc aatccaaatt tgctcagcgg atactacaat cccaacaata
    15841 cttgggttac aaatagttgc agttattgca attttaatgg gctacctcgt atgagtggag
    15901 agtttacaca tccaccatgc tttaacaatg gtggaacatt aatctttgaa tgtgaccaaa
    15961 atcaatggaa tataaatgat gttataggcc ctgccatgtc cacacaaact taccaagcaa
    16021 caaacactta tgacaggtat gatgttggat atgtatgctc caataactct catgtggccg
    16081 atgtaatttt tgccgaccca aatccttcgg cagcgcaagc gtgtccaccg ggttcatcag
    16141 attgtgtaaa tcctccttgc ttggcgatat caaatattga tgtgagtaca ttgcgtgtaa
    16201 taaatacacc tacgtttcaa aatcaagaaa tggaagtggc tatcaataat gcaacggata
    16261 atataaatga aactgctgat ggtaaccgct ctacaatcat gctcttgaaa gaaatattag
    16321 actacaatct tactaaccct acgcaagaag aagtcagggt tttggattac tctatacgta
    16381 ccattcaaat ggcattggca aatttacaat tattaaaaac cgaggtgagc gatacaattg
    16441 aagtagaaag ttattttaca gcaacactcc aattgttgga taataaaatt gcacaggccg
    16501 tagccgaaaa taattattat catcatgtgt acctgatgtt cgacaaagca tgtgtgcaac
    16561 atttgcatgg cgataggatg gcagctattt ctacaataga acaaatacaa ttgtttgcaa
    16621 gcgcagaaga agtggattat ttagcctatt ggcattgtct ttatgacttg gaaaatcagg
    16681 caattacagg cgtgataaca aaggaagaat acaccaacac acttgaaatg tgcgccccac
    16741 cacaatattt acgggtgatg aatatttcgg agaatcaact agagcgtgaa gttaaacaca
    16801 atccttatca ttttaatgtg gacaacacaa actccatggc tcaattaaaa ttcaactcac
    16861 aaattgcaga aaacacaacc atatctgtat acgatttatc gggtaaaata gtttcgtcac
    16921 aaagctttga tacgcaaaag ggagaaaata atttcgtcat aaatttggca aacctgagca
    16981 aatctattta tgtagttcaa tttaaagtaa atgaaaatgt gtgtagggaa aaggctggat
    17041 tgtattaggc gctaaaagga aaagagtata tgtttttaaa aattcacatt gcaaagcctc
    17101 aaattatttt gaggctttgt tttttttaca ctctgtcaag atgctttcac taaccaaggt
    17161 tgttaccaaa tttgaaaggg cacaatgttt ttcgacacaa atacaaaaga aattatatcg
    17221 atattttatt atcttttcag ccacgcttgc ggatcaagtt tggtaaagcc tttccagatt
    17281 tggaaattta attctgcaac attggtttcg gtatcgcaat gtatagtgcc gattttttgt
    17341 ttggttgtaa gcttatcgcc tttgcgtacc aaaactcctt cgaggtttga gtatacggtt
    17401 aagaattcgc catggcgtac tattactgcc gcatagctgc cgggtatatt tatcactcca
    17461 gtaacttcgc ctgcaaaaat gcatcgtgcc gcagcacctt tcgagctttg aatatcgacc
    17521 ccatcgtttt tcactgttac gtttttcaat accggatgtt gatgttcgcc aaatgtagaa
    17581 accacctcac cgttttcaac aggccatggc agcacaccac ggttcgatgc aaagctgttc
    17641 gatagtttaa gttcttcggg tgtttggcta aaagcatttt tgctggtaat atcatccttc
    17701 ttcttacctc ctgcttcggc cttctttctt actgattcaa tttctttacg aatattttcg
    17761 gcaatggcat tttcaagttt tttcttggtg gcttcttttt ctgccagtcg tttgcgcaac
    17821 acactttctt gcttcgaaag gtttttcata aaaccttcct gagcgtgttt ttcttcggct
    17881 atgttttgtt tttgttgctc ctcggtagtt ttcaatttgc tttttatttg ccgttgattc
    17941 tgcaaaaatg tttgacggat tgtcaattcc ttttgaaccg agtcgatgcg aaaagcctgc
    18001 actttgcggg catcgctgta ctgctgaaaa tattttatcc gtttggttgc ctgcaaaaaa
    18061 tctttcgagg caaatatgta catcatcttt tgatatgagc tgcgattgcg ttgtgcaaaa
    18121 gttatcatgc gtgcatactc tgcttttagt tgcatcattt ccgattcaag atttgctatc
    18181 tcttgttcgg tttgttctat ttcgctgcca agatttccta tttgattatt gatggttcgt
    18241 ataagttgct cgcgtgtacg cagctgtttt gccaattgtg ccagttgcga ttttgtagcc
    18301 ttttggtttt tttcgttaag ctttagttga gcatttgttt cttctatttc gcgcagcaat
    18361 tcattttttt gtttttctaa atcgttacgt gttttgctta ctggttgtgc tatgcttttt
    18421 tgctgtatgc aaaaattgaa aagcaacaaa ataaaaacgc atttaaaaac atgcgaaaaa
    18481 ttaaacatta tataaggaaa taataattta cctgtgcggc attggctcat acgactgtgg
    18541 aatattaaac ggcatttcta caggcaaatt taccgtaact ttattgtact gtatgacgat
    18601 agataatttt ttatcagcac tcacttgtgt attaagcaac ataggaaata cgcctgcttc
    18661 tgttacttcg aatgagttat aatcggtggt aagggttttg tttagtttat catcggtaat
    18721 ttgtaattgg tctactttgt aatttgctgg gttcacgtag aagtcctgta taataggctt
    18781 gttggggtct ttgtcttcga gcgatcgttt cagcttacgt ttgttcaatg tgcttaaaat
    18841 atatcctttg tcttcaatat agaccgaatt gaatttgttt tcattgcgat attgaaagaa
    18901 attgccgatg ataatatttt gaatagtatt ataatccact ttaagtttta acaaatcgtt
    18961 gaggtattta aaatcggcct tggtatattt tttattcaac ctgtccatta caaaaatcga
    19021 atcggtagtg ataagaatgc gtgctacttc aatacctaaa atggcattta tcgaaatcca
    19081 gatagcactg tcgttttgaa ttctaatgtt ggcattaaat tcttttttat cgtttgtatc
    19141 tatcacggca acatcggcct tggcactcat ggtaacagcg ctgaaacgat atttgtacaa
    19201 cgaatcgata agtgatgatg atgaaaactt ctggtaattg atggtatcga atgcaatagg
    19261 aacggttacg gttttagttg ttacttttcg tgtggattta catgcggcaa aagcagcaaa
    19321 caaaaacaac aacaacaaaa cacgatgaaa tgtaaaaaca acattacgtg tcggaaaaga
    19381 aattacttta ttcaaaatat tttaatcgaa caattttttt tcgtaaatct tttgtttaat
    19441 cagttcgctg gcatcgcctg cctgttcggc cttttgccaa tattcaagcg ccttttgagg
    19501 gtcgttgagt ttaaatagca catcgccata atgctcaagt attgcaccat ttttgtcacc
    19561 accattttgc aatgcacttt ctaaaatagt ttttgcttcg gcatatttgc cttgcgcata
    19621 cagcacccat gcatatgtat cttcgtatgt agcaacatgc ggattaagct cggtaacttt
    19681 ctttgccatc tctgcagctt tgtcaagatt ttctttacgc aacgataaat aataagccca
    19741 gttatttagc acactgctat ttttaggatc gagtttcaaa accttctcat aatatttatc
    19801 gctctcggga taatttttta cattgtaata attatcgccc aggctggaat aaaaatcgag
    19861 caaaagtgga tcgcccagca caaccatttt gctaccggcc atcaacgatt taattgccgt
    19921 ttcgtaactt ttcaattgag cgtttgcaat tccattgtaa agaaatacta caggctgcgt
    19981 aggaaaaagt tccatagcct gattgcttgt tttgtccaac tctacccagt cggcaagttg
    20041 caaatcgcaa ttcataagtt gctgccatgt tgcaaaagtt ttagtgtcct tttttaaact
    20101 ggcgcgcaag tgtgtgcggg cttcattgta atttttgtcc tgaattaata tgtcggcata
    20161 tatagcatgt gccatgggat cgtcttcttc ggtatcaaca aaaattttgg aaagttcttt
    20221 tgcctgagct ttcattcctt cgtccacatt catcagcggc aagtacgata gcatgatttt
    20281 gtattttgtt tcggcaggta attttttgct acccatcgcc tttttcaatt cggcatacga
    20341 tttttctttt tcgccttttg cgcgatagtg ttgtgccagt gacaaagcaa tgcggggatc
    20401 atcggcatct atttttttca tgcgctgaat tacttcgtaa gctttgtctt cttcattatt
    20461 tatttgatac aactctacca gtaagccata gttttctaac gatggatcgc tggttattaa
    20521 tttttctaat tcttcggcag ctttatcgag cttgcccatc ttgatgtata tacgttgctt
    20581 ttcttttaca atatcggcat cgacaccaaa tcgcttttct attttatcga acactttgag
    20641 cgcagcttca ttatcattgt tttgcatgta tgattcaaca agcatgaaat agtaatttat
    20701 atcgccaggt ttttgcttta ccaatgtttc ataaacttca atggctggtt tggctttgtt
    20761 gtttatctgt aaaagttctg catactccat agcgtaccac tcgttggtat tgtcttcttc
    20821 gtatgcactg gcggcatatt caagcgcttc gtcattttta ttttgattgc gcagtaattt
    20881 tgcaatttca tacatcgatg ctgcatgatg cttatcgata cttaaacatt gcttaaaaac
    20941 atttatggct ttttcggcac tgccggtatt tttttcgcga tgtgcatcta caaacaatct
    21001 atcgagggtc atgcgcgact cgctgctaac attatccttt tttttctttt gtgcataaat
    21061 aacatttaca caaaacacat ttacaaaaac aagacaacta cataacgaaa taaaatacca
    21121 ctttgaattt accactttat acatgaaata tttttttata aaaaaactaa aaagcaaatg
    21181 aactaactat gattgtgtac tgtaatcacc catgctcaaa tcttgtacct tgccggtaaa
    21241 ttgacaaaaa tttccaatca ttgcattgtc aattaacgca tcattaactt tagtgtttgt
    21301 ttgtataata gaattgcgca ccacactgtt attcacttca catttatcgc caatagaaac
    21361 gtgcgggccc aacactgtat tgctcagttt tacatttgca ccaatataac acggtggtat
    21421 aataacgctg ttaatcagct cggccgattt gtcaataata ttattgggat attttatttc
    21481 gagcacacgt ttgttggtgt aaacagtggc gtccttattt ccacaatcga gccattctgt
    21541 tactttaccg ggcacaagtt ttttgccttt tactttcatg ttttccatgg cgttggtaag
    21601 ttgatattcg cccttatctt ttatatcgtt atcgataaga tattgcaact cgctgcgcaa
    21661 attttcgcca tccttaaaat aatatattcc aatgatagca agatccgaaa caaatgtttg
    21721 aggcttctct acaaagtcgg taatcacacc atcggcattt agttttacta caccaaactg
    21781 acgaggatct tcaatttgct ttacccatat ggtgccatcg ctattttcat caatgttggc
    21841 atcggtgcga aaaagagtat cggcaaaagc caccactact ttgcctttga gggcgctttg
    21901 tgcacatagt atagcatggg ctgttcccag aggttcttcc tgataatgaa tggtacccac
    21961 ggctcccaac gatgcagcaa tattttttaa attggcttcg gcttcttcac caaagtgccc
    22021 aactacatac gctatttcgt ctactttttc ggggcatact tttactatgt cttccattag
    22081 gtgttgcact atggctttgc cggcaagcgg tatcaatggt tttggaactg tgagtgtgtg
    22141 cgggcgcatg cgcttcccca ttcccgccat cggaacaata atcttcatat tttagttctt
    22201 agtttttagt tcttagtttt taacttttaa attaatcctt ttgaattaga tttaagtttt
    22261 ttgatttaat tttttcctgt actgccaaag cctccggcac cgcgttcggt ggcatcgagg
    22321 gtatgcactg cttttaattc tacttgagca taggttgcaa ttatcatctg tgcaattcgg
    22381 tcaccattgt ttattgtaaa tatttcgtgg ccatggttta tgagcattac ttttatttca
    22441 ccacgataat cggcatcgat ggttcccggg gcgttgagca cagtaacccc gtttttaaat
    22501 gccagtccgc tacgtgggcg cacctgtgct tcgtaacccg tgggcaatgc catgtataag
    22561 cctgtaggca ccaatgcacg ctcgcccggt tgcatgtgca tgggagcttc tatacttgct
    22621 cgcaaatcca taccggcact tgatggtgtt tcgtagccgg gcaatacatg ttgcgattta
    22681 ttgataatat gtacctgtgt cattttgctt ttaaaaaaaa cgtgcgaatt tagtttttta
    22741 atgtttacca aaatgctttt accgaaaatc gttggtgcga ataagttaaa gcacattttt
    22801 gggtaaccat actattttta caaacctaat ttattggttt aaagttttga gtccactccg
    22861 aaaagtaatt tttggcacaa aacacgaagg cacaaaattt ctctaaatcg tataaaaatc
    22921 attaataatg ttttgtggga tttagtgttt tggtgatttt gtggcatttt tcttttttag
    22981 acttttcgaa ggagagacaa gtttttgaaa tcgtattgcc tttaaattgc ataatggcta
    23041 ttgttcaaat tgtaatgccc taataatcaa ggtaagcatt gataaaacaa aaaaagacgg
    23101 cccttgcggg tcgccctttc cataaatttt aaagtagtgc tttaaaatta ttttttagta
    23161 acgcggcaag aaattctacg attttgtgca cgtccttctt ctgtatcgtt tgtagcaaca
    23221 ggaaactggt cgccatagcc ttcagctttt aagcgtgctg catcaacacc aagttttacc
    23281 agctctgcca tcacattatt ggcacgtgcg gcagatagtt tcaagtttgc atcagccttg
    23341 ccggtattgt ctgtataacc accgattttt aaatctacac cgggaaatgc ttttaatatt
    23401 tcaaccatat ttttcaattg ctcttgtgac tcaggcttca agcttgcact tcctgtttcg
    23461 aacaacaaac ggtcgaaatt gaaccatgta gttttgtcaa caggcttgtt tgcatcttca
    23521 ataaatgtaa tcaattgatt ttcgattccg gtttcaggga tgtttaattc aataccggta
    23581 gaaagtttgc gcgtgaaaaa tttaccaaga tttgcccata atccgttggc ttcatcggct
    23641 acattagctg ctgcgctatc agctgcaact gcaactgagg ttgcagcttc ttcaccatgc
    23701 gcggcagctt cggtggttgc ttcggtacca tgttcttcgg ctttgccaca tcctttccaa
    23761 aaatataaag ctatcattgc tataaatgca gcaagtaacc agggcgaaaa tttagctagg
    23821 ccgctcgatt tttcctgtgt gttttgatat gcagaacttg cagccgaagt tacagttgaa
    23881 gcagcattag atgcaccgga aaacaatttt ccgccaaggt cggcaatgct gccaagacct
    23941 aacattgaag ttaaaccaga aggaatgagg ccgcttataa aacttttttg actgccaagt
    24001 aatgaagtaa aacctctaag gtctaatcca ttagatgcta cttctttacc aaacaaattc
    24061 atcacaaccg gtgccaacat tgctaacaaa gatgatgatg attttgatga aatgccactg
    24121 gttgatgaca ataaatcggc aactccagat gatttgctgc caaacaatgt atccattagg
    24181 cctgcacctt tcgatactaa attgttcgtt tcgtcaccac caccaaacat accactaagg
    24241 ttatttagca tgctgccgtt attgttgccc gagttgagca ggttaaaaat cattgatgct
    24301 ccattgctat cggaggcttt gttcattaat cctgcaacca aagttggtat agcagtggaa
    24361 acagcttttg ctgttgatga ttcgttctcg cctaagaaag aacttgcttt gctgattact
    24421 tcgtttgtca agtaacccga tgccatgtca atgatgttga atgccatttt ttaaattgat
    24481 ttttagaggt ttatttaaat ttaaattctg agccgccaat tgctaaactc gtgccaaatt
    24541 tattttgttt acttaaactt catatacaat aatggagggc gcactcaaaa atgtttgatt
    24601 tacaattgaa aaaatgccat caataatatt gagaaaaggg ctctatttcg aactcaaact
    24661 aataataggt acaatcattt cttcgagtga tacaccccca tgttggaatg tatcgcgata
    24721 gtatgttaca taataattgt agttgttggg gtaggcaaaa aaatcatcgc tctttgcaaa
    24781 cacaaacgaa gagctaacat gttggcgagg tagcattgca tcggcagggt tttttatttc
    24841 gaatacttct tttttatcgt acgacaaact cttgccattt ttgtaacgta agtttgagtt
    24901 ggtgtttttg tcgcccacaa tttttaccgc ttgctttact cgaattgtgc cgtggtcggt
    24961 ggtgatgata agtttacatg gcttttcgga tacacgcttc aaagcttcga acaaaggcga
    25021 attcataaac caactgagtg taatgctgcg ataagatttt tcgttttcgg caagttcgcg
    25081 aatcatttcc atttcggtgc gtgcatgaga aagcatatct acaaaattat acactattac
    25141 attcaattta ttatcggcca tacggtgtat gttttctaca agttccttac ccgattgatg
    25201 gttggtaatt tttgtatacg aaaatttata attctttcgg ttgcgttgca attggtcggc
    25261 aagaaaatcg ggttcgtaca aattttttcc ctcatcttct tcgtcattga accacatttt
    25321 aggaaaacgt ttttcgattt cggatggcat cattcctgca aatattgcat tgcgcgcata
    25381 ctgtgttgct gttggtaaaa tagcgcaata cgtatcttct tctgtcactc taaaatattc
    25441 gcttatcacc ggttgaatta ttttccattg atcccaacgg aggttatcta tcaacacaaa
    25501 ataaattggt tcttctacgg catcggccat tgggaaaact ttgttcttaa ataactgatg
    25561 cgatagcgat gggccctggt cgggtgtttt gagccacgat acataattat tttctacaaa
    25621 tttgctccac attaaattgg cttcgtcttt ttggcttacc aatatttcgt acatactttg
    25681 gtcggggtta cgctctaatt ccaattccca atacaccaat tttttataca catcaaccca
    25741 atcattgtag ttgaggcggt cggtaatgcg cataccaatt tgcctgaatt cctgtaagta
    25801 tagcgaattg cttttttcag aaataagccg tttgttatcg agattttttt tcaacgaaag
    25861 taaaatctga tttgggttta caggcttaat caaatagtcg gcaattttat ttccaatggc
    25921 atcctccatt atatgttctt cttcgctctt cgtaatcatt atcaccggta aatgcgattt
    25981 cagatttttt atttgcgcca aagtttccaa tcccgaaagg ccgggcatgt tttcgtccaa
    26041 taaaataata tcgaaatcct ggttgcgcac tttttcaatt gcatcgtcac cattatttac
    26101 ggtaaggaca tcatatccct tttcgcgtaa aaaaataaca tgtggcagca ataaatc
//
LOCUS       DAORQA010000019        22086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_592_length_22086_cov_28.132087, whole genome shotgun sequence.
ACCESSION   DAORQA010000019 DAORQA010000000
VERSION     DAORQA010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22086
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_592_length_22086_cov_28.132087"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(761..1057)
                     /locus_tag="PK736_02915"
     CDS             complement(761..1057)
                     /locus_tag="PK736_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRC32376.1"
                     /translation="MERGGYIYIITNQNNTTLYVGVTSKLKQRIWEHKNKVYPNSFSA
                     KYNLNKLVYYEGHESIDEAIAREKQLKAGPRKKKEKLILDSNPHWNDLYDYVEE"
     gene            complement(1184..1726)
                     /gene="ribH"
                     /locus_tag="PK736_02920"
     CDS             complement(1184..1726)
                     /gene="ribH"
                     /locus_tag="PK736_02920"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRC32377.1"
                     /translation="MSSANKNLSEYDASQLPDGSAMRFGIVTSKWNTEITGALRTACL
                     QTLQSANVNSKNIVEMEVPGSFELTTGASWLLQSGEVDCVICLGCVIQGETRHFDFIC
                     NAISNGLTKLSINYGKAVIFGVLTPNNMQQAQDRAGGKHGNKGVEAATTALQMVQLYN
                     TEIQRRRDNFQNYLDHGKNM"
     gene            complement(1864..2994)
                     /locus_tag="PK736_02925"
     CDS             complement(1864..2994)
                     /locus_tag="PK736_02925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC32378.1"
                     /translation="MEIIGYAVAALVIFYLVWIFKLASALRNANIDLPSAIPDISVSI
                     VVAMRNEQEHILNLLNALRAQKNPPLQTELILIDDFSTDNTLQQIESWKVYNSDFNIR
                     VIAMNDFPNAGKGKKDAILLGINSAMHDYIITTDADCTMQANWLNTMCNYFISARPAL
                     LAGPVMLHADILFGKLQQMEMILLQALTCATFKLNKPVMCNAANLMFRKMDYLSLNKN
                     ALNAKTASGDDIFLMLALKQKGLKMDYCCHQQCVVKTNAINDFSDFVHQRIRWASKIK
                     LINDKDILTAGAVVMIANLLLLFFFGMAIACITPWYYFLFLFTLKLLCDVVLINFVSQ
                     KLEMKQVSYTVIIIFQIIFPFYNILIAALSIFGKYKWKGRGY"
     gene            complement(2985..3938)
                     /locus_tag="PK736_02930"
     CDS             complement(2985..3938)
                     /locus_tag="PK736_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32379.1"
                     /translation="MTSKTVSALKLLFATALCFIIVRETILHFNTIELSNLALSKLFS
                     PQFFIFLVSAVTLVFINWGLEIYKWQVLVNSVVAIKFYDAVKAVMSGVSISLFMPNRT
                     GEFAGRILFVPADKRIETTVVTFYGSLIQLVITLAFGLVSYFVLQHYHHFSIPFVNAL
                     FLQINLPVLLIIVVGLILISFFFRKKLLVLFHSVLLKLKYIKISPALHAKVWFLSALR
                     FIVFTMQFVLLMLAFGVHGNLISFFCGASFILFATTILPSISVLEIPLRGGISFLVMN
                     SFSTYASGASVASLLLWCINLGIPAVFGAIILMFFKFDKWK"
     gene            4135..5322
                     /gene="kbl"
                     /locus_tag="PK736_02935"
     CDS             4135..5322
                     /gene="kbl"
                     /locus_tag="PK736_02935"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRC32380.1"
                     /translation="MYETLKPILEKEIADIQAAGLYKRERIISSPQGAVITVNGKEVL
                     NFCANNYLGLSSHPDVLKAAHHALDTHGYGLSSVRFICGTQDIHKTLEAKLATFLGTD
                     DTILYAAAFDANGGIFEPLLNEQDAIISDELNHASIIDGVRLCKADRLRYSHNNMEDL
                     EAKLQSTQNKRHRMIVTDGVFSMDGTIAQLDKICNLADKYKALVMIDECHASGFVGKT
                     GRGTHEYRNVTGRIDIITGTLGKALGGAMGGFTSARKEIVEILRQRSRPYLFSNSLAP
                     SIVGASIAVIDMISQSTTLRDKLWDNTKYFREQMTAAGFDIKPGEHPIVPVMLYEAPL
                     AQKFADALLQEGIYVIGFFYPVVAKGNARIRVQLSAGHSLEQVQKAIDAFTKVGKTLN
                     VIK"
     gene            5380..7572
                     /locus_tag="PK736_02940"
     CDS             5380..7572
                     /locus_tag="PK736_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRC32381.1"
                     /translation="MNKFVIAIVIAIISTINLHSQSFDNYVNITSEGTLPIDVIRLSS
                     EKYLADKTQIDKKNTTTLERKAQDVFYQSSNYQLNELLHSGLILFDNELTKYVNKVAD
                     HAFVAYPELRGKLKIYVARSTAVNAFTFDNGTIIVNAGLLANLDNEAQLAFILCHELT
                     HYKDKHVIEGFVENVKIEKKASITGSRNNETRLLQKSKFSKQQEFAADAQGLEMFYKT
                     QYDKKVPLQCFEILRDYITPFKQTREFTTKFFNPYTSVASDTIKLDSLYFFDFDKGDD
                     DSDDDGRFATHPHIDARIMEIHSSLEEMSASTKPEDNKLFIETESDFNRIRNVARFEM
                     CKLYIQNREFSESFYATYNLQKEFPDNKYLSRCMIRSLYGLAKYKNSDQISEVVKKPK
                     KTTQPLQPVCNFFREVDAEELNFTALAMAYNQYNKDTTDEEMKSIVRQLIYQYVKYHD
                     KEGVFLRKATNKFEKFSSKDSVYFKRYSYNTLQKYFDDKWFTKRYQIAYDKVNEPDEV
                     LTASQKKLKAKKRNYEDKHGLSLGKDSIMIIDPTYLQFDLRNRSNPLHLQASESNLLE
                     YYNTIRDISKKVDLKTQIITPYELNAQDIEKFNTLMTLKDVMREYEKNDDVFLIPFDY
                     SAIEGIRKKYNINYIADMGVLSARLKKPSEKYFTSCVAMLCYPLIPFLVYGLAKPEYE
                     TYNFILLYDLENFEQKTILHRMNNKDKISVTSSALYFNFLQIKRESKY"
     gene            7584..8303
                     /locus_tag="PK736_02945"
     CDS             7584..8303
                     /locus_tag="PK736_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32382.1"
                     /translation="MKKNNIFLFAFALLMLSQNVHAQSPGYLGKRVAISAETNFCPLV
                     GYLINNDEIGDFNWRYAGGIDYVVSNYQTIGLKVETMNDDFKYKSYVTSDDNYSSSYY
                     YYDQTYKATLNTTAIIFSLKTFNLNKGAIAPLGNYAALELGYLFGNFDVNDFNVSSTT
                     KQLEAFLFTYGMSSRVIYKSRVFVDIGFKAGLAINGNAKYYLSEVSNIKQYKALEKRS
                     ATGTMLTNYLFSIQMAIGFIP"
     gene            complement(8340..10301)
                     /locus_tag="PK736_02950"
     CDS             complement(8340..10301)
                     /locus_tag="PK736_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019495278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRC32383.1"
                     /translation="MKKIIFPKVLFTLCIVANISTGIFAQATKREVGNLVMEGIPEIP
                     ADIREKMNQYQNTRSASFSDWSADGKSILLSTRFGDVNQLHIIDHAGGARKQITFFKE
                     PVNGGVFIPLPRTQRFMYTKDVGGNEFSQLFVFDLTNGSYKMISDGGRSQNSSPSFSN
                     SGHQFCVVSTRRNGKDYDLYLYDTQTPDKGKIILQNGGSWSVADWSPDDKKMVVKLYI
                     SANRSEIYLLDIATGLTEQINKSKEEISYGSVVFSQDGKGLFIVNDEGSEFMTLKYYD
                     IATKKFTSITQSIPWNVERVAENKNRSKIVFSVNNNGNSNLYEMNALTKEYIPINDLP
                     VGVIGGIKFNPVNDVIAMTINSPQSPGDIYTYDLGAHILKQWTFSEVGGLNPNTFVTP
                     TLISYKTFDKPPGAMQQIPAWYYKPKKADAKLPVVILIHGGPEGQSLPTFNAYINYLT
                     NELGIAVIAPNVRGSEGYGKTYLKSDNGFNRENSVKDIGALLDWIAQQPELDASRIAV
                     YGGSYGGYMVLASLTNYNDKLKCGIDVVGISNFVTFLTNTEDYRKDLRRVEYGDEREP
                     KMKEFLTKISPASNASKITKPLFIIQGLNDPRVPASEAEQMKVKIAEKGGEVWYLVAK
                     DEGHGFRKKVNIDFQQWAMILFLQEKLLK"
     gene            complement(10317..13664)
                     /locus_tag="PK736_02955"
     CDS             complement(10317..13664)
                     /locus_tag="PK736_02955"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32384.1"
                     /translation="MKKLFLFLFLTTQFCNAQSWVNMMHDRNANFYDTQKEFYKYYNN
                     EVRKQNVWHKKLTRLFSKETEQEIPGFEVFKRWEYFTAPRVYPTGRMFDGDQVWNEFQ
                     NYCKKNGIDQTNVAGNWTTLGPSSWQSTSYNPGLGRVNVVYVDPNNAASIFIGAPAGG
                     LWHSPDTGNTWASNTDQLPTLGVSAIAVPLGNSNTIYMGTGDKDAGDIYGVGILKSTD
                     GGITWNQTGLTWTLSQAYNVSKILIFPGDTSSLIASTSNGIYKSNNAGATWTKVYSGR
                     YRDVEFMPGDTNIVFASGTSFVRSTDGGNTWTVITAGLPLSADVNHLQIGVTPADANY
                     VYIVAGAAASSGFYGLYKSTNGGLTFSQQSSTPNIFTYSETGNGTGGQSWYDMTLCVS
                     PSNKNEIYAGGINIWKSTDGGINWFIRTHWFYDAGANYPYVHADQHYMDFYGDKLYVG
                     CDGGVFTSDDYGNTWTDITAGATIAQFYRLGASATDPTKIMAGAQDNGCNYYNNGQWT
                     HVLGADGMETALDPNDPNILYACGQGGWLNRSDDGGQSMNTISTNQMGNGNWITPYML
                     DPNNSNTIYAGYAEMWKSTDNGDNWAQISNFALASNVSAFNIAPSNSNVIYVIVNNKL
                     MYTDDGGNNWIDITSNLPNGSASINSIAIHPSNADRVWVCFSGSSPGNKIFYSGDAGT
                     TWTNISGNLLNVSTNSIVYQTGTNDALYVGTDIGVFYTDSTLSNWQPFWNGLPNVSIS
                     ELEINYVANKIRAASYGRGLWESDLYVPLQASPTANFVSDKISICPGESIQFSDASIN
                     AAPGWTWSFPGGTPSTSTLASPKITYATIGVYDVQLIVNNAFGTDTLLKTNYVNVTIP
                     VAQAVPFTEGFENNFPVTDWKILNPDGQATWSQSSSVGGYGLSGSSAVINNYSYNQPG
                     QSDVILTAPYNLTMLANPALFFDVAYAIFPGYADTLNVFYTTDCGVTRTSIFKKGGSD
                     LATAPSTQSQYAPANNEWITYQLSLAAVANNTAVQFGFENINGYGNRLYLDNININQS
                     TGVAENISSTLQLYPNPSSGFFIINDLKNELKIIEVFNQHGQLLMSMKNALPQNRIDL
                     SAFENGLYIVKINTSTEAISHKITLLK"
     gene            13907..16153
                     /locus_tag="PK736_02960"
     CDS             13907..16153
                     /locus_tag="PK736_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32385.1"
                     /translation="MRNFYFLIVCLFAIQISSAQIIGSDTTCAGYIYQYDFTYPAAAS
                     YTWTVPNGWYILSGQGTGSVQLYCNVSAGNICVDALDSSLNIINNDCKPVTLGGGGSG
                     WYVDLTPAGPCICSPNFLVIVPNGTGGNCGGCGNGTPSASSYYAAYDNPWPTGNYLGP
                     IGPGGVTIGGPFGGTYYVYYIDTILGFSNAFVVDGGACGGSVNNAITVFPCFPPMVPL
                     LQSPASICPGDTFTVYVDPSLLIFWASSLWNPSGNNISVINTYTDSIQCILLYNQPGS
                     GIQAFFSDIYQCPYGAIIALNIATNCSPNDPCLIAAYPLDGNAIDTTINGNNATFNNT
                     QLATNRFNIANTAAQFNGINNRIDLPDDFDFPQRTWAFWFYADTITTNYHEIFDDDNV
                     YIQNAQTEVFLHDDSTGVKKLYAAIGLNQYIIPVNEKQWYHLALVRDTGETRFYLNGC
                     LMQIGTNMANDHSFDGAISVSLGCNRFHNGNYFKGKLDDLFIYQCALTDSEIAMVADT
                     NCFVATPVAALQSSDTIWCDKKCIDFIDLSQFNPTIWQWTFAGASPPTSTDQNPTGIC
                     YNNYGTFDVTLVACNNGGCDTVTYLNFITEYQLPPQPTISFVSDTLFCSQANSYQWFI
                     IPNPIPGAVLQYYYPMVNGSYYVVISDSNGCGNASNTFIISGTIENNSNYFQNFYCDE
                     DGLCTIENLLQNGKVALSIFDFDGRKLNEKIISAQTETVDLKFLPKGIYIVQLMSEKK
                     LYNFKIVR"
     gene            complement(16812..18488)
                     /locus_tag="PK736_02965"
     CDS             complement(16812..18488)
                     /locus_tag="PK736_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32386.1"
                     /translation="MKTKTCLLICFCFIVYINSFAQTFISGFISTNTTWSVAGNPYIV
                     NGNALLTHGNTLTIEPGVIVKFETDKALQIDGELIAIGTFEDQITFTSNQANPQKGDW
                     AKIHFADTCIDAVFDSQGKYQSGSIMKYCNVLYAGGIGYGAIHMERSAPYLSYCRIIS
                     SFTNGIFSFESTYIIDSSLIKGCENFGIMNTYSYYLTGNIKIQTDTFEMNNGAIYFSG
                     NYTTYDRIVSNNVFRNNHGPNHLIFFDGQNFYFNNNLFVNNDFSGYGASLVYCPSSSE
                     AYIFCNIFLNNLFDVGSCIACPLGGEIHHNLFDGNIGINEATCLYIVTTYPTIYIYQN
                     ILNSNSNSLGSICKLIPPLAYPSVSMHVERNDFKNNIAPNTIFIDNAWQQTDPANYSL
                     YMKHNNFLNPENIEIYNNVQYGNQDFYLDSNYWGSTNPQHCDSVIYDYFDFANQSVVY
                     YLPMLSAAVVVDTMCSSIPNSIADLNLSTGKFLLFPNPANDLINVIIPIVNSQVELNI
                     YSTTMQLVKTINANSGGETTITVSDLPPGIYFIDAQADNQHWRQKLIINR"
     gene            18790..20802
                     /locus_tag="PK736_02970"
     CDS             18790..20802
                     /locus_tag="PK736_02970"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRC32387.1"
                     /translation="MMKGIGLLMMLIGMIGTSFAQLRNLNVSPSNADKVQKVEIQKVQ
                     NAHYCYSHEYMLQQMSQNPDMVKEQEKLEKETERLSRLTNENENQKQAVITIPVVFHV
                     LYNSTYENISDQQIQSQIDQWNRDFNRANWDSVYIPSVFKSKYTNMNIKFCLANRTPG
                     GAPSKGIIRKSTTKTTWTTGNAAIKRSAQGGDDAWNRDNYLNVWVVGALSGNVLAYAT
                     FPGGPAADDGIVSWNECLGTTGTVGYPYTLGKVLTHEVGHWLNLRHIWGDDGGACTGS
                     DQVGDTPNAWDANYGDPTFPHVTCSNGPNGDMYMNHMDYVNDQTKIMFTTGQKTRATA
                     ALNTTRVAIQTSNGCVAPNANAFSVPAGILARGVNENSASIWWNTVAGAASFELQYRK
                     NGTTAWTTKTAAQINYRISGLTVATKYDYRLRAIYANGNSAYSAIKTFTTASNSACNG
                     DPFEFNDTYHTPCPINSGKNYYAKLSATDMYDWYIVKSDASKPYIRITLSNLQQDYDL
                     QANLASGTFLASSTNSGTTNDVVNLAGASGEYFLVNVNIGANLVSTACYKLIVEYKAT
                     PFLANDDDIRLQEPSNEAIVKVDMMPNPASNEAHLNILTNENIDIAISVIDNLGKVVY
                     ANNTVAIEGENLIELPVNEFTNGLYQVIVSGNNFTEMKKLIVNKKN"
     gene            complement(20997..21923)
                     /locus_tag="PK736_02975"
     CDS             complement(20997..21923)
                     /locus_tag="PK736_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRC32388.1"
                     /translation="MSKKPHNKSSHIIQSLVVNLLIAISKSVAAFMTHSGAMLAEAIH
                     SFSDCANQILLLVGVRQSQKAATARHPLGQGRAIYFWSFMVAMLLFSIGGMFSIYEGI
                     HKFNMPEPIQHVSWGIGILFFSLLLESYAMYSNIVEMNVRRGKLSFMKYLRATKDSDL
                     VVVFGENSAAVFGLIVALVAMLASYYTGDSRYDAAGSFCIGVILICVAIFLSVEVKSL
                     LIGESASDEILQSVNDILQKHAEVIKLINCITIQQGPGEVMMCMKIKCAHNFTALELS
                     KLINKLEHEIRSGSPEVKWLYVEPDAQEWKEK"
BASE COUNT         7072 a         4276 c         4048 g         6690 t
ORIGIN      
        1 gcaaagcgaa gcaatctctt tttcctaccg tcattgcgag cgaagcgaag caatctcggc
       61 cgcgaaatgt ctttgcgatg ttgagattgc gtaagtcgtt cctccttcgc aatgacggaa
      121 agaaatgaga gtcatgtcga gcaaagcgaa gcaatctctt tttcctcccg tcattgcgag
      181 cgaagcgaag caatctcggc cgcgaaatgt ctttgcgatg ttgagattgc gtcagtcgtt
      241 cctccttcgc aatgacggaa agaaatgaga gtcatgtcga gcaaagcgaa gcaatctctt
      301 tttcctcccg tcattgcgag cgaagcgaag caatcccagc cgcgaaattt cgttgcgatg
      361 ttgagattgc tttagtcgtt cctccttcgc aatgacggaa agaaatgaga gtcatgtcaa
      421 gcaaagcgaa gcaatccctt tttcctccgg tcattgcgag cgaagcgaag caatcccctt
      481 tttcctcccg tcattgcgag cgaagcgaag gtatcccagc cgcgaaatgt ctttgcgatg
      541 ttgagattgc gtcagtcgtt cctccttcgc aatgacggaa agaaatgaga gtcatgtcga
      601 gcaaagcgaa gcaatctctt tttcctaccg tcattgcgag cgaagcgaag caatcccagc
      661 cgcgaaattt cgttgcgatg ttgagattgc gtcagtcgtt cctccttcgc aatgacggaa
      721 ggagaaaaat tagtactcaa tcaaaagtta atttagaaat ctattcttca acataatcat
      781 acaaatcgtt ccaatgagga ttcgaatcaa gaattaattt ctctttcttt tttctagggc
      841 cagcctttaa ttgtttttct ctcgctatag cttcatcaat actttcatgc ccttcgtaat
      901 aaacaagctt attcaaattg tattttgctg aaaagctatt tggataaact ttgtttttgt
      961 gttcccatat tctttgtttt aactttgagg ttacaccaac atatagagtt gtgttatttt
     1021 gattagttat aatataaata tatccgcctc tttccatgaa tatttatttt agcataaagt
     1081 ctgatattta atttttgcac tctcccacaa aatgtctttg cgatgcagag attgcttcag
     1141 tcgttcctcc tttgtaataa tgagaggaga gttaatcatc catctacata ttttttccat
     1201 gatcaagata attttgaaaa ttatctctcc gcctttgtat ttcagtatta tataattgca
     1261 ccatttgcaa agctgtagtg gccgcctcta cacctttgtt tccatgtttg cctcctgcac
     1321 ggtcctgtgc ttgttgcata ttgttgggtg taagcacacc aaagatcacc gccttaccat
     1381 agttgatact tagctttgta aggccatttg aaatggcatt acaaataaaa tcgaagtggc
     1441 gcgtttcgcc ttgtattaca cagccaaggc agattacaca atctacttcg ccactctgta
     1501 gcagccaact ggcacctgta gtgagttcga aactgccggg cacttccatc tctacaatat
     1561 ttttggaatt aacattggca gattgtaaag tctgcaaaca agccgtgcgc aaggctccgg
     1621 ttatttctgt attccatttc gatgttacaa taccaaatcg catagcactg ccatcgggta
     1681 attgtgaagc atcatattcc gataaatttt tatttgcact actcatactt aaatattttt
     1741 tgtaacaaca aaacaacgat ttttttttat gcctttatta ccaaaacatt tcaccaacca
     1801 aaatcatccg tttcatttgt taaatacatt ttactttcaa tcgtttcttg caataaaaca
     1861 ttttcaatat cccctaccct tccatttata ttttccaaaa atagacagtg ctgcaataag
     1921 tatattgtag aaagggaaaa tgatttgaaa tataataatg acagtgtaac taacctgctt
     1981 catttccaat ttctgcgaaa caaaattaat caacacaaca tcgcataata atttaagtgt
     2041 aaacaaaaac agaaagtagt accagggagt aatgcatgct attgccattc cgaaaaaaaa
     2101 taacagaagt aaattagcaa tcatcacaac agcaccagcc gtcaaaatat ctttgtcatt
     2161 aatcaattta attttcgatg cccaccgaat acgttgatgc acaaaatcag aaaaatcatt
     2221 tatcgcattt gtttttacaa cgcattgttg atggcaacaa taatccattt tcaatccttt
     2281 ttgttttaaa gcaagcatta aaaaaatatc atcgccactt gccgtttttg cattgagtgc
     2341 atttttattg agcgataaat aatccatttt gcgaaacatc aaattcgctg cattgcacat
     2401 tacgggctta ttcaatttaa aagttgcaca agtaagcgct tgcagcaata tcatttccat
     2461 ctgctgcagt ttaccaaaca atatatctgc atgcaacatt acaggaccgg ctaacaatgc
     2521 aggtcttgca gatataaaat aattacacat tgtattcagc cagtttgcct gcatggtaca
     2581 atcggcatcg gtggtgataa tataatcgtg cattgctgaa ttaataccca gaagaatcgc
     2641 atcttttttt cctttccctg cattcggaaa atcgttcatt gcaattactc ttatattaaa
     2701 atcgctgttg tatactttcc aactttcaat ttgttgtagt gtattgtcgg tggagaaatc
     2761 atcaattaaa atcaattccg tttgtagcgg aggatttttt tgagcacgaa gtgcattcaa
     2821 taaatttaaa atatgctctt gctcgtttcg catggcaaca actatcgaaa cagaaatatc
     2881 cggaattgca gatggtaaat caatatttgc attacgcaaa gctgaagcaa gtttgaaaat
     2941 ccaaaccaaa tagaaaataa ctaaagcagc tacggcatat ccaattattt ccatttatcg
     3001 aacttgaaaa acattaatat gatagcacca aatacggcag gtattccaag attgatacac
     3061 cacaacagta aagatgcaac gctggcacct gaggcatagg tgctaaaaga attcattacc
     3121 aggaaactta taccgccacg caaaggaatc tccaaaacag atatggaagg caatatggtt
     3181 gtggcaaata atatgaatga tgccccacaa aaaaaggaaa tcaaatttcc gtgtacgcca
     3241 aaggcaagca tcaacagcac aaattgcatt gtaaatacta tgaatcttaa agcagaaaga
     3301 aaccaaactt tggcatgaag tgcaggagaa atttttatgt attttaattt caacaaaaca
     3361 gaatgaaaca agaccaataa tttttttcta aagaagaaag aaattaaaat caaaccaacc
     3421 acaataatta aaagcacagg caaattgatt tgcaaaaaaa gtgcattaac aaatggtata
     3481 gaaaaatgat gatagtgctg caaaacaaaa tacgaaacta aaccaaatgc caatgttatc
     3541 accaattgaa tcaagctgcc ataaaaagta accactgttg tttctatgcg tttgtcggca
     3601 ggcacaaata atatacgtcc ggcaaattca cccgtgcggt ttggcataaa taaactaata
     3661 gaaacaccac tcataactgc tttaacggca tcataaaatt ttatggcaac aacagaattt
     3721 acaagcactt gccatttata aatttcgaga ccccaattaa taaacaccag agtaacggca
     3781 cttaccaaaa aaataaaaaa ctgcggagaa aataatttgg ataaagcaag gtttgacaat
     3841 tcaattgtgt tgaaatgaag aatagtttcg cgcactataa taaagcacaa agctgttgca
     3901 aacaataatt ttaaggccga tacggtttta cttgtcaacg cagttctcca attaaaatgt
     3961 aaacatattt tttatttgca ttaattgaac caataaattt caataacgaa aaaattcaac
     4021 ttgcatagga tggcaaagta tgaaactgta agtgcttctt ttgggaaaat aattttagta
     4081 ccatatcaat atttattttc catatttgta aaccaataat ttaatttcaa aaaaatgtac
     4141 gaaacactga aacctatact ggaaaaagaa attgctgata ttcaggcggc aggattatat
     4201 aagcgtgagc gaattatttc atcgccacaa ggtgctgtta taacggtaaa tgggaaagag
     4261 gttttaaact tctgcgccaa caattatctg ggattatcat cgcatccaga tgtgttgaag
     4321 gcggcacacc atgcactcga tacccatgga tacggacttt catcggtgcg gtttatttgt
     4381 ggcacacagg atattcacaa aacactcgaa gcaaaacttg ctactttttt gggtaccgat
     4441 gatactatat tgtatgctgc agctttcgat gccaacggag gcattttcga accattgttg
     4501 aatgagcaag atgcaatcat cagcgatgaa ttgaatcatg cttcgataat tgatggcgtg
     4561 cggttgtgta aggccgaccg tctacgttat tcacataata acatggaaga cctcgaagcg
     4621 aaattacaat cgacccaaaa taaacgccac cgtatgattg ttaccgatgg agtattttcg
     4681 atggatggca ctattgcaca actcgataaa atatgcaacc tggccgataa atacaaagcc
     4741 cttgtgatga ttgatgaatg tcatgcttcg gggtttgtgg gcaaaaccgg acgcggcaca
     4801 cacgaatata gaaatgtgac tggccgcata gatattatta ccggtacgct tggcaaagct
     4861 ttgggcggtg ctatgggcgg tttcacatcg gcacgaaaag aaattgtaga aattttacga
     4921 cagcgttctc gtccatattt atttagcaac tcgctggcac caagcattgt aggggcaagc
     4981 attgccgtta tagatatgat atcgcaaagc acaacgctgc gcgataaatt gtgggacaac
     5041 acaaaatatt ttcgtgagca aatgacagcg gccggcttcg acataaaacc gggtgaacat
     5101 cctattgtac cggtaatgct atacgaagca cctttggcac aaaagtttgc cgatgcactc
     5161 ttgcaagaag gtatttacgt tataggtttc ttttatccag tagtggcaaa aggcaatgca
     5221 cgtatacggg tacagttatc ggcaggccac agtctcgaac aagttcaaaa agccattgat
     5281 gcttttacta aggtaggcaa aactttaaat gtgataaagt aaatattact ttatcgcttc
     5341 atgaaaacaa aaacaaaact aatcagcaaa aaatcaaata tgaataaatt cgttattgca
     5401 attgttatag cgattatttc gactattaat ttacattccc aatcgttcga taattatgta
     5461 aacataacca gcgaaggcac attgccaatt gatgtgataa gactttcgtc cgaaaaatat
     5521 ttggccgaca aaactcaaat tgacaaaaaa aataccacca cacttgagcg caaagcacag
     5581 gatgtttttt atcaaagcag taattatcaa ttaaacgaat tgttgcatag cggtttaata
     5641 ttattcgata acgaacttac caagtatgta aataaggtgg ccgaccatgc ttttgttgct
     5701 tatcccgaac tgcggggtaa attaaaaata tatgttgccc gcagcaccgc tgttaatgcc
     5761 tttacattcg ataacggaac catcattgtg aatgctggcc tgcttgcaaa cctcgacaac
     5821 gaagcgcaac tggcgtttat tctatgtcat gaacttacac attacaaaga caaacatgta
     5881 atagaaggat ttgtggagaa tgtaaagatt gagaaaaaag cgagcattac cggaagcaga
     5941 aataatgaga cacgattgct tcaaaaatcg aaattttcga agcaacagga atttgctgcg
     6001 gatgctcaag ggttagaaat gttttacaaa acacaatacg ataaaaaagt gcctttgcag
     6061 tgtttcgaaa tactgcgcga ttatattacg ccattcaaac aaacacgaga atttacgaca
     6121 aaatttttta atccttatac atccgtagca agcgatacta taaaactgga ttcgctgtac
     6181 tttttcgatt ttgataaagg cgatgacgat agtgatgatg atggaaggtt tgcaacacat
     6241 ccacatatag atgcacgtat aatggaaata cactcgagcc tcgaagaaat gagtgcaagc
     6301 acaaaacccg aagacaacaa attatttatt gagactgaat cggattttaa cagaatacgc
     6361 aatgtggcgc gattcgaaat gtgtaaattg tatatccaaa atcgtgaatt ttcggaatcg
     6421 ttttatgcta cctacaattt acaaaaagaa tttcccgaca ataaatattt gtcgcgttgt
     6481 atgattcgca gtttatatgg tttagcaaaa tataaaaatt ctgaccagat aagtgaagtt
     6541 gtaaagaagc caaaaaaaac cactcagcca ttgcaacctg tatgtaattt ttttcgcgaa
     6601 gtagatgccg aggaacttaa ttttacagca ctggcaatgg cttacaatca atacaataaa
     6661 gacactacag acgaagaaat gaaaagtatt gtgcggcaat tgatttatca gtatgtaaaa
     6721 tatcacgata aagaaggtgt gtttttgcgt aaggcaacta ataaattcga aaaattttcc
     6781 tctaaagatt ctgtatattt taaacgctat tcttacaata ctttacaaaa atattttgat
     6841 gacaaatggt tcacaaaacg ctatcaaata gcttacgata aagtaaatga acccgatgaa
     6901 gtgcttactg catcacaaaa aaaattgaaa gcaaaaaaac gcaactatga agataagcat
     6961 ggtttgtcgc tgggtaaaga ttctataatg attatcgatc caacatatct gcaattcgat
     7021 ttacgcaaca gatcgaaccc gttacactta caagccagtg aaagcaattt acttgaatac
     7081 tataacacca ttcgtgatat cagtaagaaa gtagatttga aaacacaaat aattactccc
     7141 tatgaattga atgcacagga tatagaaaag ttcaacacgc tgatgacatt gaaagatgta
     7201 atgcgagaat atgaaaaaaa tgatgacgtg tttcttatac catttgatta tagcgccatt
     7261 gaaggcattc gcaaaaaata caacatcaat tacattgccg atatgggggt gctttcggca
     7321 aggttgaaaa aaccaagcga aaaatatttt acttcctgtg ttgctatgtt gtgttacccg
     7381 cttattccat ttctggtata tgggcttgct aaaccggagt atgaaaccta taattttatt
     7441 ttgctttacg atttagaaaa ttttgaacag aaaactatct tacatcgcat gaataataaa
     7501 gacaaaatat cggttacaag ttcggcacta tatttcaatt ttttacagat taaacgcgaa
     7561 tcaaaatatt aatatttaca acaatgaaaa aaaataatat tttccttttt gcatttgctt
     7621 tattaatgct ttcacaaaat gtacatgcac aatcaccggg atatttaggt aagcgggttg
     7681 ccatatctgc cgaaacaaat ttttgtcctt tggttggtta cttaatcaac aacgatgaaa
     7741 ttggtgattt caattggcgg tatgcaggcg gtatcgatta tgttgtttcc aattatcaaa
     7801 ctattggatt gaaagttgaa accatgaacg atgattttaa atacaaaagt tatgttactt
     7861 ccgatgataa ttacagtagt tcttattact attacgacca aacatataaa gcaactttaa
     7921 atacaacggc cataatattt tcattaaaaa ctttcaactt aaataaaggt gccatagctc
     7981 cactgggaaa ttatgctgct ttggaattag gttacttgtt cggcaatttt gatgttaatg
     8041 attttaatgt tagttcaacc acaaagcaat tggaagcttt tttattcacg tatggaatga
     8101 gttcgcgtgt tatatacaag agccgcgttt ttgtagacat tggttttaaa gccggattag
     8161 ccataaacgg aaatgcaaaa tattatttgt ccgaagtatc gaacattaaa caatataaag
     8221 cgcttgaaaa acgcagcgct accggcacta tgcttaccaa ttatttattt tcaatacaaa
     8281 tggctatagg gtttattcct tagggaaatg tttctgcgtt aagaattatt ccgaacacat
     8341 cacttcaata atttttcctg caaaaacaaa atcatggccc attgctgaaa gtcgatgttt
     8401 acttttttac gaaaaccatg tccttcgtct ttggcaacaa ggtaccaaac ttcaccacct
     8461 ttttcagcaa ttttcacttt catttgttcc gcttccgatg caggcacacg ggggtcgttc
     8521 aagccttgta taataaataa tggcttggta atttttgatg cattgcttgc aggcgaaatt
     8581 ttcgtgagaa attctttcat ctttggttcg cgctcatctc catattctac acgcctcaaa
     8641 tcttttctat aatcttctgt attggtaaga aaagttacaa agttgcttat acctaccaca
     8701 tcgatgccgc acttaagttt atcgttataa ttggttaaag aagccaacac catgtatccg
     8761 ccatatgagc caccatacac agcaatgcgc gatgcatcta attcgggctg ttgtgcaatc
     8821 cagtcgagca atgcaccaat gtcttttaca gaattttcgc ggttaaagcc attatccgat
     8881 ttcaaatatg ttttgccata cccttcggaa ccacgcacat ttggtgcaat tacagctatg
     8941 cccaattcat tggttaagta atttatatag gcattaaagg taggtaacga ttgaccctcg
     9001 ggtccaccat gaattaaaat aacaacaggt aattttgcat cagccttttt cggtttgtaa
     9061 taccatgcag gtatttgctg cattgcaccc ggaggcttgt cgaaagtttt ataacttatc
     9121 aacgttggcg ttacaaatgt gtttgggttg agtccaccta cttcgctgaa tgtccattgc
     9181 tttaaaatat gcgcacccaa atcataagta taaatatcac cgggcgattg tggcgaatta
     9241 atagtcatgg caataacatc attcaccgga ttaaatttta tgccgccaat tacaccaaca
     9301 ggcaaatcgt ttatgggtat atattctttt gtaagtgcat tcatttcata caagttcgaa
     9361 ttgccattgt tgtttacaga aaaaacaatc ttcgaacgat ttttattttc agccactctc
     9421 tctacattcc agggaataga ttgagtaata gaggtaaatt ttttggtagc gatgtcataa
     9481 tatttcaatg tcataaattc gctgccttca tcgtttacaa taaacaaccc tttgccatcc
     9541 tgcgaaaaaa ctacactgcc atacgaaatc tcttctttcg atttatttat ttgttcggtt
     9601 aaacctgttg caatatcaag caaataaatt tcggagcggt tggcagagat atacaacttc
     9661 actaccatct ttttatcatc gggcgaccag tcggccacac tccacgagcc accattttgc
     9721 aaaattattt ttcctttatc gggcgtttgt gtatcgtaca aatacaagtc gtaatcctta
     9781 ccatttctac gtgtgctcac cacacaaaat tggtgcccgc tattgctaaa cgaaggcgaa
     9841 gagttttgcg aacggccacc atcggaaatc attttgtacg agccgtttgt caagtcgaaa
     9901 acaaagagtt gcgaaaattc gttacctcct acatccttcg tatacataaa tcgttgtgtg
     9961 cggggtaatg gaataaatac accaccattt acaggctctt tgaaaaaagt gatttgctta
    10021 cgtgccccac ccgcatggtc tatgatgtgc agttgattta catcgccaaa gcgcgttgaa
    10081 agtaaaatgg acttaccatc ggcactccag tctgaaaatg aagctgaacg cgtattctgg
    10141 tattggttca ttttttcgcg gatgtcggct ggaatttcgg gtataccttc cattaccaga
    10201 tttcccactt cgcgctttgt tgcttgtgca aaaatgccag tggaaatatt ggcaacaata
    10261 cagagtgtaa acaacacttt aggaaaaata atttttttca tttgagtagg aaatatttat
    10321 ttaagtaaag ttattttatg agaaatggct tcggtgctgg tatttatttt cacaatgtaa
    10381 agaccatttt caaaagccga taaatctatt ctgttttgag gtaaagcatt tttcatagac
    10441 atcaataatt gaccatgctg attaaacact tcaataattt taagttcatt tttcaaatca
    10501 ttgatgatga aaaaaccact actgggattg ggatacaatt gcaatgttga agaaatattt
    10561 tcggccacac cggtactttg atttatattt atattatcca aatacaaacg attgccataa
    10621 ccatttatat tttcgaaacc aaattgcacg gcagtattat ttgccacagc cgctaacgaa
    10681 agttggtaag taatccattc attattggcg ggcgcatact gcgattgtgt gctgggtgca
    10741 gtggctaaat cgctgcctcc ttttttaaat attgatgtac gagtaactcc acaatctgtg
    10801 gtataaaaca cattaagagt atctgcatag ccggggaaaa tggcataagc cacatcaaaa
    10861 aacaaagcag gattggcaag cattgtaagg ttgtatggtg cagttaatat tacatcactt
    10921 tgaccgggct gattgtaaga ataattattg atcacggccg aactgccact aaggccataa
    10981 ccaccaacag aactactttg agaccatgta gcttgtccat cagggttcaa aattttccaa
    11041 tcggttactg ggaagttatt ctcgaaacct tcggtaaatg gtaccgcttg tgctacaggt
    11101 atggtaacat ttacataatt tgttttgagt aatgtatcgg tgccaaaagc attatttact
    11161 atcagttgca catcgtatac acctatagtt gcgtatgtga tttttggcga tgccaatgtt
    11221 gatgttgatg gtgtgccacc gggaaaactc cacgtccaac ctggtgcggc attgatggaa
    11281 gcatcgctaa attgtattga ttcgccaggg caaattgaaa tcttatcgga aacaaaattt
    11341 gcagtaggcg atgcttgcaa gggcacatac aaatcacttt cccacaaacc acggccgtat
    11401 gatgcagcac gaattttatt ggccacataa tttatttcca attccgaaat cgaaacattg
    11461 ggcaatccat tccagaaagg ttgccagttg cttagcgtgc tatcggtata aaaaacacct
    11521 atatcggttc caacatataa ggcatcgttt gtcccggttt ggtaaacaat agaattggta
    11581 gaaacattaa gcaaattgcc cgaaatattg gtccatgttg taccggcatc accactgtaa
    11641 aatattttat tgccgggcga agaacccgaa aagcaaaccc atactctatc ggcattggaa
    11701 ggatgaatgg caatagaatt aatagaagcc gaaccattag gaaggttaga agtaatatca
    11761 atccaattat ttccgccatc atcagtatac atcagtttat tatttactat cacataaatc
    11821 acattgctgt tacttggtgc aatattgaaa gccgaaacat ttgaagccaa agcaaaattc
    11881 gaaatctgtg cccagttatc gccattatcg gtactcttcc acatctcggc atagcctgca
    11941 taaattgtat tgctattatt tggatcgagc atatatggtg taatccaatt tccatttccc
    12001 atttggtttg ttgaaattgt attcatcgat tggccgccat cgtcactgcg gttaagccat
    12061 ccgccttgcc cacatgcata taaaatattt gggtcgtttg ggtcgagggc tgtttccata
    12121 ccatcggcac caagcacatg cgtccattgc ccattgttat aataattgca gccattgtct
    12181 tgcgcaccgg ccataatttt tgtggggtct gttgccgaag cacctaacct ataaaattgt
    12241 gcaatggtag cacctgcggt aatatcggtc catgtgttac cataatcgtc actggtaaaa
    12301 acgccaccat cgcaacccac ataaagttta tcgccataaa aatccatgta atgttgatcg
    12361 gcatgaacgt aagggtaatt agctccggca tcgtaaaacc aatgtgtgcg aataaaccag
    12421 ttgataccac catcggtcga tttccaaata tttattccgc ctgcataaat ttcatttttg
    12481 tttgatggag atacgcacaa agtcatatca taccaactct gccctcctgt tccatttcca
    12541 gtttcggagt aagtaaaaat attgggtgtg ctactttgtt gcgaaaatgt aaggccgcca
    12601 tttgttgatt tatataaacc gtaaaaccca cttgacgcag cagctccggc aactatatac
    12661 acatagttag catcggcagg tgttactcca atttgcaaat ggtttacatc ggccgaaagt
    12721 ggcaatccgg cagtgattac tgtccatgta ttgccaccat cggtagagcg aacaaaactt
    12781 gtacccgaag caaaaacaat attcgtatcg cccggcataa attctacatc acgataccta
    12841 ccactgtata ccttggtcca tgttgcaccg gcattgttgc ttttgtaaat accgtttgaa
    12901 gttgaggcaa tgagtgaaga agtgtcaccg ggaaaaatta atattttcga cacgttgtat
    12961 gcctgcgata atgtccatgt taatccggtt tgattccagg taatgccacc atctgtagat
    13021 tttaatatgc ctacaccata aatatcaccg gcatctttat cgccagtgcc catgtatata
    13081 gtattggaat tacccagcgg aacagctatt gccgatacac caagtgtagg aagctgatcg
    13141 gtattcgatg cccatgtgtt gccggtatcg ggcgaatgcc acaacccacc cgcaggtgca
    13201 ccaataaaaa tacttgctgc attgtttgga tcaacataca ctacattcac acggcccaac
    13261 cccggattgt aacttgtgct ttgccacgat gatggaccca gcgtagtcca gttacctgca
    13321 acattggttt ggtcgatgcc attttttttg caataatttt gaaactcgtt ccacacctga
    13381 tcgccatcga acatgcgccc cgtagggtat acacgcggtg cagtaaaata ttcccaacgt
    13441 ttaaaaactt cgaaccccgg tatttcctgt tcggtttctt tggaaaacag acgtgttaac
    13501 tttttgtgcc acacattctg cttacgcact tcgttgttgt aatatttata aaattctttt
    13561 tgtgtgtcgt aaaaatttgc attgcgatca tgcatcatgt ttacccacga ttgcgcattg
    13621 caaaattgtg tagtaagaaa taagaacaag aataattttt tcatagtgat aatttacttt
    13681 ttgtgaactt tttaaaatga aaaacaaaag taattttcgt aattgatgtt tcaatacaaa
    13741 ttaaaaatta aatcgtccat ctttcttggc aaaataattg atgcaatctc attgccccac
    13801 acatgagttt atattaacaa caaatctatg ttgtttgcta ttgcagaagt tgaaaatcta
    13861 attgtaatat tgttctgata tttctaatac ttaaacctaa acacttatgc gcaattttta
    13921 ttttctcatt gtttgtttgt ttgccattca aatatcaagt gcacaaataa ttggctccga
    13981 taccacttgt gcaggatata tttatcaata cgactttact tatcccgctg cagcatctta
    14041 cacctggaca gttcctaacg gttggtatat acttagtggg caaggaaccg gaagtgtaca
    14101 actgtactgc aacgtatcgg caggtaatat ttgtgttgat gctctggatt cgtcactcaa
    14161 tattattaat aatgactgca aacccgtaac cctaggtggt ggtggtagtg gatggtatgt
    14221 ggatcttact ccggcaggcc catgtatatg ctcaccaaat tttttagtaa tagttccaaa
    14281 tggcacagga ggtaattgtg gcggatgtgg aaatggaacg ccaagtgcaa gttcatacta
    14341 tgccgcctat gataatccat ggcctacagg aaattatctt ggcccaattg gtcccggtgg
    14401 tgttacgatt ggtggcccgt ttggcggcac ttattatgtg tactatatcg ataccatttt
    14461 aggattttca aatgcatttg tagttgatgg tggcgcatgc ggtggttcgg taaataatgc
    14521 tattactgtt tttccatgtt ttcctcctat ggttccactg ttacaatctc ctgcatcaat
    14581 ttgcccgggc gataccttca ctgtttatgt tgatcccagc ctcttaattt tttgggcaag
    14641 ttctttatgg aacccaagtg ggaataatat ttctgtaata aatacttaca ctgattctat
    14701 acaatgtatt ctgttataca atcagcctgg cagtggcatt caggctttct tttctgatat
    14761 ttatcaatgc ccttatggtg caataatagc tttaaatatt gctaccaatt gtagcccaaa
    14821 cgacccttgt cttattgccg catatccgct cgatggcaat gctattgata ccaccatcaa
    14881 tggcaacaat gcaacattca acaatactca acttgctacc aacagattta atattgcaaa
    14941 tacagcagca caattcaatg gcattaataa tagaatagac cttcccgatg attttgattt
    15001 cccacagcgc acatgggctt tttggtttta tgctgatact attactacaa attatcatga
    15061 aatatttgat gacgataatg tgtacattca aaatgcacaa acggaagttt ttttgcatga
    15121 tgattcaacg ggggtaaaaa aactttatgc tgctattgga ttgaatcaat atataattcc
    15181 tgtaaacgaa aagcaatggt atcaccttgc acttgtgcgc gataccggtg aaacacgttt
    15241 ctacctcaac ggatgtttaa tgcaaatagg tacaaacatg gccaacgacc atagttttga
    15301 cggagctata tccgtatcgc ttgggtgcaa ccgttttcat aatggaaatt attttaaagg
    15361 caaactggac gatttattta tttatcaatg tgcacttacc gatagcgaaa ttgccatggt
    15421 agcagataca aattgttttg tggctacccc tgttgctgca ttacaatcga gcgatacaat
    15481 ttggtgcgat aaaaagtgta tagattttat tgacttgtcg caatttaatc ccactatatg
    15541 gcaatggact tttgccgggg cttcgcctcc aacatcaacc gaccaaaatc ctacaggcat
    15601 ttgttataac aattacggaa cattcgatgt aacacttgtg gcttgcaata atggtggttg
    15661 cgatactgta acgtatttaa attttattac cgaatatcaa ttgccgcctc aaccaactat
    15721 ttcttttgta agcgatacat tgttttgctc gcaagctaat agttatcagt ggtttataat
    15781 tcctaatcct atccccggag cagtgttgca atattactat cctatggtga acggttcgta
    15841 ttatgtagtg atatcggata gcaatggttg tggcaatgca agtaatacat ttattataag
    15901 tggaaccatt gaaaacaatt caaattattt tcaaaacttt tattgtgatg aagatggatt
    15961 atgcaccatt gaaaatttat tgcaaaatgg aaaggtagcg ttaagcattt ttgattttga
    16021 tggaaggaaa ttaaatgaaa aaataatttc agcacaaacc gaaactgtcg atttaaaatt
    16081 tttgccgaaa gggatttata tcgtacaact gatgagcgaa aaaaaattat ataatttcaa
    16141 aattgtgcgg taaataattt ttatgaaaaa tgaaaacata aagtcattca acaaaaaaca
    16201 ataatttcat acgctttcaa ttgtgccttc tattggtatc tgcgcaatcc acaagtattt
    16261 gaaatttttc gccctgggtt ggtgtccctg ccatgtccaa cggtttgata aagcaaagca
    16321 ttttgctggt gaatttgtcc actatagctt gtatattttt tgaagcatca ttaaaacgaa
    16381 ctctcaactt gtactcggtt ggcgtccccg cctaccgctg tggaataaca ttttatttct
    16441 atttgtaaga tagtggacag aacaaaaaat gtaaccctct ttttttggga gataaaacat
    16501 ttcgactaca acatgcggtt ggcggggacg ccaaccgagg acagaaaaat gtagcgcact
    16561 aaaatgctca atactttttg ttttcatggt gacgaataaa tttattttta cttcactatc
    16621 accaaagact tgtcctcagt tggcgtcccc gccaaccgct gtggaatagg catttatttc
    16681 tctttgttag attgtggact gaacaaaaaa tgtagccctc ttttttgggg agataaaata
    16741 tttcgtctac aacatgcggt tggcggggac gccaaccgag gacagaaaag taaaacctta
    16801 tttgcgaaag cttaacgatt gatgattaat ttttgccgcc aatgttggtt gtcggcttgt
    16861 gcatcaataa aataaatgcc gggaggtaaa tcgcttaccg taatggtagt ttcgcctcca
    16921 ctatttgcat tgattgtttt tactaattgc atagttgtgg aataaatatt taattcaact
    16981 tgcgaattta cgattggaat tattacattg attaagtcat ttgcgggatt gggaaataac
    17041 aaaaatttac ctgtacttaa atttaaatca gctattgaat tgggaatact cgaacacatt
    17101 gtatcaacca caactgcagc acttaacata ggtaaatagt aaacaactga ctgattggca
    17161 aaatcaaaat aatcataaat tacagagtcg caatgctgcg gattggtgct gccccaatag
    17221 ttgctgtcaa gataaaaatc ctggttaccg tattgtacat tgttataaat ttcaatattt
    17281 tcagggttta aaaaattatt atgtttcata tacaagctgt agttggccgg gtctgtttgt
    17341 tgccaggcat tgtcgatgaa aatagtgttt ggcgcaatat tatttttaaa atcattgcgt
    17401 tcaacatgca tacttactga cggatatgct aaaggaggaa ttaatttgca aattgagccc
    17461 aaagaatttg aattagagtt taaaatattt tgataaatat aaatagtggg atatgtagtt
    17521 acaatataaa gacatgttgc ttcattgatg cctatatttc catcaaacaa gttgtgatgt
    17581 atttcacccc ccaatggaca agctatacat gagccaacat caaatagatt attcaaaaaa
    17641 atattgcaaa aaatatatgc ttcactacta ctaggacaat aaaccaatga agcgccatat
    17701 ccagaaaagt cgttgttaac aaataaatta ttattaaaat aaaaattttg cccgtcaaaa
    17761 aatatcaaat gattcggccc atgattattt ctaaacacgt tattgctcac tattctatca
    17821 taggtagtat agtttccact aaaataaatt gcaccattat tcatctcaaa tgtatctgtt
    17881 tgaattttta tatttcctgt aagataataa ctatatgtgt tcattatacc aaaattttcg
    17941 caccccttaa tcaaacttga atcaatgata taagttgatt cgaaagaaaa aattccatta
    18001 gtaaaacttg agattatgcg gcagtaagac aagtatggcg cagagcgctc catatgaatt
    18061 gcaccataac ctattccccc tgcgtaaaga acgttacaat atttcataat actacctgat
    18121 tggtatttgc cctgagagtc aaaaacagca tcgatgcatg tatcagcaaa atgtatttta
    18181 gcccaatcac ctttttgtgg gtttgcctga ttagaagtaa aagtaatctg gtcttcaaag
    18241 gttccaattg caataagttc accatcaatt tgcagtgcct tgtcggtttc aaatttaact
    18301 attacacccg gttctattgt tagagtattg ccgtgagtta acagcgcatt accatttaca
    18361 atataaggat ttccagcaac actccaagtt gtatttgttg aaataaagcc actgataaat
    18421 gtttgtgcaa aggaatttat gtacacaata aaacaaaaac aaataagcaa acaagtttta
    18481 gttttcatct ttttcatttt aaataatatt gttggaaaga tagcaatatc tattgaataa
    18541 atattatttc gtaagataaa attctttcca tttaatattg taaggaaagc aggccgaaaa
    18601 ctcaaatctt tttatgctcg gttggcgtcc ccgccaaacg ctatggaata aaaataaatt
    18661 ctttgttaac tacgcaaata tacctactca aaatacatca aattgagtat ttcaactaaa
    18721 ttaatttacc gtttcttcgc actacaaaat taacgctcac aaataataaa attttaaata
    18781 caaaaacaga tgatgaaagg aatcggttta ttgatgatgc ttatcgggat gataggcaca
    18841 tcgtttgcac aattgcggaa cttaaatgta tcgccttcca atgcagacaa agtacaaaaa
    18901 gtagaaatac aaaaagtaca aaacgcgcac tattgttata gccatgaata tatgctgcaa
    18961 caaatgtcgc aaaaccccga catggtgaag gagcaagaaa agttggaaaa agaaacagaa
    19021 cgacttagcc gcttgaccaa tgaaaacgaa aatcaaaagc aagctgtaat taccatacct
    19081 gttgtgtttc acgttttgta taattcaact tacgagaata tttcggacca gcaaatacaa
    19141 tcgcaaattg accaatggaa tcgcgatttc aatagagcca attgggattc ggtttacatt
    19201 ccttcggtat tcaagagtaa atacacaaac atgaatataa aattttgcct ggcaaatcgc
    19261 acaccgggtg gcgcaccttc gaaaggaatt attcgtaaat caacaacaaa aacaacatgg
    19321 acaacaggga atgcggccat taaacggtcg gcacaaggtg gtgatgatgc ctggaataga
    19381 gacaactatt tgaacgtatg ggtggtaggt gctctatcgg gcaatgtgtt ggcttatgcc
    19441 acgtttccgg gaggccccgc tgccgatgat ggcattgttt cgtggaacga atgtttgggt
    19501 acaacaggca cggtaggtta cccatataca ctaggtaaag tgctcacgca cgaagtaggg
    19561 cattggctaa acctccgcca tatttggggc gatgatggtg gtgcatgtac aggtagcgat
    19621 caagtgggcg atacaccaaa tgcatgggat gcaaattatg gcgatcctac ctttccacat
    19681 gttacgtgta gcaatggccc taatggcgat atgtacatga accatatgga ttatgtaaac
    19741 gaccaaacaa aaatcatgtt taccaccggg caaaaaacac gcgccaccgc agcattaaat
    19801 actacacgtg ttgccataca aacatctaac ggttgtgtgg caccaaatgc aaatgcgttt
    19861 agcgtacccg ctggaatttt agctcgtggt gtaaacgaaa atagtgcgag catttggtgg
    19921 aatacagttg ccggtgctgc aagtttcgaa ttgcaatatc gtaagaatgg cactactgca
    19981 tggactacaa aaacggcagc acaaattaat tatcgtatca gtggtttaac agtcgccaca
    20041 aaatacgatt accgccttcg tgcaatttat gcaaatggca acagtgctta ttctgcaata
    20101 aaaactttta ctaccgcatc gaactctgca tgcaatggcg acccatttga gtttaatgac
    20161 acctaccaca ccccttgccc aatcaatagc ggaaaaaatt attatgccaa attatcggca
    20221 accgatatgt acgattggta tattgtaaaa agcgatgcca gcaaacctta tatacgcatt
    20281 acactttcga acttacaaca agattatgac ctgcaagcaa acctggccag tggtactttt
    20341 ttggcatcat ctacaaactc cggtactacc aacgatgttg taaatttggc tggcgcatcg
    20401 ggcgaatatt ttcttgtaaa tgtaaatata ggtgccaact tggttagcac agcttgctat
    20461 aaattaatag tggaatataa ggctactcca ttcttagcta atgatgatga tattcgcctt
    20521 caagagccat cgaacgaagc aattgtaaaa gttgatatga tgccgaatcc tgcgagtaac
    20581 gaagcacacc tgaatatact aaccaatgaa aatattgata tagctataag tgtgattgac
    20641 aatttgggta aagtagtgta tgcaaataat accgttgcaa tagaaggaga aaaccttatc
    20701 gaacttcctg taaatgaatt tacaaatggc ttgtatcaag taattgtttc gggaaataat
    20761 tttaccgaaa tgaaaaaact tatagtgaac aaaaaaaatt aatacgaaat aatatttcga
    20821 tattttattt cacatttttt ttaataagtg cagtcagcaa tggctgtact ttttttttct
    20881 cttgatggcg ttagcgctaa ccatccttag tctttcatcc ctggtgggcg gggatgcaat
    20941 ccgtgaacaa aacttaatag aaataaaaat tgatagtgac agctttcaaa ataaaattac
    21001 ttctctttcc attcctgcgc atcgggttct acgtataacc acttcacctc ggggctgcca
    21061 cttcttattt cgtgttcaag tttgtttatc agtttgctta actcgagtgc tgtaaaatta
    21121 tgtgcgcatt ttattttcat acacatcatt acttcgcccg gcccttgttg tatagtaatg
    21181 cagtttatta gttttattac ttcggcatgt ttttgtaaaa tatcatttac cgattgtaat
    21241 atttcgtcac ttgcactttc acctatgagt aaagatttca cctcgaccga taaaaaaatg
    21301 gctacacaaa ttaatatcac accaatacaa aatgagcccg cagcatcgta acggctatcg
    21361 cctgtgtaat atgaagcaag catggcaaca agcgccacta ttaaaccaaa aacagcagca
    21421 gagttttcgc caaacactac taccagatca ctatccttag tggcgcgcag gtatttcata
    21481 aaacttaact tgccacgtct tacattcatt tcaacaatgt tcgaatacat ggcataactt
    21541 tcgagcaaga gcgaaaaaaa taaaattcct atgccccatg atacatgctg aattggttcg
    21601 ggcatattaa atttgtgtat gccttcgtaa atggaaaaca tcccaccaat cgaaaataaa
    21661 agcatggcta ccataaacga ccaaaaatat attgcacgac cttgcccaag tggatgcctt
    21721 gctgttgctg ccttttgcga ctgtcttact cctaccagca aaagtatttg attggcacaa
    21781 tcggaaaacg aatgtattgc ctcagccagc attgcacccg agtgggtcat aaacgcagca
    21841 acggattttg atatggctat tagcagattt accaccaaac tttgaatgat gtgcgatgat
    21901 ttgttgtgtg gtttttttga catagaattc aataaaaaat aaaagtaaag taatataaat
    21961 ttcttgtaca aaactttcta agtgtgtgtc tgatttattt gtaagcgatt tgtcctcggt
    22021 tggcgtcctc ggttggcgtc cccgccaacc gctgtggaat accaatttat ttctgtttgt
    22081 tagatt
//
LOCUS       DAORQA010000020        21927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_604_length_21927_cov_29.733221, whole genome shotgun sequence.
ACCESSION   DAORQA010000020 DAORQA010000000
VERSION     DAORQA010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21927
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_604_length_21927_cov_29.733221"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..5890)
                     /locus_tag="PK736_02980"
     CDS             complement(107..5890)
                     /locus_tag="PK736_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRC32389.1"
                     /translation="MSKKELSIFATCLMVWCVSLMSQAQQMCGFDQMHKFKMQSDPAY
                     SAQINALDAQVLNIVQNQGAANKLATQVYTIPVVVHVIHVGEPLGTGSNVTDASIQNM
                     IASLNSSFANANGNGVDIQIQFCLASRDPNGNATTGIRRVDGNVYAGYNPNGVYSYPG
                     CGGISDAPIKAANGWNKDYYYNIYIVINICGASGYAELPYNSGTKDGMVINYSAIGNA
                     PEVAHETGHWLGLLHTYTGQSGFNCPPNANCNIDGDKVCDTPPILNSECGNTSTCVGG
                     VITNSTSNYMSTCFNTWDRFTQGQKERMRGFMTCYPRANLLSSLGCIPLASNDAGVEY
                     ILNPMQTSYAANCTGNGTITPIVNLKNYGTNSITSASIKYKIDNGTLNTYSWAGTLTA
                     GQNVNITLPDFTASLGAHTFLAYSILPNAVADGNGNNDSGSVNFNYVSATIINATATG
                     TSTICSGSNSGSATVSTTSKLEIKEDFEGVTDWILANDFNDNERWYVGTATANGGSKS
                     MYISNDFGVSNNFNPNSGYSTAHFYKDFYFPAGATNITFKFDWKCGTGTGSYDNLKLF
                     MATPDVLPIGYNSLDNVAQELGTYFGSGSFNTATISNITAAGSVKRFVFTFDNRVGLS
                     TNQPPPSVDNIIVSYTPASGGTYSYSWNTIPVQTNATATGLLPGTYTVTATHSTGCTG
                     TANITINSSPAITLTTSQVNIPCGNGNVGTITANASGGTPSFSYLWSNAQITATATGL
                     AVGTYTVTVTDGAGCTITASATITTNCTVSISAGGPTTFCFGGNVVLTSSLATGNLWS
                     NGATTQSITASTSGSYSCTNNGNTSNSIAVTVNPIPATPTISNSGATTFCTGGSVTLT
                     SSSATGNTWTPGGATTQSINVTSSGSYTVKVTSNGCTSSPSIAKVVTVNPIPSTPTIT
                     AGGPTTFCTGGSVTLTSSSATGNTWTPGGATTQSINVTSSGSYTVAVTSNGCTSLPSV
                     ATVVTVQACSISISAGGPTTFCQGGNVVLTSSQATGNLWSNGATTQNITVTSSGSYSC
                     TNGGNTSNTIVVTVNPIPATPTISAGGPTTFCTGGSVTLTSSSATGNTWTPGGATTQN
                     INVTTSGSYTVKVTSNGCTSSPSAATVVTVQTCGSVSITAGGPTTFCSGGNVVLTSSI
                     ATGNVWSNGATAQSITVTTSGNYSCTNGGNTSNTISVTVNASPSTTTSQTGTVSLCSG
                     SLINGPITGNAASLDGVDDYITIANPVSSATAFTFEAWIYPKANTSWMRVFDFGNSPG
                     VNMFLAAPSFSGYPRFAINIGSGEQIVESNTALSINTWQHIAVTLSGTTARIYIGGLL
                     TGTNAAFTYNPASLGATVNNWIGQSQYPDPTFNGYIDEVRFWNTSRTQTQLQANMNKT
                     IGVTTSGLVAYYRLDESTGTSVLELVSGTSTSTANNGATWQVPSTVPFNPSGYLWSTG
                     ASTQGINVTAAGTYTVSVTAASGCTATSAPVVVSSIKPVTAATITASGPTTFCTGGSV
                     TLTAPSRGNALQFDGVDDYAAAATPMIPTTGNYSILFWAKESLNQTGYREIISQGRKF
                     YIGRDNTGIIRIGDDWTNTGVNWPTDLLWHHYAVTRNSFNTFLYIDGNLVATKGSAIG
                     SPIVVNGTPNLFMVGSQFSGVCCENFNGQVDDVTIWNSVRTITQIKAAMNSTVSTASI
                     GLVAYYKLDEGAGLSVLNAVNSSNAVTLNNGPTWVVPSSLPYNINSYLWSTTATTQSI
                     TAITAATYKVTVTNNSGCTSTSTKKVVISNPSASFTANGPLTFCNGGSVTFTANSGTG
                     FTYKWKNGASYINNATSQTYVATTAGIYRVEITNSVGCSKLSGSKTVTINCREEHNTN
                     GFDGLNLTVEPNPAQNYINLIFDSEYENASISILNISGQQLYNKNMQTDNGAIEDVLD
                     ISFLPPAVYIVRVSTGDVTNYARFVKQ"
     assembly_gap    6123..6148
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6260..8506
                     /locus_tag="PK736_02985"
     CDS             6260..8506
                     /locus_tag="PK736_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRC32390.1"
                     /translation="MYRLKITTCHCLILIVFAHSAYAKSNVDSLFTLWNNKTLEAENR
                     LKAIDLIIKENYATSKPDSAFYYAQQEYEFAKKMGAKKYEASALIYQSQSYRARGDLN
                     KAMDYINIALNLCDSTNDTHNMAASLNLKGNIYAMKDKGDSALYCYEQCLDIANRTND
                     NEVLASLYISIGIYHKDKGEYKKAIEQYEKSMAISKLVNDKINLARSYGSIASVYQMQ
                     GLYIKAMEYYLIALKISEDNGNKMSVSKILSNIGVVYFYQKQHHKAIEFYKRALAIEQ
                     EMKNKNAESTLLNYIGKSYFNLENYDEALPYFTLALNLQHTAGYRKEEAKTLGSFSEL
                     YFKKNEITKAMEFAKRQLALHQEINDEQGSAVAYGNVGNCYLLLNDNVRAVHNFQQSY
                     NIANKLHLASELRNSSLGLYQSYKQKGEPKMALSMYEKYIQMRDSIINENNNKEIARQ
                     EIKYEYEKKSLVDSLNSFAKIQEMALNKKLEIAQKAKERNLIIFGAIGLVLISTLWYQ
                     RKINQNKLIMKQQEAQYQKELTYATIIGQENERKRLARDLHDEVGAMLSTIKMNLGVV
                     NMKLKKSGMHEDLTAPSRNLLDDTISNVRQISRDLLPPSLEEFGLAVALNEFVTGVEK
                     ASEIVVNKNFQPMQHRIEPERELALYRVMQEMINNALKHSQATQFDISMNEKDGILFL
                     SFADNGNGFDLQKTKTNTSNTRGLGLLNLETRVDSTGGTMKYITAPNAGTRIEIEVPL
                     LTTKLKAA"
     gene            8503..9150
                     /locus_tag="PK736_02990"
     CDS             8503..9150
                     /locus_tag="PK736_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC32391.1"
                     /translation="MIQIGLVDDHKLFRSGLRALITAFEKYEVVMEAGNGQEFINQLP
                     HHNLDVVLMDLEMPVMDGLATIKILKEEFPKIKVIIVSMHSEEKFIAHLMELGARGYL
                     LKNANPEDIDIAIQSVSETGYYFTEMVSQVLLHGLVKREKVKPTFNILETLTARETEI
                     VKLICKEYTTPEMADKLFLSPRTIEGHRNSIMEKTHARNTAGIVVFAMKNGLYEE"
     gene            9638..10291
                     /locus_tag="PK736_02995"
     CDS             9638..10291
                     /locus_tag="PK736_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32392.1"
                     /translation="MRFTLVAIILLISGLGCIHGQCIKNTITTSAKENSAIKGCQYYQ
                     AIFGVYLGSKKVGTLIAEKITNHQIITYAIHSEVKINLIIELKIEESIKDVFTKDLLI
                     SSVHIREVNDVQKVKNTVVNQGDTYFFNSDNKPTGGTSERITATICSLYFNEPVNETV
                     VYSESYQQFITMVSSKQNTYEMQLPNGTTTTYKYAGPKLMSVFSNTTWGDLKFVREN"
     gene            10305..11108
                     /locus_tag="PK736_03000"
     CDS             10305..11108
                     /locus_tag="PK736_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009090943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC32393.1"
                     /translation="MEKEYALITGASKGLGKSFALELSKLNINLILVGLPNEGLREII
                     SEINTSDIDVQIHETDLTLEHNVIEMTKWANANFKIFMLINNAGIGGSKEFIDADISY
                     LSKIIQLNVLALTLITRALLPNLLLRPKSYILNVSSMAAFSPIGYKTVYPASKAFVHH
                     FTRGLNQELKDTNVFVSVVNPGPMKTNEEVIKRITNHGALAKIGLLSPDKVAAISIRQ
                     LLKRDDMIMLNFGNGLSWLLMKIIPIWIRLPLMTRAVKKELLLEKIIAT"
     gene            11108..12151
                     /locus_tag="PK736_03005"
     CDS             11108..12151
                     /locus_tag="PK736_03005"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRC32394.1"
                     /translation="MNVLITGANGMLAHNIIKLLLQKGIKVRATIRDKRKSCLQQHPK
                     LELIEGDITDNAFIEKAVIGCTHIIHAAALTFQNKRNINLYRRVNVESTENLVKAAIK
                     YGTTKFAFISSANTLGYGSNQNPGTETREMKHPFTASEYAISKKEAEESILKYKEQIE
                     IIIVNPTFIIGGDNIDSGSGQIMKMGLNRKIIFYPPGGKNFAHVKDVAYGTWKAMQDG
                     KNGERYLLANVNLSYKDFFRKLLKQNNKKALLVPLPKFIMLFFGIAGDIYSMLGGTTQ
                     ISLTNCKALCVKNFYSNCKAQKHLGIAFSPLALGISKNEFQQNPKITLSKNETHNCAE
                     IASQFAMTNEEKS"
     gene            complement(12388..14472)
                     /locus_tag="PK736_03010"
     CDS             complement(12388..14472)
                     /locus_tag="PK736_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRC32395.1"
                     /translation="MLNEFLQVKQQAATHSLMNVTAEAIQQLLPTSDFEAMQNELQRV
                     HQFKMMIDNGDNFPDRDYTDISKDLALLKIDNSILSADQCLRIQQCLHVAQQIFSFFK
                     NRKGIYEPFEEIIGNLLYDNSMQLWIEEVFDSEGVIKSSASDELYKIRKSLSRKRSEA
                     DKLFQTIVNRYRKAGYISDTEESMRNGRRVIAIVAEQKRNLGGVIHDTSATGKTAYLE
                     PDELLPINNAILGLEQEERTEILRILKELTTKLRIKYFHIDAYFDIQKQCDLLRARAM
                     YAAQLGAVLPLLKDEPSIDIKNAYHPVLMLQNKLQGKTTIPFSLYLRNKNRIMVISGP
                     NAGGKTICMKAVALLQLMLQSGFLVPCAAHSEFGFFDKLLVDIGDSQSIEYELSTYSS
                     RLQKMNMFLHEANERTLFIIDEFGTGTDPTLGGALAEAILEKLNELKSIGIITTHFIN
                     LKVLADKTEGIINGCMLFDPEKLEPKYKLVVGKPGSSYTFLVAERSGMDKEIIKNAES
                     KVSKNHILLENMLRDVENQKQVIDKKTKNIAHAEEALDEMLEKYGKLSSQAEQSKQTI
                     DSKIKQTELRLIKQFDDKVIKFVNEWKNSKDKKAVLAKYERMHGKEKSEIAKVQSKEQ
                     QRIQQEYISKLKPGVFVHLKGGKTIGMIDSMDDKKVLVIFNGFKTHCDINNLIPIEME
                     DVPMGVRKGFSS"
     gene            14639..15487
                     /locus_tag="PK736_03015"
     CDS             14639..15487
                     /locus_tag="PK736_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRC32396.1"
                     /translation="MSNKEQLTELVTQTRRDIVRMVHAVQSGHPGGSLGCTEFMVALY
                     NTVMEYTPKPFNMDGNNEDIFFLSNGHISPLFYSVLARAGFFDISELSTFRKLNSRLQ
                     GHPTTHEHLPGVRIASGSLGQGISVAVGAALAKKMNNDTHTVYVLCGDGELQEGQIWE
                     AVMFAAAKKVDNIILTIDLNGQQIDGATKDVLPIGDVRQKFDAFGWQTFHMTNGNNID
                     EVISVLNDAKRACGNGKPVAIIMKTEMGAGVDFMMGSHKWHGIAPNDEQLQTALAQLH
                     STIGDY"
     gene            15695..17101
                     /locus_tag="PK736_03020"
     CDS             15695..17101
                     /locus_tag="PK736_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRC32397.1"
                     /translation="MKFSFPSTSKVFFATAFAIVITIFATHKSYSQKPTPAPPIETRI
                     LFLYDCSASMMAQWQSGNKYDVATKLLSHVVDSLQDIPNLQTALRMYGHTKKFPPQDC
                     DDTRLEVPFGYRNGYKIKGKLKEFKPSGTTPIALSLEACGNDFPKAAGRNIIILITDG
                     IEECNGDPCAVSYALQRKGISLRPFVIGLGVNKEFAKQFDCIGTYYDAKDEASFNDIF
                     KVVISQALNNTSAQINLLDIYKKPTETNVAITFYDMLSGKVMQNIIHTMNTMGVPDTI
                     KLDPLTTYRMQVHTIPPVYKDSIKLTPGKHTIIGVSAPQGDLRLILEDSYEYKNLKCI
                     IRKHGEMTTLNVQNMNSTERYIIGDYDLEILTLPRIIKNNIEVKQSHTTSVEIPKPGL
                     VNLVSSNPGIGSILVDRSGTWEWVCDIDDTTSKQTLTLQPGNYRLVFRPKASRETIYS
                     LEKNFNIVPGGSTTLQVN"
     gene            17469..19010
                     /locus_tag="PK736_03025"
     CDS             17469..19010
                     /locus_tag="PK736_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32398.1"
                     /translation="MNKYIIIVIIFFLVSESGVAQNRNNIWCFGNDAKITFTNGTAIA
                     DSTKCNSRGTCVSICDSLGNLLFYAAGSTDGLSAQYQVGGKAYNKENSVMQNGDSIMA
                     RLWYKEMVILPYIYSDSLYYIVSAPVTGKGGFFYSIIDMSQDSGRGAVIQKNVQLQGS
                     NFYANDGIAAIKHGNGRDWWVMVRDWKDTNNVYFFYKVTPNGVSLSHSQAIGDSLRPG
                     FFRIEPSDDGSKIACCNAGGLISVFDFDRCTGYLSSEISIEKESSTYGGLAPWYWDCE
                     LSPNKRYLYVSQEFTDFDTTNYLLRFDLQAANIPASRDTLYAGERRATAGLIELAPDG
                     KIYWACTYYPPGTFFYPYPDSTHNYITDNLSVINYADSSFCDFQPFSFYLGGKRTYYG
                     LPNNPNYGLGRLLGSPCDTLQWVGTPETPNPLQGGLNTTYISAWEKLFVNAQNIKGKN
                     VTITIYDTQGKLMYVAGKPPKGGLGVFGGYFTLDVDCNGWASGLYVVHLQTEKEMLST
                     KFVKE"
     gene            complement(19147..19926)
                     /locus_tag="PK736_03030"
     CDS             complement(19147..19926)
                     /locus_tag="PK736_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015273866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRC32399.1"
                     /translation="MKIVVRRFMQIEKWLAVIFLVNGLFTYANAQSFLKEQSKYARVQ
                     NAIETKHKLIFENLKTHHLTSDNFNLLITVFKAEMKLLLYAKSKTATTYTLIATYDIC
                     AGSGELGPKRAYGDNQVPEGFYYINRYNPASNYYLSLGISYPNASDKIKSKAKNMGGD
                     IFIHGNCVTIGCMPMTDDKIKEIYLYAMYAHNNGQTQIPVYIFPFKMDDKNFENYKLK
                     NKTNVALIRFWQNIKIGYDKFFQSQRQLNVTVDTNGKYVFK"
     gene            complement(19945..20352)
                     /locus_tag="PK736_03035"
     CDS             complement(19945..20352)
                     /locus_tag="PK736_03035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC32400.1"
                     /translation="MITYKIENDLDADEFITVLNVSTLGERRPVNERDRIEKMLQHAN
                     LIVTARSNGKLVGVARSLTDFAFCTYLADLAVDEAFQKQGIGKELIRQTKLAAPQAKI
                     ILLAAPAAINYYPHIGMVKHDYCFLLHDIDTLK"
     gene            20481..21809
                     /gene="ffh"
                     /locus_tag="PK736_03040"
     CDS             20481..21809
                     /gene="ffh"
                     /locus_tag="PK736_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRC32401.1"
                     /translation="MFENLSDKLDRAFKVLKGQGKITEINVAETLKEVRKALLDADVN
                     YKVAKDFTDKVKASALGQNVLIAVSPGQLMVKIVKDELTSLMGGEHSELNLKASPCII
                     LMSGLQGSGKTTLSGKLANFLKNKRQKNVLLVACDIYRPAAIDQLTVLGDQIGVEVYS
                     ERENKDAVKIAQNGIARARDLGKNVVIIDTAGRLAIDEQMMDEISRLKNAVKPTETLF
                     VVDSMTGQDAVNTAKAFNDRLDFDGVVLTKLDGDTRGGAALSIKSVVNKPIKFVGTGE
                     KLDELDIFYPARMADRILGMGDIVSLVERAQEQFDEKEAMELQKKIAKDKFNFNDFLQ
                     QLQQIKKMGNMKDLVGMIPGVGKALKDVEIDNKAFDRIEAIIKSMTPKERETPELINI
                     SRKKRIAAGCGMEIAEINKLIKQFDETRKMMKAFTTGNMKNMMNKMPGRR"
BASE COUNT         7018 a         4190 c         4250 g         6443 t
ORIGIN      
        1 attttaaaca ttctttaacc catatcatgc aattgaaatt cgaataagct caaaaaaaaa
       61 cactccgatt aaggagtgct ttttagttga aaataaattt caatatttat tgctttacaa
      121 atcgggcgta gtttgtgaca tcacctgtac ttacccttac aatataaaca gctggtggca
      181 aaaacgaaat atccaacaca tcttcaatag ctccattatc agtttgcata tttttattgt
      241 aaagttgctg accggaaata ttcaaaatac ttatcgaagc attttcatat tcgctatcga
      301 aaatcaggtt aatataattt tgtgcggggt tcggttctac agtcaaattc aaaccgtcaa
      361 atccatttgt gttatgctcc tcgcggcaat tgatggtaac cgttttcgaa cctgataatt
      421 tactacatcc caccgaattg gttatctcta cacgatagat accggcagtt gtagcaacat
      481 acgtttgtga agtagcattg ttaatatatg atgcgccatt cttccattta taggtgaaac
      541 ctgtgccgct gtttgctgta aatgtaacac taccaccatt acaaaacgta agagggccat
      601 ttgccgtgaa tgatgctgaa ggattgctta taacaacctt tttggttgat gttgaggtac
      661 agccggaatt gtttgtaact gtcaccttat acgtagctgc agtaattgct gttatacttt
      721 gagtagttgc tgttgttgac catagatagc tattaatatt atatggcaaa cttgatggaa
      781 ccacccatgt agggccatta tttaaagtaa ctgcattgct gctatttact gcattcaaaa
      841 ccgatagtcc ggctccttca tcaagtttat aatatgcaac aagtccgata gatgctgttg
      901 atacagtgct gttcatagct gcttttattt gtgtgattgt acgtaccgaa ttccaaatag
      961 taacatcatc cacttgacca ttaaaattct cgcaacatac gccactaaat tgactaccaa
     1021 ccataaacag gtttggagtg ccgtttacaa caatcgggct gccaattgca ctgccttttg
     1081 tcgctacaag gttaccatca atatataaga aggtattaaa tgaatttctt gttacagcat
     1141 aatgatgcca tagcaaatca gtaggccagt ttactcctgt gttggtccag tcatcgccaa
     1201 tgcgtataat tcctgtgtta tctcttccta tatagaactt gcggccttga gatattattt
     1261 cccgataacc tgtttgattt aaactttctt ttgcccaaaa taatatggag tagttgcctg
     1321 ttgtaggaat cattggagta gcagcagcag cataatcatc aacaccatca aactgcaatg
     1381 cattacctct tgaaggtgcg gttaaggtta ccgagccgcc tgtgcaaaat gttgttggtc
     1441 cgcttgcagt aatggtggct gctgtaaccg gcttaatgga tgatactacc accggtgccg
     1501 atgttgcagt acaacccgat gctgcagtaa cacttactgt atacgttccg gctgctgtta
     1561 catttatgcc ctgagttgaa gcccctgtgc tccacaaata gcccgaaggg ttaaaaggta
     1621 cagttgaagg aacctgccat gtagcaccat tatttgctgt ggatgtactg gtacccgaaa
     1681 cgagttccag cacggaggtt cctgtacttt catctaacct gtagtaagct accaatccgc
     1741 ttgttgtaac cccaattgtt ttattcatat tagcttgcag ctgggtttgt gtgcgtgatg
     1801 tattccagaa acgtacctca tcaatataac cattgaaagt tgggtcagga tattgagatt
     1861 gtccaatcca gttatttacg gttgctccca gcgatgccgg attataagta aaggctgcat
     1921 tggttccggt aagcaaaccg ccaatataaa ttcttgctgt tgtgcccgaa agtgttacgg
     1981 caatatgctg ccatgtatta attgataaag ctgtatttga ctcaacaatt tgctcaccgc
     2041 tgccaatatt tattgcaaac cgtggataac ctgaaaaaga aggagccgct aaaaacatgt
     2101 taactcccgg actgttacca aaatcaaata cacgcatcca actggtattt gctttcggat
     2161 atatccatgc ttcgaaagta aatgcagtgg ctgacgaaac aggattagca atagtaatat
     2221 aatcatcaac tccatcaagg cttgcagcat taccggtaat agggccgttt atcaaacttc
     2281 ccgaacaaag tgacactgtt cctgtttgac ttgttgttgt tgaaggtgat gcgtttacgg
     2341 ttactgaaat agtattagaa gtattaccac cgttagtgca actgtaattt cccgaggtag
     2401 ttacggttat gctttgcgct gttgcaccat tgctccatac attgcctgtt gcaattgatg
     2461 aggttaaaac aacattaccg cctgaacaga aagttgtagg gcctccagcc gtaattgata
     2521 cgctgccaca tgtttgaacg gtaacaacag ttgctgcaga tggtgatgaa gtacaaccgt
     2581 tacttgtaac tttcactgta tacgaaccag atgtagtaac gttaatattt tgagttgtag
     2641 cgccaccggg agtccatgta ttgcccgtag ctgatgatga agtaagtgta acactacctc
     2701 ctgtacagaa tgttgttggt ccgcccgcac tgattgttgg tgtagcaggt attggattaa
     2761 ctgtaacaac aattgtatta gaagtattac caccattagt gcaactatag ctgcctgatg
     2821 atgtaacagt gatgttttgt gttgtagcac cgttgctcca caaatttcct gtagcttgtg
     2881 atgatgttaa aacaacatta cctccctgac aaaaagttgt agggccacca gcagaaatac
     2941 taatgctaca tgcttgcaca gtaacaacag ttgcaaccga tggtaatgaa gtacacccgt
     3001 tacttgtaac tgccactgta tacgaaccag atgaagttac atttatgctt tgcgtagtcg
     3061 cacctccggg agtccatgta ttacctgttg ccgatgatga ggtaagcgtt acacttcctc
     3121 ctgtgcaaaa agtagtcggg ccaccggcag taattgtagg tgttgatggt ataggattta
     3181 ctgttacaac tttggcaata gatggtgacg atgtacatcc gttacttgta acttttactg
     3241 tatacgaacc agatgaagtt acatttatac tttgtgttgt agcacctccg ggagtccatg
     3301 tattacctgt tgctgatgat gaagtaagtg ttacacttcc accagtacaa aaagttgttg
     3361 cacctgaatt actgattgtt ggagtcgcag gtattggatt tactgtaaca gcaattgaat
     3421 tggaagtatt tccattgttg gtacaactat aactgcccga tgttgaagcg gtaatgcttt
     3481 gtgtagttgc tccgttgctc cataaattac ctgttgcaag tgatgaagtc aataccacat
     3541 tgccaccaaa acaaaatgtt gtagggccgc ctgctgaaat actaactgta caatttgtgg
     3601 ttattgttgc ggaggcggtt atagtgcaac ccgctccatc agtaactgtc accgtgtagg
     3661 ttccaacagc taatcctgtg gctgttgcgg ttatttgcgc attgctccat aagtaggaaa
     3721 atgacggagt tccgccagaa gcattcgctg ttatggtgcc aacatttcca ttgccacaag
     3781 gtatattaac ttgcgaagta gttaaggtaa ttgctggcga tgaattaatg gtaatattgg
     3841 ctgtgccggt acaacctgtg ctatgtgtgg ctgtaacagt ataggttccg ggcaacaatc
     3901 cggttgccgt agcatttgtt tgtacaggta ttgtattcca ggaataagaa tatgttccgc
     3961 ctgaggccgg tgtgtagcta acaataatat tgtcgactga tggtggtggc tgatttgttg
     4021 ataatccgac ccggttatca aaagtaaaaa caaatctttt gacactacct gctgcagtaa
     4081 tgtttgatat tgtagcagta ttaaaacttc ctgaaccaaa atatgtgcct aactcttgag
     4141 ctacattgtc caatgaatta taaccaatag gaagtacatc aggagtggcc ataaacaatt
     4201 ttaaattatc ataagatcca gtaccagtgc cgcacttcca gtcaaattta aaagtgatat
     4261 tagtagcacc tgccgggaaa taaaaatcct tataaaaatg agctgttgaa tacccgctat
     4321 ttggattaaa gttatttgac acgccaaaat cattggaaat atacattgac ttagagccac
     4381 cattagctgt tgctgtacct acataccatc tctcattatc attaaaatcg tttgcgagta
     4441 tccagtctgt tacaccttca aaatcttctt taatttcaag ttttgatgta gttgaaacgg
     4501 tagcagaacc cgaattgcta ccactacaaa ttgtcgatgt tccggtagct gttgcattta
     4561 taatggttgc agacacataa ttaaagttga cactgccact atcattatta ccatttccat
     4621 cagcaactgc gttaggcaaa atgctgtatg ccaaaaatgt atgtgcacct aaagaagctg
     4681 tgaaatcagg gagtgtaata tttacatttt gaccggcagt taacgttcca gcccatgagt
     4741 acgtgtttaa tgtaccgtta tctattttgt attttatact tgccgaggtt atcgaattgg
     4801 ttccataatt tttaagatta actatcggtg taattgttcc attgccggtg caatttgctg
     4861 cataagatgt ttgcatcgga tttaaaatgt attcaacacc agcatcgtta cttgctaaag
     4921 gaatacaacc caatgaagat aacaaattcg ctcgtgggta acaggtcata aatccgcgca
     4981 tcctttcttt ttgtccttgc gtaaatcgat cccatgtatt aaaacatgta ctcatataat
     5041 tacttgtaga attagttata acccctccta cacaagtaga agtatttcca cactcagaat
     5101 ttaaaattgg aggtgtatcg caaactttat caccatcaat gttacagttt gcattaggag
     5161 ggcaattaaa acctgactga ccagtatagg tatgaagtaa tccgagccag tgacctgtct
     5221 cgtgagctac ctctggggca ttaccaatcg cagaataatt aatgaccatg ccatctttcg
     5281 tgccgctgtt gtatggtagt tcggcataac cacttgcacc acatatatta attactatat
     5341 agatattgta gtaataatct ttattccagc cattggctgc tttaatgggt gcgtcagata
     5401 ttcctccgca accgggataa gaatatactc catttggatt gtaaccagca tatacatttc
     5461 catccacacg tcttattcct gttgtagcat ttccattagg atcgcggctg gcaagacaaa
     5521 attgaatttg aatatcaacc ccattaccat tggcattggc aaagctactg tttaaagaag
     5581 caatcatgtt ttgaatgctt gcatcagtta cattgctgcc tgtacccaat ggctctccaa
     5641 catgtataac atgtactacc acaggaatag tataaacctg tgtagcaagt ttgttggccg
     5701 ccccttgatt ttgcacaatg ttaagtacct gagcatccaa tgcattaatt tgagctgagt
     5761 atgcaggatc actttgcatt ttaaatttat gcatctgatc aaaaccgcac atttgctgcg
     5821 cctgagacat aagcgataca caccatacca tgaggcaggt tgcaaaaatt gataattcct
     5881 tttttgacat ttgatttaat tttttaattt ttgagattat tgatgaaggc attttatttt
     5941 acagcaatta gtttttcaaa aaaacaacat gacaaaagta agagagaaaa aagggagtgg
     6001 ttagcgtaga aatacctaaa atgtaaaatc acgtatttgt actcaaaacg atagggcaac
     6061 tttacaattt attttttctg tttgcaaatt attatacctt aaatttgtct attgttatct
     6121 acnnnnnnnn nnnnnnnnnn nnnnnnnnac cgggcatacc atctgcaaaa aaagttggag
     6181 atttattaaa tatattgcgt aactttttta atgcaaaaat aattgtactt ttaaatttcc
     6241 attttaaaaa ccaaaaccta tgtatcgatt aaaaataact acttgccatt gcttaatatt
     6301 aattgtgttt gcacatagtg cttatgcaaa atcaaatgtt gacagcttat ttactttgtg
     6361 gaataacaaa accttagagg ccgaaaacag actaaaagcc atagacctga ttatcaaaga
     6421 aaattatgct acatcgaaac ctgatagtgc tttttattat gcacaacaag aatatgagtt
     6481 tgcaaaaaaa atgggggcga aaaaatatga agcctcagcc ttgatctatc aaagccaatc
     6541 gtaccgtgct cgaggcgatt taaacaaggc aatggactac ataaacattg ctttgaatct
     6601 ttgcgatagc acaaatgata ctcataacat ggctgcaagt ttaaatttga aaggaaatat
     6661 ttatgcgatg aaagataagg gagatagcgc gctttattgt tatgagcaat gcttggatat
     6721 tgccaataga actaatgata atgaagtgct ggcaagttta tatataagta taggaattta
     6781 tcataaagac aaaggcgaat acaaaaaagc aatagaacaa tatgagaaat cgatggctat
     6841 ttcgaaactt gtgaatgata aaataaacct ggcacgcagc tatggaagta tagcatcggt
     6901 ttatcaaatg cagggcttat atattaaagc aatggaatat tatttaattg cactaaagat
     6961 aagtgaggat aacggcaata aaatgagtgt atcaaagata ctttcaaaca ttggggttgt
     7021 ttatttttat caaaaacagc atcacaaagc aatagaattt tataaaagag ccctggctat
     7081 agagcaggaa atgaaaaata aaaatgccga atccactctt ttgaattaca ttggtaagtc
     7141 gtatttcaat ttggagaact atgatgaagc cttaccatat ttcacacttg cactaaactt
     7201 acaacacact gcaggttacc gtaaagagga agcaaaaacg ctcgggagct tcagcgagtt
     7261 atattttaaa aaaaatgaga taacaaaagc aatggaattt gcgaagcggc aactggcttt
     7321 acatcaggaa attaatgatg aacaaggtag tgcagtagcg tatggcaatg ttggcaattg
     7381 ctatctgcta ttgaatgata atgtgcgtgc ggtacacaat ttccagcaat cgtataatat
     7441 tgccaacaaa cttcaccttg catctgaatt acgaaatagt tcattgggac tatatcaatc
     7501 atacaaacaa aaaggtgagc cgaagatggc actttcgatg tatgaaaaat atatacaaat
     7561 gcgcgacagc ataattaatg agaataataa caaagaaatt gccaggcagg aaataaaata
     7621 tgagtatgaa aaaaaatctt tggttgatag tttaaattcc tttgcgaaaa tacaagagat
     7681 ggcgttaaat aaaaaacttg aaattgcaca aaaggcgaaa gaacgaaacc tgattatttt
     7741 tggtgccata gggctagtgc ttatttctac gctatggtat cagcgaaaaa taaatcagaa
     7801 taaattgata atgaaacaac aagaagcgca atatcaaaaa gagttaacct atgcaaccat
     7861 tattggtcag gaaaacgaac gcaaaagatt agcccgcgat ttgcacgatg aagttggtgc
     7921 aatgttgtcg accataaaaa tgaatcttgg tgttgtaaat atgaaattaa aaaagagcgg
     7981 aatgcatgaa gatctcactg caccttcgcg caatttgctt gacgatacca tatcgaatgt
     8041 gcggcaaatt agcagagatt tgctgccacc ttcactcgaa gaatttggtc ttgcagttgc
     8101 gctgaacgag tttgtaactg gcgttgaaaa ggcttcggaa attgttgtaa acaaaaattt
     8161 tcaaccaatg cagcatcgta tagaaccgga acgtgaattg gcattgtatc gtgtaatgca
     8221 ggagatgatt aacaacgctt tgaaacattc acaagcaaca cagtttgaca tttcgatgaa
     8281 tgaaaaagac ggcattttat ttctttcatt tgccgacaat ggtaatggat tcgatttgca
     8341 aaaaacaaaa accaacactt cgaacacacg cggcttagga ttgcttaatc ttgaaacccg
     8401 ggttgatagt accgggggca caatgaaata cattaccgca ccaaacgctg gcacccgaat
     8461 tgaaattgaa gttccattat taacaactaa attgaaagcg gcatgataca aattggattg
     8521 gtagacgatc acaaattatt tcgatcgggc ttgcgtgcct tgattacagc attcgaaaaa
     8581 tatgaagttg ttatggaggc aggcaatgga caggaattta ttaatcaatt gcctcatcat
     8641 aatttagatg tggttttaat ggatttagaa atgccggtga tggatggctt ggcaacaata
     8701 aaaatattga aagaagaatt ccctaaaata aaagtgataa tcgtatcgat gcattctgaa
     8761 gaaaaattta ttgcccattt gatggagtta ggggcacgcg ggtatttgtt aaaaaatgcc
     8821 aatcctgaag atatcgatat tgctatacaa tcagtatccg aaacaggtta ttattttacc
     8881 gaaatggttt cgcaagtatt gttgcatggc ttggtaaaaa gggaaaaagt gaaaccaact
     8941 ttcaatatat tggagacact tactgcccgt gaaacagaaa tagtaaaact aatttgtaaa
     9001 gaatacacaa cacccgaaat ggcagataaa ctttttctta gcccgcgtac aattgaagga
     9061 caccgtaaca gcattatgga aaaaacccat gcccgcaata ctgcaggcat tgttgtgttt
     9121 gctatgaaaa acggcttgta tgaggagtga cgatcattca agaaccaata caaaatagag
     9181 aattttagtg tcctcattta ttttttgttt ttcaaaggca tgagcgactc tgaaataaat
     9241 gcaaattttt ttctacccct catttttgtt ccatcttata ttatgcaaaa tatttattca
     9301 tacgattcac ctatatcaga ggagaaggaa aaatattgat attgatatct ggcatgaaac
     9361 taaggaataa aatttttcaa gtatttgatt aatattttag acacgaaagc cagactatat
     9421 atgcaggcta aatctaaatt caaaattatc taaaccttca gataaatcgc accacaatga
     9481 ttggctggcg taaacaaata ctttgaggct atgcggccaa aataaatatt cggcattcat
     9541 tagcgttaat ggaataccgt aataattttt tctttttgct cataggggta tatcaattgt
     9601 gggttgtcat acttataacg aatacaaatt caaacatatg agatttacat tggtagcaat
     9661 cattttacta atatctggct taggctgtat acatggacaa tgcataaaaa acacaattac
     9721 aacttcagcc aaagaaaact ctgcaatcaa gggatgtcaa tattatcaag ccatttttgg
     9781 ggtatacctt ggttcgaaaa aagtaggaac attgattgct gaaaaaataa ccaaccatca
     9841 aatcattaca tacgctattc attctgaggt aaaaataaat ttgataatag aattgaaaat
     9901 cgaagaatcg ataaaagatg ttttcacaaa agatttactt atctcttcgg tacacattcg
     9961 cgaagtaaat gatgtacaga aagtaaaaaa taccgttgtt aatcaaggtg acacatattt
    10021 tttcaacagc gataataaac ccacaggtgg cacaagtgag cggataaccg ctaccatttg
    10081 ttcactgtat ttcaatgagc ctgtaaacga aactgttgtt tattccgaaa gctatcagca
    10141 atttataaca atggtgagca gtaaacaaaa cacttacgaa atgcaattgc ctaatggtac
    10201 caccacaacc tacaaatatg ccgggccaaa attaatgtcg gttttttcga atactacctg
    10261 gggtgattta aaatttgtac gcgaaaacta aaaagaaaaa ataaatggaa aaagaatatg
    10321 ctctgattac cggagccagt aaaggtttag gtaaatcatt cgcactcgaa ttatcgaaac
    10381 taaacatcaa cctcatttta gttggcttgc caaatgaagg actgcgcgaa ataatttcgg
    10441 aaataaatac aagcgatatc gatgtgcaga ttcatgaaac agatttaaca ctcgaacata
    10501 atgtgattga aatgacaaaa tgggcaaatg caaattttaa aatttttatg ctcatcaata
    10561 atgcgggtat aggcggtagt aaagaattta ttgatgccga cataagttat ctgagtaaaa
    10621 taattcaact aaatgttttg gccctgactc tgataacgcg ggcattgtta cccaatctgt
    10681 tgctaaggcc aaaatcatat atcctaaatg tttcgagcat ggccgcattt agtcctattg
    10741 gttataaaac cgtttatccg gcatctaagg catttgtgca tcatttcaca cgtggcctca
    10801 atcaggaatt aaaagacaca aacgtatttg taagtgttgt taatcccggc cctatgaaaa
    10861 caaacgaaga agtgattaaa agaattacaa atcatggcgc acttgcaaaa attggacttc
    10921 tatcacccga caaagttgcg gcaatctcca tcaggcaatt attgaaaaga gatgatatga
    10981 tcatgctaaa ctttggcaat gggctttcgt ggttgctgat gaaaataatt cctatctgga
    11041 tacgactccc acttatgaca cgtgccgtta aaaaagaatt gttactcgaa aaaataattg
    11101 ccacataatg aatgttctta ttacaggtgc aaacggaatg cttgcacaca atatcataaa
    11161 actattattg caaaaaggaa ttaaagtaag agcaacaatt cgcgataaaa gaaaatcgtg
    11221 cttacaacaa cacccaaaac tcgaactgat agaaggggac attaccgata atgcttttat
    11281 cgaaaaagca gtgattggtt gcactcacat aattcatgct gcggcactca ctttccaaaa
    11341 caaaagaaat ataaatctat acaggcgtgt gaatgttgaa agcactgaaa acttagtgaa
    11401 agccgctata aaatatggta caactaaatt tgcttttatc agtagtgcaa atacattggg
    11461 atatggcagt aaccaaaacc ccggcacaga aacacgtgag atgaagcacc cttttacagc
    11521 ttcggaatat gctatttcaa aaaaagaagc tgaagaaagt atactaaaat ataaagagca
    11581 aattgaaatc atcatcgtta atcccacatt tattattggt ggcgataata tcgattccgg
    11641 ttcggggcag attatgaaaa tgggactgaa cagaaaaatt attttttatc cacccggagg
    11701 aaaaaatttt gcgcatgtaa aagatgtagc ctatggcaca tggaaagcca tgcaggatgg
    11761 gaaaaatggt gagcgctatt tattggctaa cgtaaacctt agttataaag acttctttcg
    11821 aaaactttta aaacaaaata ataaaaaagc tttgctcgtt ccattgccaa aattcataat
    11881 gttattcttc ggaattgcag gagatattta ctcaatgctt ggtggtacaa cccaaatatc
    11941 actgacaaat tgcaaagcat tgtgtgtgaa aaatttctac tcaaactgca aagcacaaaa
    12001 acatttaggc atcgctttct ctccattagc attgggaata tcaaaaaatg agttccaaca
    12061 gaatccaaaa ataactctct caaaaaacga aacacataat tgcgcagaga ttgcttcgca
    12121 gtttgcaatg acaaacgaag aaaaatccta agccaatttc agagtcctca cttatttttt
    12181 tttcaaaggc atgagagact ctgaaattaa tacaatttac tttgcgcttg aaacactcga
    12241 aagaaactgc tttagtatcc tcattcgtta gtgttttttt atctgcaaga gtgactaaat
    12301 aaactaagaa ctaagaactc aaaactaaga actaaaaact aaaaactcag aactaaaaac
    12361 tcagaactaa aaactaagaa ctaaaaacta agaactaaaa cccttcctca cccccatcgg
    12421 cacatcttcc atttcaatag gaataagatt attgatatca caatgtgttt tgaaaccatt
    12481 aaaaataacc agcacttttt tatcgtccat actgtcaatc atgcctatgg ttttaccgcc
    12541 tttaagatga acaaatactc ccggcttcaa tttgcttata tactcttgct gtatacgttg
    12601 ttgttctttc gattgcactt tggctatttc gcttttctct ttgccatgca tgcgctcgta
    12661 ctttgccaac acggcttttt tatctttact gtttttccat tcgtttacaa attttatcac
    12721 cttatcgtca aactgtttta tcaaccgcaa ttccgtttgt tttattttcg aatcaattgt
    12781 ttgtttcgat tgttcagctt gcgatgatag cttgccgtac ttctccaaca tttcgtcaag
    12841 ggcttcttcg gcatgtgcaa tattctttgt ctttttatca atcacctgtt tttgattctc
    12901 cacatcgcgc aacatattct caagcaaaat atgatttttc gaaactttac tttcggcatt
    12961 ctttattatt tctttgtcca ttccactgcg ttcagccact aagaaagtat agctgctacc
    13021 cggcttgccc acaacgagct tatattttgg ctccaacttc tccggatcga aaagcataca
    13081 accgttaatg atgccttcgg ttttatcggc aagcactttc aggtttataa aatgtgtggt
    13141 gataatccct atcgatttca attcattcaa tttttctaaa atcgcttcgg ccaatgcacc
    13201 gcctagtgtt gggtcggtgc cggtaccaaa ttcatcaata ataaataatg tgcgttcatt
    13261 ggcttcgtgc aaaaacatat tcatcttttg caggcgcgat gagtatgtac tcaactcata
    13321 ttctatactt tgcgaatctc caatatcgac caacagctta tcgaaaaaac caaactcgct
    13381 atgtgcagcg caaggcacaa gaaaacccga ctgtaacatc aattgcagca gcgcaaccgc
    13441 tttcatacaa attgttttcc ctccggcatt tggcccgcta attaccataa tgcgattttt
    13501 atttcgcagg tacaatgaaa acggaatggt ggttttgccc tgcaatttat tttgcaacat
    13561 caacacagga tggtaagcat tctttatatc gatgcttggt tcatctttca acaaaggcaa
    13621 cacagcacca agttgcgctg catacatagc acgagcacgt agcaagtcgc attgcttttg
    13681 aatatcgaaa taggcatcaa tgtgaaaata ttttatacgg agttttgtgg taagctcttt
    13741 tagtattcgt aaaatttcgg tgcgctcttc ttgttccaaa ccaagtatag cattatttat
    13801 tggcagcagt tcatcgggtt ctaagtatgc cgtttttccc gtggcagaag tatcgtgaat
    13861 cactccaccg aggttacgct tttgttcagc aactatggca atcaccctgc gtccgttgcg
    13921 catactctcc tccgtatcgc ttatgtagcc ggccttacgg taacggttta caatggtttg
    13981 aaataatttg tcggcctcgc ttctttttcg cgaaaggctt tttcgtattt tatataactc
    14041 gtccgatgcc gatgatttta ttactccctc actgtcaaat acttcttcta tccacagctg
    14101 catactgtta tcgtaaagca agttgccaat tatttcttcg aacggctcat atattccttt
    14161 cctgtttttg aaaaaagaaa atatttgttg cgccacatgt aaacattgct gtatacgcag
    14221 gcattggtca gcactcaata tcgagttatc tattttcaac aaagcaagat cttttgatat
    14281 gtcggtgtaa tctctgtcgg gaaaattatc gccattatca atcatcattt taaattgatg
    14341 cacacgttgc aattcatttt gcatagcctc aaaatccgat gtgggcagca actgttgtat
    14401 cgcctcggca gtcacattca taaggctatg agtggccgct tgttgcttca cctgcaaaaa
    14461 ttcgttcagc aataaatttt gttgtggaaa aattaccata ccctgtaagc cttgcaaata
    14521 gttaaatata aagggcgcgc caacattctt atgttttgct tatgaacaaa tagtgttaat
    14581 aaatacaata tactcacttg tgtattaaaa atatctttgc gcaatattac attattctat
    14641 gtcaaataaa gaacaattaa ccgaactggt tacacaaaca agacgcgaca ttgtgcgcat
    14701 ggtgcatgca gtgcaatcgg gtcatcccgg tggctcgctg ggctgcaccg aatttatggt
    14761 tgcactctat aacactgtga tggagtacac ccccaaacca ttcaatatgg atggcaacaa
    14821 tgaagatatt ttcttcttat caaatggcca tataagtccg ctgttttaca gtgtgctggc
    14881 gcgtgcaggt tttttcgata taagcgaact cagtactttc cgaaaattaa attcccgttt
    14941 gcaggggcat cccaccacgc acgaacattt gcccggagtg cgcattgcga gcggctcgct
    15001 tgggcaaggc atatctgttg cagtgggtgc agcactggcc aaaaaaatga ataacgatac
    15061 ccataccgta tacgtactgt gtggcgatgg cgaattgcag gaagggcaaa tatgggaagc
    15121 tgtaatgttt gccgcagcaa aaaaagtgga taatattatt ctcaccatcg acctcaatgg
    15181 acaacaaata gatggcgcca caaaagatgt tttgcctata ggtgatgtgc gccaaaaatt
    15241 cgatgccttc ggttggcaaa catttcatat gacaaatggc aacaatattg acgaagtaat
    15301 cagcgtttta aatgatgcca aaagagcatg tggaaatgga aaacccgttg ccataattat
    15361 gaaaacagaa atgggtgccg gtgtcgattt tatgatgggc tcgcacaagt ggcatggcat
    15421 agcaccaaac gatgaacagc tgcaaaccgc attggcacaa ctacacagca ccattggcga
    15481 ctattaagaa attggaaaca cattgatgaa aaaaaatcag tttacaatta gttataaaag
    15541 tgtagaaaat aattttgtga aactttgtgc cttagtgcct tagtggcaaa aaaatttctc
    15601 gtcaaaacag aaaaaaaatg ctctcaaaaa actttgtgcc tttgtgtctt cgtggcaaaa
    15661 attcttaagc cttgaacagt aaaaaaaaca aacaatgaaa ttctccttcc catcgacatc
    15721 aaaagtattt tttgcaacag catttgcaat cgtcataacc atttttgcaa cacacaaatc
    15781 ctattcacaa aagcccacac ctgccccacc catcgaaacc cgcatattgt ttttatacga
    15841 ttgctcggca agcatgatgg cacaatggca aagcggaaat aaatacgatg tagccacaaa
    15901 attattatcg cacgtagtcg acagcttgca agatattccc aacctgcaaa ctgccctgcg
    15961 catgtatggc cacacaaaaa aattccctcc tcaagattgc gatgacaccc gcctcgaagt
    16021 acccttcgga tacaggaacg gttacaaaat aaaaggcaag ctcaaagaat ttaaacccag
    16081 cggcaccaca cccattgcct tatcgctcga ggcatgtggc aacgattttc ccaaagctgc
    16141 cggccgcaac attattatac tcattaccga tggcatagaa gaatgtaatg gcgacccttg
    16201 tgccgtttct tatgcattgc aacgcaaagg aatttcgctg cgccctttcg taattggctt
    16261 gggtgtgaac aaagaatttg caaaacaatt cgattgcata ggaacatatt acgatgccaa
    16321 agacgaagca tcattcaacg atatttttaa agtagtaata tcgcaagcac tcaacaatac
    16381 atcggcacaa ataaacttac tcgatattta taaaaaacct accgaaacaa atgtggccat
    16441 cactttttac gatatgctat cgggcaaggt gatgcaaaat ataattcaca ccatgaatac
    16501 aatgggcgtg cccgacacca taaaactcga tccactcaca acctaccgca tgcaggtaca
    16561 caccattcct cccgtgtata aagacagcat aaaactcacg cccggcaagc ataccatcat
    16621 aggtgtaagt gcaccacagg gcgacctccg cctcatactc gaagacagtt acgaatacaa
    16681 aaatctcaaa tgtatcattc gtaaacatgg cgaaatgaca acactcaatg tgcaaaatat
    16741 gaactccacc gagcgctata tcattggcga ttacgattta gaaattctta ccctgccacg
    16801 catcataaaa aacaatatcg aagtaaaaca aagtcacacc acatcggtcg aaattcctaa
    16861 acccggcctt gttaaccttg taagcagcaa ccccggcata ggcagcatac tggtagaccg
    16921 cagcggcacc tgggagtggg tgtgcgatat tgacgacacc acatcaaagc aaacactcac
    16981 attgcaaccc ggaaattatc gtttggtatt tcgacccaaa gcatcgcgcg aaacaattta
    17041 ctcactcgaa aaaaatttca acatcgtacc cggtggctct accactttgc aagtaaattg
    17101 atttgcgaaa atcgatgcgc aatatttttt gtgaatttat tttactccta actataaaat
    17161 caaaaataat tattggcgag cgggcggtgg cccttgcctt tgtgtcgcct gaggcgacac
    17221 aaaaataaaa tacccatttc tgcaaaggag aagtttaaaa gttcaagagt cccgacactt
    17281 cggtcgggat accgaccaaa gtgtcagtat ttaaatgttc aaaagttaat tccgcttatt
    17341 ttactttaga tgtttattat aatggttgta gtgatggttt gtatgttgta catttacaaa
    17401 cagaagccaa caggttgagt aagaagtttt ttaaagagta accacatgag ccaaaacttt
    17461 tttgtagaat gaataagtat atcataattg ttattatatt ttttttggtt agcgaaagtg
    17521 gggttgcgca aaatagaaat aacatttggt gttttggcaa tgatgccaaa ataacattta
    17581 ccaacggaac agcaattgcc gattcaacaa aatgcaatag tagaggcaca tgcgttagta
    17641 tatgcgactc attgggtaat ttactttttt atgcagcggg ttcaaccgat ggcctttcag
    17701 ctcaatatca ggttggtggt aaagcctaca acaaagaaaa ttctgtaatg caaaatggcg
    17761 atagcataat ggctcgtttg tggtataaag aaatggttat tctaccatac atttatagtg
    17821 atagcttgta ctatattgta agtgctcccg tcactggcaa aggtggtttt ttttactcaa
    17881 taatagatat gagccaagac agtggacgtg gcgcggtaat acaaaaaaat gtacaattgc
    17941 aaggctctaa tttttatgcc aatgatggca tcgcggccat caagcatggc aatggtagag
    18001 attggtgggt aatggttcgc gattggaagg atactaataa tgtttatttt ttttataagg
    18061 taactccaaa tggagtttcg ctttctcatt cgcaagcaat tggtgattcg ctgcggcccg
    18121 gtttttttag aatcgaacca tcggatgatg gtagtaaaat tgcatgttgt aatgcagggg
    18181 ggctaatttc tgtttttgat tttgataggt gtactggtta cttatccagt gaaatttcaa
    18241 tagaaaaaga atcaagtacg tatggcggtt tggcaccgtg gtactgggat tgcgaactat
    18301 cacccaacaa acgttactta tatgtgagtc aagaatttac cgattttgat accacaaatt
    18361 atttgctccg gttcgatttg caagcggcta acatacccgc aagccgagat accctttatg
    18421 caggcgaacg cagagcaact gcaggtttaa ttgaacttgc acccgatggc aaaatttatt
    18481 gggcatgtac ttactatcct cccggtacat ttttttatcc atacccagat agtacacata
    18541 attacataac agataattta agtgttatca attatgccga tagtagcttc tgcgattttc
    18601 agcctttctc tttttacctc ggtggtaagc gcacttatta tggtttgcct aataatccta
    18661 attatgggtt ggggcggttg ttggggagtc cttgtgatac gttgcagtgg gttggcacgc
    18721 ccgaaacccc caacccccta caagggggct taaatacaac atacatatcg gcttgggaga
    18781 agttgtttgt aaatgcacaa aacataaaag gaaaaaatgt aactataacc atatacgata
    18841 cacaaggtaa gctaatgtat gtagcgggaa agccccctaa agggggtttg ggggtttttg
    18901 gcgggtactt tactttggat gtagattgca atggttgggc aagtggattg tatgtggtac
    18961 atttacaaac tgagaaagaa atgttaagta ccaaatttgt gaaggagtaa aatatgaaac
    19021 aaaatatttt ggattatacc aaggaggttg gcggggacgc caaccgagga caaacactat
    19081 gagtaatggt tttgtaaata acacaagcaa accaactcaa atcagttggg ttacgaaaaa
    19141 atataatcat ttaaaaacat atttcccatt tgtatcaact gttacattta gttgccgctg
    19201 gctttgaaaa aatttatcgt accctatttt aatattttgc caaaagcgaa ttaatgccac
    19261 attggttttg ttttttaatt tatagttttc aaaattttta tcgtccattt taaaagggaa
    19321 aatataaacc ggtatttgtg tttgaccatt attatgtgca tacatggcat acaaataaat
    19381 ttcttttatt ttatcatcgg tcatgggcat gcaacctatt gtaacacaat tgccgtgaat
    19441 aaaaatatcg ccacccatat ttttagcctt gcttttaatt ttatcgctgg cattaggata
    19501 acttataccc agcgataagt agtaattact tgcagggtta tatctgttta tgtaataaaa
    19561 accttcgggc acttgattgt cgccatacgc acgcttggga ccaagttcac cactgccggc
    19621 acatatatcg taagtggcta tgagtgtata cgtagttgcc gttttcgatt tagcatatag
    19681 caacaatttc atttcggctt tgaaaacggt aatgagtaga ttaaaattat cggaagtaag
    19741 atgatgtgtt ttcaaatttt caaaaatcaa tttatgcttt gtttctatgg cattttgaac
    19801 ccgcgcatat ttcgattgtt ctttcaaaaa cgattgcgca tttgcataag tgaaaagccc
    19861 attcacgaga aaaattacag caagccattt ttcaatttgc ataaatctgc gaacaactat
    19921 tttcattaac cccatattgg aaatctattt caatgtatcg atgtcgtgta acaaaaaaca
    19981 ataatcatgt tttaccattc caatatgagg atagtaattg atagcagctg gtgcagcaag
    20041 taaaattatt ttggcttggg gtgctgccaa tttagtttgg cgtatgagtt ctttgccaat
    20101 gccttgtttt tgaaatgctt catctaccgc caaatcggct aagtaagtac aaaaagcaaa
    20161 atcggtgagt gagcgcgcta ctcccacaag cttgccattg ctgcgtgcag taacaataag
    20221 gttggcatgt tgcaacattt tttcgatgcg gtctctttcg tttaccggcc tgcgctcgcc
    20281 aagtgttgac acattgagta ccgtaataaa ttcatcggca tctaaatcgt tttcaatttt
    20341 atatgttatc attcttttct ttattaataa caacaaataa aaccaaaatt attgtgcacc
    20401 atataacaaa aacaacaatt ccatattcgg aaaattggaa taaatttgcg cttcgaaaat
    20461 aaaaatagaa caacaaaaat atgtttgaga atttatcgga taaactggac cgtgcgttca
    20521 aggtactgaa aggtcaggga aaaatcactg aaataaatgt tgccgaaaca ttgaaagaag
    20581 tgcgcaaagc ccttctcgat gccgatgtta actacaaagt agcaaaagat tttaccgaca
    20641 aagtaaaggc aagtgcattg ggccaaaatg tattgattgc cgtatcgccc gggcaattga
    20701 tggtgaaaat agtgaaagac gaactcacca gtttgatggg aggcgaacac agcgaactta
    20761 acttgaaagc ttcgccatgc atcatattga tgagtggttt acagggttcg ggtaaaacta
    20821 cattgagtgg caagctggcc aattttttga aaaataaacg ccaaaaaaat gtactgcttg
    20881 tagcatgcga tatttatcgc cctgcggcca tagaccaact cacggtgctt ggcgatcaaa
    20941 taggtgtaga ggtttacagc gaacgcgaaa ataaagatgc cgtaaaaatt gcacaaaatg
    21001 gtattgcacg tgcccgcgac cttggcaaga atgtggtgat tatagatacc gcaggccgcc
    21061 ttgccattga cgaacaaatg atggacgaaa tatcgcggtt gaagaatgca gtaaagccta
    21121 ccgaaacatt gtttgtagta gattctatga ccgggcagga tgctgtgaat actgccaaag
    21181 cctttaacga tcgcctcgat tttgatggcg tggtgcttac caagctcgat ggagatacac
    21241 gcggtggtgc tgccctttct ataaaatcgg tggtgaacaa gcccataaaa tttgtaggta
    21301 ctggtgagaa acttgacgag ctcgatatct tttatcccgc gcgtatggcc gatcgtatat
    21361 tgggcatggg tgatattgtt tcccttgtag aacgtgcaca ggaacagttc gatgagaagg
    21421 aagccatgga gttgcaaaag aaaatcgcaa aagataaatt caacttcaac gattttttac
    21481 agcagctgca gcagattaaa aaaatgggaa acatgaaaga cctagtaggc atgattcccg
    21541 gagttggcaa ggctttgaaa gatgtggaaa tagataacaa agcattcgat agaattgagg
    21601 ccatcataaa atcgatgaca ccaaaagagc gcgaaacacc tgaacttata aatattagtc
    21661 gtaagaaacg cattgccgca ggttgtggaa tggaaattgc cgaaataaat aagctgataa
    21721 aacagtttga cgaaacacgc aaaatgatga aggcattcac aacaggtaat atgaaaaaca
    21781 tgatgaataa aatgccgggg aggaggtagt tttctgtttt tagttcttag ttacgttggc
    21841 ggagaggatt gttcttcgtt cttcgtttcg tttgcggaga ggattgttct tcgttcttcg
    21901 tttcgtttgc ggagaggatt gttcttt
//
LOCUS       DAORQA010000021        21265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_638_length_21265_cov_14.940405, whole genome shotgun sequence.
ACCESSION   DAORQA010000021 DAORQA010000000
VERSION     DAORQA010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21265
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_638_length_21265_cov_14.940405"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1790
                     /gene="thrS"
                     /locus_tag="PK736_03045"
     CDS             <1..>1790
                     /gene="thrS"
                     /locus_tag="PK736_03045"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRC32402.1"
                     /translation="PDGAVKEYDNGTSAMEIAKSISEGLARNVLAAKVNGVVVEANRA
                     IDGDATLQLLTWDSLEAKATFWHSSAHMLAEALESFYPGVKFGIGPPIENGFYYDVDL
                     NGQSFTPEDFKKIEDKMLELAKQANKFERKEVSKADAIKYFTDKGDNYKLDLLQDLQD
                     GTISFYTQGNFTDLCRGPHIPDTGFIKAAKVMSIAGAYWRGDEKSKQLTRIYAITFPK
                     QKELTEYLTMLEEAKKRDHRKLGKELELFTFSEKVGMGLPLWLPKGTILRERLQSFLQ
                     KAQVAAGYQPVITPHIGHKDLYITSGHYEKYGADSFQPIKTPQEGEEFFLKPMNCPHH
                     CEIYKSKPRSYKDLPVRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHLFCRPDQVKE
                     EFCKVIDLVLYVFKALGFENYTAQVSLRDPENKTKYIGSDENWDKAEKAIQEAADEKG
                     LTTVVELGEAAFYGPKLDFMVRDAIGRKWQLGTIQVDYNLPERFQLEYTGSDNQKHRP
                     VMIHRAPFGSMERFVAVLIEHCGGNFPLWLAPEQIAVLPISDKYNEYGKKVLQNLNNC
                     DIRGFFDERDEKIGRKIRDAEMQKVPYMVI"
     assembly_gap    1791..1824
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2008..2598
                     /gene="infC"
                     /locus_tag="PK736_03050"
     CDS             2008..2598
                     /gene="infC"
                     /locus_tag="PK736_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRC32403.1"
                     /translation="MATQFRPGGSFPRKPFNPANRNRPRKENEHNINTDITATEVRLV
                     GENITVGVYSLQEATKMAEAQGLDLVEISPNAVPPVCRIVDYRKFLYDKKRKERETKA
                     NSAKSVMKEIRFGPQTDEHDFNFKVKHAQHFLEDGNKVKAYVHFKGRSIAYKEQGEIL
                     LLKFAEALQDYGKVELLPKLEGNKMFLHMIPISKKK"
     gene            2665..2859
                     /gene="rpmI"
                     /locus_tag="PK736_03055"
     CDS             2665..2859
                     /gene="rpmI"
                     /locus_tag="PK736_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRC32404.1"
                     /translation="MPKMKTHSGAKKRFKITGTGKIKRKKAFKSHILTKKETKRKRNL
                     THAGLVHKTDMNRVERMLTI"
     gene            2956..3297
                     /gene="rplT"
                     /locus_tag="PK736_03060"
                     /pseudo
     CDS             2956..3297
                     /gene="rplT"
                     /locus_tag="PK736_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993965.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
     gene            4065..4247
                     /locus_tag="PK736_03065"
     CDS             4065..4247
                     /locus_tag="PK736_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32405.1"
                     /translation="MKVAVKNKSEVEFVDELICGSKLLSTYNYTHTNDPFYEPYTFYN
                     ILCNNENTYYVFDHSS"
     gene            4311..4484
                     /locus_tag="PK736_03070"
     CDS             4311..4484
                     /locus_tag="PK736_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32406.1"
                     /translation="MKFTLNGVYDYYPYGKILREYVKGSKEKHLATHHQLDKKRPRLA
                     QCKIAMIGKAFTT"
     gene            4592..5824
                     /locus_tag="PK736_03075"
     CDS             4592..5824
                     /locus_tag="PK736_03075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC32407.1"
                     /translation="MRSLFSAYKNSFSNIQRNIWVLAISMFINRSGSMVLLFASLYFT
                     HDLHFSFAQAGVVMSFYGMGSICGSYLGGWLTDRKNLGHIMMASLAGSGIILLCLLLT
                     SNPVLISVIIFFYALVADIFRPANSKATAQYSDPDNLTRSVSLIRLAINLGFSVGPAA
                     GGFIALYFGYKWLFAIDSVTSIGAAVMLWFYLPRIPHEKKSSAIEKQKPKSLSAYKDV
                     SYLLFIILVSLYAICFFQIFASVPQYFSKECNYSSNTIGLLLALNGFLVVVIEMPFIT
                     MLEKNKRIFRYIIMGTLCLPVCFSMLLFGHNLMVAAIFYTVIITFSEIFAMPFMMKYV
                     LSKPEADRQGQYAALYSIAYGIANICAPLLGLGIADRYGFEVMFYFFIVVSICVAISF
                     YFLAMRNRKILLTASLES"
     gene            6017..6367
                     /locus_tag="PK736_03080"
     CDS             6017..6367
                     /locus_tag="PK736_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HRC32408.1"
                     /translation="MLKVYFKNNCSTCRTALAMMKDNTKEKVETVEYLVDTPSEEEIK
                     DILKMLGMKAFQLVRAKESLYHEKYAGKKISNAQWIKILHRNPILIERPIIVKEGKAI
                     IGRPIERIIEFIGK"
     gene            6446..6838
                     /locus_tag="PK736_03085"
     CDS             6446..6838
                     /locus_tag="PK736_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRC32409.1"
                     /translation="MEYKPIEPELEDIGKIIVHSSYLVHKSLGPGLLEKVYEICLCHE
                     LIKAGLIVKRQIDIPIVYDGLTFNEGLRLDILVEDKVIIETKAIDLINPVWEAQVLSH
                     LKITGLRLGYLINFNIVNIGQGIKRFVK"
     gene            7040..7420
                     /locus_tag="PK736_03090"
     CDS             7040..7420
                     /locus_tag="PK736_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRC32410.1"
                     /translation="MKDYKKYFMLNATPEEVYIAITNPLTISLWTNEEATMSTEPGSE
                     FSIMDGAIEGRNIEFVENKKIVQQWYFGEQEEPSIVTIKLHWHASGTSVELMHKNIPD
                     EAYDDIVDGWNEIYFGNLEEFFNE"
     gene            complement(7614..9692)
                     /locus_tag="PK736_03095"
     CDS             complement(7614..9692)
                     /locus_tag="PK736_03095"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRC32411.1"
                     /translation="MPKNLLIYVSILIFCCTNIYAQTFSGTGDTIPDDGTPIEFVINV
                     SGIPDTIDITNFGLEKVCFDLLHTYDADMRIHLIAPDGTIILLLDGCGGGDDNFINTC
                     LNDQSNVSILTGTAPFTGLYKPIGQIGMVNNFQNPNGIWKLRILDTYAFADFGILNSW
                     EIDFSNTPSVPTFFDSTFLPMVQINTYGQFILDDPKINAAMTWIDNGVGNYNHRTDSA
                     NVYNGFIGIEIRGHSSQMFPQKSYSIELRDTFGNAIDTPLAGFPNESDFILHGPYTDK
                     SLMRNVLTCDLANKMGRYSSRTKYVELIVNDEYQGIYVLMENIKRGKDRVDIAKLDND
                     DLAGDSLTGGYICKFDWGPGLQWNSNYPPDPVNVDNNIVEILMHYPKDGNIMPQQFQY
                     IKNYVDSFETALYSTYSADLNIGWRKYGEEYSFIDFLLLNELSKNVDGYRLSSFFYKK
                     KDSDGGKLHMGPVWDFNLAWHNADYCNNQFTAGWAYRITDYCAWDMPAWWKKFVTDPN
                     FITRTRCRWNDLRQNVLDTNNLFFYIDSVSNYLSIPAQSHYDRWPILGVYVWPNPNPL
                     AQTYNQEIASTKKWITQRIAWLDANIPGTCTSAGVIANQNKDNNFKCFPSPTVDELQI
                     IHSGFDATNCVLNIMDDKGSIVKTIAHNFIPFNTQQISIDVKNISSGIYAVLITNTEG
                     ETKCLKFMKQ"
     gene            complement(9749..11008)
                     /locus_tag="PK736_03100"
     CDS             complement(9749..11008)
                     /locus_tag="PK736_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC32412.1"
                     /translation="MQKKHQATSNPLNIIVIVAALGYFVDIYDLILFSITRIDSLRAV
                     GITEQAQLTSTGEHLLNMQMIGMLVGGIIWGIMGDKRGRVSVLFGSIIMYSFANIANA
                     YFIELSNYFNVDAVQMYGWLRLIAGIGLAGELGAGITLVNETMTKESRGYGTMIVVSF
                     GVLGAVAANRVSLFGWQNAYIIGGVMGFLLLLMRIGVYESGMFDALREKNLVKGDFRK
                     LFNNRKRFISFISCIGIGLPIWFMIGILTSFADVYCKANNIVGIKPKDAVMYFYIGTS
                     FGDFLSGYLSQVFKSRKAVLFGYLVFATILIPVYLFSQNLSPLAFYILSALLGLAAGY
                     WAVFVTIASEQFGTDIRSIVTTTVPNFVRGGLVLMATTNIFLRDGLQLDVRVAALCVG
                     LASLFIAFISLRGMHETYGKELAYVEE"
     gene            complement(11547..12473)
                     /locus_tag="PK736_03105"
     CDS             complement(11547..12473)
                     /locus_tag="PK736_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRC32413.1"
                     /translation="MPINQYEIQHISHLELLARQVVEGFITGLHKSPFHGFSVEFAEH
                     RLYNTGEPTRHIDWKLYGKTDKLFVKRYEEETNLRCHIIVDNSGSMHYPEVDSRNPNL
                     LNKFTFSAYSAAAIMYLLKMQRDAVGLSLFSNDVEVNTPAKSTTVHHKLLYQYLDTAI
                     AKGTTNNNKTSAAAALHQIADSIHKRSLVIIFSDMFENMEQQEELFGALQHLKYNKHE
                     VVLFHVVDKSKEIDFNFENRPYEFIDIESGERVKVHSNQVRQQYLESMAAFKKELNIK
                     CAQYKIDIVEADIHKGYQQVLMPYLIKREKMN"
     gene            complement(12670..12990)
                     /gene="trxA"
                     /locus_tag="PK736_03110"
     CDS             complement(12670..12990)
                     /gene="trxA"
                     /locus_tag="PK736_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRC32414.1"
                     /translation="MALELTDANFEELVLKSNVPVLVDFWAEWCGPCRMVGPFVEELA
                     VEYDGKAVVGKVNVDHNPNVSMQFGIRNIPALLIFKDGKIVDKQIGAAPKATLAQKLD
                     AQLA"
     gene            complement(13086..15353)
                     /locus_tag="PK736_03115"
     CDS             complement(13086..15353)
                     /locus_tag="PK736_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRC32415.1"
                     /translation="MDKLVRQRVRRFVFSFPIQLVVVQAKKNHFQLLYWLLLFCFVGE
                     LLMARNGVPYLFFDPEYAGRVNYFSFFLTGICLGVFIIAYNISSYLLNSHRFPFLASL
                     SRTFFKYSLNNCIIPVAFIFFYIFKIAQFQYNAQSISALHIILLQCSLLGGMILILSL
                     SFWYFSLTDKSIYKIYGIGPATDDNDDMVAVSTLFTRKKIVEQMRLKKWIVYVEVYLA
                     SFSKIKLVRDTSHYDQEKLKFVLRKNHYNAAIFQSAVLVVFLSLGFLRDVDVFQIPAA
                     ASIFLLFTLFIMLSGLFSFWLGSWVTTMAIAILLFFNWVSGFEFVNQRNQLYGVNYDS
                     APVNYNLQLTDSLCDAKFVNDDIKSTISILENWKKKNTIDTASKPKLILLNVSGGGTR
                     SMVFTFETLQRIDSVLNGQLMNNTMMIAGSSGGMIGAAYYRQLFYNRTVLQKQPYPFI
                     NKNDKINVGKDLLNTTIFSLTVNDLFFSLQKFDDGKHKYIKDRAYAWERQLNKNTENV
                     LQHRLKDYLMPEQNAQIPMLMLSSTIVNDGRVLYITPQPVSYLLQTGKAPGINTQILT
                     NGIEFTKMFANHDALDLQFSSALRMNATFPYIMPPASLPSIPPMECMDSGIRDNFGLH
                     TTCRFLQVFKKWISDNTSGVVIIQIRDTYKHAAFRDNSAKTLIQNLFAPLRNVSNNLI
                     IMQEYNNDAYLQYVLDLYNQNINLVTFELPESELHVPLSWHLTSTEKTYIEQQTINKD
                     NAQSLQYLQKVMLNK"
     gene            15594..15935
                     /locus_tag="PK736_03120"
     CDS             15594..15935
                     /locus_tag="PK736_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HRC32416.1"
                     /translation="MAYNEELADRIRERLAELPNIEEKKMMGGLTFMVNDKMCVGIIK
                     DELMCRIDPEFHETAVEKNGCRTMDFTKRPMIGYVLIDDTGMRTQREFDYWINLALDF
                     NAKAKSSKKKK"
     gene            complement(16064..16423)
                     /locus_tag="PK736_03125"
     CDS             complement(16064..16423)
                     /locus_tag="PK736_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32417.1"
                     /translation="MTKYKAINLSPVIPSTDVALTTNFFYDVLGFSIFRNYGYYSIVF
                     NDAFSVHISEAKSECTSVSIYLEVDDIELFWNTVKGKIDMANSKPPFDQPYEMREAIV
                     SIPNTKSKIIAGQSVIK"
     gene            complement(16703..18091)
                     /locus_tag="PK736_03130"
     CDS             complement(16703..18091)
                     /locus_tag="PK736_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HRC32418.1"
                     /translation="MKKLTLILMLFTSIAYAQYPSQNVTLLSTWDDITVPAESYYGIR
                     YNGIWGWTSPNGMEYAIIGATTGTYIINVSNPATPVVCDFVPGCRVDCIWRELKTYQN
                     FLFMVSDDSPPNCLQVADLSYLPDSVHVVHESDNIIETAHTIFIDDHYLYAASPRGGV
                     VGNSASMAVYDIINPATPVLLRKLNQDDPSVGGVHDMLVKNDTVYASAGFDGLHIYLY
                     DTGANVFTIYNSVTVYPGSGYNHSTAITTDSKTLVVADEVPTGLPLKTFDITDFSNIT
                     IPTTFRSTQASTATPHNPFMMKDNYCVVSYYQDGVQIFDVSNPATPVRTGYIDTNPTD
                     GLGLPNPSYSGSWGSYVEFPSGNIVASDMQNGLFVIDPTMSLGITSAASNSNNLIVYP
                     NPVQNFLQLHFSTAAIYNVELMDITGKTIFSKVTNGSQVVNFDMGNINKGIYIIKASN
                     DNTSFVKQVVKQ"
     gene            complement(18122..18931)
                     /locus_tag="PK736_03135"
     CDS             complement(18122..18931)
                     /locus_tag="PK736_03135"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRC32419.1"
                     /translation="MLEKIKQTAEFIKSKTSFTPKYGIVLGSGLGGLVTKIKSECEIS
                     YSDIPNFPVSTVHGHAGKLMFGHLGNTPVLVMQGRFHFYEGWTMEQVVFPIRVMKYLG
                     IDNLILSNAAGGMNKNFQVGDLMIIEDHINLMPSNPLIGKNDDNLGPRFPDMSVPYNK
                     EMIARAKVIADEMGHRCHIGVYAAVSGPCFETPAEYKYLRIIGADVVGMSTVPEVIAA
                     KHCGMNCFAMSIVTDLGGFDDVQSVSHEEVLKVANEAEVKMSAIVAQLLNS"
     gene            complement(18915..19955)
                     /gene="lpxK"
                     /locus_tag="PK736_03140"
     CDS             complement(18915..19955)
                     /gene="lpxK"
                     /locus_tag="PK736_03140"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRC32420.1"
                     /translation="MRILLFPFALIYGAVVWLRNKLFDFKIIESIQFPFPVIIVGNLQ
                     AGGTGKTPMVKYIANLLKQEKLCAILSRGYKRKTSGFLFATNSDNSSTIGDEPYEYFK
                     LYNNAVSVAVCEKRVDGIEQLQKIKKHDVVICDDAFQHRYLKSEMDIVLTAYGDLYCN
                     DWLLPAGNLREPISGASRAQIIIVTKCPDDLSIEAQKNIAQQIKINPHQHLFFTTLIY
                     TTLYNPFSNQSFNQAAAGTHMALLVSGIAKPSSLQKYIGEQFAKTELLTFADHHEYTY
                     NDLVTINEKFNTLATENKILITTAKDFARLSTMKLPFEKEKLFIQNIEVGFLNNQKSK
                     FDMLMQNYVRKN"
     gene            complement(19980..>21265)
                     /locus_tag="PK736_03145"
     CDS             complement(19980..>21265)
                     /locus_tag="PK736_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC32421.1"
                     /translation="FYVLLPLPILVFIIYKVHDIINKKSDKVQSQLSTMSTFVQESFS
                     GIRVLKAFAREKEFENAFDKQCADYKNASMELARVNAFFFPSITLLIGVSSIITIYVG
                     GLAVINNQLTVGNIAEFVIYVNMLTWPVASLGWIITLIQRAAASQQRINEFLSIPISI
                     KSSTNDDKTIDGKIAFNNVSFTYSNSGITALRNISFVARPGECIAFIGKTGSGKSTIA
                     NLLLRMYDTANGEILIDDKNITQHNLQNIREQIGYVPQEVFLFSDTIKHNILFGIKDT
                     TNEKSLHEKMLASAHDAAIYDNIINFNDGFETMLGERGINLSGGQKQRVSIARAIVKE
                     PRILIFDDCLSAVDTETEETILQNLQRIMKDKTTVIISHRVSSVKTADTIYVLDDGKI
                     MEHGSHHQLIEANGIYKTMYDTQTNREKLQAQLAE"
BASE COUNT         6820 a         3847 c         4017 g         6547 t
ORIGIN      
        1 taccggacgg agcagttaag gaatatgaca atggcacatc tgccatggaa attgctaagt
       61 cgataagcga gggccttgcc cgcaatgtgc ttgcagccaa agtaaacgga gtagtggtag
      121 aagctaaccg tgccattgat ggtgacgcaa cattgcaatt gcttacctgg gacagcctgg
      181 aggcgaaagc caccttttgg cattcatcgg cacatatgtt agccgaggca ttagaaagtt
      241 tttatccggg agtaaaattt ggaattggcc ctcctataga aaatggtttt tattacgatg
      301 tagatttgaa tggtcaatcg tttacacccg aagattttaa aaagatagaa gataaaatgc
      361 ttgaattggc aaagcaagcc aataagtttg agcgtaaaga agtatcgaag gccgatgcga
      421 taaaatattt tactgacaag ggcgataatt acaaactcga tttgcttcag gatttacaag
      481 acggaacaat atcattttat acacagggaa actttaccga tttatgccgt ggccctcata
      541 tacccgatac cggttttatt aaggcagcaa aggtaatgtc aatagccggt gcatattggc
      601 gtggcgatga aaaaagtaaa cagcttacaa gaatatacgc cattactttt ccaaagcaaa
      661 aggaacttac cgagtacctc accatgctcg aagaagccaa gaagcgcgac caccgaaagc
      721 ttggtaaaga gttggagtta ttcacgtttt cggaaaaggt aggaatgggc cttccactgt
      781 ggttgccaaa aggaactatt ttgcgcgagc ggttacaatc atttttgcag aaagcacaag
      841 tggcagcagg ctatcaacct gtaatcactc cgcatattgg tcacaaggat ttatacatca
      901 catcgggcca ctacgaaaag tatggtgccg attcgtttca accaataaaa acaccacaag
      961 aaggcgagga gttttttcta aagccaatga actgcccgca ccattgcgaa atatataaaa
     1021 gtaaacctcg ctcttataag gatttaccgg tgcgtcttgc cgagtttgga accgtatacc
     1081 gttacgagca aagtggtgag ttgcatggcc ttacgcgtgt gcgtggattt acgcaagatg
     1141 atgcccattt attttgccgc cccgatcagg ttaaagaaga attttgtaaa gttatagatt
     1201 tggttcttta tgttttcaaa gccttaggtt tcgaaaacta tactgcacaa gtttcgcttc
     1261 gcgatcccga aaacaaaacc aaatatattg gcagcgatga aaattgggac aaagccgaaa
     1321 aagccataca ggaagcagcc gatgaaaaag gactgacaac agttgtggaa cttggcgaag
     1381 cggcctttta tggcccaaag ctcgatttta tggtgcgcga tgccattggt agaaagtggc
     1441 aattgggaac aattcaagtt gattataatt tgcccgagcg ctttcaactg gaatatacag
     1501 gtagcgataa tcaaaagcac aggccggtga tgatacaccg tgcgccattc ggatcgatgg
     1561 aacgttttgt ggcggtattg atagaacatt gtggaggtaa tttcccgtta tggctggcac
     1621 cggagcagat tgctgtgttg ccaataagcg ataaatacaa cgagtatgga aaaaaagttt
     1681 tacaaaatct aaataattgc gatattcgcg gcttctttga cgaacgtgac gaaaaaatag
     1741 gacgtaagat acgcgatgcc gagatgcaaa aagtgcctta tatggttatt nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnggcgaa aaggaagcag ccgaaaatgg catttcgctt
     1861 cgcaaacacg ggcaaggaga tttaggtttc tttacaatgg aagagggcat taagctattg
     1921 atgagcgaaa taaataagga tttagaaaaa gccgcaagtg cttgatagat tttaaatatt
     1981 gaatattaat tttaaaaaga aagggttata gcaacacaat tcagaccagg aggaagtttt
     2041 ccacgtaagc catttaatcc ggcaaacagg aatcgtccgc gtaaagagaa cgaacacaat
     2101 atcaataccg atattaccgc aacggaggta cgattggtgg gcgaaaatat aacggtgggt
     2161 gtatactcat tgcaagaagc caccaaaatg gctgaagctc agggtttgga cttggtagaa
     2221 atatcaccta atgcggttcc gcctgtttgt agaatagttg attatagaaa atttctttac
     2281 gataaaaaac gtaaagagcg cgaaaccaaa gccaactcag caaagagtgt gatgaaagaa
     2341 attcgctttg ggccgcagac agatgagcat gattttaatt ttaaagtaaa gcatgcacaa
     2401 cactttctcg aagatggaaa caaggtaaaa gcctacgttc attttaaagg ccgttcgata
     2461 gcatacaagg agcagggcga aattttgttg ttgaaatttg ccgaggcttt gcaggattat
     2521 ggcaaagtgg aattattgcc taagttggaa ggaaacaaaa tgtttctgca catgattcca
     2581 atatcaaaga agaaataaaa gtataagtaa gtataacaag ttccgaaata atagaaaatc
     2641 ggaaaaatta aaaacaaagt agtcatgcca aagatgaaga ctcactcggg tgccaaaaaa
     2701 aggtttaaaa taaccggtac cggaaaaata aaaagaaaaa aggcttttaa aagccacatt
     2761 ctaaccaaga aggaaacaaa gcgcaaacgc aatttgacgc atgcaggctt ggtacacaaa
     2821 acagatatga atcgtgtaga aagaatgttg accatctaat ttcaaaaaag aatttcaaat
     2881 aagtttaacc aggactgtta gcttaaaagt tgcatcaaaa aaagttgcac gctacatcca
     2941 aaaagcaatt aaaaaatgcc acgttcagta aacagcgtag cgtcaagagc aagacgcaaa
     3001 aaaatgttca aactcgcaaa aggctttttt ggctcgcgcg gaaatgtgct cacagtagcc
     3061 aaaaacactg tagaaaaggg tttgcaatat gcttaccgcg accgtaaaac caaaaagcgc
     3121 accatacgcg gactttggat acaacgtata aatgccggag tacgcgaaca cggcatgtcg
     3181 tattccactt ttataggcaa gtgccatgca ggcggtatcg accttagccg caaagtatta
     3241 gccgacttgg caatgaacca tcctgatgct tttaaagcca tcattgagca agtaaaaaat
     3301 cttaccccta cacgttcaaa agattctact tacgaaggca aacaagtagc cctggctccg
     3361 gcatcgcgtt ctgcaaaacc acttaataag gttgaagcaa agacaaaaaa tgctgtagtt
     3421 gaagcacctg ctgccccaaa agctaaacct gctaaagttg caaaacctgc cgcagcgcct
     3481 gcccccgatt tatttgccgc agctgcggtt gaaacggaag cccctgccgc agtagaagcc
     3541 gttggaccaa aagatgattt gaaaaaaatg gaaggcgttg gacctgcaat tgaaaaactt
     3601 cttaatgctg ccggtataaa cacctgggct cagttagctg aaaccgaagt agaaaagctt
     3661 gaagctatat taagcgaagc gggaagccgt tttacaacac ataagccagg cacatggcca
     3721 gcacaagcga agttggcagc cgaaggaaaa tgggatgaac taaaagcttg gcaagaccaa
     3781 ttagacggag gtaaagagta atctttatat aattatcaat aacaaaaaag gctgcaacac
     3841 atgttgtggc cttttttggt taatgcggtt cttaggtcct ttttcaaatt accataaggg
     3901 caagttatgc tattggtaat tttaattgca taattcgggt ttaataaaag tgaattatgt
     3961 taagttgcaa aagggcccaa tttaaaaaga ttggttttct tcaaccaaca aacctcaaca
     4021 caggcactca acacagacta atttattttt ctattttcga aaaaatgaaa gttgctgtaa
     4081 aaaataaaag tgaagtagag tttgttgatg aacttatttg cggtagcaag ctgttatcta
     4141 cttacaacta cacccacact aacgacccat tttacgagcc atatactttt tacaacattc
     4201 tttgtaataa tgaaaatacc tactatgtat tcgaccacag ttcgtgaata ctcactgcaa
     4261 gtcttgccaa cgcgcgtata acgtatacac ccacagttat gcccgatggt atgaaattta
     4321 ctctaaatgg tgtatacgat tactaccctt acggaaaaat actgcgcgag tatgtgaagg
     4381 gctcaaaaga aaagcacctc gccacacatc atcagctgga caaaaaaagg cctcggttag
     4441 cgcaatgtaa gatagcgatg attggcaaag cttttaccac gtaattgtac aacaattctg
     4501 cttagggccg atttgaattt acgcagaaaa taaattcgct gttcgattta atataaattt
     4561 atacacttta tttacaatcg aaattaaaag catgagaagt ttattttctg catacaaaaa
     4621 ttctttttca aatattcaac gcaatatttg ggtgcttgcc atttccatgt ttatcaaccg
     4681 cagtggctcc atggttttgc tttttgcttc gttatatttt acccacgatt tgcattttag
     4741 ttttgctcag gcaggtgtgg ttatgagttt ttatggcatg ggaagtatat gtggttcgta
     4801 ccttggtggt tggcttaccg acaggaaaaa ccttggacat attatgatgg catcgcttgc
     4861 cggtagtggt ataatattac tttgcctatt gcttacgagc aatccagttt tgataagtgt
     4921 tatcatattt ttttatgcac tggttgccga tatatttcgc cctgcaaatt cgaaagctac
     4981 agcacaatat agcgaccccg ataatttgac ccgctcagta tcattaatcc ggttggctat
     5041 aaaccttggt ttttcggtgg ggcctgctgc aggaggattt atagcacttt actttggcta
     5101 caaatggttg tttgcaattg attctgttac cagcattggt gcagcggtaa tgttatggtt
     5161 ttatttacca cgtataccac acgaaaaaaa atcttcagct atcgaaaagc aaaaaccaaa
     5221 atcgctttcg gcttacaaag atgtttcata cttgcttttt attattttgg tttcgttata
     5281 tgccatttgt ttctttcaaa tctttgccag tgtgccgcaa tatttttcta aagaatgtaa
     5341 ttactcatca aacacaatag gtttgttgct tgccttaaat ggttttcttg ttgtggtaat
     5401 cgaaatgcca ttcattacca tgctcgaaaa aaataagaga atatttcgtt atataattat
     5461 ggggacattg tgtcttcctg tgtgttttag catgctgctg tttggccaca atttgatggt
     5521 agcagcaatt ttttatacag tgataattac cttctccgag atatttgcca tgccatttat
     5581 gatgaagtat gtactatcga aacccgaagc tgaccgccag ggtcaatatg ccgcattgta
     5641 ttctattgca tatggtattg caaatatctg cgcaccattg ctcggcttgg gcattgccga
     5701 cagatatggt tttgaagtga tgttttattt ttttattgtt gtgagcattt gtgttgcaat
     5761 aagcttttat tttcttgcta tgcgaaacag gaaaatactt ttaacagcaa gtttagaaag
     5821 ttagaaagga gtttaggaag tttgtaggtt gtgaaggtta agaaagttag aaatgagatt
     5881 ggaaagttac agatatttta tacattgatt tttatgattc aatatcttcg tttatcatca
     5941 caatcataac tatctgcttt cattcctaaa taagctttgg tttttttatc aacttcgcgc
     6001 acttagaatt tacataatgc tgaaagtata tttcaaaaat aattgttcta catgtcgtac
     6061 cgcattggca atgatgaaag acaatacaaa agagaaagtt gaaaccgtgg agtatcttgt
     6121 tgatacacca agcgaagaag aaataaaaga cattttgaag atgttgggta tgaaggcatt
     6181 tcaacttgtg cgtgcaaaag aatcattgta tcatgaaaaa tatgccggca aaaaaatatc
     6241 taatgcacaa tggataaaaa ttttgcaccg taatccaatc ctgattgaaa gacccattat
     6301 tgtgaaagaa ggcaaggcta ttatcggccg gccaatagaa cgaattattg aatttattgg
     6361 taagtgatat ttaactcaaa tcaaactatt gtagaatata gaataaatct tgccactaaa
     6421 tcactaaaac acaaaatccc tcaaaatgga atacaaacct attgaacctg aactggaaga
     6481 tattggaaaa ataatagttc attcatctta tcttgtacat aaatcgcttg gaccaggttt
     6541 attagaaaaa gtttatgaga tatgcctttg ccatgaatta atcaaggcag gtttaatcgt
     6601 taaaaggcaa attgatattc cgattgtata tgatgggttg acttttaatg aaggattgcg
     6661 gcttgatatt ttggtggagg ataaagtaat aattgaaaca aaggccattg atttaataaa
     6721 tccagtatgg gaggcacagg tacttagtca tttaaaaata acaggattgc gattgggtta
     6781 tttaattaat tttaatattg tgaatattgg gcaaggtatc aagcgttttg ttaaatagta
     6841 tgaatttagt gttgccgaaa tatgataatg agcttgtttt ttttaattat aaatacacat
     6901 cactatagtg tttaaaaccc cttagaaaat ttttgtggga tttagtgact ttgagcttta
     6961 gtggcagaaa aagcgcaaat tgttttttta ttttaagtga cataaagttt tttgcaaaaa
     7021 aataaacaca ataaaaaaaa tgaaggacta caaaaaatat tttatgctga atgccacacc
     7081 ggaagaagtg tacatcgcaa taacaaatcc attaacgata tcgttgtgga caaacgagga
     7141 agcaactatg agcaccgaac caggcagcga gttttcgata atggatggtg ctattgaagg
     7201 tcgcaacata gagtttgtgg agaataaaaa gatagtgcaa caatggtatt ttggtgagca
     7261 ggaagaacca tcgattgtaa caataaaatt acactggcat gcctcgggca catcagttga
     7321 actgatgcac aaaaatattc ctgacgaagc ttatgacgat attgtagatg ggtggaatga
     7381 aatctatttt ggcaaccttg aagagttttt taacgaataa aatttgtgat aggtcaatca
     7441 aaaatatttt tgccacaaag gcacgaaggc acaaagtttc acaaaattat ttgataccga
     7501 ttttttattt ctgatttgtg atttttttat ttcaagcact tctgtcgtgt tgaacatttt
     7561 ttttctcagc gcatgcagca gaataaattt taacccacat tttttaaaaa aaattattgc
     7621 ttcataaatt tcaggcattt tgtttcgcct tctgtattgg ttattaatac agcataaatt
     7681 cctgacgata tattttttac atcaatagaa atttgttgcg tattgaaagg gataaaatta
     7741 tgagctatcg tttttactat gctgccttta tcatccataa tattcagcac gcaattggtg
     7801 gcatcgaaac cggaatgaat tatttgcaat tcatcaacag taggagaggg aaagcactta
     7861 aaattattgt ccttattttg gttcgcaatt acacctgccg aagtacaagt tcccggaata
     7921 ttggcatcga gccaggcaat acgttgagta atccattttt ttgtactcgc tatttcttga
     7981 ttataagttt gtgccaaagg attagggttt ggccacacgt atacaccaag tataggccat
     8041 cgatcataat gactttgtgc aggaatgctt aagtaatttg ataccgaatc gatatagaaa
     8101 aataaattat ttgtatcgag tacattctgt cgtaaatcgt tccaacggca acgtgtgcgg
     8161 gtaatgaaat ttggatctgt tacaaatttt ttccaccatg caggcatatc ccatgcgcaa
     8221 taatcggtaa tgcgataagc ccatccggct gtaaattgat tgttgcagta atcggcattg
     8281 tgccaggcaa gattaaaatc ccaaacaggg cccatgtgta attttcctcc atcgctgtct
     8341 ttctttttgt agaaaaaaga actcaatcta tagccatcta catttttcga taactcgttt
     8401 agcaaaagaa aatcaatgaa agaatattcc tcaccatact tacgccatcc aatatttaaa
     8461 tcggcactgt aagttgaata caatgcagtt tcaaaactat caacataatt ttttatgtac
     8521 tgaaactgtt gtggcataat attgccatcc ttgggatagt gcatcaatat ttctacaata
     8581 ttattatcca catttaccgg gtcgggtggg tagttcgaat tccattgcaa gcccggtccc
     8641 caatcgaatt tgcaaatgta tccacctgta agcgaatcgc ctgcaagatc atcgttgtcg
     8701 agtttggcaa tgtcaacgcg gtctttacct cgttttatat tttccatcaa tacataaatt
     8761 ccctgatact catcattcac aattaactca acatattttg tacgacttga ataccgtccc
     8821 atcttatttg caaggtcgca cgtaagcaca ttacgcataa gcgatttatc ggtgtatggc
     8881 ccatgtaaaa taaaatcgct ttcgttaggg aaacctgcca gcggagtatc aatagcattt
     8941 ccaaaggtat cgcgcaattc tattgagtag gatttttgcg gaaacatttg tgaactgtgc
     9001 ccacgtattt ctatccctat aaatccatta taaacattgg ccgaatctgt tctgtgattg
     9061 taatttccaa caccattgtc aatccatgtc atggctgcat ttattttagg gtcgtcaaga
     9121 ataaattgcc cataagtatt tatttgcacc atgggcaaaa aagtagaatc aaaaaaagtt
     9181 ggtaccgaag gtgtattgct aaaatctatt tcccacgaat taagaattcc aaagtcggca
     9241 aacgcataag tatcaagaat tcttaatttc caaattccat tagggttttg aaaattattt
     9301 accatgccta tttggcctat tggtttatat aagcctgtaa agggcgcagt gccagtaagt
     9361 atagaaacat tagattgatc gtttaaacag gtattaataa aattatcatc accgccacca
     9421 catccatcca gcaataaaat aatggtgccg tcaggtgcaa tgagatgtat gcgcatatcc
     9481 gcatcgtaag tgtgcaacaa atcgaaacaa actttttcta atccgaaatt tgtaatgtcg
     9541 atagtatcgg gtatgccgga aacatttatt acaaactcaa tgggcgtgcc atcatcgggt
     9601 atggtatcgc ctgtgccgct gaatgtttgc gcataaatgt ttgtgcagca aaaaatcaat
     9661 atcgaaacat aaatcaaaag gttttttggc atctgcattt taaagttaat tattggtatt
     9721 atgtttttta taatctgtat tcatgttttt attcttcaac ataagctaac tctttgccat
     9781 aggtttcgtg catgcctcgt aaactaatga aagcaataaa taaagaagca aggccaacac
     9841 acaaggctgc aacacgcaca tcgagttgaa gaccatcgcg caaaaatata ttagtagttg
     9901 ccatcaatac taaacctcca cgtacaaaat tcggaacagt ggtagttact atactacgta
     9961 tatccgtacc gaattgttcg ctggcaatag ttacaaacac agcccagtat cctgcggcca
    10021 gaccaagcaa tgccgacaga atataaaatg ccaggggaga taaattttgc gaaaataaat
    10081 atacgggtat aagtatggtt gcaaaaacta aatatccaaa aagcactgct ttacgtgact
    10141 taaaaacctg acttaaatat ccgctcaaga aatcgccaaa cgatgtgcct atgtaaaaat
    10201 acatcacggc atctttaggt tttataccaa ctatattatt agccttacaa taaacatcgg
    10261 caaaagatgt gagtatgcca atcataaacc aaataggaag ccctatacca atgcaactaa
    10321 taaaagaaat aaatcgcttg cggttattaa acaatttacg gaagtcgccc ttcactagat
    10381 ttttttcgcg cagtgcatca aacattcccg attcgtatac accaatacgc atgagcaata
    10441 gtaaaaatcc cataacacca cctatgatgt atgcattttg ccaaccgaat aaactaacac
    10501 gattggcagc tactgcacca agcacaccaa acgaaactac tatcatagta ccataaccac
    10561 gactttcttt tgtcatggtt tcgtttacca atgtaatgcc tgcacccaat tcaccggcaa
    10621 gaccaatgcc tgcaatcaaa cgtaaccaac catacatttg cactgcatca acattgaaat
    10681 aattactcaa ctcaataaaa tatgcatttg caatattggc aaacgagtac ataataatgc
    10741 ttccaaacaa tacagaaaca cgcccgcgct tatcgcccat gatgccccag atgatgccgc
    10801 ctacaagcat gcctatcatt tgcatattga gtaaatgttc gcctgttgat gtgagttgtg
    10861 cttgctcagt aataccaaca gcacgcaacg aatcgatacg ggtgatagaa aataaaatca
    10921 aatcgtatat atcaacaaaa tagccaagtg ctgctactat tacaattata tttaatggat
    10981 tggatgttgc ctgatgcttc ttttgcataa attatttttg agccgacaat atagttattt
    11041 tgttactctt ggttattggt aattgagtta ttggtaattg agttattggt tgttgggttg
    11101 ttgactttgc aatgttgagt gtcacagtga gtattctcga actgcgaagc atccctgcct
    11161 gaaaaggtgg tgcatcataa atatttttga agtaatttat ttagttcatt ttgcttttac
    11221 aatgtttatt gttgtccaag tattttactg tttcaagttc cgtaaatatt ctaaaattct
    11281 atacaacgga ttgcaagaaa caactttgta atttattttt cggaatcttc ccgaacgtta
    11341 aaagtttata aatgcgattg cgatataaat tgcttttgta aaaaaaataa aagcatttcg
    11401 aaatggattc tataaaccgg ggttgcttat tttttttatt ttagaatacg tatgcttcat
    11461 acgctttcgc atcctattgc ttgcaaagca gagatgcttc attcttgagc atttcaaaca
    11521 ctaaacaaat tcacagttaa aaaaactcag ttcattttct cccgcttaat caaatagggc
    11581 atcagcactt gttggtagcc tttgtgaatg tcggcttcta caatatcaat tttgtattgg
    11641 gcacatttaa tatttaattc ttttttaaac gcagccatgc tttcgaggta ttgttgtcgc
    11701 acttgattgc tgtgtacttt tacacgctcg ccactttcaa tatcgataaa ttcatacggg
    11761 cggttttcga aattgaaatc tatttctttc gatttatcta ctacgtgaaa taatactact
    11821 tcgtgtttat tatattttaa atgttgtagt gcaccaaaca gttcttcttg ctgttccatg
    11881 ttttcgaaca tatcgctaaa aataattacc agcgaacgct tatgtatact atctgcaatt
    11941 tgatgcagtg ctgctgctgc cgaagtttta ttgttgttgg ttgtgccttt tgcaattgca
    12001 gtatcaagat attgataaag taatttatga tgcaccgttg tggattttgc cggagtgttt
    12061 acttcaacat cattgctaaa taaactaagg ccaacagcat cgcgttgcat ttttaataaa
    12121 tacattattg ccgcagcact gtatgccgag aaagtaaatt tgttcaatag atttggattg
    12181 cgtgaatcta cttcggggta atgcatggat ccagaattgt cgacaattat atgacaacgt
    12241 aaatttgttt cctcttcgta acgctttaca aaaagtttgt cggttttgcc atataatttc
    12301 caatcgatgt ggcgtgtggg ttcaccggta ttgtacaaac gatgttcggc aaactctacc
    12361 gaaaaaccat gaaaaggcga tttgtgtaat cctgtaataa aaccttctac tacctgtcga
    12421 gcaagtaatt cgaggtgact tatgtgttgt atttcgtatt ggtttattgg cacaactttt
    12481 agaaattaaa aattaataat tgaaaattaa gaattaaaat gtagtaacaa gattttttga
    12541 ataaaggcaa acaccgaaat tgtttgacaa catctttaat caaaaaaaaa ctgttttagt
    12601 tttcatgctt caaataaagc aataagaaac taaaacagtt gttaatgaag tgtgaatgtg
    12661 cgcagtaaat tacgccaatt gggcatcgag tttttgcgcc aatgttgcct ttggtgctgc
    12721 accaatctgt ttgtctacaa tttttccgtc tttgaaaatt aataatgccg gaatatttct
    12781 aatgccaaac tgcatagaaa catttgggtt gtggtctaca ttaacttttc ccacaacagc
    12841 tttgccgtca tattctacag cgagctcttc tacaaagggg cctaccatgc ggcaaggtcc
    12901 acaccattct gcccaaaaat ctaccaatac cggaacgttt gattttagta ccaactcctc
    12961 gaagttggca tctgtaagtt ctaatgccat attattattt attaaaaaat tatatataaa
    13021 atctttatcc attgcccaat ggcaaatttc gtacaaagat aatcaatatg acaaaaaagc
    13081 atgacttatt tattcaacat cactttttgt aaatattgca gcgattgagc attgtctttg
    13141 ttaattgttt gttgttcgat gtatgttttt tcggtgctgg taagatgcca gctcaaaggc
    13201 acatgcaact cactttcggg tagctcgaat gttaccaaat ttatattctg gttatataaa
    13261 tcaagcacat actgtaagta agcatcgttg ttgtattcct gcattataat caggttgttg
    13321 gatacattgc gcagtggagc aaataaattt tgaatcaaag tctttgcgct attgtcgcga
    13381 aatgcagcat gtttgtatgt atcacgtatt tgtataatga ctacaccact tgtgttatcg
    13441 cttatccatt ttttaaaaac ctgtaaaaat ctacacgtgg tatgtaaacc aaaattatcg
    13501 cgtatgccgc tgtccataca ttccatagga ggaatacttg gcagcgatgc cggtggcata
    13561 atataaggga aggtggcatt catgcgtaag gcactgctaa attgtaaatc gagagcatcg
    13621 tggttggcaa acatctttgt aaattctata ccgttggtaa gtatttgtgt attgatgccg
    13681 ggtgcctttc ctgtttgcaa taaatatgaa accggctgtg gggtaatgta taaaacgcgt
    13741 ccatcgttta caattgtcga acttaacatt agcatgggaa tttgtgcatt ttgttcgggc
    13801 atgaggtaat ctttcaacct gtgttgcagt acattttcgg tatttttatt caactgccgt
    13861 tcccatgcat aggctctgtc ttttatatat ttgtgtttgc catcatcaaa cttttgaagc
    13921 gaaaaaaata aatcattaac tgttaagcta aaaatagtgg tgttcaataa atctttgccc
    13981 acatttattt tatcgttttt atttataaac ggatatggct gtttttgtaa cacagttcgg
    14041 ttataaaata attgacggta ataagccgcg ccaatcatac cgcccgatga gccggcaatc
    14101 atcattgtgt tgttcatcag ttgcccgttc aaaaccgaat cgatgcgttg taaagtttcg
    14161 aaagtaaaaa ccatgctgcg tgtaccacca ccacttacat taagcaatat taattttggt
    14221 ttcgatgcag tatcaattgt gtttttcttt ttccagtttt cgagaataga aatggtactt
    14281 ttgatgtcat catttacaaa tttagcatcg cacagtgagt cggtgagttg aagattataa
    14341 tttacagggg cagagtcata attcacgcca tacaattgat tgcgttgatt tacaaattcg
    14401 aaacccgaca cccagttaaa aaataaaagt atggcaatag ccatagtagt aacccacgag
    14461 ccaagccaaa acgaaaacaa tcccgataac ataatgaata aagtgaatag taaaaatata
    14521 cttgctgctg cgggaatttg aaaaacatct acatcgcgca aaaagccaag tgaaagaaaa
    14581 actaccaata ccgcagattg aaaaatggcc gcattgtaat ggtttttgcg aagaacaaat
    14641 tttaattttt cctgatcgta atgcgaagta tcgcgcacta attttatttt cgaaaaagat
    14701 gcaagataaa cctccacata cactatccat ttttttaaac gcatttgctc cacaattttt
    14761 ttgcgtgtaa acaaagttga tactgccacc atatcatcgt tgtcatcggt ggccgggcca
    14821 atgccgtaaa ttttgtaaat acttttgtca gttaaactaa aataccaaaa cgataacgaa
    14881 agaattaata tcatgccccc aagcaaggaa cactgtaaga ggataatatg tagcgcagaa
    14941 atggattgcg cattatattg aaattgcgcg attttaaaaa tgtagaaaaa aatgaatgca
    15001 acggggatga tacaattgtt tagagagtat ttgaaaaatg tacgcgacaa tgaagccaga
    15061 aatggaaagc gatgactgtt taataaatag ctggaaatgt tgtaggcaat aataaataca
    15121 cccagacata ttccggtaag gaagaacgaa aaataattta cccggccggc atattccggg
    15181 tcgaaaaaaa gatacggcac accattgcgt gccataagca gttcgccaac aaagcaaaaa
    15241 agtaaaagcc aatacaataa ctggaaatga tttttcttcg cttgcacaac aacaagctga
    15301 atgggaaaag aaaaaacaaa tcttcgtaca cgttgccgca caagcttatc cataaatcaa
    15361 aaaatgcttt gtattttttt tgttaaatgt atgtgagaaa agcaaaagca aaaatatgat
    15421 tacaataaaa tacgaacaaa gtagtacaca aaaatattga tgctatatgt tgttttcaag
    15481 tattgaaaaa attgatattt ttggctcaat aactatttaa tcaattcgaa atgtcacatc
    15541 agatttattg tttaaatatt tcctgattta taacattaaa ataaatatta catatggcat
    15601 acaatgaaga attggccgac agaattcgcg agaggcttgc ggaattgccc aacattgaag
    15661 aaaaaaaaat gatgggcgga ttaaccttta tggtaaatga taaaatgtgt gtcggtataa
    15721 taaaagatga attgatgtgc cgcatcgatc cggaatttca cgaaacggca gttgaaaaaa
    15781 acggttgccg aactatggat tttacaaagc gcccaatgat tggctatgta ttgatagatg
    15841 atacgggcat gcgaactcaa agagaatttg attattggat aaaccttgca cttgacttta
    15901 atgcaaaagc gaaatcttcg aagaagaaaa aatagtgttg ccaaactgtt tctttttaaa
    15961 tgatggtagc tatatttttc ttgaaacatt tttgtaaaga cagtgcaaca ttcaacagat
    16021 ggcttaaatt gaattccgca agaataaatc ccgtagagca tttctatttt ataacagact
    16081 gaccggcaat aattttactt ttagtgttgg gaatacttac aatggcttca cgcatttcat
    16141 aaggttggtc gaaaggcggt ttgctgtttg ccatatcgat tttgccttta acagtgttcc
    16201 aaaataattc tatatcatcc acttctaaat atatggaaac tgatgtgcat tcgctttttg
    16261 cctccgaaat atgaactgaa aaagcatcgt taaaaacaat agaataataa ccatagttgc
    16321 gaaagattga aaatcccaat acatcataaa agaaatttgt ggtgagggcc acatctgtcg
    16381 atggtattac aggacttagg tttatagctt tgtattttgt catttgtttt tgaataatta
    16441 aaaagctctt ttggatttat gcgcaaactg ttagcacatc caaaagagct cttttttatt
    16501 ttttttaaat aaatctattg agatatatta ttaagtcttt tccgaaaagt aaaataaatt
    16561 aaacagccac taaatcaccg aaacaccaaa ttcctcaaaa tattaaatat aatttgcttt
    16621 cgcttagtgg aatttcgtgc ctttgtgttt ttgtggcaaa aataattttt cgaagtggac
    16681 tcatttttat aatttttaaa acttattgtt ttacaacttg ctttacaaat gaagtattat
    16741 cgttactggc ttttatgata taaatacctt tattaatatt acccatatcg aaatttacaa
    16801 cctgtgaacc atttgttact ttactgaaaa tagtttttcc tgtaatgtcc attaactcca
    16861 cgttataaat tgctgcagtg ctaaaatgca attgcaaaaa attctgcaca ggattaggat
    16921 atacaattaa attatttgaa ttgctcgcag cagaagtaat gcccaacgac atagtaggat
    16981 ctataacaaa taatccattt tgcatatcgc tggcaacaat atttccactt gggaattcca
    17041 catacgaacc ccaactgcct gaataacttg gatttggcag accaagtccg tccgttggat
    17101 ttgtatcaat atatccggta cgcaccggtg tggcagggtt gctcacatcg aatatttgaa
    17161 caccatcctg atagtaagaa actacacagt aattatcttt catcataaac gggttgtgcg
    17221 gagtggctgt agacgcttgt gtggagcgga atgtagtcgg aattgtaatg tttgagaaat
    17281 cggtaatgtc gaaagttttt aatggcaatc ctgttggaac ttcatcggct accaccagag
    17341 ttttgctatc ggtggtgatt gctgtgctgt gattgtatcc cgaacccgga taaaccgtta
    17401 ctgaattata tattgtaaaa acatttgcac cggtatcata taaataaata tgaagaccat
    17461 cgaaacctgc cgaagcataa acggtatcgt tctttacaag catatcgtgt acgccaccta
    17521 ccgaaggatc atcctgattc aacttacgta ataaaacagg tgtggctgga tttataatat
    17581 cgtatacagc catagatgca gaattaccaa caacgccacc gcgtggactg gcagcataca
    17641 aatagtggtc gtcaataaaa atggtatgag ctgtttcaat tatattatcg ctttcatgca
    17701 ccacatgcac cgaatcgggg aggtaactca aatcggctac ttgtaggcaa ttcggtgggc
    17761 tatcgtcact taccatgaat aaaaaattct ggtaagtttt taattcacgc caaatgcaat
    17821 ccacacggca tccgggtaca aaatcgcaaa ctaccggtgt ggctggattg ctaacgttga
    17881 ttatatacgt acctgtagtt gctcctatga tagcatactc catcccattg ggtgatgtcc
    17941 agccccatat accattatac cttatgccat aataggattc ggccggcact gtgatgtcgt
    18001 cccaggttga aagcaatgtt acattttgcg aaggatattg cgcataagca atgcttgtaa
    18061 agagcattaa aattaatgtt agttttttca ttttagatta ttttattttt agatttaatt
    18121 tttaactgtt taataattgt gcaactatag cactcatttt tacttcggcc tcgttggcaa
    18181 cttttagtac ttcttcgtga ctcacacttt gtacatcatc aaaaccgcca aggtcggtta
    18241 caatgctcat ggcaaagcaa ttcatgccgc aatgttttgc tgctattact tcgggtacgg
    18301 tactcatgcc tacaacatcg gcacctataa tgcgcagata tttatattcg gcaggggttt
    18361 cgaaacatgg acccgacaca gctgcgtata cacctatgtg gcagcgatgt cccatttcat
    18421 cggcaataac ttttgcacgc gcaatcattt ctttgttgta aggcacactc atatcgggaa
    18481 agcgtgggcc aagattgtcg tcattttttc ctatcaatgg atttgatggc attaaattta
    18541 tgtggtcttc aatgatcata agatcgccca cctgaaaatt tttattcatg cctcccgcag
    18601 cattcgacaa aattaaatta tctatgccga gatatttcat tactctgatt ggaaaaacaa
    18661 cttgttccat tgtccatcct tcgtaaaagt gaaaacggcc ttgcattaca agcactggag
    18721 tattgccaag atggccaaac attagcttgc ctgcatgacc atgtacagtc gacacaggaa
    18781 aatttggaat atcactatag ctgatttcgc attcgctttt tatttttgtt accaggccac
    18841 ccaagcccga acctagcaca atgccgtact ttggtgtaaa agatgttttg ctttttatga
    18901 actctgctgt ttgtttaatt ttttctaaca taattttgca ttaacatatc gaacttcgat
    18961 ttttgattgt ttaaaaaacc tacttcaata ttttgtataa ataatttttc tttttcgaat
    19021 ggtaacttca ttgtacttaa ccttgcaaaa tcttttgctg tggttattag tattttattt
    19081 tcagtggcta aagtattaaa tttttcattg atagttacta agtcattata agtatactca
    19141 tgatgatcgg caaaggttaa caattcggtt ttggcaaatt gttcaccaat gtatttttgg
    19201 aggcttgaag gcttggcgat accagaaact aatagtgcca tatgtgtgcc tgctgctgcc
    19261 tgattaaaac tttggtttga aaatggattg tatagtgttg tgtaaattaa tgttgtaaaa
    19321 aataaatgtt ggtgtggatt tatttttatt tgttgtgcaa tatttttttg tgcttctatg
    19381 cttaaatcat ccggacattt tgttacgata ataatttgtg cccgtgatgc gcccgatatt
    19441 ggctcacgca aatttcctgc aggtaaaagc caatcgttgc aatataaatc gccatacgca
    19501 gtaagtacaa tatccatttc acttttcaaa tacctgtgtt gaaaagcatc gtcacaaata
    19561 accacatcat gcttttttat tttctgtaat tgttcaatgc catcaacacg tttttcgcac
    19621 accgcaaccg aaacagcatt attgtatagc ttaaaatatt cgtaaggttc atcgccaatt
    19681 gttgaagaat tgtcggagtt ggtagcaaat aaaaatcctg atgttttgcg tttatatccc
    19741 cggctgagaa tggcacataa tttttcttgc ttcagcaggt tggcaatata ttttaccatg
    19801 ggtgttttgc ctgtacctcc tgcttgtaaa ttgccaacta ttattacagg aaaaggaaat
    19861 tgtattgatt caataatttt aaaatcgaaa agtttattgc gcaaccaaac tacagcacca
    19921 taaattaatg caaaaggaaa aagtaaaatt cgcatggatt tgatttgcat gttattaaat
    19981 tactctgcaa gttgggcctg caatttctcg cgatttgttt gtgtgtcgta catggttttg
    20041 taaatgccgt tagcttctat caattgatgg tgcgaaccat gctccataat tttaccatca
    20101 tcaagaacat atatagtatc ggcagttttt accgatgaaa cacgatgcga aataatcacc
    20161 gttgttttat ccttcattat gcgttgcaaa ttttgcaaaa tggtttcctc tgtttcggta
    20221 tctacagccg aaaggcaatc gtcaaaaatt aaaatacgcg gttcctttac tatggcacgt
    20281 gcaattgata cccgttgctt ttgccctccc gaaagattaa taccacgctc gcccagcata
    20341 gtttcgaagc catcgttgaa gttgataatg ttatcataaa tggcggcatc atgtgcgctc
    20401 gctaacattt tttcgtgaag acttttttcg ttggtggtgt cttttatacc aaacaaaata
    20461 ttgtgcttta ttgtatccga aaataaaaat acttcctgtg gcacataacc aatttgttcg
    20521 cgaatatttt gtaaattatg ttgtgtaata tttttatcgt caattaatat ttcgccattg
    20581 gcagtatcat acattcgcaa taaaagattt gcgatggtgc ttttaccact gccggttttg
    20641 cctataaatg ctatgcactc accgggtcgt gcaacaaacg atatattacg caaggccgtt
    20701 attcccgaat tgctgtatgt aaaacttaca ttgttgaatg ctatctttcc atcaattgtt
    20761 ttatcatcgt tagttgatga ttttattgaa ataggaatgg ataaaaattc attgatgcgt
    20821 tgttgcgatg ctgctgcgcg ttgtatcaaa gtaataatcc aacccaacga tgccaccggc
    20881 caggtaagca tgtttacata aattacaaac tcggcaatat tgcctactgt tagttgatta
    20941 ttgattactg ctaaaccacc aacataaatt gtaataattg aactcacacc aataagtaaa
    21001 gtgatggaag gaaagaaaaa agcatttaca cgtgcaagtt ccatcgatgc atttttataa
    21061 tcggcacatt gtttatcaaa agcattttca aattcttttt cgcgggcaaa ggctttcaaa
    21121 actctgatgc ccgaaaagct ttcctgcaca aatgttgaca ttgttgaaag ttgcgattgt
    21181 actttgtcac tttttttatt gataatatcg tgcactttat atataataaa aaccagtatt
    21241 ggtaacggca acaaaacata aaaag
//
LOCUS       DAORQA010000022        20881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_649_length_20881_cov_19.601844, whole genome shotgun sequence.
ACCESSION   DAORQA010000022 DAORQA010000000
VERSION     DAORQA010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 20881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20881
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_649_length_20881_cov_19.601844"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PK736_03150"
     CDS             <1..198
                     /locus_tag="PK736_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32422.1"
                     /translation="LCISDKNYLNAINRIVDNKEFKGNVVKLSEEQKLMLQSSELDIE
                     NKRLIKHSNLMKEKKVWLQGK"
     gene            complement(821..4231)
                     /locus_tag="PK736_03155"
     CDS             complement(821..4231)
                     /locus_tag="PK736_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32423.1"
                     /translation="MRSKYISITTDTIAIDTLSIIPASLKVKTLDGKYVDDSLFIVDN
                     VTAKLVWNKTYLPLIKSLSDSMWISYRVLAVNLNKAYYNKTNNPQNLDKYGFSQYAYS
                     PRLDVAEDFFSTGGVSKIGSIARGISFGNNQDVVLNSSLNLQLAGKLNNNWEIAAAIT
                     DNNIPIQPEGNVQQLQDFDRVFIQLKSKDTKITAGDFDVVRPVSYFLSLQKRGQGLLV
                     NHNHILSKKDSTINSTTAGAAISRGKFARNVIPGVEGSQGPYRLTGAQNELFIIVLAG
                     TERIFIDGRLMVRGTQGDYTIDYNTAEVVFTARQLITRDVRIIAEFQYSDKNYLRTML
                     YVNNEFHRKKLNVRVNFFNEQDSKNQPLLQTLDDSAKAILTLAGNNPALAVIPQIDSV
                     AFNTNEILYAKIDTIVNTILYKNILRYSTNPDSAYYRVVFSQVGNSKGNYVQSITAAN
                     GRVYNWVAPVNGIPQGNYEPVTQLIAPKREQQLTAAADYQLGKYSNIVWEGSITNNDA
                     NLFSPINNKNNVAGASLLTYNIALPLDTHKTKPLKLFAKADWEFVQKKFTPIAFYRNP
                     EFIRDWNIINNSVAVDENIIAAAIGLHKDESRFLKYTLRSFLRDSLYSGYMNIVNGAY
                     MFHNFKINTVANYLTTRTQNESSRFLRSLSELSKQFKPIVTGVRFEQEENIMKAIRTD
                     SLYPVTNRFQWVTVFMNNAKENTAIDFNVQASKRWDDKVLSNILAPFSSADQAKIIAS
                     YKNKVHRIILNSQYRSLKYKLDSLTNNNETTVLNRIDYSVNINKGGINLNTSYETGNG
                     RELRKEYVFLETTPGQGVYTWIDYNKNGVKELNEFEVAQFTDQANYIKVLLPTNTYIK
                     AFTNSFTGILAINASQLIKSENKFSKWCKKFSNQFALKIDNKITSNKNGRGYNPFYSA
                     IADTSLIITNNSLRNNLSYNKSSSVWDADYLYTKTQAKNLLNSGYEFRTKEIHAINTR
                     VNFSSQFTLVVKGESISDNNKSEFFTSRNFSLASQQITSTFSYQYLSTFNASLIYLFG
                     NKTNASELKERAQQNNIGIECRLSGIKKGNLNTQFHFINLKYNGAENTPVAYEMLEGL
                     RTGINTTWNVNYQQNISKSLQITIGYEGRKSPTVKTVHVGNMTARAFF"
     gene            4637..5401
                     /locus_tag="PK736_03160"
     CDS             4637..5401
                     /locus_tag="PK736_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRC32424.1"
                     /translation="MKIITYNVNGLRSVLTKGWSEWLQSVNPDVVCLQETKASPEQLD
                     LTLFENMGYHHYWHSAQKKGYSGVSIFTKKKPTHIESGCGIKDYDNEGRVLRVDFGDV
                     SVMSVYMPSGSSGEERQAYKMQWIADFKKYIDELKKKRKKLIICGDYNICHRAIDIHN
                     PVSNAKSSGFLPEEREWMEQFINDGFIDTFRHFNKDPHHYTWWTYRFGAREKNLGWRI
                     DYTLVSQTLEKKLQRCVILPDAKHSDHCPVLLEIDI"
     gene            5440..5826
                     /gene="folB"
                     /locus_tag="PK736_03165"
     CDS             5440..5826
                     /gene="folB"
                     /locus_tag="PK736_03165"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRC32425.1"
                     /translation="MAEIVNRKKKGINMILEIKNIECFAHHGCLKEENSIGQKFSVDV
                     DLEIQNEKSVESDELGDTADYVLIHKLVRKEMSIKSNLIEHVAGRILQQIKENVAQAQ
                     TTTVKVTKYNPPVNGQIEKVAVIVKG"
     gene            5892..5962
                     /locus_tag="PK736_03170"
     tRNA            5892..5962
                     /locus_tag="PK736_03170"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5924..5926,aa:Cys,seq:gca)
     gene            complement(6022..9879)
                     /locus_tag="PK736_03175"
     CDS             complement(6022..9879)
                     /locus_tag="PK736_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32426.1"
                     /translation="MLSLISFSNRAKAIITKSFYNSTVLMYCMISVFYCNSLVAQNAP
                     VRQWSLSWPSGTNALSGSTGCIQAITQALDGGYVGVGYINVNGQSLQPSAIKVDAQGK
                     KLWEKVLDDNNTSIGILYDVIPVPNSTDVIGIGFKNGLVVFRLKQNGTIAAGFPKYFN
                     ATNMTGLGTFNPIGLSIRPYNVGGVHAGYVMCGDLFSCGTFGDQYQSAGGLVIKTDVN
                     FNLDLNFKSHANTAGWGKYNFGSNLFSFSDDRAAARCVRPINDASGNVTSFIITGQVY
                     DSPCVCGTPGGGCGTTAPPLNVFTARINLNGTVNWKTSLDETTIAGYNDIGPDTICTT
                     NGLPVENSSQIGFAAEQRTNGSNEIIILAGMDWTQSTLKSCSFVTGKLHNQTFCDLAL
                     FTINANAGGAVLANRKMNVGFCEGIEFRPSILIDRCTNSLFVSANHHGNNNVFTRIFK
                     IDISTNTFTRTWTKDAMGDEYSTISINNTFCMALAKDGGLVVAGCNESNTEGNFMLRL
                     ANDCQMNTVYDVDGLIINSGTVVWNTNKKVRGTIRIQNGGKLVINANAQIEFADTRQT
                     VDFELLHLNPGNLPTKIIVEPGARLVINGTAGTPKTTLTGLTSCYNCNSGANYNSEMW
                     EGIEVWGNPNLAQTFNATNTNQGYLNVYGNAVLQNMFKGISVDKTNYAAYPDTRINIG
                     YPANTGRNGGGVIQVTSASGAKFYNNIFSVVCSPFYGSNVNFTSLSNCSFNAAEFTNN
                     AVMRDLNYVDSLGKPLGVNTHMFVDNAKSITLSNCIFKGFATNALKQRGKGIYTNDAT
                     INCTSNNVITNFANLTYGIFANQSSAANQILNVSGNKFTNCYRSIVTRGTRNDNIRSN
                     TISIPNNTNSNVGINTWGSGNFTFDANNFTGATLPLSTTKSYGMLLHNTAAFASNAGG
                     NNFTNLNIGTQTQKNCGTAATNNGMQLFCNTYTNMLNSAWFIAPFNAGTLANQGQGCA
                     ATNKQARNIFIDPHNCISGAEVHIKSKAAFTYFNGNCVNCKPDCKSALVTTANCANDN
                     ASSCMLVNWPTTQAFADSIETLMGNTSNNQIKGTYRNTLVQYYLTKNDQTNAARILET
                     DSLSDNTKKILIAFYVGQRNWTKAQSKINQLTGAAGSDNDAYKIYFQLIKNVLAAGKS
                     LQQLSAAEIITVDSIATHESTASYNAQALLETYYSHKYPMYVETATGVEYKNGYVVNA
                     ANILIHVFPNPANDIATVQWEGNATTLMLTDVNGRIITALNIDDEEYQKAIATNDIDA
                     GIYFVTVFLSDGQQLVTKLVVSR"
     gene            10232..11389
                     /locus_tag="PK736_03180"
     CDS             10232..11389
                     /locus_tag="PK736_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HRC32427.1"
                     /translation="MLTSTVNITSKLPHVGTTIFTIMSGLANECKAINLSQGFPNFAV
                     SPALIELINKSMCEGMNQYSPMPGLITLREAIAKKTMLRFGTQYNPETEITITSGGTQ
                     ALFTAIMATVSKGDEVIIFDPAYDSYAPAVELAGGVPIHIALMPPNFNIDWNEVQQTI
                     SPQTRMIIINNPGNPSSQVLTKEDMIALQKITDGTNIIVLSDEVYEHIIFDGQQHQSV
                     SMFPQLAARSLVVYSFGKTFHATGWKMGYILGPQMLMTEFRKVHQFNVFSANTPIQAA
                     LSVFLSDENNYNYLEKFFAQKRDYFASMLLSSRFKILPCCGSYFQLLDYSAITSEHDK
                     DYAIRMTKENGVASIPVSAFYKNDTDNKLLRFCFAKDDATLQAAAERLCKI"
     gene            11402..12205
                     /locus_tag="PK736_03185"
     CDS             11402..12205
                     /locus_tag="PK736_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRC32428.1"
                     /translation="MATKNNLKITLVQTKLHWENIDANIEMFDEQLKGIKKSSTHLIV
                     LPEMFTTGFTMNAAENFSEMNGAAIMWMKKMAAKKNCAVCGSLIIKEDGKYFNRFVWI
                     DAEGKIQCYDKRHLFRMANEHLTYTAGNDHLVVELNGWKIMPQVCYDLRFPAWSRNTN
                     DNGKMKYDLLLYVANWPERRKQAWKQLLIARAIENQCYCIGLNRVGKDGKGINYTGDS
                     AVIDFLGNNISKIAPSRSSITQTTIELSPLQKYRNDFPAYMDADVFELK"
     gene            12621..13079
                     /locus_tag="PK736_03190"
     CDS             12621..13079
                     /locus_tag="PK736_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRC32429.1"
                     /translation="MQSKATTVEKYLDEIPTDRKAAFAKLRNVILKNIPKGYKEQMTY
                     GMIGYVIPHSIYPKGYHVDPKLPLGLAAIASQKNFVAFYHMGIYAHPELLKWFVSEFP
                     KHSKWKLDMGKSCVRFKKPDDIPFDLIGELMTKITTKMYIELYEKNVPSK"
     gene            13257..14480
                     /locus_tag="PK736_03195"
     CDS             13257..14480
                     /locus_tag="PK736_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32430.1"
                     /translation="MKTNYLQKIAIIVSLSISCYNINAQITNYVYALHNTQSGIFYFS
                     EIEIATGNITDLQLMPVTGYGTYSCSAVNVTTQEFYFCDGTQLISIDANNGNINSNIT
                     LPIPPLTNFSQIQYNQCDSNIYGLIKDNTNMVGFAKFNPYTGVFTNISSLNSSIGYCF
                     GCMTVIDPVANFFAFQSNGVIVIEITTGNIIYNSPLNNLPNENFGHIALKCSTHEIFG
                     TSANVSTGLKYLATIDPATGNVSHVSANGWTPGLFKPGAGGDFINQFTGDYFYSGANS
                     KLLGANTITGNLVYNKTIGNQGEILFINHFSSCGCGSPSAINNVAPQNNTIEILPNPF
                     SNYINVKNIVTNQKVYFQLISPLGKIVFASIINSSEPINVSQLNLPQGIYHYRIFDEQ
                     QSKVLQTGNIMHTQD"
     gene            complement(14678..16309)
                     /locus_tag="PK736_03200"
     CDS             complement(14678..16309)
                     /locus_tag="PK736_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32431.1"
                     /translation="MKNPYVVTFVDLSLQDGMAYDNDKKSFTSYCQNCNCPMKLIFKL
                     TTMIVIMVVFCYIAKAQAPNSWIMFGFPNNNQLNFFPNVSTSNVNGVYHQLFYSNASI
                     GDSSGNLLYYTNGYTIWNSQHTKIMNDDTLCPSYYTSVTYNSGPNLWQMDVFIPDPGD
                     TNEFYLFYAGGDIIYFYPNGGGDYLPNKFYYAKVNKTINGLNGFASTINNVILNDTIG
                     SGVCAIKHGNGRDWWIVVRHVYKPEFIRFLVDPSGVHGPYYQNIGPVNYAGFVNFKFA
                     PGTNKIACSNAWHLVNEQWFDIYDFDRCTGLASNPITLHFYDNSFNWFHVNGGCEFSP
                     SGQYLYVSTTMKLRQFDMLAPNIAASGIVIDSLSVIAPIESNSRMQLGPDNKLYIDIN
                     NGGSQWLHVIHQPDSPGFACNYDDTAITFIYPISSTMPNIPHYEIGAMVGSPCDTLSA
                     NSPPAPQRGEQQLYAIYNTLTQQLVVTANNIKGKNVTVSIYDGRGSLKFEVISLKSNA
                     GYFTLDVDCAGWSDGLYVVHLQTEKEVLSKKFVKE"
     gene            16484..18565
                     /locus_tag="PK736_03205"
     CDS             16484..18565
                     /locus_tag="PK736_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRC32432.1"
                     /translation="MLTQSKKKTSIGEDTLLSAFEKMCTAKAMTEIYEANAQFTSKYV
                     HATSRGHEAVQIALGLQLQPQDYLSAYYRDDSILLSIGLQPYELMLQLMAKRDDPFSG
                     GRTYYCHPSLRREGMPTIPHQSSATGMQAIPTTGIAMGIQYKELQGINEWTALDAPIA
                     VCSLGDASVTEGEVSEAFQMAVLKQLPILYFVQDNGWDISATAKETRAMDANEYAQGF
                     KGMEVSSIDGTDFTTCFETIQDVISIMRAERRPFMIHAKVPLLNHHTSGVRKEWYRDD
                     LEEAAQRDPFPKLRKLLLQSGIDESKLILIENDARALVEADFIKSKNAEDPRPEDLFL
                     HEFAPTTITEERGIRQPEGKEKTVMVDSALFAIQELMAKHAECLLYGQDVGRRLGGVF
                     REAATLAQKFGDHRVFNTPIQEAFIIGSTVGMSAAGLKPIVEVQFADYIWPGLNQLFT
                     EVSRSCYLTNGKFPVSCIVRVPIGAYGSGGPYHSSSVESILTNIKGIKVVYPSTGADL
                     KGLMKAAYYDPNPVVMLEHKGLYWSKIKGTEDAKTIEPDEEYIIPLGKARTVIIADDD
                     KIKSSESMVIITYGMGVYWSKNAAKNFEGKIEIIDLRTLYPLDEVAIFDAVKKHHRAL
                     IVTEEPYNNSFAQALCGRIQQHCFQYLDAPVMVIGSENLPAIPLNTTLEFTMIPNTEK
                     VGVMIEKLLAY"
     gene            complement(18784..20304)
                     /locus_tag="PK736_03210"
     CDS             complement(18784..20304)
                     /locus_tag="PK736_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32433.1"
                     /translation="MKIIFTLLIFLFFTFNGQSQFKNNIWCFGDSAGINFVNPNSPST
                     FITSVKSRGSCVSICDTNSNVLFYAYTRAGLAGNTTLVKDRNNNPMQNGTNVLGQGWY
                     YELIIVPFPSDTNKYYLFSIGVSNSSPYGLYYSIIDMTLNNGLGAVTQKNIQVNNMKA
                     NDGLTAIQHGNGRDWWLLFRGVTTNLNNTYFKYLIDKNGVLLNDTQNIGSITNIGFIK
                     YSFDKTGNKLVMVYYNGLCETFDFDRCTGQLSNNVSVRPPTPVPPPIPEFWSCDFSPN
                     GRFLYIATSGYYNAAYLIQVDLLNPLLYATADTIDSITGLESPGGQLKRGPDDKIYWS
                     CAWIDSLGTWNYPYPNTPASFNMYNMNLSVINSPDSLGAACNFTPYSFYLGGARTYWG
                     LPNNPDYDLGPLKGSPCDTLTLLTPALSKGEGVMQITYVAAWEKLFVNASGLKGKNVT
                     ITIYDGRGSLMFEVQSLKAVNGYFTLDLDCSGWAGGVYVVHLETEREVLSKKFIIE"
BASE COUNT         6658 a         3985 c         3836 g         6402 t
ORIGIN      
        1 ctgtgtataa gcgacaaaaa ttacttaaat gcaattaatc gcatcgtaga caataaggaa
       61 tttaaaggca atgttgtcaa acttagtgaa gagcaaaagc taatgttaca atcaagcgaa
      121 ttggatattg aaaataaaag gttaataaag catagcaatc taatgaaaga aaaaaaagta
      181 tggttgcaag ggaagtaatt tggacaacca cagccgaaag acaacttcaa aacacacttg
      241 agtattggtt gaataggaac aaatcaccgt tatattcaca aaaacttttg caattagttg
      301 ataaatacat ccaacaaatt tgtcagcgac caacatcttt caaactaacc gaatatgcca
      361 accatcgtgt ttgtgtgatg ggtgtttata gtctttactt tttgtacagc aacaatcaaa
      421 ttatcgttac gggtttttgg gacggtcgtc aaaatccaaa aggattgaga aaaaaattaa
      481 gcaaaagaaa gtaaaatttt aaagctgttt aaagagattg atagcacctt gcacaccgcc
      541 ataaacgatg ctttgataaa tcaaatgccg aatagttttc ttaaccaatt agcaggagtg
      601 cagaaatcac aaatttcaaa attagaaagc agtgcaaata gtgctacaat tattaaaatt
      661 tttcaggcac ttaaggcaga aataaatttc aatattaagt tggaaaaaca gtacatgaaa
      721 ttagtatagc caaagcaacg gaaagcctca atgcacaaag gaaaattaat gacttttcta
      781 caggattcag agtaagaaat ttttttaaaa aaccattgtc ctaaaaaaac gccctcgcag
      841 tcatgttgcc tacatgtaca gtttttactg ttggcgattt gcggccttcg tagccgattg
      901 ttatttgcag cgatttcgaa atattttgtt ggtagtttac attccatgtt gtgttgatgc
      961 cggtgcgtaa accttcgagc atttcgtaag ctacgggtgt gttttcggca ccgttgtatt
     1021 ttaaatttat aaaatgaaat tgtgtattga ggtttccttt ttttattcct gataagcggc
     1081 actcaatgcc aatattattt tgttgtgctc gctccttcaa ttccgaagca ttggttttat
     1141 ttccaaataa ataaataagc gaagcattga aagtgctaag atattgatac gaaaaagtag
     1201 aagtgatttg ttgcgatgct aaactaaaat tgcgcgaagt aaaaaactcc gatttgttgt
     1261 tatcacttat cgattcacct tttaccacaa gcgtaaattg cgaagaaaaa tttacacgcg
     1321 tattgatggc atgtatttcc ttggtgcgaa attcgtaacc gctgttcaat aaatttttcg
     1381 cctgtgtttt agtatacaaa taatcggcat cccaaaccga agacgattta ttgtaactca
     1441 aattattgcg cagcgagtta ttggtaatga taagtgaggt atcggcaatg gcagaataaa
     1501 atggattgta accccgccca tttttatttg atgttatttt attatcgatt tttaaagcaa
     1561 actgatttga aaattttttg caccacttgg aaaatttatt ttcactcttt atcagttgcg
     1621 aagcatttat ggcaagtata cccgtaaacg aatttgtaaa tgctttgata taagtatttg
     1681 ttggcagcaa cacttttata taatttgcct gatcggtaaa ttgtgctact tcaaattcgt
     1741 tcaattcctt tacaccattt ttattataat cgatccacgt atacacacct tgtccgggag
     1801 tagtttccag aaacacatat tcctttctca actcgcgacc atttccggtt tcgtacgatg
     1861 tgtttagatt tatgccaccc ttgtttatgt ttaccgaata atcaatacga ttgagtactg
     1921 ttgtttcatt attattggta agcgaatcga gtttatactt taagcttcgg tattggctgt
     1981 tgagaataat gcgatgcact ttgtttttgt aagaggcaat tatttttgcc tgatcggcac
     2041 tgctaaatgg tgctaagata ttcgatagaa ctttatcgtc ccaccgtttg gaggcttgta
     2101 cattaaaatc gatggcggta ttttctttgg cattattcat aaacactgta acccattgaa
     2161 agcgattagt aacaggatac aacgaatcgg tgcgtatggc tttcataata ttttcttcct
     2221 gttcaaatcg cacacctgta acaataggtt taaattgttt cgacaattcg ctaaggcttc
     2281 gcaagaaacg tgacgactca ttttgtgtgc gtgttgtaag ataatttgcc accgtattaa
     2341 ttttaaaatt atgaaacatg tatgcgccat tcacaatatt catataacca ctgtaaagag
     2401 aatcgcgcaa aaaactgcgc aaagtatatt tcagaaaacg gctttcatct ttatgcaaac
     2461 caatggcagc agcaataata ttttcgtcaa cagcaacact gttatttata atattccaat
     2521 cgcgaataaa ctcggggttg cgataaaaag caatgggagt aaatttcttt tgtacaaatt
     2581 cccaatctgc ttttgcaaat agttttagcg gttttgtttt gtgcgtatcc aatggcaaag
     2641 caatattata tgtgagcaaa ctagcaccgg ccacattgtt tttattattg atgggcgaaa
     2701 ataaattcgc atcgttgttg gtgatgctac cttcccaaac aatgtttgaa tatttaccca
     2761 attgataatc ggctgcagcg gttaattgtt gctcgcgttt gggtgctatc aattgtgtaa
     2821 cgggctcgta attgccttgt ggtattccat ttacgggtgc cacccaatta tacactctgc
     2881 catttgcagc agtaatgctt tgcacataat tgcctttgct attgcccact tgcgaaaaca
     2941 ctacacggta atatgcacta tcgggattgg tagaatagcg caaaatattt ttgtataaaa
     3001 tggtatttac aatagtatcg attttggcat acaatatttc gttggtatta aaagcaacgc
     3061 tatctatttg tggtataacg gcaagcgcag gattgtttcc ggccagagta agaatggcct
     3121 ttgccgagtc gtcaagtgtt tgcagcaacg gttgattttt actgtcctgt tcattaaaaa
     3181 aattaacacg cacatttaat tttttacgat gaaattcgtt gttcacatat aacatggtgc
     3241 gcaaataatt tttatcgctg tattgaaact cggcaattat gcgcacatcg cgagtaatga
     3301 gttggcgggc ggtgaataca acttcggcag tgttatagtc aatagtgtaa tcgccttgtg
     3361 tgccacgcac catcaatcgt ccatcaataa aaatacgctc ggtgccggca agcacaatta
     3421 taaataattc gttttgggct ccggtaagcc ggtacggtcc ctggcttcct tctacaccgg
     3481 gaataacatt gcgcgcaaac ttaccacgcg atatggcagc acctgcagta gtagaattta
     3541 tggtgctgtc ctttttactt agtatatggt tatggttaac aagcaaccct tgtccacgtt
     3601 tttgcaaact aagaaaataa cttacaggcc ttacaacgtc aaaatcgcct gcagtaattt
     3661 tggtgtcttt gctttttagc tgaataaata cacggtcgaa atcttgcagc tgttgtacat
     3721 ttccttcggg ctgtatagga atattattgt cggtaatggc tgcggcaatt tcccagttat
     3781 tatttaactt accggctaat tgcaaattta aagatgagtt taataccaca tcctgattat
     3841 tcccaaacga aataccgcgg gcaatacttc caattttgct tacaccaccg gtagaaaaaa
     3901 aatcttcggc cacatccaga cgtggagaat aagcatattg cgaaaaacca tatttatcca
     3961 gattttgcgg gttgttggtt ttattatagt acgccttatt taaattcacg gccagcacac
     4021 ggtagctaat ccacatgcta tcgcttaaac tttttatgag tggcaaatac gttttattcc
     4081 aaaccaattt agcggtaaca ttatctacaa taaagaggct atcgtcaaca tattttccat
     4141 cgagtgtttt tacttttaac gatgcaggaa taatactcag cgtatctata gcgatagtat
     4201 ctgttgtaat ggaaatatat ttactccgca cattgcttag tggttgggca tttagggtgc
     4261 tacaatttaa acatgctaaa atgaagcacg aaaaaagtat aactctataa acaaaatagc
     4321 ctggcactat aacttaattt ccatttttgt aaaacgcatt ggcatcacat aaattttcga
     4381 aaggctaaat taactaatgt ttgcccaact atttatgccg aaacaactta ggttttggaa
     4441 aaacaagctt ttgaagttgt tcggcattac taagagtacg ttatattttc attcgaaaat
     4501 ctaactactc ttagtataaa tagcaacagg tgtggtaacc aacatataag tgtaagtaag
     4561 tattatcata ttattttctc aaaaagatat atcattgcca acctcaactt ttggtaaaaa
     4621 aagataaagg ataatgatga aaataataac atacaatgta aacggtttgc gcagtgtact
     4681 taccaaaggc tggagcgaat ggttgcaaag tgtgaacccc gatgtggttt gcttgcagga
     4741 aacaaaggca tcgcccgagc aactcgattt gacacttttt gaaaacatgg gttaccatca
     4801 ttactggcat tcggcacaaa agaaaggata cagtggcgta tcaattttca caaagaaaaa
     4861 acccacacat attgaatccg gatgcggcat aaaagattat gataatgaag gcagggtgct
     4921 tcgagttgac tttggcgatg taagtgtgat gagtgtatac atgccatcgg gcagtagtgg
     4981 cgaagagcgg caagcatata aaatgcaatg gatagccgat tttaaaaaat acatagatga
     5041 gttgaaaaag aaacgtaaga aattaatcat ctgtggcgat tataatattt gtcaccgtgc
     5101 aatcgatatt cacaatccgg tgagcaatgc aaagtcatcg ggatttttgc ccgaagagcg
     5161 cgagtggatg gaacaattta ttaacgatgg ttttattgat acgttccggc attttaataa
     5221 agacccacat cattatactt ggtggacata tcgttttggt gcacgcgaaa aaaatcttgg
     5281 gtggcgtatc gattatacat tagtatcgca aacactagaa aaaaaactgc agcgatgtgt
     5341 tattcttccc gatgcaaaac atagcgacca ctgtccggta cttttggaaa tagatattta
     5401 gcaaaaagtg aaaaccaaag tccacggtat aaaatggatt tggcagaaat agttaatcgt
     5461 aaaaaaaaag gaataaatat gatactcgaa attaagaata tagaatgctt tgcacatcat
     5521 ggatgtttga aagaagaaaa cagcatagga caaaaatttt cggttgatgt tgatttggaa
     5581 atacaaaatg aaaaaagtgt ggagagcgat gaactaggcg acacagccga ttatgttttg
     5641 atacataaac tggtgcgtaa ggaaatgagt ataaaaagca atcttattga acatgttgcc
     5701 gggcgtatat tgcaacagat aaaagagaat gttgcacagg cacaaaccac tacagtaaaa
     5761 gtaacaaagt ataacccacc tgtaaatggc caaatagaaa aagtggcggt gattgtaaag
     5821 ggctaaactt ttacccaata ggatttgcaa accaatatat ttttctattt ttgcgcctcg
     5881 aataataaaa gggtcctgtg gccgagtggt taggctcagc tctgcaaaag ctgctacagc
     5941 ggttcaaatc cgctcgggac ctcaaaaaaa atcctctgtg gctcacgctt cagaggattt
     6001 tttatttttt gcaattcgca cttatcggct caccacaagt tttgttacca attgttgacc
     6061 atcggataaa aacactgtca caaaatatat tcccgcatct atatcgtttg ttgctattgc
     6121 tttttggtac tcttcatcgt caatgttcaa tgctgtaatt atgcgcccat ttacatccgt
     6181 taacatcaat gtagtggcat tgccttccca ttgcactgtt gcaatatcat ttgccggatt
     6241 tggaaaaacg tgaattaaaa tattggcagc attcactaca taaccatttt tatattcaac
     6301 accagtggcc gtttctacat acataggata tttgtgacta taatatgttt cgagcaaagc
     6361 ttgcgcatta tagcttgctg tgctttcgtg cgtagcaata gaatcaacag taataatttc
     6421 tgctgccgat aattgctgta aactcttgcc tgctgccaat acatttttta tcaattgaaa
     6481 ataaattttg taagcatcgt tatcgctgcc ggctgcacct gtaagttgat ttattttgct
     6541 ttgcgctttt gtccagttac gctgccctac ataaaacgcg atgagtattt tttttgtgtt
     6601 atcgctaagg ctatcagttt cgagtatacg tgcggcattg gtttgatcgt tttttgtaag
     6661 ataatattgc accagtgtat ttcggtatgt gccttttatt tgattattgg aagtgttgcc
     6721 catgagtgtt tcaatagagt cggcaaacgc ctgggtagtt ggccagttaa caagcataca
     6781 gctcgaagca ttatcgttgg cacaatttgc agttgtaaca agcgcacttt tacaatcggg
     6841 tttacagttt acacaattac cattgaagta agtaaaagcg gctttcgatt ttatatgaac
     6901 ttcggcaccg ctgatacaat tgtgcggatc gataaaaata ttgcgcgcct gtttgtttgt
     6961 tgctgcacaa ccttgcccct gattggccaa tgtaccggca ttgaatggag caataaacca
     7021 ggcactgtta agcatattag tataagtgtt acaaaacagt tgcatgccgt tattggtagc
     7081 tgcggtacca caattttttt gtgtttgtgt accaatgttc agatttgtaa aattgttgcc
     7141 tcctgcatta cttgcaaacg cagcagtatt gtgtaacaac atgccataac tttttgtggt
     7201 tgataaaggc aaagtagctc ctgtaaaatt attggcatcg aaagtaaaat ttcccgagcc
     7261 ccatgtattt attcccacat tcgaattggt attgtttgga atgctgatgg tgttacttcg
     7321 tatattgtcg ttgcgggtgc ctcgcgtaac aatactgcga tagcaattgg taaatttatt
     7381 tccactcaca ttcaatattt gattggctgc cgaactttga ttagcaaata tgccgtaagt
     7441 aagattggca aaatttgtaa tcacattatt cgatgtgcaa ttaatggttg catcgtttgt
     7501 gtaaattcct ttgccacgtt gcttcaatgc attggttgca aatcctttga atatacaatt
     7561 tgaaagcgtt atcgattttg cattatcaac aaacatatga gtgttcacac caagtggttt
     7621 accaagactg tcaacataat ttaaatcgcg catgacagca ttgtttgtaa attctgccgc
     7681 attaaatgag caattgctta gcgaagtaaa attcacattg ctgccataaa aaggcgaaca
     7741 tacaacactg aaaatgttgt tataaaattt tgcaccactg gcagaagtaa cttgtattac
     7801 accaccaccg ttccttcccg tatttgccgg atagccgatg tttatgcgcg tatcgggata
     7861 ggcagcataa tttgttttat ctaccgaaat acctttaaac atattttgta aaacagcatt
     7921 gccatataca tttaagtaac cttgattggt attggtggca ttaaaagttt gtgctaaatt
     7981 gggattgccc catacttcga tgccttccca catttcgcta ttataatttg ctccgctatt
     8041 acaattgtaa cacgatgtta aacctgttaa tgttgttttg ggtgtgcccg cagttccatt
     8101 tattaccaat cgggcaccgg gttcaactat tatttttgta ggtaaatttc ccgggttgag
     8161 gtgcaataat tcgaagtcga ccgtttggcg tgtgtcggca aattctattt gtgcattagc
     8221 gtttattaca agcttgccac cattttgaat gcggatagta ccacgcactt ttttatttgt
     8281 attccaaacc acagtgcccg aatttatgat taaaccatca acatcataca ctgtattcat
     8341 ttgacaatcg ttggctaagc gcaacataaa atttccttcg gtattacttt cgttgcaacc
     8401 tgccacaacc aatccaccat cttttgccag tgccatacaa aaagtgttgt ttatacttat
     8461 agtgctgtac tcatcgccca ttgcatcttt tgtccatgtg cgggtaaatg tatttgtgct
     8521 gatatcaatt ttaaaaatac gcgtaaacac attattgttt ccatgatgat ttgcacttac
     8581 aaacaaacta tttgtacacc tgtcaattaa tatacttgga cgaaactcta tgccttcgca
     8641 aaaaccaaca ttcatttttc ggtttgccaa tactgcacca ccggcatttg catttattgt
     8701 aaataatgcg aggtcgcaaa aagtttggtt atgtaatttt ccggttacaa agctgcacga
     8761 ctttaatgtt gattgcgtcc agtccattcc ggcaagaata attatttcgt tgctgccatt
     8821 ggtgcgctgt tcggctgcaa accctatctg gctgctgttt tcaaccggta gtccgtttgt
     8881 agtacaaatg gtatcggggc caatatcgtt atagccggca atggtagttt cgtcaaggct
     8941 tgttttccaa tttacggtgc cattcaaatt gatgcgtgcc gtaaaaacat taagtggcgg
     9001 tgctgtagtg ccacaacctc cacccggtgt gccacacaca catgggctat cgtatacttg
     9061 ccccgttata ataaacgatg taacgtttcc cgaagcatca tttataggac gcacacaacg
     9121 tgccgctgca cgatcatcac tgaaagaaaa taaattactg ccaaaattat atttacccca
     9181 accagcagta tttgcatggc ttttaaaatt caaatcgaga ttaaaattca catcggtttt
     9241 tattaccaat ccacctgccg attgatattg atcgccaaaa gtgccacatg aaaataaatc
     9301 gccacacata acataacctg catgcacacc gccaacattg taagggcgga tagataaacc
     9361 tatgggattg aaagtgccaa gtcccgtcat atttgttgca ttaaaatatt ttggaaaacc
     9421 tgctgcaatg gtcccatttt gtttaagcct aaaaactacc aacccatttt taaatcctat
     9481 gccaataaca tctgtcgaat taggaacagg aataacatcg tacaatattc ctatagaggt
     9541 gttgttatcg tcaagaacct tttcccacaa tttttttccc tgtgcatcaa cttttattgc
     9601 cgaaggctgc aacgactgtc cgtttacatt tatataacca acaccaacat aaccaccgtc
     9661 caatgcctga gtgatggctt gtatgcaacc ggtagatcct gatagtgcat tggtgccact
     9721 tggccacgat aaactccatt gccgcaccgg agcattttgt gcaacaaggc tgttgcaata
     9781 aaaaacggat atcatacaat acatcaacac agttgagttg taaaacgatt ttgttataat
     9841 cgcttttgcg cgattggaaa aagaaataag tgacaacatt gttttttgtt tttatcttaa
     9901 aatgtaaagt aacattatga atttcgaaat tgaaataaga agcaaaaaag tggcttccga
     9961 atacaaatta tttatatcaa aaatctatcc attaaaacac agtgggcgaa aatctcaaca
    10021 ccaaagtatt tatgtaataa ctttataccg aaacaactca ggttttggaa aacaagctat
    10081 tgaagttgtt cggcattact aagagtacgt tatattttca ttcgaaaatc taactactct
    10141 tagtataaca gaaatgcatt tatattttct acgaaattag aataacaatg cgataaaaca
    10201 catatttgta aaacgatttt aatttttgaa tatgctaaca tcaactgtaa acattacttc
    10261 gaaactgcca catgtgggca ctaccatttt tacaataatg agtggccttg ccaacgaatg
    10321 caaggctatc aatctctcgc aagggtttcc caactttgct gtatcaccgg cattaattga
    10381 gcttataaat aaaagtatgt gcgagggaat gaatcaatat tcgccaatgc cgggattgat
    10441 aacattgcgc gaagcgatag caaaaaaaac catgttgcgt tttgggacac aatacaatcc
    10501 cgaaacagaa ataaccatca cttcgggagg tacgcaagca cttttcactg ctataatggc
    10561 cactgtgagc aagggcgatg aggtgataat ttttgacccg gcttacgact cgtatgcacc
    10621 ggctgtagaa cttgccggag gcgtgccaat acacatagca ttgatgccac ccaattttaa
    10681 tatcgactgg aacgaagtac agcaaactat ttcgccacaa acgcgaatga taattattaa
    10741 taatccgggc aacccatcat cgcaagtgct taccaaagag gatatgattg cgttgcaaaa
    10801 aataaccgat ggcacaaaca taattgtgct tagcgatgaa gtgtacgagc atattatttt
    10861 cgatggacag caacaccaaa gtgtgagtat gtttccgcaa ctcgcagcgc gaagtttggt
    10921 ggtatattct ttcggtaaaa cttttcatgc tacgggttgg aaaatgggtt acatccttgg
    10981 gccgcaaatg ctgatgaccg agtttcgaaa agtgcatcag tttaatgtgt tttcggctaa
    11041 cacgccaata caggcagcgc tatctgtttt tcttagcgat gaaaataatt acaattatct
    11101 cgagaaattc tttgcacaaa agcgtgatta ctttgcttca atgctattat catcgcgttt
    11161 caaaatattg ccttgctgtg gttcgtattt tcaattgctc gattactcgg caattacaag
    11221 cgagcacgat aaagactatg ccatacgtat gacaaaagaa aatggtgtgg cttcgatccc
    11281 tgtttcggca ttttataaaa atgataccga taataaatta ctgcgttttt gttttgcaaa
    11341 agatgatgca actttgcaag ccgctgccga acgtttatgt aaaatataat ttcacaaaaa
    11401 aatggctaca aaaaataatt tgaaaataac actggtgcaa accaaactgc actgggaaaa
    11461 tattgatgcc aatatcgaaa tgtttgacga acaattgaag ggcataaaaa aaagcagtac
    11521 acaccttatt gtattgcccg aaatgtttac aacaggtttt acaatgaatg cagcagaaaa
    11581 cttttcggaa atgaatggtg ctgcaataat gtggatgaaa aaaatggccg cgaaaaaaaa
    11641 ttgtgctgtg tgtggcagct tgattattaa agaagatgga aagtatttta atcgttttgt
    11701 gtggattgat gctgaaggca aaatacaatg ttacgataag cggcatttgt ttcgcatggc
    11761 aaatgaacat ttgacttata cggcaggcaa tgatcatctt gtggttgaat taaatggatg
    11821 gaaaataatg ccacaagtgt gttacgattt gcgattccca gcatggagcc gcaatacaaa
    11881 cgacaatgga aaaatgaaat atgatttgtt gttatatgtt gccaactggc ccgagaggcg
    11941 caaacaagca tggaagcaat tattgatagc acgtgccatc gaaaatcaat gttactgcat
    12001 tggattaaat cgcgtgggca aagatggaaa aggaataaac tatacaggcg attcggcagt
    12061 gatagatttt ttgggaaata atatttctaa aattgcccct tcgcgaagta gcataacaca
    12121 aaccacaata gagttatctc cattgcaaaa ataccgcaac gattttcctg cttatatgga
    12181 tgccgatgtg tttgagttga aatagtattt ccaattattt ctgagcgctc tttgcggtaa
    12241 aaaaaagtaa ccgcaaaggc cgcaaagaaa caccgcaaaa ttcgcaaaga agaaaagact
    12301 acacacacgg aattatttaa taacaaaaat gaaggtctgt atttcttata tatttctttg
    12361 cggaattctt ttgcgctctt tgcggtaaaa aaaataaccg caaaaaactt cactaagttg
    12421 taaacgaatt aattttaatg ttttgtggga tttagcgttt tggtgattta gtggctatta
    12481 gtatttgtaa gagtagaaac attttaatag attttttact ttttttgtaa gggtaatagt
    12541 gctgtaaaat attttcagtt caatgtattt tgttttttat ttttgtgagg cataaaatat
    12601 tttaaaaaca aacttttacc atgcaatcga aagctactac agttgaaaaa tatttggacg
    12661 aaattcctac cgatcgcaaa gccgcatttg caaaacttcg caatgttatt ttaaaaaata
    12721 ttcctaaagg atataaagaa caaatgactt atggaatgat tggctatgtg attccacatt
    12781 ccatttatcc aaagggatat catgttgatc ctaagttgcc gttgggactg gcggctattg
    12841 catcacaaaa aaatttcgtg gcattttatc acatgggcat atacgctcat cccgaattgt
    12901 tgaaatggtt tgtgtccgaa tttccaaagc acagcaaatg gaaattggat atgggaaaaa
    12961 gttgtgtgcg atttaaaaag cctgacgata ttccttttga tcttataggc gaactgatga
    13021 ccaaaatcac caccaaaatg tatatcgaac tttacgaaaa aaatgtaccg agtaagtagg
    13081 ttttaaaaaa acgataagca tcggtgttca tacaatgtat tttaaatttg tcatacgcca
    13141 tttttgtttt tctttttttt atttctcatg cttataaatt ccacaaggaa atattttttg
    13201 gtttgcgtta tttttattct gtaaatttga acatcattat ataaaacgta tgctctatga
    13261 aaacaaatta tctacaaaag atcgccatca ttgtttcgct tagcatttca tgttataaca
    13321 tcaacgcgca gattacaaat tatgtttatg cattgcataa tacgcaatca ggcatttttt
    13381 atttctccga aattgaaatc gccaccggca acattaccga tttgcaactt atgcctgtta
    13441 caggatatgg tacttacagt tgttcggctg taaatgttac cactcaagaa ttttactttt
    13501 gtgatggaac acaactcatc agcattgatg ccaataatgg aaatataaat tcgaatatta
    13561 cgcttcccat tccaccgcta acaaactttt cgcaaattca atacaaccaa tgcgactcta
    13621 atatttatgg cttgattaaa gacaacacga acatggtagg ctttgcaaag tttaatcctt
    13681 acactggagt ttttacaaat atcagttcgc tcaactcctc cataggatat tgctttggat
    13741 gtatgacagt gatagatcct gttgcgaatt tttttgcatt tcagagtaac ggagtaattg
    13801 taatcgaaat aactaccggt aacataattt ataattcgcc attaaataat ttacccaacg
    13861 aaaatttcgg acatatagca ctgaagtgca gcacacatga aatatttggc acctcggcca
    13921 atgtaagtac aggattgaaa taccttgcta caattgatcc tgcaacggga aatgtatcgc
    13981 atgtatctgc aaacggatgg acaccgggat tgtttaaacc cggggcaggt ggcgatttta
    14041 taaatcaatt tacgggcgat tatttttact ccggtgccaa ctcaaaattg ttaggtgcaa
    14101 ataccataac aggcaatttg gtatacaaca aaactatagg caatcaggga gaaatacttt
    14161 ttataaatca tttttcatcg tgtggttgtg gcagcccttc ggcaataaat aatgttgcac
    14221 ctcaaaataa tactattgaa attttgccca acccattttc gaattatatc aatgtaaaaa
    14281 atatcgttac aaaccaaaag gtatattttc aattgatatc tccattagga aaaatagttt
    14341 tcgcttctat aattaattca agtgaaccaa taaatgtatc gcaattaaat ttgcctcaag
    14401 gaatttatca ttacagaata tttgatgagc aacaaagtaa ggttctgcaa accggaaaca
    14461 ttatgcacac acaggattaa tattatcttt tttaaaacca attattttct atcacaaaat
    14521 tttaatctat ttggctaatg ctggatggcc ttttatggtt tgaggaaact tttctcaact
    14581 aattccgttg gctaaagccg aacggcaatg aatgcttcgc gatagctttg tgctaattat
    14641 gaactattaa accatgaact tatgaacttt cccccaacta ctctttcaca aacttcttac
    14701 tcagcacttc cttctccgtt tgtaaatgca ctacatacaa gccatcactc caacccgcac
    14761 aatccacatc caaagtaaaa tatcctgcat tactcttcaa acttataact tcaaacttca
    14821 aactccctcg cccatcgtat atactcactg ttacattctt cccttttata ttatttgccg
    14881 ttaccactaa ttgttgtgtt aatgtattat aaatggcata gagttgttgc tccccccttt
    14941 ggggggcggg ggggctgttt gcactcaacg tatcgcaagg actccccacc attgccccaa
    15001 tctcataatg cggtatattt ggcatggtac tacttatagg gtaaataaag gtaatagcgg
    15061 tatcatcata gttacaagca aaacccgggc tatcgggttg gtgtattaca tgcaaccatt
    15121 gtgaacctcc attgtttatg tcaatgtaca atttattatc tggccctaac tgcattctgc
    15181 tatttgattc aattggtgct atcacactta gcgaatctat aacaataccc gatgcggcaa
    15241 tatttggcgc aagcatatca aattgacgta gtttcattgt ggtgcttaca tacaagtact
    15301 gcccacttgg cgaaaactcg caaccaccat ttacatgaaa ccaattaaaa ctattatcat
    15361 aaaaatgcaa agtaatgggg ttagatgcaa ggccggtaca cctatcaaaa tcataaatat
    15421 caaaccactg ttcattaaca aggtgccaag cattgctgca agcaatttta ttggtgcctg
    15481 gagcaaactt aaaatttaca aagccagcat aatttactgg tccaatattt tgataatatg
    15541 ggccatgcac accactcggg tcaaccaaaa atcgaataaa ctctggcttg taaacatgcc
    15601 ttacaactat ccaccaatcg cgcccattgc catgttttat ggcacataca ccggaaccaa
    15661 ttgtgtcatt taaaattaca ttatttatgg tggaagcaaa cccatttaac ccatttatag
    15721 ttttattcac cttggcataa taaaatttgt tgggcaagta atcaccacca ccgtttgggt
    15781 aaaaataaat aatatcaccc ccagcataaa ataaataaaa ctcgttggta tcgccagggt
    15841 cgggtataaa tacatccatc tgccataaat taggtcccga attataagtt acacttgtgt
    15901 aatagcttgg gcataaagta tcgtcattca ttattttcgt atgttgacta ttccaaattg
    15961 tgtagccatt ggtataatac aataaattac ctgatgagtc accaatcgaa gcatttgaat
    16021 aaaataattg gtgatacact ccattgacat ttgaagtact aacattggga aaaaaattca
    16081 actggttatt atttggaaat ccaaacatta tccaactatt aggtgcttgc gcttttgcaa
    16141 tatagcaaaa aaccaccatg ataacaatca tggtggttaa tttaaaaatt agcttcatcg
    16201 ggcaattaca attttggcag tagctggtaa aagatttttt gtcattatca taagccatac
    16261 catcttgcaa agacaaatca acaaaagtta ctacatatgg atttttcatt ctattctttt
    16321 atgaacttct tactcaacac ctccttctct gtttgtaaat gtacaacata caatccactt
    16381 gcccaaccgg tacaattaaa atccaaatta aaatatcccg aataaacttt cccaacttca
    16441 aacttcccct caactttcaa actaaaattt atacttttgc tacatgctta cacaatcgaa
    16501 aaagaaaaca tcaataggcg aagacacact gctaagtgct tttgaaaaaa tgtgcactgc
    16561 caaagcgatg acagaaatat atgaagccaa tgcgcagttc acctcgaaat atgtacatgc
    16621 tacatcacgt gggcacgagg cagtgcaaat tgctttagga ttacaattgc agccacaaga
    16681 ttatttatcg gcatactatc gtgacgattc tattttattg agtattggct tgcaacctta
    16741 tgaactcatg ttgcaattaa tggccaaacg cgatgatcct tttagcggag gtcgcaccta
    16801 ttattgccac ccaagtttgc gcagagaagg tatgccaacc ataccacatc aaagcagtgc
    16861 aacaggcatg caggctattc caactacagg tattgccatg ggaattcagt acaaagaatt
    16921 gcaaggcata aatgaatgga cagcactcga tgcgcccatt gcagtgtgtt cgttgggcga
    16981 tgcatcagta accgaaggcg aagtgagcga agcttttcaa atggcggtac taaaacaatt
    17041 gcccatttta tattttgtgc aggataatgg ttgggatatt tcggccacag caaaagaaac
    17101 gcgtgcaatg gatgccaacg aatatgcaca ggggtttaaa ggaatggaag tgagcagtat
    17161 agatggtacc gattttacaa cgtgcttcga aacaatacaa gatgtaattt caataatgcg
    17221 tgccgaacgc aggccgttta tgattcatgc taaagtgccg ttattgaatc accatacatc
    17281 gggcgtgcgc aaagaatggt accgcgatga tttggaagaa gccgcacaac gcgatccatt
    17341 tccaaaactg cgcaagcttt tattgcaaag tggaatagat gaatcgaaat tgatactcat
    17401 agaaaacgat gcccgtgcat tagttgaagc cgattttata aaatcaaaaa atgccgaaga
    17461 cccacgtccc gaagatttat ttttgcatga atttgcaccc acaactatta cagaagagcg
    17521 tggcatacgc caacccgaag gcaaagaaaa aaccgtaatg gtggatagtg ctttgtttgc
    17581 tatacaagag ctcatggcaa agcatgccga gtgtttatta tatggacaag atgtaggacg
    17641 caggctgggc ggggtttttc gtgaggccgc aacacttgca caaaagtttg gcgaccaccg
    17701 tgtgttcaac actcctatac aggaagcatt tattattggc tctactgtgg gcatgagtgc
    17761 cgcaggttta aaaccaatag tagaagtaca atttgcagat tatatatggc cgggcctcaa
    17821 tcaattattt accgaagtaa gtcgctcgtg ttatcttacc aatggaaaat tcccggtgag
    17881 ttgtatcgta cgtgtgccca ttggtgcata cggtagtggc gggccatatc actcatcgag
    17941 tgtagaaagt attcttacaa atattaaagg cataaaagtg gtatatccca gtaccggtgc
    18001 cgatttgaag ggattgatga aagccgctta ttatgatcct aatccggtgg tgatgctcga
    18061 acataaaggt ttgtattgga gtaaaataaa aggcaccgaa gatgcaaaaa caatagagcc
    18121 tgacgaagag tatattattc cgcttggtaa agcacgcaca gttattattg ccgatgacga
    18181 taaaataaaa tcaagcgaaa gcatggtaat aatcacttac ggcatgggtg tatactggag
    18241 taaaaatgca gcaaaaaact ttgaaggcaa aatagaaatt attgatctgc gtacattata
    18301 tccgctcgat gaagttgcga ttttcgatgc agtaaaaaag caccaccgtg cacttattgt
    18361 taccgaagag ccctacaata attcgtttgc acaagcactt tgtggacgta tacaacagca
    18421 ttgttttcaa tacctcgatg ctccggtaat ggtaataggt tccgaaaatt tacccgctat
    18481 accactcaac acaacactgg aatttacaat gattccaaac acagaaaaag taggagtgat
    18541 gattgaaaag ctgttggctt attagcatta atttgattgc gcatagtgtg atgttaaaag
    18601 ctgttctgtt ttataaaaat cacaaaaagt aaaattcgaa aaaataggat gtgcatcaaa
    18661 gtttaatttt ttagatgcac caatatcagc atttaaaacc cttaatgcat ttttgccctt
    18721 gattggtatc gccaattgcg agacaagccg ccttgatttt attaaaattt tttattccat
    18781 tttttattca ataataaatt tcttactcaa cacttccctt tccgtttcta aatgtaccac
    18841 atacactcca cccgcccaac ctgagcaatc caaatccaaa gtaaagtaac catttacagc
    18901 cttcaaactt tgaacttcaa acattaaact tcccctccca tcgtatatgg ttattgttac
    18961 attcttccct ttcaatccac tcgcatttac aaaaagcttc tcccaagccg ctacgtatgt
    19021 aatttgcatt actccctctc ctttggagag ggccggggtg aggagagtta acgtatcaca
    19081 aggactcccc tttaacggcc ccaaatcata atccggatta tttggcaaac cccaatacgt
    19141 acgtgcacca ccaagataaa aactataagg tgtaaagtta catgcagcac caaggctatc
    19201 gggtgagttt attacactta ggttcatgtt gtacatatta aatgaggccg gagtatttgg
    19261 ataaggataa ttccatgtgc ctaaactatc tatccatgca cacgaccaat atattttatc
    19321 atcggggcca cgtttaagtt gaccacccgg agattccagt ccagtaatac tatcaatagt
    19381 atcggccgtt gcatataata atggatttag caaatctact tgaattaaat atgcggcatt
    19441 ataataccca cttgttgcaa tgtataaaaa tctaccgttt ggggagaagt cgcaactcca
    19501 gaattctgga attggcggtg gcacaggtgt aggtggccta accgatacat tatttgatag
    19561 ttgacctgtg catctatcaa aatcgaacgt ttcacataaa ccattgtagt agaccataac
    19621 taatttattt cccgttttat caaatgagta tttaataaac ccaatatttg taatgctgcc
    19681 tatattttga gtatcgttta ataaaactcc atttttatca attaaatatt tgaagtaggt
    19741 gttgttaaga ttggttgtaa caccacgaaa aagcaaccac caatctctac cattgccatg
    19801 ctgaatagcg gtaaggccat cgttggcttt catgttattt acttgtatgt ttttttgtgt
    19861 tactgcacct aagccgttgt ttaatgtcat gtctattatt gagtagtaaa gtccataagg
    19921 cgaactatta gaaactccaa ttgaaaaaag ataatactta tttgtatcgg atggaaatgg
    19981 aactattata agttcataat accaaccttg acctaataca tttgttccat tttgcattgg
    20041 attgttattt cggtctttca ctaaagttgt attgccagcc aatcctgcgc gtgtataagc
    20101 ataaaacaaa acatttgaat ttgtgtcaca tattgaaacg caactacctc tagattttac
    20161 agatgtaata aaagttgatg gtgaatttgg atttacaaaa ttgattcctg cactatcacc
    20221 aaaacaccat atattatttt taaattggct ctgaccatta aaagtaaaaa acagaaatat
    20281 taataacgta aaaattattt tcatttatat gccttaataa atctcgtgct caaaacttta
    20341 ttcttcgttt gtaaatgtac aacaaacatc ctattgctca aaccaccaca atctacaccc
    20401 aaagtaaaat atcctgcatt aactcttaaa ttcacatggc tagagccgaa cggcaatgaa
    20461 tgcttcgcgg aaaattaaat gcagccctgc ccctaccaag ggttttaatt caagaaaact
    20521 ttctaaacat tacaactatt aaacttttga actattaaaa tcacgttggc taaagccgaa
    20581 cgcaataaaa gcttcgcgga aacttccccc aactattaaa ctcttgaacc ctccccaact
    20641 cttgaacctc cccaactctt gaatgccgaa accctggtcg gcatcccgac cgaagtgtcg
    20701 tggctttcaa aactttccca accttcccac cttttaattt acatttgcaa caaataaatc
    20761 acacacactt tgtttcgaaa taaattttat aaaagaaata agccaggcag cattttcaaa
    20821 ttttgggtaa tagccatttt aattttgtgc gtgcagcaaa acattttctg ccagtcattt
    20881 c
//
LOCUS       DAORQA010000023        20571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_667_length_20571_cov_28.157146, whole genome shotgun sequence.
ACCESSION   DAORQA010000023 DAORQA010000000
VERSION     DAORQA010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 20571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20571
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_667_length_20571_cov_28.157146"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="PK736_03215"
     CDS             <1..1350
                     /locus_tag="PK736_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium symporter"
                     /protein_id="HRC32434.1"
                     /translation="FGGKHGHHSTPFILGSMDKRKFWKYIGVFGIFTNLAVAAYYCYL
                     ESWALSWAYHSVTGTFAGKGQDDVAAIFGNYVSLTNAEPIIFWAICLLMNTYILSKGL
                     SGGVEKVAKIGMPLLILFGVFLAIKGVTLHAGEQGAVYDGTVGLNFLWTPDFSTIWSA
                     KVWLAAAGQIFFTLSVGMGSIQCYASYVKPKDDIALNAMSAGWMNEFVEVVLGSCIII
                     PISIGYLGIDKVVEMTKTGGLGLGFKTLPYLFQQWGSTMSVIAGLMWFGLLFFAGITS
                     SLAMGTPCMGFMQDEYGWSREKSAWSFGAVVLILGLPTVLFFNLGVFDEYDYWAGTVS
                     LVIFALIEIILFGWVFGMEKGWLEITDGADIKVPRIMIYLIKFVTPILLGFVFFMSLD
                     EIITKATDFSSTPVVLSRLLLLGLWIGIALLVRNAYYKRIREGRFLNMDDIKSKNKK"
     gene            1356..1502
                     /locus_tag="PK736_03220"
     CDS             1356..1502
                     /locus_tag="PK736_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32435.1"
                     /translation="MNTTALIMMITSLSVVTLVTAYYFYHAFTTPPKPIDDGHQDINR
                     PDFT"
     gene            1564..1986
                     /gene="tsaE"
                     /locus_tag="PK736_03225"
     CDS             1564..1986
                     /gene="tsaE"
                     /locus_tag="PK736_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRC32436.1"
                     /translation="MKEFDMTLLHDLPRAAIFIIEHLQQSKKNIVAFYGTMGAGKTTL
                     IKEICNQLNVTSNTSSPTFSIVNEYVCNYGRTIYHFDCYRIEKQNEFFDLGYEEYFYD
                     NSSLCFIEWPEKIEALLPVDRVNVYLQIVDGKRMLRIA"
     gene            2105..6664
                     /locus_tag="PK736_03230"
     CDS             2105..6664
                     /locus_tag="PK736_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32437.1"
                     /translation="MKNKIYPVILLFVLILFATSNLTAQQQVFPLPSVNGTVYCIAED
                     SSRVYVGGDFTEAGVGVNGNVMCDTSAQANIIFDSLTIGLSGIISSVIPDGIGGFFIG
                     GNFISASGKKNLIHVDINKIEYPNFNPSPEGYSYYEIVSALHLINDTLYVGGDFYKVA
                     NNTINYLAALNASTGLAYNFMPLVDGAVKDLKQVGDTLYFGGQFSHINNTSQVGIGAI
                     KISTGQLIPMPVSVAGNVYKIFYNPSDNRIYMCGNIIGIGANKSSITEFNAQTSAINN
                     NFPKFEGLKYLFTSTPDGVGGWYIGGMFMLNNGIKNLMHILNNDMLDTAFHPNPNKTV
                     RALSKLNNILFVGGEFDSIGGIARKGIASINANSGIVNNWNAKCDGTVNAILAKDSLL
                     YVCGFFYYITDKRLPGFAILNINNFKIYSDYTFVGSINSITADTNAVFVAGNFNSYGK
                     SILNFAKVTTTNVTIDKNMALLQRDYFFEDIYNSIFSAASDGIGGWYLCGNISTKQGL
                     KNLVHINANNTLDTVFNPNPDNTVYDLHIKGNTIYVAGNFTNVDGQSQPYLAAVNKTN
                     GSLINSFTPQINSNVKCIEVVNNDLYLGGDFTTINTVQRSRLASINIGTGILSTWNPN
                     VDKSVYCLTIKDSVIFVGGTFKFVGTQYRNFCASIYLSNGLPTPWNANINSQYYVVYD
                     INITGNKIYVGGTFDKVNGLNRKNLAAYDLASGNLINGFNAKLDGQSVNTITSVGNKI
                     FIGGNFTTAFSQPRKNAACFNTSNILQNWDPSFGNYNSNILVYKIAAQGNNLLVVGYI
                     KIANVIQKNSVAKINLITNEIEPWSITFNLNYGIKCMTLNGQNLYIGGQFDSCNSQAR
                     KNAAAVNINTGLLQPWNPNVYSYRVDALMSKNNTIFIGGVFNKVGGKIKGGLAAVDNN
                     LGLLTSNQLSTNGSVYTLSSFSNDVLACGEFSVSNVLETGSICAFNHNNWTVPKWDPK
                     LQDGGEVYEMFLAGNLIYACGNYTSIGGQTRHGLGAINVNTGNATPWNPNPDSTVFSI
                     AKYGTKIFACGVFQKMNGVPQKYTAALKVSNGSIDPWQVEPNRSIKKLRVLGNKIWLS
                     NYGSFMLIKTQKRNGLFSYSKSNGIIDSTFHPAFSGFLQSNSSTAIRAIMIHNDSIFI
                     GGNFQYVDGMQRNGLVALQKTNGNLLPFQASFNNSVKCIKYYKDKLYVGGLFNKLNGV
                     YQFGLCTFNYNTGALLPFNPMSWGSIFDILVADSTLYVGGQIYDAGGIPRKNLAAFDI
                     NTNALRAWNPAANYLVRTILNDGNNIFIGGDFTTIGGNVRNHIASVDKNLGALNSWNF
                     SLNSSCNSIIRKGNELFVAGDFNVVNGDTIGPIFSVNSFTGKIGDYKPKIQHWQNGNS
                     FSNFISVNAISFADYTDAQLKIGGSFNLVNNKQSGGLTSFSYFAPPSPNEKINLNSNS
                     KNITQNILSCYPNPANEKLYVTTLSNNPFTVSVKNISGAELIHKNINGGNLVYELDIT
                     SLNSGIYFITYTDGKVKSQSKFVKQ"
     gene            6773..8002
                     /locus_tag="PK736_03235"
     CDS             6773..8002
                     /locus_tag="PK736_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRC32438.1"
                     /translation="MNINISPAGMSALAQSGAFSTQESLLEVRRQKKELFIGIPKESS
                     FQENRISLVPESVALLINNGHRIRIEAGAGSNATFTDNDYSEAGAEIVYSPAEVYESD
                     IILKVAPLSTEELSLTKEKQTIISIVQAHSQNKEYFKHLSQKKITGIGFEYIKDQTDL
                     IPVIRAMSEIVGSTAILIAAEYLTKDNNGQGIILGGITGIPPTEIVILGAGTVGEYAA
                     RAAIGLGANVKVFDSSVYRLKRLQNNIGRRVYTSTINPRLLRETLHTADVAIGAIYST
                     DGRTPCIVSEEMVSEMRYGSVIVDVSIDQGGCFETSEVTNHNKPTFRKYGVIHYCVPN
                     IASRVSRTASYALSNIFTPILSSMADAGGMEELLWQDVNVRHGVYMFKGTSTNKFISD
                     YFNLPFRDIDLIMASRI"
     gene            complement(8429..9907)
                     /locus_tag="PK736_03240"
     CDS             complement(8429..9907)
                     /locus_tag="PK736_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRC32439.1"
                     /translation="MSIIKKLASQSVWYGLSTMVGRFLNYLLTPLLTYTFLPPEYGII
                     SYIYAIISFANILFTYGMETTYFYFGKQQNEKKIFDNTFSMMLLSTVIFSSAIILFRN
                     PIQHLFKIEFGAHYIIMMAALLFFDTLSVIPFAHLRSTAKAKKFAYIKLTGIITTVLL
                     TLFFIKVIPGWYQSSNSMLHNFSALIYSPPLTVSVVFIANIIGSIVVLLFLYKEFAQV
                     KLGIDKALTKQMLWYSLPLLLAGFAGMINETLDRIILTYLAEPASSAMHQQGVYGAVY
                     KISILMTIFIQTFRYAAEPLFFAQSNASNSKENFARVMKYFSITCLFIFLFVILNMSW
                     IKYFVGKNYWEGLHVVPILLAANLCLGIYLNQSMWYKLSGQTKFGAYISIVGAVITIV
                     LNIILIPQIGYTGAAITTFITYASMMIISYIMSLKYYPINYDLKTMGVFLLLTIAIYA
                     ASVFIQSQNIASNGVILAVNELMLGLFLFVVYRREVRGHWSL"
     gene            complement(9945..10718)
                     /locus_tag="PK736_03245"
     CDS             complement(9945..10718)
                     /locus_tag="PK736_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC32440.1"
                     /translation="MSYANIVVQNENRITTITVNRPEKLNALNQATIDELGQAITACQ
                     SDAETAVVILTGAGEKAFVAGADISAFSTLSAEQGSALSQQGHAVFNMFENSAKPVIA
                     AINSFALGGGCELAMACHIRVASETAKFGQPEVKLGLIPGYGGTQRLVHLVGKPKAIE
                     LLITADNLNATDALQWGLVNHVVPAAELMNKCKEIAEKIKQQAPLAVSASIACANAAN
                     TSGGFDMEIAQFGKCFATSDMKEGVDAFMNKRKATFKGE"
     gene            complement(10743..11147)
                     /locus_tag="PK736_03250"
     CDS             complement(10743..11147)
                     /locus_tag="PK736_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRC32441.1"
                     /translation="MRPIRVLVAKVGLDGHDRGAKVIASFLRDAGMEVIYTGLRQTPE
                     MVVNAAIQEDADVIGVSILSGAHMTVFPKILNLLKEKNATDILITGGGIIPDGDIKKL
                     NDLGIGKLFQPGTNTDEIVAYITNWVKQHRNF"
     gene            complement(11177..12613)
                     /locus_tag="PK736_03255"
     CDS             complement(11177..12613)
                     /locus_tag="PK736_03255"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRC32442.1"
                     /translation="MKSVFFAFRNLILCSIIICFCSNVSLAQIVWHLNDSLHPTVYVA
                     NITITGNKKTKEAIIVRELIFHKNDTLNTDVLFAAAQRSMENVMNTSLFNFVEIHVTP
                     QDITGADITIIVKEKWYVWPFPIFEGVDRNINEWWQTKDFSRVNYGMYLLHENFRGRK
                     ENLQINARFGYSLKLGLTYNVPYIDKAKTQGLAFSAGTQRYRETAYALAGNQLKFYTN
                     SQSVVRRETYAWLRYTKRKGIYRTQNIFAEYRDVKLDDSLSTLNPNYLKSSDNRIKMI
                     LAGVSYRYEQVDFNVYPLIGYAISGEVTQNGLELLHNEPAILSATLGARRFIKLSPRW
                     YHAISVRAKVFSSPTVPFYLQRGLGYGNDFVRGYELYVVNGQYHFVAKNNFKFALLPL
                     RKIKLNFIRTEKFNTIPIAIYINMFADAGYARSKYNNENNTLNNNILSGYGVGIDVVS
                     YYNLVWRFEYAFTNIGKHGFFVHFSSPI"
     gene            complement(12776..13924)
                     /locus_tag="PK736_03260"
     CDS             complement(12776..13924)
                     /locus_tag="PK736_03260"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020597396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRC32443.1"
                     /translation="MKKSIAILGSTGSIGTQAIEVCMAQSDNFDIEVLTANSNHELLI
                     AQALQVKPNTVVIADTTNYALVKDALEPHDIKVFAGNNAIAEVVQMDNIDIVLTAMVG
                     YSGLLPTINAIKAGKSIALANKETLVVAGALINELAQKHGVNIYPVDSEHSAIFQCLI
                     GEWHNKIEKIYLTASGGPFRGKDISFLESATPAMALKHPNWRMGAKITIDSASMMNKG
                     LEMIEAQWLFHLKPEQIDVIVHPQSIIHSIVQFEDGSMKAQMGLPDMKLPILFALGYP
                     NRLKTDFPRFNFINYPQLTFETVDKKVFKNLAHAEYAMHKSGNMPCILNAANEIAVEA
                     FLKEKIKFTAMPNIVEACMNKINFIANPTYDDYVQTDISTREFANTLV"
     gene            14244..19349
                     /locus_tag="PK736_03265"
     CDS             14244..19349
                     /locus_tag="PK736_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32444.1"
                     /translation="MKNSNSTIQQKNNPKDSNFDKHKLFVIHLLLILFIGSMANAQSI
                     KQIGPGAGGQERAVYIYPNPVGSYDLYVGSDVSGVWRATNIPANGFGNVYNYTYNFIS
                     NHEIMRFVNKFYRADMLATPYLFCLNRSGIDRIDLTNYNSSMSTVPVFENSAEITMED
                     SWVSDMYVNATSKQTYFITGNTRVTDDGPGNFKGTNVSDIFTGTLTSTYNRVDINGKK
                     ALFNLTTNNRDVYCLYVDDQSNTDATDDIIFAGAENGLHIITHNELAGTASLLPTTQK
                     AGPSTNQLENKVTSLFLVSKTTQGNNTMYSFVATVHGMGLYRLDCVRNTSNLIFTTIT
                     WTLLSTNVTGINQGTPQGCSQSEPYLIPKFTKCLPVFNGQNIAGYYITNEINLEKLCT
                     GRQANYFTGVYYCPADIDGFPDAGGLKPLTVWQGGNDWGWNATKPAANINGALITPEN
                     TLLIGKQGNIFVTSNDNATPDENVHWHQIYDLQSTVANCYTENQFIHRGYINTANKTI
                     YPSTKTDVWLGQADRLIFRDNNSNDYYHSIDATNTCYTFNYTGQSCGNNLGKNDCWFI
                     TNNTIDGEDQNIYIGIGEGYASQVGCGHVMFGDKDGNTFNEIGDPSINGDPRKILFTS
                     DGNKFLLVNKKISVNNHKLRLFTLVNNIWTEIDLSTVNNYAVSDAVISPDGKNIFAII
                     KEVNDVHFYAFPFINGILGTPCINSVGTSAVTPVRLSMLRYGAHLENYKILCGTVPII
                     DGTTTDNFFEVYNSALPNTCIPNSINPSNGLSLIFKNLHKDVLDNTDEGISAIAVNEK
                     MNTLFVATVTGNTYDFVSPVATKSHLYKASYNFLTGVIDNNSWTDITSTLPNKTIIDI
                     ATHGSSTCDEYLYLCLRGLGGWQLALPAAVSINNAFNSIAPITGYGLLTTSVTLGPNA
                     NIVTIDGANIGIGPGKTITVPSGKTLKIINGSHLYACGDMWQGIVVDAGGTLIVDGSI
                     IEDAVLAIINNGLKLTVINSAFEHNYVSITLNDQNFSTTEIYGNKFSCTSGQISKIPY
                     NGQQTRYHISVGGVDGLTIGNSALNKNIFQNASKAIFVTNSNVDIINNQFEWIPNNLN
                     TWGEAIYIDGKPTISTIRKIGGNVNANDKNTFINWKQNITVSNAGKILIENNVINPDP
                     ANRSFVGISQQKCTDVTIKGNAINRSNNGIKLFDNPGPTVTVTLNTFNKNFAYDFTKY
                     DDEAIRFENNLDMKTKLYTITNNEIYNVAVGLHGMNTNSTLDYNTYNTHITLNDLTTY
                     YTTHNSQPHFGIWMQNASGSALRNNVIEWNGNAFITSDELTKLHAIRMQGSGSCVLQE
                     NTMTRFGGGIDIFDDCSKTQLECNTMDGCYHGVYFDINGPNNKLTDQGVAVFDPLYNA
                     ALSKTWHNHWIGNVGTNKVDGQYFNAFFNWMYFDNPFDAEYAPIPAIPLVFARPCSLN
                     TSICAAPIVNDDHLRMAAYGAAVGDSSYADYDSTEYAHSDKSTFYKAAKIDSAVLYLG
                     LPSDAIYQAEFNSLKQTDIGLFEDAKDAINNNNSSLAFQKLSAMVKQNTIDENNNYMT
                     MLVALNYNASLDADSDTIVVVNNIAMMHPFYGGEAVYIARAMLHLNIADVLPPLRRQH
                     KNVESLVIVNTNFSLIPNPATTEVSIFSAQSFINTKLQLYDSYGSLVSNIPLPNKTNS
                     FTFDISNLVGGIYFTYLKSDLGISQMTKLVIIK"
     gene            19481..>20571
                     /locus_tag="PK736_03270"
     CDS             19481..>20571
                     /locus_tag="PK736_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32445.1"
                     /translation="MIWCFGDSAGIDFNNLTIPTPIATGGDGRGNCTSIADSNGNLLF
                     YATENSNSDAVIYNKNHTLMANTSGMPSDGYFAGMVIIPKPGSDSLYYLFYRGNPNNV
                     DTFYRAIIDMSQNNGLGAVIEKNKVIQPISFADCITAVKHGNGRDWWVIDKYYVTPFT
                     HHNRFFAYLIKPDTIMPAIIQDFNDMNDGAFQKICWHPSFNKFMLISIVGYMAEFDFD
                     RCTGAISLNRIIFPEQLSNYNRIFWEGAYSPNGNVFYVSRNSYGGNFGNYNYLLQYDL
                     TATNIPASCDTLNFTSYPSADCGAVRLAPDGKIYYTHAYIPTNALNYPYADTMRNYIN
                     ENLGVVNNPDVVGPGCNFAPFSFHLGGKRT"
BASE COUNT         6606 a         3662 c         3845 g         6458 t
ORIGIN      
        1 tttgggggga agcatggcca tcattccaca ccatttattt tagggtctat ggacaagcgc
       61 aaattttgga agtacattgg cgtgtttggc atttttacaa atcttgccgt agcagcatat
      121 tactgttacc tcgaaagctg ggctttatcg tgggcatatc attcggtaac cggcacattt
      181 gccggcaaag gtcaagatga tgttgctgcc atttttggta attatgtttc gcttaccaat
      241 gccgaaccaa ttattttttg ggcaatatgt ttgttgatga atacttatat tctgagtaaa
      301 ggattgagtg gaggtgtaga aaaagttgca aaaatcggga tgccattact catattattt
      361 ggagtgtttt tggcaataaa aggtgtgact ttgcatgccg gtgaacaagg tgctgtgtat
      421 gatggaactg taggcttaaa cttcttatgg acgcccgatt tttcaaccat atggtcggcc
      481 aaagtttggc ttgccgctgc cgggcaaata tttttcactc tttctgtagg tatgggttct
      541 atacaatgtt atgcctcgta tgtaaaacca aaagatgata tagccctcaa tgcaatgagt
      601 gccggatgga tgaacgaatt tgttgaagtt gtgcttggaa gttgcatcat cattcctatt
      661 tcgattggat atttaggaat agataaagtg gtagaaatga caaaaaccgg aggacttggc
      721 ttaggtttta aaacactgcc gtatttgttt caacaatggg gaagtacaat gtcggttata
      781 gcggggctta tgtggtttgg acttttattt tttgccggta taacatcttc gcttgcaatg
      841 ggcacaccat gtatgggatt tatgcaggac gaatacggct ggagtcgcga aaaatctgcc
      901 tggtcatttg gtgctgttgt tttaatttta ggattgccta cagttttatt ttttaatctc
      961 ggtgtgtttg acgaatacga ttattgggca ggtactgtct cgcttgtaat atttgcattg
     1021 atagaaatta ttttattcgg ttgggtattt ggaatggaaa aaggatggct cgaaattacc
     1081 gatggtgccg atatcaaagt gccgcgcatt atgatttacc tcattaaatt tgttactcct
     1141 attttattgg gctttgtatt ttttatgagc ctcgatgaaa taattaccaa ggccaccgac
     1201 ttctcatcaa ctcctgttgt attatctcgt ttacttttgc ttgggctttg gattggcatt
     1261 gctctgcttg ttcgcaacgc atattacaag cgcattcgcg aaggacgttt cttaaatatg
     1321 gatgacatta aatcgaaaaa taaaaaataa taaatatgaa tactacagca ttaataatga
     1381 tgataacatc acttagcgtt gttacgcttg taactgcata ttatttttac catgcattta
     1441 ccacaccgcc aaaacctatc gatgacgggc atcaggatat taatcgtccc gattttacgt
     1501 gatacttttt tgaaaaaatt tatagttgac atcttaacgt tagcaatata attttctaca
     1561 tcgatgaaag aatttgacat gacattgctg cacgacttac ctcgtgcagc aatttttatt
     1621 attgagcact tgcaacagtc caaaaaaaat attgttgctt tttatggcac aatgggcgca
     1681 ggcaaaacaa cattgataaa agaaatctgt aatcaattga atgtaacctc taacacatcg
     1741 agcccaacgt tttctatcgt taatgaatat gtgtgtaact atggccgcac catttatcat
     1801 ttcgattgtt accgcattga aaagcaaaac gaatttttcg acctcggtta cgaagaatat
     1861 ttttacgaca acagttcatt gtgttttatt gaatggcccg aaaaaattga agcccttttg
     1921 ccggtcgaca gagtgaatgt ttatttgcaa atagtggatg ggaaaaggat gttgcgcata
     1981 gcataatatt tcccatactt tttttgttgt ttcagatttc tttgcaatgt atatttcttg
     2041 ttgcttttgt gtggcttttt ttttaatttt acatgtgtta ataaactcct caaaatcaaa
     2101 tgccatgaaa aataaaatct accccgtcat tcttcttttt gttttaatac tatttgccac
     2161 ttcaaattta actgcacaac aacaggtgtt tccattgcca tctgtgaatg gcactgtata
     2221 ttgtattgca gaagacagca gtcgtgttta tgtgggaggc gactttacag aagcaggtgt
     2281 tggtgtaaat ggcaatgtaa tgtgtgatac ttctgcgcaa gctaatataa tattcgattc
     2341 tttaactatc ggattaagtg gtattatttc ttcggtaatt ccagatggga taggaggttt
     2401 ttttattggt ggcaatttca tttctgcaag tggaaaaaaa aatttgattc atgttgacat
     2461 taataaaatt gaatatccga attttaatcc ttcacctgag ggttattcat attatgaaat
     2521 agtatctgct ctgcatttaa ttaatgatac cctttatgtt ggaggtgatt tttacaaggt
     2581 cgcaaacaac acaattaatt accttgctgc cttaaatgcg agcaccggct tggcttacaa
     2641 ttttatgccg ctggtagatg gagcggttaa ggacttaaag caagtgggag ataccctata
     2701 ttttggtggc caattcagcc atataaataa tacttcacag gttggaatag gggcaattaa
     2761 aattagcaca ggacaattga tacccatgcc ggtttctgtg gcaggtaatg tatataaaat
     2821 tttttataac ccatccgaca atagaatcta catgtgtggc aatattatag gtataggtgc
     2881 aaacaaaagc agcattacag aatttaatgc acaaactagt gctatcaata ataatttccc
     2941 caagttcgaa gggttaaagt atttatttac aagtactcct gacggtgtag gtggttggta
     3001 tattggcggc atgtttatgc ttaacaatgg aataaaaaat ctaatgcata tcttaaataa
     3061 cgatatgctt gatacggctt ttcatcccaa tccaaataaa actgtaagag cgctatcaaa
     3121 attaaacaac attttatttg tgggtggcga atttgattca attggaggaa ttgcacgaaa
     3181 gggtattgcc tcaataaatg caaacagcgg aattgtgaat aattggaatg caaaatgtga
     3241 tggcacagta aatgcaattc tcgcaaaaga ttccttactt tatgtttgtg gattttttta
     3301 ctacattacc gataagcggt taccgggttt tgcaatactt aacataaata attttaaaat
     3361 ttatagtgat tacacctttg ttggcagtat caacagcata actgctgaca ccaatgctgt
     3421 gtttgttgcc ggcaatttta attcttatgg aaaatcgata ttgaattttg ctaaagtaac
     3481 cacaaccaat gtaacaattg ataaaaatat ggctttgcta caacgtgatt acttttttga
     3541 ggatatttat aacagtatat tttcagctgc aagcgatggt ataggtggtt ggtatctttg
     3601 cggtaatatc tccactaaac aaggattaaa aaacctggtt catattaatg caaacaatac
     3661 tttagatact gtttttaacc ccaaccctga taatacagtt tacgatttgc atattaaagg
     3721 aaataccatt tatgtggctg gtaattttac aaatgttgat gggcaatcgc agccgtatct
     3781 tgcagccgta aacaaaacaa atggttcact catcaacagc tttacacctc agattaacag
     3841 taatgtaaag tgtattgagg ttgtgaataa tgatttgtat ctcggtggtg attttacaac
     3901 tataaacaca gttcagcgtt cacggttggc ttctatcaac attggtacag gcatattgtc
     3961 aacctggaat ccaaatgtag acaaatcggt atactgccta acaattaagg actctgttat
     4021 ttttgtgggc ggaactttca aatttgttgg gactcaatat cgaaattttt gtgcttcaat
     4081 atacttaagc aatgggttgc ctaccccttg gaacgcaaat attaattctc agtattatgt
     4141 agtatatgac attaacataa caggtaataa gatttatgtg ggaggcacct ttgataaggt
     4201 aaatggtctg aatagaaaaa atcttgctgc atatgatctc gcatcgggta acttaataaa
     4261 tggttttaat gctaaactgg atggccaaag tgttaacaca attacttcag tgggcaacaa
     4321 aatatttatt ggtggaaatt tcaccactgc attttcgcaa ccaagaaaaa atgctgcatg
     4381 tttcaacaca tcaaatattt tgcaaaattg ggatccgtct tttggaaact acaatagcaa
     4441 tattttagtt tataaaattg ccgcacaagg caacaattta ttagttgttg gatatattaa
     4501 aatcgccaat gttatacaaa agaattcagt agcaaaaatt aatttaataa ccaatgaaat
     4561 agagccttgg tctataacat ttaacttaaa ctatggcata aaatgcatga ccttgaatgg
     4621 acaaaattta tatattggtg gtcaattcga ctcctgtaat tctcaagcac ggaaaaatgc
     4681 tgcagccgta aatattaaca ccggattatt acaaccgtgg aaccctaatg tttattcata
     4741 ccgtgtggac gctttaatga gtaaaaacaa tactattttt attggagggg tttttaataa
     4801 ggtggggggt aaaattaaag gtggtttagc agcggtcgat aataatttag gcttactcac
     4861 atctaaccaa cttagcacaa atggttctgt atatacttta agttctttta gcaatgacgt
     4921 gctagcttgt ggagaattta gcgtttcaaa tgtattagaa accgggagca tttgtgcttt
     4981 taatcataat aactggaccg tgccaaaatg ggatcctaaa ttacaagatg gcggagaagt
     5041 atacgaaatg tttttagcag gaaatttgat ttacgcttgt gggaattata cttccattgg
     5101 agggcaaacg agacatgggc ttggagctat caatgtaaat accggcaatg ctactccttg
     5161 gaatccgaat cctgattcta ctgtatttag catagccaag tatggaacta aaattttcgc
     5221 ctgtggtgtt tttcagaaaa tgaatggtgt gccacaaaag tacactgctg cattaaaagt
     5281 aagcaatggc tcaatcgatc cctggcaagt tgaaccaaat agaagcataa aaaagcttag
     5341 agtgttggga aataaaattt ggctttctaa ctatggatcg ttcatgctta taaaaaccca
     5401 aaaacgcaat ggtttgtttt cctattcaaa atcgaacgga ataatcgaca gtactttcca
     5461 tccagcgttt tctggatttc ttcagagtaa ttcttcaact gctattaggg ctatcatgat
     5521 acataatgat tcgattttta ttggtggtaa ctttcaatat gtagatggaa tgcaacgaaa
     5581 tggtttggtt gccttacaaa aaacaaatgg caacctttta ccttttcaag ccagtttcaa
     5641 taattctgta aaatgcatta agtactacaa agataaactg tatgtaggtg gattgtttaa
     5701 taaactaaat ggagtatatc aattcggact ttgtacattt aattataata ccggtgcttt
     5761 gttacctttc aatccaatgt catggggttc tatctttgat attttagttg cggacagcac
     5821 tttgtatgtt ggtggccaaa tctacgatgc aggaggtata ccacgtaaga atcttgcagc
     5881 attcgatatt aatacaaatg cacttcgtgc ctggaacccc gcagcaaatt atttagtgcg
     5941 aacaatttta aatgatggga ataatatctt tataggtggt gattttacaa ccattggtgg
     6001 aaatgttaga aaccacattg catcggtcga taaaaatctc ggtgcgctaa attcatggaa
     6061 tttttcttta aatagtagtt gtaattcaat cattagaaaa ggcaatgaat tatttgttgc
     6121 tggcgatttc aatgttgtaa acggtgatac tataggcccc attttttcgg tcaattcatt
     6181 tacaggtaaa attggagatt acaaaccaaa aatacagcat tggcagaatg gtaactcttt
     6241 tagtaatttt atatctgtaa atgcaatatc attcgcagac tataccgatg cacaactcaa
     6301 aataggagga tcctttaatt tagtaaacaa taaacaaagc ggaggtttaa catcatttag
     6361 ttatttcgct ccaccatcgc ctaatgagaa aataaatttg aactcgaatt caaaaaatat
     6421 tactcaaaat attttgtcgt gctatcccaa ccctgcaaac gaaaaattgt atgtaaccac
     6481 acttagtaat aatccattta cagtgagtgt aaaaaacatt tcgggtgccg aattaattca
     6541 caaaaatata aatggcggaa atttggttta tgagttagat atcacttcgc tcaatagtgg
     6601 aatttatttt ataacctata ccgatggaaa ggtaaaaagc caaagcaagt ttgtaaaaca
     6661 atagtgattt agtgggcata aaaattttac taaaaataat gtaagaatat aatctggaaa
     6721 cgcttacctt catgcaccaa aaaataaaaa agcatctatt catttttcaa atatgaatat
     6781 aaatatttct ccggcaggta tgtcggcatt ggcgcaatcg ggcgcattta gcacacagga
     6841 aagtttgctc gaagttcgca gacaaaaaaa ggaattgttc ataggtattc ccaaagaatc
     6901 atcgtttcag gagaatagaa tatcgctggt gcccgaaagc gtggcattgt tgattaacaa
     6961 cggtcaccgt atacgtatag aagccggtgc gggcagcaac gctactttta ccgacaacga
     7021 ttacagcgaa gccggtgccg aaatagtgta cagccctgcc gaagtatatg aaagcgatat
     7081 cattttaaaa gtagcgccat taagcacaga agaattatcg ctcacaaaag aaaagcaaac
     7141 catcattagt attgtgcagg cacattctca aaacaaagaa tatttcaagc acctgtcaca
     7201 aaaaaaaata accggcattg ggttcgaata tataaaagac caaacggatc tcattcctgt
     7261 aattcgtgct atgagcgaaa tagttggcag cacggcaata ctaatcgcag ccgaatatct
     7321 taccaaagac aataatgggc agggaataat acttggagga ataacaggca taccacctac
     7381 cgaaatagtg atacttggtg ccggtacagt gggggaatat gctgcacgcg cagcaatagg
     7441 tttaggagca aacgtaaaag tatttgacag cagtgtttac agattaaagc ggttgcaaaa
     7501 caatatcgga agacgtgtat atacatctac cattaacccg cgattgttgc gcgaaacctt
     7561 acatactgcc gatgttgcaa ttggtgcaat atactcaacc gatggccgca cgccatgcat
     7621 tgtgagcgaa gaaatggtaa gcgaaatgcg atatggttcg gtgatagtgg atgtgagtat
     7681 agaccagggc ggatgtttcg aaaccagtga agtgaccaat cataataagc caacattccg
     7741 aaagtatggt gtgatacatt attgtgttcc caacattgca tcacgcgtat cgcgcacggc
     7801 atcatatgca cttagcaata tatttactcc catactgagc agcatggccg atgcaggtgg
     7861 tatggaagaa ttgctttggc aagatgtaaa cgtacgccat ggtgtgtata tgtttaaagg
     7921 cacaagtaca aataaattta taagcgatta tttcaatttg ccttttcgtg atatcgattt
     7981 aataatggcg agcagaattt agggaaatta aagattaaga attgaaaatt atgaatgatg
     8041 gggaaattag aaattagaaa ttgaaaatga tggggaaatt agaaattaag aattgaaaat
     8101 taggaatttt gaattcaaag taaccttata acagaataac ccattaacta aataaccaat
     8161 aaccatccca tccttccgtc attgcgaagg aggtacgact gaagcaatct cagcatcgta
     8221 aagttttttt gctgcagaga ttacttcgct acgctcgtaa tgacgggaag aaaagagaat
     8281 tgcgctaatg gccaatgaca atctctaaag acctaagact gaaagaggtt gcttcgttcc
     8341 actgcatgac aaagtcaaca accaaataac tcaataacaa agtaaccaat aaccaaataa
     8401 cgcaataacc aaataaccaa taactatttc acaatgacca atgcccccga acttcgcgcc
     8461 tgtaaacaac aaacaaaaac aagcctaaca tcaattcgtt tacagccaat atcactccgt
     8521 tgcttgcaat attttgtgat tgtataaata cacttgcagc gtatatagct atagtcaata
     8581 agaggaaaac acccatcgtt ttgaggtcat aatttatagg ataatatttc agactcatga
     8641 tgtatgaaat tatcatcatg ctggcataag ttataaaagt agtaattgca gctcctgtgt
     8701 atcctatttg cggaatgaga ataatattta aaacaatagt tataacagca cccacaatag
     8761 aaatatatgc accaaactta gtttgcccac tcagcttgta ccacatactt tgattgaggt
     8821 aaatgccaag acataaattt gctgccagta aaataggtac aacatgcaat ccttcccaat
     8881 aatttttgcc aacaaaatat tttatccagc tcatattaag tatcacaaat aaaaagataa
     8941 acaggcacgt gatagaaaaa tatttcatca cccgggcaaa attttcttta ctgtttgaag
     9001 cattcgattg cgcaaaaaac aatggctcgg cagcataccg aaacgtttga ataaaaatgg
     9061 tcatcagtat cgaaatttta tacactgcac catatacgcc ttgctgatgc atagcgctcg
     9121 atgcaggttc tgccagatag gtgagtataa ttctgtcgag ggtttcgttt atcatgccgg
     9181 caaaaccagc caatagcagt ggcaacgaat accataacat ttgttttgtc aatgctttgt
     9241 caatgcccaa ttttacctgt gcaaactctt tatacaaaaa tagcaacaca actatactac
     9301 ctataatgtt ggcaataaaa actaccgaaa ctgtaagggg tggagaataa attaatgcac
     9361 taaaattgtg caacatacta ttgcttgatt gataccaacc tggaatcact ttgataaaaa
     9421 acaatgtaag cagtacggta gtgataattc cagtgagttt aatgtatgcg aattttttcg
     9481 cctttgcggt gctgcgcaaa tgtgcaaaag gaatcacact tagtgtatcg aaaaaaagaa
     9541 gggccgccat catgattata tagtgtgcgc caaattctat tttaaataaa tgttgaattg
     9601 ggttgcggaa aagtataatc gcactgctaa aaataacagt gctcaatagc atcatcgaaa
     9661 aagtattgtc gaatattttt ttctcgtttt gctgcttgcc aaaataaaaa tacgtggttt
     9721 ccatgccata agtgaaaagt atattggcaa aagaaataat tgcatatata taagaaatga
     9781 taccgtattc gggcggcaaa aaagtatacg taagcaaggg agtaagtaag taattgagaa
     9841 accgccccac catggtactt agtccgtacc aaacactttg cgaggcaagt ttttttatta
     9901 tactcattta ttgttaccta cgtatactgt atatgttaac gaaattattc gcctttaaat
     9961 gttgctttac gtttattcat aaatgcatct accccttctt tcatatcaga ggtggcaaaa
    10021 cattttccaa attgggcaat ctccatatcg aagccaccag aagtgtttgc agcattggca
    10081 caggctatac ttgccgatac tgccaaaggt gcttgctgtt ttattttttc ggcaatctct
    10141 ttgcacttgt tcatcaattc ggcagccggt accacgtggt tcacaagccc ccattgcaat
    10201 gcatcggtgg catttaaatt atcggcagtg atgagtaatt ctatcgcctt tggtttgcct
    10261 accaaatgca caagcctttg tgtaccacca tatcccggta taagaccgag ctttacttcg
    10321 ggctgcccaa actttgctgt ttcacttgct acacgaatgt gacatgccat ggcaagttcg
    10381 caacctcccc ccaatgcaaa actgttgatg gctgcaatta caggcttggc actattttca
    10441 aacatattga aaacggcatg accttgttgt gaaagagcac tgccttgctc tgctgataaa
    10501 gttgaaaatg cagaaatatc ggcaccggca acaaatgctt tttcgccagc accggtcaat
    10561 ataaccactg ccgtttctgc atcgctttgg caggctgtaa ttgcttgccc caattcgtca
    10621 attgtggcct gattaagtgc gttcaatttt tcaggacggt tcactgtgat agtggtaatg
    10681 cgattttcgt tttgcactac aatgtttgcg taactcataa ataaaagtat aatttgtttt
    10741 gattaaaaat ttctgtgttg ttttacccag ttggtaatgt atgcaactat ttcatcagtg
    10801 ttggtgccgg gctgaaaaag tttgccaatt cccaagtcgt ttaatttttt tatatcgcca
    10861 tcgggtatta ttccgccacc ggtaataaga atgtcggtag catttttttc tttaagtaaa
    10921 ttcaatattt ttggaaacac ggtcatgtgt gcacccgata aaatgctaac gcctataaca
    10981 tcggcatctt cttgtatggc agcgtttaca accatttcgg gcgtttggcg caatccggtg
    11041 tatattactt ccatgccggc atcgcgcaaa aacgaggcta tcactttggc tccgcggtcg
    11101 tgaccatcga gccccacctt ggctactaaa actcttattg gacgcatttt atttttttga
    11161 taccatttac ctgtttttaa atggggctcg aaaaatgaac aaaaaatccg tgtttaccaa
    11221 tatttgtaaa tgcatattcg aaacgccaca ctaaattata atacgaaacc acatcaatac
    11281 caactccata tcccgataga atattgttgt tgagtgtgtt gttttcgttg ttatatttac
    11341 ttcgtgcata acctgcatcg gcaaacatgt ttatataaat tgctatgggt attgtattaa
    11401 atttttcggt gcggataaaa ttcaatttta tttttcgcaa aggaagcaaa gcaaatttaa
    11461 aattattttt tgccacaaaa tgatattgcc cattcactac atacaattcg taaccacgca
    11521 caaaatcgtt accgtaaccc agcccacgtt gcaaataaaa tggcaccgtt ggcgaagaaa
    11581 atacttttgc ccgtacactt attgcatgat accatcgtgg cgaaagtttt ataaatcttc
    11641 ttgcacccaa agttgccgat aatattgccg gttcgttgtg caatagttcc aagccgtttt
    11701 gtgtaacctc accgcttata gcataaccaa tgagtggata tacgttgaag tctacttgct
    11761 cgtagcgata acttactccg gccaaaatca tttttattct gttatcgctc gattttaaat
    11821 agttgggatt aagtgtgctt aatgaatcgt ccaattttac atcgcggtac tcggcaaaaa
    11881 tattttgtgt gcggtatatt ccttttcgtt ttgtgtagcg caaccacgca tacgtttcgc
    11941 gcctcactac cgattgcgaa ttggtataaa attttaattg attgcctgcg agcgcataag
    12001 ctgtttcgcg atagcgctga gttcctgccg aaaatgccag cccttgcgtt tttgctttat
    12061 caatatatgg cacgttatac gttaagccaa gtttaagcga atatccaaaa cgcgcattga
    12121 tttgtaaatt ttctttgcgg ccgcgaaaat tttcgtgcaa caagtacatg ccataattaa
    12181 cacgcgaaaa atctttggtt tgccaccatt cgtttatgtt gcggtccact ccttcgaata
    12241 ttggaaatgg ccacacatac catttttctt ttacaatgat agtgatgtcg gcaccagtaa
    12301 tatcctgtgg cgttacatgt atctcaacaa aattgaacaa cgaagtattc atcacatttt
    12361 ccatactgcg ttgtgcagcg gcaaacaaaa catcggtgtt gagggtatcg ttcttgtgaa
    12421 aaatcagctc gcgaacaatg atggcttctt ttgttttttt atttccggta atggtaatgt
    12481 ttgcaacata aactgtagga tgcaagctat cgttcaggtg ccatactatt tgtgctaaac
    12541 ttacattact gcaaaagcaa ataataatgc tgcataatat cagatttcta aaagcaaaaa
    12601 atacactttt catttttgct aaaagttgct cattaatttt atggtgctcc cgttcattgt
    12661 ggtgcaagtt atcaataata gttttgagcg atttttatac caattccatt tttcgaaaat
    12721 gcattgttct caaatacgtg ttctgcgttt tgttctatca tccacaaaac gaaaattaca
    12781 caagcgtgtt tgcaaattcc ctggtcgaaa tatctgtttg cacataatca tcataagttg
    12841 gattggcaat aaaatttatt ttgttcatac atgcttctac aatatttggc atggcagtaa
    12901 attttatttt ctccttcaaa aaagcttcca cagcaatttc gtttgccgca ttcaatatgc
    12961 atggcatgtt gccgctttta tgcattgcat actctgcatg ggcgagattt ttaaatactt
    13021 ttttatcaac cgtttcaaat gtaagctgag gataattgat aaaattaaat cgtggaaaat
    13081 ctgtcttcaa tctgtttgga tatcctaaag caaaaaggat aggaagtttc atatcgggca
    13141 aacccatttg tgctttcata ctgccgtctt caaactgcac aatggaatga atgatagact
    13201 gaggatgcac aatcacatca atttgttcgg gcttgagatg aaacaaccat tgcgcctcta
    13261 tcatctccaa ccctttattc atcatgcttg ccgagtcgat ggttattttt gcacccattc
    13321 tccagttagg atgtttcaag gccatggcag gagtagcact ttctaaaaac gaaatatctt
    13381 ttcccctgaa aggcccgccc gatgctgtta aataaatctt ctctatttta ttatgccact
    13441 cacctataag gcattgaaaa atggcactgt gttcactatc aaccggataa atgtttactc
    13501 catgcttttg cgccaattca tttatcaatg cacccgcaac aacaagtgtt tctttgtttg
    13561 caagggcaat actttttccg gcttttattg catttatagt tggcaacaat cccgaataac
    13621 ccaccatggc agtaagcaca atatcgatat tgtccatttg caccacctcg gcaatagcat
    13681 tgttaccggc aaacactttt atatcatgcg gttccaaagc atcctttacc aacgcataat
    13741 ttgtagtatc agctataaca acagtattag gttttacttg taatgcctgt gcaatcagca
    13801 actcgtggtt actatttgca gtaagcactt caatatcgaa attatcactt tgcgccatac
    13861 acacttctat ggcttgtgtg ccaatgcttc cggtagaacc caatatggca atactttttt
    13921 tcaaatgatt ttaaatattt atgaagtggc gaaattattc attaggatgg aatataccag
    13981 aagtttgagg ttataagaaa aaaactattt atgcataggt gttattttaa gaaatggttt
    14041 ttgtggctaa aataattttt tgccgatttc tccatacaat aaatttttct tgctcaacac
    14101 attttccttt atacatttac agtgtcaaaa aatacaacat tgcttttcgg ataagaaaac
    14161 ccttatttac ttgttgtgtt taattatctt atttatacta aaatgtaacg catctatatt
    14221 caaacttaaa aaaaattaaa tttatgaaaa attctaattc aacaattcag caaaaaaaca
    14281 acccaaagga ttctaatttt gataagcaca agctatttgt aatccattta cttttaattt
    14341 tatttatagg aagcatggca aatgcacaaa gcattaagca aataggcccc ggtgccggag
    14401 gacaagaaag agcagtttat atttatccaa acccagttgg ttcttatgat ttgtatgtgg
    14461 gctccgatgt aagtggtgta tggcgcgcaa ctaatattcc tgcaaatgga tttgggaatg
    14521 tatacaatta cacttataat tttataagca atcacgaaat aatgcggttt gtaaataaat
    14581 tttatcgtgc cgatatgttg gctacaccct atttgttttg cctaaataga agtggcatcg
    14641 atagaattga tttgacaaac tacaacagca gtatgagtac agttcctgtt tttgaaaaca
    14701 gcgcagaaat tacaatggaa gactcttggg taagcgatat gtatgttaat gctacaagca
    14761 aacagactta ttttattaca ggcaatacga gagttactga tgatgggcct gggaatttta
    14821 agggaactaa tgtaagtgat atttttacag gaacacttac ttcaacttat aacagagtgg
    14881 acataaatgg caaaaaagca ttatttaatt taactacaaa taaccgagat gtttattgct
    14941 tgtatgtgga tgatcaatca aatactgatg caacggatga tataattttt gctggtgccg
    15001 aaaatggatt gcacataatt actcataatg aacttgctgg gacagcatcc ttactgccta
    15061 caacacaaaa ggcggggcct tctacaaacc aactcgaaaa taaagtaact agtttatttt
    15121 tggttagcaa aactacacaa ggaaacaata ctatgtatag ttttgtggca acggtgcatg
    15181 gcatgggcct ttatagatta gattgtgtcc gtaacactag caacttaatt tttacaacta
    15241 ttacttggac attattatca actaatgtta caggcataaa ccaaggaacg ccacaaggct
    15301 gtagtcaatc ggagccatat ttaattccaa aatttacaaa gtgccttcct gtttttaatg
    15361 ggcaaaatat agccggttat tatataacaa atgaaatcaa ccttgagaaa ttatgtacag
    15421 gaagacaagc caattatttt accggtgtat attattgccc ggccgatata gatggatttc
    15481 ctgatgcggg tggcttgaag ccattaactg tatggcaagg cggaaacgat tggggttgga
    15541 atgccaccaa gcctgcagca aatattaatg gtgccctaat tacaccagaa aatactttgc
    15601 tcattgggaa acaaggtaat atttttgtaa caagtaacga taacgccact cctgatgaaa
    15661 atgtacattg gcatcaaata tacgatcttc aaagtactgt cgctaattgc tataccgaaa
    15721 accagttcat tcaccgtggg tatataaaca ctgcaaataa aaccatttat cccagcacca
    15781 aaaccgatgt gtggttaggt caggccgatc gtcttatttt tcgcgataat aacagcaacg
    15841 attattatca cagtatagat gcaacaaata catgttacac atttaattat accggacaat
    15901 cgtgtggtaa caatttagga aaaaatgatt gttggtttat aaccaataat accattgatg
    15961 gcgaagacca aaacatttat attggaatag gcgaaggtta tgcatcgcaa gtagggtgcg
    16021 ggcatgtaat gtttggcgat aaagatggaa atacatttaa tgaaatagga gatccttcta
    16081 taaatggcga cccacgtaaa attttattta cctccgatgg caataaattt ttattagtaa
    16141 acaaaaaaat cagtgtcaac aaccacaaac tccgtttatt tactttggtt aataatatat
    16201 ggactgaaat tgatttatct acagtaaata attatgctgt gagcgatgca gttatctcgc
    16261 cagatggcaa aaatatattt gccataatta aagaagtaaa cgatgtgcat ttttatgcgt
    16321 ttccttttat caatggaata ttgggcacac cttgtattaa ttctgttggc acctctgctg
    16381 ttacaccagt aaggctatcc atgttacgtt atggagcgca tctggaaaat tacaaaattt
    16441 tgtgtggtac tgtaccaatt atagatggca ctactactga taattttttt gaagtatata
    16501 atagtgcact acccaatact tgtattccca attctattaa tccttcaaac ggtctatctc
    16561 ttatttttaa aaacttgcac aaagatgtgc tcgacaatac cgatgaagga atttctgcaa
    16621 tagctgtgaa cgaaaaaatg aatactttat ttgtagcaac agttactggc aatacatatg
    16681 attttgtttc gcctgtagca acaaaatctc atttgtacaa ggcatcgtac aattttctaa
    16741 caggcgttat tgacaataac tcgtggacag atataacctc cacgttacca aacaaaacaa
    16801 taattgatat tgccacacat ggcagctcca catgcgatga gtatttgtat ttatgtttgc
    16861 gaggccttgg cggttggcaa ttggcactac ctgctgcggt atctataaac aatgctttta
    16921 attcaatagc acccataaca ggttatggat tgctaactac aagtgttaca ttaggaccaa
    16981 atgcaaatat tgtaaccatt gatggtgcca atattggaat tggccctggt aaaaccatta
    17041 cagttccatc tggcaaaacg cttaaaataa ttaatggctc ccatttatat gcctgtggcg
    17101 atatgtggca aggaattgtt gttgatgctg ggggtacatt aattgttgat ggcagcataa
    17161 tagaagatgc agtattagca ataataaaca atggtttaaa actaacagtt atcaattctg
    17221 cattcgaaca caattatgtt tcaattacac taaatgatca aaattttagc acgaccgaga
    17281 tttatggcaa taaatttagc tgtaccagtg ggcaaatatc taaaatacca tacaatgggc
    17341 aacaaactcg ttatcacatt tctgttggtg gtgttgatgg ccttaccata ggtaatagtg
    17401 cattaaataa aaacattttt caaaatgcaa gtaaagccat ttttgttacc aatagcaatg
    17461 ttgatattat taataaccaa tttgaatgga taccaaacaa cctaaacact tggggcgagg
    17521 caatttacat tgatggaaaa ccaactataa gcaccatacg caaaatagga ggtaatgtaa
    17581 atgctaacga taaaaacact tttattaatt ggaagcaaaa tattaccgta agtaatgcgg
    17641 gcaaaatatt gattgaaaat aatgtaataa atcctgaccc tgcaaaccgt agttttgtag
    17701 gtataagcca acaaaaatgc actgatgtaa caataaaagg taatgctatt aaccgcagca
    17761 ataatggtat taagttgttt gataacccgg gtcctactgt aactgttact ctaaatactt
    17821 tcaacaaaaa cttcgcttat gattttacaa agtatgatga tgaagccata aggtttgaaa
    17881 ataatttaga catgaaaact aaactttaca ctataactaa taacgaaatt tataatgttg
    17941 ctgtaggctt gcatggaatg aatacgaatt caactttaga ttataatact tacaatacgc
    18001 atataacgct taacgactta acgacttatt acactacaca taatagtcag cctcattttg
    18061 gcatttggat gcaaaatgca tctggcagtg cgttacgtaa taatgttatt gagtggaatg
    18121 gcaatgcttt tattacttcc gatgagttga caaagttgca tgcaataaga atgcaaggca
    18181 gtggcagttg tgttttacaa gaaaatacaa tgacacgatt tggtgggggc attgatattt
    18241 ttgacgattg cagcaaaact caattagaat gcaacactat ggacggctgt taccatggcg
    18301 tttattttga cattaatggc ccaaacaaca aactcaccga ccaaggtgtg gctgtatttg
    18361 atcccttata caatgcagcg ctaagtaaaa cttggcacaa ccactggata ggtaatgtag
    18421 gaacaaataa agtggatgga cagtatttta atgctttctt caattggatg tattttgaca
    18481 atccttttga tgcggagtat gcaccaatac cagcgatacc attagttttt gctcgccctt
    18541 gttcactaaa tacatcaatt tgtgctgcac caattgtaaa tgacgaccat ttacgcatgg
    18601 ccgcctatgg agcagcagtg ggcgatagta gctatgccga ttatgattct accgaatatg
    18661 cccacagcga taaatcaact ttctataaag cagcaaaaat tgacagcgca gttttatact
    18721 taggtttgcc aagcgatgca atatatcaag cagagttcaa tagtttaaaa caaactgaca
    18781 ttggtttgtt cgaagatgct aaagatgcta tcaacaataa taacagttca cttgcttttc
    18841 agaaactttc ggccatggta aaacaaaaca ccattgatga gaataataat tacatgacca
    18901 tgttagttgc attaaattat aacgcaagtt tggatgctga ttccgatact atagttgtag
    18961 taaataacat tgctatgatg cacccatttt acggaggcga agcagtgtat attgcaagag
    19021 caatgttgca tttaaatatt gcagatgtat tgccgccatt aagaaggcaa cataaaaatg
    19081 tagaaagtct tgtaattgtt aatacaaatt ttagtttaat tccgaatcct gcaacaacgg
    19141 aggtatccat tttttctgcg caatcattta ttaatactaa gttgcaatta tatgattctt
    19201 atggctcatt ggtcagcaat atacccttac caaacaaaac aaattctttc acatttgata
    19261 taagcaattt ggttggcgga atttatttta cttacttaaa aagtgattta ggtatttcgc
    19321 aaatgaccaa gttagttatt attaaataaa aaactaaaac gtagagggaa tgtattgcat
    19381 tccctctttt ataattgttt atatgacgaa aaagtttgtt ttttttgttt cttatattat
    19441 tctctattta tgcaattact gtatttctca aaaacaaaat gtgatatggt gttttggcga
    19501 tagtgccgga attgatttca ataacttgac aattccaacg ccaattgcaa cagggggcga
    19561 tggcagaggt aactgcacat caatagcgga ttcaaatggt aatttgcttt tttatgcaac
    19621 tgaaaactca aactcggacg ctgttattta caacaagaat catactctta tggcaaatac
    19681 aagtggaatg ccttctgatg gctacttcgc aggcatggtt attattccaa agcctggttc
    19741 ggatagtttg tattatttgt tttatagagg aaaccctaat aatgttgata ctttttatag
    19801 agcgataatt gatatgagcc aaaacaatgg attgggagca gtaatagaaa aaaacaaagt
    19861 aatacaacca ataagttttg ccgattgcat tacggctgta aagcatggta atggaagaga
    19921 ttggtgggta attgacaaat attatgtaac accatttaca catcacaatc gtttttttgc
    19981 ttatctaata aaacctgata caatcatgcc tgccatcatt caagatttta atgatatgaa
    20041 tgatggtgct tttcaaaaaa tatgttggca cccttccttt aacaaattta tgcttataag
    20101 tattgtaggt tacatggctg agtttgactt tgatagatgc acaggtgcaa ttagtttgaa
    20161 cagaatcatt ttccctgagc aattaagtaa ttacaatcgt attttttggg aaggtgcata
    20221 ttcaccaaat ggcaatgtgt tttatgttag tcgcaatagt tatggtggca attttggcaa
    20281 ttataattac ttgcttcaat atgatttaac tgccaccaat atacctgcaa gttgcgatac
    20341 tttaaatttc acttcatatc cttcagcaga ttgcggtgct gtgcgccttg cacccgatgg
    20401 aaaaatttat tatacacatg catacatacc taccaatgca ctcaactacc cttatgcaga
    20461 taccatgcgc aactacataa acgaaaacct cggagtagta aataatcctg atgtggttgg
    20521 gccgggttgt aattttgcac ctttcagttt tcaccttgga ggtaaacgta c
//
LOCUS       DAORQA010000024        20115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_691_length_20115_cov_24.340429, whole genome shotgun sequence.
ACCESSION   DAORQA010000024 DAORQA010000000
VERSION     DAORQA010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 20115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20115
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_691_length_20115_cov_24.340429"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1639
                     /locus_tag="PK736_03275"
     CDS             230..1639
                     /locus_tag="PK736_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32446.1"
                     /translation="MAQKVKVAQGTSISTAAIKQGEAFNFMAGGDAYLIEKHFKDAEM
                     HFYLTCVTPNLGVNFNNYLNIPGGVFGNTYGIDDVFALGDKAYVTVEHLDKKAGMNSL
                     TIRSLGFDGNIATEETPAMNITFEKIMNSGFNHASVSLDKNLLAIVGETPFVKEQPAK
                     FKIAMFDKTLKKTSEAEITMPGENTKNKEMKVLVANDGTVYLIKKTTTNKGEIALTIY
                     QCNTSNVTDVKEYKIEMPAPNNVSSYTYAMNTNNEIIVGGTYYERRTLTVGDPKAMGI
                     FYFTNTGKSTPTFKMSTLDTPAENLKARKIIVNGNTVFLTTEQYKENRETPQPGAPAF
                     EYNYNYEHKNEYVIGMDAAGTKKFQLQLARDLTARNFDTHLYSGYYLCNGKLTVVYND
                     NTKKYIPNDTYFGMQLPVLVQITNDGLMQAPVVIKDESKLESGFMLFANSGMQVSENT
                     IIMLAGNAREMKPVSFVVE"
     gene            complement(1764..2990)
                     /locus_tag="PK736_03280"
     CDS             complement(1764..2990)
                     /locus_tag="PK736_03280"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010425088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC32447.1"
                     /translation="MKFVDFNFDYHLQDGLDSMGFEDATPIQSQAIPFILEGKDLIAC
                     AQTGTGKTAAFLLPILHKLSLEPSNVTNTLIIVPTRELAIQIDQALQGFSYFTGTSSM
                     AIYGGNDGTAFETERRALSQGANIIIATPGRLMAHLNLGYVKLQSLQHLILDEADRML
                     DMGFVDDIMKISTFLPKQRQTLMFSATMPPKIRTLAVKLLHHPEQINISLSKPAEGVT
                     QTAYVLYEKQKNELAKFLLKGKEDVYRSVLIFLSTKIKVKDLERDLIKGGLNVKAIHS
                     DLMQNEREKALNDFRSRNVQILVATDILSRGIDIEDISLVINYDVPGDAEDYIHRIGR
                     TARAASKGEAITFISEMDQGKFSRIENLIGYEVTKLPMPPHLGDAPAYDPLIRRPKTF
                     SGRNKNFRKPQSRRVR"
     gene            3207..4331
                     /gene="mqnC"
                     /locus_tag="PK736_03285"
     CDS             3207..4331
                     /gene="mqnC"
                     /locus_tag="PK736_03285"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HRC32448.1"
                     /translation="MKTDELLKRALNFEFLSIEEGVYLFEHAPTSALSFVANELRKIQ
                     VPGNKVTWIIDRNVNTTNVCIANCKFCNFFRIPGHAESYITDIETYKRKIDETFRYGG
                     DQLLLQGGHHPDLGLSFYTNIFRELKSLYPTLKLHSLGPPEIAHISKLENKTHTEVLT
                     ALKEAGLDSLPGAGAEILNDRVRRMISKGKCTGREWLNVMRAAHHLNITTSATMMFGH
                     IETIYERFEHLVWLREVQAEKPAHAKGFIAFIPWPFQDEDTILRNVKGIRNHVTADEY
                     VRMIALCRIMLPNIKNIQASWLTVGKDVAELCLHSGANDFGSIMIEENVVSAAGAPHR
                     FTSQGIQDAITEAGFTPQLRTQQYEYRDLPKVMEQQVIDY"
     gene            complement(4424..5101)
                     /locus_tag="PK736_03290"
     CDS             complement(4424..5101)
                     /locus_tag="PK736_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32449.1"
                     /translation="MHIQKSIRIHARPYGAGSYKSVSSYFDYKFSFKPKKANANFTAS
                     LSYRNNVLRYYNWTSAVNENKICHLNVNRPLAGFSLEIGNEFRIKNKSHFLYGCGIGA
                     LYSLNNILNKTTTGITYDYRYPIMDKDIDSSHYLISDIQTIELVPTLVAYLGYNYALK
                     SNLILCFGVRFETSFFEGQTQARTYVYYDDYKAYSYTTDYITTNNSSFIFSTGIKFSN
                     NIFKKRG"
     gene            5391..5651
                     /locus_tag="PK736_03295"
     CDS             5391..5651
                     /locus_tag="PK736_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011242652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRC32450.1"
                     /translation="MPEISRFLGIIIRMFYNEHNPPHFHAYYNEFQVEIRIDTLEVLK
                     GSLPKRVLNLVIEWAIDNRAELLNDWELMRNDKAPLPIKPLI"
     gene            5651..5881
                     /locus_tag="PK736_03300"
     CDS             5651..5881
                     /locus_tag="PK736_03300"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRC32451.1"
                     /translation="MSTIPNIINVLAKDNYLLEIYFDDGKYKEIDVKQFIKNGVSSRL
                     LDIEYFKRVKVQEGFICWDNGYDFCPEFLYNL"
     gene            complement(6114..6425)
                     /locus_tag="PK736_03305"
     CDS             complement(6114..6425)
                     /locus_tag="PK736_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32452.1"
                     /translation="MNHVDTVYQGFKTEYIKCDALGNANYTLTNFAMPKYFTGLQSIP
                     GFELDYQLFILNEETYNFIISQNENSISGFSFGDGTPVQLYNNFKTGEGFFGYMLEIK
                     K"
     gene            complement(6494..7009)
                     /locus_tag="PK736_03310"
     CDS             complement(6494..7009)
                     /locus_tag="PK736_03310"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC32453.1"
                     /translation="MNKFLILFFEIIFVLAPKFNLAQKEIIITGVVTDGNSDQSIREA
                     KIVVVKPYQELIADNNGYFKFKILQPDTIKFFVSKEGYKYQEFNLPITTDTFFKISMT
                     FYSLPQIEVEGESLNFISKSDCKYYISMNLSLAGYSSAEPDSVSVHVCNFQSQKLYLQ
                     TAKFSSHIHFH"
     gene            complement(7142..7690)
                     /locus_tag="PK736_03315"
     CDS             complement(7142..7690)
                     /locus_tag="PK736_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32454.1"
                     /translation="MDFSYRNNKFKYDYDNPTRYADVVHFFNYGRPQLGISLMFVNKF
                     KFTNKIFLQLGCGLGMLVSTNEIIRNANDGEKVATIYPKNNIDSVYYYLSTIHQNTIC
                     PTVTCALDINYYLNANIVLCMGIKCETGFIDSQSQLLLYNSYIFYGNYSTILEFASTS
                     NTSIIFTTGIKFSNNIFKKTKK"
     gene            complement(7964..9124)
                     /locus_tag="PK736_03320"
     CDS             complement(7964..9124)
                     /locus_tag="PK736_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32455.1"
                     /translation="MKKLNYLWITLLLVFYGSCKKQNEAIESKTITSTNSNAKIDDEA
                     IYDAMLRTAAIGLLYYQNDVAFRATVNAGVAKQFDGDDNVLMRELGAAYANMPTNFQT
                     SINLHGTGLNCYTDLPHIGEAINGFEYFDDSAYIQIYIPNADIVDLNSTPIICINTDD
                     DEVLPGYYIDASGVLQVIDEVDEEYAETHLVWVVSVNETTGIKFGGEEINFGSYFPAP
                     PTADDMLFRNDYIYFWDKKENWGNGRGEFSYQVTKYLSCSDDPNHHVEFTGINAFRIC
                     KNDLNKNFVWEKYFANYEPGFNDVWRGNEFLGIIFYELDKKRTFDIKINFDSPCYGYI
                     ITSKQDPYYTARLERTFGSSSSWVTFVLPTQLLGPENRPSNGADFHASSKNY"
     gene            9292..9690
                     /locus_tag="PK736_03325"
     CDS             9292..9690
                     /locus_tag="PK736_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC32456.1"
                     /translation="MNYIGYKIKKIRELKGLSQGYMAKQMNIEQPQYSRIEQSDDKIS
                     AKYLEAIAAIFEMKMDDIIHFDEKVIFNISNHNNHLEKGVVYQNGMEHEILLKQIQDK
                     DKKNQDLMLENYKLKAKLKKKSALPLRNSK"
     gene            9698..10948
                     /locus_tag="PK736_03330"
     CDS             9698..10948
                     /locus_tag="PK736_03330"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRC32457.1"
                     /translation="MEATIKNIDTKLKTPAQSRADFPILQEVIYKNKSLVYLDNAATS
                     QKPQVVIDSIKNYYEHYNANIHRGVHYLSQRASAAYDAVRGKVATFINAASEKEIVFV
                     RGTTEAINLVASTWGRKNILPGDEIIISTMEHHSNIVPWQMLCEENNAVLKIIPMNDA
                     GELMMDEYEKLLTPKTKLVSVVHTSNSLGTINPVEKIIALAHKCGAVVMIDGAQAIVH
                     DVIDVQKMDCDFFAFSAHKMCGPTGVGVLYGKQNILEQMPPYQGGGDMIKSVTFAKTT
                     YNDIPFRFEAGTQNIADVIALGVAVDYYNSLDRIQCLVHETALMEYTMQQLKQIEKIK
                     LIGTATNKASVVSFVIDGVNAMDAGMYLDTMGIAVRTGQHCTQPVMDQLCIAGTVRAS
                     YMFYNTMEEVDKLVEGVKKVVSAF"
     gene            11117..11779
                     /locus_tag="PK736_03335"
     CDS             11117..11779
                     /locus_tag="PK736_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRC32458.1"
                     /translation="MNLQQLINKELPPLKTTDTVAKALNWMQVFKVRQLPVLKEKTFI
                     GVIEEEHLKKISDVNLPLAATDVKIVRSFMYNDQHYYGAFTFCTNNQLEIIPVLNREG
                     HYLGLITAASLIDCFAQIKWASDDCSIIVLECPMRDYLLSKIINIAESNGANVLSVDV
                     NLMADGNTNEVTMRLNNIDLARIEAGFFRHDYKITASYSTALVNPDFQDRYDSLMKYL
                     GI"
     gene            11908..14631
                     /locus_tag="PK736_03340"
     CDS             11908..14631
                     /locus_tag="PK736_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC32459.1"
                     /translation="MKTIQTPALKSLILLAFIATIIFTGSCSRKRNTMVSRAWHNTTS
                     HYNGYFNAREKVQEVAKKLATAQPDKFERTLSIFKLGDDNQAKANYPDLDEAIKKSSI
                     VIQKHSIKIKGKEYCKWIDENYLMVGLAQFYKHDYWTAIEAFQFTASEYKESNSRYDA
                     MIWLTLTYMQLGKMVDAEYVIDFLNNQSKMTPKQKQLFSLVKADYYSKKDDIPNEIKA
                     LEEAVANSRKKDDKVRYRFILAQLYQKLGDSRKAYGLYEKVIKMNPYYELAFNAKINR
                     ARVFDAESASGGTVKKQLNKMLRDDKNKEYRDQIYYALAGIAKKESKELEEIDLLKKS
                     VATSVSNNNQKAISYLDLGEISFSKKNYRAAQAYYDSTMQFLTKDYPTYDQISNKKST
                     LTRLVENMMVIENEDSLQAIAKLSEKEREDYIAGIIKKENEEKERQKLEAEKKKQEEI
                     QNENQVNQTLNEENIRKNTNQQIPGAGNGAWYFYNTATLSFGFNEFIKKWGNRPLEDN
                     WRRKEKESQALANSEQGEDKIDMTAYNDSISKLADAKRKEIYLKKLPVTSEALVASNA
                     KIDEAYYNIGIIYREQLKEYPQSRDNFEGLLERFPDSKFKLPSYYNLYRLYSALGNAE
                     KAEYYKNLLLTKYADSDYAKLILNPEYFNNKQQRADIIQTYYENTYKAYQNQQFLAVI
                     ERKNEADSLFPPNSLTPKFELLKSLAIGKTRPISDFELSLRDVIAHYPKDSVSITAKN
                     ILDYITKNNLNGSKKAVNQEVTDSSKTENINQSDSTQANNELPKTPFAFNPDTIHYFV
                     MVFENKTINLNELKIKISNFNTKFYSVAGYQINNAFIGSEKQYLAVKQFTNREEASSY
                     METLLSDEEVFDDKSNYEYQVFLISPSNFGKLMVTKDIEGYLNFYDEQYSQ"
     gene            14938..15366
                     /locus_tag="PK736_03345"
     CDS             14938..15366
                     /locus_tag="PK736_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRC32460.1"
                     /translation="MFSNKPKTNNTSSEATSSSINLIGAGTVIDGEIKSNGDIRIDGT
                     VNGAVHSKAKVVVGPTGVVEGDVHCQNADISGAVKGTTHVSDTCFLKATSRIVGDIVT
                     GKFVVEVGANFTGNCNMGQVKEINYADRQKEQPAIRQKTG"
     gene            15317..15550
                     /locus_tag="PK736_03350"
     CDS             15317..15550
                     /locus_tag="PK736_03350"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRC32461.1"
                     /translation="MPTDKKSNQPSDKKPVDNYIRFSTMGLQMGATIFLFTYAGIKLD
                     EWLKLSFPAFTISLALFSVIASIYVVAKQAMKG"
     gene            complement(15555..16433)
                     /locus_tag="PK736_03355"
     CDS             complement(15555..16433)
                     /locus_tag="PK736_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRC32462.1"
                     /translation="MWQHYSDIIAQSYSGYYNYLLKEILYPGWHNYFYWLLGLSLLVW
                     LLEIVFPWRTAQAVVRKDFWLDGFYMFFNFFLFSLIGYNAISNIAVDVFNNFLSRIGI
                     ENLVAINIKSWPVFLQFLLMFVIADFIQWNIHRLLHRVPRLWEFHKVHHSVEEMGFAA
                     HLRFHWMETIVYKTLQYIPLAMIGFGISDFFIIHIIAVALGHLNHSNIPLNYGPLKYV
                     FNNPAMHIWHHAKELPAGKYGVNYGLSLSVWDYIFGTAYIPHTGRDIKLGFENVEQYP
                     HSFFKQILEPFKRTKL"
     gene            complement(16439..19390)
                     /locus_tag="PK736_03360"
     CDS             complement(16439..19390)
                     /locus_tag="PK736_03360"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRC32463.1"
                     /translation="MVKNIFYTALIGATLLFASTSKAQKKKGVSDFPLPSTSQTREDA
                     FEQRKKLNSTSLLNNVKLKNVGPTLMAGRVVDIDVSPYDATQFYVAFASGGLWKTTNN
                     GNTFTPLFDNEAVMTIGDIAVDWQHGEILYVGTGENNSSRSSYSGVGVYKSRDKGKTW
                     QHIGLPNTQHIGRIIIHPTQPNTIWVAALGHLYTANDERGIFKSTDGGATWKHTLFVN
                     DNTGAIDLIIDAKNENILYTSMWHRERRAWNFTESGSSSGIYKSIDGGNIWKLVSGAQ
                     SGFPQGEGIGRIGLAISATHSNIVYAVLDNQTRRPKKPEEALSQNKILSRDTLRQISK
                     ETFLQLSDKQIDDYLDENNFPEKYNAASVKKMVRENKITPSALVEYLEDANSLLFDTD
                     VTGGEVYRSDDAGTTWKKQNEKYIEELFNTYGYYFGQIKISPFNDDEIYLPGFQILKS
                     TNGGKTFSSILADNVHVDFHSIYINPNREGHIILANDGGLNVSYDSGATYVKCNTPPV
                     GQFYSINFDYQTPFNLYGGLQDNGVWQWQQNKADYGYADNNGQAYKALMGGDGMQIQI
                     DTRDDNIAYTGYQFGNYYRINIATGDQKYITPQVELGERPLRWNWQTPILISKHQGDI
                     IYMGANKLYRSMNKGDTWKAISNDLTLGGIKGDVPYGTITCIDESSLRFGLLYTGSDD
                     GLIHVSKDGGNTWKKISDGLPQKYWVSRVAASSHVEGRVYVSLNGYRFDNFSAFIFVS
                     NDYGNTWQPIGKDLPAEPVNVIKEDPANENIIYAGTDHGLYVSLNGGKTFMQWKNEMP
                     AVPIHDLAIHPRDKKILIGTHGRSAFIGDVSLIQQLYDTLINKPFYVFDIENKKHSSR
                     WGQKQGEYEPVIKPSQSFAYYAGKSGIVKLQIKADDLLLSTVTDTCEQGINFIDYHFT
                     FDSTNVAAYTLMVNKNKTGDEKIILKKADDGNYYLQPGKFTLVFTGPKNENIIKEFEI
                     KARERKSRSQQ"
     gene            19694..20053
                     /locus_tag="PK736_03365"
     CDS             19694..20053
                     /locus_tag="PK736_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32464.1"
                     /translation="MNSRLKIFLAFLAIGFLGSCTNDKADEIVPNLNCDTTYYQTTIK
                     PIVIANCAIQGCHVTGGQSPNFISYANIFDNRNKIKTNINLPSNDNNIMPPSGPLSAA
                     DLTKINNWIDSGAAGCD"
BASE COUNT         6706 a         3621 c         3623 g         6165 t
ORIGIN      
        1 cgttactctt ggctttcatt attagttaat agttataatc tctgttatga aatattaaat
       61 agacttattg gattctgcaa aaccaaataa ataaaccata catttagatc aaaacacatt
      121 ttagaaaaaa tataattaaa cataaacgaa cattaaaaaa taaataaaaa tgaaattttc
      181 aaaaactatt ttaatcattt tactatgctg ttttgtcatt aatttatcta tggcacaaaa
      241 agtaaaagtt gcacagggaa catccatatc tacagcagca ataaagcaag gcgaggcatt
      301 taattttatg gctggtggcg atgcatacct tatagaaaag cattttaaag atgccgaaat
      361 gcatttttat cttacttgtg ttactccaaa tctgggtgta aatttcaata attacttaaa
      421 tattcccggt ggtgtttttg gcaatactta tggtattgat gatgtatttg cacttggcga
      481 taaagcatat gtaactgttg aacatctcga taaaaaagcc ggtatgaatt cactcaccat
      541 tcgcagttta ggtttcgatg gaaatatagc caccgaagaa actccggcca tgaatatcac
      601 cttcgaaaaa attatgaatt ccggtttcaa tcatgcatcc gtatcgctcg ataaaaactt
      661 gttggccata gttggcgaaa caccatttgt aaaagaacaa ccggcaaagt ttaagattgc
      721 tatgtttgat aaaacactaa agaaaacaag tgaagccgaa ataactatgc ctggggaaaa
      781 tactaaaaat aaagaaatga aagtgcttgt ggcaaatgat ggtactgtgt atctcataaa
      841 aaaaacaact acaaacaaag gcgaaatagc gctaactatt tatcaatgca acacaagcaa
      901 tgtaaccgat gtgaaagaat ataaaataga aatgccggca ccaaataatg tttcgagcta
      961 cacttatgcc atgaatacca ataacgaaat aattgtaggc ggaacctatt atgaacgcag
     1021 aacccttact gtaggcgacc caaaagcgat gggcattttt tactttacca acactggtaa
     1081 atcaacacca acttttaaaa tgtcgacact cgatacgcct gccgaaaatc ttaaagcccg
     1141 aaaaataatt gtgaatggca atactgtttt tcttaccaca gaacagtaca aagaaaatcg
     1201 tgaaaccccg caacccggag caccggcatt tgagtacaat tataactacg agcacaaaaa
     1261 cgaatatgtt attggaatgg acgcagcagg cacaaaaaaa tttcaattgc aacttgcccg
     1321 cgatttaaca gcgcgtaatt tcgatacaca tctttattcg ggttattatt tgtgtaatgg
     1381 aaaacttact gtagtgtata acgacaatac aaaaaaatac atccccaacg atacatattt
     1441 tggaatgcaa ttgccggtat tagtacaaat taccaatgat ggtttgatgc aggcacctgt
     1501 tgtaataaaa gacgagagca aacttgaatc gggctttatg ctgttcgcaa attcaggcat
     1561 gcaggtttcc gaaaacacta tcatcatgct tgccggcaat gcccgcgaaa tgaaacctgt
     1621 aagctttgtt gtagaataat ttatatcaaa ggtttgtttc cctttggtgg cgtagccaag
     1681 gtttgcgtcc tcatggcgta gccaagttgt gcgtgtagcc aaggtttgtg tccccacaaa
     1741 ccttctctct gcacaaccaa caactaccgc actctcctcg attgtggctt gcggaaattt
     1801 ttattgcggc cgctaaatgt ttttggtctt cttattaatg ggtcataagc aggggcatcg
     1861 ccaaggtgtg gtggcatggg caattttgta acctcgtagc ctataagatt ttcgatgcgc
     1921 gaaaatttac cctggtccat ttcgctgata aaagttatgg cctcaccttt cgaagccgcg
     1981 cgggctgtgc gtcctatgcg gtgtatataa tcttcggcat cgcccggcac atcataattt
     2041 attaccagcg atatatcttc aatatcaatt ccacgcgaaa gaatatcggt agccaccaat
     2101 atttgcacat tgcgactacg aaaatcattc aacgcttttt cacgttcgtt ttgcatcaaa
     2161 tccgagtgta tagccttaac attcaatcca ccttttatca aatcacgctc aaggtctttc
     2221 acttttattt ttgtcgatag aaaaatcaat acacttctat acacatcttc tttacctttc
     2281 aataaaaatt ttgccagttc gtttttttgc ttttcgtaca acacataagc agtttgtgta
     2341 accccttcgg caggcttcga tagcgaaata tttatttgct cgggatggtg caataatttt
     2401 acagcaagcg tgcgaatctt aggtggcata gttgccgaaa acataagcgt ttgccgctgc
     2461 tttggaagga aagtagatat tttcataata tcgtcaacaa aacccatatc gagcatgcgg
     2521 tcggcttcgt caagtattaa gtgttgtaga ctttgcaact ttacataacc caaatttaaa
     2581 tgtgccatca agcggcccgg agtggcaata ataatatttg caccctggct tagcgcccgc
     2641 ctttctgttt cgaatgctgt gccatcgtta ccaccatata tggccatgct gcttgtaccc
     2701 gtaaaatacg aaaacccctg caaggcctgg tctatttgta ttgcaagttc gcgcgtgggc
     2761 actatgataa gtgtattggt aacattgctt ggttcgagcg aaagtttatg caatatgggc
     2821 aacaagaatg ccgcagtttt tcctgttcct gtttgtgcac atgctattaa atctttacct
     2881 tcaagtataa atggtatggc ctgcgattgt attggtgtgg catcttcaaa tcccatggaa
     2941 tctaatccat cctgcaagtg ataatcaaaa ttaaaatcta cgaatttcaa actattattt
     3001 ttttacgaag atagaaaatg ttttgtaacg attgatttcg ataagatttc aagccatttc
     3061 acatcattat ttttttgaaa tatttcatgc ctcaaactta aacatgcaac aattgttaac
     3121 tgtattaatc taggaagaat gcttcataaa tctgaaaaaa aatatggatg tctgtatagt
     3181 aaaattgttt cccttacatt tgccaaatga aaactgacga attactcaag cgcgcattga
     3241 atttcgaatt tctttcgatt gaagaaggcg tatatctttt tgaacatgca cctacaagtg
     3301 cactgagttt tgtagcaaat gaattgcgca aaattcaagt gcccggcaac aaggttacat
     3361 ggataataga ccgcaatgta aacaccacca atgtatgtat tgccaactgc aagttttgca
     3421 atttctttcg catacccggc catgccgaat catacattac cgatatagag acatacaaac
     3481 gaaagataga tgaaacgttt cgttacggtg gcgatcaatt attattgcag ggaggtcatc
     3541 atcccgattt aggtttgagt ttttatacaa atatttttcg cgagttaaaa agtttgtacc
     3601 caactttaaa attgcattca ctgggccctc ccgaaatagc acacataagt aaactcgaaa
     3661 ataaaaccca caccgaagta ctcaccgcat tgaaagaagc cggcctcgac tcgttgcccg
     3721 gtgccggtgc cgaaatactg aacgaccgtg tacgcagaat gataagcaaa ggaaaatgca
     3781 ccggccgcga gtggctcaat gtaatgcggg cggcacatca tttaaatatt accacatcgg
     3841 ccaccatgat gtttggccat atagaaacaa tatacgagcg cttcgaacat ttggtatggc
     3901 tgcgcgaagt gcaagccgaa aaacctgcac atgccaaagg tttcatcgct tttattccat
     3961 ggccgtttca agatgaggac accatactgc gcaatgtaaa aggcatacgc aaccatgtaa
     4021 ctgccgatga atatgtgcgc atgattgcct tgtgcagaat aatgttgcct aacattaaaa
     4081 atattcaggc atcgtggctt acggttggta aggatgttgc cgagttgtgc ttgcattcag
     4141 gtgcaaatga ttttggcagc attatgatag aagaaaatgt agtgagtgct gcaggcgcac
     4201 cacacaggtt tacatcgcaa ggcatacaag atgccataac cgaagcaggt tttacaccac
     4261 aactacgtac acaacaatac gaataccgcg acttgccaaa agtaatggaa cagcaggtga
     4321 ttgattattg attttcttga tttctattcg gacgaaaaaa ttaatggtat gtacttgtaa
     4381 gaattgaaaa gtgaactaat gtaattgtat acgatgtttt ttattagccc cttttcttaa
     4441 aaatattatt cgaaaatttt atgcctgttg aaaaaataaa cgaggagttg ttggtggtga
     4501 tataatcagt tgtatacgaa taggctttat aatcatcgta atatacataa gttcttgctt
     4561 gtgtttgacc ttcaaaaaaa cttgtttcga accgtacccc aaaacacaaa atcaaattgc
     4621 tttttaaagc atagttataa cccaagtatg ccaccaatgt aggaacaagt tctatcgttt
     4681 gtatgtcgga aataaggtaa tgagatgagt ctatatcctt atccatgatt gggtacctat
     4741 aatcataagt gataccggtg gttgtcttat tcaaaatatt attcaaagaa tataatgcac
     4801 caataccaca accgtataga aaatgtgatt tattttttat tcgaaattca ttacctattt
     4861 ctaatgaaaa acctgcaagc ggcctgttca cattcaaatg acaaatctta ttttcgttta
     4921 cagccgatgt ccagttataa tatcttaaaa cattatttct ataggataac gaagcggtga
     4981 aatttgcatt tgctttttta ggtttgaaac taaatttgta atcaaaatat gaacttacac
     5041 ttttgtagga gcccgcgcca taaggcctgg catggattct aatagatttt tgaatatgca
     5101 tatttattcc agcatttatg gaatgcgaaa aggattggcc gttacaaatt ttactgatta
     5161 gaaaaaaaga aatcaataca attgaagtaa atatttttgc atcattgcgg tagagcggtt
     5221 ttgttaaatt gaaatttttg gagttgattt aagaacaata atttgagatt attattgtgc
     5281 agcgaaacta gttttgagcg tataaatgct tattcaaaac caaatattgg taagtcaatc
     5341 attattttta tatttcaatt atatagctag aaaattcata cttttgtgtc atgcctgaga
     5401 ttagtagatt tttaggaata attattagga tgttttataa tgaacacaat cctcctcact
     5461 tccatgctta ttacaatgag tttcaggtcg aaattcgaat agatactttg gaagtattaa
     5521 aaggcagttt gccaaaaaga gtgttgaatc ttgttataga atgggcaata gataatcgtg
     5581 cagagttgct aaacgattgg gaattaatga gaaatgataa agcgccttta ccaattaagc
     5641 cactaatcta atgagtacaa ttcccaacat tataaatgta ttggctaaag ataattactt
     5701 gctcgaaatt tattttgatg atggtaaata caaagaaata gacgtaaagc aatttattaa
     5761 aaatggcgtc tcttcccgat tgcttgatat agaatatttc aaaagagtta aagtgcaaga
     5821 gggttttatt tgctgggata atggttatga tttttgcccg gagtttttat ataatttgtg
     5881 agttcgcaat tcaattgacc tttattttaa tattgaatac agttatagtt gatggccggt
     5941 aaaaattatg accatgcaat ccaaaaagaa acaaatgttt tttgcatcat tacggtgaat
     6001 tggttttgtt aatttatcat tgctaggatt agatatacga aaagcatatt gaaactatta
     6061 ttgcggaagc gaaaaatact gaagattgaa aaaaagcgca ataaaatcaa tttctacttt
     6121 tttatttcaa gcatataccc aaaaaagcct tctccggttt tgaaattatt atacagttgc
     6181 acaggagtgc catcaccgaa actgaaacct gaaatgctgt tttcattttg tgaaatgata
     6241 aaattgtatg tttcctcatt aagaataaac aattgataat ccaattcaaa accaggtatt
     6301 gattgtaggc ctgtaaagta ttttggcatg gcaaaatttg taagtgtgta attcgcatta
     6361 cccaaagcat cacattttat atattctgtt ttgaagccct gataaactgt atctacatga
     6421 ttcacaggat ggatatgtgg ttttgttatt actaacttgt agtatttatt ccgatttgct
     6481 ttatcttcaa actttagtga aagtgaatgt gactggaaaa tttagcagtt tgtaaatata
     6541 gtttttgaga ttgaaaattg caaacatgaa ctgaaacgga atcaggctct gctgaggagt
     6601 aacctgccag ggataagttc atgctgatgt aatatttgca atcacttttc gaaataaaat
     6661 ttagcgactc accttcaact tcaatttgtg gcaagctata gaatgtcata ctaattttaa
     6721 aaaaagtatc ggtagtgatg ggtaaattaa attcttgata cttataacct tctttcgaaa
     6781 caaaaaattt aattgtatcg ggttgtaaaa ttttaaactt aaaatatccg ttattatctg
     6841 caattaattc ttggtaaggc ttcacaacaa caatctttgc ttcgcgaatt gactggtctg
     6901 aattgccatc ggttacaacc cctgtaatta ttatttcttt ttgtgctaaa ttaaattttg
     6961 gtgccaacac aaatattatt tcgaaaaaaa gaattagaaa tttattcaca ctgaatctag
     7021 ttgaaaaatt tagtagtttg taaatatagt ttttagagtg aaaattgtaa acatgaactg
     7081 aaacggaatc aggctctgcc gaggagtaac ctgcaaaaaa tatgtaaata gcttaactcc
     7141 tctacttttt tgttttctta aaaatattgt tcgaaaattt tatacctgtt gtaaaaataa
     7201 tggaggtatt gctagttgaa gcgaactcca aaattgttga gtaattgccg taaaatatat
     7261 aagaattata aagcaacaat tgcgattgag agtctataaa acctgtttca cattttattc
     7321 ccatacaaag tacaatgttt gcattaaggt aataatttat atcaagagca catgtaactg
     7381 tcggacaaat ggtgttttga tgtattgtgc ttaagtaata atatactgaa tcaatgttgt
     7441 ttttaggata aatagttgcc accttttcgc catcattggc atttcgtatt atctcattag
     7501 ttgaaactag cataccaaga ccacaaccca attgtaagaa tattttgtta gtaaatttaa
     7561 acttattgac gaacatcaag gaaattccaa gttgaggacg accataatta aaaaaatgta
     7621 ctacatcggc atatcgggtt gggttatcat aatcgtattt aaacttattg ttacgataac
     7681 tgaaatccat tttgaaaaaa gtgttatatt ttgaattatg gtattcaagc ccataattaa
     7741 taaaggtact tttagtatat ttattggttg ggcgactttg gccatagttt aatcttttgt
     7801 atatgtgatt atttaaacca aaatcaaaat tgtgtataaa attttgacta tgacaaattg
     7861 agcaaattaa agcgaaaaca attgtgatgc taattttttt atttttcatt taaataattt
     7921 aaaagagtga gtttaaagca aactcactct tattcaaatc gaattaatag ttttttgaac
     7981 ttgcatggaa atccgctcca ttagacggtc tgttttcggg gcctagcagt tgtgtaggca
     8041 aaacaaacgt cacccaactc gaagaactac cgaacgttct ttcgagcctt gcagtataat
     8101 acggatcttg tttggaggtt atgatatatc cataacatgg ggaatcaaag ttgattttaa
     8161 tatcaaatgt acgcttctta tccaattcat agaaaattat acccaaaaat tcgttacccc
     8221 tccaaacatc attgaagccc ggctcataat tggcaaaata tttttcccaa acaaaatttt
     8281 tatttaaatc atttttgcaa attctgaatg cattaattcc agtaaattct acatggtgat
     8341 ttggatcatc tgaacaactt aaatatttag ttacttgata agaaaactct ccacgaccat
     8401 ttccccaatt ttccttttta tcccaaaaat aaatataatc attacgaaac aacatatcgt
     8461 cagctgtagg tggagctgga aaataacttc caaaatttat ttcttcgcca ccaaatttga
     8521 tccccgtggt ttcattaaca gaaaccaccc aaactaaatg tgtttcggca tattcttcgt
     8581 ctacttcatc tattacttgt aatactccac tcgcatcaat gtaataacca ggtaaaacct
     8641 catcgtcatc tgtgttgata catattatag gagtagaatt taaatcaaca atatctgcat
     8701 taggaatata aatttgtatg tatgctgaat catcaaaata ttcgaagcca ttaattgctt
     8761 cacctatgtg aggtaaatct gtataacaat tcaaaccagt cccatgcaaa ttgattgatg
     8821 tttggaagtt tgtaggcata tttgcataag ccgctcctaa ttctctcatt agtacattat
     8881 cgtcaccatc aaattgcttt gcgactccgg cattaactgt cgccctaaaa gcaacatcgt
     8941 tttgataata caataagcca attgccgctg ttcttaacat tgcatcataa attgcttcat
     9001 catcaatttt tgcattagaa tttgttgatg tgattgtttt actttcaata gcttcgtttt
     9061 gttttttaca agacccataa aaaaccaaaa gcaaagtaat ccataaataa ttgagttttt
     9121 tcattttcgt ataatttttt tttgtatgcc tactctataa agttttcagc tacccttttt
     9181 tgttttatca aattagcaac aaaggttttg cattaaaaat atcggtttgg ttataactta
     9241 tatcaaatct gatataattt atgttagttt tgtgcgaaac aaaaaattat tatgaattac
     9301 ataggttaca aaataaaaaa gatacgcgaa ttgaaagggc taagtcaagg ctatatggct
     9361 aagcaaatga atatagagca gccgcaatac agccgcatag aacaatcgga cgataaaata
     9421 agtgcaaaat atctagaagc tatagcagct atattcgaaa tgaaaatgga cgacataatt
     9481 cacttcgatg aaaaagtaat ttttaatatc agcaaccata acaatcatct tgaaaaaggt
     9541 gttgtatatc aaaatggaat ggaacacgaa attttattga agcaaataca agataaggac
     9601 aaaaaaaatc aagatttaat gcttgaaaat tacaaactaa aagcgaagtt gaaaaagaaa
     9661 agcgcattgc ccttgcgtaa ttcaaaataa aaaatcattg gaagcaacaa taaaaaatat
     9721 cgacacaaaa ttaaaaacac cggcacagtc gcgtgccgat tttcctatac tgcaagaagt
     9781 gatttataaa aataagtcgt tggtttattt agataatgca gctacatcgc aaaagccaca
     9841 agtggttatc gatagcataa aaaattacta cgagcattac aatgctaaca tacaccgtgg
     9901 tgtacattat ttgagtcaac gggcatctgc agcttatgat gctgtgcgtg gtaaggtagc
     9961 cacttttatt aatgcggcaa gcgaaaaaga aattgtgttt gtacgtggca ctaccgaagc
    10021 tataaatctt gtagcatcta cctggggaag aaaaaatatt ttgccgggcg atgaaattat
    10081 tatcagcact atggagcacc acagcaatat tgttccatgg caaatgctgt gtgaagaaaa
    10141 taatgctgtg ctcaaaatta ttcccatgaa cgatgcaggc gaattgatga tggatgagta
    10201 tgaaaaactt ctaacgccaa aaactaaatt agtgtcggta gtacatacat caaattcatt
    10261 gggtaccatt aatcctgtag aaaaaataat tgctctcgct cataaatgtg gtgctgtggt
    10321 gatgatagat ggagcgcaag ccatagtgca cgatgtgata gatgtgcaaa aaatggattg
    10381 cgattttttt gctttcagcg cacataaaat gtgtggccct acaggggtag gtgtgttgta
    10441 tggcaagcaa aatattttag aacaaatgcc tccataccaa ggtggtggcg atatgataaa
    10501 atcggtaacg tttgcaaaaa ccacttacaa cgatataccc ttccgtttcg aagcgggcac
    10561 acaaaacatt gccgatgtga ttgcacttgg tgtggcagtt gattattaca acagtttgga
    10621 tcgtattcaa tgccttgtac atgaaacagc cttgatggaa tatacaatgc aacaattaaa
    10681 acaaattgaa aaaattaaac tcataggtac tgcaactaat aaagccagtg tagtatcatt
    10741 tgtaatagat ggagttaatg ccatggatgc cggcatgtat ctcgatacta tgggcatagc
    10801 agttcgcaca ggacaacatt gtacacaacc cgttatggat caactttgca ttgccggaac
    10861 agtgcgcgca tcgtatatgt tttataatac aatggaagaa gttgataagt tggttgaagg
    10921 agtgaaaaaa gtggttagtg ctttttaaaa ttattgcgac aagattttca tttttttttg
    10981 ttcaccaaaa tgtgtcaata tcttgtaaaa aatatttgcc aatacatcca aaccgagtta
    11041 tttatcgttt ataattgcaa aggtgtataa gatggaatgc tatttgagag acaacaagta
    11101 atttaaaaac aaaaacatga acctgcagca attaataaat aaagaactac caccacttaa
    11161 aacaacagat actgttgcca aggcgctcaa ttggatgcag gttttcaaag tgcgtcagct
    11221 tccggtactt aaagaaaaaa cttttattgg cgtaattgaa gaagaacact tgaaaaaaat
    11281 aagcgatgta aatcttccct tagccgctac cgatgtaaaa atagttcgct catttatgta
    11341 caacgatcag cattattatg gcgcttttac attttgtacc aataaccaac tcgaaattat
    11401 tccggtattg aatcgtgagg gccattatct gggcttaata actgctgcca gcctcatcga
    11461 ctgttttgcc caaataaaat gggcatcgga cgattgcagt atcattgtgc ttgagtgccc
    11521 catgcgcgat tatttattgt cgaaaattat aaacattgcc gaatccaacg gtgccaatgt
    11581 gcttagtgta gatgtaaacc ttatggccga tggcaataca aatgaagtta ccatgcgcct
    11641 caacaacatc gatttggcac ggatcgaagc agggtttttc cgccatgatt ataaaattac
    11701 agcatcgtat tccacagctt tggtcaatcc cgattttcag gatcgttatg attcacttat
    11761 gaaatatttg ggcatttaat cttcaaagca ccttccagtt accgtttatt accaaactta
    11821 tgatttgcgc tatttcctat ttataacaat tataataaat ttgcattttt gaagtttata
    11881 attgttcaaa ttaagcagtt cgaaaaagtg aaaactatac aaacaccggc attgaagtcg
    11941 ttgattcttt tggcatttat tgcaaccatt atatttactg gttcatgttc gcgtaaacgc
    12001 aataccatgg tgagcagggc atggcacaac actacatcgc attacaacgg atattttaat
    12061 gcccgcgaaa aagtacagga agtagcaaaa aaactagcaa cagcacaacc cgataaattt
    12121 gaacgcacat taagtatttt taagcttggt gacgacaatc aagccaaagc aaactatccc
    12181 gatttggatg aggccataaa gaaatcttcc attgttattc aaaagcactc tatcaaaata
    12241 aaaggcaaag aatactgtaa gtggattgat gaaaattatc taatggtagg tctggcgcaa
    12301 ttttacaaac acgattactg gactgccata gaggcatttc aattcactgc ttcggagtat
    12361 aaagaaagca attcgcgcta cgatgccatg atatggctta cgctcactta catgcagctt
    12421 ggcaaaatgg tagatgccga atatgttatc gactttttga ataaccaaag taaaatgacg
    12481 cctaagcaaa agcagttgtt cagtttggta aaggccgatt attacagcaa gaaagatgat
    12541 atacccaatg agataaaggc attggaagag gcagtagcca attcgcgaaa aaaagatgat
    12601 aaagttcgat accgttttat tttggcacag ttgtatcaaa aattgggcga ctcgcgaaag
    12661 gcatatggcc tgtatgaaaa ggttataaaa atgaacccat attatgagtt agcttttaat
    12721 gcaaaaataa atcgtgcccg ggtattcgat gctgaaagtg ccagcggagg aacagtaaag
    12781 aagcagctga acaaaatgtt gcgtgatgat aaaaataaag aataccgcga ccaaatttat
    12841 tatgccctgg caggtattgc aaaaaaagaa agcaaagaat tagaggaaat tgacttgttg
    12901 aaaaaatctg ttgcaaccag cgtaagtaat aacaatcaaa aagcaatatc atacctcgac
    12961 cttggcgaaa tatcattcag taaaaagaat taccgtgcag cacaggctta ctacgatagc
    13021 accatgcaat ttcttacaaa agactatccc acttacgacc aaataagtaa taagaaatcg
    13081 accctcacac gcctggttga aaacatgatg gtgatagaaa atgaggatag tttacaagca
    13141 atagctaagt taagtgaaaa ggaaagggaa gattacatag ctggcattat taaaaaagaa
    13201 aacgaagaaa aagagcgaca aaaattagaa gcagagaaaa agaagcagga ggaaatacaa
    13261 aacgaaaatc aggtaaatca aaccctgaat gaagagaata tccgtaaaaa tacaaaccag
    13321 caaattccag gtgcaggtaa tggagcatgg tatttttata atacagcaac attatctttt
    13381 ggcttcaatg agtttataaa aaaatggggt aaccggccgc tcgaagataa ctggcgcaga
    13441 aaagaaaagg aatcgcaagc actggcaaat agtgaacaag gcgaagataa aatagatatg
    13501 actgcgtata acgattccat ttcgaaactt gccgatgcca agcgcaaaga aatctatttg
    13561 aaaaaattac cggtaacgtc cgaagctctc gttgcatcga atgccaagat tgatgaagcg
    13621 tattacaaca ttggaattat ttatcgcgag caattgaaag agtatcccca atcgcgcgat
    13681 aattttgaag gcttactcga acgttttccc gattcgaaat ttaaattgcc ttcttactat
    13741 aacctgtacc ggttatactc tgcattgggt aatgccgaga aagcagaata ctataaaaat
    13801 cttttgctga caaaatatgc tgatagcgac tatgcaaagt tgattcttaa tcccgaatat
    13861 tttaacaaca aacaacaacg tgccgacatt attcaaacat attatgagaa cacttacaag
    13921 gcatatcaga atcagcagtt tcttgctgta atagaaagaa aaaatgaagc cgattcgttg
    13981 tttccaccta attcgctcac acctaaattc gaattgctaa agtcgttggc aattggcaaa
    14041 accaggccta tcagcgattt tgaactttcg ctacgcgatg tgattgcgca ttatcccaaa
    14101 gacagtgtaa gcatcacggc aaaaaacatt ttggattata tcacaaagaa taatttgaat
    14161 ggaagcaaaa aagccgtaaa ccaagaggtg acagacagtt caaaaacgga aaatatcaat
    14221 cagtcagatt caacacaagc caataatgag ttgccaaaaa cacctttcgc ttttaacccg
    14281 gatactattc actattttgt aatggtgttt gaaaataaaa ccatcaattt aaatgaattg
    14341 aaaataaaaa tatcaaactt caacacaaaa ttttatagtg ttgctggata tcaaattaac
    14401 aatgcattta taggttccga aaaacaatac cttgctgtaa aacaatttac gaaccgcgaa
    14461 gaggcatcgt cttatatgga aacactgctg tcggacgagg aagtatttga tgataaaagc
    14521 aattacgagt atcaggtgtt tttaatatcg ccttcaaact tcggtaagtt gatggtgacc
    14581 aaagatattg aaggttatct caatttttat gatgagcaat actcgcagta atttttggtc
    14641 ttgagtattt ggtattgggt atgctggtat tgggaattga gtatttttgt attgagtatt
    14701 gggtatgctg gtattgggtt ttgagtaatt ggtattgggt tctgagtatt tttgtattgg
    14761 ggatgctggt attgagtaat gagtattttg gatttgtttg tggagcattt tttttgactt
    14821 caaacttcaa actttaaact tcaaacttca aacctcaaac ctcaaacttt tcttcctttc
    14881 ccttttaaat caaattatta tcttcgcagc ctcttaaatt aaattcctga tagcaatatg
    14941 tttagtaaca aaccaaaaac aaacaacaca agtagtgagg caacttcatc ttcaataaac
    15001 cttataggtg ccggtactgt aatagatggg gaaataaaat cgaatggcga tattcgcatt
    15061 gatggtactg taaatggtgc agtgcatagt aaagccaaag tagtagtagg gccaacagga
    15121 gttgttgaag gcgatgttca ttgtcaaaat gccgatatat cgggcgctgt aaaagggaca
    15181 acacatgtat cggacacatg ctttcttaaa gctacgtcac gcattgtagg tgacattgtt
    15241 accggaaaat ttgtggtaga agtaggcgcc aacttcacag gcaattgcaa tatgggtcaa
    15301 gtaaaagaaa taaattatgc cgaccgacaa aaagagcaac cagccatccg acaaaaaacc
    15361 ggttgataat tatattcgct tttcaactat ggggctccaa atgggcgcta ccatttttct
    15421 gtttacatat gccggaataa aactcgatga gtggcttaaa ctttcctttc cggcttttac
    15481 aatttcacta gcattatttt cagttatagc ttctatttat gtggtagcaa agcaagccat
    15541 gaaaggctaa tgcttcatag ttttgtgcgt ttgaaaggtt ccaatatttg tttgaaaaat
    15601 gaatgcgggt attgctccac attttcgaaa cccaacttaa tatcacgacc cgtgtgaggt
    15661 atatatgctg taccaaaaat atagtcccat acacttagcg atagtccata atttacccca
    15721 tacttaccgg caggcaattc ttttgcatga tgccatatat gcattgccgg attattgaaa
    15781 acatatttca atggaccata attcaaagga atgttactgt ggttgagatg tcctaaagcg
    15841 acagcaataa tatgaatgat gaaaaaatcg gaaatgccaa agccaatcat tgccaacgga
    15901 atgtattgca aagttttata aacaatggtt tccatccaat gaaaacgcag gtgtgcagca
    15961 aaacccattt cctctacaga gtgatgcact ttgtgaaact cccacaacct cggaaccctg
    16021 tgcaatagcc tgtgaatgtt ccattgtata aaatcggcaa ttacaaacat caataaaaac
    16081 tgaagaaaaa ccggccaaga ttttatattg atggcaacca aattttcgat gcctatgcgc
    16141 gataggaaat tattgaaaac atcaacagct atatttgaaa tggcattata tccaattaac
    16201 gaaaagagaa aaaaattaaa gaacatataa aagccatcga gccagaaatc tttacgcaca
    16261 actgcctgtg cggttcgcca gggaaaaaca atttcgagta gccatactaa cagcgacaaa
    16321 cccaacagcc aataaaaata attgtgccag ccgggataca atatttcttt aagcagataa
    16381 ttataatagc cgctatacga ttgcgcaata atgtcggaat aatgttgcca cataataatt
    16441 attgttgtga gcgcgatttt ctttcgcgcg cttttatttc aaactctttg attatatttt
    16501 cattctttgg ccccgtgaat accagtgtga atttacccgg ttgcagataa taatttccat
    16561 cgtcagcttt tttcaatatt attttttcat cgcctgtttt gtttttatta accataaggg
    16621 tatacgcagc tacgtttgta gaatcgaaag taaaatgata gtcaataaaa tttatgcctt
    16681 gctcacaagt gtcggtaacg gtgcttaata ataaatcatc ggcttttatt tgcaacttaa
    16741 caataccgga cttgccggca tagtaagcaa atgactgtga gggttttata accggctcat
    16801 attcgccttg cttttgtccc catcgcgaag agtgtttttt attctcaata tcgaaaacat
    16861 aaaagggctt gtttattaat gtatcataaa gttgttgtat taaacttaca tcgccaataa
    16921 atgcactgcg gccatgcgtg cctattaata tttttttatc gcggggatga attgccaaat
    16981 cgtgaatagg tactgcgggc atttcgtttt tccattgcat aaatgttttg cctccgttga
    17041 gcgaaacata taaaccatga tcggtgcctg catagataat attttcgttt gcgggatctt
    17101 ccttgattac attcaccggt tcggcaggta agtccttacc tattggctgc cacgtatttc
    17161 cataatcgtt gcttacaaat atgaatgccg aaaaattatc gaaacggtaa ccattgagtg
    17221 aaacatatac gcgtccttca acatgcgatg atgctgccac ccgcgatacc cagtattttt
    17281 gcggcaatcc atccgatatt tttttccatg tattgccgcc atctttcgat acgtgtatca
    17341 agccatcatc gctgccggta tatagtaatc caaaccgcaa tgatgattca tcgatacagg
    17401 taatggtacc atacggcaca tcgcccttta tgccacccaa agtgagatcg ttacttatgg
    17461 ctttccatgt atcgcctttg ttcattgacc ggtacaattt atttgcgccc atataaatta
    17521 tatcgccctg atgtttgctt attagtatag gcgtttgcca gttccaacgc aaaggccttt
    17581 cgccaagttc aacctgtggt gtaatatatt tttgatcgcc cgttgcaata tttatacgat
    17641 aataatttcc aaattgataa cctgtgtaag caatattatc atcgcgggta tcaatctgaa
    17701 tttgcatacc atcgccaccc atcagtgcct tataagcctg accattatta tcggcataac
    17761 cgtagtcggc tttattttgt tgccactgcc atacaccatt atcctgtaag cccccatata
    17821 aattaaatgg agtttgataa tcgaaattga tgctgtaaaa ttgccccacc ggaggcgtat
    17881 tgcatttaac ataagttgcg ccactatcgt acgaaacatt taacccgcca tcgtttgcaa
    17941 gtattatgtg gccctctctg tttggattaa tataaatgct gtgaaaatct acatgcacat
    18001 tatcggcaag aatagaagag aacgttttgc cgccatttgt cgattttaat atttgaaaac
    18061 cgggcaaata aatttcatca tcattgaaag gcgaaatttt tatttgtcca aaataatatc
    18121 cgtaagtatt gaacaactct tctatatatt tttcgttttg ttttttccac gttgtacctg
    18181 catcgtcact cctgtatact tcaccgcctg taacatcagt atcaaataac aatgagtttg
    18241 catcttctaa atattccacc aaagccgatg gagtaatttt attttcgcgc accatttttt
    18301 ttactgaggc agcattatat ttttcgggaa aattattttc atcgagatag tcatcaattt
    18361 gtttatcgct aagttgtaag aaagtttctt tcgaaatttg acgcaatgta tcgcgcgata
    18421 aaattttatt ttgcgataaa gcttcttccg gtttttttgg cctgcgggtt tgattatcta
    18481 atacagcata aactatattt gaatgcgttg cagaaatggc caacccaatt cttccaatac
    18541 cctctccctg cggaaaccct gattgtgcgc ccgatacaag cttccatata ttgccaccat
    18601 caatcgattt gtatatgccc gatgatgaac cggactcggt aaaattccat gccctgcgct
    18661 cgcgatgcca catagaagtg tacaatatat tttcattctt agcatcaatt atcaaatcaa
    18721 ttgcaccggt gttgtcattt acaaacaaag tgtgtttcca tgtagcacca ccatctgtag
    18781 atttaaaaat tcctctttcg tcattagcag tatataaatg tcctaatgct gccacccaaa
    18841 ttgtgtttgg ttgcgttgga tgtataatta ttcggccaat atgttgtgta ttgggtaagc
    18901 cgatatgttg ccatgttttt cctttatctc tacttttata aacaccaaca ccggagtaag
    18961 atgagcgcga agaattattt tctcctgtac caacatataa tatttcgcca tgctgccaat
    19021 caacggcaat gtcacctatt gtcataaccg cctcgttgtc gaacaacgga gtaaatgtat
    19081 tgccgttatt ggttgttttc cacaaaccac ccgaagcaaa agctacataa aactgagtag
    19141 catcgtatgg agaaacatca atatctacca cacggccagc cattaatgta ggtccaacat
    19201 ttttcaattt cacgttattc aacaacgatg tgctgttcaa ctttttgcgc tgttcaaaag
    19261 catcttcccg cgtttgcgat gttgaaggca atggaaaatc gctcacccct ttcttctttt
    19321 gtgcttttga agttgaagca aaaagcaatg tggcacctat caatgctgtg tagaaaatat
    19381 tttttaccat atgacagtac aatatgtata tttgtggaaa tggagttgta aagaaaacaa
    19441 aagatgaaca aacaatgttg aaatgatttt tgctgcaatc aatattttgc aaatgcgttt
    19501 gagaaacaat gacatcataa tttagctata atacatcaaa cactgacaaa ttcaaaaagt
    19561 gatattgcat attcgacaag caaaaaagtg gttttgatat taaaatatcc cacttcatta
    19621 atattaccgc cttcttggat tgttttattg aaaaaagtgc aacatatttg catcataaga
    19681 aaaaaaacag aaaatgaata gcagattgaa aatattttta gccttcttag ccatcggatt
    19741 tcttggttct tgcaccaatg ataaagcaga tgagattgta cctaatctta attgcgatac
    19801 aacatattat caaaccacca ttaaaccaat tgtaattgca aactgtgcca ttcagggttg
    19861 ccatgttact ggaggccaat cgcctaattt tatttcatat gcaaatattt ttgataacag
    19921 aaataaaatt aagacaaata ttaatttgcc atcaaacgat aataatatta tgcctccgtc
    19981 cggcccgctt agtgctgccg accttactaa aataaacaat tggatagaca gcggtgcagc
    20041 cggctgcgat taataaattg aattttaaaa ttttaaaaaa atgaaaaaat attttttggt
    20101 ttttattgct tgtat
//
LOCUS       DAORQA010000025        19651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_709_length_19651_cov_27.952694, whole genome shotgun sequence.
ACCESSION   DAORQA010000025 DAORQA010000000
VERSION     DAORQA010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 19651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19651
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_709_length_19651_cov_27.952694"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="PK736_03370"
     CDS             <1..1291
                     /locus_tag="PK736_03370"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32465.1"
                     /translation="YTFTVTDANGCTTVVSGTISQPAVLMNTAIPATVACNATTTSVT
                     VTAAGGTSPYTGVGIFTNQTAGMKTYFVTDANGCISSAAINIVIPAIMNVTTTVVNPA
                     CGLSNGTITANASGGVGPYTYLWAGGQTTQIRTGAGSGTYTVTVMDSRGCTKTASTSI
                     SVSGNTPPMPGNINGPVSICKNQTNVIYSIAPVSGATSYNWLVPVGASIIAGQGTTQI
                     TVNFTNFINNGNVRVRSSNTCGASPYKIKAVYAMMKSPVINGLNYACKNQKGLVYSVN
                     PIPGAISYTWTVVPGSTIVSGLNTNSITVNWGSINGTISVKVNSACGVSTPGTLGVAF
                     TCKEGNNNELSNTISPYPVPTDRYTSIDLLSETDGLQTEIYVYNLLGEKVLFETAILQ
                     SGENKITIDCKNLDNGTYIMKVHNKEINKQGRILIEK"
     gene            complement(1277..1414)
                     /locus_tag="PK736_03375"
     CDS             complement(1277..1414)
                     /locus_tag="PK736_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32466.1"
                     /translation="MKKAVIVWQLFSKQDNYLWLRINKNPESSFQDPGRFDNVGDLLF
                     N"
     gene            1505..3367
                     /locus_tag="PK736_03380"
     CDS             1505..3367
                     /locus_tag="PK736_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRC32467.1"
                     /translation="MAQNNTVDSLKQFIQTSKDDSIKVNALILLSKQNNNDNPQLAIQ
                     TAEEAKNIALKIAYKKGFAFAAKNIGLVFYNQGNYVEAINNWQEALKVFDSIGDKTGV
                     ANMLSNQGAVYFNQGDDAKSLELHLQSLKLSEEVNDTLRILTSYTNIGAVYLNKKATY
                     DKALKSFLESYKLSVAINDNYSIGTAAVNLGEVYFKMGDDSTALFYLKRSLEVYEDLE
                     DKPYSLNYVGRVYTKQRDYLLAIQNHKEAYEISKKIDAKLNLAQSSLGMAQAYLGNEE
                     IKSAIPKFEEAVLIAQPLNANMEIKEAYEGLVMCYTKLSDFSNASKYQTLLLNIKDTI
                     YNINTDKKLGTLQFTFDLDKKQGEINLLTKDQEIKDQEIKRQKLVRNGFIGGFAIVAL
                     FAGIFLKQRNRISKEKKRSDELLLNILPAETAEELKATGTAKAKSFDMVTVLFTDFKN
                     FTQASELLSAEDLVKEINYCYSAFDKIITKHNIEKIKTIGDAYMCAGGLPATNSTHPT
                     DVVKAGLEMQEFIARNKKQRQEINMPFFELRLGIHTGPVVAGIVGIKKFAYDIWGDTV
                     NTASRMESSGEVGKVNISGTTYEIIKDQFVCTHRGKIQAKNKGEIDMYFVEKVL"
     gene            3562..5730
                     /locus_tag="PK736_03385"
     CDS             3562..5730
                     /locus_tag="PK736_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC32468.1"
                     /translation="MLFLLNYAAKGQSNSAIDSLKKVLLNSPDDTAKVRTYNTLFSLT
                     WKTGNYKEGLTIAQQNLALSQKLKFQKGIADAYNNIGLMYENMDSRDTALSYYFKSLA
                     ARDTIKNKKQMSATYGNIANLYQARGDYVEAIKYNITALKLKEEIGDSAGMGITYNNV
                     GLVYFNQNNYKEAIANYEKSMSISSLLKNKRNMAMTANNFGTVYQSMNQNDDAIIWLN
                     KAIELNNALGNKSWLSKNYTNLGNSYNSKAAELKKAGNLEGFTTYNQKAMVAFEESLK
                     RLTSDENKDDYMAACNSIGLLCINLGKLIEAESYCKKSLALTTENDNKKIKQETFEFS
                     GLLYNELARQSNISDSLRVIYLKASIDYYEKANELKGQRLNEDNTKQIAEIKTKYETE
                     KKDNEIKLLGKDNELKSASLRQQDFALQLAFLEKEKNKDEIALLNKDKDFQALQLNKT
                     QQELKNRELETKANAAELELTKKDKTIKEKQLSEQTLYRNLLVVGSILLLAFGLLLFN
                     RFRLSKQLESQRALLNERKRISNELHDDLGAQLSTARMFLSSLKNNAEGEKNKTLVEN
                     SIQLIDGSINDLRKIMDDLQVSTLQEKGYLIATEELVNKINLLQQINFSLSHVGLEKR
                     LEQKTEHQLFRITQELINNSMKYAKAKNVYIDLVNRDGKIILLYEDDGIGYDIKDNKR
                     GYGLTNIETRTKSVNGVVEFDSAPGKGARTIIEIPFVYAG"
     gene            5735..6379
                     /locus_tag="PK736_03390"
     CDS             5735..6379
                     /locus_tag="PK736_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC32469.1"
                     /translation="MKLLIADDHRLFVEGLRFILLEELHIEIAGFAINGKEAIEKCNK
                     ENYDVVLMDINMPLIDGIQATLQIKTLHPQIKIMMVSMLSDLPTINKALNAGADAFIL
                     KNTDSGDLLKAFKALSKNEIFISESLAHFFTRDADNSLKTKKEYIQFSENIITPREQS
                     VLKLIVEGYTNQHIADTLFISVKTADTHRKNMLAKLNLSNTASLVKFAIENKLV"
     gene            6517..6723
                     /locus_tag="PK736_03395"
     CDS             6517..6723
                     /locus_tag="PK736_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRC32470.1"
                     /translation="MKVHEILSPREIEIIQQIANGLTDREVGALLEISDKTASTHRKN
                     ILHKLQIKNTALLIRYALENKWVK"
     gene            7062..8780
                     /locus_tag="PK736_03400"
     CDS             7062..8780
                     /locus_tag="PK736_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32471.1"
                     /translation="MKKKSLLNTLILAALFATQLNAQSLLTDSLYGNNGIIQVAVFNG
                     QGGAKNIFRQTDDKILLCGTMYESNCNCNHNFMFRADACGQVDSSFGVNGLVRHKFEF
                     TNIGNDYTLQPDGKILVAGTQAAGNGSSQFHPSISRYNYDGNPDTTFGVMGTNKITNF
                     QNGEFTFLYLLPDGKILCSGSQYTSNHLIMRFDSTGAVDNTFGTNGVVQHATPPGLSL
                     LRSFISAMRSDGKIISVSSDFFNGQLVLSCYDTLGIEDTTFGVNGYIVDPIFIVGGTT
                     KILLQNNDKLIVASRNSAQTAATIARYNTNGSIDTTFGTLGYITVSGAGWLHFVTKLN
                     DDSFIIGYDNGFSCLNCSQFYKYSADGIPDPSFSLDGGNVFTFPGPAFEIADIGITSS
                     NNEMIFGSSHGFNNGKMSLKKYYKAPSLPNITQTGFTLSSNVNNDGATFQWFLNGTAI
                     SNATDSIYTATQPGTYTVEVTNIWGCTLSDAIVLTNTGIETANGLYGVSFYPNPVKET
                     LTIVMPSATINSDYFILDAVGRIVLTGKTLREKTTVDMHSFSPGIYFLKITSLQGNSI
                     VKVLKD"
     gene            8864..12331
                     /locus_tag="PK736_03405"
     CDS             8864..12331
                     /locus_tag="PK736_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRC32472.1"
                     /translation="MKKKFKLKALLIVAIVAMQVCSLNAQTYNAGSGSGTLGINNTFV
                     GSGAGSSAKSAMENNAYFGYRSGFRDTIGEFNAFFGSSAGEDNRGSYNTFIGRGAGFN
                     NTTGFENTFLGWRAGNEQTTASRNVFLGYAAGYYNTTASENVAIGYKALYENQTGTRN
                     VVVGFRADSLSTFTDDVTAVGYEAAKGNTADGTTTFGSRALTSNTNGANNSAFGFDAL
                     RSNTTGANNSAFGNQALKNNTTASFNTAIGARALNANITGSRNTAIGNLTLSLNTTGN
                     NNTAIGDSALRANTTGNDNAAIGRSAMRLNTTGGSNTAMGSFALFTNTIDSNNTAFGF
                     EAANKNTSSGIAAFGSRALRNNTTGLANSGFGNAVLFANTTGQSNSAMGSFAMSSNTT
                     GNSNTAMGDSALQKNTTGNSNAAIGKSAMSSNTTGAFNTAMGSAALATNTTENNNTAF
                     GFEAANQNTSSGIAAFGSGALRNNTTGVSNSGFGNSVLFANTTGQSNSAMGSFAMSSN
                     TTGNSNTAMGDSALQKNTTGNSNAAIGKSAMSSNTTGAFNTAMGSAALAANTTENNNT
                     AFGFEAANKNTSSGIAAFGSGALRNNTTGVSNSAFGNAALIANTTGGFNTALGSFAMS
                     ANTNGSSNTAVGDSALLKNTSGSSNSALGNNSLYNNTTGTGNVAIGFESLFKVSTSGN
                     NSAFGFEAAHENSSAGISAFGFKALRQNTFGVSNSAFGNSAMIANTTGNFNTAIGSFA
                     LTANTSGNSNSAIGDSALIRNTIGSRNTSIGNNSLVANTIGNSNSAIGFYTLAANSSG
                     SFNSAVGDSALYLNTSGTSNVAFGTSALFNNTTGLRNVSIGNNSGTSKSNNQRCTFVG
                     STADASGTGLGRTNATALGFGVAVNANNKMRFGNNAVTVIEGQVAYTIPSDGRFKNNV
                     KEDVPGLAFINKLRPVTYNFDRAKFSKHVGENQDEASYLKQLNEQSKELSTGFIAQEV
                     ERAANDLGYHFDGLHIADKDNANDNYSLAYSQFVVPLVKAVQELDEKQNAAVKQSDFQ
                     KLKEENAQLKNEIETMKSCLETLCNNSEITNTSNLELQTSNYLLQNNPNPFKENTTIG
                     FYISSNSSKGLLKIFSMNGEEIKSFVISTKGKSQIEISGNTLAPGVYTYVLIVDDMTV
                     DTKQMVVTK"
     gene            12429..15005
                     /locus_tag="PK736_03410"
     CDS             12429..15005
                     /locus_tag="PK736_03410"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRC32473.1"
                     /translation="MKKLIILSVAIMFATTMQSIAQNTFPAAGSAGVYTTTPNSSAAL
                     DIDTIGKGLLIPRMTLVQRNAIATPATGLLIYQTNSTPGFYYFSGFTWTAVTPTAGAT
                     KTLNNLTAPTAVSQSLLPSTDNTKDLGSSTLAWKNIFAKGTINTDSIYTINGKPVLSI
                     KGNFNLFVGDSAGLGITSGVNNTAIGSRALRNNTTGSGNTANGSHALFFNTTGFDNTA
                     NGRNALVSNTSGFFNTAIGLGALSDNTTGSSNSANGHGALSANTTGISNTTNGRNSLF
                     SNTTGNSNTANGRDAGFSNTTGSNNTYIGTNANASLNNLNNATAIGSDAFVGASNSLV
                     LGSIAGVNGAANSVNVGIGTTTPAFKLDVKEGSINTDSIYRINGKQVLSIKGINNLFV
                     GANAGILNTIGDNNTANGSGALGNNTTGSENTAIGHSALALNTTGEYNTASGWSALES
                     NTNGVYNTAFGHEALESNTTGDFNTANGRGALISNTTGDDNTANGSGTLFSSTIGENN
                     TANGKNALFFNTTGSNNTASGSSAGRNNVSGSHNTFVGDSSTASLNNLINATAIGSHA
                     TVGASNCMVLGSIAGLNGATSSVNVGIGTTVPAFRLQISTNSAAKPTSNTWTIASDAR
                     LKTNVHDYKEGLDALKKIHPVWFTYTGEAGMPKETGVGVLAQELKEVAPYMVGTWNYN
                     DDNNKSTDYLSVDNGAMTYMLINSVKELDAELKIENEKLQNENANQQLAIGNLQNENQ
                     VLKNEISEMKSCLETLCNSSEQTKTNSQQLEANSYLQQNQPNPFNTKTAINYFVAEQN
                     AKAILIIRDLNGSALKEFTLVQSGKGSVTVNANELATGTYTYSLFVNDTCIDTKLMVV
                     TK"
     gene            15239..16477
                     /locus_tag="PK736_03415"
     CDS             15239..16477
                     /locus_tag="PK736_03415"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRC32474.1"
                     /translation="MAIFYFIAVKCYGLFIAVGSIFNDKARQWMRGRKGLFSLMRTTL
                     KPDEQRIWIHCSSLGEFEQGRPVMERMRQEFPQFKLVVTFFSPSGYKAKKLDTIADYV
                     FYLPLDGPHNAKRFLDLVKPHAAFFVKYEFWHFYIYECKRRKIPSYAFSCNFRPSQVY
                     FQWYGFFFDKILRRISHIFVQNQRSLELLYKRSIPHVTVSGDTRFDRVVATRDNNRSL
                     EIVEIFHQNKKVFIAGSTWPADDDIICKLINDCTDDYKFIIAPHEISETKLSELLQKI
                     KKSAVRYSQFTIDNKECEVMIIDNIGLLSTLYRYGTITYVGGGFGTGIHNILEPAVYG
                     LPVIVAPQYSKFNEALALLKIRGMFVVKNHQTLKTVFDDLKYNFEKLQAIKTANLKYI
                     DQQKGATDIIINYLKLNWEE"
     gene            complement(16763..17464)
                     /locus_tag="PK736_03420"
     CDS             complement(16763..17464)
                     /locus_tag="PK736_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018674563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRC32475.1"
                     /translation="MIIKTVKDAATATADIKSGMTLLVGGFGLCGIPENCINAMVAMG
                     INNLTCVSNNAGIDDFGLGLLLQNKQVKKMISSYVGENAEFERQMLSGELQVDLIPQG
                     TLATRCMMSGYGIPFFPTPAGVGTEVAEGKQIINWKGRDYLMEEAFDADFAIVKAWKG
                     DTTGNLIFRATARNFNPLMAMAGKITIAEVEHLVQPGELDPNEIHVPGIYVHRIFQGA
                     NYEKRIEQLTSRKRL"
     gene            17668..18198
                     /locus_tag="PK736_03425"
     CDS             17668..18198
                     /locus_tag="PK736_03425"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32476.1"
                     /translation="MKQFLANLFYLFLSCTLIISCGGKFEFEKQGRKPVYVSYDNLFQ
                     YSQTVPQPIQNSGKILLYGNYLFMIEVNKGVHVIDISDTANPQKMSFLNIPGNKDITS
                     QNNFLYADNGPDLLVLDLSDINNIKLITRQKSVFKPSEFYPSGFIGFFECADYAKGWV
                     IAWETALLQNPECRRE"
     gene            18264..19019
                     /locus_tag="PK736_03430"
     CDS             18264..19019
                     /locus_tag="PK736_03430"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32477.1"
                     /translation="MKNILKAVLICSIIFSACTKENQSVTADRTAQGTNGSLTRFAIK
                     DNFMYALDVNYMKVFDISTGSDPILVNSVKINYGLETITIYGEYVYIGAHDRIYMVGI
                     NDPSQPLEYDEMQHNISCDPVVVQGNYAYSTQNSFEVGCGHATSQSILAVYNISNPQQ
                     TNLLKSITMTSPYGLAVEGKNLYVCDSEEGVIVYNISNPESPQALSTIPVANSRDIIL
                     NFPYMIISTQTSFEMYNYQDVNNIYHVSTFQLK"
     gene            19020..>19651
                     /locus_tag="PK736_03435"
     CDS             19020..>19651
                     /locus_tag="PK736_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32478.1"
                     /translation="MRQAFFVVILLFCFHKLFSQDTPSYSKFSLVTRPLAWVDPVNQS
                     FSVGLQYRVSQKCIVEVQPGIIHSAYQNLIVDSFNVHYAGFRGSAEAMFFIEESPYYV
                     SCEYLYKNFTRSKDEWMLRMGSTYQQKFRVDKYYRVSSYRFKAGVTTNRNGSRVHLDL
                     AAGIGLRTKNVSFSPLPEDAEFLEQSNFENETVAGRSFVPELFFTISMVY"
BASE COUNT         6766 a         3502 c         3732 g         5651 t
ORIGIN      
        1 gtatacattt accgttaccg atgccaacgg ttgcacaact gttgtgagtg gcacaattag
       61 tcagccagct gttttaatga acacagctat ccctgctaca gttgcatgta atgccaccac
      121 tacaagcgtt acggttacag ctgcgggcgg tacctctcca tataccggag taggaatatt
      181 tacaaatcaa actgcaggta tgaaaaccta ttttgtaaca gatgcaaatg gatgtatttc
      241 atcagctgca attaatattg tgattccggc aataatgaat gtaacaacta ctgttgtgaa
      301 tccggcatgt ggattaagta acggaaccat tacagcaaat gcatcgggtg gagtgggacc
      361 ctatacctat ttatgggccg gtggacaaac cacacaaata agaaccgggg caggcagtgg
      421 aacgtatact gttacagtta tggattcacg aggttgtacc aaaacggctt ctacttccat
      481 aagcgttagt gggaatactc cccctatgcc gggaaatata aacgggcctg taagcatttg
      541 caaaaaccaa acgaatgtaa tttattcgat agcaccagtg tcaggcgcaa catcctataa
      601 ttggctcgta cctgtcggtg cttctattat agcagggcaa ggcactacac agattactgt
      661 aaatttcaca aactttatca ataatggcaa tgtgagagtg cgttcttcca atacatgtgg
      721 agccagtccg tataaaatta aagctgttta tgccatgatg aaatcgcccg ttattaatgg
      781 gttaaattat gcctgtaaaa accaaaaagg tttagtgtat tccgttaacc ctattcccgg
      841 agctatttcg tatacatgga ctgttgtacc cggcagcact attgtaagcg gattaaatac
      901 aaattcgata actgtaaatt ggggtagtat aaatggtacc atttcggtaa aagtaaatag
      961 tgcatgtggt gttagcactc cgggtactct gggcgtagca ttcacttgta aggaaggaaa
     1021 caataatgag ttgagtaaca ccatttctcc atatccggtt ccaaccgaca ggtatacaag
     1081 tatcgattta ttgagtgaga ccgatggctt acaaactgaa atttatgtat acaatttact
     1141 gggagaaaaa gtgcttttcg aaactgctat attgcaatcg ggcgaaaaca agattaccat
     1201 tgattgtaaa aatttagaca atggaactta tataatgaaa gtgcataaca aagagattaa
     1261 taaacaagga cgtattttaa ttgaaaagta aatcgcccac attatcaaaa cgccccgggt
     1321 cttggaaact tgattcgggg tttttattta ttctaagcca taagtaatta tcctgcttcg
     1381 aaaataattg ccatacaata actgcttttt tcatactttg gcacagccaa tttttatcag
     1441 attgaacaga aaatatttta agcttttttt tattgccgta gtttttattg gcagtgaatc
     1501 tttattggct caaaataata cagttgatag tctcaagcaa tttatacaaa ccagcaaaga
     1561 cgattcaatt aaagttaatg cactcatttt attaagcaaa cagaataata acgataatcc
     1621 tcagttagct attcaaactg ccgaagaggc gaaaaatatc gccttaaaaa tagcttacaa
     1681 aaagggcttt gcctttgcag ctaaaaatat tggtcttgtt ttttacaatc aaggcaacta
     1741 tgtggaggca attaataatt ggcaagaagc attaaaagtt tttgattcta ttggcgataa
     1801 aactggcgtt gccaacatgc tgagtaatca aggtgcggtt tattttaatc aaggcgatga
     1861 cgctaaatcg ttggagttgc acctgcaatc attaaagtta tcggaggaag taaacgacac
     1921 actccgaata ttgacttcct atacaaacat tggtgctgta tatttaaata agaaagccac
     1981 ttacgataaa gcactaaagt cgtttttaga atcttacaaa ctaagtgttg caatcaacga
     2041 taattactca attggtactg cggcagtaaa tttaggagag gtgtatttta agatgggaga
     2101 tgactctact gcattgtttt acttaaaaag gtcgttagaa gtttatgaag acttagaaga
     2161 caaaccatat tctctaaact atgtaggaag agtttatact aagcaaagag attatttgct
     2221 ggcaatacaa aaccacaagg aagcttatga aatttcaaaa aaaatagatg ccaaattaaa
     2281 tttagctcaa tcatcgctgg gtatggcgca agcttattta ggtaatgaag aaataaaatc
     2341 cgccatccca aaatttgaag aagcagtact aatagcgcag ccattaaatg caaatatgga
     2401 aataaaagaa gcatacgaag ggttggtaat gtgttacaca aaattgtcgg atttttcaaa
     2461 tgcttcaaag taccaaacat tattgctcaa cataaaagat accatatata acattaatac
     2521 agataagaaa ttaggaacgc tacaattcac tttcgacctg gataaaaaac aaggcgaaat
     2581 aaatttgctt accaaagatc aggaaataaa agatcaggaa ataaagcgac aaaaattagt
     2641 gcgaaacgga tttattggag gttttgcaat tgtagcgttg tttgcaggaa tatttttaaa
     2701 acaacgtaat cgtatcagca aagaaaaaaa acgtagcgat gagttgttgt taaacatctt
     2761 gcctgcagaa actgccgaag aacttaaagc aaccggtacc gccaaggcaa aaagttttga
     2821 tatggttacc gtattattta ccgattttaa aaattttacg caagccagcg aactccttag
     2881 tgccgaggat ttggttaaag aaattaatta ttgctatagt gctttcgaca aaataattac
     2941 aaagcataat atcgaaaaaa taaaaaccat tggcgatgct tatatgtgtg ccggtggatt
     3001 gcctgcaaca aactcaaccc atcccactga tgttgtaaaa gccggattag aaatgcaaga
     3061 atttattgcc cgaaataaaa aacaacgcca ggaaataaat atgccatttt ttgaacttcg
     3121 actaggtata cataccggac ctgtagtggc aggtattgta ggtataaaaa aattcgccta
     3181 cgatatttgg ggcgatactg taaacacagc ctcacgtatg gaaagcagtg gagaagttgg
     3241 taaagtaaac attagcggta ctacctacga aattattaaa gaccaatttg tatgcaccca
     3301 ccgcggaaaa atacaagcaa aaaataaagg cgaaattgat atgtattttg tggaaaaggt
     3361 tttgtgagat gagttctaaa attaaaatgg tttgaattat caaaaattcg ttgcatttta
     3421 ttattaatcc ggatgattga aattggaaca ttataaataa aatttttcga acaaatattt
     3481 taatcttgta aaaaaataag tttcatattg aaagggaaaa attgtttttt caataaagtt
     3541 gcgcgtttca cttttgttat agtgctgttt cttttgaact acgcagcaaa gggacaaagt
     3601 aattctgcta ttgattcgct taaaaaagtg ctgttgaatt cacctgacga tactgctaaa
     3661 gtgagaactt acaacacatt attttccttg acatggaaaa ccggaaacta taaagaagga
     3721 ctaaccattg ctcagcaaaa tctggcgctt tcgcaaaaat taaaatttca aaaaggcatt
     3781 gccgatgctt ataataacat tgggctgatg tatgaaaata tggactcgag ggatactgct
     3841 ctcagctatt atttcaagtc cttagcagcg cgggatacaa taaaaaacaa aaaacaaatg
     3901 tctgctacct atggtaacat tgcaaactta taccaagcca gaggcgacta tgtagaagca
     3961 ataaaataca atattactgc actaaaacta aaggaggaaa ttggcgattc agcaggtatg
     4021 ggcataactt acaataatgt cggattagtt tactttaatc agaataatta caaggaagcc
     4081 attgctaatt atgaaaaatc tatgtccatc tcaagcctat taaaaaataa aagaaacatg
     4141 gcaatgaccg cgaacaattt tggcactgtt tatcaaagca tgaatcaaaa tgacgatgct
     4201 attatttggt tgaataaagc gattgaatta aacaatgcac taggaaacaa aagttggtta
     4261 agtaaaaact atacaaacct tggcaactct tataactcaa aagctgccga attaaaaaag
     4321 gcaggcaatc tcgagggctt tactacctat aatcaaaaag caatggtggc ttttgaagaa
     4381 tctcttaaaa gactgacgtc tgatgaaaac aaagatgatt acatggcagc ttgtaatagc
     4441 attggccttt tatgcataaa tttaggaaag ctaatagagg ccgaaagtta ttgtaaaaaa
     4501 tcgttagcac tgacaactga aaacgacaac aaaaaaataa aacaggaaac atttgagttt
     4561 tcaggattat tgtacaacga acttgcaagg caaagcaata tcagcgactc tttaagagta
     4621 atttatttaa aagcatcaat tgattattat gaaaaggcga atgaattaaa gggccaaaga
     4681 ctcaatgaag acaacacgaa acaaattgca gaaattaaaa ccaaatacga aaccgaaaaa
     4741 aaagacaatg aaattaaatt attgggtaag gacaatgaat taaaatcggc cagtctgagg
     4801 caacaggatt ttgccttgca attggctttt cttgaaaagg agaaaaataa ggatgaaatt
     4861 gcattgctta ataaagataa agactttcag gcattgcaat taaacaagac acaacaagag
     4921 cttaaaaacc gtgagttgga aaccaaagcc aatgctgcag agttggaatt gactaaaaaa
     4981 gataaaacga taaaagaaaa acaattaagc gaacaaacgc tctatcgaaa tttattagta
     5041 gttggttcaa ttcttttact tgcttttgga ttgttgttgt ttaatcgttt caggttaagc
     5101 aagcaattag aaagtcaacg tgcattgctt aacgaacgca agcgaatcag caatgaatta
     5161 catgacgacc tgggcgcaca actttcaaca gcacgtatgt ttttaagtag cttaaagaac
     5221 aatgctgaag gagaaaaaaa caaaacactg gtcgaaaatt ccattcaatt aatagatggt
     5281 tctataaatg atttgcgaaa aataatggac gacttgcaag tttctacact tcaggaaaaa
     5341 ggatatttaa tagctaccga agagctggta aataaaatca atttgctaca gcaaataaat
     5401 ttttctttat ctcatgtagg tttagaaaaa cgactggaac aaaaaaccga acatcaactt
     5461 ttccgtatta cccaagagct cattaacaac tcgatgaagt atgcaaaagc taaaaatgtt
     5521 tatattgatt tggtaaatcg agatggaaaa ataattttgc tttacgaaga tgatggcatt
     5581 ggttacgata taaaagacaa caaacgcggt tatggtttaa ccaatattga aacgcgaaca
     5641 aaaagtgtaa acggtgttgt tgaatttgac tcggcaccag gcaaaggagc tcgcactatt
     5701 attgaaatac catttgttta tgctggataa aaaaatgaaa ttattaattg ccgatgacca
     5761 caggcttttt gttgaaggac ttcgatttat tttgctggaa gaattgcata ttgaaattgc
     5821 gggctttgca ataaatggaa aagaagctat tgaaaaatgt aataaagaaa attatgatgt
     5881 ggtgttgatg gatattaata tgcctttgat tgatggcata caagctactt tgcaaataaa
     5941 aacactgcat ccacaaatca aaataatgat ggtaagtatg ctgagcgatt tgccaaccat
     6001 taataaagca ttaaatgccg gtgccgatgc tttcatttta aaaaacaccg acagtggtga
     6061 tttattaaaa gcgtttaaag ccctgagtaa aaatgaaatt tttatttctg aatctcttgc
     6121 acattttttt acacgcgatg cagacaactc cctgaaaaca aaaaaagaat acattcaatt
     6181 ttccgaaaac attatcacac cacgcgagca atcggtgtta aaattaattg ttgaaggtta
     6241 taccaatcag catattgccg atacgctttt tatctcggta aaaactgccg acacacatcg
     6301 caaaaacatg ctggctaaat tgaacctttc caacactgca tcattagtaa agtttgctat
     6361 cgagaataag ttggtttgat gacaacttgc ttttaatcat tcttataaac taatgaaccc
     6421 ataaactatt taacttataa actctcaaat aaaatataca ccttttagtg tattgcatgg
     6481 tcattaagca gcaatcattt ttgcacaaaa aaaaatatga aagtgcacga aattttatcc
     6541 ccgcgcgaaa tcgaaattat acaacaaatt gccaatggac ttaccgaccg cgaagtgggt
     6601 gcattgttag agataagtga taaaactgcc agcacccatc gaaaaaacat tttacacaaa
     6661 cttcaaatta aaaacacagc gttgctaatt agatatgcat tggaaaacaa atgggtgaag
     6721 tagcttctcg ttttgagtat ttatgcaact aacattaaat tacaagctta aaaaaaatat
     6781 ttaagtgaag ctcaacagga ttttgttttt aaaagaattt caacaacgat aaaagttcat
     6841 atttcttgca tccgagtcat ccttctaatt aaaaaacata acaacaatta tgattgcagt
     6901 ttggcacttc aaagaatgtt ccatttacct ttgtcgtgat taaacaatta atgggagcaa
     6961 aataatttga atatacacct tttggtgtat tgatatcagt ttcacatgca accatctttg
     7021 ccataaatta tcttactaat taaaagtttt taaaaaaaaa tatgaaaaaa aaatcactac
     7081 taaacacatt aattcttgca gctttatttg caactcaact aaatgcacaa tcactcctaa
     7141 ccgattctct ttacggcaac aacggcatta tacaggtagc cgtttttaac ggacagggtg
     7201 gcgcaaaaaa tatttttcgg caaacagatg ataaaattct gctttgcgga acaatgtacg
     7261 aatccaactg caattgcaat cataacttta tgttcagagc cgatgcctgc ggacaagtgg
     7321 atagcagttt tggtgtaaac ggattggtaa gacataaatt tgaatttacc aatataggta
     7381 atgattatac attacaaccc gatggtaaaa tattagttgc cggtacgcaa gctgcgggca
     7441 atggcagtag tcaatttcat ccctctatat ctaggtataa ttatgatggg aatcctgata
     7501 ccacttttgg agtaatgggt accaataaaa tcaccaattt tcaaaatgga gaatttactt
     7561 ttctatacct gttgcctgat ggtaaaatac tttgttcagg tagccaatat accagcaatc
     7621 atttaataat gcgctttgat tcaacaggag cagttgacaa tacttttgga actaacggag
     7681 tagttcaaca tgcaacacca ccagggttga gtttgttaag gagttttatc agtgcaatgc
     7741 gcagcgatgg aaaaatcatt tctgtttctt cagatttttt taacggccag ttggtactca
     7801 gttgttatga tacattagga attgaagaca ctacctttgg cgtaaacgga tatattgttg
     7861 atccaatctt tatagtagga ggcaccacta aaattttatt gcaaaataac gataagctca
     7921 ttgttgccag tcgtaatagc gcccaaactg cagcaaccat tgcccgatac aataccaacg
     7981 gaagcattga caccactttt ggaactttgg ggtatataac cgtaagcgga gccgggtggc
     8041 tgcatttcgt taccaagtta aatgacgatt cctttattat tggatatgac aatggttttt
     8101 catgtttaaa ttgttctcag ttttataagt atagtgccga tggcattcct gacccttcat
     8161 tttcattgga tggtggcaat gtttttacat ttccagggcc tgcatttgaa attgccgata
     8221 ttggaataac ctcttcaaac aatgaaatga tatttggctc gtcccatggt tttaacaatg
     8281 gcaaaatgag cttaaaaaaa tattataaag caccatcact tcccaatatt acacaaacag
     8341 gttttacttt aagttcaaat gtaaataatg atggtgccac ttttcaatgg ttcttaaacg
     8401 gaacagccat aagcaatgca accgacagta tttatacagc aacgcaaccc ggtacctaca
     8461 ccgttgaggt aaccaacatt tggggatgta ctttaagcga tgcaattgtt ttaaccaata
     8521 ccgggattga aacagcaaat ggtttatacg gtgtttcgtt ttatccaaat ccggttaaag
     8581 aaacccttac tatagtaatg ccatctgcaa ctattaattc agactatttt attttagatg
     8641 ctgtaggaag aattgtatta accggaaaaa cgcttcgcga aaaaacaact gttgatatgc
     8701 attcgttttc gcccggaatt tattttctaa aaattacttc attgcaaggc aacagcatag
     8761 taaaggtgct gaaggactaa cttatcttct actaaacccg cgaggtttta aaaacttcgc
     8821 gggttttaaa aaaacaaaaa aactttaaaa aacaaatatc attatgaaaa agaaattcaa
     8881 attaaaggca ctattaattg tagctattgt agctatgcag gtttgtagtt taaatgcaca
     8941 aacttacaat gccggctcag gttcaggcac attgggcatt aacaatactt ttgttggatc
     9001 tggtgcgggc tcttctgcca agtcagcaat ggaaaataat gcgtattttg gttatcggag
     9061 cggttttcga gatacaattg gggaattcaa tgcttttttc gggagttcgg ctggagaaga
     9121 caatagaggc agttacaata catttattgg aagaggggcg ggatttaata acacaacagg
     9181 atttgaaaat acttttctgg gttggagagc aggcaatgaa caaacaaccg caagtagaaa
     9241 tgtttttttg gggtatgcag ccggctatta taatacaacc gcttctgaaa atgttgcaat
     9301 tggctacaaa gcactatatg aaaaccaaac gggaacaaga aatgtagtag tgggttttag
     9361 ggcagactcc ttaagtacct ttacagacga tgttactgca gtggggtatg aagcagctaa
     9421 aggcaacacg gcagatggga ctactacttt cggaagcagg gcgttaactt caaatacaaa
     9481 tggtgcaaat aattcggcat tcggatttga tgcacttaga agtaatacta ctggtgctaa
     9541 taattcggca tttggaaatc aagcattaaa aaacaatacc actgccagct ttaacactgc
     9601 cataggcgca agagcattga acgcaaatat aacgggcagc agaaatacag cgattggtaa
     9661 tttaaccctg tcattaaaca ccacaggaaa taacaataca gcaattggag attctgccct
     9721 tcgagcaaac accactggaa atgacaatgc agctattggt aggtcggcaa tgcggttaaa
     9781 cacgaccgga ggttcaaata cagcaatggg ttcgtttgct ttattcacaa atactattga
     9841 cagtaataac actgcattcg gatttgaagc cgcaaataaa aataccagtt ctggcatagc
     9901 ggcatttggt tcgcgtgcgt tgcgcaataa cacaacagga ttagcaaatt caggttttgg
     9961 taatgctgtg ttgtttgcaa atacaactgg acaatccaat tcagcaatgg gttcatttgc
    10021 catgtcatca aacacaaccg gcaatagcaa tactgcaatg ggagattctg ctttgcaaaa
    10081 aaatacaaca ggtaacagca atgctgcaat tggtaaatca gcaatgtcat caaatacaac
    10141 cggagcattt aatacagcaa tgggctcggc tgctctggca accaatacaa cagaaaacaa
    10201 caacactgca tttggttttg aagccgcaaa tcaaaatacc agttcaggca tagcggcttt
    10261 tggttcgggc gcattgcgca ataacactac aggtgtttca aattcaggtt ttggtaattc
    10321 ggtattgttt gcaaatacaa cgggacaatc caattcagca atgggttcat ttgccatgtc
    10381 atcaaacaca accggcaata gcaatactgc aatgggagat tctgctttgc aaaaaaatac
    10441 aacaggtaac agcaatgctg caattggtaa atcagcaatg tcatcaaata caacaggagc
    10501 atttaataca gcaatgggct cggctgctct ggctgccaat acaacagaaa acaacaacac
    10561 tgcatttggt tttgaagccg caaataaaaa taccagttct ggcatagcgg cttttggttc
    10621 gggtgcattg cgcaataaca ctacaggtgt ttcaaattca gcttttggta atgctgcatt
    10681 aattgccaat actaccggtg gatttaatac cgctttaggt tcttttgcta tgtctgcaaa
    10741 tactaacggt agcagtaata cagctgtagg cgattctgcc ttgttaaaaa acacttcggg
    10801 cagcagtaat tcagcattag gaaataactc attatataac aacacaacag gaaccggtaa
    10861 tgttgcaatt ggatttgaaa gtttattcaa agtttcaaca agtggaaata actctgcatt
    10921 cggttttgaa gcagcacatg aaaacagcag tgccggtata tctgcattcg gattcaaggc
    10981 actcaggcaa aatactttcg gtgtttcgaa ttctgctttt ggtaattctg ctatgattgc
    11041 aaatacaacc ggaaatttta ataccgccat aggctcattt gctttaacag caaatacaag
    11101 cggcaacagt aattcggcaa ttggcgattc tgcccttatc agaaatacaa taggtagccg
    11161 taatacaagt attggaaata attcattagt tgccaacaca ataggaaatt cgaattctgc
    11221 tattggattc tatacattag ctgcaaattc ttcaggaagt tttaattcag ctgttggaga
    11281 ttcagcactc tacttaaaca cttccggaac tagcaatgtt gcatttggaa caagcgcttt
    11341 gttcaacaat acaacaggat taagaaacgt atcaattgga aacaattcag gcacttcgaa
    11401 aagcaacaac caacgatgca catttgtagg tagcacagcc gatgcatcag gaaccggatt
    11461 aggtcgcact aatgccacag cgcttggttt tggtgtagca gttaatgcaa ataataaaat
    11521 gagatttggt aataatgctg ttacagtaat tgaaggacag gtggcatata ctattccaag
    11581 tgatggccgt tttaaaaata atgtaaaaga agatgttccg ggattagcgt ttattaacaa
    11641 actgcgtcct gttacttaca atttcgaccg tgctaaattc agcaaacacg taggcgaaaa
    11701 tcaggatgaa gcatcttact taaaacaact aaacgaacaa agtaaagaac tcagtactgg
    11761 ttttattgct caggaagtgg agcgtgctgc aaatgactta ggatatcact ttgatggttt
    11821 gcatattgca gataaagata atgcgaatga taattattca ttagcataca gtcagtttgt
    11881 tgttccatta gtaaaggcag ttcaggagtt agatgaaaaa caaaacgcag cagtgaagca
    11941 atccgatttt caaaaattaa aagaagaaaa tgcgcaattg aaaaacgaaa tcgaaaccat
    12001 gaaatcatgc cttgaaacac tttgtaacaa tagtgaaata acaaacactt ctaacctcga
    12061 acttcaaact tcaaactatc ttctccaaaa caatcccaat cctttcaaag aaaacacaac
    12121 cattggtttt tacatttcat caaattctag caaaggattg cttaaaatat tttcgatgaa
    12181 tggtgaagag ataaagtcgt ttgtgatttc tacaaaagga aaaagtcaaa ttgaaatttc
    12241 aggcaataca cttgcaccgg gtgtttatac ttatgtgttg attgtagatg atatgactgt
    12301 tgatacgaag cagatggtgg taactaaata atccactcac cccttcaagg gttttaaacc
    12361 cttgaagggg tttattataa aaaggaatat ttaattttaa ttaaacgctt taaacaaaaa
    12421 aaatcattat gaaaaaatta atcatcttgt ccgttgcaat aatgtttgca accaccatgc
    12481 aatcaattgc acaaaacaca tttcctgctg cgggcagtgc gggagtttac accacaacgc
    12541 ccaatagctc ggcagcattg gatattgata ccattggcaa aggtctgctt attccgcgta
    12601 tgacccttgt gcaacgcaat gcaatcgcaa cacctgcaac aggcttgctc atttatcaaa
    12661 ccaacagcac accgggtttt tattatttct ccggattcac gtggacagca gttacaccaa
    12721 ccgcaggtgc gaccaaaaca ttaaacaatt taactgcgcc aacagcagta agtcagtcat
    12781 tacttccatc aactgataat acaaaagact taggcagcag cacgctagca tggaaaaata
    12841 tttttgcaaa gggaactatt aataccgatt cgatttacac cattaatgga aagccagttt
    12901 taagtattaa aggaaacttt aatttgtttg tgggtgatag tgcaggttta gggattactt
    12961 ctggtgttaa taatacggcc attggtagcc gcgcacttcg caataatact actggaagtg
    13021 gtaacacagc aaatggtagc catgctcttt tttttaatac aactggtttt gacaacactg
    13081 ctaatggtag aaatgcactg gtatcaaaca cctcaggatt tttcaacaca gctattggtt
    13141 tgggtgcgct ttctgataac acaacaggaa gtagcaactc agcgaacggt catggtgcac
    13201 tttcagcaaa caccaccggg attagtaata caacgaatgg taggaattca cttttctcaa
    13261 acacgacagg aaatagtaac actgccaatg gtagagacgc aggttttagt aacacaactg
    13321 gttcaaacaa cacatacatt ggaacaaatg ccaatgcctc acttaataat ctaaacaatg
    13381 ctacagccat tggctcagat gcatttgttg gtgcaagcaa tagcttggtg cttggcagta
    13441 ttgcaggagt taatggagca gcaaatagcg taaatgttgg tattggaaca actacacctg
    13501 catttaaatt agatgtaaaa gagggtagca tcaataccga ttcgatttat cgaataaatg
    13561 gaaagcaagt gctaagtata aaaggaatca ataatttgtt tgtgggcgcc aatgcaggta
    13621 tacttaatac cataggtgat aataatactg ccaatggaag tggcgctctt ggcaataaca
    13681 ccactggttc tgaaaacact gcgattggtc atagcgcgct tgccttaaat actactggcg
    13741 aatataatac agcaagtggt tggagtgcac ttgaatcaaa caccaatgga gtttataata
    13801 cagcatttgg tcatgaggcg cttgaatcaa acactacagg tgattttaac acagcaaatg
    13861 gtagaggggc gcttatctca aacactacag gagatgataa cacagcaaat ggcagtggca
    13921 cgcttttctc aagcaccata ggagagaata atactgcgaa tggcaagaac gcacttttct
    13981 ttaatactac gggtagcaat aacacagcaa gcggtagtag tgctggtcgt aataacgtaa
    14041 gtggctcaca caacactttc gttggtgata gttccaccgc atcgctaaat aacttaatta
    14101 atgctactgc aataggctcc catgctacag ttggagcaag caactgtatg gtattgggca
    14161 gcattgcagg attaaatggg gcaacaagta gtgtgaatgt aggtatagga acaactgtac
    14221 ctgcctttag gttacaaata tcaaccaatt cagcagcaaa accaacttca aatacctgga
    14281 ccattgcaag cgatgcacgt ttaaaaacca atgtgcacga ttacaaagaa ggattagatg
    14341 cattaaaaaa aatacatcct gtttggttta cctacaccgg tgaagccggc atgccaaaag
    14401 aaacaggcgt gggagttctt gcacaggagt tgaaagaagt tgcaccttac atggtaggta
    14461 cctggaatta caacgatgac aataataaat caacagatta tttaagtgtt gacaatgggg
    14521 ctatgactta catgctgatt aacagtgtga aggagttgga tgcagaattg aaaattgaaa
    14581 atgaaaaatt gcaaaatgaa aatgcaaatc agcaattagc aataggcaat ttgcaaaatg
    14641 agaatcaggt tttgaaaaat gaaatttctg aaatgaaatc atgccttgaa acactatgta
    14701 atagttccga gcaaacaaaa actaacagcc aacagcttga agctaacagc tatctgcaac
    14761 agaatcaacc caatcctttc aacacaaaaa cggcaataaa ttatttcgtt gcagaacaaa
    14821 acgcaaaggc aattttaatt atccgcgatt taaatggaag cgctttaaaa gagtttacac
    14881 ttgtgcaatc aggaaaagga agtgtaacag taaatgcaaa tgaactggca acaggtacat
    14941 acacctattc tctttttgta aatgatacat gtattgacac taagttgatg gtggtaacta
    15001 aataaaagcc tacccaaacc ctcccgaaaa tgaagacata aatacaagca gcgggtttat
    15061 taaaagattc ccgctgctta acttttaaaa tcaaattaat ccgaattaca aaaaagccat
    15121 tcaattgagc gtcaaccaaa gtatttgaaa ataaagccat tattcgcaat gtagatggct
    15181 gcaaacacaa aaaaaatttt tcctttgcac gcctactgct aaaagtgctg aatacctgat
    15241 ggctattttt tatttcattg ctgtaaaatg ttatggctta ttcattgccg ttggatctat
    15301 attcaacgac aaggccaggc agtggatgcg cggccgtaaa ggattgttca gtcttatgcg
    15361 cacaacgctg aagcccgatg aacagcgtat ttggatacac tgttcatcct tgggtgagtt
    15421 tgaacaaggc aggcctgtaa tggagcgcat gcggcaagaa tttcctcaat ttaaactggt
    15481 agttacattt ttttctcctt ctggttataa agccaaaaaa ctagatacca ttgccgatta
    15541 cgttttttat ttgcctcttg atggaccgca taatgcaaaa aggtttttag atttggtaaa
    15601 acctcatgca gcattttttg taaagtatga gttttggcat ttttatattt atgagtgcaa
    15661 gagaagaaaa attccttcct atgcattttc atgcaacttt cgtccatcac aagtttattt
    15721 tcaatggtat ggatttttct tcgataaaat tttgcgcaga atttcacaca tatttgtgca
    15781 aaatcaacgc tcgctcgaac tgttatacaa acgcagcata ccacatgtta cggtaagtgg
    15841 cgatacacgt ttcgataggg tggtggccac acgcgacaat aatcgctcgc tcgagatagt
    15901 agagattttt catcaaaata aaaaagtatt tattgccggc agcacatggc ctgccgatga
    15961 tgatattatt tgcaaactta taaacgattg taccgatgat tataaattta taattgctcc
    16021 acatgaaata agtgaaacta aactgagtga gttattacaa aagataaaaa aatctgctgt
    16081 gcggtattct cagtttacca tcgacaataa agagtgcgaa gtaatgataa tagataatat
    16141 tggtttactg tcaactttgt atcgctatgg cacaatcact tatgttggtg gcggctttgg
    16201 cacaggcata cacaacatac tggaacctgc agtgtatggt ctgccggtaa tagtagcacc
    16261 gcaatactca aaattcaacg aagctttggc attactaaaa ataagaggaa tgtttgttgt
    16321 gaaaaatcat caaaccctca aaacggtttt tgatgatttg aaatataact tcgaaaagtt
    16381 gcaggcgata aaaacagcaa atttgaaata catcgatcaa caaaaaggtg caaccgatat
    16441 tattataaat tatctgaaat tgaattggga ggaatagggt tgttagttta aagtttttag
    16501 tttaaagttt gaagttgaga gtttaaagag gagagtttaa agtttgaagt tgagagttta
    16561 aagtttaaag tttttttgca gcatttagac attccgatta ctatcattac aagaaatatg
    16621 gtgttgtttg gattgcaatg aatagtattt ttatacagca cattttaaaa atcacaataa
    16681 gcatttacaa cctaaaaact taacaaccca ataactcaac aaccaataac tgaacaaccc
    16741 aacaactaat aacccaataa cattataacc gcttcctcga ggtaagttgc tctatacgct
    16801 tctcataatt agctccttga aaaatgcgat gcacataaat tcccggcaca tgtatttcat
    16861 tgggatcgag ttcaccaggt tgcacaagat gttctacttc ggcaattgta atttttccgg
    16921 ccatagccat caatggatta aaattgcgcg ctgtagcacg aaaaattaaa ttgccggtgg
    16981 tgtcaccttt ccatgctttt actattgcaa aatcagcatc gaatgcttcc tccatcaagt
    17041 aatcgcggcc tttccagttt ataatttgtt tgccctcggc aacttcggta ccaacgccag
    17101 caggtgtggg aaaaaacgga atgccatatc cactcatcat gcaacgcgta gcaagtgtgc
    17161 cttgaggtat taaatctact tgcaattcgc cactcaacat ctgtcgctcg aactcggcat
    17221 tttcgcctac atagctcgat atcatttttt tcacttgctt attttgcagc aacaaaccca
    17281 agccaaaatc gtcaattccg gcattgttcg aaacgcatgt taaattattg atgcccattg
    17341 caaccattgc gttaatgcaa ttttcgggta tgccacacaa cccaaaccct cctactaaaa
    17401 gtgtcattcc actttttata tctgctgtgg ctgtggctgc gtcttttact gttttgatta
    17461 tcataatata aatgcttaat gtgtttttat aaaaattgct gtgcagcaaa gttgttacat
    17521 tttattgatt actcaaagct tgaggcaaaa gcaattaata tttttcgcaa aaatgttttg
    17581 ggttgcaaat ctatatcata aaatatattc gccttcaaaa ttattgttat atttgtcaaa
    17641 gggttttaaa ttaaaaatct atttagcatg aaacagtttt tagcaaatct attttattta
    17701 tttttatcgt gcacattgat tatttcgtgc ggaggtaaat ttgaatttga aaagcaaggc
    17761 cgcaagccag tctatgtgag ctacgataat ctttttcaat acagccaaac agtgccacaa
    17821 cctatacaga acagtggaaa aatattgctt tatggcaatt acctttttat gatagaagta
    17881 aacaaaggcg tgcatgtaat tgacattagc gacactgcca atccacaaaa aatgtcgttt
    17941 ctcaatatcc ccggcaataa ggacattact tcgcaaaata attttttgta tgccgataat
    18001 ggtcccgatt tattggtgct tgatttgagc gatataaaca atataaaatt gataacccgg
    18061 caaaaaagtg tttttaagcc cagcgagttt tatccttcgg gtttcatagg ctttttcgaa
    18121 tgtgccgatt atgcaaaagg atgggtgatt gcctgggaaa cggcattgct gcaaaatcct
    18181 gaatgcaggc gcgaataaat gttaaaaatg tttgcaatta taaattgttt ttattccttt
    18241 actaaaaata aataatcatc ggtatgaaaa acattctcaa agcagttctt atttgcagta
    18301 ttattttttc tgcatgtaca aaagaaaacc aatcagttac tgctgatcgt actgcacagg
    18361 gcacaaacgg atcgcttacg cgttttgcta taaaagataa ttttatgtat gcgctcgatg
    18421 tcaactacat gaaagtattt gacatttcta cgggcagcga tcctatatta gttaactcgg
    18481 ttaagattaa ctatggttta gaaactatta ccatatacgg agaatatgta tacataggtg
    18541 cacacgatag aatttacatg gtaggcatta atgatccttc gcaaccactg gagtacgatg
    18601 aaatgcagca caacattagt tgcgacccgg tagtagtgca aggcaattat gcatactcca
    18661 cacaaaatag ttttgaagta ggttgtggtc atgcaacatc gcaaagtatt ttggcggtgt
    18721 acaatatttc taatccacaa caaaccaatt tactaaaaag cattacaatg acttcaccat
    18781 atggattggc agttgaaggc aaaaatcttt atgtgtgcga tagcgaagaa ggagttattg
    18841 tttacaatat atcaaatccc gaatcgccac aagcactaag taccattcct gttgcaaatt
    18901 cgcgcgatat aattttgaat tttccttaca tgataatttc tactcaaaca agttttgaaa
    18961 tgtataatta tcaggatgta aacaacatat accatgtaag cacatttcaa ctaaagtaaa
    19021 tgcgccaagc gttttttgtt gtaattcttc ttttttgttt tcacaaatta tttagtcagg
    19081 atacaccttc gtattctaag ttcagtttag ttactcgccc tttggcgtgg gtcgatcctg
    19141 tcaatcaaag tttttctgta ggccttcaat atcgtgtatc gcaaaagtgc attgtcgaag
    19201 tgcaacccgg tattattcat tccgcttatc aaaatttaat tgtcgattcc tttaatgttc
    19261 attatgcagg gtttcgtggt tcggccgagg cgatgttttt tattgaagaa agtccttatt
    19321 atgtttcgtg cgagtatttg tataaaaatt ttacacgcag taaagatgaa tggatgttgc
    19381 gcatgggctc aacctatcag caaaaatttc gggtcgataa atattatcgt gttagttctt
    19441 acaggttcaa agccggtgtt accaccaata gaaatggaag cagagtacat ttagatttag
    19501 cagcaggcat aggccttaga acaaaaaatg tttccttttc gcctttgccc gaagatgccg
    19561 aatttcttga gcaaagtaat ttcgaaaatg aaactgttgc cggccgttcg tttgtgcccg
    19621 aattattttt tacaataagc atggtttata a
//
LOCUS       DAORQA010000026        19286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_726_length_19286_cov_13.823462, whole genome shotgun sequence.
ACCESSION   DAORQA010000026 DAORQA010000000
VERSION     DAORQA010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 19286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19286
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_726_length_19286_cov_13.823462"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PK736_03440"
     CDS             <1..299
                     /locus_tag="PK736_03440"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HRC32479.1"
                     /translation="STTESDDYFATRPRESQIGAWASAQSENLESRDMLLNKITELEK
                     KYTDMPVPRPPHWGGFRVVPNYFEFWQGRESRLHDRLAFTLTDDETWTRQVLQP"
     gene            425..1684
                     /locus_tag="PK736_03445"
     CDS             425..1684
                     /locus_tag="PK736_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRC32480.1"
                     /translation="MFLRMLVTVVFLFVIDIYVFSGLKVLLRNSTLISERTGTIIFWS
                     LCGLCYALILAAAIYDYHEWNKTFRTYSFAFVVIFYFSKLFFVVFLLIDDIIRGVRYI
                     IGKFGKSDANEVSLASGGEKISRQSFIVKTGLLVAAIPFGAMIYGMVKGAYNYSVRKI
                     TLKHDGIPKAFDGFRIVQISDLHTGSFTFEHKVDRAVDIILAQKPDIVFFTGDLVNEK
                     SIEAEPFIELFGRIKAPHGVFSILGNHDYGDYHRWDSPEAKIANLDRLKAIHAEMGWN
                     LMLDTNHHVEKDGEKIGLVGVQNWSSHMQFPKYGDLGKATNNFVPQGFNILLSHDPSH
                     WTSEVLTKFPFIHLMLAGHTHGMQFGIEIPGFKWSPVQYIYKEWAGLYTAAKQHLYVN
                     RGLGFLGYPGRVGIMPEITVIDLKYSS"
     gene            2082..3647
                     /locus_tag="PK736_03450"
     CDS             2082..3647
                     /locus_tag="PK736_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HRC32481.1"
                     /translation="MKGAFDFYITNYFKSAQFFAKLIFSILLCTTTIGVYAQEKKEKI
                     KLEHADSLVFDDVVSIARRLKGNVVFSQGEVKLYCDSAYFYDATNVVDAYENVRIVKN
                     NDLNLTGKFLHYDGDSKLATITNNVRMTDNKMVLTTEALDYNMNTETGFYNNGATIVD
                     KDNTLTSQFGYYFSNTKMLSFKKNVVLVNPRYRLTGDTLMYNTATHTATFVGPTYIKS
                     SGRDATFIYCESGWYNTDTDKSFFRKNSFIQSKEQKLFGDSLRYDKKAGTGEAFGNVM
                     ISDSLQKTIIAGDYGKYNEISGQTFVTGRSQMIQIFDSDSLFMHADTLFAKSDTSGKQ
                     KTYYAYHHVKFFKKDFQGKCDSLTYSTTDTLMRMFALPVLWSDSNQITGDSIHLQMEN
                     NKIARMYMYESAFIASYEDTNRYNQIRGKNMIGYFENNELHKINVEGNGQTIYYGRNN
                     AKQLVGVNRADCSDLVIHLDSSKVSKIILLNKPEATFYPIDELNTSELLLRGFKWRNK
                     ERPMKKEDIFSIE"
     gene            4045..4560
                     /locus_tag="PK736_03455"
     CDS             4045..4560
                     /locus_tag="PK736_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRC32482.1"
                     /translation="MKKSRKPESFIQKTVYPGGVDALRKDIMELMNYPEEAIKANVEG
                     SIVVTMDIDNKGIVSNAKIKKGLGHGLDEEAVRIATALKFTSTHNRGVRVVFHETLNI
                     SFNIREYLKYKQEKEAAKMIQIKTNNPGLQIQNIQISYNTPEPKNVAPEKKEEVKYNY
                     TIATNNNPEGN"
     gene            complement(5154..7475)
                     /locus_tag="PK736_03460"
     CDS             complement(5154..7475)
                     /locus_tag="PK736_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRC32483.1"
                     /translation="MEIRKTAWQIIFVMCFTLFCMQCNVGLNAQPNQYNIIPYPAQLD
                     TLQGHFTISDNTILCSQNTTAEIKSCWAFFNSYINAIGGTSLKVGERSDYASDCVVYF
                     LDNSIKHNEGYSINIMPDKVEIYSKTNAGLFYALQTLRQLMGSKTQKVTTTPITIPCC
                     KITDSPRFEYRGMHLDVARHMFPLEFVKKYIDLMAFYKYNNFHWHLTDDQGWRAEIKK
                     YPLLTQVGGMRKETTTNISRDKEPVTIKESYGGFYTQSQMKEVVEYATARHINVIPEI
                     EMPGHSLAALSAYPYLGCENKPIEVGTRWGVFEDVFCPRDTTFSFLQDVLTEIMQIFP
                     SQYIHIGGDECPKEAWKKSAFCQQLMKRENLKDEMELQSYFVRRIEKFLNANGRQLIG
                     WDEILEGGISPNATIMSWRGTAGGIEAARQKHDVIMTPGSHCYFDHYQYVRASEPYAI
                     GGFSPVEKVYSFEPIPSELNAEEGKFILGAQANLWTEYIDTSTYAEYMAYPRACALAE
                     VLWTPKDKRNYDNFLNRLADNFLHLDAMDVNYAKHVLGVYGKMVRNDDGGLYYELDSK
                     NRDAQIKFSINDRDLKTTKDYKAPIFINQSCAISAATFAGNKRLSDVYTEQITYHNGA
                     GANVLMMSPWHENYNPGSSAAIVNAIQGSNNYGDGQWFGYSGNDCEVIIDLQKVQSIN
                     TLKTNFIEKKNSWIYAPSQVQFFVGNDTAKMEMVFETKPTGGQVINTIVTPALNTSAR
                     YIKMIMKNYGIIPSGNDGAGNKAWMFVDEILVD"
     gene            7662..8519
                     /locus_tag="PK736_03465"
     CDS             7662..8519
                     /locus_tag="PK736_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32484.1"
                     /translation="MSYIKCNHCNQFTELSSEYLTFCSHCGKKLQNNFNDWKHQNAGK
                     TFDDFKNTFGIDNIENALPKKVRNYNPAAMYFFVGLGILLFSSFCYLTVISIQSLLLL
                     TNISNAISHNATTTGATAKNDDWTTQSLGKMGYTILTPYQFSELQIPLPPEVQTMMNG
                     LECYQAIDKEGMMVFVANMETADAIYLDENTMTEFMNRFSSIFINSDVGTLEKKSFMQ
                     SKKEGMLLHGDVEIGQSKSKLIVHTLFENHKAWSSFYIIPQGRKDLEIVAHRMFQNVE
                     INDGDGAVQ"
     gene            8568..8957
                     /locus_tag="PK736_03470"
     CDS             8568..8957
                     /locus_tag="PK736_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX-like family protein"
                     /protein_id="HRC32485.1"
                     /translation="MTNRKIYKIISYCIATVWIANGLFCKVLNLVPRHEQIVKRILHV
                     DLITANVFTILIGLSEIIMAVWILSGYKSKLNAIAQIAIVATMNTLEFILVPDLLLWG
                     KVNSIFAFIFILVVYFNEFYLNQKQHN"
     gene            8962..9702
                     /locus_tag="PK736_03475"
     CDS             8962..9702
                     /locus_tag="PK736_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HRC32486.1"
                     /translation="MLSFLKNHPFAVEAYFESSLVFTFAVPKEQLQNLIPECLQLDTF
                     QDKWAFIAVAMVQTKDLRPKGFPKFMGNDFFLIGYRVFVRYINNAGKNLRGLYILKSE
                     TDKTKMEHLGNIFTHYNYTTTDVKQIVQQNTKEIFSIKSKFKITIDTIEEEISLPEQS
                     PFADWKEARRFAGPLPFTFTYNKETKEVLIIEGVRQNWTPNPVKVIDYYFDYLYSLKI
                     DKPVLANAFIIKNIPYYWEKGTMEVWSK"
     gene            9690..10448
                     /locus_tag="PK736_03480"
     CDS             9690..10448
                     /locus_tag="PK736_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRC32487.1"
                     /translation="MEQVRKPFQGVLNIILFNWPFYVSAFFLFLITFYTANYFEKDYA
                     NFIQIISILALASIIVSLVVSLYVYDISNLYRLNWIDKSNDERLIVNINAGFDETSYL
                     LQDKFKNSDLIVLDFYNPILHTEASIKRARKAYPSFPKTKHIKTSDTELTDNSADKIF
                     VILSAHEIRNENERAEFFKELYRVIKPKGQIYVTEHLRDLPNFFAYNIGFFHFHAKSS
                     WLKTFKDANLKIKQEIKLTPFISTFILYKNGNTI"
     gene            10432..10827
                     /locus_tag="PK736_03485"
     CDS             10432..10827
                     /locus_tag="PK736_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32488.1"
                     /translation="MEIQFKIIGVLLIALALIHIVFPKYFKWNKELKSLSLINRQMMK
                     VHTFFIALTIFLMGLLCLTSSSELIETSLGKKVSLGLGIFWTFRLFIQLFGYSTDLWK
                     RKTFETFIHIIFSLLWAYLSIIFLKTYFN"
     gene            10964..13156
                     /locus_tag="PK736_03490"
     CDS             10964..13156
                     /locus_tag="PK736_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRC32489.1"
                     /translation="MACLKNISKNFVSTKINFVVIIFLLVVIIPQKSIAQQPYNILIL
                     AIDDLNDWVGFLDGNQQSITPNMNKLASQSVNFKNAHCAESVCNPSRVSILTGFLPST
                     SKVYTNQDTMRQFPVLQNCITLPQYLIQKGYKTMRYGKVFHIQDPQSWSQNGYLNGGS
                     WGTLPAVPGFTPNGIPVNDVDEYFYWGSIPDSLFKTTPDYLCTKWSATQLNSTQTKPF
                     LLACGFTRPHDPWQVPKMFFDRFPINSIVLPNIDTNDLLDIDTSMTPGVEYIAVKNYG
                     LQKEVVQSYLAAVSFADSCVGLVLDALSKSAYANNTIVVLFGDNGFHLGEKLWYDKKT
                     LWEESTRVPLLIKVPGVAPSSVTTPVSLCDIYPTLLDLCNLPLNTNNEGESLKPLILN
                     PLLSNTRVALTSSINTEVYSISGQTVRSKNWRYSVHESGALELYNHNNDPMEFINLAS
                     NITYDSTRNYMATQLNKALFKRNSTRLAVRTNYIPGIIQAEKYDMGGEGIAYHDEDTI
                     NFGGYYRMKDGVDIAPCLDSAGGLQIDSIKYGEWLSYTIKQIDSGGYNLKFRVLTNST
                     TTKTIKVFVNNVLAVQTNIPNTNNVWQTISSPVFNLQKKTNARLKVTFNGKDFKLNSF
                     EFVKKPSVARSEAKVYMTENFLDVKPNPVSGESQIYFTMAEDGIANLYITNTSGEKVC
                     TILNEYMSKGIKEVNFVPSQYNLPAGIYICTIKANNQQDCSKKIIISK"
     gene            13308..13784
                     /locus_tag="PK736_03495"
     CDS             13308..13784
                     /locus_tag="PK736_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC32490.1"
                     /translation="MNEIEILNYSEENASYIKALNVEWLEKYFAVEPNDELQLSNPRT
                     EIIDKGGMIFYASYNNEIVGTATLLKVSDNVFELSKMAVTERAQGLGIGKKLMNHCLK
                     IATQNNIQTLILFSNTKLEKAIEMYRKYGFVEVPINLNHYKRANIKMELKMEKGRF"
     gene            complement(13912..14484)
                     /locus_tag="PK736_03500"
     CDS             complement(13912..14484)
                     /locus_tag="PK736_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32491.1"
                     /translation="MQNIEPYYNWRDLYIASDDSLSPFYNVVYSEFEYTNTIYNYYIH
                     PQWDDIGSPTLYIKIIFVDYEIGFAIIELIGEWNDAINNDIMFLKRDIADVLIQNGIN
                     KFLLIGENVLNFHGSDDCYYQEWYEDISDNGGWIAAINFRHHVTQEFRKNGIDYYFVY
                     GGDFDDFNWRLLKPNQLFDLVQDMIQHRIG"
     gene            complement(14491..15312)
                     /gene="murQ"
                     /locus_tag="PK736_03505"
     CDS             complement(14491..15312)
                     /gene="murQ"
                     /locus_tag="PK736_03505"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRC32492.1"
                     /translation="MGNTVTESASNYQHLEKLSVSELLQGINAEDKKVALAVELCIPK
                     IVPLVESIITKMKIGGRLFYIGAGTSGRLGIVDASECPPTYGIEHGRVVGIIAGGDVA
                     IRKAVEFAEDDVEQGWKDLAAYTLTVNDTVIGIAASGSTPYVVGALQQCRANGITTGC
                     ITCNTDTNVAKHSDFAIECLVGPEFVTGSTRMKAGTAQKLLLNMISTSVMIGLGHVKG
                     NKMVDMQLSNNKLIARGTMMIMDELKIDETLAADLLQQHGSVRKAVDAYIMMRTK"
     gene            complement(15321..17744)
                     /locus_tag="PK736_03510"
     CDS             complement(15321..17744)
                     /locus_tag="PK736_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRC32493.1"
                     /translation="MKEAKQEANTLMSFEQFKLSVVDDYRIANISRQASLLGRREVLT
                     GKAKFGIFGDGKEVAQIAMAKVFKHGDFRSGYYRDQTFMFAAGLLTVQQYFAQLYADT
                     DTDADPCSQGRLMNGHFATKLLDEKGDWLNLTQLKNSSADISPTAGQMPRLLGLAYAS
                     KLYRNNNDLNNVDFSKFSINGNEVAFGTIGDASTSEGLFWETMNAAGVLQVPLAMSVW
                     DDGYGISVSKKYQTIKQSISEALSGFATTGDKDGFLIYTAKGWDYPTLCSVYKEGINF
                     CRKNHMPVLFHITDVTQPQGHSTSGSHERYKSKERLDWENDHDCIAKMREFILENGIS
                     TEDVLQQIEKEAKQHVNESKKRAWDEFCTSINNDRISAVAVLNKVKENTSQKVFVEKI
                     IADLNDTNELDRKAIITAVKKCLRLTINDKSDERKSLVQWMQAFEQNMHNRYESHLYS
                     ETENNALKIKSIAPVYSDNAVMMDGREILQANFDALLNMYPEMLAFGEDVGKIGGVNQ
                     TYAGLQQKYGELRVQDTGIREATIIGQGIGLALRGLRPIAEIQYLDYLAYAINVLTDD
                     LACLSYRTKGFQKAPLIVSSRGHRLEGVWHSGSPMQFILGALRGMYILVPRDMTTAAG
                     FYNTLMKSDNPALIIECLNGYRLKEKMPSNLGEFCLPLGIPEVLMDGDDVTIVTYGSC
                     IRVAMDSQKQLSEMGISVELIDVQTLIPFDINHLICKSLQKTNRIIFVDEDVEGGATA
                     YMMQQVLEVQNGYQFLDASPLTVSANDHRPAYGSDGDYFSKSNAEKITEAVYSLMHQS
                     NPAQYPKLY"
     gene            18049..>19286
                     /locus_tag="PK736_03515"
     CDS             18049..>19286
                     /locus_tag="PK736_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HRC32494.1"
                     /translation="MKHNLLLILLITFVTPALWSQCTNTNATSCQCANQGTTNCDLLP
                     DIIPAKTLLLDTGAYGIIEYTYNDPTINRGRLRVSVSTPNIGHGPLEIQTDSTFVCGT
                     DTITGIPPATCTNTGLPPKILVRQYIYHKNGNVMTKIKKPAGSMTYHPTHGHMHVDNW
                     GTYTLRTSTSNPDPLTWPIVATGQKLAFCLMDFGTCQTFIGHCVDSNNTVLGNANFPN
                     LGLGGAAYTCSNVKQGISSGYTDIYYQYLDGMYIQLDTNICNGDYWIVVQLDPLNYFT
                     EENENNNVIAVPYTLKNQRPLPVINLTGNQNICLGNSVNLTAAGATTYTWSPASGLNK
                     TTGTIVTASPTSTTTYTVIGSKIGAGCIDTNYVTVNLVTTANISVTGNNGICTGSSTT
                     LTASGVNTYSWAPATGLNAT"
BASE COUNT         6132 a         3663 c         3509 g         5982 t
ORIGIN      
        1 taagcaccac cgaaagcgat gattattttg ctacccgccc ccgcgaaagt caaataggtg
       61 catgggcatc ggcacaaagc gagaatcttg aaagccgcga tatgttgctc aataagataa
      121 cagagttgga aaaaaaatat accgatatgc cagtaccacg acctccacat tggggtgggt
      181 ttcgtgtggt gcccaactat ttcgaatttt ggcaagggcg cgaaagccgc cttcacgacc
      241 gtttggcttt tacacttacc gatgatgaaa catggaccag gcaagtatta cagccgtaat
      301 taatgcatgg atattcttgt taatttacca ttaataattc taatcgactc atgtttccat
      361 gaataatgtg aaacaaattt cccttattgt tgtctactta atataaattt taaaaaaagg
      421 ctttatgttc ttacgaatgt tggttactgt tgtttttctg tttgttatcg atatatatgt
      481 tttttctggc ttaaaagttt tgctccgcaa ttcaacttta attagcgagc gcaccggcac
      541 tattattttt tggtcgttat gcggtttgtg ctatgctctg attttggcag ctgcaattta
      601 tgattatcac gaatggaata aaacctttcg cacttactcg tttgcctttg tggtgatatt
      661 ttatttttcg aaattatttt tcgtagtgtt tttgttgatt gatgatatca ttcgtggggt
      721 gcgatacatt ataggtaagt ttggcaaaag cgatgcaaac gaagtgtcac ttgcgagtgg
      781 tggcgaaaaa ataagccgcc aatcttttat cgttaaaacg ggcttattgg ttgctgccat
      841 tccgtttggt gctatgatat atggaatggt gaaaggcgca tacaactatt cggtgcgaaa
      901 aattacattg aagcatgatg gtattcctaa ggcatttgat ggttttcgca ttgtgcaaat
      961 atcggacttg catacaggaa gttttacctt tgaacataaa gtggaccgcg ctgtggatat
     1021 tattttggca caaaaacctg atattgtttt ttttacgggc gatcttgtaa acgaaaagtc
     1081 gattgaagcc gaaccattta ttgaattgtt tggccgcata aaggcgccac atggcgtatt
     1141 ttctattttg ggaaatcatg attatggcga ctatcataga tgggatagcc ccgaagcaaa
     1201 gattgccaac ctcgacaggc ttaaagccat acatgccgaa atgggttgga acctgatgct
     1261 cgataccaat caccacgtag aaaaagatgg cgaaaaaatt ggtttggtag gcgtacaaaa
     1321 ctggagttcg catatgcagt ttccaaagta tggcgacctc ggcaaagcca caaataattt
     1381 tgtgcctcag ggtttcaaca ttttattatc gcacgaccct tctcactgga caagcgaagt
     1441 gcttacaaaa tttcctttca tccatcttat gcttgcgggc cacactcatg gtatgcaatt
     1501 cggtattgaa atacccggat ttaaatggag tcctgtgcaa tatatttaca aagaatgggc
     1561 aggtttgtat actgcagcaa agcaacacct ttatgtaaac cgtggcctgg ggtttttggg
     1621 ctatcccggt cgcgtgggaa taatgccgga gattaccgtg attgatttga aatacagttc
     1681 ttagttacgt ttgcggagag gattgttctt tgttcttagt tacttttgcg gagaggattg
     1741 ttcttcgtcc cgacactttg gtcgggatgc cgaccgaagt gtcggcattc ttcgtttcgt
     1801 ttacggagag ggatgttctt agtttctttt acggtcagga aagtttctag tttggagttt
     1861 tgttgctgac ttctttcctt cgacttttca caattcacga ttcacatttc acttttgcct
     1921 tttctcgttt gatttttcac aattcacgat tcacatttca ctttttcctt ttctcttttg
     1981 atttttcaca attcacaatt gacaattgac aattgactta tttcttttta cttttttctt
     2041 ttggcttatt tctaatgata aatgatttat tttgttccgg aatgaaagga gcatttgatt
     2101 tttacatcac aaactatttt aagtctgcgc aattttttgc aaaactaata ttcagcattt
     2161 tgctttgcac cacaaccata ggtgtatacg cacaggagaa aaaagagaaa ataaaactcg
     2221 aacatgccga ctcgctggtt tttgatgatg tggtgagtat tgcccgcagg cttaaaggca
     2281 atgtggtttt ttcgcagggc gaagtaaagt tgtattgcga tagtgcttac ttttacgatg
     2341 ccaccaatgt tgtggatgct tacgaaaatg tgcgcatagt aaaaaataac gacctcaatc
     2401 tcacaggaaa atttttgcat tacgatggcg attccaaatt ggcaaccata accaacaatg
     2461 ttcgcatgac cgataataaa atggtactca ccaccgaggc attggattat aatatgaaca
     2521 ccgaaacagg tttttataac aacggtgcaa ccatagttga taaagacaat acgctcacat
     2581 cacaatttgg ttattatttt agcaatacaa aaatgttgtc gttcaaaaaa aatgtggtgc
     2641 ttgtaaaccc tcgctaccgg cttacaggcg atacacttat gtataatacc gcaacacaca
     2701 ccgcaacttt tgttggtcct acttatataa aatcgagcgg ccgcgatgct acttttattt
     2761 attgcgaaag tggatggtac aatactgata cggataaatc atttttcaga aaaaattctt
     2821 ttatacaatc gaaagagcaa aaattatttg gtgatagttt gcgctacgat aaaaaagccg
     2881 gcacaggtga agcatttggc aatgtgatga ttagcgatag tttacagaaa actatcattg
     2941 ccggtgacta tggcaaatac aatgaaatta gcggacaaac ttttgttacc ggccgctcac
     3001 aaatgataca gattttcgat agcgattctc ttttcatgca tgccgatacc ttgtttgcaa
     3061 agagtgatac aagcggcaag caaaaaactt actatgctta tcatcatgta aaatttttta
     3121 aaaaagattt ccagggcaaa tgcgactccc tcacctactc tactaccgat acacttatgc
     3181 gcatgtttgc gttgcctgtg ttgtggagcg atagcaatca gataactggc gacagcattc
     3241 atttacaaat ggagaataat aaaattgccc gaatgtacat gtatgaatca gctttcattg
     3301 catcgtatga agacaccaat cgctacaacc aaatacgggg aaaaaacatg ataggctatt
     3361 ttgaaaataa tgaattgcat aaaataaatg tggagggcaa tggacaaaca atatattatg
     3421 gtcgtaacaa tgccaaacaa cttgtaggtg taaaccgtgc cgattgcagc gaccttgtta
     3481 tacaccttga tagcagtaag gtttcgaaaa taattttgtt gaataaaccc gaagcaactt
     3541 tttatccgat tgacgaattg aatacgagcg aacttttact acgcggtttt aaatggcgca
     3601 acaaagaaag accgatgaag aaagaggata tatttagtat tgagtagttt agtattgagt
     3661 attgggatta ttggtattgg gtattgagat tattagtatt gggtattcgg attattggta
     3721 ttgggatttt tagtattgag tattgagatt attaagattg ggattttaat attggggctg
     3781 aagttgatgg ctttgtggta ttaatagatg taaccaacgt tgaataatta tctattccat
     3841 attgttgaat ttacttttgt gtttcgattt tgttttacat ccttagatgt ggatttatca
     3901 ggcctttaat tttgcataaa agaagtaatg aatttttcca atgaacgatt ctagattaac
     3961 cttcttactt ttttttggca agttcgtgtt tcatatccaa ttaatatttc ttctttgcat
     4021 tcaacatttt ctaacaaaac aaaaatgaaa aaaagccgta agccggaaag ttttatacag
     4081 aagaccgtgt acccgggcgg tgtagatgca ttacgtaagg atatcatgga gttgatgaac
     4141 tatcccgaag aggcaataaa agcaaatgtg gaaggaagca tagtggtaac catggacatc
     4201 gataataaag gaatagtgag taatgcaaaa ataaaaaaag ggctaggcca tggcctcgat
     4261 gaagaagcgg tgcgtattgc cacagcatta aaatttacat ccactcacaa ccgcggtgtg
     4321 cgtgtagtat ttcacgaaac gttgaatatc tctttcaaca ttcgcgaata cttgaaatac
     4381 aaacaagaaa aagaagcggc aaaaatgata cagataaaaa ccaataaccc gggattgcaa
     4441 attcaaaata ttcaaataag ctataataca cctgagccaa aaaatgttgc accagaaaaa
     4501 aaggaagaag taaaatacaa ctatactatt gcaacaaata ataatccgga aggaaattga
     4561 attgaagggt tagaaaaata aataattttt caagggttta aatacttttc aataggggct
     4621 ttgcaaagga ggaaagactg tagcactttc attgtcgaaa atactttact cagcagagat
     4681 tgcttcgctt cgctcgtaat gacgtgaata atttctctct tcccgtcaat gcgaaggagg
     4741 aacgactgaa gcaatctcat tctcgaaaag accttactca gcatagtttg cttcgcagtt
     4801 cgagaatact cactgtgaca aatcgcaatg gcgaacaaag aacaatcctc accgcaaaca
     4861 aaactaagaa tgccgacact tcggtcggca tcccgaccaa agtgtcggga caaagaacaa
     4921 tcctcagcgc aaacgttaca aagaacaagg ctttcagcag acgtatctaa gaacgaagaa
     4981 caatcctctc cgcaaacgaa actaagaatg ccgacacttc ggtcggcatc ccgaccaaag
     5041 tgtcgggaca aagaacaata ctcaccgcaa acgaaactaa aaactaagaa ctataaatgc
     5101 cgacaccttg gtcggcatcc cgaccgtagt gtcgggacta aaaaccgaat taactaatcc
     5161 acaagaatct catctacaaa catccaggct ttattgccag caccatcgtt tcccgaagga
     5221 atgattccat aatttttcat tatcattttt atgtaacggg cacttgtatt cagggccgga
     5281 gtaacaatgg tattaatcac ttgtccgcct gtgggtttag tttcaaatac catttccatt
     5341 ttcgcagtat cgttgccgac aaaaaactgc acttgcgaag gtgcgtatat ccacgaattt
     5401 ttcttctcaa taaaatttgt tttcagtgta ttgatgcttt gtactttttg caaatcgata
     5461 atcacttcgc aatcgttgcc cgagtaacca aaccactgtc catcgccata attattagat
     5521 ccttgtattg catttacaat tgcagccgat gaaccgggat tataattttc gtgccatggt
     5581 gacatcatca aaacatttgc ccctgcccca ttgtgatagg ttatttgctc ggtgtaaaca
     5641 tcgctcaatc gcttgttgcc ggcaaaagtt gctgccgaaa tagcacacga ctgattgatg
     5701 aaaattggag ctttataatc tttagtagtt ttgaggtcac ggtcattaat tgaaaacttt
     5761 atttgtgcat ctctattctt cgaatccaac tcgtaataca atccaccgtc atcgttgcgc
     5821 accatttttc catacacgcc cagtacatgc tttgcataat ttacatccat ggcatcgagg
     5881 tgtaaaaaat tatccgccaa tcgatttaaa aaattatcgt agttgcgctt gtcttttggt
     5941 gtccacaata cttctgccaa tgcacatgca cgagggtatg ccatgtactc cgcataagtg
     6001 gaagtgtcaa tatattcagt ccataaattt gcctgggcac ccaaaataaa tttaccttct
     6061 tctgcattta attccgatgg aatgggttcg aaagaataca ccttctctac aggcgaaaac
     6121 ccgccaatgg catacggttc gcttgcacgc acatactgat aatgatcgaa ataacaatgt
     6181 gagccgggtg tcattatcac atcgtgtttt tggcgtgcag cttctatacc tccggcagta
     6241 ccgcgccaac tcattatggt ggcatttggc gaaataccgc cttcaagaat ttcgtcccat
     6301 cctataagtt gccttccatt tgcattcaaa aacttttcta ttcgtcttac aaaataactt
     6361 tgcaattcca tttcatcttt caaattttcg cgcttcataa gttgctggca gaaggccgat
     6421 ttcttccatg cttctttggg gcactcatca ccaccaatat gaatgtattg cgaaggaaat
     6481 atttgcataa tttcggtaag cacatcctgc aaaaaactaa acgttgtatc gcgtgggcaa
     6541 aacacatctt cgaaaacgcc ccaacgtgtt cctacttcta tcggtttatt ttcgcatccc
     6601 aaatatggat aagcagacaa ggctgcaagt gaatgtccgg gcatttctat ttcgggaata
     6661 acattgatat ggcgcgctgt tgcatattcc actacttctt tcatttggct ttgtgtataa
     6721 aaaccaccat acgattcctt aatggtaaca ggttctttat cgcgcgaaat attagttgtg
     6781 gtttctttgc gcattccgcc aacctgagtt agtaatggat atttttttat ttcggcccgc
     6841 caaccttgat catcggtaag gtgccaatga aaattattgt acttataaaa tgccatgagg
     6901 tcgatatact tttttacaaa ctctaaagga aacatgtggc gggcaacatc gaggtgcata
     6961 cccctgtatt cgaaccttgg agaatcggtg attttacaac atggtattgt aatgggagtt
     7021 gtagttactt tctgcgtttt acttcccata agttgccgca aggtttgcaa agcataaaat
     7081 aatcccgcat tggttttact ataaatctct accttatcgg gcataatatt tatactgtaa
     7141 ccttcattgt gctttataga attatccaaa aaataaacaa cacaatcgct ggcatagtcg
     7201 ctgcgctcgc ctaccttgag cgatgtacca cctatagcat tgatatatga attaaaaaaa
     7261 gcccaacaac tttttatttc ggcagtagtg ttttgcgagc acaatattgt attgtcactg
     7321 atagtaaaat ggccttgcag agtgtcgagt tgtgccggat acggaattat attgtattga
     7381 tttggctgcg cattcaatcc aacattgcat tgcatacaaa acaaggtaaa acacattaca
     7441 aaaataattt gccaggccgt ctttctgatt tccatattta aatttatgat tttagttttg
     7501 aaaaaatatc tatttggcgt tttattaatt tcacataatt gcatttgcaa aaacaatgat
     7561 aaaattatag ctgccgaaat tatcaaagtt aatttacatt tggcaaaata ttatttcaaa
     7621 tatctttaat aataaaatag aaaaacagta aaccgaaaaa catgagctat atcaaatgca
     7681 atcactgtaa tcaatttacc gaactaagta gcgagtatct tacattttgc agccattgtg
     7741 gaaaaaagtt gcagaataat tttaatgact ggaaacatca aaatgccggc aaaacatttg
     7801 acgattttaa aaacactttt ggcatagata atattgaaaa tgcactaccc aaaaaagtaa
     7861 gaaattacaa ccctgcagcc atgtattttt ttgtgggact gggtattctt cttttttcta
     7921 gtttttgcta tctcactgta atttccattc aaagcttgct gttacttaca aatattagca
     7981 atgctatttc acataacgca actacaaccg gagccactgc taaaaacgat gattggacaa
     8041 ctcaaagctt aggcaaaatg ggatacacca tacttacacc ttaccagttt agcgaattac
     8101 aaataccact tcctcccgaa gtacaaacca tgatgaatgg acttgaatgt tatcaggcaa
     8161 tagataaaga aggcatgatg gtttttgttg ccaacatgga aacagcagac gccatttatc
     8221 ttgacgaaaa tacgatgaca gaatttatga atcggtttag cagtattttt atcaatagcg
     8281 atgtgggaac attggagaaa aaatctttta tgcaaagtaa aaaagaagga atgctgctgc
     8341 atggcgatgt agagattggc caatctaaaa gcaaacttat tgttcacacg ttattcgaaa
     8401 atcacaaagc atggtcgtca ttttatatta ttcctcaagg cagaaaagac ctcgaaatag
     8461 ttgcacaccg catgtttcaa aatgtggaga taaatgacgg tgatggtgcg gtgcaataat
     8521 gcgaagcaaa ttatttcctg cgtgcatagc ggacaacaca accaacaatg acaaacagaa
     8581 agatttacaa aataatatct tattgcattg cgactgtttg gattgcaaac gggctttttt
     8641 gtaaggtgct aaatcttgtt ccaaggcacg aacaaattgt gaaacgtatt ttacacgttg
     8701 accttatcac agctaacgtt ttcacaatct taatcggact atcagaaatt attatggctg
     8761 tttggatttt aagtggttat aagtcaaagt taaatgcaat agcacaaatt gcaatagtcg
     8821 caacaatgaa tacgcttgaa tttatacttg ttccggactt gttactttgg ggaaaagtaa
     8881 attcaatttt tgcatttata tttattttgg ttgtttactt taatgaattt tatcttaacc
     8941 agaaacaaca caactaagtc aatgctgtct tttttaaaaa atcatccttt cgcagttgaa
     9001 gcatattttg aaagttcgct cgtatttact tttgctgtac caaaagaaca gttacaaaat
     9061 cttattccgg aatgtttaca gcttgacacc tttcaagata aatgggcttt tatagcagtt
     9121 gcaatggttc aaacaaaaga cttgcgtcca aaagggtttc cgaaatttat gggcaacgac
     9181 ttttttttaa ttggctatcg cgtttttgtt cgctacataa ataatgcagg taaaaatttg
     9241 cgcggtttat atattctgaa atcagaaaca gacaaaacga aaatggaaca tttgggaaac
     9301 atatttacgc attataatta tacaacaaca gacgtaaagc aaattgtgca acaaaatacg
     9361 aaagaaatat tctcgattaa atcaaagttc aaaatcacaa ttgacactat agaagaggaa
     9421 atttctttac ctgaacaatc gccttttgca gactggaaag aagcaagaag atttgctgga
     9481 ccattgcctt ttacttttac ttataacaaa gagaccaaag aagttttaat cattgaagga
     9541 gtaagacaaa actggacacc aaatccagta aaagtgattg attattattt cgattatttg
     9601 tattctttaa aaattgacaa accagtttta gcgaatgctt ttatcattaa aaacattcca
     9661 tattattggg aaaaaggcac aatggaagta tggagcaagt aaggaaaccg tttcaaggag
     9721 tattaaatat cattctcttt aactggccct tttatgtcag cgccttcttt ttatttttga
     9781 ttacgtttta tactgccaac tattttgaaa aagattatgc gaatttcatt caaattataa
     9841 gcattttagc attagcctca ataattgttt ctttagtggt ttccctatac gtttatgata
     9901 tttcgaatct gtataggtta aactggattg acaaaagcaa cgatgaacga ttaattgtaa
     9961 atattaatgc aggttttgac gagacaagtt atttgcttca agacaaattc aaaaattcag
    10021 accttattgt gttagatttt tataatccca tattgcatac tgaagcatcc attaaacgag
    10081 caagaaaagc atatccatca tttcctaaaa caaagcatat aaaaacatcc gatacagaac
    10141 tgaccgataa ttcagcagac aaaatatttg tgattttatc ggctcacgaa atacgaaacg
    10201 aaaatgaaag agcagaattc tttaaagagc tatacagagt aattaaacca aaagggcaaa
    10261 tctatgtaac agaacattta agagatcttc caaatttttt tgcttataat atcggcttct
    10321 ttcattttca cgctaaatct tcgtggctga aaacattcaa agatgcaaat ctaaaaatca
    10381 aacaagaaat aaaacttact ccatttattt cgacttttat actttacaaa aatggaaata
    10441 caatttaaaa taattggtgt tctattaatt gccttggcat tgattcacat agtttttcca
    10501 aagtacttta aatggaacaa ggaactaaaa tctttgagtt taatcaatcg tcaaatgatg
    10561 aaggtgcata catttttcat tgcattgaca atatttctta tgggcttgct ttgtctgaca
    10621 tcgtcaagcg aattaattga aaccagcctt ggaaaaaaag tatcgcttgg gcttggtata
    10681 ttctggacgt tcaggctttt cattcaattg tttggttact cgactgattt gtggaaacgc
    10741 aaaacatttg aaacattcat acacataata ttttcgctac tttgggcata tctaagcata
    10801 atatttttga aaacatattt caattaactt aagcaaaaac aggacgaaca ttccatctct
    10861 aacattgatt tagtgatgta ttttaattca gtatttatta tagctttgac gttatctcta
    10921 aaccatttct tttatcttta attcaaaata aaattatcac actatggctt gtttaaaaaa
    10981 tatttcgaaa aattttgttt caacaaaaat aaacttcgtg gtaattattt ttttgttagt
    11041 tgtaatcata ccgcaaaaat cgattgcaca acagccatat aatattttaa ttctggccat
    11101 agatgattta aatgactggg tgggttttct cgatggcaac caacaaagta ttacacccaa
    11161 catgaataaa cttgcaagcc aaagtgtaaa ctttaaaaat gcccactgtg ccgaatcggt
    11221 ttgcaatcca tcgcgtgttt ctatactcac aggtttttta ccatcgacaa gtaaagtgta
    11281 taccaaccag gatactatga ggcagtttcc tgttttgcaa aattgcataa cactgcccca
    11341 atacttaatt caaaagggtt ataaaaccat gcgatacggt aaagtatttc acatacagga
    11401 tccacaatcg tggtcgcaaa acggatatct taacggaggt tcgtggggga cgttgcctgc
    11461 tgtaccgggg tttacaccaa atggaattcc tgtaaatgat gttgatgaat atttttattg
    11521 gggttcaatt cccgattcat tatttaaaac aactcccgat tatttatgta cgaaatggtc
    11581 ggcaacacaa cttaattcaa cacaaacaaa accattttta cttgcatgtg gttttactcg
    11641 ccctcacgat ccatggcaag taccaaaaat gtttttcgat cggtttccaa taaattctat
    11701 tgtgcttcca aatattgata caaacgattt gctagatatt gataccagca tgacgcccgg
    11761 tgttgaatat attgcagtaa aaaattatgg actacaaaaa gaagttgtac aaagttactt
    11821 agcagccgtt tcgtttgctg actcatgtgt gggtcttgtg ctcgatgcat taagtaaaag
    11881 tgcttatgca aacaacacca ttgttgtact ttttggtgac aatggatttc atcttggcga
    11941 aaaattatgg tacgataaaa aaacattgtg ggaagaatcg acacgtgttc cgctattaat
    12001 taaagtaccg ggtgtggccc catcttcagt tacaactccg gtaagcttat gcgatattta
    12061 tccaacattg ctcgatttat gtaacctgcc gttaaataca aacaacgaag gcgaaagttt
    12121 gaaaccatta atattaaatc cactattatc taacacacgg gtagccttaa catcgagcat
    12181 aaacaccgaa gtatattcaa taagtggtca aactgtaagg agtaaaaact ggcgatacag
    12241 cgtgcacgaa agtggcgccc ttgaattgta caaccacaat aatgacccaa tggaatttat
    12301 aaatcttgca tcgaacatta cttacgatag cacgcgaaat tatatggcaa cacaattgaa
    12361 taaagcattg ttcaaaagga atagcacacg tttggcagtt cgcacaaatt atattcccgg
    12421 aataatacaa gccgaaaagt acgatatggg cggagaagga attgcctatc atgacgaaga
    12481 caccatcaac tttggcggct attatcgcat gaaagatgga gttgatatag caccatgctt
    12541 agacagtgca ggtgggctgc aaatagattc gataaaatac ggtgaatggc tatcttatac
    12601 aattaaacaa atagacagtg gcggttacaa tttaaagttt agagtgttga cgaatagcac
    12661 tacgacaaaa acaattaaag tatttgtcaa taatgtctta gcggtgcaaa caaatatacc
    12721 taacacaaac aatgtttggc aaacaataag ttcacctgta ttcaatcttc aaaagaaaac
    12781 aaatgcgagg ttgaaagtaa ctttcaatgg caaagatttt aaattgaact catttgagtt
    12841 tgtaaaaaaa ccctctgttg ccaggagcga agcaaaggtt tatatgactg aaaattttct
    12901 ggatgtaaaa ccgaatcctg taagtggaga atcgcaaata tattttacca tggccgaaga
    12961 tggtatagca aatctttata tcaccaatac atcgggcgaa aaagtttgca ctatattaaa
    13021 cgaatatatg tcaaaaggaa taaaggaagt aaattttgta ccatcgcaat ataatttacc
    13081 tgcgggaatt tatatctgta caatcaaagc gaacaaccaa caagattgtt cgaagaaaat
    13141 tattatttca aaatagacac agattatatt taaaatattt tgcatcagga attcgataaa
    13201 catgggttct tactgatgca aattataatt gtaatttaac tatcgtgatt ttagaatggt
    13261 cttgataaat aaaatttcaa tctttgtttt gaaataaata aattgaaatg aatgaaatag
    13321 aaatattaaa ttattcagaa gaaaatgcat cctatataaa agcgctgaat gttgaatggc
    13381 tcgaaaaata ttttgccgta gagccaaatg atgagctaca actatccaac cctcgaaccg
    13441 aaattattga taaaggaggt atgatatttt atgcttcgta caataacgaa attgtaggca
    13501 cagcaacatt attaaaagtg agtgataacg tttttgagtt atcgaaaatg gcggtgaccg
    13561 aacgtgccca aggattggga attggtaaaa aactgatgaa ccactgtttg aaaattgcca
    13621 cgcaaaataa tattcaaacc ctgattttat tttcgaatac aaaactggaa aaagcaattg
    13681 agatgtacag aaaatatggt tttgtagaag tgcccataaa tttaaatcat tacaaaaggg
    13741 ctaatattaa aatggaattg aaaatggaaa aaggaaggtt ttagtttcag agtccgcatt
    13801 tgattttgtg tatttatagc catgagaggt ttcagtttca gcgtcctcat ttatattttg
    13861 ttttttaaag gcatgagaga ctctgaaact aaaaccgtgg cattctaacc attaccctat
    13921 acgatgttga atcatatcct gaaccaaatc gaaaagttga ttgggtttta gtaagcgcca
    13981 gttaaaatca tcaaagtcgc caccatacac aaaataatag tcgatgccat ttttccgaaa
    14041 ttcctgagta acatgatgtc gaaaatttat ggctgcaatc catccaccat tgtcgcttat
    14101 atcttcgtac cactcttgat aataacaatc atccgagcca tgaaaattta gtacgttttc
    14161 tcctatcagc aaaaatttat ttatcccatt ctgtatcaac acatcggcaa tgtcacgttt
    14221 caaaaacata atgtcgttgt tgatagcatc gttccattcg ccaataagtt cgattatggc
    14281 aaaacctatt tcgtaatcga caaaaataat ttttatgtac aaagtaggcg aaccaatatc
    14341 gtcccactgg ggatgtatgt aatagttgta aatcgtgttt gtatattcaa attcgctata
    14401 aactacgttg tagaatggcg ataaactgtc atcggaggct atgtataaat cgcgccagtt
    14461 ataataaggt tcaatgtttt gcattgcgat ttattttgtg cgcatcatta tataagcatc
    14521 aacggctttt cgcacacttc cgtgttgttg cagtaaatca gcggccaatg tttcatcaat
    14581 tttcaattcg tccattatca tcatggtacc acgggctatg agtttattat tcgacaattg
    14641 catatccacc attttatttc cttttacatg tcccagccca atcattaccg aagtagaaat
    14701 catatttaat aataattttt gtgctgtgcc ggctttcatg cgtgtgctgc cggtaacaaa
    14761 ttcaggccct accaaacatt caattgcaaa atcggaatgc tttgcaacat ttgtatctgt
    14821 gttgcatgtg atgcatcctg tagttattcc attggcacgg cattgttgca atgcacctac
    14881 cacataaggg gtggatcccg aagcagcaat acctattacg gtatcgttaa cggtgagtgt
    14941 atacgctgcc aagtctttcc atccttgttc cacatcatct tcggcaaact ctacggcttt
    15001 gcgaattgcc acatcgccac cggcaataat tcctacaaca cgcccatgct caataccgta
    15061 tgttggcgga cattcgctgg catccacaat gcccagacga ccacttgtac cggctccaat
    15121 ataaaaaagg cgtccaccga ttttcatttt tgttattatt gattctacta aaggaacaat
    15181 ctttggaata caaagttcca ctgcgagagc cactttttta tcttcagcat tgatgccttg
    15241 taatagctcg gacacactca atttttcgag gtgctggtaa ttcgaagcgc tttcggtaac
    15301 ggtgttgccc atgattgtta ttagtacaat ttgggatatt gtgccggatt ggattgatgc
    15361 attaaactgt aaactgcctc ggttattttc tctgcattcg attttgaaaa ataatcacca
    15421 tcgctgccat aggccggacg atggtcgttt gccgacactg taagcggtga agcatctaaa
    15481 aattgataac cattttgtac ttccaatact tgttgcatca tgtaagcagt tgcaccacct
    15541 tccacatctt catcaacaaa aataatgcgg ttggtttttt gtaaactttt acaaatcaga
    15601 tgatttatat cgaaaggtat tagtgtttgc acatcaatta attccacact tattcccatt
    15661 tcggaaagtt gtttttgcga atccattgca actctgatgc aagaaccata agtaactatt
    15721 gtaacatcat cgccatccat taatacttca ggaatgccaa gtggcaaaca aaattcgcca
    15781 aggttgctgg gcattttttc tttaagccga tatccattta agcattcaat aattaatgca
    15841 ggattatcgc ttttcatgag ggtattatag aaacctgcgg cagtagtcat atcgcggggc
    15901 acaagaatat acataccacg caatgcgcct aaaataaatt gcatgggcga accactgtgc
    15961 caaacccctt cgaggcgatg tccacgtgaa ctcacaatca aaggggcttt ttgaaatcct
    16021 tttgtacgat aagaaaggca agccaaatca tctgttaaaa cattaatggc ataggcaaga
    16081 taatcgaggt attgaatttc ggcaatgggg cgcaaaccgc gtaaagccaa gcctataccc
    16141 tgccctatta ttgtggcctc gcgaatgccg gtatcctgca cgcgcaactc accatacttt
    16201 tgttgtaagc ctgcatacgt ttgattcact cctcctattt ttcctacatc ttcgccaaag
    16261 gcaagcatct cggggtacat atttaataac gcatcgaaat tggcttgtaa tatttcgcgc
    16321 ccatccatca tcacagcatt atcggaatat actggcgcta tactttttat tttaagagca
    16381 ttgttttctg tttcgctata taaatgcgac tcataccggt tatgcatatt ttgctcaaac
    16441 gcctgcatcc attgcacgag tgatttacgt tcatcgcttt tatcatttat ggttaagcgc
    16501 aggcattttt ttacggcagt tattattgct tttctatcca gttcatttgt atcatttaag
    16561 tcggcaataa ttttttcgac aaacactttt tgcgatgtat tttctttcac cttattcaat
    16621 accgccactg ccgaaattcg atcgttgttt atcgatgtgc aaaattcatc ccatgcccgc
    16681 ttcttacttt cgtttacatg ttgctttgct tccttttcta tttgctgcaa tacatcttcg
    16741 gtgcttatcc cattttcgag aataaactca cgcatttttg caatacaatc gtggtcgttt
    16801 tcccagtcaa ggcgttcttt acttttataa cgttcgtgcg agccacttgt actgtggccc
    16861 tgaggttgtg ttacatcagt aatgtgaaat aacacgggca tatgattttt gcggcaaaaa
    16921 tttattcctt ctttgtatac gctgcataat gtaggatagt cccatccttt ggcagtataa
    16981 attaaaaagc cgtctttgtc gccagtagtt gcaaaaccgc ttaaagcctc gctaatactt
    17041 tgttttatgg tttgatattt tttcgatacc gaaattccat agccatcatc ccacacactc
    17101 atagccaaag gaacttgcaa tacaccggca gcattcatgg tttcccaaaa caaaccttcg
    17161 cttgttgaag catcgccaat agtaccgaaa gcaacttcgt ttccgtttat agagaattta
    17221 gaaaaatcga cattgtttaa atcattatta ttacgatata attttgaggc ataagccaaa
    17281 ccaagcagcc tgggcatttg ccctgcagtt ggagaaatat cagccgaaga atttttcagc
    17341 tgcgtaaggt ttagccaatc acctttttca tctaaaagct ttgttgcaaa atgtccattc
    17401 atcaatcgtc cttgcgaaca agggtcggca tcagtatctg tatcggcata taattgtgca
    17461 aaatattgct gtaccgtaag caaccctgca gcgaacataa aagtttgatc gcggtaataa
    17521 ccggagcgaa aatcgccatg cttaaaaact ttggccatag caatttgtgc tacttcttta
    17581 ccatcaccaa atattccaaa ctttgctttg cccgtaagta cttcacgtct gccaagtaaa
    17641 ctcgcctgac ggcttatatt tgcaatccgg taatcatcaa ctaccgataa tttaaactgt
    17701 tcgaaactca ttagggtatt agcctcctgc tttgcctcct tcatataaaa aaatattttt
    17761 taatgatgta aagttaattt attacatgtt tagattccaa ataaaaggaa aaattgtgga
    17821 ttgggtttat gctaaaaatt atttggtgca attattgtcg ggctatttgc cgaaacagta
    17881 gttttgatgg caaaattatt ttatttttgt agtctattaa aactaattga ttggcatagc
    17941 atttatattg aattataatc taatgcacta ttatataaga taaattgaga tgagtgattc
    18001 ataccacttt ctaaatcatc aaaataaata ataaataaat cattaaaaat gaaacataat
    18061 ttacttctaa ttttattaat cacttttgtt acacctgctc tgtggagtca gtgtacaaat
    18121 acaaatgcta ccagttgtca atgtgccaat cagggcacca caaactgcga tttgttgcct
    18181 gatataatac cggcaaaaac tttattgtta gacaccggtg cttatggcat tattgaatat
    18241 acctacaacg accccaccat taaccgtgga cgtttaagag tatctgtttc tacgcctaat
    18301 attggacatg gtccgttaga aatacaaacc gattcaactt ttgtttgtgg caccgatacc
    18361 ataacaggca tacctccggc tacctgcacc aatacaggcc tgccgcctaa aatattagtt
    18421 cgtcaataca tttatcataa aaatggaaat gtaatgacca aaattaaaaa gcctgcaggc
    18481 agcatgactt atcaccctac ccatggccat atgcatgttg acaattgggg aacttatacc
    18541 ttacgcacct ccacatccaa tcctgatcca cttacctggc ccattgtagc aaccggacaa
    18601 aaactagctt tttgcctaat ggattttggt acatgtcaaa cttttattgg ccattgtgtt
    18661 gattcgaaca acactgtatt gggaaatgcc aatttcccaa atctcggttt gggaggtgct
    18721 gcttatacat gcagcaatgt taagcaagga atctcttcag gctacaccga tatttattat
    18781 caatacctcg atggtatgta cattcaactc gataccaata tttgtaatgg tgattattgg
    18841 atagtagttc aactcgatcc actaaactac ttcaccgaag aaaatgagaa caataatgtg
    18901 attgcagtgc catacacctt aaaaaatcaa agacccctac cagtaattaa tttaacaggg
    18961 aaccaaaata tttgccttgg aaatagtgtt aacttaacag cagcgggcgc aactacatat
    19021 acgtggtcac cagccagcgg gttgaataaa acaacaggaa ccattgttac tgcttcgcca
    19081 acttccacta caacgtatac agtaataggt agtaaaattg gtgctgggtg tatagataca
    19141 aattatgtta cagtgaattt ggtaacaaca gcaaacattt cggtaaccgg taacaatggt
    19201 atatgtacag gtagttctac aacgcttact gcatcgggtg ttaacaccta ttcatgggca
    19261 cctgcaactg gcttaaatgc aaccaa
//
LOCUS       DAORQA010000027        19201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_730_length_19201_cov_17.372976, whole genome shotgun sequence.
ACCESSION   DAORQA010000027 DAORQA010000000
VERSION     DAORQA010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 19201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19201
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_730_length_19201_cov_17.372976"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1237)
                     /locus_tag="PK736_03520"
     CDS             complement(188..1237)
                     /locus_tag="PK736_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32495.1"
                     /translation="MSVQTVNEIIGQYQISKGVTKSDLEKLASNSKTQSIQFAKPLSD
                     KEIDLLENVVFSKRPDISLRVYGHYGETCDLTFIQRIPSLRRISADCLMDAKGIEVVT
                     QLKNLEHLGVGIFNLDNFDFLDEINPNLTELYLHQTRSKKPKINSIQRFENLEYLYLE
                     GQQKGIEAVSYLKKLKKIVLRSISTNNIDYLKNLEHLWSVDIKLGGIKDLSGLTNLKS
                     IKYLELWQVRDLADLSFISKLETLQNIFIQSLKQLTRLPDFSNNKKLRRIYLENLKGL
                     NDLSTLKNAPSLKEFIYVLAENQEPENIIPALENPSIEKVFCKFGSDKKNNRFDELAK
                     QYKKTEYKHETFEYE"
     gene            complement(1356..1543)
                     /locus_tag="PK736_03525"
                     /pseudo
     CDS             complement(1356..1543)
                     /locus_tag="PK736_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964234.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1742..2521
                     /locus_tag="PK736_03530"
     CDS             1742..2521
                     /locus_tag="PK736_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRC32496.1"
                     /translation="MSKKIKILCWNVNGIRAIWKKGFPEWFAAQQPDILCLQETKAQK
                     EQLDNEVINFMDYKSYFCSAEKKGYSGVAIYTKQTPLNVNYGFNNPMFDSEGRVIEME
                     FENFVLFNVYFPNGGRGPERVKYKLDFYDELFKRAQEVRKKNKNIIVCGDYNTAHKEI
                     DLARPKENVKTSGFQPVEREWIDKIIGMDYIDIFREYNKEPAQYTYWDQITRARDRNV
                     GWRIDYYLVSKEMQSIISNATIHADVMGSDHCPIGLEICEL"
     gene            3496..5517
                     /locus_tag="PK736_03535"
     CDS             3496..5517
                     /locus_tag="PK736_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC32497.1"
                     /translation="MKKDIKKPVTPVKSPKAKADNAGETSLGMWGAIICAAFAFVIYA
                     NTLNHSWALDDFPTIYGNRITMQGVDSIPTLMSTAYWYGLDGKNDWLYRPLSMVMFAI
                     EWEIAPNTPALGHWVNVLFYSLTAFMLFKFLAELFKGKNILFPLSIALIYIAHPIHTE
                     VVANIKSRDEIMSFLFMMLTMYNVLLYARYGKMQKLILAFVFYTLALFSKESAITLIG
                     VIPLLLFFFTDVPYSKYAKPMMAVGAAAVIYLVARGNVLTSQTAQEGILISDNSLVST
                     DNVMMQKATAFYIIGLYIKLLFFPHPMSCDYSFNEIKIVGFDNWLALSSLIFHVAIGV
                     YALVRLPKKDPVSFGILFYLITISLVTNVLFLTRSTMADRFLYTPSLGFCIVVVVLLE
                     RLLKINFEEKIYSLSRIFTFNTTYTAIVSVVLIAASLKTFSRNRDWESDTTIFSTDSR
                     HAPGSARIHYLYGNHFLQELNQKKVPQEQQENYWNIAVAELKKSIECHADYMESYMGL
                     GDCYARKGMFKEAFDIYNVAIKRKPDFANAHNSLGNCYFKAGQYDNAILALQQAIKIQ
                     PDYAEAYNNMGSAYFSKGDYANAVTAFQTAIRISPTYVDAHKNLGSAYGTMKDYDKSI
                     ASFLNALKFDPNSAEINRYLGLTYQFKGDMTNGSRYLNRAYELDPSLKK"
     gene            5602..7131
                     /gene="guaA"
                     /locus_tag="PK736_03540"
     CDS             5602..7131
                     /gene="guaA"
                     /locus_tag="PK736_03540"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRC32498.1"
                     /translation="MHQTILILDFGSQFTQLIARRVREQNVYCEIIPYNKPFELGAHV
                     KGVILSGGPSSVRDVDAPSVDTIALRKKIPVLGVCYGAQLMALQAGGNVAPSTKREYG
                     RAALQLSAHESALMQDVHPQSQVWMSHADTILHAPENTTVLASTNDVKCAAYQFDNEN
                     NFGVQFHPEVVHSIEGKTIIKNFVINICGCKGDWTPNAFVEESIENIRGIVGNDRVLL
                     GLSGGVDSSVAALLLSKAIGKNLYCIFVDNGLLRHNEFQQVLDAFAHFDLNIIGVDAA
                     QQFYDALKGITDPEEKRKSIGKTFIDVFDAEAHKIQDVKWLAQGTIYPDVIESVSVKG
                     PSATIKSHHNVGGLPEKMKLKVIEPLRSLFKDEVRLVGDTLQLDHNILHRHPFPGPGL
                     GIRILGDITAHKVEILQKVDDIFINGLKSHNLYEKVWQAGSILLPVKSVGVMGDERTY
                     ENCVALRAVTSVDGMTADWAHLPYEFLAHISNEIINSVKGVNRVVYDISSKPPATIEW
                     E"
     gene            7203..7955
                     /locus_tag="PK736_03545"
     CDS             7203..7955
                     /locus_tag="PK736_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32499.1"
                     /translation="MRKLEPLFELIAGILFIFLGIEIVAYFGKFHMPLKVIGLVGFAA
                     VLYGGFLLLTNLIVYSIYFFYKFLYDKKKLNQPDADKPLSVSAKNIIRILALVTICFF
                     LYEARTIENVLGGISLLQKGIWCGIIVAVLLLFVVFKKIKLLRQNFAIGYSVFILIPL
                     ACCMLGIATLSLLNRTGMKKEITYSKMVTKKADNMRYGTKWIFIYNKNNEERLEVSDG
                     FYKSVAKNDTVILNCKKGNFGYDVVVKISKKN"
     gene            complement(7980..8375)
                     /locus_tag="PK736_03550"
     CDS             complement(7980..8375)
                     /locus_tag="PK736_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32500.1"
                     /translation="MMIKKSLLLGAISGLLAGIACVIYQKVYASSLGADFATIVKPMQ
                     VVITTIISGLIAGTGFWLCNKWFKGNGEIIFNFVFAILSFASILPAFAFKLPLDAEMP
                     ELFPGLVVPMHFFSALAWFTLKPLFFKSA"
     gene            complement(8378..8908)
                     /locus_tag="PK736_03555"
     CDS             complement(8378..8908)
                     /locus_tag="PK736_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRC32501.1"
                     /translation="MKKIIPAFLVLVSMFAFLAFAPADAWKIGTKNNVTFSTSGVSGI
                     FKTLTGKIVFDEQNLSTAIFDISIDVNSINTGNGMMNTHAKGDDWFESAKYPSIKFTS
                     KKVEKTPTGYLATGSLQMHGITKEMSLPFTFTKNETGGIFEGTFSVKRNDFKIGEAGG
                     EVGDEIKIKVTVPVTK"
     gene            complement(8974..9546)
                     /locus_tag="PK736_03560"
     CDS             complement(8974..9546)
                     /locus_tag="PK736_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRC32502.1"
                     /translation="MKKISILLVATVVALSSFKAVEMWKMDKAHSALTFTVTHMGISD
                     AVGTFKNVDVTIKSEKADFSDAIFEFTAEAASVNTGNEMRDKHLMSPEFFDVEKFPKV
                     SFVSTGIKNVSKNTYKLSGNLTLHGVTKPVVLDMLYRGTIENPMSKATTSGFQVTGSI
                     KRSDFAFGSGMPEALLSDVVTIKADGEFTK"
     gene            9703..10068
                     /locus_tag="PK736_03565"
     CDS             9703..10068
                     /locus_tag="PK736_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC32503.1"
                     /translation="MKKKPIPESSQVCTKRFLAIRDTMELLSGKWKIQIIGSLMQKGK
                     MRFMDLLREVDGIAAKMLSKELQELELNELVTRTVCNTKPITVEYEITHYGKTLEKVI
                     DEMAQWGYLHRKRLFKKSK"
     gene            complement(10278..11048)
                     /locus_tag="PK736_03570"
     CDS             complement(10278..11048)
                     /locus_tag="PK736_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HRC32504.1"
                     /translation="MDYPKRIITKGETDKKIVKAIQTQLNAKGCGPVAVDGDFGPGTV
                     SAVKLFQTRNADSNGSQLKADGKIGAITWSTLFGEETVPVIDTTSSAMLQKVLEIAVS
                     QIGVSEDPNNKNRGPEVDEYLRRAGLEPKGQHYSWCMAFVYWCFDEAAKSLGKKNPLV
                     KTPGCLKQWNETTCPKVKMADAKNNPSLVKPGFVFIIDHGKGLGHTGIVESVNGGYLN
                     TIEGNTSQAGSREGTGVMRLSRRKIGDISKGFINAGKA"
     gene            complement(11116..12201)
                     /locus_tag="PK736_03575"
     CDS             complement(11116..12201)
                     /locus_tag="PK736_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32505.1"
                     /translation="MKRLRILTLCSLSIALAIFFFKNETPSTISQGDGLVLKKEWLAE
                     NKNPATPKQYCRPADQTFLTFPEWFLVFSPDEQARFFKHTTATTFPYTTHTTQIWQSY
                     KIVCDQIRDNFVYNGGYHFMIWVIGSSATVEYTIKAWYEKIIGRLTDTYEVITDEDIF
                     NATYTQNYVDFIKDRPWYEFNFKKELSTLYKTTTIFDTYFIRKIERKYILTSELIVKI
                     VYGKLIGLGTASVYEAALPTTEVLVDSLVANVAPFKILKTYENKSALISLPRYNKFND
                     AACKLAQQGFTFKEVAGNNSAILITVLAPVDTRIAYKNCQTIFIQSISSQRQMQRIAI
                     AAPVKDLSTLLLQLKNDELIIEHIFDF"
     gene            complement(12204..13064)
                     /locus_tag="PK736_03580"
     CDS             complement(12204..13064)
                     /locus_tag="PK736_03580"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRC32506.1"
                     /translation="MDNTKSEFKAVFSNPKWSDDFYRFLQVIYHLYPEDKFHQLIAAT
                     AKEKNSDEEIYKAVQQGLPKIKTFLSELTYALPALKKQKKEMANQVLQLLGERKSING
                     YLEIGSTGRYISELRKHIKLSGNIFISNDIAPTNSIADMMERGQWSKLGTFFSLDNYQ
                     PIPDSIIKDESLDLITCHIGLHHCLHNLLDTYLNSIKRTLRKGGLFIMRDHDVKSADM
                     ATFVSLVHTVFNLGLNVKWEVDKSEFKSFKSIEEWSKIICSHGFIDSGKRILQENDPS
                     DNTLVAFEKI"
     gene            complement(13068..14810)
                     /locus_tag="PK736_03585"
     CDS             complement(13068..14810)
                     /locus_tag="PK736_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRC32507.1"
                     /translation="MAIKDKLPVSYLLFTNRGRISRGTYWTVSIFIWTTFYVLFNALE
                     FFISYSSTLIIYPLLFWAIIATATKRLHDNNLSAWWLLLIAIPVAGPLLLFYFLGLKK
                     GTLNKNKYGVNPKLAADYFENGAAQSIPHLKTNERIVNDVTQLNPVIVAKVETPRTIE
                     ELQNIIKNNNAVSIGGGRFSMGGQTASAQTIHIDMRSLNKIIDFSPNHKTIKVEAGIR
                     WCDIQQYIDVENLSLKIMQTYANFTVGGSLSVNCHGRYIGLGPLILSVKSIDVILQNG
                     EMKHASLTENAELFFACVGCYNAVAVIANVELELTENVAVERINTTMKIGEYKNFFFE
                     KVRNTSDVIFHNGDIYPPAYNMVRAVSWVETKKKTTEPTRLMPLAASYPLERYFIGAF
                     SKSKFGKWRRQYIYDPILFARNKVHWRNYEAGYDVAELEPESRKKSTYVLQEYFVPVN
                     MFDVFAARMKEIFVRHNVNVINVSIRHALPDSGSLLAWAHEEVFAFVIWYKQNTDEAS
                     KNKVGVWTCELIDAVLSVKGSYYLPYQAHATSKQFHAAYPNAKKLFEIKSQLDPDYKF
                     RNILWDTYYRKTEK"
     gene            complement(15014..15174)
                     /locus_tag="PK736_03590"
                     /pseudo
     CDS             complement(15014..15174)
                     /locus_tag="PK736_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199591.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
     gene            complement(15210..15692)
                     /locus_tag="PK736_03595"
     CDS             complement(15210..15692)
                     /locus_tag="PK736_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRC32508.1"
                     /translation="MIPEIKNLSAKYLIGNHLEMCMASNRTIELWKNFMPRREEIKNT
                     NGVDLFSVQIYDTKFNFLHFDINANFEKWAAVEVADFTNVPDGMQTLILPQGLYAVFL
                     YKGAAEEGEKTFRYIFEAWIPNSIYQLDNRPHFEILGSKYKHNDPDSEEEIWIPVKHK
                     "
     gene            complement(15753..16697)
                     /locus_tag="PK736_03600"
     CDS             complement(15753..16697)
                     /locus_tag="PK736_03600"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HRC32509.1"
                     /translation="MEQTNFNNIVAHPFLKWAGGKSQLLEKFRGIYPPELTDHKIKNY
                     YEPFLGSGAVFFDVVQKYQIENAFLYDINDELIMTFKVVQKDVDALLQLLHRYQKKYS
                     GLDNEKRKSFFYDERTKYNLNRNKTDYNSYSDKWFARAAQFIFLNKTCYNGLYRVNSK
                     GEFNCPAGEYANPNICDEQNLLAANRVLQIAEIKKAVFNNLLNDIKPKSFVYFDPPYR
                     PISKTSSFKAYAKNDFGDDAQKQLASLFKKLDKKGVKVMLSNSDPKNIDASDNFFDEL
                     YSGFNIERIPARRIINSNATRRGAINEIVVTNYATKGI"
     gene            complement(17151..18500)
                     /locus_tag="PK736_03605"
     CDS             complement(17151..18500)
                     /locus_tag="PK736_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC32510.1"
                     /translation="MEKIWLIIQREYITRVRKKSFILLTLLGPLLMGGLFAGVIYMQT
                     HSDKTISNIQVVDETQYAEYARTLKSSESVKYFADTASFSNAREKFNKVNFYGLLHIY
                     QSAENKDSTVFQLYTEKLVSLGTVSRIEDDIQKIYDEQQLVKNNIDPLLLKSLHKSIS
                     ISTKTLQGDGKGDSEVSAGAAAAIAFGGGILIYMFIFIYGAQVMRGVMEEKSSRIVEV
                     IMSSVKPFQLMMGKIIGIALVALTQFILWGLLTMLVSTVVTSLMTDKEAMAQQIAQSQ
                     NSDGNKTGMANPGMNMQPGGNANQSLQVIEQFKSFNFGKIIGLFIFYFLFGYLLYASM
                     FAAVGAAIDAETDVQQFMLPITIPLIFAYIAAASIISNPDSALGLWCSIIPFTSPIVM
                     MVRVPYDPPMWQMLLSIFLLIITFITFTWIGAKIYRTGILMYGKKVTWKEMYKWLRY"
     gene            complement(18531..>19201)
                     /locus_tag="PK736_03610"
     CDS             complement(18531..>19201)
                     /locus_tag="PK736_03610"
                     /inference="COORDINATES: protein motif:HMM:NF025109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC32511.1"
                     /translation="GFTKKMEVGEQALYLARLKGMSRSDAMTELRYWFEKFELKGWWK
                     KKIEELSKGMAQKVQFITTVIHKPRLLILDEPFTGFDPINANLIKDELLEMKSKGVSI
                     VLSTHRMESVEELCTHIALINKSKVVLNGSVKEVRKDFKSNIYEIHYAGNNISLANSL
                     WAGFELISNVKHEDENIATVKLIDDATPNQLLHALIDNIEIHLFREVTPTMSDIFIKA
                     VNNS"
BASE COUNT         5732 a         3440 c         3507 g         6522 t
ORIGIN      
        1 aagctgtttc ggccgccaaa atttttaaaa ggtaggtttt ttcacatcct gacgttttgc
       61 agctacaata agttggcgat ttgggagacg aaaactttct gctactactg aacttgatac
      121 aaagcacaat gcttcattta accactgaat cgccaatttc ttgtaggtgc tgttatgcac
      181 agtaattcta ttcatattca aaggtttcgt gtttatattc tgtcttctta tactgttttg
      241 ctagttcgtc aaagcgattg tttttcttgt cgctaccaaa tttacagaat accttttcaa
      301 tagatggatt ttcaagtgct ggaattatat tttcaggttc ttggttctcc gccaaaacgt
      361 aaataaattc ttttaaactt ggtgcgtttt tcaaagtcga caagtcattc aatcccttta
      421 agttctcaag ataaattcgt cttaatttct tgttatttga aaagtcaggc aacctagtta
      481 attgttttag ggattgtata aatatgttct gaagggtttc caattttgaa atgaacgata
      541 agtctgccaa gtctcttact tgccataatt ccaaatactt tattgattta agatttgtca
      601 aaccgcttaa atcctttatt ccacctaatt ttatgtctac tgaccataga tgttctaaat
      661 tttttagata gtcaatattg tttgttgaaa ttgaccttaa gacaattttc ttcagttttt
      721 tcaaatagct tactgcttca ataccctttt gttgtccttc caggtacaag tattctagat
      781 tttcaaatct ctgaatgcta tttatttttg gtttttttga ccttgtttga tgaagataaa
      841 gttctgtaag gtttggattt atttcgtcca gaaaatcgaa gttgtccaag ttgaatattc
      901 caactcctaa atgttctaaa ttcttaagtt gagttacaac ttctattcct ttagcatcca
      961 ttaaacaatc agcagaaatt cttcttaagg atggaattct ttgtataaat gtcaagtcac
     1021 aagtttcgcc ataatgtccg tacactcgca aagaaatgtc gggtcttttt gaaaaaacca
     1081 cattttctaa aaggtcaatt tccttgtcag ataaaggttt tgcaaattga attgattgcg
     1141 ttttagaatt tgacgcaagt ttctcaaggt cgcttttggt tacgccttta gaaatttgat
     1201 actgtccaat tatttcgttt actgtctgta cgctcatttt ttattattgt gcataacttg
     1261 cttatacccg ctacatcata gcgcatatac aacagtattc tgcggatatg cgctactttt
     1321 ttgttttttt tatatggcaa tagtaatatt ttttatcata atttatcgaa gggtgaaata
     1381 atattttgta tcgatttggt acttatatgt gtatatattt ttgtggttcg gcttctgcta
     1441 tggcctcgta gttgttgaat ataacgtagg ttggtgccac tttagttaag tgtgttgcat
     1501 agctataacg taaccaatgc aagcaaactg gtttgtttat tttagttatt aatgaatgat
     1561 atgcactcta caaaagaata aataaaacta tgctgcaata tgattaaaaa aataaatatc
     1621 gcttgcaaag gtttttgatt cattcaaaca gacatttgta gttttttgta cgatgcactt
     1681 cgtttttgcc cgagcccaga aaatattttt ttatgtttac atcattaaaa caataaagcc
     1741 aatgtcaaaa aaaataaaaa tactttgttg gaatgtgaat ggtattcgtg ccatttggaa
     1801 aaaaggtttt cccgaatggt ttgcagcaca gcaacccgat atactttgcc tgcaggaaac
     1861 caaagcacaa aaagagcaac tcgataacga agtgatcaac tttatggatt acaaaagtta
     1921 tttctgctcg gccgaaaaaa aaggatattc gggtgtggcc atttatacaa aacaaacacc
     1981 attgaatgta aattatggtt tcaataatcc gatgttcgat agtgaaggcc gggtaataga
     2041 aatggagttt gaaaattttg ttttgttcaa tgtgtatttt ccaaacggtg gtcgtgggcc
     2101 tgagcgtgta aaatataaac tcgattttta tgatgagtta tttaagcgtg cacaggaagt
     2161 gcgtaagaaa aataaaaata taattgtgtg tggcgattac aacactgctc acaaggaaat
     2221 tgatttggcg cgcccaaaag agaatgtaaa aacatcgggc tttcaacctg ttgagcgcga
     2281 atggatagat aaaattattg gtatggatta cattgatatt tttcgtgagt ataataaaga
     2341 accagcgcaa tacacttact gggaccaaat tacccgtgcc cgtgatcgca atgtgggatg
     2401 gcgaatcgat tactatttgg tatcgaaaga aatgcaaagc atcatcagca atgcaaccat
     2461 tcatgccgat gtgatgggca gcgatcattg tcctattggg ttggaaattt gtgaattgtg
     2521 aattgtgaaa tgtgaattgt gaatggtcaa ttgtgaattg tcagaagggg aaagtcaaaa
     2581 ttaaatgccg aagctttttg tcaaaagaat ttattctctt cgttgcatta ttgttgacta
     2641 agtagaagtg gagatttatg agtagagcta aaatttaaaa aagaataatt tatgattcaa
     2701 tggagttgta aaatttatat tcatcactac gcatctacat ccttttttaa agaccgtgat
     2761 tgattttgtt tacaaccaac catgccccat ttgtgatgta attatactaa gagtagttag
     2821 aacttcgaat ggaaataaaa cgtactttta ttaatgccga acaacttcaa tagcatatat
     2881 ttccaaaacc taagttgttt cagtatacgt tggcacaatc ttatttttgc acagttagtg
     2941 atcagccgaa aagcatgttt cgaaatgtat gttattcaac tttcgaatac ggcaacataa
     3001 tttatttttt aaaccaccac taaaaacttt tataactact cagtactcaa gcttaattta
     3061 attgagttaa gagaattgtt ttttatcccg cagggattac cgcttggtaa aaaacaaaga
     3121 tgctgtgtat tatgtgccgt aggcactacc cattttgctg cgttgtatta tcgtcattgg
     3181 gtagtgctta caggacaaaa accgggaggg gttgcttctg ttaccaagcg gtagtcccta
     3241 cgggacatgc ataagttgaa aacagctttt aaaatttagc gctattcata atggctggtt
     3301 catttcgcaa ttgtgtatag ctaaactaca gtttgtaaaa tttatatttc taaaggcata
     3361 acttaatggt ataaggcttt agccttgtta ttcgcaattt atataatgtt caaatattta
     3421 aaaaggacta aaggcattct gattagttac aaatattcgt actatattta gccacttttt
     3481 taaaacaaaa tattaatgaa gaaggatata aaaaagcctg taacgccagt aaaatcacca
     3541 aaggcaaagg ctgataatgc aggcgaaacc tcactgggta tgtggggtgc tattatttgt
     3601 gctgcatttg catttgttat ttatgccaac acactcaatc attcgtgggc gcttgacgat
     3661 tttccaacca tatacggcaa ccggattacc atgcaagggg ttgatagtat tccaacgtta
     3721 atgtcaacgg cttattggta tggcctcgat ggaaaaaacg actggttgta tcgccctttg
     3781 agtatggtaa tgtttgccat tgaatgggaa atagcaccca acactccggc actgggtcat
     3841 tgggtaaatg tgctttttta ttcgcttaca gcatttatgc tttttaaatt tctggccgaa
     3901 ttatttaaag gcaaaaatat tctctttccg ctttctattg cgctcattta tattgcacac
     3961 ccaatacata ccgaagtggt ggcaaacata aaaagtcggg acgaaattat gagtttcctt
     4021 tttatgatgc ttaccatgta taatgttttg ctttatgcca ggtatggaaa aatgcaaaaa
     4081 cttattcttg cttttgtgtt ttatacgctt gctttgtttt cgaaagaaag tgccatcact
     4141 ttaattggtg ttataccgtt gttgttattt ttctttaccg atgtgcctta ttcaaaatat
     4201 gccaaaccga tgatggctgt gggtgcagcg gcagtaatat atttagtggc gcgtggcaat
     4261 gtgcttacat cgcaaacagc acaggagggc attttaattt ccgataattc gctggtctct
     4321 accgataatg taatgatgca aaaagctacg gcattttata ttataggctt atacattaag
     4381 ctgttatttt ttccccatcc tatgtcgtgc gattattctt tcaacgaaat aaaaattgta
     4441 ggtttcgata attggctggc gctttcatct ctcatctttc atgttgctat tggtgtgtat
     4501 gccctggtaa gattacccaa aaaggatccg gtttcgtttg gcattctgtt ttatcttatc
     4561 actatttcgc ttgttaccaa tgtgctgttc cttacccgaa gcaccatggc cgacaggttt
     4621 ttgtatactc catcgttggg attttgtatc gtagtggtgg tgctgcttga gcggttgttg
     4681 aaaataaatt tcgaagaaaa aatttacagc ctctcccgta tttttacttt caatactacg
     4741 tatacggcca tagttagtgt tgtgttgatt gcagcttcgt taaaaacctt ttcgcgcaat
     4801 agagactggg aaagcgacac gaccattttt tcgaccgata gcagacatgc acccggaagc
     4861 gcccgaattc attacctgta tggtaatcac tttttgcagg aattaaatca aaagaaggtg
     4921 cctcaggaac aacaggaaaa ttactggaat atagctgtgg ccgaattgaa gaagagtatt
     4981 gagtgccatg ctgactatat ggaatcatat atgggccttg gcgattgtta tgcacgtaag
     5041 gggatgttta aagaagcttt cgatatttat aatgtagcca taaaacgcaa acccgatttt
     5101 gcaaatgctc ataacagttt gggtaattgt tatttcaaag ccgggcaata cgataatgcc
     5161 attttagcgc tgcaacaagc aataaaaatt caacccgatt atgccgaggc atataacaac
     5221 atggggtcag catattttag caaaggcgat tatgcaaatg ctgtaacggc ttttcaaacg
     5281 gctataagaa tttcgccaac ttatgttgat gcacataaaa atttgggcag cgcttatggc
     5341 accatgaagg attacgataa atcgatagca agttttttaa atgctctaaa gtttgatccc
     5401 aacagtgcag aaataaatcg ataccttgga ttaacctatc agtttaaagg agatatgact
     5461 aacggcagcc gataccttaa tcgtgcgtat gaattggatc catcattgaa gaaataaaaa
     5521 aaactcactc agtatattta aacataaatg ggaatattat ttttggaaac ataaattaat
     5581 atttcaaaga caaaaaaata aatgcatcaa acaattctca tactggattt cggttcacag
     5641 tttacacaac ttatagcgcg cagagtgcgc gaacaaaatg tgtattgcga aattattcct
     5701 tacaataaac cgtttgagtt aggtgctcat gtaaaaggcg taatactgtc gggagggcca
     5761 tcatcggtgc gcgatgtgga tgcaccttcg gttgatacca ttgcgctacg aaaaaaaata
     5821 cctgtgcttg gtgtttgtta tggtgcgcaa ctgatggctt tgcaggcagg tggaaatgta
     5881 gcaccctcaa ccaaacgtga gtatggtcgt gctgcactgc aattgtcggc acatgagtct
     5941 gcattgatgc aggatgttca tccgcaatcg caagtgtgga tgtcgcatgc cgataccatt
     6001 ttgcatgcac ctgaaaacac tacagtactt gcctcgacaa atgatgtaaa atgtgccgca
     6061 tatcaattcg ataatgagaa taattttggt gtgcagtttc atcccgaggt ggttcattct
     6121 atcgaaggca aaacaatcat aaaaaatttc gtgataaata tttgtggttg caaaggcgac
     6181 tggacaccca atgcatttgt agaagaaagt atagaaaata ttcgcggcat agttggcaac
     6241 gatagagttt tgctcggcct ttcgggcggt gtcgattcca gtgttgccgc tttgttatta
     6301 agcaaggcca taggcaaaaa tttgtattgt atttttgttg acaatggttt gttgcgccat
     6361 aacgaatttc aacaagtgct cgatgcattt gcacatttcg atttaaatat aattggtgta
     6421 gatgctgcac aacaatttta cgatgcacta aaaggcataa ccgatcccga agaaaaacgt
     6481 aaatcaatag gcaaaacttt cattgatgtg tttgatgccg aagcgcataa aatacaagat
     6541 gtaaaatggt tggcacaagg aactatatat cccgatgtaa ttgaaagcgt gagtgtgaaa
     6601 ggaccttcgg ctacaataaa atcgcatcat aatgtaggtg gacttcccga aaaaatgaaa
     6661 ctcaaagtga ttgaaccttt gcgcagtttg tttaaagatg aagtgcgcct ggtgggcgat
     6721 acattgcaac tcgatcacaa tattctgcac cgccatcctt ttcccggtcc tggattgggc
     6781 attcgcatac ttggtgatat taccgcacac aaagttgaaa ttttgcaaaa ggtggacgat
     6841 atttttatca atggattgaa gagtcataac ctgtacgaaa aagtatggca ggccggaagt
     6901 attttattac cggtgaaaag tgtgggtgtg atgggcgatg aacgcactta cgaaaactgc
     6961 gtagcattgc gtgccgtaac ttctgtagat ggcatgactg cagattgggc acatttgcct
     7021 tacgaatttt tagcacacat ctccaacgaa attattaact cggtgaaagg tgtaaataga
     7081 gtagtgtacg atattagtag taagccgcct gcaaccatag agtgggagtg atgctctttt
     7141 tagaattacc atttgtacaa tttattttgt tgtaattatt tttactcaaa taaaagtttc
     7201 caatgcgaaa attagaacca ctgtttgaat taattgccgg catacttttt atttttttag
     7261 gcatcgaaat agttgcctat tttggtaagt ttcacatgcc actcaaagta attggtttgg
     7321 tgggttttgc tgcagtattg tatggaggtt ttcttttgct caccaatctt atagtttact
     7381 ccatttattt tttctacaaa tttctctacg acaaaaaaaa gttgaatcaa cccgatgctg
     7441 ataaacctct gagtgtttcg gcaaaaaata taattcgcat attagcatta gtgacaatat
     7501 gttttttctt atatgaagcc cgcactatcg aaaatgtttt gggcggtatt tctcttttgc
     7561 aaaaaggcat ttggtgtgga attattgtgg ctgtgctatt attatttgtt gtctttaaaa
     7621 aaataaaatt actgcgacaa aattttgcta tcggctactc agtttttatt ttaataccat
     7681 tagcttgttg tatgctgggc attgctactt tgtcattatt gaatagaaca ggaatgaaaa
     7741 aagaaattac ctatagcaaa atggtaacca aaaaagctga taatatgcga tacggtacta
     7801 aatggatttt tatttacaat aaaaataacg aagaacggct ggaggtgagt gatggttttt
     7861 ataaatctgt agccaagaat gatacagtaa tactgaattg caaaaaaggc aactttggct
     7921 atgacgttgt agtaaaaata tcgaagaaga attagcagag aaacttgttg taaaaaaaac
     7981 taagcgcttt taaagaataa gggctttagt gtgaaccacg ccagtgcaga aaaaaaatgc
     8041 ataggaacaa ccaggccggg aaataattca ggcatctctg catcgagggg aagtttaaaa
     8101 gcaaatgcag gcaaaatact tgcaaacgaa agaatagcaa aaacgaaatt gaaaattatt
     8161 tctccatttc ctttaaacca tttattgcat aaccaaaatc ctgtgccggc aattaatcca
     8221 cttatgattg tagtaattac cacttgcatt ggttttacaa tagttgcaaa atcagcaccc
     8281 aacgatgagg catatacttt ttgatagatt acacatgcaa tgccggcaag taaaccagaa
     8341 atagcaccga gtaaaagaga tttttttatc atcattttta tttagtaacg ggtacagtaa
     8401 cttttatttt tatttcatca ccaacttcac cacccgcttc accaatttta aaatcatttc
     8461 ttttaacaga gaatgttccc tcaaatattc cacctgtttc attttttgta aaagtgaatg
     8521 gcaagctcat ctcttttgtt atgccatgca tttgcaaact tcctgttgct aaatatcctg
     8581 ttggtgtttt ttcaactttc tttgaagtga attttatgct tggatatttt gctgactcaa
     8641 accaatcgtc accttttgcg tgtgtgttca tcatgccatt accggtatta atgctgttaa
     8701 catcaattga tatatcaaaa atggctgtag acaaattttg ttcatcaaaa acaattttac
     8761 cggttagtgt tttgaaaatg ccacttaccc cggaagtgct aaatgtgaca ttgttttttg
     8821 ttcctatttt ccatgcgtct gcaggtgcaa atgctaagaa agcaaacata gaaacaagta
     8881 ctaaaaatgc aggaattatt tttttcataa actttaattt aattgtaaaa aaaagactga
     8941 gcgcaatgcc cagtcttttt tatgctaaga taattatttt gtaaattctc cgtcagcctt
     9001 tattgtaaca acatcgctta acaatgcttc gggcatgcca ctgccaaatg caaagtcaga
     9061 acgtttgatg ctgcctgtaa cctgaaaccc gcttgtggtg gccttcgaca ttggattttc
     9121 aattgtgcca cggtatagca tatctaatac tacaggtttt gtaacaccgt gcagtgtcag
     9181 gtttccagat aatttgtatg tgttcttact aacatttttt atacctgtac ttacaaaaga
     9241 tactttgggg aatttttcta catcaaaaaa ttcagggctc atcaaatgct tatctctcat
     9301 ttcgttacct gtattaactg atgcagcttc ggctgtaaat tcaaatatgg catcgctgaa
     9361 atcggctttt tcggatttaa ttgttacgtc aacatttttg aatgtaccaa ccgcatcact
     9421 tatgcccata tgggtaacag tgaaagtcaa tgctgaatgt gctttgtcca ttttccacat
     9481 ttcaactgct ttgaacgatg ataacgccac aacggtggct accaataaga tactaatttt
     9541 cttcataatt tttttgtttt ttgtttgagg ctgcaatgat atgaaaaata gtttactttt
     9601 gttaatgatt tccttttagt aactggttac ctttaagaaa ctcagtctga taaatgtaaa
     9661 gttcgtattt atgtttgtat cgaaattaat atcaaaatag aaatgaaaaa gaagcccata
     9721 cccgaatcat cacaagtatg taccaaaaga tttttagcca ttagagatac tatggagttg
     9781 ctatcgggca aatggaaaat acagattata ggttcgctca tgcaaaaagg gaaaatgcgt
     9841 tttatggatt tgctgcgcga agtggatggt attgccgcca agatgttgtc gaaagagctg
     9901 caggaacttg agttaaatga attggtaacg agaacagttt gtaatacaaa acctattact
     9961 gtagaatatg aaataacaca ttatggcaaa acgctcgaaa aggtaatcga tgaaatggcg
    10021 caatggggtt atttgcatcg taagcgatta ttcaaaaaat ctaaataaaa aaaatggata
    10081 tgtttttcac atatccattt tttttgttga cggaattatt tttaaaaaga ctaaattgtt
    10141 aagcagattt ttttttgatt agaaactatt tcacaaacaa agttcaatat caaaaatgca
    10201 aattccgaac attgataaag attatacaat ctgaaaaaaa ataattttac cctgtgcagc
    10261 attaaaacag gaataattta agctttgccg gcattgataa atcctttgga aatgtcacca
    10321 atctttcttc ttgaaaggcg cattactcct gtaccttcac ggcttcccgc ctgactggtg
    10381 ttgccttcta tagtattaag gtagcctccg tttaccgatt caacaattcc agtatgtcct
    10441 aaacctttac catgatcgat aataaaaaca aaaccaggct tcactaatga aggattattt
    10501 tttgcatcgg ccatttttac ttttgggcat gtagtttcgt tccactgttt gaggcaacct
    10561 ggagttttta ccagagggtt ttttttgcct aatgattttg cagcctcatc gaaacaccaa
    10621 tatacaaatg ccatgcacca gctataatgt tgacctttgg gctcaaggcc tgccctgcgc
    10681 aagtattcat ctacctcagg tcctctgttt ttgttatttg gatcttctga aacgccaatt
    10741 tgcgatacag caatttctaa aaccttttgc agcattgcag atgatgttgt gtcgattaca
    10801 ggcacggtct cctctccgaa aagggtcgac catgtgattg ctccaatttt gccatcggct
    10861 tttaattgac taccgttgct atcagcattt cgcgtttgaa ataatttcac agccgatact
    10921 gtgccgggcc caaaatctcc atcaactgca acagggccgc aacccttagc attaagttgt
    10981 gtttgaattg cttttacaat ttttttgtct gtttcacctt tggtgataat tctctttgga
    11041 taatccataa tgatttttta ttaaatggtt aaatgatatg tgtcaaaagt attttaaatt
    11101 taataggata caacattaaa aatcgaaaat atgttcgata attaattcat cgtttttaag
    11161 ttgtaataat aaagtactca aatcttttac cggagctgca attgctattc gttgcatttg
    11221 cctctgcgat gaaatggatt gtataaaaat agtttgacaa tttttataag ctatgcgggt
    11281 gtcaacaggc gctaaaaccg taatcaaaat tgccgaattg ttaccggcaa cttctttgaa
    11341 tgtaaaccct tgttgcgcca gcttgcaagc agcatcatta aacttgttgt agcgtggcaa
    11401 actaattaag gccgatttgt tttcgtaggt ctttaatatt ttgaaaggcg caacatttgc
    11461 aactaatgaa tctactaata cttcagttgt gggtaacgct gcttcataaa ccgatgcggt
    11521 gccaagtcca atcaatttgc cataaactat tttaacaatc agttccgatg tcaaaatata
    11581 tttacgttct attttacgaa taaaataggt gtcgaaaatt gttgttgttt tataaagtgt
    11641 agataattcc tttttgaaat tgaattcgta ccacggcctg tcctttataa agtctacata
    11701 gttttgtgta tacgttgcat taaatatatc ttcatcggta atcacttcat aagtatcggt
    11761 aaggcggcca ataatttttt cgtaccatgc ttttatagta tattctacag ttgcactcga
    11821 gcctattacc caaatcataa aatgataacc accattgtat acgaagttgt cacgaatttg
    11881 gtcgcaaaca attttatagc tctgccatat ttgtgttgtg tgagtggtat atggaaaagt
    11941 agtagcggta gtgtgtttaa aaaatcgtgc ctgttcatcg ggactgaaaa ctaaaaacca
    12001 ttcgggaaaa gtcaaaaatg tttggtcggc cgggcggcaa tattgttttg gtgtagcagg
    12061 gtttttattt tccgcgagcc actctttttt taataccaat ccatctcctt gcgatattgt
    12121 agaaggggtt tcatttttga aaaagaatat tgccaatgca atagaaagag aacaaagtgt
    12181 tagtattctt agccttttca tttttatatt ttttcgaatg cgacaagagt attatccgaa
    12241 ggatcgtttt cttgtaatat ccttttgcct gaatctataa acccatggct gcaaattatt
    12301 ttactccatt cctcaataga tttaaaggat ttgaattcgc ttttatcgac ttcccatttt
    12361 acatttaatc ccaggttaaa aacagtatgt accaatgaaa caaatgttgc catgtctgca
    12421 gattttacat catgatcgcg catgataaaa agtcctcctt ttcgtaatgt tcgttttatg
    12481 gaatttaaat aagtatccaa taaattatgc aggcaatgat gcaatccaat gtggcatgtt
    12541 attaaatcaa ggctttcatc ttttataata gaatcaggaa taggttgata gttgtcgagc
    12601 gaaaagaaag ttccaagttt actccattgc ccgcgttcca tcatatcggc aattgaattt
    12661 gttggtgcaa tgtcgtttga tataaatatg tttcctgaca gtttaatatg tttgcgcaac
    12721 tcgcttatgt agcgcccggt agagcctatt tccaaatatc catttataga ctttcgctcg
    12781 ccaagcaatt gcaaaacttg attcgccatt tctttttttt gcttttttaa tgccggaagt
    12841 gcataagtaa gttccgacag gaaagttttt attttgggca atccttgttg cactgcttta
    12901 taaatttcct cgtccgaatt tttttctttt gctgttgctg caataagttg atgaaattta
    12961 tcttcgggat acaaatgata aatcacctgc agaaaacgat aaaaatcatc gctccatttt
    13021 ggattcgaaa acactgcttt aaattctgat tttgtattgt ccatttatta tttttctgtt
    13081 ttacgatagt atgtatccca taatatattg cgaaatttat aatccggatc gagttgtgat
    13141 tttatttcga aaagtttttt tgcgtttgga tatgccgcat gaaattgttt ggatgttgca
    13201 tgtgcctgat aaggtaaata gtaggagcct tttacagata acacagcatc aatcaattcg
    13261 catgtccata cacctacttt gttttttgat gcttcatcgg tattttgttt ataccagatc
    13321 acaaatgcaa aaacttcttc gtgcgcccat gccaacaacg aaccgctatc gggcaatgca
    13381 tgccgaatag aaacattgat cacatttaca ttgtggcgca caaaaatttc tttcatccgc
    13441 gcagcaaata catcaaacat atttaccggc acaaaatact cctgcaatac ataggtgctt
    13501 tttttacgcg actcgggttc cagttctgct acatcatagc cggcttcgta attgcgccaa
    13561 tgcaccttgt tgcgggcaaa taaaatagga tcgtaaatgt attgcctgcg ccacttgcca
    13621 aatttacttt tgctgaatgc gccaataaaa tatcgctcca atggatacga tgccgcaagt
    13681 ggcattagcc gtgttggttc ggtggttttc ttttttgttt caacccaact tacggcacgc
    13741 accatattat atgctggcgg atatatatca ccattatgaa atatcacatc gctagtgttg
    13801 cgaacttttt cgaaaaagaa atttttatac tcaccaattt tcattgttgt attgattcgt
    13861 tctacagcaa cgttttccgt caactcaagt tcaacatttg caatcacagc tactgcattg
    13921 tagcaaccca cacaggcaaa aaataactcc gcattttcgg ttagcgaagc atgtttcatt
    13981 tcaccatttt gcaaaataac atctatagat tttaccgata aaataagcgg ccccaaaccg
    14041 atgtatctgc catggcaatt gacactcaag gaaccgccta ccgtaaagtt tgcataggtt
    14101 tgcataattt tgagactcaa attttctaca tcaatatatt gttgaatatc gcaccaacgt
    14161 atccccgctt ctactttaat agttttatgg tttggcgaaa agtcgattat tttattcaaa
    14221 ctgcgcatat caatgtgaat agtttgcgca cttgcagttt gcccgcccat actgaatctt
    14281 ccacctccaa tagaaacagc gttgttattt ttaataatat tttgcaactc ttctattgtg
    14341 cgtggtgttt caactttggc aacaataacc ggattgagtt gagtaacatc atttacaatt
    14401 cgttcgtttg ttttcaaatg tgggatagac tgtgcagcgc cattttcaaa atagtcggca
    14461 gcaagttttg gattaacacc atacttattt ttgttgagtg ttcctttttt taaacctaaa
    14521 aaataaaaca atagtaaagg acctgcaact ggaattgcaa taagcaaaag ccaccaagca
    14581 gataaattat tatcgtgtaa gcgttttgtt gctgttgcaa ttattgccca aaataaaagt
    14641 ggataaatta ttaaggttga tgaatatgaa ataaaaaatt ctaacgcatt gaatagcaca
    14701 tagaatgtag tccaaataaa tattgaaact gtccagtatg taccacgact tattctgcca
    14761 cgattagtga agagtaaata acttaccggg agtttgtctt taatggccat ttatgttttg
    14821 ctggtcttgt gcttaatatt aacacatggc gaatatgcaa aaaataatct aggggtagtt
    14881 ttcaaatatt gcgaatcagc aattcataca attgtattaa taaattgtaa aattcttata
    14941 catgttaggt tggcttttgt tgagagaatt tttaactggc ttattgtaaa attatttttc
    15001 actactaaga gcctcctata tttataaagc ttaaaagata actaacaaaa acaggaatca
    15061 ggctttgtac tcggcccatt cgctactctg tggcactttt atttccaaaa ccattattga
    15121 tattataata gcaagcacgc catcactaaa agcttctaat ctggttttat ttattaatta
    15181 ttttttaaaa cttttatgaa agtattatgt catttgtgtt tgacaggtat ccaaatttct
    15241 tcttccgaat cggggtcgtt gtgtttatac ttcgatccaa gtatttcgaa atgtgggcga
    15301 ttgtctaatt gataaataga atttggaatc catgcttcga atatgtagcg aaatgttttt
    15361 tctccctcct ctgcggcacc tttgtataga aacactgcgt acaaaccttg cggcaagatt
    15421 agtgtttgca ttccatccgg aacattagta aaatcagcga cttcaacagc ggcccatttt
    15481 tcgaaatttg catttatatc aaagtgcaaa aaattaaatt tcgtatcgta aatctgcact
    15541 gaaaataaat cgactccgtt agtatttttg atttcttctc tccttggcat aaaattcttc
    15601 cataactcaa ttgttctgtt actggccatg cacatttcta agtggttgcc gattaaatat
    15661 tttgctgata gatttttaat ttctggaatt atcattttgt aaaatcgtat tttcagttaa
    15721 aggagcaaca cacagaattt atttttatta aatcatatgc cctttgtggc atagttggta
    15781 actactattt cgtttatagc accacgcctg gtagcatttg aatttataat tcttcgtgca
    15841 ggtatgcgct caatattaaa accactataa agctcatcaa aaaaattatc gctggcatct
    15901 atgttctttg ggtccgaatt gctgagcata acctttacgc cttttttgtc gagtttttta
    15961 aatagtgatg caagttgctt ttgggcatcg tcaccaaaat cattttttgc atatgcttta
    16021 aaacttgatg ttttgctaat gggtctgtat ggtggatcga agtaaacaaa cgactttggt
    16081 tttatatcgt tcaacaaatt attaaaaacg gcttttttta tttcagcaat ttgtagaacg
    16141 cgatttgcgg caagcaaatt ctgctcatca caaatatttg ggtttgcata ttcaccggca
    16201 gggcaattaa attctccttt cgagtttacc cgatacagac cattgtagca ggttttgttt
    16261 aaaaaaataa attgtgctgc acgggcgaac catttatcag aataggaatt gtaatctgtt
    16321 ttgtttctat tcaaattgta ctttgttcgc tcatcgtaaa aaaaagattt ccttttttcg
    16381 ttatctaaac cggaatattt tttttgatat cggtgaagta attgcagcaa tgcgtcaaca
    16441 tctttttgca ctactttaaa agtcataata agctcgtcat ttatatcgta cagaaaggcg
    16501 ttttctattt gatatttttg aactacgtca aaaaatacag caccacttcc taaaaaaggt
    16561 tcgtaataat tttttatttt atggtcggtt aattcaggag ggtaaatgcc acgaaatttt
    16621 tccagcagtt gactttttcc tccggcccat tttaaaaatg gatgggcaac aatgttgttg
    16681 aaatttgttt gttccaaatg gaatatgatt taataacagc gctgttgttt ttttttgaat
    16741 cgtaattttg tattcgaagt ctgaaaattt ttattgaggc gaagttgaaa tgatagtgct
    16801 catggtttct tttgaaacgg aagcgtcttc tttattttga ttttacttat gcttgtttca
    16861 agcaatctgc gattattatt ttctcatttg tttcttcatt tatttcctct tttatttcct
    16921 catttatttc ctcttttatt cctcatttat ttcctcattt gtttcctcat ttatttcctc
    16981 atttgtttcc tcatttggta acaatccatt ttccgctttt agtacttcct attcgtttta
    17041 agcggccgct gtcgcgcaat atttgaatat gtctttcaat acttattttt gaaacattaa
    17101 tcttctgaac caattcgcca attgttacat tgctgttttg agtcagtttc ttagtacctc
    17161 aaccacttat acatttcttt ccaggtaacc tttttaccat acatcaaaat cccggtgcga
    17221 taaatttttg cgccaatcca tgtaaaagta ataaaggtaa ttatgagcag aaaaatgctc
    17281 aacaacattt gccacatagg aggatcgtat ggcacgcgca ccatcattac tattggcgat
    17341 gtaaatggaa taatgctgca ccacaggcca agcgcgctat cgggattcga aatgatggag
    17401 gctgcagcaa tgtatgcaaa tatgagcggg atggtaatgg gtagcataaa ctgttgcaca
    17461 tcggtttcgg catcaatggc agcacccaca gcagcaaaca tggatgcata caatagataa
    17521 ccaaacaaaa agtaaaaaat aaatagtccg attatttttc caaaattgaa tgacttaaat
    17581 tgctcaataa cctgcagcga ttgattggcg ttacctcccg gttgcatgtt catgcccgga
    17641 ttggccatgc ctgttttatt gccatcggag ttttgcgatt gcgctatttg ttgcgccatg
    17701 gcttccttgt cagtcatgag cgatgtaaca acagtgctca ccaacatggt tagcaatccc
    17761 cacaaaataa actgcgtaag agctactaat gcgatgccga taatttttcc catcatcagt
    17821 tgaaaaggtt ttaccgatga cataattact tcaactatgc ggctcgattt ttcttccatc
    17881 acgccacgca tcacctgagc gccataaata aatataaaca tgtaaatcaa tataccacca
    17941 ccaaatgcaa tggcggctgc tgcacctgcg cttacttccg aatcgccttt gccatcgccc
    18001 tgcaacgttt ttgtactgat tgaaatactt ttgtgaaggc tttttaataa caatggatcg
    18061 atgttgtttt ttacaagttg ttgttcgtca tatatttttt gtatatcatc ttctatgcgc
    18121 gaaacggtgc caaggcttac gagtttttcg gtgtacaatt gaaacacggt gctgtccttg
    18181 ttttcggccg actgataaat atgcagtaag ccatagaaat ttactttatt aaatttttcg
    18241 cgggcatttg aaaacgatgc tgtatcagca aaatatttta cactctccga acttttcagc
    18301 gtgcgggcat attctgcata ttgcgtttcg tccaccacct gaatatttga aatggttttg
    18361 tcgctatgtg tttgcatata aattactccg gcaaacaaac cgcccatcaa taatggacca
    18421 agaagtgtaa gcagaataaa ggattttttg cgcacgcgtg taatgtattc gcgttgtatg
    18481 atgagccaga ttttttccat cgttttgttt tttctttttt ggttatagta ctatgaatta
    18541 tttactgcct taataaatat gtcactcatt gtaggggtca cttcacgaaa caaatgtatt
    18601 tcgatattgt ctatcaatgc atgcaataat tgattgggtg tggcatcatc tattaatttt
    18661 acggtagcaa tattttcatc ctcatgtttt acattgctaa taagttcgaa gcccgcccaa
    18721 agagagtttg ccaatgagat attattaccg gcataatgta tttcgtaaat attcgatttg
    18781 aaatctttac gcacttcttt gacacttcca ttcaatacca ctttcgattt gtttatcaat
    18841 gcaatgtggg tgcacagttc ttccacactt tccattctat gtgttgacaa aactatcgac
    18901 acacctttgc ttttcatttc gagcaattca tcttttataa ggtttgcatt aatagggtcg
    18961 aatccggtga acggttcatc taaaatgagt aatcgtggtt tgtgaattac tgtagttata
    19021 aattgcactt tttgcgccat acctttacta agttcctcaa tctttttttt ccaccatcct
    19081 ttaagttcaa atttttcgaa ccaatagcgt aactctgtca tggcatcgct gcgcgacatg
    19141 cctttcagcc ttgccaggta gagtgcctgt tcgcccacct ccattttttt tgtaaagccc
    19201 a
//
LOCUS       DAORQA010000028        18231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_791_length_18231_cov_18.080931, whole genome shotgun sequence.
ACCESSION   DAORQA010000028 DAORQA010000000
VERSION     DAORQA010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 18231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18231
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_791_length_18231_cov_18.080931"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1927
                     /locus_tag="PK736_03615"
     CDS             272..1927
                     /locus_tag="PK736_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32512.1"
                     /translation="MKIIFNFLALIILSGNVTAQWTTDANNDTPTYLAPLSSNGMGNS
                     FTSDVDEQGNIFIGITISQFGGDIALQKVDSNGYQQWGTSGVVITSTTFNAQSSPFII
                     ADDIGGCYVAYDDNDFFNGAFIQHFDAQGIPQIPGKGKLILQNAYQTFGDISMLKQDN
                     FLYISLTAEKLSDKQVYLQKMDFNLNYVWDSTGVNLSLPGVDELQSKIISDGVGGMVV
                     LYNRYYSLFNYQVRAQRVDSAGNILWGPVGKFLNPANLLYSPSMKITADTNYHFYVVW
                     DAGANTNSDIYAQKLDTAGNFLWGVTELHVCDTVRVQNFPDVVADNNGGAYFVWSDRR
                     SSIPIDYCFAQYINSAGQKLWTKQGLAIDTLPYYNAIYPKLYYDSFGLNVFWTGTSSG
                     NRIYKQVLDSNGIQKCTAYGVPVTGLDHFSFDSDNIIPLANGDAVVACIDAFNFLYLK
                     YVPSGCSFTSVSVSESNFEHDFIIAPNPAVSNTLLIFDIKKQTEVTITIYDIAGKRMM
                     HSNLKADLGRNNIPLNLATWPKGFYTVEVMYGQKRQQKKLIVQ"
     gene            complement(2058..3347)
                     /locus_tag="PK736_03620"
     CDS             complement(2058..3347)
                     /locus_tag="PK736_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32513.1"
                     /translation="MKKKSLLFILMFANVLMFAQSPVYRVFVLNEGYFNWSTMTQVVS
                     PSVGAYNPVTKQYNTIATFNNASFASDVITDGNNLYVAASDGIYLFDKITNTQLDFEP
                     VIGIRKLSVWNNQLLCTRGDVGLPDNYFQVYDKTNLNLIYQLDKTTGPAYTTEGIVVA
                     NDTAYIALNNAFYFPNYVGKIGKVYLPSQTYTNEIDLGADGLNPDYLTYDNNTNKVIT
                     LNNLDFTNASVSSYSNSGATTTTKLNVGSGCASSVYANAGGDIFYQVSGESDLKKFNI
                     ATNANNGSLQINNYVYGMGYDATNDLIYAGNTDYVSFGKVYVYPSNSIIPTDSFNVSV
                     SPGTIFVDYSSATSMSENNTNSFNIFPNPANEIITVSNLNNASLITIRDVAGKNIIQQ
                     KVNNNTEQINISALLPGYYIVSLETSNGITKQPLLKR"
     gene            complement(3658..5976)
                     /locus_tag="PK736_03625"
     CDS             complement(3658..5976)
                     /locus_tag="PK736_03625"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRC32514.1"
                     /translation="MKKLIFISLFFIPFCVNAQTIRGRVTDASGLTPLQGVQIAFENG
                     ITNYNDYTQADGKFRLANIAAGRYKIIFTKDKYQTYTLEEVLSLSGKEIVLEVKMLRI
                     ASPLQIIEVSAVPLVLGNLNANRISVEQTQRYATVNDDVARAATYLPGVMAVNDGTNH
                     ISVNGHSPNDIGWYVNELMVINPNHLANAGTISDRPATAGGGISMLSSNMLSHTNFYN
                     GIFDIQFSNSLGGAFSNYLRNGNNEKFEYAIQGGLVGVDAAIEGPINKKSGASFLVNY
                     RYSFTGILAAMGVTFGGEDIRFQDLSFNIHLPVKQNNDINIFAVAGANSTYFSVPKDS
                     AEWTSQKFLTNAGYYANTILAGASWLHAFKNNNYLKAVIGINMMSDERKSAPANKQLN
                     YNALYNNTSEKNYLPYHVFYNHSFGIKQNLRVGIIGRTGNAKQEQQSAIDKENIAVEI
                     YNRNYTLQQHNPYIDYKLQASKKFAIELAVKMSMVNDASKAQPNYLGKIIFKINEANQ
                     LQYAANMTSQLIEKFVSQNMESQRNDFQPSFNNSLNHSIIFAHLFKNNALLKTELYYQ
                     QLNNIAVSAGIKNSFSTLNNFETSTTQPFVNTGTGTNYGVNINYMHPLSNHFYYMISV
                     SVFDAKYKGSDNIERHTRYANNHSTNFAGGYEWKNKKANKIFGISAHALYAGGMRYTP
                     IDIDQSELAQQTIVYDIAAFSLQQKDYFRTDLRFTYRVNRTHHNFLFALDIQNLAGNM
                     NTDYFYYDIYLKKVAEKKQISTVPIMSVKYSF"
     gene            complement(5973..6773)
                     /locus_tag="PK736_03630"
     CDS             complement(5973..6773)
                     /locus_tag="PK736_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32515.1"
                     /translation="MKFALNIKPSSILRAFTFVFAGFAVAGYILSFVSPETNKFINFH
                     FLQKLALYCLISTATFNHFLSTKKYLVVHLFIMMLGSALLLQINIFSALTSLLSVALL
                     FTKIPFTDKVKIHFHYLVVFAVSILSNQYSFMEHGWVLLLPVLFYASVWPVINDTGRP
                     IQKYTVILLMLAAIGVFAMGSLIPPFQILEALPFIIIWFFGVLYRLNQYFSDDNKNNN
                     VHLLEKIPSNIVLLNAAITAGFAGWYYGVFIFLLMPFSFVLFKTFEEQ"
     gene            complement(6875..7747)
                     /locus_tag="PK736_03635"
     CDS             complement(6875..7747)
                     /locus_tag="PK736_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRC32516.1"
                     /translation="MNENEVKALISLLDDDDNEVVTIVETQILNLGRAVIPVLEDAWS
                     QSFDPLKQQRIISIIHKLQHDDLLSSLEEWKKNPDDLLEGAIIVARHQYPDLEEDKIQ
                     SQINQIKRDIWLEINDNLTAYEKVKVINRVLFDMHQFKGNTANYHLPGNSYINIVLET
                     KKGNPLLLSLLYIQLAKQLDLPIYGVNLPEHFILAYVDEMNILREAFNDSSTRILFYI
                     NPFSRGVVFGRSEIDQFLDKLKMEHEKAYYEPCSNIDMISRLIRNLIFSYQKIGDTEK
                     ENELEEMLKVLSTK"
     gene            7909..8196
                     /locus_tag="PK736_03640"
     CDS             7909..8196
                     /locus_tag="PK736_03640"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HRC32517.1"
                     /translation="MQSEENNIKNLIGVVITQNKLKSKLNEVRIREAYEKVMGHTINI
                     RTTEVKLYGTKLSIAVNSAPLKAEMTFSKDKIIALLNAELGDTIIEDIWIK"
     gene            8248..9906
                     /locus_tag="PK736_03645"
     CDS             8248..9906
                     /locus_tag="PK736_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HRC32518.1"
                     /translation="MADVKHPKGLPFLFFSEMWERFGYYLMIGIFTLYLKDVKDGFAM
                     TEAESADLYGTFIALVFLTPFLGGLLADRYFGYRKSIIFGGLLMGVGYCMMGIHSLPM
                     LYLAMTLVIFGNGFFKPNISTLLGNLYSTEENKHLKDVGYNIFYMGINIGAFICNFFG
                     AALYLTLGWSYAFLAAGIGMFIGVIVFLVGTKHYKDFDIMKGTQPGDMSMGRISLLIL
                     LPSVVFGVLGWMIKGITNDANPGGNIFGSDSTDAFVFACLPVIYFYSSLYVKADAHEK
                     KPVGALLAIFAVVILFWAVFKLNGSALNTWADRYTDRQITGTAQSVFNSLKLSKDITY
                     KLDTVDKYDAAFRLQKVNGKVEKEYNYPIYFRNVSSNKLPAEGEKVSVWATNLSQSIN
                     PGWVILLTPLVVAFFSFLRIRKKEPTTPTKIALGLFISALSVLVMVAAVKAGSNGSEK
                     VAVWWLIANYGVITIGELCLSPMGLSVVSKLSPPRITSLMMGGWFLATSIGNKLSGVL
                     ASLWDTYDDKTNFFWLNFSLLIVATIFLLAMLKWLNGIFKEYVK"
     gene            complement(10040..10174)
                     /locus_tag="PK736_03650"
                     /pseudo
     CDS             complement(10040..10174)
                     /locus_tag="PK736_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973924.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
     gene            complement(10328..10648)
                     /locus_tag="PK736_03655"
     CDS             complement(10328..10648)
                     /locus_tag="PK736_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRC32519.1"
                     /translation="MNDKQAKADKEICVQLAAIINSQLRDTENKIWHAHPVWFLDGNP
                     IVGYSKQKRGIRLMFWSGADFDEKDLSIRGQKFKDASLFYNSVSEIVVKDLKRWLQKS
                     RAIQ"
     gene            complement(10950..11099)
                     /locus_tag="PK736_03660"
     CDS             complement(10950..11099)
                     /locus_tag="PK736_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32520.1"
                     /translation="MDLTIWGINSVFDICVIASKTLDGEINAKEKNYTMAISLLQEAV
                     AKEDA"
     gene            complement(11074..11220)
                     /locus_tag="PK736_03665"
     CDS             complement(11074..11220)
                     /locus_tag="PK736_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32521.1"
                     /translation="MIYPSVIWHYAQGMAMLLQNKIPDANKHLAEMKALMTDTTNGFN
                     NLGN"
     gene            complement(11376..12884)
                     /locus_tag="PK736_03670"
     CDS             complement(11376..12884)
                     /locus_tag="PK736_03670"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRC32522.1"
                     /translation="MKKIFIFFLSVIACSAYGQSVGINNPTPAASALLDLTSASKGLL
                     IPRVALTSTNMAGPVPGPVASLLVYNTATAGVAPNNVLPGFYFWDGVRWIAMSGGTGG
                     RDWSLTGNSGTVVGTHFIGTTDAEPLMFKVNNQQAGYLGNSSPWVTAFGYQALTSTTG
                     SFNTAIGYQALFSNTSGTENVGIGKEALRSNISGFENVAIGSNALFDNTTGNNNTAIG
                     DNALTNNINSHGNIAISTSAMANNTSASYNIAMGHAALFSQSFANGGTTYHAHNIAIG
                     YTALYNTNPANGTNGSSGKENVALGHTALYENITGFRNTGIGTAANVTTGALSNATAL
                     GYLAFASASNYVRIGNTAVTAIFGAVGFNTSDGRFKTNVKENVPGLDFIMRLRPVTYH
                     FEKLAYSRHLGETRDSINESELQRQDALGKVSTGFIAQEVEQLSDSLGYDFDGLYKPQ
                     NDKDSYGIAYQQFVVPLIKAVQEQQKIIAEDRERITRLEKQNAELLKLVNKK"
     gene            complement(13048..13230)
                     /locus_tag="PK736_03675"
     CDS             complement(13048..13230)
                     /locus_tag="PK736_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRC32523.1"
                     /translation="MLKIILIIIFLILNKQLTLNISYETVHSHIRKIYQKLQVNSVGE
                     AVSKTISKNILRMLFF"
     gene            13240..13530
                     /locus_tag="PK736_03680"
     CDS             13240..13530
                     /locus_tag="PK736_03680"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRC32524.1"
                     /translation="MTSQEILEVIKSNKKLFEQNYGVLNIGIFGSYARGEANEASDID
                     VLVELREPKYKYLYGLKSFLENRLHKNVDITRKGNHLRQNFLNSIEKEILYA"
     gene            13523..13885
                     /locus_tag="PK736_03685"
     CDS             13523..13885
                     /locus_tag="PK736_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRC32525.1"
                     /translation="MHDTIINERLHNILESISLIEERMLDITTPSDFRSTKEGLKTLD
                     SIAMRLQHIAENIKKIDGFQPDFFETKIIMDPQPIINFRDFISHHYERTDVEIIYDIC
                     LNDIQELKVKINNYWKFS"
     gene            complement(13905..14555)
                     /locus_tag="PK736_03690"
     CDS             complement(13905..14555)
                     /locus_tag="PK736_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC32526.1"
                     /translation="MQVIIFDDNINVRESIGLLLSTTDNMQLVAAFDSCKNMLADIEN
                     INPDIVLMDIDIPLITGIDAVKLLRTKYQALPVLMLTGFEDDDKVFASLCAGANGYIL
                     KNANMQSLIQYINEVYHGGAPMTPVIARKVLTQFAKIQPVKQPDENYNLSRREKDVLQ
                     LLVKGKSYKMIADELNISYETVHSHIRKIYQKLQVNSVGEAVSKTISKNILRTYLF"
     gene            complement(14646..17711)
                     /locus_tag="PK736_03695"
     CDS             complement(14646..17711)
                     /locus_tag="PK736_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRC32527.1"
                     /translation="MRKKILSLLLFMLVSNASSSQQKNITLRRITSQQGLSDNQVTCT
                     IHDKQGFIWIGTKDGLNRYDGQDFYVFKHNEKDINSLCGNIISCLEIDNDSLLWIGSA
                     SSGMCSYDFRTGKFKTYNKSNSELNVNNINDITFDKNRNCLWIAQNNAGLQLFDLKTK
                     ATDKNKKLISTNTYYDVLVNDTIPYFAGIIESLKRLGNIGKFRTQVSDTAMTINKIFL
                     GSDNHLWCGAWDNGLHQFDLNGKRLATWFFDRSLKLKLSGDEILCLAEDENKVLWCGT
                     KSSGIRFFDLKTKSFTNDIKFSNPVTSRINSLYRDIRNRIWIASETGLYVYDPLQNQF
                     EITKLPFPPGTTSCKANDRIILKSGREFIVTQCGFFYRNSNDENYLHKDFYYRDERQE
                     LTSIFLDEKNIIYIGTNRSVFTLDTVTLELVFPKVSYAASKHQSFFMGGSRANSITQI
                     RHHEHSLIAVSLYGNYIKLFDTQHKNAFINLKDTNVEGTFVDNLSRKIFSDSKNNFWV
                     CGATHGINQVLIPQRISFDEFPFADSIVRVIHSQSKTWNRPDTKNVKSVNNVFDMTEN
                     TDGSYWVTTQGQGLLKFFPENDSVTFVSYANEIKSLQGLAKDKNDNLWMVSSTGLLHY
                     NVKGNRYKLFDMQSGISENISGYFFRNDNSTIQDEMSVGFDGGFISFYPSQIISNHEK
                     PFVSISRLWIMDAPSDSLLFDQLKLNYNRNFLKFNVAANSFSDNGQTICLYMLDGIDD
                     TWRNNQTNPLITYTNLPHGNFTLKVKAVNSDGIESDVYELPFIITPPFYFTWWFYSLL
                     LIVITKSVYVLYRYRIRQILKLQEVRNKIARDLHDDIGSTLGSIHLYSQIANAKLKGD
                     KSDEIKSILEKIENSSVEIIDKTSDVVWAAKASNDTLKNLILRMEGYSASILGATNIQ
                     FNIEYDERITEMKLEMTERKNIFLIYKEAIHNIIKYANCTEVNISIKKNADKLYVSIS
                     DNGSGFISDELKSYNGNGIKNMKSRASEIKGSIEITSFPGKGTIIELTV"
BASE COUNT         5527 a         3271 c         3328 g         6105 t
ORIGIN      
        1 cctaaattat gcagtagaaa gctagggaca agccgagcta cctgcattag ctgcgttaat
       61 cccgattttg aaaatcacct tttggaaaaa tgaaataaag ccaaaagagt tgactttatt
      121 aattgttatg cgattaaaaa tttcaactgc ctaattcggg tttaaacata agttgaatat
      181 aatttacaaa tcaaattaag atgcctttaa cttttgtttt gttaagtaag aagtaaattt
      241 gctacattat taattttgaa tgcggataac aatgaaaatt atttttaact ttttggccct
      301 gataattctt tcgggcaatg tgacggcaca atggactacc gatgccaata acgacactcc
      361 tacttatttg gctccattgt cctcaaacgg catgggtaat tcgtttacaa gcgatgttga
      421 tgaacaaggc aatattttta ttggtattac tatatcacaa tttgggggcg atattgcttt
      481 gcagaaagtt gattccaatg gttatcaaca gtggggaaca agtggagttg taattacttc
      541 gacaacattt aatgcacaat cgtctccttt cattattgcc gatgatattg gaggttgcta
      601 tgttgcatat gatgataatg atttttttaa cggtgcattt attcaacatt ttgatgcaca
      661 gggtataccg caaattccgg gcaaaggtaa actcattttg cagaatgctt atcaaacatt
      721 tggtgacatt tctatgttga aacaagataa ctttttgtac ataagtctaa cagccgaaaa
      781 attgagtgat aagcaagtct atttgcaaaa aatggatttc aatttaaatt acgtttggga
      841 tagcacagga gtaaatttat cgttgccggg tgttgatgaa cttcaatcga aaataataag
      901 cgatggagtg ggaggaatgg ttgttttata caacaggtat tatagtttat tcaattatca
      961 agtgcgcgcg cagcgtgttg atagtgcagg aaatattttg tggggccctg taggtaaatt
     1021 tctaaatcct gccaacctat tatattctcc cagtatgaaa atcactgctg ataccaatta
     1081 tcacttttat gttgtgtggg atgcgggtgc aaataccaac tccgacattt atgcacagaa
     1141 acttgatact gccggtaatt ttttgtgggg tgttactgag ttacatgttt gcgatacggt
     1201 gcgtgttcaa aattttcctg atgttgttgc cgataacaat ggcggtgctt attttgtttg
     1261 gagcgatagg cgttcatcta ttcctattga ttattgtttt gcccaatata taaattcagc
     1321 agggcaaaag ctttggacaa agcaaggttt ggctattgat acattgccat actataatgc
     1381 catttatcct aaattgtatt acgattcttt cggattaaat gtcttttgga ctggtacttc
     1441 atccggcaat cgcatctata agcaagtttt agacagcaat ggaattcaaa agtgtactgc
     1501 ttatggtgtg cctgttaccg gccttgacca tttttcattc gatagcgata atattattcc
     1561 gcttgcaaat ggcgatgcgg ttgtagcttg tatagatgcg tttaactttt tgtatttaaa
     1621 atatgtgcct tcaggatgtt cattcacttc ggttagtgta agtgaatcta actttgaaca
     1681 tgattttatt atcgccccaa acccagccgt ttctaatact ttacttatat ttgatattaa
     1741 aaaacaaaca gaagtaacaa taaccatata cgatattgcg ggtaaaagga tgatgcattc
     1801 aaacttgaag gctgatttag gaagaaataa tattccgctt aatttagcaa catggcccaa
     1861 aggattttat actgttgaag ttatgtatgg ccaaaagcgc caacagaaaa agttgatagt
     1921 acaatagttg ttgattttgt attggaagtt tgtgtcatat cagcatggca caaccatatt
     1981 ttttaaatgt taggtgtgta ccgaaaagaa aaagcctcga aatatttcga ggctttttaa
     2041 ttagaataaa ttcgtgatta tcttttaagt aaaggttgtt tggtaatccc attcgaggtt
     2101 tctaatgaaa caatgtaata acccggcaac aaagcagaaa tgtttatttg ctcggtattg
     2161 ttattcactt tttgttgtat tatatttttt ccggcaacat cacgtatagt tattagtgaa
     2221 gcattattta aattgcttac agtaataatt tcatttgccg gatttgggaa gatgttgaaa
     2281 ctgtttgtat tgttttcact catgctggtt gccgaagagt aatcaacaaa aatagtacct
     2341 ggagaaacag atacattaaa tgaatcggtt ggtataatac tgtttgaagg atacacgtat
     2401 actttgccaa agctaacata atcagtattt ccggcataaa tcaaatcgtt ggtagcatca
     2461 taacccatac catatacgta gttatttatt tggagtgaac cattatttgc atttgtggca
     2521 atattgaatt ttttcaaatc actttcgccc gatacctgat aaaaaatatc gcctccggca
     2581 ttagcatata cagacgaagc acagcccgaa ccaacattta attttgttgt tgtagttgca
     2641 cccgagttag aataagaact tactgatgca tttgtaaaat caagattgtt taatgtgatt
     2701 actttatttg tattgttgtc gtaagttaaa taatcaggat tgaggccgtc agcacccagg
     2761 tctatttcgt tcgtatatgt ttgcgatggt aaatacactt tgccaatttt gccaacatag
     2821 tttggaaagt aaaatgcatt gttcaaagca atgtatgctg tatcatttgc aactactatg
     2881 ccttccgtgg tataagcagg ccctgttgtt ttgtcgagtt ggtatatcaa attcaaatta
     2941 gttttgtcat aaacttgaaa gtaattgtcg ggcaatccaa catcgccacg ggtgcatagc
     3001 aattgattat tccaaacact caatttgcgt atgccaatca caggttcaaa atcgagttga
     3061 gtattggtta ttttatcgaa caaataaata ccatcgctgg cggctacata aagattattg
     3121 ccatcggtga ttacatcact agcaaacgat gcattgttga aagttgcaat ggtgttgtac
     3181 tgttttgtta cggggttata agcacctacc gaaggtgaaa caacctgcgt catggtgctc
     3241 cagttgaagt aaccttcgtt gagcacaaat acacgataca cgggtgattg agcaaacatg
     3301 agaacgtttg caaacattaa aataaaaagt aaagattttt ttttcataat attatgtgtg
     3361 ttaaattatt taaaattgaa aagataatgc aacacaacat cactattata taatagcggc
     3421 agagatgttg acattaactt atattcgcga agttaatcag tcaaagttac gaagtatgta
     3481 tctggctcaa agagaaaatc tcttcataca gttgcgcgtc agcccacgaa ttgcacgtga
     3541 ttccattctg ttattgtatt ggcttcgtaa cctttttatg aaagaactgg cgcaaatgta
     3601 aaaaagttat tgttgtaaca acaactatga attaataaac ttgccaaccg gaaaacatta
     3661 aaagctatac ttcaccgaca taattggaac tgtagatatt tgttttttct cggctacttt
     3721 tttcaaatat atatcatagt aaaaataatc ggtattcata tttcctgcaa ggttttgaat
     3781 atcgagtgca aataaaaaat tgtggtgcgt gcgatttaca cgatatgtaa atcgcaaatc
     3841 ggtgcggaag taatcttttt gctgtaaact gaatgctgca atatcataca ctattgtttg
     3901 ttgtgcaagt tccgattgat ctatatcgat aggagtatac cgcatgccgc ccgcatacaa
     3961 agcatgcgca cttatgccaa atattttatt ggctttttta tttttccatt cataaccacc
     4021 ggcaaaattt gttgaatgat tgtttgcata acgggtatgc cgttcaatat tatcgctgcc
     4081 tttatacttt gcatcgaata cagaaaccga tatcatataa tagaagtgat tgctaagcgg
     4141 gtgcatataa ttaatattta ctccgtagtt ggttcctgtt cctgtattta caaaaggctg
     4201 agttgttgaa gtttcaaaat tattcaatgt ggaaaagcta ttttttattc ccgcactcac
     4261 tgcaatattg tttagttgct gatagtataa ttcggttttt agcaatgcat tattcttgaa
     4321 taaatgtgca aaaattatag aatgattgag gctattgttg aacgaaggct ggaaatcatt
     4381 tctttgtgat tccatatttt gtgaaacaaa tttttcgatc aattggcttg tcatatttgc
     4441 tgcgtactga agttgatttg cttcgttaat tttgaaaatt attttcccta aataattcgg
     4501 ttgcgctttg ctggcatcat tcaccatgct catttttacc gctaattcga tagcgaattt
     4561 tttacttgcc tgcaatttgt aatcaatgta agggttatgt tgttgcagcg tgtaatttct
     4621 gttataaatt tctacagcta tgttttcctt atcgatggca gattgttgct cttgctttgc
     4681 attacctgtt ctgcctataa ttccaacgcg taaattttgc tttatgccaa agctatgatt
     4741 ataaaaaacg tgataaggca aataattttt ttcggaggta ttattataca aagcattata
     4801 gttgagttgt ttattggcag gagcactttt gcgttcatcg ctcatcatat taataccgat
     4861 aacagctttc agataattat tgtttttaaa agcgtgcaac cacgatgcac ccgccagaat
     4921 ggtgttggca taatatcctg cattggtgag gaacttttgc gaagtccatt cagcagaatc
     4981 ttttggaaca ctaaagtatg tactgtttgc accggcaaca gcaaaaatat ttatatcgtt
     5041 gttttgtttt accggcaagt gaatattgaa cgatagatcc tgaaagcgaa tatcctcgcc
     5101 accaaatgtt actcccatag ccgcaagtat tccggtaaaa gaataccggt aatttaccaa
     5161 aaacgatgca ccactttttt tattaatagg accttctatg gcggcatcaa cacctaccag
     5221 gccgccttgt attgcgtact caaatttttc gttattgccg tttcgcaggt aattgctaaa
     5281 agcaccacca agtgagttcg aaaactgtat gtcgaaaatg ccattataaa aattggtgtg
     5341 cgaaagcata ttgctgctaa gcatacttat accaccacct gcagttgcgg gtcggtcgct
     5401 aatggtgcca gcattggcaa ggtgattggg atttattacc atgagttcgt ttacatacca
     5461 gcctatatcg tttggcgagt gcccgtttac agaaatatga ttggtgccat cgttcactgc
     5521 cataacgccc ggcaggtatg ttgctgctcg cgctacatca tcgttcactg tggcgtagcg
     5581 ctgtgtttgt tctacactaa tgcggttcgc attgaggttg ccaagtacaa gcggcacagc
     5641 agatacttct attatttgta acggtgacgc tatacgaagc attttaactt caagcactat
     5701 ttcttttcca ctaagcgaca acacttcttc caatgtataa gtttggtatt tgtcttttgt
     5761 aaaaattatt ttgtaacgcc ctgcagcaat attggctaat cgaaatttgc catcggcctg
     5821 tgtgtaatca ttataatttg taatgccatt ttcaaatgca atttgtactc cctgcaaagg
     5881 cgttaatccc gatgcatccg ttacccggcc acgaatggtt tgtgcattaa cgcaaaacgg
     5941 aataaaaaaa agcgaaataa aaatgagttt tttcattgtt cttcaaatgt tttaaataaa
     6001 acaaaactaa aaggcattag caaaaatata aacacaccat aataccatcc tgcaaaacct
     6061 gcagttatag cagcatttag caacacaata tttgaaggaa ttttttcaag taaatgtaca
     6121 ttgttgtttt tattgtcatc ggaaaaatat tgattgaggc gatacaaaac cccaaaaaac
     6181 cagataataa taaacggcaa tgcttccaga atttggaatg gcggtattaa tgatcccatt
     6241 gcaaaaacac ctatggcagc aagcattagc agaataacag tatatttttg aattggtctg
     6301 ccggtatcat ttattacggg ccataccgaa gcgtaaaaca ataccggtag cagtaaaacc
     6361 cagccgtgtt ccatgaagga atattgattg gatagaatac tcacagcaaa tacaacaagg
     6421 taatggaagt gtatttttac tttatcggtg aaggggattt tagtaaataa caatgccacc
     6481 gaaagcagag aagtgagagc cgaaaaaata tttatttgta aaagcaaagc gctgcctagc
     6541 atcataataa aaagatgaac gactaaatat ttttttgtgg ataaaaagtg attgaatgtt
     6601 gctgtggaaa taaggcaata caacgctaac ttttgaagaa aatggaagtt aataaattta
     6661 ttggtttcgg gcgatacaaa actaagaatg tatccggcaa ctgcgaatcc ggcaaacaca
     6721 aaagtaaatg cacgcaagat ggaggaaggt tttatattta aggcaaattt catttgatgt
     6781 attttacgta aaaagtcgtc acttatttat tggtgcatga ttaacacctt tttagtttca
     6841 atgtttcatt agtggtaggt atatccactt gtatttattt tgtagaaagc actttgagca
     6901 tttcttcaag ttcattttct ttttcggtat cgccaatttt ttggtacgaa aaaataagat
     6961 tccgaatcag tcgcgaaatc atatctatat tcgaacaagg ttcatagtat gctttttcgt
     7021 gttccatttt aagtttatcc aaaaactgat ctatttcgct acggccaaat actactccac
     7081 ggctaaatgg attaatgtaa aacaaaattc ttgtagagct atcgttaaat gcctcgcgaa
     7141 gaatattcat ttcgtcaacg taagcaagta taaaatgctc gggaagattc acaccatata
     7201 ttggcaggtc gagttgtttt gccaactgta tgtataataa agaaagaagt aacggattac
     7261 cttttttcgt ttcgagcaca atgttgatgt atgaatttcc tggtaggtga taatttgccg
     7321 tattgccttt aaactgatgc atatcgaaaa gcacacggtt aataactttt actttttcgt
     7381 atgccgtaag gttatcgttt atctccagcc aaatatcacg ctttatctga tttatctgcg
     7441 attgaatttt atcttcttcc aaatcaggat attgatgccg ggctactata atggcccctt
     7501 cgagcaagtc atcgggattc tttttccatt cctcgagact gctgagcaag tcatcgtgct
     7561 gcaatttgtg aataatgcta atgatgcgtt gttgcttcaa cgggtcgaac gactggctcc
     7621 atgcatcttc gagcaccgga ataacagcac gccctaaatt gagtatttga gtttccacaa
     7681 tggtaaccac ttcgttatca tcatcgtcaa gaaggctgat aagtgctttt acttcgtttt
     7741 cgttcataca gcgttgcgct atttttttta caatagtaca acgccaagtt taaatattgg
     7801 taattatact tttcttattt tgaacaatct atgttaaaaa ttatgcataa ctattttgat
     7861 ttgaagtatg acactaaatt gtttcatttg taagaattaa taagcaacat gcaatcggag
     7921 gaaaataata tcaagaattt gataggagta gtaatcactc aaaataaatt aaaatcgaaa
     7981 ctcaatgaag tgagaattcg tgaagcctac gaaaaagtaa tggggcacac tatcaatata
     8041 agaactaccg aagtgaaact atatggtacc aaattgagca ttgctgtaaa ttcggcaccg
     8101 cttaaagcag aaatgacttt tagtaaagat aaaattattg cattactcaa tgccgagctt
     8161 ggcgatacta ttatcgaaga catttggata aagtaatttg caaatgtttt aaatataaaa
     8221 taaaatctaa caaacttttt aaataatatg gcagatgtaa aacaccctaa agggttaccg
     8281 tttttatttt ttagcgaaat gtgggaacgt tttggctact acctcatgat aggaattttt
     8341 acgctttatc tcaaagatgt gaaagatggt ttcgccatga ccgaagctga aagtgctgac
     8401 ttatacggaa cttttattgc cctggttttt cttactccat tccttggcgg cctgcttgcc
     8461 gacaggtatt tcggctatcg taagtccatc atctttggtg gtttacttat gggtgtgggt
     8521 tattgtatga tgggtataca ttcactgcct atgttgtatt tggcaatgac gctggtcata
     8581 ttcggaaatg gattttttaa accaaacatt tcaacactgc tgggcaattt atattcgacc
     8641 gaagaaaaca aacatctcaa agatgtaggg tataatattt tttacatggg gataaatatt
     8701 ggtgccttta tatgcaattt tttcggtgcc gcactttacc tcacactggg ctggagttac
     8761 gcttttcttg cagccggtat aggcatgttt attggcgtaa tcgtttttct tgtaggcaca
     8821 aaacactaca aagatttcga tattatgaaa ggcactcaac ccggtgatat gtctatggga
     8881 cgaatatcat tgctcatatt attgccatct gttgtgtttg gcgttttggg atggatgata
     8941 aaaggaataa caaatgatgc caatccgggc ggtaatattt ttggttcgga tagtaccgat
     9001 gcatttgtat ttgcctgcct gccggtgata tatttttatt cttctttata cgttaaggca
     9061 gatgcccacg aaaaaaaacc tgtgggtgct ttattagcaa tatttgctgt agtgattttg
     9121 ttttgggccg tatttaaact caatggttcg gcactcaata cctgggccga tagatatacc
     9181 gaccgtcaga taaccggtac agcacaatct gtttttaatt ctctaaagct atcaaaagac
     9241 attacataca aactcgatac ggttgataaa tacgatgcag catttcgttt gcaaaaagta
     9301 aatggcaaag ttgaaaagga atacaattat ccaatttact ttcgcaatgt gtcgtcaaat
     9361 aaattaccgg ccgaaggtga aaaagtaagt gtatgggcaa ccaatttaag tcagtcgata
     9421 aacccaggtt gggtaatatt actcactccg cttgtggtgg cattcttttc atttttgcgc
     9481 atcagaaaaa aagaacctac cacaccaaca aaaatagctt taggtttatt catctctgca
     9541 ctgtctgttt tagtaatggt ggctgctgta aaagccggaa gcaatggcag cgaaaaagtt
     9601 gctgtttggt ggcttattgc aaattatggt gttatcacca ttggcgaatt atgtctgagt
     9661 ccaatgggct tatcagttgt ttctaaactc agtccaccac gaattacttc gcttatgatg
     9721 ggcggatggt ttcttgccac ttctataggt aacaaactaa gcggagtgct tgcaagcctc
     9781 tgggatactt acgatgataa aacaaatttc ttctggctaa atttttcgtt actcatagtt
     9841 gccacaatat ttttattagc catgttgaaa tggttaaatg gcatttttaa ggagtatgtg
     9901 aagtaagctt aaatgtaatt agccccgaca cttcgattgg gataccgact gaagtgttgg
     9961 cattaaaatt caaaaagccc aataaatatt tttattgggc tttgtatttt gtgcataagt
    10021 agcctcactt cttcaatctt gtcagccact caatcacttg atgtaattca acttttgtcc
    10081 tgcctttatc ctccactttt gtgatgtagt gcggataaac ctttgcaaaa gtcatttttg
    10141 caattcgtac atcgtgttca ggtattgttt tcatactatt ttatattgaa gatattttgg
    10201 tttccggaat tgtacttctt caaatcacta aaaatcatgt caatcaatca tggtaatccc
    10261 gaaaatcaaa ttaatccagg tttaattttt ctaattgtcc ttttcttttt actatgtttt
    10321 tgtagtctca ttgaattgct cttgattttt gcagccaacg cttcaaatct tttaccacaa
    10381 tttctgatac gctattgtaa aatagagagg cgtccttaaa tttttgtcct cttatgctta
    10441 aatccttctc atcaaaatct gccccgctcc aaaacatcag cctgattccc cgtttctgtt
    10501 tgctgtaacc aacaattgga ttgccatcta aaaaccaaac agggtgagca tgccagattt
    10561 tattttctgt atcgcgcagt tggctgttta taatagcagc aagctgcacg caaatttctt
    10621 tgtcggcttt agcctgtttg tcatttatac tttaatttct ttattcatag ttctgccatt
    10681 tatatgttac aatattcgtg acgatgtgat tttaatttca gcatatttcc atgtttcatc
    10741 aaaacggatt tttgtttcat catatttctt tttgtcgcca agtttttcgt aagcattcat
    10801 cactcctcgc aattcccaac cgttttcgcg gtaggttttt aaatcctcat cataaacttt
    10861 tatcgcatcc tgaaattttc ctgcttcaat taaaactgcc ccaaatgatg tcgaacagaa
    10921 aaaaaccagt catgcggttc gtcataattc tatgcatctt cttttgcaac ggcttcttgt
    10981 aaaagtgaaa ttgccattgt atagtttttc tcttttgcat taatttctcc atccaaagtt
    11041 ttggaagcaa tcacacatat atcaaacaca ctgttaattc cccaaattgt taaatccatt
    11101 agttgtgtcg gtcatgagcg ctttcatttc tgcaagatgt ttattcgcat caggaatttt
    11161 attttgcaat agcattgcca tgccttgtgc ataatgccaa atcacggatg gatatatcaa
    11221 atctttttcg ggttaagttg aatgaagaat atcgttccat aaaccaagct aggttgaatc
    11281 ctgtcgtgca acttcaaaaa atgaacgccc tgcttcggca tgattgaatt aaaagtaaaa
    11341 tacatgacgg ttctttcatt agaagttttc tgcatttatt ttttattcac aagcttcagc
    11401 agttctgcat tttgtttttc taagcgggta atgcgttcac ggtcttccgc aataattttt
    11461 tgttgttcct gcaccgcttt tataagcggt actacaaatt gctggtatgc gataccataa
    11521 gaatctttat cgttttgcgg tttgtaaagc ccgtcaaaat cataacccag cgaatcggat
    11581 aattgctcca cttcctgtgc aataaagcct gtgcttactt tgccaagtgc atcctggcgt
    11641 tgcagctcgc tttcattaat agaatcgcgt gtttcgccaa gatgccggct gtaagccagt
    11701 ttttcgaaat ggtaggtaac cgggcgaagc cgcatgataa aatcaagccc cggtacgttt
    11761 tctttcacat tggttttaaa tcggccatcg ctggtattaa aacctacagc gccaaaaata
    11821 gcggtaacag ctgtgttgcc tatgcgaaca taattgcttg cggaggcaaa agcaaggtag
    11881 cccagggctg tggcattgct aagtgcaccg gtggttacat tggcagcggt tccaatgcct
    11941 gtattacgga aaccggtaat attttcatac aatgcagtat gtcctaatgc aacattttcc
    12001 ttgccgcttg agccgttagt gccatttgca gggttagtgt tatataacgc tgtataacca
    12061 atagcgatat tatgtgcatg atacgtagtc ccgccattgg caaaactctg tgaaaataaa
    12121 gcagcatggc ccatagctat attgtagctt gctgaagtat tattcgccat agccgaagtg
    12181 ctgatggcaa tatttccatg actgttaata ttgttggtta aagcattatc tccaattgct
    12241 gtgttattat ttccggttgt attatcaaaa agagcattac tccctatcgc aacattttcg
    12301 aaaccggaaa tattactgcg cagcgcttcc ttaccgatgc caacattctc cgtgccactg
    12361 gtattggaaa aaagcgcctg atagccgatt gctgtattaa aagaacctgt agtggaagtc
    12421 aatgcctggt atccaaatgc agttacccac ggtgatgaat taccaaggta tcccgcttgt
    12481 tgattgttaa ccttaaacat gagcggctct gcatcagtag tgcctataaa gtgggttcca
    12541 acaacggtac cgctattgcc tgtgagcgac cagtccctgc cgcctgtgcc tccgctcata
    12601 gcaatccacc ttacaccatc ccaaaaataa aaaccgggca atacattatt tggagcaacc
    12661 cctgctgttg ccgtattata aactaagagt gaagctacag gaccgggaac cggacctgcc
    12721 atatttgtag aggttaaagc aacacgtgga ataagcaagc ctttgctggc gcttgttaaa
    12781 tcgagcagtg cgctggctgc aggtgtggga ttattaatgc ctacgctttg tccgtaagcg
    12841 ctgcatgcaa ttacagacaa aaagaaaata aatatttttt tcatatcaga tagtatgtgg
    12901 ttcatgtact ttttaatttt cgtaaaaaaa agacacattg aataatgtta acagttttaa
    12961 ccattcaaaa ttgaggattt tgtctgttca catcaatcac caattatggt gaatttggta
    13021 aatgcaggaa aataacaatt gaataattta aaagaaaagc atgcgcaaaa tattttttga
    13081 aatggttttg gatactgctt cacccacaga attcacctgc agcttttgat aaattttgcg
    13141 aatgtgcgaa tgcacagttt cgtagctgat gtttaatgtt aattgtttat ttaaaattaa
    13201 aaaaatgatt atcagaatta ttttcagcat ttttgcatca tgacaagtca ggaaatacta
    13261 gaggttataa aatcaaataa gaaattgttt gaacaaaatt atggtgtttt gaatattggc
    13321 atttttggtt cctatgcccg tggcgaagcc aatgaagcaa gtgatattga tgttttggta
    13381 gaacttcgtg aacctaagta taaatatttg tatgggttga aatcattcct tgaaaaccgg
    13441 ttgcataaaa acgtagatat tacaagaaag ggaaatcatc tccgtcagaa ttttttaaac
    13501 tcaattgaaa aggaaatttt gtatgcatga taccatcatc aacgagcgac ttcataatat
    13561 tttagaatcc atctcactga ttgaggaacg catgttggat attactactc catcagattt
    13621 caggtctaca aaggaaggat taaaaacatt ggattctatt gccatgcgct tgcagcatat
    13681 tgctgagaat ataaaaaaga ttgatggatt tcaacctgac ttttttgaaa caaaaattat
    13741 aatggatcca caaccgatta taaatttcag agattttatt tctcatcatt atgaaagaac
    13801 tgatgtggaa attatctatg acatttgttt gaatgatata caggagttaa aagtgaaaat
    13861 aaataattat tggaagtttt cataactcgc agactataca agtcctaaaa caaataagtc
    13921 ctcagtatat ttttcgaaat tgttttagat accgcttcac ccacagaatt tacctgcagc
    13981 ttttgataaa ttttgcgaat gtgcgaatgc acggtttcgt agctgatgtt taattcatct
    14041 gcaatcattt tatatgattt gcctttcacc aaaagctgaa gcacatcctt ctcgcgtctg
    14101 ctcaagttat agttttcatc gggttgctta acaggttgaa tttttgcaaa ttgagttaat
    14161 acttttcttg ctataacggg tgtcattgga gcaccaccat gatatacttc atttatgtat
    14221 tgaataagcg attgcatatt ggcattcttt aaaatataac cgttggctcc tgcacacagc
    14281 gaagcaaaaa ctttatcgtc atcttcaaaa cctgtcaaca ttaaaacagg caatgcctga
    14341 tatttagtac gtaataattt tacagcatca atgccggtaa tcaatggtat atcaatatcc
    14401 atcaatacaa tatcagggtt tatgttttca atatctgcca gcatattttt gcaggagtca
    14461 aaagcagcaa cgagttgcat attatcagta gttgacaaaa gcaaaccaat agactcgcgc
    14521 acgttaatat tatcatcaaa aataattacc tgcatcaatt ttttttcctc tgttatcatg
    14581 atgcaaaagt gagtgtaatt gttttcaata ccaataacca attatggtga agtgaataga
    14641 tggctttata cagttaattc aataatggtt cctttacccg gaaacgatgt gatttctata
    14701 ctacccttta tttcagaagc tcttgatttc atgttcttaa tgccgttgcc attatatgat
    14761 tttaattcgt ctgaaataaa accacttccg ttatctgaaa ttgaaacata taatttgtct
    14821 gcatttttct ttatgctaat gtttacttcc gtgcagttgg catatttaat aatgttgtga
    14881 atggcttctt tgtaaatcaa aaaaatattt ttccgttccg tcatttctaa tttcatttca
    14941 gtaatacgtt catcatattc aatattaaat tgaatgttag ttgctcctaa aatggaagct
    15001 gaatatcctt ccattctcaa tattaaattt ttcaaagtat catttgatgc cttggccgcc
    15061 caaaccacat cactggtttt atcaataatt tctaccgatg aattttctat tttttctaag
    15121 atagatttta tctcatctga tttatcacct tttaactttg cattggcaat ctgactgtaa
    15181 aggtgaatac tccctaatgt tgaaccaata tcatcgtgca aatcgcgggc aatcttattc
    15241 cgaacttcct gcaattttaa aatctgccgg attcgataac gatacaaaac gtacacagat
    15301 ttcgttatta ctattaaaag cagactgtaa aaccaccagg tgaaataaaa tggcggtgta
    15361 ataataaacg gcaattcata aacatcgctt tcaattccat cgctgttgac agccttaact
    15421 ttaagtgtaa aatttccatg cggcaaattg gtgtaggtaa tcagtggatt ggtttgatta
    15481 tttcgccagg tatcatcaat gccatcaagc atatacaagc atatggtttg accgttgtcg
    15541 gaaaagctat ttgcagcaac attgaatttc aaaaagtttc gattgtaatt caatttcaat
    15601 tgatcaaaca acaacgaatc agaaggcgca tccataatcc acaatcttga aatactgaca
    15661 aatggttttt cgtggtttga aataatctga gaaggataaa aggaaataaa accaccatcg
    15721 aaaccaacac tcatttcatc ttgtattgtt gaattgtcat ttcgaaaaaa atacccgctg
    15781 atattttcag aaatcccgct ttgcatgtca aacaacttgt aacggtttcc ttttacattg
    15841 taatgcaaca aaccagttga actaaccatc caaagattat catttttatc ttttgccaaa
    15901 ccctgcaacg acttaatttc atttgcataa gacacaaatg ttacagaatc attctcggga
    15961 aaaaacttca gcagcccttg tccctgtgtg gttacccagt aagagccgtc agtgttttct
    16021 gtcatgtcaa aaacattgtt cactgatttt acattttttg tatcaggtct attccaggtc
    16081 tttgattggg aatgaatcac ccgcacaata ctgtcggcaa aaggaaactc atcgaatgaa
    16141 atcctttgtg gaatcaaaac ctgattgata ccatgggtgg caccacaaac ccagaaattg
    16201 tttttcgaat cactgaaaat tttccgtgat aaattatcca caaaagttcc ttctacgttc
    16261 gtatccttta aattgataaa tgcgttttta tgttgggtat caaatagctt gatgtaattg
    16321 ccataaagcg aaacagcaat aagcgaatgc tcgtgatgcc ttatttgtgt aattgaattt
    16381 gcacgcgaac cacccatgaa aaaactttgg tgcttgcttg ctgcataact gaccttagga
    16441 aaaactaatt caagtgttac ggtatccaat gtaaacaccg agcggttggt tcctatataa
    16501 attatgtttt tttcatcaag aaaaatgctg gtcaattcct gcctttcatc gcgataataa
    16561 aaatccttat gcagatagtt ttcatcattt gaatttctgt aaaaaaagcc acactgagtt
    16621 acaataaatt ctctcccgct tttcaaaata atcctgtcat ttgctttgca tgaagtagtt
    16681 ccgggcggaa aaggcagctt tgtaatttca aactgatttt gcagcgggtc gtaaacatac
    16741 aatcccgttt cggaggcaat ccatattcga ttgcgaatat cgcgatacag actatttatt
    16801 ctggatgtaa cgggatttga aaatttaata tcgttggtaa aggattttgt tttcaaatca
    16861 aaaaaacgaa tgcccgaaga ttttgtaccg caccataaaa ctttgttttc atcttctgca
    16921 aggcaaagta tttcatcgcc tgatagcttc aactttaaac ttctatcaaa aaaccaggtt
    16981 gccaatcttt ttccatttaa atcaaactga tgcaacccgt tatcccatgc accgcaccac
    17041 aaatgattat cgcttccaag aaatatttta ttaatagtca tagctgtgtc ggagacctgc
    17101 gttctgaatt tgccaatatt ccctaaccgt ttaagcgatt caataattcc tgcaaaatat
    17161 ggtatggtat cattaacaag tacatcataa taagtattgg tggaaatcag ctttttattt
    17221 ttatcagtag cttttgtttt taaatcgaat agctgcaagc cggcattatt ctgagcaatc
    17281 caaagacaat ttctgttttt atcaaatgtg atgtcgttga tgttatttac attcaattct
    17341 gaatttgact tattatacgt tttaaactta ccagttctga aatcataact acacatccct
    17401 gacgaagcgc ttccaatcca aagcaatgaa tcgttatcaa tttcaaggca agaaataata
    17461 ttaccgcaaa gtgaattgat gtccttttca ttgtgtttaa aaacatagaa gtcttgtcca
    17521 tcataacggt tcaatccatc cttagtacca atccatataa aaccctgttt gtcgtgaata
    17581 gtgcatgtaa cctggttgtc tgaaaggcct tgctgtgagg tgatacgtct taaagtaata
    17641 ttcttttgct gacttgaaga agcattggaa accaacatga aaagcaataa tgataaaatc
    17701 ttctttctca ttttattgtt agctttttgg ttgaattgtc acaagctgtg ttcaacagat
    17761 aagtgcaact cttaccttgt tttgtaaagg tacttcattt ggagataagt aactaagttt
    17821 tcttatatgt ctattatcga tttcgttttc aactctttcg acttcttttt ttttgattaa
    17881 agtgaagtct gttatttttg aaatgaatta tcaaaaaacg catttctatt tttcgatggt
    17941 accattatct tgcaatgtga atggtccggt aaaaataagt tatattttaa agtgcttctg
    18001 ctattttttc ttgatgtgta aatgcctggt cattgtctaa tatcatggtt tttctttgtg
    18061 tctggtattt gttttagttt gtgcgagaga tgtaacatag atttttgcac caaagcgtct
    18121 ttttggaaag ttgcgcttga gttatctaca tgcagtgctc ttgtgcacac tgagtgtaat
    18181 agaaatctgt gtttgtattt cgccagcaat tatataggct tcaattttgc a
//
LOCUS       DAORQA010000029        17871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_818_length_17871_cov_18.809609, whole genome shotgun sequence.
ACCESSION   DAORQA010000029 DAORQA010000000
VERSION     DAORQA010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17871
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_818_length_17871_cov_18.809609"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PK736_03700"
     CDS             complement(<1..550)
                     /locus_tag="PK736_03700"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRC32528.1"
                     /translation="MSFDLAPLLTTEGLISLLTLVLLEIVLGIDNIIFIAIICGKLPN
                     KQQKNARTIGLTLALAIRVLLLFTITWIVQATKPMFYISEFGVSGRDLILFAGGVFLI
                     VKTIIEIMHKFQAADDKTFKADKLTLTQAIIQITLIDVVFSFDSILTAVGLSREIAVM
                     VIAVIISMLVMLAFAPYVSDFIN"
     gene            complement(615..1637)
                     /locus_tag="PK736_03705"
     CDS             complement(615..1637)
                     /locus_tag="PK736_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRC32529.1"
                     /translation="MNNKQKKEEHQIVKKFTMVLLLLVGFAVLYSLRQFIDAFLGAVM
                     IYILFRPLMRHLVHKRKWNISLSAVLVILITILSVSIPSLLVIKVVRPRVVDFFNHPD
                     LLTNAFQYIDNKIFDLTGTKLINEDNLRNVQSSVAGYATTFLNETFSMITDLGIMFFI
                     LYFMLKSMGTMEQFFEDNLPLPSENIATFGEELRSMTMSNALGAPIMALIQGLIAVLG
                     YWICGLPDPFLWGIVTGVFSFVPIIGSALIWIPAALYILSLGATWQPVGIVIVGIIIS
                     VSDNILRFALQEKFADVHPIITVLGVITGFYYFGLPGLIFGPLLISWFIILFKIFRKE
                     YSFPQE"
     gene            1808..2107
                     /locus_tag="PK736_03710"
     CDS             1808..2107
                     /locus_tag="PK736_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRC32530.1"
                     /translation="MAVRYYYTYITTNPVKTTFYTGVTNNLVRRLSEHKANRGQFKTF
                     AGHYYCYNLVYYESYTSIKIAIEREKEIKDLSRDKKMELIKTKNPKMFFIDFKDE"
     gene            2453..2809
                     /locus_tag="PK736_03715"
     CDS             2453..2809
                     /locus_tag="PK736_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRC32531.1"
                     /translation="MKILGQILSSTLAIMAATYFLPHVHVDSPLYALLLAVVISALTI
                     LVKPVLIVLTLPATILTFGLFLFVINAVIVLMADYLVTGFHVEGFWWAMLFSIVVAAF
                     NSVLESLFFKDESRKS"
     gene            2919..3380
                     /locus_tag="PK736_03720"
     CDS             2919..3380
                     /locus_tag="PK736_03720"
                     /inference="COORDINATES: protein motif:HMM:NF037635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32532.1"
                     /translation="MKYKLTDAPSELPPLKVGDEVDLFITHESELGYSATINGDKEGL
                     IFRNEVFEPIVKGDYKRGYIKAIRDDGKIDLSLQKIGFKQVYDAKDVVLQEIKARKGK
                     LPLGDKSAPADIMRVMQMSKKVFKKALGILYKERLVVVGDFETLLTNNEPE"
     gene            complement(3384..3623)
                     /locus_tag="PK736_03725"
     CDS             complement(3384..3623)
                     /locus_tag="PK736_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013185932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HRC32533.1"
                     /translation="MLTNNDVLKKLRVALNLKDEDVIEILSLVDFKLTKSELSALYRK
                     EDHPNYRECGDQLLRNFLNGLIIYKRGPNPQQLKK"
     gene            3686..4447
                     /locus_tag="PK736_03730"
     CDS             3686..4447
                     /locus_tag="PK736_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRC32534.1"
                     /translation="MRDSITSPSAEKKNKFFTIAKTANYIFLAWLILCFKITDESKIL
                     SYVVNTNQQEIKLYYKNKNGQPYRSIQNLKNDVDSQNKQLIFAMNGGMYQQDNSPLGL
                     YIENKKLIAPLNTKSGSGNFYLKPNGIFYIDTKNKPHVCTTKNFKNKNVKYATQSGPM
                     LIIDGKINDAFKEGSQNLNIRNGVGIINDSTVVFAISKEEINFYDFAKFFQSIGCKNA
                     LYLDGFVSRAYVPEKKWLQTDGNFGVIIGITKAGK"
     gene            4613..7573
                     /locus_tag="PK736_03735"
     CDS             4613..7573
                     /locus_tag="PK736_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRC32535.1"
                     /translation="MCKYFFIFLIFLGVFGAFSLVPDRNVDPAFTSKESKQWVDSVYK
                     SLSTDQKIGQLFMVPAYAGGADYNKSAVDSLINNFYIGGLTYFLSGPERQVDLTNHFQ
                     AISKVPLLIAIDGEWGLNMRIDSTTRFPRQMTLGANPNEQLIYDMGVEIARQLKILGI
                     HMNYAPCVDINNNPMNPIINSRSFGENKEDVTRKASLYMKAMQDNSILACAKHFPGHG
                     NTETDSHSALPVINQSAEAMDTMELFPYKQLIKQGLGSVMIAHLNIPSLDSATNAPSS
                     LSYKIVTKILKDKLKFKGLIITDALNMKGVTANVPPGEIEVNALKAGNDMLLMAENVP
                     LAVSKIKEAFANKTLDSNDVFKSVKKILEIKYWCGLNDWKPLSKFMLNERLNNAPETR
                     KLTNSLFEKSVTILKNNNNTIPLSVDGHHKIASLVVNDTLDNYFQQSLDLYTHSENFA
                     LNKELSATAFDTLMNQLAQFETVIVSFHNTSIRSYINFNITPQMAQTITSLQGRTKLV
                     TVVFGNLYTLNILKDADKSQALILSYEDTYLPAMFTAQILFGARNAFGKLPARPSSSY
                     TLYEGLTGSTMSNVLRFEMPSRLSIDEKIFSRIDTIIKKNIDDKVFPGCQMLVAYKGN
                     IILNKGYGKFTYDNNSTAVNEQSLYDIASITKMAATSLAVMKLYDEGKIDLKQRMSFY
                     VPELRRSDKKNLLVEDILTHQSGLKGWIPFYQSTLDDKGNYKKGIYAATPVMPYSNKI
                     CDSLYMNVNYMKTIWKEILRSPLTERGKYIYSDLGMIITQKMVENITQQSLEEYVDEN
                     FYYPLGLNRIAYNPLQHFTINNIVPTENDTAFRKRLVRGYVHDPAAAMFGGVSGHAGV
                     FSNAHNLGIIMQMLLQGGAYSGRQYITTATVEKFTKQYWPNTNNRRGLFFDKPEANSK
                     EPVCSSASLNTFGHTGFTGTCAWADPNSELVFIFLSNRVYPSAANNRLVKYNTRSVLM
                     QVVYDALPKQSGSR"
     gene            complement(7623..8108)
                     /locus_tag="PK736_03740"
     CDS             complement(7623..8108)
                     /locus_tag="PK736_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32536.1"
                     /translation="MFYFLDKTSLTNNVLCPYNDTTSWGLYDGVKENTCKNMHVANLL
                     SELNYWTFGINNRQSADSIKIKSFQLIRNSGVIPILIINQEYTRIKANARNDSLIYLS
                     NGKYYDILPRSQSPFENKILFAAAPLKEKIYNGMTKFILSRDYFVSNLPAEIRKQTVQ
                     L"
     gene            complement(8194..8727)
                     /locus_tag="PK736_03745"
     CDS             complement(8194..8727)
                     /locus_tag="PK736_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32537.1"
                     /translation="MDATMYIKEYKTEKPIPYATVNIVRGLNSPQIIKTVTTDENGKA
                     IYCDDVDADYDYYFEAVADNYVDVFQIVPLRNGVRNYEATLYKYASSYVKLHIINVNP
                     FNQYDLLQFSTACSNFIQGANVDTVFLFCDNGNEFMGNFELYNYHCIANKNGIDSTIY
                     FSFIPPPHDTVTININY"
     gene            9280..10143
                     /locus_tag="PK736_03750"
     CDS             9280..10143
                     /locus_tag="PK736_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRC32538.1"
                     /translation="MRQLKITKSITNRESQSLEKYLQEIGREELITAEEEVRLARRIR
                     EGDQIALDRLTKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAKRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKISKAYSKLEQEFEREPSADELAK
                     LLDLPIEKITDTMKVSGKHVSMDAPFVQGEDNSLIDVLENADSPRADSLLMNESLQKE
                     IERSLGTLTEREREVIKLFFGIGLNHGLTLEEIGAKFDLTRERVRQIKEKAIRRLRHK
                     SRSRLLKAYLG"
     gene            10344..11819
                     /locus_tag="PK736_03755"
     CDS             10344..11819
                     /locus_tag="PK736_03755"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRC32539.1"
                     /translation="MDSTQASGANTAQAFEAQLQDWIKLEKAAVQLIAHMSHLWYDMS
                     VELLMFRNQMIDRSVSEIINLHHYAKTFVKKPITVYESVEVASEMAKMDLAPSRIDIG
                     KLTSEWVLEKANYASCKEFVADKLKNFIGKDKRVMVPKDVVLYGFGRIGRILARELIV
                     QAGKGEQLRLRAIVTRDKSDADIIKRADLLRSDSVHGPFPGTVIEDLPNKALIINGHV
                     VHMISAKQPEDIDYTIYGINDALVIDNTGILRDRVELSRHLVSKGVGKVLLTAPAAGD
                     VPNIVYGVNQSMCAENENIFSAASCTTNAIVPVLSVMDKMMGIDKGHIETIHSYTNDQ
                     NLLDNYHKKYRRGRSAPLNMVITETGADKAVSKAIPHLAGKITGNAVRVPTPNVSLAI
                     LNLTLNKVTNKDEINNALLNAALEGELVEQIQYSSNNELVSSDLVGCPASSIVDSPAT
                     IVSKDGKSAVLYVWYDNEYGYSRQCVRFSKYIAHVIRLTYY"
     gene            12047..12877
                     /locus_tag="PK736_03760"
     CDS             12047..12877
                     /locus_tag="PK736_03760"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32540.1"
                     /translation="MIKKLTSLCAIAFATISMQAQITGVQVGAVCPDFTGTSPNGTTH
                     NLYTYVSQGNYVLLDFFAYWCGPCKATAPKIEQFYKKYGCNTNGVIVLGNESDPVGTN
                     ALLAAFELQAGLDPINTYPAWSGQQGGGAAIGNVYSPSAYPTICLIGPDSTLINKDIW
                     PISTIADIEAAFPPGVLIPMPCVTSINEQQTFTLNSVFPNPTSDKLFITLADVTVNEL
                     TVEVFDILGNVTISKLVNPSASNNLIQLNISTLPQGAYMVRVSDGTSKVITSRFSVIK
                     "
     gene            complement(13387..15468)
                     /locus_tag="PK736_03765"
     CDS             complement(13387..15468)
                     /locus_tag="PK736_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32541.1"
                     /translation="MKNIAAIIFAYCVIIISHNTVCAQVVPENENDKQGTDKIELQAE
                     QDEDENVDYEELQLRLQKYSQNPLDLNRATTEEFKDFGLLTDLQINALQKHIDKNGNL
                     ISIEELQSVRYFDVEDIRGIQPYVNVGGSIDKVHIPFKTLITRSQKQLLLRSTYIVER
                     AAGYKSTEGEPAKYLGSPYGLYVRYRQSYSKKYSIGITAEKDAGEEFFKGSQKNGFDY
                     YSFHLVLNEIGKIKTLAIGDYQLSYGQGLTMWTGLAFGKSNDVSTLVRNPRGITPYTS
                     VNEMQFNRGVAVAYVLNKFQFDLFVSHRKIDANIPDQSTDSAQGEITFSSILLSGLHR
                     TNSELADKGAITQTNIGGHVSYKKRNLELGITAVNSQLDKPLKRTQYLYNQFQFEGDH
                     FFNVGFDYKYVAGNKLFFGEVAQSTNTGGWAMTHGTLISLDPRLSMSLMYRNFQRDYA
                     AISSNPVRESSVSNERGLLWGLTSKPVKGFSLAGYYDMFSFPWLRFRVDAPSSGYEYL
                     AQLNYTPSKKMDMFFRFRHQQKLQNSIDFSVKQDYLVPYDQKNYRFNFSFKATPALSI
                     RTRAEWTDVKFQNMTEHGFMLIQDVIVKPFGKPYSFNIRYVIFDTDGYNSRLYAYEND
                     VYGTFSIPPYYFKGSRYYINFRYKMHKGLDLFVRYGNTLYTNRTTIGSANDEIQGSTK
                     QDVRAALKFEF"
     gene            complement(15478..16359)
                     /locus_tag="PK736_03770"
     CDS             complement(15478..16359)
                     /locus_tag="PK736_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32542.1"
                     /translation="MKLLLHRFIFCFNGCNFYKKIISAFVVFLFFASTTISVYAGNGN
                     PHGGARDAGMAGASLTFGDAFSMFNNVAGMAWQQGFRAGLYNETRYGLKELSTNGLSV
                     SYGNTKSGSVGLGIKYFGYNQYNEQDISFGYARLLSTKISGGIQFHFLKTNFGDAYYG
                     DKSTLTVSLGMQYKVTEKLQFAFNIFNPTRNQLANYNNERFPTIVKAGFCWKPNEKVM
                     LCVEDEKSVQGKNIVKGGIEYHITKPLYLRTGIAGTPANGSFGLGLELKKFKIDIAAN
                     YDPVLGVTPHFSLLANL"
     gene            complement(16361..16867)
                     /gene="purE"
                     /locus_tag="PK736_03775"
     CDS             complement(16361..16867)
                     /gene="purE"
                     /locus_tag="PK736_03775"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002664527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRC32543.1"
                     /translation="MVGIIMGSQSDMPVMQAAADMLTKFKIPYEIQIVSAHRTPELMY
                     NYAFTARNRGLKVIIAGAGGAAHLPGMVASLTTLPVIGVPVKSSNSIDGWDSILSILQ
                     MPVGVPVATVALNAAANAGILATQILSINDENIAQTLRDYKTELKDKVTKSDPFKIAP
                     DKIPNKHS"
     gene            complement(16996..>17871)
                     /locus_tag="PK736_03780"
     CDS             complement(16996..>17871)
                     /locus_tag="PK736_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRC32544.1"
                     /translation="LQKRGKEVYPQPHIISMIQDKGLQKNFYNENNIPTAKYKLVNNK
                     TEIDWPFPYIQKMCTEGYDGKGVRKIDSAEELAQYGFTTPSVVEELVNFEKEIAVTVA
                     RNVSGDIVAYPPVEMKFNHEANLLDYLFAPSRLSPSQIKEASDIAIKIISQLEMVGIL
                     AVEMFVTSDGKILVNEMAPRAHNSGHQTIEANHTSQYEQILRAIYNMPLGSTEIITSV
                     VMFNLLGEKGFTGNAKYEGIDELLKVEGVHIHLYGKTITKPFRKMGHVTVTHNDIDEA
                     ISVGLTLKEKIKVKA"
BASE COUNT         5705 a         3342 c         3310 g         5514 t
ORIGIN      
        1 tatttataaa atcgctcaca taaggcgcaa atgccagcat cacaagcatc gaaataataa
       61 cggcaataac cataactgca atctcgcgcg aaaggccaac agctgttaaa atagaatcga
      121 aagaaaaaac tacatcaatg agtgtaattt gtataatcgc ttgtgttaat gtgagtttgt
      181 cggctttaaa agttttatca tctgcagctt gaaatttgtg cattatttcg ataatggttt
      241 ttacaataag aaaaacccca ccggcaaata aaattaaatc gcggccactt accccaaact
      301 cactaatgta aaacatgggt ttggttgcct gtacaatcca tgtaatagta aacaacaaca
      361 aaacgcgaat ggctaatgca agtgtaagac caatggtacg tgcatttttt tgttgcttgt
      421 tgggtagctt accacatatg attgcaataa aaataatgtt gtcgataccc aaaacaattt
      481 cgagcaatac cagggtaagt aaacttataa gaccttctgt tgtgagtaaa ggcgctaaat
      541 caaatgacat agaattttta ttttttacat taaaaagtcc gcaaagataa tatttgagag
      601 cagaaaaaga ttaatcattc ctggggaaat gaatactctt tgcgaaatat tttaaacaga
      661 ataataaacc atgaaataag aagcgggcca aagattaagc cgggtaagcc aaaataatag
      721 aaacctgtga taacacccaa tacagtgatg attggatgca catcggcaaa tttctcttgc
      781 aatgcaaagc ggagaatatt gtcgctcaca cttattatta taccaacaat tactattcca
      841 acaggctgcc aggtagcacc caacgaaaga atgtaaagcg ctgcgggaat ccaaattagt
      901 gcgctgccaa tgatgggtac aaacgaaaat actcctgtaa caattcccca caaaaaggga
      961 tcgggcaggc cgcaaatcca ataacccaga acagctatga gcccttgtat aagtgccata
     1021 ataggtgcgc caagtgcgtt agacatggtc atgctacgca gttcttcgcc aaaggtggca
     1081 atattttcgg aaggtaaggg aagattgtct tcaaaaaatt gttccatggt tcccatcgat
     1141 ttcagcataa aatataaaat gaaaaacata ataccaaggt cggtaatcat gctaaaagtt
     1201 tcgtttagga aagtagtggc ataaccggcc acagatgatt gcacattgcg aagattgtct
     1261 tcgttaatta attttgttcc tgtaaggtca aaaattttat tgtcgatgta ttgaaatgcg
     1321 ttggtcaaca aatcgggatg gttaaaaaaa tcgacaacac gaggccttac tacttttata
     1381 accaaaagac ttggaatgct tactgataga atggtaatga gaataaccaa aacggcactg
     1441 agcgaaatgt tccacttacg cttatgtaca agatgacgca tcaacgggcg aaacaaaata
     1501 taaatcatca ctgcgccaag aaaagcatcg atgaattgcc ttaacgaata caaaaccgca
     1561 aaaccaacca atagcaaaag taccatggtg aattttttca ctatttgatg ctcctctttt
     1621 ttttgtttat tgttcatata tttttctttg atgattgatg ctgaaatcgt aaaaatgttt
     1681 cacacaaaag tagtttttta aatattgttt aagcaccatt ttgttaaact gtttttgaat
     1741 ttatgcgtaa taattttctt ctttataagg ataatagaaa ctattttcgt gcataagaat
     1801 taacaatatg gcagttcgct attattatac atatatcaca acaaaccccg ttaaaacaac
     1861 tttctatact ggtgttacaa ataatttggt cagaaggctt tcggagcaca aagcgaacag
     1921 aggacagttt aaaacatttg ccggccatta ttattgctat aatttggttt attatgaaag
     1981 ttacacatca ataaaaattg ctatcgagcg agaaaaagaa ataaaagatt tatcgcgtga
     2041 taaaaaaatg gaattgataa aaacaaaaaa tccaaaaatg ttttttattg actttaaaga
     2101 tgagtgaaac ctgacacctc aggtcattca gtaggaacct tactaataca ttagataaat
     2161 taattcaaaa caaattcctc taaatataca agaagattcc tcctgaaaga ctggcggggc
     2221 gtaacgtcat tcaggaagaa acttatttct acgttagata aaattgtttt caaaacaaaa
     2281 tctcctaaac ttacaagaag gttcctcctg aaagactggc gtggcgtagc gtccttcaac
     2341 atctattttt ttctaaagtg cattacttta tattcaatct attttgcttt actttgtaag
     2401 taaataataa ttcaatattt aatttttcaa taaaagcata caatttattt ttatgaaaat
     2461 tttaggacag atactttctt ctactttagc aataatggcg gctacttatt ttttgcccca
     2521 tgtgcatgtt gattctcctt tgtatgcatt gctgcttgca gttgttattt ctgctcttac
     2581 aatactggta aagcctgtgt tgatagttct tactttgcct gctaccattc ttacattcgg
     2641 attgtttctg tttgtaataa atgctgtgat tgttttaatg gccgattatc ttgttacagg
     2701 ttttcatgtt gaaggatttt ggtgggccat gctttttagt attgttgtgg ctgcattcaa
     2761 ttcggtttta gaatcgttgt tctttaaaga tgaatcgcga aaatcctgaa gataaattcc
     2821 ggcaagaaaa aagaataata aaatatcaat tgatgattta tcgaattatg aaagcagtga
     2881 cacagtaatt cgaattgaat agtgtaacga aaaaaaatat gaaatataaa ttgaccgatg
     2941 ccccttccga attgccaccg ctgaaagtag gtgacgaagt agatttattt attacccacg
     3001 aatcggaact aggttatagt gctaccatta atggcgataa agagggactg atatttcgca
     3061 acgaagtgtt tgaacccata gtgaaaggag attacaagcg tggctatata aaggccattc
     3121 gcgatgatgg aaagatagat ttgagtttgc aaaaaattgg attcaaacaa gtgtatgatg
     3181 ccaaagatgt tgtgttgcaa gaaataaaag cgcgtaaagg aaaattgcca ttgggcgata
     3241 aaagtgctcc ggccgatatt atgcgtgtga tgcaaatgag taaaaaagta tttaaaaaag
     3301 cattgggcat tttgtataaa gaaagactgg ttgttgtagg ggatttcgaa acgctgctca
     3361 cgaataatga gccggagtaa aaattatttt ttcaattgtt gcggatttgg tccacgttta
     3421 taaataatta atccattcaa aaaattgcgc aaaagttgat cgccacattc gcggtagtta
     3481 ggatgatctt ctttacggta caaggcactg agttcgcttt tggtaagttt aaaatcgaca
     3541 aggctaagta tttcaatcac atcctcgtct ttaaggttaa gggccacacg caattttttc
     3601 agcacatcgt tattggtaag catactttgt ttttttttga ttgatgtaaa gatgaaaaaa
     3661 tacatgttat aattgcaaat aaaaaatgag agattctatc acaagcccat cagctgaaaa
     3721 aaagaataaa ttttttacca tcgcaaagac cgctaactat atatttttag catggctcat
     3781 tttgtgtttc aaaattacgg acgaaagcaa aatattgagc tatgttgtaa ataccaatca
     3841 gcaggaaata aaattgtatt acaaaaataa aaacgggcaa ccatatcgct caatacagaa
     3901 tttaaaaaat gatgttgata gccaaaacaa acaattgatt tttgccatga atggcggtat
     3961 gtatcaacag gataattcgc cactgggttt atatattgaa aacaaaaaac tgatagcgcc
     4021 attaaataca aaatcgggca gtggcaattt ctatctcaaa cctaatggca ttttttatat
     4081 tgatactaaa aacaaaccac acgtttgcac taccaaaaat tttaaaaata aaaatgtgaa
     4141 gtacgcaaca caaagtggac ctatgttaat cattgatggt aaaataaacg atgcatttaa
     4201 agaagggtcg caaaacttaa atattcgcaa tggagtagga ataataaatg acagcactgt
     4261 agtttttgcc atatcgaaag aggaaattaa cttttatgat tttgcaaagt tctttcaaag
     4321 cattggttgt aaaaatgcat tgtacctcga tggatttgtt tcgcgggcat atgttcccga
     4381 aaaaaaatgg ttgcaaactg atggcaactt cggagtaata attggtataa caaaagccgg
     4441 gaaataaaat ttttgctttg ctttcggccg tatttatttt cgtgtagctt tattattttt
     4501 caaatccatt tcctatttag ttgaatgatt cacgcgtact tgtttattta tttagttgta
     4561 aagtccaaaa aaataatttg gtttcgtaca tttggccctt gattataatt caatgtgcaa
     4621 atactttttt atttttttaa tttttcttgg cgtgtttggc gcatttagtc ttgtacctga
     4681 tcgcaatgtt gatccggcat ttacaagcaa agagtccaag caatgggtcg attctgttta
     4741 caaatcgctc agcaccgacc aaaagattgg ccagttgttt atggtgcctg cttatgccgg
     4801 tggtgccgat tataacaagt ctgcagtgga ctctctcatc aataattttt acataggtgg
     4861 gctaacgtat tttttgagcg ggcccgagcg gcaagttgat ttgacaaatc attttcaagc
     4921 aataagtaaa gtgccattgc ttattgccat cgatggtgaa tggggattaa atatgcgtat
     4981 agacagcacc acccgttttc cacggcaaat gactttgggt gcaaacccca atgagcagtt
     5041 gatttatgat atgggtgttg aaattgcacg gcaattgaaa attttgggta ttcacatgaa
     5101 ctacgcacct tgcgttgata tcaataacaa cccgatgaat cccatcatca actcacgttc
     5161 gtttggcgaa aacaaagaag atgtaacccg caaagcttca ttgtatatga aggctatgca
     5221 agacaatagt atacttgcat gcgcaaaaca tttccctggt catggcaaca ccgaaaccga
     5281 ttcgcacagt gccttgccgg tgattaatca atctgccgaa gctatggaca cgatggaact
     5341 ttttccttac aaacaattga taaagcaagg tttggggagt gtgatgattg cacatctgaa
     5401 tattccttca ctcgattcag caacaaacgc accttcgagt ttatcatata aaatagtaac
     5461 gaaaatttta aaagataaat taaaattcaa aggacttatt attaccgatg cgttgaatat
     5521 gaaaggtgta acagccaatg tacctccggg cgaaattgaa gtaaatgcat tgaaagcagg
     5581 aaacgatatg ctgctaatgg ccgaaaatgt gccgcttgct gtaagcaaaa taaaagaagc
     5641 atttgcaaac aaaacactcg atagtaatga tgtatttaaa agcgtaaaaa aaattcttga
     5701 aataaaatat tggtgtggat tgaatgattg gaaaccactt tcaaaattca tgcttaatga
     5761 acgcttgaac aatgcacccg aaacgcgtaa gcttacaaat tcgttgttcg aaaaatcggt
     5821 tacgatttta aaaaacaaca acaataccat tccactttct gttgatgggc atcataaaat
     5881 tgcatcgctt gttgtaaacg atacacttga taattatttt caacagtcgc tcgatttgta
     5941 tacacactcc gaaaactttg cgctcaataa agaattgtct gcaaccgctt tcgatacgct
     6001 catgaatcag cttgcgcaat tcgaaacggt tatagttagt tttcataata cttctataag
     6061 gtcgtatatc aatttcaaca ttacaccgca aatggcacaa accattactt cattgcaggg
     6121 gcgcacaaaa cttgtaacgg ttgtgtttgg taatctatac accctcaata ttctcaaaga
     6181 tgccgataaa agtcaagccc ttattttgtc atacgaagat acttacttgc cagccatgtt
     6241 cacggcacaa atattatttg gcgcacgcaa tgcctttgga aagttgcccg caaggcctag
     6301 cagtagttat acattgtatg agggcttaac gggctctacc atgtcgaatg tattgcggtt
     6361 tgaaatgcct tcgaggctct ctatcgatga aaaaatattt tcgcgcatag ataccatcat
     6421 caaaaaaaat attgacgaca aagtttttcc cggatgtcaa atgttggttg cgtataaagg
     6481 caatattatt ttaaataagg gatacggaaa gtttacttac gataataatt ctactgctgt
     6541 aaatgagcaa tcactttatg atatagcatc aattacaaaa atggctgcca cttcgcttgc
     6601 ggtaatgaaa ttgtatgacg aaggaaaaat agatttgaaa cagcgcatgt ctttttacgt
     6661 tccagagttg cgaagaagcg ataaaaaaaa tttattggtc gaagatattc ttacacatca
     6721 aagcggactt aaaggatgga taccttttta tcaatcgaca cttgacgaca aaggcaatta
     6781 taaaaaaggt atttatgctg ccacacctgt aatgccatac agtaataaaa tttgcgatag
     6841 tttatacatg aatgtaaact atatgaaaac tatatggaaa gaaatattgc gatcgccact
     6901 taccgagcgt ggcaaatata tttactctga tttaggaatg attattacac aaaaaatggt
     6961 ggaaaacatc acacaacaat cgctcgaaga atatgttgac gaaaattttt attacccgct
     7021 tggccttaat cgaattgctt ataatccttt gcaacatttt acaataaaca atatagtgcc
     7081 aaccgaaaac gatactgcgt ttcgcaaacg attggtgcgt ggttatgtgc acgaccctgc
     7141 tgcggctatg tttggcggtg taagtggcca tgccggtgtt ttttcaaatg cgcataacct
     7201 tggtatcatt atgcaaatgt tgctgcaggg aggggcttac agtggcaggc aatatattac
     7261 aaccgctaca gtcgaaaaat ttaccaaaca atattggccc aataccaaca accgcagagg
     7321 attgtttttc gataagcccg aagcaaattc caaagaacct gtatgcagca gcgcatcgct
     7381 caataccttt ggccatacag gttttacagg cacctgtgca tgggccgatc caaacagcga
     7441 actggttttt atttttctca gcaaccgcgt atatccatca gctgctaata acaggttggt
     7501 aaaatacaat acacgatcgg tgctaatgca ggttgtgtac gatgcgttgc ccaagcaaag
     7561 tggcagcaga taaaacttta gtcattgcta gtcgagaatc gataattatt gttgggttgg
     7621 ttttataatt gaaccgtttg cttacgaatt tcagcgggta aatttgaaac aaagtaatct
     7681 ctacttaaaa taaatttagt catcccattg tatatttttt ctttaagtgg tgcagcagca
     7741 aaaagtattt tattttcgaa tggactttgg cttcgtggta aaatatcata atactttcca
     7801 ttgctcaaat aaattaaact atcatttctt gcattagctt ttatccttgt atattcttga
     7861 ttgataatca gtataggaat aacaccactg ttacgaatta attgaaacga ttttattttt
     7921 atactgtcgg ccgattgtcg attattgatg ccaaaagtcc aataatttaa ttcgcttaat
     7981 aagtttgcaa catgcatgtt tttgcatgtg ttttctttca caccatcata caatccccac
     8041 gatgtagtgt cattgtaagg acacaagaca ttgttggtta aagatgtttt atcgagaaaa
     8101 taaaacatac tgtcagtttg tgcgataata ggccatagtt tgcccagcca tacaaaatgg
     8161 cgataagaat tctctttttc ataattttat tttttagtag tttatgttta tagtgactgt
     8221 gtcatgcgga ggtggtataa aagaaaaata aatcgttgag tctatgccat ttttatttgc
     8281 tatacaatga taattatata attcaaaatt acccatgaat tcatttccat tatcgcaaaa
     8341 caaaaaaaca gtatcaacat tcgctccttg tataaaattg ctacaggctg tcgagaattg
     8401 caataaatca tattgattaa aaggattcac atttataata tgtaacttca cataactact
     8461 agcatacttg tataaagtag cctcgtaatt tcgcactccg tttcgcaacg gaactatttg
     8521 aaaaacgtca acataattat cggccacagc ttcaaaataa taatcgtaat ctgcatccac
     8581 atcatcgcaa taaatagctt tgccattttc gtctgttgtt actgttttaa taatttgggg
     8641 gctatttaat cctcttacta tgttaactgt ggcatagggt attggttttt cggttttata
     8701 ttcttttata tacatagtgg catccatttt attgcgactt atttttgagc aactgctggt
     8761 cagaaaaaca atgcaaaaaa taaaaatcaa aaacctattt tgcttactca taaaatttat
     8821 gaattaattg gtttacaaaa ctataataaa atttcagata tgaaatttcc aaaatggaaa
     8881 taataatgaa agtttggcaa taaattacaa gggtttaaaa tcattactct aagtaacatc
     8941 acctgctcta aagtctaaat cttatgttat ttttcaacag ccataagtaa atatgacctt
     9001 tagttttaca atcttataac ctgtttgcta tcgggtatat aaaaatttct tgtcaatgca
     9061 ttttaatctg tttagttcaa caaatttttt tcatcaagct tttgctttaa ccattttaaa
     9121 aaaaatcacg cctttaacat cacattttac aaaacaagta cagttattgc aatagggtgt
     9181 ttgcagcaaa aaaaatgaaa tttgacatta tgtcgtaaaa cattactttt gcgcgcttgt
     9241 ttatataaaa gagattaaaa aaagagggaa aataaaacaa tgaggcaact aaagataacc
     9301 aaatccatta ccaatcgcga gagtcaatcg ctggagaaat atctacaaga aataggacgt
     9361 gaagaactta ttacagccga agaagaagtt cgcctggcgc gaagaatacg cgaaggcgac
     9421 caaatagccc tcgaccgcct caccaaagca aatttgcgtt ttgtggtatc ggtagctaag
     9481 caataccaaa atcagggatt gagtttgccc gacttaataa acgaaggaaa tctgggatta
     9541 ataaaagctg ccaaacgttt tgacgagacc cgtggtttca aatttatatc gtacgctgta
     9601 tggtggattc gtcagtcaat attgcaggca ttggcagaac agtcgcgtat tgtgcgtttg
     9661 ccattgaatc aggtgggctc gttaaataaa atatccaaag cttattcgaa acttgagcaa
     9721 gagtttgagc gcgaaccttc ggccgatgaa cttgccaaat tgctcgactt gccaatcgaa
     9781 aaaataactg ataccatgaa agtatcgggc aagcatgtat ctatggatgc accatttgtg
     9841 caaggtgaag acaatagtct tattgatgta cttgaaaatg ccgattcgcc acgtgccgat
     9901 tctttattga tgaacgaatc gttgcaaaaa gaaatagagc gttcacttgg tacacttacc
     9961 gaaagagagc gtgaggtgat aaaattattt ttcggcatag gcttgaatca tggtttaaca
    10021 ttggaagaaa taggagcaaa gttcgaccta acccgcgaac gcgttcgtca gataaaagaa
    10081 aaagccatac gcaggttgcg acacaaatcg cgcagccgtt tgctgaaagc atatttagga
    10141 taaaatttaa agcccgcata gtcgggcttt ttttatgctt tgtgtatttt caacactatg
    10201 catttcatcg gataaaatgc cttccttttg atagcagcaa ggtgaaataa tttatgagaa
    10261 aatattttga agaaaaaaat tacaaatcga aagaagtttc acatttttga catcgataaa
    10321 aaacatacct taataaaaat aatatggata gtacacaagc aagcggagca aataccgcac
    10381 aagcgtttga ggcacagctt caggattgga taaaattgga aaaagctgcc gtacaactta
    10441 tagctcacat gagccatttg tggtacgata tgagtgtgga actgctgatg tttcgtaacc
    10501 aaatgattga ccgcagcgtg agcgaaatta ttaacctgca tcactatgca aaaacctttg
    10561 taaaaaaacc tattaccgtt tacgaatcgg tagaggtggc aagcgaaatg gcaaaaatgg
    10621 atcttgcacc atcgcgtatt gatataggta aacttaccag cgaatgggtt ttggagaaag
    10681 ccaactatgc ttcttgtaaa gaatttgtgg ccgataaact caaaaacttt attggcaaag
    10741 acaagcgtgt aatggtgcct aaagatgtgg tgctatatgg ctttggtcgc ataggacgga
    10801 tacttgcccg tgagttgata gtgcaggccg gcaaaggcga acaacttcgt ttgcgcgcaa
    10861 ttgttacccg cgataaaagc gatgccgata ttattaagcg tgccgatttg ttgcgctccg
    10921 attcggtgca tggccctttt cccggaactg taatagaaga cttgcctaac aaagcactta
    10981 ttataaatgg ccacgtggtg catatgataa gcgccaaaca acccgaagat attgactata
    11041 ctatttatgg cattaacgat gcactggtga tagacaatac cggtatactg cgtgacagag
    11101 tagagcttag ccgccatctt gtttcgaaag gtgtgggtaa agtgttgctt actgcacctg
    11161 ccgctggcga tgtgccaaat attgtttacg gtgtcaatca atcgatgtgt gccgaaaatg
    11221 aaaatatttt ttctgctgct tcgtgtacta ccaatgctat tgtgcctgtg ctttcggtta
    11281 tggataaaat gatgggcata gacaagggcc atattgaaac cattcactca tacacaaacg
    11341 atcaaaacct gctcgataac tatcacaaaa aatatcgcag aggacgcagt gcacctctaa
    11401 atatggtaat taccgaaacc ggtgccgaca aagctgtttc gaaagctata ccacatcttg
    11461 caggaaaaat taccggtaat gccgtgcgag taccaacacc aaatgtatcg ttggcaattt
    11521 taaatctcac cttaaacaaa gtgaccaaca aggatgaaat aaataatgcc ttattgaatg
    11581 ctgcattgga gggtgaattg gtcgaacaaa tacaatactc aagcaacaac gaacttgtaa
    11641 gcagcgacct tgtgggatgc cccgcatcga gcattgttga tagccctgca acaatagtat
    11701 cgaaagatgg taagagtgca gtgttatatg tttggtacga taacgaatat ggttacagcc
    11761 gccaatgtgt gcgtttttca aagtacatag cacatgtaat tcgccttact tactattaga
    11821 aaatttgaac tgaaatagca aattattaaa ataatgctgc gttatttatt taacgcagca
    11881 ttttttttgc aatcattcgc aagcgttttt aaatttcttt ccctcactag ccaactaatc
    11941 acaaatttta ctttctgttt tttcttgcaa aaagaatggg tcaaaagttt atgtcttaat
    12001 tttaattcct acttttacac atcaaaacaa ttttaaaaaa taaacgatga ttaaaaaact
    12061 tacttcatta tgtgctatcg catttgcaac aattagcatg caagcacaaa tcaccggtgt
    12121 gcaggttggc gctgtatgcc ccgattttac aggcacatcg ccaaacggca caacgcacaa
    12181 tctttacacc tatgtgtcgc agggcaatta tgtgttactc gatttttttg cttactggtg
    12241 tggcccatgt aaagccacag ctccgaaaat tgagcagttt tataaaaaat acggatgcaa
    12301 taccaacggt gtaattgtgc ttggcaacga aagcgatcct gtaggtacca atgcattgtt
    12361 agcagccttc gaattgcagg ctggcctcga tcctattaat acttatcctg catggagtgg
    12421 ccaacaaggt ggcggtgctg ctataggcaa tgtatatagc ccttctgcat atcctactat
    12481 ttgtcttatc ggccccgact ctacattgat aaacaaagac atttggccaa tcagtaccat
    12541 tgcagatata gaagcggcat ttcctcctgg tgtgcttatt cctatgccat gtgttacaag
    12601 cattaacgag caacaaacat ttacactcaa cagtgtgttt ccaaatccta cttctgataa
    12661 actttttatc accctggctg atgttacagt gaacgaactt acagttgaag tttttgatat
    12721 tttaggaaat gtaacgattt caaaacttgt aaatccatct gcaagtaaca atcttatcca
    12781 attgaatatt tctactttgc cacaaggcgc ttacatggtg cgcgtaagtg atggaacttc
    12841 aaaagtaatt acatcgcgct tctctgttat caaataaata tttctattct atcaatatta
    12901 aaagccggag tgtttgctcc ggctttttta gttataatta ttttgggttt attctttttc
    12961 aaacaatttt acaaccttat cacaatctcc gctttctaaa aaattattct ttaataaact
    13021 ttttactcaa ccgctcttac tcaatactca aatgcacaat atgcatccca cttgcccaac
    13081 ctgcacaatc cacatcaact gtaaagtatc ccgaattaac tcttgaatgc cgacacttcg
    13141 gtcggcatcc cgaccgtagt gtcgggacac ttaaactctt gaacaattaa actatttatt
    13201 ccgttgacta aagccgaacg gcaatgaatg tttcgcgaaa gcattgataa cttttaaact
    13261 ccttaaccct tgaactttta aactattaat tccgttggct aaagccgaac ggcaatgaat
    13321 gtttcgcgaa agcattgcaa ctattaaact cttgaactct taaacttcct gccccccccc
    13381 ccaacttcag aactcaaact taagcgcagc ccttacatct tgttttgtgc tgccttgtat
    13441 ttcatcgttg gcacttccta ttgtggtgcg gttggtgtac agcgtattgc catagcgcac
    13501 aaaaaggtcg aggcctttgt gcattttgta acggaagttt atataatatc ggctgccttt
    13561 gaaataatat ggcggaatgc taaaggtgcc atacacatca ttttcgtaag catacaatcg
    13621 cgagttgtat ccatcggtat cgaatattac atagcgaata ttgaaagagt aaggcttgcc
    13681 aaaaggtttt acaatcacat cctgaatgag cataaaacca tgctcggtca tgttttgaaa
    13741 ttttacatcg gtccactcgg cacgtgtgcg aatgcttagt gccggtgtgg ctttgaaaga
    13801 aaagttgaag cggtaatttt tttgatcata aggcacaagg taatcttgct ttacactgaa
    13861 gtcgatactg ttttgtaatt tttgctgatg acgaaaacgg aaaaacatat ccatcttttt
    13921 tgatggagtg taattcaatt gtgccaaata ttcgtaacca cttgatggtg catctactct
    13981 aaaacgcaac catggaaagc taaacatatc atagtaaccc gccagtgaaa atccttttac
    14041 aggtttcgaa gttaagcccc acaacaaacc acgttcgttg ctcacgctac tttcgcgcac
    14101 agggttgctc gatatagctg catagtcgcg ttgaaaatta cgatacataa gcgacatgct
    14161 caagcgtggg tccaggctta tgagtgttcc atgtgtcata gcccaaccac cggtatttgt
    14221 tgattgcgcc acttcgccaa aaaataattt attgccggcc acatatttat aatcgaaccc
    14281 aacattaaaa aaatgatcgc cctcgaattg aaattgatta tacaaatact gtgtacgttt
    14341 taatggctta tcgagctgac tatttactgc ggtgatgcct aattccaaat tgcgtttttt
    14401 ataacttacg tgcccgccaa tattagtttg tgtgatggca cctttatcag caagctcgct
    14461 attggttcga tgcaagcctg ataataaaat agaggaaaaa gtaatttctc cctgtgcaga
    14521 gtcagtactt tggtcaggaa tatttgcgtc tattttgcga tgcgacacaa acaaatcgaa
    14581 ttgaaattta tttaatacat atgccactgc cacaccacga ttaaattgca tttcgtttac
    14641 cgatgtatat ggagtaatac cacgcggatt gcgcacaagc gtactcacat cattcgattt
    14701 gccaaacgct aagccggtcc acattgtaag tccctgtccg taagataatt gataatcgcc
    14761 aatggcaagg gtttttattt ttcctatttc gttcagtact aaatgaaatg aataataatc
    14821 gaaaccgttt ttttgtgagc ctttaaaaaa ttcttcaccg gcatcttttt cggcagtgat
    14881 acctatgctg tatttttttg aatacgattg gcgataccga acatacaatc catatgggct
    14941 gccaagatac tttgccggtt caccttctgt tgatttataa cctgccgcgc gctccactat
    15001 ataagttgag cgcaacaata attgtttttg gcttcgggta attaatgttt taaatggaat
    15061 atgcactttg tcgatactgc caccaacatt tacataaggt tgtataccac gtatatcctc
    15121 aacatcaaaa taacgcaccg attgtaattc ttctatcgag attaaatttc catttttgtc
    15181 gatatgtttt tgcaacgcat ttatttgcaa atcggtaagc agcccaaaat ctttaaactc
    15241 ttcggtggtg gcacgattga ggtcgagggg gttttgtgaa tatttttgta agcgcaattg
    15301 aagttcttcg taatctacat tttcgtcttc gtcctgttcg gcttgcaatt ctattttgtc
    15361 ggtaccttgt ttatcgtttt cgttttcggg taccacttgt gcgcacacag tattatgtga
    15421 aataattatc acacaataag caaaaataat ggcagcaata tttttcattt atgcatatta
    15481 taaatttgcc aacagagaaa aatgtggagt aactcccaat accggatcat agttggcagc
    15541 aatatcaatt ttaaactttt ttaattcgag ccccaatcca aacgagccat tggcaggcgt
    15601 tcccgctatg cctgtgcgca aatagagtgg ttttgtaata tggtattcta tgcccccttt
    15661 aacaatattt tttccctgta cacttttttc atcctctaca catagcataa ctttttcgtt
    15721 gggcttccag caaaaacctg ccttaacaat ggtaggaaaa cgctcgttat tataattggc
    15781 taattggtta cgggtggggt taaaaatatt aaaagcaaat tgcaactttt cagtaacctt
    15841 atattgcata ccaagcgaaa ctgttaatgt agatttgtca ccataatagg catcgccaaa
    15901 attggttttc aaaaaatgaa attgaatgcc accacttatt tttgtactaa gcaagcgtgc
    15961 ataaccaaac gaaatgtcct gctcgttgta ttgattgtaa ccaaaatatt ttatgcccaa
    16021 tcctacactt cccgattttg tattgccata tgaaactgac aagccattgg tcgataattc
    16081 tttcaagcca tagcgcgttt cattatacag cccggctcta aagccttgct gccatgccat
    16141 gcccgccaca ttattgaaca tggaaaatgc atcgccaaat gttaacgatg cacccgccat
    16201 accggcatca cgcgcaccgc catgcgggtt gccattgcct gcgtatacac ttatagttgt
    16261 tgaggcaaaa aataaaaaaa caacaaaagc gctgattatt tttttgtaaa aattacaacc
    16321 attgaaacaa aaaataaatc gatgtaatag aagtttcata ttaagaatgt ttgtttggaa
    16381 ttttatcggg cgcaatttta aaagggtcgc ttttagtgac tttatctttc aattcggttt
    16441 tgtaatcgcg caaagtttgg gctatgtttt catcgttgat agataatatt tgtgtggcta
    16501 atattcctgc attggcagcg gcattcaatg ctacagtagc aacaggcaca cccacaggca
    16561 tttgcaaaat cgataaaata gaatcccaac catcaatgga gtttgacgat tttaccggta
    16621 cgccaattac aggaagtgta gtaagtgaag ccaccatgcc gggtaaatgt gccgcacctc
    16681 ccgcaccggc aattattact ttgaggccgc ggttgcgtgc agtaaatgca tagttgtaca
    16741 tcaactcagg cgtgcggtgt gccgatacta tttgtatttc gtatgggatt ttaaattttg
    16801 taagcatatc ggcagcggcc tgcatcacag gcatatcgct ttgactgccc ataattatac
    16861 ctaccaatgc atccgatata ttttcacttt tcttcattcg gtatagtctt caatattgtt
    16921 tgactttttt caagtagaat ttctacacgt accaaacata aacaaaatat tattacttca
    16981 ataaattata ttttgtcagg cctttacttt tattttttct tttaaggtta atccaacaga
    17041 aatagcctca tcaatatcat tatgtgtaac ggtaacatgt cccatctttc tgaagggttt
    17101 tgtaatagtt ttgccataga gatgaatgtg tactccctcc acttttagta attcatctat
    17161 gccttcgtat ttggcattgc ctgtaaatcc tttttcacca agcaggttaa acatcaccac
    17221 actggtaata atttcggtgc tgcccaaggg catattgtaa atagcacgca gtatttgttc
    17281 gtattgtgat gtgtgattgg cctctatagt ttgatgcccg ctgttgtgtg cacgtggtgc
    17341 catttcgttt acaagtattt ttccatccga agttacaaac atttctacag cgagtatgcc
    17401 caccatttcg agttgcgaaa taatttttat agcaatgtca cttgcttctt ttatttgtga
    17461 tggcgataaa cgtgaagggg caaataaata atcgagcagg tttgcttcgt ggttaaattt
    17521 catttctacc ggtggatagg caacaatatc gccacttaca tttcgcgcta ctgttacagc
    17581 aatttctttt tcgaagttta caagttcttc caccacgctt ggcgtagtaa atccatattg
    17641 ggcaagctct tcggcactat caatttttct cacaccttta ccatcatatc cttcggtgca
    17701 cattttttgt atgtatggaa acggccagtc tatttctgtt ttattattta ctaatttata
    17761 ttttgctgtt gggatattat tttcgttgta gaaatttttc tgcaatcctt tatcctgaat
    17821 catcgaaatt atatggggct gtggatacac ttccttacca cgcttttgca a
//
LOCUS       DAORQA010000030        17656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_831_length_17656_cov_24.427589, whole genome shotgun sequence.
ACCESSION   DAORQA010000030 DAORQA010000000
VERSION     DAORQA010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17656
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_831_length_17656_cov_24.427589"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..446)
                     /locus_tag="PK736_03785"
     CDS             complement(141..446)
                     /locus_tag="PK736_03785"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRC32545.1"
                     /translation="MVQIKWLQSAKNDLREIYDYISKDSKRYAQLQVEKIRTRTEILK
                     NQIEIGKVVVELENENIRELIEGNYRIIYRITSKHEIHILMIQHGARDLSRRIEFGV"
     gene            complement(434..616)
                     /locus_tag="PK736_03790"
     CDS             complement(434..616)
                     /locus_tag="PK736_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32546.1"
                     /translation="MITKEKLKIHIESFPENISIDELIERLIFIEKLENRIAESQQDK
                     TISEEELKLEMAKWFK"
     gene            803..2875
                     /locus_tag="PK736_03795"
     CDS             803..2875
                     /locus_tag="PK736_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRC32547.1"
                     /translation="MKKQHKILFLFLIQNILAITIYAQGGTWTWISGSSNANALGVFG
                     TQGIPSVNNHPPAVYEPCEWKDKQGNFWIYGGTYNSYCDLWKFNPTTLEWTWVKGSGL
                     QNMPTYYGTKGVANISNSPGVRSFCSITWVDTSGNFWIFGGANSSNALWKYDIANNMW
                     TWVNGTTNTNAYGVYGIKGVPAASNVPGIRKETCSGWTDSLNNLWLFGGFGNGVSALG
                     YLDDLWKYNIATNEWTWMAGDFGINKLANYGVKGVSSATNNPGGRMSYTKWKDAGGSF
                     WIFGGSDSKVKNDVWKYQPDINQWVWMNGKSTNNDSGYYNSFCAFDSTLLPFSRFEHR
                     SSVTDNCGNFWMYGGFRSGAFPQNDLWFFDPQILQWKLLNGTNKFFQPGNYGLLGVPS
                     VNNAPPSRGGASAWWCDDNKFYMFGGLYSIVKSYADVWVFDPDTNCISFNCNNSTSAI
                     ANFSSSDSLFCEKQCIDFTDISQNNPTSWQWTFNGASPSTSTDQNPTSICYNSYGSFD
                     VTLVACNQFGCDTITKNNFITSYITPFDSIWFANDTLFALPAYSYQWYAVGAGLIANA
                     TQNYYVPLTLGSYYCTTKDSDGCEATSNVILYTSFFENENNELANAVWPNPANEILFV
                     NPQLLNAKNIIFEIYDMQGRIILQQMLSNNINSINISQLATSVYSYRIMTIEKIIAQG
                     KFVKQIKN"
     gene            3745..5868
                     /locus_tag="PK736_03800"
     CDS             3745..5868
                     /locus_tag="PK736_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005872254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HRC32548.1"
                     /translation="MIQALVKKGKVIGEVVPAPVVSDGCLLIQVVNSCISAGTELTSV
                     QTSKKSLIKRAMEQPEEVKKVLDFVRSNGIEKAYLRVKGVMDTGKPTGYSIAGRVIAV
                     GKGVTGYEAGDCVAAAGAGIANHAEFVDVPVNLAMKMPEGMSFEHASTVTLGGIAMQG
                     VRRADLRMGEICVVVGAGILGLLALQMLKMSGVRVIVADLDESRLQIALQLGADKIIN
                     PSKEDAIKIVESMSGGYGADAVLFTAATSSSEPLSQAFKMCRRKGRVVLVGVVGMEIN
                     RGDIYAKELDFLISTSYGPGRYDSNYEERGMDYPYAYVRWTENRNMTEYLRMVHEGHI
                     DLKPMIAATYNIEQVTEAFDALQQPAKPIIVILSYSENLGNDVWLKYKTQDNKIVIKN
                     KSTSGDVIKVALVGAGNFATGMHLPNMKKLSSKFSLQAVMSRSGNKAKAIAEQYGAAY
                     ATANYNDILTDKDVDLVMIATRHDSHASLALQALQAGKHVFVEKPMATTQEALDAIKN
                     FYDNNSSAPLLMTGFNRRFSSFAVETKKHTDKRINPLLIYYRMNAGHIPYDNWVHEHG
                     GRMVGECCHIIDLMTFLTASKIKSVSVEQISPANDKMQSADNKAIILKYHDGSVCTIH
                     YFTNGSKQISKEYMEVNFDGNTIIMDDYKTLRGFGIKINDITESISCKGQIEELQALH
                     KCMKENKFPIELWDMVQTTEISFVL"
     gene            6242..9025
                     /locus_tag="PK736_03805"
     CDS             6242..9025
                     /locus_tag="PK736_03805"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC32549.1"
                     /translation="MKPAFNRENTFSKSISCIFILVSLLFFFSILVTQAQTIKITGQV
                     VDTTKVNTVHNAVVMVLRFSDSLMVKFTRTDVNGKFEINSLPVDTYNIVASHAQFGDV
                     AYIVIGKPNDTVVNFGKMILPAKSISLNEVVVYGYTDPIFMKGDTLVYTVDSFKVREN
                     AVVEDLLKKLPGIKVDANGKIFTQGKEVDQVLVDGDEFFGSDITVATKNLAASSVDQV
                     QVYDKKNDAQGESDKETLKVLNLKLKEDAKKGYFGKASAAGDFQKYYEGEFLFNRFKG
                     SQKISAFALTSNTPRSSLGWRDMWDYGFTDDMNMSSGDDGETYFNWNNNDNNGVPRTL
                     KTGVYFNDKILPKTKLNLSYTFTNGDVTSKSSTFSQYFLSDSSYNAEYNYQSFQETQA
                     HRANITVKQTIDSLTDLEVKSNIKINSGSKEESTMQKFSQSNSDIFRSNSIENKSQTS
                     GTDLGVEARLIRNFTKRDRKLVMSYSYNGNDADGTGYLFNEDDNFIDSTLNDTINQQK
                     LKSNKKRDNSFSVSYVEPITKKIRLDWSYDYTSSILQQNKKSFDAVNGNYSQENDLLT
                     SNYTNARTINRGSMGVTYEVKKMRVSVGSKVRQIYAIGDEQRTNYKLTQTVTNLLPYI
                     QYRYKFGENTSLQMKYNTNAKQPELFQLQPVQDNANTNNIYIGNPNLLPSYSHRFNMN
                     YYMFKPISGRNYYINGNYTLTNNSITNAIIYGDYGKTITQPVNTDANYSYGGYAGGSI
                     NFFDQKFEVNPGVSFDGSKINSIVNTNKNITRNLTTGAELELALRLDSFEFAISTSQE
                     YTQTSSTVSDRGNSNFFTRNYRASLSKDFKWKFTFETDAEFNTNTQRAQGYNVNFILW
                     NASISKKFGKGENLIASFVATDMLNQNINTYRTVEDNVISDVKNTIIGRYLLLKVVYK
                     FKNKGAAKEDDEE"
     gene            9015..9737
                     /locus_tag="PK736_03810"
     CDS             9015..9737
                     /locus_tag="PK736_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HRC32550.1"
                     /translation="MKSKLFILFILVQTIACSMFGQLTQGKIVYERKTNLYKKFKDDN
                     VTDWIKERDKIKIDYFELLFNDSLSLFRPQESELKETMWWATEKNVVYQNFNIDSRFV
                     IKKIWGEPLNILDTLYDRKWKITESKRVICGYNCRKAIWQYNDSVRIYAWYCDDIIPS
                     VGPESFYGLPGAILGLASEDGGIIYFAKSVERIDIKTTEITKPKTKDKVYTSQELKTK
                     LEKEFGKEKWGKAMIKNTFGYW"
     gene            9958..11184
                     /locus_tag="PK736_03815"
     CDS             9958..11184
                     /locus_tag="PK736_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HRC32551.1"
                     /translation="MKDKKQAAIGFIFITLLIDVIGLGIIIPVMPSLIEELIHGSLSD
                     AAKYSGWLMLSYSVMQFIFAPIIGGLSDKYGRRPVLLASLLAFGLDYLLLAFAPTIGW
                     LFLGRIIAGITGASFSTAQAYIADISSDENRAQNFGMVGAAFGLGFIIGPMLGGLLGE
                     FGSRVPFFVSAGVALLNFVYGYFILPESLSKENRRPFNWKRANPIGTFNQIKVYPIVM
                     YMMGVMFFIYLAGHSLQSTWTFIMMEKFKWTKATVGYSLGVVGLAVALVQGLLIRFIN
                     PKLGDERSIYIGFTIGGAGMLLWAFATQTWMIFAITFVYCLSGIAMPALQSIMSKNVA
                     SNAQGELQGALASTVSVTSIIGPLMMTYIFSYFSSSKAAIYFPTMPFVIAAIFFIISI
                     LGVFYTLNKFREKNVE"
     gene            complement(12137..12994)
                     /locus_tag="PK736_03820"
     CDS             complement(12137..12994)
                     /locus_tag="PK736_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32552.1"
                     /translation="MKNILAFVVASLLTINNFQAQTLVDTNKVWHVAECLNFAGCGTS
                     VMKFGGDTIIGLHQYKKIIIDKDSSWFGIYFFEAAREDTISKQIFFHTGNAEYLVYDF
                     SLQQGDTFVTNISGCYIQMVVDSVSTLTLINGEVRKIMYLNQDQWIEGIGSLVGIYYA
                     GIINCINDVYTQLICFTENDTLKYMNNFYQSCYYNTTSLGEVENENIFIAKPNPFSDY
                     TTITINQPSHGTTTVNIYNSFGERTKQYKNVSGKELKIEKGDLQSGMYFIQLLNDDKE
                     ISRGKIVIL"
     gene            complement(13049..15820)
                     /locus_tag="PK736_03825"
     CDS             complement(13049..15820)
                     /locus_tag="PK736_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRC32553.1"
                     /translation="MKKFLKIFAVVLMLFIAFLIAIPFLFKGKIIEAIKQEANKSLDA
                     KLNFGDVDLTLISSFPNLKLSLQDLSIIGNAPFDGDTLIYTKNISIKVGLYDVVSGGQ
                     TNIKSIVLDNPFINILILKDGKANYNITKESKDASTGSTESTAFKLALNSYEINNGKI
                     NYDDRSLTFRMLLYDVNHKGSGDFTQDLFLLKTQTEVKSTDVWYGGVKYLSQVQSKLL
                     ADLDMDMKNFKFTFKENELALNDLSTAFDGWLAMPKDDIDMDLKWKVNRNDFKSFMSL
                     IPGVYSSSFKDVKCTGNLAMNGFVKGTMTDTRMPGYGLNVKIDNGNFQYPSLPSAIKN
                     TIVDLKINNPDGVTDHTIIDLKKMHVEIGGDPFDAKLFVTIPVSDANIDAMLKGKINL
                     GNLKNTVPLPQGMILTGILNADMLAKGRYSAIEQKRYEDFNASGTMSVADMNYNDTKS
                     GDKYFIKQFMLTFNPKNVTLNNLEATVKNSDFKASGTIDNLIGYYVKDDLLKGSFNLT
                     ANKINVNDWMAGSATAEGQAADTSKMSAMQIPANIDFDMTTAIGKLIYDDIIMTNVKG
                     NIVARDEKLSMNNLFMNLMGGSMLMNGTYSAKNIKKPDYTFKLNLTDWDIQQTNKTFN
                     SVKKFAPITSKCNGKFSIEMDMLGNMDEHMQPQLDKLNAYGKLQSKDVTLNNLTALSK
                     IADELNMPQYKQMKLSNVNVSFDIKNGRLFVKPFEQNLAGTKSVIEGSQGLDQTIAYT
                     MKLNIPLSSMGTAAKGKAQSLLSQLSGKTGINISLPDPVPVDVIIGGTVLKPEIKTSF
                     KNTGANIVNTIKDEIKNQVYTQIDNAKEKARAEADKILKEAEAKSKALRDAGYQTAEE
                     IRKQGYAAADNVVKEAKNPLAKVAAQKLAEKMKKETDKKVEAAKREADDKANKINDEA
                     RKKADELLK"
     gene            complement(15859..16959)
                     /gene="aroC"
                     /locus_tag="PK736_03830"
     CDS             complement(15859..16959)
                     /gene="aroC"
                     /locus_tag="PK736_03830"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRC32554.1"
                     /translation="MASNSFGEIFRITTFGESHGQMIGVIIDGCPAGIFIDESEIKNQ
                     LARRRTAQSIFTSGRKEDDSFEIVSGYFENKTTGAPITFIIKNMDVKSDDYINLENTF
                     RPSHADFTYSAKYGNRDYRGGGRSSARETTLWVIAGTLANQILLQNNIRIKAYVSAIG
                     NIATQKNYQQFDFNTIDSNEVRCPDDNCATQMIEYLNDLKQNGDTTGGVITCVVQNMI
                     AGLGDPIFEKLQSKLAAAMFCINTVHGFEYGSGFAGASMNGSKHNDLFNNVNMKTKSA
                     ETTTNNSGGIQGGISNGMDIFFNVAFKPIASIKQEQVTANAYNELSSLVISGRHDACV
                     VPRAVPIVEAMTALTLLNAFLKNNAYNNLNLK"
     gene            17009..>17428
                     /locus_tag="PK736_03835"
     CDS             17009..>17428
                     /locus_tag="PK736_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32555.1"
                     /translation="MSKKRKAKPPITNLNDLYQDKDKDELKFDTELLKMQIGAETGNM
                     PVISEDTPIELQHFFLNNVMNFEKQFSEANEEAIGVLLGKKKFKDAATMNTQQLNIEF
                     DKLFDLLGRHNISVTWLPGTRKRTIYKFIVEELIHLKV"
     assembly_gap    17429..17448
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17449..>17656
                     /locus_tag="PK736_03840"
     CDS             <17449..>17656
                     /locus_tag="PK736_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32556.1"
                     /translation="MPGMMRIFPYEEFHPNHKHDCMENAIMFLQSLLKLDLKIAEYLI
                     HDELIFNNISYDKKIFLQKASLFK"
BASE COUNT         5544 a         3183 c         3325 g         5584 t
ORIGIN      
        1 ccgtaggctc gactccattt tgtaaagatg gatttcaatc catcccaatg tgcatgagac
       61 aacaaattcg cgaaccaata tgccagcaat tgtttgtgcc ccaataaaca ttcttaaacc
      121 attacaaaaa atcgtaataa ttatacacca aactcaatcc ttctcgacaa atctcgggct
      181 ccgtgctgaa tcattagaat atgaatttcg tgtttgcttg ttattctata aattatgcgg
      241 tagttgcctt caattaattc tctgatattt tcattttcaa gctccacaac taccttacct
      301 atttcaattt gattctttaa tatttctgtc ctggttcgaa ttttttcaac ttgcaattgt
      361 gcatatcgtt tggaatcttt agaaatgtaa tcataaattt ctcgcaagtc atttttagca
      421 gattgtaacc actttatttg aaccattttg ccatttccaa ttttagttct tcttcagaaa
      481 ttgttttgtc ttgttgcgat tcagcaattc tgttttccaa tttttcaata aaaattagcc
      541 tttcaattaa ttcatcaatc gaaatatttt ctgggaatga ttctatatga atttttaatt
      601 tttcttttgt tatcataatc ctatttttta caaatataca atttgttatt tgactgtttg
      661 cttatggtca gtgttttcac aaattatcac cacgattttc aagttcaaaa aatgcctaac
      721 aattcttcta accaaacaaa acttattgta catttactag accaataata attgataaac
      781 ctaaaatgaa actctacaag taatgaaaaa acaacacaaa attttattct tatttctcat
      841 acaaaatatt ttggccataa ccatatacgc tcaaggaggc acttggactt ggataagcgg
      901 gagtagtaac gcgaatgcat taggagtgtt tggcacgcaa ggaataccat ctgtaaacaa
      961 ccacccacca gccgtttatg agccatgtga atggaaggac aaacaaggta acttttggat
     1021 ttatgggggt acttataatt catattgtga cctttggaaa tttaacccaa ctactttgga
     1081 atggacttgg gttaaaggta gtggattaca aaacatgcca acctattatg gtactaaagg
     1141 agttgctaat atttccaact ctcctggagt aagaagtttt tgttctatta catgggttga
     1201 tacttcaggg aatttctgga tttttggtgg cgcaaattcg tctaacgctt tgtggaaata
     1261 tgacatagcc aataacatgt ggacgtgggt taatggcacc acaaatacca atgcttatgg
     1321 agtatacgga atcaaaggcg tgcccgcggc atcaaatgtt cccggaatta gaaaagaaac
     1381 atgttcggga tggaccgaca gcttgaataa cctttggctt tttggtggtt ttggtaatgg
     1441 cgtctctgct ttgggttacc tcgatgattt atggaaatac aatatcgcta caaatgaatg
     1501 gacatggatg gccggagatt ttggtataaa caaattggca aactatggtg tgaaaggagt
     1561 ttcgagtgcc acaaataacc ccggtgggcg catgagttat acaaagtgga aagatgctgg
     1621 aggaagcttt tggatttttg gtggctctga ctctaaagta aaaaatgatg tttggaagta
     1681 tcaacctgat attaaccaat gggtatggat gaatggtaaa agcacaaata acgattcggg
     1741 ttactataat tctttttgtg cattcgacag cacacttctt cccttttcac gctttgaaca
     1801 ccgaagttca gttacggata attgtggaaa cttctggatg tatggcggct tcagaagtgg
     1861 tgcttttcca caaaatgatc tttggttttt cgatccgcaa atattgcaat ggaaacttct
     1921 gaatggcacc aataaatttt ttcaacccgg taattatgga ttattgggcg tgccgtcagt
     1981 gaataacgca ccacctagta gaggaggagc ttccgcatgg tggtgcgatg ataacaaatt
     2041 ttatatgttt ggcggtttgt attcaatcgt taagagttat gccgatgtat gggttttcga
     2101 tcccgataca aattgtatta gtttcaactg caataacagc acctcagcca tcgcaaattt
     2161 ctcctcctcc gattctctct tctgcgaaaa gcaatgcatc gattttacag atatttcgca
     2221 aaataatcct acatcctggc aatggacttt taatggggca tcgccatcta catcaaccga
     2281 ccaaaaccca acaagtattt gttacaattc ttatggcagt tttgatgtta cgcttgttgc
     2341 atgcaatcaa tttggttgcg ataccattac aaagaataat tttattactt catacatcac
     2401 gcccttcgat agcatttggt ttgccaatga tacattgttt gcattgcctg catacagcta
     2461 tcaatggtac gctgtagggg cggggcttat tgccaatgca acgcaaaatt attatgtacc
     2521 acttacttta ggtagttact attgcaccac aaaagattcg gacggatgcg aggcaacatc
     2581 gaatgtaatt ttatacacca gtttttttga aaatgaaaat aatgaattgg caaatgccgt
     2641 atggcctaat cctgcaaacg aaatattatt tgtaaatcca caattgctaa acgctaaaaa
     2701 tatcattttc gaaatttacg atatgcaagg cagaattatt ttgcaacaaa tgttgagcaa
     2761 caatatcaac tcaattaaca taagtcaact tgccaccagc gtatatagct accgtataat
     2821 gacaatcgaa aaaataattg cgcaaggaaa atttgtaaag caaattaaaa attgaagatt
     2881 atgaattaag aattggggct gcatgagcca tggtttgtgt ccccacaaac catcatcacg
     2941 atgccgctac gatcttgcat ttggccagtt tgcatcagcc atggtttgtg tcctcacaaa
     3001 ccatcaattc gatttcactc taattaccac agattaaaat ccgtgactac aaaatactcc
     3061 gttcctccgg aactcatgct gtgtatatct ttgctgcgta agcaatagag ccgtaggctc
     3121 gactccattt tgtaaagatg gatttcaatc catcccaatg tacgtgcatc agccatgttt
     3181 tgtgacctca caaaccatca attcgatttc actctaaata ccacggatta aaatccgtgg
     3241 ctacaacata ttccgttcct acggaactca tgctgtgcgt atctttgctg cgtaagcaat
     3301 agagccgtag gctcgactcc attttgtaaa gatggatttc aatccatccc aatgtgcatc
     3361 tacaaatcaa gccgcgtcag ccatggtttg tgtctccaca aaccatggcc actttgtctc
     3421 taagaaaaac cacgatttgt aagaagtagt tgaagttctt tccaacttac atgtgttact
     3481 tctgcactgt acgcgtcagc cgtattgtgc attgctggaa cagcgatgca ccggagggct
     3541 caccagcccc cgcaataaaa tgtattttaa ttctcattca atttagttga tttcgattaa
     3601 tcctataaat tatttattct ccatcaagcg gaacttcaaa cctcaaactt cgaacttcaa
     3661 acttcgaact tcaaactaaa aacttcaaac ctcaaactta tttcaattat tctacttttg
     3721 cgccacaatt ataaagcgag taaagtgata caagcattag ttaagaaagg aaaagttata
     3781 ggcgaggtag taccggcacc ggtggtaagc gatgggtgtt tgttgataca ggtggtgaac
     3841 agttgtatat cggcaggtac agagttgacg agtgtgcaga catcgaaaaa gtcgttgata
     3901 aagcgtgcga tggaacagcc cgaagaagta aaaaaagtgc tggactttgt gcgcagcaat
     3961 ggcatagaaa aagcatacct gcgtgtaaag ggtgtgatgg atacgggcaa gcctaccggc
     4021 tattcgattg cggggcgtgt gattgctgtg ggtaaaggtg tgacaggtta tgaggctggc
     4081 gactgtgtgg ccgcggccgg tgcgggtatt gccaaccatg cggagtttgt agatgtgcct
     4141 gtaaaccttg ccatgaaaat gcccgaaggc atgagctttg aacatgcgag tacggtaaca
     4201 cttggtggca tagccatgca aggggtgcgc agagcggatt tgcgcatggg cgaaatatgt
     4261 gtggtggtag gtgcaggcat acttggtttg ctggcattgc aaatgttgaa gatgagtggt
     4321 gtgcgtgtaa tagttgccga ccttgacgaa agccgcttgc agattgcttt gcaactaggt
     4381 gcagataaaa taataaatcc atcgaaagag gatgcgataa aaattgtaga gagtatgagc
     4441 ggtggttatg gtgccgatgc ggtgttgttt actgcagcaa caagtagcag cgaaccgtta
     4501 tcgcaagcat ttaaaatgtg cagacgcaag ggtcgggtag tgcttgttgg tgtagttggt
     4561 atggaaataa atcgtggtga tatatatgct aaggaattgg attttttaat ctcgacttcg
     4621 tatggccccg gccgttacga tagcaattac gaagagcgcg gtatggatta tccttatgca
     4681 tatgtgcgct ggaccgagaa ccgcaacatg acagaatacc tgcgtatggt gcatgagggc
     4741 cacatcgatt tgaaacctat gattgctgcc acttacaata ttgagcaagt gaccgaagca
     4801 tttgatgcat tgcaacaacc tgccaaacca attatagtta tactaagtta tagcgaaaat
     4861 ttgggcaatg atgtttggtt gaaatataaa acgcaggaca ataaaattgt aataaaaaat
     4921 aaaagtacaa gtggcgatgt tataaaagtg gcattggtag gtgcaggaaa ttttgcaacc
     4981 ggtatgcact tgcccaatat gaaaaaactt agcagcaaat tttcgttaca ggcagtaatg
     5041 agtcgtagtg gtaacaaggc caaagccatt gccgaacaat atggcgcagc atatgctact
     5101 gctaactata acgatatact taccgataag gatgtagacc ttgtgatgat tgccacgcgg
     5161 cacgatagcc atgcatcgct tgcgctgcag gcattgcagg ccggtaagca tgtgtttgta
     5221 gaaaaaccaa tggccactac gcaagaagca ttggatgcga taaaaaattt ttatgataat
     5281 aattcttctg cgcctttgtt gatgacagga ttcaatcgga ggtttagttc gtttgctgta
     5341 gaaacaaaaa agcataccga taaacgcatc aatcctctgt tgatttatta tcgaatgaat
     5401 gccggacata ttccttacga caattgggta catgaacatg gtggacgtat ggtgggtgag
     5461 tgttgtcata taattgactt gatgactttt cttactgcga gcaaaataaa atcggtaagt
     5521 gtggagcaaa tatctcctgc aaacgataaa atgcagagtg ccgataacaa agcaattatt
     5581 ctaaagtatc atgatggaag tgtttgcacc attcattatt ttacaaatgg cagcaagcaa
     5641 atttcgaaag aatatatgga agtgaatttt gacggcaaca ccatcattat ggacgattac
     5701 aaaacactgc gcggctttgg aataaaaata aatgacatta cagaaagcat cagctgcaaa
     5761 ggacaaatag aagaattgca agcactacac aaatgcatga aagaaaataa attcccaatc
     5821 gaactatggg atatggtgca gacaactgag attagttttg tgctttgagg agttgagaaa
     5881 gttttgaaag ttgggatggt tgggaaagtt ttgaaagttg atagtttgaa tgcaaagcaa
     5941 tcattgccgt tcggctttag ccaacggatt tttttttgag attaataatt taaatgaaaa
     6001 attaaaaatg gaattatgtc cttcacttct aacttatcaa tgcaactctt cgataggcat
     6061 cacaacataa gtgtcgtgat tattcttttt tcttgacagt tcgagaatac tcactgcatg
     6121 cttttgattt atcctttatt cccttttgaa ttaacctcct tcgattttaa aattatgact
     6181 tctataacac ttgatcccaa taataatcat tttattgcgt tatgaaatct aaaccatcag
     6241 aatgaaaccc gcattcaaca gagaaaatac ttttagtaaa tctatatcct gcatttttat
     6301 tcttgtatca cttttatttt tttttagtat actagtaacg caggctcaaa caataaaaat
     6361 aaccgggcaa gttgttgata ctactaaagt gaataccgtg cacaatgcgg tagtaatggt
     6421 tttgcgcttt tccgattcgc ttatggtaaa atttacccgt accgatgtga atggaaaatt
     6481 cgaaattaat tctctgccgg tcgatacata caatattgta gcatcgcatg ctcaatttgg
     6541 agatgtagcg tatatagtaa taggtaaacc aaatgataca gttgtaaatt ttggcaaaat
     6601 gatattgcca gccaaatcca tttctctaaa cgaagttgtt gtatacggat ataccgatcc
     6661 catttttatg aaaggcgata cgcttgttta tactgttgat tcttttaagg tgagagaaaa
     6721 tgcggtagtt gaagatttat taaaaaaatt gcccggtata aaagtagatg ccaacggaaa
     6781 aatatttact caaggaaaag aggttgatca ggttttggta gatggcgatg aattctttgg
     6841 tagtgatata acagtagcga caaaaaatct tgcggccagt tcggtagatc aagtgcaggt
     6901 gtatgataaa aaaaatgatg ctcagggtga atcggataaa gagacattga aggtgctcaa
     6961 tttgaaattg aaagaagatg ccaagaaagg atattttgga aaggcttcgg cagcaggcga
     7021 ttttcaaaaa tattacgaag gcgaattttt gtttaaccga tttaaaggga gtcaaaaaat
     7081 ttcggcattt gcacttacaa gtaacacacc acgcagcagc cttggttggc gcgatatgtg
     7141 ggactatggc tttaccgatg atatgaatat gagtagcggt gacgatggcg aaacatattt
     7201 taactggaac aataatgata acaatggcgt tccacgcaca ttaaaaaccg gagtgtactt
     7261 caacgataag atactgccaa agacgaaatt gaatttaagt tacactttta ctaatggtga
     7321 tgttacttcc aagtcgtcca ctttctcgca atattttctc agcgattcga gttacaatgc
     7381 cgagtataat tatcaatcgt ttcaggaaac acaggcgcat agggccaata ttaccgtcaa
     7441 gcaaacaata gattcgctta ccgatttgga agtaaaatcg aacatcaaaa taaatagcgg
     7501 aagcaaagag gaaagtacaa tgcaaaaatt ttcgcaaagt aatagtgaca tttttcgcag
     7561 caatagtatt gagaacaaat cgcaaacaag tggtactgat ttgggcgtgg aggccaggct
     7621 gattcgcaat tttacaaaac gcgatcgtaa gttagtaatg tcttattcct acaatggtaa
     7681 cgatgcagat ggcaccggtt atttgttcaa tgaagatgat aattttattg attcaacctt
     7741 gaatgacacc atcaatcaac aaaaattaaa atcaaataaa aaacgtgata acagtttttc
     7801 tgtttcatat gtggaaccaa ttacaaaaaa aattcggctc gattggagtt acgattatac
     7861 ttcgagcata ttgcaacaga ataaaaaatc tttcgatgca gtgaatggga attattcgca
     7921 agagaatgat ttgcttacaa gcaactatac aaatgcacga acaattaaca ggggaagtat
     7981 gggagtgacc tatgaagtaa aaaagatgcg tgtaagtgtt ggttcgaaag tgagacagat
     8041 atatgctatt ggtgatgaac aacgtacaaa ttataaactt acacaaacag taacaaacct
     8101 attgccatac attcaatacc gatacaagtt tggcgagaat acttcattac aaatgaaata
     8161 caataccaat gcaaagcaac ctgaattgtt tcaactacag cctgttcagg ataatgcaaa
     8221 cacaaacaat atttatatag gcaatccgaa tttactgcca tcatactcac accgtttcaa
     8281 catgaactac tatatgttta aaccaatatc cggccgcaat tattatataa atggaaatta
     8341 tacactaaca aataatagta ttaccaatgc aataatttat ggcgactatg gcaaaaccat
     8401 aactcaacct gtgaacaccg atgccaatta ttcttatggt ggatatgcag gaggaagtat
     8461 taattttttc gatcaaaaat ttgaagtaaa cccgggagta agttttgatg gaagcaaaat
     8521 aaatagcata gtaaatacaa ataaaaatat tacccgcaac ctaaccaccg gtgccgaact
     8581 ggaactggca ctgcgcttag attcattcga atttgctatc agcacaagtc aggagtacac
     8641 tcaaacttcg tcaacagtaa gcgaccgtgg caactccaat ttctttaccc ggaattatag
     8701 agcttcgtta tcgaaagatt ttaaatggaa atttactttc gaaaccgatg cagagtttaa
     8761 taccaacaca caacgagctc aaggttataa tgtgaatttc attttatgga atgcatccat
     8821 ctccaaaaaa tttggcaagg gcgaaaacct tattgcttct tttgtggcaa ctgatatgct
     8881 taaccaaaat attaatactt accgaacagt ggaagacaat gttatttccg atgtgaagaa
     8941 cacaatcatt ggccgatact tattattgaa agttgtttac aagtttaaaa ataagggcgc
     9001 agcaaaagaa gacgatgaag agtaaacttt ttattttatt tatattggtt caaactattg
     9061 cttgcagcat gtttggacaa ctaacgcagg gtaaaattgt atatgaacgc aaaaccaatt
     9121 tgtacaaaaa gtttaaagac gataatgtta ctgattggat aaaagagcgc gataaaatta
     9181 aaatagatta ttttgaattg ctgttcaacg acagcttatc actctttcgt ccgcaggaaa
     9241 gtgaactaaa agaaacaatg tggtgggcta cagaaaagaa tgtagtgtat caaaatttca
     9301 atatcgattc gcgatttgta ataaaaaaaa tatggggcga gccgctcaat attttggaca
     9361 cactatacga tcgcaaatgg aaaattaccg aaagcaaacg tgtgatatgc ggttacaatt
     9421 gtcgcaaagc tatttggcaa tacaacgata gtgtacgtat atatgcatgg tattgcgatg
     9481 atattattcc atctgtagga cccgaaagtt tttatggctt gcccggtgct attcttggtt
     9541 tagcatcgga agatggtggc attatttatt ttgccaaaag tgtagaacgt atagacataa
     9601 aaaccacaga aattaccaaa ccaaaaacga aagataaagt ttacacctcg caagaattaa
     9661 aaaccaaact cgaaaaggaa tttggaaaag aaaaatgggg caaagccatg atcaaaaata
     9721 cttttgggta ttggtaagcg gtatacgtta ttctggaaaa tatttaggcc cacaacaatc
     9781 aattagaatt tagattataa gtttttataa ctgaatcatt cttcacaaat atttaataca
     9841 ttctcaatta aggattaaat catttttagt tttgcatcgc aaattattaa aatcgaaaat
     9901 cattttagta gccctgcaag aaaaacgtaa cacattgtgc ataagaataa atagaaagtg
     9961 aaagacaaaa aacaagcggc aataggattt atatttatca ccttactcat tgatgtgata
    10021 ggccttggta ttattattcc tgttatgcca tcactgattg aagaactcat acatggcagt
    10081 ttgagtgatg cagcaaagta cagtggttgg cttatgctat cgtacagtgt gatgcaattt
    10141 attttcgcgc ccatcattgg cggcctatcc gataaatatg gacgcaggcc tgtgttgctg
    10201 gcctcgttgc tggcatttgg gctcgattat ttgctgctcg cctttgcacc tacaatagga
    10261 tggttatttc tggggcgtat tattgccggc ataacgggtg caagttttag cactgcacaa
    10321 gcttacattg ccgacattag cagcgatgaa aatcgtgcac aaaattttgg aatggtgggt
    10381 gctgcatttg gactcggttt tattattggt cccatgcttg gtgggttgct tggtgagttt
    10441 ggttcacgtg ttccgttttt tgtaagtgcc ggtgttgccc tactcaattt tgtttatggt
    10501 tattttattt tgcccgagtc gctgtcgaaa gaaaaccgca gacctttcaa ttggaagcgg
    10561 gcaaatccca ttggcacttt caatcaaatt aaagtttatc ccatagtgat gtacatgatg
    10621 ggagttatgt tctttattta tttagccggg cattcgttgc aaagcacatg gacatttata
    10681 atgatggaaa aatttaagtg gacaaaagct acagtgggat attcgttagg tgtagttggt
    10741 cttgctgttg cattggtaca aggattactc attcgtttta taaacccaaa gctgggcgat
    10801 gaacgttcta tttatattgg ctttacaatt ggtggagcag gcatgttatt gtgggctttc
    10861 gccactcaaa cgtggatgat atttgccatt acttttgtgt attgccttag tggaattgca
    10921 atgcctgcat tacaaagtat aatgagtaaa aatgttgcct ccaatgcaca gggcgaattg
    10981 cagggtgcgt tagcatctac agtaagtgtt acatctataa ttggacctct tatgatgacg
    11041 tacatatttt cttatttctc gtcatccaaa gccgctatat attttcctac tatgccattt
    11101 gttattgccg ctatattttt tataattagt attctgggtg ttttttatac tttgaataaa
    11161 tttagagaaa aaaatgttga atagattttt caagaacata tttgtttgtt gattcataga
    11221 tagagccgaa acgattgata cgtaatttgt gattcaatgt aagtagacaa ttgaaagtcg
    11281 tttccaagat tgtaaatcaa taattcaaat tttcaatact tcttccattt tgtatctgaa
    11341 attggcatgc ccgacaaatt caattctaaa cattatccat tctttttaaa gaagtgccaa
    11401 caaaaataaa agagcggaaa ataaatgggc atgagtatcg atactcaaat attttagcaa
    11461 atcgttttga gcaccaagta acatatgtaa actatagtcg aagaaactca atcgaaatct
    11521 tgtagaaatt tttggcgtag ttaaagttca acccctatcg gggtttggcg gtagtggttg
    11581 tggtggcgtg cagcttctcc gcgtttcaca cggagttatt caagttaaag ccctccgggc
    11641 ttttcagcaa aaagaaaatc acaaaacgat tcatcagcaa tccaaccacg aagtggttaa
    11701 acatgaataa ccccacatgg aatgtgggga agtaacaagt ggaaaagcac gcaaccacga
    11761 agtggtttaa caaaaaatct tcgtgattct ttgcttgtac tgtatataga atttttccga
    11821 aggaaataaa tcataaattc aaattttcaa tacttctatc gttttgcatc tgaaaatgtg
    11881 atgaccgaaa aattcgattc taaactttat ccatacgttt taatgaaccc caaaataaat
    11941 taaccaactt gtaagttttc attttgcaaa aattgagtag attgattttt cgagaacatt
    12001 tttacacaat gatttataga tagagacaag aaagtttgct cttgcaaact aaccatatat
    12061 gatggattaa tcacaaaata gatttctttc atcgaaccca gaataaatga aaagtattct
    12121 aagataacct tataggttac aaaattacaa tcttacctcg agaaatttct ttatcatcat
    12181 tcaacaattg aataaagtac attccacttt gcaaatcacc tttttcaatt tttaattctt
    12241 tgcccgatac gtttttatat tgttttgttc tttctccaaa tgagttgtag atgtttactg
    12301 ttgttgttcc atgcgaaggt tggtttattg ttatggttgt ataatcgctg aacggatttg
    12361 gttttgcaat aaaaatgttt tcattttcca cttcccccaa acttgtagta ttatagtaac
    12421 aactttgata aaaattattc atatatttca atgtatcgtt ttcggtaaag caaattaact
    12481 gtgtgtaaac atcgtttata caattgatga tgccagcgta ataaattccc actaagctac
    12541 caattccttc aatccattgg tcttgattta aatacataat ttttcgtact tctccattaa
    12601 tcaaagtaag tgttgaaaca gaatcaacaa ccatttgaat atagcagcct gaaatattgg
    12661 ttacgaaagt atcgccttgt tgcagcgaaa aatcataaac taagtactcg gcattcccgg
    12721 tatggaaaaa tatttgcttg ctaattgtgt cttcccttgc tgcttcgaaa aaataaattc
    12781 caaaccaact cgaatcttta tcaattatta tttttttgta ttgatgcaaa ccaattatag
    12841 tatcgccacc aaatttcatt acagatgtgc cacaaccggc aaaatttaaa cactcggcca
    12901 catgccatac tttatttgtg tcgactaatg tttgtgcctg aaaattattt atagtcaaaa
    12961 gtgaagcgac tacaaaggca agaatgtttt tcatttttta gtttcaatta gaaattttga
    13021 agaactcgaa cttttcgaat tccgaatttt acttcaacaa ctcatcggcc ttctttcttg
    13081 cttcgtcatt tatcttattt gctttatcat cggcttcgcg ttttgcggct tctacttttt
    13141 tatcggtttc ctttttcatt ttctcagcca atttttgcgc agcaactttt gcaaggggat
    13201 tctttgcttc tttcaccaca ttgtcggcag cggcatatcc ttgcttacga atttcttcgg
    13261 cagtctgata accggcatcg cgcaatgctt tcgattttgc ttcggcttct ttcaaaattt
    13321 tatctgcttc ggctcgcgct ttttctttgg cattatctat ttgtgtatat acctgatttt
    13381 ttatttcatc ctttattgta tttacaatat tggcacctgt gtttttaaat gaggttttta
    13441 tttcgggctt caatacagta ccaccaatta ttacatcaac cggaacagga tcgggcaacg
    13501 aaatattaat tcctgtttta cccgagagtt gtgataataa cgattgtgct tttccttttg
    13561 ctgcagtgcc catagatgat aaaggaatat ttaatttcat ggtgtaagca atggtttgat
    13621 ccaatccttg cgaaccttca atcacacttt tggtgcccgc taaattttgc tcaaatggtt
    13681 ttacaaacaa gcgtccattt tttatatcga aagaaacatt cacattcgaa agtttcattt
    13741 gtttgtattg aggcatgttt aattcatcgg caattttgct tagtgcagta agattattca
    13801 acgttacatc tttggattgt agttttccgt acgcattcaa tttatctaat tgtggttgca
    13861 tatgctcatc catgtttccc agcatatcca tctctatcga aaattttcca ttacacttgc
    13921 ttgttatagg agcaaacttt tttacgctat tgaaagtttt atttgtttgc tgtatatccc
    13981 aatcggtaag gttcaactta aaagtgtagt caggtttctt aatatttttg gccgagtatg
    14041 ttccattcat caacatcgat ccgcccatca ggttcataaa caaattattc atgctcaact
    14101 tttcgtcacg cgccacaatg tttcctttta cattggtcat aataatatca tcgtaaataa
    14161 gttttccaat agcggttgtc atatcgaaat caatatttgc cggaatttgc atcgcgctca
    14221 ttttgcttgt atcggccgct tgcccttcgg cagtggcgct gcctgccatc cagtcgttca
    14281 catttatttt atttgccgtc aaattgaaag agcctttgag caaatcgtcc tttacataat
    14341 atccaataag attatcaata gttcccgaag ctttaaaatc actattctta actgtggcct
    14401 ccagattatt gagcgtgaca ttttttggat tgaatgtgag cataaattgt ttgatgaaat
    14461 atttatcgcc cgacttagta tcgttataat tcatatcggc aacggacatg gtaccgcttg
    14521 cattaaaatc ttcgtaacgt ttttgttcaa tggcagagta acgaccttta gccagcatat
    14581 cggcatttaa tattcctgta agtatcatgc cttggggtaa tggaacagta tttttcaaat
    14641 tgccgagatt tatttttccc ttcaacattg catcgatatt ggcatcgctt acaggaatgg
    14701 ttacaaataa ttttgcatcg aaagggtcgc caccaatttc gacatgcatt tttttcaaat
    14761 caataattgt atgatctgtt acaccatcgg gattatttat tttcaaatcg acaattgtgt
    14821 tttttatagc cgaaggcaac gatggatatt gaaaatttcc gttatcaatt tttacattca
    14881 acccataacc cggcattcgg gtgtcggtca tggtaccttt tacaaaacca ttcattgcca
    14941 aattgccggt gcacttcaca tctttaaacg aggaggaata cacaccggga attaaactca
    15001 tgaagctttt aaaatcattt cggtttacct tccatttcaa atccatatca atatcatcct
    15061 taggcatagc cagccaacca tcgaatgctg ttgatagatc attcagtgcc aattcatttt
    15121 ccttgaaagt aaatttgaag ttcttcatat ccatgtcgag gtcagccaaa agtttcgatt
    15181 gcacttggct taaatatttc acacctccat accacacatc agttgattta acttcggttt
    15241 gtgtttttag caaaaataaa tcctgagtaa aatctcccga gcctttatgg ttcacatcat
    15301 aaagcaacat gcgaaaagta agactgcggt cgtcataatt tattttacca ttatttattt
    15361 catagctatt aagtgcaagt ttaaatgccg ttgattctgt gctaccggta ctagcatctt
    15421 tgctctcttt ggtaatattg tagttggctt ttccatcttt caatatgaga atgtttataa
    15481 atggattatc caacacaatc gattttatat ttgtttgtcc acccgataca acatcataca
    15541 aaccaacttt aatcgaaatg ttcttggtat atatcaaagt atcgccatcg aaaggggcat
    15601 tgccaataat tgacaaatct tgtaaactca atttcaaatt aggaaatgac gatatcaaag
    15661 taagatcaac atcaccaaaa tttagctttg catcaaggct tttatttgcc tcctgtttaa
    15721 tagcctcaat tatttttcct ttaaataaaa atgggatggc aattaagaat gcgataaaaa
    15781 gcattagaac gactgcgaaa atttttaaaa actttttcat ttatttttct ttgtttataa
    15841 tttatctgaa gattggattt attttaaatt caaattgttg taagcgttat tttttaaaaa
    15901 tgcgtttagc aacgtgagtg ccgtcatggc ttctacaata ggaactgcac gtggcaccac
    15961 acaggcatcg tgccttcctg aaataaccaa tgatgacaat tcgttatatg catttgctgt
    16021 tacttgttcc tgttttattg atgcaatagg tttaaatgct acattaaaaa atatatccat
    16081 gccattgctt attccacctt gtatgcctcc ggaattattt gtggtagttt cagcgctttt
    16141 agttttcatg tttacgttat taaataaatc gttatgctta cttccattca tagatgcacc
    16201 ggcaaaccca cttccatatt caaacccatg aacggtgttg atgcaaaaca tagctgcggc
    16261 aagtttcgat tgaagctttt cgaaaatggg atcgcccaaa ccggcaatca tattttgcac
    16321 tacacaagta atcacgccac ctgtggtatc accattttgc tttaagtcat ttaagtattc
    16381 tatcatttgt gttgcacaat tatcatcagg acaacgtact tcattactat caattgtatt
    16441 aaaatcaaat tgctgataat ttttttgtgt ggcaatattt ccaatagcag aaacataggc
    16501 cttgatgcga atattgtttt gtagcaaaat ttgatttgcc agtgttccgg caattaccca
    16561 cagtgtggtt tcgcgtgccg atgaacgacc accaccacga taatcgcgat tgccatattt
    16621 tgcggagtaa gtaaaatcgg catgcgatgg acgaaaagta ttttcaagat taatgtagtc
    16681 gtccgatttc acatccatgt ttttaataat aaaagtgata ggggcaccgg tagttttgtt
    16741 ttcaaaatat cctgatacaa tttcaaagct atcatcttct ttacgtccgc ttgtaaaaat
    16801 tgattgtgcg gttcttcgcc ttgccaattg attttttatt tctgactcat caataaatat
    16861 tccggccggg cagccatcta taattacacc aatcatttga ccatggcttt cgccaaaagt
    16921 tgtaatgcgg aatatttcgc caaaagaatt tgatgccatg tatttttttt gaaccacaaa
    16981 aatagtttat attgcagaaa aaaagtgaat gtcgaaaaaa agaaaagcca agccaccgat
    17041 aacaaactta aatgacttgt atcaagacaa agacaaagac gaattgaagt tcgatacgga
    17101 gctattgaaa atgcagatag gagccgaaac cgggaatatg ccggttattt ccgaagatac
    17161 accaatcgaa ttacaacatt tttttctcaa caatgtaatg aacttcgaaa agcagtttag
    17221 tgaagccaat gaagaagcga taggggtatt attgggcaag aaaaaattta aggatgctgc
    17281 cacaatgaat actcaacaat tgaatattga atttgacaag ttattcgact tgcttggtcg
    17341 tcataatatt tccgttacat ggttgcccgg tacccgcaag cgaactatct ataaatttat
    17401 tgtagaagaa ctaattcacc tgaaggtgnn nnnnnnnnnn nnnnnnnnat atgccaggta
    17461 tgatgcgcat ttttccatac gaagaatttc atcccaatca taagcacgat tgcatggaga
    17521 atgcaattat gtttttacaa tcgcttttga aattagattt gaaaattgca gaatatctga
    17581 tacacgatga attaattttc aataatattt cgtacgacaa aaaaatattt ttgcaaaaag
    17641 catcattgtt taaaga
//
LOCUS       DAORQA010000031        16495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_907_length_16495_cov_15.358455, whole genome shotgun sequence.
ACCESSION   DAORQA010000031 DAORQA010000000
VERSION     DAORQA010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16495
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_907_length_16495_cov_15.358455"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..159)
                     /locus_tag="PK736_03845"
     tRNA            complement(86..159)
                     /locus_tag="PK736_03845"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(123..125),aa:Arg,seq:acg)
     gene            305..1849
                     /locus_tag="PK736_03850"
     CDS             305..1849
                     /locus_tag="PK736_03850"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRC32557.1"
                     /translation="MANNEDLFKNIISHCKEYGFIFPSSEIYDGLSAVYDYGQYGALL
                     KNNIREYWWRSMVQLNENIVGIDAAIFMHPTTWKASGHVDAFSDPLIDNKDSKKRYRA
                     DVLIEEHVAKIEAKIDKEVEKAQARFGETFDAGMYRTTNGRVLEYQKKADEINGRFKN
                     ALEQNDLAEVKQIIVDCEIVCPVSGSKNWTDVRQFNLMFSTSMGAVSEESNIIYLRPE
                     TAQGIFVNFLNVQKTGRMKIPFGIAQTGKAFRNEIVARQFIFRMREFEQMEMQFFVKP
                     GTEMEWYNKWKATRLNWHKALGISEKKYRFHDHMKLAHYANAACDIEFEFPFGFKELE
                     GIHSRTDFDLKSHEKFSGKKLQYFDSEDNENYVPYVIETSIGLDRMFLAILANSLVEE
                     TLEDGSTRTVLRIPSPLAPIKVAVLPLLKKDGLPEKARQIMDTLKLDFMCFYEEKDAI
                     GKRYRRQDAIGTPFCITIDHQTLEDNTVTLRQRDSMEQQRLNVDELSKIISEEVDIKN
                     LLKKLM"
     gene            1877..2596
                     /locus_tag="PK736_03855"
     CDS             1877..2596
                     /locus_tag="PK736_03855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009415186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRC32558.1"
                     /translation="MLGNIPIEHILFLDIETVPLQASFDELNEVQRKLWLHKASFLKK
                     DENQTDEEFYSRAGIYAEFGKIVCISCGFITTHKGERQFRTKSFFNDDEQIILAEFAK
                     LLNIHFSRYESLLCAHNGKEFDFPFIARRMIINKIAIPFALDIASKKPWEVPHLDTME
                     LWKFGDYKNYTPLNVLANVLGIESPKNDIDGSMIHQIYWQEKDLQRIAKYCTKDVITV
                     AQILLWFKGLPYLNADEIILS"
     gene            2768..6457
                     /locus_tag="PK736_03860"
     CDS             2768..6457
                     /locus_tag="PK736_03860"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC32559.1"
                     /translation="MHICSKNISLVLLLLIFTNKHNLMFAQKIEHEANTISAQQYLLD
                     YEKEITQDPATGTVPRERLQAGLQQILQQQQAKVQGAIPTMVWKERGPKNLPGRIRCL
                     LLDPNDFTNKRVFAGSVAGGLWRCNDITATTTAWTPVNDFFANLAVTCIAYNPSNTPT
                     IYFGTGEGWLNTEAVRGDGIFKSTDGGVTWTQLASTTGNASFQYIQKIAVATNGDVYA
                     GTIAGLQKSTDGGLTWTQVLGAGNGSVSSAIADIEIATNGDIYVSCGINTQDGVYKSN
                     SGNVGSFTKINTGTNNLPSVGLFRTELAISPTDPLFIYALPAKFSTGKYRLDSIFVSG
                     DGGASWTKSSLPIDSNPNLYGLTDISLAQATYTNTVTVDPNNKTTVFVGGMNLFKSND
                     GGVTWLQISEYTGLAGYSVVHDCQHQILFQTGNSNIVLFANDGGVYRSIDATSNTPNI
                     APRFENLNTTLSYSCAVDPSKGDMRIWSGTENNMNFFLNNTNIGNATKISHFGPGVTP
                     GINGGHFNIDYNNDSIHYTSFTAIRGNALFSYFYRSLDAGVTYSFIGQKPTNVPIGIS
                     TYDSLGKNFFVGNALVTSGQYFVNDNSSMIPGAFKTLTAAHVGGGKIRVMSASPNNAN
                     TIYFGMSNGRIIKALNANTTPVSSFSTPTTGNPSCIVVEKGNENHIIVTYSNYGMGSV
                     WETLNGGTTWTNIEGNLPDMPVRWGIFNPANSSQFILATELGVWTTDLLNGVATNWGA
                     TNNGLANVRTDMICSRPSDSLIIAATHGRGLFSCDVFSSMSVNFSADKKIRYTNKDIQ
                     FTDASVKATSWLWNFGDATTSPLQNPIHQYTAPGKYNVTLTINGGAMSEMKTNFIHIL
                     PNRVAPYLYANGGDFETNPLDFGTDDENGIPWELGNSAVLGKNGVLSGTNAWVTGLAI
                     PTYGSENDMSLMTPNFDLTGSAIFNLEFYAKYKTENGYDGFRVEYSLDKGDNWTYLGN
                     YTPTIWYNEQNNVQNTAFPLNQPYFSGDTITQYTRCFWGITPLALNLNVAFRMHFKSD
                     ASTNKAGVAIDNFQIFIPNPLPIEWLTFTGMHYDKYNRLRWAVADEAHVVHFAVERSK
                     DGITFEPIAQLVPNKKFVQTYDDYSLTEQVYYYRIRTLNESGRESYSPVISIANKQKE
                     TAQLTIVPNPSDDVIFIDGAELDDQAILQIFTAEGKQIISRNFDDVEQTNAAISTKKM
                     NLKPGIYFITITNSIGRFAGKFIVK"
     gene            complement(6465..7337)
                     /locus_tag="PK736_03865"
     CDS             complement(6465..7337)
                     /locus_tag="PK736_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HRC32560.1"
                     /translation="MKLYQFFICVIMIALLCYCGFCTKGNNIFTSQHVLPGNKFINAT
                     GNNIATRFLLPEKAVRVIHDKNSFADYLSRLPLKKNSAPVYLYNGNLKANQDAHIAVV
                     DMETGNKNLQQCADAVMRLRAEYLFSTKQMEKISFNFTSGFVCNYSKWQQGYRVIVNG
                     NNCSWQLQNKVDSSYSSFLQYMELVFNYAGTLSLTKQLKPKSIRQINSGDVFIKGGSP
                     GHTVIVVDVAQDKKTGNRYFLLAQSYMPAQQIQVLKNPQNNNLSPWYSVADLVNDVET
                     PEWTFAINDLKTFD"
     gene            complement(7402..8016)
                     /locus_tag="PK736_03870"
     CDS             complement(7402..8016)
                     /locus_tag="PK736_03870"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HRC32561.1"
                     /translation="MKSTGLLIFYLLTFITSFDLCGQQSYADSINDYRKKYIDELLSE
                     PRKPVTKEQATLLRFYQPDEKYRVSAEVVLLNNIDTIQFATSSGVKKTYTKYARATFI
                     IDTIKYTLTIFQSPTLKLKEGLQNYLFLPFKDATNGEQTYEGGRYIDCTTDDIIEGKM
                     VLDFNKCYNPYCAYSGGFACPVPPRENFIVAFIPAGEQKPAFEH"
     gene            8136..9833
                     /locus_tag="PK736_03875"
     CDS             8136..9833
                     /locus_tag="PK736_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRC32562.1"
                     /translation="MDSFSPISIILFNNDGSVAAANYNVSIIFKDKENNQLQNIKQLE
                     NNIAGITNFFNDYTKTTKDKPNHISNIFTFSDASKLNHYQLTFSALKTQSDNKFLALI
                     YKINNDDIANMSTWNEEQFQAFINSAGHYIYTLDLNLCITSFNKSFKKVFFDIFKHEI
                     KIGENPDDFVPPKISNSWSDHYLKVISGEQVSFPYAIGNGYFFVEMFPIKIDGKISGI
                     SVFSEDKTIEQTLINSLKESKFIHEFAMDVSDSGFWDWNLETNEVVYSDIWKSMLGYA
                     PHEIKNTYFSWELLVHPDDLPNALTEVKKHINGETKTYSVQKRMLCKDGSYKWILAKG
                     RIVTYDEKGKPKRFIGVHIDVDEVYNTRQELKNKNLQLEKIAFINAHKIRKPLANIQG
                     LTSIMKDKLVDENELNNYLTMLLESINELNEQTNALNNTINKEEIKALADSRKALNSI
                     RNILVLDDDPINNYIAIKMIQKTGVSCVSFTKPSEAFEYLKASNDVDLILLDLNMPVY
                     NGWEFLDNLEKAQINTPVIIVSSSSNQKDKLRAKTYKNVCDYWIKPLKVELLKNLIS"
     gene            9851..10510
                     /locus_tag="PK736_03880"
     CDS             9851..10510
                     /locus_tag="PK736_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRC32563.1"
                     /translation="MSQSIQKKLRIHEGLDITLLNAPTTYFKTVGELPSGVTTSFSLK
                     KKTGYVHLFVANKSELEKYFPIICKLLKPEGLLWISFLKKKKNASTDLTRDTGWECLT
                     QLPMKWITLISFDDNWSAFLMRNVPPEKMTLNEILHNHETNKSEKKLQPKELILPMDL
                     QQLLLKNKSAKSFFDSLSYTNRKEYVMWVDSAKRDETKSDRLRKTIEKLNAGLKNPSA
                     K"
     gene            complement(10525..10965)
                     /locus_tag="PK736_03885"
     CDS             complement(10525..10965)
                     /locus_tag="PK736_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRC32564.1"
                     /translation="MTVQLLKELFERDLNKMIAEINSYQNEKQMWELRGDVLNSGGNL
                     CLHIVGNLNHFIGATLGNTGYIRERDAEFNSKNIARQLIVMQLNQTIVMVKNTFDNLK
                     DDDLAKIYPINKWEKEWTTLQLLLHLYSHLNYHLGQLNYHRRMV"
     gene            11156..12889
                     /locus_tag="PK736_03890"
     CDS             11156..12889
                     /locus_tag="PK736_03890"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRC32565.1"
                     /translation="MALSRVWSYIILFSIIVACYHYFNGDKNIFNDLVTEKKLKENES
                     FSNQNMAIPFSLSATNDSIVNKLTSKMKAANFNITEDLLKANYIVTDDNDDDKWRNLL
                     KQKLEAVTIINFKNINNNLKTETDSAAAIYVHTLNDADKTLLDTLISKSHLHTTENIL
                     AAKYVVTSSTNTDSLSKTFLLRTERIKMVSAEKLFYYLPKNIDGIFETGKFAITLVLG
                     LIGTLALFMGLLSIAEHAGGINVISRFMSPFLSKLFPGVPKNHPSIGHMVMNFSANIL
                     GLDNAATPFGLKAMQSLQDINPNKDKATDAQIMFLALHASGLTLIPVSIIAVRAANGS
                     MSPNEIFIPLILSTFVCSLTAMLITSFKQRINILQPLIFFPLFIITSLLTVFVWYLKS
                     LPAFALEQFSDSFSNGAILFVFLIIVAGGMYKRINIFDAFVEGAKGGFETAVRIIPYM
                     VGMLVAVSLLRTSGAFDLVINPIKTFIAFTGMDTQWAEGLPTALLRPFSAGGARGFMI
                     DAMRTYGPDSFIGKMVCVLQGSSETTFYVVALYFGSVAIKDTRYSIPVMLLADLAGII
                     FSVCICYYLWS"
     gene            complement(12912..13553)
                     /locus_tag="PK736_03895"
     CDS             complement(12912..13553)
                     /locus_tag="PK736_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32566.1"
                     /translation="MDEFLRTGHAIVGTLVLVLGFLQIVLKKGGKYHRMMGVTYVLCM
                     TVVISSGIVIGSVMIGFIGAFGYYFCLTGFLFAHGKSNLKTSVQKGVVIAGALLNLYM
                     LYFAIETFMSGDINSGIIFSVFTFIFFATSIPDVMQYVLLRPVKKMYNHKMNWYFEHF
                     KRMYISYIMATTAFTVIQNIFPIAIMNWLVPTALGTIFIFISVRQQKAKMKIV"
     gene            13635..15680
                     /locus_tag="PK736_03900"
     CDS             13635..15680
                     /locus_tag="PK736_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRC32567.1"
                     /translation="MSKHFPKFILSLVLLINIFSAKAQIVDPIKWSFKVEQKNADEAT
                     LLFTAKIDEHWHVFSQFHTGDFGLPTVFDIKENKKFYKLIGKVTEPTPDEEFEPITKT
                     TLKYYQKSVTFSQKIQIICKAPFKVKGMLNYQACNDESCLAPTDVEIVCQVNKDSKEE
                     TCDVKSVNDDTTNTGSILIEEKDTTTNVAVVAPPTKLPPSSDPSILEKECSENVDENY
                     SAGSLLGTFILSFLGGLLALLTPCVFPMIPMTVSFFTKRSPTRAKGISNAIIYASSII
                     AIYVSLGLLVTLIAGPSALNEMASNSFFNLLFFVIFIIFALSFFGAFEIVLPSSWVNK
                     ADSQSDKGGLLGIFFMAFTLSLVSFSCTGPIIGTLLVEAAQSGSVIGPAVGMFGFSLA
                     LALPFALFAMFPSWLNTLPKSGGWLNSVKVCLGFIELALAMKFLSNVDLAYHWGILRR
                     ETFLAIWIVIFGLMGLYLIGKLKFSHDSDLPFISTPRLVFAILTFTFTMYLIPGLWGA
                     PLRIISGFPPPDFYKEWITGTHECPHNLNCFHDYDEGMAYAKAQNKPVMIDFTGWACV
                     NCRKMEDNVWVDPKILKRISENYVLISLYVDDKKTLDGGVQKSPYSEKQVKTTGQKWS
                     DLQTVKYLSNTQPYYVLLDHNERILAKPRGYTPDVETYSKFLDEGVCRFEKRKSASL"
     gene            complement(16130..>16495)
                     /locus_tag="PK736_03905"
     CDS             complement(16130..>16495)
                     /locus_tag="PK736_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC32568.1"
                     /translation="VDVSFSPATTGFVSCMYSVTSFLKLNYEIKYVGKQHLDNTQNEA
                     KMLPQYLVNGASVIFEKEFKSIGVIQLNFRINNLLDKRYVSNGYTYGYIYGNERITEN
                     FYFPQAGRNFVMQFVWKMN"
BASE COUNT         5401 a         3045 c         3007 g         5042 t
ORIGIN      
        1 cgaagaaact acaataatgt gcacatattt ataataaaac acaaaaaagc cccaatcaat
       61 aaagactgag gcttttgttt tgcagtggtc tcgaagggag tcgaaccccc aaccttctga
      121 ttcgtagtca gatgctctat ccagttaagc tacgaaaccg acaaacaatt gctcttggga
      181 agcgggcgca aaagtaattt ttatttccaa aaaggaaaac taccagcgaa ttattttcat
      241 aaataagtta aattctgttt aatgttcatt tttgaaatcc gtattttcgt tatttctttg
      301 ccgcatggca aacaacgaag atttattcaa aaacattatt tcccattgca aagagtatgg
      361 gtttattttt ccatcgagcg aaatttacga tgggcttagc gcagtatacg attacggcca
      421 atacggggca ttattaaaaa acaatattcg cgagtattgg tggcgcagca tggttcagct
      481 aaacgaaaat attgtgggta ttgatgccgc tatttttatg caccccacca catggaaggc
      541 aagcggacat gttgacgcat tcagcgaccc acttatcgac aataaagatt cgaaaaaacg
      601 ataccgtgcc gatgtgctga tagaagagca cgtagccaaa atagaagcca agatagataa
      661 agaagtagaa aaagcacaag caaggtttgg cgaaaccttc gatgcgggta tgtatcgcac
      721 cacaaatggc cgggtattgg aatatcaaaa aaaggctgat gaaataaatg ggcgtttcaa
      781 aaatgctttg gaacaaaatg accttgcaga agtaaagcaa attattgtcg attgcgaaat
      841 tgtatgccct gtttcgggct ccaaaaactg gacagacgtt cggcagttca atttaatgtt
      901 cagcacttcg atgggggcag tgagcgaaga gtcgaatata atttatctgc gccccgaaac
      961 agcgcaaggt atttttgtaa atttccttaa tgtgcaaaaa accggaagga tgaaaattcc
     1021 ttttggcata gcccaaacag gaaaggcatt tcgtaacgag attgtagcac gtcagtttat
     1081 ttttcgcatg cgcgaatttg agcaaatgga gatgcaattt tttgtgaaac ccggcaccga
     1141 aatggaatgg tataataagt ggaaagccac gcgcctcaac tggcacaagg ctctgggaat
     1201 tagcgaaaag aaatatcgct ttcacgatca tatgaaactg gcacattatg ccaatgctgc
     1261 ttgcgatatc gaattcgaat ttccttttgg attcaaagaa ctggaaggca tacattcacg
     1321 caccgatttc gatttaaaaa gtcacgaaaa attttcgggc aaaaaacttc aatatttcga
     1381 ttcggaagat aatgaaaact atgtgcctta tgtgattgaa acttcgattg gtttagacag
     1441 gatgttttta gcaatacttg caaatagcct tgttgaagaa actcttgaag atggttctac
     1501 ccgaactgta cttcgtatac catcaccgtt agcgccaata aaagtggcgg tgttgccatt
     1561 gttaaaaaaa gatggattac ccgaaaaagc caggcaaata atggacacat tgaaattgga
     1621 ttttatgtgt ttttacgaag aaaaagatgc tattggcaaa cgataccgca ggcaagatgc
     1681 tataggcacg ccattctgca tcactataga ccaccaaaca ctggaagaca acacagtaac
     1741 tttgcgccag cgcgatagta tggaacagca acggctgaat gtagatgagt tgtcaaaaat
     1801 aatttcggag gaagttgata ttaaaaactt attgaaaaaa ttaatgtaat aattttattg
     1861 caaaaaacct aatgttatgc ttggcaatat tccgatagaa catatcctgt ttttggacat
     1921 cgaaacggtg cctttgcaag caagtttcga tgagctgaat gaggtacaaa gaaaattgtg
     1981 gttgcataaa gcatcgtttt taaaaaaaga tgaaaatcaa actgacgaag agttttattc
     2041 acgtgcggga atatatgcgg agtttggcaa aatagtttgc attagctgcg gttttataac
     2101 aacgcataaa ggtgaacgac aatttcgcac aaaatcattc tttaatgatg acgaacaaat
     2161 tatacttgcc gaatttgcca agttgctcaa cattcatttt tcacgatacg agagtttatt
     2221 gtgtgcccac aatggaaaag aattcgattt cccttttatc gccaggcgca tgataataaa
     2281 taaaattgca attccttttg ctttggatat cgcctcaaaa aaaccctggg aagttccaca
     2341 ccttgacact atggaattgt ggaaatttgg cgattacaaa aactacactc ccctcaatgt
     2401 tttggcaaat gttttaggaa ttgaaagccc caagaatgac atagatggaa gtatgataca
     2461 tcaaatttat tggcaagaaa aagacctgca acgaatagcc aaatattgta caaaagatgt
     2521 gattacagtg gcacaaattt tactgtggtt taaaggcctc ccgtatctaa atgccgatga
     2581 aataatactt tcatagtctt tttaggccca ttttttgaaa aaacaatccc gcttaccaac
     2641 ttaaatcaat atcttaccaa cccaatcaga ggtttagcct attcggcttt ttttatacat
     2701 attaaggtac tatttttgtc ttcctgttca gcaattcgtt ttaaggcgaa gaatacctta
     2761 taaaaaaatg catatctgtt ctaaaaacat atcactggta ttgttgctgc ttattttcac
     2821 caataagcat aatctgatgt ttgcacagaa aattgaacac gaagccaaca ccatatcagc
     2881 acaacaatat cttcttgatt acgaaaaaga aataacacaa gatcctgcaa ccggaactgt
     2941 gccgcgcgaa agacttcaag ccgggctaca acaaatttta caacagcaac aagcgaaagt
     3001 gcaaggtgcc attccaacta tggtatggaa agaacggggc cccaaaaatt tacccggacg
     3061 tatacggtgt ttgttactag acccaaacga ttttactaac aaacgagtat ttgcaggaag
     3121 tgttgccgga ggtttatggc gttgcaatga tatcacagcc actacaacag catggacacc
     3181 ggtgaatgat ttttttgcta acctggcagt tacctgtata gcatacaacc cttcaaatac
     3241 tcctacaatt tattttggca ctggtgaggg ctggcttaat accgaagctg tgcgtggcga
     3301 tggcatattt aaatcgaccg atggcggtgt aacctggaca caattggctt ccacaacagg
     3361 aaatgccagt tttcaataca tccaaaaaat agcagttgca actaatggcg atgtgtatgc
     3421 gggtacaata gcaggtcttc aaaagtcgac agatggcggc cttacatgga cacaggtgtt
     3481 gggagcaggt aatggaagtg ttagcagtgc aatagccgat attgaaattg caactaatgg
     3541 cgatatctac gtttcttgcg gaatcaatac tcaagatggg gtttacaaat ctaattcggg
     3601 aaatgtgggc tccttcacaa aaataaacac tggaacaaac aaccttcctt ctgtgggtct
     3661 ttttcgaacc gagttggcca tatcacctac cgatccttta tttatatatg ctttaccagc
     3721 aaaattctca acaggaaaat atagattaga ctcaatattt gtatcaggcg atggcggggc
     3781 aagttggaca aaatcatctt tgccgattga tagcaatcca aatttgtacg gattaactga
     3841 tatttcgtta gctcaagcta cgtatacaaa taccgttact gttgacccga ataacaagac
     3901 aacagtattt gtaggcggca tgaacttgtt taaatctaat gatggtggtg ttacttggtt
     3961 acaaatttca gaatatacgg ggctagcagg atattcagtg gtacatgatt gtcagcatca
     4021 aatacttttt caaacaggca atagcaatat tgttctgttt gctaacgatg gcggggtgta
     4081 tcgttctatt gatgcaactt caaatactcc aaatattgca cctcgtttcg aaaatttgaa
     4141 tacaacactt agctacagtt gtgcagttga tccatctaaa ggcgatatgc gaatttggag
     4201 cggcacagaa aataatatga actttttttt aaacaatacc aacataggta atgcgactaa
     4261 aatcagccac tttgggcctg gtgtaactcc cggaattaac ggaggacatt tcaatattga
     4321 ttataataac gacagcattc attatacatc cttcacagca ataagaggca atgctctttt
     4381 ttcttatttt tatagatcgt tggatgctgg agtaacttac agctttattg gacaaaaacc
     4441 cacaaatgtt ccaatcggta tttctactta cgattctttg ggcaaaaatt tttttgttgg
     4501 taatgcatta gtaacatctg gccagtattt tgtaaatgac aactcttcaa tgattccagg
     4561 tgctttcaaa acattgactg cagctcacgt tggtggcggt aagattaggg taatgtccgc
     4621 atcacctaat aatgcaaata ctatttattt cggaatgagc aatggaagaa taataaaagc
     4681 attaaatgca aacacaaccc cagtgtcttc tttttcaact ccaacaacag gaaatccatc
     4741 atgtatagta gttgaaaaag gtaacgaaaa ccacatcata gtaacatact ccaattacgg
     4801 aatgggcagc gtttgggaaa cattaaatgg aggcaccaca tggactaata ttgaaggcaa
     4861 cttgcccgat atgccagtgc ggtggggaat atttaatccg gcaaattcat cgcaatttat
     4921 tctggctacg gaacttggtg tatggacaac agacttatta aatggcgtgg ccaccaattg
     4981 gggtgcaact aataatggtc tcgccaacgt acgtaccgat atgatttgtt cgcgcccatc
     5041 ggacagctta ataattgctg ctacacatgg ccgtggatta ttttcgtgcg atgtgtttag
     5101 ctctatgtcg gtaaattttt cggctgacaa aaaaatacgc tacacaaaca aagacattca
     5161 gtttaccgat gcatcagtaa aagctacatc gtggctatgg aactttggcg atgcaactac
     5221 ttctcctttg caaaatccta ttcaccaata taccgctccg ggtaagtaca atgtaaccct
     5281 tactataaac ggaggtgcaa tgagtgaaat gaaaacaaat ttcattcata tattgccaaa
     5341 cagagttgcc ccatacctgt atgcaaatgg tggcgatttc gaaactaatc ctttggattt
     5401 tggcaccgat gatgaaaatg gaattccttg ggaattgggc aattcggcag tgctgggtaa
     5461 aaatggtgtg ttatcaggga ctaatgcctg ggtaacaggt ttggcgattc ctacttatgg
     5521 ttcggagaat gatatgagcc tgatgacacc aaacttcgac ttgacaggaa gtgcgatttt
     5581 taatcttgaa ttttacgcaa agtataaaac agaaaatggg tatgatggtt ttcgcgttga
     5641 gtattcttta gataaaggag ataactggac ttaccttggc aactatactc ccactatttg
     5701 gtacaacgaa caaaacaatg ttcaaaacac cgctttccct ttgaatcaac cttatttttc
     5761 gggcgatacc attacacaat atacacgttg cttttggggt atcacaccat tagcattaaa
     5821 tcttaatgta gcgttccgca tgcattttaa aagtgatgca tctaccaaca aagccggtgt
     5881 agctattgat aatttccaaa tatttattcc caatccgcta ccaattgaat ggcttacgtt
     5941 tactggtatg cactatgata aatacaaccg gctacgttgg gcagtggctg atgaagccca
     6001 tgtggtacac tttgcagtgg agcgttctaa agatggaata acattcgagc caattgcaca
     6061 attagtgccc aacaaaaaat ttgttcagac ttatgatgat tattccttaa ccgagcaagt
     6121 atactattac cgtatacgca cgttaaatga aagcggcaga gaaagttaca gccccgttat
     6181 ttccattgca aacaaacaaa aggaaaccgc acagttaact atagtaccta atccaagcga
     6241 tgatgtaatt tttattgacg gtgctgaact ggatgatcag gctattttgc aaatatttac
     6301 tgcagaagga aagcaaataa tatcgcgaaa ttttgacgat gtggaacaaa caaatgcagc
     6361 catttctacc aaaaaaatga atttgaaacc cggaatttat tttatcacta tcacaaatag
     6421 tattggccgc tttgctggta agtttatagt aaagtaattg tttttcaatc aaaagttttc
     6481 aaatcattta ttgcgaaagt ccattcagga gtttcaacat catttactaa atcggcaacg
     6541 ctataccacg ggctgaggtt gttattttgt ggatttttca aaacttgaat ttgttgggcg
     6601 ggcatataac tttgtgccaa taaaaaataa cggtttcccg tttttttatc ttgagcaaca
     6661 tcaacaacaa ttacggtatg gccggggctt ccacctttta taaaaacatc acccgaattt
     6721 atttgccgta tacttttagg cttcaattgc tttgtaagcg acaatgtgcc ggcataatta
     6781 aaaaccaatt ccatgtattg caaaaagcta gaataagaac tatcgacttt gttttgcaat
     6841 tgccatgagc aattattgcc attcacaatt acacggtatc cttgttgcca tttcgaataa
     6901 ttacaaacaa aaccacttgt aaaattgaac gatatttttt ccatctgttt ggtggaaaac
     6961 aaatattcgg cccgtaagcg cattactgca tcggcacact gctgcaagtt tttatttcct
     7021 gtttccatat caacaactgc aatgtgtgca tcttgatttg ctttcaaatt gccattgtat
     7081 aaataaaccg gagcgctatt tttcttcaaa ggcagccgac ttaaataatc tgcaaaagaa
     7141 tttttatcat gaataactct tactgctttc tcaggcaaca aaaaccgcgt tgcaatattg
     7201 ttgcctgttg cattaataaa tttattcccg ggtaatacat gttgtgacgt aaatatatta
     7261 ttgcctttag tacagaatcc acaatagcat aacaaagcaa tcataatcac acaaataaag
     7321 aattgatata atttcatttt ggagtattac aaattggtgt aaatacttaa actacacaag
     7381 cgaaatttta ttgcaagatt ttcaatgttc aaatgccggc ttttgctcgc ctgctggtat
     7441 aaacgcaaca ataaaatttt cgcgaggtgg caccggacaa gcgaaaccac cactataagc
     7501 acaatatgga ttgtaacatt tattaaaatc taaaaccatt ttaccttcaa taatatcatc
     7561 tgttgtgcaa tcaatatatc gcccgccttc atatgtttgc tcaccattgg tggcatcttt
     7621 aaatggcaga aacaaataat tttgaagccc ttctttcaat ttcaaagttg ggctttgaaa
     7681 tatagtaagt gtatatttta ttgtatcgat gataaaagtt gcgcgggcat atttagtata
     7741 tgtttttttt acaccggaag aagtagcaaa ctgaatggtg tctatattat tcaataacac
     7801 aacttctgcc gataccctgt atttttcatc gggctgataa aaccttaaca atgttgcttg
     7861 ttcttttgta acgggttttc gaggctcact caacaattcg tcaatatatt ttttacgata
     7921 gtcgtttatc gaatctgcat aactttgttg gccacataaa tcaaacgaag taataaatgt
     7981 gagtaaataa aaaataagta accctgtgct tttcattttc agataaattt tagctaaatt
     8041 attaatacaa agaaatagaa aataccatca tcacataaat taaagtatat ttacaaagcg
     8101 aacatttttt gctgtttaat tttgcaataa ctaaaatgga ttctttttca ccaatttcga
     8161 ttattttatt taataatgat ggtagtgttg cagctgctaa ctacaatgta tctataattt
     8221 ttaaagataa ggagaataat caattacaaa atattaagca actggaaaat aacattgccg
     8281 gcattacaaa tttttttaat gattatacaa aaaccacaaa agacaaacca aaccacattt
     8341 caaatatttt tacatttagt gatgcttcaa aattaaacca ttatcagtta acgttttcag
     8401 cgttgaaaac acaaagtgat aataagttct tagcattgat atacaaaata aataatgatg
     8461 acattgctaa tatgtcgaca tggaatgaag agcaatttca ggcatttatt aatagtgccg
     8521 gacattacat ctatacgctc gatcttaact tatgcataac atccttcaac aagtcgttta
     8581 aaaaagtttt ttttgatatt ttcaaacacg aaataaaaat cggagaaaat cctgacgatt
     8641 ttgtgccacc caaaatttca aattcgtgga gcgatcatta cttaaaagtt attagtggag
     8701 agcaggtttc atttccatat gcaataggca atggatattt ttttgttgaa atgtttccta
     8761 taaaaattga tggcaaaatt agcggcataa gcgtttttag cgaagataaa actatcgaac
     8821 aaacattgat aaatagcctc aaagaatcga agtttattca tgagtttgca atggatgtaa
     8881 gcgattccgg attttgggac tggaacctgg agaccaatga agttgtttac tccgacatat
     8941 ggaaaagcat gttaggatat gcacctcacg aaattaaaaa tacttatttc tcctgggaat
     9001 tacttgtgca tcccgatgat ttgccaaatg ccttgacaga ggttaagaaa cacattaacg
     9061 gtgaaacaaa aacttattcg gtgcaaaaaa gaatgttgtg caaagatggt agctacaaat
     9121 ggatacttgc taaaggaaga attgtaactt acgatgaaaa aggaaaaccg aaacgtttta
     9181 ttggcgtgca cattgatgtt gacgaagtat ataataccag gcaggaactg aaaaacaaaa
     9241 atcttcaatt agaaaaaata gcttttataa atgcacacaa aattcgtaag ccactggcaa
     9301 atattcaggg gcttaccagt ataatgaagg ataaacttgt tgacgaaaac gaactgaaca
     9361 attatctcac aatgcttctg gagtcgatta acgaattgaa tgagcaaacc aatgcattga
     9421 acaatacaat aaataaagaa gaaattaaag cattggctga ctcaagaaag gcattgaatt
     9481 ctatacggaa tattttggtg cttgatgatg accctattaa caattatatt gccataaaaa
     9541 tgattcaaaa gacaggtgtg agttgtgttt cgtttacaaa gccgagcgaa gcatttgaat
     9601 atctgaaagc gagcaatgat gtagatttaa ttttacttga ccttaacatg cccgtttata
     9661 acggatggga atttctcgat aatcttgaaa aagcacaaat aaatactccg gtaattatag
     9721 tgtcatcgtc atcgaatcag aaagataaat tgcgtgccaa aacgtataaa aatgtttgcg
     9781 attattggat taaacctctt aaggtggaac tactcaaaaa tttaatatcc tgatttattt
     9841 gtttaagaat atgtcgcagt caattcaaaa gaaactccgt atacatgaag ggttagacat
     9901 tacactttta aacgcgccaa caacttattt caaaacagta ggagaattgc cttcgggcgt
     9961 tactacaagc ttttcgctta agaaaaaaac cggttatgta catttgtttg tagccaacaa
    10021 atcggaactg gaaaaatact ttccgattat ttgtaaatta cttaagcctg aaggtttgct
    10081 atggatatcg tttttaaaaa agaaaaaaaa tgccagtaca gatttaacac gcgatacggg
    10141 ttgggaatgc ttaacacaac ttcccatgaa atggataacc cttatttcgt ttgatgataa
    10201 ctggagtgcg tttctgatgc gcaatgtacc tcccgaaaaa atgaccttaa acgaaatttt
    10261 gcacaatcat gaaacaaaca aaagcgaaaa aaaactacaa ccaaaagagc taattctacc
    10321 aatggatttg cagcaattat tattaaaaaa taaatcggct aaatcgtttt tcgattcctt
    10381 gagttatacc aatcgtaagg aatatgtgat gtgggttgac tcggcaaagc gtgatgaaac
    10441 caaaagcgat aggctgcgca aaacaatcga aaaactcaat gccggtttga aaaacccttc
    10501 ggcaaagtga ttgcactatt ttaattaaac catcctgcgg tgatagttta attgcccaag
    10561 atgataattt aaatgtgagt aaagatgaag tagcaattgc aatgtggtcc attccttttc
    10621 ccatttattt ataggataaa ttttcgccag gtcatcatct ttcaaattat cgaaagtgtt
    10681 tttcaccata acaatggttt ggttcaactg cataacaata agttgacggg caatattttt
    10741 tgaattaaac tccgcatcgc gctcccttat ataacctgtg ttgccaagcg ttgcaccaat
    10801 aaagtgattg agattgccca caatgtgcag gcacaaattg ccgccactat ttaaaacgtc
    10861 accacgcaat tcccacattt gcttttcgtt ttggtagctg tttatttctg caatcatttt
    10921 attcaagtcc cgctcgaata attcttttaa taattgaact gtcatttgat tttaaattaa
    10981 tttaatatct ctattgcgaa attttatttc aaaacttatg tggctgttgt aaatgcgaag
    11041 ttagaaaacc ttttcacaca aaagaatgtg aatatgtttt gtttcgatac tttatttatc
    11101 aatgataaaa aatatacttt tcaccatatt taattcaagg taaaattatc agcagatggc
    11161 acttagcagg gtttggtcct atattatttt attctccatt attgtagcgt gctatcacta
    11221 tttcaatggc gataaaaata tttttaatga tttggttaca gaaaaaaaat taaaagaaaa
    11281 tgaaagtttt tccaatcaaa atatggccat tcctttttcg ctatcggcca ccaacgatag
    11341 catagtgaat aagctgacat ctaaaatgaa ggccgcgaat tttaatatca ccgaagattt
    11401 actaaaagca aactacattg taactgacga taatgatgat gataaatggc gtaatttatt
    11461 gaagcagaaa ttggaagccg ttaccattat taattttaaa aatattaaca acaatctaaa
    11521 aaccgaaacg gattcggctg cggcaattta tgttcacacg ctgaatgatg cagacaaaac
    11581 tttgctcgat acactaataa gcaaatcgca tttgcacacc actgaaaata ttcttgcagc
    11641 aaaatatgtt gttaccagtt caacaaatac cgattcgctt agtaaaacat ttttattgcg
    11701 gactgaaaga ataaagatgg taagcgcaga aaaattgttt tactacttac ctaaaaacat
    11761 tgatggcatt ttcgaaacag gcaaatttgc catcaccctt gtgctgggct taattggcac
    11821 acttgcactt tttatgggat tgctaagcat tgccgaacat gcaggcggca tcaatgttat
    11881 cagtagattc atgagtccat tcttatcgaa attatttccc ggagtgccaa aaaatcatcc
    11941 ttccattgga cacatggtta tgaatttttc ggccaatatt cttggccttg ataatgcagc
    12001 aactcctttt ggtttaaaag ccatgcagag tttgcaagac attaatccaa acaaagacaa
    12061 agccaccgat gcacaaatta tgtttcttgc tttgcatgct tcgggcttaa cattaatacc
    12121 ggtttcaatt attgctgtgc gtgcggccaa tggttctatg agtcccaacg aaatttttat
    12181 tccactcata ctttccactt ttgtgtgttc gttaacggcc atgttaataa cctctttcaa
    12241 acaacgcatc aacattcttc aaccattaat tttctttccg ctgtttatca ttacttcgct
    12301 gttaaccgtt ttcgtttggt atttaaaatc gttgccggcc tttgccttag aacaattttc
    12361 cgactcgttt agcaacggtg ccattctctt tgttttttta ataatagttg caggaggcat
    12421 gtataaacgc ataaatattt tcgatgcatt tgtagaaggt gccaaaggtg gattcgaaac
    12481 tgcggtgcga attattccat acatggtggg catgctggtt gcagtaagtt tgttgcgtac
    12541 cagtggtgca ttcgatttgg ttattaatcc cataaaaaca tttatagcat ttaccggcat
    12601 ggatacacaa tgggcagaag gtttgcccac cgcgcttttg cggccattca gtgcaggtgg
    12661 tgctcgcgga tttatgattg atgccatgcg cacttacggc cccgattcgt ttattggcaa
    12721 aatggtttgt gtgttgcaag gctcaagcga aactactttc tatgtagtgg cgctttactt
    12781 tggttcggta gcaataaaag atacacgata ttcaattccg gttatgttgc ttgccgactt
    12841 agcaggaatt attttttcgg tttgtatttg ttattatctt tggagttaaa attttcagac
    12901 tcaaaaaatc attacactat tttcattttt gctttttgct ggcgcacact gatgaaaata
    12961 aatattgtac ccaatgctgt tggcaccaac caattcatta ttgcgattgg aaatatgttt
    13021 tgtattactg taaaagctgt agttgccatt atataagaaa tatacattcg tttaaaatgt
    13081 tcgaagtacc agttcatttt atggttatac atttttttta ccggcctcag caaaacatat
    13141 tgcataacat cgggtataga agttgcaaaa aatataaaag taaaaacaga aaatattatg
    13201 cccgaattta tgtcaccact cataaaagtt tcaatggcaa aatacaacat gtacaaattt
    13261 aaaagtgcac ctgctattac cacacctttt tgaaccgaag tttttaaatt gctttttcca
    13321 tgggcaaata aaaatcctgt gaggcaaaag taataaccaa atgcgccaat aaaaccaatc
    13381 ataaccgaac caattacaat tcctgaagaa atcacaacag tcatacatag cacgtaagtc
    13441 acccccatca ttcggtgata tttacctcct tttttcaaaa ctatttgcaa aaatcccaaa
    13501 accaaaacca aagtaccaac aatggcgtgg cctgtacgta aaaattcatc catatttatt
    13561 ttgctttaac tcatattaaa cacgtgcaat actatgttat ttttatattt gtcgccacaa
    13621 aaatcaagag tactatgtca aaacattttc cgaaattcat tttatcactg gttttactta
    13681 tcaatatttt ttctgccaag gcacaaattg tagatccgat aaaatggagt ttcaaagtag
    13741 aacaaaaaaa tgccgatgaa gctacgttat tattcactgc caaaatagac gaacactggc
    13801 atgtgttttc acaatttcac actggtgatt ttggtttgcc cacggtgttt gatattaaag
    13861 aaaacaaaaa attttacaag cttattggca aagtaaccga gccaacaccc gatgaggaat
    13921 ttgaacccat aacaaaaacc actctcaagt actatcaaaa aagtgtaacg ttttcacaaa
    13981 aaatacaaat catttgtaag gcgccattca aagtgaaagg catgttaaat tatcaggcat
    14041 gtaatgatga aagttgcctt gcaccaaccg atgttgaaat tgtttgccag gtaaataaag
    14101 attcgaaaga agaaacatgc gatgttaaaa gtgtaaatga cgacacaaca aataccggtt
    14161 cgatattgat tgaagaaaaa gatactacta caaacgttgc agttgttgca ccgccaacaa
    14221 aattacctcc atcgagcgat ccatcaattt tagaaaaaga atgttcggaa aatgtagatg
    14281 aaaattattc tgccggatct ttgttgggca ctttcatttt atcattttta ggtggactgc
    14341 ttgcattgct tacaccatgc gtgtttccta tgataccaat gacagtaagt ttttttacca
    14401 agcgcagccc tacgcgcgca aaaggaatta gtaacgcaat tatttatgca tcttctatta
    14461 ttgccattta tgtttcattg ggtttacttg ttacattaat tgccggccca tcggcattga
    14521 acgaaatggc gagcaactct tttttcaact tactcttctt cgttatattt ataatttttg
    14581 cattgtcgtt ttttggcgca ttcgaaattg tattgccctc atcgtgggtc aacaaagccg
    14641 attcgcaaag tgataaagga ggtctgctgg ggattttttt tatggctttc acattatcac
    14701 ttgtttcatt ttcttgcacc ggccctatca taggaacatt gcttgttgaa gccgctcaaa
    14761 gcggttcggt gattggtcct gctgttggca tgtttggttt ttcgcttgca cttgctttgc
    14821 cctttgcttt gtttgccatg tttccaagtt ggttaaatac tttaccaaag tcgggaggct
    14881 ggcttaactc ggtaaaagtt tgtttaggtt ttattgaact tgcattggcc atgaagtttt
    14941 tatctaatgt tgatttggca tatcactggg gaatattaag acgcgaaaca tttcttgcta
    15001 tttggattgt catttttggt ttgatgggat tgtacctcat aggcaagtta aaattttcgc
    15061 acgatagcga cttaccattt atttccactc cgcgtttggt attcgccatt ctcactttta
    15121 cttttactat gtatttaata cccggtttat ggggcgcacc tttgcgcatc atcagcggtt
    15181 ttcctccacc cgacttttac aaagaatgga ttaccggtac tcatgaatgt cctcacaatt
    15241 taaattgctt tcacgattac gatgaaggaa tggcttatgc caaagctcaa aacaaaccgg
    15301 tgatgataga ctttaccgga tgggcatgtg taaactgccg taaaatggaa gacaacgttt
    15361 gggtagaccc aaaaatattg aagcgtattt cagaaaatta tgttttgata tcgttgtatg
    15421 ttgacgataa aaagacgtta gatggtggtg ttcaaaaatc tccttacagc gaaaaacaag
    15481 taaaaacaac cggacaaaaa tggagcgatt tacaaaccgt aaaatactta tcgaatacgc
    15541 aaccatacta tgttctgctc gatcataacg aacgtattct tgcaaagcct cgtggctaca
    15601 cacctgatgt agaaacttat tcaaaattcc ttgacgaagg tgtttgccgt ttcgaaaaaa
    15661 gaaagagtgc ttctttatag aaaaaaatat tttgccgctt agagaaaaat attttaccgc
    15721 aaagagaaaa atatttcacc gcaaagagcg caaagaagga atcgcaaaga atacaaagta
    15781 ttagtttgcg atagttaaaa gaaacattgc gtcatttgca atataaaaaa agcattctaa
    15841 ttctttgcgc cttttgcggt aaaaaaacca tcctatttct ttgcgccttt tgcggttttc
    15901 ttttgcgctc tttgcggtaa aaaaacatct ctattcttct ttgcggtaaa aaaaccatcc
    15961 taattctttg cgccttttgc ggttttcttt tgcgctcttt gcggtaaaaa agcattctaa
    16021 ttctttgcgc cttttgcggt attcttttgc gctctttgcg gtaaaaaccc caaacaatat
    16081 ttgatgcaaa atactcttac cttgtatata ggaccacaca caattttctt caattcattt
    16141 tccaaacaaa ctgcataacg aaatttcgcc ctgcttgcgg gaaataaaaa ttctccgtaa
    16201 ttctttcatt accataaata taaccataag tatatccgtt gctgacatat cttttatcaa
    16261 gcaaattatt tatcctaaaa ttcaattgta taactccgat gctcttaaac tctttttcaa
    16321 aaatcaccga agcaccattg actaaatatt gtggaagcat tttcgcttcg ttttgggtgt
    16381 tatccaaatg ttgcttgcct acatatttta tttcatagtt aagtttcaaa aaacttgtta
    16441 cagaatacat acaggataca aaacctgtag tagcaggcga aaacgaaaca tctac
//
LOCUS       DAORQA010000032        14578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1103_length_14578_cov_24.434759, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000032 DAORQA010000000
VERSION     DAORQA010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14578
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1103_length_14578_cov_24.434759"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..2326
                     /locus_tag="PK736_03910"
     CDS             194..2326
                     /locus_tag="PK736_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32569.1"
                     /translation="MTRIQIYIAMLCMMAIGANAQTTINGTAGTLNGPGGTIKDIKAA
                     EQSPANIRYKGETKIVDQFFAKEIDDDDEQSIERLMNEMQFTKDNSMRKSAPFYQIDE
                     NKQVSAPPTFEPGQKVTSQSPARTGLGNQQGGLDPSDAQIAAGPSHIVLLANDDARII
                     NKQSGAVISDVTLAAFFGMTGFVFDPRIMYDPYGQRFVALALNGATQTCGAGNVNSFF
                     RLLISYNSDPTQGWYWYDIDVDAANTAWMDFAMIGFNKDWICVAGNILCGSAPGIYVL
                     NKAQVYAAASANFYYWNSLWLAAPAFTYDNAIDRLYLVKTGNPNLNNTGYIDSWYINS
                     VPQLVQASSYGTSPWQAFATDSLKCPKQLGGGTTFSAVNIFGASMLSNCVYRNGLLWC
                     THPIFLPATGNTNRASTQWWSVNPAAPSISQVGRIDDGTGATSEYYPSLAVNSSSDVV
                     LTYSIFNAAYYQSAAYNSRNSADALGTLPNGVFYYSGQSYDSDGRGGDYSQCAVDPLD
                     QTSMWVVNQIPNGNWETRYSLLPGYYGCYTDASFGNNVWSGYTKNEASSSITSAEMIQ
                     SPSVVEYDAGGYVALTPGFVANQGSQFTAYINGCGGARTMSDKNNIEAAVPQQKQKVE
                     SKEIGVFPNPASNKCYITLGDMMDEKVNIKLYDMNLRHVKDLATDLNIVTKQTILEYD
                     IAAMQPGAYFIKIESSQVNSLQKLIIVK"
     gene            complement(2520..3278)
                     /locus_tag="PK736_03915"
     CDS             complement(2520..3278)
                     /locus_tag="PK736_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32570.1"
                     /translation="MIKKIIFFFAISAFISCGPPKEIHVNPVVVFELNDTMHYHIQAK
                     LIDIQGCYQINKKHAISIQPFLAGLGLGYFYTMRDAGKRNMLLLSANAGFLANEEGGS
                     MGSPYRGYKKFWDNKTKYQFISGTISYGFDDEHYGIKLGARYSLYNYLLFYRKLYHED
                     YISFSTSVPIYKDITTAKNLYGQCLSPYVHIFVRYDHICLFGQASYNFFNMRHVYYHE
                     VIPRGGPKDPDTGFKTRQATYNKTNINIGVSFDF"
     gene            3677..5113
                     /locus_tag="PK736_03920"
     CDS             3677..5113
                     /locus_tag="PK736_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32571.1"
                     /translation="MKKLFLSLAFIASAFMLNAQTIFNGGIYTNTTLTLANSPYLMTG
                     SVVVFPGVTLTIEPGVEVRVEKFSNNATVPYTLEVRGALYMVGQPGLPITFVGDTTGT
                     AQDTWNGIYIKNGQGGQLLGFDYVNISNAYQSVGYESTTTDVFDLHDCNFKYNGTGIN
                     VTTSVTADNCTFIGNGAGISGWSNFTLTNCILDSNNSALSIYPTAVSMKNCQLNYNNI
                     GLGFNASVFSSMLLDSSTFTHNNLAVYAPNGGSITNCDFNNNVWALGYTYDLLVNNCS
                     IQFNELGIDAGSDVIVQNSDISNNLVGVGLGPITFGMVMPLVQNNYICNNTQYNVENK
                     TDLNLTLASNCFCESDSTVIEEKIYDGYDDITRGLISYAIYDSSCSTILQYVNKFPTS
                     VEEIYASQISLQPNPATNYCSIVTSNLAGQNGFINISDASGKTIYSQFINADRIDIAT
                     DSFVAGVYHVSISADNNFITHKKLVVLK"
     gene            5483..7360
                     /locus_tag="PK736_03925"
     CDS             5483..7360
                     /locus_tag="PK736_03925"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRC32572.1"
                     /translation="MKPNYLSFSFALVLAIVCFSSFKINAQDGNNSGRHCGTQPMPVE
                     YENWLNQCRLQMIANGTAKMNTAYTIPVIVHVVHNGNAIGVGDNISDAQIYSQFDVLN
                     EDFKKLNSNWNQTPAVWTNLVADCEITFCPAKNDPNGNPLPTPGIERINRNNMGWPSP
                     PYDQTDCNTIIKPQSIWNPQNYLNVWSIDLGNGLLGYATFPPGTPLNGIPGNPGTMTD
                     DGVVILYNAFGRTGNLINNYDLGRTVTHEIGHWLGLRHIWGDGNCADDYCNDTPPATA
                     ANSGCPVFPHNVNSCGTNSDGEMFVNYMDYCYDYCLTMFSNDQKSRMQIAMANSPLRT
                     GFANSPAGCAGGGGLGGCDTITNFVIGVNTPQIYPMPNGTGYISGHNSFLDVSKADKF
                     LAAQYQPGSFVTGAVLYFGAASATNNTNTFDVKVWDDNGLAGSPGSPMGSEPITYLTA
                     STHVLQNALSLINFATPIAMSGDFYLGINFDYNVVGDEVALYTSTDGQTTPATAWEQW
                     DNLDWYRYDDSQSWAMNVCHIVLPVMCSPAGEEEVKTNGTLFLQPNPAQYKIKIMYDG
                     LNSNSEIKIFDVQGRLQQCPAENVMNGIEEFDISKLCPGIYFIRVADGEKILSKKFVK
                     Y"
     gene            7844..8959
                     /gene="gmd"
                     /locus_tag="PK736_03930"
     CDS             7844..8959
                     /gene="gmd"
                     /locus_tag="PK736_03930"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRC32573.1"
                     /translation="MKVALITGITGQDGAYLAELLLDKGYMVHGIKRRSSLFNTERID
                     HLYEDIHQKNVRFKLHYGDLTDSTNIIRIVQEVQPDEIYNLGAMSHVKVSFEEPEYSA
                     NADGIGALRVLEAVRILNLTAKCRIYQASTSELYGLVQEIPQHETTPFYPRSPYAVAK
                     LYAYWIMKNYREAYNMYTVNGILFNHESPLRGETFVTRKITRAVARIALGLDTKVYMG
                     NIDAQRDWGHAKDYVEAMWRMLQQPTPDDYVVATGVMTSVRDFIRMAFAQAGIEIQFE
                     GKDENEIAKVVKCNDARYQVAAGQVVLEIDKKYYRPTEVELLIGDPTKAKEKLGWVPS
                     HTLQQIVNEMVDADLALFSKDKYLKEGGHSVINYHET"
     gene            9533..10459
                     /locus_tag="PK736_03935"
     CDS             9533..10459
                     /locus_tag="PK736_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRC32574.1"
                     /translation="MITPPYLKAGDKIGIIAPARKVTPGSIDAGLQLLRNAGFEIVEA
                     KNLYGDNNQFSGSDNDRAHDVQDMIDRNDIKAIIAARGGYGCSRIVDEVDFAPMINNP
                     KWMIGFSDLTVFHSHIHQNLGVETIHANVLTCYNDAFDPQSLDTLIAALTGKTLQYEW
                     TTSEKNIAFNKHGEVTAEIIGGNLSLLHNLAGTESDMDVAGKILFIEDLDEYLYHIDR
                     MMVQLKRCNKLELLAGLVVGGMTNMRDNEIPFGKTAEEIIHEHTNAYGYPVVFDFPAG
                     HTPLNSALILGREATLVVGDSNQLIFHEGDDS"
     gene            10504..12216
                     /locus_tag="PK736_03940"
     CDS             10504..12216
                     /locus_tag="PK736_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRC32575.1"
                     /translation="MPKYFLLFTFISISSVTFCQSADEKYHQNETVTVKECIDFYKGL
                     SYKYANVHISQMGFTDMNQPLYCITVSKENIFDSTKTSILINNGIHPGEPCGVDACMV
                     WIKNILSSSDSKKLLDNVNLVIIPIYNVDGALNRNSYSRANQNGPKEYGFRGNAINLD
                     LNRDFMKCDSRNTFFFHRVFQLFKPEIFVDTHISDGADYTYTMTLIATQHNKFHPVLA
                     AYMDTVMLPFLYNGMDKKNNTMFPYVESVAEIPDSGIAGFLDIPRFSTGYTSLFNTIS
                     FVTETHMLKPYPAQVQATIDFLQTLLEFTHNNAAQIKAAKLKADKLTATQKSFDINWK
                     QTDTLTDSLLYKGYAAKYKKSNITGKDRLYYDEQQTITKKIPFYNECTATEKVIAPRA
                     YIIPQQWWRVIDILRNNKIEMSALQNDSMIEVNCYYITDYETTKKPYENHYLHSKIKT
                     RGEVQKIQFHKGDLIIYCNQQANNYLANLLEPAAVDGVFAWNHFDAILQQKEWFSDYV
                     FEDVAEKLLQTDAALKTKFDAYVKENKIENDANAQLIFLYRNSPYYEKSHNRFPVYKI
                     VISY"
     gene            complement(12333..>14578)
                     /locus_tag="PK736_03945"
     CDS             complement(12333..>14578)
                     /locus_tag="PK736_03945"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32576.1"
                     /translation="YVLGGWSYSNISGDKTENSNGGWDYWVVKIDSLGAIVWQNTIGG
                     NSSDGLYSITSTPDGGYVLGGDSNSNISGDKTENSNGIHDYWVVKLSPSSRITGKVYA
                     DNNHNNVQDSGEVNISGIKINEANNGRYTFSQSDGFYSIGVFDTGSFVVSPSQISYYI
                     PNPNSHTVNFTAMQQIDSLNDFAYQPTTVANDLYISIHPLSPFRSGFTATYNIHYKNI
                     GTTVLSPTIYFFNDAKVDFVSSNVLPDNIYADSVIWSLPALAPYGEGDITVTVYVHQG
                     LQIGTIINSSAKIFPFVNDYDIANNSASWEVTTIGSFDPNEIIVSTDSVSIFDLKPVP
                     PYLEYVIYFQNTGNDTAFNVKVENIIPYRLNLAAFEYVSSSVPVNMSINKESRLATFE
                     FPNIQLPDSNVDEPASHGYIRYRIKPQKTLNVGDTIFNRASIYFDFNSALLTNYATTE
                     IYFPGFNNAINGANSLCLDTNASWSYTVKQNADHTFFWNIIGGQIVSGQGTDSVNVKW
                     LVAGNHQIEVLECDTVSQFCDSNSFLITVNSLYNLVTSASICTGDSIYLANNWQTQSG
                     IYNDTLQSTQGCDSVVQTSLTINPTQTYTDTVNIVQGDSTFAGGMWQTQSGIYYDSLT
                     TQFGCDSLVIIVLNVFTSNNAITTTNNILTVRPNPAQNILEVIVNLAKFVGTQTLATQ
                     TFGTQTLALENITFQLIDIHGKVKLTATLTPNQKQIIDISSLASGIYVAQIIYEGRKY
                     EKKVLKE"
BASE COUNT         4721 a         2761 c         2832 g         4264 t
ORIGIN      
        1 gaagaaggtg gtatggctgt tggcgaaatg caaggaaaaa ttattgaaaa agtagaagaa
       61 caggcactgt acattataca attgaacaac aaaataaaag agcttgaaaa gaaaatagaa
      121 gcaatacagc ttaaataatc ccatatcatt ttattgaaaa atagtaagta acaaaaaatt
      181 aaaaataaaa gatatgacac gaatacaaat atatatagcc atgttatgca tgatggctat
      241 tggtgcaaat gcacaaacaa caatcaatgg aaccgcagga accttgaacg gcccgggagg
      301 gacaataaaa gatataaagg ctgctgagca atcgcctgca aacatcaggt acaagggcga
      361 aacaaaaatt gttgatcagt tttttgccaa agaaattgat gatgatgacg aacaaagtat
      421 cgaaagattg atgaatgaaa tgcagtttac aaaagataat tctatgcgta agagtgcacc
      481 tttttatcaa atagatgaga ataaacaagt gagtgcacca ccgacttttg aaccgggaca
      541 aaaggtaaca agtcaatcac cggcacgcac gggtttgggt aatcaacagg gaggattaga
      601 tccatcggat gcacaaattg ccgcagggcc ttcgcacatt gtattacttg ctaacgatga
      661 tgcacgtata ataaataaac aaagcggagc agtaataagt gatgttacac tagctgcttt
      721 ttttggaatg acaggctttg tgtttgatcc ccgcattatg tacgatcctt atggacagcg
      781 gtttgtagct cttgcgctaa atggcgctac tcaaacctgc ggtgccggaa atgtaaattc
      841 tttttttcgt ttgttgattt cttacaattc cgatcctacg caaggatggt attggtacga
      901 tattgatgtg gatgctgcaa ataccgcatg gatggatttt gccatgatag gttttaataa
      961 agattggatt tgcgttgcag gaaatatttt atgtggaagc gctccaggta tttatgtgtt
     1021 gaacaaagca caggtatatg cagccgcctc tgcaaatttt tattattgga attcattatg
     1081 gcttgccgca ccggcattta cttatgataa cgctatcgac agattatatc ttgtaaaaac
     1141 aggcaatccc aatttgaaca atactggtta catagactca tggtacataa atagcgtgcc
     1201 acaacttgtg caggcaagca gttatggaac cagcccatgg caggcgtttg caaccgatag
     1261 tttgaaatgt ccaaagcaat tgggaggagg aacaaccttt agtgctgtaa atatttttgg
     1321 tgcatctatg cttagcaact gtgtatatag aaatggttta ttatggtgca cacatcccat
     1381 atttttgcct gctacaggca ataccaatcg agcctcaaca cagtggtggt ctgttaatcc
     1441 cgcagcgcct tccatcagtc aggttggtcg tatcgatgat ggtacaggag ctacttccga
     1501 gtattaccca agccttgctg taaactcctc atcggatgtt gtgcttactt atagtatttt
     1561 taatgcagct tattatcaga gtgctgcata caattcgcgc aatagtgccg atgcgttggg
     1621 aacactgccc aatggtgtgt tttactacag tggacaaagt tatgattctg atggtcgtgg
     1681 tggtgattat agccagtgtg ctgtcgatcc gcttgatcaa acttcgatgt gggtagtgaa
     1741 tcaaattcct aatggtaact gggaaacaag atattcgtta ttgcctggat actatggatg
     1801 ttataccgat gcctcttttg gaaataatgt atggagtgga tatacgaaga acgaagcatc
     1861 gagttcaatc acctctgccg aaatgattca atcaccatcg gtagtggagt atgatgcagg
     1921 tggctatgtt gcgcttacac cgggctttgt tgccaatcaa ggttcgcaat tcactgccta
     1981 cataaatggt tgtggtggcg cccgcactat gagcgataaa aacaatattg aagctgcagt
     2041 accacagcaa aaacaaaaag tggagagtaa agaaattggc gtatttccaa atcctgcttc
     2101 caacaaatgc tacatcacac tgggcgatat gatggacgaa aaagtaaaca taaaattata
     2161 tgatatgaat ctacgacatg taaaagacct tgccaccgat ttgaatattg taacaaaaca
     2221 aacaatactc gaatatgata ttgccgcaat gcaaccggga gcatatttta taaaaataga
     2281 atcatcgcag gtaaacagtt tgcaaaaatt gatcattgta aaataaaatt ataagcatca
     2341 aaaaacaaaa gcgacataca gtaatgtgtg tcgctttttt tattctataa acaatatttt
     2401 agaaaggaaa aaaataagat ggggttttat attttgcact tctattaatg caaagttgta
     2461 aaataaaagt tgccaaactt tgattttgtt tcaaaagaaa tttacagcaa aaagaacatt
     2521 taaaaatcga aacttactcc gatatttata ttggttttgt tgtaggttgc ctgccgtgtt
     2581 ttgaaacccg tgtccggatc ttttggacca cctctgggta taacttcgtg atagtaaaca
     2641 tgcctcatat tgaaaaagtt atagctcgct tgaccaaaca aacaaatatg atcataccgc
     2701 acaaagatgt gaacataagg agaaaggcat tgcccgtata aattttttgc agttgtaata
     2761 tccttatata tgggtaccga tgtgctaaaa ctaatataat cctcgtgata taattttcta
     2821 taaaacaata ggtaattgta aagagaataa cgtgcaccga gtttaatacc ataatgttca
     2881 tcatcgaagc cataacttat tgtgcccgaa atgaattggt acttggtttt attatcccag
     2941 aattttttat acccacgata gggcgagccc atactgccac cttcttcatt agccaaaaag
     3001 cctgcattgg ccgacaacag cagcatgttt cgtttgcctg catcgcgcat ggtataaaaa
     3061 tatcccaggc ctaaaccggc aagaaatggc tgaatgctta tggcatgttt tttatttatt
     3121 tggtagcaac cttgtatatc tataagtttt gcttgtatgt ggtaatgcat ggtgtcgttc
     3181 aattcaaaaa ccactaccgg atttacatga atttcttttg ggggcccaca cgaaatgaaa
     3241 gcactaatgg cgaaaaagaa aattattttt tttatcatgt tgattgaata ttttgtgttt
     3301 caaatatact tatttccaaa ttataaaagg ttggtcacaa aatattagca tctatattca
     3361 ttatcttaaa aacgaaaagg aattcgaaaa ataaaatgaa agaaagcccc aaaaaaccaa
     3421 taggataaat aaccacctat cgaaaaaaaa caattccaat tctaatcaag ttacgtcaaa
     3481 caaaatttgt tcagctagtt ttattgggta ctaccatttc tttatcagta tcccttctct
     3541 gcttaatgtg ttgaaccctt tttttggaaa aactttctat cataaaacat aaaattatct
     3601 agtgttaaaa aatattacac ttaaaaactt tatactgtta ttcgggttta aaatctacat
     3661 ttaaaacaaa acatacatga aaaaattatt tttatctctt gcattcattg catcggcatt
     3721 catgcttaat gcccaaacta tttttaacgg tggtatttac acaaatacca ccctgacact
     3781 tgccaatagc ccgtacctta tgacaggttc ggtggtggta tttcccggtg ttactttaac
     3841 cattgaaccc ggtgtagagg tacgggtaga aaagttctcc aacaatgcaa cagtgcccta
     3901 tacacttgaa gtgcgtggtg ctttgtatat ggtggggcag ccgggtttac ctatcacatt
     3961 tgtgggcgat actaccggta cagcgcaaga tacatggaac ggcatttaca ttaaaaatgg
     4021 acaaggaggg cagcttcttg gttttgatta tgtaaatata agtaacgctt accaatcggt
     4081 aggctacgaa agcactacca ccgatgtgtt cgatttgcat gattgtaatt ttaaatacaa
     4141 tggcactggc ataaatgtta ccacttctgt tacagccgat aattgcactt ttattggcaa
     4201 cggagccgga atctctggat ggtctaattt tactttaact aattgtattc tcgatagcaa
     4261 caacagcgcg ctttcaattt accctactgc tgttagtatg aaaaactgtc agctcaatta
     4321 taacaatata ggtctgggtt ttaatgccag tgtttttagc tccatgcttc tcgatagcag
     4381 cacattcaca cacaacaact tggctgtgta tgctcccaac ggtggcagca taaccaattg
     4441 cgacttcaac aataatgttt gggctttagg ttatacttat gatttgcttg taaataactg
     4501 tagcatacaa tttaacgaat tgggaataga tgcaggctcc gatgttattg tgcaaaacag
     4561 tgatatcagc aataaccttg taggggtagg attagggcct ataacttttg gaatggtaat
     4621 gccattggtg caaaacaatt atatctgtaa caatacacag tacaatgtcg aaaataaaac
     4681 cgatcttaat ttaacgctgg ccagtaattg tttttgcgag tcggattcta ctgttattga
     4741 agaaaagatt tatgatggct acgatgatat aacccgaggc ctaataagtt atgccattta
     4801 cgatagttca tgctcaacaa ttttacagta tgtaaataaa ttcccaacaa gtgtcgaaga
     4861 aatttatgct tcacaaattt cgcttcagcc aaatcctgca acaaattatt gttctattgt
     4921 aacaagtaat ttagccgggc aaaatggttt tataaatatt tctgatgcca gtggcaaaac
     4981 gatttattca caattcataa atgccgacag gatagatatt gctaccgata gttttgtggc
     5041 cggtgtatat catgtaagca tttctgccga taataacttc ataacgcata aaaagctggt
     5101 tgtactaaag taaaaattgt ttgtatttta ttgatagttc ttaagcctgt tcaatttatt
     5161 ttggacaggc ttttttttgc attttttttt gaaataattt gtggaactaa ttccagacta
     5221 actttttaat tcattacaac ccaacaaaag aaacaccctt tcgaaaaata ataacagaga
     5281 atatacagta gcaaaaaaag gttattacaa acttcacctg cagaaaacac acaaaccgcc
     5341 tgtttaaaac tttggccaaa atagaatgaa ttgttcaaac aaaatgtttg agcttatgat
     5401 attttattat atgtttgcgt aaccattatg tttttaagta aaaacattgc cacttactag
     5461 taacactaaa aaattcaaac aaatgaagcc aaattacctt tcattttctt ttgcccttgt
     5521 tttagcaatt gtatgctttt ccagtttcaa aattaatgct caagatggaa acaattcggg
     5581 caggcattgt ggcacgcaac caatgcctgt tgaatacgaa aactggctca atcaatgccg
     5641 tttgcaaatg atagccaatg gcactgcaaa aatgaacact gcgtatacta tccccgttat
     5701 tgtgcacgtg gtgcacaatg gaaatgctat tggtgtgggc gataatatta gcgatgcaca
     5761 gatttattcc caatttgatg tactcaacga agattttaaa aagctcaaca gcaactggaa
     5821 tcaaacaccg gcagtttgga ctaaccttgt ggccgattgc gaaataactt tttgccctgc
     5881 aaaaaatgac cccaacggaa accctctgcc cacaccggga atagagcgaa taaatcgcaa
     5941 taatatggga tggccctcac caccatacga ccaaaccgat tgcaacacta tcatcaagcc
     6001 acagtctatt tggaatccac agaactatct aaatgtttgg tccattgatc ttggaaatgg
     6061 gttacttggt tatgcaactt tccctcccgg aacaccactg aatggcattc ccggtaatcc
     6121 tggaacgatg accgatgatg gggttgttat tctttataat gcttttggaa gaacagggaa
     6181 tcttattaat aactatgacc tgggccgcac tgtaacacac gaaataggac attggttggg
     6241 gctgcgtcat atttggggcg atggcaattg tgccgatgat tattgcaacg atactcctcc
     6301 agctacagca gctaatagtg gttgccctgt atttccgcac aatgttaaca gttgtggaac
     6361 taatagtgat ggcgaaatgt ttgtgaatta catggattac tgttacgatt attgtttaac
     6421 aatgttcagc aacgatcaga aatcgcgtat gcagatagcc atggcaaaca gtccgctgcg
     6481 cacaggtttt gccaactctc ctgccggatg tgctggtggt ggtggacttg gcggttgcga
     6541 cactataaca aattttgtta ttggggttaa tactcctcaa atttatccta tgcccaatgg
     6601 tacgggatac atatcggggc acaatagttt tttagatgta tcgaaggccg ataagttttt
     6661 agccgcacaa tatcagcccg gttcatttgt taccggggca gtgttatatt ttggtgctgc
     6721 atcggcaact aataatacca acacctttga tgtaaaagtt tgggacgaca acggcttagc
     6781 cggttcaccc ggttcaccga tgggctctga gccaataaca tatcttacag catcaactca
     6841 cgttttacaa aatgcacttt cattaataaa ttttgcaacg cctattgcta tgagtggcga
     6901 tttttacctt ggaataaatt tcgactataa cgtagtaggc gatgaggtag cgctatacac
     6961 cagtaccgat ggacaaacaa cacctgccac tgcctgggaa caatgggaca atctggattg
     7021 gtacagatat gacgattcac aaagttgggc aatgaacgta tgtcatattg tgctgcctgt
     7081 tatgtgctcg cctgccggag aggaggaagt gaaaaccaat ggaactttat ttttgcagcc
     7141 taatccggca cagtataaaa taaaaataat gtatgatggc ctcaactcaa attcggaaat
     7201 aaaaatattt gatgtacaag gaaggctgca acaatgccct gcagaaaatg taatgaatgg
     7261 cattgaagaa tttgatattt ctaaactatg ccccggaatt tattttattc gtgttgccga
     7321 tggcgaaaaa attctttcca aaaaatttgt gaaatattga ttcatgattt acagttctca
     7381 catagtttta aatgtgagtt tgtgttttta atagggcaaa attccaattg gtatttgtgc
     7441 aaataagaat gcatactctc acactattgt tttaattaaa aaataacgca agcttatttc
     7501 aaaagaattc gctccgtgcg ttttcttgtt aaattttttt gcaaaagttg atgaaagaaa
     7561 tatggtggaa cgactactta aaaattactc ttaatacgat ttatttcgcg catttgtttg
     7621 atggagttat ttataaaggg taataaaaac tcttcaattt cgaatgctat ttttcatgta
     7681 aatatttttt gttcttaatt atatatggtg ttcacttgtt ccgtttttaa atagcaaagt
     7741 tcatcaacaa ccgcatgtgt atagaattga aaacacacca acccaattaa ttttttttta
     7801 catcaatatt cgccttcttt accaagaaaa taaaaatgca aaaatgaaag ttgctcttat
     7861 taccggcatt acaggacaag atggtgctta ccttgccgag ttgctgctcg acaaaggtta
     7921 catggtgcac ggcatcaaac gaaggagttc gttattcaac accgaacgaa tcgatcattt
     7981 gtatgaagat atacatcaaa aaaatgtgcg cttcaagtta cactatggcg accttaccga
     8041 cagcacaaat attattcgca tagtgcagga agtgcagccc gatgaaattt acaacctcgg
     8101 tgccatgtcg catgtaaagg taagtttcga agagcccgag tactctgcca atgccgatgg
     8161 tataggcgca ttgcgtgtgc tcgaagctgt gcgaattttg aaccttactg ccaagtgccg
     8221 catataccag gcttctacat cggagttgta tggtcttgtg caagaaatac ctcagcacga
     8281 aaccacaccg ttttatccgc gctcaccata tgctgttgca aaactatatg cctattggat
     8341 aatgaaaaat tatcgtgaag cgtacaacat gtacaccgtg aatggaatat tattcaacca
     8401 tgaatcacca ttgcgtggcg aaacatttgt aacacgaaaa attacccgtg ctgttgcgcg
     8461 catagcactc ggcctcgata caaaagtata catgggcaat attgatgcac aacgcgattg
     8521 gggacatgcc aaggattatg tggaggcaat gtggcgcatg ttgcagcaac ccacgcccga
     8581 tgattacgta gtggcaacag gtgtaatgac aagtgtgcgc gattttatac gaatggcatt
     8641 tgcacaggcc ggcatcgaaa tacaatttga aggcaaagac gaaaacgaaa ttgccaaagt
     8701 tgtaaaatgt aacgatgcac gctatcaggt tgctgccgga caagtggtat tggaaataga
     8761 taaaaaatat tatcgcccta ccgaagtaga attgctaata ggcgatccta caaaagcaaa
     8821 agaaaaactt ggttgggtgc catcgcatac attgcaacaa attgtaaacg aaatggtaga
     8881 tgccgattta gcattattta gtaaagataa atacctcaaa gaaggtggac atagcgtaat
     8941 taattatcac gaaacttaga ggatggttat tggttgattt gctattgggt tattgggttg
     9001 attggttgtt gactttgtca tgctgaggta cgaagcatcc ctgccggtca ttggtcattt
     9061 tcgtcattgc gatttgtcac agtgagtatt ctcgaactgc gaagcatctc tgccggtcat
     9121 tggtcattat cgtcattgca aaggaggagg agtgagtact ctcgaaacga ctgaagcaat
     9181 ccctctttag aaaagacttg acgatgatga gattgcttcg ctacgctcgc aaagacgtga
     9241 gaaaaaaact ccgaaaacaa aacaaagaac aaggcgtacc gaaaacgaaa caaaaaattt
     9301 cagagtcctc atttattttt tgtttttaat tggcatgaga gactctgaaa ttattaacgc
     9361 ttcgcaaaca aaactaagaa ctaagaacat ggcacaaagt aaacgtaact aagaacatgg
     9421 caccccgcaa acaaaacaaa gaacgaagaa catggcaccc ctaaaacgaa actaagaact
     9481 aagaacaaag aacaaggctc aaagcaaacg taactaagaa ctaagaaaaa aaatgataac
     9541 accaccctac ttaaaagcgg gcgacaaaat tggaataata gctccggcac gaaaggttac
     9601 accaggaagt atagatgctg gtttgcaact attgcgcaat gccggtttcg aaatagtaga
     9661 agcaaaaaac ctttatggcg ataacaatca gtttagcggc agcgataacg atcgtgcgca
     9721 tgatgtgcaa gacatgattg accgcaatga tattaaagca ataattgctg cacgcggagg
     9781 ttatggttgc tcgcgcattg tagacgaggt tgattttgcc cctatgatta ataatcctaa
     9841 atggatgatt gggtttagcg acctcactgt ttttcattcg cacatacatc aaaacttagg
     9901 agtagaaacc attcatgcaa atgtgctcac ctgttataat gatgcattcg atccgcaatc
     9961 gctcgataca ttgattgccg cactcacagg taaaacgcta cagtatgaat ggacaacaag
    10021 cgaaaaaaat attgccttca acaaacacgg agaagttact gccgaaatta ttggtggtaa
    10081 tttatcgttg ctgcacaacc ttgccggcac cgaaagtgat atggatgtag caggaaaaat
    10141 tctttttata gaagacctcg atgaatactt ataccacatc gaccgcatga tggtacaact
    10201 taaacgatgc aataaattag agttgcttgc cggacttgtt gttggtggca tgactaatat
    10261 gcgcgataac gaaattccat ttggtaaaac tgccgaagaa ataatacatg aacatacgaa
    10321 tgcatatggt tatcctgtag tgttcgattt tcctgcaggg catactcccc tcaatagcgc
    10381 actcattctt ggccgcgaag caacgctcgt tgttggcgat agcaatcaac ttatatttca
    10441 cgagggtgac gatagttagt gttttgtttt acatatttgt gtagtaattt ttaatactac
    10501 atcatgccta aatatttcct tctctttact ttcatttcta tttcgtcagt tacattttgc
    10561 caaagtgcag atgaaaaata tcaccaaaat gaaaccgtca ctgtaaaaga gtgtatcgat
    10621 ttttataaag ggctctctta caaatatgca aacgtgcaca tatcccaaat gggattcacc
    10681 gatatgaatc aacccttgta ttgcataact gttagtaagg aaaatatatt cgattccact
    10741 aaaacaagta tactcatcaa caatggcata caccccggtg aaccctgtgg tgtggatgca
    10801 tgcatggttt ggataaaaaa tattctgagc agcagcgatt caaaaaaatt actcgataat
    10861 gtaaaccttg ttatcattcc tatttacaat gtggatggtg ccctcaaccg taacagctat
    10921 tcgcgggcaa atcaaaacgg accaaaggaa tatggcttta ggggcaatgc aattaacctc
    10981 gacctgaacc gcgattttat gaagtgcgat tcgcgcaaca cttttttctt tcatcgggtg
    11041 tttcaattat ttaagcccga aatatttgtc gatacacaca ttagcgatgg tgccgattac
    11101 acttacacca tgacgcttat tgctacacaa cacaacaaat ttcatcctgt gcttgcagcg
    11161 tatatggaca cagttatgct gccatttcta tacaatggaa tggacaaaaa aaacaatacc
    11221 atgtttccgt atgtagaatc cgttgctgag attcccgatt ccggcattgc cggctttctc
    11281 gatataccaa ggttttcgac tgggtacaca tcgctcttca atacaatatc gtttgtaacc
    11341 gaaacacata tgctaaaacc ttacccggca caggtgcaag ccactatcga ttttttgcaa
    11401 acgctattgg agttcacgca caacaatgcc gcacaaataa aagcggcaaa actgaaagct
    11461 gataaattaa cagccacgca aaaaagtttc gatattaatt ggaaacaaac ggataccctt
    11521 accgacagcc tgctgtacaa aggctatgca gcaaagtata aaaaaagcaa tattacaggt
    11581 aaagacagat tgtattacga tgaacaacaa accatcacaa aaaaaattcc tttttataat
    11641 gaatgcactg ccaccgaaaa agtaatagca ccacgcgcat atattattcc acaacagtgg
    11701 tggcgggtga ttgatatttt acgaaataat aaaattgaaa tgtcggcatt gcaaaacgat
    11761 agcatgatag aagtaaactg ttattacatc accgattacg aaacaacaaa gaagccttac
    11821 gaaaatcatt atttgcacag caaaataaaa acccgcggtg aagtgcagaa aatacaattc
    11881 cataaaggtg atttgataat atactgcaat caacaagcga ataattattt agcaaattta
    11941 ctagagcccg ctgcagtaga tggtgttttt gcatggaatc atttcgatgc catactgcaa
    12001 caaaaggaat ggttcagcga ttatgttttc gaagacgttg ccgaaaaact tttgcaaacc
    12061 gatgccgcat taaaaacaaa attcgatgcc tatgtaaaag aaaataaaat tgaaaacgat
    12121 gccaatgcac aactaatttt tttataccgc aactcaccgt attacgaaaa gtcgcacaat
    12181 aggtttccag tgtataagat tgtcattagt tattgaggat tgtcattagt cattaacgac
    12241 atagcgtgaa gcaatctgaa cttgtcattt tttttccgtc attgcgattc cgattactat
    12301 cggaagaagc aatctgcact tatcattggt cattattctt tcaatacttt cttttcatac
    12361 ttgcgccctt cgtatataat ttgagctaca taaattccac ttgcaagaga cgaaatatca
    12421 ataatttgtt tttggttggg agtgagcgtt gctgtgagtt tcaccttgcc gtgtatgtca
    12481 atcaattgga aagtaatatt ctctaaggcc aaggtttgtg tcccgaatgt ttgtgtcgcc
    12541 aaggtttgtg tccccacaaa ctttgctaaa ttcacaatta cctccaatat attttgtgcc
    12601 ggatttggcc gcacagtcaa aatattattt gtggttgtga tggcattgtt ggatgtaaaa
    12661 acattgagca caattatcac caaactgtcg caaccaaatt gtgttgtgag cgaatcgtaa
    12721 tagatgccac tttgtgtttg ccacatgcct ccggcaaatg tgctatcacc ttgtacaatg
    12781 ttaactgtat cggtatacgt ttgggttggg ttaatggtga gcgaagtttg caccacagaa
    12841 tcgcaacctt gcgtgctttg taaagtatcg ttataaatgc ccgattgtgt ttgccagttg
    12901 ttggcgagat aaatgctatc gcctgtgcaa atgcttgcag atgttactaa attatacaaa
    12961 gaatttacag ttattaaaaa agaatttgag tcacagaact gtgaaactgt atcacattct
    13021 aaaacttcaa tttgatgatt accagccact aaccatttta catttacgct atctgtccct
    13081 tgtcctgaaa caatttgccc acctattata ttccaaaaga aagtatgatc cgcattttgt
    13141 tttacagtat atgaccaact cgcattggtg tctaagcaca aactatttgc accattaatt
    13201 gcattattga aaccgggaaa ataaatttcg gtagttgcat aattggtaag taaagcacta
    13261 ttaaaatcaa aatagatgga agcgcgattg aaaatagtat cgcccacatt taaagttttt
    13321 tgtggcttta tacgataccg aatgtatcca tgacttgctg gttcatccac attgctatcg
    13381 ggcaattgaa tatttggaaa ctcaaaagtg gcaagtcggc tttctttgtt aatgctcatg
    13441 tttacaggca cagacgaaga cacgtactcg aaagctgcaa gattcaaacg gtatggaata
    13501 atattttcca cttttacatt gaaggctgta tcgttgccgg tgttttgaaa ataaataaca
    13561 tactccaaat agggcggcac cggttttaaa tcgaaaattg aaaccgaatc ggtgcttaca
    13621 attatttcat tagggtcgaa cgaaccaatt gttgttacct cccaacttgc actattgttt
    13681 gcaatatcat agtcatttac aaagggaaat atttttgcgc tcgaatttat tatcgtacca
    13741 atttgcaaac cttgatgaac gtatacggtt acagtaatat caccctcacc atagggagca
    13801 agtgcgggca aactccaaat tacagagtcg gcataaatat tatcgggcaa cacatttgat
    13861 gaaacaaaat caactttagc atcattgaaa aaataaatag ttggcgacaa aactgttgta
    13921 ccaatatttt tgtaatgaat attgtaagta gccgtaaacc cgctgcggaa aggtgatagc
    13981 ggatgtatgc tgatatataa atcattggca acggtggtag gttgatatgc aaaatcattc
    14041 aacgaatcta tttgctgcat ggcggtgaaa ttcacagtat gtgaatttgg attaggaatg
    14101 taataactta tttgagatgg actcaccaca aaactacccg tatcaaaaac tccaatagaa
    14161 taaaatccat ccgattgaga aaaagtataa cggccattat ttgcctcatt aatttttatt
    14221 cccgatatat ttacctcgcc actatcctga acattattat gattattatc tgcataaact
    14281 tttccagtga tgcggcttga tggggatagt tttactaccc aataatcatg gattccatta
    14341 ctattttcgg ttttgtcacc ggagatattt gaatttgaat cgcctcccaa aacgtaacct
    14401 ccatcgggtg tggaggttat tgaatataat ccgtcactgc tattcccccc aatggtattt
    14461 tgccatacaa tagcaccaag gctatcaatt tttactaccc aataatccca accaccattg
    14521 ctattttctg ttttgtcacc ggagatattt gagtatgacc atcctcccaa aacgtaac
//
LOCUS       DAORQA010000033        13748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1204_length_13748_cov_16.696414, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000033 DAORQA010000000
VERSION     DAORQA010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13748
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1204_length_13748_cov_16.696414"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..830
                     /gene="scpB"
                     /locus_tag="PK736_03950"
     CDS             147..830
                     /gene="scpB"
                     /locus_tag="PK736_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRC32577.1"
                     /translation="MELLKQNIEALIFACENSITIEEIRSCLLTVYGWEISKEDVVAC
                     LAEIKEKFDGEDFSFTLNEIANGYRFLSKPQYYNAIQVFIAQKNKKRLSTAALETLSI
                     IAYRQPISKAEIEHVRGVNCDYSIQKLLEKELIEIEGKSDGPGKPLVYGTSKNFMDYF
                     GLKNPTDLPKLKDIMLPENMIGEPSENADVFEASVVEQEVKEVVDNDDNIETENLTNE
                     TEKPDEEKA"
     gene            979..1776
                     /locus_tag="PK736_03955"
     CDS             979..1776
                     /locus_tag="PK736_03955"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRC32578.1"
                     /translation="MFASIKRYLLFAIIIFNGINVFAHPLSTLRTLPNQAFRPGEVLE
                     YRVHYGFMDAGIAKLEVLPQIETFLNRTCYRLLGTGKSAGAFDWFFKVNDRYESYLDT
                     QAIAPWYFIRRVDEGGYKISQNVIFNHFNNTVKSDKSTLTGPDYLQDMISAFYYARTI
                     DMSKMVAGDIIPIQAWLDDAVIPLNAKIIGREKLKTKFGTFSCIILRPMLQQGRVFKD
                     NEDMTIWITDDKNKIPVRAEARILVGSVKMDLKDFKGLSNPLALVKK"
     gene            2349..3473
                     /locus_tag="PK736_03960"
     CDS             2349..3473
                     /locus_tag="PK736_03960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRC32579.1"
                     /translation="MGKLHMVDLHGQYLKIKSEIDSAIMAVVESTAFINGPEVKSLAT
                     ELQTYLNVKHVIPCANGTDALQIAMMALNLQPGDEVITASFTYVATAEAIALLKISPV
                     LVDVNPDTFNIDPASIEKAITPKTKAIVPVHMFGQCADMEKIMAIAHKHNLFVIEDTA
                     QAIGADYTFSDGRKAKAGTIGHIGCTSFFPSKNLGCYGDGGAMFTNDDTLAAKIRKMA
                     NHGQSEMYIHDEIGVNSRLDSIQAAVLRIKLKHLDEYAAARNRAADYYDKAFANHPNI
                     KTPVRAANSTHVFHQYTLKLIDKNRDALREHLNANNIPHNVYYPLPLHFQKAYQDARY
                     TAGMFPVSEELCKQVISLPMHTELTNEDMDVIVNAVKKGL"
     gene            3615..4928
                     /locus_tag="PK736_03965"
     CDS             3615..4928
                     /locus_tag="PK736_03965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRC32580.1"
                     /translation="MNIAVVGTGYVGLVTGTCFSEMGNHVVCVDIDKEKVEKMRNGVI
                     PIYEPHLDVIFERNIKQGRLKFTTDLAEAIKNAPIIFLALPTPPGEDGSADLSYVLGV
                     ANKLGELITDYKIVINKSTVPVGTAEKVRNAIAANCKVEFDVISNPEFLREGYAVDDF
                     MKPDRVVIGTQSERAKKVMTELYGPFVRQGNPIYFMDERSAELTKYAANAFLATKITF
                     MNEIANLCERVGANVDSIRIGIGSDARIGKRFLFAGIGYGGSCFPKDVQALARTSQDF
                     GYDFKVLTSVMDVNERQKTIIVDKMKAYYNNDLKGKHIALWGLAFKPDTDDIREAPAL
                     YIINDLLEAGATVTAFDPEAMNNVKKVVGDKINYAADEYAALENADCLLIATEWGIFR
                     TPEFDRIEKTLKAKVIFDGRNLYETSRMKELGYYYSSMGREVAAQ"
     gene            4961..5938
                     /locus_tag="PK736_03970"
     CDS             4961..5938
                     /locus_tag="PK736_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC32581.1"
                     /translation="MAKTILITGAAGFLGSHLCDRFIKEGFNVIGMDNLITGSLKNIE
                     HLFPLKQFTFYQHDVTKYVHVAGNIDYILHFASPASPIDYLKIPIQTLKVSSLGTHNL
                     LGVAKNKKARILVASTSEIYGDPQVHPQTEDYWGHVNPIGPRGVYDEAKRFQEAITMA
                     YHNEHAVETRIVRIFNTYGPRMRLNDGRALPAFFGQALRGEDITVFGDGSQTRSFCYV
                     DDLVEGIYRLLMSDYVWPVNIGNPQEITIKEFAEEIIKLTGTDQKIIYKPLPTDDPKQ
                     RRPDISLAQKTLNWEPKVSRQDGLKTTYEYFKNLPQEELFKKEHRFDNL"
     gene            complement(6196..8697)
                     /locus_tag="PK736_03975"
     CDS             complement(6196..8697)
                     /locus_tag="PK736_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC32582.1"
                     /translation="MKQKLTKTIFAILFVVGMANISKAQQSCQAGFAWYSNSFFAVNF
                     VDSSYAGPGDSIIGHFWDFGEPNMLTDTSNLASPIYSYSDSGLYNVCHTIYTLLGCTS
                     TVCQPVTVGGFGCNLVAVISVDTTTNSMTVTANGGTPPYIYNWSNGATTPTITNLAPG
                     TYCVFVMDANGCIFNVCASTFSNACFVNFGVSQQNNIVTLMPYNTADSLYWDFGDGNT
                     YGTTSMGSVSHPYTATGTYNICLTAYSNGNICSQVCSLYYFTYNCSQLCGVLFKDLNG
                     NNIQDPNEPGMPNLYVVIYGSGGQYSAVSDTAGNYCVTLASGTYTVFCYDDSANFFSG
                     YSITFPIDSSPYGAGYYNVTVGVCDTLTGFNFAFMQNSVNITGYIFGDVNANALFDGG
                     DAGIFNQYVTINGKHYYSDNNGKYNAYLPIGTYTIQYVPTTPFNVFALTTPASIVVNA
                     NTQGNTYANNNFGLNTPNGTVDLKVTLSGNNATPNGNVSLFIHVQNLQATATTYDATL
                     TYDDDLVFTSAFPSQTSHNVVLRELLWNMPALGPFQSTTIVANFVCDSFVQMGQILMH
                     NVHINALNDINLANNDDTLYQTVAAPYDPNNKLVVSTNMQQNNWQMVSANNANQEIMY
                     TVNFQNTGTAPAVNVVVIDDLDADLDASSFKLVASSHPCNVVRNGNQLNYSFSNIMLP
                     DSNTNEPASHGYVTYKVNAKNGLLPGTILSDNANIYFDWNASVATNFTNIQLVTTVNL
                     SENYSSTAIGVAPNPTNGLVNFTFTANAGDNVTITIYDAKGSKVQTQLYNTLSSGSQK
                     IMMDCTHLNGLYTVQLQTPTTLHNTKLMVMPSSVK"
     gene            complement(8827..10095)
                     /locus_tag="PK736_03980"
     CDS             complement(8827..10095)
                     /locus_tag="PK736_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRC32583.1"
                     /translation="MISKNRKHSQYKNLGHSDVGNYFRKPKKTRAEVDAENKIGQFFT
                     EGLNKIVWLFHYLTHRFGPKAPYPKYDTKNTGVHTIVNHNHIRTTVGLVFDWATDTYE
                     SDVIASNIASHHPHYTIHGGDTYFVGDDEEIYENFLAPHSSWHRGSHGSFAIAGNHEM
                     YSKGHAFYKNLLPSLGIKNNDGKFDGQQAGYFCLQNDHWRIIGLDTGYNSIGKIPIIE
                     MLPWFAPDCQLHNDIILWLQNVVKINDKNDTRGIVIITHHPYFTTFKGKSDTIAPAIQ
                     LSKIIGTHKPIIWLWGHEHKLSLYAKHQLDGGVTCYGRCMGHGGMPVELEAKHFVPDK
                     SKPAYKKLIAYDNRNSNISSESNLGYNGYAMLDLEKEKLTIKYYDIERYLLAEEWKHN
                     MDGTIEGRMIVDDFCPISTMNDKEWEDAVK"
     gene            complement(10254..>12564)
                     /locus_tag="PK736_03985"
     CDS             complement(10254..>12564)
                     /locus_tag="PK736_03985"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC32584.1"
                     /translation="NVFSDYTIYNPETDKTYKVAIRSKHEKINFCNCLDFKTNGLGTC
                     KHIEYIKQHIESRAINRQVFESNYIPPYSSVYLEYSEERKIKFRIGTENKEEFEKLTK
                     NYFDKNYELKESGYKQFDVFLKEAKKIQNDFRCYDDALSFIIEKREVDKRKSGLEKIL
                     KKNPDFYNTLLKVKLLPYQNHGVTFAVNAGRSLIADDMGLGKTLQAIATAEVMKNLSG
                     IKTVLVICPTSLKYQWKSEIEKFTNSTITIVEGNLLQRELIYKNDTSFYKIVSYNAAI
                     RDVAFINKMQFDLLILDEAQRIKNWQTVTAKSIKNIQTKYCVVLTGTPLENKLEELYS
                     IIQMIDPLKLGAMFRFVYQHQIKADDGTQKVIGYKDLHKVAEILKHNMVRRHKRDVLS
                     DLPSRIDKNLLVPMTEKQLEFYREYEDTVAKLVNKWIRNKFLSEGDRQKLLINLNLMR
                     MVCDSTFIIDQQTRHDTKIEELMNILDEVFEAGDEKVVVFSQWERMTRLVAQELDARN
                     IQYENLNGNVPSKDREKLLSNFRENPHSRVFLSTDAGGVGLNLQSASLLINLDLPWNP
                     AVLEQRIGRIHRMGQKNNVQIINIISANTIEHQMLTKLKFKSSMAAGVLDNGDSTVFL
                     GDSKFTELMKDLQHIVDVKAMPDVTNDNETEKKILDTTPEEKNEAKEFINQLENETEQ
                     VITKEVAQLTLFNEQELEAPQTDVVKQGVTFFENLMATIADEKKTESLINQLIKTDEK
                     TGETSIVIPIKNKEVMQNGFKLLMQLFKR"
     assembly_gap    12565..12574
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12964..13569)
                     /locus_tag="PK736_03990"
     CDS             complement(12964..13569)
                     /locus_tag="PK736_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HRC32585.1"
                     /translation="MNESIRPILIVIAGPNGSGKTSITQKVLQHEWLENCTYINPDTI
                     ANEKFGNWNSPKAVLEAAEFAQQLREDCLNKKEGIIFETVMSSNEKIDFILKAKANGY
                     FIRIFFVGTDSPSINASRIAQRVLEGGHDVPIPKIISRFGKSIVNCCLLAKHVDRLYV
                     YDNSIDHNEPQLLFRSNNGKLAKKYNGIKIWAEEIFNSIQY"
     gene            complement(13566..13733)
                     /locus_tag="PK736_03995"
     CDS             complement(13566..13733)
                     /locus_tag="PK736_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32586.1"
                     /translation="MQSEKGLLMPDREPTEAQLHALMLEVVKEATKKANDAKQKLYQE
                     IHKLVQSQKGK"
BASE COUNT         4243 a         2511 c         2551 g         4433 t
ORIGIN      
        1 gataaaatcc ggcaaaaatg tgctaatttc gtgcgctcaa aaaaaaattg aaactgattt
       61 tattccttcc atatccgctt tagattattt ggttggtttt atattggtta caatgtgtat
      121 cgaaataata ttgaagaaaa attaaaatgg aattacttaa acaaaatatc gaagccttaa
      181 tatttgcctg cgaaaacagt attacaatag aagaaattag aagctgtttg ctcactgtat
      241 atggctggga aatttcgaaa gaggatgtgg tggcatgttt agccgaaata aaagaaaagt
      301 tcgatggcga agatttcagc tttacgttaa acgaaattgc aaacggctac cggtttttga
      361 gcaagccaca atactacaat gctattcagg tttttatagc acaaaaaaac aaaaaacgat
      421 tatcgactgc cgccctcgaa acgttatcga ttattgctta tcgccaacca atatcgaaag
      481 ccgaaataga acatgtacgc ggtgtaaatt gcgattacag tattcaaaaa ttattggaaa
      541 aagaattgat agaaatagaa ggcaaaagtg atggccctgg taagccattg gtgtatggaa
      601 caagtaaaaa ctttatggat tatttcggcc tgaaaaatcc aacagatttg cctaagctta
      661 aagacataat gttacccgaa aatatgattg gcgagccatc ggaaaatgcc gatgtgtttg
      721 aagccagtgt tgtagaacaa gaagttaaag aggttgttga caatgatgac aacattgaaa
      781 ctgaaaatct aacaaacgaa acggaaaagc cagacgagga gaaagcctag ttttttgcca
      841 aaaaagcatc tgcaaataaa ctgcacacat gaattgtagt taaagtaatt attctatctt
      901 tgatttgcct aagaccataa tgatttgtgg aacaacattt gatttttgtt acgcgttatc
      961 atttactaat ataaaaaaat gtttgcatct attaagcgct atttactttt tgccatcatt
     1021 atttttaatg gcattaatgt ttttgctcat ccattgtcaa ccttacgtac cctgcctaat
     1081 caggctttcc gtccaggtga agttttagag tatcgtgtgc actatggatt tatggatgct
     1141 ggtattgcaa agctcgaagt gttgccacaa atcgaaactt ttctcaaccg cacatgctat
     1201 cgtttattag gaaccggaaa atcggccggt gctttcgatt ggttttttaa agtgaatgat
     1261 aggtacgaaa gttacctcga tacacaagca atagctcctt ggtattttat ccgcagggtt
     1321 gatgaaggtg gatataaaat tagccagaat gtaattttca atcactttaa taatactgta
     1381 aaaagcgaca agtctacact caccggaccc gattatttgc aggatatgat ttcggcattt
     1441 tactatgcac ggactattga catgagcaaa atggtggcag gcgatattat tcccatacag
     1501 gcatggctcg atgacgctgt gattcctttg aatgcaaaaa ttattggtcg cgaaaaactg
     1561 aaaacaaagt ttggaacgtt cagttgtatt atactgcgcc ctatgttgca gcaaggtcgc
     1621 gtgtttaaag acaacgaaga catgaccatt tggattaccg atgataaaaa taaaattccc
     1681 gtacgtgccg aagcaagaat acttgtaggt tcggtaaaaa tggacctcaa agattttaaa
     1741 ggattatcta atcctttagc attggtgaaa aagtaatatt gcgaaaacag gttaactttt
     1801 gtcttactat ttctaaacta aaatctccca atatttgtat ttaatagtga taaaagtatt
     1861 caccaaattt cattttctat aaattttaaa cttcgagttt ctttgagcct ttatccatca
     1921 atggcaatga atttatgagt cgactccgaa aaattaattt tgccactaaa tcacaaaggc
     1981 tcaaaatttc actaagttgt aagtgaatca tttttatttt tttgtgggat ttagtgtttt
     2041 ggtgatttag tggccgttta ttagtctaga cttttcggag ttgcctaaaa ttattaaacc
     2101 ctttattcgt tcataaatta ttttctaaat tgattattta tagcaaagct ttaagaagcc
     2161 caaaatactt ttagcacatt attttatttt tttattgtga aaaattaagc ctcatacccg
     2221 agtttttaca ccacgcactt ctcaaataga acaaaaaatt cttagccaaa aatgtcagca
     2281 aacaagccaa taatcgcttt caactaattg attattatat aacttcgccc cctcaaaaaa
     2341 aagaacgaat gggcaagttg catatggttg atttacatgg tcaatatctg aaaattaaat
     2401 cagagattga ctcagcaata atggcggtgg tagaatctac tgcatttatt aatggccccg
     2461 aagtaaaatc gttagctacc gaactacaaa cttacctgaa tgtaaaacat gtaatacctt
     2521 gtgccaacgg taccgatgcg ttgcaaatag caatgatggc gctaaattta caaccgggcg
     2581 atgaagttat tacagcttcg tttacttatg tagcaactgc ggaggcaatt gcattgctaa
     2641 aaatttcgcc agtacttgtt gatgtaaatc ccgatacttt taacatcgac cctgcttcca
     2701 ttgaaaaagc cattacccca aaaacaaaag ccattgttcc tgtgcatatg tttggccaat
     2761 gtgccgatat ggaaaaaatt atggccattg cccacaagca taatttgttt gtaattgaag
     2821 ataccgctca agctattggt gctgattata ctttcagcga tgggcgtaag gcaaaagcgg
     2881 gaacaatagg ccacattgga tgtacgtcat ttttcccatc aaaaaattta ggatgctatg
     2941 gcgatggtgg tgccatgttt acaaacgatg atactcttgc tgccaaaata agaaagatgg
     3001 caaatcatgg tcaatcggag atgtatatac acgatgaaat tggcgtaaac tcgcgcctcg
     3061 acagtataca agctgccgta ttgcgtataa aattaaaaca ccttgatgaa tatgccgctg
     3121 cgcgaaaccg tgcagccgat tattatgata aggcatttgc caaccatcct aatattaaaa
     3181 ctcctgtgcg tgcagcaaac agcactcatg tattccatca gtacacttta aaacttattg
     3241 ataaaaaccg cgatgcattg cgcgagcact taaatgcaaa taatattccg cacaatgtgt
     3301 attatccatt gccattgcat tttcaaaaag catatcaaga tgcacgttat acagcaggca
     3361 tgtttcctgt aagtgaggaa ttatgcaaac aagtaatatc attaccaatg catacagagt
     3421 tgaccaatga agatatggat gtgatagtga atgcagtgaa gaaaggtttg taaggaaaca
     3481 aatatgaagg tttgtgggga cacaaacctt ggacggatga gaatgaatgt ttgtggggac
     3541 acaaacattg gcgcattgag ataataaaat atgaatgttt gtggggacac aaaccttggc
     3601 aaaaaatata aattatgaat atagcagtag taggaaccgg atacgtagga ttggtaaccg
     3661 gcacatgttt ttcggaaatg ggcaaccatg ttgtatgcgt tgacatcgac aaagaaaaag
     3721 tagaaaaaat gcgtaatggt gtgataccaa tttacgaacc gcatctggat gtaatttttg
     3781 aacgcaacat aaaacaagga cgattgaagt tcaccaccga tttggcggag gctataaaaa
     3841 atgcaccaat aattttcctt gcattgccca cacctcctgg cgaagacggc agtgccgatc
     3901 tttcatatgt gttgggagta gcaaataaac ttggcgaatt gattactgac tataaaattg
     3961 taatcaacaa aagtactgtg ccggtgggca ctgccgaaaa agtacgcaat gccattgctg
     4021 ccaattgcaa agtggaattt gatgtgatat cgaatcccga atttttgcgc gaaggatatg
     4081 ccgttgacga ttttatgaaa cccgaccgtg tggtgattgg tactcaatcg gaacgggcaa
     4141 aaaaagtaat gacagaatta tatggcccat tcgtacgtca gggcaatcca atttatttta
     4201 tggatgagcg cagtgccgaa cttaccaagt atgccgccaa tgcatttcta gctacaaaaa
     4261 ttacatttat gaacgagatt gcaaaccttt gcgagcgtgt aggtgctaac gtagacagta
     4321 tacgtatagg cataggaagc gatgcgcgta ttggcaagcg ttttctgttt gccggtattg
     4381 gctatggtgg cagttgtttt ccaaaagatg tgcaggcttt agctcgtaca tcgcaagatt
     4441 ttggttacga ttttaaagtg cttacttccg taatggatgt gaacgaaaga caaaaaacca
     4501 ttatagtaga taaaatgaaa gcctattaca ataatgattt gaaaggaaaa cacattgcat
     4561 tgtggggcct tgcttttaaa cccgataccg atgatattcg cgaagctccg gcattatata
     4621 ttataaacga tttgctcgaa gccggtgcaa ccgttaccgc attcgatccc gaggcaatga
     4681 acaatgtaaa gaaagtggta ggcgataaaa taaattatgc tgccgatgaa tatgctgcac
     4741 tcgaaaatgc cgattgcctg cttatagcaa ctgaatgggg aattttccgc actccggaat
     4801 ttgacagaat agaaaaaaca ttgaaagcta aagttatttt cgatggtcgt aacttatacg
     4861 aaacatcgcg tatgaaagag cttggatatt actacagcag catgggccga gaagtagctg
     4921 cacaataatt tttctatcat atatttttta aatttcacac atggcaaaaa caattttaat
     4981 tacaggtgct gcaggctttt tagggtcgca cttatgcgat cggtttatta aagaagggtt
     5041 caatgttatt ggcatggata accttattac aggttcgcta aaaaatattg aacatttatt
     5101 tcctttaaag caatttactt tttatcagca tgatgtaact aagtatgtac acgtagccgg
     5161 aaacatcgat tatattttac actttgcctc acctgcaagc cctatcgatt atttaaaaat
     5221 acccatacaa acattaaaag taagttcgct cggtacacat aatttattgg gcgtagccaa
     5281 aaataaaaaa gcacgcatat tggttgcctc cacttccgaa atttatggtg accctcaagt
     5341 acaccctcaa acggaagact actggggaca cgtaaaccca atagggccac gcggtgtata
     5401 cgatgaagcc aaacgttttc aagaagctat aaccatggca taccacaacg agcacgcggt
     5461 agaaacccgc attgtgcgca tattcaacac ctacggacct cggatgcgat tgaatgatgg
     5521 ccgtgcattg cccgcatttt ttggtcaggc tttacgtggc gaagatatta cagtttttgg
     5581 cgatggaagc caaacccgtt cgttctgtta tgtggacgat ttggtagaag gtatttaccg
     5641 cttattgatg agcgattatg tgtggcctgt aaacattggc aatccgcaag aaataaccat
     5701 caaggaattt gccgaagaaa taataaaact taccggcacc gaccaaaaaa taatttacaa
     5761 gccactgcca acagacgacc caaagcaacg cagacccgat atttctctcg cacaaaaaac
     5821 actgaattgg gaaccaaaag tttcgcgcca agatggatta aaaaccactt acgaatattt
     5881 taaaaatctt ccacaagaag agttatttaa gaaagaacat cggttcgata atttgtagtt
     5941 ggggtttggt tattgtgttg attggttact ttgttattga actgtttgat ttttggattc
     6001 gtgaatttga gccaattttt ttaaaggatg atcttattca atcattatta atcatcatta
     6061 tcataataat cagcgttcaa aattccaata ctttgtaatc gaaagtgtca aatatcattt
     6121 tcgaaattat aaacaaaaaa aggcgcttca aacgaagcgc ctttttttaa agaacgatat
     6181 tgataccaaa gaaatctatt ttactgatga tggcattacc attaactttg tgttgtgcaa
     6241 tgtagttggc gtttgcaact gaacagtgta aaggccatta agatgtgtac aatccatcat
     6301 tattttttga gaacctgatg atagtgtatt gtaaagttgt gtttgcactt tacttccttt
     6361 tgcatcgtat atggttatgg ttacattatc accagcattt gcagtaaatg taaagttaac
     6421 caacccgtta gtaggatttg gtgctactcc aattgcagtt gaagaataat tctcactaag
     6481 gttaacagta gttaccaatt gtatattagt aaagttggta gcaacagaag cattccagtc
     6541 gaaatatata tttgcattgt cgctcaatat tgttccgggc aataaaccat ttttagcatt
     6601 tactttatac gttacataac cgtggcttgc aggctcgttt gtattgctat cgggcagcat
     6661 gatattcgaa aatgaatagt ttaattgatt gccattgcgc actacattgc atggatgtga
     6721 cgatgccaca agtttaaaac tacttgcatc gaggtcggca tctaagtcat caatcaccac
     6781 aacatttacc gcaggtgctg tgccggtgtt ttgaaaattt acggtataca ttatttcttg
     6841 attggcattg tttgcagata ccatttgcca attattttgc tgcatatttg tcgacactac
     6901 cagtttgtta ttagggtcgt aaggtgcagc tactgtttga tacagtgtat catcgttgtt
     6961 tgcaagatta atatcgttta atgcattgat gtgtacatta tgcataagta tttgtcccat
     7021 ttgcacaaat gaatcacaca caaaatttgc aacgatggtt gttgattgga atgggccaag
     7081 tgcaggcatg ttccacaaca gttcgcgcag tacaacattg tgcgatgttt gtgatgggaa
     7141 agcggatgta aaaaccaaat catcatcata agttaaagta gcgtcataag ttgtagcagt
     7201 ggcttgtaaa ttttgtacat gtataaacaa agaaacattc ccatttggag tggcattgtt
     7261 accactcaaa gtaactttta aatcgacagt gccatttggt gtatttaatc caaaattatt
     7321 attagcataa gtatttcctt gtgtattggc attaactaca atagaggccg gtgttgttaa
     7381 tgcaaaaaca ttgaaaggtg ttgttggtac gtattgaatg gtataagtac ctattggtaa
     7441 ataagcattg tacttaccat tgttatcact gtaatagtgt tttccattta tagtaacgta
     7501 ttgattaaat attccggcat cgcctccatc aaataatgca ttggcattca catcgccaaa
     7561 aatataaccc gtaatgttta cactgttttg cataaaagca aaattgaatc cggttaaagt
     7621 atcgcataca ccaaccgtta cattataata acctgcacca taaggacttg aatctatagg
     7681 aaaagtaatt gaatatcctg aaaagaaatt tgccgaatca tcgtaacaaa aaacagtata
     7741 agtgccactt gcaagtgtaa cgcaatagtt gccggcagta tccgaaacgg cactgtattg
     7801 acctcccgaa ccataaatta caacatataa attgggcatt cccggctcgt tagggtcttg
     7861 aatattattg ccattcaaat ctttaaataa tactccgcat aattgagaac agttgtaagt
     7921 aaaataatat aatgaacaca cctggctgca aatatttcca ttagaatatg ccgttaaaca
     7981 aatattgtat gtaccggtgg ctgtatacgg atgtgatacc gaacccatag atgtggtacc
     8041 ataagtattg ccatcgccaa aatcccaata aagtgaatcg gcagtattgt atggcatcaa
     8101 agtgacaata ttattttgtt gcgagacacc aaagtttaca aagcatgcat ttgaaaaagt
     8161 agaagcacac acattaaaaa tgcaaccatt ggcatccatt acaaaaacac aataagtacc
     8221 gggtgcaagg tttgtaatgg taggtgtagt agccccattc gaccaattgt atatgtatgg
     8281 aggagtgcca ccatttgcag ttaccgtcat cgaattggtt gtagtgtcta cggaaatcac
     8341 tgcaactaag ttacatccaa aaccgccaac ggtaacaggc tggcacacgg tgcttgtaca
     8401 acctaataaa gtataaatgg tatgacaaac gttatataag cctgaatcgg aataggaata
     8461 aattggagaa gcaaggtttg aagtatctgt gagcatgttg ggttcgccaa aatcccaaaa
     8521 gtgtccaatt atagaatctc ccgggcctgc ataggatgag tcgacaaagt ttacagcaaa
     8581 aaaagaattg ctataccacg caaatccggc ctgacatgat tgctgtgctt tactaatgtt
     8641 tgccatgccc acaacaaaca ggatagcgaa aatagttttt gttagttttt gtttcatttt
     8701 ataaattgtt taatgtttta tctatttatc attacgcgaa gtaaataatt tttgtgagtg
     8761 gtgaggaaat gtttttttgc aaagtgctgg caaaaggctt gcaggataaa atttatttgc
     8821 acaatatcac ttcacagcat cttcccattc cttatcattc attgttgata tggggcaaaa
     8881 atcatcaaca atcattctac cttcaattgt gccatccatg ttgtgtttcc attcctcggc
     8941 aagcaggtat cgttctatat cataatattt aatagtgagt ttttcttttt ctaaatctaa
     9001 catagcataa ccgttgtaac ccaaattgct ttcgctcgaa atatttgaat tgcgattatc
     9061 ataagcaatc aattttttgt aggcaggttt cgatttatca ggcacaaaat gttttgcttc
     9121 cagttcaacc ggcataccac catgccccat gcatcggcca tagcaagtaa ctcctccatc
     9181 gagttgatgt tttgcataca acgatagttt atgttcgtga ccccacaacc atataatagg
     9241 tttatgggtg ccaataattt tagacaattg aatggcgggc gcaatggtat cacttttgcc
     9301 tttgaaagtt gtaaagtatg gatgatgagt aataattaca atgccgcgcg tatcattttt
     9361 gtcgtttatc ttcaccacat tttgtaacca taaaataata tcattatgca attggcaatc
     9421 gggcgcaaac cagggaagca tttcgataat gggaattttg ccaatggaat tgtagccggt
     9481 atctaaacca ataattcgcc aatggtcgtt ttgcaaacaa aagtatcccg cctgttgacc
     9541 atcaaatttt ccgtcattat tttttattcc taacgatggt aaaagatttt tataaaaggc
     9601 atgcccttta ctgtacatct catggttgcc tgcaatagca aagctgccat gacttccgcg
     9661 atgccacgat gaatgtggtg caagaaaatt ttcgtaaatt tcttcgtcat cacctacaaa
     9721 ataagtatcg ccaccatgaa tggtatagtg tggatggtgc gatgcaatat tcgatgcaat
     9781 aacatcgctc tcgtaagtat cggtagccca gtcgaaaacc aaacctacag ttgtacgtat
     9841 atggttatgg ttaacaatag tatgcactcc tgtgtttttg gtatcgtatt ttgggtaggg
     9901 agcttttgga ccaaaacgat gtgtgagata atgaaacagc catactattt tatttagccc
     9961 ttcggtaaaa aattgcccga ttttattttc ggcatctact tcggccctgg tcttttttgg
    10021 cttacgaaaa taattcccta catcgctatg gccgaggttt ttatattgag agtgtttgcg
    10081 gtttttggat atcatggttt aattgaataa cctaaaattt attgactgtt ggtactacac
    10141 tatttggaca cgaattacac aaatttgtac gaattaatac gcatagcatt gtttattgat
    10201 aagtaagttc accttcgaat aacaacaata attggtaagc caggcagata aatttatcgt
    10261 ttgaataatt gcatcagtaa tttaaatcca ttctgcatta cctctttatt ttttattggg
    10321 attacaatac ttgtttcgcc tgttttttca tccgttttta tcaattgatt tattagcgat
    10381 tcagtctttt tttcatcggc aattgtggcc ataagatttt cgaaaaaagt aacaccttgc
    10441 tttacaacgt cagtttgcgg tgcttccaat tcttgttcat taaacaaggt aagttgtgcc
    10501 acctcttttg ttattacttg ttctgtttcg ttttcgagtt gattaataaa ttctttggct
    10561 tcgttttttt cttcaggcgt ggtgtctaat atttttttct cggtttcgtt atcgttggta
    10621 acatcgggca ttgcttttac atccactata tgttgcaaat ctttcatcag ttcggtaaat
    10681 ttactatcac ccaaaaacac tgtgctatcg ccattatcga gcacacctgc cgccatcgat
    10741 gatttaaatt tcagctttgt aagcatttga tgttcgatag tatttgccga aataatattt
    10801 ataatctgca cattattttt ttgtcccatg cggtgtatac gtcctatgcg ttgctccagt
    10861 actgcgggat tccagggcaa atcgagattg ataagcaaac ttgccgattg caaatttaat
    10921 cccacgcccc cagcatcggt cgataaaaaa acgcgcgaat gaggattctc gcgaaagttt
    10981 gaaagcaatt tttcgcggtc tttacttggc acattgccat tcaaattttc atattgaata
    11041 ttgcgcgcat caagttcttg agccaccaac cgggtcatgc gttcccactg actaaacacc
    11101 accacttttt cgtcacccgc ttcaaacact tcgtcaagta tattcatcag ctcttctatt
    11161 tttgtatcgt gccgtgtttg ttgatcaata ataaaagtgc tgtcgcaaac catgcgcata
    11221 aggtttaagt tgataagtaa tttttgtcgg tccccctcac ttaggaattt atttcgaatc
    11281 catttattta ccaattttgc aactgtatct tcgtactcgc ggtaaaattc caattgcttt
    11341 tcggtcatgg gcacaagcag atttttgtca atcctgctag gtaaatcgct tagcacatcg
    11401 cgcttatgac gccttaccat attgtgcttc agtatttcgg ccactttatg caagtcctta
    11461 taaccaatta ctttttgcgt gccatcatca gcctttattt ggtgctgata tacaaaccga
    11521 aacattgcac ccaatttcaa tgggtcaatc atttgaatga tggaatatag ttcttcgagt
    11581 ttgttttcta aaggggtacc tgtaagcact acacaatatt ttgtttgaat atttttaata
    11641 ctcttagctg ttactgtttg ccagtttttt atgcgttgtg cttcgtccaa tatcagcaaa
    11701 tcgaattgca ttttatttat aaaagccaca tcgcgtatgg cagcattgta actcactatt
    11761 ttataaaacg atgtatcgtt tttataaatc aactcacgtt gcaacagatt gccttctaca
    11821 atagtaatgg tgctatttgt aaatttttca atttccgatt tccactgata ttttaatgag
    11881 gtagggcaaa taaccaaaac tgtttttata ccggataaat ttttcatcac ctcggcagtt
    11941 gcaattgctt gtagtgtttt tcctagaccc atatcatcgg caataaggct acggcctgca
    12001 tttactgcaa aggttacacc atggttttga tagggcaata attttacttt caataatgtg
    12061 ttatagaagt caggattttt tttcagtatt ttttcgagcc cgcttttacg cttatccact
    12121 tcgcgttttt caattataaa tgaaagtgca tcgtcataac atctaaaatc attttgtatt
    12181 tttttcgcct ctttcaaaaa cacatcaaat tgtttgtatc cactttcttt taattcataa
    12241 tttttatcga aataattttt agtcagcttt tcaaattctt ctttgttttc ggtaccaatg
    12301 cggaatttta ttttccgctc ttcgctgtat tccaagtaaa cagatgaata tggcggaata
    12361 taatttgatt cgaacacctg ccggtttata gcgcgcgatt caatatgttg ttttatgtat
    12421 tcgatatgtt tgcatgtgcc taaaccattt gttttaaaat cgaggcaatt gcaaaaattt
    12481 attttttcgt gcttactgcg tatagcaact ttgtaagttt tatctgtttc gggattgtaa
    12541 atagtgtaat cggaaaacac atttnnnnnn nnnngcctat attttttact tcaaattttt
    12601 tcatttcggc aaactgtcta cgcaaagcaa tttgccatgc atcaatactc atctcttttg
    12661 gctgctgata gtatgatact tttggtgtgg cttgagttat cttcattgca actttttttt
    12721 tggttacgtt catatttttt ttatcttagt aataatgcag ggctacttaa gtatgccaac
    12781 attcaggcac gaattacaca aattaaaatt tataatattt aagttcaatg ttttttgtac
    12841 ttctcaggaa aattgcatca ctgatgaaca cagataaatt catagactta caaaaaaaat
    12901 gtgcgatatt aaatgtgcga aaatttacgg gaaattattt atctactaaa ttttttgcat
    12961 cgcttaatat tgaatagaat tgaaaatttc ttcggcccaa atttttattc cattatactt
    13021 ttttgccaac ttgccgttat tgcttctgaa taataattgc ggctcgttat ggtcaataga
    13081 attatcataa acataaagcc tatcaacatg ctttgcaagc aagcaacaat ttacaattga
    13141 ttttccaaat cgtgaaataa tttttggaat tggaacgtca tgtcctcctt ctaacacacg
    13201 ttgtgctatc cgacttgcat taatggatgg cgaatctgtt cccacaaaaa aaatccgtat
    13261 aaaatatcca ttggccttag ccttcaaaat aaaatctatt ttttcattcg aagacattac
    13321 tgtttcaaaa atgattccct cttttttatt taaacaatct tcgcgcaatt gctgtgcaaa
    13381 ttctgctgcc tctaatacgg ctttaggtga attccaattg ccaaattttt cgtttgcaat
    13441 tgtatcaggg ttaatatatg tacagttttc aagccactca tgttgcagca ctttttgtgt
    13501 aatagatgtt ttgccactac catttggtcc agctataact attagtatgg gtcttatact
    13561 ttcattcatt ttcctttttg cgactgtacc aatttatgaa tttcctgata aagtttttgc
    13621 ttcgcatcat tcgctttttt tgttgcttct tttacaacct caagcatcaa agcatgtaac
    13681 tgggcttcgg taggttcacg gtctggcatt aataatcctt tttcgctttg catttatttg
    13741 tatttgtt
//
LOCUS       DAORQA010000034        13491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1242_length_13491_cov_8.920512, whole genome shotgun sequence.
ACCESSION   DAORQA010000034 DAORQA010000000
VERSION     DAORQA010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13491
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1242_length_13491_cov_8.920512"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PK736_04000"
     CDS             complement(<1..940)
                     /locus_tag="PK736_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32587.1"
                     /translation="MKKNLLSLLLATTSILMLSINENAYSQCKWQQKLYDGYEYSTTV
                     PDLIPGTTIQTTPQSFAVHSGTKSLYMNFINTLPGGSLVYDRTITVCANVPIQISAWL
                     TTSFSGVQCDARIEIVDANNIQLANTPSILCSYAPIWTQYQSGSITPTTSTIRFKLYT
                     NAPGSPGGNDLSFDDLLVEYCNAMELGNDTSLCNPSTLLLNAGSGYSSYLWSNGSTNQ
                     TLLATSIFPGTTSYTYYVTAQDSNGCTFKDTINILFVNCASVNELNQTVKYTVQPNPA
                     NDYIVIDIDSDLLEVKLFNAIGEKIKSFSNTKFFNVE"
     gene            1230..1646
                     /locus_tag="PK736_04005"
                     /pseudo
     CDS             1230..1646
                     /locus_tag="PK736_04005"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            1697..>3158
                     /locus_tag="PK736_04010"
     CDS             1697..>3158
                     /locus_tag="PK736_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRC32588.1"
                     /translation="MRKFLYIVTLTVIFTQAKAQRNVILIVADDLGIDYCGFYENHKD
                     TIAMPNVRKLLAKGIRFKNAWVNPVCSPTRAGMLTGRFSFRTGVGDYITDSNSVVLDT
                     AEITIPKVLKGLGGSSIATAQIGKWHLQHVQPTTNYIFPNTMGFDNFEGSFGAQVPNY
                     YNWPKITNGVSKNCKTYATIENVDNAVKWIKTQQSKPFFLWLAFNAGHTPLHLPPPGM
                     YSDSTLSGTPGNIAAYPKKYFKASIEAMDKEIGRLFDSLQVLNKLDSTDFIFVGDNGD
                     NPPVAQGTTNAKGTIYQGGIAVPFIIAGPSVVNPGRTSNALVCGQDIFATVIDLFGFS
                     NWKNLITINKPIDSKSIMPIIKNTSTSIQPWIFTEIFRVNPSTRDGRTIRNSHYKLLS
                     VDNGTQKMFRIFGDPSEKTNLLDGDLTATELINYNYLCSEMSNLTGLSGSCNSNVDLP
                     KITINTITTNVCVNAIVTFNANASKAGVAPIYQWKKN"
     assembly_gap    3159..3187
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3188..4075
                     /locus_tag="PK736_04015"
     CDS             <3188..4075
                     /locus_tag="PK736_04015"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32589.1"
                     /translation="ANNDAISCVITTTTSASASSNIIKMNVFSGVPNVPAWISGARYD
                     LCNTTTDYVYEVSAVSNATGYNWTVPNDAFIKSGQGTSFITVNFLPSFRLGNITVNGM
                     NACGGGGVKVANISANPSKPSVINGINQLCNNQQNVSYSIDSMALVTGYLWAVPTDAT
                     IVAGQGTNNIVVNFGIKTGNITAQAQNNCGGSIKSVLPVNFNCRLSQSFNSSNNNSFS
                     VVSPFDKEIIFTSDQSINHAIATLSDVTGRVVYNWGNISFNANEIMKLQMDKNIPKGI
                     YFFKINNTSFDFVAKVSHE"
     gene            4241..6865
                     /locus_tag="PK736_04020"
     CDS             4241..6865
                     /locus_tag="PK736_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRC32590.1"
                     /translation="MKSLLCIIVLSFFSFAVYSQRNVVLIIADDVGTDWFGFYEDHVD
                     TASMPNIRKLLSKGVRFQNAMSNPVCSPTRAGIFTGRYSFRTGVGNVVGLAGAGELDT
                     AEITIPKLLKIYNPNIGKAQMGKWHLQSATSASHLMYPNKMGYNYFAGSFPGQLTNYY
                     NWTKITNGVSSTSTTYATTENTNNAITWIKSQNNKPFFLWLAYNAAHTPYHVPPMGFY
                     NGTLSGTQQDINANPKKYFKASLEALDHEVGRLFDSLAVLNKLDSTDFIFIGDNGNTQ
                     NTAQVANTTRAKGTIYQYGVHVPFIVSGPSVINPGRVSSALVNTVDIFATVLELFGYS
                     TWQSQISVNKPVDSKSLMPIILNQSTSVRPWAFTELFKTTADPKAGKAMRNIDYKLMN
                     FDDGHQEFYNLTNDYSEANNLLLGTLSNTDIVNYNYLCQEMSTLVGAGNYCNAAYSLP
                     TVEITSSATSICSGTNVTFSATITNGGTSPIYQWKKNGTNVGTNSNTYSTTSLTNGDT
                     IKCVLTTASLTTATSNSIAITVIVNVTPAISITASSSTICTATSVSFNATVTNGGTSP
                     SYQWKKNNANVGNNSSAYTDIALANGDVVSCTLTSNAVCANSSTANSNSITMAVSNAI
                     PAKPNSIAGTKFNLCNTSTNYTYSIAAVSGATSYIWSVPANCSLISGQGTPSIVVAFS
                     SQFKSGTLSVSAVNGCGVGIARTAGLYAATAKPGTINGLSVLCANQQSVTYSIPAVTG
                     ATSYKWTVPTGATIASGQGSTAIVANFGTSSGLVTVVAKNACASSSKSSFTITMNCRM
                     GDDMYDDNISIVTVVNPFEDKIIVNVNTDIDNAEVTLYDITGREIYCWKNLSMKTNTQ
                     QHFDVEKKFTQDNLFSLH"
     gene            6944..8536
                     /locus_tag="PK736_04025"
     CDS             6944..8536
                     /locus_tag="PK736_04025"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRC32591.1"
                     /translation="MNKYLIAFCFFIFSCDTYAQRNVILIIADDIGTDYFGFYEDHND
                     TVDVPNIRSLMNNGIRFKNAMSNPVCSPTRAGIFTGRYSFRTGVGGIVGGGGGSNPLD
                     TAEISIPKLLNVYNLNIVKAQIGKWHLNPAMPQSNLQIPLALGYNHFEGPFTGALTNY
                     TNWTKYTNGVASTITTYATTENVNNAMSWINNQTGKPFFLWLAFNAPHSPYHLPPNNL
                     HSYVLSGTPQDIMANPKNYFKASLQALDTEIGRMIDSLKAINRFDSTDFIFVGDNGNT
                     SQTAQIANINRAKGTIYQYGVHVPFIISGPSVINKGTTSDALINTVDIFATVNELMGN
                     TNWQAQIPANKPVDSKSIVPILKNQSSTIRPWSFTEIFKLTPDANDGKAIRNMDYKLL
                     RFDDGHEEFYCLANDSLELNDLLPGTLNGIEISNYNYLCSEITTLLGIGAMCNYTSFH
                     QLENNSDELSAYPNPFTSFIYFQNKIEKNYYQLIDYTGKIIYSGDNIEAQNFSHLRQG
                     IYLLQTTDVVTHRTSTQKLVKQ"
     gene            8712..11042
                     /locus_tag="PK736_04030"
     CDS             8712..11042
                     /locus_tag="PK736_04030"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRC32592.1"
                     /translation="MFSRITLFLAIFCGGIFSAINTKAQCCTYTLSMHDSYGDGWNGG
                     QLEIFINNISAGIFSGKNYASVDTFTICNGDSLVLFYTAGMYENENSYELFDPAWNLI
                     FDDGPTPQIDTVFNYSGNCNVISLAGSHPCNAIPIDTVNCIFADNSIFAGSGLNAGCA
                     DYKGHDMWFSMVVPPSGNISFATDSGNINDTGIAAWTDSLCTNLKNIGCDDDGGPGYY
                     SFLLLYDLTPGSTVYIQVFGYGGGTGTFQLCAHDLGKVILDSSELPIVILHTQGQQII
                     EDKKINALMEIIYNGANNITYVNDSANIYNGNIGIEIRGATSASYPQRPYGLETRDTS
                     GANNDVSILGMPAENDWVLLSNFNDRSLIRNPLAFKLFGDMGNYSVRSQLCEVLVDTN
                     YKGIYMFGEKIKRDSGRVHISKLTAIDTTGDELTGGYILQQNYWDATNSFQSNYSPID
                     HPTFDVHFVYEYPSADTIAVSQRNYIAAFIDSLEDALYSPNFDDTLIGFRKYLDVKSF
                     IDYFLINELARSNDGFKKSVFFYKDKFSKGGKLKAGPVWDFDWGFKILYGCINTENSI
                     GAGWAHLVNDCPTDNYSTGWYIRMLQDSTFNNELRCTYEDYRANILDTAVIFAYIDSV
                     RNVVQNAQARHFRKWPILGISGPAPEINPIATTYNAELDTLKKWISIRLQWLDANIPG
                     LCIPVFTSTNESNNFSRFNFFPNPTNGIVHFDGGVFQNREVIFQIYSCDGKLIEQKNF
                     LDGKIKFDFFLKEKGVFYFTFTNSNGILQRGKIVVI"
     gene            complement(11191..12942)
                     /locus_tag="PK736_04035"
     CDS             complement(11191..12942)
                     /locus_tag="PK736_04035"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32593.1"
                     /translation="MIILIYTLVLTKFFKTMLKKSYLTLVSIFAFCFYAKAQQPMSSP
                     TSLRNDITITRIMGIPYEGVTRMVYNKIDSNLYYATYDGDIRVIKNFLQPYRTDSLLF
                     TGSNHGIQVVYGLLVKNNSIYVSGSNLANKYYKSYIKKATLQNNGSYTWSTVASTASY
                     AGGKYFNHLFSGITFNPAQDTIVVCSGSRGDHGEIDSFDGTFPNKRGVPLAAIILKVP
                     ADSINIILPNNVTTLNSQGRLVAKGIRNTYDFAYNAKGELFGAENSSDADHEDEINWI
                     RQGKHYGYPWVLGSTWNPQYFSSFNPANNVMVPKTSYSYINNQWKNDPTFPVPTQKFI
                     LPCANNGPDADMMRDTATGAIIDASALGKKCYSLTSHRSPLGILFDTDSLIGCDLKGD
                     GFVLSFTRGNAGLPKPSSLLTPFNDNSEDLLQLEFTKNNTKNVYEFTAKKIVTNFWHP
                     VDAVQIENSIYVMETGDSTEASIWKVELPAKNACPVQPVVSEEKIFLNTDENYEEQKV
                     LLFPNPAYDILYIDNMDNAENLQMMIYNSTGQLILKRILEEGITTIDTDKIAAGLYNI
                     IIFDDNNFLYRETIVIE"
     gene            complement(13147..>13491)
                     /locus_tag="PK736_04040"
     CDS             complement(13147..>13491)
                     /locus_tag="PK736_04040"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HRC32594.1"
                     /translation="TLLAAVKTIGEYDMQYFIVVSAASVTILTSALKTFQYQELWVNY
                     RSTIEQLKPEIFYYKFDSGEYGVPGIDKEKLFVTRVESILNKEHDVWPVAKKTNEQGD
                     KKDAKDKTTETK"
BASE COUNT         4239 a         2461 c         2581 g         4181 t
ORIGIN      
        1 cttccacatt aaaaaacttt gtgtttgaaa acgattttat tttttcacca atggcattaa
       61 ataattttac ctccaataaa tcgctgtcaa tatcaattac aatataatca tttgccgggt
      121 ttggttgcac ggtatatttt acggtttgat taagttcgtt tacagaggca caattcacaa
      181 ataaaatatt gatggtgtct ttaaaagtac aaccattaga atcctgagca gtaacatagt
      241 atgtatacga tgtagtgccc ggaaaaatgg acgtggctaa caatgtttga ttggtactgc
      301 cattgctcca taagtatgat gaataaccgc tgcctgcatt aagcagcaat gttgatgggt
      361 tgcacaaaga ggtatcgtta cctaattcca tagcattgca gtattcaacc agcaaatcat
      421 caaacgacag gtcgttgcca ccggggctgc cgggagcatt ggtatacagt ttaaatctta
      481 tagtgcttgt agtaggagta atgcttcccg attgatactg tgtccaaata ggtgcataag
      541 agcaaagtat acttggggtg tttgcaagct gtatattatt ggcatcaact atttcgatgc
      601 gcgcatcgca ttgtacaccc gaaaaagatg tggtaagcca ggcagaaatt tgaatgggta
      661 cgttagcaca aacggtgata gtgcgatcgt aaaccagcga cccaccggga agcgtattta
      721 taaaattcat atacaacgat tttgtgcctg aatgcactgc aaaactttgt ggtgttgttt
      781 gtatggttgt gccaggaata aggtcgggaa cagtagttga atattcatat ccgtcatata
      841 acttttgttg ccatttacat tggctgtagg cattttcatt tatcgacaac attagaatgc
      901 ttgttgtagc caataacaat gagagtagat tttttttcat ttttttagtt taaattaatt
      961 ttatgtttta agtcgaaaga agttttttat cctattgcaa aggaacaaaa ttttcactgt
     1021 cgtgatggca aatttactcg atttttattt ttagaaattc gaaaccattt atattttgat
     1081 tgattgatgc caaaggtttt attattgtat ttataccgaa acaacttagg ttttggagaa
     1141 acaagctatg aatttgttcg gtactactaa gagtacgttg tattttcatt cgaaaatcta
     1201 attactctta gtatacatgt aatcattgtc cgtttgcaaa attgtatcct caacgattga
     1261 atgcccttaa taaaaagtac agtgaatcag gcgttccatt aattgcaata agttcaaccg
     1321 atactgttgt ttacgatgaa gatactttta gcaacatggt aataaaatcg agagatgaaa
     1381 aaattaatta tccttatctt tttgatgaaa tgcaaactgt ggcaaaaaat tttggcgcac
     1441 aaaaaacacc tcatgcgttt gcaatatgga accaaaatgg aaagtttgtt gtaaaatata
     1501 atggtgctat agacgataat ggtgccgagc ccgaaaaagt tacatcccac tatgttgcag
     1561 aggcagtaga tagtttactt atgcgcaagc aggtaaaaat aaacagtact ttttcaatcg
     1621 gttgtcaaat acattttcgt aaataataaa tagaaatatt ttttagtttt gtgccggaaa
     1681 cacattcaaa ataattatga gaaagttttt atacatcgtt accttaactg ttatttttac
     1741 gcaggcaaag gcacaacgca atgtgatatt aatagtggca gacgatttag gaattgatta
     1801 ttgcggcttt tacgaaaacc ataaagatac tatagcaatg cccaatgtac gtaaattgct
     1861 tgcaaaaggc ataaggttta agaatgcatg ggtaaatcct gtatgctccc caacccgtgc
     1921 tggtatgctc acagggcgtt tcagctttcg aacaggagta ggcgattata ttaccgattc
     1981 taattcggtc gttttggaca cagccgaaat tactatcccg aaagtgctta aaggtttggg
     2041 tggaagttca atagctaccg cccaaatagg taaatggcac ttgcaacatg tacagcctac
     2101 aacaaattat attttcccta atacaatggg tttcgacaat ttcgaaggat cttttggtgc
     2161 tcaggtcccc aattactata actggccaaa aattacaaat ggagtatcga aaaactgcaa
     2221 aacatatgca acaatcgaaa acgttgacaa tgcagtgaaa tggataaaga cgcaacaatc
     2281 caagccgttt tttttgtggc tcgctttcaa tgccggccat acgcctttgc atctgccacc
     2341 acccggaatg tattctgatt ctactttgag tggcactccc ggaaacattg ccgcatatcc
     2401 taaaaaatat ttcaaagctt ctattgaagc tatggataaa gaaatcggga ggctattcga
     2461 ttctttacaa gtattaaata aacttgatag caccgacttt atttttgttg gcgataatgg
     2521 tgataaccct cccgtagcgc aaggcactac aaatgcaaaa ggcacaattt atcagggagg
     2581 aattgccgtg ccatttatta ttgccgggcc tagtgttgta aatcccggcc gcacaagcaa
     2641 tgcccttgtt tgtggtcagg atatttttgc taccgtaatt gatttgttcg gcttttcaaa
     2701 ttggaaaaac cttatcacaa ttaataagcc tattgatagc aaaagtatta tgccaataat
     2761 aaagaatact tcaacgagca tacagccttg gatttttaca gaaatattca gggttaatcc
     2821 gtcaacgcgt gatggcagaa ccatccgtaa ttctcattat aaacttttaa gtgttgataa
     2881 tggcactcaa aaaatgtttc gcatatttgg tgatcccagt gagaaaacaa accttctcga
     2941 tggagattta acagcaacag aattgataaa ctacaattat ctatgtagcg agatgagcaa
     3001 cctcacaggc ttaagcggca gttgtaatag taatgtcgat ctgccgaaaa ttaccattaa
     3061 tactataacc accaatgtat gtgtaaatgc aatagttact tttaatgcaa atgcatcaaa
     3121 agcaggtgta gcaccaatat atcaatggaa aaagaacgnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnngcc aataacgatg ctatcagttg tgtaataaca acaactacat ctgcttctgc
     3241 cagctcaaac attattaaaa tgaatgtatt ttcgggagta cctaatgtgc ctgcatggat
     3301 ttccggtgca cgctacgacc tatgcaacac tacaacagac tatgtatacg aagtaagtgc
     3361 agtgagcaac gccacaggat acaactggac ggtgccaaac gatgcattta ttaaaagcgg
     3421 acaaggtaca tcctttatta ctgtaaactt tttgccttca tttagattgg gcaatattac
     3481 tgtgaatggt atgaatgcat gcggtggtgg tggtgtaaaa gttgccaaca taagtgcaaa
     3541 cccatcaaaa ccatctgtta taaatggaat aaaccaatta tgtaataacc aacaaaatgt
     3601 ttcatattct atcgacagta tggcactggt aaccgggtat ttatgggcag tgccaacaga
     3661 cgctactatc gttgccggac aaggaaccaa taatattgtt gtgaatttcg gcattaaaac
     3721 aggcaatatt accgcccagg ctcagaacaa ttgtggcggt agcataaagt cggtattgcc
     3781 tgtcaatttt aattgcaggc tttctcaatc tttcaattcc tccaacaata attctttcag
     3841 cgttgtgagt ccattcgata aagaaattat ttttacttcg gatcaaagta ttaatcatgc
     3901 tattgcaact ttgagtgatg ttaccggtcg agtagtttat aactggggaa atatttcttt
     3961 caatgcaaat gaaataatga aattacaaat ggataagaat attcctaagg gaatttattt
     4021 tttcaaaatt aataacacct ctttcgattt tgttgcaaag gttagtcatg aatagttctt
     4081 cttaattgca aatctttaaa gttttgtaag atttcatatt gggttattcc ttttcttatt
     4141 tggcttaact ggatttaaat tcgtgatggt aaaataattg ccatgcaaat ttaaaccttt
     4201 gttattcgta ttaatctacc taagaataaa acctaccatt atgaaaagtc tactctgcat
     4261 tattgtattg tccttttttt ctttcgctgt ttattcacaa agaaatgtag tgctcatcat
     4321 tgccgatgat gtgggtactg actggtttgg tttttacgaa gaccatgttg acactgcaag
     4381 catgcctaac attcgaaagt tattgagtaa gggcgttcgg tttcaaaatg ccatgagcaa
     4441 tcccgtttgt tcacctacac gtgcaggcat ttttacaggc cgatatagtt ttcgtactgg
     4501 cgtaggtaac gttgtaggac tggccggtgc aggcgaattg gatactgcag aaataaccat
     4561 cccaaaatta ttgaagattt ataatcccaa tattggaaaa gcacaaatgg gaaaatggca
     4621 tttacagtct gctacatcgg catcgcattt aatgtatccc aacaaaatgg gttataatta
     4681 ttttgccggc agttttccgg ggcaacttac caattattat aactggacaa aaatcaccaa
     4741 tggtgtttcg agcacgagta ctacttatgc aactactgag aatacaaaca atgccatcac
     4801 atggataaaa tcacagaaca ataaaccctt ctttttatgg ctggcatata atgcagcgca
     4861 tacgccatat cacgtgccac caatgggttt ttacaacggt actttatcgg gcacacaaca
     4921 ggatataaat gccaatccaa aaaaatattt taaggcttcg ctcgaagcac tcgatcacga
     4981 agttggaagg cttttcgatt cgcttgccgt tttaaataaa ttggacagca ccgattttat
     5041 ttttattggt gataatggca atacgcaaaa cacagcacaa gttgctaata ctacaagagc
     5101 caaaggaacc atctatcaat atggcgtgca tgtgcctttt attgtttcgg gtccatcagt
     5161 tattaacccc ggacgagtga gtagtgctct ggtaaatacg gtggatattt ttgctactgt
     5221 acttgagttg tttggttatt caacatggca atcgcaaatt tctgtaaata aacctgtgga
     5281 tagcaaaagc ttaatgccaa taatattaaa tcaatctaca agtgttcgtc cctgggcatt
     5341 taccgaatta tttaaaacaa cagccgatcc taaagccggt aaggcaatgc gaaatattga
     5401 ttacaagctt atgaattttg acgatggtca tcaggaattt tataacctta ccaacgacta
     5461 tagcgaagca aataatttac tcttaggcac tttgtcaaac actgatattg taaactataa
     5521 ttatttgtgt caggaaatga gcacactggt tggcgcagga aattactgca atgcagcata
     5581 ctcacttcct actgtcgaaa taacatcgag cgccacttct atttgctccg gaacaaatgt
     5641 tacattcagt gccactatta caaatggggg aacctcgcca atatatcaat ggaaaaagaa
     5701 tggaaccaat gtaggtacaa atagcaacac ttattcaacc acttcactaa cgaatggaga
     5761 tacaataaaa tgtgtactta ccaccgcctc attaactaca gcaacatcga acagtattgc
     5821 cataacggtg attgtaaacg ttactccagc aatttcgatt actgctagtt caagtactat
     5881 ttgcactgca acatcagttt catttaacgc aactgtaacc aacggaggaa catcgccatc
     5941 ctatcaatgg aaaaaaaata atgctaacgt tggaaacaat agtagcgctt atacagatat
     6001 tgctttagcg aatggtgatg ttgtttcttg cacactcaca agtaatgctg tatgcgcaaa
     6061 cagtagtact gcaaattcta attcaattac aatggcagtg agtaatgcta tacctgccaa
     6121 gccaaattca attgcaggaa caaagtttaa tctatgtaat acgagcacaa attatactta
     6181 ttcaattgca gctgttagtg gtgctacaag ttatatttgg agtgtgcctg caaattgtag
     6241 tttgataagt gggcagggta ctccttcaat tgttgttgcc ttttcatcac aatttaagtc
     6301 gggcacactt tcggtttcgg cagtgaatgg ttgtggggtg ggcattgctc gtaccgcagg
     6361 attatatgct gcaaccgcaa aacccggaac tattaacggc ctaagtgttt tgtgcgcaaa
     6421 tcagcaaagt gttacttatt ctataccagc ggtaacaggt gcaacaagtt ataaatggac
     6481 ggtgccaaca ggcgctacaa ttgccagtgg gcaaggcagt acagcgatag ttgcaaattt
     6541 tgggaccagt agcggacttg taacagtagt tgcaaaaaat gcttgtgcca gtagttcgaa
     6601 atcatctttt acaataacaa tgaattgcag aatgggcgat gatatgtatg atgacaacat
     6661 tagtattgta actgtagtaa atccgtttga agataaaata attgtgaatg taaacactga
     6721 tatagataat gctgaagtta cattatatga tattactggt cgtgaaattt attgttggaa
     6781 aaatttatcg atgaaaacaa atacgcaaca acattttgat gtggagaaaa aatttaccca
     6841 agacaattta ttttctctcc attaaaaatg aaagcatcaa catcgtttca aaaatattta
     6901 acaagtaata ttacgctttc caattccacg aaagattttt aaaatgaata aatatttaat
     6961 tgctttttgt ttttttattt tctcatgtga cacttatgca cagcgcaatg tcatacttat
     7021 aatagccgat gatattggca ccgattattt tggtttttat gaagaccata atgataccgt
     7081 tgatgtaccc aacattcgta gtttgatgaa taatggtatc cgatttaaaa atgccatgtc
     7141 taaccctgtt tgctctccaa cgcgtgcagg catttttact ggtcgatata gttttcgtac
     7201 tggtgtgggt ggtatagtag gaggaggagg aggttcgaat ccgttagata ctgccgaaat
     7261 ttcgatacca aaattgctca atgtttataa cctaaatatt gtcaaagccc aaatcggcaa
     7321 atggcatttg aatcctgcca tgccacaatc taatttgcaa atcccacttg cattaggata
     7381 taatcatttc gaagggccat ttacaggagc attgacaaac tacaccaact ggactaaata
     7441 tacaaacgga gtagcaagta ctatcaccac ttatgccacc accgaaaatg taaacaacgc
     7501 catgagttgg attaataacc aaacaggaaa accatttttt ctttggcttg cttttaatgc
     7561 tccgcactca ccatatcatt tgccgccaaa caatttgcat tcctatgtgc ttagtggtac
     7621 accccaagat attatggcta atccaaaaaa ttatttcaaa gcatcgttac aagcgctgga
     7681 tacagagata ggccgaatga tagattcgct caaagccata aacagatttg acagcaccga
     7741 ttttattttt gttggagata atggtaacac cagtcaaact gcgcaaatag caaatatcaa
     7801 tcgtgccaag ggtactattt atcagtatgg tgtgcatgtg ccgtttatta tttctggacc
     7861 ttcggtgatt aataaaggaa caacaagcga tgcattgatt aacaccgtgg atatttttgc
     7921 taccgtgaac gaactcatgg gcaatacaaa ttggcaagca caaattcctg caaacaaacc
     7981 tgttgatagc aagagcattg ttcccatact aaaaaatcaa agttctacta ttcgcccatg
     8041 gtcatttacc gagatattta aattgacacc cgatgccaat gatggcaaag ctatacgcaa
     8101 tatggattac aagcttttgc gattcgatga tgggcacgaa gagttttact gccttgcaaa
     8161 cgattcgtta gagttgaacg atttgctgcc tggtactttg aacggaatag aaatttcaaa
     8221 ctataattat ttgtgcagcg aaattacaac actacttggc attggtgcaa tgtgcaatta
     8281 cacttcattc catcagctgg aaaataatag cgatgagttg tcggcatatc caaatccatt
     8341 cacatcattt atttattttc aaaataaaat cgagaagaat tattatcaac taatagatta
     8401 caccggcaaa ataatctata gtggcgataa tattgaagcg caaaattttt cacatctacg
     8461 gcaaggaata tatttgttgc aaacaactga tgtagtaaca catcgcacat caacacaaaa
     8521 acttgtcaag caataaaaat aacatttcag agtcccaact ctttattgtt atctagcttt
     8581 agagtctctc atgccaataa aaaacaaaag cgtatgagga ctctaaaaag atttatgggg
     8641 aggcttttct ttttttgtac tattgccata tatcaaatgg atagtacgaa atgtcaaaca
     8701 aaatgctcac catgttttca agaataacat tattcctcgc aattttttgt ggaggaatat
     8761 ttagtgccat aaacacaaaa gcacaatgct gcacttacac tttatcaatg cacgatagtt
     8821 atggtgatgg atggaatggt gggcaactgg aaatttttat aaacaatatt tctgcaggaa
     8881 tttttagcgg aaaaaattac gccagtgtcg atacatttac aatttgcaat ggcgattcat
     8941 tggtattgtt ttatactgcc ggaatgtatg agaacgaaaa ctcttacgaa cttttcgacc
     9001 cggcatggaa tcttattttt gatgacggcc ccacaccgca aattgataca gtgtttaatt
     9061 atagtggtaa ttgcaatgtg atttcattag caggcagcca tccatgcaat gctattccca
     9121 tcgatacggt gaattgcatt tttgccgaca acagcatctt tgccgggtcg gggctcaatg
     9181 ccggttgtgc cgattataaa gggcacgaca tgtggtttag catggtggtg ccgccttcgg
     9241 gcaacattag ttttgctaca gatagcggaa acataaacga caccggaatt gccgcctgga
     9301 ccgatagcct ttgtacgaat ttgaaaaata ttggttgcga tgatgatggt ggccctggat
     9361 attattcttt cttattgttg tacgacctta ctcccggatc gactgtctac atacaagttt
     9421 ttggatatgg tggtggcact ggaacatttc aattatgtgc gcacgattta ggaaaagtaa
     9481 tactcgatag cagcgaattg cccatcgtaa ttttacacac acaagggcaa caaattattg
     9541 aggataaaaa aataaatgcg ctgatggaaa taatatataa tggcgcaaat aatattactt
     9601 atgtgaatga tagtgcaaat atttacaacg gaaatattgg tattgaaata agaggtgcta
     9661 cttcggcatc gtacccgcag cgtccttatg gtcttgaaac gcgcgatact tcgggggcaa
     9721 ataatgatgt ttcaatattg ggaatgcctg ccgaaaatga ctgggtgttg ttgtcaaatt
     9781 tcaacgaccg ctcgctaata agaaacccat tggcttttaa attgtttggc gatatgggaa
     9841 actattcggt gcggtcacaa ttgtgcgaag tgcttgtaga tacgaactat aagggcattt
     9901 acatgtttgg cgaaaaaata aaacgtgata gtggaagggt tcatatttcg aaattgactg
     9961 ccatagatac taccggtgac gaacttacag gaggatatat tctgcagcaa aattattggg
    10021 atgctaccaa tagttttcaa tctaattatt ctcctatcga ccatccgact ttcgatgtgc
    10081 attttgttta cgagtatcca agtgccgata caattgcagt gtcacaaaga aattacattg
    10141 ccgcttttat cgatagtttg gaagatgcat tgtacagccc taatttcgat gatacattaa
    10201 ttggtttccg aaaatacctc gatgtaaaat cctttatcga ttactttctg ataaacgaac
    10261 ttgcccgcag taacgatggc tttaaaaaga gcgtattttt ttataaagat aaattttcga
    10321 aaggtggaaa acttaaagcc ggcccagtgt gggatttcga ttggggtttc aaaatcctgt
    10381 acggttgcat caataccgaa aactcgatag gtgccggatg ggcgcacctt gtgaacgatt
    10441 gccctaccga taattacagt accggttggt atatacgtat gttgcaggat agtacgttta
    10501 ataacgaact gcgttgtact tacgaagact accgtgccaa tattttagac actgccgtta
    10561 tttttgcata tatagatagc gtgcgaaatg tggtgcaaaa tgcacaggcg cggcacttcc
    10621 gcaagtggcc tattcttggc atcagtggcc cggcaccaga aataaatccc atagcaacta
    10681 catacaatgc cgaactcgat actttaaaaa aatggatttc tatacgcctt caatggctcg
    10741 atgcaaatat tccgggatta tgtattccgg tgtttacttc aacaaatgag tccaataatt
    10801 ttagtcgctt caattttttt cctaatccta caaatggtat tgtacatttc gatggaggtg
    10861 tatttcaaaa ccgtgaagtg atttttcaaa tttatagttg tgatggaaaa ctgatcgagc
    10921 aaaagaattt tcttgatgga aaaataaaat tcgatttttt cttaaaggaa aaaggcgttt
    10981 tttattttac gtttactaat agtaatggta ttttacaacg cgggaagatt gttgttattt
    11041 gattagatca tcacatttca gaatcataaa ttatttttat ttgtgttaga ggctttgaaa
    11101 tggctaccca cactatttct agcattagag tctctcatac caaaacaaaa gaaaatgagg
    11161 actctaatgc gggtcatgct gctaaaataa ttattctatc acaattgttt ctcgatatag
    11221 aaaattgttg tcgtcaaaaa taatgatgtt ataaagtcct gcagcaattt tatccgtatc
    11281 gattgtcgtt ataccttctt ccagtattct tttcaaaatc aattgccctg ttgaattgta
    11341 aatcatcatt tgcaaatttt cggcattgtc catattatcg atatacaata tatcgtaagc
    11401 cggatttgga aacaacaaca ctttttgttc ttcgtaattt tcatcggtgt ttagaaatat
    11461 tttttcttcg ctaacaactg gttgaacagg gcatgcattt tttgcaggaa gttcaacttt
    11521 ccatatactg gcttcggtac tatcaccggt ttccattaca taaatcgaat tttctatttg
    11581 cacagcatca acaggatgcc aaaaatttgt gacgattttt ttagctgtaa actcataaac
    11641 attttttgtg ttgttttttg taaattctag ttggagtaaa tcttcgctat tatcgttgaa
    11701 tggcgtaagc agcgatgaag gttttggaag accagcattg ccacgtgtaa aactaagtac
    11761 aaagccatcg cccttcaagt cgcaaccaat gagcgaatcg gtatcgaaca atattcccaa
    11821 tggtgagcga tgtgatgtga gggaataaca tttttttcct aatgcactgg catcaattat
    11881 tgcaccggtt gcagtgtcgc gcatcatgtc ggcatcggga ccattgttgg cacagggaag
    11941 aataaatttt tgcgttggca ccggaaatgt tggatcattt ttccattgat tattaatgta
    12001 agagtaactt gtttttggaa ccatcacatt gtttgccgga ttgaagctgg aaaaatattg
    12061 cgggttccat gtgctgccaa gtacccacgg ataaccatag tgcttgcctt gccgtatcca
    12121 gtttatttca tcctcatgat cggcatcgct gctattttcg gcaccgaaca attcgccttt
    12181 tgcgttgtag gcaaaatcat aagtattgcg aattcctttt gctactaaac gcccttgcga
    12241 attaagtgtt gttacattat tgggaagaat aatattgata ctatctgcag gtactttcaa
    12301 aataattgct gctaatggca ctcctcgttt gttgggaaag gtaccatcaa acgaatctat
    12361 ttcaccatgg tcgccacgcg aaccgctgca caccactata gtatcctgag caggattaaa
    12421 tgtaatgccc gaaaaaagat gattaaaata ttttcccccg gcatatgatg ctgtagaagc
    12481 aactgtactc caggtatacg agccgttgtt ttgcagagta gctttcttta tatagctttt
    12541 gtaatatttg tttgcgagat tactacccga aacataaata gaattgttct taaccaataa
    12601 gccatacact acctgaatgc catgattact gccggtaaac agtaatgaat ctgtacggta
    12661 tggttgaaga aaatttttga taacgcgtat atcaccatca taagtagcgt aatataaatt
    12721 cgaatcgatt ttattataca ccattcgtgt gacgccttca tatggtatac ccattattct
    12781 tgtaatggta atgtcgtttc gcaatgatgt tggcgaactc ataggttgtt gcgcttttgc
    12841 ataaaaacaa aaagcaaaaa tggaaactaa agtgaggtaa gattttttaa gcatggtttt
    12901 gaaaaatttg gttagcacaa gagtataaat taatattatc actgacaaat atttttctat
    12961 agaaataatg ttgtgattat actgcgcacc agctttttat tcatcactaa aatatagaga
    13021 cgcacatttc aaaaaaacaa atgtgcatgg agtatcaatg atgattaaat tcaccaccag
    13081 caatactatt atacaccaag tactttattt tttttcaggg gctttaggtt cagtatgttc
    13141 aggcgtttat tttgtttcgg tagttttatc ttttgcgtcc tttttgtctc cctgttcatt
    13201 tgtttttttt gccacaggcc atacatcgtg ttctttgttc aaaatgcttt ctacacgtgt
    13261 aacaaaaagt ttttctttat cgatgccagg tacaccatat tcgccactat caaatttgta
    13321 gtaaaatatt tccggtttta attgttcgat tgtggagcgg taatttaccc acaactcctg
    13381 gtactgaaat gtttttaatg ccgaagtaag aatagtaaca gaagcagccg aaaccacaat
    13441 aaagtattgc atatcgtact cgccaattgt cttaactgca gcaagtaggg t
//
LOCUS       DAORQA010000035        12621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1346_length_12621_cov_16.020293, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000035 DAORQA010000000
VERSION     DAORQA010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1346_length_12621_cov_16.020293"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..645
                     /locus_tag="PK736_04045"
     CDS             130..645
                     /locus_tag="PK736_04045"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HRC32595.1"
                     /translation="MSNQTIFGIFNDEHKVLHAVEHLRSHNVNVTDVVSPYPIHGMDD
                     ALGLQKTRISICGFLFGITGTSLALLMIWYMNILDWPMDIGGKPNYAFFKNMPAFIPV
                     TFESTVLCAAHGMVITFLLRCKILPGVEPEVVHPRVTDDHYVMQIDTRASDDLEKIKA
                     MVMHEGAIEIR"
     gene            685..1326
                     /locus_tag="PK736_04050"
     CDS             685..1326
                     /locus_tag="PK736_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRC32596.1"
                     /translation="MMFLKNRNKIAIIGFVAVLTGLGSCRDAVKFPGYEYFPEMYRGP
                     AYETYAPSTIFADSLSSLKPVAGSVSRGFMPFNYSADNAGYEKASLEAFNPLRNDSIN
                     LGEGKRLYEIYCVNCHGAEGMGDGNLVADNKFPGVPPSYSTGTSSRGGTMKDLTDGKI
                     YHTITHGINLMGAHSGQLNPQQRWQITMYVRDLQKIGQATAAPAADSTAAAIK"
     gene            1365..2555
                     /locus_tag="PK736_04055"
     CDS             1365..2555
                     /locus_tag="PK736_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HRC32597.1"
                     /translation="MNFTFTGKHRTTFLVMILIGIISIISTFMLHPHQAWSNLLWNNF
                     FFMGIALVMVFFIAVQYVAEVGWSVVVLRIPEAMSQWLFVAMPIMIVIVIVGMMGGNH
                     LYHWMDDSLYVKGGEHYDKILAGKSGYLNKPFFLIRVLVYSAIWCGFAYLFRKQSLAE
                     ETSGDIALHKNGIKYGAIFLVLFAVTSSMMSWDFVMSIDSHWFSTMFGWYTFAGIWVS
                     GLTMIGLMTVYLKRKNLMAAVTSHHVHDIGKFMFAFSVFWTYLWVAQYMLIWYSNLPE
                     EIVYFRTRLDHYRWTFWIAFFLNFIIPFLVLMTRDAKRQFNILFFGGIVILCGHWLDS
                     FNMIIPGTLTAHGNHWQLSWMEVGTTIGFLGGFLYVTFRSLAKAPLLRTKHPLMMESV
                     QHAI"
     gene            2602..3627
                     /locus_tag="PK736_04060"
     CDS             2602..3627
                     /locus_tag="PK736_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRC32598.1"
                     /translation="MNFLILLGVIAAIMVAFRLMNILNISQELSGRDATEELHKDVKG
                     NAMGLIAFMVVGLILFFYMIWKYMPLTLPVAASEHGVRTDNLLNINFYIIIFVFIITQ
                     IALFYFIWKYQYRKGHMAYFYSHNNTVEIIWTVIPTIVLAILVITGLKEWIAITSKDN
                     TDGMNVQVYAQQFVFLGRYAGRDNTLGDSYFRNISTENPLGLKLEDKAVNDDIIAKTE
                     MHFPVNTPIMLNINSRDIIHSVYMPHFRVQMNAVPGMTTNFYFKPTITTAEMRKKTAN
                     EKFDYVILCNKICGVSHYTMNMKVVVDSKEDFVKWLKTQPLAKPAPTADNANASQTVK
                     EIAEAVK"
     gene            3687..5513
                     /locus_tag="PK736_04065"
     CDS             3687..5513
                     /locus_tag="PK736_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRC32599.1"
                     /translation="MESHVITHKHEDDHGHDHHHDDHHEHHQTFWTKYIFSTDHKMIS
                     KQFLVTAIVMAFLAMGMSMVFRLQLAWPDTKFAFLESILGKWGKDGKLDPGFYLALVT
                     IHGTIMVFFVLTGGLSGTFSNLLIPLQLGARDMASGFLNMLSYWFFFGSCTVMTISLF
                     VEGGPASAGWTIYPPLSALPTAISGSGAGMTLWLVSMVLFIVSALLGGINYVATIINL
                     RTEGMSMTRMPLTIWAFFITAILGILSFPVLFAAALLLVFDRSFGTSFYLSDIFIDGA
                     PLEHIGGSPILFQHLFWFLGHPEVYIVLLPSLGLASEVISVMSRKPIFGYKAMIGSMF
                     VIAFLSFIVWGHHMFMTGMNPFLGSVFVVTTLMVAIPSAVKVFNYIATLWRGNLILSP
                     AMLFAIGLVSFFISGGLTGIILGDSALDINIHDNYFVVAHFHIVMGASAIFGMFAGVY
                     HWFPRMFGRMMNFKLGYLHFWLTIICVYGVFFPMHFLGLAGVPRRYYANTAYAGFDSL
                     FEINQIVTVFAMIAGIAQVIFALNFFFSMFRGQRASQNPWNANTLEWTAPVEHLHGNW
                     PGAIPTVHRWPYDYGKPGHHEDFIPQTVPHSQTTSSNRPDDH"
     gene            complement(5783..6649)
                     /locus_tag="PK736_04070"
     CDS             complement(5783..6649)
                     /locus_tag="PK736_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC32600.1"
                     /translation="MTVTSTGAYTVTVTNTNGCTATSSTVTVTVLETPDATFLLDSTT
                     ICGALRIQFAIAKNPLPGASIKWNFGDGHTSTGPNPVHDFTKLDNVWVSLTIINQNGC
                     IDIDSVPIEIFFPQNPISNFTNNPEISTFLDPGIQFYDSCLNTTNWYWNFGDDEASFE
                     KNPFHYFKEAGEFTVTLVATNEIGCQDVHSETILITPFWIPNTFTPNADGKNDYFITS
                     NYIANVNSFHMTIFNRWGGQVFNSFSFDNPWDGIDLSGNAAQEGTYIYVIDINTKGGK
                     AHKFTGRIDLLR"
     gene            6667..8034
                     /locus_tag="PK736_04075"
                     /pseudo
     CDS             6667..8034
                     /locus_tag="PK736_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
     gene            complement(8304..12215)
                     /locus_tag="PK736_04080"
     CDS             complement(8304..12215)
                     /locus_tag="PK736_04080"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRC32601.1"
                     /translation="MISVKKCNTKWNTRFVIIILICIGKISNAQLVVTGGVGPITLVN
                     NILGNGVTISNVTYTGAVACRGSFTAAGTNLGMTSGICLSTGNAVQSGNAAVFAGEDK
                     NQPGDADIDAIVWPNITYDATILEFDFAAASDTAQFNYVFASEEYNEFVNSNFNDAFA
                     FLISGPGIVGQQNIALVPGVGIPVSINNVNNGFATGQSSGPCTNCTYFVDNDGGATIY
                     MDAFTTVLTATAPIQPCQTYHIKIVIADVGDGDYNSAVFIEGGSFTSAGQMQLYANGT
                     VANNNDTIYGCPGNIVNLSLSNLSSYVWSTGATTQNINVVVPSPGIVNQYIAYDSTLA
                     CSAVSTTIYVAPKTAVATITASGPTTLCNGGSVVLTANAGAGYLWSNGATTQSINVTT
                     TGSFTVTVTDALGCVATSAPLAIVINTTSSAINGPATFCNTTTANLIASAGSSWLWNT
                     GSTLQSLPVSTGGTYIVTVTNANGCTSSSSFSITALPSPSTTISGANAICQGTTAQFT
                     IGGIYNSILWSNGSTLSNISATAAGTYTVTVTNSNGCSGSASHQLIINPLPIPVINGA
                     ASICQGNSTILDAGIGYTSYLWNNNSNTQTISTNTGGTYTVTVTDNNGCSASSTLSVI
                     ILPLPVASISGNPIACADKNIILNAGAGFANYLWSNGNTTQQINPVQNGSYTVMVTDN
                     NNCSASASVNVIINPLPVPVINGNAEICKNDTSHLVVNGTFVSYLWSNSSSSSSISTI
                     MPGIYTVSVTDINGCSGSTSFAVAVNQLPTPHITGDTIVCYGNPANLETDSGYNQYIW
                     SNGNTGAAINTTMPGTYIVTVTDGKGCTNSVSANVIVNALPQPVIIGTPFICAGSVSV
                     LNAGSFSSYVWSNSATTQIQNTPLAGTYTVTVTNNNGCTNTTSFVVVVNPLPTPVITG
                     DDSICVGDFTILNAGNFVQFNWSNGSTTQPLNTMVQGMYIVTVTDANGCTASSAPFQV
                     EVMNPVATISTSGALVFCDGEDVTLSANIGNNYLWSNGSTSQTVTVTSTGAYTVTVTN
                     TNGCTATSSTVTVTVLETPDATFLLDSTTICGALRIQFAIAKNPLPGASIKWNFGDGH
                     TSTGPNPVHDFTKLDNVWVSLTIINQNGCIDIDSVPIEIFFPQNPISNFTNNPEISTF
                     LDPGIQFYDSCLNTTNWYWNFGDDEASFEKNPFHYFKEAGEFTVTLVATNEIGCQDVH
                     SETILITPFWIPNTFTPNADGKNDYFITSNYIANVNSFHMTIFNRWGGQVFNSFSFDN
                     PWDGIDLSGNAAQEGTYIYVIDINTKGGKAHKFTGRIDLLR"
BASE COUNT         3638 a         2344 c         2543 g         4096 t
ORIGIN      
        1 tcggctcact ggggttattt tttgtaccat ttttattatt cacaagattt tttccagtaa
       61 tagctatgag cgaattgaaa accatttttt atacatcaac atcacaagta aagaagggag
      121 gggcccatca tgagtaacca aacgattttc ggaattttta acgatgagca taaagtgctc
      181 catgcagtcg agcatcttag atcgcataat gtaaatgtta ccgatgttgt atcaccatat
      241 ccaattcatg gtatggatga tgcccttggt ttgcaaaaga ctagaatatc tatttgtgga
      301 tttctatttg gcattacagg tacaagttta gctttgttga tgatatggta tatgaatatc
      361 ctggattggc caatggatat tggagggaaa cccaattatg catttttcaa aaatatgccg
      421 gcctttattc cagtaacatt tgaatcgact gtattgtgcg ctgcccatgg catggtgatt
      481 acgtttttac ttcgttgcaa aatattaccc ggtgtagaac ctgaagtggt acatcccagg
      541 gtaactgacg accattatgt gatgcaaatt gatactcgcg cgagtgacga tttagaaaaa
      601 ataaaggcca tggtgatgca cgaaggcgcc attgaaataa gataaaacag atacttcatt
      661 catattaatg ataatagcat ataaatgatg tttttaaaaa atagaaataa aatagcgata
      721 attggcttcg ttgcagtatt gaccggatta ggttcgtgcc gcgatgcagt aaaatttccc
      781 ggttatgaat actttcccga aatgtatcgt ggaccggcat atgaaaccta tgctcccagt
      841 actattttcg cagatagtct gtcatcctta aagcctgttg cagggtcggt ctcacgcggc
      901 ttcatgccat ttaattattc tgctgataat gcaggatatg aaaaagcaag ccttgaagct
      961 tttaacccgt tacgcaatga ttctataaat ttgggagaag gcaaaaggtt atacgaaata
     1021 tattgtgtca attgccatgg tgcagaaggt atgggcgatg gtaacttagt tgctgataat
     1081 aaatttcccg gagtgccgcc atcatattct acaggaacct catcaagagg aggtactatg
     1141 aaagacttaa ccgatggaaa aatataccac accatcaccc atggaattaa ccttatgggc
     1201 gcacattcgg ggcaattaaa tccacaacaa cgttggcaga ttacaatgta tgtgcgcgac
     1261 cttcaaaaaa tagggcaagc cactgcggca ccggcagccg attcaactgc ggcagctata
     1321 aaataaatat tattaaaaat ttgacttaca gaataaatta gaatatgaac ttcacattta
     1381 caggtaaaca tcgcactact ttcttagtga tgattttgat cggaatcatt tctatcattt
     1441 ccacttttat gcttcacccg catcaggcat ggagtaattt attatggaac aatttcttct
     1501 ttatgggaat tgctcttgta atggtgtttt ttattgcagt acaatatgtt gccgaagtag
     1561 gctggagtgt agtcgtactg cgaattccag aagccatgag tcagtggttg tttgttgcta
     1621 tgcctattat gattgtaatt gttatagttg gtatgatggg aggaaatcac ttatatcatt
     1681 ggatggacga ttcattgtat gtaaaaggag gagaacatta cgataaaatt cttgctggaa
     1741 aatcagggta tttgaacaag ccatttttct tgatacgtgt tttagtttac tcagccattt
     1801 ggtgcggttt tgcatattta tttcgtaagc aatcgcttgc tgaagaaact tcaggcgata
     1861 ttgctttaca taaaaacggc ataaaatatg gggctatttt tcttgtttta tttgcggtta
     1921 cttcttctat gatgtcttgg gattttgtga tgtcaatcga ttcacattgg ttctcaacta
     1981 tgtttggctg gtatacattt gccggtattt gggtaagcgg attaacaatg attggtttaa
     2041 tgactgttta cttaaagcgt aaaaacttaa tggctgcggt tacttcacat cacgtgcacg
     2101 atattggaaa atttatgttt gcattttctg tgttttggac ttacttgtgg gtagctcaat
     2161 acatgttgat ttggtattca aatttgcctg aggagattgt ttatttccga actagattag
     2221 accattaccg ttggactttc tggatagcct ttttcctgaa ctttatcatt ccatttttgg
     2281 tattaatgac acgcgatgcc aagcgacaat ttaatatatt gttttttggt ggtatagtta
     2341 ttctttgtgg gcattggctt gattcattta acatgataat tcccggaacg ttaacagctc
     2401 atggaaatca ttggcaatta tcatggatgg aagttggaac aacaatagga tttttgggtg
     2461 gattcttata cgtaacattc cgttctttgg caaaagcacc tctgttacgt accaaacatc
     2521 cgcttatgat ggaaagcgtg cagcacgcaa tttaattttg taataaatcg attaacacat
     2581 ctgtaaaata aaagtaacga aatgaatttt ttaattttat taggagtaat agcggcaata
     2641 atggtagcct tcagactgat gaatatttta aatatcagtc aggaattgtc gggtagagat
     2701 gctaccgaag aattgcacaa agatgtaaaa ggcaatgcta tgggcttgat agcctttatg
     2761 gttgttggct tgattttgtt tttctatatg atatggaaat atatgcctct taccctccct
     2821 gtggccgcat cggagcatgg agtaagaaca gataacttat tgaatataaa tttttatatc
     2881 atcatttttg ttttcattat tacacaaata gcgttatttt attttatttg gaaataccaa
     2941 taccgtaaag gtcatatggc atatttctat tctcataata atacagtaga aattatttgg
     3001 acagttatac ctactatcgt tttagccatt ttagttataa ccggcttgaa agaatggatt
     3061 gcaataacaa gtaaagacaa taccgatggt atgaatgttc aggtttatgc acagcaattc
     3121 gtatttttag gacgctatgc aggccgagat aatactttgg gcgattcata tttccgcaac
     3181 atttctactg agaatccatt agggttaaaa cttgaagaca aagcagtaaa tgatgatatt
     3241 attgctaaaa cagagatgca ctttcctgtg aacacaccaa taatgttaaa tataaactcg
     3301 cgtgatataa ttcactctgt ctatatgcca cactttcggg tacaaatgaa tgcagtgcct
     3361 ggaatgacaa ccaattttta tttcaagcca actattacta ctgccgaaat gcgcaaaaaa
     3421 accgccaacg aaaaatttga ttacgtaatt ctatgtaaca aaatttgtgg cgtgtcacat
     3481 tatactatga atatgaaagt ggtggttgac agcaaagaag attttgttaa atggttgaaa
     3541 acacaacctt tggcaaaacc tgcacccacg gccgataatg ctaatgcttc acaaacggtt
     3601 aaagaaattg ccgaagcagt aaagtaattt tcatttgaat tatctaaact cattttgttt
     3661 tgtttaaaat ataaaagatt tataatatgg agtcgcacgt aataacacat aaacatgagg
     3721 atgaccacgg tcatgatcac catcatgatg atcatcatga acatcatcaa acattttgga
     3781 cgaagtatat tttcagcacc gatcacaaaa tgatttcgaa gcagtttttg gtaactgcaa
     3841 tagtgatggc ttttttggca atgggcatgt ccatggtctt tcgtttacaa cttgcctggc
     3901 ccgatacaaa atttgctttc ctcgaatcga ttttgggcaa gtggggaaaa gacggcaaac
     3961 ttgatcctgg tttttatctg gcgttagtta ccatacatgg tactatcatg gtgttctttg
     4021 ttttaactgg tggtttaagc ggaaccttta gtaacttact cattcctttg caattaggtg
     4081 cacgcgatat ggcttcgggt ttcctcaata tgttatcgta ttggtttttc ttcggctcgt
     4141 gcactgtaat gacaatttcg ttgtttgtag aaggtggacc tgcatcggca ggatggacaa
     4201 tatatcctcc tttgagcgcc ttgcctacag ctatttccgg ctccggtgca ggtatgacct
     4261 tgtggcttgt aagtatggtt ttatttattg tttcggctct cctgggcggc ataaattatg
     4321 tggcaaccat aataaacctt cgtaccgaag gaatgagtat gacccgcatg ccactaacca
     4381 tttgggcgtt ttttataact gctatattag gaatattatc tttccctgtt ttgtttgccg
     4441 cagctttatt gctcgtattt gaccgttcat ttggaacaag tttttatctt tcagatattt
     4501 ttatcgatgg agctccatta gaacatattg gaggttcacc aatcttattc caacatttat
     4561 tttggttcct tggtcacccc gaagtttaca ttgtattgtt gccttcactt ggattagcat
     4621 cggaagtaat atctgtaatg agccgtaagc caatatttgg ttacaaggcc atgattggct
     4681 ctatgttcgt gattgcattt ttatcgttta tagtttgggg tcatcatatg tttatgacag
     4741 gaatgaatcc attcttaggt tcggtgtttg tggtaaccac acttatggtt gccataccgt
     4801 ccgcagttaa agtgtttaac tacattgcta ccttgtggcg aggcaatctt atattatcgc
     4861 ctgccatgtt gtttgcaatt ggtttggttt cgttctttat ttcaggagga cttacaggaa
     4921 ttattttggg agactccgct ttggacataa acatacacga taactatttt gtggtagcac
     4981 attttcatat tgtaatgggc gcatctgcaa tttttggaat gtttgccggt gtgtatcatt
     5041 ggtttccccg catgtttggc cgtatgatga atttcaaact tggttattta catttttggc
     5101 ttacaataat ttgtgtgtat ggcgtgtttt tccctatgca ctttttagga ttagccggtg
     5161 taccaagaag gtattatgcc aacacagctt acgcaggttt tgacagcctt tttgaaataa
     5221 atcaaatagt aactgtgttt gcaatgatag caggaattgc acaagtaata tttgcactca
     5281 atttcttctt ctctatgttt agaggacaac gtgcctcgca aaacccttgg aatgccaaca
     5341 cgcttgagtg gacagcacct gtggagcatt tacatggtaa ctggcccgga gcaataccca
     5401 cagtacaccg ttggccgtac gattatggta agcccggaca tcatgaggat tttataccac
     5461 aaacagtacc tcactcgcaa acaacatcga gcaaccgccc cgatgatcat taatagataa
     5521 aaagaaaatc cctcaccatg agggattttt tttggatgat actatttcta agcaacgtat
     5581 atggtgaggg ttacaatcta tggctgaaat aaatttctgg aatttcatat gacgtttgct
     5641 caataattga attccgaagt aaccctaatg cttataagca atggaaaata ctactgataa
     5701 tcgaataaag gagggttcgt aataagtttg tccgcttttc aaatttaggg ttacacaact
     5761 atcgaaaaag gttaattgtt ttttatcgca gtaaatctat cctgcctgta aacttatgtg
     5821 ccttgccccc tttggtattg atgtcaataa cataaatata ggtgccttct tgtgctgcgt
     5881 tgcccgataa atctatacca tcccatggat tgtcgaatga gaaactgttg aaaacctggc
     5941 cgccccaacg gttaaaaatg gtcatatgga atgagtttac atttgcaata taattgcttg
     6001 ttataaaata atcattcttg ccatcggcat ttggtgtaaa tgtatttgga atccaaaaag
     6061 gcgtaatcag tattgtttcg gaatgaacat cttgacaacc tatctcgtta gtagcaacca
     6121 atgttactgt aaactcgccc gcctctttaa agtagtggaa aggatttttt tcgaaagacg
     6181 cttcatcatc gccaaaattc cagtaccaat ttgtggtgtt taagcaagaa tcataaaact
     6241 gtattccagg gtctaagaat gtgcttattt ctggattgtt tgtaaagtta gaaatagggt
     6301 tttgtggaaa aaatatttcg ataggaacac tatctatatc tatacaccca ttttgattaa
     6361 ttatggttaa tgaaacccac acgttgtcaa gcttcgtgaa atcatgcacc gggtttggcc
     6421 ccgtgctggt gtggccatcg ccaaagttcc attttattga agctccgggc aatgggtttt
     6481 ttgctatagc aaattgtatt cgcaaggcac cgcatatggt tgtactgtct agtaaaaaag
     6541 tagcatcggg cgtttcaagt actgtaacgg ttactgtaga tgatgttgct gtgcaaccat
     6601 ttgtattagt aactgtaaca gtatacgctc ccgtacttgt aactgtcact gtttggctcg
     6661 tgcactgtaa tgacaatttc gttgtttgta gaaggtggac ctgcatcggc aggatggaca
     6721 atatatcctc ctttgagcgc cttgcctaca gctatttccg gctccggtgc aggtatgacc
     6781 ttgtggcttg taagtatggt tttatttatt gtttcggctc tcctgggcgg cataaattat
     6841 gtggcaacca taataaacct tcgtaccgaa ggaatgagta tgacccgcat gccactaacc
     6901 atttgggcgt tttttataac tgctatatta ggaatattat ctttccctgt tttgtttgcc
     6961 gcagctttat tgctcgtatt tgaccgttca tttggaacaa gtttttatct ttcagatatt
     7021 tttatcgatg gagctccatt agaacatatt ggaggttcac caatcttatt ccaacattta
     7081 ttttggttcc ttggtcaccc cgaagtttac attgtattgt tgccttcact tggattagca
     7141 tcggaagtaa tatctgtaat gagccgtaag ccaatatttg gttacaaggc catgattggc
     7201 tctatgttcg tgattgcatt tttatcgttt atagtttggg gtcatcatat gtttatgaca
     7261 ggaatgaatc cattcttagg ttcggtgttt gtggtaacca cacttatggt tgccataccg
     7321 tccgcagtta aagtgtttaa ctacattgct accttgtggc gaggcaatct tatattatcg
     7381 cctgccatgt tgtttgcaat tggtttggtt tcgttcttta tttcaggagg acttacagga
     7441 attattttgg gagactccgc tttggacata aacatacacg ataactattt tgtggtagca
     7501 cattttcata ttgtaatggg cgcatctgca atttttggaa tgtttgccgg tgtgtatcat
     7561 tggtttcccc gcatgtttgg ccgtatgatg aatttcaaac ttggttattt acatttttgg
     7621 cttacaataa tttgtgtgta tggcgtgttt ttccctatgc actttttagg attagccggt
     7681 gtaccaagaa ggtattatgc caacacagct tacgcaggtt ttgacagcct ttttgaaata
     7741 aatcaaatag taactgtgtt tgcaatgata gcaggaattg cacaagtaat atttgcactc
     7801 aatttcttct tctctatgtt tagaggacaa cgtgcctcgc aaaacccttg gaatgccaac
     7861 acgcttgagt ggacagcacc tgtggagcat ttacatggta actggcccgg agcaataccc
     7921 acagtacacc gttggccgta cgattatggt aagcccggac atcatgagga ttttatacca
     7981 caaacagtac ctcactcgca aacaacatcg agcaaccgcc ccgatgatca ttaatagata
     8041 aaaagaaaat ccctcaccat gagggatttt ttttggatga tactatttct aagcaacgta
     8101 tatggtgagg gttacaatct atggctgaaa taaatttctg gaatttcata tgacgtttgc
     8161 tcaataattg aattccgaag taaccctaat gcttataagc aatggaaaat actactgata
     8221 atcgaataaa ggagggttcg taataagttt gtccgctttt caaatttagg gttacacaac
     8281 tatcgaaaaa ggttaattgt tttttatcgc agtaaatcta tcctgcctgt aaacttatgt
     8341 gccttgcccc ctttggtatt gatgtcaata acataaatat aggtgccttc ttgtgctgcg
     8401 ttgcccgata aatctatacc atcccatgga ttgtcgaatg agaaactgtt gaaaacctgg
     8461 ccgccccaac ggttaaaaat ggtcatatgg aatgagttta catttgcaat ataattgctt
     8521 gttataaaat aatcattctt gccatcggca tttggtgtaa atgtatttgg aatccaaaaa
     8581 ggcgtaatca gtattgtttc ggaatgaaca tcttgacaac ctatctcgtt agtagcaacc
     8641 aatgttactg taaactcgcc cgcctcttta aagtagtgga aaggattttt ttcgaaagac
     8701 gcttcatcat cgccaaaatt ccagtaccaa tttgtggtgt ttaagcaaga atcataaaac
     8761 tgtattccag ggtctaagaa tgtgcttatt tctggattgt ttgtaaagtt agaaataggg
     8821 ttttgtggaa aaaatatttc gataggaaca ctatctatat ctatacaccc attttgatta
     8881 attatggtta atgaaaccca cacgttgtca agcttcgtga aatcatgcac cgggtttggc
     8941 cccgtgctgg tgtggccatc gccaaagttc cattttattg aagctccggg caatgggttt
     9001 tttgctatag caaattgtat tcgcaaggca ccgcatatgg ttgtactgtc tagtaaaaaa
     9061 gtagcatcgg gcgtttcaag tactgtaacg gttactgtag atgatgttgc tgtgcaacca
     9121 tttgtattag taactgtaac agtatacgct cccgtacttg taactgtcac tgtttggctc
     9181 gtgctgccat tcgaccatag ataattgttc ccgatattag ctgatagggt gacatcttcg
     9241 ccatcgcaaa aaaccaaagc accacttgta gaaattgttg caacaggatt catcacttct
     9301 acctgaaatg gagcagaact tgccgtacat ccatttgcat cagtaacagt tacaatatac
     9361 ataccctgaa ccattgtgtt gagtggttga gtagtgctgc catttgacca attaaactga
     9421 acaaaatttc ctgcattcaa aatagtaaag tcaccaacac agatgctatc gtctcctgtt
     9481 attacaggag taggtaaagg gtttacaaca actacaaatg atgtagtatt tgtgcatcca
     9541 ttattgtttg taacagtaac ggtataggtg ccggcaagtg gtgtattttg aatttgggtg
     9601 gtagcactgt ttgaccatac atagcttgaa aaactccctg catttaacac cgatacacta
     9661 cccgcacaaa taaatggagt gccaattatt acaggctgtg gcaaggcatt aacaattaca
     9721 tttgccgaaa cagaatttgt acaacccttg ccatcggtaa cagtaactat atacgtgcca
     9781 ggcatggttg tgtttatggc cgcgcctgta ttgccattgc tccaaatata ctgattataa
     9841 ccactatcag tttcaagatt tgctggattg ccgtaacaaa caatggtatc gccagtaatg
     9901 tgtggtgttg gaagttggtt taccgctaca gcaaatgaag ttgagccgct gcaaccattt
     9961 atatcggtga cagatactgt atatatgccc ggcataattg tgctgataga tgaactgcta
    10021 ctactgtttg accatagata gcttacaaat gttccattaa caactaaatg actggtgtcg
    10081 tttttgcata tttcggcatt gccatttatt acaggtaccg gcaatggatt tattattacg
    10141 tttaccgatg ccgaagcaga gcaattattg ttatcggtaa ccatcactgt ataactacca
    10201 ttttgaaccg ggtttatttg ctgagttgta tttccattgg accataagta attagcaaac
    10261 cctgctccgg cattcaatat aatattttta tcggcacatg ctatcggatt gcctgatata
    10321 cttgcaacag gtaaaggcag aatgataacc gacaaagttg atgaggcact acaaccatta
    10381 ttatctgtaa ctgtaacagt atacgtgcct cctgtatttg tgcttatagt ttgagtatta
    10441 ctattgttgt tccacaggta tgatgtgtat ccaatacctg catccagtat ggtactatta
    10501 ccctgacaaa ttgatgcagc tccgtttatt acaggtattg gaaggggatt tattatcaat
    10561 tgatgactgg cactgccgct gcatccatta ctattggtaa ccgttactgt atatgtgccc
    10621 gctgcagtcg cacttatatt tgatagtgtt gaaccgttgc tccataaaat ggaattatag
    10681 atgcctccaa tagtaaactg tgccgtggtt ccctggcaaa ttgcgttagc tccgctgatt
    10741 gttgtagaag gagaaggtaa agctgtaatg ctaaaacttg acgatgatgt acaaccattt
    10801 gcatttgtaa ctgtaacaat atacgttcca cctgttgaaa caggcaatga ttgaagtgta
    10861 gagcccgtat tccataacca tgagctacct gcacttgcaa ttaaatttgc ggtagttgta
    10921 ttgcaaaaag ttgcaggccc attgattgca gagctggtag tattaattac aatagccaat
    10981 ggtgcactgg tagcaacaca tcccaacgca tcagttacag ttacagtaaa tgaaccggtt
    11041 gttgtcacat taatactttg agtggtggca ccattgctcc ataaatagcc ggctccggca
    11101 tttgcggtaa gtacaacact acctccatta catagcgtag tgggtccact tgcagtaata
    11161 gttgctactg ccgttttagg agcaacatat atggtagttg atacagctga gcatgcgaga
    11221 gtagagtcgt atgctatata ttggttcact ataccaggtg atggcactac aacatttatg
    11281 ttttgagttg ttgctcccgt actccataca taactcgata aattgctcaa actcagattg
    11341 acaatatttc ccggacaacc atatatagta tcgttgttgt ttgcaacagt gccatttgca
    11401 taaagttgca tttgtccggc actggtaaaa cttccaccct ctatgaaaac tgcgctgtta
    11461 taatccccat caccaacgtc tgcaattaca attttgatat gatatgtttg acatggctga
    11521 ataggagcag tagccgttaa cacagtagta aaagcatcca tataaatggt agcgcctcca
    11581 tcgttatcta caaaatatgt acagttggtg caagggccgc tactttgccc ggttgcaaaa
    11641 ccattgttta cattgttgat agaaacagga attcccaccc cgggaaccaa tgcaatattt
    11701 tgctgtccaa ctatgcccgg gccgctaatt aaaaaagcaa aagcatcgtt gaaattggag
    11761 tttacaaatt cattgtattc ttccgaagca aatacataat taaattgtgc tgtatcggag
    11821 gccgctgcaa aatcgaattc aagaatagta gcatcgtatg taatatttgg ccatacaatt
    11881 gcgtcaatgt ctgcatcacc gggttgattt ttatcttctc cggcaaacac tgcagcattt
    11941 ccactttgaa ctgcgttgcc tgtactcagg catatacccg aggtcattcc cagatttgta
    12001 cctgcagcag taaatgatcc ccggcaagcc accgctcctg tataagtaac attgcttatt
    12061 gtaacaccat tgccaagaat attgtttacc agggttatag ggcctacacc accagtaaca
    12121 acaagttgtg cattacttat cttacctatg caaataagaa taataatcac aaatcttgtg
    12181 ttccactttg tattgcactt ttttacacta atcattttaa attgaagtga ggtttttcaa
    12241 tattttattt actaccaaaa tatcatcaat gggtattgaa tcgatattat tacatgtggt
    12301 aatatttgaa gcaattatag ccaaaatagt tagtatttga aaataaaaat tgctcaagcg
    12361 gaatatttaa gattcaaacc ggaatcaaca gattaaatat tttatgatta aagcatttaa
    12421 ggcctaagcg cacaatgtga aaaaaggcac catcgaatta ttttatattc aaccaatgac
    12481 aaggaatttg gggattgaaa attaggaaat tgggagttga aaattaagga ttaaaaatga
    12541 ccaataacca accaccaatg acgagtatgc cgacactttg gtcggcatcc cgaccgaagt
    12601 gtcggggttt aaaatgacca a
//
LOCUS       DAORQA010000036        12076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1425_length_12076_cov_12.365028, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000036 DAORQA010000000
VERSION     DAORQA010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12076
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1425_length_12076_cov_12.365028"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..1954
                     /locus_tag="PK736_04085"
     CDS             293..1954
                     /locus_tag="PK736_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRC32602.1"
                     /translation="MRKLFCSVVMVFTFIYAATAQQILTCKATVDKATVYLTGATLTS
                     TADFNLKQGINELVFENISSGLNTQSLQASGKGNFVIMDVRCNVKMNEQPTVKPDTKF
                     DKIINQLNDSITEMEFVIEELTERNTALQSEKNILLNNRLMKGETKQDTLALFTEAIS
                     FLRSRLENITAETFKVKRALQKNNVALANLQQRYQELIDAKNKSAIPKISTASVCQAI
                     VTVMAETESASRVSVSYYVNGAGWKPTYDIRASGTSNTIGITHKAQLYQNTGLDWNNT
                     AITLATATPNQGINKPQLNPQLIGYFMAVMREKRKALNKQELDEVAVGTTTMDAKKPE
                     MKDDAEDMALYTDMVENLVQTEYNIKLKYNIPADGKEHMVVIQNTEVKADYNFSAVPK
                     LDANAYLQARITDWSDLNLLPGISRIFFDNSFVGQSVIDPYITNDTMLLSLGKDKSII
                     MQRKKLKDKTRQKFLGEDKTISYSFEISLKNTKSLPVNITVEDQIPISNTKEIEVKLI
                     AGNKANYNVETGKLEWDLQLKPGESKKLLVTYEVKLPKNKTVAGM"
     gene            2334..2843
                     /locus_tag="PK736_04090"
     CDS             2334..2843
                     /locus_tag="PK736_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRC32603.1"
                     /translation="MQKFETTILKFGSMGEKTGWTYIIIPADVAQQVKPGNKVSFKIK
                     GKLDNYKFSGLSVLPMGKGDFILALNAEIRKAIGKRHGAMLMVQLEEDKSPFIMNADF
                     MECLNDAPDAVEFFETLSGSHQRYFSKWVDSAKTIETKTKRIALAINALSKKLGYPEM
                     LRSQKQNKL"
     gene            complement(3115..4203)
                     /locus_tag="PK736_04095"
     CDS             complement(3115..4203)
                     /locus_tag="PK736_04095"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRC32604.1"
                     /translation="MDRVKASVNTSKSGLAGSKSRLEQAKSQSEKADFAFKRAKQLYD
                     DKVISQQDFENAKSAYDIAKAEVDAALQNIKASEFGIQGANASLKQAQENLNKTILYA
                     PVDGTISSLSKEKGERVVGTNMMDGTEIMRLANLNEMEVIVDVSETDIIRVSMSDTAD
                     IEIDAYDDRKFKGIVTEIANAAKTSLMQQSTDQVTNYTVKVRILRDSYKDLMNESNAN
                     QSPFRPGMSASVEIRTKEASNVVAVPIQCVTTRDTSKKADAKKIKIAIGGENKEEEKK
                     DDKAPSKDDEANIKECVFVIEDGKAKRILVSTGIQDNNFIEIKTGLKGGEKIITGPYL
                     TISKLINESDKIKVVSKDELFEPDLEKK"
     gene            complement(4213..4506)
                     /locus_tag="PK736_04100"
     CDS             complement(4213..4506)
                     /locus_tag="PK736_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRC32605.1"
                     /translation="MLRNKWFIIGLIAVILLIALVVAKKNGLIGESGDKKVSMELVSR
                     HNIVEIVTASGKVQPEAELKITSDVSGEIVDLFVKEGQVVKKGDLLVKIRPDI"
     gene            complement(4538..5908)
                     /locus_tag="PK736_04105"
     CDS             complement(4538..5908)
                     /locus_tag="PK736_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRC32606.1"
                     /translation="MEAQQRWSLKECVEYAMNNNLMVQQSALAGQGTHVTLIQNKAAL
                     LPSVNGSASQNINFGRSVDPYTYQFTNQQISNTQLSLSANVVLFDGFQLMNTVKQSKL
                     DYQAGKHDLEKVINDISLSVASSYLQVLFSKEQLKAAKLRIESIEKQRDVIKKQVLAG
                     VLAEGSLMDLEAQVASEELQQINASNSVKQSLLTLVQIMNLDSAENFDIEDPNLELAD
                     QSIVGFTAYQIYENALRAMPEVKAAEIRSQSAGTALSIARGGYSPRLTAFGSVSSGYS
                     SGAKRLAGTPQFTGFAPNGQITSAFDTVLSPSFKTVFEETPFNTQVNENLNKGIGLSL
                     QIPIFNAWSTHSNISRAKINMARTNVDLQNTKQQIFKTIQQAHLDAKSAQLKYAAAQK
                     AYSAALLSSGYSEKKFNAGMGTSLEYLTAKNNATRAQSDLLQAKFDLIFRIKVLEFYN
                     GQKLTL"
     gene            6078..7028
                     /locus_tag="PK736_04110"
     CDS             6078..7028
                     /locus_tag="PK736_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32607.1"
                     /translation="MSKWIETLAAKWKLDPGEKSNIETYESKKLFSIYYELQTLITVG
                     VLLLATSAGIFLYKNIDTIGHSILVLLTGIAAVSCFAFCIFKKEKQANITYYDNVLYL
                     GCMLLVSCVGYWQYQYGIFGKHHAFAILVPAIIITAFAYYFNDRAILSLAITLWAGYL
                     GFTISIPAILFGQNFRSLQLIFTAIIFACVLMALAYAHYHYKKNLPFGFTIASFAFHI
                     MCLALLAGIFNQPYSWIWAATLVVICFAAVLFSLVEQSYATFVSAVLYTYIAVSYLCI
                     NFLMHHHILESTYFLLIYFIATSIMLIKYIRVGKQKLFKA"
     gene            7028..>8190
                     /locus_tag="PK736_04115"
     CDS             7028..>8190
                     /locus_tag="PK736_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32608.1"
                     /translation="MIYNIEKLRNKLLLPIATEWMAENVMDKKNIPLLTQVQKDAYKP
                     SQIAFQVGLFLLTFILAFCSAGLIAMFFGFTNSSMAFGMICCFFSILSFVAAYYFINT
                     KLHFKTGVDTALYYFSIISLMLGIGLMLDNLWNNTSTLVAFCMLCSGLLLLYAFLFFD
                     FVLAMIAIMVFATGYANAFYETTAIIKLCASLLFPFVCFAFYKTILYFENKMDVVYQN
                     IFPYCKFMLAVVAYIAINRSFVNEVFFEYTLKYLNKNVFAAFSIFSVAWSWLLPLVYI
                     FYGLYKNERSFLIAGITCMALFICSIPYFHPSAIPEVMMLIGSVSCFIFVGLSNYFFK
                     NNAWKITLQKSKYDSAILSKEMQGIIMSQAIATKTISQDTTEFGGGKFGGGGG"
     assembly_gap    8191..8213
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8443..9708)
                     /locus_tag="PK736_04120"
     CDS             complement(8443..9708)
                     /locus_tag="PK736_04120"
                     /inference="COORDINATES: protein motif:HMM:NF025639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRC32609.1"
                     /translation="MRENIYTLDQIRDEIIKFKNDSDFQKLESFYYSKSFSEILGVSR
                     REISHSGFLAWLFNNLESHNLGEFAIRKFVDIITTFSGDKLKNKHNALFNFLITENYS
                     IERISVHPEYAIKNVGRLDIYIELALSISDQKRNIKFVIENKVGSKENNDQTTNYYHH
                     FNPLETEDNIIFYIYLTPISTLELIELTEPESNCKEFIQINYQSLVDYLIEPALNQNI
                     SARTQNILTEYLKSLSQPSIEEAADGHKQELIMALGNEERKLLSSFWEKNKKLILASL
                     YAISSDPEQEKDTRDSITEALDNLSSDKDRSTFNIKYKEQFFTSNIIKAEIGLQTLKL
                     FEKYNLINDQNIEYLRQDKSCSFQLLKKQEELSEYDIKYKKYKTTKESEIVYNNESYF
                     VARNWGINNIDKFINKFKEKFPGLEYEAN"
     gene            complement(9889..10872)
                     /locus_tag="PK736_04125"
     CDS             complement(9889..10872)
                     /locus_tag="PK736_04125"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HRC32610.1"
                     /translation="MKNLVADFSAHLGAAIEIGQNAAIAKPEIEIRNVLITGLGGSGI
                     GGTIAAQCLDQELAVPVVVCKDYFPPAFINQHTLVIVCSYSGNTEETVQAFEVALKKG
                     AQIVCVASGGKIIDLARTNGIENIIIPGGMPPRAMFGYSFTQLFFVLNKKGLCSDKFL
                     DEINDAIKMLQANEEATQAEAMEVAKKVMGKIAVLYSDAGYEGVCVRFRQQINENSKM
                     LCWHHALPEMNHNELVGWVEKNENLAVIFMRNNDDYIRTQTRLDYTKEVASNVTSTII
                     EVWSKGESKLARTLYLVHIGDWISCYLADMKGIDAVEVNVITKLKNALAGK"
     gene            complement(11039..11863)
                     /locus_tag="PK736_04130"
     CDS             complement(11039..11863)
                     /locus_tag="PK736_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRC32611.1"
                     /translation="MMKKTKVLFVTQEISPYLDETELSKICRYLPQGTQESGKEIRVF
                     MPKYGTINERRNQLHEVIRLSGMNLIIDDTDHPLIIKVASIQSARMQVYFIDNEEYFQ
                     RKATVTDAKGKHFKDNDDRTIFYCRGVLETVKKLGWQPDVIHCHGWMTSLIPLFIKTS
                     YKEDPHFKNAKVVYSLYNDSFEDTFNPEYSKKLMMEGIAASDVKELKDPTYSNIVLNA
                     MSKSDAFIQMGEDMPKEYAAHIKKSGKPCLKHPEENYIKAYNEFYELIAEEETIEA"
BASE COUNT         3540 a         2234 c         2167 g         4112 t
ORIGIN      
        1 ctaaagccaa tacaaattat taagaccgtc cgataaatcg gacggcaatg atttgctgcc
       61 gcgaaaaact ttgtgcttta gtgtctttgt ggcaaaaaag tatttggcat ccgttctggc
      121 atgggttgct aaaaagcatc tgttccaaat tcctccgtgt tctcagtgcc tccgtgcgaa
      181 atgcatttat attctcacaa aaaaaatatt tccctctccc gtatggaata aacttttgca
      241 tttcatcctc atactgaaaa tcaattataa aatttactaa aataaaaatc gtatgagaaa
      301 attattttgc tccgtagtga tggtattcac ttttatttat gctgcaacag cacaacagat
      361 tctaacatgt aaagccacgg ttgacaaagc cactgtatac cttacaggcg ctacactcac
      421 aagcactgcc gacttcaatt tgaaacaagg cataaacgaa cttgtattcg aaaatatttc
      481 ttcagggctt aacacacaat cgttgcaggc atcgggcaaa ggaaactttg taataatgga
      541 tgtgaggtgc aatgtaaaaa tgaatgagca accaaccgta aagcccgata caaaatttga
      601 taaaataata aatcagctca acgactctat taccgaaatg gaatttgtaa tagaagaatt
      661 aactgaacgc aacaccgcat tgcaaagcga aaaaaatatt ttgctcaaca acagattaat
      721 gaaaggcgaa acaaaacaag acacgcttgc actattcacc gaagcaattt cgtttttacg
      781 ttcgcgatta gaaaacatta ctgccgaaac ttttaaagta aaacgtgcat tgcaaaaaaa
      841 caatgttgct cttgcaaatt tgcaacagcg ttatcaggaa ctgattgacg ctaaaaacaa
      901 aagcgctatt ccaaaaatat caactgcatc ggtatgccag gcaatagtaa ctgtaatggc
      961 cgaaacagaa tctgcctcaa gagtatcggt aagctattat gtaaatggtg caggctggaa
     1021 accaacttat gatatacgtg catcgggaac cagcaatact attggcataa cacacaaagc
     1081 acaattgtac caaaacaccg gtctcgactg gaacaatact gccattacac tcgctactgc
     1141 aacacctaat caaggcataa acaagccaca acttaatcca caacttatag gttactttat
     1201 ggcagtgatg cgcgaaaaaa gaaaagcttt aaataaacaa gaacttgacg aagtagcagt
     1261 tggcactacc acaatggatg ctaaaaaacc ggaaatgaaa gatgatgctg aagacatggc
     1321 tttatacacc gacatggtgg aaaaccttgt gcaaactgaa tataacataa aactaaaata
     1381 caacatacct gccgatggca aagaacacat ggtagttata caaaacacag aagtgaaggc
     1441 cgattataat ttctctgcag tacctaaact tgatgccaat gcatatttac aagcacgcat
     1501 aaccgactgg agcgatttga atttattgcc cggcatatcg cgtatatttt tcgacaattc
     1561 ttttgtaggc caatcggtaa ttgatcctta catcacaaac gacaccatgt tgctaagttt
     1621 gggtaaagac aaatcaataa taatgcaacg caaaaaattg aaagataaaa caagacaaaa
     1681 gtttttgggc gaagacaaaa caatttcgta ctcgttcgaa ataagtttaa aaaatacaaa
     1741 atcgttgccg gtaaatatta ctgtcgaaga tcagattcca atttccaata caaaagaaat
     1801 agaagtgaaa ttgatagctg gcaacaaagc taactacaat gtagaaaccg gaaaacttga
     1861 atgggattta caattgaagc caggcgaatc gaaaaaatta ttggttacct acgaagtaaa
     1921 attacccaag aataaaacgg tggcggggat gtagatcggg tcggagttgg ggaagttttg
     1981 aaagttgaga aggttgggaa agttgagaag gtttaaaagt tcaatggttc aaaagttgtt
     2041 tcatgcattc tgattttatt cggaattcac tcatcctaca cttgacaaaa agcatttgta
     2101 tttctacgtg ttctcttttc ctccgcgcga aatgtatttg cattttattc ggaattcatt
     2161 cgtgcattcc ccgacactgc ggtcggggca agcgtggcaa aaagtatttg tatttctccg
     2221 tgttctcttt tcctccgtgc gaaatgtatt cctttaaaga aaattaaaat ctaattgcat
     2281 atttatttga tgacagaaaa agatttcttt tgtgatgcaa ataaaatttg gctatgcaga
     2341 aattcgaaac taccatatta aaatttggaa gcatgggtga aaaaaccgga tggacgtata
     2401 tcatcattcc ggccgatgta gcgcaacagg taaaacccgg caacaaagtt tcgtttaaaa
     2461 taaaaggtaa actcgacaat tataagttca gtgggctaag tgtattgcca atgggtaaag
     2521 gtgattttat tttagcgttg aatgccgaaa taagaaaagc aattggtaag cgccacggtg
     2581 ctatgttgat ggtacaatta gaagaagata aaagtccgtt cataatgaat gcagatttta
     2641 tggaatgctt aaacgatgca cctgatgctg ttgaattttt cgaaacttta agtggttcgc
     2701 atcagcgtta tttttcgaaa tgggttgaca gtgcaaaaac aatagaaaca aaaaccaaac
     2761 gaattgcact cgcaataaat gcgctgtcaa aaaaacttgg ttatcccgaa atgttgcgat
     2821 cacaaaagca aaacaaattg tgattatgct ttttcttctc cagtgattgt gtcttcttct
     2881 ctcgttattg cgagttcttc tccattcatt gagcgttctt ctcccgtcat tgcgagcgaa
     2941 gcgaagcaat ctctgctgcg caaaattcca ttgatgctga gattgcttca gtcgtacctc
     3001 cttcgcaatg acggaaggaa agagtattca ttcgtgcatt cgtggctaaa agtattggca
     3061 taaaaaaacc tgcaactttc attgcaggtt ctcttttcta tttctgattc aaaattattt
     3121 tttctccaaa tcgggttcga acaattcgtc tttactcaca acttttattt tatcgctttc
     3181 attaatcaat ttcgaaatgg taaggtatgg tccggtaata attttttcgc ctccctttaa
     3241 tccggttttt atttctataa aattattgtc ctgtataccg gtagatacca atatgcgttt
     3301 ggcttttcca tcttcaatta caaatacaca ttcttttata ttggcttcat catctttcga
     3361 aggcgcctta tcatcctttt tttcttcctc tttattttca ccacctatgg ctatttttat
     3421 ttttttggca tcagcctttt tacttgtatc acgtgtagtt acacattgta ttggcacagc
     3481 cacaacattt gaagcctcct tggtacgtat ttctaccgat gccgacatgc cagggcgaaa
     3541 aggactttgg tttgcattcg attcattcat gaggtcttta tacgagtcgc gaagtatccg
     3601 cactttcact gtatagtttg ttacctgatc tgtgctttgt tgcatcaacg atgtttttgc
     3661 agcatttgca atttcagtaa caataccttt aaattttctg tcatcataag catcaatttc
     3721 tatatcggca gtatcggaca tgcttacacg tataatatcg gtttcactca catcaactat
     3781 tacttccatt tcgttgagat tcgcaaggcg cataatttcg gtaccatcca tcatatttgt
     3841 accaacaaca cgttcccctt tctctttgct aagcgatgaa attgttccat cgacaggcgc
     3901 atataaaatt gttttgttga gattttcctg ggcttgcttt agcgatgcat ttgcaccttg
     3961 tatgccaaac tccgatgctt ttatattttg caaagcagca tccacttcgg cttttgcaat
     4021 gtcgtatgca gatttagcat tttcaaaatc ttgttgcgaa attactttat catcatacaa
     4081 ttgttttgca cgtttaaatg caaagtcggc tttttcggat tgcgattttg cctgctccaa
     4141 tcgtgatttg cttcctgcca atccactctt acttgtattt accgatgctt ttactctatc
     4201 caaatcggca aattaaatat cgggacgaat ttttactagc aagtcacctt tctttaccac
     4261 ttgcccttct tttacaaaga ggtcaactat ttcgccactt acatcgctag ttatttttaa
     4321 ttctgcttcg ggttgtactt tgccgcttgc tgttactatc tctacaatat tatggcgcga
     4381 aaccaattcc atgcttactt tcttatcgcc actttcgcca atgaggccat tcttttttgc
     4441 aacaacaagc gctatcaaaa gtataacagc tataaggcct atgataaacc atttattacg
     4501 taacatattt ttttgtggtt tgagttttat taaagattta caaagttagt ttttggccgt
     4561 tgtaaaattc caatactttg attcgaaaaa tcaaatcgaa ttttgcctgc aacaaatcgg
     4621 actgtgcacg ggtagcatta tttttggctg tgagatattc gagcgatgtg cccataccgg
     4681 cattaaattt cttttcggaa tagcccgatg ataaaagtgc agcgctgtat gctttttgtg
     4741 ctgcggcata tttcaattgt gccgactttg catcgaggtg tgcttgttgt atggttttaa
     4801 aaatttgctg tttcgtattt tgcaaatcga cattggtacg ggccatattt atttttgcac
     4861 gactgatatt gctgtgtgta ctccatgcat tgaatattgg aatttgtaaa ctaagtccaa
     4921 tgcctttgtt caaattttcg tttacttgtg tgttgaatgg agtttcttca aatacggttt
     4981 taaaacttgg agacaaaaca gtatcgaatg cagatgtaat ttgcccattg ggcgcaaagc
     5041 ctgtaaattg aggtgttccg gccaatcgct ttgcaccact ggaataaccc gaacttacac
     5101 taccaaacgc agtgaggcgt ggtgagtaac ctccgcgtgc aattgacaat gccgtaccgg
     5161 cactttggga gcggatttct gcagctttca cttcgggcat ggcccttaat gcattttcat
     5221 atatttgata agctgtaaat cctactatgc tttggtcggc aagttcaaga ttagggtctt
     5281 ctatatcgaa attttccgca ctgtcgagat tcattatttg caccaatgta agtagcgatt
     5341 gctttacaga gtttgaagca tttatttgtt gcaattcttc tgatgcaacc tgcgcttcca
     5401 aatccatcaa actgccttcg gccaacactc ctgccagcac ttgttttttt attacatcgc
     5461 gttgtttttc tatcgactca atgcgcaatt tagcggcttt caattgttct ttactaaaca
     5521 atacctgtaa gtatgacgat gccacactca acgaaatatc gtttatcact ttttctaaat
     5581 cgtgcttgcc tgcctgataa tcgagcttgc tttgctttac tgtattcatt agttgaaacc
     5641 catcgaacaa taccacattc gcactcagtg aaagctgtgt attagaaatt tgctgattgg
     5701 taaattgata cgtgtatggg tcaacagagc ggccaaaatt tatattctga cttgcactcc
     5761 catttaccga tggcagcaat gctgctttat tttgaatgag cgtaacatgt gtgccttgcc
     5821 ctgcaagtgc agattgctgc accattaaat tattattcat agcatattca acgcattctt
     5881 ttagtgacca acgttgttgt gcctccatat tgaaatgaag tagaaggcat agtgcgaaaa
     5941 cagaaaatgt tctaagcata gtggttttat ttcgaggtaa aagtatatat tgccctttaa
     6001 tatttttact ttataaaaag ttaattttaa gacacttata aattgatatt gttacaaaaa
     6061 agtagttatt attgtgtatg agtaaatgga tagaaacatt agcggctaaa tggaagcttg
     6121 acccgggcga aaagagtaac attgaaacct acgaaagcaa aaaacttttt tccatttatt
     6181 atgagttaca aacgctcata acggttgggg ttttattact tgctacttcg gcagggattt
     6241 ttttatataa aaacatcgac actataggtc attccatttt ggtattgttg actggcattg
     6301 ctgctgtatc gtgtttcgca ttttgcatat tcaaaaaaga aaaacaagca aacattactt
     6361 attacgataa tgttctttac ctcggctgca tgttgcttgt aagttgcgtt ggttactggc
     6421 aatatcagta tggtattttt ggcaaacatc atgcgtttgc cattttagtt ccggcaataa
     6481 taatcacagc atttgcgtat tattttaacg accgggccat tctaagtttg gccattacac
     6541 tttgggcagg ttatttagga tttacaattt cgataccggc aattttgttt gggcaaaatt
     6601 tcagatcgct tcaactcatc tttacagcaa ttatatttgc atgtgtgctt atggcattgg
     6661 catatgcgca ttaccattac aaaaaaaatc ttccttttgg ttttactatt gcaagctttg
     6721 ctttccatat tatgtgtctg gcattgcttg caggtatttt taatcagcct tatagttgga
     6781 tttgggcagc aaccctcgtt gttatttgtt ttgctgctgt gctattttcg ttggtcgagc
     6841 aatcatatgc aacttttgtt tcggcagtgt tgtatacgta cattgctgtt tcttatttgt
     6901 gtatcaattt tttgatgcat catcacattc tcgagtccac ttatttttta ttgatatatt
     6961 ttattgcaac ttccattatg ctgataaaat atattcgtgt gggaaaacaa aaattattta
     7021 aagcctgatg atttataata tagaaaaact gcgcaacaaa ttattgttac ccattgctac
     7081 agaatggatg gcagaaaatg taatggataa aaagaatatt cctttactaa cacaagtgca
     7141 aaaagatgca tacaaaccat cgcaaatagc atttcaggtt gggttatttt tgctcacttt
     7201 tattttggct ttttgcagtg ccggcctgat tgctatgttt tttggattta caaatagcag
     7261 catggctttc ggaatgatat gctgtttttt ttcaatactc agttttgttg cggcctatta
     7321 ttttatcaat acaaaattgc attttaaaac cggggtcgat acagccctgt actattttag
     7381 cattattagc ttgatgcttg gcataggttt aatgctcgat aatttgtgga acaataccag
     7441 caccctcgtt gctttttgta tgctgtgcag tgggttgctt ttgctttacg catttctgtt
     7501 tttcgatttt gtgctggcca tgattgccat tatggtattt gcaacgggat atgcaaatgc
     7561 cttttacgaa acaacagcaa ttataaaact ttgtgccagt ttactttttc catttgtgtg
     7621 ttttgctttt tataaaacca tactctattt cgaaaacaaa atggatgtgg tataccaaaa
     7681 tatttttcct tattgtaaat tcatgctggc agtagttgct tacattgcta tcaaccggtc
     7741 gtttgtaaac gaagtttttt ttgagtatac tttaaaatac ctcaataaaa atgtgtttgc
     7801 tgcattttct attttctctg ttgcatggag ttggctgctt ccgttagttt atatttttta
     7861 cggcttatac aaaaacgaac gttcgtttct tattgcaggc attacatgca tggcattgtt
     7921 catttgttcg attccgtatt ttcatccttc agctattccc gaagtaatga tgctgatagg
     7981 aagtgtatca tgttttattt ttgttggcct gtcaaattat tttttcaaaa acaatgcatg
     8041 gaaaataaca ctacaaaaat cgaaatacga tagtgcaatc ttatcgaaag aaatgcaagg
     8101 cattatcatg tcgcaagcca tagcaaccaa aactatttcg caagacacaa cagaatttgg
     8161 tggtggcaag tttggtggcg ggggtggtag nnnnnnnnnn nnnnnnnnnn nnnttttagt
     8221 attgggtatt gagaatggta gtagtgagta ttgagatttt agtattgagt attgggaatg
     8281 gtagtattgg gtagtgagat aggcagtaat gagaagtgag atggttagta gtgattagtg
     8341 agattgatgg tagtgagcag tgagatcgtt ggtgttgaga taataggttt taattctttt
     8401 taagcagata atttctgtga ggcttctatc ctttgccata aattaatttg cttcatattc
     8461 aagtccgggg aatttttctt tgaatttatt aataaacttg tctatattat ttatacccca
     8521 atttcttgca acaaaataac tttcattatt gtacacaatc tcagattctt tggtcgtttt
     8581 atatttctta tattttatat catactccga taattcttct tgctttttca gcaattgaaa
     8641 actacagctt ttatcttgac gcaggtattc tatattttgg tcgttaataa gattatactt
     8701 ttcaaaaagc tttaatgttt gaagtccaat ttctgctttt ataatattcg atgtaaaaaa
     8761 ttgctcctta tattttatat taaatgtgct gcggtctttg tccgaagata agttatcgag
     8821 tgcttctgta atgctatctc tagtgtcttt ttcttgttca gggtccgaac ttattgcata
     8881 caaagatgct aaaatcaact ttttgttttt ttcccaaaag ctagaaagta gctttctttc
     8941 ttcgttacct aatgccataa ttaattcttg tttgtgcccg tctgctgctt cttcaattga
     9001 aggctgactt agcgatttta gatattctgt taaaatattt tgtgttcgag ctgagatatt
     9061 ttgattcaat gcaggctcta ttaaatagtc tactaaggac tgataattaa tttgaataaa
     9121 ttctttgcaa ttgctttcgg gttctgttaa ttcaatgagt tcaagtgttg aaattggtgt
     9181 taaataaata taaaatatta tattatcttc ggtttctaat ggattaaagt gatggtaata
     9241 gtttgtagtt tggtcgttat tttctttgct tccaacttta ttttcaataa caaattttat
     9301 gtttcttttt tggtccgaaa ttgaaagagc caactcaata taaatatcca gtcttccaac
     9361 atttttaatt gcatattcgg ggtgaactga aatcctttct atggaataat tttcagttat
     9421 taaaaaattg aaaagagcat tatgtttgtt ttttaatttg tcgccactga atgtagttat
     9481 aatgtctaca aatttcctga ttgcaaattc tcctaaatta tgactttcta aattattgaa
     9541 taaccaagca aggaaaccac tatggctgat ttcacgtctg ctaaccccta aaatttcgga
     9601 aaatgatttt gaataataaa agctttctaa cttttgaaaa tcagagtcgt ttttaaattt
     9661 tattatttca tccctaattt ggtctaaggt ataaatattt tctctcattt tttctttcac
     9721 ttaacaagtc caacaatgaa tcatctcaat gcaaaaggtc tgattaccaa aaatctcaat
     9781 actgaatacc atcagaccca ttactatcaa tccaaatact caataccaaa agtctctata
     9841 cccaatacta ttaatcccaa tactaaaaat ctcaatacca aaaaaacact acttccccgc
     9901 caaggcgttc tttaattttg taattacgtt tacttctaca gcatctattc ctttcatatc
     9961 ggcaaggtag caacttatcc agtcgccaat gtgcacaagg taaagggtgc gtgccagttt
    10021 tgattcacct ttcgaccata cttcaataat tgttgatgtt acattcgaag ctacttcttt
    10081 ggtgtagtca agtcgtgttt gggtgcgtat atagtcatcg ttgttgcgca taaaaattac
    10141 ggcaaggttt tcattttttt caacccaccc taccaattca ttgtgattca tttcgggcaa
    10201 tgcatggtgc cagcaaagca ttttggagtt ctcgtttatc tgttgacgga agcgcacaca
    10261 tactccttcg taaccggcat cgctatatag aacagcaatt ttgcccatta ccttttttgc
    10321 tacttccatt gcttcggctt gagttgcttc ttcattggcc tggagcattt ttatcgcatc
    10381 gtttatttcg tctaaaaact tatcgctgca caatcctttt ttattcagca caaaaaataa
    10441 ttgcgtgaat gaataaccaa acattgcgcg gggtggcatt cctccgggaa taataatatt
    10501 ttcgataccg ttggttcgtg ccaggtcaat tatttttcct ccggaagcca cacaaactat
    10561 ttgcgcacct ttttttaatg ccacttcaaa agcctgcaca gtttcttcgg tattgcccga
    10621 gtaagaacaa actataacaa gtgtatgttg attgataaat gctggcggaa aataatcttt
    10681 acatactaca accggcactg caagttcctg gtctaaacat tgcgcggcta tggtgccacc
    10741 tatgcccgac ccaccgagac ctgtaataag cacattgcga atttcgattt ccggttttgc
    10801 aatcgcagca ttttgcccta tttcgattgc ggcaccaagg tgggccgaaa aatcggcaac
    10861 taaatttttc ataacttaat tattagaatt attccggtgc gaaagtaaca atgcctttga
    10921 ttgtaaaaaa tgattgttgt tttaaattgt cattatgcac aaaaacagaa gttatagaaa
    10981 acaaaaaagc gtcctgtgtt tttaatacag aacgctcgat tatgttttgt gcaaatattt
    11041 atgcctctat ggtttcttct tcggctataa gctcgtaaaa ctcattgtaa gcttttatat
    11101 aattttcttc gggatgcttt aagcatggct tgcccgattt tttaatatgt gcagcatact
    11161 cttttggcat atcttctccc atttgaataa atgcgtccga tttcgacatg gcattaagca
    11221 caatgtttga ataggtaggg tctttcaatt ctttcacatc cgaagctgca ataccttcca
    11281 tcatcaattt tttcgaatac tcaggattaa aggtatcctc aaacgaatca ttataaagag
    11341 aataaaccac tttggcattt ttaaaatgtg ggtcttcttt ataacttgtc ttaatgaata
    11401 atggaatcaa actcgtcatc cagccgtggc aatgaataac atcgggctgc caaccaagct
    11461 tctttacagt ttctaatact ccgcggcaat aaaaaatggt acggtcgtca ttgtctttaa
    11521 agtgtttgcc tttggcatcg gtcacagttg ctttgcgttg aaagtattct tcgttatcaa
    11581 taaaataaac ctgcatgcgt gccgattgaa tacttgctac cttaataatg agtggatggt
    11641 cggtatcgtc aataatgagg ttcattcctg agaggcgtat tacttcgtgt aattggttgc
    11701 gtctttcgtt gatggttccg tacttgggca taaacaccct aatttctttt cctgattcct
    11761 gagttccctg gggtaagtag cgacaaattt tcgataattc tgtttcgtct aggtaagggg
    11821 aaatttcttg tgtcacaaag agcactttcg tttttttcat catatctttc tattttatct
    11881 taattgaggc gcaaaagtaa taataaatta atcgttaaac aacaaagaca acgagtaata
    11941 aacaagtatg gtaattaagc atatgggtta atggcttaaa taaagtggcg aatagctaca
    12001 aatagccaac taatggtttt aatgtgtata aaaatgcctg ttaaatattg cttagcccgt
    12061 tcatttagtc gatggt
//
LOCUS       DAORQA010000037        12071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1427_length_12071_cov_9.320489, whole genome shotgun sequence.
ACCESSION   DAORQA010000037 DAORQA010000000
VERSION     DAORQA010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12071
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1427_length_12071_cov_9.320489"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2343
                     /locus_tag="PK736_04135"
     CDS             <1..2343
                     /locus_tag="PK736_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32612.1"
                     /translation="TLDYQKAHPHFASKTNQFGGNWTSKGPNAAAGGYSGLGRITCIA
                     FHPTNNSTYWVGTPAGGLWKTTNNGQTWTTNTDNLPVLGITSIAINPTNPNTMYIATG
                     DGDLGNLSGVQNGSYFYGTGDTKSLGVFKSTDGGATWNTTGLSWNTSQIKQTHKLLIN
                     PNNPAILLCASSDGIFRTTNSGTSWTNVASGNFNDIEFKPGNPSVVHAATFDIFGANA
                     QIFLSTNGGVSFTQQTAFTAVTRLDIAVSAALPNLVDVVGIDQQGGLAGIVYSDNSGL
                     TYQQYIWGDCQYNLIASDPGAQGCGGQGMYDLYYVINPQDEYEIWLGGVNTWATPDGG
                     TNYYLQTFWNGSNQNPGVPEVHADQHFFAFHPNANGTQFVTNDGGIYKSTNGGGSWID
                     ITNGMQISEMYRIAASQSTSNKVLCGLQDNGSKYLAGTNWSDVTGGDGTDCAIDYTND
                     NIQYGGYVYGQLFRTTDAWQTQTTISNNIPGTPQGAWITPFEIDPVTPATIYAGYDKV
                     YKSTNKGNSWTAISPTLTTRKLNTLQVAPSNTQHIYAANWDTLFSSTNGGGNWYADPV
                     ALSNAFITGIAINPNNDNNIWISLSGYASGQKVIVSYDGGLSFSNYSGSLPNLPINCI
                     IYENNTANGLYVGTDVGIFYRDDNTGDWIPFSNQLPNVPVADLDISYNDGKLWAGTFG
                     RGLWKSDLFSASVNIAENDVFENVSVYPNPAKDLLTIKSIKEIEQIKLMNGIGQILFD
                     EKYENNTAMVNIDLKNYPPSVYTLVAIIDGKIKSYRVIKN"
     gene            2579..5032
                     /locus_tag="PK736_04140"
     CDS             2579..5032
                     /locus_tag="PK736_04140"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HRC32613.1"
                     /translation="MFVQSNIFTLNNSVDFTNFAEEAIAISVDKQNLYDLSKENNDAL
                     RMLIPMANNQSIELELIKSNIEAEDFQVGTKGIDSRENILYKGGLHYRGIIAGNNQSI
                     AAVSIFENEIFILFADDAGNYNLGKMRDGSENYMLYMDNKLKVKPEFECGTNDYAEPV
                     DDNNVQSNAKVLNNCKIVKCYYECDFAMYTAFNSNTTSTVNYLTSLFNQKATMYANEN
                     INIGISQIYVWTSTDPYVTKTSTSTLNAEFSKLTGNTFNGDIANLITTRSIGGGIAQG
                     FSATCNKAAAHCTSFIYTTFAVYPTYSWSVMVLTHEMGHLMGSRHTHACVWNGNNTSI
                     DGCAGFVEGSCSLLGYPSGGGTIMSYCHTQSVGINFTKGFGPLPSALIYNKYNTATCY
                     EGSATLTPKNLTTTNITATGAKLNWTAVNGATTYTIEYKIGSSQKWKVFGTATTNTKT
                     LTGLTPGVSYNWRVKSGCSPFSASKTFLTLTKAGCAIATNLASINITSTTAQLYWSKY
                     ATATKYRLSYRLTGTTAWTTKVVTPSNYLLTGLTGFKTYQWKIDTYCGAAYLASSAID
                     SFKTQTTNAPTTYCTPSTSGTQYEYMNSVVFSNVSKVSGNDNGYGNYTTLVANAVVGT
                     TYPISVNIWKSSLNSLYSVRAWIDYNRDGDFDDANEVVVTVVNSALSTFVANVVIPTG
                     VNYGKTRLRIAFSSGTLIPTPCNYGAGGEYEDYTVLLKAAPSPKISFDNSDTAADSFI
                     SLSPNPASNEININWENNDETYENATVINSLAEIVLAQRIENGLNEIRLDIRSLTSGI
                     YFIKLSGVNNSTIHKFVKQ"
     gene            5449..8520
                     /locus_tag="PK736_04145"
     CDS             5449..8520
                     /locus_tag="PK736_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32614.1"
                     /translation="MKKINLYLSMMLLALCGFATNAYAVAESEPNDVWNQADVITLGA
                     TGTGTAGLSQNQDWWRVTSAVDGKLTVNWTATNSLNIYCQIYDTLGVVQYASAYTSGS
                     SLIEKDGLAAGTYYIKMIAFYSTEAPTYSFVPTWTTPAQLIDLEPNNTRAQAKTLPLN
                     GSKTGHIGYVFNQVDDLEDWWKVTTNLDGKLEWTITVNNSGNNVYAQLYDNNGTTLFG
                     GGYTSGTATYSIDGLSAGTYYIKIRNFYTTEYAPYTISNNLVLPPVINDIEPNGLYTQ
                     ALTIAINDSAVGHIGYYYNNLDDTLDWYKTTTTLEGRIDIRMRVLNGGNNVYMELFEG
                     NGTTFLTGGYTSSDGTWSKDGLAAGTYYIRVRTFYNSEFAPYKMNVNLIPAIVTIDTE
                     PNGTRALAKTINVNDSITGHIGYSNYLLKDTFDYRKITLTQYGTLAWKLNVLNGQNVY
                     AELFDNNGTTKLEGSYTSSTATYSRPDLAPGTYYLRIRNFYNYEFSPYALKTSFVAVG
                     TDAEPNGTQATASTLPLNATSNYCVGYLYNGVRDTNDWHKVILPHDGKLSITLTGNIY
                     NTYLEVYAENGTTNLYSQYTTAGTLNWTKDNAAGTYYVKVKQFYTQYYSTYQLTAAHT
                     TNNNNDGEPNKYAKQGALICGYATKAGNIGYTSGTGTVDNTDWFKLGYYTGAPLVINI
                     AKVARTWDGNGNADVKYYLYKDTTAAPVDTAVMNGATGYTKSYAALTNGAYYIKMLRT
                     AGYGGYTINAQYKDTCSHTVALVKSAGGSLCSKGSLTYYITRGRAPYSVQLYKDGAVY
                     GAAVSTSDTARYTLLPPGNYYCRVKGNGAITYYVQSSSKAISPKPQATNATGITKNQA
                     TITWTSLACVDGYIVSYKKSTDVTFKNDTLPAGSIADTLKSLAASTIYNYKVQSFHKV
                     GNSYYKGSFSTLKSFTTLAAREGEYTAMVINDNRLNIYPNPATSVLNLDINSDEVLTA
                     TITNSLGQTIKAIQIYANGTLSIDIADLKSGIYSLQLSNGNQLQSATFIKE"
     gene            8703..>9533
                     /locus_tag="PK736_04150"
     CDS             8703..>9533
                     /locus_tag="PK736_04150"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC32615.1"
                     /translation="MNDKAEQLYEQALHLIDFNRFDKAAQLLAQALAINPNYHEAFGQ
                     LAYIELQNKNLNKALEHALSCLACNAGYAYGHYIHAIILYKQGAYNKSYIAIVSALEL
                     SPNFAAAHDLLADIYLKKGKIEEAINSINEAIKLDPENSSYHASKSQLMKLVGDKTGA
                     ANSINTGLQFDIEDVETWYQKGKMELEGGFINEALECFQNALRLNPQNEVLKDHMLRS
                     IKMQNSFLFSVEKYTEIKFTVSGKSAWLMLIPQFWGGVFFRAFWAQICNYISSFADLF
                     Y"
     assembly_gap    9534..9549
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9781..9790
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10037..11095)
                     /gene="serC"
                     /locus_tag="PK736_04155"
     CDS             complement(10037..11095)
                     /gene="serC"
                     /locus_tag="PK736_04155"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010134146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRC32616.1"
                     /translation="MQVHNFGAGPSILPREVIEQSAAGVLNFNDSGMSILEISHRSEL
                     FEKVLNEAISLVRELLNVPENYHVLFLSGGASFHFTMVPQNLMKTKAAYVESGVWAKK
                     AIKESQLFGDTKVIASSADKNFTYLPAIENIPADADYLHCTSNNTIYGSQMKSFPVTT
                     IPLVCDMSSDFLSRPVDVSQFGMIYAGAQKNVGPSGTSIVIIRNDLLDGYKTNTPIIL
                     RYKTHVDANSLYNTPPVFAIYTCMLTLRWLKKLGGLKAMEAINNKKAELLYNALESNS
                     LFNLPVAKADRSYMNVVFTIDDKEKEKAFLDFCKSKNIVGIKGHKSVGGFRASIYNAL
                     PIDSVQFLVDVIAEFTAQ"
     gene            complement(11140..>12071)
                     /locus_tag="PK736_04160"
     CDS             complement(11140..>12071)
                     /locus_tag="PK736_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32617.1"
                     /translation="LSLAVIIGIYFSFNLNAQSTSFKYYTLSNAFKNSLFYIKCLSDS
                     VARSTDDSTKLFANAKLRDTLPGLLCVETTFEDPFDSIKSISIIKSNDEHVRIYTWFV
                     ATENATKYHYEGVIQFRDKGDKLFKVISLRDSTYEDDLVIKKLLKKGNWYGAMYYNIV
                     EVRKNGNTLYTLIGWKGYDKNVTKKVIDIVKFTRGEPTFGAAVLQNKKKIQSRLIFSY
                     SSAVSMKLNYDSKKKMIVYEHLSAPDGTENAAPEVMGPDMTYDGLKWRDEKWMYFKDL
                     EMKNNSTESDKKDKPDKKEKKLYTPASEEGEKK"
BASE COUNT         3915 a         2245 c         2272 g         3613 t
ORIGIN      
        1 accctggatt atcaaaaggc acatccgcat tttgcaagta aaacaaatca gtttggcggt
       61 aactggacaa gcaaaggacc caatgctgcg gcaggtggtt actcgggctt aggtcgtata
      121 acatgtatag cattccatcc tacaaataat tcaacatatt gggttggtac tcctgcaggt
      181 ggcttatgga aaactaccaa taacggccaa acctggacta ccaataccga taatcttcca
      241 gtgcttggca ttaccagtat tgctattaat ccaactaatc caaacacaat gtacattgca
      301 accggtgacg gtgatttggg taatttatcg ggtgtgcaaa atggctcgta tttttatggt
      361 accggtgata caaaaagttt aggggtattt aaaagtaccg atggtggtgc tacttggaat
      421 acaacaggcc tgtcgtggaa tactagccaa ataaaacaaa cgcataaatt attaattaat
      481 ccaaacaatc cggcaatact tttatgtgct tcgagcgatg gaatttttcg cactacaaat
      541 agtggtacat cgtggacaaa cgtagcatca ggaaatttta atgatataga gtttaagccc
      601 ggcaacccga gtgtagtgca tgcagcaacc ttcgatattt ttggtgccaa tgcacaaatt
      661 tttctttcta caaatggtgg tgtttcattt acgcaacaaa cggcattcac cgcagttacc
      721 cgccttgata ttgcagtaag cgcggcattg ccaaatttgg tagatgtagt tggcatagat
      781 caacaaggtg gcctggccgg aatagtatac tctgataata gtggcttgac ttaccagcaa
      841 tacatatggg gcgattgtca atataattta attgccagcg atccgggtgc gcaaggctgt
      901 ggtggacaag gcatgtacga tttatattat gttataaacc cacaagatga atatgaaatt
      961 tggttgggtg gggtaaacac ttgggctact cctgacggtg gtaccaatta ttatttgcaa
     1021 actttttgga atggctcaaa tcaaaatccc ggtgtacccg aagtgcatgc cgatcaacat
     1081 ttttttgcct ttcatcccaa tgcaaatgga acgcaattcg taaccaatga tggtggcatt
     1141 tataaatcga caaatggcgg tggtagttgg atcgatatta ccaatggtat gcagataagt
     1201 gaaatgtatc gcattgcagc atcgcaaagc acatcgaaca aagtactgtg tggcttgcaa
     1261 gataacggca gtaaatattt agccggcacc aattggagcg atgtaactgg tggcgatggt
     1321 accgattgcg cgatagatta taccaacgat aatattcagt atggtgggta tgtgtacggg
     1381 caactattta gaaccacaga tgcatggcaa acacaaacaa ccatatcaaa caatattccc
     1441 ggcacaccac aaggtgcatg gattacccct ttcgaaattg accctgttac tccggccaca
     1501 atttatgccg ggtacgacaa ggtgtataaa agcaccaata aaggaaattc gtggacagca
     1561 atttcgccaa cgcttaccac acgcaaatta aatactttgc aagtagcgcc ttccaataca
     1621 caacatattt atgctgctaa ttgggatact ttatttagtt cgacaaatgg tggcggcaat
     1681 tggtatgccg accctgttgc ccttagcaat gctttcatta caggtattgc catcaatcca
     1741 aacaacgata ataatatttg gatttcatta tcgggttatg caagtggaca aaaagtaatt
     1801 gtaagttatg acggaggatt aagtttttcg aactacagtg gatcgttacc taacttgcct
     1861 attaattgta tcatctatga aaacaatacg gcaaatggct tgtatgtggg taccgatgtg
     1921 ggtatctttt atcgcgatga taatactggc gattggatac cattcagcaa tcagttgcca
     1981 aatgttcctg ttgccgattt agacatttca tataatgacg gtaaactatg ggccggcact
     2041 tttggccgtg gcttatggaa gagtgatttg ttttcggcaa gtgtaaatat tgctgaaaat
     2101 gacgtgttcg aaaatgtaag cgtatatcca aaccctgcca aagatttatt aactattaaa
     2161 tcgataaagg aaattgagca aataaaatta atgaatggaa tcggtcaaat actatttgac
     2221 gaaaagtatg agaataacac tgcaatggta aacatcgatt taaaaaatta tcctccatca
     2281 gtatatacgc ttgtggctat tatcgatgga aaaataaaat catatagagt aattaaaaat
     2341 taaatcaaaa gcatttaaat tcttagtata aaaaataatg cattcataaa taataaaata
     2401 tttaaaaaca gtaatcaaaa ataaatgaaa aaaatcaaat tgtcctttat cgcaatagct
     2461 tgcctattgt ttgtgcaaaa tagctatgcg caaaatgcaa attcaaatat cgaagacgag
     2521 gtttcggatg cgccaaatat gttatggcaa atggtagaac aagcaaaaaa acgatgtcgt
     2581 gtttgtacag tcaaatattt ttacgctaaa taatagcgtt gattttacaa attttgccga
     2641 agaagccata gcaatatcgg ttgataaaca gaacctgtac gatttatcaa aagaaaataa
     2701 tgatgccttg cgtatgttga ttcccatggc taataatcaa agtattgagc tggagttaat
     2761 aaaatcgaac attgaagccg aagattttca agtaggtaca aaaggaatcg atagtcgtga
     2821 aaatattttg tacaaaggtg gactacacta tcgtggaatt attgccggca ataatcaatc
     2881 aattgctgct gtaagtattt tcgagaacga aatatttatt ctttttgctg atgatgccgg
     2941 caattacaac ttaggtaaaa tgcgcgatgg gtcggaaaac tatatgttat atatggataa
     3001 taaactaaaa gtaaagcctg aatttgaatg cggcacaaac gactatgccg agccggtgga
     3061 tgataataat gtgcaatcaa atgccaaagt acttaataat tgcaagatag ttaaatgcta
     3121 ttatgagtgt gactttgcaa tgtatacagc ttttaatagt aacactactt caacagtaaa
     3181 ttatttgact tcgcttttca accaaaaagc aacaatgtat gcaaacgaaa atatcaatat
     3241 tggaatatcg caaatttatg tttggacaag cactgacccc tacgttacaa aaacttccac
     3301 cagtaccctc aatgccgagt tttcgaaact tacaggtaat actttcaatg gtgatatagc
     3361 aaatttaata acaacaagaa gcattggtgg cggcatagca caaggattta gcgccacttg
     3421 caataaagcg gctgcacatt gcacatcatt tatttatacc acgtttgctg tatacccaac
     3481 ctactcgtgg agtgtaatgg tgttaacaca cgaaatggga cacttaatgg gatcgcgcca
     3541 cactcatgct tgtgtgtgga atggtaacaa tacttcaatt gatggttgtg ccggatttgt
     3601 agaaggttct tgttccttgt tgggatatcc ttcgggtggc ggcactatca tgagctattg
     3661 ccacacacaa agtgtgggca ttaatttcac aaaaggtttt ggccctttgc catcggcatt
     3721 gatatataat aaatacaaca ctgcaacttg ttatgaaggc agtgccacac ttacaccaaa
     3781 aaatttgact acaaccaaca ttacagccac aggagcaaaa ttaaattgga ccgcagttaa
     3841 tggagcaaca acatatacta tcgaatacaa aattggatct tcacaaaaat ggaaagtttt
     3901 tggaactgca actaccaaca caaaaacact tacaggttta acgcccggtg tatcatacaa
     3961 ttggcgagta aaatccggct gctcaccatt ttcagcttca aaaacattct taacattaac
     4021 aaaagccggt tgtgctattg ccacaaattt ggcttccata aatattacgt caacaaccgc
     4081 acaattgtat tggagtaaat atgcaactgc tacaaagtat cgtttaagtt atcgtttaac
     4141 cggcaccaca gcctggacta ctaaagtggt aaccccttca aattatttgc ttacaggcct
     4201 caccggtttt aaaacgtatc aatggaaaat agatacttac tgtggcgcag cttatttagc
     4261 atcatcggct atcgattcgt ttaaaacaca aaccacaaat gctccaacta catattgcac
     4321 cccatcaaca tcgggcaccc aatatgagta tatgaatagt gtagtattca gtaatgtaag
     4381 caaggtatcg ggcaacgaca atggctatgg aaattatacc acacttgttg ctaatgcagt
     4441 agtaggaaca acatatccaa tatcggtaaa tatttggaag tcgtctttaa actcgctgta
     4501 cagtgtgcgt gcatggatag attacaaccg cgatggcgac tttgacgatg ctaatgaagt
     4561 ggtggtgact gttgtaaatt ctgccctttc tacatttgtt gcaaatgtgg taattcctac
     4621 cggagtaaat tatggcaaaa cgcgtttacg cattgctttc agcagtggca cactaatacc
     4681 tacaccatgc aactatggcg caggcggtga gtatgaagat tatactgtac ttttaaaagc
     4741 tgcaccatca ccaaaaataa gtttcgataa tagcgacacc gcagctgatt cgtttatttc
     4801 tttatcacca aatccggcat cgaacgaaat aaatatcaat tgggaaaaca acgatgaaac
     4861 ttacgaaaat gcaacagtaa ttaattcgct agctgaaata gttttggcac aaagaattga
     4921 aaatggttta aatgaaattc gtttggatat acgttcgtta accagtggca tttattttat
     4981 taaattgtca ggagttaata attccaccat ccataaattt gtgaaacaat aattggattg
     5041 gagttaaaca cacactacta caaattgagt attgattcgg cattcaatat ttagcttctt
     5101 tgtaatatag tttgattcct aaatgagata gttttaaaag ccataattta gaaaacatag
     5161 cggttcgcaa attatattgt ctggcgtgtt tcgcttgcga ccacagagtt ttaagattga
     5221 agtgtgtgta aaacaaagct cagataattt gcaaggtacg tagttggtgg aacatatgct
     5281 cgacaaaaat cacgaatatt cgtgatacca gataaaataa aatgtaatca tttttgctca
     5341 gcgattttag attcccgatt ctcaatgtgc tttcatatta tttctataat ctagtgttaa
     5401 atatcaacat tcataaatta aatcaataac taacaaaaac aaaaaaaaat gaagaagatt
     5461 aatctttact tatcgatgat gttgcttgcc ctatgcgggt ttgcaaccaa tgcttatgca
     5521 gtggcagaaa gcgaaccaaa cgatgtctgg aatcaggccg atgtaattac ccttggtgca
     5581 actggaacgg gtacagctgg tttatcacaa aaccaggatt ggtggcgggt aacctcagcc
     5641 gtagatggaa aacttactgt aaactggaca gcaacaaatt cactaaacat ttattgccag
     5701 atttacgata ctttgggtgt tgttcaatat gcaagtgcct atactagtgg atcctcccta
     5761 atagaaaagg atggtttagc agccggtact tactatatta aaatgattgc tttttattct
     5821 accgaagccc ctacttacag ttttgtgcct acatggacaa cacctgcaca attgatagat
     5881 ttagagccaa acaataccag agcgcaagct aaaacacttc cacttaatgg ttccaaaacc
     5941 ggacatattg gttatgtatt taatcaggta gatgatttag aagactggtg gaaagttact
     6001 acgaatcttg atggaaaact ggaatggacc atcactgtca ataatagtgg gaataatgta
     6061 tatgcacaat tatatgataa caatggaaca acattatttg gtggcggtta tacatctggt
     6121 accgcaacat attctataga tggattatcc gctggtacgt actatattaa aatcagaaat
     6181 ttttatacaa cggaatatgc accctatact atttccaata atttagtatt gccacctgta
     6241 ataaatgata ttgagcctaa tgggttatat actcaggcat taaccatagc tataaacgat
     6301 tcggctgttg ggcatattgg ttattattac aacaaccttg acgatacact cgattggtat
     6361 aaaaccacca ccacattgga aggtagaatt gacattagaa tgcgtgtgct caatggagga
     6421 aataatgtgt atatggagtt attcgaaggc aatggtacta cctttttgac aggaggttat
     6481 acatcctcgg atggcacttg gagcaaagat ggattagctg ccggcactta ttatattcgt
     6541 gttagaactt tctataacag cgagtttgct ccttacaaaa tgaatgtaaa tttaattcct
     6601 gctattgtaa ctatcgatac agaaccaaat gggactcgag ctttggctaa aacaattaat
     6661 gtcaatgatt ctattacagg acatataggg tattcaaatt atttgcttaa agatactttc
     6721 gattacagaa aaattacttt gacacaatat ggcacacttg cttggaaatt aaatgttctc
     6781 aatggacaaa atgtatatgc ggaattattt gataacaatg gaacaacgaa gttggaaggt
     6841 tcgtatacat ctagcacggc aacatattcc cgtcccgatt tagcacccgg cacttattat
     6901 ttacgcatta gaaattttta caattatgaa ttctcacctt atgctttgaa aacttcgttt
     6961 gtggcagttg gtaccgatgc cgaaccaaat ggaacgcagg ccacagcatc tacgctacca
     7021 ttgaatgcaa cttcgaatta ttgtgtagga tatttataca atggagtacg tgatacaaat
     7081 gactggcaca aagttatttt acctcatgat ggtaaactta gcattacact aacaggcaat
     7141 atatacaaca cttacctcga agtatatgcc gaaaacggaa caacaaattt atatagtcag
     7201 tatacaacag cgggcacact caattggaca aaagacaatg ctgccggcac ctattatgta
     7261 aaggttaagc aattttatac tcagtactac tcaacctacc aattaactgc cgcgcatacc
     7321 accaataata acaatgatgg tgagccaaat aaatatgcaa aacaaggggc tttgatttgt
     7381 ggatatgcca ccaaggcggg caacataggt tatacaagtg gaacaggtac agttgacaat
     7441 accgattggt ttaaattagg ttattacacc ggtgcaccac ttgtaattaa tatagcaaaa
     7501 gtagcacgca cgtgggatgg caatggcaat gccgatgtaa aatattattt atataaggac
     7561 acaacagcag cacctgtaga tacggctgtg atgaatggcg caacaggtta cacaaaatca
     7621 tacgctgcac ttacaaatgg tgcctactat ataaaaatgt tacgcacagc cggttacggt
     7681 ggttacacaa ttaatgccca atacaaagat acttgtagcc atacagtagc actggtgaag
     7741 tcggcaggtg gttcgctttg cagcaaagga tcgctcacat attatatcac ccgtggtcgt
     7801 gctccatact cagttcaact ttataaagat ggcgcggtat atggtgcagc agtatctaca
     7861 agtgatacgg caaggtacac gttattgcct ccgggaaact actattgcag agtaaaagga
     7921 aacggggcaa tcacatatta tgtgcaatca tcttctaaag caataagccc caaaccacag
     7981 gctacaaatg caacgggcat tacaaaaaat caggcaacca ttacatggac aagtttggct
     8041 tgtgtagatg gctacattgt aagctacaag aaaagcaccg atgtaacatt taaaaatgat
     8101 acattacctg caggaagcat tgccgatact ttgaaatcac ttgcagccag caccatttac
     8161 aactacaaag tacagtcatt ccataaagtt ggtaactctt attataaagg ttctttctca
     8221 acacttaaat cctttactac acttgcagca cgcgaaggtg agtatacagc aatggttatt
     8281 aatgacaata ggttgaacat atatccaaat ccggcaacca gtgttttaaa tttggatatt
     8341 aattctgatg aggtgcttac cgctaccatt accaattcgt taggccaaac aataaaggcg
     8401 atacaaatat atgccaatgg cacattgagt attgatattg ccgatttgaa atcgggaatc
     8461 tatagccttc aattgagcaa tggcaatcaa ttgcaaagcg ctacattcat aaaggaataa
     8521 ttcgattact tatcttctca ttttgaaaaa gagttgcttc gaaagaggca actctttttt
     8581 tattcttatc agagtctatc ataccattag aaaacaaaaa ataaatgaaa gtctaaatag
     8641 tttgatgtag ccgtcccttt ttaaaaaaaa aatcttcaaa tatttcttta tataattgtg
     8701 ccatgaacga taaggcagaa caattatatg agcaggcatt gcacttaatc gattttaacc
     8761 gtttcgataa ggctgctcaa ttattagcac aggcattggc cataaacccc aattaccatg
     8821 aagcatttgg acaactggct tatattgaat tgcaaaataa aaatctgaac aaagcacttg
     8881 aacatgcttt aagttgtttg gcatgcaatg ccggatatgc ttatggccat tacattcatg
     8941 ctatcatttt gtataaacaa ggtgcatata acaaatcgta tattgccata gttagcgcac
     9001 tcgaattatc gcccaacttt gcagcagctc atgatctgct tgccgatatt tatcttaaaa
     9061 aaggaaaaat tgaagaggcg ataaacagca taaacgaagc cataaaactc gatccggaga
     9121 attcatcgta ccatgcaagc aaatcgcaat tgatgaaatt ggtgggtgat aaaacgggtg
     9181 ctgctaattc aattaatact ggtttgcagt tcgacattga ggatgttgag acatggtatc
     9241 aaaaaggaaa aatggaactt gagggcggtt ttattaatga agcattagag tgttttcaaa
     9301 atgcattgcg attaaatcca caaaacgaag tattaaaaga ccacatgttg cgcagcataa
     9361 aaatgcaaaa tagttttttg ttcagcgtag aaaaatatac cgaaataaaa tttaccgtaa
     9421 gcggtaagtc ggcatggcta atgctgattc cgcaattttg gggtggagtt ttcttccggg
     9481 ctttctgggc gcaaatttgc aattacattt cttcgtttgc cgatttgttt tatnnnnnnn
     9541 nnnnnnnnnc ttcaaattta tttactgcca gccgaaaacg cagaatgata gtttcggcaa
     9601 ctgtaattgc aggatttttt cttgctatta ttatagccat atcattaaaa aactcatccc
     9661 tgcttttctt tagttatttt ttgattgctg ccctgtatcc ggtttatgcc ttgaattcca
     9721 ataaacccga caagtatgat gcaatagtgt ctattttatc attgttggtt tttgttacgg
     9781 nnnnnnnnnn aataaattac agattgtatt tatcaccaac atttgttgca gttcctgtta
     9841 ctattcttgg ccatttattt ttcaatattt tttactttat ccgtttcaaa atatttggtt
     9901 tcaacagaca gtattacgaa ttggaatgag cagtatagtc aataatattt cagtgtgcta
     9961 aagaactatt tgcaaaatag taattgatac tctgtaaaga cttaagctaa taaataaata
    10021 agaaacttgc aaatgcttac tgcgctgtaa actccgcaat aacatcaacc aaaaactgaa
    10081 cactatctat aggcaatgca ttgtaaatgg aagcacggaa accgccaaca cttttatgtc
    10141 cttttattcc aacaatattt tttgatttgc aaaaatcgag aaatgctttt tccttttctt
    10201 tatcgtctat tgtaaacaca acattcatat acgaacgatc ggcttttgca accggtaaat
    10261 tgaacaatga attactttcc aaagcgttat aaagaagttc ggcctttttg ttgttgatgg
    10321 cttccatggc ttttaagcct ccaagttttt tcaaccaacg caatgtgagc atacaagtat
    10381 atattgcaaa aaccggtgga gtattgtaaa gtgagtttgc atcaacatgc gttttgtatc
    10441 gcaatattat aggtgtgttt gttttgtagc catccaacaa atcgttgcga ataattacaa
    10501 tggatgttcc tgatgggcca acattttttt gtgctccggc atatatcatc ccaaattgcg
    10561 aaacatctac agggcgcgat aaaaagtcac tactcatatc acaaaccaaa ggtattgtag
    10621 taacaggaaa acttttcatc tgcgaaccat aaattgtatt gttcgatgtg caatgcagat
    10681 aatcggcatc ggcaggaata ttttcgatag caggtaaata cgtaaagttt ttatcggccg
    10741 atgaagcaat cactttagtg tcgccaaaaa gttgcgattc cttgattgct ttttttgccc
    10801 atactcccga ttctacatag gctgctttgg ttttcatcaa attttgtggc accatagtaa
    10861 agtgaaaact tgccccacca cttaaaaata aaacgtggta attttcgggt acattgagca
    10921 actcacgcac gagtgatatt gcctcgttca ataccttttc gaataattcg ctgcggtgcg
    10981 aaatttctaa aatcgacatt cccgaatcgt taaaattgag cacccctgct gctgattgtt
    11041 ctatcacttc gcgcggtagt atcgatggac cggctccaaa attatgtact tgcatttcct
    11101 gtttcttaaa tatttaaaat taaattttgc gcaaatgtat tatttctttt caccttcttc
    11161 ggaggctggg gtgtatagtt ttttctcctt tttgtcgggc ttatcttttt tatcactctc
    11221 ggtcgaattg tttttcattt cgaggtcttt aaagtacatc catttctcat cgcgccactt
    11281 cagtccatca taagtcatat cggggcccat tacttcgggt gcggcatttt cagtgccatc
    11341 cggtgccgac agatgttcat atactatcat ctttttcttg gaatcgtaat tcaacttcat
    11401 actcactgcc gatgaatacg aaaatattaa acggctttga attttctttt tattttgcaa
    11461 aacagcagct ccaaatgttg gttctcctcg tgtgaatttt acgatgtcta tcactttttt
    11521 agttacattt ttatcgtacc ccttccaccc tattaatgta taaagtgtat ttccgttttt
    11581 acgtacctct acaatattgt agtacatggc accataccaa ttaccttttt ttagtaattt
    11641 ttttatcacc aaatcatctt cataggtgga gtcgcgcaac gatataactt tgaaaagttt
    11701 atcgccctta tcgcgaaatt gaattactcc ctcgtagtga tattttgttg cattttcggt
    11761 agccacaaac catgtatata tgcgtacatg ttcatcgttg cttttgatta tagatatgct
    11821 ttttatcgaa tcaaaaggat cttcaaaagt ggtttctacg cataacaagc cgggcaaggt
    11881 atcgcgcagt tttgcattgg caaataattt tgtgctgtca tcggtactcc gcgcaacact
    11941 atcgctgagg catttgatat aaaacaagct atttttaaat gcattgctta aagtgtaata
    12001 tttaaaactt gttgattgtg catttaaatt aaaactaaaa taaataccga taataactgc
    12061 caatgataat t
//
LOCUS       DAORQA010000038        12034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1429_length_12034_cov_16.653727, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000038 DAORQA010000000
VERSION     DAORQA010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12034
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1429_length_12034_cov_16.653727"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..986
                     /locus_tag="PK736_04165"
     CDS             240..986
                     /locus_tag="PK736_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRC32618.1"
                     /translation="MSHNLVIIPTYNECENAESIIRAVLALATPFHILIVDDNSQDGT
                     ATIVKNLQKEYEGRLHIEERAGKLGLGTAYIHGFKWALQRNYDYIFEMDADFSHKPSD
                     LLKLHKACAEEGADLAIGSRYSNGVTVVNWPIGRVIMSYYASVYVRTILGVPIHDTTA
                     GFICYKKRVLQMINLDKIKFTGYAFQIEMKFTAFKHGFKIVEVPIIFMDRKLGKSKMS
                     SGIFSEAFFGVIELKIKSWFRKYEQARDSF"
     gene            complement(1236..2804)
                     /locus_tag="PK736_04170"
     CDS             complement(1236..2804)
                     /locus_tag="PK736_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32619.1"
                     /translation="MKQIKIITAFAFAISAFQMQAQVTPTWADNVACILYTRCTSCHN
                     PNGAAPFSLMTYTDAFNFAEDIDSSVQAKRMPPWPPNHSYQTYAHERVLTQDEIDIID
                     QWVAAGSPQGNAANAPTPPVYSGISQMPNPDLNTKMPTYQIPPITSDLYRCFVIPNVT
                     SIDKFISGMEVLPGNRNIVHHVLVFQDQGNTVINLDNADTLPGYTSFGGIGSNTAELI
                     GLWAPGAQPYFLPQAMGIKLKANSHIILQIHYPLGTSGQYDSTQVNFKYSTTPFTRQV
                     NIDPILNHDDLNEGFLFIPADSVRTFTCDYTVPIDVTVLSVAPHMHLVGTEISARGVT
                     PLNDTLKLIDIPKWDFHWQGAYDFRQPVKVPSGTTLHSRAFYDNTANNLNNPNTPPKA
                     VYLGENTTNEMMLIYFFYTYYLPGDENIVVDTNMTVQSYNGCNFSLGGTEAWMQQQYS
                     VAPNPATNSITVIPGWQQNYEVRIQDMLGKTVKTISAVSGSANIDINDLAPGMYNAQL
                     FANNKLYNTKFVKQ"
     gene            complement(3127..3666)
                     /locus_tag="PK736_04175"
     CDS             complement(3127..3666)
                     /locus_tag="PK736_04175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRC32620.1"
                     /translation="MRIVIVSGSMRHESVTNRVALCLHNQLTQLKLHSDIIDVREINL
                     PFITDGFANKEAVPDEHKQWANLMFEADAVIMLTPEYNGSYTPSLKNVFDHFPKQSRK
                     AFGIVTASPGGLGGIRAAMQMQQLVPALFGILSPYMLVIPAVDKKFDEQGTLIDPNFQ
                     KSIDLFLREFLWLATQLKK"
     gene            complement(3961..>4472)
                     /locus_tag="PK736_04180"
     CDS             complement(3961..>4472)
                     /locus_tag="PK736_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRC32621.1"
                     /translation="QQVQGSYSKLKSYALKLTQNMEDAEDLTQDTMLKAIANQDKFQD
                     GTNLKGWMYTIMKNVFINNYRRNKKSNITHDDTDNQYYINNTSFTTGNEATSKLALKD
                     INNAIGLLADNLKIPFLMSFTGYKYDEISNYLSLPLGTVKIRIHCARKKLKASLRTYG
                     KEYGFSVDE"
     assembly_gap    4473..4521
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4706..5116
                     /locus_tag="PK736_04185"
     CDS             4706..5116
                     /locus_tag="PK736_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRC32622.1"
                     /translation="MRGSISGRFTFESSFQMMNPKWSYEKNLAVFGKTNVDEPCLHTY
                     QLMVTVSGELDLETGFVVDLKLLRSLINKEVIQKMNHKNLNKDMNEFKGYVPTCEIIA
                     YVIWTMIRSKIHPRHEIKITIQEDENTIATYGEA"
     gene            complement(5133..5882)
                     /locus_tag="PK736_04190"
     CDS             complement(5133..5882)
                     /locus_tag="PK736_04190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC32623.1"
                     /translation="MKISIITITFNSADTVEETIKSVLAQDYPELEYIIVDGLSTDNT
                     MEIVKKYSDKIDTIISEKDKGLYDALNKGIKASTGDIIGILHSDDIYNGNDVISGVAK
                     HFTLWKCDAVYANLLFVERNDINKIKRVWRSGSYYDGAFRRGWMPPHPTFFVYKKYFE
                     TIGYYRTDLRFSGDYELMLRFIHKNKLKISYYDNFIIKMRMGGISNTNFWHKWLAHKE
                     DVKAWKYNQIVASTFWLWLKPISKILQYIKR"
     gene            complement(5953..6504)
                     /locus_tag="PK736_04195"
     CDS             complement(5953..6504)
                     /locus_tag="PK736_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WcaF family extracellular polysaccharide
                     biosynthesis acetyltransferase"
                     /protein_id="HRC32624.1"
                     /translation="MQADLSKYNNSWYKPGANAFVRFCWYFVNILFFINPLNPFSGVK
                     VFLLRMFGAKLGKGVIIKPSVNIKYPWHLSVGNYVWIGEHAWIDNLTKITIGNNVCIS
                     QGAMLLTGNHNYKKPTFDLVVRTIILEDGSWIGAHAIVCPGVVCGQNSILAVNSVATH
                     NMDENMIYQGNPAVSVRQRIIAT"
     gene            complement(6507..7676)
                     /locus_tag="PK736_04200"
     CDS             complement(6507..7676)
                     /locus_tag="PK736_04200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC32625.1"
                     /translation="MPDKPIILVAIDWYLPAFKGGGPIQSVANIISHLGHLYQFRIIT
                     GDRDLRDKKPFDNITFDKVILAQDNTATCYISKPNQTYVFLQNLISHSDANILYLNSM
                     FSVKFSLLPLLISKRIKKNMSVIIAPRGMLADGALSIKPIKKKIFLQLAKSFGWYRNV
                     IWHASTELESTEIKKIFGDTAIIKVARNLSHKQNIIAQPKQKEKGTLRLAFLSRIARK
                     KNLLAVFDYMQNTNPQAKIIFDIFGNIDEPDYWQQCETKIKNLPSHVQCNYKGEVPQH
                     LINTTLLQYDFTILPTLNENYGHAIVESWSAGCPVILSDNTPWRNLEQQNLGWDINLS
                     RPEQFTRAIDKCVDIDNNEYNKMSNACFIFAQQVVNNNDAVQQNIELFNIALNKK"
     gene            complement(7666..9537)
                     /gene="asnB"
                     /locus_tag="PK736_04205"
     CDS             complement(7666..9537)
                     /gene="asnB"
                     /locus_tag="PK736_04205"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRC32626.1"
                     /translation="MCGIAGIYLSTATKPDSASLQKMNNSIAHRGPDAEGYYSGPHIG
                     LAHRRLSIIDLSDAGTQPMHSGDNRYHIIFNGEIYNFEEVKSKLPSYPFKTKTDTEVI
                     LAAYIAWGKDCVHHFNGMFALAIWDSTEQSLFIVRDRLGVKPLYYHRNGEHFIFASET
                     RALIASGMYKPVINKTHLHEYFLYQTVHAPNTLLQEVMMLMPGHYIFIKNGDIKIEAY
                     WKTEKNISNLASSQTYDEVKQSVNKLLHDAVERRLVSDVPFGAFLSGGIDSSAVVGIM
                     SKYLQQPVRTFNISFDESEFSEAKYAQIIAKKFSTQHTELKLSPQHFINDLPLALDAM
                     DHPSGDGPNSYTVSKVTKAAGIKMALSGLGGDEMFAGYPVFNRTLKLQKNNLWWSVPA
                     LLRKLPASIVKNIKHDVPSQKLAALLAANEFNQAEFFALSRQLNVPVRIQSLLHTAAP
                     EKDVVENLVSTYFNNANIPMLSRVSMAEINTYMQNVLLRDTDQMSMAVALEVRVPFLD
                     YQLCEYVMGIPDKYKQPDYPKKLLVESLGDLLPPEIVHRKKMGFVFPWEHWLKNELRS
                     FCDERIQSLANRSFMKGENLIEQWKKFLHNDNSIRWIDIWICVVLEHWIVKNNIDAR"
     gene            9697..11016
                     /locus_tag="PK736_04210"
     CDS             9697..11016
                     /locus_tag="PK736_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32627.1"
                     /translation="MLIFLAGVCWHYAFDFYVTQTGFVNDVFRLNFENNVMPFYGRVK
                     LVANNNFFLIGSAIVLSFCIYRYKAFLFSNEKYISNYVLFIYFILIILFAITESAERV
                     SAVNVHYNSFANDLHYYSSVSDILKNYVSKQSIMDIHNQHYPPGNLIVLKIFENVGLL
                     PFAKWFFVTISLCLISYCCYLLKVDYGNAYTNLFSLLLCTCPGILIFPSIDNSLWYAA
                     LAFLSFLSFVLYLKKFSLHHLFVCILSLVIFAFFSFKIILITVAIAIYFLAHILNNNK
                     TEIQKYFIVPVYILAGFGMVYFALYLFSGFNLLDCILNSSHLNMKVMQTNGWDNVWRY
                     LLRSTGNYFGLFIGAGIVLLFLFCKPNISDKSRNIFFATNATILFGCFSGLFFMETER
                     VFVCLIPLIVISAVFSIENISAKQKAIIVIASLTFSVLQELFLQHYL"
     gene            complement(11021..11716)
                     /locus_tag="PK736_04215"
     CDS             complement(11021..11716)
                     /locus_tag="PK736_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC32628.1"
                     /translation="MGKTLKKILLVEDDFNFGNVLKNYLELNDYNVTLSRDGSEGIDA
                     FYKHKYDICLLDVMMPKKDGFTLAKEIRQQNANVPIIFLTAKSMKEDMVQGFQSGADD
                     FITKPFDSDVLLLKIKVLLKRASESFIMNEENDFEIGKYHFDYKSRNVTLDGVSNQLS
                     PKEAELLKMLCIHTNDIMPREKALKAIWGDDNYFTGRSMDVFITKLRKYLKEDPRVDI
                     LNIHGKGFRLQIQ"
     gene            complement(11757..>12034)
                     /locus_tag="PK736_04220"
     CDS             complement(11757..>12034)
                     /locus_tag="PK736_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC32629.1"
                     /translation="DALAICVSDNGIGMNEETQKRIFETFYRAPTGNIHNVKGFGLGL
                     SYVKAIIDDMKGEIEVKSEPGKGSTFIVTLPLLIPKKTDKEQELINA"
BASE COUNT         3502 a         2145 c         2259 g         4079 t
ORIGIN      
        1 tgcaatttgc gtcaacaaca aaataacaaa aagagcacaa atatttttca ttttgaaaaa
       61 ttcaacaaca tgataagata attcaaaata tggaatgttg aacaatggaa tatgcgcttg
      121 agataataat ttgtgtcatg aatcttttca cacctcatta acaaaacact ttagtttatt
      181 tgtaatgtta aaattgcata tttgcagcgc ataagcaaaa catttttttt cgattttcat
      241 tgagccacaa cttagtcatc atccctacct ataacgagtg cgaaaatgcc gaaagcatta
      301 tccgtgctgt attggcgctc gctacaccct ttcacatact gatagtggac gataactcgc
      361 aagatggtac ggctaccatt gtaaagaatt tgcaaaagga atatgaaggt cgtttgcaca
      421 ttgaggaacg tgcgggcaag ttgggtttgg gtactgctta catccatggt tttaaatggg
      481 ccttgcaacg caactacgat tatatttttg aaatggatgc cgatttttcg cacaaacctt
      541 ccgacttgct caagctgcat aaggcctgtg ccgaagaagg tgccgacctc gcaatcgggt
      601 cgcgttacag caatggtgtt actgtggtga attggcccat tggcagggta ataatgtcgt
      661 attatgcttc ggtatatgta cgtacaattt tgggtgtgcc catacacgat accactgcgg
      721 gttttatctg ctacaaaaaa agggtattgc aaatgataaa ccttgataag ataaaattta
      781 ccggctatgc atttcaaata gaaatgaaat tcacggcttt caaacacgga ttcaaaatag
      841 tggaagtgcc catcattttt atggatcgta aacttggaaa atcgaaaatg agtagtggca
      901 tttttagcga agcttttttt ggagtgatag agctaaaaat aaaaagctgg tttcgcaagt
      961 acgagcaagc gcgggatagt ttttagtttc ttagtttata gttcttagtt acgtttgctg
     1021 tgagcctttt tcttagtccc gacactacgg tcgggatgcc gaccgaagtg tcggcatttt
     1081 tagtttcgtt tgctgcgagc cttgtgctta gtttttttgt tcttaattcg aaattagatt
     1141 tttgactttt catttgaact tacatttaac taaaaactaa aaactttccc ctttgaactt
     1201 accccattaa ccttctccct tttgccttct taaaattatt gcttcacaaa tttggtgttg
     1261 tacaatttgt tgttggcaaa tagctgcgcg ttatacattc cgggtgctaa atcattaatg
     1321 tcaatatttg ctgagccaga tacagccgaa atagttttta ccgttttgcc taacatatcc
     1381 tgtatacgta cttcgtagtt ttgctgccag cccggtatta cggtgatgct atttgttgcc
     1441 ggattgggtg ctacactgta ttgttgttgc atccatgctt cggtgccacc cagcgagaag
     1501 ttgcatccat tgtacgattg caccgtcata ttggtatcta ccacaatgtt ttcatcgccc
     1561 ggtaaataat aagtgtagaa aaaataaatc agcatcattt cgttggtggt attttcgcca
     1621 aggtaaacgg ccttgggtgg agtgttggga ttgtttagat tgttggcagt gttatcataa
     1681 aaggcgcgcg aatgcaaagt ggtaccgctg ggcactttca ccggttgacg aaaatcgtaa
     1741 gcaccctgcc agtgaaaatc ccatttagga atatcaatta atttcaaagt atcgttgagc
     1801 ggggtaacac cacgtgcgct gatctctgtg cccacaagat gcatgtgtgg cgccaccgaa
     1861 agcacagtca catcaatagg cacagtataa tcacaagtaa aagttcttac actatcggca
     1921 ggtatgaata aaaacccttc gttaagatca tcgtgattca aaataggatc gatgtttacc
     1981 tgacgggtaa atggtgtggt agaatatttg aaattaacct gtgtgctatc gtattgcccc
     2041 gaagttccaa gcggataatg tatttgaaga atgatatgag aattagcttt caacttaatg
     2101 cccattgcct gtggcagaaa gtaaggctga gcgcccggtg cccacagtcc aataagttcg
     2161 gcagtattgc tgcctatgcc tccaaaactt gtgtagccgg gtaatgtatc ggcattatcg
     2221 agattgataa ctgtattgcc ctgatcctga aaaaccaaca catgatgcac tatatttctg
     2281 ttgcccggca acacttccat gcccgaaata aatttatcga tacttgttac gtttgggata
     2341 acaaagcagc gatacaaatc ggatgttata ggtggtattt gataggttgg cattttcgta
     2401 ttcaaatcag gattgggcat ttgcgatatt cctgaatata ccggtggagt aggtgcattg
     2461 gccgcattac cttgtggcga accggctgca acccattgat caatgatatc aatttcgtct
     2521 tgtgttagca cgcgctcatg tgcatatgtt tgataactat ggttgggtgg ccagggtggc
     2581 atacgcttgg cttgcaccga tgagtcgatg tcttctgcaa agttgaatgc atcagtgtat
     2641 gtcattaatg aaaacggtgc tgccccatta gggttatgac aactggtgca gcgtgtgtat
     2701 aaaatgcatg caacattatc ggcccatgtt ggtgttacct gtgcttgcat ttgaaatgcg
     2761 cttatcgcaa aagcgaatgc agtaattatt tttatttgtt tcatattgtt tatgcagtat
     2821 tagtttttta gataatctaa accggaatta aatttatgct ttgcaaatat attgttttga
     2881 tagtttaatg caagttggtt taattaaaag tttctttact aaaatattag aggttgcaat
     2941 taatttctaa gtcttaatat aaatgtgacg agtagtgtta agcaaggtta caagcacgaa
     3001 gaatgaagtt acacaaagaa agaagtacgt aaaaaaactt tgtgaacctt ttgtggaata
     3061 atttattgat gaaagaaagt tgtcacacaa agtaagaatg aagtcggaca gagattttcc
     3121 ctgatattat tttttcaatt gagttgcaag ccacaaaaat tcgcgcaaaa ataagtctat
     3181 gcttttttga aaattggggt cgattaatgt gccttgctca tcgaattttt tatccactgc
     3241 aggaatcacc aacatgtagg gagataaaat tccaaacaat gccggtacca attgttgcat
     3301 ttgcattgct gcgcgtatac cacccaatcc tcctggcgat gctgttacaa taccaaatgc
     3361 ttttcgcgat tgcttaggaa aatggtcgaa cacatttttc aatgagggtg tatacgaacc
     3421 gttgtactcg ggagttaaca tgattactgc atcggcttca aacatcagat ttgcccattg
     3481 tttgtgttca tcgggtaccg cttctttatt tgcaaatcca tcagtaataa atggcagatt
     3541 tatttcgcgc acatcaataa tatcgctatg cagttttaat tgtgtaagct gattgtgtaa
     3601 acataaggca acgcggttag taacactttc gtgccgcatg cttcccgaaa cgataactat
     3661 tcgcataatt gaactttaat ttattgtagt gcaaacctaa cagtaaaaga gtaacaaagt
     3721 ttgctgtgcc gggattaatg ttttttaaaa atggcagaca tggaaattat taaaatcgaa
     3781 tcattatgtt gtgaaacata gattagataa taatattaag aaatgataaa atagtgtcaa
     3841 tgataagata gccggatagc atatgtgtat gccattaccg gaaatgcctg aggcttggca
     3901 aaaaaaaata gtaaaaatga ttcttactat tttattgtta ccactcgaaa tgaagttcaa
     3961 ctattcatca accgaaaagc cgtattcttt accataagtg cgaagacttg cttttagttt
     4021 tttgcgcgca cagtgaatgc gaatttttac tgttccaagc ggcaatgaaa gatagtttga
     4081 tatttcatcg tatttgtaac cggtaaaact cattaaaaat ggaattttta aattgtcggc
     4141 aagcaaacct atagcattat ttatatcctt taatgcaagt ttactagtgg cctcgttgcc
     4201 cgtggtgaat gatgtattgt ttatgtagta ttgattatcg gtatcatcgt gagtgatgtt
     4261 acttttctta ttgcgtctgt aattattaat gaaaacattt ttcataatgg tatacatcca
     4321 ccctttgagg ttggtgccgt cctgaaattt gtcctgatta gcaatcgctt taagcatggt
     4381 gtcttgcgtt aagtcttcag catcttccat gttttgcgta agcttaagtg catatgattt
     4441 aagctttgaa taggaccctt gaacttgttg atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nttgattgaa actttccttt tccatttgtt tatgttttta
     4561 ttataaatat tgcacaaatg aactgctaaa taaattaact tccaaatttt tgtttaataa
     4621 aaagtgttaa atgttgaaca aaaaatttta catactttaa acttaattgt gaaggcattt
     4681 gttagaactt taaataaact aacgaatgcg gggtagcata agcgggcgat ttacatttga
     4741 gtcgagtttt caaatgatga acccaaaatg gagttacgag aaaaatcttg cagtatttgg
     4801 caaaaccaat gttgacgagc catgcctgca tacctatcaa cttatggtaa cggtgagtgg
     4861 tgaacttgac cttgaaaccg gttttgttgt agatttaaaa ttgttgcgct ctctcattaa
     4921 taaggaagtg atacaaaaaa tgaatcacaa aaacctaaac aaggatatga acgaatttaa
     4981 gggttatgtg ccaacgtgtg aaataatagc ttatgtaatt tggactatga ttagaagcaa
     5041 aatacatccc cgtcacgaaa taaaaataac cattcaagaa gacgaaaaca ctattgcaac
     5101 ctatggcgaa gcatagatta tctcgctgaa aattaccttt ttatgtattg aagaattttt
     5161 gaaataggtt taagccataa ccaaaatgtg cttgctacta tttgattgta tttccatgcc
     5221 ttaacatctt ctttatgagc aagccatttg tgccaaaaat ttgtattgct aataccgccc
     5281 atgcgcatct taataataaa gttatcatag taacttatct taagtttatt tttatgaata
     5341 aaacgcaaca taagttcgta atcgcccgaa aaacgtaggt cggtgcggta gtatcctatt
     5401 gtttcaaaat actttttata aacaaaaaaa gtaggatgtg gtggcatcca acctctgcgg
     5461 aaagcaccat cgtaatatga acctgagcgc catacccttt ttattttatt tatatcgttt
     5521 cgctccacaa aaagtaagtt ggcatatact gcatcgcact tccatagggt aaagtgtttg
     5581 gcaacacccg aaataacatc gttgccatta taaatatcgt cagaatgaag aatgcctata
     5641 atatcgcccg tggatgcttt tattccttta ttgagtgcat cataaaggcc tttgtctttt
     5701 tcgctgatga tggtgtcaat tttgtcggaa tactttttta ctatttccat agtgttatcc
     5761 gttgacaagc catcaacaat aatgtactcc aactcgggat aatcttgtgc taaaactgat
     5821 tttatagttt cctcaacagt gtcggcacta ttaaaagtta tagtaataat agatattttc
     5881 ataaaagcta tttgcccctt aaatggttca caaagttata atttcaatca attatactac
     5941 caaacttatt ttttatgttg caataattct ttggcggaca gaaactgcag ggttgccctg
     6001 ataaatcata ttttcgtcca tattatgcgt tgctactgag tttacagcga gtatagaatt
     6061 ttggccacat actacaccgg ggcaaacaat agcatgcgca cctatccaac taccatcttc
     6121 caaaataatg gtcctcacta ctaaatcgaa tgtaggtttt ttataattat gattgcctgt
     6181 aagcaacatg gcaccttgcg atatacaaac attattgcca atggtaattt tggtaaggtt
     6241 atcaatccac gcgtgttctc ctatccaaac gtaattgcca acagaaagat gccagggata
     6301 tttaatattt accgaaggtt taataataac gcccttgcca agtttagcgc caaacatgcg
     6361 gagcaaaaac actttaacgc cactaaaagg atttagaggg tttataaaaa aaagtatgtt
     6421 tacaaaatac cagcaaaagc gtacaaaagc atttgctccg ggcttgtacc aactgttatt
     6481 gtattttgat aaatctgcct gcatatttat tttttattga gagcgatatt aaataattcg
     6541 atgttttgtt gcacggcatc attattatta accacctgct gcgcaaatat aaagcatgca
     6601 ttactcattt tattgtattc attgttatct atatcaacac atttatcaat tgcccgtgtg
     6661 aattgttcgg gcctactcaa gttaatatcc catcctaaat tttgttgttc aagatttcgc
     6721 cagggtgtat tatcgcttaa aataacagga cagccggcac tccagctttc aactattgca
     6781 tggccatagt tttcgtttaa cgtaggtaaa attgtaaaat catattgtaa caaagtggta
     6841 ttgataagat gctgtggtac ttctcctttg taattgcatt gtacatgaga aggcaggttt
     6901 tttatttttg tttcacactg ttgccaataa tccggttcgt caatattgcc aaaaatatca
     6961 aatataattt tagcctgggg gtttgtattt tgcatgtaat cgaacacggc caacaggttt
     7021 tttttgcgcg caatacgcga taaaaatgct aatcgcaatg tgcctttttc tttttgcttt
     7081 ggttgagcaa taatattttg cttatggcta aggttgcgcg ctacttttat aattgccgtg
     7141 tcgccaaata ttttttttat ttcggtactt tcaagctcgg tagatgcatg ccatataaca
     7201 ttgcggtacc agccaaagct ttttgctaac tgtagaaata tttttttttt gattggttta
     7261 atactcaaag cgccatcggc aagcatgcca cgtggtgcaa taattaccga catatttttt
     7321 tttatccttt tcgaaataag aagaggcaac aaactaaact ttaccgaaaa catgctattg
     7381 aggtatagta tattggcatc gctatgagaa attaagtttt gcaaaaaaac atatgtttga
     7441 ttgggtttac tgatataaca ggtggctgta ttatcctgtg caagtatcac tttgtcgaag
     7501 gtaatattat cgaaaggttt tttatcgcgg aggtcgcggt caccggtaat aatcctgaat
     7561 tggtataaat gtcccagatg gcttataata ttggcaacag attgaatagg acctccgccc
     7621 ttaaaagccg gcagatacca gtcaattgcc acaagtataa tcggtttatc gggcatcgat
     7681 attgtttttt acaatccagt gttcaagtac tacacaaatc caaatatcta tccagcgtat
     7741 actgttatcg ttgtgtaaaa attttttcca ttgttcaatt aaattttcac ctttcataaa
     7801 agatcggttg gcaagcgatt gaatacgttc gtcacaaaaa ctgcgaagtt catttttgag
     7861 ccagtgttcc catggaaata caaagcccat ctttttgcgg tgtacaattt caggtggcaa
     7921 taaatcgcca aggctttcga caagtaattt ttttggatag tcgggttgtt tgtatttatc
     7981 gggaatgccc attacatatt cacacaactg ataatcgaga aaaggtacac gcacttcaag
     8041 tgccacggcc atgctcattt ggtcggtatc gcgcaacaac acattttgca tgtatgtatt
     8101 tatttcggcc atgctcacac gactcaacat tggaatattg gcgttattga aataggttga
     8161 taccagattt tcgacaacat ctttttcggg ggcagcagtg tgtaacaatg actgtatacg
     8221 cacaggtaca ttaagctggc gcgaaagagc aaaaaattct gcctgattaa attcattagc
     8281 agctaacaat gccgcaagtt tttgtgatgg cacatcgtgc tttatattct taacaatgct
     8341 tgcaggtaat tttcttaaca atgcaggaac actccaccac agattatttt tttgtaattt
     8401 taatgtccgg ttaaataccg gatagccggc aaacatttca tcgccaccca agcccgacag
     8461 tgccattttt attcctgctg ctttagtaac tttcgatact gtgtatgagt ttggtccatc
     8521 gccactggga tggtccatag catcgagcgc aagtggcaag tcattaataa aatgttgcgg
     8581 acttaatttt aattcggtgt gttgagtgct gaatttcttg gcaataattt gtgcatactt
     8641 tgcttcgcta aattccgatt catcgaaact gatattgaag gtgcgaacag gttgttgcaa
     8701 atatttactc ataatgccaa ctacagcact ggagtctata ccgcccgaaa ggaaagcgcc
     8761 aaaaggtaca tcgctcacaa gtctgcgttc aacagcatcg tgcaataatt tatttacact
     8821 ctgttttact tcatcgtaag tttgtgagga agcaagattc gaaatatttt tttcggtttt
     8881 ccagtatgct tcaattttta tatcaccgtt ttttataaaa atataatggc ccggcataag
     8941 catcattact tcttgcaata aggtatttgg tgcgtgtaca gtttggtaca aaaaatattc
     9001 gtgcaaatgt gttttattaa taacgggctt gtacataccc gatgcaatta atgccctcgt
     9061 ctccgatgcg aaaataaaat gctcaccatt tctatggtaa tataatggtt ttacacctag
     9121 gcggtcgcgc acaataaata aactttgttc tgtactatcc catattgcaa gtgcaaacat
     9181 tccgttgaag tgatgcacac aatctttacc ccatgctatg tatgcagcaa gaattacttc
     9241 ggtatcggtt tttgttttga aagggtagga gggcagtttg ctttttactt cttcgaaatt
     9301 ataaatctcg ccattgaaaa taatatggta gcggttgtcg ccactgtgca taggctgagt
     9361 accggcatcg ctcaaatcga ttatggaaag tctgcgatgt gccagaccaa tgtgtgggcc
     9421 cgagtaatag ccttcggcat cgggcccgcg atgggcaatg ctattgttca ttttttgtaa
     9481 tgatgcactg tcgggctttg ttgccgtgct caaatatatt cctgctatgc cacacataat
     9541 tggggcaaaa ttagttttaa atttgtacaa aagttcttat cagaaataaa tgtgtgttaa
     9601 catgggtaaa aatcatttct tatgtttacc gtttcgtaag gaagtatgac agagaaatta
     9661 ttttcacctt tattttcacg gtgcattctt ttaggaatgc tcattttttt ggcaggcgtt
     9721 tgctggcatt atgctttcga tttttatgtt acccaaacag gttttgtaaa cgatgtgttt
     9781 cgcttaaatt tcgaaaacaa tgttatgccc ttttatggca gggtgaaatt ggttgcaaat
     9841 aataattttt tcctaattgg atctgcaata gtgttaagct tttgtattta ccggtacaaa
     9901 gcgtttttgt tctcaaatga aaaatacatt tccaattatg tgctttttat ttactttatt
     9961 ctgattatat tatttgctat aacggaaagt gctgagcgcg tatctgcagt gaatgtgcat
    10021 tataattctt ttgccaacga tttgcattat tattcttcgg tgagcgatat tttaaaaaat
    10081 tatgtaagca aacaaagtat aatggatatt cacaaccaac attatccacc gggaaattta
    10141 attgtactca aaatttttga aaatgttggt ttacttcctt ttgcaaaatg gttttttgta
    10201 acaatcagtt tatgtttgat ttcgtattgt tgttatttat tgaaagtgga ttacggcaat
    10261 gcctacacca atttattttc attgctttta tgtacatgcc cgggcattct tatttttcct
    10321 tcgatcgaca attcgttgtg gtatgcagct ttggcatttt tatcttttct ctctttcgtt
    10381 ttatatctaa aaaagttttc actccatcat ttatttgttt gtattttatc gttggtgatt
    10441 tttgcctttt ttagtttcaa aattatttta ataactgttg ctattgccat ttattttctt
    10501 gctcatattt taaataacaa caaaacagaa atacaaaaat attttattgt tcctgtttat
    10561 atacttgcag gttttggaat ggtatatttt gccttgtatc ttttttcggg atttaatttg
    10621 cttgattgca tactcaattc atcgcacctc aatatgaaag taatgcaaac aaacggatgg
    10681 gataatgtgt ggcggtattt attgcgcagc acaggaaatt attttggatt gtttattggt
    10741 gcgggtatag tcttactgtt tttattttgc aagccaaaca ttagtgataa atcacgcaat
    10801 attttttttg ctacaaatgc caccatttta tttggatgtt tttcgggctt gttctttatg
    10861 gaaaccgaaa gggtgttcgt ttgtttgatt ccattaattg taatcagtgc tgtttttagc
    10921 atcgaaaata tttcagcaaa gcaaaaagca attatagtta ttgcgtcatt aacctttagt
    10981 gtgctgcaag aattattctt gcagcactat ttgtaatttt ttattgaatc tgtaaacgga
    11041 aacctttacc gtgaatattc aatatatcga cccgaggatc ttctttcaaa tatttgcgta
    11101 gcttagtaat aaaaacatcc atgctgcggc cggtaaaata attatcatcg ccccatattg
    11161 ctttcaacgc tttttcgcgt ggcataatat cgttggtatg tatgcacaac atttttaaca
    11221 actcggcttc ttttggcgac agttgattac tcacaccatc caaagtcaca ttgcgcgatt
    11281 tgtaatcgaa atggtatttg ccaatttcaa aatcgttttc ttcgttcatg ataaaacttt
    11341 cgctggcacg tttcaacaac acttttattt tcaataacaa aacatcacta tcgaagggtt
    11401 ttgtgataaa gtcatcggca ccgctttgaa agccctgcac catatcttct ttcatcgatt
    11461 ttgcagtaag aaaaatgatg ggcacattgg cattttgttg ccttatttct ttggcaagtg
    11521 taaagccgtc tttctttggc atcattacat caagcaaaca tatatcatac ttatgtttgt
    11581 agaatgcatc tattccttcc gagccatcgc gcgaaagtgt aacgttatag tcgtttaatt
    11641 caagataatt ttttagcaca ttgccgaagt taaaatcgtc ttcaacaagc agaatttttt
    11701 tcaatgtctt tcccataata ttttatgttt ttaaatgtgt tttttgttat tgatttttat
    11761 gcatttataa gttcctgttc tttgtcagtt ttcttgggaa taagcaatgg tagcgttaca
    11821 ataaaggtgc tgcctttgcc gggttcactt tttacttcta tttcaccttt catatcatcg
    11881 attattgctt ttacataact aagccctaag ccaaatcctt ttacattgtg aatattacct
    11941 gtaggtgcac ggtaaaatgt ttcgaatatt cttttttgag tttcttcatt catgcctatt
    12001 ccattatcgc ttacacaaat agccaaagca tcat
//
LOCUS       DAORQA010000039        11936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1448_length_11936_cov_11.915159, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000039 DAORQA010000000
VERSION     DAORQA010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11936
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1448_length_11936_cov_11.915159"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            403..1128
                     /locus_tag="PK736_04225"
     CDS             403..1128
                     /locus_tag="PK736_04225"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRC32630.1"
                     /translation="MTEQKKPRSIFLTLFYHALPAFFFVALMWLMYFIQLINFTDYNL
                     SNLGVWPRHKSGMYGILFSPFLHGSFDHLISNSGPILILGTMLIMFYRAIWFRSFVML
                     YLLAGIGLWMGGRDNIHIGASGIVYSLAAFLITIGLLRLEIKLVAISFLVIFLYGGIV
                     WGILPILEHVSWEGHLFGMMSGIITAFYYKDEGREKPKLYQWEMEEIDALMEQNKIAD
                     AEKLLKDENDFPQFKYHYKPKEE"
     gene            1186..1593
                     /gene="mce"
                     /locus_tag="PK736_04230"
     CDS             1186..1593
                     /gene="mce"
                     /locus_tag="PK736_04230"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRC32631.1"
                     /translation="MKKIEHIGIAVKNIDDANSIYEKLLGTSSYKTEEVLSENVITSF
                     FKCGESKIELLQATNENSAIAKFIDKRGEGIHHIAFEVDDIHAEMKRLHNEGFVLLNE
                     QPKQGADNKLICFVHPKNTNGVLIELCQEMKQV"
     gene            1645..3096
                     /locus_tag="PK736_04235"
     CDS             1645..3096
                     /locus_tag="PK736_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRC32632.1"
                     /translation="MMNRYILTFFALAINISLCIAQPLATKMDEAMKKYTAQNYSGNV
                     LVYSNGEKIYDKAFGFADRENKRPQTTETFFSIGSITKQFTAAGILLLEQQGKLSVND
                     KMSKYYDNVPSDKADITLHKLLTHTAGFTETLGDDYDTINAAQFATLALASKLKQNAG
                     TNYLYSNVGYSMLGIIIEKVSGMGYEKFLHDNIFVPCKMNNTGYCIPLFDRAKFAIGY
                     RDNVRWGSAISKPWAADGPGWHLRANGGIISTTNDMLNWYLALASNKILNKAETEKYF
                     TPHVAEGHQQNSFYGYGWVIQNAGDEKWIWHNGGNGVYNANVTFNLEKNWCVIVSSNS
                     NNIIADDIALDMIGVLKNKTMTHAPQQNTSPDVNPVSQKIVDVISSRGAEYYSQHYND
                     ILKETGFDFKNDMILLDAGQLLEDKKMWDGGISLYESCTKLFPNIIIGWNRLGYCHLQ
                     KGDKEKARTCWKKSLTIRTENNRAGEFLKSINN"
     gene            complement(3093..3839)
                     /gene="phbB"
                     /locus_tag="PK736_04240"
     CDS             complement(3093..3839)
                     /gene="phbB"
                     /locus_tag="PK736_04240"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HRC32633.1"
                     /translation="MDKQSKRIVLVTGATGGLGTAMCKELYTDGFHVIANYRNKQKAD
                     EWQAKINAEGFVIDMYEGDVTDYDSVGAMIAKIEAEVGPIDTLVNNAGITRDIRFSKM
                     SKEDWDAVIATNLTSVFNCTRHVINGMMERKYGRIINISSVNGQRGQFGQANYSAAKA
                     GMHGFTKTLAMEVAKYGVTVNTISPGYIGTDMVMAVAEKVREQIVAQIPMGRLGGTEE
                     VAHLVSFLASEKTNFITGANYSINGGQHVY"
     gene            complement(3883..4392)
                     /locus_tag="PK736_04245"
     CDS             complement(3883..4392)
                     /locus_tag="PK736_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HRC32634.1"
                     /translation="MMNLVYIALAALVPMIVGFIYYNPKTLGTAWMNASGMTEEKIKS
                     GNMGVIFGVSFLFSFMLAFVLQTMVIHQYNVMSTLVDEPGFNDPNSAMGTYYTDFMTK
                     YGQNFRTFKHGMLHGFILSLFFVMPVMGVNALFERKSFKYIFINVGFWAISLMLMGGI
                     ICQFIKFPD"
     gene            complement(4477..6816)
                     /locus_tag="PK736_04250"
     CDS             complement(4477..6816)
                     /locus_tag="PK736_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC32635.1"
                     /translation="MVQNIKGKVIDAETKTPLFGATIVIADDSTSNTMGGVTDYDGNY
                     KIAKVPVGRHRIAIRCLGYKETVLSNIVVSSAKEMVLNVELQARIMDIKEITVSAGSK
                     ADVINEMATVSARTFTVEETQRYAGSRDDAARMASNFAGVQGGDDSRNDIVIRGNSPW
                     GVLWKLDGVDIVNPNHFAVPGTTGSAINLLNSKTFANSDFMTGAFPAEYGNAIAGVFD
                     LRLRNGNNEHHEFTAQLGFLGTELLAEGPISRTNGSSYLATYRYSTFQVFSALSIPIG
                     TSAVPKYQDGTIKINLPTKRAGNFTIFGVGGKSDIEILVSDKTEPTEEIYGDKDRDQY
                     FGSSTFVGGVSHTFPLGKNTYMKTVLSHQLAGAHAYHELVIRNPDFSVDTLLPKLNYK
                     FATQKTSLAWFINTRIKLQHIIKAGINVDRFNFNMADSNYLEKINVWDNRLDYNGDAF
                     LVQPYIQYKYKITDVLSINGGIHAQLLTINENSKAIEPRVGINWQVSEKSTVSAGYGM
                     HSQMLPDYIYFAHRKDSAGKYNMLNNDLKFFNSQHIVLGYDYSLNKYSRIKIETYYQS
                     LSNIPVEIKNSSYSILNQGSSFSRIFPDSLVNAGAGENYGIEFTLERFFNRNFFFLFT
                     SSLYNSTYKGSDGEKRNTDFNGNFIVNALAAREYKLGKKNRTTLTTGAKVTWAGGQRY
                     TPADVHASNLAGELVVIDSLRNVKKFKDYFRLDFKLGIKVNTKKFTHEIALDLVNILN
                     TKNVLGLTYVPVDNASNQSPLREEYQLGFLPLFYYKIDF"
     gene            complement(6935..7378)
                     /locus_tag="PK736_04255"
     CDS             complement(6935..7378)
                     /locus_tag="PK736_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRC32636.1"
                     /translation="MNSTFKIFIVAFVALSTLIYAGFKAENNALKEISACMQSQQASW
                     NSGNLEAYMGCYWKSDSLQFVGKRGPQYGWQQTLSNYKKSYPNAEAMGKLQFTNLKMT
                     PLCEQYVYVFGKWELLRTKDTLSGYYTLIWQYIDGRWVIISDHSS"
     gene            complement(7615..>11936)
                     /locus_tag="PK736_04260"
     CDS             complement(7615..>11936)
                     /locus_tag="PK736_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC32637.1"
                     /translation="PQSIGALVVSFCLLTFCSSITLFGQTYKYKSYNESPGLPSYVSN
                     ELAQDANGRLWVSTSNGLAYFDGTEWTTVKHIADTIDAREYEHLEHDKNGNIYFFPKN
                     DNIDIIKYDGKIYSSIKISQPDTCAFAAISKCFAATINGKMVIAYSTATSFRVNYQNK
                     WYTYNKTNGYAGERILNIAKYKSSVVFCTEARLYEWSNGKLQFNINAIIPPSFLPVRG
                     MTDEPSTTKGNTTTWMFGNTWVGYIENDRFTCINNSLNFIQNYDSGDDLILGDGNNRV
                     FISTHLSCYYLEKSTKKIFNFSLRDQETITWFTDMIFDRENNLWFTSQRGVGKINSFN
                     FVNYFPPVNNNDNEVSSIIQLENEKFFFGSKYGYYIFNNGKITTHEFTSVGNQPLSSN
                     RIGDAIKDNKGQVYFIAGQLGICKLMPNEGYSVVIPFSNYPEEITSLTTDAQGTAIAV
                     GSSSMYKLINGKLIFLEEIFTSQQYVRKLFFGKAGEYYIATTQGVFCRQNGKTQPLLF
                     ENKSDNVSAYCLVDDGSGNIIIGNTLGLFVYHNNKATRFKHHEFLNTLPVYFIQKDNK
                     NNLWIGTNDGVWKLNRYHAWPYSTENGLSGRETNRSAICLDNKGRLWIGTNAGVSCYD
                     QEYNLNNLQKPIINLLNVENSIGETYGLEHDFAINYSNNSLTFHFNAISLTNEKRNQV
                     QVKLVGFDKDWFTLPKADLRNIRYTNLPSGNYFLKIRAKGDNGQWSDDVTSSQITINR
                     AFYTRWWFFILVFIAATSIIYPLLKYLQQKRNASVLQEMVNERTHNLQSSENRLRSIL
                     EHSNDGILITDKFGKIINANDAFCKLTGLTQQEITKSTVEKLSADFKMDVPLTMDRYT
                     EIFNEKEAHKGFESSAFINNEQRFLQINRVFIDAPAAGEVYMVNMFHDITDMKNTQQV
                     LIKSKEIAETATATKSAFLASMSHEIRTPLNGIIGLADLMQHTPLDDEQQEFINGLQQ
                     SGRTLLALINDILDFSKIESGKMELAEESVYLPQLIEEVFEIISSKAVEKQIDFTYYI
                     DEKVPTAIYSDSIRLKQILLNLLSNGIKFTDTGFVALNVKVNESGKDLQFMVRDSGIG
                     ISTENIGTLFNSFTQIKAHKGVSRGGTGLGLAISKRLASLMNGDISVASESGKGSVFT
                     LNMPCHESNDYSEFNKFIIDQKSLMQQLNNTPLFAMETQNVAIQNIMNEAKKYGINSQ
                     YISWQNFVSNIDSFVNQPIIIHQKHIESNEVISTWFAHNTKVLQGMMVLNFDARNQAN
                     NYTAYTDKYSLPLRYRAFFKYVSNKVSRLNDLSLFRAPVIQNTTQPAAPKYLIVDDVP
                     TNCMILSKLLANHGYQCDIAYNGVEAVTMATKTYYPVIFMDINMPEMDGWDASLAIRN
                     TEIEMRRRSTIIAITANALEQDKQKCFDSGMDFYFSKPFDFKKLIDLLQAIEEETKAK
                     INN"
BASE COUNT         3647 a         2261 c         2170 g         3858 t
ORIGIN      
        1 tttgcattta cttcattcac atccagttga attgtcttat aatatttgct ctgtgcaggt
       61 ttttttttct tcgattgagt ttgcgcattg atgtgaggcg aaaataaaat taaagccatc
      121 agcaaagaaa atagtgcacg aactattgta tatgatttat tttttttcac ttttattttt
      181 tcggttatga aaatggtatg caataatagc gcaatgtgtg tcaacaactg ccactttaac
      241 tattaatttg ctaacgtatt gctgttagca taatgattgc ttcattttac acctaacagt
      301 ttaaggattg tgacatttta tcgttgaaaa aaaaacatgt cataaatatt tcaactaata
      361 catgcaaaca agttaaatca aagaaagtaa atttgcaacg ctatgacaga gcaaaaaaaa
      421 ccaaggtcta tttttcttac actattttat catgcgttgc cggcattttt ttttgttgcg
      481 ctaatgtggc tcatgtattt tattcaacta ataaatttta ccgattataa cttgagtaac
      541 cttggagtgt ggccacgtca caagtcaggc atgtatggta ttttattttc tccctttttg
      601 catggcagct tcgatcatct tatttctaac agtggtccca tacttatatt aggcactatg
      661 cttatcatgt tttatcgtgc catatggttt cgctcctttg taatgttata cctgcttgca
      721 ggcattggtt tatggatggg aggtcgcgat aatattcata taggtgcaag tggcattgtg
      781 tattcattgg cggcatttct cattaccatt ggtttgctac gtttagaaat aaaattggta
      841 gccatatcgt ttcttgttat ttttctttac ggtggcatag tgtggggaat attgcctata
      901 ctggaacatg tttcgtggga ggggcattta tttggaatga tgtcaggcat tatcacagct
      961 ttttattata aagacgaagg gcgcgaaaaa ccaaaattgt atcaatggga aatggaagaa
     1021 atagatgcac tgatggaaca aaataaaatt gcggatgccg agaaattatt aaaggatgaa
     1081 aacgattttc cacaatttaa atatcattac aaaccaaaag aagaatagta aaaatttgca
     1141 agagagaact aacctcttca aaaataaaaa gaacaaaaaa gctaaatgaa aaaaatagaa
     1201 cacataggca tcgctgtaaa aaacattgat gatgcgaatt cgatatacga gaaattactt
     1261 ggcacatcat cttacaaaac cgaagaagta ttgagcgaaa atgtaattac atcttttttt
     1321 aaatgtggcg aaagcaaaat agaattatta caagcaacaa atgaaaatag tgccattgca
     1381 aaatttattg ataagcgtgg tgaaggcatt catcacatag cttttgaagt ggatgatata
     1441 catgccgaaa tgaaacgctt acataacgag ggttttgtgt tgctcaacga acagcctaag
     1501 caaggtgccg ataataaact tatttgcttt gtgcatccta aaaacaccaa tggcgtgctg
     1561 atagaattat gtcaggagat gaagcaagtt tagctttttg ttaactatac cactttcgtt
     1621 gttttttact tttgtaataa aaaaatgatg aacagataca tcctcacttt ctttgcatta
     1681 gcaattaata tttcgctgtg tatagctcag cctttggcta ctaaaatgga tgaggcaatg
     1741 aaaaaatata cggcacaaaa ttacagtggc aatgtgctgg tgtatagtaa tggcgaaaaa
     1801 atttatgaca aagcttttgg ttttgccgac cgcgaaaaca aacgcccgca aactacagaa
     1861 acatttttct ctatcgggtc aatcacaaaa caatttacgg cagccggtat tttgttgctc
     1921 gagcaacaag gcaaactgag tgtgaatgat aaaatgtcca aatactatga taatgttcct
     1981 tcggataaag ctgatattac cttacataaa ttactgacgc acactgcagg atttaccgaa
     2041 actcttggcg atgattacga caccataaat gcggcacaat ttgcaaccct tgcccttgct
     2101 tcaaaattaa aacaaaatgc cggcaccaac tatttgtata gcaatgttgg gtatagcatg
     2161 ctgggaatta taattgaaaa agtaagtggc atgggttacg aaaaattttt acacgataat
     2221 atttttgttc cgtgcaaaat gaataatacc ggttactgta tacctttgtt cgatcgtgca
     2281 aaatttgcca ttggctatcg cgacaatgta agatggggca gtgcaatcag caaaccttgg
     2341 gcagctgatg ggccaggttg gcatctgcgt gcaaacgggg gaattatttc taccacaaac
     2401 gatatgctca actggtatct tgcattggca tcaaacaaaa tattgaacaa ggccgaaacc
     2461 gaaaaatatt ttacacccca tgttgccgaa ggccaccagc aaaatagttt ttatggttat
     2521 ggttgggtta ttcaaaatgc aggtgacgaa aaatggattt ggcacaacgg tggcaacgga
     2581 gtttacaatg caaatgttac tttcaattta gagaaaaatt ggtgtgttat tgtatcgagc
     2641 aattcaaata atattattgc cgatgatatt gctctagata tgattggtgt gctaaaaaat
     2701 aaaacgatga cacatgcacc acaacaaaat acttcgccag atgtaaaccc tgtatcacaa
     2761 aaaatagttg acgttatatc atcgcgtggt gccgaatact attcacagca ctacaacgat
     2821 atattaaaag aaacaggttt cgatttcaaa aacgatatga ttttgctgga tgcaggtcaa
     2881 ctactcgaag acaaaaaaat gtgggatggc ggcatttctc tgtatgaatc gtgtacaaaa
     2941 ctttttccaa atattattat aggttggaac aggcttggat attgccatct tcaaaaaggc
     3001 gataaagaaa aagcacgaac atgctggaaa aaatccctca ccattcgcac cgaaaataat
     3061 cgtgccgggg aatttttgaa atcaattaat aattaataaa catgctgccc gccattgatg
     3121 ctgtaatttg caccggtaat aaaatttgtt ttttccgatg caaggaacga aacaagatgc
     3181 gccacttctt cggtgccgcc taagcgcccc attggtattt gcgctacaat ttgttcgcgt
     3241 actttttcgg ccactgccat taccatatcg gtaccaatat aaccgggaga aatagtattt
     3301 accgtaacgc catattttgc aacttccatt gccaatgttt ttgtaaaccc gtgcatgccc
     3361 gctttggcag cgctgtaatt ggcttgtcca aattgtccac gctgtccgtt taccgatgat
     3421 atatttatta tgcggccata cttgcgctcc atcattccat taataacatg acgagtgcaa
     3481 ttaaatacgc ttgtaaggtt ggtcgcaatt acagcatccc aatcttcttt actcattttc
     3541 gaaaaacgta tgtcgcgggt tatccctgca ttattcacaa gtgtatcaat ggggccaact
     3601 tcggcttcta tttttgcaat cattgcacct accgaatcgt agtcggtaac atcgccttcg
     3661 tacatatcaa ttacaaaacc ttcggcattt atttttgcct gccactcatc ggccttttgc
     3721 ttattgcgat agtttgcaat aacatgaaat ccatctgtgt acaattcttt acacatggct
     3781 gtgccaagcc caccagttgc accggttaca agcacaattc ttttcgattg cttatccata
     3841 aaataatttt aaaaaaatta agaaataaat tatcctttat aatcagtcag gaaattttat
     3901 aaactggcaa ataatgccac ccatcagcat aagagaaatt gcccaaaagc ccacattgat
     3961 aaaaatgtac ttaaaacttt ttctttcgaa caatgcattg acgcccataa caggcattac
     4021 aaaaaacaaa gagagtataa agccatgcaa catgccatgc ttaaatgtgc gaaaattttg
     4081 gccatactta gtcataaaat ctgtgtagta cgttcccatt gcagagttag gatcattaaa
     4141 acctggttca tccaccaatg ttgacattac gttatattga tgtatcacca ttgtttgtaa
     4201 aacgaatgcc aacataaaac tgaacaaaaa cgaaacgcca aaaataacac ccatgttacc
     4261 gcttttaatt ttttcttcgg tcatgcctga ggcattcatc caggctgtgc ccagcgtttt
     4321 tggattgtaa taaataaaac cgacaatcat tggtaccaaa gcggcaagcg caatgtaaac
     4381 taagttcatc atattttttt gttttagagg ttaagagcaa ggagacaatt attgagagca
     4441 aacataaata aatatgttat tcttcaaaca aataaattaa aaatctattt tgtaataaaa
     4501 caatggcaaa aatccaagtt gatattcttc gcgcaatggc gattgattag atgcattgtc
     4561 gaccggaaca taagtaaggc caagaacatt ttttgtattc aaaatattta caaggtcgag
     4621 cgcaatttcg tgtgtgaatt ttttcgtatt tacttttatt cctaatttaa aatcgaggcg
     4681 aaaataatct ttaaactttt ttacgttacg caacgaatct atcaccacca gttcgccagc
     4741 aaggttcgat gcatgaacat cggccggggt atagcgctga cctccggccc atgttacttt
     4801 ggctccggta gtaagtgtgg tgcggttttt tttgccaagt ttatactcgc gtgctgccag
     4861 tgcatttact ataaaattac cattgaaatc ggtattgcgc ttctcaccat cgcttccttt
     4921 gtaggtggaa ttatacagtg acgaagtaaa cagaaagaaa aaattgcggt taaaaaaacg
     4981 ttccagtgta aattctattc catagttttc gcctgccccg gcatttacaa gcgaatcggg
     5041 aaaaattctt gaaaacgatg agccctgatt taatatactg taagaagaat tttttatttc
     5101 tacaggaata ttcgaaagcg actgatagta cgtttcaatt tttatgcgtg aatatttgtt
     5161 taaagaataa tcgtatccca acacaatatg ttggctgtta aaaaacttca aatcattatt
     5221 gagcatgtta tattttccgg ctgagtcttt acgatgagca aaataaatat agtcgggcaa
     5281 catttgacta tgcataccat aacctgcgct caccgtactt ttttccgata cttgccagtt
     5341 gattcctact cgtggttcta ttgctttcga gttttcattt atggttaata actgtgcatg
     5401 tatacccccg ttaattgaaa gtacatcggt aattttatat ttgtattgta tgtatggttg
     5461 caccaaaaaa gcatcgccat tataatccag tctgttatcc cacacattta ttttttcgag
     5521 atagttgctg tcggccatat tgaaattaaa acgatcgaca ttgatgccgg ctttaataat
     5581 atgctgcaat ttaattcgtg tattaataaa ccatgccagc gatgttttct gtgtagcaaa
     5641 tttataattc aatttgggta ataaagtatc gacactaaaa tcgggattgc gtattaccaa
     5701 ttcgtgataa gcatgtgcac ctgcaagctg atggctcaat accgttttca tgtaagtgtt
     5761 tttacccaaa ggaaaagtgt gtgacactcc gccaacaaaa gtagaagaac caaaatattg
     5821 atcgcggtct ttatcgccat aaatttcttc ggtgggttcg gttttatcac ttaccaaaat
     5881 ttcgatatcg cttttaccac ccactccaaa tattgtaaaa ttacctgccc gctttgttgg
     5941 caaatttatt ttgattgttc catcctgata tttgggcact gcagaagttc caattggaat
     6001 gctcaatgca ctaaatacct gaaaagtgga atacctgtat gtagccagat aggagctgcc
     6061 atttgtgcgc gatatgggac cttcggctaa taattctgtt cccaaaaaac caagctgtgc
     6121 tgtaaactca tggtgctcgt tatttccatt gcgcaatcgc aaatcgaaca ctccggctat
     6181 ggcatttcca tattctgcag gaaatgctcc cgtcataaaa tcactattgg caaatgtttt
     6241 actatttaat aaattgatgg cacttcctgt tgttccggga acagcaaagt gatttggatt
     6301 cactatgtct acaccatcca gtttccacaa cacgccccag ggcgagttgc ctcgtataac
     6361 aatatcgttg cgcgaatcat cgcctccctg cacaccggca aaattcgatg ccatgcgtgc
     6421 agcatcatcg cggctgccgg catagcgttg cgtttcttcc acagtaaatg ttcgtgcact
     6481 tactgttgcc atttcgttta tcacatctgc tttgcttcct gccgaaaccg ttatttcttt
     6541 tatgtccata atacgcgctt gtagttcaac attcagcacc atttcttttg ccgatgaaac
     6601 tacaatgttg gacaacactg tttctttata acccagacac cggatggcaa tgcgatggcg
     6661 accaacagga acttttgcta ttttgtaatt accatcgtaa tccgttacgc cacccatagt
     6721 atttgatgta ctgtcatcgg caataactat agtggcacca aacaaaggtg tttttgtttc
     6781 tgcatctatc actttgcctt ttatattctg taccaaggtt gattgtgcaa ctatgcttgt
     6841 atacgtatgt aaaagcagaa gtgaaaaaag tattatcttt gttctattca aaattatcat
     6901 tgtcataggt tgattgaact ttatcgtata tggtttacga actatggtcg cttataataa
     6961 cccagcggcc atcaatatat tgccaaatca aagtgtagta tccgcttaaa gtatctttgg
     7021 tacgaagcaa ttcccatttg ccaaacacat aaacatactg ctcgcaaagt ggtgtcattt
     7081 tcaaatttgt aaattgcagt tttcccattg cttcagcatt ggggtacgac tttttataat
     7141 tggaaagtgt ttgctgccat ccatattgag gacccctttt tcctacaaac tgaaggctgt
     7201 ccgatttcca gtaacaaccc atgtaggctt ctaaattgcc gctattccac gatgcctgtt
     7261 gcgattgcat acatgcgctt atttctttta acgcattatt ttcggcttta aaaccggcat
     7321 atatcaaagt tgacagtgca acaaatgcga ctataaatat tttaaatgtt gaattcatat
     7381 tcgaaaaaat gatttgataa aacagaagtg ttctttgtta gcaatgaagt gacgtttaaa
     7441 aatagttgtg tattcaattt cgaagaaaca aaaatatttt tgtgcagtaa aactatcgga
     7501 aaaaagcata catcgtaaga aatgaaaaaa tattttctct ccagttattt atgtgacaaa
     7561 aaagcaattg aaatcgcctt gtataaaaaa tcgatggaga aaaaaaccga taacttagtt
     7621 gtttattttt gcttttgttt cttcttcaat ggcctgtaaa agatcgataa gttttttaaa
     7681 atcgaaaggc ttcgaaaaat aaaaatccat tccggaatca aaacattttt gtttgtcttg
     7741 ttccagcgca tttgctgtta tggcaattat ggtgcttctt ctgcgcatct ctatctcagt
     7801 atttcgtatt gcaagcgatg catcccatcc atccatttcg ggcatattga tgtccataaa
     7861 aattactgga taatatgttt tggtagccat agtaacagcc tctacaccat tgtaagcgat
     7921 gtcgcattgg tatccatgat tggctaataa ttttgatagt atcatgcaat tggtgggcac
     7981 atcatccact atcaaatatt ttggtgctgc aggttgtgtt gtgttttgaa taacaggtgc
     8041 cctgaataaa gacaagtcgt tgaggcgtga aactttattg gacacatatt taaaaaatgc
     8101 gcggtaacgc aaaggcaacg aatatttatc ggtatatgca gtataattgt ttgcctgatt
     8161 gcgggcatca aaatttaaca ccatcattcc ctgcaatact tttgtgttat gggcaaacca
     8221 ggtagaaata acttcgttgc tttcaatatg cttctgatgt attatgattg gttgattcac
     8281 aaatgaatcg atgtttgaaa caaaattttg ccatgaaata tactgtgagt tgatgccata
     8341 tttttttgct tcgttcatga tgttttgtat agcaacattt tgtgtttcca ttgcaaacaa
     8401 aggtgtgttg tttaattgct gcatcaatga tttctggtcg ataataaatt tattaaactc
     8461 cgaataatcg ttcgattcat gacatggcat attgagagta aatacggaac cctttccaga
     8521 ttcacttgct acagaaatat cgccattcat aagtgatgca aggcgtttgg agattgcaag
     8581 tcctaacccg gtgccacctc tacttactcc tttgtgggcc tttatctgtg tgaaagagtt
     8641 aaataatgtt cctatgtttt cggtcgatat tccaatgccg ctatcgcgca ccatgaattg
     8701 taaatctttg cccgattcat ttaccttcac atttaatgct acaaatcctg tgtcggtaaa
     8761 tttaattcca ttgctcaaca ggtttagcaa tatttgcttg aggcgtatgc tatcgctata
     8821 aattgctgtc ggcacttttt cgtcaatgta gtaggtaaaa tcaatttgct tttcaactgc
     8881 cttcgatgaa attatttcaa acacttcttc tattagctga ggcagataaa cagactcttc
     8941 ggcaagttcc attttgcccg attctatttt cgaaaagtcg agaatgtcat ttatcaatgc
     9001 cagtaatgta cgtccacttt gttgcaaacc attaataaat tcctgttgct catcatcgag
     9061 gggagtgtgt tgcatcaagt cggcaaggcc aataattcca ttgaggggag ttcttatttc
     9121 atgactcatt gatgccagga aagcactttt tgttgcagtt gcagtttcag caatttcttt
     9181 cgattttatt aaaacctgtt gggtattttt catgtcggta atatcatgaa acatatttac
     9241 catataaact tcaccagcgg caggggcatc aataaataca cggttaatct gcaaaaatcg
     9301 ttgttcatta ttaatgaatg ccgagctttc gaatccttta tgtgcttctt tttcattaaa
     9361 aatttcggtg taccgatcca tggtaagagg cacatccatt ttaaaatcgg ccgataattt
     9421 ttctaccgtg ctttttgtta tttcctgttg tgtgagccct gtcaatttgc aaaaggcatc
     9481 gtttgcattt attattttcc caaatttatc ggtaatcaat attccatcat ttgaatgttc
     9541 taatatactg cgtaacctgt tttcggaaga ctgcaaattg tgtgttcttt catttaccat
     9601 ttcttgcaaa acactggcat tccgtttttg ctgcaaatat tttagcaaag gatatatgat
     9661 agaggtggca gcaataaata ccaggatgaa aaaccaccat cgtgtataaa acgctctgtt
     9721 aattgttatt tggcttgatg ttacatcatc gctccattgg ccgttgtcac ctttagcacg
     9781 aatttttaaa aagtagttgc ccgaaggtaa attagtataa cgaatgttgc gcaaatcggc
     9841 tttgggaagt gtgaaccaat ctttatcaaa cccaacgagt tttacctgta cctgattgcg
     9901 tttttcgttt gtaagcgata tggcattgaa gtgaaaagtt agactgttat tactgtaatt
     9961 tatagcaaag tcgtgctcaa gaccataagt ttcgccaata gaattttcta cattcaataa
    10021 atttataatc ggcttttgaa ggttgttcaa attgtattcc tggtcgtaac atgaaactcc
    10081 tgcatttgta cctatccata accggccttt attatcgaga caaattgccg agcggtttgt
    10141 ttcgcggccc gacaatccat tttcagtaga ataaggccag gcatgataac ggtttagttt
    10201 ccacactccg tcattggtgc ctatccacag attattttta ttatcttttt gaataaaata
    10261 aaccggcagg gtatttaaaa attcatggtg tttgaatcgt gttgctttat tattgtgata
    10321 aacaaacaat cccaacgtat tgcctatgat tatatttcca ctaccatcat ctacaaggca
    10381 atatgcagaa acgttatcgc ttttattctc aaacagcagt ggttgtgttt ttccattttg
    10441 tctgcaaaat accccttggg tagtagcaat gtaatactca ccggctttgc caaaaaataa
    10501 tttacgcaca tattgctgtg aagtaaaaat ctcttccaga aaaatcaatt tcccatttat
    10561 caatttatac attgatgagg aacccactgc aattgcagta ccctgagcat cggttgtgag
    10621 cgaagtaatc tcttcgggat aattgctaaa aggtattact acactgtacc cttcatttgg
    10681 catcaacttg caaataccca actgcccggc aataaaataa acctggcctt tgttatcttt
    10741 tattgcatcg ccaattctat tactactcaa tggctggtta cctaccgaag taaactcatg
    10801 tgtggtaatt ttaccattat taaaaatgta atacccatat tttgagccga aaaaaaactt
    10861 ttcgttttcg agttgaatga tgctcgatac ttcgttgtcg ttattgttta ccggaggaaa
    10921 ataatttaca aaattgaatg aatttatttt gcctacacca cgttgcgaag tgaaccataa
    10981 attattttca cgatcaaaaa tcatatcggt aaaccatgtg atagtttcct gatcacgtag
    11041 cgaaaaattg aaaatctttt ttgtcgattt ttcgaggtaa taacaactca agtgagtaga
    11101 aataaaaacc ctgttattac catcgcccaa aatgagatca tcgcccgagt cataattttg
    11161 aataaaattg agcgaattat ttatacatgt aaatctatca ttctcgatat agcctaccca
    11221 ggtgttgcca aacatccatg tagttgtatt gccttttgta gtggatggtt catcagtcat
    11281 tcccctcaca ggcagaaacg aaggaggtat aatagcattt atattaaatt gcagtttgcc
    11341 attgctccat tcatataatc gcgcttcggt gcaaaaaacc acactgcttt tgtattttgc
    11401 aatattgaga attcgctcac ctgcatatcc gttggtttta ttgtaagtgt accacttgtt
    11461 ttgataatta acacgaaaac tggtagctgt agaatacgca atcaccattt taccgttaat
    11521 agttgctgca aaacatttgg aaatagccgc aaaggcgcaa gtatcgggtt ggcttatttt
    11581 tatggatgaa taaatcttgc catcatattt aatgatatcg atattatcat ttttcgggaa
    11641 aaaataaatg ttgccatttt tatcatgttc cagatgttcg tactcgcggg catctatggt
    11701 gtcggcaata tgttttacgg ttgtccattc ggtgccatca aaataggcaa gcccgttaga
    11761 agtgcttacc cacaaacgcc cattagcatc ctgtgccaat tcatttgaca cataggacgg
    11821 caagccgggc gattcgttgt acgatttgta cttatacgtt tgaccaaata atgttatgga
    11881 cgaacaaaaa gtcagcaaac aaaaagaaac aactaaagca cctattgatt ggggag
//
LOCUS       DAORQA010000040        11893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1459_length_11893_cov_10.408599, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000040 DAORQA010000000
VERSION     DAORQA010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11893
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1459_length_11893_cov_10.408599"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..435)
                     /locus_tag="PK736_04265"
     CDS             complement(73..435)
                     /locus_tag="PK736_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRC32638.1"
                     /translation="MLINELSKKTGITAHTIRYYEKYGLIKGVRKNEIKSNKYYHYDD
                     EVVEKLLLIRDAKSIGFTLSEIKQLLDAWFSKKFNVAKKVAVLDEKLISIDEKIKQLK
                     EVKKLIAQFKREVEENDC"
     gene            538..1320
                     /locus_tag="PK736_04270"
     CDS             538..1320
                     /locus_tag="PK736_04270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC32639.1"
                     /translation="MKQTALITGASNGIGLELARVHASKGGDLVLVARNKAKLDELKL
                     ELEKQFKISVYVIAKDLSAIDSAKEVYDETTKQNIQIDYLINNAGFGDFGMFYQTDWN
                     KELQMINLNITTLTLFTKLYLQDMIKRRSGRIMNVASTAAFQSGPNMAVYFATKAYVL
                     SFSEAVDNEVSDKGITVTTLCPGATESGFQAAAAMEESNLVKGKKLPTSREVAVYGYD
                     AMMKGKTVAIHGLMNYIMANSVRFTPRALVVKITRQIQDKAK"
     gene            1323..1817
                     /locus_tag="PK736_04275"
     CDS             1323..1817
                     /locus_tag="PK736_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32640.1"
                     /translation="MEHISKYFNAEKNESYLFILIGILAVGIGAYFLFKNTNTFYKVI
                     AYPIILIALIQITVGTTIVLRTSADIKNAEQYHIDKGKINEIEIPRMKKVMDSFSLYK
                     KIEIALIIIGIAMFVFFRNKELLRGIGLGLLLQSSIMLTLDLFAEARGKKYIEELKSY
                     SQLK"
     gene            2186..3439
                     /locus_tag="PK736_04280"
     CDS             2186..3439
                     /locus_tag="PK736_04280"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRC32641.1"
                     /translation="MNQNQKNELLEALFDHVAESIIVADTEGIIKLANPASEKLFGYN
                     ESEMVGKRIEMLMPQNFAQKHKNHREGYNKNPNPRSMGIGLDLFAQKKNGTLFPVEIS
                     LTPFNSSDHCCVIAFIIDITKRKKVEHEITEKQNELEALTIELKTSNERLESKVLSRT
                     KVLSEALSELEKSQQELKEALEKEKELNELKSRFVSMASHEFRTPLTAILSSASLIDE
                     YITTEQQDKRTRHVNRIKSAVNNLNDILSDFLSISKIEEGKIIANYSVFDVHELITDT
                     QGEMRGLLKDGQDVNYTHQGEKLIYSDKKLIRNVIINLVSNAIKFSPEDSQIEIATAV
                     NDAQVIIKIKDNGMGISKEDKTHLFERFFRGQNATNIQGTGLGLNIVANYVELLNGFI
                     ELESELDIGTEFIITLPNKKMNQSK"
     gene            3510..4568
                     /locus_tag="PK736_04285"
     CDS             3510..4568
                     /locus_tag="PK736_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC32642.1"
                     /translation="MYNILLIEDNKAVRENTAEILELAGYEVATATDGKKGVEQVGKQ
                     KPDLIICDIMMPVLDGYGVLHLLGKNPETASIPFIFLTAKAERSDLRKGMEMGADDYI
                     TKPFDKLELLNAVESRLKKLELLKKEYQKDMDGLQVFIEDMAGKEALIKFIDGRKVMK
                     YNKKQNVYMEGHNPIGIYYLNSGKIKIFKSHDHGKEFIVGLLKEGDFFGYTALLDQKP
                     YTETAEAIGDCEVIFLPKEDFFTLIYNNREVARKFLHILTQNVNEQQDKLVELAYSSV
                     RKRVAEALIELYDKYSGQEKNPMRISVSREDLANMVGAATESLIRTLSDFKEEKLIDI
                     KEGKINLVSEKRLRALKN"
     gene            complement(4580..4993)
                     /locus_tag="PK736_04290"
     CDS             complement(4580..4993)
                     /locus_tag="PK736_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32643.1"
                     /translation="MVYKINIAKTKTIGELQKEFNTIFPFLKIEFFIDPHVMGKGSAK
                     NKMINNATQIGDIQKIKDVHTIALESNFSVSNLEQKFENELGLYIQVFRKSGNVWLET
                     SATDSWTLAEQNEEGEMLAKAFRQERENFDDHDVY"
     gene            complement(5007..6383)
                     /locus_tag="PK736_04295"
     CDS             complement(5007..6383)
                     /locus_tag="PK736_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC32644.1"
                     /translation="MFIQQIYTGCLAQASYYIESNGEAVIIDPIREPDPYLNLAKARN
                     AKIKYVFETHFHADFVSGHIDIADRTGANIVFGPHAKPAYPAYIARHKEFFNVGNCTI
                     QLLHTPGHTIESSCFLLYDENKITRAIFTGDTLFVGDVGRPDLMSGNKTKTELASMLY
                     DSLNEVIIQIDDDVLVYPGHGAGSACGKNLGIERYSTIGEQKKLNYALQPMSREDFIN
                     AVTENLTTPPSYFFKDASINMIGYKSFDDVIQQNHVALSVEKFCDFISKGCLVLDTRN
                     AEVFANGFIKGSLHIGLKGDFAPWAGTLIDLDRELILITEPGYEMETITRLARIGYEK
                     VAGFLEGGFAAWENAVMPVDVIENIDADDFFFLNDSLEYSIIDVRRYSEAKSENIKRA
                     LIIPLENIMNQIKEFDKEKKYVLFCKGGYRSMIAASALKAHGINNLTNVSGGIEKIKK
                     YNPELISA"
     gene            6511..8916
                     /locus_tag="PK736_04300"
     CDS             6511..8916
                     /locus_tag="PK736_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HRC32645.1"
                     /translation="MSQILEKIACYHCGDECAGETVAMDDKFFCCNGCKMVYEILHEN
                     NLCSYYEMNNAPGKKQLNFTGKRYSFLDLPAVKQKLINFQNKNEVQVTFQIPDMHCSS
                     CIWLLENFKKINHGVISSRVNFLKKELFIAFDENKTSLRTIVEQLAQTGYEPSIHLDS
                     MAKKETKKNNRSRIIRIGVAGFCFANIMMLSFPEYFSSGIIIDSGLKQFFSYMILLLA
                     LPVFFYSASEFFIKSFQAIRHRTSSIDIPIALGIVAIFVRSAYEIITQTGAGFFDSGS
                     GLVFFMLIGRWFQDFTFDSMAFDRDYKSYFPISVTTIKNNIETDVVVNDLKISDRIII
                     RNNEIIPVDAILLKGNANIDYHFVTGESIPVNHEPGETVFAGGKQTGGIIELQVTKEI
                     SQSYLTQLWSRNFSENGVSKFEKIVQAISKWFIAVTLVIAFVAGAYWWQTDVHKAINA
                     FTAVLVIACPCALALSAPFTYGNILRILGKNKIYLRDYHTIEKLADVNTIVFDKTGTL
                     TKNKSAEVNYYGQQLTAEEEKMVFSLLRQSSHPLSKLLCKQFYVNGFFHVNNYIEETG
                     KGLSALVNNINVKAGSYAYVCNVTKLANDKGSNVFISINDVYKGYFVLSNSYRQGVAQ
                     MAANLSSNKYEMAILSGDNESEQNRLNILFADKAEMLFHQSPDNKLCYIKNLHNKNKK
                     VLMIGDGLNDAGALMQSDVGISLTEDTNSFTPASDGIAHASQLTNIASLLTYSKAAVR
                     IIIISFIISLLYNFVGLSFAVTGTLSPLIAAILMPISTISLVLFTVISSTIKGKWLGF
                     RVS"
     assembly_gap    9369..9468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9469..9661
                     /locus_tag="PK736_04305"
     CDS             <9469..9661
                     /locus_tag="PK736_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32646.1"
                     /translation="EFSPTKLVWTKPFPIPAFVGVFSNKASLDEAISDSKPARFLKPR
                     GFYAIDSARKNVNQKQIFF"
     gene            complement(9985..11874)
                     /gene="rpsA"
                     /locus_tag="PK736_04310"
     CDS             complement(9985..11874)
                     /gene="rpsA"
                     /locus_tag="PK736_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRC32647.1"
                     /translation="MVAEPEEEFDWESTGKKQQKYNTAEQQRLTDMYDKTLTAFVEHE
                     IVSGTIIGKTDRDVLVNIGFKSDGMIGIGEFKYMPEIKIGEKIDVYVESQEDKSGQLQ
                     LSHKKARALKSWDRVNLALNTDEVIQGYVKCRTKGGLIVDVFGIEAFLPGSQIDVKPI
                     RDYDQFVDKHMEFKVVKINNEFKNVVVSHKVLIEQEMENQKIEIMSKLEKGQVIEGTV
                     KNITSYGVFMDLGGVDGLLHITDISWGRINHPEEVLKLDDKINVVILDFDHEKKRIAL
                     GLKQLTPQPWDALDESIKAGDRIKGKVVVLADYGAFIEVLPGVEGLIHVSEMSWSQHL
                     RSPNEFMKVGDEVEAVILSLERPERKMSLGLKQLTPDPWGVITEKYPVGSKHTAKVRN
                     FTNFGVFAELEEGVDGLIHISDLSWSKKIKHPSEFTKVGDEIAVVVLEVDLENRRLSL
                     GHKQVEDNPWETYETVFTVGSVHEGTVGKVTDKGAQVLMPYGVEAYAPYKHLFKEGNV
                     LAKPDEKLNFRVIEFSKENRKIVVSHKVLYEEAAAGAKIAANAEEAKQMEQDAKTLKK
                     VKESVEKTTFGDLNSLAALKTDLEQGELEKAKVKLAQLADKKEDAAEETTQPEVDKKE
                     EAPDA"
BASE COUNT         3732 a         2242 c         2201 g         3618 t
ORIGIN      
        1 ttgttaacaa tgaaaaaaac aaatagttgc gttgaattga acttttacat caataaaaca
       61 taaaatcttt tatcaacaat cattctcttc tacttcgcgt ttaaattgtg caattagttt
      121 tttcacttct ttaagttgtt ttattttttc atctatagaa attagttttt catcgaggac
      181 ggcaactttt tttgccacgt taaacttctt actgaaccaa gcatcaagca actgttttat
      241 ttcactaagg gtaaaaccta ttgactttgc atctcttatc aatagcaatt tctctactac
      301 ttcgtcatcg tagtggtaat atttgttgct ttttatttca tttttgcgaa cacctttaat
      361 caatccgtac ttttcgtaat agcggattgt gtgggcggtg atacctgtct ttttcgatag
      421 ttcgtttatt aacatagtgc aaaattacct gtttaaaatt aatttacaac cttagaccct
      481 actctaatgt tgcaggtttt aaaataatgg atcacctttg ccaaaaaata aaacgatatg
      541 aaacaaacag cattaataac cggagccagc aatggtatag gattggaact ggcaagggta
      601 catgcatcca agggaggcga cctggtgcta gtagcacgaa acaaagcaaa gctcgatgaa
      661 ttgaaactgg aactcgaaaa gcagtttaaa atatctgttt acgttattgc caaagattta
      721 tcggcaatag attcggcaaa agaagtatat gatgagacaa caaagcaaaa tatccaaatc
      781 gactatctga taaataatgc cggctttggc gacttcggaa tgttttatca aacagactgg
      841 aacaaggaac tgcaaatgat aaatttaaat attacaacac ttactctttt tacaaaattg
      901 tatttacaag atatgataaa acgcagaagc ggtcgaataa tgaatgttgc ttccacagcg
      961 gcatttcaat cgggccctaa tatggctgta tactttgcta ccaaggctta tgttttaagt
     1021 ttttcggaag cagttgataa tgaggtaagc gacaaaggaa taacagttac cacactatgc
     1081 cccggtgcca ccgaaagtgg ttttcaggcg gcagctgcca tggaagaaag caacctggtg
     1141 aagggcaaaa aactacccac ttcgcgtgag gtggccgttt atggttatga cgctatgatg
     1201 aaaggcaaaa ccgttgccat acatggctta atgaattata ttatggctaa ttctgttcgg
     1261 tttacaccac gcgctttagt agtgaaaatt acccgccaaa tacaagacaa agctaagtaa
     1321 taatggagca catatcgaag tattttaatg cagagaaaaa cgaaagttat ttgtttattc
     1381 ttattggaat attagcagta ggaataggcg cttacttttt atttaaaaat acaaatacct
     1441 tttataaagt aattgcctat ccaattatat tgattgcgct tatacaaatt actgtaggca
     1501 ccaccattgt attacgcacc tcagccgaca ttaaaaatgc ggagcaatat catatcgaca
     1561 aagggaaaat aaatgaaatt gaaattcccc gaatgaaaaa agtgatggat agtttttcgc
     1621 tttataagaa aatagaaatt gctttgataa ttataggtat tgcaatgttt gtatttttta
     1681 gaaataaaga actcttgaga ggcataggtt tgggtttatt attacaaagc agcatcatgc
     1741 taacattaga tctatttgcc gaagcccgtg gtaaaaaata catagaagaa ttaaaaagct
     1801 attcacaact aaaataacag ttccgtttat ttgaattata gcatcgcgag ccccttttga
     1861 gcttcatggc aaattataca tttaaatctt atttgatttt ttcatgatat ttatcatgtt
     1921 ttttaatgat ttgcagcact ttatcacggt tcaaaactaa acacttttga gggcttagtg
     1981 tgactgcttt gcatgttaag aaaatttgtt ttgggtctgc ttatttagca agcacatagt
     2041 atccttcgca aaatgattga tagattgtgt atacatatta ttcatcacca aaataaaact
     2101 caaaccattg aagatttatt aaatacattt gacaaataaa cctgcaacaa acgcaaacaa
     2161 aaaaagcacg tgctacaata ttaaaatgaa tcaaaatcaa aaaaatgaat tgctggaagc
     2221 attgtttgat cacgttgccg aaagtattat tgttgcagac acggaaggca ttataaagct
     2281 tgcaaatccg gcttcggaaa aactctttgg ctataatgaa agtgaaatgg taggtaaacg
     2341 gattgagatg ctgatgccac agaattttgc gcaaaaacac aaaaaccatc gtgagggata
     2401 caacaaaaat ccaaatccaa gatcgatggg aatagggttg gatttatttg cgcaaaaaaa
     2461 aaatggaacg ctgtttcctg ttgaaataag tttaacacct tttaactcgt ccgaccattg
     2521 ttgcgtgatt gctttcataa ttgatattac caagcgcaag aaagttgagc atgaaattac
     2581 tgaaaagcag aacgaattgg aagcattaac catagaatta aaaacaagca acgagcggtt
     2641 ggaatcgaaa gtgcttagcc gaacaaaagt actcagcgaa gcattgagcg aactggaaaa
     2701 atcgcaacag gaacttaaag aagcgctgga aaaagaaaaa gaattgaacg aattgaaatc
     2761 gcgttttgta tcaatggcat cgcatgagtt tcgtactccc ctcacggcca ttctttcttc
     2821 ggcttcgctc atagatgaat atattaccac cgagcagcaa gacaagcgta cacgacacgt
     2881 aaacagaatt aaatcggcag ttaataacct taacgatata ctaagtgatt ttctcagcat
     2941 aagcaaaata gaagaaggaa aaataattgc aaattactct gtgttcgatg tgcatgaact
     3001 cattaccgat acacaaggcg aaatgcgtgg cttactcaaa gacgggcaag atgtaaatta
     3061 cacccatcaa ggtgaaaaat taatttactc cgacaaaaaa ctcattcgaa atgtaattat
     3121 taatctggtt tctaatgcta taaaattttc gcccgaagac tcacaaattg aaattgccac
     3181 tgcagtaaat gatgctcagg taataatcaa aataaaagac aatggaatgg gtatcagcaa
     3241 agaagacaaa acacatttat tcgaaagatt tttccgtggg caaaatgcta ccaatattca
     3301 aggtaccgga ctgggactaa acatagttgc aaattatgtt gaactgttga atggttttat
     3361 tgaactggaa agtgaactcg atataggaac agaatttata atcacactgc caaataaaaa
     3421 gatgaatcaa tctaaataaa ttaccagagg taatggatac atgaacgtga aaaggacaaa
     3481 agaaaattgc aaaaaataaa aaatacaaaa tgtataacat tttattaatt gaagataaca
     3541 aagcagtgcg cgaaaacact gccgaaatac tggaacttgc agggtatgaa gtggctaccg
     3601 ctaccgatgg caaaaaagga gtagaacaag taggtaaaca aaaacccgat ttgataattt
     3661 gcgatattat gatgcctgtt ctcgatggct atggtgtatt gcatttgctg ggaaaaaatc
     3721 ctgaaactgc atctattcca tttatttttt taacagctaa agcagaaaga agcgacttgc
     3781 gcaaaggaat ggagatgggt gccgatgact atatcactaa accatttgat aaactcgaat
     3841 tgttaaatgc tgtagaaagc aggttgaaaa aacttgaatt gctcaaaaaa gaatatcaaa
     3901 aagatatgga tgggctgcaa gtttttattg aagacatggc cggaaaggaa gcgttgatta
     3961 aatttattga tggtcgcaag gtgatgaaat acaacaaaaa acaaaacgta tacatggagg
     4021 gtcacaatcc aattggtata tactatttga acagcggaaa aataaaaata ttcaagagtc
     4081 atgatcatgg taaggagttt atagtaggtc tactaaagga gggtgatttt tttggataca
     4141 ccgccctgct cgatcaaaaa ccgtacaccg agacagccga agccattggc gattgcgaag
     4201 tgatatttct gccaaaagaa gatttcttta cgttgatata taacaaccgc gaagtggcga
     4261 gaaaatttct ccatatccta acacaaaacg ttaacgagca acaggataaa cttgtagagt
     4321 tagcctacag ttctgtacgc aagcgcgtgg ccgaagcact tatagagttg tacgataaat
     4381 atagtggcca ggagaaaaac ccaatgcgta tttctgtttc gcgcgaagac cttgcaaata
     4441 tggttggtgc tgctaccgaa tcgcttatac gtacgttgag cgattttaaa gaagaaaaac
     4501 taatagatat aaaagaaggg aaaataaatt tagtttctga aaagcgattg cgcgcactaa
     4561 aaaattaaat ctttggtgtt taataaacat catgatcatc gaaattttca cgctcttgcc
     4621 tgaaggcctt agccaacatt tcgccttctt cgttttgttc agcaagagtc cagctgtcag
     4681 ttgccgaagt ttctaaccac acatttcccg actttcgaaa aacctgaata tataacccta
     4741 attcgttctc aaatttttgc tccagattgc tcaccgaaaa attactttct aaggcgatgg
     4801 tatgtacatc ttttattttt tgtatgtcgc caatttgggt tgcattattg atcattttat
     4861 ttttcgcact gcctttgccc attacatgag gatcgataaa aaattcgatt ttcagaaatg
     4921 gaaaaatagt gttgaattcc ttttgcaatt cgcctatcgt ttttgtctta gcaatattta
     4981 ttttgtaaac cataatcaaa agtctgtcat gccgaaatta attcggggtt atatttttta
     5041 attttttcga taccgccact tacgtttgta agattattta ttccatgtgc tttaagtgca
     5101 gaagccgcaa tcatcgatcg ataaccccct ttacaaaaga gcacatattt tttttccttg
     5161 tcgaactcct taatctggtt cattatattc tccagaggaa taatcaatgc acgttttata
     5221 ttttcggatt ttgcttcgga atacctgcga acatcaatta tagaatattc caaactgtcg
     5281 ttgagaaaaa agaaatcatc ggcatcgata ttttctatca catccacagg catgacagca
     5341 ttttcccatg cagcaaaacc gccttcaaga aaaccggcaa ctttttcgta acctattctc
     5401 gcaagccgcg taatggtttc catttcgtaa cccggttccg ttataagaat taattctcta
     5461 tccaaatcta tcagtgtacc tgcccatgga gcaaaatcgc ctttcaatcc aatatgtaaa
     5521 gaacctttaa taaaaccatt tgcaaatact tcggcattac gtgtatcgag cacaaggcaa
     5581 cctttgctta taaagtcgca aaatttttcg accgataatg caacatgatt ttgttgaata
     5641 acatcatcaa aacttttgta accaatcata tttatactgg cgtctttaaa aaaatatgag
     5701 ggcggagtgg taagattttc ggtaactgca tttataaaat cttcacgtga cataggttgc
     5761 aaagcatagt tcaatttttt ttgttcgcct atagttgagt agcgttctat tcctaaattt
     5821 ttaccacatg ctgaaccggc accatgtccc ggataaacta aaacatcatc gtctatttga
     5881 ataataactt cgttcagcga atcatacaac atggatgcta attctgtttt ggttttattt
     5941 ccgctcatca aatcgggacg gcctacatca ccaacaaata aagtgtcacc ggtaaaaatt
     6001 gctctggtta ttttgttttc atcatacaat aaaaaacaag acgactctat ggtatgaccc
     6061 ggagtgtgca gcaactgaat agtacaattg ccaacattaa aaaattcttt atggcgtgct
     6121 atatatgcag ggtaagcagg cttagcatga ggcccaaaaa caatgttggc tccggttctg
     6181 tctgcaatat caatatgacc cgaaacaaag tcggcatgga aatgcgtttc gaaaacatat
     6241 tttatttttg cattccgtgc ttttgctaga ttcaaatatg gatccggctc gcgtatagga
     6301 tctataatta ctgcctcacc attcgactct atatagtaac ttgcctgtgc taaacatccc
     6361 gtatatattt gttgaataaa cataaattta ttttttacaa atattgagta ttcttgtcaa
     6421 ccaaaatatg acataagtca taaaacaata agacatatat caatttttcg ataaatagga
     6481 tgtattacaa ttgcaacata aagttataac atgtcgcaaa tacttgagaa aatagcttgc
     6541 taccactgcg gtgatgaatg cgcaggagaa acggtagcaa tggatgacaa atttttttgt
     6601 tgcaatggtt gcaaaatggt ttacgaaata ttgcacgaga ataatttgtg ttcctattat
     6661 gaaatgaata atgcccctgg taaaaagcaa ctcaacttca cgggcaagcg ttacagtttt
     6721 ctcgacctgc cggccgtgaa acagaaactg ataaattttc agaataaaaa tgaggtgcag
     6781 gttacatttc aaattcccga tatgcattgc agtagttgca tttggttgct cgaaaatttc
     6841 aaaaaaataa atcatggtgt aatttcttcg cgggtaaatt ttttgaaaaa agaattgttt
     6901 atagcatttg acgaaaataa aacatcgcta cgcactattg tagagcaact tgcacaaaca
     6961 ggttacgagc catctatcca cctcgactcg atggcaaaaa aagaaaccaa aaaaaataat
     7021 cgttcgcgaa ttatacgaat tggtgttgca ggattttgtt ttgctaatat tatgatgctt
     7081 agttttcctg aatatttttc atcgggaata atcattgatt cagggttgaa acaatttttt
     7141 agttacatga ttttgctact ggcattgccg gtgttttttt acagtgcatc ggaatttttt
     7201 atcaaatctt ttcaggcgat aaggcacaga acttccagta tcgatattcc aattgctttg
     7261 ggcattgttg ccatttttgt tcgtagcgct tatgaaatta tcactcaaac aggagcaggt
     7321 tttttcgaca gtggtagtgg tcttgttttc tttatgctta ttggccgctg gtttcaggat
     7381 ttcactttcg attctatggc attcgatcgc gattacaaat cgtattttcc tatttcggtt
     7441 acaaccataa aaaataatat tgaaactgat gtggtggtga acgatttaaa aataagtgac
     7501 agaataataa ttcgcaacaa cgaaattatt cctgtggatg caatactact aaaaggaaat
     7561 gcaaacatcg attatcactt tgtaacaggc gaatcgatac ctgtaaatca tgaaccgggt
     7621 gaaacagttt ttgccggagg aaaacaaacc ggaggaataa tagaattgca ggtaacaaaa
     7681 gaaatttcgc aaagctatct cacacaatta tggagcagaa atttttcgga aaatggtgtg
     7741 agtaaattcg agaaaattgt gcaggcaata agcaaatggt ttattgctgt tacgttggta
     7801 attgcttttg tggccggtgc atactggtgg caaaccgatg ttcacaaagc catcaatgct
     7861 tttactgctg tgctggttat tgcatgccct tgtgcacttg ctttatcggc accatttact
     7921 tacggaaata ttttacgcat actaggcaag aacaaaattt acctccgcga ttatcacacc
     7981 attgaaaagc ttgccgatgt taatactatc gtttttgata agaccggaac gctgacaaaa
     8041 aacaaatcgg cagaagtgaa ttattatggt cagcagctta ctgccgaaga agaaaaaatg
     8101 gtattctctt tattgcggca atcatcgcat ccccttagca agttattgtg caaacagttt
     8161 tatgtaaatg gtttttttca tgtgaacaat tatattgaag aaactggaaa aggattatcg
     8221 gcattggtaa ataatataaa cgtaaaagcg ggaagttatg cttatgtatg caatgtcact
     8281 aaacttgcca atgataaagg aagtaacgtt tttataagca taaacgatgt atataaagga
     8341 tattttgtat tgagcaattc gtaccggcaa ggtgtggccc aaatggcggc aaatttatca
     8401 agcaataaat atgaaatggc cattcttagc ggtgataatg aaagtgagca aaaccgcctt
     8461 aatattcttt ttgcagacaa agccgaaatg ctttttcatc aatcgcccga caacaaactt
     8521 tgctacataa aaaacctgca caataaaaat aaaaaagtgc taatgatagg tgatggactt
     8581 aatgatgccg gtgcactgat gcaaagcgat gtgggcattt cacttaccga agataccaac
     8641 tcatttacac ctgcaagcga tggtattgca catgcatcgc aacttacaaa tattgcaagt
     8701 ttattaacgt attctaaagc agcagtgcga ataataatta taagttttat tatttcgttg
     8761 ttgtacaatt ttgtagggct atcgtttgcc gtaaccggta cattatcacc attaattgcg
     8821 gctatcctta tgcccataag cacaataagt cttgtattgt ttacagtaat ttcaagtact
     8881 ataaaaggta aatggttggg gtttagggtt tcatagcctt tgttggttct cgctatcctt
     8941 ggttggcgtc cccgccaacc atcagtctat gcatcgtagc ggttggcggg gacgccaacc
     9001 gaggatagtg aatgcagttg gcgggacgcc aacggagcat agaaagctag caatgcaatg
     9061 ggttcaaaaa aaaacttcct cccgcctttg ctggagtttt ctccaacaaa gctagttagg
     9121 aagtcatttc ggatttcaaa cccgccaggt tttataaacc ttgcgggttt tatgcgatag
     9181 attcggctcg taagaatgtc aaccaaaaac agattttttt ttgattttaa aacctcgata
     9241 ttttaaacga tttgttgcgg ttggcgggga cgccaaccgg ggacagaact ctcgcccacc
     9301 ctacgccttt gttggagttt tctccaacaa agctagtttg gacgaagcca tttccgattc
     9361 ccgcctttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg agttttctcc
     9481 aacaaagcta gtttggacga agccatttcc gattcccgcc tttgttggag ttttctccaa
     9541 caaagctagt ttggacgaag ccatttccga ttctaaaccc gcaaggtttt tgaaacctcg
     9601 cgggttttat gcgatagatt cggctcgtaa gaatgtcaac caaaaacaga tttttttttg
     9661 attttaaaac ctcgatattt taaacgattt gttgcggttg gcggggacgc caaccgggga
     9721 cagaactctc gcccacccta cgcctttgtt ggagttttct ccaacaaagc tagtttggac
     9781 gaagccattt ccgattctaa acccgcgagg ttttgaaacc tcgcgggttt tgtgcgatta
     9841 gttgcagtag gcgataactc caaccgagga cagaagacag aaataaaagc aatgattgaa
     9901 gcggttttgg gtagtgtgca aaaaaaaact tcctcaccat tacgatgagg aagtttaatt
     9961 attttacaaa aagtaaaccc tgaattatgc gtccggagct tcttcctttt tatctacttc
    10021 aggctgcgtt gtttcttcag cagcatcttc ttttttatcg gcaagttgtg ccaatttcac
    10081 ttttgctttt tcaagttcgc cttgctcaag gtcggttttc aaagctgcta aactgttcaa
    10141 atcgccaaag gttgttttct caacagactc tttcactttt ttcaatgtct ttgcatcttg
    10201 ctccatttgt tttgcttctt cggcatttgc agcaatcttt gctcctgcag cagcctcttc
    10261 atacaacacc ttgtgcgata ctacaatctt gcggttttct ttcgaaaatt caattacacg
    10321 gaaattcaat ttctcatcgg gcttagccaa cacatttcct tccttaaata aatgcttgta
    10381 tggagcataa gcttcaacac cataaggcat aagaacttgc gcacccttat cggtaacctt
    10441 accaacagta ccttcgtgca ccgaacccac agtaaacact gtttcgtaag tttcccatgg
    10501 attatcttca acttgcttgt gtccaaggct cagtctgcgg ttttccaaat caacttcgag
    10561 tactactaca gctatttcat caccaacttt tgtaaactca ctaggatgct ttattttttt
    10621 gctccacgat aaatcgctga tgtgtattaa tccatcaaca ccttcttcca attcggcaaa
    10681 tacaccaaag tttgtaaagt tgcgcacctt agcggtatgc ttagagccta caggatattt
    10741 ttcggtgatt acaccccaag gatcgggtgt aagttgtttt aagcctaacg acattttacg
    10801 ttctggtcgc tctaacgata aaataactgc ttctacttcg tcacccactt tcataaattc
    10861 gtttggcgaa cgtaagtgct gactccagct catttcgctt acgtgtatca atccttctac
    10921 gcctggcaat acttctataa atgcaccata gtcggccaat accactactt tacctttaat
    10981 tctgtcgcca gctttaatgc tttcgtcaag ggcatcccaa ggctgaggag ttaattgttt
    11041 caagccaagt gctatacgct ttttctcatg atcgaaatcg agaataacca catttatttt
    11101 atcatccaat ttcagtactt cttcaggatg attgatgcgt ccccatgata tatcggtaat
    11161 gtgcaacaag ccatcaacac caccaaggtc cataaacact ccgtaagaag taatgttctt
    11221 aacggttcct tctatcactt gtcctttttc gagcttgctc atgatttcaa tcttctgatt
    11281 ttccatctcc tgctcaataa gtactttgtg cgatactacc acattcttaa attcgttgtt
    11341 gattttcacc accttgaatt ccatgtgctt gtccacaaat tggtcgtaat cgcgaatagg
    11401 ttttacatcg atttgtgagc ccggtaaaaa tgcttctatt ccaaatacat ctactatcaa
    11461 tccgcctttg gtgcggcatt ttacatatcc ttgtataacc tcatcggtat tgagggcgag
    11521 gtttacacga tcccaactct taagtgcacg ggcttttttg tgcgaaagtt gtagctgtcc
    11581 acttttgtct tcttgcgact ctacgtacac atcaatcttt tcgcctattt taatttcggg
    11641 catgtactta aactcgccta taccaatcat tccatcgctt ttgaagccga tgtttacaag
    11701 cacatcgcgg tcggttttac caatgatggt accacttaca atttcgtgct ctacaaatgc
    11761 ggtgagggtt ttgtcgtaca tgtctgttaa gcgttgctgc tcggcagtgt tgtacttttg
    11821 ttgttttttg cctgtggatt cccaatcgaa ttcttcttcg ggttcggcta ccacgttagc
    11881 ggtttggttt aac
//
LOCUS       DAORQA010000041        11835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1467_length_11835_cov_14.155942, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000041 DAORQA010000000
VERSION     DAORQA010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11835
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1467_length_11835_cov_14.155942"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>11835
                     /locus_tag="PK736_04315"
     CDS             <1..>11835
                     /locus_tag="PK736_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRC32648.1"
                     /translation="LNDYRSLSFGDWNDTSNWERFDGTNWVGASVAPNAGNANNIVIY
                     GTVHTSSGTYNVDQLIITPNGIGSFTESTVNVIDGVGDDITCFGQLKTNDAGLNLAAG
                     AVVKMKPQGTGECSPCALAGPGELTIEQNSTYLVSSGGSPFSLNNKVHINNYGNLNLQ
                     GASILNMLGDSNVVLNYGNFNIQMPSGFIATTGVGNLIKTFPNAVCNIETNFDIIKQP
                     DVIFENQGTLSLNQGAFNLQGSTTLAGKIIVNSGTTLFGTIDFDGDTLVNMGVLNCNS
                     FDFSGDTKQYLEGNGIITFMGLSNTFNLDVNGIITILDELKLYQGKIFTTTNGKLILD
                     SFCVVNNLDIQNNWVIGTIQKNIPLGTNINAVFHMGDSTRYAPNSAHFDNVIVAGNLV
                     ATQESADHFELATSGFNPTKTVNRNWDIQNNDVEFDSAEVFFEWDIADVDAGINVNQL
                     TIRKFSDSVWQDIGANATTDTSIVSQKFTTFSGFQVGQIVAPTNEYRTVTTSGIWSDP
                     ATWETFDGIAWVPATQAPYITDSTITIRTGHSVFVLSNLTIDELVVENGGVLSTNNVT
                     VTINDGPGVDVDFISGGFGLLGTWHVNTGASIDGGGFVNYYGPELINNGYIGLNFWDM
                     DDNANQTLSGTGKFKHLLLNTLSGTTLTSDMHVEQLFNINNGILHTGNFTLILDSGAT
                     AQNNGNGDDWIDGNMQQYYVAGNSSAWFLIGDNVDYYPISLTVDATVGGGVRASAIAG
                     DDANISSSNIDAAKSVNRHWKIDSAGVELSGLSVTLNWQPADIDAGADWNLFEVSKLH
                     DGIWTPLTVTNRTATSITASYTGNQLSTFQVGQPVCIINIPDANFKNALLANALINTN
                     SDLEIQCAEAAAFGGTINVSGLNISDLTGIESFSNLTGLDCSNNNLTAIDISDNSNLQ
                     TINCAVNAIVNLAFVGNANVQQLIIGNNLIPGSINLTGCTNMFNLNCANNLFTSLTLP
                     SSINLSMLQCQGNALTSLDVSNNTGLQFFDCNSNAITSLDLSTNTALTTILCADNQLS
                     SLNIQNGFNSNLVIFDATTNPLLTCIQVDDPSYMNANWSGGKDAGAIYSTNCACIVNI
                     PNFNFKAALLADTLINTNQDGEIHCSEAAAFTGTIQVNGLGITDLTGIEAFVNITELN
                     CNNNSLTTIDISNNTALTMLNLGYNLLTQLNTTNNTALLNLQCTNNQLTSLDVSTLTN
                     LNILVVYQNNLSSVNVTGLNFLGVLNVSINNLSSLDLSTNNSLAYFSCDNNVLTTLDL
                     SNQGQLISLNCYNNPLTSLDVSAASTNLQVIACFGTNITSLDLSNNSLLTNIDVYNNS
                     LLTSLNIQNGNNLNLVNFNATNQPLLSCIQVDDPSYMNANWSAGKDAGATYSIICPLP
                     CIVNIPDTAFKNALLANFLINTNSDSEIQCAEAAAYTGHMYINGLGIADLTGMEAFTA
                     LDGLTCYDNSLSTLDLSANTQLTYLFCANNLLTSLDLSNNLLLQTLLCGGNPITNLDL
                     SANTAITFFNSPGMSVTNLDLSAQTGLTWLNISTTPLLSSLNVQNGNNSNLTHFAAYG
                     NASLYCIQVDDSAFMNTNFGSVSNKDPQASYSSNCACTVNIPDTNFKATVLANPSINT
                     NSDGEIQCSEAAAYTGLLNLFATGVTDLTGIEAFTAITSLSINGNNIDTVNLSANTLL
                     TGIEAGGSQIASLTLPSTNILTYLVCEYNQLTSLDLSVCPALVEVWCRGNQLASLDIS
                     DNTALQYLYTQQNILAALNTSTNSALVTLTAQSNQLTSIDVSSNSALQVLNVSANLLT
                     SIDVSSNAALFTLTVAGNQITTLDLSANPSLINLQCQGNQLTSLNIQNGNNSNFLYFD
                     ATGNSSLTCIQVDSPAYMNTNWSGAINGGAFYSTNCSSLNITCAPAFTVCQDNINGAC
                     SVALLPPMPIQVGPSFTDRNVTYTGVSINGGGNAAVVAPGSSVSLSYSVVANFNINNV
                     YCPGCIYQGYIGIGGTFQTLQCEPSINVGYSNTYQGGNFVAPSTPGVYYLTYDYTLDY
                     TCQPRFFSNNPANAIGVLVVGPPFPVVVGGSGPVTVTNDAPSCFPLGTTTVTWTATDS
                     LNNTSTCTLDVTVTPATVYYRDRDNDGFGNASHVAYACSQPSGFILNNTDCNDDNSNI
                     TNSGCTASALNFDGVNDYAFVPGVVTNSFTIETWIKADASQSAFVWESAVSASDPSLE
                     GSTENLQFWVNDNTNVSTGPLVLGIWNHIACTFDAQTKLQSIFLNGVFVDSVTAIGVT
                     GISSGLFVGSRAGSLGFYPGSMDEMRIWTRALTAQEISDHYACEIQTPQNGLALNYHF
                     NQGIASANNSSDSILIDASGNGINGDLNNFALNGPTSNWVSPGGVVSGNACPLPCIVN
                     IPDTAFKNALLADTLINTNQDGEIQCAEAAAYTGTINIANLGISDLTGIEAFVNLTNL
                     DCSNNLLTSFDISNNIALTSLYCSGNQLATINISSNLNLLDLRCGANLLTTLNVSANT
                     ALTDLFCHNNQLTSLDISTNIALTALFCHDNLITNLSVSTNSALQYVDCYNNLLGSLN
                     FSSNAALYFLNCNNNQLTELNVQNGNNNNFTGFLALNNPPLTCIQVDDTAYSNTNWSA
                     GKDSGAVFSTNCTCLNFAITAIDGDSLIICAESNDSLGTKSFEILGSTAQYDYVFTDG
                     LSVTYTASGLSGNIVTITDIPAGTYTLSVSDYNGCGATTTLTAFITNCIIPYYEPPTN
                     DTIGNIIGSELTQLYNYPDSLADTTAANNIFLIDDSLGQVLIEVICNVNEYDTVLALI
                     QTSPYGMTDIIDNGDTTLIITGMFPIANLTKLDSLPDLINYVRPYYKPLLGAFAATGN
                     CVTQGDTAQYSNKAREGWSMSGSGVKIGVISDSYNTRFNNPAGNDVSNGDLPGTTNPN
                     YTTPVNVLEEYDYGRATDEGRAMLQIIHDVAPNAQLMFRSGFISAGNFADAITELADA
                     GCDIIVDDVTYITEPFFQDGLVAQKVNEVAQNGITYLTSAGNFGVKSYEGTFNPTPAP
                     AGYAGMAHNFGGGDIFQSVTLPKGTYTIAMQWDDNFYSLGQVPGALNDFDLYLVDQFG
                     NKLFGFNRDNIWGDPIEVLPFIVRNTVNANLIVTRKWGTSPIKFKYIIFRGEGVINEH
                     NLGSSTIIGQSNAAGAITVGAALYLNTPAFGVDPATAATFSSRGGMLIDGQSRNKPDI
                     IAPNGVNTTVSLGGKNIDGDLFPNFFGTSASAPHAAAVAALMIEAKRKYYNQEYTPAD
                     VKSDMQTTALDMGSTGYDDSTGYGLLKADAALQLLGNPIPQLNSYSLADTSLTPGTDS
                     VVVNLYGEYFANNAMVIFQYDTFPVTYLNNGHVNVIIPPFTGNPPITVCNPSLSTTGL
                     DGGVSDSLYFGSVPPQNVLVRCNDIVKKYGEKITPFSVSITINGVNIDSLGIDATDLG
                     LTDIVFNSNATSQSSVGFYFISASSNVTNLNDSASLALSELYNYHFANGTLLINRMPL
                     HVTPKDTALVFGASIKGKSFGFNYVYDDTNIEIADRANFLDSLETEHFSTLSNAILLI
                     DGKQAVNGFTLNDSDFVDLSFLVSGKAAVNGKQAVNGKQAVNSIVYDTTYYIPVSPAA
                     IFDYKIDSANAFLHNGKQAVNGKAAVNINGKAAVNGKAAVNGKAAVNGKAAVNGKAAV
                     NGKAAVNSSTFNDESNENAVLILDSLDVYGDPNDSIIGYTSMNMITGVTVGTWLIAPG
                     VLMSENFDITYGLGHLTITPETLTVKANDTAIYCFGDSAFFNATMGIFQYETGDTDVF
                     INTATYALLDDSNNVATNGSLSPGVYQIIPSGLIQKDSVANYIIDYKPGILTVDTPVS
                     ATVTTGTIACNGETTSLQINASNGFPPYAGDSTYTVSAGPYSFTVTDSKGCSVTTSGT
                     ITEPTPLTATPAPSAIPCNGGTSTVTITASGGTPPYTGDGVKTVSAGPYTFTVTDANG
                     CTTVVSGTVTEPTL"
BASE COUNT         3646 a         2238 c         2298 g         3653 t
ORIGIN      
        1 cgctcaatga ctatcgaagt ttaagctttg gagattggaa tgataccagc aactgggaaa
       61 gatttgatgg caccaattgg gtaggtgcct ctgtggcacc caatgccggt aatgctaaca
      121 acattgtgat ttatggaact gtgcatacaa gtagtggtac gtataatgtt gaccagctaa
      181 tcattacacc taatggaata ggttctttta ccgaatctac tgtaaatgtg attgatggcg
      241 tgggtgatga tattacttgt tttggccaat taaagacgaa tgatgccgga ttgaacttgg
      301 cagctggtgc ggttgtaaaa atgaaacctc aaggtacagg tgaatgtagc ccttgtgcat
      361 tggctggccc tggagaatta actattgaac aaaattctac atatttagtc agttcgggtg
      421 gcagcccatt ttctttaaat aataaagtac atatcaacaa ttacggtaac ctcaatttgc
      481 aaggggcctc tattttaaat atgcttggtg attcgaatgt ggtattaaat tatggcaact
      541 ttaatattca aatgccttca ggctttattg caactactgg agtaggtaat ttgataaaaa
      601 ctttcccaaa cgctgtttgc aacattgaaa ccaattttga tataataaaa caacccgatg
      661 ttatttttga aaatcaagga accttatcat taaatcaagg cgcatttaat ttgcaaggca
      721 gtactacgct tgctggaaaa ataattgtca attccggcac tacactcttt ggtactattg
      781 attttgatgg cgatactctt gtcaatatgg gtgtgttaaa ttgcaattct tttgattttt
      841 cgggagatac aaagcagtat ttggagggca atggcatcat cacatttatg ggtttatcta
      901 atacgtttaa tttggatgtg aatggaataa ttacaatact tgatgagtta aaattatatc
      961 agggtaaaat ttttacaact accaatggca aactcatatt agactccttt tgtgtggtga
     1021 ataatttaga catacaaaac aattgggtaa taggcacaat acaaaaaaat attccattag
     1081 ggaccaacat aaatgctgtc tttcacatgg gtgatagcac aaggtatgcg cccaattcag
     1141 ctcattttga taatgtaata gttgcaggca acttagtagc aactcaagaa tcggccgacc
     1201 attttgaatt agctacaagt ggcttcaacc ctactaaaac tgtcaacaga aattgggaca
     1261 tacaaaacaa tgatgtagaa tttgatagcg cagaagtgtt ttttgaatgg gatatcgcag
     1321 atgtagatgc cggtataaat gttaatcaac ttactattcg aaaatttagc gactctgtat
     1381 ggcaagatat tggagccaat gcaactacag atacaagtat tgtttctcaa aaatttacaa
     1441 cattcagcgg ctttcaggtg gggcaaatag tagcgccaac aaatgaatat cggacagtta
     1501 ctacctctgg tatatggtct gatcctgcaa cttgggaaac ctttgatgga attgcttggg
     1561 tgcccgcaac gcaagcccca tatattacag acagtaccat taccattcgt acaggtcatt
     1621 cggtatttgt attgagcaac cttacaattg atgaactcgt tgtagaaaat ggtggagtac
     1681 tcagcacaaa taatgtaacg gttactataa acgatggccc cggagtggat gtggatttta
     1741 ttagtggagg atttggttta ttaggaacat ggcatgtaaa caccggtgca tcaattgatg
     1801 gcggtggttt tgttaattat tatggccccg aattaataaa caatggttat ataggattga
     1861 atttttggga catggatgat aacgccaatc aaacattgtc aggcacagga aaatttaaac
     1921 acttattatt aaatacgctt tcgggcacta cacttacaag cgatatgcat gtggagcaac
     1981 tgttcaatat caataatggc attttacata cgggcaactt cacattgata ttagactcgg
     2041 gtgcaacggc tcaaaataat ggtaatggcg atgactggat tgatggtaac atgcaacagt
     2101 attatgttgc cggaaattct tcggcttggt ttcttattgg tgataatgtt gattactatc
     2161 caatatcatt aactgtggat gccactgttg gcggtggtgt aagagccagc gcaatagccg
     2221 gtgatgatgc caatattagt agttcgaata ttgatgctgc caaaagtgtc aaccgtcatt
     2281 ggaaaataga ttcggcaggt gtagaattaa gcggattatc cgtaacatta aattggcaac
     2341 ctgccgatat tgatgccggt gccgactgga atttatttga agtatcaaaa ctgcacgatg
     2401 gtatatggac accgcttaca gttactaacc gcacagcaac ctcaattaca gcaagttata
     2461 caggtaatca attaagtact tttcaagtag gtcaacctgt atgcattata aatattcccg
     2521 atgctaactt taagaatgca ttgttagcaa atgcattaat aaatacaaat agtgatttag
     2581 aaattcaatg tgctgaggcc gctgcatttg gcggaacaat taacgtaagt ggattaaata
     2641 ttagtgacct cacaggtatc gaatcgttta gcaatcttac cggtttagat tgttctaata
     2701 ataatcttac agcaatagat atttctgaca atagcaattt gcaaactatt aattgtgcgg
     2761 taaacgcaat tgtcaatctt gcttttgttg gtaatgcaaa tgttcaacaa ttaattattg
     2821 ggaataattt aattccggga agcattaatc ttactggttg taccaatatg tttaacctta
     2881 attgtgctaa taatttattc actagtttaa ctttgccttc atctattaat ttatcaatgc
     2941 tgcaatgtca agggaatgca cttacatcat tggatgttag caataataca gggttgcaat
     3001 tttttgattg taatagcaac gccattacaa gtttagattt aagcactaac accgcattga
     3061 caactatcct ctgtgccgac aatcaactca gttctcttaa tatacaaaat ggcttcaaca
     3121 gcaacttggt aatattcgat gctaccacca atccattgct cacatgcata caagtagatg
     3181 atcctagtta catgaacgct aattggagtg gtggcaaaga tgctggtgct atctatagta
     3241 caaactgcgc atgtattgta aatattccta attttaattt taaagctgcc ttgcttgcag
     3301 atactttaat caatacaaat caagatggcg aaatacattg ttcagaagct gcggcattta
     3361 ccggtacaat acaagtaaat ggtttgggca ttacagattt aacaggtatt gaggcattcg
     3421 ttaacatcac tgagttgaat tgcaacaaca attcattaac caccattgac atctcaaata
     3481 acacagcatt aacaatgcta aatttgggct ataacctatt aacacaattg aacactacta
     3541 acaatactgc ccttctcaat ttgcaatgta ccaataatca acttaccagc ttagatgtat
     3601 caacactgac taacttaaac atcttagtcg tctatcaaaa taatcttagc tcagttaacg
     3661 ttaccggact aaattttctt ggcgtactta atgtttctat aaataattta tcgtcactcg
     3721 acttatccac aaataatagc ctcgcttatt tttcttgcga caacaatgtg ttaaccacgc
     3781 ttgacttaag caaccaaggc cagttaattt cattgaattg ttataacaat ccgctcacat
     3841 cattagatgt ttcggcagca agtacaaatc ttcaagttat cgcatgtttt ggcacaaata
     3901 ttaccagcct cgatttatca aacaatagct tgctgaccaa tattgatgtt tacaacaaca
     3961 gccttttaac atcattgaat attcaaaatg gaaataacct aaacctcgta aatttcaatg
     4021 ctactaatca accgttgtta agttgtatac aagtagatga tcctagttac atgaacgcta
     4081 attggagtgc cggtaaagac gcaggtgcaa cctatagtat catatgtcca ttaccttgta
     4141 ttgtaaacat tcctgataca gcgtttaaaa atgcattatt ggcaaatttt ttaatcaaca
     4201 caaacagcga tagtgagata caatgtgctg aagcagctgc atataccggc catatgtata
     4261 ttaatggatt gggcattgca gatttaaccg gtatggaagc attcactgcg ttggatggac
     4321 tcacttgtta tgataactca ctttcaacgc ttgaccttag cgccaataca caacttactt
     4381 acttgttttg tgcaaataat ctattaacaa gtctggatct aagcaacaac ctattgctac
     4441 agaccttgct ttgtggaggt aacccgatta ctaaccttga cctttcagca aatactgcaa
     4501 taacattttt taacagcccc ggtatgtcgg ttaccaatct tgacctgtct gcacaaacag
     4561 ggttaacatg gttgaatatt agcacaacac cattattatc gagtttgaat gttcaaaacg
     4621 gcaataacag caaccttact cattttgctg cctatggaaa tgcatcgttg tattgtatcc
     4681 aggttgacga ctcggcattc atgaatacga attttggtag tgtcagtaac aaagacccgc
     4741 aagcatccta cagcagcaat tgcgcatgca cagtaaatat tcctgataca aatttcaaag
     4801 ctacagtatt ggcaaaccct tcaatcaata ccaacagcga tggagaaatt caatgttcag
     4861 aagcagctgc atatactggc ttattaaact tatttgcaac aggagttact gatttaaccg
     4921 gtatagaagc attcacagca atcacttctt tatctatcaa tggcaacaat attgatacgg
     4981 ttaatctttc agccaataca ttattaaccg ggattgaagc aggcgggagc cagatagcca
     5041 gtcttacact accatctact aatattctaa catatttggt ttgcgagtac aatcaactta
     5101 ccagtcttga tttatcagta tgtcctgcct tagtggaggt gtggtgccga gggaatcaat
     5161 tagccagctt ggatatatct gacaacactg ccttgcagta tttatatacc caacaaaata
     5221 ttcttgcagc cttaaatact tctaccaatt cagctttggt cactttaaca gctcaatcaa
     5281 atcaattaac aagtatagac gtatcaagca atagtgcctt gcaggtactt aatgttagtg
     5341 ctaatttatt aacctcaata gatgtttcat ccaatgctgc gctttttact ttaactgttg
     5401 ctggtaacca aatcacaact ttagatttat cagctaatcc atcattgata aatttacaat
     5461 gccaaggtaa tcaattaacc agtttaaata tccaaaatgg taataatagc aacttcttgt
     5521 attttgatgc tactggtaat agctcgttaa cctgtattca agtagatagt cctgcataca
     5581 tgaataccaa ctggagtggt gcaataaatg gaggggcatt ttacagcaca aactgttcgt
     5641 ctctcaacat tacatgtgct cccgccttta ccgtatgtca ggataatatc aatggagctt
     5701 gcagtgttgc cctactgccg cctatgccta ttcaagttgg cccttctttc accgatagaa
     5761 atgtaacata tacaggagtg agtattaatg gaggaggtaa tgccgcagtg gtagcaccgg
     5821 gctcatcggt ttcattaagc tattcagttg tagctaattt taatatcaat aatgtgtatt
     5881 gccctggctg tatttatcaa gggtatattg gaattggtgg cacctttcaa acccttcaat
     5941 gcgagccatc aattaatgtt ggatattcca atacatatca gggaggaaat tttgtggcgc
     6001 catcaactcc aggtgtgtac tatttgactt acgattacac attagattat acctgtcaac
     6061 ccaggttctt tagtaacaac ccggccaatg ctatcggtgt attggtggtg gggcctccct
     6121 tccctgttgt tgttggtggt tccggaccag taacggttac caatgatgct ccatcatgtt
     6181 tcccacttgg aacaacaact gtaacatgga ctgccaccga ttccttaaac aatacatcta
     6241 cctgtacact agatgtaacg gtaacccctg caactgtata ttatcgtgac agagataacg
     6301 atggatttgg aaatgctagt catgtggctt acgcatgctc acaaccttca ggatttattt
     6361 tgaataatac cgattgcaat gatgacaatt caaatattac caattcggga tgtacggcat
     6421 cggcattaaa ttttgatgga gtgaacgatt atgcatttgt acccggtgtg gttacaaact
     6481 cattcacaat agaaacatgg attaaggcag atgcaagcca aagtgcattt gtgtgggaaa
     6541 gtgctgtttc tgcatcggac ccaagccttg aaggctcaac cgaaaatctt caattttggg
     6601 taaatgataa caccaatgtt tctacaggac cattagtgct tggaatctgg aatcatatag
     6661 cttgcacatt tgatgcacaa actaaattgc aatctatatt tttaaacggt gtatttgtgg
     6721 attcagttac tgcaattggt gtaactggta ttagtagtgg tttatttgtt ggttcacgag
     6781 caggaagtct tggattctac cccgggtcaa tggatgaaat gcggatttgg actcgggctt
     6841 taactgcaca ggaaatttct gatcattatg cttgtgaaat tcaaacacca caaaatggtt
     6901 tggccctcaa ctatcacttc aatcagggaa ttgcttcggc caataattct tcagatagta
     6961 ttttaataga tgccagtggc aatggaatta atggagattt aaataacttt gctttgaacg
     7021 gaccaacatc gaactgggtt tctccaggtg gtgtagttag tggtaatgca tgtccacttc
     7081 catgtattgt aaatattcct gacacagcat tcaaaaatgc tttgttggct gatacattga
     7141 tcaatacaaa tcaagatggt gaaatacagt gtgcagaagc agcagcttat actggaacta
     7201 ttaatattgc taatttgggt atttcagatt taacaggtat tgaggctttt gtaaatctta
     7261 caaatttaga ttgttcaaat aatttattaa ccagttttga tataagcaac aacattgctt
     7321 taacgagttt gtattgcagc ggcaaccaat tggcaaccat taatataagt agcaatttaa
     7381 acttgcttga cttaagatgt ggagcaaatt tacttaccac tttaaatgta tcggcaaaca
     7441 cagcattaac tgatttgttt tgtcataaca accaattgac ttcattggat atttccacaa
     7501 atattgcctt aacagctctt ttttgtcatg ataatcttat tactaatttg tctgtttcaa
     7561 ctaattcggc attgcaatat gttgactgtt acaacaattt gttagggagt ctgaattttt
     7621 cttcaaatgc tgctctttac tttttaaatt gcaataacaa tcaattaact gaactgaacg
     7681 tacagaatgg caataacaat aattttacag gatttcttgc cctcaataat ccacctttaa
     7741 catgtattca ggtagatgat acagcatatt ctaataccaa ctggagtgct ggtaaagatt
     7801 cgggtgctgt ctttagcaca aactgcacat gccttaattt tgcaatcaca gctatagatg
     7861 gtgacagtct tataatttgt gctgagtcaa acgattcttt gggaaccaaa tcttttgaaa
     7921 tacttggatc aacagcccaa tatgattatg tgtttactga tgggttatct gttacttata
     7981 ccgcttcggg cttatcagga aatattgtca ctattacaga tattcctgct ggtacgtata
     8041 cactctctgt tagtgactat aatggatgtg gagcaacaac tactttaaca gcatttatta
     8101 ccaattgcat tattccatat tatgaaccac caacaaacga taccatagga aatatcatag
     8161 gctctgagtt aacgcaactt tacaattatc ctgattctct tgccgatacc acagcagcaa
     8221 acaatatttt ccttatcgat gattcgctgg gccaagtatt gatagaggta atttgtaatg
     8281 ttaatgaata tgatactgta ttggctttaa tacaaacaag cccttatggt atgacagaca
     8341 ttatagacaa cggtgataca acattgatta tcaccggaat gttcccaatt gccaatctta
     8401 ccaaattaga ttcgttgccc gatctgataa attatgtaag gccatattat aaaccactat
     8461 taggagcttt tgctgctaca ggcaactgtg tgacacaggg cgatacagca caatacagca
     8521 acaaagcccg cgaagggtgg agcatgagtg gtagcggtgt aaaaattggt gttatatccg
     8581 atagctataa tacccgtttc aacaaccctg caggtaacga tgtatccaat ggtgatttac
     8641 cgggaacaac aaatcccaat tataccacac cggtaaacgt attggaggag tatgattatg
     8701 gccgtgcaac agacgaaggc cgcgccatgt tacaaatcat tcacgatgtg gcgccaaatg
     8761 cacagttaat gtttcgctct ggttttataa gtgcaggtaa ttttgccgat gctattaccg
     8821 aacttgccga tgccggttgc gatattattg tggatgatgt aacatacatt acggagccat
     8881 tttttcagga tggattggta gcacaaaaag taaatgaagt ggcacaaaat ggaataacgt
     8941 atttaacatc ggcaggcaac tttggtgtaa aatcatacga aggcactttt aacccaacgc
     9001 ccgcaccagc cggatatgca ggtatggccc acaattttgg cgggggtgat attttccaaa
     9061 gcgttacctt gccgaaggga acatatacaa tagccatgca atgggacgac aatttttatt
     9121 cattagggca ggtgccgggt gcattaaatg attttgactt atatcttgtt gaccaatttg
     9181 gcaataaatt atttggtttc aaccgcgata atatttgggg cgaccctatc gaagtgttac
     9241 catttatagt tcgtaatacg gtaaatgcaa atctgattgt cactcgcaag tggggaacgt
     9301 ctcctattaa gtttaagtat ataattttcc gtggagaggg tgtaattaac gagcacaatt
     9361 taggctcatc cacaatcatt ggccaatcga atgctgcagg tgctattacg gtgggtgcag
     9421 ccttgtacct caatactcct gcatttggag tggaccctgc cacagcagcc actttctcat
     9481 cacgaggtgg aatgttgatt gatggccaat cgcggaacaa gcctgatatt attgctccca
     9541 acggtgtaaa tactactgtt tcgttaggcg gaaaaaatat agatggcgat ttgtttccaa
     9601 acttttttgg cacatcggcc tcagcaccgc atgcggctgc cgtggcagca ttgatgatag
     9661 aagcaaagcg gaaatattac aatcaggaat atactcctgc cgatgtaaaa tccgatatgc
     9721 aaacaacagc gttggatatg ggaagtaccg gttacgatga ttctaccgga tatggattgc
     9781 taaaagccga tgctgcttta caattattag gtaatccgat accacaacta aattcttatt
     9841 cccttgccga tacctctctg acacccggca ccgattcggt tgtggttaat ttatatggag
     9901 aatattttgc taataatgcc atggtaattt ttcaatacga tactttccct gttacctatt
     9961 taaataatgg acatgtaaat gttatcattc ctccattcac cggtaatcct ccaatcactg
    10021 tttgcaaccc ttctttatca acaacaggtt tggatggcgg tgtttctgat agcttgtatt
    10081 ttggttctgt tcctccgcaa aatgtgttgg taaggtgcaa tgacattgtg aaaaagtatg
    10141 gtgaaaaaat tactcctttt tcagtaagca ttaccatcaa tggagtgaat atcgattcgc
    10201 tagggattga tgcaaccgat ttgggcttaa ccgatattgt tttcaactcg aacgcaacat
    10261 cacaaagcag tgtaggattt tattttattt cggcctcgtc caatgttact aatttgaatg
    10321 actctgcatc gcttgcttta tcggagttgt acaattatca ttttgcaaat ggaactttgt
    10381 tgattaatag aatgcctttg catgttacgc ccaaagatac tgcgctcgtt ttcggggctt
    10441 ctatcaaagg caaatcattt gggtttaatt atgtttatga cgatactaat attgaaatag
    10501 cggatagagc taatttttta gattcgttag aaacagaaca tttttcaacc ttatctaatg
    10561 ctatactttt aatagatgga aagcaagcag ttaatggctt tacacttaat gattctgact
    10621 ttgtagattt aagttttctg gtaagtggta aagctgccgt aaatggaaag caagctgtaa
    10681 atggaaagca agctgtaaac tcaattgtat atgatactac gtattatatt cctgtttcgc
    10741 ctgcagctat tttcgattat aaaatagatt cggcaaatgc ctttttacat aatggtaagc
    10801 aagcagtaaa tggtaaagcg gcagtaaaca ttaatggcaa agcagcagtt aatggcaaag
    10861 cagcagtaaa tggtaaagca gcagtaaacg gtaaagcggc agttaatggt aaagcagcag
    10921 ttaatggcaa ggcagcagta aacagcagca catttaatga tgaaagtaat gaaaatgctg
    10981 tactgatttt agactcgctc gatgtttacg gagatcctaa cgattcaatt attggatata
    11041 catctatgaa tatgattacc ggtgtaactg taggaacatg gctgattgcc ccgggtgtgt
    11101 taatgtccga aaattttgat attacttatg gtcttggaca cttgactatt acacccgaaa
    11161 cacttacagt aaaagcgaat gatactgcta tttattgttt tggtgatagt gcgttcttta
    11221 atgccactat gggtattttt caatatgaaa caggcgatac agatgtgttt atcaatacgg
    11281 caacttatgc attgcttgac gattcaaata atgtggcaac taatggttcg ctttctccgg
    11341 gtgtatatca aataattcct tcaggtttga ttcaaaaaga ttctgtagca aattacatta
    11401 tcgattacaa acctggaatt ctaacagttg atactccggt ttcggcaact gttactactg
    11461 gcactatagc atgtaatggt gaaactacat cattgcagat caatgcatcg aatggatttc
    11521 caccttatgc aggcgatagc acatacactg tatcagcagg gccatactcg tttacagtta
    11581 ccgattccaa aggctgttcg gtaaccacca gcggcactat tacagagcca acaccactta
    11641 cagcaacacc tgctccatct gccatacctt gtaacggagg cacatcaact gtaaccatta
    11701 cagccagcgg aggtacccca ccctacactg gtgatggagt taaaactgtt tcggctggtc
    11761 cgtatacatt taccgttacc gatgccaatg gttgcacaac tgttgtgagt ggcacagtta
    11821 cagaaccaac tctat
//
LOCUS       DAORQA010000042        11537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1518_length_11537_cov_10.405766, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000042 DAORQA010000000
VERSION     DAORQA010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11537
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1518_length_11537_cov_10.405766"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4602
                     /gene="sprA"
                     /locus_tag="PK736_04320"
     CDS             <1..4602
                     /gene="sprA"
                     /locus_tag="PK736_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRC32649.1"
                     /translation="NGNNLKPAAVTDVELSKHDQREVLEQELFDRNNGTQTIPPLQVL
                     NLAYYPTERGPYNLDVGPTQFSKGVAADGSLNDPASRWGGIMRRLETNDFEAANVEYI
                     TFWLMDPFNEDNPNQADEGKLYFNLGQISEDILHDGFKLYENGLKCPNNTLPIDTCAW
                     GIYPFNPTNPTNAFDNDPDCRALQDVGYDGLPTSQEKSFFTGYLSALGALHGTASQAY
                     IKAEADPSSDNFQYFRGSNEPLLSNPSITSRYKNFNSPDGNSPTPQQSPEAYSTQATN
                     FPESEDINRDGNMDTYENYFQYEIKVSKADLKVGSNYITDKREVQVTTQDKRKRTIAW
                     YQYRIPVNDELRQKIGDIQDLRSIGFIRMFMKGFSAPIVMRFGRLELTRGEWRKYTNK
                     LISPGIYIDPPIAGNTSFEISAVNIEENKGKVPCNYVIPPGIIQEVNVGSAQLQRLNE
                     QSMVLRVCGLQDGDARAAFKTLELDLRNYKHVKMFVHCEDNLACTDDFDCGPGPVQDN
                     DLVLFVRLGTDNSQNYYEYEMPLKVTNYSGTAIKDADNIWKSANELDIDLEKLLNAKL
                     SRGNAGGALQAYVPYGQDGDRNIYVVGSPNLSNVRTIMIGIRNPKQGGIVNFGKDDGQ
                     AKCAEVWVNELRLTDFDERGGYAANARIATKLANLGNLTLSGSRKTIGFGGIEQKVNE
                     RQREDFVQYDIATTLELGKFFPDKISLSIPFYYSFGQIIAKPQFNPLDPDVPFQTALK
                     NLEADKAAELRRNTIDMTKSKSISFTNVRKNKTGGKSHIYDIENFNVSYSYTELYHRN
                     INLQYDLLRNHRASLGYNYNATVKPWEPFKKSKFVGNSKWIKLVKDVNVNPVPNSLSF
                     RTDLDRQYGEQLLRNNTTYKAIIDTTFNKYFNWIRAYDLRWDITKSIKFDFSANNQAR
                     VDEPAGRIDTQEEKDSIKYNVRQLGRNTQYGHNGNVNYQIPLNKIPLLDWISVNTKYG
                     FDYGWQAAPLRFDSVLNKTAPNAALGNTIQNSNTKSVNATLTFTQLYNKVPFFKKLLA
                     PKPPKPVTPKVKPEPKKDPNDTTKVKTPPPPKPPRGIPDVVRFIGKAIFCVKSAGGTF
                     SETNGTMFPGYRRRTEMLGQEYYTNEKGESVTAPGWGFVFGSQKDIRFDAAQNLWLSS
                     DTSFNSQYTRNFTQNITARSTIEPITGFRIELNATRNYARNLSENYRATPSGVYRSVG
                     ALETGNFSISYITWNTSFIKPNKDNTSDVFKTFDANRQIISRRIGVLNPYSSWAVDDS
                     GYVDGYSGTQQDVLTLSFLAAYSGRNPETIKNEQFLKIPLPNWRITYDGLSKMAWSKK
                     LFTSVNITHGYRSTYNINTFNTNLNYADPQNYGTSGVRDASGNFIPKYEATQVSITES
                     FSPLIGVDMTWKKNGLFTRMELKRDRNLSLTYTGIQLTEIRSNELTVGAGYRIPKFKL
                     PFKIRGKQVKLQNDLNITADFGMRKNITVIRKMQEKTDIPTSGMNSYTIKVAADYVIN
                     DRFNVRFYFDKVINEPVIATSFPNSNTQVGFAIRFTLAQ"
     gene            4636..5016
                     /gene="gcvH"
                     /locus_tag="PK736_04325"
     CDS             4636..5016
                     /gene="gcvH"
                     /locus_tag="PK736_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRC32650.1"
                     /translation="MNFPSELKYTKDHEWVKVDGDTATVGITDFAQSELGDIVYVEIE
                     TVGDTLSREATFGSVEAVKTVSDLFMPVSGEVLEINSGLDTNPEAVNTDPYGAGWMVK
                     IRMTNAAEVNDLLSAEAYKSLIGH"
     gene            5048..5428
                     /locus_tag="PK736_04330"
     CDS             5048..5428
                     /locus_tag="PK736_04330"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRC32651.1"
                     /translation="MFLKWLPSSNKYWPTYLWALFILLLCAIPGQDLPKVWWMELLSI
                     DKPVHATLFFVMTLLLYRNLTFLEKPFLTAITSSLIFSMAYGGALEIMQGAYFENRSA
                     DIYDFVANTTGAILACYFVYRKKI"
     gene            5809..11253
                     /locus_tag="PK736_04335"
     CDS             5809..11253
                     /locus_tag="PK736_04335"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32652.1"
                     /translation="MSNLINSAAAQGSNKPWYSGNLQLSHTLFRNKTEWYYVIVALFA
                     FGTITNAQPWKQLGMGAGGQIRALYIDDKGNGTSDFYVGSDVAGVWKTGSISNGSLND
                     FTKYNYEYISNHEIMRFVNKFYRSAYAGLSDYIFTCNQSGIHKIERNATNGNMQKIVD
                     IEESDVSDMHIASLNTVTSSHSLYFVTGDTRLDDDANVKATGVYDFYFTTITPAGDIN
                     TPIQGVNFTGLGGQRDVYCLYVNDKGTPDETDDEFLAGTENGLYMFNFGNLNFVTHSV
                     AATLVSPPLNSGAYSVSSITAFTPTQFVVTIYGATSPGIFLFSTTQPYWPENYSNGAV
                     FESSKVANSTIKYSATSNELKGFAKLQRLTSNAGYLVFLTEPDTKFGNPLAKYYTGIY
                     YAEADAGNSYKPKNSWANLEFDNSTNDYGWNNSRPCANINSCVLNTLNRLWVGKSGNF
                     FVTDNIINNAGSVWKQIYTTANTGCTTTIMSYSNRGMVNTVPNAIFPDPFQPTRIFNC
                     NYDRHLNVSNNYGRSFEQVTISNVSPGSSCFWIDDSYIDYSLCNITDPTFSDCFTITN
                     NPYDNQNFSLYTGFAQGYSTKKGVGVAYELNNGSNIITPFGGTVQVNVNAGEVCGDPI
                     KIVFNPALPNDKYCIYNMTFPPTDRHLFKYNVTTFAWDELNLFAGNEDIIDAVITNIG
                     DLVVINGNTNARGVHKFTWVSNVWTYIGTPINANILGVQLEPFLANGKTYLICKATGQ
                     TGGNVTVIDANTWTVLPVNVQCGTASMDAFNNLIGDDDRTSCIGISEAAYSIYISTAK
                     GNRTYLYKGSYNPTTGEVLPCWDEITGMPNKIPKYINTNRCTAAKENLYVSMRGLGEW
                     QYCPTPFTLTATNPTCNGGNNGTATAAINCDRYPYNYLWNTSATSSTLINLAAGTYTV
                     TVTDGYGATSTQSITLNSPCCGNIILSQTNFDNATTFGIGTYNLNNNVICKANITINT
                     ATINIAGNVSITVPFGFTLTIENGSNLSACTNMWQGIVNQGGTVIVTGSTISDAIIGI
                     ENLKNGNGAVNNNIFDKNYTSLSYNNGNYATTKGVSIWGNTFKSGYNLKAPYFGMRSS
                     NFIRLSYVTDITIGDVTKLENTFGVLGNYEADNGIYATTSSLKVYHNKFYGFNLNSNI
                     TLDAGNAIKCYTDMGVMWQLGPHTPTIFNALIGDKTITNSGNFFIQCKRAIDLVNQSA
                     TIDVNTITNNANTPITEIDFVQSIYVHEGTNFDVAIEKNEFINNDKDISIDILGNVQG
                     AVNIQNQNHFNNFPTNISKESGAIRASDYIANSEGVSYNIFNNKDASLALADQGIHTK
                     GDFGIKITGGYVTIQENSVYLDHITTPTNDVFGVRIENSITPQVTCNLARGESATTTA
                     KKIGMSTAFSYESHFDCNTTDKTQVGMEFLSDCDNSVITGNSFQYHDIGFRLGRGFNG
                     GWTSGIIGDQNFYLDGLVKKTFGNRFEGTNGAGGFTIASMHSENSYAGLQPNLGFRIW
                     LYTQGYFEPKPPYTVDNSQVIDASAFAPSFSPSITQYYSCPSNCQSLNKPKPVDDMEE
                     DVVDVEATDTTAITGALRIQNNSRGANIYNELSIDTMRRDTNIVLSNFYNQRKTANTG
                     KLADAEILVASLFENASSTNQLNTLLQSIVPQYEYEQNSLDILNLIIAKKGIINAANL
                     SNNERQAIEVIASQCPYKGGKAVYKARTMVWLYNPGAKFIDDELCTNGAYFRQTNSEN
                     NSSNLYADITVIPNPSNDLVTLYYFTDSKEKGKLKIYNETAQLIKSYPIFAEKNSIEL
                     SVQDLISGIYNIQIVFTDRPVQRTKLVVIK"
     assembly_gap    11317..11326
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3797 a         2181 c         2242 g         3307 t
ORIGIN      
        1 aatggtaaca atttaaaacc cgcagccgtt accgatgttg aattatcaaa acatgatcag
       61 cgcgaagtgc tggagcaaga actattcgac aggaataacg gaacacaaac tattcctcca
      121 ttacaagttt tgaatttggc ttattacccc actgagcgcg gtccgtataa tttagatgta
      181 gggcctactc aattctcgaa aggggttgct gccgatggat ccttgaacga ccccgcaagc
      241 agatggggtg gtattatgcg caggctggaa acaaacgatt ttgaagctgc caatgtggaa
      301 tatattactt tctggttgat ggatccgttt aatgaagata accccaatca ggcagacgaa
      361 ggcaagcttt attttaattt aggtcaaatt tcggaagaca ttttgcacga tggttttaag
      421 ctttacgaaa acggcctgaa atgtcccaac aatacattgc caattgatac ttgcgcttgg
      481 ggtatttacc ctttcaaccc taccaatcca acaaacgcat ttgataacga tcctgattgc
      541 cgcgcattac aagatgttgg ctacgatgga ttaccaacat cacaggaaaa atcattcttt
      601 accggttatt tgtcggcctt aggtgctttg catggtacgg catcgcaagc atatattaaa
      661 gcggaggctg accccagtag cgataatttc cagtatttcc gcggctcaaa tgaaccattg
      721 ctcagcaatc catccattac aagccggtac aaaaacttta atagtcccga tggcaattca
      781 cctacacctc aacagtctcc tgaggcatat tctactcagg caaccaattt ccctgaatcg
      841 gaggatatta accgagacgg taatatggat acttacgaaa actattttca atatgaaata
      901 aaagtaagta aagccgattt aaaagttggg agcaactaca ttaccgataa acgcgaagta
      961 caggttacaa cacaggataa gcgcaagcgc accatagctt ggtatcaata tcgtattccg
     1021 gtaaacgatg aattgcgaca aaaaattggc gatattcagg atttgcgctc cataggtttt
     1081 atccgtatgt ttatgaaagg attttccgca cctattgtaa tgcgttttgg ccgcttagaa
     1141 cttacccgcg gtgaatggcg caagtacacc aacaaattga taagccccgg tatttacatc
     1201 gacccaccaa ttgccggtaa tacatctttc gaaatttcgg cagtaaacat cgaagaaaac
     1261 aaaggcaaag ttccatgtaa ctatgttatt cctccgggta tcatacagga agtaaatgtt
     1321 ggctcggcac agttacaaag acttaacgag caatcgatgg tgttgcgtgt gtgtggattg
     1381 caggatggtg atgcccgtgc ggctttcaaa accctggagc tcgacttgcg gaattataaa
     1441 cacgtaaaaa tgtttgttca ttgtgaagat aatcttgcct gtaccgatga tttcgattgc
     1501 ggacccggcc ctgtacaaga caacgacctg gttttatttg tgcgcctggg taccgataac
     1561 agccagaatt attatgaata tgaaatgcca ttaaaagtaa caaactacag tggcactgca
     1621 ataaaggatg ccgataacat ctggaaatcg gcaaatgaat tagacatcga ccttgagaag
     1681 ttgttgaatg cgaagttatc gcgcggcaat gcgggagggg cattgcaggc ctatgttcct
     1741 tatgggcaag atggcgaccg gaatatttat gttgttggtt cgcccaatct tagcaatgtg
     1801 cgcactatta tgattggaat ccgcaaccca aagcaaggcg gtatagttaa ctttggtaaa
     1861 gatgatggcc aggccaaatg tgccgaggtg tgggttaacg agttacgcct caccgatttt
     1921 gatgagcgcg gaggctatgc agccaatgcg cgcattgcta caaagcttgc caaccttgga
     1981 aatcttactc tgtcgggaag tcgcaaaaca attggatttg gaggtataga gcaaaaagta
     2041 aacgaacgtc aacgcgaaga ttttgttcaa tacgatattg caactacact ggaacttgga
     2101 aaatttttcc ccgataaaat atctttatcc ataccatttt actacagctt tggacaaata
     2161 attgccaagc cacagttcaa cccattggat cctgatgtgc cttttcaaac ggcattaaaa
     2221 aacctggaag ccgacaaagc tgcagagctt aggcgaaata ccatcgatat gacgaaaagt
     2281 aaaagtataa gctttaccaa tgtgcgcaaa aataaaactg gtggcaaatc gcatatttac
     2341 gatattgaaa attttaatgt cagttattct tataccgaat tatatcatcg caacataaat
     2401 ttacaatacg atcttttacg aaaccatcgt gcttcgttgg gatataacta taacgcaact
     2461 gtaaaaccgt gggagccatt caagaaatcg aagtttgtag gaaactccaa gtggataaaa
     2521 ctggtaaagg atgtaaacgt aaatcctgtt ccaaacagtt taagtttccg caccgacctc
     2581 gaccgtcagt acggtgagca gttgttgcga aacaatacta cctacaaagc cattatcgat
     2641 actactttca ataaatactt taactggata agggcatacg atttacgttg ggatattacc
     2701 aaatcgataa agttcgattt ttcggcaaac aatcaggcac gtgttgacga gcctgcaggg
     2761 cgcatcgata cacaagaaga aaaagattct atcaaataca acgtaaggca gcttggccgt
     2821 aatacgcagt atggccacaa tggaaatgtg aactaccaga taccgttaaa taaaattcca
     2881 ttgctcgatt ggatttcggt taataccaaa tatggttttg attatggatg gcaagcagcc
     2941 cctttgaggt tcgatagtgt gttgaacaaa acggccccta atgcggcatt gggcaatacc
     3001 attcaaaact cgaatacaaa atcggtaaat gctacgctca cgttcactca actttataac
     3061 aaggtgccat ttttcaaaaa attattggcg cccaagcctc ccaaaccagt tacaccaaaa
     3121 gtgaagcccg agccaaaaaa agatcctaat gataccacga aagtaaaaac accacctccg
     3181 ccaaaacctc ccagaggaat acccgatgtg gtgcgcttta ttggtaaggc tattttttgt
     3241 gtaaaaagtg ccggtggaac atttagcgaa accaatggta ctatgtttcc cggatatcgc
     3301 aggcgtaccg aaatgctggg acaggaatat tataccaacg aaaaaggaga aagtgtaaca
     3361 gcaccgggat ggggttttgt ttttggcagc cagaaagata ttcgatttga tgcagcccaa
     3421 aacctttggc taagcagcga taccagtttc aattcgcagt acacacgcaa tttcacacaa
     3481 aacattaccg cacgcagcac tattgaacct ataaccggtt tccgtattga gttgaatgcc
     3541 acacgtaatt atgcccgcaa cctgagcgaa aactaccgtg caacgccatc gggtgtttac
     3601 cgttcggttg gtgcgctcga aacaggtaat ttcagcatat catacattac atggaatact
     3661 tcttttataa agccgaacaa ggacaatact tccgatgtat ttaaaacatt cgatgccaac
     3721 cgtcaaatta tttcacgcag aataggcgta ctaaatcctt actcgtcatg ggcagttgac
     3781 gattcgggct atgttgatgg atatagtgga acgcagcagg atgtactcac cttatcattt
     3841 ctggcagctt atagtgggcg caatcccgaa acaattaaga acgagcaatt tttaaaaatt
     3901 cctttgccta actggcgaat tacttacgat gggttgagca aaatggcgtg gtccaaaaaa
     3961 ttgtttacca gtgtaaatat tacccatggg tatcgctcca catacaacat caatacattt
     4021 aataccaacc tcaattatgc cgatccgcaa aattacggta caagtggcgt tcgcgatgcc
     4081 agcggcaatt ttataccaaa gtacgaagcc actcaggtga gcattaccga atcgtttagt
     4141 ccgctcattg gtgttgacat gacctggaaa aagaatggtt tatttacacg catggaattg
     4201 aaacgcgatc gcaatttgtc gctcacttat accggtattc aacttaccga aatacggtcg
     4261 aatgaactta cagtaggtgc aggttacaga attccgaaat ttaaacttcc atttaaaata
     4321 agaggaaaac aagtaaaatt gcaaaatgat ttgaatatta cagccgattt tggaatgcgc
     4381 aaaaacataa ctgttattag aaaaatgcag gagaaaacag atattcctac ttcgggtatg
     4441 aattcctaca ctataaaagt tgctgccgat tatgttatta atgatcgatt taatgtacgt
     4501 ttctatttcg ataaagtaat aaacgaaccg gtaattgcta catcgtttcc caactcgaat
     4561 actcaggttg gtttcgccat ccgatttaca cttgcacaat aaggatttta aaaaaaataa
     4621 ttaaaaaatt gaattatgaa tttcccctca gaattaaagt ataccaaaga ccacgagtgg
     4681 gttaaggtag atggcgacac ggccacagtt ggaattaccg attttgcaca aagcgaattg
     4741 ggcgatattg tttatgttga gattgaaact gtaggcgata cacttagtcg cgaagctact
     4801 ttcggcagtg tagaagctgt aaaaacggta tccgatttat ttatgccggt aagtggcgaa
     4861 gtactggaaa taaattccgg tctcgatact aatcccgaag ctgtaaatac agatccttac
     4921 ggtgcaggat ggatggtgaa aataagaatg accaatgctg cagaagtaaa tgatttatta
     4981 tcggccgagg cctacaaaag tctgataggc cactaactac gtatgtagta atagttagta
     5041 attttttatg tttttaaaat ggctgccatc tagcaataaa tactggccca catatttgtg
     5101 ggctttgttt attttactgc tctgtgctat tcccggacaa gacttgccaa aggtgtggtg
     5161 gatggaactt ctcagcatag acaaaccggt acatgccaca ctcttttttg tgatgacgct
     5221 tctcctttat cgaaacctaa cttttttaga aaagccattt ctcactgcaa taacaagctc
     5281 attaatattt agtatggcct acggtggcgc gctcgaaata atgcagggcg cttatttcga
     5341 aaatcgcagt gccgatattt atgattttgt ggcaaatact accggagcca ttttagcgtg
     5401 ttattttgtt tatcgcaaaa aaatatgatt ttggttttta ttttttataa ttaatattgc
     5461 gatacttttt aacaaaacat tctccctttg gttgggctag tcaaaaccaa aatcaatgat
     5521 tttaaaccaa tgcaaaaatt tttcttaagc ttcttatttt cgaatattat taggttgatg
     5581 gtttttttgt gagatgcgtt tggttgccat tttgactaac caaatatggc tgagtaaaac
     5641 cagatgaaaa ctttttcttg ccccaatcat tttgttttct acatttacac tgaaataaaa
     5701 cattgctttt gctaaatcaa aattgattga ctcaatagtt gtacataaat aatttgttta
     5761 taaataaatg gtactaatga tatatttaaa ctaaaaaaaa ataattttat gtcaaactta
     5821 attaacagcg cagcagcgca agggagcaac aagccatggt acagcggtaa tcttcaatta
     5881 agccatactt tatttcgaaa taaaacagaa tggtattatg taattgtagc acttttcgct
     5941 tttggtacta ttacaaatgc acaaccatgg aaacaattag gtatgggtgc cggagggcaa
     6001 atacgtgctc tatatataga tgataaagga aatggtacca gtgactttta tgttggcagt
     6061 gatgttgccg gagtttggaa aactggtagt atatcaaatg ggtcattgaa tgattttaca
     6121 aaatacaatt atgaatacat cagcaatcat gagataatga gatttgtaaa taaattttac
     6181 cggtcagctt acgcagggtt gagcgactat atttttactt gtaatcaatc cggcatccac
     6241 aaaattgaaa gaaatgcaac aaatgggaat atgcaaaaaa ttgttgatat tgaagaatcg
     6301 gatgttagcg atatgcacat tgcatcatta aatactgtaa caagttcgca ctcactatat
     6361 tttgtcacag gtgatacaag acttgacgat gatgctaacg ttaaagcgac aggagtttat
     6421 gatttttatt ttactaccat tacaccagcg ggcgatatta atacaccaat acaaggagta
     6481 aattttacag gactcggtgg tcagcgcgat gtatattgtt tgtatgtaaa tgacaaagga
     6541 acgcccgatg aaactgatga tgaattttta gccgggacag aaaatggctt atatatgttc
     6601 aattttggca atctaaattt tgtaactcat tctgttgctg caacacttgt ttcaccacca
     6661 ttgaacagcg gtgcatatag cgtgagtagt attacagcat ttacgccaac ccaatttgtt
     6721 gtcacaatat acggggcaac ttcgcccggt atttttttat tcagcaccac tcaaccttat
     6781 tggcccgaaa attattcgaa cggtgcagta tttgaatctt ctaaagttgc aaattctact
     6841 attaaataca gtgctacaag taacgaacta aaagggtttg caaaacttca acggttaact
     6901 tcaaatgccg gttatttagt tttcttaact gaaccagaca ccaaatttgg taatccttta
     6961 gcaaaatatt acactggaat atattatgct gaggctgatg ccggcaatag ttacaaaccc
     7021 aaaaattcgt gggcaaattt agagtttgac aattcaacga atgactacgg atggaataat
     7081 tcgcgaccat gtgcaaatat taatagctgc gtattgaata cattaaaccg attgtgggtt
     7141 ggtaaatccg gcaatttttt tgttaccgat aatattatta acaatgctgg cagtgtatgg
     7201 aagcaaattt ataccactgc taacaccggc tgtaccacca ccattatgag ctattcaaac
     7261 agaggtatgg taaatacggt tcctaacgcc atttttcccg atccttttca gcccactcgt
     7321 atttttaact gtaattacga taggcactta aatgtttcta acaactacgg gcgaagtttt
     7381 gagcaagtaa ctatcagcaa tgtttcgccc ggttcatctt gcttttggat tgatgattcg
     7441 tatattgatt attctttatg caacattaca gaccctactt tttccgactg ttttaccatt
     7501 acaaataatc cgtatgacaa tcaaaacttt agtctctata caggttttgc acaaggatat
     7561 agtaccaaaa aaggcgttgg agttgcttat gaattgaata atggctctaa cattattacg
     7621 ccttttggtg gcacagttca agtaaatgtt aatgcaggtg aagtatgtgg tgatcctatt
     7681 aaaatagtat ttaatccagc attaccaaat gacaagtatt gcatttacaa tatgacattc
     7741 ccgcctacag ataggcactt atttaagtac aatgtaacta catttgcatg ggatgagtta
     7801 aatttatttg ctggcaacga agatattatt gatgcagtaa ttacaaatat tggtgacttg
     7861 gttgttataa atggcaatac aaacgccaga ggtgtgcata agtttacatg ggtaagcaat
     7921 gtgtggacgt acatagggac accgataaat gccaatatac ttggtgttca gttagagccc
     7981 tttttggcaa atggaaaaac ttatctaata tgtaaagcaa ccggacaaac aggcggcaat
     8041 gttacagtaa ttgatgcaaa tacctggact gtattaccgg taaatgtaca atgcggcact
     8101 gccagcatgg atgccttcaa caacttgatt ggcgatgatg acagaacttc ttgtattgga
     8161 atatcagaag cagcctactc aatctatatt tctacagcaa aaggaaatcg tacttatctt
     8221 tataaaggaa gctacaatcc aactaccggt gaagtattgc catgttggga cgaaataacc
     8281 ggtatgccaa acaaaatacc aaagtatata aataccaacc gctgcacagc tgccaaagaa
     8341 aacttatatg tttctatgcg tggacttggc gagtggcaat attgccccac accatttaca
     8401 ctcacagcca ccaacccaac ttgtaacgga ggcaataatg gtactgcaac tgctgcaatc
     8461 aattgtgacc gctaccctta taattattta tggaatacca gtgctacttc cagcacatta
     8521 attaatcttg cagctggtac gtatactgtt accgttactg atgggtatgg agcaacctcc
     8581 acacaaagca ttacgctaaa cagcccttgt tgtggcaata ttatactgag ccaaacaaac
     8641 tttgataacg ccaccacatt tgggataggc acctataatt taaataacaa tgttatttgc
     8701 aaggccaata taactattaa cacggcaact attaatattg ccggcaatgt tagtattaca
     8761 gttcctttcg gctttacttt aactattgaa aatggcagca atttgtctgc ttgtactaat
     8821 atgtggcaag gtatagtgaa ccaaggcggt accgttattg tcacaggctc aaccatcagt
     8881 gatgcaatta ttggcattga aaacttaaaa aatggaaatg gcgcagttaa caataatatt
     8941 tttgataaaa actatacttc attaagctat aataatggaa attatgctac tacaaaaggc
     9001 gtaagtattt ggggtaatac ctttaaaagc ggctataact taaaagcacc atactttggt
     9061 atgcgctcca gtaactttat aagattaagt tatgttacag atattacaat tggtgatgta
     9121 acaaagcttg aaaacacgtt tggtgttttg ggaaattatg aagctgataa tggcatttat
     9181 gcaactactt cttcgctcaa agtatatcat aataaatttt atggatttaa ccttaactca
     9241 aacataacac tggatgcagg caatgcaata aaatgctaca cagatatggg tgtaatgtgg
     9301 caattaggcc cacatacacc aactatattt aatgcattaa ttggagacaa aacaattaca
     9361 aacagtggca acttttttat acaatgcaag cgggcaattg atttagtaaa ccaaagtgca
     9421 acaatagatg taaacactat taccaacaat gcaaacacac ctattactga aatagatttt
     9481 gtgcaaagta tatatgttca tgaggggact aattttgatg tagcaattga aaaaaatgaa
     9541 ttcatcaata atgataagga tataagtata gatattttag gaaatgtaca aggtgcagta
     9601 aatattcaaa accaaaatca ttttaataac ttccccacta atatatcaaa agaatcaggt
     9661 gctatacgtg caagcgatta tattgccaac agcgaggggg taagctataa tatttttaat
     9721 aacaaagacg cctctcttgc acttgccgac caaggcatac acacaaaagg cgattttgga
     9781 ataaaaatta caggcggcta tgtaaccatt caggagaaca gtgtttacct cgaccatatt
     9841 actactccta caaacgatgt gtttggtgta agaatagaaa atagcatcac accacaagtt
     9901 acctgcaacc ttgcaagggg agaatctgca acaacaaccg caaaaaaaat tggaatgagt
     9961 acagccttta gttacgaaag ccattttgat tgtaatacta ccgataaaac ccaagtagga
    10021 atggaatttt tatccgattg cgataactca gtaattaccg gaaatagttt tcagtaccac
    10081 gatataggat ttaggttagg aagaggtttt aatggcggat ggacatcagg cataataggc
    10141 gaccaaaatt tttatttaga tggtttagtg aaaaaaacat ttggcaaccg atttgaagga
    10201 actaatggtg caggtggttt tactatagca agtatgcata gtgaaaattc ttatgcaggg
    10261 ttgcagccaa atttaggttt tcgcatatgg ttatatacac aaggctattt tgagcctaag
    10321 ccaccttata ctgttgataa tagccaagtg atagatgctt cagcttttgc tccttccttt
    10381 agcccatcta taactcaata ctatagctgt ccatccaatt gccaaagcct caacaagccc
    10441 aaaccagttg atgatatgga agaagatgtg gtagatgtag aagccacaga tactactgcc
    10501 attaccggtg cactgcgcat acaaaataac agccgtggtg caaatatcta taatgaacta
    10561 agtattgata ccatgcgtag agatacgaat attgtattat ctaattttta taatcaacgt
    10621 aaaactgcca atactggcaa acttgccgat gccgaaatac tggtagcttc gttatttgaa
    10681 aatgcctcaa gtactaatca attgaatacg cttttacaaa gtattgtacc gcaatatgaa
    10741 tacgagcaaa acagccttga tattttgaat ttaataattg cgaaaaaagg aattattaat
    10801 gctgcaaatt taagcaacaa cgaaaggcaa gcaatagaag tcatagccag ccaatgccct
    10861 tacaaaggtg gtaaggcagt ttataaagcc cgcaccatgg tatggttata taatccagga
    10921 gcaaaattta ttgatgatga actttgtact aatggagcgt atttcagaca aactaattct
    10981 gaaaataata gttcgaattt atatgcagat attactgtaa tcccaaatcc atcaaatgac
    11041 ttggttaccc tatattattt cacggatagt aaagaaaagg gcaaattgaa aatatataat
    11101 gaaacagccc aacttattaa aagctatcca atttttgctg aaaaaaattc cattgaactg
    11161 tctgtacaag atttaatttc tggaatatac aacatccaaa tagtttttac tgacagacca
    11221 gtacaacgaa ctaaacttgt tgttattaaa taattagatg ggggttgtat agcaaccccc
    11281 atctaattat ttaataacaa caagtttagt tcggtgnnnn nnnnnnacaa aaaaatactt
    11341 tattttttta atagcaggtt ttgccgcaca tttttatgta ttggctcaaa acagggatgc
    11401 agcatgggaa cttggttttg gctgcggaaa tggttcatat ccaatgccac aatttcaatt
    11461 cataaacggt ataatagata cttcccgctg tattggaccg attggttttt tggtaaccac
    11521 ccctagtatt ggcgatc
//
LOCUS       DAORQA010000043        11522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1522_length_11522_cov_10.069329, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000043 DAORQA010000000
VERSION     DAORQA010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11522
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1522_length_11522_cov_10.069329"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(382..1710)
                     /locus_tag="PK736_04340"
     CDS             complement(382..1710)
                     /locus_tag="PK736_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRC32653.1"
                     /translation="MRKIIMFGAGRSATAAIHFFLDNALQNNWHLYLADADLNSAQQK
                     INGNPNGTAIELDINDEPARAKIIAEADIVISLLPPALHLLVAQDCIAHKKNLVTASY
                     ISPEIKAMNNDAVANGVLLMNECGLDPGLDHMSAMHIIDTIKSNGGRIRSFKSYCGGL
                     VAPESNDNPWGYKFSWNPRNVILAGQSTAKFIENGKFKYIPYTRIFADIEKIEVPGLT
                     SFDGYANRDSLGYLELYGLDGIDTMLRGTLRYEGYCKAWNIFVALGLTDDNCTIDGLS
                     KMTYAQLLESFLPRISKSKNIYEIIADVSHLEKHDDAINKFLWTGIASDLKIETTGYT
                     PAAALQSLLEQKWKLQPHDKDMVVMQHQFEYTTADNESKQIFSDLVIKGDDNINTAMA
                     KTVGLPLAVTVQMILEKKINLTGVHLPVMKQIYEPVLNKLKEYNIVFVER"
     gene            1884..3770
                     /locus_tag="PK736_04345"
     CDS             1884..3770
                     /locus_tag="PK736_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HRC32654.1"
                     /translation="MKAKFNLINIPRKFILVIDLAICTVSVLVAYQLRFNFAVPTREW
                     EIFPLALFSILVARSISLLGFKIYRSSIRFSGSSDVFKITGALACSALMVFAANLVSY
                     WFREKYLAPKSIIIIEFLVSTFVMVLGRMSIRALYNEMMNPTSQKSKVIIFGAGRSGQ
                     IAKRSLDRDAGSRFKVFAFVDDNKQLDKLIIEGVPVYNTFDKLEKLLSDNEIKHLIIA
                     IQNLGARRKAEVAGICLKYNTKVLDVPPVKSWINGELSFKQIKKIKVEDLLERDSIVL
                     DIEIIKAQLHQKTILITGAAGSIGSEIVRQLLTFEPQHVVMVDNAESALYDIEMEILD
                     MQLQNIKVTRLLADIRHKESMTRIFEYHKPQIVFHAAAYKHVPMMEENVHEAINTNVV
                     GTKILADCAIANGVEKFVMVSTDKAVNPTNVMGASKRIAEIYCQTNNNVSNTKFITTR
                     FGNVLGSNGSVIPLFKKQIEEGKSIKVTHPEITRYFMTIREACQLVLEAGAIGNGGEI
                     FVFDMGTPVKIADLAKKMITLYGLKLGRDIEINYTGLRPGEKLYEELLNDNESVLPTH
                     HSKIMKAKVPEYDYESVTKNMMELIAINNGEDTMAIVQKMKSMVPEYISNNSEFEILD
                     KKTN"
     gene            3841..3929
                     /locus_tag="PK736_04350"
     tRNA            3841..3929
                     /locus_tag="PK736_04350"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3875..3877,aa:Ser,seq:gct)
     gene            4008..4081
                     /locus_tag="PK736_04355"
     tRNA            4008..4081
                     /locus_tag="PK736_04355"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4042..4044,aa:Pro,seq:tgg)
     gene            4170..4243
                     /locus_tag="PK736_04360"
     tRNA            4170..4243
                     /locus_tag="PK736_04360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4204..4206,aa:Arg,seq:tct)
     gene            4279..4398
                     /locus_tag="PK736_04365"
     CDS             4279..4398
                     /locus_tag="PK736_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HRC32655.1"
                     /translation="MGKGDPKSKKGKIILGSFGKRRPARKQNEATAKPKIAKK"
     gene            4680..5642
                     /locus_tag="PK736_04370"
     CDS             4680..5642
                     /locus_tag="PK736_04370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRC32656.1"
                     /translation="MKILVIQTAFIGDAILATALLESLHHWNSKASIDFMVRSGNESL
                     FEEHPYINHLYIWHKPKNKIANLVKLAFDIRKQKYDLVVNLHRFASSGFVSFMSGAKD
                     KRGFDKNPLAFFYNKKVIHDLRVGLHETERNHLLIADIVEAKSSKPKLYPSPKIFDSI
                     KHLQTENYVCLAPASVWFTKQMPQVKWEQLAKKLSASTLVYLLGAPGDLKLCNAIAAN
                     VVHKNVIVLAGKLSLLQSAALMNSATMNYVNDSAPMHLCSAMNAPVTVFFCSTIPQFG
                     FGPLSDNSKIVEATTPLDCRPCGIHGFRACPQQHFKCGNDIEIL"
     gene            5643..6236
                     /locus_tag="PK736_04375"
     CDS             5643..6236
                     /locus_tag="PK736_04375"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRC32657.1"
                     /translation="MEQRNTPTTDYTADLNDALNALKTGQTILYPTDTIWGLGCDATN
                     ESAVQKIMAIKNRDAEKSFIVLVSDLDMLCRYVDVIPQPAITLMEYAEKPLTIIYDKV
                     SGLAESVYASDGSVGIRWCKDAFCQALIRKLKKPIVSTSANFSGMVSPSNFSEVSPQV
                     IEAAGYVVKHRQREKSKNTASTIFKIKDNGQLIVIRK"
     gene            6629..8053
                     /locus_tag="PK736_04380"
     CDS             6629..8053
                     /locus_tag="PK736_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRC32658.1"
                     /translation="MKFNKINEDKVLRTVSETAAEMHLQAYVVGGWVRDCLMHRPSTD
                     IDIVCDGDATLLATTVAKKLHENIKVSIFKNFGTAHFIYNNIDYEFVGARKESYREHS
                     RKPEVVPGTIEDDQLRRDFTINAMAISLQKKNMGELIDPFHGVDDIENRIIRTPLDPE
                     ITFSDDPLRMMRAIRFASQLQFTIEQITYEGIKSQVERIRIVSFERIIDELNKIILSP
                     QPSRGFVLLLNTGLLNIIFPEMCALQGAEYKDGKGHKDNFYHTLEVLDNISLNTEDLW
                     LRWAAILHDIAKPLTKRFEPGQGWTFHGHEDKGARMVPRIFAKLKLPLNEKMKFVQKL
                     VALHLRPIVLAQEIVTDSAVRRLLFDAGEEIDALMLLCEADVTTKNEYKKKKYKENFE
                     LVKLKLIEVEEKDRIRYWEPPISGEEIMKTFNLPPGRIIGIIKNHIRESILDGVIPNE
                     HEPAYLLMLAKAAQLGLTPVSIIK"
     gene            8145..8780
                     /locus_tag="PK736_04385"
     CDS             8145..8780
                     /locus_tag="PK736_04385"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32659.1"
                     /translation="MASYKFRITFEEYDDITRDIEIKSTQTFLDLHNCLQESIKFDNS
                     KPASFFMSNDRWIKGTEISLNPKTLKDGTDVALMKTAVMNKYIYDPHQKIYYEFGEWC
                     FFVELIKINTTDDKNPYPRCVKVNGEAPKQYKVVKAIPGVMELNNLELPEGEPEPDED
                     DIEEIETADAGIVEGIDEGETEIGFGIESDSEDEIPEEEMDENFGNDHEEI"
     gene            8819..9703
                     /gene="miaA"
                     /locus_tag="PK736_04390"
     CDS             8819..9703
                     /gene="miaA"
                     /locus_tag="PK736_04390"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRC32660.1"
                     /translation="MPTLIVICGPTASGKTQVAISIAQQLSAPIINFDSRQFFRELNI
                     GTAKPTPEQLVQAKHFFIDSHSITDEYNAVAFGNDAQKLIDELSHQHQYIILCGGSGL
                     YLNAVLFGMDAMPEKSKSIRQTINKNYEQFGLSYLQEEVKKADAKYYETVDTENPHRL
                     IRALEVYMQTGKPFSDFRNGQNKNSAYDYKLCGITLPREQLYERINQRTDMMIAQGLE
                     NETRQVIDYRKHHALQTVGYKEMFQYIDGEISLQQTIDLIKQHTRNYAKRQITWFKKY
                     DNINWYAPNEVDKIMAAL"
     gene            complement(9851..10852)
                     /locus_tag="PK736_04395"
     CDS             complement(9851..10852)
                     /locus_tag="PK736_04395"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRC32661.1"
                     /translation="MNLKSIVLYLLYATVGGLLIYLFLQNAPLTEIKNEIAKANLKWY
                     GVVIMVSIAVYVVRTLRWQMMLRSINCRPHFVETFAAISTSYFVSIGVPRFGEITRCI
                     AIARRRKVPFVAVLGTVITERIIDVLALFIVLIFTFMVQNAEMRIFYLNNIITPIKQS
                     LGVKFDTGNLKILIILLAVIAVGWLLMFLLQRGFKRLPQSVQNIFIEAKNGLTSINKL
                     KHAWLFYLYTLLIWVGYFFMTWFWFFAFDETRNLGWGVCLSIISIGTIGRSVPIQGGG
                     MGAYHYLVEKVTAVYNISATMGKTLAIVIHGGQIIFTLCIGILGWLYLVFSNPQKIK"
     gene            complement(10885..11289)
                     /locus_tag="PK736_04400"
     CDS             complement(10885..11289)
                     /locus_tag="PK736_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRC32662.1"
                     /translation="MEKQITTVRIDKWLWCVRIFKTRSMASQYCDKGKIKIKGDVIKP
                     SRNVKPGDIVTFQDGALIRTFEVLMLLDSRVGAKDVFKYCKETTPEETLLQHAALRKA
                     AAAWRMPGAGRPTKKERRELDDFIWGEDIGEI"
BASE COUNT         3800 a         2197 c         2193 g         3332 t
ORIGIN      
        1 gtccctagct ttctactgca taatttaggt ttagtaaaat gaaaatagaa actggtgtta
       61 aaaaaaacca gtgtaggcaa ctttgatgca aattatacaa ttgaaattat cctttacaca
      121 atgattaata aaatctattc ttttggatct atggtacctc accaaatggg agattctcta
      181 aatcaggatt aaccgaacct atgtatatag ttcggctggt tccacacttt cttctttata
      241 attctgatta aaaatgacca tccccattta gtggccgaga tgcttcgcaa ttcgagaata
      301 ctcattgtga caaatcgcaa tgaagataat gaccaatgac aattcctcag catgtcaatt
      361 ccagcaacct aataaccaaa attaacgctc aacaaacaca atattatact ccttcagttt
      421 attaagcacc ggttcgtata tttgtttcat taccggcaag tgcacaccag tgaggtttat
      481 ttttttttct aaaatcattt gaactgtcac agctaaaggc aaacctactg ttttggccat
      541 agcagtattt atattgtcgt caccttttat caccaggtcc gaaaatattt gttttgactc
      601 attatcagca gtagtgtatt caaactgatg ttgcattact accatatcct tatcgtgtgg
      661 ttgtaatttc catttttgtt ccagcaaact ttgcaatgca gctgcgggag tataaccggt
      721 tgtttcaatt tttaaatcgg aagcaattcc tgtccacaaa aatttattta ttgcatcatc
      781 atgcttttcg agatgggata catcagctat aatttcatag atattttttg atttagaaat
      841 gcggggtaaa aaagattcga gcaattgggc ataagtcatt tttgataagc catcaattgt
      901 gcaattatca tcggtgaggc caagggcaac aaaaatattc catgctttgc agtagccctc
      961 ataacgcaag gtaccacgca acatagtatc aataccatcg aggccataca attcaaggta
     1021 tccaagcgaa tcgcggttgg catatccatc gaaagatgta agaccgggca cttcaatttt
     1081 ttctatgtca gcaaaaatac gtgtgtacgg aatgtacttg aactttccat tttcaataaa
     1141 cttggcagtg ctttgtccgg caagaattac attgcgtgga ttccacgaaa atttgtaacc
     1201 ccagggattg tcgttgcttt cgggagctac cagtcctcca caataagatt taaatgaacg
     1261 tatacggcca ccgttacttt ttatggtatc gataatatgc atggcactca tgtggtcaag
     1321 ccccgggtcg aggccacact cattcatcag taaaactcca tttgcaactg catcattatt
     1381 cattgctttt atttcgggag agatgtatga tgccgttaca agattttttt tatgtgcaat
     1441 gcaatcctgt gctactaata aatgtaaggc cggtggtaat aaagaaataa caatatctgc
     1501 ttcggcaatt atttttgccc gtgccggttc atcattaata tcaagttcta ttgctgtgcc
     1561 atttgggttg ccatttattt tttgctgggc cgaattcaaa tcggcatcgg caaggtacag
     1621 gtgccagttg ttttgcaatg cattatcgag aaaaaaatgt atagctgcag ttgccgaacg
     1681 tccggcccca aacatgatta ttttgcgcat aatattttta cagatttaaa gcacaaagta
     1741 acaccataca tttcgaataa taaaacagca acagacatat acctgcttaa aaacctgaaa
     1801 agcaaaacat caaaaaaagg aacaaaaaaa ataaggacct ttttactaac tcatatttta
     1861 gtaatattgt tgcccttatt aaaatgaaag caaagtttaa tctcataaat attccccgta
     1921 aatttatcct tgttatcgac ttggctattt gcacagtttc ggtgttggtg gcatatcagc
     1981 tgcggttcaa ctttgcagtg ccaacgcgcg aatgggaaat ttttcctttg gccttatttt
     2041 ccatactggt agcacgaagc atatctttac tgggatttaa aatttaccgt tcctctatca
     2101 gattttcggg ctcgagcgat gtatttaaaa ttaccggggc tttggcttgc agcgcgctga
     2161 tggtttttgc tgctaattta gtttcttatt ggtttcgcga aaaatatctt gcgcccaaat
     2221 ctatcatcat catcgaattt ttggtttcca cttttgtgat ggtgttaggc cgcatgagta
     2281 taagggcatt gtacaacgaa atgatgaatc caacttcgca aaaatcgaaa gtgataatat
     2341 ttggtgccgg ccgttcaggg caaattgcga aacggtcgct cgatcgcgat gccggttcgc
     2401 gatttaaagt ttttgctttt gttgacgata acaaacaact tgacaaactc attatagaag
     2461 gcgtaccggt atacaatact ttcgacaagc tggaaaaatt attgagcgac aacgaaataa
     2521 aacatttgat aattgctatt caaaatttag gagcaaggcg caaagccgaa gtagcaggca
     2581 tttgcttaaa gtacaacacg aaggtgcttg atgtgccacc cgtaaaaagc tggattaatg
     2641 gcgagcttag ctttaagcaa ataaaaaaaa taaaagtgga ggatttactc gagcgcgatt
     2701 ccatcgttct cgatatagaa attatcaaag cacagttgca tcaaaaaacc attctcatta
     2761 caggtgctgc cggaagcata ggaagtgaaa tagtacggca attactcacc tttgagccac
     2821 agcatgttgt tatggtagac aatgccgagt cggccctgta cgatattgaa atggaaatac
     2881 tcgacatgca attgcaaaat ataaaagtaa cgcgcctcct tgctgatatc cgccacaaag
     2941 aaagcatgac gcgaattttc gaatatcata aaccgcaaat tgtttttcat gcagcagcgt
     3001 ataaacatgt tcccatgatg gaagaaaatg tgcacgaggc tattaataca aatgtggtgg
     3061 ggacaaaaat tcttgccgat tgtgccatag caaacggtgt agaaaaattt gtaatggtat
     3121 cgaccgataa agctgtaaac ccaaccaatg tgatgggtgc aagtaagcgc attgccgaaa
     3181 tttattgcca aaccaacaac aatgtttcca atacaaaatt cattaccacc cggtttggaa
     3241 acgtgttggg cagcaatggt tcggtaattc cactattcaa aaaacaaata gaagaaggta
     3301 aatccattaa agtaacacac cctgaaatta cacgctattt tatgaccatt cgcgaagcat
     3361 gccaactggt gctggaagcg ggagctatag gtaatggtgg cgaaatattt gttttcgata
     3421 tgggtactcc tgtgaagatt gccgacctgg caaaaaaaat gataaccctt tatggattga
     3481 aactcgggcg tgatatagaa ataaattata ccggattgcg tcccggagaa aaactatacg
     3541 aagaactgct taacgacaat gaaagtgtgc tgccaacaca tcattcaaaa attatgaaag
     3601 ccaaagtgcc ggaatatgat tatgaaagcg taacgaaaaa catgatggaa ttaattgcga
     3661 ttaataatgg cgaagacaca atggctatag ttcagaaaat gaaaagcatg gttcccgaat
     3721 atatcagcaa caactcggag tttgaaatat tagataaaaa aacaaactaa attatttgtt
     3781 ttggaaaaga caatgtagtt tttacctttg cgcgctcgaa aaaagggctg ataggattga
     3841 ggtgaggtgg atgagtggct gaagtcaaca gtttgctaaa ctgtcatacg gagaaatctg
     3901 tatcgagggt tcgaatccct ccctcaccgc aaactaaagt tttgcattgt ctttgaaata
     3961 tcaatttgct tcgaaaagtg ggaaactact aatggtaatg agttcttcgg ggtgtagcgt
     4021 agcccggtat cgcgcctgct ttgggagcag gaggtcgtag gttcgaatcc tgccaccccg
     4081 acacacagat aaaaatcccg caattgcggg attttttttg tctgcaaaaa gttatcattg
     4141 aaaaagcaaa ttgctatctt tgtacatgcg gtctcgtagc tcagctggat agagcaactg
     4201 ccttctaagc agtaggtcgc tggttcgagt ccagccggga tcactctaaa aataggtatt
     4261 aataaaacta aaataaaaat gggaaaaggc gatccaaaat caaaaaaagg taagattatt
     4321 cttggctctt ttggcaaacg cagaccggca cgtaagcaaa acgaagccac tgcaaaacca
     4381 aaaatagcga agaagtaatt cactaatttt tactaatcga aatgtgcaaa gcccgcagct
     4441 aaatgttgcg ggctttttag tttgtgtttt ttttcactca ttcaccgcca aatttttata
     4501 gtttgttttt taaactgaac accatcatta taaaaaataa tttttgcgat gatggaattc
     4561 aaatcctcaa ttctaaaagt aaaacatcaa cttatcactt cggtttatat cctgcttttc
     4621 gattgtatcc cgtaacaaaa tcaaaacatt acatttgcca tcaatatcga taacctcaat
     4681 tgaaaatact tgtaatacaa acggccttca ttggcgatgc tatactggcg actgctttac
     4741 tggaatcgct gcaccattgg aacagcaaag catcgattga ttttatggtt cgtagcggta
     4801 atgaaagttt gtttgaagaa catccataca tcaatcattt atacatttgg cataaaccta
     4861 aaaacaaaat tgcaaacctt gtaaaacttg catttgatat tcgaaaacag aaatacgatt
     4921 tggtggtaaa tttacatcgc tttgcatcat ccggatttgt gagtttcatg agtggtgcca
     4981 aggataaacg agggttcgat aaaaatcctt tagcattttt ctataataaa aaagtaatac
     5041 acgacttaag agttggtttg cacgaaacgg aacgcaatca tttgcttata gccgacatag
     5101 ttgaagcaaa atcctcgaaa ccaaaattat atccgtcacc aaaaatattt gacagcataa
     5161 aacatttgca aacggaaaac tatgtttgcc ttgcacctgc ctcggtatgg tttactaaac
     5221 aaatgccaca ggttaaatgg gaacaactcg caaaaaaatt atcggcaagc acacttgttt
     5281 atttgttggg tgcacccggt gatttgaaac tatgcaatgc tattgctgca aatgttgtgc
     5341 ataaaaatgt gatagtgctt gccggtaaat tatcgctgtt acaatcggct gcacttatga
     5401 atagtgcgac aatgaattat gtaaacgatt cggcacctat gcacctatgc tcggcaatga
     5461 atgcaccggt tactgttttc ttttgcagca ccattccgca atttgggttt ggtccattat
     5521 ccgataacag taaaatagta gaagctacta cccctctcga ttgcaggccc tgtggcattc
     5581 atggttttcg cgcctgccct caacaacatt ttaaatgtgg aaacgatatt gaaatactat
     5641 aaatggaaca aagaaacaca ccaacaacag actacaccgc tgacttaaat gatgcattaa
     5701 atgcattgaa aacagggcaa actatattgt atccaaccga tactatttgg ggattgggtt
     5761 gcgatgctac aaatgaaagt gcggtacaaa aaataatggc cataaaaaac cgtgatgccg
     5821 aaaaaagttt tatcgttttg gtgagcgacc tcgatatgct ttgccgctat gttgacgtga
     5881 tacctcagcc tgctattaca ttgatggagt atgccgaaaa accactgaca attatttacg
     5941 ataaagtgag tggccttgcc gaatcggttt atgccagtga tggcagcgtg ggtatacgat
     6001 ggtgtaaaga tgctttctgt caggcactaa tacgcaagtt gaaaaaacct attgtctcta
     6061 cttctgccaa tttttcgggc atggtttcac cttccaattt ttcggaagtt tctccacaag
     6121 taattgaagc cgcaggctat gttgtaaaac accggcaacg cgaaaagtca aaaaatactg
     6181 catctactat tttcaaaata aaagataacg ggcaattgat agttattcga aagtaatgtg
     6241 atactgaaaa ataattgaaa cttaaaaatt tgagccctct actaaatgtc taattaaata
     6301 aagagccact aattcaccaa aacactaaat cccacaaaaa ataaaaaaag gtttgtttac
     6361 gacttagtga tattttgtga ctttgtgttt ttgtggcaaa aataactttt gggtgtagac
     6421 tcacatttct aatagagctt ttgtttactt cataaactta ttatcaacat gtgaaacaaa
     6481 cgcgaaactt ttttaccaaa tcagcaaggc aaaacaaatt ctcgtgtcca ttttcgttac
     6541 actctatagt ttgtaacata ctttttttct ccttaaataa gcattctcat ctctgagtaa
     6601 atcttaactt tgccgcacca attcaattat gaaattcaat aaaataaacg aagacaaggt
     6661 attaagaacc gtttcggaaa ctgcagcaga aatgcacctg caagcttatg tagttggcgg
     6721 ttgggtgcgc gattgcttaa tgcaccgtcc ttctaccgat attgatatag tttgcgatgg
     6781 cgatgcaacc ttacttgcta caacggtggc aaaaaaactt cacgaaaaca taaaagtaag
     6841 catattcaaa aattttggta cagctcattt tatatacaat aatattgatt acgagtttgt
     6901 aggtgcgcgc aaagagagct atcgtgaaca ttcgcgcaaa ccggaagtag taccgggcac
     6961 tattgaggac gatcagcttc gcagagattt taccatcaat gcaatggcaa tatcattaca
     7021 aaaaaaaaac atgggcgaac tcatcgatcc ctttcatgga gttgatgata tagaaaacag
     7081 aataatccgc acaccactcg atccggaaat tacctttagc gatgatccac tgcgaatgat
     7141 gcgagctatc cgatttgcat ctcaattgca attcacaatc gagcaaataa cttatgaagg
     7201 aattaaatcg caggttgaaa gaattcgaat tgtatcgttc gagcgcatca ttgacgaact
     7261 caataaaatt attctatcac cacaaccttc aagaggcttt gtgctattgc ttaatacagg
     7321 attattgaat attattttcc ctgagatgtg tgcgttgcaa ggggcagagt acaaagatgg
     7381 caaaggacat aaagataatt tttaccacac actggaagtg ctcgataata ttagcctcaa
     7441 caccgaagac ctttggctgc gttgggccgc catacttcat gatattgcaa agcctctcac
     7501 caagcgcttt gagcccggac agggatggac gtttcacggg catgaagaca aaggtgcccg
     7561 tatggtaccg cgtatttttg caaagctaaa attgcccctc aacgaaaaaa tgaaattcgt
     7621 gcaaaagctg gtggcgctgc atttgcggcc aatagtactt gcccaggaaa tagttactga
     7681 ttcggctgtg cgaagattgt tgtttgatgc cggtgaagaa attgacgcac ttatgttact
     7741 ctgcgaagcg gatgtaacaa caaaaaacga atacaagaaa aaaaaataca aagagaattt
     7801 tgagctggta aagcttaaat taatagaagt ggaagaaaag gatcgtatac gttattggga
     7861 gcctccaata agtggcgaag aaattatgaa aacctttaat ctgccaccgg ggcgcataat
     7921 aggcatcata aaaaaccata tacgcgaatc tattttggat ggagttattc caaacgagca
     7981 tgaaccggca tatctattaa tgttggctaa agctgctcaa cttggtttaa ctccggtttc
     8041 gattatcaaa taatattttg ttcagtttgc agttgatgta ttatctttgc atgcataatt
     8101 tcgttttcaa aatcaaaaaa tttcattgtc actaaattta cactatggca tcatataaat
     8161 tccgcataac atttgaagag tacgatgata taacgcgtga tatagaaata aaatcaacgc
     8221 agacatttct cgacttgcac aactgcctgc aagagtcaat aaaattcgac aattcgaagc
     8281 cggcatcatt ttttatgagc aacgacagat ggataaaagg caccgagatt tctttaaatc
     8341 caaaaacatt aaaagatggc accgatgtgg cattgatgaa aacagcggtg atgaataaat
     8401 acatttacga tcctcaccaa aaaatatatt acgagtttgg cgaatggtgc ttttttgttg
     8461 agcttatcaa aataaatact accgatgata aaaaccctta ccctcgttgt gtaaaggtaa
     8521 atggcgaagc gcccaaacaa tacaaagtgg tgaaagctat tcccggagta atggaactaa
     8581 acaatcttga actgcccgaa ggcgaacccg aacccgatga agatgatata gaagaaatag
     8641 aaacagccga tgccggaatt gtggaaggca tagatgaagg agaaacagaa atagggtttg
     8701 gaattgaaag cgatagtgaa gatgaaattc ctgaagaaga aatggatgag aatttcggca
     8761 acgatcacga agagatatag tgcctcgtga gcgataattt ttcttttata aatatacgat
     8821 gccaacattg atagttattt gtggtccaac ggcatcgggc aaaacacaag tggccataag
     8881 cattgcacag caattatccg caccgattat caattttgat tcgcgtcaat tttttcgcga
     8941 actaaatatt ggcacggcaa agcccacacc cgaacagctt gtacaggcaa aacatttttt
     9001 tatcgacagc cactctatta ccgatgaata taatgcagtt gcctttggta acgatgcaca
     9061 aaaattaatt gatgaacttt cacaccaaca ccaatatatt attctttgtg gtggttcggg
     9121 tttatatttg aatgcagtgt tgtttggtat ggatgcaatg cccgaaaaaa gcaaatcgat
     9181 acggcaaacc atcaataaaa attacgagca gtttggatta tcgtatttac aagaagaagt
     9241 aaaaaaagcc gatgcaaaat attatgaaac tgtagataca gaaaacccac acagactaat
     9301 acgcgcattg gaagtttata tgcaaacagg caaaccgttc tccgattttc gtaatggcca
     9361 aaataaaaat agtgcttatg actacaaact ttgtggcata actttacccc gcgaacaatt
     9421 gtacgaaaga ataaatcaac gtacagatat gatgattgca caaggacttg aaaacgaaac
     9481 acgacaagtg attgattacc gaaaacatca tgctttgcaa acagtaggtt acaaagaaat
     9541 gtttcaatac attgatggag aaataagttt gcagcaaacc attgacttga taaaacaaca
     9601 cacccgcaac tacgcaaagc ggcaaataac gtggttcaaa aaatatgaca atattaattg
     9661 gtacgcgccc aatgaagttg ataaaataat ggcagcactt taagcaaatt atgttttagt
     9721 catcacccta ttcaaactat aagcaaaatt ttctggctag attttatccc ctgattaaaa
     9781 aagatttttt ctcatttgga aaaaaaatat tgagcagcta tagcttgaaa aaagtgtgat
     9841 gaccgtgcaa ctattttatt ttttgtggat tactaaaaac cagataaagc cagcctaaaa
     9901 ttccaataca aagggtaaaa ataatttgac ctccatgtat gactattgcc agtgttttac
     9961 ccatagttgc acttatgttg tatactgcgg tgactttttc cactaaataa tgataggcgc
    10021 ccattccccc tccttgaatt ggaacgcttc ggccaatagt gcctatacta ataatgctga
    10081 ggcaaacgcc ccagccaaga tttcgggttt cgtcaaaggc aaaaaaccaa aaccaggtca
    10141 tgaaaaaata cccgacccaa atcaacaaag tatacaaata aaaaagccag gcatgtttta
    10201 atttattgat actcgtaagg ccattctttg cttcaataaa aatattttga acactttgtg
    10261 gcaggcgctt aaagcctctt tgcaataaaa acatgagcaa ccaacccaca gcaatgactg
    10321 caagcaaaat gattaatatc ttcaaatttc ctgtgtcaaa tttaactccg agactttgtt
    10381 taattggggt aataatattg ttgaggtaaa aaattctcat ttcagcattc tgcaccataa
    10441 atgtaaaaat aagcactata aataacgcaa gcacatcaat tatcctttcg gtaattacgg
    10501 tgccgagtac cgctacaaaa gggacttttc gtcttcgggc tatagcaatg caacgggtaa
    10561 tttctccaaa gcggggaaca ccaatgctta caaaatacga tgtggaaatt gcggcaaatg
    10621 tttctacaaa gtgtggacgg caattaatac tgcgcaacat catttgccag cgtaaagtgc
    10681 gcactacgta tacagctata gataccatga taaccacccc ataccacttt aaattggctt
    10741 tggcaatttc atttttaatt tcggtgagtg gagcgttttg cagaaacaaa taaatgagta
    10801 aaccacccac agtagcgtat aataaataaa gtactataga ttttaggttc ataaaaaaca
    10861 agaatatttt ttactcatca tcacttaaat ttcgccaatg tcttcgcccc aaataaaatc
    10921 gtcaagttct ctgcgctctt ttttcgtggg ccttccggca ccgggcatac gccatgccgc
    10981 agcagctttt cgcaaagcag catgttgcaa tagtgtttcc tcgggagtgg tttctttgca
    11041 atacttaaaa acatctttgg cacctactct actatcgagc aacatcaata cttcaaatgt
    11101 tcgaataagc gcaccatcct gaaatgtaac tatatcgccc ggcttaacat ttcgcgaagg
    11161 cttaataaca tcgcccttga ttttaatctt gcctttgtcg caatattgcg atgccatact
    11221 gcgcgttttg aaaatgcgaa cacaccacaa ccatttatct atacgcacag tggttatttg
    11281 tttttccatt tttttttgta gtgagtattt tagtattggg tattgagtag tttgtattga
    11341 gtactttgta tttattgctt ttgtattgag tatttagtat tgagtgcttt tgtatttttt
    11401 accttgtttt ttgttagctg ttttaaatca tttagagatt tgcttaggct tctattttat
    11461 tcgaaatcga aattatagaa aacaagtgaa atcataattc ctgtttttca ttttagaaat
    11521 ca
//
LOCUS       DAORQA010000044        11303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1561_length_11303_cov_15.529161, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000044 DAORQA010000000
VERSION     DAORQA010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1561_length_11303_cov_15.529161"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="PK736_04405"
     CDS             <1..1676
                     /locus_tag="PK736_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRC32663.1"
                     /translation="VLEKFTRGDSKNYLFLDVVISSNAKPGSMIISFGKNGKMVYTHS
                     YPLLQRGQDADLLKGFNASDVVCLIVPDRFANGDYTNDVVKGMRENKIDRAFDGGRHG
                     GDIRGIINHLDYISQLGYTAIWSTPLLENDMQKYSYHGYSITNHYKVDPRFGTLDDYK
                     ELAVKARQKGLKIIIDDVINHTGSNYWWMNDLPFKNWINYPDSLVITSHRRTVNQDKY
                     ASEYDKKIMTEGWFDTHMPDMNGANNFMANYLIQSSIWWVETLHAQGIRQDTYCYSDK
                     TFSKNWSCAIMNEYPEFNIVGEEWSLNPLITSYWQQGKINNDGYKSCLKAVMDFPLQS
                     ALISSLLDTSGSEYGRGITKLYEALANDFAYSNPNNILVMGDNHDMDRIYTQLHKDVA
                     LTKMAITYLLTVRGIPQLFYGSEILMDNSLHLSNHGIIRSDFPGGWNNDAADAFAAKG
                     LTSEQLDMQKWFAQILNWRKNNSVFADGELLHFAPFDNVYVYFRYNKEKMVMVVMNKN
                     TKPMNIDLKRFEEIMGAKTSARNIFTKAKLSIQNPIQVDAKSATVFELE"
     gene            1709..2632
                     /locus_tag="PK736_04410"
     CDS             1709..2632
                     /locus_tag="PK736_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRC32664.1"
                     /translation="MKYFITGLFVLFLNIVQAQLPNVCAGKLQRHENFISKFVTPRNV
                     DVWLPDGYSATKKYAVLYMQDGQMLYDSNLTWNKSAWGVDDIISKLNSENKIRDVIVV
                     AIWNGGLERHSEYCPQKPFETLPALQQEGIMNSIRRDGSSVFNGFKVKSDDYLKFVIT
                     ELKPFIDKTYSTHSDKENTFISGSSMGGLISIYAICEYPEIFGGAACLSTHWPVIFSI
                     ENNPMPFTIFDYLKNNLPSAKSHKIYFDFGTETLDKIYAPLQLEVDELMIAKGYTTKN
                     WMTKEFSGADHSEKAWHQRFDIPVLFLLGKE"
     gene            2865..4817
                     /locus_tag="PK736_04415"
     CDS             2865..4817
                     /locus_tag="PK736_04415"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC32665.1"
                     /translation="MTKKLFSELGLTDQVQSAIAAMGFEEPTPVQAQTIPAFLETESD
                     ILALAQTGTGKTAAFGLPLLCKLDFNSNHTQALVLCPTRELCMQITRDFKAYAKDMKG
                     VKIVAVYGGAGIFPQIDELRRGAQIVIATPGRLQDLMNRRKINIEQIDYVVLDEADEM
                     LNMGFRDDIEIILSTTPETKRVCLFSATMEKGVREIANKYLRKPIEITVGKKNTANVN
                     ITHEYAVVHAKDKFAALRRVLDFNADSFYGIVFCQTKIETQEISDKLVRDGYNADCLH
                     GDLSQQQREKVMGRFRQHSVKILMATDVAARGIDISGLTHVIHYHLPDDVENYTHRSG
                     RTARAGKTGISLTLLNVREFYKLRDIERISGISFARVLVPSPADVRDKKLLGIIDTIV
                     NTEIKSAIFDGIVVESMLPLMEMSTEELAQRVISLEMNRFSTDYLQGLDLNVYDQNNR
                     KAGGASAGGERSGGGYGNVTKLFINLGSRDGFTNDSMKQFVFENTGIEPKGLNFVNIK
                     GVYSFLEVTPDAIETVTKKLEGKFFNDRKIRIENHGQSSFAGTEKSQGGSRGGYGRSD
                     SRDSRSGGGSRSGGGYGGGRSSGGGYGGGAGSGGGNGKPRNAYGGGEGRSSSRGTGGS
                     PYEKRGGGSGYGSSGGDKAPRKRKTY"
     gene            5040..5666
                     /locus_tag="PK736_04420"
     CDS             5040..5666
                     /locus_tag="PK736_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRC32666.1"
                     /translation="MKKVYVNDKCLYLTDYQSRVASSPSLLSMAYSGHDTIQHVINTF
                     SKSKVYTAAEIFHPNFEELWDDFNEYFEMINAAGGLVLDENNRLLVIYRHNKWDLPKG
                     KVEDKELPEHAAVREVQEECGLAFCEIIKPLPYTYHTYALNNKQILKRTWWYLMRSNS
                     NEVLKPQAEEGITELKWITRSQLNEVYKNTFASIEALLKEEEESVFGA"
     gene            complement(5887..7443)
                     /locus_tag="PK736_04425"
     CDS             complement(5887..7443)
                     /locus_tag="PK736_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32667.1"
                     /translation="MKAIIILLFIFIHSLNIDAQIVFEKVYSLNAVGSGMAVRQTTDG
                     GFIFCGIGYSLLGGNDHEIFLVKTDSIGDTLWTRDFGTAFLYDRGHCVEQTIDGGYIL
                     TGMTMDSNYMDVETFLIKTDAYGDTIWTKRYKAPNDMYGNSVVQTPDGGFVIAGRAHG
                     IFALKTNSFGDLLWMKIFQGFFLDDCYCCVNTKDGGLMLTGCIYEVGSGDYLPFLLKL
                     DSLGNKLWAKTYNGPKWQLSHYVQQTQDGGFILTGSSRDSLSASYQFLLKTDSIGNIS
                     WAKQFDVSEPWKMGTAVQQTFDGGYIIATGSPIYHVGNGDGCLLKTDSVGQLLWSKTY
                     DVTLGEYFSDVRQTSDSGFIIIGFTSYSIVTGMCLIKTDQFGNSVCKQSGDAIINSYN
                     LSFTTSNLFTSASSYTPQELNSNFYVKSGSEFFDPCPFGYEEPFANNPLLIFPNPSSG
                     TFSLLLFTNSIDAKLEIFNLMGTKIFEEKIIGSVKNEITLTGMSSAIYLLKVYDGHKY
                     YHQKLMLECN"
     gene            complement(7649..8863)
                     /locus_tag="PK736_04430"
     CDS             complement(7649..8863)
                     /locus_tag="PK736_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRC32668.1"
                     /translation="MPQLSNKANLMPPSPIRKLVPFAEVAKKQGKKIFHLNIGQPDIA
                     TPDTMLDALRNHQLKVVEYSHSAGIESYRKKLTTYYDSFNIKLDYTEIMITTGGSEAI
                     EIAMMTCLNEGDELIVTEPFYANYNGFSCQAGISVKPVTAHIENGFALPPISEFEKII
                     TPRTKAIMICNPNNPTGYLYSRQELEELRKLVLKYDLFLFSDEVYREFCYDDKEYFSA
                     MHLEGVENNVVMMDSVSKRYSACGARIGALISRNKEVMASALKFAQARLSPPTFGQIA
                     AEAAIDTPPSYFEKVKAEYVHRRDVVVDALNKMDNVFCPKPNGAFYCIAQLPIDNADK
                     FCQWLLEEFDYNKQTVMLAPATGFYFNQELGRQQVRIAYVLKEDDLVAAMQCLEVALK
                     QYPGREASMVKK"
     gene            9176..10270
                     /locus_tag="PK736_04435"
     CDS             9176..10270
                     /locus_tag="PK736_04435"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRC32669.1"
                     /translation="MEVTSVVKSVVPQSNLFEVLESGMEHEQVVYCYDKETGLKAIIA
                     IHNTVLGPSLGGTRMWMYENESLALNDVLRLSRGMTYKASISGLNLGGGKAVIIGDAK
                     RDKTDAMMRKFGEFVNSLNGRYITAEDVGTSTADMVSVSQKTKHVTGLPELQGGGGDP
                     SPVTAYGVYMGMKASAKDMWGSDSLSGKCILVQGCGHVGQNLVKYVTEEGAKVFVSDI
                     HEDHLKKMVADYKAEVVDKDAVYDLEMDIYAPCALGATLNDITIPKLKCAVVAGAANN
                     QLHDEGKHGMMLLEKGIVYAPDFLINAGGLINVYSELQGYNREKALEATRHIYNVTSE
                     ILKKAKTEKISTVNAAKQIAEQRIRDKKNQ"
     gene            10316..11260
                     /locus_tag="PK736_04440"
     CDS             10316..11260
                     /locus_tag="PK736_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination protein NusB"
                     /protein_id="HRC32670.1"
                     /translation="MLNRRQLRIKVMQALYGHFQNENQDPNVAVNNLLTGNERSYDLY
                     LHLLQIFVELAQLEQTYKTDAPARMVGKSPFSFKFTLDDCLFVKWLKSDANYNELVRK
                     RTINWTLMHDDFATIYFNIRQTEEYKNFISTPDQPADVQIKFLRYIFENFFDKNDTFK
                     HNVEERNIYWAESFDLQAHFVYKTLDGFNPKNFMANRILPVYKEAEDDIDFVRMLFLK
                     TVKNNDLYTKMIAEKTQNWEVDRIAVMDVILLKMALCEILELPNIPVKVTMNEYLDIS
                     KVYSTPKSNAFINGIIDNLVIELREQNKIVKTGRGLVG"
BASE COUNT         3601 a         2052 c         2288 g         3362 t
ORIGIN      
        1 ttgtacttga aaaatttacc aggggcgaca gtaaaaatta tttattccta gatgtagtta
       61 tttcctcgaa tgcaaagcca ggttcaatga ttatttcttt tggaaaaaat ggcaagatgg
      121 tatacactca tagttatcct ctgcttcaaa gaggtcaaga tgctgatttg ctaaagggct
      181 ttaatgcaag cgatgttgtt tgccttattg ttcccgacag gtttgccaat ggcgactata
      241 caaatgatgt ggtaaagggt atgcgcgaaa ataaaataga tcgggcattt gatggtggcc
      301 ggcatggtgg tgatattcgt ggcattatca atcacctcga ttatatttcg caattgggtt
      361 ataccgctat ttggtctaca cctttgctcg aaaacgatat gcaaaaatat tcatatcacg
      421 gttactccat taccaatcac tacaaagttg atccacgttt tggaacgctt gacgattaca
      481 aagaattagc agtaaaggcg aggcagaagg gtttgaaaat tattattgat gatgtgataa
      541 atcacacagg tagcaactat tggtggatga atgatttgcc atttaaaaac tggattaatt
      601 atcccgactc acttgttata acaagccata gacgaacggt gaatcaggat aagtatgcct
      661 cggagtatga caaaaaaata atgacagaag gatggttcga tacacatatg cccgatatga
      721 atggcgctaa taattttatg gccaattatc ttattcaaag ttctatatgg tgggtcgaaa
      781 ctttgcatgc gcaaggaata cgtcaagata cttattgtta ttcggataaa actttttcga
      841 aaaactggag ctgtgccatc atgaatgaat atcccgaatt taatattgta ggcgaggagt
      901 ggagtttgaa tcctcttata acttcttatt ggcagcaggg caaaataaac aatgatggat
      961 ataaaagttg cctcaaagca gtgatggatt ttcctttgca atctgcattg atatcatcgt
     1021 tgcttgatac ttcgggcagc gagtacggac gaggcataac aaaattgtac gaggcattgg
     1081 caaacgattt tgcttattcc aatccaaaca atattctggt aatgggcgac aatcacgata
     1141 tggatagaat ttatactcaa ctccataaag atgttgcact aacaaaaatg gcgataacct
     1201 atttacttac cgtgcgcggt attcctcaat tattttatgg tagcgaaata ttgatggaca
     1261 atagtttgca tcttagtaac catggtatta tacgttccga ttttccgggt ggctggaata
     1321 acgatgctgc agatgccttt gccgcaaagg gtcttacatc agagcaattg gatatgcaaa
     1381 aatggtttgc acaaatttta aactggcgca aaaataattc ggtgtttgct gatggcgaac
     1441 ttttacattt tgcccctttc gataatgtgt acgtttactt ccgatacaac aaagaaaaaa
     1501 tggtaatggt ggtgatgaat aaaaatacaa agccaatgaa tattgattta aaacgcttcg
     1561 aagaaattat gggcgcaaaa acttcggcca gaaatatttt tacaaaggca aagttaagta
     1621 tccaaaaccc gattcaagtt gacgctaaat cggcaaccgt tttcgaatta gagtaaattt
     1681 gttttcatca catataaaaa atagtacaat gaaatatttc atcaccggct tattcgtttt
     1741 gtttttgaat atagtgcaag cacaattgcc aaatgtatgt gcagggaaat tgcaacgcca
     1801 cgaaaatttt atttccaaat ttgtaactcc gcgaaacgtt gatgtgtggt tgccagatgg
     1861 atattctgcc acaaaaaaat atgctgtgtt atatatgcaa gatgggcaaa tgttatacga
     1921 cagtaattta acatggaaca aaagtgcgtg gggtgttgat gatattattt cgaaattgaa
     1981 cagtgaaaat aaaattcgtg atgttatagt tgttgctatt tggaatggcg gattggaaag
     2041 acatagtgag tattgtccgc aaaaaccttt cgaaactttg ccggctttgc aacaggaggg
     2101 aataatgaat tcgataaggc gtgatggcag ttctgtattc aatggattta aggtaaagtc
     2161 cgatgattat ttgaaatttg ttattaccga gttaaaacct tttatagata aaacatattc
     2221 cacacacagc gataaagaaa atacttttat aagcggcagc agtatgggcg gattaatttc
     2281 tatttatgcc atttgcgaat accccgaaat atttggcggt gctgcttgct tatctactca
     2341 ttggcctgtt atttttagta tagaaaataa tcctatgccc tttacaatat ttgattatct
     2401 caaaaataat ttaccatcgg caaagtcgca caaaatttat ttcgattttg gcaccgaaac
     2461 tttggataaa atatatgcac ccttacaact tgaagtagat gaactgatga tagccaaagg
     2521 ttacacaaca aaaaattgga tgacaaaaga attttccggt gccgatcatt ctgaaaaagc
     2581 atggcaccaa agattcgata ttcctgtttt gtttttattg ggcaaggaat aagcaaaatc
     2641 accggcaggt aataagtttg aattcaaaga ataccctcag cagtttgttt gtaagtaaat
     2701 gcattaagga cgccagacat ctaaacagca gtttttattt tgtaccatac tattaccata
     2761 acctttactg tgtaagggtt gtaggctgcc ttcatgttgt atactaagta aatgcctaat
     2821 aaccgtaaaa aatgactacc tttgccactt cttaaaaata aacaatgaca aaaaaattat
     2881 tcagcgagtt aggattgaca gatcaggttc agtcggcaat cgctgctatg gggttcgaag
     2941 aacctacgcc tgtgcaggca caaacgattc cggcatttct cgaaactgaa tccgacatct
     3001 tggctttggc ccaaactgga acgggtaaaa ccgcagcctt tggactaccg ctgttatgca
     3061 aattagattt caacagcaac cacacacagg cattagtatt atgccccaca cgtgagttgt
     3121 gtatgcaaat tacacgcgac tttaaagctt atgctaagga tatgaaaggc gtgaagatag
     3181 ttgccgtgta tggcggagcg ggaatttttc ctcaaataga tgaactgcgc agaggagcac
     3241 aaattgtaat tgccaccccg ggccgtttgc aagatttgat gaacagacgc aaaataaaca
     3301 tcgagcagat tgattacgtt gtgcttgacg aagccgatga aatgctcaat atgggatttc
     3361 gtgacgatat cgaaattatt ttatcgacca cgcccgaaac caagcgtgta tgcctgttta
     3421 gcgcaactat ggaaaaaggg gtgcgcgaga ttgctaacaa atatttacgc aagccaatcg
     3481 aaataaccgt tggaaaaaag aacactgcca acgtaaatat tactcatgaa tatgcagtgg
     3541 tgcatgccaa agataaattt gccgctttaa gacgagtgtt ggattttaat gccgatagtt
     3601 tttatggcat tgtattttgc caaacaaaaa ttgaaacaca ggaaattagt gacaagctgg
     3661 tgcgcgatgg ttacaatgcc gattgcctgc atggcgacct tagtcagcaa cagcgcgaaa
     3721 aagtaatggg ccgctttcgt caacactctg tgaaaatatt aatggcaacc gatgtggctg
     3781 cacgaggtat cgatatttcg ggacttacgc atgttataca ttaccacttg cccgatgatg
     3841 ttgagaatta tactcaccgc agcggacgta cagcccgtgc cggtaaaaca ggtatatcac
     3901 ttaccttact taatgtgcgc gagttttata agttgcgcga tattgaacgt atatccggta
     3961 tcagttttgc ccgtgtgttg gttccatcgc ctgccgatgt gcgcgataaa aaactattag
     4021 gcataataga tactattgta aatacagaaa taaaatcggc cattttcgat ggcattgttg
     4081 tagagtcgat gctgccactt atggaaatga gtaccgagga attggcacaa cgtgttattt
     4141 ctttagagat gaatcgtttc tcgaccgatt acctgcaagg tttagatttg aatgtgtacg
     4201 atcaaaataa tcgtaaggcc ggtggtgctt ctgccggtgg cgagcgttcg ggtggtggat
     4261 atggaaatgt aacgaagttg tttatcaacc ttggctcgcg cgatggattt accaacgata
     4321 gcatgaagca gtttgttttt gaaaatacgg gcatcgaacc taagggcctt aattttgtaa
     4381 atatcaaagg ggtgtattca tttctcgaag taactcccga tgcaattgaa actgtaacca
     4441 aaaaattgga aggaaagttt tttaacgacc gcaaaataag aatcgaaaat cacggacaat
     4501 catcatttgc cggaacagaa aaaagtcaag gtggcagcag aggtggttat ggtcgaagcg
     4561 attcgcgtga cagccgttcg ggcggtggaa gccgcagtgg tggtggttat ggtggcggtc
     4621 gcagcagtgg cggtggatac ggtggcggtg caggcagtgg tggtggaaat ggaaaacccc
     4681 gcaatgcata cggaggtggc gaaggccgca gcagtagtcg cggaaccggt ggttcacctt
     4741 acgaaaaacg tggaggtggt tcaggatacg gaagcagcgg tggcgacaaa gcaccacgaa
     4801 aaagaaaaac atactagtgt tagctaacaa atagaaatag gaggtgagtt gtaaaaaatt
     4861 tacctccttt attttttcaa aatggatttt actttgattt ctttactcag gcaaatgcat
     4921 aactttgggt tttaaatatg ttcactgcat cgaaatcaga atttggtctt taatgtgatt
     4981 aaaacatttg aattggaaat ttaataaaac gaaaagcata atattctacg ctatttaaaa
     5041 tgaaaaaagt ttatgtaaat gataagtgcc tgtaccttac cgattatcaa tcgcgggtag
     5101 catcttctcc atcattgtta tctatggcat actccggcca cgatactata caacatgtta
     5161 tcaatacttt tagtaaatcg aaagtgtata cagctgccga aatatttcat cctaattttg
     5221 aagagttgtg ggatgatttt aatgaatact ttgaaatgat aaatgcggca ggtggtttgg
     5281 tgcttgacga aaacaaccgc ttgctggtaa tataccgtca caacaaatgg gacttgccaa
     5341 aaggaaaagt agaagataaa gaattgcccg aacatgcagc agtacgcgaa gtgcaggaag
     5401 aatgtggttt agctttttgc gaaataataa aaccattacc atatacgtac cacacctatg
     5461 cattgaacaa taaacaaata ctaaaacgta catggtggta ccttatgcgc agcaatagca
     5521 acgaagtact aaaaccccag gccgaagaag gcataaccga attgaaatgg ataacccgca
     5581 gccaattgaa cgaagtgtac aaaaacacgt tcgcatcaat agaggcattg ttgaaagagg
     5641 aggaagaaag tgtttttggg gcctgaaaat gatagtctat ttttatttga ttttagagac
     5701 aatttttttc attattctta aaaaaaactt tgttgcaaat agattttctt ttttagttct
     5761 tttaaaattg cttcaacatt agtttcattt taatattcac gcaagagaat ttttatttca
     5821 atgcatgcat tatttttttt gattttgtaa ttatattggt ctggttatag agttaaatta
     5881 ttgttctcaa ttgcattcca gcataagctt ttgatgatag tatttgtggc catcgtatac
     5941 ttttagcaaa tatattgctg aagacatacc tgttaatgta atttcatttt taactgaccc
     6001 gataattttt tcttcaaaaa tcttcgtgcc catcaggttg aatatttcaa gtttggcatc
     6061 aattgaattt gtaaaaagca atagagagaa agtgcctgaa gacggatttg gaaaaatcaa
     6121 taatgggttg ttcgcaaagg gctcttcgta accgaacggg cagggatcaa aaaattcgct
     6181 cccgctcttc acataaaaat tcgaatttag ttcttgtgga gtataagatg atgctgatgt
     6241 aaataagttg ctggttgtga aactcaaatt atatgagttt attattgcat caccactttg
     6301 cttacagaca ctgttgccga attgatcagt tttaattaaa cacatacctg tgacaattga
     6361 atatgaagta aagcctataa taatgaagcc gctatcggaa gtttgtctaa catctgaaaa
     6421 atattctcct aaagtaacat catatgtttt agaccacaat agttgtccaa cagaatcagt
     6481 tttcaataaa catccatcac catttcctac atgataaatg ggactgcctg tggctattat
     6541 gtaaccaccg tcaaaagttt gctgaacagc agttcccatt ttccatggtt cactcacatc
     6601 aaattgtttg gcccaactaa tattcccaat agaatctgtc ttgagtaaaa attgatagct
     6661 tgcacttaaa gaatctctgg aactaccggt taaaataaat cctccatcct gcgtttgttg
     6721 cacatagtga ctcaactgcc atttcgggcc attatatgtt tttgcccata gtttgtttcc
     6781 tagagaatcc aatttcaata aaaatggtaa ataatctcct gaacccactt catatatgca
     6841 ccctgtaagc ataaggccac catcttttgt gttcacacaa cagtagcaat catctaaaaa
     6901 gaagccttga aatatcttca tccacagcaa atcgccaaag ctatttgttt tcaaggcaaa
     6961 aataccatga gctcggccgg caatgacgaa acctccatct ggcgtttgta ccactgaatt
     7021 gccatacatg tcatttggag ccttatatct tttggtccaa attgtatcac catatgcatc
     7081 tgtttttatt aaaaatgttt ccacgtccat atagttcgaa tccattgtca ttcctgttaa
     7141 tatatatcca ccatcaatgg tttgttctac acaatgacct ctatcataaa gaaaagccgt
     7201 tccaaaatca cgtgtccata aggtatctcc aattgaatca gtttttacta aaaatatttc
     7261 atgatcattt ccacctaaaa gtgaatagcc aattccgcaa aaaataaaac ctccatctgt
     7321 tgtttgtcgg acagccatgc cagaaccaac cgcatttagg ctatatactt tttcaaaaac
     7381 gatttgagca tcgatattta agctatgaat aaaaataaat aaaagaatga ttatcgcttt
     7441 catgttggta tgatttgaaa tagtaaaaag taaattttag aaaatgttct ctgttggtgt
     7501 ctttgctgcc taattttttt cactgataat ctaattaaaa atcatagcct atttgattaa
     7561 caaaattact tcacgaatac caaagttact ccccaatcaa aaactcttga actattgaac
     7621 ggttcaactt ttaaacctcc taaaaaaatc acttcttcac catcgaagct tcgcgtcccg
     7681 gatattgttt cagcgcaact tctaaacatt gcattgcggc caccaagtca tcttctttca
     7741 atacataagc tatgcgcact tgttggcggc caagttcctg gttgaaataa aatcctgtgg
     7801 caggtgcaag cattacagtt tgtttgttgt aatcaaattc ttctaaaagc cattggcaaa
     7861 atttatcggc attatcaatt ggtaattgtg ctatgcaata aaaagcacca tttggttttg
     7921 ggcaaaaaac gttgtccatt ttatttaatg catcaacaac tacatctctg cggtgcacat
     7981 attcggcttt cactttttcg aaatatgatg gtggagtatc tattgcagcc tcggccgcaa
     8041 tttgtccaaa tgtgggaggg cttaaacgag cctgtgcaaa cttcaatgcc gatgccatta
     8101 cctctttgtt gcgcgatatc aatgcaccaa tccgcgcccc acatgcacta tatcgcttgc
     8161 ttaccgaatc catcatcacc acattgtttt ctacaccttc gagatgcatg gccgaaaaat
     8221 attctttatc gtcataacaa aactcgcggt aaacttcatc cgaaaataaa aacaaatcgt
     8281 atttcaaaac cagtttacgt aactcttcca gttcctggcg cgagtataaa tagcccgtag
     8341 gattattggg attacaaatc attattgcct tggtgcgtgg ggtaattatt ttttcgaact
     8401 cgctgatggg aggcagtgca aatccgttct caatatgtgc agtgacaggt tttaccgata
     8461 tgcccgcctg gcacgaaaat ccgttgtagt ttgcataaaa tggttcggtc acaatgagtt
     8521 catcgccttc gttgaggcat gtcatcatag caatttcaat tgcttcgctg ccaccggttg
     8581 ttatcattat ttcggtgtag tcaagtttaa tattaaagct atcgtagtat gtagtaagtt
     8641 ttttgcggta gctttcaata ccggcactat ggctgtattc taccaccttc aattgatggt
     8701 tgcgcagggc atcgagcatg gtatcgggtg tagcaatgtc gggctgacct atattgagat
     8761 gaaatatttt tttgccttgt ttttttgcca cttcggcaaa gggtacaagt ttacgtatgg
     8821 gcgaaggagg catcaagttg gccttgttcg ataattgagg catagctata tattgatttt
     8881 ttgtgtggtc aaatatcaca attattaatt ataagattgt agcataatga gaaaaattat
     8941 ttgaacgaat aattagattt tataactccg tttttattac tcaatcaaaa aatgacttca
     9001 gcaggtcgac tcatgcaaaa taaattttat aaacaatttt tatgcataaa gaccattaca
     9061 acaacagaaa cttcgctatg aaaaaaaagc actattttaa aaaatcaaaa aattaattat
     9121 cttttatttg cggtgatgaa aaaaatttac ctttggcaca attaacattt agtttatgga
     9181 agtaacaagc gttgtaaaat cagtagttcc tcaaagcaat ttattcgagg ttttagaaag
     9241 tggaatggag cacgagcagg ttgtatactg ctacgataag gaaacaggat taaaagccat
     9301 tatagctatt cacaataccg tattggggcc atcgcttggt ggcacacgca tgtggatgta
     9361 cgaaaacgaa tcgcttgcat tgaacgatgt actgcgcctt tcgcgaggta tgacctacaa
     9421 ggcttctatt agcggcctaa accttggcgg tggcaaggca gtaattatag gcgatgcaaa
     9481 acgcgataaa accgatgcca tgatgcggaa gtttggcgag tttgtaaaca gcctcaatgg
     9541 acgatacatt actgccgaag atgttggcac aagcactgcc gacatggtta gtgtttcgca
     9601 aaaaacaaag cacgttacgg gcttacccga attgcagggt ggtggtggcg atccttcacc
     9661 cgttactgca tatggcgtat atatgggtat gaaagcctcg gctaaggata tgtggggaag
     9721 cgatagtttg agcggtaagt gcatattggt gcaaggctgt ggtcatgttg gccaaaatct
     9781 ggtaaagtat gtgaccgaag aaggcgcaaa agtttttgtt agtgatatac acgaagatca
     9841 tttaaaaaag atggtggcag attataaagc tgaggtggtt gataaggatg ccgtttatga
     9901 tttagaaatg gacatatatg caccctgtgc ccttggtgct actttaaacg atattacaat
     9961 accaaaattg aaatgtgccg tagtagcagg tgctgccaat aatcagctgc acgatgaagg
    10021 caaacatggc atgatgctgc tcgaaaaagg aattgtttat gcacccgatt ttttgataaa
    10081 tgccggtgga cttataaatg tgtatagcga gttgcaaggc tataaccgcg aaaaagcttt
    10141 ggaagccacc cgccatattt acaatgttac ttccgaaatt ttgaaaaaag ccaagaccga
    10201 aaaaatttct accgtaaatg ctgcaaagca aattgctgag caacgcattc gcgataaaaa
    10261 aaatcaataa tcattcagtt acaataacag ttctttcttt taggtaaatt caattatgct
    10321 gaacagacgt cagttacgca tcaaggtgat gcaagcttta tatggtcatt ttcaaaacga
    10381 aaatcaagac ccaaatgtgg ctgtaaataa tctgctcaca ggcaacgaac gctcttacga
    10441 tttgtatctc cacttgttac aaatttttgt agaactggca caattagaac aaacctataa
    10501 gacagatgca cctgcccgta tggtgggcaa aagccccttt agttttaaat ttactttaga
    10561 tgattgcctt tttgtaaaat ggctcaagag cgatgcaaac tacaatgaac tggtgcgcaa
    10621 gcgaaccata aactggacat tgatgcacga tgatttcgct accatttatt tcaacatccg
    10681 ccaaaccgaa gaatacaaaa attttatttc tacgcccgat caacctgccg atgtgcaaat
    10741 aaaattccta cgttatattt tcgaaaactt ttttgataag aacgatacct tcaagcataa
    10801 tgtggaagag cgcaatattt attgggccga aagtttcgat cttcaagcgc attttgtgta
    10861 taaaacactc gatggtttca acccgaaaaa ctttatggca aatcgaattt tgcctgtgta
    10921 taaagaagcc gaagacgata tagattttgt gcgcatgctg tttttaaaga cagtaaaaaa
    10981 taacgacctc tatacaaaaa tgattgccga aaaaacccaa aattgggaag tggatcgcat
    11041 agcagtgatg gatgtaatat tacttaaaat ggcactatgc gaaattcttg aattgcccaa
    11101 tattcctgta aaagtaacca tgaacgaata cctcgatatt tcgaaagttt atagcacacc
    11161 aaaaagtaac gcctttataa atggtattat tgataacctc gttattgaac ttcgcgaaca
    11221 aaacaaaatt gtaaaaaccg gacgtgggct ggtggggtag tttttggttc ttagttttta
    11281 gtttatagtt cttagtttat agt
//
LOCUS       DAORQA010000045        11137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1584_length_11137_cov_35.378903, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000045 DAORQA010000000
VERSION     DAORQA010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11137
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1584_length_11137_cov_35.378903"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..874)
                     /locus_tag="PK736_04445"
     CDS             complement(347..874)
                     /locus_tag="PK736_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32671.1"
                     /translation="MPTKKSKSDTLNLSNSGIIMGSRVDTFTLTPLNFYIKDKGDLAA
                     MHPLFWDRMFIDFDSRSKLYIHIEKNLKGNKVQLSLLLNSFYPGNNPPKPIGLIDVNF
                     NDFDFPKKNTGKIPVPLQSTPGFTEYYVQSSDVCNRDGTAVELTFTDFVDLMLVQVKV
                     EIFTPRALAFLPRNK"
     gene            complement(1152..3386)
                     /locus_tag="PK736_04450"
     CDS             complement(1152..3386)
                     /locus_tag="PK736_04450"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRC32672.1"
                     /translation="MRKKLLKLTCGFIATIWCINVNAQSRIKKNYISDDGFQNNQSVL
                     RSFEGGMEVLQKKGGQTFLPSSLSGYYFEGFESAFPPAGWQIVDVLDNVNTWTSSTLA
                     DYPDAFEGAQAAYCHYAYPVGPGEDWFISPKFTVAAGDSLMFQFKWESIGWQYDSTLI
                     LISTTDSALSSFSTLVDSYVDTSTVQILNVPDWYYKSYSLNAFTGQDIYVAFVNKNTN
                     GDGVFIDNVELGTRPVAEVAVKSIDMDRYYPTGTSNPKASVKNYGSITQTFDVTMTIN
                     GGYTSTKSVTVSPQATTQISFDPWYATVGNYTINVQTLLTGDVNSANDTISKSINVLE
                     SFVNYGWSTHDPLVAPVMGSAAVSVCTNTNSSCFLMGGIATGNILVDANKYDLSFGTW
                     AGISPMTVKSCFAGAAHANGKIFVLAGTAFTNGNPDGTTKIYDYVTDTWSTGTPMPIP
                     VANCAFGTYKDSLIFIIGGNIGNAVASNNVQIYNTYTDVWSVGTAKPGTPVYAIRAGI
                     AGNKIVVTGGYDPAAAISIGTTYVGAIDSVDPTEITWTQMADHPAGKISRTGPGVSLD
                     KNSSLVVFAGGTNTNTTATTTAKTFAYDVNSNAWKIGPDKPTPLNLFYMTPVVQNDSL
                     YLAALGGNDGVNESDKNEWLNLGFYQFPIGIEENNFVAEVNLFPNPASDATTLSLNLK
                     NASQVKITISDVIGNTVMIVCDKKMDAGKNSISISSKNLESGMYFCAINVDGKTITKK
                     IIKN"
     gene            complement(3552..3821)
                     /locus_tag="PK736_04455"
     CDS             complement(3552..3821)
                     /locus_tag="PK736_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32673.1"
                     /translation="MKKATLKIICTLSAIIFCISVNAQCNIQILSIDDTEGIIAGMNY
                     APKATVKNINSADETFIITMKISNGYESRRQFSLSKKCGNYFCIR"
     gene            complement(4217..6187)
                     /locus_tag="PK736_04460"
     CDS             complement(4217..6187)
                     /locus_tag="PK736_04460"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRC32674.1"
                     /translation="MIASIKFSINKKCIKFLYITLVLIANTRVGISQPKESTHLNEGI
                     TEMPKSFSIKSSIDTTFDLMLITEIYDDTFSLFKKYDAIQFQKFKSLNGFVLSKYHKD
                     FSTMTQWLKFKIENKTDATINGYYYCGRHNLISIAESDSNKIQFGGMAHYEPQKSKLF
                     PQYYFPISLPANTVKQYFVSITNYGILPSPIESKLILSQVHPTTSNIMNHYYFGWLIF
                     TCLVCGGILILGIFNFVQFFSNWKREYLYYGFYALMMFFSIERASEWNFELRIISQAL
                     PNYFFSRAVMFNVLSGIFYLLFTRHFLDIKNIDLSIYKTIKFILTVLVIGFIFLCICI
                     FANITEHWFGYVSAFLSFAPIAGSTFVAIQIFLKMRKSPLAKYYLLGYAFLFLGAGTN
                     IYINNLGRQFIQRQHPQIIYLEVGVLFEIILFAVGLGYKSRLLEKQKINAEVKNVKLQ
                     LDNELNILQLRNRLSRDLHDDIGSTLSSINILSRTAQNNLQQTDSEKTSDTLKKINER
                     SQRLLTNMSDIIWNIKPNNDTFEELMSRMREYGTVILEAKKIQYEINFPTEKIDYKLT
                     MEAKRNLYLIFKEAVNNLAKYSGATQAKLILNFDNAHIHMKIEDNGAGFNINELRHRG
                     GLTNMQHRAEEMKGKLKIESVIGQGTVVELIL"
     gene            6371..7714
                     /locus_tag="PK736_04465"
     CDS             6371..7714
                     /locus_tag="PK736_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRC32675.1"
                     /translation="MSSLTRSLSLAQASAINVIDMVGIGPFVALSFVMSAMGGAPCML
                     AWLLGALLSMLDGFTWAQLGSALPKAGGSYIFLQKLYGENRWGRLFSFLYIWQTSIQA
                     PLVIASGAIGFATYFNYVYPVDDFQRKCISGALVVLIVALLYRKIGSIGNISLMLSVV
                     VMGIMLWLIASGWIYDSPLSVSWAQVWNVTFDKSFFTQLNEASLKTIYCFLGYYNVCH
                     LGGEIKNPQKNIPRSIFISIAIITCLYLAMQWSVLRVISVNEAASHEYVVSMFFEKIY
                     NRQVALFATGGILIVAFSSLFAVVLGYSRVPYAAAVDGNYFKIFAKLHPRLNFPYMSL
                     LILGSIAFVFSLLFKMREIISAIIVVRILIQFVSQALGIIIFNKNIVQQSPTFKMPLF
                     PLPAILSIIIWLYIFGWSQWVYIAGAFVIIITGVILFLILSYQQMKWPFLKNEKR"
     gene            7701..9722
                     /locus_tag="PK736_04470"
     CDS             7701..9722
                     /locus_tag="PK736_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC32676.1"
                     /translation="MKKDNFKKNTHEKNITKGFGVPKGEPDKKTAFDIIGILIIVLLG
                     IIIYSNTYHCTFHLDDAHTIVDNSAIKDLFNIKAIWNNNHNRFIPYLSFAINYHFGKL
                     DVTGYHLVNIIIHLMNACVVFMLTKLLFSTVVLKQSVIAQHKNIFALVTALLFVSHPL
                     ATQSVTYIVQRMASLVTLFYLLTVTLYVKGRITENNNFSKFAWFSGSIIMAMLAMLSK
                     ENAITLPLAIIMIEIFFLQSKKTFLILKDYRIVVLSILFISIILFVAFRFSSGIFNPL
                     PPDAITNFVEITPANFLFTQFSVLLKYLQLLLFPINQNIDYDITLSTSFFEIKTLISF
                     ILLAAILVLGVLLFNKSRIISFGIFWFFVTIAVESSIIPISDLIFEHRTYLPSYGFML
                     ILTTAIFTLLWPKNKNFALAIFGLIIISNAVLTYQRNKVWQDEITLWTDAIKKSPNKA
                     RPYVIRGNAYEDLNKMDDAILDYNKAIELQPNYTLAFTNRGYAHKHKGNWQKAIQDYN
                     KSISLNPLNALAYTNRGVIYGNLKQWDKAIEDYTAAIKVDNRFVLAYSNRAMAYATLK
                     QWDNAIVDCSQAIAIDPYKSGAYVNRGVAYQALGKIDIAIADFTKAIELSPRNVNYYI
                     NRGRIYLEQREWNKAINDFTVALQLEPKNAKALKAREYAYKQLNIKTNF"
     gene            9866..10747
                     /locus_tag="PK736_04475"
     CDS             9866..10747
                     /locus_tag="PK736_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC32677.1"
                     /translation="MSDNNNKNREYANLPLHVYAAGKQPGIVKTLLTIAKEFPAAHSL
                     GYRFAKRNIKARYTQSILGIVWALLPNISIAAVWIILHNNKIVTVSHTGGNYALFVII
                     GIMLWAVFTKAVLTPLQIIQSNRGVLVKINFPREALVILSFYETLFNAVITLIVIVAS
                     MIYFQVPVSFSSLLFFPSLLLLNIMGLCIGTLLVPVSVLNNDVNFLLPTFLQFAMYLT
                     PVVYAQTTSNGILQILSLNPVSPILTIARNFLLGIDSPISWIQLLIVTLVTLVLFIAG
                     IIIQRFAMGILIERMGS"
     gene            10751..>11137
                     /locus_tag="PK736_04480"
     CDS             10751..>11137
                     /locus_tag="PK736_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC32678.1"
                     /translation="MSENTEILVDVKNVSKKFSLNLRTSMMYGLKDLGKTMLGIPYDS
                     SQLREKEFWAVKDISFQLRRGECLGLIGRNGAGKSTLLKLLNGLIRPDEGSITMRGKV
                     GALIELGAGFNPLLSGRENVYNNGQLL"
BASE COUNT         3433 a         1948 c         1998 g         3758 t
ORIGIN      
        1 ttgtagttga ttttatttct aacaagcttg atgcagcagg agcattggta ccaatacctg
       61 cacttccatt tgcaggaaat gtgttttgag cgtttcccgt tataacacaa atcagccagc
      121 aaattgagat aaggattttt ttcataatga tttttattaa tgaattaatt gataaatatt
      181 tcttcaaatg aataatattt atttcgattt taaaatggaa ctgtatcgta catcaaaaaa
      241 tttcctactg cccaaatgcg tggaactgaa atcaacattt caacaaatgg ggttaatcgt
      301 tcctccaaaa aatggagaaa cgattagccc aagattaatt ttatttctat ttatttcgag
      361 gcaagaaggc gagagcccga ggtgtaaaaa tttctacttt cacttgcact aacataagat
      421 caacaaaatc agtaaaggtt aattccacag cagtcccatc tctgttacac acatccgaac
      481 tttgtacata gtattcggtg aaacccggag tcgattgtaa tggcacagga attttacctg
      541 tgtttttttt gggaaagtca aagtcgttaa aattcacatc tattaatcca attggttttg
      601 gtgggttatt accaggataa aatgagttaa gcaacagact taattgcact ttgttgcctt
      661 tcaaattttt ttcaatgtga atgtaaagct tagatcggct gtcaaagtca ataaacattc
      721 tatcccaaaa cagcggatgc atagctgcta agtccccttt atcttttatg taaaagttaa
      781 gtggcgtgag cgtaaaagta tcaactctac ttcccataat aataccgctg tttgaaagat
      841 taagcgtgtc tgattttgat ttttttgttg gcattttgtt tatttttaag attaatattt
      901 tgttttatcg gcagcaaaca tacagtgaac aaatgaataa agaaccccca taaacatgtg
      961 gggggaatac ccttgtttga gttggggagg ttggggaggt tttgaaagtt tggatacttc
     1021 ggtcgggatg ccgaccagag tataaaattt ttatttcgtt ttttatttat aaaaacatgt
     1081 tgccaatata ggttgaagaa aaagagtctc acaaataatt caatcacaat attggcaaca
     1141 cgttctcagg tttagttttt aattattttt ttagtgattg tttttccgtc cacatttata
     1201 gcgcagaagt acattccgct ttctaaattt tttgatgaaa tagaaatact gttttttcct
     1261 gcgtccattt ttttatcgca aacaatcatt acggtatttc caatcacatc actaattgta
     1321 attttaactt gcgatgcatt ttttaaattc agtgatagtg tggttgcatc gcttgcagga
     1381 tttggaaata agtttacttc agccacaaaa ttattttctt caatgccgat gggaaattgg
     1441 tagaagccca ggttgagcca ttcgttttta tcactttcat ttactccatc atttcctcct
     1501 aaggcagcga gatataaact atcgttttga acaacaggag tcatataaaa taaattaagt
     1561 ggtgttggtt tatctggccc tattttccat gcgttagaat ttacatcata cgcaaaagtt
     1621 ttcgctgtag ttgttgccgt agtatttgta tttgttccac ctgcaaaaac aacaaggcta
     1681 gaatttttat caagagatac tcccgggccc gtccttgata ttttgcccgc aggatgatct
     1741 gccatttgtg tccatgtaat ttcagttggg tcaaccgaat caattgctcc aacataagta
     1801 gtgccaattg atattgccgc tgcagggtca tagcctccgg taacaacaat tttatttcct
     1861 gcaattccgg cacgaatagc ataaaccgga gtgccgggtt tagctgttcc tacactccat
     1921 acatcagtat aggtgttata aatttgaacg ttgttgctgg ctactgcatt accaatattc
     1981 ccaccaataa taaaaataag cgaatctttg taagtgccaa aagcgcaatt tgccactgga
     2041 attggcattg gggttcctgt tgaccaagta tcagtaacat agtcatatat ttttgttgtt
     2101 ccgtcagggt ttccgtttgt aaatgctgtg cctgccaaaa caaatatttt accatttgca
     2161 tgtgctgcgc ctgcaaaaca agatttaaca gtcataggac tgatacctgc ccaagttcca
     2221 aaactcaaat cgtatttatt agcatccaca agaatatttc ctgtggcaat cccccccatt
     2281 aaaaaacaac ttgagtttgt attagtacaa acagaaaccg cagcacttcc cattacagga
     2341 gctactaatg ggtcatgtgt actccatcca taatttacaa aagattctaa aacatttatt
     2401 gattttgaaa tcgtatcgtt tgcagaattt acatcacctg ttaacaatgt ttgcacattg
     2461 attgtgtaat ttccaacagt ggcataccat gggtcgaaac ttatttgtgt tgttgcttgt
     2521 ggagaaacgg taacagattt tgtggatgta taacctccgt ttatagtcat agttacatca
     2581 aatgtttgag taatgctgcc ataatttttt acacttgctt ttggattaga agttcctgtt
     2641 gggtaataac gatccatatc aatactcttc actgctactt cggcaacggg gcgtgtgccc
     2701 agttctacat tgtcaataaa aacaccatca ccattggtgt ttttatttac aaaagcaaca
     2761 taaatatcct gaccggtaaa tgcattgaga gaatagcttt tgtaatacca atcgggcaca
     2821 tttagaattt gaacagtgct ggtgtcaaca taagagtcaa ctaaagtgct aaagcttgaa
     2881 agtgcagaat cggttgtaga aattaaaatc aaagtagagt catattgcca gcctatggat
     2941 tcccacttaa attgaaacat aagcgaatca ccagcagcta ctgtaaattt tggtgaaata
     3001 aaccagtctt caccgggacc aaccggataa gcatagtggc aatatgcagc ttgcgcccct
     3061 tcgaacgcat cagggtaatc agctaatgtg gaagaagtcc aggtattaac attatccaac
     3121 acatccacaa tttgccaacc cgcaggagga aatgcacttt caaatccttc gaaataatat
     3181 ccgcttagtg atgaaggcaa aaatgtttgt cctccctttt tttgaagcac ctccatacct
     3241 ccttcaaagg aacgcaacac cgattgattg ttttgaaagc catcatcaga aatataattt
     3301 ttcttgatgc ggctttgtgc gtttacattt atgcaccaga ttgttgcaat aaatccgcaa
     3361 gttaatttta gaagtttttt tctcattttg gtttttttta tttgattaaa atattttatt
     3421 ggtcttgttt gtaaagtagc gaatctgcaa ttatggcaac ttcattagaa agtgtgttaa
     3481 catcggtatc atcatcgaat tgagattgta tggtaacgga aaaaatgtaa ttacctttaa
     3541 taaaattcac ctcatcgaat acaaaagtaa tttccgcatt tttttgaaag gctaaactgc
     3601 cttctacttt cgtaaccatt cgaaattttc atggtaataa taaaagtttc gtcagccgaa
     3661 tttatatttt taactgtcgc ctttggagca taattcattc cggctataat tccttcggta
     3721 tcgtcaatag aaagtatttg aatattgcat tgcgcattta cgcttatgca aaaaattatt
     3781 gcacttaatg tgcagataat ttttagtgtt gcttttttca ttttgatatt ttttaaatga
     3841 ttaataattt taatggtgta aaaatacatt ggccatcagc aaaataaaac cccataaaca
     3901 tgtgggggga atacccttgc ttttggaaat tagaaattaa gaattgggga tgagaaattg
     3961 gggattgggg aaattaggaa ttggtaattt gccatgagaa attggggatt ggaaaataag
     4021 aattggggat tggggatgag aaattaagaa ttggggatga gaaacttcgc aagactaaac
     4081 aaactagcct gatggaacac tgctcacgct gatacaacgg atgagtgcgg attttttaaa
     4141 tctttttaaa tcagtttcat ctgtgttcta ctttttttgc taagaaaaat aaattaatga
     4201 atccacattt gtgcaatcat aaaatcaatt caacaacagt cccctgccct atcacgcttt
     4261 caattttcaa tttgcccttc atttcttcgg cccggtgttg catattggtc aatcctccgc
     4321 gatgtcttaa ttcattgatg ttgaagcctg caccattgtc ttctattttc atgtggatat
     4381 gtgcattatc gaaatttaaa attaattttg cttgtgttgc gcccgaatat ttggcaagat
     4441 tgtttactgc ttctttaaat attaaataca aatttcgttt ggcttccatg gtcagtttgt
     4501 aatcaatttt ttcggtgggg aaatttattt cgtattgtat tttttttgct tcgagtataa
     4561 cagttccata ttcgcgcatg cgactcatta gctcttcaaa agtatcattg ttaggtttta
     4621 tattccaaat aatatcgctc atgttagtaa gcaagcgttg gctgcgttcg tttatttttt
     4681 tgagggtgtc gcttgttttt tcactatcgg tttgttgcaa attattttgt gctgtgcggc
     4741 tgagaatatt tatgctgctg agtgtgctgc caatgtcatc gtgtaaatcg cggcttaggc
     4801 ggttgcgcaa ttgtaaaata ttcaattcat tatcaagttg gagttttaca tttttaactt
     4861 cggcatttat tttctgcttt tccaacaggc gcgatttgta acccaatccg actgcaaaca
     4921 aaataatttc gaacaataca ccaacctcta aataaataat ttgagggtgt tggcgttgaa
     4981 taaattgacg gcctaaatta ttgatataaa tatttgtgcc ggctccaaga aataaaaatg
     5041 catatcccaa taaataatat tttgctaatg gtgattttcg catttttaaa aatatttgaa
     5101 ttgcaacaaa tgtagagccg gcaatgggcg caaaacttaa aaatgcactc acatatccaa
     5161 accaatgttc ggttatgttt gcaaaaatac aaatacataa aaagataaaa ccaataacca
     5221 aaactgtcag gatgaattta atggttttat aaattgataa atcaatattt ttaatatcaa
     5281 gaaaatggcg ggtaaaaagc aaataaaaaa tacctgacaa aacattaaac attaccgcgc
     5341 gtgaaaagaa ataatttggc aatgcctgag aaataattct cagttcgaag ttccactccg
     5401 atgcgcgctc aatagaaaaa aacatcatca atgcgtaaaa tccataatac aaatattcgc
     5461 gtttccagtt cgaaaagaat tgtacaaaat taaaaatacc aagaattaaa attccaccac
     5521 atacaaggca agtaaaaatt aaccacccaa aataataatg gttcataata ttgctggtgg
     5581 taggatgtac ctgagataaa atcaatttcg attctatagg gcttggcagt attccataat
     5641 tagtaatcga aacaaaatac tgttttacag tattcgcagg taaagaaatt ggaaaataat
     5701 attgaggaaa taatttgctt ttttgaggtt cgtaatgagc cataccccca aactgaattt
     5761 tattagaatc actttcggca atgcttatca aattatggcg cccgcaatag taataaccat
     5821 taattgtggc atccgtttta ttttcaattt taaacttcaa ccactgggtc atggtggaaa
     5881 aatctttgtg gtatttcgat aaaacaaaac cattcaatga tttgaacttt tgaaattgaa
     5941 tggcatcgta cttcttgaat aaagaaaaag tatcatcata tatttctgtg attaacatca
     6001 aatcgaaggt ggtatcaata gaacttttta tggagaatga ctttggcatt tccgttattc
     6061 cttcatttaa atgcgtgctt tcttttggtt gtgaaattcc aactctggta ttggcaatca
     6121 acacaagtgt gatgtataaa aatttaatac actttttatt tattgaaaat tttattgatg
     6181 caatcacttt tataaaatgg tagagttttt aaaagtatat ttttttaatg cacaaaaaaa
     6241 ttatttcgaa aattatgttt gttatacaag aagcacgaaa tgtttatccg aacttttaaa
     6301 acaagttaaa atgaaatggc gcttccagtt ttaaactaat ttctacattg cacgcaaagc
     6361 caattaatat atgtcgtcac ttacccgcag tttaagtctt gcacaagcca gcgccatcaa
     6421 tgttatcgac atggtgggca ttggcccgtt tgtggcgtta tcatttgtga tgagtgccat
     6481 gggtggtgcg ccatgtatgc tggcgtggtt gctgggtgca ttgcttagta tgctcgatgg
     6541 ttttacatgg gcacagctgg gttcggcatt gccaaaggcg ggaggtagct atatttttct
     6601 gcaaaagttg tatggcgaaa atcgttgggg tcgcctgttt tcattcttat acatatggca
     6661 aacttctata caggcaccat tggtaatagc atcgggtgca attggttttg ctacttactt
     6721 caactacgta tatccagtcg atgattttca acgcaaatgt atttcgggcg cattggttgt
     6781 tttaattgtg gctttgctct atcgtaaaat tggttctatt ggcaatatat cgttaatgtt
     6841 atcggtggtg gttatgggca tcatgttgtg gctaatagca agcgggtgga tatacgatag
     6901 tcccctttcg gtatcgtggg cacaagtatg gaatgtaact ttcgacaaat ctttttttac
     6961 acaattaaac gaagcctcgc tcaaaaccat ttattgtttt ctgggatatt acaatgtgtg
     7021 tcatcttgga ggcgaaatta agaacccaca aaaaaacatc ccccgcagta tttttatttc
     7081 tattgccatt atcacttgtt tgtatttggc catgcagtgg agtgttcttc gagtaatttc
     7141 tgtaaacgaa gcagcaagcc acgaatatgt tgtgagcatg ttcttcgaaa aaatatataa
     7201 ccgccaggtg gcgctatttg caacgggtgg tattttgatt gtggcttttt cttctttgtt
     7261 tgcggtggtg ctgggctatt cgcgtgtgcc ttatgcagca gcggttgatg gaaattattt
     7321 taaaatattc gccaagcttc acccacgttt aaatttcccc tacatgtcat tattaattct
     7381 tggaagcata gcttttgtat ttagcctact tttcaaaatg agagaaatta tttcggccat
     7441 cattgtcgta cgaatattaa ttcaatttgt ttcgcaggct ttgggcatta ttattttcaa
     7501 taaaaatatt gtgcagcaat ctcccacatt caaaatgcca ctttttcctt tgcctgctat
     7561 tcttagtata attatatggc tatatatttt tggctggagt caatgggtgt atattgcagg
     7621 agcatttgtt ataattatta caggtgttat tttattttta attttgagtt accagcaaat
     7681 gaaatggccg tttttaaaaa atgaaaaaag ataattttaa aaaaaatacg cacgaaaaaa
     7741 atatcacaaa aggttttggc gtgccaaaag gtgagcccga taaaaaaacc gcttttgata
     7801 ttattggtat tttaataatt gtgttgttag gcatcattat ttattccaac acttaccatt
     7861 gtacgttcca tttagacgat gctcatacaa tagttgataa tagcgcgata aaagatttat
     7921 tcaatatcaa agctatttgg aataacaatc acaatcgctt tatcccttac ttatcctttg
     7981 ccatcaatta tcacttcggt aagctcgatg taacgggcta ccaccttgtc aacattatta
     8041 ttcacttaat gaatgcatgt gtggttttca tgcttactaa actgttattt tcaacggttg
     8101 tgcttaagca aagtgttatt gctcaacata aaaatatctt tgctttagta actgcattac
     8161 tattcgtttc gcatccgctg gccacacaat cggtaacata tattgtacag cgaatggcct
     8221 cgcttgtaac tctgttttat ttactaacag taaccctata cgtaaaaggc cgaataaccg
     8281 aaaacaataa tttttcaaaa ttcgcctggt tttcaggttc tataattatg gcgatgcttg
     8341 ccatgctttc aaaagaaaat gccatcacat tgccacttgc cataataatg atagaaatat
     8401 tttttctaca atcgaaaaaa acttttttaa tcttgaaaga ttatcgcata gtcgtattgt
     8461 caatattgtt catctccatc attttatttg ttgcttttag gttttcctcc ggcattttta
     8521 atcctctccc acccgatgcc ataaccaatt ttgttgagat aactccagcc aattttctgt
     8581 tcactcagtt tagtgtattg ttgaaatact tacaactgct tttatttcct atcaatcaaa
     8641 atatcgatta tgacataact ttatcgacaa gttttttcga aataaaaacc cttatcagtt
     8701 ttattttact tgccgctatt ttggttttgg gtgtgttatt atttaataaa agtagaatta
     8761 tttctttcgg tattttttgg ttttttgtaa ccattgctgt tgagtcaagc ataattccta
     8821 tttccgattt aatatttgaa caccgcactt atttgccttc ttatggtttt atgttaatac
     8881 ttactacggc catatttact ttgctatggc ccaaaaacaa aaattttgcc ttggccattt
     8941 ttggacttat aataatttcg aacgctgtac taacgtatca aagaaataaa gtatggcaag
     9001 atgaaattac actttggaca gacgctatta aaaaatcgcc aaacaaagca cggccatatg
     9061 taatccgggg caatgcgtac gaagatctta ataaaatgga tgatgccatt ttggattata
     9121 acaaagccat tgaattgcag ccaaactata ctttggcatt cacaaaccgc ggttatgcgc
     9181 ataaacataa aggaaattgg caaaaagcaa tacaagatta taacaaatct atttctttga
     9241 acccgttaaa tgcgttagcg tataccaacc ggggtgtaat ttacggtaac cttaaacagt
     9301 gggataaggc tattgaagat tacacagcgg caataaaagt tgacaatcga tttgtgcttg
     9361 catattctaa tcgtgctatg gcatacgcta ctttgaagca atgggacaat gctatagttg
     9421 actgttcaca agccatagcg atagacccat acaaatcggg tgcttatgta aatagaggcg
     9481 tggcatacca ggcactggga aagattgata ttgccattgc cgattttaca aaagcgattg
     9541 aactaagtcc gcgcaatgta aattactata ttaatcgtgg ccgaatctat ttagagcaac
     9601 gtgagtggaa taaagctatt aatgatttca cggtggcatt gcaattggag ccaaaaaatg
     9661 cgaaagcatt gaaagcgcgc gaatacgctt ataaacaatt gaatattaaa acgaattttt
     9721 agtgtatttt tcactttaat ttatactatg aaaaaaaact tttcatttaa ttctattttt
     9781 ttgttttttg cgattttaaa cactttgcaa cttacaattg atactatatg ttgcaaaaat
     9841 aatttcagga tttttaaaaa attttatgtc cgataacaac aataaaaacc gcgaatatgc
     9901 aaacttacct ttgcatgtat atgcagcagg caagcaaccg ggaatagtaa aaactttgct
     9961 aacaatagcc aaggagtttc ctgcggcaca ttctctcggg tatcgttttg caaaacgaaa
    10021 cataaaggcc cgctatactc aaagcatttt aggcattgtt tgggctttgc ttccaaatat
    10081 ttctattgca gctgtttgga ttattctcca taataataag attgtaactg taagtcacac
    10141 tggaggcaat tatgctttgt ttgtaattat cggaataatg ctttgggccg tatttacaaa
    10201 agcggtactc acccccttac aaatcattca atcgaaccgt ggcgtgttgg tgaaaataaa
    10261 ttttccgcgc gaggcattag ttatactttc attttacgaa actctattca atgcagttat
    10321 aacactaatt gttatcgttg cttcaatgat atattttcaa gtacctgtat catttagctc
    10381 cttgttattt ttcccatcct tacttttact taatattatg ggattatgta ttggcacttt
    10441 gctggttccg gtttcggtat taaacaatga cgtaaatttt ttgttgccta cttttttaca
    10501 atttgctatg tacctcaccc cggtagttta tgcacaaact acctccaatg gtattttgca
    10561 aatactatca ctcaaccccg taagtccaat attaaccatt gcacgcaatt ttctccttgg
    10621 cattgattca ccaatcagtt ggatacaact actaattgta actcttgtaa ctctagtatt
    10681 atttattgca ggaataataa tacaacgctt tgctatggga atattaatcg aaagaatggg
    10741 aagttaaact atgagtgaga atacagaaat attagttgat gtaaaaaacg tatcaaaaaa
    10801 attcagcctg aatttgcgta caagtatgat gtatggttta aaagaccttg gcaaaactat
    10861 gctgggcata ccatacgatt catcgcaatt gcgcgaaaag gaattttggg ctgtaaagga
    10921 tattagtttt caattacgca gaggggaatg cttagggctc ataggccgta atggtgcggg
    10981 caaaagcacc ttgctaaaat tgttgaatgg attgatacgc cccgatgaag gatcgataac
    11041 aatgcgtggc aaagttggag cacttataga attgggtgcc ggatttaatc cactcctttc
    11101 gggccgcgaa aacgtttata acaatggaca actatta
//
LOCUS       DAORQA010000046        11048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1595_length_11048_cov_8.955335, whole genome shotgun sequence.
ACCESSION   DAORQA010000046 DAORQA010000000
VERSION     DAORQA010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11048
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1595_length_11048_cov_8.955335"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>710)
                     /locus_tag="PK736_04485"
     CDS             complement(<1..>710)
                     /locus_tag="PK736_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRC32679.1"
                     /translation="VVAAGGTSPYTYLWSNGKTTKYIIGLAAGTYTVTVTDANGCTKT
                     LSNSVGQPAALLALYTVTNVSCNGGSNGGVTLTVTGGTSPYKYLWSNGTTTKNLSGVI
                     AGIYSVTVQDKKGCIINATAVISQPAAIIISMTPKSPKCNGAATGQATATVTGGTSPY
                     TYLWSNSATTATATGLAAGTYTVTVTDNKGCTKTASVTITQPAVLACTATVIQGILCN
                     GGTGKAGVTATGGTPGYT"
     assembly_gap    711..751
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<752..>11048)
                     /locus_tag="PK736_04490"
     CDS             complement(<752..>11048)
                     /locus_tag="PK736_04490"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRC32680.1"
                     /translation="TEDYNVNIVNPLFYSWSPATGVSSTTVLTPTITPPLVGIGPTTT
                     VYTLVTTDAAGCTSSNTVTLTNNGPADVAPTGVGANRCGPGTVDLTATGLSTLNWYTV
                     PTGGSIVGTGSPFTTPSLSTTTTYYVESFNGNCGSPRVAVVATINAITPAVIVAPVTA
                     ICNGGSATLSVDTGTTITFSQVLTGGASTLAQCAIWDAFTNSLVPAPYTKVKMWGDNN
                     PAAIAGREVTNPATVQAIAAALRTRAAEVNFPAADQFGNIWRVGLSCGSGCGNPIATV
                     ELNADNNGTDCSCITPGWAIRPNLTGSNSGGINTATCGGPTQTVNLQFTSAVSISSYA
                     WSPATGLNCTDCNTVIANPSVTTTYTLVTTTPNGCTSSATVTITVETPPSAPTASVVS
                     VPCGNGNAVLSATALGGGTLKWFANATGGIALAPNGSPRSFAISTDTTFYVSETTLAA
                     PFCEGPRTPVFVDIIDPDTIAATVDVPLVCGFSPGAFQVNLSVANVSSPQNSQYFYTW
                     TAPGSGGLNSSTGTAVTANPTATTTYTVTATQIGNLTVNINGVSPNGSTTQDEITWTL
                     KDASNVTILSGGPYAGQNNNVTSAPVLSTNGPFTFDISAGINTDFTNYTVFCEGNAVA
                     SGCVRGTANTAQTECATFTPVTVPNINGCFGSCNRITTVLVGVGANPTVAPTGVITIP
                     GCDSASIQLNANAVSSNFVSVNANIGTGTIQNSSTTEPAPYGNFWGSNRSQMLIRASE
                     LSAAGLVNGSSLTALSFDVVNTNGTAALDQFTISLGLTPALALTAGVPIATPFTVVSP
                     ATYTPVNGVNTHTFSSPVIWDGTSSIVVNTCFFNPAFTSNCTFNQTATAFVSTTNIRA
                     DGASQCALNPGTAFSQRPNIVFTATAGTTVSVSWSPSAGLSSSTIQNPTAKIAGTTTY
                     TVTATENGSGCSTTGSVTVTFNPTTIPPAPTVTGETICGPDTVHLTASSLDTINNLII
                     WTDTIGGQILNFGNTYNPYVTQSGSYYVHEIPPYSSPTLGNNIGASAGFFPDGASFMN
                     FTVTAPGGIIINTVDVSTNVPAMAPLVIALVSSTGQIVSILDTIFINGGTTALQTISL
                     NLYVPQGTWRLQPILNPNLNVSNTSTLPSTIPGQITVSCFGNVSNACFPANFNGLFYN
                     WVVNTACISPAAIVNYTVNAPPPVGIASSNGISFCDSVNTVLTVVDSTFSGYDVFSWS
                     PATGLSATTGASVSVAGLLGTTTYTLTASNSLSGCNNVQTITLTLNPAPTLSVSYRDT
                     TICPLAVPVLPFNATAAKSSIVPIGNRFSPNFVVGTLYGGNTTQYSQMIFTAAELNAA
                     GLVGPTNITSIAYFVANKLTTTTYNIDIKMRADVSIPAAFPSTAHINTGLTTVFTNPA
                     LASTLGWNTYNITPFFWDGVSNLLVSQCFTHNIAGFFDVVNTSPTTGVRYSATNAAAC
                     GAATGAFIPAPNANDRPNVRFTGGNVLFSWSPALGLSGTTIEDPTMTAALIGNNDTSI
                     TYHLTVTDPLSGCAKTDSITVNLSTTPLNPIVHYISDSVFCLGGNASMYVNGTTGDFQ
                     WQISSDGINFTDIPGQTNDTLTQAITNTTYYRVDVSCTADSINSSTMTFVVNNPAIIS
                     VVGDTICGVGQVCLSAVKNDPAYFVQWFNDSTGGNVLASGDTFCTVISATDTFWVQAS
                     VDTNSLSASGNIFYMRGSGGEPWASATNVTALNAVYGPQGTAWNLVDYLAAVPANIFN
                     STTKIVFMEGSDASITQFQTFYTANLALIESWVSAGGHLFVNDGPNGRPGTVPLVFGV
                     TTLTNSQFTPTATIAAGQSGHPIFNTPLSGLGSSWTGGSYGHAVITGGPSTKLIDGIG
                     GASVLSVLDYGNGRVLFGGMTMTNFHSPLPQADNLRRNMLSFLNTGFNACVSTPRIPV
                     VAVSTPPPDIALVQSSTTICELTCMNLDVDASGIANYQVFNWSPAAGLSATTGASVQA
                     CPLVTTTYIVTAFDTVSGNACSNSDTVVITVTPAPTVSITTTGNPSCGIDSSQITASV
                     IPFIPPTGQYSVTSIPFAPVTPTGTPVVLQNAGTMVTPLSSGSADDGTWQNIPLPAGF
                     AFNYYGSTPTAMSVSTNGFLTFTSNSGLSGCCSGQILPNATTPNDVIAYGWEDWNQTA
                     GTTDFYTEGTAPFRKFVLRSQGIPRFGGTGAPSTVMVVLNETFNTVEIQIASIVCGPS
                     DATTLGIENGAGTLGTAVAGRNSVVGWTASNEGWLFAPPIASGINYAWSPSAGLSSTT
                     IANPMAQPTGTTVYTLTVTETGTGCTRTSTVTVVNSALPQPTIAPGDSVGCAGTFSDY
                     WLYAVDTGAYAGGYPPGTLFDWSINLGGPVADLDSILLDLNNLGNITLTVTLPPSLGN
                     NCSGTTSINVNFSEPVQTAYMSQDSVSCTPNNDGVAYVYHLEANGPIRYVWVDALGDT
                     VRDVTNNYNYAGLFDADLDGFLDDRNGDFILDILDNYNIFKDSLQNLIVGTYTVYITT
                     NAGDPYPTPQCVSSGTINIGRTPCVPPQLLDPCVCVDNADVSRRAPYTDGQFSETISL
                     LGGQSPWTLVSLTSSSGKPLSNTPAIEDCIDNTTQTTPVTTADIVEVLPNDSSYVNFY
                     LFDGDTYTLVVEDASGAQLTISGGGCNYPEAPINDTTITVCADNFGLNIQDAAFPGGV
                     DPSILLSWYDDSLCTGSPLMYGDLSNVPANYPDLTDISNPGPGGNIGYTVYNFYVASD
                     SVADVSCEGKRAMVTILSNEVVATVDSIVNVNCNGEASGAIYITMTNGTAPFTYAWSN
                     GSTEEDLTNLVAYSYDLVVTDSLGCTFEMSYDITENPQFTIGKNIINNQCHGDSTGQI
                     LLFVSGAVPFTNGAPYSYLWSDGQTTNIANNLIAGTYTVTVTDSVGCDTILTFQIFDP
                     TLLDQTHTIIQPLCNGGTGDITVNAFGGTPWTGLGVHPAGYEFSLDGGTSQTSGVFTG
                     IAGGNHTIAITDSNSCITYLIFNINEPAAMVCGGAVTNVMCNGGNNGSITANITGGTG
                     NYGYLWNDGSTNQTINGLTSGAYTVTVTDGNGCTIQCTYTVTEPSPITFTATACANVS
                     CFGGNDGKACVTNVLGGSPPYTYQWNTLPAQTTAQATGLTAGTWTVTVLDAMGCIATG
                     DVIITQPSAALSITGTVTNTTCNGGNNGAINITVTGGTAPSTLLWSNGNTTTSNTGLV
                     AGTYTVTVTDANNCTLTASYTVGQPTAIAISLVSTNVSCNGGGNGSITTNVSGGTGAY
                     SYAWSNGGSTSGVSGLAAGIYTVTVTDANGCTTSASATITSPSALGCICNSNVTNVSC
                     FGGSNGSVTAQPLGGTAPYSFVWSTGATTQTISSLAAGTYTVNITDANGCTKVGTATI
                     TQPNALVLTVTGNNLTCNGNGGGSATASISGGIAPYTYLWTPTGQTTATATGLAAGSY
                     TVQVTDANGCTVTGQVTLTQPAPM"
BASE COUNT         2959 a         2387 c         2434 g         3227 t
ORIGIN      
        1 ggtatagccc ggtgtgccgc ctgtggctgt tacacctgct ttgccggtac ctccgttgca
       61 caatatgcct tgtatcactg tggctgtaca ggcaagtact gccggctgcg ttatcgttac
      121 acttgctgtt tttgtacagc ctttgttatc ggttactgtt actgtatacg taccggctgc
      181 aaggcctgtg gccgttgccg tagtagcact gttcgaccat aagtatgtat aaggtgaggt
      241 gccgcccgtt actgtggctg tggcctggcc cgtagctgca ccattacatt ttggactctt
      301 tggtgtcatg ctgatgatga tggctgctgg ctggcttatc actgccgtgg cattgatgat
      361 acaacctttc ttatcctgta ctgttacaga ataaatacct gctattactc cgctcaggtt
      421 tttagtagtg gtaccattgc tccataaata tttgtatggg ctggtgccgc ctgttaccgt
      481 gagcgttact ccgccattgc tgcctccgtt acaacttacg ttggttactg tatataatgc
      541 taacaatgct gcaggctgac ctacgctgtt tgacagcgtt tttgtacagc cgtttgcatc
      601 ggtaactgtt actgtatacg tgcctgctgc aagaccgata atatattttg tcgtctttcc
      661 attgctccac aaatacgtat aaggtgaggt gcctccggct gctactactg nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncattggcgc aggttgggta agggttacct
      781 ggcctgttac cgtacatcca ttcgcatctg ttacctgcac tgtgtagctg cctgctgcta
      841 agcctgtggc cgtagcggtg gtttgacctg ttggtgtcca caaatatgtg taaggtgcta
      901 tgcctccgct tatcgatgct gtagcactgc ctccgccatt gccattgcag gtcaggttat
      961 tccctgttac tgttaatacc agcgcattgg gctgtgtgat ggtggccgtg cctactttcg
     1021 tacagccgtt tgcatctgtt atgtttacag tataagttcc tgctgccaaa ctgctgatgg
     1081 tttgggtagt agctcctgta ctccatacaa aactataagg tgcggtgccg cctaatggtt
     1141 gtgcggttac actgccattg ctgccaccaa agcaacttac attggttaca ttgctgttgc
     1201 atatacagcc tagtgctgat ggtgatgtta tcgttgcgct tgcacttgtt gtgcaaccgt
     1261 tggcgtctgt taccgttacg gtataaatac cggccgcgag gccgctaaca cccgaagtgc
     1321 tgcctccatt gctccatgca tagctataag ctccggtgcc tccgcttaca ttggtcgtga
     1381 tgctgccatt accgcctccg ttgcagctta cattggtact taccagtgat attgcgattg
     1441 ccgttggctg gcctacggtg tagctggctg ttaatgtgca gttgtttgca tcggtaactg
     1501 ttactgtata cgtaccggcc acaaggcctg tattgcttgt tgtagtgtta ccgttgctcc
     1561 acaatagtgt agagggcgct gtgccgccag ttaccgttat gtttatcgct ccattgttac
     1621 ctccattgca ggtggtattg gttactgtgc cggtgatgct taatgcggct gatggttgag
     1681 tgatgattac atcacccgtg gctatacatc ccatcgcatc caatacggtc actgtccacg
     1741 tgcctgccgt taagcctgta gcctgtgctg ttgtttgtgc cggtaaggta ttccactgat
     1801 aagtgtatgg cggactacct cccaatacat ttgttacaca tgccttacca tcgttgccgc
     1861 caaagcagct tacattcgca catgcagtgg cagtaaatgt gataggcgat ggttcggtta
     1921 ctgtatatgt acattgtatt gtgcagccat tgccgtctgt tactgttacc gtatacgcac
     1981 ccgaggttaa tccgtttatc gtttgattcg tgctgccatc attccacaga taaccgtaat
     2041 tgccggtgcc acctgttatg tttgctgtga tgcttccatt gttgcctccg ttacacatca
     2101 cattagttac agcccctcca caaaccatag ctgctggttc atttatgttg aaaatcaaat
     2161 aagtaataca actgttgctg tctgttatgg caattgtatg gtttccacct gcaatacccg
     2221 tgaaaacgcc actggtttga gaagtacctc catcgagact aaactcataa ccagcaggat
     2281 gaacgcctaa gccagtccat ggggtaccac caaatgcatt tacagttatg tctcccgttc
     2341 cgccattaca taacggctga ataatagtat gagtttgatc gagtagtgtg ggatcaaata
     2401 tttggaaagt taatattgta tcgcatccta cgctgtcagt aacagttact gtatacgttc
     2461 ctgctataag attgttggca atatttgttg tttggccatc actccataaa taactgtaag
     2521 gcgctccgtt tgtgaatggc actgcaccac ttacaaacaa taagatttgg cctgtactat
     2581 caccatggca ttgattattg ataatatttt tacctatggt gaactgtgga ttttctgtta
     2641 tgtcgtaaga catttcaaac gtacaaccta atgaatcggt aacaactaaa tcataagaat
     2701 atgcaaccaa attagtcaaa tcctcttcgg tagaaccatt gctccaggca taagtgaatg
     2761 gagcagttcc attagtcatg gtaatataaa tggctccgga ggcttctcca ttacaattta
     2821 catttactat tgaatccact gtagccacaa cttcgttcga caatatcgtt accatggcgc
     2881 gtttaccttc gcaagaaaca tctgccactg agtctgaagc cacatagaag ttatataccg
     2941 tgtagcctat gtttcctccg ggacccggat ttgatatatc tgttaagtca ggataatttg
     3001 ccggtacatt agacaagtct ccatacatta atgggctgcc ggtgcacaat gaatcatcat
     3061 accatgataa taatatcgat gggtctactc ctcctgggaa tgcggcatct tgtatgttta
     3121 atccgaaatt gtcggcacat accgtaatcg tagtatcatt tattggtgct tcaggataat
     3181 tgcatccgcc tccgcttatt gttaattgag cacctgaagc atcctcaact accaacgtgt
     3241 aagtatcacc atcaaacaaa tagaaattta catatgagct atcgtttgga agaacttcaa
     3301 caatatctgc agttgttaca ggtgtagttt gtgtggtgtt gtctatacaa tcttcaatcg
     3361 ctggggtatt cgataatggc ttgcctgatg aagaagttaa actcaccaat gtccatggtg
     3421 attgacctcc caataaactg attgtttcac tgaattgtcc gtctgtataa ggtgcccttc
     3481 tgcttacatc ggcattatct acacatacac atgggtctaa taattgaggt ggtacacatg
     3541 gtgtacggcc aatgtttata gtacccgatg atacgcactg aggtgttgga tatgggtcgc
     3601 ctgcattggt ggtgatataa actgtataag tgcccactat caaattctgc aacgaatctt
     3661 taaatatatt ataattatca aggatatcaa gaataaagtc tccatttctg tcatcgagga
     3721 aaccatctaa atcggcatcg aacaatcctg cataattgta gttgttagtt acatcacgta
     3781 ctgtatcgcc taatgcatct acccaaacat aacggatggg gccattggct tctaaatgat
     3841 atacgtaagc aacgccatca ttattcggag tgcaacttac gctgtcctga ctcatataag
     3901 cagtttgaac aggttcgcta aagtttacat ttatacttgt agtacccgaa caattgtttc
     3961 ctaaacttgg tggcaatgtt actgtaagtg ttatatttcc taaattattc aaatccagca
     4021 aaatagaatc taaatcggca actggtccac caagatttat tgaccagtcg aataatgttc
     4081 cgggtgggta gccgcctgca tatgcacctg tatcaactgc gtataaccag taatcgctaa
     4141 acgttccggc acatccaacc gaatcgcctg gggcaatggt tggttgtggc aatgctgaat
     4201 ttactacagt tacagttgat gtgcgggtac aacctgttcc ggtttcagta acagttaatg
     4261 tgtatacggt cgtaccagtt ggctgtgcca ttggattggc tatcgttgtg gaacttaatc
     4321 ccgctgaggg tgaccacgca tagtttatgc ctgaggctat aggtggtgca aataaccatc
     4381 cttcgttgga cgctgtccaa cctaccactg aatttctacc agccacagcc gttcctaagg
     4441 tacctgctcc gttttcaata cctaatgtag ttgcatctga cggaccgcac actatggatg
     4501 ctatttgtat ctctacagtg ttaaatgttt cattcaatac taccatcact gttgatggtg
     4561 ctccagtgcc tccaaatctt ggtatgcctt gtgagcgcaa tacaaactta cggaaaggag
     4621 ctgtaccttc tgtatagaaa tccgttgtgc cggctgtttg attccaatct tcccagccat
     4681 aagctattac atcatttgga gtagttgcat ttggcaatat ttgacctgag caacagcctg
     4741 ataggccact gtttgatgtg aatgttaaga atccatttgt tgataccgac attgccgttg
     4801 gtgtgcttcc ataataatta aatgcaaaac ctgccggcaa aggtatattc tgccaagtgc
     4861 cgtcatcagc acttccactt gaaagtggag taaccatagt accggcattc tgcaatacaa
     4921 ctggtgtacc cgttggcgta accggtgcaa acggaattga agtaaccgaa tactgaccgg
     4981 taggtggtat aaacggtatt acactggctg ttatttgcga actatcgatt ccacaacttg
     5041 gattacctgt agtggttatc gatactgttg gtgcaggtgt tactgttatc accaccgtgt
     5101 cggaatttga acatgcattg ccacttacgg tatcgaacgc tgtaactata tatgtggtag
     5161 ttaccaatgg acatgcctga actgacgctc ctgtagtggc acttaagcct gccgctggag
     5221 accagttgaa cacctgataa ttggcaatac cacttgcgtc cacatccaag ttcatacaag
     5281 tcaactcgca aattgttgtt gaagattgta ctaatgcgat gtccggtgga ggcgtagaaa
     5341 ccgccacaac aggaatacga ggagtactaa cacatgcatt gaaaccggta ttcaaaaacg
     5401 ataacatatt tcttcttaaa ttatctgctt gtggtaatgg tgagtggaag ttggtcatag
     5461 tcataccacc aaacaataca cgtccatttc cataatccaa tactgataat actgaagcac
     5521 ctccaattcc atctatcagt ttggttgaag gacctcctgt aattacagca tgtccgtaac
     5581 taccgccagt ccaagaagaa cctaaacctg aaagtggtgt gttgaaaatc ggatgaccac
     5641 tctgacctgc agcaatagta gcagtaggag taaactgaga attggtaagc gttgtaacac
     5701 caaaaactaa gggcacagtg ccagggcgac cgttaggacc gtcatttaca aataagtgac
     5761 cgccagctga tacccatgat tcaattaatg caaggtttgc agtatagaat gtttggaact
     5821 gagtgatgct ggcatcacta ccttccataa atacaatctt agtagtagaa ttaaaaatat
     5881 ttgcaggaac agcagcaaga taatcaacta agttccatgc agtgccctgc ggaccgtaaa
     5941 cagcatttaa tgcagttacg tttgttgctg atgcccatgg ctcaccgcct gaaccgcgca
     6001 tataaaatat attacccgat gccgaaagcg aattggtatc aacacttgcc tgaacccaga
     6061 aagtatcagt tgctgatata actgtgcaga aggtatcgcc actggcaagt acatttccac
     6121 ctgttgaatc attaaaccac tgaacaaaat aagcggggtc atttttaact gcagatagac
     6181 atacttgacc aacaccgcaa atggtatcgc caacaacact gatgattgca ggattgttaa
     6241 ctacaaaagt catggtgctt gaatttatag aatcagctgt acatgaaacg tctactctgt
     6301 aataggttgt gttggtaatc gcctgagtaa gcgtatcgtt tgtttgacct ggtatatcag
     6361 taaagttgat gccatcagaa gatatttgcc attggaaatc accggtagta ccattaacat
     6421 acatacttgc atttccgcct aaacagaata ctgaatcgct gatgtaatgt acaattggat
     6481 ttaatggagt tgtactcaga ttaacagtta ttgaatctgt tttggcacag ccacttaacg
     6541 ggtcggttac tgttaaatga tatgttattg aagtgtcatt attgcctatc aaggcggcag
     6601 tcattgttgg gtcttcgatt gtagtacccg ataatcctaa agccggtgac catgagaaca
     6661 atacgttacc accggtaaac cgcacattgg gacggtcgtt agcattaggc gcaggaataa
     6721 atgctcccgt tgcggcacca caagccgcgg cattagtagc ggaataacgc actcctgttg
     6781 ttggcgatgt atttactaca tcgaaaaatc ctgctatgtt atgtgtaaaa cattggctaa
     6841 ccaaaagatt actcacccca tcccagaaaa atggtgttat attataagtg ttccaaccga
     6901 gtgtacttgc caatgccgga tttgtaaata cagtagttaa gcctgtattt atatgtgccg
     6961 tacttgggaa tgcagcaggg atggacacat cagcacgcat tttaatgtca atattgtatg
     7021 ttgtagttgt aagtttattt gcaacaaaat acgcaatcga tgttatattg gtaggaccca
     7081 ccaaaccggc agcatttaac tctgctgcgg taaaaatcat ttgcgaatat tgtgtggtat
     7141 tgcctccata aagtgtgcct acaacaaaat taggactaaa cctgtttcct attggcacaa
     7201 tactcgattt tgcagctgtt gcattaaaag gtaataccgg cactgctaac gggcatatgg
     7261 tggtatcacg gtagctcacc gataatgtgg gtgccgggtt caatgttaat gttatagttt
     7321 gcacattatt acaaccactt aagctgttcg atgctgttaa agtgtacgtg gttgtaccta
     7381 acaaacctgc aacactaacc gatgcgcccg ttgtggcact taagcctgtg gccggactcc
     7441 atgaaaatac gtcatatccg ctaaaagtgc tatcaacaac tgtcaatact gtatttacag
     7501 agtcgcagaa agatatacca ttactcgagg caatgcctac gggtggcggt gcatttactg
     7561 tgtagtttac tatggctgcc gggctaatac acgcggtgtt cactacccag ttataaaata
     7621 agccgttgaa gtttgctgga aaacatgcgt tgctaacatt accaaagcat gaaactgtaa
     7681 tctgcccggg aattgtactt ggcagtgtag aagtattact tacgtttaga ttaggattta
     7741 aaatcggctg caacctccat gtgccttgcg gtacatataa gttgagcgat atggtttgca
     7801 aggctgtagt gccaccgtta ataaatatgg tgtccaatat cgatactatt tgacctgtag
     7861 aacttaccaa cgctattacc aagggtgcca tggccggtac atttgtcgat acatctacag
     7921 tgttgattat gataccgccc ggtgctgtga cagtaaaatt cataaatgat gccccatcgg
     7981 ggaagaaacc tgcgcttgca ccaatattgt tgcccaaagt cggactcgaa taaggtggta
     8041 tttcatgtac gtagtatgac ccactttggg ttacatatgg attatacgta ttgccaaagt
     8101 tgagtatttg tcctcctatt gtgtcggtcc atattattaa attatttatc gtatcgaggc
     8161 tcgatgctgt aagatgaacc gtatcgggtc cgcaaatagt ttcgcctgtt accgttggtg
     8221 ccggtggaat ggtggttggg ttaaaggtga ccgttacact tcctgtggtg ctgcaacccg
     8281 aaccgttttc agttgctgtt accgtataag tagtggtgcc ggcaatcttt gccgttgggt
     8341 tctgtatagt cgaactgctt aaaccggccg atggactcca acttaccgat actgttgtac
     8401 ccgcagtggc agtaaatact atatttggtc tttgtgagaa agcagtaccg ggattcaaag
     8461 cacattgcga agcaccatca gcacgtatat tcgttgttga aacaaatgca gttgccgttt
     8521 gattaaatgt gcagttgcta gtaaacgctg gattaaagaa acacgtattt acaacaatac
     8581 ttgatgtacc atcccatatt accgggctgc tgaatgtatg cgtattaaca ccattaacag
     8641 gtgtataagt agcaggagat acaactgtaa aaggagttgc aataggaaca ccagccgtta
     8701 aagcaagagc aggagttaat cctaatgaaa tagtaaattg atccaaagca gctgtgccat
     8761 tggtatttac aacatcaaaa gacagtgcag ttaagctgct gccatttacc aaacctgctg
     8821 cacttaattc agaagcgcgt atcagcattt gacttctgtt actgccccaa aaattgccat
     8881 aaggagcagg ctctgtggtg gacgagtttt gtatcgtacc ggtgcctatg ttggcattta
     8941 ccgaaacaaa attacttgaa acggcattcg catttaattg gatgctcgcg ctatcgcaac
     9001 ccggtatggt gataactcct gtaggagcaa ctgtaggatt tgcacccact cctaccagta
     9061 cagtggtaat tctgttacaa gaaccgaagc aaccgttaat atttggaacg gtaacaggtg
     9121 taaaagttgc acattctgtt tgagccgtgt tagctgtacc tcttacacaa cccgaagcaa
     9181 ctgcatttcc ttcgcagaat actgtatagt tggtaaagtc ggtgttaata ccggctgaaa
     9241 tatcgaaagt aaatggtcca ttagtagaaa gtacaggtgc cgatgttaca ttattatttt
     9301 ggcctgcata aggtccgcca cttaatatgg taacatttga tgcatcttta agtgtccagg
     9361 taatttcatc ctgggtggtt gagccgttcg ggctaacccc attaatattt actgttaaat
     9421 ttcctatttg agttgctgta actgtataag tagttgtagc cgtgggattg gcagttacgg
     9481 ctgttccagt acttgagttt aagcctcccg atcccggtgc tgtccatgta taaaaatatt
     9541 gactattttg aggcgaagaa acatttgcta cacttagatt aacctggaat gcacccggtg
     9601 aaaatccaca aactaatggt acatcaacag tagcagcgat agtatcagga tcaataatat
     9661 ctacaaatac aggtgtacgt ggtccttcgc agaaaggtgc cgctaaagta gtttctgaaa
     9721 cataaaatgt ggtatcggtg cttatagcaa atgaccttgg tgagccattt ggcgcaagcg
     9781 cgattccgcc tgttgcattg gcaaaccatt tcaatgtacc acctcctaat gctgttgcac
     9841 ttaatacagc atttccgtta ccgcaaggta cagaaacaac agaagcagtt ggtgccgaag
     9901 gaggcgtttc aacagtaata gtaactgtag ccgatgaagt acagccattt ggagtggttg
     9961 ttactaaagt ataagttgtt gtaacacttg gatttgctat aacagtatta cagtcagtac
    10021 aatttaaacc tgttgcaggt gaccatgcat aagatgatat ggatacagcc gatgtaaact
    10081 ggagattaac tgtttgagtt ggaccaccgc aggtggctgt attaatacca cctgaattgg
    10141 aaccggttag attaggtctg atagcccagc ccggtgtaat acatgagcaa tctgtaccat
    10201 tattgtcagc atttaattca acagtagcaa tagggtttcc acaaccggaa ccacatgaca
    10261 aacctacccg ccaaatatta ccaaactgat cggctgcagg gaagttaact tcggctgcac
    10321 gtgttcttaa tgctgctgct atggcctgaa cggttgcagg atttgtaacc tcgcgccctg
    10381 ctatggctgc tggattatta tcaccccaca tttttacctt tgtataaggt gcaggaacca
    10441 acgaatttgt aaaggcatcc catatagcgc actgtgctaa ggtagatgca ccaccggtta
    10501 atacttgtga gaaagtaatt gtagtgccag tatctacaga taatgtagca gaacctccgt
    10561 tacaaattgc tgtaaccgga gcaactataa ctgcaggtgt aatcgcattg attgttgcta
    10621 caaccgcaac acgaggactt ccacaatttc cgttaaatga ctctacataa taggtagttg
    10681 ttgttgaaag agaaggagtg gtaaatggcg aacctgttcc aacaatagaa ccacctgtag
    10741 gaaccgtgta ccagttaagt gttgacaatc cggtagctgt taaatcaaca gtacccggcc
    10801 cgcaacgatt tgcacctact cctgttggcg ccacatcagc cgggccattg ttagttaaag
    10861 taactgtgtt tgaagacgta catcctgctg catcggtagt aactaaagtg taaaccgttg
    10921 ttgttggtcc tataccaact aatggaggtg tgatggtagg agtcagtact gtagtacttg
    10981 aaacacctgt tgctggcgac catgaataaa agagaggatt aacaatgtta acattataat
    11041 cttctgtt
//
LOCUS       DAORQA010000047        10521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1692_length_10521_cov_13.286547, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000047 DAORQA010000000
VERSION     DAORQA010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10521
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1692_length_10521_cov_13.286547"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..>854
                     /locus_tag="PK736_04495"
     CDS             78..>854
                     /locus_tag="PK736_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRC32681.1"
                     /translation="MKKYIIFVLMLVSMVSYGQDPQFTQFYASPLYLNPAFAGTARCP
                     RVMLNYRNQWPALNRAFVTYAASYDQHFDVLSGGIGLILLKDDAGEGTINTTSASFMY
                     SYQANLSRKFSIKAALQATYMQKRINWDKLTFGDMIDPRYGFVYETQEVRPNESKGFA
                     DFSAGILGYSEKFYGGLAVHHLTQPDEAFIVNGASALPMKITAHVGLMLPIAGSSTYG
                     RDKDDGTYLSPNLLYQQQRSFNQLNVGMYFLKSPFVGGLWY"
     assembly_gap    855..912
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1149..2603
                     /locus_tag="PK736_04500"
     CDS             1149..2603
                     /locus_tag="PK736_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRC32682.1"
                     /translation="MNLRKKSIFKLSFSALAVMLVMNSCKQEVSSSTGWNYNDTKNGG
                     FEVVNYVEQETGPGLVLIEGGTFTMGRSEQDMTMDWDNMPRRVTVHSFYMDESEVANV
                     HYLEYLYWTSRVFGTDYPEVYKKALPDTLVWRDRLAFNEPFVEYYLRHPAYQYYPVVG
                     ISWLQATDYCAWRTDRVNERILIREGILRENPAQVNEDNFNTDAYLAGQYEGLVKSPL
                     QDLSPNGDTRKVKMEDGILLPRYRLPTEAEWEYASFGLIGNTKDERIGDRKTYPWNGH
                     YVRNSEEKYKGEMLANFKRGRGDNMGVAGKLNDNADIPAEVISYLPNDYGLYNMAGNV
                     CEWVGDIYRPLSHEDVSDFNPFRGNVFQTFVTDADGNIDEKDSLGRVKYRDVTEAEAI
                     TRRNYKKADNKGYVDGEETEYIRYDENVSLINDRVRVYKGGSWKDRAYWMAPGTRRYL
                     DESLSNDFIGFRCAMIRVGSPSGLGQADKTTRAH"
     gene            2883..7316
                     /locus_tag="PK736_04505"
     CDS             2883..7316
                     /locus_tag="PK736_04505"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-repeat neuraminidase N-terminal
                     domain-containing protein"
                     /protein_id="HRC32683.1"
                     /translation="MKKTIVSKITVLGFAFFLCNVLSTNAQPFTDNFNGPSLTSANAW
                     TSWQSKEMFGYTPKTISAGPVFGNQWFWSNGVGLTPAAPAPFSAGGMIKFNSRNGGLA
                     PAGAIDTGCAYIATPQLDYSGRNGAAANLSFYLWHEAYAGATSDSVVVYASNSNQLQG
                     ATRLGKVWADPAGYGNGWVQSIFAIPNVAQFNGCNKVIFMFVAYTWTSYRNIYVDNIS
                     IDRFPSNMVVQSATLTNQNTNSVASGSTNNQVVAVNINVCGSSTPKQLDSIFFNPTGT
                     NIIADIANAKLFYTGNYPVFSTTQQVFATVNPPINNVTYNNSLGFGNCAAPITLALGD
                     NYFWLTYDIKPQATAGDMVDADFLKCIIHGTCLPGGSIPFGVAQSLPGGLLIGAGYIA
                     PLYTVGTSGNGYTSGDFISSVYVTSAVGNPLFNQEHDITTWLGCTGMSGQYCNRHAPH
                     PTDYTYFAPTNNSITTDRSCSLNLGQGIRSGTVADKIAIAAGNWFSSNYVRAWIDFNS
                     DGDFDDNYVGPGGTIYESIMVNGGYSLSDNNSIASLGTVAGAFPTLKKGDDWNEWNIE
                     VPNDGELPTVGGGSGGPAVHTGYARLRIREVYANGTFTPFSTQANGECEDYDIMLVAS
                     CGIPLVKTCNWLGGGINPNNWFDAANWCPGIPTQYDTAMIQPNVNGNYPIIVSNQNPE
                     CATLRIFSGAQVTVDAPDVVNGNILVPNTGKLKVYHNIEIGEAPGTFAKLNVLQNPVN
                     SVTTGIASSAGTLLLSPFRNDKQDAKIQFIYAPNELAIMGLKSGDVLDKITFYIKSAT
                     VLPSGNLGNFTVKIWQYDSTYSFPALLSPATEVAVNNALPGRIPSTGAINVTTCYGPS
                     NFTVPAINQINTIVPVTINFTAKPSINVSRYLCFEISRDSPFTSGVAYPILYESTLPR
                     YCLVLNNANNNTSSNTIAAKITGVGTAPGSAGVNANNGILNNGNFVDGGVSSPFSYIT
                     GSTYVFRPTSEIGFTRHYSKYNIEVGGNWINNNAPGLPLGFEAGYSAVKMINNYVPGL
                     HPNNDVDTIGGSEVTIFSELEINDAKNVRMNVIGINNGNTGLIVDSTLKLTTGQLQLS
                     NEKVVINNPNQNAIQRTTGFIQSQQTNNYNKVQWNIGNNTGPHTIPFGIAINYLPLTF
                     NLVSGSFGNITASTYYTNAANTPLPVTPEAVTNLCFPSSTNNSSQMVDRYWQLNGTIP
                     GGGVANIEFVYGANELPPGVNDTDLIATRFENDTLGACAGGTWLSNAQMGNPLQQSGS
                     GYGYGTHAVRINNVSNFSPWSLTTKSSFTPPPCIVTGLKVTNATNTTAMLSWAKLSSA
                     TSYRVRIQNQLSNLNAYGVTVYTLDTFTLGNNFLPASAYRFRVAAKCNGVWGAWTAWK
                     NFNTNVPRETTDEINFVDNAKPFLLYPNPASTNITIETGFDNDYQIKIMDTQGRLVYS
                     NSYNAENELLNIDIAKLENGIYQLQIITSENIYLERVVVNR"
     gene            7530..8852
                     /gene="murF"
                     /locus_tag="PK736_04510"
     CDS             7530..8852
                     /gene="murF"
                     /locus_tag="PK736_04510"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRC32684.1"
                     /translation="MNKKNIDTATLHSIFLQCNQKITTDTRKIVAGGIFFALKGDNFN
                     GNSFAIEAVNAGAAYCVVDENINTNDERILYVDDVLKELQNLATFHRQHLNIPVIAIT
                     GSNGKTTTKELFYAVLSQKYNTLATKGNLNNHIGVPLTLLSIMPQHQMAIVEMGANHQ
                     LEIEQLCRIALPDYGMITNVGKAHLEGFGGFAGVIKGKTEMYTHIKNTRGVIFCNAKN
                     EHLYPLIKDYEKLITYLGEASTINAEYYDEGVFLAVEWSKKGESNHNHIRSHLTGAYN
                     FENIAAAITIGDYFEVAAQKINDAIVNYIPDNQRSQVITMPGNINIILDAYNANPTSM
                     YHAIMNFDKKFVTNKVALLGDMYELGADEDAEHLKIIELIAQLNIQQTVLVGKRFAKH
                     KNKLAAHYFDTIANACAFLKTQHFANHNILIKGSRSSQMDKAVNAIVA"
     gene            complement(8865..9830)
                     /locus_tag="PK736_04515"
     CDS             complement(8865..9830)
                     /locus_tag="PK736_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32685.1"
                     /translation="MKNSILSIFALFLFAGIYSANAQNTSKGDGKIYVKILKDIDGKQ
                     ILIDTVFEENDKAAADEFMKQHGVGAPDRRNSNVDINIDGKEMEDALRLMEESLHESG
                     RAIEKMSEELAKELERIHIEVETDDDAYTYQFNFNDLNMEQLQDELNKSFDIKIDTTG
                     QDFSMSFNFKGIDAQTQQEIENSMRKAKEELKQAMENMNDKKVNIKIKIDADKDKIKN
                     EKRIEIKKGKPTKDKANYSAIQKLKIFPNPNKGNFDVTFNTNIVDDLNIQLTDAAGKT
                     ISSENTSQFTGKYERSFNLKNRGKGVYFLKIVFGKETINEKIIVE"
     gene            10050..>10521
                     /locus_tag="PK736_04520"
     CDS             10050..>10521
                     /locus_tag="PK736_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32686.1"
                     /translation="MSIALLGLVVLQAYWIKHDFDLKEKQFDQMVTSSLDATVNKLDM
                     EETFKMMSQAGLINANSFAGMIDSSLAWGVTYEDENDSVALKATDTMGAKQRYGEGTQ
                     MVMEEVKDGKKIKKIIQAYDSTFISKDQSLKINDKRIQSIIQTEPVLANVEDQYK"
BASE COUNT         3322 a         1996 c         2063 g         3082 t
ORIGIN      
        1 gcttagaaat gaaagtgtta attaatattt gcagatttaa attaaataat tttatatttg
       61 ggctgtcaaa gaaaagtatg aagaaatata tcattttcgt gttaatgtta gtgagcatgg
      121 tatcttatgg tcaggatccc cagtttacac aattttatgc atcgcccctt tacctcaatc
      181 cggcatttgc cggcacagcg cgttgccctc gtgtaatgct taactaccgc aatcaatggc
      241 cggctctcaa tcgcgcattt gtaacttatg cagcttctta cgatcagcac ttcgatgttt
      301 tatctggcgg aatagggctt attttattga aagacgatgc tggtgaaggc accatcaata
      361 caacctctgc cagttttatg tattcgtacc aggcaaactt gagtcgcaag ttttcgataa
      421 aagctgcctt acaagcaact tacatgcaaa aaagaataaa ctgggacaaa cttacttttg
      481 gtgatatgat agatccccga tacggatttg tatacgaaac ccaggaagta agacccaatg
      541 aatcgaaagg gtttgccgat ttttcggcag ggatactggg ctatagcgaa aaattttatg
      601 gcggacttgc tgtgcatcac cttacccaac ccgatgaagc ctttatagta aatggagcaa
      661 gtgccttacc aatgaaaatt acagcacatg ttggcctgat gctgcctatt gctggcagtt
      721 ctacctatgg ccgcgataaa gacgatggca cctacctttc acctaattta ttgtaccagc
      781 aacagcgctc attcaatcaa ttaaatgtgg ggatgtattt cctcaaatcg ccttttgtag
      841 gcggattgtg gtatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnaaaaagga atgtttaagg tggggtatag ttatgacgtt accatttcga
      961 aattgagtaa tgccagcggt ggctcgcacg aggtgagtgt ggggattcag ttttattgcc
     1021 gcccaaaaaa gaagcgtttc cgtacaataa gttgccctac attttagtta tacattgtta
     1081 aatttgctgg ggttaattat tttccatatt attgcgtttc gttatctatt cattaattaa
     1141 tattcataat gaatctaaga aaaaaatcga tttttaaact ttcattttcg gcattagctg
     1201 ttatgttagt gatgaattcc tgtaaacagg aggtttcgtc cagtaccgga tggaattaca
     1261 acgacaccaa aaacgggggg ttcgaagtag taaattatgt agagcaggaa actggtcccg
     1321 gtttggtgct gattgaaggt ggcacattta ctatgggccg ctcagaacaa gatatgacca
     1381 tggattggga caatatgccc cgcagggtta cagtacattc attctatatg gatgaatcgg
     1441 aggtggccaa tgttcattat cttgaatatt tgtattggac atcgcgtgtg tttggtaccg
     1501 attatcctga ggtttacaaa aaagcccttc ccgacacatt ggtatggcgc gatcgtttag
     1561 cattcaacga gccttttgta gagtattatc ttcgtcatcc agcctaccaa tattatcctg
     1621 tagtaggtat cagttggttg caagccacag attattgtgc ctggcgtacc gacagggtga
     1681 atgaaagaat tcttattcgc gaaggaatat tgcgcgaaaa ccctgcacag gtaaatgagg
     1741 acaactttaa taccgatgca tacttagcag gccaatacga aggcttggta aaaagcccac
     1801 ttcaagacct tagcccaaat ggcgatacac gtaaagtaaa aatggaagat ggaatattat
     1861 tgccacgcta tcgcctgcca acagaagcgg agtgggaata tgcatcgttt ggattgattg
     1921 gaaatactaa ggatgaaaga ataggagacc gcaaaacata tccatggaat ggccattatg
     1981 tgcgtaattc cgaagaaaaa tataaaggcg aaatgcttgc caactttaaa cgtggacgcg
     2041 gtgataatat gggggttgcc ggcaagttga atgacaatgc cgacatacct gccgaagtaa
     2101 tatcttactt gccgaacgac tatggtttat acaacatggc cggcaatgtt tgcgaatggg
     2161 ttggtgatat ttatcgccca ttatcgcatg aggacgttag cgactttaac cctttccgtg
     2221 gtaatgtgtt tcaaactttt gtaaccgatg ccgatggaaa catagatgaa aaagacagtt
     2281 taggccgcgt gaaatatcgt gatgtaaccg aggccgaagc catcacccgc agaaactata
     2341 agaaagccga taacaaaggt tatgttgacg gtgaagaaac agaatacata cgttatgacg
     2401 aaaacgtttc acttataaac gaccgtgtac gtgtatacaa aggcggttcg tggaaagacc
     2461 gtgcatactg gatggcacct ggtaccagaa gatatttgga tgaatcctta tcgaatgatt
     2521 tcataggttt ccgttgtgca atgattagag ttggcagccc atcaggtttg gggcaagccg
     2581 acaaaacaac tcgagctcac taatgcgaat atatttcgta aaaagccctg tattgttatg
     2641 atacagggtt tttttttgtc ttatattttt ataactcaaa cctaagaagt taattatcaa
     2701 atcaatgaca gcttaatctt ttattagatc gaaatttttt caattaaaag ttagaacatt
     2761 aagtaattgc atggcatttc aaaaaatata ttggcattat cattgagcaa aataagaaat
     2821 gttataaaag cattctactc attagtttcc gtcttctcat tttttaacaa ctaaaaataa
     2881 ttatgaaaaa aaccatcgtt tcaaaaatta cggttttggg gtttgcattt tttttatgca
     2941 atgtgctatc aaccaatgca cagccattca ccgacaactt taacgggcca tcattaactt
     3001 cggcaaatgc ttggacatca tggcaaagca aagagatgtt cggctacact cccaaaacaa
     3061 tatcggccgg accagtattc ggcaaccaat ggttttggtc caatggggtg ggtctaacac
     3121 cagcggctcc tgcaccattc tctgccggag gtatgataaa atttaattct cgtaatggcg
     3181 gattagcccc tgccggtgcc atcgataccg gctgtgctta cattgctaca ccacagctcg
     3241 actattcagg ccgaaatggt gctgctgcaa accttagttt ttatttgtgg cacgaagcat
     3301 atgcaggcgc aacctccgat agcgtggtgg tgtatgcatc aaactcaaac caattacaag
     3361 gagctactcg attaggaaaa gtgtgggccg atcctgcagg ttatggtaat ggctgggttc
     3421 aaagtatatt cgctattcca aacgttgccc aatttaacgg ctgcaacaaa gtgatattta
     3481 tgtttgttgc ttatacttgg accagttata gaaatattta tgttgacaat atctctattg
     3541 ataggtttcc atcaaacatg gttgtacaat ctgcaacatt aaccaatcaa aatacaaata
     3601 gtgttgcatc tggttcaaca aacaatcagg tagttgctgt aaatattaat gtatgcggat
     3661 ccagtactcc caaacaacta gattcaatat tctttaaccc aacaggtaca aatattatag
     3721 cagatattgc aaatgcaaag cttttttaca caggcaacta tcctgtgttt tcaactacgc
     3781 aacaggtttt cgcaactgta aatccaccca tcaataatgt aacatataat aattcactag
     3841 gctttggcaa ttgtgcagcc cccattacat tggctttagg cgataactac ttttggctaa
     3901 catatgacat taagccacaa gcaactgcag gcgatatggt tgatgccgat tttttgaaat
     3961 gcataattca tggtacttgc ttaccgggag gcagtattcc ttttggtgta gcgcaatcgc
     4021 tgcctggcgg tctgttaata ggtgccggtt atattgctcc gttatataca gttggcacta
     4081 gcggcaatgg gtatacttcg ggcgatttta tttcaagtgt gtatgtaaca agcgctgttg
     4141 gcaatcccct tttcaatcaa gaacatgata taacaacttg gttaggttgc accggtatgt
     4201 caggtcagta ttgcaataga catgcacccc accctacaga ctacacctac tttgccccaa
     4261 caaacaatag cattacaaca gataggtcat gttcattaaa cctaggtcaa ggtattcgca
     4321 gcggcactgt tgccgataaa atagctattg ctgcaggcaa ttggttctct tcaaattatg
     4381 tgcgtgcatg gattgatttt aatagtgatg gcgactttga cgacaattat gttgggccgg
     4441 gaggaacaat ttacgaatcg ataatggtga acggtggcta ttccttgagc gataataact
     4501 caatagcatc attaggtaca gtagccggtg cattccccac tctaaaaaaa ggtgacgatt
     4561 ggaacgaatg gaatattgaa gtacccaacg atggtgaatt accaacagtg ggtggaggat
     4621 cgggcgggcc ggcagtgcat accggttatg caaggttgcg cattcgtgaa gtttatgcaa
     4681 atggcacttt tacccctttc agtacacaag ctaatggcga atgcgaagat tatgatataa
     4741 tgttagtcgc cagttgcggt atccctttag taaaaacttg caattggctt ggtggaggca
     4801 taaatcccaa caattggttc gatgctgcaa actggtgccc gggtattcca acacaatacg
     4861 atactgcaat gatacagccc aatgtgaatg ggaactatcc tattattgta tcgaatcaaa
     4921 atccagagtg cgccacctta cgcatttttt cgggagcaca agttacggtt gatgcacccg
     4981 atgttgtaaa tggaaatatt ttagttccca atacaggaaa attaaaggtt taccacaata
     5041 ttgaaattgg agaagcaccc ggcacctttg ctaaattaaa tgttttacaa aatcctgtta
     5101 atagcgttac aacggggata gcatcctcag ctgggacttt attattatca ccatttcgca
     5161 acgataaaca agatgctaaa attcaattta tatatgcccc aaacgagctt gctatcatgg
     5221 gattaaagtc gggcgatgta ttagataaaa ttacctttta cattaaatca gctacagttt
     5281 tgccttcagg aaacttagga aactttacag taaaaatttg gcaatatgat agtacgtact
     5341 cttttccagc attattatca ccagcgaccg aagtggcagt taacaatgcc ttacctggcc
     5401 gaataccatc aaccggagcc ataaatgtta ctacttgcta tggcccttca aattttacag
     5461 ttcccgctat taatcagatt aataccatcg ttcctgttac cattaatttt acggcaaaac
     5521 caagtataaa tgtttcaagg tatttgtgtt tcgaaatatc gcgcgattct ccctttacat
     5581 ccggtgttgc atatcctatt ctttatgaaa gtacattgcc ccgttattgc ttagttttga
     5641 ataatgcaaa caacaatact tcttcaaaca ccattgcagc aaaaattaca ggagttggca
     5701 cagcccctgg ttctgcagga gtaaacgcta acaatggcat attaaataat ggcaactttg
     5761 tagatggtgg agtcagttcc cctttttctt atattacagg atcaacttat gttttccgcc
     5821 ccacatcaga aataggattt acacgccatt actcaaagta caatatagaa gtaggtggta
     5881 actggataaa caataatgct cccggtttgc ctttgggttt cgaagccgga tatagtgcag
     5941 taaaaatgat aaacaattat gtgcccggtt tgcatccaaa taatgatgtt gatacaattg
     6001 gtggaagtga agtaaccata tttagcgaac tcgaaataaa tgatgccaag aatgtaagga
     6061 tgaatgtgat aggtataaat aatggtaaca caggattgat tgttgacagc acattaaaac
     6121 ttaccaccgg acaattacaa ctatcgaatg aaaaagtggt aataaacaat ccaaaccaaa
     6181 atgccattca acgcaccaca ggatttatac agagccagca aacaaataat tacaataaag
     6241 tgcagtggaa tattggcaat aataccggcc cacacacaat accctttggt atagcaataa
     6301 actatttacc gcttacattt aacttagtaa gcggatcgtt tggtaacatt actgcttcta
     6361 cttattacac taatgctgca aatactccac taccggtaac gcccgaagct gttactaact
     6421 tatgctttcc atcgtcaaca aataactctt cacagatggt tgaccgctat tggcaattaa
     6481 atggaactat ccctggtggt ggtgtagcga atatcgaatt tgtatatggc gcaaatgaac
     6541 ttcctccggg cgtgaacgat accgatttaa ttgccacacg atttgaaaat gatacgcttg
     6601 gtgcttgtgc cggaggtacg tggttatcga atgcacaaat gggaaatcct ttgcaacagt
     6661 cggggagtgg ctatgggtat ggtacacatg cagtgcgtat aaataacgtt tctaattttt
     6721 caccatggag cctgacaacc aaatcgagtt ttactcctcc accttgtatt gttacgggat
     6781 tgaaagttac aaacgctacc aacactacag caatgttatc gtgggcaaaa ctcagcagtg
     6841 caacaagcta tagagtgaga atacaaaatc aactttcaaa cttaaatgct tatggtgtta
     6901 ctgtatacac acttgatacg ttcactttgg gaaacaattt tttacccgca tcggcatatc
     6961 gttttcgtgt agctgcaaaa tgcaatggcg tgtggggtgc atggacagct tggaaaaatt
     7021 tcaataccaa cgtaccgaga gaaactaccg atgaaataaa ttttgtagat aatgcaaaac
     7081 catttttatt atacccaaac cctgcaagca ccaacatcac aatagaaaca ggatttgata
     7141 atgactacca aataaaaata atggacacac aaggcaggtt ggtatacagt aacagttata
     7201 atgccgaaaa cgaattgctg aatattgata ttgccaaatt agaaaacggt atttatcaat
     7261 tgcaaataat tacaagcgaa aatatttatt tggaacgagt ggtagtgaat cggtagaaat
     7321 ttttaaatta taataaaagc agtggtgttt ggcttacata catcattgct tttttttgat
     7381 cacaatattg acactttttt ttaatggtta aaaccaggta taagcatcaa atataataaa
     7441 accaaattaa aaccatcatt cgaaaaaaca ttgccacttt tttactcgca gtaaattata
     7501 tttgccgctc caaaactgtt ttgtaattga tgaataagaa aaatatcgat acagcaactt
     7561 tgcattcaat ttttttgcaa tgcaatcaaa agataacaac cgacacacgc aagatagttg
     7621 ccggaggaat tttctttgca ttgaaaggag acaattttaa cggtaatagc tttgcgatag
     7681 aagcagtaaa tgcaggtgct gcatactgcg ttgtcgatga aaatattaat accaacgatg
     7741 aacggatttt atatgttgat gatgtattga aggaattgca aaacctggca actttccaca
     7801 ggcagcacct taatattccg gtaattgcaa tcacaggcag caatggcaaa accaccacca
     7861 aagaattgtt ttatgctgtg ctttctcaaa agtacaacac gcttgctacc aaaggcaacc
     7921 tcaacaatca cataggtgtg ccacttacat tgctatctat tatgccacaa caccaaatgg
     7981 ccattgtaga aatgggcgcc aaccatcaac tcgaaataga acaactatgt aggattgccc
     8041 tgcccgatta tggaatgatt actaatgttg gcaaagcaca cttagagggg tttggtggtt
     8101 ttgccggagt aataaaaggc aaaacagaaa tgtatactca tatcaaaaat acccggggag
     8161 ttattttctg caatgcaaaa aacgaacatt tatatccctt gataaaagat tatgaaaagc
     8221 taatcactta tcttggcgaa gcatcaacaa taaatgcaga atattatgac gaaggagttt
     8281 ttctggcagt agaatggagt aagaaaggcg aaagcaacca taaccatata cgcagccact
     8341 taacgggagc atacaatttc gaaaatattg cggcagcaat tactattgga gattacttcg
     8401 aggttgctgc tcaaaaaata aacgatgcta tcgttaatta tatacccgac aatcaacggt
     8461 cgcaggtaat aactatgccg ggaaacatca atatcattct tgatgcttac aatgccaatc
     8521 ctaccagcat gtaccatgcg ataatgaact tcgataaaaa atttgtaacc aataaagttg
     8581 ctttacttgg cgatatgtat gaattgggtg ccgatgaaga tgccgaacat ttaaaaatta
     8641 ttgaattgat tgcgcaactc aacatacaac aaacagtact tgtaggtaaa cgttttgcaa
     8701 aacacaaaaa caaacttgct gcacattact tcgatacaat tgctaatgca tgtgcgtttt
     8761 taaaaactca gcattttgcc aatcacaata ttttaattaa gggctcgcga agttcgcaaa
     8821 tggacaaagc ggttaacgct atagttgcat agcagaagga ataattattc aacaataatt
     8881 ttttcgttga tggtttcttt accaaaaact attttcaaaa aataaactcc tttaccacgg
     8941 ttttttaaat tgaacgaccg ctcgtacttg cccgtgaatt gcgaagtatt ttccgatgat
     9001 attgtttttc ctgctgcatc ggtaagttgt atattcaaat cgtcaacaat attggtgtta
     9061 aaagtaacat caaaatttcc tttgtttgga ttagggaata tttttagttt ttgaattgca
     9121 ctataatttg ctttgtcctt tgtcggcttt cccttcttga tttcaatccg cttttcattt
     9181 tttattttat ccttatcggc atcaatctta attttaatat ttactttttt atcattcata
     9241 ttttccatgg cctgtttcaa ttcttctttt gccttgcgca tgctattttc tatctcttgt
     9301 tgcgtttgtg catctatacc tttaaaattg aacgacatcg aaaagtcttg accggtggta
     9361 tctattttta tatcgaatga cttgttgagt tcatcttgca gttgttccat atttaaatca
     9421 ttaaaattaa actgatacgt ataggcatca tcatcagtct ccacttctat gtgaatacgt
     9481 tcgagctctt tggccagttc ttcactcatt ttttctattg cccggcccga ctcgtgcaaa
     9541 ctttcttcca tcaatcttaa agcatcttcc atttctttgc catcaatatt aatatctacg
     9601 ttgctgtttc ttctatcggg tgcacccaca ccatgttgct tcataaattc atcggcagcc
     9661 gctttgtcat tttcttcaaa tacagtgtca attaaaattt gcttgccatc aatatctttc
     9721 aatattttga catagatttt tccatcgcct ttagatgtat tttgtgcatt tgcagaataa
     9781 attccagcaa ataaaaataa agcaaaaatg ctgagtatag aatttttcat aacgtatgtt
     9841 tttgaattag aggcaaaagt atacacaaaa gttacaaggc agagttaagg ccgtattaat
     9901 gccgaggtta attatacgtt aaagcaaaac aggccaaatg ttaaaatgta tttttacatc
     9961 gttgttaata gggaactaaa agaaaataga tacaagcgta tcgaaaaagt taaatgaaca
    10021 aaacgcattt caaaataatc atatcggtaa tgagtatagc attactgggt ttggtagtgc
    10081 tgcaagcgta ctggataaaa cacgatttcg atttgaagga aaagcaattt gaccaaatgg
    10141 ttaccagttc gctcgatgcc acagtgaaca aactcgatat ggaggagacg tttaaaatga
    10201 tgtcgcaggc tgggctaata aatgcaaata gttttgccgg catgatagat tcatcgttgg
    10261 catggggtgt aacatacgaa gatgaaaacg acagtgtggc actgaaggca acggatacca
    10321 tgggcgcaaa gcaacggtac ggtgaaggca ctcaaatggt tatggaagaa gttaaagatg
    10381 gaaagaaaat aaaaaaaata attcaggctt acgatagcac atttatatcg aaagaccaat
    10441 cattgaaaat aaatgataag cgcatacaaa gcatcataca aactgaaccg gtgttggcaa
    10501 atgttgaaga ccaatataaa t
//
LOCUS       DAORQA010000048        10483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1704_length_10483_cov_12.115650, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000048 DAORQA010000000
VERSION     DAORQA010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10483
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1704_length_10483_cov_12.115650"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /gene="galE"
                     /locus_tag="PK736_04525"
     CDS             <1..1002
                     /gene="galE"
                     /locus_tag="PK736_04525"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRC32687.1"
                     /translation="VTGGTGFIGSHTTVELIKKGHTVFIIDNLCNSRIEVIESIKTIT
                     GTKPQFSQFDICDSNACERFFSENKIDAIIHFAALKAVGESVAKPLYYYQNNLQSLFN
                     LLAMCNKFSVQHFVFSSSCTVYGEPDVLPISESAPVKQPESPYGNTKKISEDILRDCS
                     KAYDINVIALRYFNPVGAHDSALIGEYPLGKPNNLMPIITQSAIGKLDGFSVFGTDYD
                     TPDGSCIRDYIHVVDLAHAHVIAIERMANKQNKSKYEVFNIGTGVGISVLQMVNAFEK
                     ITGIKLNYKLDNRRPGDVVKVFADTNLANAELGWKSTLGLDEMISSSWAWEQKLATK"
     gene            1310..2860
                     /locus_tag="PK736_04530"
     CDS             1310..2860
                     /locus_tag="PK736_04530"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HRC32688.1"
                     /translation="MALDKDIFISYAHLDNKELPGEKEGWVSDFHKALEVMVGEYLGK
                     APVIWRDSKLQGNDFFAPEIVEKFRELKVMVSVISPRYLTSEWCTKEVDGFYDAAQTT
                     GGISIENKARIFKIIKTPVNFEELPERIKPILAYEFYSTDSASGTTRTFNKIFGKENE
                     VAFWTKMDEVAQHLAQLIKKFNTGSVPIKVVETSAAIVNEKKFTNRTIYLADTTNDVK
                     EFRENIKRELEDHGFKILPDKNLTLEDSLYKKEVADYLKQSTMSLHIIGSKYGMIPED
                     SEKSILVLQNDIAAAQSTDSGLSRLIWVPPTDKPAEERLQPFIDHLKQKEDLQNLSDL
                     LVGSLEEFKFAIFDTIKKLDARDKAAEEAKQKAKEVTPEKVVATPTVSTGPKVIYLVC
                     DKRDLDNTKELEDFLFDCKFDVMLPVFDDDETESRKINDEYLALCDAAVLYYGSANEA
                     WLRSKSSELQRKAALENSKELLAKLVYIAGPSTPQKERFRSHDLMVANGLTGLNKDDF
                     KAFTDKLK"
     gene            2873..5695
                     /locus_tag="PK736_04535"
     CDS             2873..5695
                     /locus_tag="PK736_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRC32689.1"
                     /translation="MALKSTKLKNPFVGLRSFEEEEDYLFFGRSAEINDLLKKFASSP
                     FLAVVGSSGSGKSSLVKSGFLPSIYSGFLSAGRNWRVALMRPGDNPIGNLAQSLAVDS
                     VLYDTNNNPDIPRSSIIEAVLRRSGNGLINVYDRANLTETDNLLIIVDQFEELFRFSR
                     YEKRIHEERSDALNFVNLLLTASAQRVAPIYVLITMRSDFLGDCSQFRGLPEAINNGQ
                     YLVPRMTRDEIREAITGPIKVGGAEITPRLLTKLLNDLGTNTDQLPILQHALMRTWDE
                     WIKRNEPQSPIDIEDYEKIGSLAEALSLHADEAYKELDTDEKRKICELMFKALCDTNA
                     DVRGIRRPQLVSELADITQAPMPKVIEVVDVFRKEGRTFLMPPATVTNLTNNSIIDIS
                     HESIMRKWQRLIQWNAEEAKSGEIFVRLADEAKLYDAKEVSLYKDGEPELAIATKWYK
                     DNKPNAAWALRYNTNYESAIGFLEKSEQNAKEERKEKERIARNKKRFAFGVIVFFALL
                     ALAAGFVSYYMFGLKNEAETAKSKSDSTATYADSVRIVSDTNAEKAIRAESAALDSAE
                     AAKIARNDAMIQKAYAEQLTLKAQLLNKQLETEQATLKKAYEDLEKQSRKIAASAYKD
                     SIREGPTIESEIKKVNFDLTLVAYIYHLVDSLGVEAKSSELYPKVFACLSANSNKDSV
                     LSKQKEVQNFTDSISLGNDTKISLSKDKLKVMLNLKGYNVDSINVSKNPVECFAADNV
                     DSLIFCGTDSNTIKIYKYNEKFKLTKEVTVPLNGLVTAIDYNAVNDVIYFGLRTGEIG
                     YIQYKNKKYDNLKTVYENELGSKVTAIDFFEHNKKFYLLATSLKGKVIVYDFGPADPV
                     SALTYLVPDKKLTGIELPENLREIQHARFDELSGKVKLASAKGNYDWNPFTDEALKML
                     ENWINKDIDRQKELERITKDKKTKWY"
     gene            5701..9939
                     /locus_tag="PK736_04540"
     CDS             5701..9939
                     /locus_tag="PK736_04540"
                     /inference="COORDINATES: protein motif:HMM:NF040284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32690.1"
                     /translation="MKKIFQIILAIILFNSMNGLMAQCDITVTPASITICAGDSVTLF
                     ATDTGNLAQFKWTPAINLSATNGSTVIAYPTTTTIYTVKKNGCPDSATTQVIVKPQLF
                     VLASGDDSICDGETLNLFATVFGGNGIYLYDWNGPDGFSSSLKNPTLANVTNLSAGTY
                     TVTVTSGGCSQTDSINVTVKELPFANAKADTSFCNGTLVNPIIFTSNPPGSTFTWTSS
                     QNLNFGIAGSGNIPPFIATNTTQFNRVATVTVTPTFKGCVGQSMQFIITIKPTPAFTS
                     ATEAHICNGNVFTYTATAKPVLNTSFAWVKAADGFGNGSSSATGATINETLFNSSNLP
                     IEVYYSFTLTRPGNTCPYIDTLTVTVYPTPKINPTYDSTFCNGEPGYPILFTSQSPET
                     AIEWSSDKDVGFGLSGYSGIQGFVATNNTSDPIVATVTVKIKASEDSCAGPDSTFTIT
                     VNSAPRLNSTLVSTVCNNDSLIYTATSPTPNLDFSWSRPAVSGINGGTAATGNDSTIK
                     DQLVNTDTVPIPVWYIFNMTTSTGSCAGDDSILVTVFPTPKLSSDTSSVQLCSGDLFV
                     YNATCATPGTSFYWSRDSVTGILPDTANGSSNLIQETLINTTDSIITVKYIIGLSANG
                     CDTSTQSITVTVNPTPRLSNTVFNFTTCNDVFFNFNATSNTEGTPTFTWTRDSVFGIT
                     PYTSTGTNATIIDSLHNSTPNPIIVTYAITIVANNCTSIVNVTDTVFPTPVLTDTILK
                     DTLCSGNTFVYNASSLTAGVSYNWSRAAVAGINNNNPSSGSTALIQEALINNTNTVQT
                     VTYQISLSAYNCPANIQSLTVTVNPTPVLSNTVLLKDSVCSGDVFSRTLSSNVPNTNY
                     QWVRDSVPGITPSNGNGNTNTITETLINAEPYPIDVIYNIKLTTSDSCTNTQNIVVTV
                     YPKPVLLDNEKSDTICSGSVYTYLGLSATVNTTLTWQRNTITGILPLTANGTSNSIVD
                     SIINTTSSPIIVKYIFSLTSAFGCVGTDSIELLVNPTPFIYNSSLSVCSGTPFQYQIV
                     PAYATSSIVYSWTTDSTVNITGNYPQLPENANSLIEDTLNNSLNNAIVPVKYNIMATF
                     IDTNVRCSSMASLTVNVLPLPKLPLFTSLSANANTIDLCGGSANINFNILPASIQQGV
                     SYVWSANPNTNVFIGDTTNPNTVISFNNFNGTAQIVVTANNIDSLGKCSVNESQQVVL
                     SIDNDSIQERNIFLKQPGHFLIYPDNAMDPSSDTNGIFNGYQWGYDTIANIAKPDSLG
                     APREISGQVYQFFIPDKKYINLQGLDTLNYAWWVLLRHGECRSKVYYNGPFAYGRYLE
                     QPNNDGIVYVNVFPNPGTGNFMIMLKGKMYGKINAHVYDGMGREVFFNNFEKKTYELN
                     TPLELSSLPNGIYLLELQSEDLQKVSTRIFIIHN"
     gene            9948..>10483
                     /locus_tag="PK736_04545"
     CDS             9948..>10483
                     /locus_tag="PK736_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32691.1"
                     /translation="MKKLILYFILLGLPMFSMAQSQLEKTDSLNTYIINELQDYWNLG
                     IDSAGKELPNFRTGLNHARLFTKDAIIDDDINAVFRPEKVEDQNPYKTGTAKAEEWYA
                     HDLTLQFKSLKVDTVYNIKYNSGSGDINSNKSSVEFTRKISGQKNRKYVFDDPASLLN
                     TLKTNKNNKQSISDFNDS"
BASE COUNT         3409 a         2074 c         2046 g         2954 t
ORIGIN      
        1 gtaacggggg gcacagggtt cattggttcg catacaacgg ttgaactaat aaaaaagggt
       61 catacagttt ttattattga taacttatgc aattcgcgca ttgaagtaat agaatctata
      121 aaaacaatta ccggaacaaa accacaattt tcgcagttcg atatttgcga tagtaatgcg
      181 tgcgaacgtt tttttagtga aaataaaata gatgctatta tacattttgc agcattaaaa
      241 gccgttggcg aatcggtagc caaacctttg tattattatc aaaacaattt gcaatcgctt
      301 ttcaatttgt tggccatgtg taacaaattt agtgtgcaac attttgtttt ctcctcatcg
      361 tgcacggtct atggtgagcc cgatgtattg ccaataagcg aaagcgcgcc tgtcaagcaa
      421 cccgaatcac cctatggaaa tacaaaaaaa ataagcgaag atattttgcg cgattgctcc
      481 aaagcatatg atatcaatgt tattgcactg cgatatttca atcctgttgg cgcacatgat
      541 tctgccctga ttggagaata tcccttaggc aaacccaata acctgatgcc catcattact
      601 caaagtgcaa taggcaagct tgatggcttt agtgtgtttg gtactgatta cgatacaccc
      661 gatggcagtt gcattcgcga ttatatacac gttgttgacc ttgcacatgc acatgttata
      721 gctattgagc ggatggccaa taagcaaaat aaaagcaagt acgaggtatt taatattggc
      781 accggtgtag gcataagtgt attgcaaatg gtaaacgctt ttgaaaaaat aacaggaata
      841 aaactcaact ataaactcga caaccgtagg cctggcgatg tggttaaagt ttttgccgat
      901 accaatttag ccaatgccga actgggatgg aaatcgaccc ttggtcttga tgaaatgatt
      961 tcttcatcgt gggcctggga acaaaaactg gcaacaaagt agtttttttc agtgcccatt
     1021 cgttcagcca ggattttaaa ttttgttata ctaacaatct taaccttttc atcccataaa
     1081 tttgtccatt agccaaataa gccttaatag gtgtgttaat aacggatgat aaatatatat
     1141 ttgtagtgta attgttttaa cagtataatt agtcgttaga catacctcaa aaaaatcact
     1201 cgaatctatt ttcatatttg ctttttgggg tttctaaata actattcacg tgtgtaaaaa
     1261 agttactaac ctagctgttt agattcttca atttctaaaa tcataaaata tggcacttga
     1321 taaagacatc tttattagct atgctcacct cgacaacaaa gaattgccgg gagaaaagga
     1381 agggtgggta agcgattttc ataaggcttt ggaagttatg gtaggtgaat atcttggaaa
     1441 ggcacctgtt atatggcgcg attccaaatt gcagggtaac gatttttttg cccccgaaat
     1501 cgttgaaaag tttcgcgaac taaaagtaat ggtttctgtt atttcccctc gttatttaac
     1561 ctccgaatgg tgcacaaaag aggtggatgg gttttatgat gctgcacaaa ctacaggtgg
     1621 cataagcatc gaaaataaag caaggatttt taaaattatt aaaactcctg taaactttga
     1681 agaactgccc gaacgcataa agcctatttt agcatatgaa ttttatagca ccgattctgc
     1741 aagtggtacc acccgtacat tcaacaaaat ttttggtaaa gaaaacgaag tggccttttg
     1801 gaccaaaatg gatgaagtag cacaacatct tgcccagcta attaaaaaat ttaacaccgg
     1861 ctcagtacca attaaagtgg ttgaaacaag tgccgccatt gtaaacgaaa aaaaatttac
     1921 taaccgaacc atttaccttg ccgatacaac taatgatgta aaagaatttc gcgaaaacat
     1981 aaagcgcgaa cttgaagatc atggattcaa aatattaccc gataaaaacc tcactctcga
     2041 agatagttta tataaaaaag aggttgcaga ttacctaaag caatcgacaa tgtcgttgca
     2101 tattataggg tcaaagtatg gaatgattcc cgaagactcc gagaaatcta ttctggtgct
     2161 tcaaaatgac atagccgctg ctcaaagtac agacagcgga cttagccggc ttatatgggt
     2221 gccccctact gataaacctg cggaagaacg gctgcagcct tttatcgatc atcttaaaca
     2281 aaaggaggac ctgcaaaacc tttctgattt gctggtagga tctctcgaag aatttaagtt
     2341 tgcaatattc gataccataa aaaaacttga tgcccgcgat aaagctgcag aagaagcaaa
     2401 acaaaaagct aaagaagtta ccccagaaaa agtggtggca acacccacgg tttcaacagg
     2461 gcccaaggtt atatatcttg tttgcgataa gcgcgattta gataatacaa aggagctcga
     2521 agatttttta tttgattgta agttcgatgt tatgttaccc gtatttgatg atgacgaaac
     2581 cgaatcgcga aaaataaatg acgaatacct cgcgctttgt gatgctgcag ttttgtatta
     2641 cggtagtgcc aacgaagctt ggctgcgtag taagtcgagt gagctgcaac gaaaagccgc
     2701 cctcgaaaac agcaaagaat tgttggcaaa gctcgtttac attgccgggc catcaacacc
     2761 tcagaaagaa cgattccgct ctcacgactt gatggtagca aacggcctga cagggttgaa
     2821 taaagatgac ttcaaagctt ttaccgacaa actaaaataa aagaaccact gtatggcgct
     2881 taaatctact aaactaaaaa atccttttgt cgggctacga tcttttgaag aagaggaaga
     2941 ttatttattc tttggccgga gtgccgaaat aaatgacttg cttaaaaaat ttgccagcag
     3001 ccctttcctt gcagttgtgg gatcatcggg tagtggaaaa tcatccttag taaagtcagg
     3061 atttttgcct tccatatata gtggattttt atctgcaggg cgtaattggc gtgtagcgct
     3121 tatgcgaccg ggcgataatc ctattggaaa tcttgcccaa tctctggctg tcgactctgt
     3181 gttatatgat acaaacaaca accccgacat cccccgcagt tcaattatag aagcagtgct
     3241 aagacgaagc ggcaacggac tgataaatgt atacgatcgt gcaaacctta ccgaaaccga
     3301 taacctctta attatagttg atcaatttga ggaactcttt cgttttagcc gatacgaaaa
     3361 acggatacat gaggagcgca gcgatgcatt aaattttgta aatcttttgc ttactgcttc
     3421 ggcacaaagg gtggcaccta tttatgtgct tatcactatg cgttccgatt ttctgggcga
     3481 ttgttcacag tttcgtgggt tgcccgaggc tatcaataac gggcaatatc tggtaccacg
     3541 catgacacgc gatgaaattc gcgaagccat taccggacca ataaaagtgg gcggtgccga
     3601 aataacacca aggctactta ccaaactact taacgacctt ggcaccaata ccgatcagtt
     3661 gcctatactg caacatgcac ttatgcgcac ttgggatgaa tggataaaac gcaatgaacc
     3721 acagtcgcca atcgatattg aggattacga aaaaatagga tcgctggcag aagcgctttc
     3781 gctgcatgcc gatgaagcat acaaagaact tgataccgat gagaagcgca aaatttgcga
     3841 actcatgttt aaagcccttt gtgataccaa tgccgatgtg cgcggtatac gcaggccgca
     3901 attggtttcc gaactcgctg atataactca agcgccaatg cccaaagtta ttgaggtagt
     3961 ggatgttttc agaaaagaag gccgcacttt tcttatgccc cctgctactg taaccaacct
     4021 taccaataat tcgattattg atatatcgca cgaaagcata atgcgcaaat ggcagcgcct
     4081 catacaatgg aatgctgaag aggccaagag tggagaaata tttgtgaggc tggccgatga
     4141 agcaaaatta tatgatgcaa aagaagtgtc gctgtataag gacggtgagc ccgaacttgc
     4201 cattgccacc aagtggtaca aagataacaa acccaatgcg gcatgggcac taaggtataa
     4261 taccaattac gaaagcgcta taggtttttt ggagaaaagc gagcagaatg caaaagagga
     4321 aagaaaagag aaagaacgca tagcacgaaa taaaaaaaga ttcgcctttg gcgtaattgt
     4381 tttttttgca ttgctggcat tggcagcagg gtttgtttct tactatatgt ttggtttaaa
     4441 gaatgaagca gagacggcta agtcgaaatc cgatagtaca gctacatatg cagattcagt
     4501 gcgaatagta tccgatacca atgcagaaaa ggcaattcgt gctgaatcag ccgcactcga
     4561 ttctgctgaa gctgctaaaa tagcaaggaa cgatgccatg attcaaaaag catatgctga
     4621 gcaattaacc ttgaaagcac agcttttgaa caagcagcta gagacggaac aagctacgct
     4681 taaaaaagcg tacgaagatt tagaaaaaca aagccgtaaa attgctgctt cggcatacaa
     4741 ggattctata agagagggcc caaccattga atccgaaatc aaaaaagtta atttcgattt
     4801 aacccttgta gcatatattt atcatcttgt tgattcattg ggtgtggagg ccaaaagcag
     4861 cgaactatac cccaaagtat tcgcatgcct ctctgctaat tcaaataagg attcggtatt
     4921 atcaaaacaa aaagaggtac aaaattttac tgatagtatt tcgcttggca acgatacaaa
     4981 gatatcgctc agcaaagaca aattaaaagt aatgctaaat ttaaaaggtt ataatgtcga
     5041 ttcgattaac gtgagtaaaa acccagttga atgttttgct gccgataatg ttgatagcct
     5101 tattttttgc ggtacggata gtaacacaat caagatatat aaatacaacg aaaaatttaa
     5161 attgaccaaa gaagtaacag tgccattgaa tggtttggta acggccatag attataatgc
     5221 ggtgaatgat gttatttatt ttggattgcg cacaggtgaa ataggatata ttcaatacaa
     5281 aaataaaaag tatgataatc ttaaaacggt ttatgaaaat gaactgggat cgaaagtaac
     5341 agccattgat ttttttgagc acaacaaaaa gttttatcta ttggcaacaa gcctaaaagg
     5401 aaaagttatt gtttatgatt ttggtcctgc agatcccgtt agtgctttaa cttatctggt
     5461 acccgataaa aaactaacag gaattgaatt gcccgaaaat ctcagagaaa tacaacatgc
     5521 ccgtttcgat gaattatctg gcaaagtgaa gttagcatcg gcaaaaggca attacgattg
     5581 gaatcctttt actgatgaag cgcttaaaat gctggagaat tggataaata aagatatcga
     5641 ccggcaaaaa gaattagaaa gaataacaaa agataaaaaa accaaatggt actaaataca
     5701 atgaaaaaaa tatttcagat tattcttgcc atcattttgt tcaatagcat gaatggatta
     5761 atggcacaat gcgatattac tgtaactccg gcaagcatta ccatttgcgc aggcgatagt
     5821 gttacccttt ttgctaccga tacaggaaat ctggcacagt ttaaatggac tcctgccata
     5881 aaccttagtg ccaccaatgg aagcacggta attgcctacc ccactactac cactatatac
     5941 actgtaaaga aaaacggctg ccccgatagt gcaacaactc aggtaatagt taagccacaa
     6001 ctttttgtat tggcttcggg cgatgacagc atttgtgatg gcgaaacatt gaacttattt
     6061 gcaacagttt ttggaggaaa tggcatttat ttgtatgact ggaatggccc cgatggattc
     6121 tcgtcttcat tgaaaaaccc cactttagca aacgttacaa acttatctgc aggtacgtat
     6181 acagtcactg ttaccagcgg tggttgctct caaacagatt caattaatgt gactgtgaag
     6241 gaattacctt ttgctaatgc caaagctgac acttcttttt gcaatggtac tttggtaaat
     6301 ccaattatat ttacaagcaa tcctcccggt tctaccttca catggacgag ctcacaaaac
     6361 ctaaattttg gtatagcggg tagtggcaat attcctccat ttattgctac aaatacaaca
     6421 caattcaaca gagtggcaac cgttactgta acacccacat ttaaaggttg tgttgggcaa
     6481 tctatgcaat tcataattac tataaaacca acacctgcat ttaccagtgc tactgaggca
     6541 catatttgca atggcaacgt tttcacttat acggctacgg caaaacctgt gctaaacact
     6601 tccttcgctt gggttaaggc tgcagatgga tttggaaatg gcagtagctc tgctactggt
     6661 gctacaataa atgagacttt attcaatagc tccaacttgc caatagaggt ttattattct
     6721 tttacattaa cccgaccagg caatacatgt ccatacattg atacattaac agttaccgta
     6781 taccccactc cgaaaataaa tcctacttac gattccactt tttgtaacgg tgaacccggg
     6841 tatcccattt tattcacaag ccaatcgccc gaaacggcta tagagtggtc gagtgacaaa
     6901 gatgtcggct ttggcttgtc ggggtatagc ggcattcagg gttttgttgc caccaacaat
     6961 acaagcgatc ctattgtagc cacagtaaca gttaaaataa aggccagtga agattcttgc
     7021 gcagggcccg acagtacttt caccattact gttaattcgg caccacgtct taactccact
     7081 ttagtttcaa cagtgtgcaa taatgactct ttaatataca ctgccacaag ccccacccca
     7141 aacttagatt ttagttggag caggccggca gtgtctggta taaatggtgg cacagcagca
     7201 accggaaacg attcgaccat taaagatcag cttgtaaaca ccgacactgt ccctataccg
     7261 gtttggtata tttttaatat gactacaagt acgggatcat gtgcaggtga tgattctata
     7321 ttagtaactg tattcccaac cccaaaactt tcgtccgata caagttcagt gcaattgtgc
     7381 agtggcgatt tgtttgtata caatgctaca tgtgccacac cgggcacttc attttactgg
     7441 agccgcgatt ctgtgactgg cattttacca gatactgcaa atggtagttc caatcttata
     7501 caagaaacct tgattaacac taccgatagt ataataactg taaaatacat aataggattg
     7561 agcgcaaacg gatgcgatac aagtacacaa agcataacgg tgactgtaaa ccctacgcca
     7621 cgactatcga acaccgtttt taattttaca acctgcaatg acgttttttt taattttaat
     7681 gccacaagca atacagaagg gactcccact ttcacttgga caagagattc ggtatttgga
     7741 ataactccgt atacaagtac cggtacaaat gcaactatta tagattcgct tcacaacagt
     7801 acgcccaatc ccataatagt aacttatgct attacaattg tggctaacaa ttgcacatca
     7861 atagtaaatg taaccgacac ggtttttcca acaccggtct taaccgacac tattcttaaa
     7921 gatactttat gcagtggaaa cacatttgtt tacaatgcaa gtagtttaac tgcaggggta
     7981 agttataatt ggtcgcgagc cgctgtggcc ggaataaata ataataatcc ttcatcggga
     8041 agtacagctt taattcaaga ggcgcttatt aacaatacaa atacggtaca aactgtaaca
     8101 taccaaataa gcttatctgc ttacaattgt ccggcaaata ttcaaagcct aacggtgact
     8161 gtaaacccta ctccggtttt atcaaatacg gtattgctta aagacagtgt gtgcagtgga
     8221 gatgtatttt cgcgaacctt atcttctaat gtaccgaata ctaattacca atgggtaagg
     8281 gattcggtgc cgggaattac accttctaac ggaaatggaa acacaaatac tattaccgaa
     8341 acactcatta atgctgaacc atatcccata gatgtaatct ataatattaa actcactaca
     8401 tcagacagtt gcaccaatac tcaaaatatt gttgtaacgg tttaccccaa acctgtttta
     8461 cttgataatg aaaagagcga caccatttgc agtggctccg tgtatactta cctgggttta
     8521 agtgctacag tcaacacaac attaacatgg caacgaaata ctattaccgg tatactacca
     8581 cttactgcca atggcacttc caattcaata gttgattcca ttatcaatac cacatcatct
     8641 cccattattg taaaatatat attttcattg acttctgcct ttggatgcgt tggtacggat
     8701 tccattgaac ttttggtgaa tcctactccg tttatttata attcaagtct atccgtttgc
     8761 agtggcactc cttttcaata tcaaatagtt cctgcctatg ctaccagtag tattgtgtat
     8821 tcatggacta cagacagcac ggttaacatt accggcaact atccgcaact gcccgaaaac
     8881 gccaattcgc ttatcgaaga taccctcaac aacagcctta acaatgctat tgttcctgta
     8941 aagtataata ttatggccac atttattgat accaatgtac gttgttcttc aatggcaagc
     9001 ctcacagtga acgtgttgcc attaccaaag ttaccgctat tcacttcttt atctgctaat
     9061 gcaaacacca ttgatttgtg cggaggttct gccaacatca atttcaatat tcttcctgcg
     9121 tccattcaac agggagtatc gtatgtatgg tcggccaatc caaatacaaa tgttttcatt
     9181 ggcgacacca caaaccccaa cacggttatt tcctttaata attttaatgg tacagcacaa
     9241 attgttgtta ctgccaacaa tattgattcg cttggcaagt gcagcgtaaa cgaaagtcag
     9301 caagtggtgt taagtattga taatgattcg atacaggagc gaaatatatt tttgaaacaa
     9361 ccggggcact ttcttattta tcccgataac gccatggacc ctagtagcga taccaatgga
     9421 attttcaatg gttatcagtg gggttacgat accatagcaa atatcgccaa acccgatagc
     9481 ctgggagctc cccgcgaaat atccggacag gtttatcaat tttttatacc cgacaaaaaa
     9541 tatatcaact tacaaggcct cgacacttta aattatgcct ggtgggtatt gttgcgccat
     9601 ggtgaatgcc gtagcaaagt atattataat ggcccgtttg catatgggcg ctatttggag
     9661 caaccaaaca atgatggtat tgtttacgtc aatgtttttc ccaatccggg cactggcaac
     9721 tttatgatta tgcttaaagg taaaatgtat ggaaaaataa atgcacatgt gtatgatgga
     9781 atgggtcgcg aagttttctt caataatttt gaaaagaaaa catacgagtt gaatactcct
     9841 cttgagttga gtagtttgcc taatggaatt tacttgcttg aattacaaag cgaagatctt
     9901 caaaaagttt caacacgaat ttttataatt cataattgat ggctaccatg aaaaaattaa
     9961 ttttatattt tattcttttg ggtttgccaa tgttttcaat ggcacaatca caattggaga
    10021 aaaccgattc gctcaacacc tatattataa atgagttgca ggactattgg aatttgggta
    10081 tagattctgc cggcaaagag ttgcccaatt ttagaacagg attaaatcac gcacgcttat
    10141 ttacaaagga tgccattatc gatgatgata taaatgccgt atttcgtccc gaaaaagttg
    10201 aagaccaaaa tccatataaa acaggtacag ctaaagccga agaatggtat gcgcacgatt
    10261 tgactttgca attcaaaagc ctgaaagttg atactgttta caacataaaa tataatagtg
    10321 gaagtggcga tatcaattct aataaatcat cggtggaatt tacacgcaaa atttcgggac
    10381 aaaaaaatcg caagtatgta tttgatgacc ctgcttcatt attgaacacc ttaaagacaa
    10441 ataaaaacaa taaacagagt atttcggatt ttaatgatag tat
//
LOCUS       DAORQA010000049        10006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1788_length_10006_cov_18.825344, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000049 DAORQA010000000
VERSION     DAORQA010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10006
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1788_length_10006_cov_18.825344"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PK736_04550"
     CDS             <1..285
                     /locus_tag="PK736_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32692.1"
                     /translation="LRQKAKRKIATASMYPNPADEKVTFNYYSNASQKLKIELYNSFG
                     QKVYDNNLSNTSTAFDLSLKLYAPGIYCCKILSDGQSILSEKLVIIHAHE"
     gene            278..>541
                     /locus_tag="PK736_04555"
     CDS             278..>541
                     /locus_tag="PK736_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32693.1"
                     /translation="MNNIIKSLFTLLIVCSLNCFSQQGINNLWIEGYGNDSVVLPFGN
                     TDIDFFNGNPTITYNIRGQHLAHTHCNISDSLGNLKFYTNGYDI"
     assembly_gap    542..560
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <561..1801
                     /locus_tag="PK736_04560"
     CDS             <561..1801
                     /locus_tag="PK736_04560"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32694.1"
                     /translation="DASDSVMLNGDSISPSSKQNVFPYGLDVPQANLVIPNPTENNKY
                     YLFHSTNDAWAYRYCLNLMYSEIDMTLNNGLGAVTANKNVILINDSLNLGKIAACKHG
                     NGRDWWVICMRSYSNIFYKLLVTPYGISAPDTQAIGQIRKQGWGELKFSSGGTKLGYA
                     QADYVTNGHIELFDFDRCSGLLSNAQHIIIPQGLSFGGGIEFSPNESLAYVTNADSIY
                     QFDLYSTDISNSRIIVSVSDGYIDTIMNTNFLVGFMNTKLAPDGKIYITSGNSTHYMS
                     VIDQPDSPGLACNVMQHAVQLPALYFNTLPNHPNYFLGANGMCNNLGLPIPSKGGAKK
                     IQVFGNPTHDKLTLWFPVDKDVGWLEIYDVNGECIRRERVSQWSQYKTVDISLFAEGV
                     YFCRMVWPDGEGSVKVVRLE"
     gene            complement(2076..2567)
                     /locus_tag="PK736_04565"
     CDS             complement(2076..2567)
                     /locus_tag="PK736_04565"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HRC32695.1"
                     /translation="MCKLIFLLILAAMFQSCAPSTTIFFVRHCEKESAMPNADLNAQG
                     RLRAQQLAHTLQNVKFAKIYTTNVPRAASTAEPLRLLNNIDSVVYNYHDTVYMAKVLA
                     EDYEAKNVMVVGHSDNIPAMIRNLGGTMPYHEIPLDEFDNLFVVVVQKRQPTKVMHLK
                     YGE"
     gene            2668..4503
                     /locus_tag="PK736_04570"
     CDS             2668..4503
                     /locus_tag="PK736_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32696.1"
                     /translation="MRKKLLILSLFCLALFSLKAQTNGGPDAYGYTWRDSNDPNGQPY
                     NWIDITNLPFVNSVKLLADDNVVGPFPMGFNFHYYWYDVDEFRIGSNGYISFNNGNMA
                     VPFPPIPQPSKLNNYIAAFGSDLSFADAGNPGECYFWKSPGNDTLIVSYINVPFWDAS
                     LLYDGSNTFQIILSALDSSITFQYQDQQGVYNGLFADFLTVGIENNSGNIGLMHSHDI
                     YPVASYSIKFYPPSTVTYTAIDATCDWVDNVSSGGRFLTKNGTPYEMKSNIRNYGNTV
                     LAPFSVLSEIKSGFSQVVAGTATTDTLIPGQSQILTYPNNTWNHNTTGTFSMITNTQL
                     ANDLVPSNNKDTCEFVVIDSTTASQWLSFDDGSVGGVGLSWNGGGPGEGVGMQFPLPY
                     FPIQIDEIRALVVANPTPEGMALKIIDDDGVGGTPLTQLDSVWVDPFSVQVGTYTTVQ
                     LTNPIIKTSGSVYVMWEMGGNGISIGQNVTRPISNRSFEVLGGSWSIYRSREDEDFII
                     NIKVSDPTVGIAEQNPLATVFGNMFPNPAQNFVNINYNTSTIINNITLEICDMQGRIV
                     QTKNIASLYGEGSMKVSIAELSAGMYICKMSVNDKSINKRFTIVR"
     gene            4654..5526
                     /locus_tag="PK736_04575"
     CDS             4654..5526
                     /locus_tag="PK736_04575"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HRC32697.1"
                     /translation="MELPKGKLISIGGAEDKGTEAEPNFTLKNNLNFFELQILQRIVL
                     ETGGGEKHIEVITTASSIPEEVGEGYLKAFGKLGCKNVQIMHIKKREDTQNPEFIERI
                     KTCDTVMLTGGNQLRLSTIIGGTEIHKILMHRYMEEEKMLIAGTSAGAMAMSNTMIYQ
                     GSSSEALLKGEVKLTTGLGLIKDVIIDTHFVKRGRFGRLAEAVASNPSCIGIGLGEDT
                     GLLITEGTLMEAIGSGLVIIIDGHNIKYTNIADVQYGSPISIENLVVHVMAKGNHFDL
                     KQHRFFEDVINVSK"
     gene            complement(5541..6836)
                     /locus_tag="PK736_04580"
     CDS             complement(5541..6836)
                     /locus_tag="PK736_04580"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRC32698.1"
                     /translation="MSSFNSTLEYLFAQLPMYHRIGEAAYKANLDNTQRIMEVLQHPH
                     QNFLTVHIAGTNGKGSVSHSLASILQQAGYKTGLYTSPHLKDFRERIRIDGKMIDKKY
                     VTDFVQKNKQELETIKPSFFEWTVGLAFQYFKDKNVDIAIIETGLGGRLDSTNVITPV
                     LSVITNISLDHINLLGNTVAKIAREKAGIIKPGVPVVIGEHDEATAKIFEQFARKNKA
                     PIVFASQNFTVRKSITKIKSGTTLKLDVNKGKKKLYEKLQLDLTGDYQLKNVCTVLQS
                     VEVLQEFFKIKPRDIYAGLKSVSASTGLQGRWQVIAGKPMTVCDTAHNVAGIVMILKQ
                     IEPNKFKQLHIVIGMVNDKDIASILKIMPPNATYYFCKPNIPRGLDAEQLLIKAQAFN
                     LKGKAYRTVKLAYNAARKSASQQDFIFIGGSTFVVAEVI"
     gene            complement(7104..7991)
                     /locus_tag="PK736_04585"
     CDS             complement(7104..7991)
                     /locus_tag="PK736_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32699.1"
                     /translation="MEAQIRTRTYISTAIVYGILILLLFILKLYTEIPPFGGGGGGGG
                     NLGYVELASGNIQPMSEETTTTPNPVEETAKPTPATPEEIMTQENEESAKIEEIPKPK
                     KETEKPVVKKIEPVKTVVPEKVVVKKEPERKPDPNALYPGKSNNSTSQGTANTGKGDQ
                     GTPGGNPFGGYGKGDGSGGGNGGGKGDGIGDGTGNGTGPGGGGNGMKVNLTNREIRSR
                     PQVSDNSQETGTVVVDITVDKNGNVVIASPGGQGSTTTSKHLYELSKLKAKEYKFNAS
                     PDGSELQKGTITFVYKYKK"
     gene            complement(8052..8456)
                     /locus_tag="PK736_04590"
     CDS             complement(8052..8456)
                     /locus_tag="PK736_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRC32700.1"
                     /translation="MKLQRRNRIASEVYSHSLNDIMFFLLLFFLIAATMATPNVLKML
                     LPNSKTSSQQVKHPVTIAISEDLQYAVNSQIVPVEQLEATIANAIAGQVDPTAILKVD
                     KNIQVQSLVDILDIGNRLKIKMVLATSKPKQN"
     gene            complement(8463..9167)
                     /locus_tag="PK736_04595"
     CDS             complement(8463..9167)
                     /locus_tag="PK736_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRC32701.1"
                     /translation="MINTVLLQVMQNTVASDSLGTTPPPVTETSMPYLEVAMKGGPIM
                     IPIALLLLVAIYIFFERFYTIRKSSHIDMNFMNQIRDFVHNGNIESAKSLCKNTQSPV
                     ARMIEKGVMRLGRPIRDIEGAIENVGKLEIYKMEKNLSILGTIAGIAPMFGFLGTIFG
                     VIKIFYQISLQNSLEINTISGGLYIKMITSAAGLLVGICAYAAYHYLIILIDRVVNKM
                     EINAVEFIDLLEEPVK"
     gene            9377..>10006
                     /locus_tag="PK736_04600"
     CDS             9377..>10006
                     /locus_tag="PK736_04600"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HRC32702.1"
                     /translation="MLLLVCMCNVGMAQTRLQKFNNHAQTFFGKYVANGLVDYKTVKQ
                     NPDALNNLVTEIKFVSINNYSATRKKSFYINAYNVAVIWNVIQHYPIASPMDVKDFFD
                     EKIITIGGENLSLNDIENKKIRPVYHDARIHFALVCAAMGCPKITDNAIMYTTIDAQL
                     NMLTTSAINDATFIRVDNEKQTVLISEIFKWYEKDFTSNGNNILAYINQY"
BASE COUNT         3048 a         1837 c         1942 g         3160 t
ORIGIN      
        1 ttacgccaaa aagcaaaacg aaaaattgca actgcaagca tgtacccaaa tccagccgat
       61 gaaaaagtaa cattcaacta ttattcaaac gcaagccaaa aattaaaaat agaattgtat
      121 aattcttttg ggcaaaaagt ttacgataat aatttatcaa acacgagtac ggcttttgat
      181 ttgagtttaa agttgtatgc tcctggaata tattgttgta aaatcttaag tgatgggcaa
      241 agtattttat ctgaaaaatt agtaataata catgctcatg aataatataa tcaaaagctt
      301 gtttacatta ttaattgttt gttcactaaa ttgtttttcg caacagggta taaataattt
      361 atggatagaa ggatatggaa acgattctgt tgttttgcct tttggcaata cagatattga
      421 tttttttaac ggaaatccaa ccataacata caatatcaga ggacagcatc ttgctcatac
      481 tcattgcaat ataagtgatt cacttggcaa tttaaagttt tatacaaatg gctatgatat
      541 annnnnnnnn nnnnnnnnnn ttgatgcctc agatagtgtt atgctcaatg gtgattctat
      601 aagcccgtca tcaaagcaaa atgtatttcc ttatggtctg gatgtgccac aagccaatct
      661 tgtcatccct aaccccactg aaaataacaa atactattta tttcatagta caaatgatgc
      721 ttgggcttac aggtattgtt taaatttaat gtatagcgag atagatatga ctttgaataa
      781 tggattaggc gctgtgacag caaataaaaa tgttatactt ataaatgact cacttaacct
      841 tggcaagata gcagcttgca aacatggcaa tgggcgagat tggtgggtta tttgtatgcg
      901 ctcttacagc aatatttttt acaaactttt ggtaacacct tatggcataa gcgcacccga
      961 tacgcaagca attgggcaaa taagaaaaca aggttggggt gaattgaaat tttcaagcgg
     1021 tgggacaaag ttaggctatg ctcaagctga ttatgtaaca aatggccaca tagagctttt
     1081 tgattttgat agatgcagtg gcttactttc taatgctcaa catattatta ttcctcaagg
     1141 acttagcttt ggaggtggta tagaattttc acccaatgaa agtttggctt atgttactaa
     1201 tgcagatagt atttatcagt ttgatttata ttcaactgat atttcaaatt caaggattat
     1261 tgtttctgta agtgatggtt atattgatac cattatgaat actaattttt tggttggttt
     1321 catgaacaca aaactcgccc ccgatggtaa aatttacatt acaagcggca acagcaccca
     1381 ctatatgagt gtgatagacc agccagatag tcctggactt gcatgtaatg taatgcaaca
     1441 tgcagtgcag ttgccagctt tgtattttaa tactttaccc aatcacccta attatttttt
     1501 gggggcaaat ggaatgtgca ataatttggg cctccccatc ccctccaaag gaggggctaa
     1561 gaaaatacaa gtgtttggta accctacaca tgataaatta acattgtggt ttccggtaga
     1621 taaggatgtg ggttggttag agatttatga tgttaacggt gagtgtatac gtagggagcg
     1681 agtaagtcag tggagtcagt ataagactgt ggatataagt ttgtttgctg agggggttta
     1741 tttttgtagg atggtttggc ctgatggtga ggggagtgtt aaggtggtta ggttggagta
     1801 gccggtaagt cgcaaaaccc ctaaatcccc acttggggcc ttgcccattc gcatctttaa
     1861 agtttttaga taaataaatt gaacagcttt gtaaaaaaca aaaaccaaat gacaccaaaa
     1921 gcccagcgca gtgcaggtaa gtcttcgtag gcctgcaccg accgtagtgt cggtgggttt
     1981 ggtgtgcatt cttcgatgta aacggtgagt gtatacgtag cgagtaaata aaaagagcat
     2041 aaatgaaaca aggatattgg atacgcaatc aaaaattact ccccatattt caaatgcatc
     2101 acttttgttg gttgcctttt ttgcacaact acaacaaata aattatcgaa ctcatctaga
     2161 ggaatttcgt gataaggcat ggtgccacca aggttacgaa tcatggcagg aatgttatcg
     2221 ctgtggccta ctaccattac attttttgct tcgtaatctt cggccaatac ttttgccatg
     2281 tacaccgtat cgtggtaatt gtacacaaca ctgtcaatat tatttaaaag tcgcagaggc
     2341 tcagcagtgc ttgctgcacg aggtacattg gtggtataaa tctttgcaaa ttttacattt
     2401 tgtaatgtgt gtgccaattg ttgtgcacgt aatcgccctt gagcattaag gtcagcattt
     2461 ggcatggcag actccttctc gcaatggcga acaaaaaaaa tggtagtaga tggcgcacac
     2521 gactgaaaca ttgcagcaag tataagcagg aaaattaatt tacacatagc gtttgtcaaa
     2581 atattttttt ttgttttaga attattgttt ggcaatataa atattatccg tacttttcaa
     2641 ctcgaaaaac tattaaacaa aaatcatatg agaaaaaaac tactaattct gtctttattc
     2701 tgtttggctt tattcagttt aaaagcccaa actaatggtg gccccgatgc atacggctac
     2761 acctggcgcg atagtaacga cccaaatggt cagccatata attggattga tattacaaat
     2821 ttaccatttg taaattctgt aaaattgttg gcagatgata atgtggtggg tccttttccc
     2881 atgggcttca attttcatta ttactggtat gatgttgatg agtttcgtat tgggtccaat
     2941 ggctacattt ctttcaacaa tggaaatatg gctgttccat ttcctcctat tccacagccg
     3001 agtaagctaa ataattatat tgctgctttt ggcagcgatt taagttttgc cgatgcgggc
     3061 aatcccggtg aatgctattt ttggaaaagc ccgggcaacg atacattaat tgtatcatat
     3121 ataaatgttc cgttttggga tgccagtctt ttgtatgatg gctctaatac atttcagatt
     3181 attttgagtg cgttagatag ttctatcaca tttcaatatc aagaccaaca aggagtatac
     3241 aatggccttt ttgccgattt tttaacagtt ggtatcgaga acaattcagg caatataggg
     3301 cttatgcatt cgcatgatat ttatccggta gcaagttatt ctataaagtt ttaccctcca
     3361 tcaacagtaa cgtatacagc tatagatgct acttgcgatt gggttgataa tgtttcttcg
     3421 ggcggaagat ttttaacaaa gaatggaaca ccctatgaaa tgaaatcgaa catacgcaac
     3481 tatggcaata ccgtacttgc ccctttcagt gttttatcag aaataaaatc gggattctca
     3541 caagtggttg ccggtactgc cactaccgat actttaattc cgggtcaaag tcaaatatta
     3601 acttatccca ataatacttg gaatcacaat acaacaggta ctttcagtat gattaccaat
     3661 acacaactgg caaacgattt ggtaccatca aataataaag atacctgcga atttgtagtc
     3721 attgattcca ccactgcaag ccaatggtta tcgtttgacg atggctctgt aggtggcgta
     3781 gggttaagtt ggaatggcgg tggccccggt gaaggcgttg gtatgcaatt tcccttgcca
     3841 tattttccta tccagataga tgagatacgt gcattagtag ttgccaatcc aactcctgag
     3901 ggtatggcat taaaaataat tgacgatgat ggcgttggtg gaacaccact cactcaactc
     3961 gattcggtgt gggttgatcc ttttagtgtg caggttggca catatacaac agtacagtta
     4021 accaatccaa taataaaaac ttcgggttcg gtttatgtaa tgtgggaaat gggtggtaat
     4081 ggaatttcaa ttggacaaaa tgtaacacgc ccaatttcga atcgttcatt cgaagtactt
     4141 ggtggttcgt ggtctattta tcgtagtcgc gaagacgaag attttatcat caacataaag
     4201 gtttcggacc cgacagttgg cattgcagaa caaaatcctt tggccacagt ttttggaaat
     4261 atgtttccta atccggcaca aaattttgtg aatataaatt acaacacctc aacaatcatc
     4321 aacaatatta ctttggaaat atgcgatatg caaggccgca tagttcaaac aaaaaatatt
     4381 gccagtttgt atggcgaggg aagcatgaaa gtaagtattg ccgaattgtc tgccggtatg
     4441 tatatttgta aaatgagcgt taacgataaa tcgataaata agcgattcac aattgtgcga
     4501 taaagatttc tgccccttaa tagtagccct gcgcgatgaa taatctgcag ggcttttttt
     4561 atgttaataa aatgcaagaa tattaatatt cgttttaccg aaaactttaa atttgcgcaa
     4621 caatgaatta atgtatccat caatattttt tatatggaat taccaaaggg caaacttatt
     4681 tctattgggg gggcagaaga taaaggcacc gaagcagagc cgaattttac attaaaaaac
     4741 aacttgaatt ttttcgagtt gcagattcta cagcgtattg tacttgaaac aggcggtggc
     4801 gaaaaacaca ttgaagtgat aaccacagca tcgtctattc ccgaagaagt tggtgaaggc
     4861 tatttgaaag cgtttggcaa attgggatgc aaaaatgttc aaataatgca tatcaaaaaa
     4921 cgcgaggaca cccaaaatcc tgaatttata gaacgcataa agacatgtga tactgttatg
     4981 cttacaggtg gaaatcaatt gcgtttatca acaataattg gaggaaccga gattcataaa
     5041 atactgatgc atcgctacat ggaagaagaa aaaatgttga ttgccggtac aagtgccggt
     5101 gccatggcca tgagtaatac aatgatatat cagggcagca gcagcgaagc acttttgaaa
     5161 ggagaagtaa aacttacaac aggtttgggc ttaattaaag atgtaataat cgatacacat
     5221 tttgtaaagc gcggccggtt tggtcgattg gccgaagctg ttgccagcaa tccatcgtgc
     5281 atcggaattg gcttgggtga agatacaggc ctgcttatta cagaaggaac attgatggag
     5341 gccattggtt cggggttggt aataatcatt gatggtcaca atattaagta caccaatatt
     5401 gccgatgtac aatatggttc acctataagc atcgaaaatc ttgtggtgca tgtaatggcc
     5461 aaaggcaacc atttcgattt aaaacagcac cgttttttcg aagacgtgat taatgtatcg
     5521 aaataatttc gcgaccactt ttatatcact tctgccacaa caaaagtgct gccaccaata
     5581 aaaataaaat cttgttgcga tgcacttttt ctcgcagcat tgtaagctaa ttttactgtt
     5641 cgataagctt tacctttcaa attaaatgct tgcgctttta ttagcaattg ctctgcatca
     5701 aggccccggg gaatattggg tttgcaaaaa taataggttg catttggggg cattattttc
     5761 agaatagatg ctatatcttt atcgttgacc atgccaatca ctatatgcaa ttgtttgaat
     5821 ttgtttggtt caatttgttt caatatcatc acaatgccgg caacattatg agctgtatcg
     5881 caaacagtca ttggcttgcc ggcaatcact tgccatcggc cctgcaagcc tgttgaagca
     5941 gaaacacttt taagtcctgc ataaatatcg cgaggcttta ttttgaaaaa ctcttgcagc
     6001 acttcaacac tttgcaaaac agtgcataca tttttcaatt gataatcacc ggtgagatcg
     6061 agctgcaact tttcatacaa ttttttcttt cctttattca catcaagctt cagcgtagtt
     6121 ccgcttttta tttttgtaat ggatttacgg accgtaaaat tttgtgatgc aaaaacaatt
     6181 ggagccttat tttttctggc aaactgttcg aatattttcg ctgtagcttc gtcatgttca
     6241 cctattacaa caggaacacc gggtttaata attccggctt tttcgcgtgc gatttttgca
     6301 acggtattgc caagcaaatt tatatggtct aaagaaatat ttgtgatcac cgataaaacg
     6361 ggcgttatca cattagtaga atctaacctg ccaccaagcc cggtttcgat aatagcgata
     6421 tcgacatttt tatctttaaa atattggaat gccaaaccaa cagtccattc aaaaaatgat
     6481 ggctttatgg tttctaactc ttgtttattt ttttgcacga aatcggttac gtattttttg
     6541 tcaatcattt ttccatcgat acgaatacgc tcgcgaaaat cttttaagtg gggcgaagta
     6601 tacaatcctg ttttataacc cgcctgttgc aatatggaag caaggctatg cgacacgctg
     6661 cctttgccat ttgtaccggc aatgtgtaca gttaaaaaat tttgatgtgg gtgctgcaac
     6721 acttccatta tacgttgcgt gttatcgagg ttggctttgt atgcagcctc gcctattctg
     6781 tgatacatag gaagttgagc aaaaaggtat tctaaagtgc tattaaatga tgacataaaa
     6841 aagttttaag taaaggtgtt gcaaataaaa caaaaatgtt gattgataat atctttcaga
     6901 attataaata ttctaatagg tgacactaga agaagttgaa aaccacattc atggtattat
     6961 aaaattgcac gattggcatt cgaggtaaaa aaacaccatt tgaactttaa aaaactaaaa
     7021 aaaaacactt cagtaacaat gaccctatac gtttcaaaaa ggtaaaaatt tgaaacctaa
     7081 ttcattcaaa aaaaagcctg accctacttc ttatatttat aaacaaaagt aatagtgcct
     7141 ttctgtaatt cgctgccatc gggactggcg ttgaacttat attctttggc ttttaatttc
     7201 gacagctcgt ataaatgttt tgatgtagtg gtagaacctt gcccaccggg acttgcaatt
     7261 accacattgc catttttatc tacagtaatg tcaactacta cagtgcctgt ttcctgagaa
     7321 ttgtcggaca cctgcggacg cgaacgtatt tcacgatttg tgagatttac cttcattcca
     7381 ttacctccac caccggggcc tgtgccattt ccggtgccat cacctattcc atcgcctttg
     7441 ccgccaccat tgccaccgcc acttccatcg cctttgccat aaccaccaaa aggatttccg
     7501 ccaggagtac cttgatcgcc tttgcctgta ttggctgttc cctgtgaagt gctgttgttg
     7561 cttttacccg gatacaatgc attggggtcg ggcttgcgtt cgggctcttt ttttactact
     7621 actttttcgg gcacaactgt ttttaccggt tctattttct ttactaccgg tttctcggtt
     7681 tctttctttg gtttgggtat ctcttcaatt tttgccgact cctcattctc ttgtgtcata
     7741 atttcttcgg gagttgcggg agttggcttt gccgtttctt ccacagggtt gggcgttgtg
     7801 gtagtttctt cgctcatagg ttgtatgttg ccgcttgcaa gttccacgta gccaaggtta
     7861 cctccgcctc cacctccgcc accaaacggt ggtatctccg tatacagttt tagtataaat
     7921 agcaatagta taagtatacc ataaacaatg gctgtcgaaa tgtaagtgcg tgttcttatt
     7981 tgtgcttcca taactttttt gttttacgat gatatgaaaa catgaattcc ataaaatcaa
     8041 ttgtcagcat tctaattttg ttttggtttg ctggtagcca acaccatttt tattttcaac
     8101 ctgttgccaa tgtcaagaat atcgacaagg ctttgtacct gaatattttt atccactttt
     8161 aatatagcag taggatctac ctgacctgct atggcatttg caatagttgc ttcaagttgc
     8221 tcaaccggca ctatctggct atttacggca tattgcaaat cttcggatat agcaatagtt
     8281 acgggatgct tcacttgttg cgaacttgtt ttactgtttg gcaacaacat tttcaataca
     8341 ttgggtgtag ccatagttgc agcaataaga aaaaacaata acaggaaaaa catgatatcg
     8401 tttaacgagt gcgagtatac ttcggatgca atgcgattgc gcctttgtaa tttcatgatt
     8461 atttatttta caggttcttc aagcaaatcg ataaactcta cagcattaat ttccattttg
     8521 tttaccacac gatcaataag aatgataagg tagtgatatg cagcataagc acaaatacct
     8581 accagcaaac cggcagcact ggttatcatt ttaatgtaca gtccgcccga gatggtattt
     8641 atctccaaac tgttttgcaa tgatatttga taaaagattt ttattacccc gaatattgtt
     8701 cctagaaaac caaacatggg ggcaatacct gcaatggtac caagtatgct gagatttttt
     8761 tccattttat atatctcgag tttgcccaca ttctctatgg caccctctat atcgcgaata
     8821 ggccgaccta agcgcatcac ccctttttca atcatgcgtg caactggtga ttgggtattc
     8881 ttgcaaaggc tttttgccga ttctatattt ccgttatgca caaaatcgcg aatttgattc
     8941 ataaaattca tatcgatgtg tgacgatttg cggatagtat aaaaacgctc gaagaatata
     9001 tatatcgcca caagcagcaa tagtgcaata ggaatcatga tgggcccacc tttcattgct
     9061 acctctaagt aaggcatgga tgtttcggta acgggtggcg gagtggtgcc caacgaatcg
     9121 ctggccacgg tgttttgcat tacttgcaat agaactgtat tgatcattat taaagttatt
     9181 ttaattcaac aaaattagaa gtacgcgagg gtaactgtct ttactataac tttagttatt
     9241 aacatgatta tgttagccta aacgacacat tgttttgaat gttgtatgct acccgacaat
     9301 ggcacaattt aaaaggcatt acatttgctt tacaatttta aaatcaaagt agatgaacct
     9361 ttataaatca gcgataatgc tactcttagt atgtatgtgc aatgttggaa tggcacaaac
     9421 ccgtttacaa aagtttaaca atcatgcaca aacattcttt ggcaaatacg tagcaaatgg
     9481 attggtagac tataaaacag tgaaacaaaa tcccgatgca ttaaacaatc ttgtaacaga
     9541 aataaaattt gtgagcatca ataactacag cgccaccagg aaaaaatctt tctacattaa
     9601 tgcttataat gttgccgtga tttggaatgt aatacagcac taccctattg catcacctat
     9661 ggatgtgaaa gatttttttg atgaaaaaat aattacaatt ggtggcgaaa atttatctct
     9721 taacgatatt gaaaacaaaa aaatacggcc ggtgtatcat gatgcgcgca ttcactttgc
     9781 attggtatgt gccgcaatgg gttgccccaa aattactgat aatgccatta tgtataccac
     9841 aattgatgcg caattgaaca tgcttacaac atcagctatt aacgatgcta cttttattcg
     9901 cgttgataac gaaaaacaaa ctgtacttat atctgaaata tttaaatggt atgaaaaaga
     9961 ttttacttct aacggtaaca atatactcgc ttacataaac caatat
//
LOCUS       DAORQA010000050         9993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1792_length_9993_cov_9.218958, whole genome shotgun sequence.
ACCESSION   DAORQA010000050 DAORQA010000000
VERSION     DAORQA010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9993
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1792_length_9993_cov_9.218958"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="PK736_04605"
     CDS             <1..1092
                     /locus_tag="PK736_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32703.1"
                     /translation="QQQYQFGINNYLLAVEFSSPDTGYVGGGLGIFKTVDAGSNWSLI
                     LNSFSNYQIESIAFAPKNKNKITAVGGNGCIFTTIDAGQAWQKVPPILLFGDSLYDLK
                     KVVYLDDSTLVAVGGVDYLQLGIVIKSFDNGYTWNKIFESDNFPQLTDMFFLNTNEAW
                     ATGTYFNNNPFYKRGRLLKTIDGGVSWHILDSIAGGGWSNYPLVMRSVVFTSFLRGYA
                     VGYQNQTIFATVDGGLNWVNQNTSNMVFPFSIFFTDSLTGYIVGDSGKVLKTTNGGVG
                     FFEQNVNTNSLSIYPSPCNGKFTLSVAEKPNKSKSINVSTITGKLIYKKENVFDNVFQ
                     VDLTKEARGIYFVKVIQQNNVMVGKVILE"
     gene            1469..2386
                     /locus_tag="PK736_04610"
     CDS             1469..2386
                     /locus_tag="PK736_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32704.1"
                     /translation="MRKINTTILCIAIIVGIGCKNAMSQYAWPEKAKCETLKSRTLAV
                     RLFTDANGEYTAYNDAIKKAYNEEWKITAVEFFSPKDYADLVKEGNTKYAVVYAGDAL
                     STRTLERVKGSEIIHSKEFRFAHFDVTLANITGADALDPVTVVSFANDDLLPSEFFFA
                     AQQFVLLVNASLNDVSGLTFYDHEKNIEYIKSKKLAVPDELFNEKDLVKIADKYEFPN
                     KVVTNKELNDLVLAKNEEYVYPKIIWSNMHSAYGWVMISAKDGSLRSFMSFGGIKTNS
                     EQKANEVLKTGQLKNALSSFMQKVNNRYK"
     gene            2470..2919
                     /gene="lysM"
                     /locus_tag="PK736_04615"
     CDS             2470..2919
                     /gene="lysM"
                     /locus_tag="PK736_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HRC32705.1"
                     /translation="MGLLSFIKNAGSKLFGGKETEEEKSKIVTEHVNSFKLPNCDITV
                     SIQGDTATLSGTAETQDVKNRAMAAAGNVNGVSEVIDNVTVKEPPPAVELEKQFYTVE
                     KGDSLSKIAKAVYGDPMKYPIIFEANKPMLTNPDLIYPGQRLVIPVM"
     gene            complement(3050..4594)
                     /locus_tag="PK736_04620"
     CDS             complement(3050..4594)
                     /locus_tag="PK736_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32706.1"
                     /translation="MKAKLMALVMLLSAIFSKAQNTIDKTILYNNTVAKAIQNASQQN
                     ADVVNLNSNTKKGNTINSFLPDADDDGMPDTWETLYGLDPNDATDAWGDIDSDNVLNL
                     FEYQLGSVANDPASPVTIDVNAGGNIATAIDNANAGELIRVETGTYNLNYQGFTPKTV
                     MIQGGWNNTFTSHNPYTTPSILNGKSTAEIFYFECSGDTVNIVFDGLQLINGGGFFGA
                     VAFICDNGATANLCMKDVTIAQTKAKSAFTGCVTITHWKGSISDVRIANLLIANCRNS
                     GIYNQTTYGSNAKWNVVNSTITNIIDTASADCYGIKGLSLDSTINTNIQINVKNAIIR
                     DNSDAAIYFTNGGSSLGSSAAASYCDFDTVVFINGVVYTPGAGMIDANPQFVNPTADF
                     HLTALSPCIDAGINVGLPFVGAAPDMGIFEYGTVGIESVNHSSSLINIYPNPVANILF
                     IDGVFMTDKNAIHINDVLGNVVCEWNNINTSTPSLDVSKLSKGIYFITIENENGLSVM
                     RVMKTD"
     gene            5578..8661
                     /locus_tag="PK736_04625"
     CDS             5578..8661
                     /locus_tag="PK736_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC32707.1"
                     /translation="MSDKLLKALMQLFAIVANAGRLTEQGRAIVENFLQQQLNPSLVQ
                     LYLAEFDRDLQLLQGKAEEGKTKKRVSVNSVKVLRICTEINSELNQKQKQIVLIRLIE
                     FAYSSDESIAELELEFLSTVANVFNISNEEFEISQSYASHRKINEIVDVPEILLINKQ
                     TESPFVNARHVKHEYFDGDLLLLHIKSTDIIFARYFGKGKITINGSEVMNDAAHVLSQ
                     GSVIRSGKAQPIYYNDIIRSFLNDAQQSEIKFSVSNVEYVFHSGKVGLHNTNFEASSG
                     NLIGIMGGSGAGKSTLLNILNSNLSPARGEVLINGINLHTQKEKLDGIIGFVPQDDLL
                     MEDLTVFQNLFYNSKLCFGNLSNIEIVNKVYAMLQSLGLLEIKDLKVGDPLNKTISGG
                     QRKRLNIALELIRTPSILFVDEPTSGLSSLDSETVMDLLKQLAVSGKLVFVVIHQPSS
                     EIFKLFDKLLILDTGGYPIYYGNPVDSLIHFKQHAMYADSNVSMCESCGNINPEQVFS
                     IIETKVVDEFGKLTNSRKFLPTDWYNFHNSKNITNDTDIKPTNEIEKGDYHKPSRWKQ
                     IGVYIMRDINSKLNNTQYILINFLEAPVLAFILAYFLRYSKNNGAYIFSENVNLPAYI
                     FMCVIVALFMGLTSSAEEIIRDRKILKREKFLNLSRFSYLFSKTSIMFFISAIQTATF
                     VMVGNFMFGIHDMYLCYWLVLFSASCFANLMGLNISATFNSVVTIYILIPFLIIPQII
                     LSGVIVKFYNLNPVVTTQNKVPPIGEIMASRWAFEALAVHQFKENKYAKLFFEIDKAT
                     SNANFKKGPWMEKMNDKLDSLHGKLYGAQQQYQLQLFNNELQKELAALGNYGGAYSFN
                     YNRVIDDAAYNKLKEEFKNISKQYNVIFNTAAAKREAIVNRLGEKNITLLKQNYNNES
                     LNDMVTDKLSDVMITEEDGELIQKFRPVYLDGSSHSLVRAQFYVSRKNVFGHYISTFA
                     VNIIVLWLMTLFLCVALYFNWLKIVLHYGSALVDLVKAKLNNIRIFRKKSVPTVS"
     gene            complement(9052..9432)
                     /locus_tag="PK736_04630"
     CDS             complement(9052..9432)
                     /locus_tag="PK736_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HRC32708.1"
                     /translation="MENLIIEPSVKTPTINFYADNGILEIKGKSIPENSIEFYRPVYE
                     WLDKYAQSPAIATNIMVQLEYFNTSSSKCLLDVFRKLEILQGYGKSTVTVTWMYEEED
                     EDMMEAGEDYQTIVKLPFTISKMV"
     gene            complement(9445..>9993)
                     /locus_tag="PK736_04635"
     CDS             complement(9445..>9993)
                     /locus_tag="PK736_04635"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HRC32709.1"
                     /translation="DLYTLYQNMVRDDIILSFKGDITQDLLTSVYQIMESHLDTSLEE
                     PRRKKKLYHILVECLQNVYHHMEALKNDTKNTVDEETNSAIFMIARGDETGYRILTGN
                     HIRTANVDDLRNKIDKINAMTHDELRVFYRQQLSATELSDKGGAGLGMIDIARKSGHK
                     IDYSFQPVTDTFSFFSLIVNIN"
BASE COUNT         3247 a         1767 c         1851 g         3128 t
ORIGIN      
        1 caacaacaat atcaattcgg aataaataat taccttttag cagttgaatt tagttcacct
       61 gataccggat atgtaggtgg tggtttagga atattcaaga cggttgatgc aggttcaaat
      121 tggagcttaa tattaaattc gttttcaaat tatcaaatag agtcaattgc ttttgctcca
      181 aagaataaaa acaaaatcac tgctgtaggt ggcaatgggt gcatttttac aacaatcgat
      241 gctggacaag cttggcaaaa agtaccacca attctattgt ttggtgattc tttgtatgac
      301 ttaaagaaag tagtttattt ggatgacagc actttggtgg ctgttggtgg tgtggattac
      361 cttcaacttg gcattgttat aaaatcattt gataatggtt atacatggaa taaaatattt
      421 gaatccgaca attttcctca actaacagat atgttttttt taaataccaa tgaggcatgg
      481 gcaactggaa catattttaa taataatcca ttttacaaac gtggtagact tttgaaaaca
      541 attgacggtg gcgtgtcttg gcatatactc gattcaattg caggaggagg atggtcaaac
      601 tacccattgg taatgcgttc agttgttttt acatcatttt tacgtggata cgcagttggg
      661 tatcaaaatc aaactatttt tgcaacagtt gatgggggtc ttaattgggt aaatcaaaat
      721 acttcaaata tggttttccc attttcgatt ttcttcaccg attccctcac aggttacata
      781 gttggcgata gtggcaaagt tttaaaaact accaatggcg gtgtaggatt ttttgagcaa
      841 aacgtaaata caaattcgct gtcaatttac ccttcacctt gtaatggaaa atttacttta
      901 agtgttgcag agaaaccaaa taaatcaaaa tccattaacg tatccaccat aactggaaaa
      961 ttaatttaca aaaaagaaaa tgtttttgat aatgtatttc aagtagattt gacaaaagaa
     1021 gcaagaggaa tttattttgt aaaagttatt cagcaaaata atgtgatggt gggtaaggta
     1081 attttagaat gatgttttgt tagattatag aatctaaaat aaaaaatttc agtttaaaag
     1141 tgtattttca aatttataat ggctttggtg tttttttaat atcgggttag gccaatttaa
     1201 tttctctaaa aactaaacgc aataccacat tgtgtataaa aaccttcggt gcaacgctca
     1261 gtggaaatgt tgaaacacaa gatttaaaaa aactgagcaa ttgacatagc cagcaattta
     1321 actatgagag aatcatcaga tttaaagttg atgcaataat tttgtactaa cacttatctt
     1381 gtttttatat ttataatttg tattcatttt gaaaaaaatc cccttaatct ttatttgaat
     1441 aaacatgtat cttcgcaaaa ataacaatat gagaaaaata aacactacta tcttatgtat
     1501 tgcaataatt gttggtattg gttgtaagaa tgccatgtcg caatatgcat ggcccgagaa
     1561 agcaaaatgt gaaacattaa aatcgcgcac actcgcagtg cgattattta cagacgccaa
     1621 tggagaatac actgcctaca acgatgcaat aaaaaaagcc tacaacgaag aatggaaaat
     1681 aactgcggta gaattttttt cgccaaaaga ttatgctgat ctagtaaaag aaggaaacac
     1741 aaaatatgct gttgtgtatg ccggtgatgc gctcagtact cgtacactgg agcgtgtgaa
     1801 gggcagcgaa attattcatt ccaaagaatt tcgctttgcc cattttgatg taaccttagc
     1861 caacattacc ggtgccgatg cattagatcc tgtaactgtt gtttcgtttg ccaatgacga
     1921 tttattgcca tcggaatttt tctttgcagc tcaacaattt gtattgcttg taaatgcttc
     1981 gttgaatgac gtttcaggat taacttttta tgaccacgaa aaaaatattg aatatataaa
     2041 atcaaaaaaa ttggcagtgc ctgatgaact tttcaacgaa aaagatttag taaagattgc
     2101 cgataaatat gagtttccaa ataaagttgt caccaacaaa gaacttaacg atttggtatt
     2161 ggcaaaaaat gaagagtatg tatatccaaa aataatatgg tcgaatatgc acagtgctta
     2221 tggctgggtg atgataagtg ccaaggatgg ttcgttgcgt tcgtttatgt cgtttggtgg
     2281 cataaaaacc aacagcgaac aaaaagcaaa cgaagtatta aaaaccggac aattgaaaaa
     2341 tgccttgtcg agttttatgc aaaaagtaaa caatcgctat aaataacttt tcgaactttt
     2401 tttaataaca caataactat taaccaacaa cttttgattg ctttgcataa aatttaaaaa
     2461 taaataaaaa tgggattatt atcatttatc aaaaatgccg gcagtaaatt atttggcggc
     2521 aaagaaaccg aagaagaaaa atcgaaaatt gtaaccgaac atgtgaattc gtttaagctt
     2581 ccaaattgtg atatcacggt aagtatacaa ggagacacgg caacactcag tggcactgcc
     2641 gaaacacaag atgtaaagaa ccgggcaatg gccgcagcgg gaaatgtaaa cggagtgagt
     2701 gaagtgattg acaatgtaac cgtgaaagaa ccaccaccgg ctgtagagtt ggagaaacaa
     2761 ttttatactg ttgaaaaagg cgattcgctt tcgaaaattg caaaggccgt ttatggcgac
     2821 ccaatgaaat atcctatcat tttcgaagcc aataagccga tgctgaccaa tccggattta
     2881 atttatcccg gacaacggtt ggttattcct gtgatgtaaa atatttttat tgcttattaa
     2941 aatgcctatt tggattacca aataggcatt ttattttttt tgcttgtttg ccgttattac
     3001 atatcagcat tttttatttt ctcaaaaatg ttaaacctaa atattaatgc taatccgttt
     3061 tcataaccct cattacactt aatccatttt cgttttctat tgttataaaa tatattcctt
     3121 tggaaagttt gcttacatct aaagatggtg ttgatgtgtt tatattattc cattcacaaa
     3181 caacatttcc caatacatca tttatatgaa ttgcattttt atctgtcata aaaactccat
     3241 caataaataa aatatttgca accgggttgg ggtaaatatt tattaatgaa gaactgtgat
     3301 taacgctttc aattcccacg gtaccatatt caaaaattcc catatcgggt gctgcaccta
     3361 caaaaggcaa tcctacattt atgcctgcat caatacatgg actaagtgct gtgaggtgaa
     3421 aatcggccgt tgggtttaca aactgcggat ttgcatctat catacctgca ccgggtgtat
     3481 aaacaacacc atttataaaa accaccgtat cgaaatcgca ataacttgct gccgcactcg
     3541 aacccaaaga agagccgcca tttgtaaaat aaattgccgc atcactattg tcgcgaatga
     3601 tagcgttctt tacatttatt tgtatgttag tgttaatagt agagtcgagc gacaaccctt
     3661 ttataccata acagtcggca ctggcagtat ctataatatt tgtaatagtg ctgtttacca
     3721 cattccattt tgcattacta ccataagtag tttgattgta aatgcccgaa ttgcggcagt
     3781 tggcaataag caaatttgca attcgcacat cggatatact tcccttccaa tgagtaatgg
     3841 taacacaacc tgtaaatgca cttttggctt tcgtttgtgc aatagttaca tctttcatac
     3901 agaggttagc cgtggcacca ttgtcacaaa taaaagctac cgcaccaaaa aaaccaccac
     3961 catttataag ttgaaggcca tcaaacacaa tattaaccgt atcgccacta cactcaaaat
     4021 aaaaaatttc ggcagtgctt tttccattca atatacttgg cgtagtgtat gggttgtggc
     4081 ttgtaaatgt attgttccat ccaccttgta tcattacggt tttaggagta aagccctgat
     4141 agtttaaatt gtatgttccc gtttctacgc gaattaattc tcctgcattt gcattgtcga
     4201 tggcggtagc aatattgcca cctgcgttta catctatagt tacaggcgat gccgggtcgt
     4261 tggcaacact gcccaattga tattcgaaaa gattaagcac attgtcgctg tctatatcgc
     4321 cccatgcatc ggttgcatca tttgggtcga ggccataaag tgtttcccat gtatcgggca
     4381 taccatcatc atcggcatcg ggtaagaacg aattgatggt gttgcccttt tttgtattgc
     4441 tatttaggtt tacaacatcc gcattttgtt gcgatgcatt ttgtattgct ttagcaaccg
     4501 tgttattgta taaaatagtt ttatcaattg tgttttgtgc tttgctaaat attgctgaca
     4561 acaacataac caaagccatc agttttgctt tcatgaaata atcttagata aaatatttga
     4621 accaatttca gtttcgaaga gtgcgcaaaa ctaaatcatt ttccaaagcc ggtttttgat
     4681 ttacccattg tatgaagaaa gttgttgttt tgttcagaat gatttattgc tcaattgttt
     4741 cattgttgtt tttagaatga agttgaaagg ctattcagca ttgtctttaa ttttataccc
     4801 cctccaaaat ccaattgaaa ataatgcata tgaaataatg ccgagtaagg catgcatgaa
     4861 atatacctgg ctggattcag aactgaacag aattatttat tgtttgatgc taaaattcgt
     4921 tgaacccttt cgggttctgt ttgtgcgcat atccgcacca ccacaggtat ttacctgtgg
     4981 ttattcaagt tgaacccttt cagggttatt tgccattgca aggtgctaaa aaaaaggcgc
     5041 acagaaattt atcccaagca aaatataaat taatgcaagc gggaattgac aaaattattt
     5101 tgaattatac ttcacacggc tcaaattaat atatggtatg aacgaataac taatatacga
     5161 attcgtgtaa ttcgtatatt cgtaactatt tgtaattcgt tgatgccata accaaatgta
     5221 taattatatc ccattctaag tataaatatt cactcaactt tttcttctga tttatacacc
     5281 ctccaaaacc caattgaaaa cagtgcataa gaaataatgc cgagtatggc gtgcatgaaa
     5341 tataccttat gtggactccg ccactttttt tattttgaat tggaagcatg aatattaaac
     5401 atttaaaaat ttagcaataa cacttataat agcgggtgta actatttgaa catagatgag
     5461 aagaagtgtt tttaagcaat gcccaaaatg cttgaccacc aaaaataaat ttgcaatcaa
     5521 gcacgaaaaa atatcatctt tagcagccga ataaactaaa aggaaaaacc atcaccaatg
     5581 agcgataaat tactcaaggc cctaatgcag ctgtttgcca ttgttgccaa tgcaggcagg
     5641 cttaccgaac aggggcgcgc tatagtagaa aattttttgc aacaacaact caatccatct
     5701 ttagtacaat tatatcttgc agaattcgac cgcgatttgc aattgttgca gggtaaggcc
     5761 gaagagggca aaacaaaaaa acgcgtgtcg gtaaactccg taaaggtgtt gcgtatatgt
     5821 accgaaataa atagcgagct caatcaaaag caaaaacaaa ttgtacttat tcgcttaata
     5881 gaatttgctt attcgagcga tgaaagtatt gccgaactgg agcttgaatt tctttctacg
     5941 gttgccaatg tttttaatat cagtaacgaa gagttcgaaa tatcgcaatc atatgcatcg
     6001 caccgtaaga taaacgagat tgtggatgtt cccgaaatat tattaatcaa taaacaaacc
     6061 gaatcgccat ttgtaaatgc acggcatgta aagcacgaat atttcgatgg cgatttatta
     6121 ttgctgcata taaaatcgac cgacattatt tttgcacgct actttggcaa aggcaaaatt
     6181 acaatcaatg gcagcgaggt gatgaatgat gccgcacatg ttttgtcgca aggatcggtt
     6241 atccgttcgg gaaaggcaca acccatttat tacaacgata ttattcgcag ctttttgaac
     6301 gatgcccaac aaagcgaaat aaaattttcg gttagcaatg ttgaatatgt ttttcattcg
     6361 ggcaaagtag gattgcacaa tacaaatttc gaagcttcat cgggcaacct cattggtata
     6421 atgggtggca gcggtgccgg caaatcaaca ttgctcaaca tactcaacag taatcttagt
     6481 ccggcacgtg gcgaagtttt gataaatgga ataaacctgc atacacaaaa agaaaagctc
     6541 gatggtataa ttggttttgt gccacaagac gatttattga tggaggacct taccgttttt
     6601 caaaatttgt tttacaacag taaattatgt ttcggcaatt tatcgaatat cgaaattgta
     6661 aacaaagtgt atgcaatgtt gcagtcgctg ggcttactcg aaataaaaga tctaaaagtg
     6721 ggcgacccgc tcaataaaac aataagcgga ggtcagcgca agcgattaaa tattgcactc
     6781 gaattaattc gtactccttc tattttattt gttgacgaac ccacaagcgg actatcgtca
     6841 ctcgatagcg aaacggtaat ggacttgcta aagcaacttg ccgtaagtgg taaacttgtg
     6901 tttgtggtta tacaccaacc atcgtccgaa atttttaaac tcttcgataa gttattgata
     6961 ctcgatacag gaggctatcc aatttattat ggcaacccgg tcgattccct cattcatttt
     7021 aaacaacatg ccatgtatgc cgattccaat gtgagcatgt gcgaatcgtg cggcaatata
     7081 aatcccgagc aggttttttc aattatcgaa accaaggtgg tagatgagtt tggcaagttg
     7141 accaactcac gaaaattttt acctaccgac tggtataatt ttcataacag taaaaatatt
     7201 actaacgata ctgatataaa acctactaac gaaattgaaa aaggtgacta ccacaaacct
     7261 tcgcgctgga aacaaatagg tgtttacata atgcgcgata taaattcgaa actcaacaac
     7321 acgcaataca tacttatcaa ttttctggaa gcaccagtgc tcgcatttat acttgcttac
     7381 tttttgcgtt actctaaaaa taatggcgca tatattttta gcgaaaatgt aaacctgccg
     7441 gcatatattt ttatgtgtgt gatagttgct ttatttatgg gccttacatc gagtgccgaa
     7501 gaaattattc gcgatcgaaa aatattgaag cgcgaaaaat ttctcaacct cagccgcttt
     7561 agttatttgt tttcgaaaac gtccattatg tttttcattt cggcaataca aacggcaact
     7621 tttgtaatgg taggaaattt tatgtttggc attcatgata tgtacttgtg ttactggctt
     7681 gtattatttt cggcatcgtg ctttgcaaat ttgatggggc tcaatatttc ggcaacgttc
     7741 aattcggtag ttaccatcta tattttaata ccgtttttaa ttattccaca aataatattg
     7801 agcggggtga tagtaaaatt ttacaacctc aatccggtag ttaccacaca aaacaaagtg
     7861 ccgcccatag gagaaataat ggcttcgcgg tgggctttcg aggcgctggc cgtacatcag
     7921 tttaaagaaa ataaatatgc taaacttttt ttcgaaatag ataaagccac cagcaatgcc
     7981 aattttaaaa aaggcccatg gatggaaaaa atgaacgata aactcgattc gttgcatggc
     8041 aagttatatg gcgcacagca acaatatcaa ttacaattgt tcaacaacga attacaaaaa
     8101 gagcttgctg cattaggcaa ctatggcggt gcatattctt ttaattataa tagagttata
     8161 gatgatgcgg cttacaataa actgaaagaa gagtttaaaa acattagcaa gcagtacaat
     8221 gtaattttca atacagcagc agcaaaacgc gaagcaatag ttaacaggct gggcgaaaaa
     8281 aatattaccc tgctaaaaca aaactacaat aacgaaagcc tcaacgatat ggttaccgac
     8341 aaactgagtg atgtaatgat aacagaagaa gatggagagt tgatacaaaa atttcgcccg
     8401 gtgtatttag atgggtcgag tcattcgctt gtacgtgcac agttttatgt ttcgcgcaaa
     8461 aatgtttttg gccattacat aagtacgttt gcagtcaaca ttattgtact atggcttatg
     8521 actttgtttc tttgtgttgc cttatatttt aattggttga aaattgtatt acactacgga
     8581 agtgctttgg tcgatttagt aaaagccaaa ttaaacaata tccgaatttt tagaaaaaaa
     8641 tccgtgccta ctgtttcata aataaaatgt gccattgcaa tattttgaaa aggatggatt
     8701 gaaatccatc cctacaaaat tgagtcaagc ctaaggctct attgcttgcg cgaaaatgat
     8761 ctgcacagaa aaaattgcac aacgtaagtg ccgtaggcac gaagtatatt gtagccatga
     8821 gttttaaccc atggtatttc gtattgtaaa tgatattttt gtacagaagg atatcaatgt
     8881 ttgtggggac acaaacattg gcggagtggg gacacaaaca ttggcggagt ggggacacaa
     8941 atattggcta acgcgaactt tggtcaacac catcattggc gacacaaaca ttggctggcg
     9001 caaaaaaaaa tcctattaat aattgccttt cgtagaaaac aagatttgga actaaaccat
     9061 tttcgaaatc gtaaatggta gctttactat ggtttgataa tcttcaccgg cctccatcat
     9121 atcttcgtct tcttcttcgt acatccaggt tacggtaacg gtgcttttgc catagccttg
     9181 cagtatttca agtttacgaa acacatcgag caaacatttc gaagaacttg tattaaagta
     9241 ttcgagttgc accattatgt ttgtagctat tgcaggcgat tgggcatact tatctaacca
     9301 ttcataaaca gggcggtaaa actcaatact gttttcaggt atcgatttac cttttatttc
     9361 gagtatgcca ttatcggcat aaaaatttat tgttggtgtt tttacacttg gctctatgat
     9421 gagattttcc atttcgagta tcggctaatt aatatttact ataagactaa aaaaagagaa
     9481 tgtatctgta acgggttgaa agctataatc aatcttgtgg cccgatttac gtgctatatc
     9541 aatcatgccc aatcctgcac cgcctttgtc cgacagttcg gttgcgctta attgctggcg
     9601 atagaatacc cgcagttcat cgtgggtcat ggcatttatt ttatctattt tgttgcgcag
     9661 gtcatccaca tttgcagtac gtatatggtt accggtaagt atgcgataac ccgtttcatc
     9721 gccacgtgct atcataaaaa tggcactgtt ggtttcttcg tcaacggtat tttttgtatc
     9781 attctttaat gcttccatat ggtgatatac gttttgtaaa cattctacca gtatatgata
     9841 aagtttcttt tttctgcggg gttcttcgag cgatgtatcg aggtgcgact ccattatttg
     9901 atacaccgaa gttaacaggt cttgcgtaat atcacctttg aacgaaagga taatatcatc
     9961 gcgcaccata ttttgatata gggtatataa atc
//
LOCUS       DAORQA010000051         9952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1801_length_9952_cov_19.222088, whole genome shotgun sequence.
ACCESSION   DAORQA010000051 DAORQA010000000
VERSION     DAORQA010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9952
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1801_length_9952_cov_19.222088"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..3098
                     /gene="polA"
                     /locus_tag="PK736_04640"
     CDS             306..3098
                     /gene="polA"
                     /locus_tag="PK736_04640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRC32710.1"
                     /translation="MKKLFLLDALALIYRSYFAFSKNPRITSKGLNTSAMFGFTNTLL
                     EILNNEKPTHIGVAFDTSAPTQRHIDFVDYKAHREEMPEDLRISIPIIKDIIRAFNIP
                     VLEYDGFEADDIIGTLAKAAEREGFKVYMMTPDKDMCQLVSDNIFMYKPAKFGSPAEV
                     MGVAEVLQKYEIKRVDQVIDMLGLMGDSADNIPGIPGVGDKTARELLKQYDNVENVIA
                     NADNLKGALQRKVKEGAESAIISKKLATIWLDAPVPFVVSDLVVEPVNKEAIKTIFNE
                     LEFRQLTKRVLGEELPPATASSQGNLFDAPIEVSANKANDESEEVPELINYKTIADVA
                     HTYHLIDTKEKREALITQLLQQKEICFDTETTGIDANVAELVGMSFCYKPFEAYYIPF
                     PENYDEATKILHEFKPVFENDAIEKIGQNLKYDLLMLRWYDVEVKGKFFDTMLAHYLL
                     EPDMRHNMNVLSETYLRYTPVPIEKLIGEKKGGQLSMRTVAIEQISEYAAEDADVTFQ
                     LKNILEPELRKNNLYKLFEEVEIPLMSVLVAMEMEGVAIDPQFLNEYSVLLQNDIAVI
                     EKEIYEVCGLRFNIASPKQLGEVLFDHLKIDPKAKKTKTGQYATGEDVLSKLADKHAA
                     VAKVLDFRELVKLKNTYVDTLPEMINPRTHRVHTSFAQAVAATGRLSSNNPNLQNIPI
                     RTDRGKEIRKAFIARNEKFTLMSADYSQIELRIIAEFSKDESMIDAFKNGIDIHASTA
                     SKVYNVALADVTGEMRRNAKMVNFGIIYGISAFGLSQRLNIPRGEAKEIIDNYFAQYP
                     TIKAYMDGSIQQAREKGYVETILGRKRYLRDINSANAVVRGFAERNAINAPIQGSAAD
                     LIKVAMINIYNEFRTAKFNTKMTLQVHDELVFDVPLDEVEIVKPVIRKCMREAIKMTV
                     PLEVEIGVGHNWLEAH"
     gene            complement(3644..3940)
                     /locus_tag="PK736_04645"
     CDS             complement(3644..3940)
                     /locus_tag="PK736_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32711.1"
                     /translation="MGYKKIIVQKQVANSIAGIAWYIESKGMLKTADIFCDAVYDYIF
                     ELGNENRSYPFCRESKRKMAGLKCAQFKKKYTIAFKEYETKIIIVEFIPSKLIY"
     gene            complement(3922..4092)
                     /locus_tag="PK736_04650"
     CDS             complement(3922..4092)
                     /locus_tag="PK736_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32712.1"
                     /translation="MTQITIDKKKYVIIPEKEYQQLLKNAALKSKSDVGFTIEEARAY
                     SKKVISKWATKK"
     gene            complement(4451..7138)
                     /locus_tag="PK736_04655"
     CDS             complement(4451..7138)
                     /locus_tag="PK736_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32713.1"
                     /translation="MKKKLCTTLFFLLATFFCKGQYDIKWQNTIGGSDDEWLTCIIAC
                     SDGGHLLGGGSKSNISGDKTDSCYGEWDYWIIKIDSLGIIQWQNTIGGSGYEIISSIT
                     QASDGGYVLGGGSNSDISGNKTENSNGGQDYWVVKIDSLGTIEWQNTIGGSSNEWLSS
                     VVCSDDGGYVLGGSSQSNISGDKTENCIGPDDYWVIKIDSLGSIQWQNTIGGNGTDYL
                     NFITPVPDRGYLLGGTSSSNISGDKTENCIGFWGYWVVKIDSLGVIQWQNTIGGNATD
                     ELKSIIAAPDGGYVLGGYSNSNISGDKTENSKGGWDYWVVKIDSAGAMQWQNTIGGND
                     YDMLLSMSPAINGGYALAGISESNISGDKTENSNGLNDYWLVHIDSLGDIEAQSSIGG
                     GSDDRLHSISSATFGSYVFGGRSWSGVSGDKTEDSEGLYDYWVVKLSPSSRITGKIFI
                     DLNQNNIQDNGEANISGVKVTESSTGRYSYSQSDGSYSIGVFDSASYTVSPANTINYY
                     ASTPPTHTATFTATNQIDSLNDFAFQPTMIVNDLYISMHPLSPFRSGFNATYNIHYKN
                     IGTTTLSPIIYLYNDNKIDFVSASLLPNNVYADSVIWNLPALFPYSEGDITITVYVHQ
                     GLQIGTAIYSTAKIFPYTNDYNIANNAASWEVQTIGSYDPNEIIVNRDSLGVLELNPV
                     PPYLDYVIYFQNTGNDTAFNVKVENIIPYQLDLGSFEYLSSSTPVNISLNNQSRLAAF
                     EFPNIQLPDSNIDEPGSHGYIRYRIQPLKTLQVNDSILSLASIYFDFNSPVITNTAQT
                     LIGLNVFSSQNEIQIESNFSVMPNPAKNILSVTFKNQTQVNEHLEIISSTGKLLKKIN
                     VPTHTTKMEIDISKLSNGFYLLKYNQLENVGVLKFVVER"
     gene            complement(7290..8360)
                     /locus_tag="PK736_04660"
     CDS             complement(7290..8360)
                     /locus_tag="PK736_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HRC32714.1"
                     /translation="MQKHNIYDKNLLKQKLAQETFHRITLSFYRYVNIKEVEMFRDEL
                     YDAFDALNVFGRIYLAHEGINAQISVPQHNWEAFEQVVHNNALLQDVPFKIAVEDDPE
                     KIGASGKSFYKLIVRIKNKIVADGLPNDEIDFSKVGKHIDAIELNEVIQDPDTIVIDM
                     RNHYESEVGRFENALCPDADTFRDALPEAVKMVEDKKDHKIVLYCTGGIRCEKASAYF
                     KHHGFEDVNQLYGGIIEYARQIKREGLPSKFIGKNFVFDERMGERITDDVISVCHQCG
                     NPSDNHVNCANDDCHLLFIQCDECAQKMKGCCTPACMEIISMPIEEQRKQRKGKIKTD
                     AHSVYKSRLRPDLKKLIAEGKV"
     gene            complement(8409..9278)
                     /locus_tag="PK736_04665"
     CDS             complement(8409..9278)
                     /locus_tag="PK736_04665"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HRC32715.1"
                     /translation="MRTIIKMLNNYNHIRLTFVAVCMALLFFGCERDANVDVPLSKAK
                     LVMRSFISPTDSVIAVDIFASQPLFNSSQQNNKFDTSAAQVFISNGSVEFPLIYNSTK
                     RQFQINVLQAFPVTAGQTYFLRATHPSYDAVDASCTVPVSTIDNVKATVDITDPYEVF
                     ADVSFFDIAGQKNYYRVHAQNVWIPDFSYFGVDTVVFADFLFDVNNFYSDEGNDGKEI
                     KSKLLNYYSYHPGDSTDIYVLNVDENYYRFHRSVFTTGGGDPFSEATPAYSNINNGVG
                     IFAAYTQFRKRVF"
     gene            complement(9318..>9952)
                     /locus_tag="PK736_04670"
     CDS             complement(9318..>9952)
                     /locus_tag="PK736_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC32716.1"
                     /translation="QVDNDYNWEDKVTSGKGWSYGGELLLQRKEGKLTGWIAYTLSWT
                     QLQFDELNFGKKFYARFDRRHDISVVAIYHLREVKPEKNGITLSATWVYGTGNAITLP
                     LGYYDSPIHSPGNATSPDFNFYYPGNYLTEYTGRNEFRMKAYHRLDFSIQMIKKMKHH
                     TRTWELGLYNAYNRYNPFYYYVSEDLQGNGVLKQITLFPLIPSFSYNFKF"
BASE COUNT         3166 a         1895 c         1767 g         3124 t
ORIGIN      
        1 ccgagtacaa agaaactcat tttaactgta ttttagttat tggttattat gttattaggt
       61 tatttggtta ttgtgaaatt cagctattag ttgcaggcct ttagtgtttt gcttttaaac
      121 tcaggcaatt atttttagat gtaatccatc aatgtcaaaa tcaatttata gaatttctta
      181 aagttgttcg ttttaaattt taattcttaa ttttcaatat ttaattctcc atttttaaat
      241 tttaattctc catttttaat ttgttttaca atatctttga aaacctcaga ctataaaatt
      301 attttatgaa aaaacttttt ctcctcgatg cactggcatt aatttatcga tcatattttg
      361 catttagtaa aaatcctcgc attaccagca aaggtctcaa tacatcggcc atgtttggtt
      421 ttacaaatac gttgctcgaa atattgaaca acgaaaaacc aacacatatt ggagtagctt
      481 tcgatacttc ggcacctact cagcgccata tcgattttgt ggattacaaa gcgcaccgcg
      541 aagagatgcc cgaagacctg cgtatatcca tccctattat taaagatatt attcgcgcgt
      601 tcaatattcc tgtgttggaa tatgatggtt tcgaagccga tgatataatt ggcacattgg
      661 ccaaagccgc tgagcgcgag ggtttcaagg tgtatatgat gacacccgac aaagatatgt
      721 gtcaacttgt gagcgataat atttttatgt acaagccggc aaaatttggc agccctgccg
      781 aggtaatggg tgtggccgag gtattgcaga agtatgaaat aaagcgtgta gatcaggtaa
      841 tagatatgct tggcctgatg ggtgatagtg ccgataatat tcccggcata cccggtgtgg
      901 gcgacaaaac cgcccgcgaa cttttgaaac aatatgacaa tgttgaaaat gtaattgcca
      961 atgccgataa cctcaaaggc gcattgcaac gcaaagtaaa agaaggtgcc gagagtgcta
     1021 tcatttcgaa aaaacttgcc actatttggc tcgatgctcc tgtgcctttt gtagtaagcg
     1081 accttgtggt agaacctgta aataaagaag ccataaaaac catttttaat gaactcgaat
     1141 ttcgtcaact tacaaagcgt gtactgggcg aagaattgcc tcctgcaact gcatcctcgc
     1201 aaggaaattt atttgatgca ccaattgagg tttctgcaaa taaagcaaac gatgaatccg
     1261 aagaggtgcc cgaattaatt aattacaaaa ccattgctga tgttgcacat acttatcatt
     1321 taattgatac aaaagaaaaa cgcgaggcat taattacaca actattgcaa caaaaagaaa
     1381 tatgtttcga tacggaaaca acaggtattg atgccaatgt tgctgaactt gtaggaatgt
     1441 cgttttgcta caaacccttc gaagcttatt acattccatt tcctgaaaat tatgatgagg
     1501 ccacaaaaat tttacatgag tttaaacccg ttttcgaaaa cgatgcaata gaaaaaatag
     1561 gacagaattt gaaatacgat ttgcttatgt tgcgttggta tgatgttgaa gtgaaaggaa
     1621 aatttttcga taccatgctt gcacattatt tactggagcc cgatatgcgc cacaatatga
     1681 atgtgcttag cgaaacatat ttgcgttata ctcctgtgcc aatcgaaaaa cttattggcg
     1741 aaaaaaaagg cgggcagctg agtatgcgca ctgttgctat tgaacaaata tcggagtatg
     1801 ctgccgaaga tgctgatgtt acttttcaat tgaaaaatat tcttgagccc gagttgagaa
     1861 aaaataattt gtacaaactt ttcgaagaag tagaaattcc actgatgagc gtattggttg
     1921 caatggaaat ggaaggcgtt gccatcgatc cacaattttt aaacgagtac tcggtgctgt
     1981 tgcaaaatga tattgcagtt atagaaaaag aaatttacga agtgtgcggc ctgcgattta
     2041 atattgcttc gccaaagcaa ttgggcgaag tacttttcga tcatttgaag atagatccca
     2101 aagcaaaaaa aacaaaaaca ggacagtatg caacaggcga agatgtgctt agcaaacttg
     2161 ccgataaaca tgccgctgtt gcaaaggtgc tcgactttcg cgaattagta aagttgaaaa
     2221 atacttatgt ggatacacta cccgaaatga ttaaccctcg cacacaccgc gtacatacat
     2281 cgtttgcaca agctgtggcc gctacaggaa gattgagcag caacaatcca aatttgcaga
     2341 acattccaat acgcaccgac agaggaaaag aaattcgtaa agcctttata gcacgcaacg
     2401 aaaaatttac gctcatgagt gccgattact cgcaaattga gttgcgaata attgccgagt
     2461 ttagcaaaga tgaaagtatg attgatgcat ttaaaaatgg aatcgatata catgcaagca
     2521 cggcaagtaa agtttacaat gttgcccttg ccgatgttac cggagaaatg agacgcaatg
     2581 ctaaaatggt aaacttcgga atcatatatg gtataagcgc tttcggttta tcgcaacgat
     2641 tgaatatacc gcgaggcgaa gccaaagaaa ttatcgataa ttattttgca cagtacccca
     2701 ccataaaagc atacatggat ggcagcatac aacaagcccg cgaaaaaggc tatgtagaaa
     2761 ctatattagg acgcaagcga tatttacgcg atataaattc ggccaatgct gtggtgcgtg
     2821 gttttgccga acgtaacgct attaacgcac ccatacaagg cagcgctgcc gatttaatta
     2881 aagtggcaat gattaatatt tacaacgaat ttcgcacggc taaattcaac acaaaaatga
     2941 ctttacaagt acacgatgaa ctagtgtttg atgtaccact cgatgaagtt gaaattgtaa
     3001 aacctgtaat cagaaaatgt atgcgcgaag caattaaaat gaccgtaccc ctcgaagtag
     3061 aaattggtgt aggacacaat tggttggaag cgcattgatg ggggagttga aatactgtgc
     3121 gttaaaccca ctgttacaat atgcagcatc ccagcgggtt ttttgtgtgg caaaatcttt
     3181 cagtcaacat ttgctgcaca ttcaataatt ctaaagactc ggctcaactt tacaattcga
     3241 aacccacaag gtttttaaaa ccttgtgggt tttgtcgcga gttataaaat ttaagtttaa
     3301 gccatagatc cttaggctcg actctatttt gtaaggatgg aatttgatcc atcccaaaac
     3361 gaaattgcaa aaatttaaaa acatttaagt ttcaaaaacc caacaggcat ttttaaacta
     3421 ttaaaaaaca cttaacaact caaaagccta ttaactcaat aaccactctg cggccttgag
     3481 ttcagcctaa tatgcagaat agaaagcgga atagtaaaca caagcatttc gaacctaaat
     3541 cgcattgcag atgtattaga aattgcgcca ataaaattat ttagtaaaaa ttcaagtggt
     3601 atcaatgaga agtataataa aaatcttaca acaacaatta aaatcaataa attaattttg
     3661 atggaataaa ttcgactatg attatttttg tttcatattc cttaaaggcg atcgtatatt
     3721 tttttttgaa ttgcgcacac ttcaatcctg ccatttttct tttgctttct ctacaaaaag
     3781 gataagaacg attctcatta cccagttcga aaatataatc ataaacagca tcgcaaaata
     3841 tatctgccgt cttcaacatg ccttttgatt caatatacca tgcaattcca gcaatactat
     3901 tagcaacttg tttctgtaca attatttttt tgtagcccat ttgctaatca ctttcttgct
     3961 ataagcacgg gcttcttcga tagtaaaacc aacatcactt ttagatttta atgctgcatt
     4021 tttcaataat tgttgatatt ctttttctgg aataatcaca tacttctttt tatcaatcgt
     4081 tatttgagtc attgaacttt ttatttttac aaatgtaagt gtgaaatgta ttggttgcaa
     4141 ataaaaacaa gtcatttttt tagcactttc tactattcaa atataacact ttaataaatc
     4201 gggcaagttt tttcgcgcaa gcaatagaac tgtaaggtga ctcaacttta tcattcgaaa
     4261 cccacaaggt ttttaaaacc ttgcgggttt tattgcgcgt tataaaattt cagtttaagc
     4321 aatggaactt taggcgtggc tcaattatgc atggctggat tttaatccat ccgaaaacga
     4381 aaatgcaaaa aaaatcctga cagtttttag aagaccggta ggatttaaaa caagaaatgc
     4441 ggtgaatcaa ttaccgctcc accacaaatt tcaacacccc aacattttcc aattgattat
     4501 acttcaataa ataaaaaccg ttcgacaatt tactaatatc tatttccatt tttgttgtat
     4561 gtgttggcac atttattttt ttcaataatt ttcctgtaga tgaaataatt tctaaatgct
     4621 cattcacttg tgtttggttt ttgaatgtaa ctgataaaat attttttgcc ggattgggca
     4681 ttacactaaa attgctttca atttgaattt cgttttgaga actaaataca tttaaaccaa
     4741 ttaaagtttg ggctgtattt gtaattactg gcgaattgaa atcaaaataa atagatgcta
     4801 aacttaaaat agaatcgttc acttgtaaag tttttaatgg ttgtatgcga tatcgaatat
     4861 agccatggct gccgggttca tcaatattgc tgtcaggtaa ttgtatattt ggaaactcaa
     4921 atgcagcaag gcggctttgg ttattgaggc ttatgtttac aggtgttgac gatgataaat
     4981 attcaaacga accaaggtct aattgatatg gaataatatt ttctactttc acattgaatg
     5041 cagtatcatt gcctgtattt tgaaaataaa ttacataatc taaatatggt ggaacagggt
     5101 ttaattccaa aaccccaagc gaatctctat tcactattat ttcgtttggg tcatacgagc
     5161 ctattgtttg aacttcccaa cttgcagcat tgtttgcaat attgtaatcg ttggtgtatg
     5221 gaaatatttt tgcagttgaa taaattgctg tgccaatttg taaaccttga tgaacgtata
     5281 ctgttatagt tatatcaccc tcgctgtatg gaaaaagtgc cggcaaattc caaattacag
     5341 aatcggcata tacattattt ggaagtaaac ttgccgatac aaaatcaatt ttattatcgt
     5401 tgtacaaata aatgattggt gataaagtgg tagtgccaat atttttataa tgaatattgt
     5461 aagttgcatt aaacccgcta cggaaaggag aaagcggatg catgctgatg tataaatcat
     5521 tgacaatcat tgtaggttga aaggcaaaat cgtttaacga atctatttga tttgttgctg
     5581 taaatgttgc agtgtgtgtt ggtggagtgc ttgcataata atttattgta tttgcaggac
     5641 ttaccgtata gcttgcacta tcgaacacac caattgaata acttccgtcc gattgagaat
     5701 aagaatatcg ccctgtgctt gactctgtaa cttttacgcc agaaatatta gcttcgccat
     5761 tatcttgaat attattttga tttaagtcta tgaatatttt ccctgttatg cggcttgatg
     5821 gggaaagttt tactacccag tagtcgtaaa gcccttcgct gtcttctgtt ttatcgccag
     5881 aaacaccgga ccaagatctt cctccaaaaa cataacttcc aaaagttgca gagcttatgg
     5941 aatgcaaacg gtcatcactt cctccaccaa tagaactttg cgcttcaatg tctccaagac
     6001 tatcaatatg tacaagccaa taatcattaa gtccattact attctctgtt ttatctccag
     6061 aaatatttga ttcagatatt ccagccaacg cataaccacc attgattgct ggtgacatgg
     6121 aaagtaacat atcatagtca ttgcccccta ttgtattttg ccattgcata gcaccagcgc
     6181 tgtcaatttt cactacccaa taatcccatc cacctttact gttttctgtt ttgtcaccag
     6241 aaatatttga gtttgaatat cctcccaaaa cataacctcc atccggtgca gcaataattg
     6301 attttaactc gtctgtggca tttcctccaa tagtattttg ccattggata acaccaaggc
     6361 tgtcaatttt tactacccaa taaccccaaa agccaataca gttttccgtt ttgtcaccgg
     6421 aaatatttga agatgaagtt cctcccaaaa gatagcccct atcaggcact ggagtaatga
     6481 aatttaaata gtccgtgcca tttcctccaa ttgtattttg ccattgaata ctgccaagac
     6541 tatcaatttt tattacccaa taatcatcag ggccaataca gttttctgtt ttatcgccag
     6601 aaatatttga ctgtgaactt ccacctaaaa cataacctcc atcatcagaa caaacaacgg
     6661 aagataacca ttcattgctg gatccaccaa tggtattttg ccattcaatt gttcctaagc
     6721 tgtcaatctt aactacccaa taatcttgac caccattgct gttttctgtt ttgtttccag
     6781 aaatatctga atttgatcct cctcccaaaa cataaccacc atctgatgcc tgcgtaatag
     6841 aagaaataat ttcatagcca ctaccaccaa tcgtattttg ccattgaatg ataccaaggc
     6901 tgtcaatttt tattatccaa taatcccatt ctccataaca ggagtctgtt ttatctcctg
     6961 aaatatttga ttttgaacct cctcccaaaa gatggccccc atcggagcaa gcgataatgc
     7021 aagttagcca ttcatcatca cttcccccaa tagtattttg ccattttata tcatactgcc
     7081 ccttgcaaaa aaaagtagcg agtaaaaaaa ataatgtggt gcatagtttt tttttcattg
     7141 tcgtgagact ttttagcttt tatacaaggg taaatgtagg gaaataatta gaaaggtaaa
     7201 tcattttgca aaataaatcc taacaggttt ctaaaacccg tcaggattta aaacgtaata
     7261 acaaaatagc gtaataacaa ataaccaaat caaactttcc cttcagcaat caactttttc
     7321 aaatcggggc ggaggcggct tttgtacact gaatgtgcat cggttttaat tttgcctttg
     7381 cgttgtttgc gttgttcttc aattggcatc gaaataattt ccatacatgc aggtgtgcaa
     7441 catcctttca ttttttgcgc acattcatca cactgtataa aaagtaaatg gcaatcatcg
     7501 ttagcacaat ttacatgatt gtcgctggga ttgccgcatt gatgacaaac agaaattaca
     7561 tcatcggtta tgcgctcgcc catacgctca tcaaacacaa aatttttacc tataaatttc
     7621 gatggcaatc cttcgcgttt tatttgtcgt gcgtattcaa taatgccgcc ataaagttga
     7681 ttcacatctt cgaaaccgtg atgcttaaaa taggcactgg ctttttcgca acgaatacca
     7741 cctgtgcagt acagcactat tttgtggtcc tttttatctt caaccatttt tactgcttcg
     7801 ggtaaagcat cgcgaaaagt gtcggcatcg gggcacaggg cattttcaaa tcgacccact
     7861 tcactttcgt aatgattgcg catgtcaatt acaatggtgt cggggtcttg tattacttca
     7921 ttcaattcta tggcatcaat atgttttccc actttcgaaa aatcaatttc atcgttgggc
     7981 aatccatcgg ccacaatttt attttttatg cgcactatca gtttgtaaaa agatttacca
     8041 cttgccccga ttttttcggg gtcatcctca acagcaattt taaatggaac atcttgcagt
     8101 agtgcattgt tgtgtactac ttgttcaaat gcttcccaat tatgttgtgg cacacttatt
     8161 tgcgcattga taccttcatg agctaagtat attcgtccaa aaacatttaa ggcatcaaag
     8221 gcatcgtaca attcatcgcg aaacatttct acctctttaa tatttacata acggtaaaat
     8281 gaaagcgtaa tgcggtgaaa agtttcctgt gctaattttt gtttcagtaa gtttttatcg
     8341 taaatattat gcttttgcat gagagccaat ttttttgcaa atataaattt tataatgcga
     8401 aaataatatc aaaaaaccct cttcctaaac tgcgtataag ctgcaaaaat tcctacaccg
     8461 ttatttatat tggaataagc tggtgtggct tcggaaaaag gatcaccacc accggtagta
     8521 aaaaccgagc gatgaaaacg gtagtagttt tcgtcaacat tgagcacata aatatcggtg
     8581 ctatcaccgg ggtggtatga gtaataattt agcaacttcg attttatttc tttgccatcg
     8641 ttgccttcat cggagtaaaa attatttaca tcgaacaaaa aatctgcgaa caccactgta
     8701 tctacaccaa aataactaaa gtcgggaatc catacatttt gcgcatgcac ccggtaataa
     8761 tttttttgac ccgcaatatc aaagaatgat acatcagcaa acacttcata tgggtctgta
     8821 atgtcgactg ttgcttttac attgtcgata gttgaaacgg gcacagtaca acttgcatcc
     8881 acagcatcgt aacttgggtg agtggcgcgc aaaaaataag tttggccggc agtaaccgga
     8941 aacgcctgca atacattaat ttgaaattga cgttttgtag aattgtaaat gagaggaaac
     9001 tccacactgc cattgcttat aaaaacctgt gccgcggaag tatcgaactt gttattttgt
     9061 tgcgatgaat tgaacaatgg ttgtgatgcg aaaatatcca cggcaatcac actatcggtt
     9121 ggcgaaataa aactacgcat taccagtttg gctttcgata agggaacatc aacattggca
     9181 tcgcgctcgc atccaaaaaa taataaagcc atgcaaactg caacaaatgt taaacgtata
     9241 tggttatagt tgtttagcat tttaattatt gttctcaaat ttttcagtct atatacaatc
     9301 aacattttca tcattaatta aaatttaaag ttgtaagaga aagacgggat cagcggaaaa
     9361 agtgtaattt gttttaacac accattgcct tgcaaatcct cgcttacata ataataaaaa
     9421 ggattatagc ggttataggc attgtacaaa ccaagttccc atgtgcgggt gtggtgtttc
     9481 attttcttta tcatctgtat gctgaagtcg agtcggtgat aggctttcat cctgaactcg
     9541 ttgcggcctg tatactctgt aaggtagttg cccggataat aaaaattaaa atcaggcgat
     9601 gtagcattgc cgggtgagtg tattggagaa tcataatacc ccaaaggcaa agtgatggca
     9661 ttgccggtgc catacaccca tgttgccgaa agcgtaatgc catttttttc aggcttcact
     9721 tcacgtaagt gataaatggc aacaacagaa atatcgtgcc tgcgatcgaa tcgggcataa
     9781 aactttttgc caaaattgag ttcatcaaat tgtagttgtg tccacgaaag tgtgtatgca
     9841 atccatccgg taagtttgcc ttctttgcgt tgcaataata attcacctcc atacgaccag
     9901 cctttacccg atgtaacttt atcttcccag ttataatcgt tgtcaacctg at
//
LOCUS       DAORQA010000052         9860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1817_length_9860_cov_17.808261, whole genome shotgun sequence.
ACCESSION   DAORQA010000052 DAORQA010000000
VERSION     DAORQA010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9860
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1817_length_9860_cov_17.808261"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2305)
                     /locus_tag="PK736_04675"
     CDS             complement(<1..2305)
                     /locus_tag="PK736_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC32717.1"
                     /translation="MFKLKALFFVPLLFFYSASFAQFSPGAAGGATKVYDGKVSGTVI
                     DSVTQAAVEFATIALINKENNKPIDGAVTDENGNFKIKNIKNGNFKISVSFIGYTTVE
                     IDNIDITDKKKSFHFEKIIFAADGKYLKEAVVEGEQSLIENKIDKIVFNADKDITTKG
                     GNATDVLKKVPMVSVDLDGNVMLRGTTNIRVLINNKPSSIMAGSVADAMKMIPADNIE
                     KVEVITSPSAKYDAEGTGGIINIITKKKNIEGLSGSVNASAGTRSSSLFGNINYKKGR
                     FGVGSGFGGFMYRGLGNSEALRSSTTALSPFYLKQKGSNNNLGVGPSLSLNFDYDISS
                     RNNLSLTTKVNNFYNRSNTDVDNLVSFDNNEYTEIFRRQANNKTNSLGFDVNLDYKRS
                     FKNPSKEFTVSLQTIRSNRTTAYDALQYTPSDSLTLDETSENLNVNKEHTLQIDYVLP
                     VNKKIELEAGAKGILRNVNSDFDFNVFDFASQQYNYDSIRSNVFDYDQNVAAAYLQSS
                     FKIKDKYGIKAGARFEQTYVKGDNSIKDNKIFRNDYYNVIPSVTVSYTNKKRNTLKLS
                     YTQRIQRPSMQFLNPYVNDSDPNNISYGNPTLEPELSHAMEMGYNFFKGFSSINTSLY
                     HRRTNNSIENIRFINANNILVSTYDNIGKNTSTGMSLGGNYMYKMKFMIGGNFNVFYY
                     TVNSNTENFKLKNDGINYNINFFSSYTFTNRWGVQGFGNFNGPKYTVQGKSTSFFFYS
                     VGARREFKSKKGGVSFGLDNFASPIMNF"
     gene            2431..3144
                     /gene="rnc"
                     /locus_tag="PK736_04680"
     CDS             2431..3144
                     /gene="rnc"
                     /locus_tag="PK736_04680"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRC32718.1"
                     /translation="MNWLRLLGINFSEDPEFAKSLSNVIGFYPGNISVYKLAFCHKAV
                     AKENLQGFKMSNERLEFLGDAIVGAVIADLLFKKFPYREEGFLTEMRSRIVSRESLKK
                     LAVKMGIDKFLEENEFASKSMFGDAFEALVGAIYIDKGYAKAKSFLINRVVNIHLDLE
                     ELENNDTNYKSRILNHSQKEKHSLEFELVSENTVTKQFTIRIMYNQKEISRAVDYSKK
                     RAEQQAAAIACKKLGILET"
     gene            complement(3147..5981)
                     /locus_tag="PK736_04685"
     CDS             complement(3147..5981)
                     /locus_tag="PK736_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC32719.1"
                     /translation="MRYLIFLFCFSPLFLFGQLQTINGNVQNTNNEALPGCIIQLLRN
                     ADSLMIKNTVTDANGKFEIPGIAINKSYILKTTLLGYKTNYLAVNINTTKPAELKITL
                     KISAKEIATINVEGKALAVTIKGDTTEINSDSYKVNKDASGEDLVTKMPGVQVQDGKI
                     QAQGEDVKQVLVDGKAFFGEDPNAVLKNIPAEVIERIQVFDKRTDQSSFTGFDDGNSS
                     KTINIITRPQFRNGTFGRVYAGAGTDDRYKIGGVINKFKDNKRFTIIGQSNNINEQNF
                     TQDDLSGVLSSTGGSGRGGMGGGRGGMGGGGRPGGMGGGRGGTGQGGDGSQFIIDPKN
                     GITTTHAIGINYTDKWKTKTDVSGSYFFNFNKNIFDANTQRIYATTQNENLVYNEQTN
                     STSENMMHRFNFKVEHKFDSLHSYIIQPRIAFQNSNTNNSTLGLNTATTKTTGTTSNT
                     QVGENLNYNASVMMLYRHAFIKRGRTFSIGATPTISNGNSENILKSNNTVQLADTNFN
                     TSLDQKSTLDKNALNINANINYTEPLDTNHSLYWGYTLATQQNDAVKETYNYGPIKKD
                     YVIFNPLLSNTTNSDYIANGGEMGYRFQKSKWNISAGVNYQYATLKNKNATGATTDFN
                     KSFTNILPNAMLMYRWNTRQNMRFNYRTYTNMPGVEQLQPVLNNSNPLFLTIGNIDLN
                     QDLQHNLMLRYSSIDPLKNTSLFWLGGFTFINNYISNQTFIGKNDSNITTVNLPPGAQ
                     LSQPINVNGYYNARTFLSYGFPVKFLKSNLNINASANYTSTPAKQNNKMNITTSPSAS
                     LGLVLSSNISDKVDFTISSTSSGNAVRNDIDSRLNQDFYRQISKVRINVVVWKNINLS
                     SEATHNYNKGLGSSFNESFLIWNAGAGYKFLKNNIAEIRFSVFDILNENRNIQRNVTE
                     TYLEDVRSNALQRFFMLTFTYKISKTFAKK"
     assembly_gap    6574..6673
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6977..7702
                     /locus_tag="PK736_04690"
     CDS             6977..7702
                     /locus_tag="PK736_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32720.1"
                     /translation="MKAIISTAVTMLILSNANAQAPQFLSNTKIQPEFKTMVVETFKP
                     LKFETKGENVIWNFGKTKFKANGDEVYVVNPKDVNINKSFPDATHVKKVMNGGESIVY
                     EFLKIQGDKYEYLGNVVTDQSFNVTKYSDTQTILKLPFSFGETFNDVFESNVGNPLIV
                     TTSYDAYGTLILPFGTYTNVALVKCVYDYGVTEYKWYQTGPYIRLLATQVEHPDYTYT
                     VAFRNELSPAANSDLTNWLGDNK"
     gene            7859..8707
                     /locus_tag="PK736_04695"
     CDS             7859..8707
                     /locus_tag="PK736_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32721.1"
                     /translation="MKKIFLLIALIFLGFITSYSQSANESCHCAGMTIKGKGTFYITA
                     GYNMDWFTKSDMHFKDTKTASYDFTIYDVKARDRSGLNKDFFRTNILIPQYSFRIGYW
                     FNNNKNTGIEVNYDHAKYVVINDQTVRMKGTINNEYFDKDTVLNPRFVSFEHTNGANY
                     CMANVMKRYTILNSKNEKHWLSAVAKGGIGFVLPRTESRIVGRHRNDRYHVAGYIAGV
                     DVGLRYDFARYFFLEQSGKGCFANYANVLLKQEGRARHHFFSIEYIFTLGFQFSAKWS
                     KNISAR"
     gene            complement(9126..9275)
                     /locus_tag="PK736_04700"
     CDS             complement(9126..9275)
                     /locus_tag="PK736_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32722.1"
                     /translation="MAEKFVALSLYFCGEIFWLKPNVKLFKPSDKSGGNDGLTLKPWC
                     IQWQK"
     gene            9447..>9860
                     /locus_tag="PK736_04705"
     CDS             9447..>9860
                     /locus_tag="PK736_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRC32723.1"
                     /translation="MEDKKEITLLGAGLIGSLAAVYLTKQGYTVTIYERRPDMRKETI
                     SAGRSINLALSDRGWRGLAGAGVDTLIRDNAIPMYSRHIHNLDGSEINQAYGKENQAI
                     YSISRRDINCLLMDAAEQNGAKIFFNHRVTNYDINN"
BASE COUNT         2951 a         1859 c         1833 g         3117 t
ORIGIN      
        1 taaaattcat gatgggcgaa gcaaaattat cgagcccaaa actcacacca ccttttttgc
       61 ttttaaattc tctgcgtgcg ccaacactat aaaagaaaaa cgatgtcgac tttccctgca
      121 cagtgtattt aggtccgttg aaatttccaa acccttgcac gccccaacga ttggtaaacg
      181 tatagctcga aaagaaattt atattgtagt tgattccatc atttttcaat ttgaaatttt
      241 cggtattgct gttcaccgta taataaaaca cattgaaatt gccaccaatc ataaatttca
      301 ttttgtacat atagttacca cctagactca ttccggtgga agtgttctta cctatgttat
      361 cataagtaga aaccagaata ttatttgcat ttataaagcg gatgttttcg atagaattat
      421 ttgtgcgcct gtgatacagc gatgtgttga tagacgaaaa tcctttaaag aaattatacc
      481 ccatttccat agcatggctc aactccggct ccagtgttgg gtttccgtat gatatgttgt
      541 tggggtcgct atcgttaaca tatggattta aaaactgcat gcttggccgt tgaatacgtt
      601 gtgtgtaact cagctttaag gtgttgcgct ttttattagt gtacgaaact gtaacgctgg
      661 gtatcacatt atagtaatcg tttctgaaaa tcttgttatc ctttatgctg ttatctcctt
      721 tcacataagt ttgttcaaaa cgtgctcctg cctttatgcc atacttgtct tttattttaa
      781 atgatgattg caaataggcc gctgccacat tttggtcata gtcgaaaaca ttgcttcgta
      841 tactgtcata gttatactgt tgcgatgcaa agtcgaaaac attaaaatcg aaatcgctgt
      901 ttacatttct tagtattcct ttggcaccgg cttccaattc tattttctta tttacgggca
      961 atacataatc aatttgtaaa gtatgttctt tattcacatt aaggttttcg cttgtttcat
     1021 caagcgtaag cgaatcggaa ggtgtgtatt gcaatgcatc gtatgcagta gtgcgattgc
     1081 tgcgaatggt ttgcaggcta acggtaaatt cttttgaagg atttttaaag cttcgtttgt
     1141 aatcgaggtt aacatcgaac ccaaggctat ttgttttgtt atttgcctgg cgtctgaata
     1201 tttctgtgta ctcattatta tcaaacgata ctaaattatc aacatcggta ttgctgcggt
     1261 tataaaaatt atttacttta gttgtaagcg ataaattatt tctgctgcta atatcataat
     1321 cgaaatttaa cgacaacgat ggccccacac caagattatt attgctgcct ttttgtttta
     1381 aataaaatgg tgacaatgca gtagtgcttg aacgcaatgc ttccgaattg ccaaggccac
     1441 gatacatgaa tccgccaaat ccactgccca caccaaagcg gcccttttta taattgatgt
     1501 tgccaaacaa actgctcgac cgtgtgcctg cacttgcatt taccgaaccg ctgagccctt
     1561 caatattttt tttctttgta ataatattga taattcctcc ggtgccttca gcatcatatt
     1621 tggccgatgg acttgtgatt acttccacct tttcgatatt atcggcaggt atcattttca
     1681 tagcatcggc tacactgcct gccattattg atgaaggttt attattgatt agcacacgaa
     1741 tgtttgtagt gccacgcagc attacattgc catcgaggtc tacacttacc attggtactt
     1801 ttttcagcac atcggtggca ttgccgcctt tggtcgtaat atctttatcg gcattaaaaa
     1861 ctattttgtc aatcttgttt tcaatcaaac tttgctcgcc ttccactacg gcttctttta
     1921 aatattttcc atcggcagcg aaaattattt tttcgaaatg gaaacttttt ttcttatcgg
     1981 ttatatcaat gttatcaatt tcaacggtag tgtatccaat aaatgaaacc gaaattttaa
     2041 aatttccatt cttaatgttt tttattttga agttgccgtt ttcatcggta accgcgccat
     2101 caatgggttt gttgttttct ttgttgatga gtgcaatggt agcaaactcc actgcagctt
     2161 gtgtgaccga gtcgataaca gttcccgaca ctttgccatc gtaaactttt gttgcgccac
     2221 ctgcagcacc gggcgaaaat tgtgcgaatg aggctgagta aaaaaatagc aacggcacaa
     2281 aaaacagggc ctttaatttg aacatataaa ttctatatta ttaatttttc aaaagtatga
     2341 aaacttttgt aggatgttgt gcgcaaaaat ttgttacacg tatataagaa atgttaaact
     2401 tgcagtttca aaatttaacg cagcttattt gtgaattggt taagattatt aggaattaat
     2461 ttttcggaag acccggagtt tgccaaatcg ttgagcaatg ttattggatt ttatcccggc
     2521 aacatatcag tgtacaagtt agcattttgc cataaagcag ttgcaaaaga aaatttgcag
     2581 ggcttcaaaa tgagtaacga acgattagag tttcttggcg atgcaatagt gggcgcagta
     2641 attgccgatt tgttattcaa aaaatttcct taccgcgaag aaggtttttt aaccgaaatg
     2701 cggtcgcgta ttgtgagccg cgaaagtttg aagaagcttg ctgtgaaaat gggcatcgat
     2761 aaatttttgg aagaaaatga gtttgcctcc aagagtatgt ttggcgatgc tttcgaagcg
     2821 cttgtgggtg ccatttatat tgataaaggt tacgcaaaag caaaatcgtt tttaataaat
     2881 cgtgttgtaa atattcacct tgatcttgaa gaattggaaa ataacgacac caattataag
     2941 agccgtattc tcaaccattc gcaaaaggaa aaacactcgc tcgagttcga attggtttcg
     3001 gaaaatacgg taaccaagca attcacaata cgtattatgt acaaccaaaa agaaatttcg
     3061 cgcgcagtag attacagtaa aaaacgtgcc gagcaacaag ccgcagccat tgcctgcaaa
     3121 aagttgggaa tactggaaac gtaattttat ttttttgcaa acgtcttcga aatcttgtaa
     3181 gtaaacgtaa gcatgaagaa tctttgcaag gcattgcttc gtacatcttc taaataggtt
     3241 tcagtaacgt tgcgttgtat attgcggttt tcattcaata tatcgaaaac agaaaagcgt
     3301 atttcggcaa tattattttt caaaaattta taacctgcac cggcattcca tatcaaaaag
     3361 ctttcgttaa aactacttcc caggcctttg ttataattgt gcgttgcttc gctgcttaag
     3421 tttatatttt tccacactac aacgttaatt cgcactttag aaatttggcg gtaaaagtcc
     3481 tgattcaatc tgctatcgat atcgttacgc acagcattgc ccgatgatgt agacgaaatt
     3541 gtaaaatcta ctttgtcgct tatgttactg ctaagcacaa gccccagcga tgccgatgga
     3601 gacgttgtga tattcatttt gttattctgc tttgccggag tgcttgtata gtttgccgat
     3661 gcgttaatat tcaaattcga tttcaaaaac tttacaggaa aaccatacga taaaaaagtg
     3721 cgggcattgt agtatccatt tacattaatg ggttgcgaaa gttgtgcacc gggcggtaag
     3781 ttaaccgtgg taatgttact gtcattcttg ccaataaaag tttgattact gatataatta
     3841 tttataaaag taaagccacc aagccaaaac agcgaagtgt tttttaaagg gtcgatagaa
     3901 gaataacgca acattaaatt atgttgtaag tcctgattca aatcgatatt gccgatggtg
     3961 aggaataatg gattgctatt attcaacacc ggttgcagtt gctccacacc gggcatgttt
     4021 gtataggtgc gataattgaa gcgcatattt tggcgtgtat tccatctata catcagcata
     4081 gcattgggca atatattagt aaacgattta ttaaaatctg tagttgcacc tgtggcattt
     4141 ttatttttta atgttgcgta ttggtaattt acccctgccg aaatattcca tttcgatttt
     4201 tgaaaacgat aacccatttc gccaccattt gcaatataat cgctgttggt ggtgttgctc
     4261 aaaagtggat tgaaaataac ataatctttt tttattggcc cgtaattgta ggtttctttt
     4321 accgcatcgt tttgctgtgt ggcaagtgta taaccccaat ataaagaatg atttgtgtcg
     4381 agcggttcgg tgtaatttat attcgcattt atgtttaatg cgtttttatc cagtgtactt
     4441 ttttggtcga gcgatgtatt aaaatttgtg tcggccaact gaacagtatt gttcgatttt
     4501 aaaatgtttt cgctgttgcc attcgaaatt gttggtgtag ccccaatgga aaaggtacgg
     4561 cctcgtttaa taaaagcatg acggtaaagc atcatcaccg aagcattgta atttaagttt
     4621 tcgccaactt gtgtattgct tgttgtgccg gtagtttttg tagttgcggt attcaatccc
     4681 aatgtactat tgttggtatt tgaattttga aatgcaatgc gcggttgaat aatatatgaa
     4741 tgcaacgaat cgaatttgtg ttctacttta aagttaaagc ggtgcatcat attttcgctc
     4801 gtagaatttg tttgttcgtt gtatacaaga ttttcgtttt gtgtagttgc ataaatgcgt
     4861 tgtgtgtttg catcaaaaat atttttattg aagttgaaaa aataacttcc gcttacatca
     4921 gtttttgttt tccatttatc ggtataattt attcctatgg catgtgttgt agtaattcca
     4981 tttttagggt cgattataaa ttgcgaacca tcgccacctt gccctgtgcc tccacggcct
     5041 ccgcccatac caccgggccg gccaccgcca cccattcctc cacgtccacc tcccatgcca
     5101 ccgcggccac taccacctgt tgaactcaat actcccgata aatcatcctg tgtaaaattt
     5161 tgctcgttaa tattattcga ctgccctatg atggtaaatc ttttattgtc tttaaattta
     5221 tttattactc cacctatttt gtatcggtca tcggttccgg cacctgcgta tacacgccca
     5281 aaagttccat tacgaaattg cggtcgtgtg ataatattta ttgtcttgct actattgcca
     5341 tcatcaaaac cggtgaacga tgattggtct gtgcgtttat caaatacttg tatacgttct
     5401 attacttcgg caggaatatt tttcagcacc gcatttgggt cctcaccaaa aaaagctttg
     5461 ccatctacca atacttgttt tacatcttcg ccttgtgctt gtatcttgcc atcttgcact
     5521 tgcactccgg gcatctttgt aacaagatct tcgccacttg catctttatt tactttatac
     5581 gagtcgctgt ttatttctgt ggtgtcgcct tttattgtta cagcaagtgc tttaccttct
     5641 acatttattg ttgcgatttc tttggcagat attttcaaag ttatttttaa ttctgctggt
     5701 tttgttgtat taatatttac tgccaaataa tttgttttat atccaagcaa tgtagtcttt
     5761 aaaatgtatg atttgtttat ggcaataccc ggtatttcaa atttaccgtt tgcatccgtc
     5821 actgtatttt ttatcatcaa tgaatcggca ttacgcaaca actgaattat acagccgggc
     5881 aatgcttcgt tgttagtgtt ttgtacgtta ccgtttatgg tttgtaactg accaaataaa
     5941 aacaatggag aaaaacaaaa gaggaaaatt aaatatcgca cgcagttaaa ttttatgatg
     6001 accgtttttg attcgcgagt gcgaatatag tttaaacata ataatttaag ttgttgagaa
     6061 aaaagcaatt caaaagggat aaggcaaaaa gcacgcagtg agtattctcg aactggcaaa
     6121 aaataattca aaaggcataa gtcgaaagaa aaatgcaatt caaaagggat aagtcaaaag
     6181 catgcagtga gtattctcga actggcaaaa aaacaattca gaagagttga gcaaaaagaa
     6241 taaagattta gtatggattc cttccgtcat tgcgaaggag gaacgatttt cctttcgtca
     6301 atgcgaaagc agaactcact tctttccgtc attgcgaagg aggaacgact gaagcaatct
     6361 ctgcatagca aagacttagc acagcagaga ttgcttcgga gttcgagaat acccactgtg
     6421 acaattcgca atgacgacaa taaccaatgt gcggcagaga tgcttcgcag ttcgagaata
     6481 ctcactgtga cactcagcat gacaattgca acagcactaa tgaccaatgc caatcctctc
     6541 aatgaccaat gccaatcctc acaatgacca atgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnntcctcac aatgaccaat gacagtcctc acaatgacca atgccaggca
     6721 gagatgcttc gttcctcagc atgacaaagt caacaaccaa ataactcaac aaccaaataa
     6781 ctcaacaacc aaataactaa ataaccaata accatcccca atgaccaatg acaatcctca
     6841 caatgaccaa tgcccacccc tacagtaaaa acccttattc gcatttcaca tgaaaccatg
     6901 cttgaagtgg cttgctgcat gtagtatgtt tgcctcagtt aattcaatac ataataacaa
     6961 ctaaaaaatt aaagttatga aagccattat cagcacagca gtaacaatgc taatcttatc
     7021 gaacgcgaac gctcaagcgc cacaattttt gagcaacact aaaatacaac ctgagtttaa
     7081 aactatggtt gttgaaacgt tcaagccttt aaagtttgaa acaaaggggg aaaatgtaat
     7141 ttggaatttt ggcaaaacaa agttcaaagc aaacggtgat gaagtgtatg ttgtaaatcc
     7201 taaggatgta aatataaata agtcgtttcc cgatgccaca catgtaaaaa aagtaatgaa
     7261 cggtggagag agtattgttt atgaattcct gaaaattcaa ggcgacaaat atgaatactt
     7321 aggcaatgta gttaccgacc aatcgttcaa tgttacaaag tatagcgaca cacaaaccat
     7381 tctaaagctg ccatttagtt ttggtgaaac attcaacgat gtgtttgaat ccaatgttgg
     7441 aaatccgctt atagtaacaa caagttacga tgcttatgga acactcattc tgccttttgg
     7501 aacttacaca aatgttgccc ttgtaaaatg tgtttacgat tatggtgtaa cagaatacaa
     7561 atggtatcaa accggccctt atattcgctt gcttgccaca caggttgaac atcccgatta
     7621 tacatacaca gtagcgtttc gtaacgaatt atcaccagcg gccaacagcg accttaccaa
     7681 ttggcttggc gacaataaat aaaagttaaa ctgcattact aattcaaagg cggcaacttc
     7741 ggttgccgcc tttttttatg cgataatttt ttaacagcat tgttttatca attcaaattt
     7801 atgaaatgta aaaatgataa tattgcatgt caaattttga taatgcgatt ttcaaaacat
     7861 gaaaaaaata ttccttctca ttgctcttat ttttttggga tttattacat catattctca
     7921 atctgcaaac gaatcatgtc attgtgccgg catgacaata aaaggcaaag gcacttttta
     7981 tattacggca gggtataata tggattggtt tacaaaaagc gatatgcatt ttaaagatac
     8041 caaaacagcc agttatgatt ttactattta cgatgtaaaa gcccgtgacc gttcgggatt
     8101 gaacaaagat tttttccgca caaatattct cattccacaa tattcttttc gcattggtta
     8161 ttggttcaac aataataaga ataccggcat cgaagtaaat tacgaccatg caaagtatgt
     8221 ggtaataaat gatcaaactg tacggatgaa aggcacaatc aataatgagt attttgataa
     8281 agatactgtg cttaatccac gttttgtttc gtttgagcat acaaatggtg caaattattg
     8341 catggcaaat gtgatgaagc gatacacaat actaaattca aaaaatgaaa aacattggtt
     8401 gagtgctgtt gccaaaggag gtattggttt tgtattgcca cgcaccgaaa gccgcattgt
     8461 tggccgccac cgcaacgata ggtatcacgt tgcaggatac attgcgggtg tagatgttgg
     8521 tttgcgttac gattttgcac gttacttttt tcttgaacaa tcgggcaaag gatgttttgc
     8581 aaattatgcc aatgtattgt tgaagcaaga aggtcgcgca aggcatcatt tcttttctat
     8641 agaatatatt ttcacgctag gttttcagtt ttcggcaaag tggagcaaaa atatttctgc
     8701 acgatagaaa tacaattcaa atgtttcttg ccacgcctgc cccgaccaca gtgtcgggga
     8761 atgcgcgaat gaataccgaa tattttattg acactaagac gcagaggcac aatggttttt
     8821 gcgcgagccc atcattgccg tcctatttat cggacggttt aaataatttg cattaggctt
     8881 tagccgaaat attttgccgc gcctgccccg accacagtgt cggggaatgc gcgaatgaat
     8941 gccgaatagt ttattgccac aaaaccacaa aggtttttgc gcaagccaat cattgccgtc
     9001 ctatttatcg gacggtttaa ataattcgca ttaggcttta gccgaaatat tttgcagcga
     9061 attcgcgaat aaatgccgaa ttcatttttg ccacaatggc gcaaaggcac aaagtttttt
     9121 ttaattcatt tttgccactg aatgcaccaa ggttttagcg taagcccatc attgccgccc
     9181 gatttatcgg acggtttaaa taattttaca ttaggcttta gccaaaatat ttctccgcaa
     9241 aaataaaggc tgagtgccac gaatttttct gccaaagtat ttcagggata tcaaatgtag
     9301 tggcggtacc caaaccatga gattttttca acaaattatt tgctttaaaa atttgaaatg
     9361 aaatataatc tcaccaattt cgaacgcgat aaaaattggg cattaaaaaa gatcgggcac
     9421 aatttttatt taaacaaaaa aacaaaatgg aagataaaaa agaaatcaca ttactcggtg
     9481 caggattaat aggttcactt gctgctgtat atttaaccaa gcaaggatat acggttacca
     9541 tatacgaacg cagacccgat atgcgcaaag aaacaatttc ggcagggcgc tctatcaatc
     9601 ttgctttgag cgatagaggc tggcgcggat tggctggtgc cggggtcgac accctcattc
     9661 gcgataatgc catacccatg tatagcaggc atattcacaa cttagatggc agcgaaataa
     9721 atcaggctta tgggaaagaa aaccaggcca tttattccat ctcgcgcagg gatataaatt
     9781 gcttgttgat ggatgcagcc gaacagaatg gcgcaaaaat atttttcaat caccgcgtaa
     9841 caaattacga tatcaacaat
//
LOCUS       DAORQA010000053         9787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1828_length_9787_cov_13.245993, whole genome shotgun sequence.
ACCESSION   DAORQA010000053 DAORQA010000000
VERSION     DAORQA010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9787
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1828_length_9787_cov_13.245993"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..2279
                     /locus_tag="PK736_04710"
     CDS             39..2279
                     /locus_tag="PK736_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC32724.1"
                     /translation="MRKFFTGAIACAITFFGANKIYAQSGEITGQVGDAANKEILVGV
                     TVIVDDTVGTSTNATGFYRLQVSEGQHKIAFRMLGYQEYKTVFTISAKQALMINAKLK
                     VAATDMGMVVVSASKFEQKLNEVVVSMNVVKPQLIENNNQTSLTGVMEQVPGVTIVDN
                     QANIRGGSGFSYGAGTRVLMLVDELPLLAADANDVKWSFMPIENIEQIEIIKGASSAL
                     FGSSALNGIINVRTAYPKSTPETKINFFAGMYDQPKDRRQRFWGKEDVQAVTGYNFLY
                     SQQIKNLDLVFGGMVNNDDSYREGDNEKRFRVNTNLRYRVKGLPGFSFGVNANYQRAQ
                     GSTFFIWRNDTTGGLLAADNTISDYNTQRFSIDPFVTYVSPHFGTHKLKFRNFNTRNY
                     NNTDQSSFANNNYLEYQYQQKFAELVTVTAGAVNQDALIRGEFFNRHTSTTTAFYLQT
                     DWKWKRFNFSLGGRMENNVMDTIKIDYKPVLRSGVNVELRKGTNLRASYGQGYRFPSV
                     AERYVKTNIGIVSVFPNDSLITESGWSAELGIQQGYKFGTTLGYIDVAVFQMEYTNMM
                     EFVYGVWSSTNIGFSSRNIGNTRITGVDITWFNQGKFGKIPLNIFAGYTNINPIKTDY
                     NPVEDTVSGTANFNILKYRYRQMLKADVETGYGKFLIGANVRYYSEMENIDRIFAVFI
                     AGVDNYQKTRTGGDWIADVRLIYNANARVKIGAVVKNVANRFYVNRPANPQALRFFSL
                     QVSCKF"
     gene            complement(2282..2782)
                     /locus_tag="PK736_04715"
     CDS             complement(2282..2782)
                     /locus_tag="PK736_04715"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRC32725.1"
                     /translation="MQKSTHYYDSPIGSMEICTQGNELISCVFLEDMKTGTFAADPFG
                     ALVLSQLDEYFKGTRKQFQVHLGDVGTLFDNLVWEEVGRVKFGYTASYQDIANGISAP
                     DSVRAVGASNGRNPILLFVPCHRVIATDGSLRGYAGQVWRKKWLLEHESRIEWGVTTL
                     FDIEKT"
     gene            complement(2808..3572)
                     /locus_tag="PK736_04720"
     CDS             complement(2808..3572)
                     /locus_tag="PK736_04720"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HRC32726.1"
                     /translation="MSIHKDLSEAWQSYNLAHKLIVKHKLWLYIIAPGIINVIVFFAT
                     YKLAMAGIDTIIDMTIKIIDMWSHSITWIKPLLGAITFTLELIFRFLFFIIYISIYKN
                     IILVFLSPVLALLSERTEKILTGKEYPFEFLQFIKDIGRGIKLALRNLFIEIFLWIVL
                     LIVAFIPILNLLSPLVIFITSSYFTGFSMMDYYNERKKFTMRMSINIINLHRWKAISN
                     GILFYALFAIPFVGWIISPSYAVVAATIVRHKMTIK"
     gene            3976..4398
                     /locus_tag="PK736_04725"
     CDS             3976..4398
                     /locus_tag="PK736_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC32727.1"
                     /translation="MRQKLNIITLGVKDLEKALEFYEHGLGWKRSVASQGDIVFFPLG
                     GIILALYPRHLLAEDITINDDGKGFAGITLAINTKSETETDDILTLVQSLGATILKPA
                     QKVFWGGYSGYFKDLDGHIFEVAFNPFVQFDDNDNLIM"
     gene            4478..6355
                     /locus_tag="PK736_04730"
     CDS             4478..6355
                     /locus_tag="PK736_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC32728.1"
                     /translation="MKKNTTVQSKPKQKSPQPKFSEWMYAIILFVIAFAFYANSISNG
                     FALDDLIVITANKFTQQGVAGIKDIMTHDAFVGTYGEALNLSGGRYRPLSIVSFAIEK
                     QVFGNNTSVYHFFNVLFYSALCALLFVVLRKLFAQQNIFIPFVASLLFTIHPIHTEVV
                     ANIKSRDEIYMLFFSLLTFYFLLKDFKWKIIAACITFFLALLSKENAITVLFWMPLAF
                     YLFKNESIKTIAIKFAPLIIVAAIYLFMRAEFAGMVGDRTTTAQIDDSYLHATFIQKL
                     ATIAWVQLKYIELLLIPYSLSWDYSFNQLPLVSFTNPVSIISLLIHLGAIIYAIIYLK
                     KKPVISFSILFYLAAFFLVSNLVFNVGTSMAERFAFLPSVPFCLLVAGVTAYLLKIDY
                     TTPMRLTATVYVALSLIIVVAAAKVIPRNKDWKDNFTLYAADVSKVPNSARSRLFYGI
                     ECIGMYQKTNDRKWIDDAIANCKLSSEIDTTFHFAYHNLGVAYQTIGDYTASIPCYEN
                     VLKYSKDNPQAYYGLGTAYGKGLQQYDKAKTYFVKLIDELKLTNPEYYEALGLCYALQ
                     GNGTQAIDILKRGIEKNPRGAKLYYNTAITYANMLRADSSEYYFNIASSLDPSMQRPK
                     R"
     gene            6446..6973
                     /locus_tag="PK736_04735"
     CDS             6446..6973
                     /locus_tag="PK736_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32729.1"
                     /translation="MEKSIIDIIFYLAAPAFVLAAVYFLIRQFLNREYRLRLIEVKRM
                     MVKETLPLRLQAYERMILFLERISPNLLLMRFSDTSATVRELQLHLIETIRSEYEHNL
                     TQQIYISPQTWNIIKNAKEEMVRLINTSADKINPMSSHIELGKAIFETMMRNEEFPTQ
                     RAIDLVKTEAGLLFG"
     gene            7257..>7978
                     /locus_tag="PK736_04740"
     CDS             7257..>7978
                     /locus_tag="PK736_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRC32730.1"
                     /translation="MQEHKTDSGIEIKPLYNTPVKENELPGKFPYTRGIQEDMYRGKL
                     WTMRQYAGFSTAEESNNRYKFLLAQGTTGLSVAFDLPTQIGYDSSHDLADGEVGKAGV
                     AIDTLRDIEILFDGISLEKITTSMTINATASILLSMYIALAKKQGADIKNISGTIQND
                     ILKEYAARGTYIYPPKPSMRIITDIFEYCSKEVPKWNTISISGYHIREAGSTAVQELA
                     FTLANGKAYLKEAIAKGLDINV"
     assembly_gap    7979..8032
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8033..8811
                     /locus_tag="PK736_04745"
     CDS             <8033..8811
                     /locus_tag="PK736_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRC32731.1"
                     /translation="EVAKFRAARRMWAHITKELGATDAKAQMLRFHTQTGGSTLTAQQ
                     PYNNVARVTLQALAAVMGGTQSLHTNGFDEALALPTEQAAQLALRTQQIIAHESGVTS
                     TADPLGGSYFVEALTNEVEALAWDYIKRIDEMGGAVAAIEAGFIQKEIASSAYAYQRA
                     IQSGEKIIVGQNKFTTDETHITQLLTVDDSIRKLQMDKIEKVKSERDNIAVVTALAQL
                     EQAARDGSNVMPHILHATESYATLGEMADVMRNVFGEYRS"
     gene            complement(8899..>9787)
                     /locus_tag="PK736_04750"
     CDS             complement(8899..>9787)
                     /locus_tag="PK736_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32732.1"
                     /translation="DYVGCDVGRNLGYCYNGDPFDEGSFGYGANPPVVGTCILQGPIA
                     PANDGIDNDHDGTIDEVCEQVLMSSFMFYHNVNNQPNGNPDGPIHHYNYLKATWGDGL
                     HCTYGNDGRNPANPICNYMFSDNTDPINFPLYGAWTEVTAGNAPEDRRYIQSAGPFDL
                     LPGQVSYTTFAALWFRDTTGATNAISGLQLLADSARIMFDNWCPYPAAVNELNVNISS
                     SVTPNPALDFITINCNQLTNQKYNVIIMDINGKVVKKIIGQQSDETLVDVKSFVPGVY
                     YYKITAADKNVSTGKFVKR"
BASE COUNT         3071 a         1773 c         1898 g         2991 t
ORIGIN      
        1 ctctcaaagg aaattcttca aaaaaatatt ttaggtgtat gagaaaattt tttacaggtg
       61 caattgcatg tgccataact ttttttggcg caaacaaaat atatgcgcaa agtggcgaaa
      121 ttactggtca ggtgggcgat gccgccaata aggaaatact tgttggcgta acggtaatag
      181 tagacgatac tgtgggcact tctacaaatg ctaccggttt ttatcgattg caggtaagcg
      241 aaggtcaaca taaaattgct ttccgcatgc ttggttatca agaatataaa acggttttta
      301 caataagcgc caagcaggca ttgatgataa atgcaaagtt gaaagttgcc gctaccgata
      361 tgggaatggt ggtagtgagt gcaagtaaat ttgagcaaaa gcttaatgag gttgttgtgt
      421 ccatgaacgt ggtgaagcca cagttaatag aaaacaataa tcaaacatct ctcaccggtg
      481 tgatggagca agtgcccggt gtaaccattg tcgacaatca ggctaatata cgcggtggca
      541 gtgggtttag ctacggtgct ggtacgcgtg tacttatgct ggtagatgaa ctgcctttgc
      601 ttgcagccga tgccaatgat gtgaaatgga gttttatgcc catcgaaaat attgagcaaa
      661 ttgaaattat taaaggtgca tcgagtgcac tgtttggatc atcagcattg aatggaataa
      721 ttaatgtgcg aacagcatat ccaaaatcaa cacccgaaac aaaaattaat ttttttgccg
      781 gcatgtacga tcagcccaaa gatcgcaggc aacgattttg gggtaaagaa gatgtacagg
      841 cagttaccgg ttataatttt ttgtacagtc agcaaataaa aaatctcgat ttggtttttg
      901 gcggcatggt caataatgat gacagctatc gcgaaggtga taacgaaaaa agatttcgtg
      961 taaacacaaa tctacgatac cgtgtgaaag gtttaccggg ctttagtttt ggcgttaatg
     1021 caaattatca gcgtgcgcag ggctccacat tttttatctg gcgcaacgat accacaggag
     1081 gactgctcgc tgccgataat acaatcagcg attacaacac acaacgcttt agcatcgatc
     1141 cttttgtaac atatgtgtcg ccacattttg gcacacacaa attgaaattt cgaaatttca
     1201 acacccgcaa ttataataac acggatcaat catcattcgc caacaataat tatttagagt
     1261 atcagtatca gcaaaaattt gccgaattag ttactgtaac tgccggtgct gtaaatcagg
     1321 atgcactaat acgtggcgaa ttttttaaca ggcatacatc aaccactacc gcattttatt
     1381 tgcaaaccga ctggaagtgg aaacgtttta atttttcgct tggcggtaga atggaaaata
     1441 atgtgatgga tactattaaa atagattaca aacccgtgtt gcgtagtggc gtgaatgtgg
     1501 agttacgtaa aggcacaaac ttacgtgcat catatgggca ggggtatcgt tttccatcgg
     1561 tagccgaacg ttatgtaaaa acaaatattg gaattgtgag cgtgtttccc aacgatagtt
     1621 tgattaccga atcgggatgg agtgcagagt tgggaataca acagggatat aaatttggca
     1681 ctacactagg ctatatagat gtggccgttt ttcaaatgga atatactaat atgatggagt
     1741 ttgtttatgg tgtgtggtca tcaaccaaca taggtttctc ttcgcgcaat attggtaaca
     1801 cacgcattac cggtgtcgat attacatggt tcaatcaggg taagtttggc aaaattcctt
     1861 tgaatatttt tgcaggttac acaaacatca atcctataaa aactgattat aatcctgtgg
     1921 aagatacagt atcgggtacg gcaaatttta atattttaaa gtatcggtat cgccaaatgt
     1981 tgaaagcgga tgttgaaacc ggatatggaa aatttttgat aggtgccaat gttcgttact
     2041 atagcgaaat ggaaaacatc gatcgcattt ttgctgtatt tattgcaggg gtagacaatt
     2101 accaaaaaac gcgcacaggt ggcgattgga ttgccgatgt gcgattaatt tataatgcca
     2161 atgcacgggt aaaaattggt gcggtggtaa aaaatgttgc caatcggttt tatgtaaaca
     2221 ggcctgcaaa tcctcaagca ttgcgcttct tcagcttaca ggtaagttgt aaattttagc
     2281 gttatgtttt ttcgatatcg aacaaagtag taacgcccca ctcaatgcgc gattcatgtt
     2341 cgagcaacca ttttttgcgc cacacttgac ctgcataacc ccgcaacgat ccatcggtag
     2401 ctataacgcg atggcacggc acaaataata aaataggatt gcggccattg gaagcaccca
     2461 ctgcgcgtac actatcaggt gcacttattc catttgctat gtcctgataa cttgccgtgt
     2521 aaccaaattt tacacgacct acttcttccc acactaaatt atcgaacaat gtgcccacat
     2581 cgccaaggtg aacctgaaac tgtttgcgtg tgcccttaaa gtactcgtca agttgcgata
     2641 gaaccaatgc accaaatgga tcggcagcaa aggttcctgt tttcatatct tcgagaaata
     2701 cacatgatat taactcgttg ccttgagtgc atatttccat tgagccaatg ggcgaatcgt
     2761 agtagtgtgt gcttttttgc atttgcgaat atatattttt tctgttttta tttaatagtc
     2821 attttgtggc gtacaatagt tgctgccacc acagcataag atggtgatat tatccatccc
     2881 acaaatggaa ttgcaaatag tgcatagaat aaaatgccat ttgatattgc tttccatcgg
     2941 tgcaaattaa taatatttat gctcatgcgc atggtgaatt ttttacgttc gttataatag
     3001 tccatcatcg aaaacccggt gaagtatgat gatgtaataa aaataactag tggcgaaaga
     3061 aggtttaaaa ttggaataaa tgcaacaatc aacaaaacga tccacaaaaa tatttctata
     3121 aataaatttc gcaaggcaag ttttatgcct ctgccaatat cttttataaa ttgtaaaaat
     3181 tcaaatggat attcttttcc tgtcaatatt ttttcggtgc gctcagatag taatgccagc
     3241 acaggtgata aaaataccag aatgatattt ttataaatgg aaatataaat tatgaaaaat
     3301 aaaaatctaa aaattaattc taatgtaaat gttatggcac ccagtaacgg ttttatccag
     3361 gtaatcgaat ggctccacat atcaattatt tttattgtca tatctattat agtatctata
     3421 cctgccattg ccaacttata tgttgcaaaa aaaactatca catttattat tcccggagca
     3481 ataatgtaaa gccataattt gtgttttaca attagtttat gtgccaggtt atagctttgc
     3541 catgcctcac ttaaatcttt atgaattgac atgtggcaaa tgttgaaaaa aagtttcttt
     3601 gttttctgca gggaaaaata tttttcacaa tatgtttgtt tatatgtggc gaaatttttt
     3661 ggttgaaata gagttgaagg tgtttttttg acatataacc ttgatgatta tatgtggtaa
     3721 tactgtttaa tttataactg aatagtaatg cttcgaattc aaaacgtcac caaaacaagt
     3781 attacagtat agtaccctcc attatttacc acgtaattta tagccaaatt cgaattggtt
     3841 atttgagttg tatatttact aatatgaata tgtgtggata tttttagaaa ataatttaat
     3901 acaatacaaa attaattgct tgcaaataga ccaacaataa aattactttt gcttacaata
     3961 aaactgaaaa aaatggtgcg gcaaaaatta aatatcatca cactcggtgt aaaagattta
     4021 gaaaaggcgc ttgaatttta tgagcacggg ttaggttgga aaagaagtgt tgccagtcag
     4081 ggtgacattg ttttctttcc gttgggcggc attattttag cattgtaccc tcgtcatttg
     4141 cttgcagaag atattaccat aaatgacgat ggtaaaggtt ttgcaggtat cacattagcc
     4201 ataaatacca agagcgaaac cgaaaccgat gatatattaa ctttagtgca atccttgggg
     4261 gcaacaattt taaaacccgc acaaaaagtt ttttggggag gatacagcgg atattttaaa
     4321 gacctcgatg gacatatttt tgaagtagct tttaatccct ttgtacagtt tgatgataat
     4381 gataatctca ttatgtaaaa atggaaaaga tacaaaagca tttttttata acgcaataaa
     4441 ttaatctctg tttgtgatat aatcctgaca tttcgcaatg aaaaaaaaca ctactgtaca
     4501 gtcaaagcca aaacagaaat cgccacagcc taaatttagc gagtggatgt atgctataat
     4561 tttgtttgta attgcatttg ccttttatgc caacagcatt tcaaacggtt ttgcccttga
     4621 cgacctgatt gttattactg caaataaatt tacacaacag ggtgtagcag gtattaaaga
     4681 tattatgact catgatgcat ttgttggcac ttatggcgaa gccttgaacc tttcgggcgg
     4741 tcgctacaga ccattatcaa tagtttcatt cgctatcgaa aaacaagtgt ttggtaacaa
     4801 tacttcggtg tatcattttt tcaatgtttt attttatagt gcattgtgtg cattgttgtt
     4861 tgttgttttg cgaaaattat ttgcccaaca gaatattttt attccattcg ttgcttcgtt
     4921 attatttacc atacatccca tacacaccga agtagtagca aacattaaaa gccgcgatga
     4981 aatttatatg cttttctttt cgctcctgac tttttatttt ttgttgaaag attttaaatg
     5041 gaaaattatt gctgcatgca tcactttttt tcttgcatta ctttcaaagg aaaatgccat
     5101 taccgtttta ttttggatgc cactggcatt ttatttattt aaaaacgaaa gcataaaaac
     5161 catagccata aaatttgctc cattaattat tgtggctgca atttatttat ttatgcgggc
     5221 agaatttgca ggcatggttg gcgatcgtac taccaccgca caaattgacg attcttattt
     5281 gcacgcaaca ttcattcaaa aattggcaac tattgcctgg gtgcaattga aatacattga
     5341 gctgctttta attccttatt ctttgtcgtg ggattattct tttaatcaat tgcctttggt
     5401 ttcgtttaca aatccagtga gtattatctc tttgttgatt cacttagggg caattattta
     5461 tgctattatt tatttaaaga agaagcctgt aatatctttt agcattttgt tttatttggc
     5521 agcattcttt cttgtttcca atttagtatt taatgttgga acaagcatgg ccgagcggtt
     5581 tgccttttta ccttcggtgc cgttttgctt gcttgtggca ggggttactg cctatttatt
     5641 aaaaattgat tatactacac ctatgcgatt aactgcgact gtatacgtag cgctttcatt
     5701 gattatagta gttgccgctg ccaaagtaat tccacgcaac aaagactgga aagacaactt
     5761 tacgctctat gctgccgatg taagtaaagt gccgaacagt gcgcgctcgc gtttatttta
     5821 tggtatagaa tgtattggta tgtatcaaaa aacaaacgac cggaaatgga tagatgatgc
     5881 aatagcaaat tgtaaacttt caagtgagat agatactacc ttccattttg cctatcataa
     5941 tcttggcgtt gcctatcaaa caattggcga ttatactgct tccattccct gttacgaaaa
     6001 tgtgttgaaa tacagtaaag ataatccaca ggcatactat ggtttaggta cagcctatgg
     6061 caaaggattg cagcagtacg ataaagcaaa aacttatttt gtaaaactaa ttgacgagtt
     6121 gaaactcacc aatcccgaat attatgaagc actcggattg tgttatgccc tgcaaggaaa
     6181 tgggactcag gccatagata tacttaagcg aggtatcgaa aaaaatcccc ggggggcaaa
     6241 gctatattac aacaccgcaa ttacatatgc taatatgctg cgcgctgata gcagtgaata
     6301 ctatttcaac attgcaagtt cattagaccc aagtatgcaa aggcctaagc gataatgaag
     6361 ttagcccttg cccaaagtgg aagtaatatt ctttggcaaa aatcgtttta agtttattat
     6421 tatcgcgttc aaatattttt gatttatgga aaaaagtatc atcgacatta ttttttattt
     6481 agccgctccg gcatttgtgt tggcggcagt atattttctt attcgtcagt ttcttaacag
     6541 agaatacagg ttgcgattga tagaagtgaa gcgcatgatg gtgaaagaaa cattaccgct
     6601 aagattgcag gcttacgaac ggatgatatt atttctggaa cgtatttcac ccaatttatt
     6661 acttatgcgt tttagcgata ccagtgccac cgttcgcgaa cttcaattgc atcttatcga
     6721 aaccattcgt tcagaatacg aacataacct tacacagcaa atttatattt cgccccaaac
     6781 atggaatatt ataaagaacg caaaagaaga aatggtacga cttattaata cttctgccga
     6841 taaaattaat cccatgtcat cgcacatcga attgggcaaa gccatattcg aaaccatgat
     6901 gcgcaatgaa gaatttccta cacaacgtgc cattgatttg gtaaagaccg aagccggatt
     6961 gttatttggg taattaattt tttttgccac gaaaaacacg aaatttattt ttgtaagggc
     7021 attcatttga aagaaccgta tacttttggg ttcaatgtat tttgccataa aaaaaacgaa
     7081 attgtttttc actaaaattt cgaatcgcga aaaatgcttt tgacttttgt gcttcctttt
     7141 tttattttta acttctggct tttccttaac aatcgtaaat tatttctttt caatttttta
     7201 ttcggttact ttacattctc atttagcgat agtatctttt caatttttta aaagcaatgc
     7261 aagaacacaa aaccgattcg gggatagaaa taaaaccttt gtacaataca cctgtaaagg
     7321 aaaatgaact gccgggaaaa ttcccgtata caaggggcat acaggaagac atgtaccgcg
     7381 gcaagttatg gaccatgcgc cagtatgcag gctttagcac tgccgaagaa agcaataacc
     7441 gttataaatt tttgttggct cagggcacta caggtttgtc ggttgcattc gatttgccta
     7501 cacaaatagg ttacgatagc agccacgacc ttgccgatgg tgaagtggga aaagccggtg
     7561 tggcaattga tactctgcgc gatattgaaa ttttgtttga tggaatttcg ctcgaaaaga
     7621 ttaccacgtc aatgaccatc aatgcaacgg cttctatttt gttgagcatg tatattgcac
     7681 ttgctaaaaa acaaggtgcc gacataaaaa atatttcggg cactatacaa aatgatattt
     7741 tgaaagagta tgccgcacgg ggtacgtata tctatcctcc aaaacccagc atgcgaatca
     7801 ttaccgatat atttgaatat tgcagtaaag aagtacccaa gtggaatact atttctattt
     7861 cgggatacca catacgcgag gcgggaagca ctgccgtgca ggaattagcc tttacacttg
     7921 caaatggtaa agcatatttg aaagaggcca tagccaaagg tttagatata aatgtattnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaagaggtt
     8041 gcaaaatttc gtgcggcacg cagaatgtgg gctcatatta caaaagaact tggcgctacc
     8101 gatgccaaag cgcagatgtt acgttttcat acacaaacag gtggcagcac acttactgcg
     8161 caacaaccat acaacaatgt agcacgtgta acattacaag cacttgccgc tgtgatgggc
     8221 ggtacacaat cattacacac taatgggttt gacgaagcgc ttgcattacc aaccgaacaa
     8281 gcggcacagc ttgcattacg tactcaacaa attattgcgc acgaaagtgg tgtaacatca
     8341 actgccgatc cgcttggtgg ttcttatttt gtagaagcgc ttaccaacga agtagaagca
     8401 cttgcatggg attatataaa acgtattgat gaaatgggtg gtgccgttgc tgcaattgaa
     8461 gctggattta ttcaaaaaga aattgcctca tcggcatatg catatcaacg tgcgatacag
     8521 agtggcgaaa aaataattgt tggccaaaat aaatttacaa ccgatgaaac tcacattacg
     8581 caattgctaa cagtggacga cagcatacgt aaattgcaaa tggataaaat agaaaaggta
     8641 aaatcggaac gcgataatat tgctgttgta actgctcttg ctcaactcga acaagctgca
     8701 cgcgatggaa gtaacgtgat gccacacatt ttgcatgcta cagaaagcta tgcaacactt
     8761 ggtgaaatgg ccgatgtaat gcgcaatgtg tttggcgaat accgttcata aaaaaaagcg
     8821 aatgccttgg ttcgtctacc gacaaaccaa ggcaaatttc tcccgacaaa ccaaagaaaa
     8881 tgtcgccatg caaacaggtt aacgtttcac aaattttcct gtgctcacat ttttatcagc
     8941 agcggtaatt ttataatagt acacaccggg tacaaatgat tttacatcaa ccaatgtttc
     9001 atcactttgc tggccgataa tttttttcac caccttacca ttaatgtcca ttataattac
     9061 attatatttt tggtttgtaa gctggttgca attaatggta ataaaatcaa gtgcaggatt
     9121 tggtgtaaca ctcgatgaaa tatttacatt caattcattt accgcagcag ggtaggggca
     9181 ccagttatcg aacattattc gtgcagagtc ggcaagcaat tgtaaccccg aaatagcatt
     9241 tgttgcgcct gttgtatcgc gaaaccataa cgcagcaaat gttgtgtatg atacttgacc
     9301 tggtagtaaa tcaaatggac cggcagattg tatatacctg cgatcctcag gcgcattacc
     9361 cgcagtcact tctgtccagg caccataaag aggaaaattt attggatcgg tattgtcgct
     9421 aaacatataa ttgcatatag gatttgccgg attgcgacca tcattaccat aggtgcaatg
     9481 gagcccatca ccccacgtag cctttaaata attataatga tgtattggcc catcaggatt
     9541 tccatttggc tgattgttta cattgtgata aaacataaat gaagacatca gtacttgctc
     9601 acatacttca tcaatagtgc catcatgatc attgtcaatt ccgtcattag ccggtgcaat
     9661 aggaccttgc aaaatgcaag tgccaaccac aggaggattt gcaccatatc caaaagagcc
     9721 ttcatcaaat ggatcaccat tataacaata tccaagattg cgacctacat cacatcctac
     9781 ataatca
//
LOCUS       DAORQA010000054         9718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1842_length_9718_cov_8.255925, whole genome shotgun sequence.
ACCESSION   DAORQA010000054 DAORQA010000000
VERSION     DAORQA010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9718
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1842_length_9718_cov_8.255925"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            330..1151
                     /locus_tag="PK736_04755"
     CDS             330..1151
                     /locus_tag="PK736_04755"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013058746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRC32733.1"
                     /translation="MQQYLDLLQHVLDNGTRKEDRTGTGTISVFGYQMRFDLQQGFPL
                     LTTKKLHTKSIIHELLWFIAGDTNIKYLNDNGVNIWNEWADENGELGPVYGKQWRKWE
                     TKDGRVIDQLTNVIEIIKRNPDSRRLIVSAWNVADVDKMALPPCHTMFQFYVAPPNES
                     KGETLPRLSCQLYQRSADIFLGVPFNIASYAMLTMMVAQVCNMLPGTFVHTFGDAHIY
                     SNHLDQVHLQLTRIPHALPRLKINPEVKNIFDFKFSDFEIVDYNPHPSIKAPIAV"
     gene            1158..1661
                     /locus_tag="PK736_04760"
     CDS             1158..1661
                     /locus_tag="PK736_04760"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRC32734.1"
                     /translation="MYTHHSNISLVVAVSKNLAIGKNNALLWKLSDDLKLFKKITSNA
                     TVIMGRKTFDSIGKPLPNRRNIIISRNQDFEASGCEVFDTLAHALEACHTDKEVFVVG
                     GAQIYNVALPLAHKIYITKVDATFDDADTFFPAFNMNNWNIITEQKYSANEKNEYDFT
                     FYELERK"
     gene            1658..1984
                     /locus_tag="PK736_04765"
     CDS             1658..1984
                     /locus_tag="PK736_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRC32735.1"
                     /translation="MSLNNTQRIVDEWIKTNGVRYFNELTNMALLSEEVGELARIIAR
                     KYGEQSFKENESKDSLADEIADVLFVLVCIANQTGVNLQEAFENNMAKKTNRDKDRHR
                     NNNKLK"
     gene            2029..3030
                     /locus_tag="PK736_04770"
     CDS             2029..3030
                     /locus_tag="PK736_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC32736.1"
                     /translation="MKNLHGLNKTHQLLFLIIAMLCFCSCNNNQKTKNVSLIEIGFID
                     AFEDATLAKARNGFVDALQQNGFKNDSNIHIKFLNAQGDIPMLTQACQSMLSGNVKFI
                     ATNSTMATIAAVKQTKDVPIFMMVAPRPDIAGLTKADGKSPSNLFGVYETLFYIDSSI
                     SIIHQLKPDVKKLGLIYNQAETQSQDALEVVKKACANNNIALLSLPVNSSADSKIVTE
                     ALLSKGIDVFFALPDNVIFSSFETIAKSCEKSNVPIFTSEAGLVSRGAVASYGADFYH
                     WGFQSGEQAANFLKSNSTKNLQPEEVKLRIRVINKQKAEQYGIIIPAGFEVIDTVSK"
     gene            3037..3891
                     /locus_tag="PK736_04775"
     CDS             3037..3891
                     /locus_tag="PK736_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC32737.1"
                     /translation="MEAYISAIIQGLCFSSLALGIFISMRVLNLPDITTDGSFTLGAA
                     VTAVMLAQQIPVYFILPAVCICGAIAGSITGIIHTVLRVHVLLAGIIVMTALYSVNLI
                     VMGRTNIPFDEGFFMSNIFGDSNIWITMTIYVAVLVVIINRLLLSDFGIVLRALGSNS
                     TMLTANGVNTKALTIKGLAISNALTALSGSMIAQHQQFADLNMGIGIVIIGLGSVIIG
                     ESLFSFFKSQVLFLKLVAVVFGALVFRVLIAFVLSQDVNPNYLRLITATVVLAVVALS
                     YKKNPAID"
     gene            3884..4642
                     /locus_tag="PK736_04780"
     CDS             3884..4642
                     /locus_tag="PK736_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC32738.1"
                     /translation="MINIENISVTYNAGTANEVKALQQLSLQVLPKSFVVLLGSNGSG
                     KSTFFNALSGTAQIDSGKIFIDNQPIHEWNAYKRSVLFTRVFQNPLQGTVTGLSVIEN
                     FRIAALRTHSSKNIFAGVSTSFRKKVQEVISESGLPLANRLDTLMGNLSGGQRQALTL
                     LMATMADCKVIMMDEPTAALDPRSAELVINTTQKIFQNSPVTIFYITHNMKEAVSLGN
                     RLLIFKEGRIAIDASDTEKQKWDLQRLHGWFDEG"
     gene            4865..5296
                     /locus_tag="PK736_04785"
     CDS             4865..5296
                     /locus_tag="PK736_04785"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRC32739.1"
                     /translation="MKINILPTILYSILIFGCAFAKMPKQNFLFIYKLEHYRQVSIIA
                     EQMPEFVGGVDSLKSFMKNNFSYSNAARANKVQGVIYIGFVVEADGTLSNIKILRGIK
                     NDYGLNAEAIRVVEIMPKWIPGKAAGKNVSINFVLPFQCKS"
     gene            complement(5306..6979)
                     /locus_tag="PK736_04790"
     CDS             complement(5306..6979)
                     /locus_tag="PK736_04790"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRC32740.1"
                     /translation="MKIALAQLNYHIANFESNTKKIIDVIKNCKRDGCDLVVFSEMNV
                     CGYPALDFLEFDDFISRCLDSVQHIAEHCVGIAAIVGGPSRNPILDGKNLFNSAFFVA
                     DGEVQHTVHKALLPTYDVFDEYRYFEPAKEFKCFDYKGFKLAITVCEDLWNLEDDPLY
                     TYCPMDELTKEQPHLMINIAASPFNYMHAETRQQILRKNVTKYGVPLIYVNQVGANTD
                     LIFDGGSLVMDSSGKNILKTQYFEEDIKYIVCEKNEASISLQPTHPQSEIALNVFNTE
                     AVQIERIHSSLILGIRDYFAKMGFSKAVFGMSGGIDSALVYYLSVMALGKENILPVMM
                     PSQFSSDGSVSDSQMMINRLGTKSHIIPIKEIFEHYEKTLQPIFNNAPFNVAEENIQA
                     RIRGTLLMAISNKQGYILLNTSNKSELAVGYGTLYGDMCGGLAVLGDLYKTKIYELAK
                     YINRNEEIIPNNIIYKAPSAELRPNQKDSDSLPEYDVLDAVLMQYIENRKSIQEIIAM
                     GFDEALVSRILRMVNNNEYKRYQFAPVLRVSPHAFGLGRRMPLVAKYLG"
     gene            complement(7099..7740)
                     /locus_tag="PK736_04795"
     CDS             complement(7099..7740)
                     /locus_tag="PK736_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRC32741.1"
                     /translation="MKKIILSLFICVLASQTYAQKFRFGLTATPLMIDWLKSNTDSLT
                     SGGIKLGFTYGLSAEFNLTDRYSVATGLHVMYRGGILEQAVPDGLGGAVTAHSNITAQ
                     YVELPVTLKMKTNAIGMFTYFGQFGLGLGYNIKGKSVTEFGTVTTTDKDFNKELVGMH
                     VALHIGLGAEYHITESTDIVGTIYWNNGFTDILKDKDYKAVTNNIGLSIGVLF"
     gene            7819..8823
                     /locus_tag="PK736_04800"
     CDS             7819..8823
                     /locus_tag="PK736_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32742.1"
                     /translation="MKLIKYFLIIFCFTGIFSCTHNPLEVDVSNIDAKLSFKNFETDL
                     LNADHIDSMVIKKLRIDYGIFFDVFCSRMLRLPANNDTIVIRELNRFKNDADIRDIYA
                     EVNKVFSDKNEMQKELLDFVKHYKYYFPGKVEPHFVTYISAFNYANITTDSIVGIGLD
                     FYLGSDCKFYPSLQFPQYVTEKLNKENIAVNCIGAWYQKLWEQDVTKNVCLDYMIYEG
                     KKLYFTDAMSPKLSNAIKIGYTKEQWKWAEKYEKDNWAAFIENKLLFDNDYKKFIKLV
                     SDGPNSPGFALESAPKLGAYFGWKIVKAYMEKNKSITLSQLMMEQDAQKILEMSKYKP
                     "
     gene            9045..9371
                     /gene="gldC"
                     /locus_tag="PK736_04805"
     CDS             9045..9371
                     /gene="gldC"
                     /locus_tag="PK736_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HRC32743.1"
                     /translation="MKQSSIHFDISLSAEQMPSNIEWSASDSGIEGTKKAKAMLLSVW
                     DEKDPGTLRIDLWTDKMMVEEMQQFIYETLQSMGDTYQRATGDSEGAAEIKTMSTAFA
                     KKVKLI"
     gene            complement(9379..>9718)
                     /locus_tag="PK736_04810"
     CDS             complement(9379..>9718)
                     /locus_tag="PK736_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRC32744.1"
                     /translation="ETFWDGVRNYAARIHLNSMKVGDKVMFYHGNEGLAVMGIAKVTK
                     AAFPDPTSNDPQWVAVNIAPVKAFKRPVALAEIKAHPALQNIALVRIGRLSVMPLMKE
                     EFEIIEALGK"
BASE COUNT         3070 a         1771 c         1882 g         2995 t
ORIGIN      
        1 ttcattgtgt ttatgtttat ggccacaaat taaatatttt tttgcaagtt gtccatagaa
       61 taaaatgctt atagaattga gatgtaaatg aaacgaataa gtttaaaaaa tgagtccatt
      121 ccgaaaagtt atttttgcca caaaatcact aagccacaaa atttcactca gtcgtaatca
      181 aatcacttta atgttttgtg ggatttggtg atttagaggc tctttaattt atttgacttt
      241 tcggaaagga ctcaagaatc ttgttattaa aaaatgaaaa ataaaaatcg tacgttgcga
      301 ctgaaaaatt atttttacag aaaaaaatta tgcagcaata tttggactta ctgcaacatg
      361 tgctagacaa cggtaccagg aaagaagacc gtaccggcac cggcaccatc agtgtttttg
      421 gataccaaat gcgtttcgat ttgcagcaag gctttccttt actcactaca aagaaactgc
      481 acaccaaatc tatcattcac gaacttcttt ggtttattgc cggtgatact aatattaaat
      541 acctgaatga taatggtgta aatatttgga atgaatgggc cgatgaaaac ggtgagctcg
      601 gtcctgtgta tggaaagcaa tggcgcaaat gggaaactaa agacggacgg gtgattgatc
      661 aattgaccaa tgtgatagaa ataataaagc gcaatcccga ttcgcgcaga cttatagtaa
      721 gtgcatggaa tgtggccgat gtagataaaa tggccttgcc tccttgtcat accatgtttc
      781 aattttatgt ggcgccacca aacgaatcta aaggtgaaac cctgccacgt ttgagttgcc
      841 agctatatca gcgcagtgcc gatatttttc ttggtgttcc attcaatatt gcttcttatg
      901 caatgctaac tatgatggtg gcacaggttt gcaatatgtt gcctggtacg tttgtgcata
      961 catttggcga tgcacatata tacagcaatc atttggatca ggtgcatttg caacttaccc
     1021 gcataccaca tgctttgcca cgattgaaaa ttaatcccga agtaaaaaat attttcgatt
     1081 tcaaattttc cgacttcgaa attgttgatt acaatcctca tccttctata aaagcaccca
     1141 tagcagttta acggtttatg tatacgcacc attcaaatat ttctcttgtt gtagcggttt
     1201 cgaaaaacct ggctattgga aaaaataatg ccctgttgtg gaaactgtcg gacgacttga
     1261 agttattcaa aaaaataact tcgaacgcta cagttatcat gggccgtaaa acgttcgatt
     1321 ccattggaaa gccattgcca aacaggcgca acattattat ttcgcgcaat caagattttg
     1381 aagcatcggg ttgcgaagtt ttcgacacac ttgcacatgc tctcgaagct tgccatacag
     1441 acaaagaagt atttgtagta ggaggtgcac aaatttataa tgtggctttg cctctggctc
     1501 acaaaattta tatcacaaaa gttgatgcca catttgacga tgccgatact tttttcccgg
     1561 ctttcaatat gaataactgg aatataatta cggagcagaa atattcggca aacgaaaaaa
     1621 acgaatacga ttttacattt tatgaattgg aaagaaaatg agcctgaata atacacaacg
     1681 tatagttgat gaatggatta aaacaaatgg tgttcgttat tttaatgagc ttaccaatat
     1741 ggcattgcta agcgaagagg taggtgaact tgcacgtatt atagcccgta aatacggtga
     1801 acagtcattc aaagaaaacg aaagcaaaga tagtttggcc gatgaaattg ccgatgtatt
     1861 atttgttttg gtgtgcattg ccaaccaaac cggtgttaac ttgcaagagg cttttgaaaa
     1921 taatatggcc aaaaaaacaa accgtgataa agacagacac cggaacaaca ataaactcaa
     1981 ataggttttg tttgttgtaa acgcactcca caataacaca gattgattat gaaaaatttg
     2041 catgggctta ataaaacaca tcaattactt tttttaataa ttgcaatgct atgtttttgc
     2101 tcgtgtaaca ataatcaaaa aacaaaaaat gtttccctta tcgaaatagg atttattgat
     2161 gcctttgaag atgccacatt agccaaagcg cgcaatggtt ttgtagatgc attgcaacaa
     2221 aatggattta aaaatgatag caatatccac atcaagtttc tcaatgcaca gggcgatatt
     2281 ccaatgctta cgcaggcttg ccagtcgatg ttaagtggca atgtaaaatt tattgctacc
     2341 aattctacca tggcgactat tgcagcagta aagcaaacaa aggatgttcc catttttatg
     2401 atggtggcac caaggcccga tattgcagga cttacgaaag ccgatggaaa gtcaccctca
     2461 aatctgtttg gagtttacga aacattattt tacatcgatt cgtccatttc gatcatacat
     2521 caattaaaac ctgatgtaaa gaaattggga ttgatttata accaggccga aacgcaatcg
     2581 caagatgcat tagaagttgt aaaaaaagca tgtgccaata acaacatagc gctgctcagt
     2641 ttacctgtaa atagctcggc cgattcgaaa atagttaccg aagcattact ttcaaaaggt
     2701 attgatgtgt tttttgcttt gcccgataat gtaatttttt cctcgttcga aaccattgca
     2761 aagagttgcg aaaaatctaa tgtccctatt ttcaccagtg aggcaggatt agtaagtcgc
     2821 ggtgctgttg cctcgtatgg tgccgatttt tatcattggg gttttcaatc gggagagcag
     2881 gctgcaaatt ttttaaaaag caacagcact aaaaatttgc aacccgaaga agtgaaactg
     2941 cgcatacgtg taatcaataa gcaaaaggca gaacaatatg gaataattat tcccgcagga
     3001 tttgaagtga ttgataccgt atcaaaataa agaattatgg aggcttacat ttctgccatc
     3061 attcagggtt tatgtttttc atcgttagcc ttggggattt ttatttcgat gcgggtgctc
     3121 aaccttcccg atattactac tgatgggtca tttacgcttg gcgctgcagt tacagctgta
     3181 atgttggctc agcaaattcc tgtttatttt attttgccgg ctgtgtgcat atgtggtgca
     3241 attgccggca gtattaccgg tataatccat accgtattgc gtgtacatgt tttactcgca
     3301 ggcattattg ttatgacagc cttatattca gtcaacctca tcgttatggg ccgcacaaat
     3361 attcctttcg atgaggggtt ttttatgtcg aacatttttg gagatagcaa catttggata
     3421 accatgacta tatacgtagc ggttttagtg gttattatca accgcctgct actttccgat
     3481 tttggaattg tgcttcgtgc tttaggatct aattctacca tgcttactgc aaatggagta
     3541 aacacaaaag cgcttactat aaaaggattg gcaatatcca atgcgcttac cgcccttagt
     3601 ggtagcatga tagcacagca tcagcaattt gccgatctca atatgggaat tggcattgta
     3661 ataattggtt tgggaagtgt aattattggc gaatcgttgt tttcattttt caaaagtcag
     3721 gtgctatttt taaaattggt agctgttgtt tttggtgctt tggtatttcg agtattaatc
     3781 gcttttgtat tgtcgcaaga tgtaaatccc aattatttgc gactcattac agccacagtt
     3841 gtattagcag ttgttgcatt atcttacaaa aaaaatcctg ccattgatta atatcgaaaa
     3901 tatatcggta acctacaatg caggcacagc caatgaagtg aaagcattgc agcaattaag
     3961 tttgcaagta ttgccaaaaa gttttgtggt attgcttggc tcaaatggat cgggcaagtc
     4021 tacttttttt aatgcgctta gtggtacggc acaaattgat agtggaaaaa tatttatcga
     4081 taaccaaccc atacatgaat ggaatgcata taaacgtagt gtgctgttta ctcgtgtttt
     4141 tcaaaatcct ttgcagggaa cagttacggg attgagtgtg attgagaact ttcgcattgc
     4201 cgcattgcgc acacattcca gtaagaatat ttttgccgga gtaagtactt cgtttcgaaa
     4261 aaaagtgcag gaagtaatat ctgaaagcgg attgcccctt gcaaaccgac tcgatacatt
     4321 aatgggaaac cttagcggag ggcagcgaca ggcacttaca ttactgatgg caaccatggc
     4381 cgattgcaaa gtaattatga tggacgaacc cacagcagca ctcgacccac gctcggcaga
     4441 gttggtaatt aatacgacac aaaaaatatt tcaaaactcg ccagttacaa ttttttacat
     4501 cacacataat atgaaagaag cagtatcgct tggcaacaga cttttgattt ttaaagaggg
     4561 tcgaattgca atagatgcaa gcgatacgga aaagcaaaaa tgggatttgc aaagattgca
     4621 tggttggttt gatgaggggt gaaattgata gtcgcaagtt ttaattttga gtcctcgcag
     4681 aaaagtctag aaaggaaaat gccactaatt cacgaaaaca ctaaatccca caaacattac
     4741 aaatgatttg gttacgactt agtgaaattt tgtgtctttg tgcttttgtg gaaaaattgc
     4801 tttttggagt ctattcagta ttaaatgttt aaacgaaaag ggttaaagaa atttatatac
     4861 taaaatgaaa ataaatattt taccgacaat attatacagc atactaattt ttggctgcgc
     4921 atttgcaaag atgcctaaac aaaactttct ttttatttat aagttagaac actatcgtca
     4981 agtttcaatt attgccgaac agatgcctga attcgttggc ggtgttgatt ctttgaaatc
     5041 gttcatgaaa aataatttct cttactcaaa tgctgcgcgt gcaaacaaag ttcagggagt
     5101 aatatatatt gggtttgtgg tagaagctga tggaacatta tcaaatatta aaattctacg
     5161 tggaataaaa aatgactatg gtttaaatgc agaagcaata agagttgtgg agataatgcc
     5221 gaaatggatt ccaggaaaag cagcagggaa aaatgtgagt attaattttg tcttgccctt
     5281 tcaatgtaaa tcataatcca gaagcctacc ccaaatattt cgccaccaag ggcatgcgtc
     5341 tgccaagacc aaaggcatgt ggcgataccc ttaatacggg cgcaaactga taacgcttat
     5401 actcattgtt gtttaccatg cgtagtatgc gcgatacaag tgcttcgtca aagcccattg
     5461 caattatttc ctgaatgctt tttctgtttt caatgtattg catcaacacc gcatcaagta
     5521 catcgtactc aggcaatgag tcgctgtcct tttgattagg tctcagttca gctgaagggg
     5581 ctttgtatat tatattatta ggaataattt cttcgttgcg gtttatatat tttgccagct
     5641 cgtatatttt tgttttgtac aaatcgccca gcacggcaag gccaccgcac atatcaccat
     5701 acaaagttcc gtagccaact gccagttcac ttttgttact cgtgtttaat aaaatgtaac
     5761 cttgcttgtt cgaaatagcc attaataacg taccccgtat acgagcctga atattttctt
     5821 cggctacatt aaatggtgca ttgttaaaaa tgggctgcag tgttttttca tagtgctcga
     5881 atatttcttt tatggggata atgtgtgatt ttgtgcccag cctgtttatc atcatctgcg
     5941 aatcgcttac actgccatca ctcgaaaact gcgatggcat catcaccggc aaaatatttt
     6001 ctttgcccaa tgccataact gacaagtaat aaaccaaggc actatctatt ccgcccgaca
     6061 tgccaaatac agctttacta aaacccatct tggcaaaata atcgcgaatg ccaagtatta
     6121 acgatgaatg tatacgttct atctgaacag cttcggtgtt aaaaacattg agtgcaattt
     6181 cactttgtgg gtgagttggt tgcagtgaaa tagaagcttc atttttttcg catactatgt
     6241 attttatatc ctcttcaaag tattgagttt tcaatatatt tttccctgag ctgtccatca
     6301 caagcgaacc accatcaaaa atcaaatcgg tatttgcacc aacttgattc acataaatta
     6361 agggcacacc atatttagtt acattttttc gcagtatttg ttggcgggtt tcggcatgca
     6421 tataattgaa aggcgatgcg gcaatattta tcatcaaatg gggttgttct tttgtaagct
     6481 catccatagg gcaataagta tataaaggat catcttcaag gttccataaa tcttcgcaaa
     6541 cagtaatggc taatttaaaa cctttgtaat cgaagcattt aaattctttg gcaggttcaa
     6601 aatatcggta ctcatcaaat acatcgtagg tgggcaacag ggctttatga accgtatgtt
     6661 gcacctcgcc atcggcaaca aaaaatgcgg agttaaaaag atttttgcca tcgaggatag
     6721 gattgcgcga tgggccacca actatagccg ctattccaac acaatgttca gcaatatgtt
     6781 gcacactatc gagacaacga gaaatgaaat cgtcaaattc caaaaagtcc aatgccggat
     6841 atccgcaaac attcatttca gaaaaaacaa ccaaatcgca accatcgcgc ttacaatttt
     6901 taattacatc aattattttt tttgtattgc tttcgaaatt ggcaatatga taattcaatt
     6961 gtgccagtgc tattttcatc tttccttttg ttttactcaa tgcaaattca attgcaaaat
     7021 aaactttagt tattcatata aaaaaataaa acccgattca tggaaccggg tttcgcaact
     7081 taaatatctt ttatgatgtt aaaacaagac accaatgctg agtccaatat tatttgttac
     7141 cgctttataa tctttgtctt taagaatgtc ggtaaagcca ttgttccaat aaatagtgcc
     7201 aacaatgtct gtcgactctg taatatgata ctcggcaccc aagccaatat gcaaagcaac
     7261 atgcatgcct accagttctt tattaaaatc tttatcggtt gtagttactg ttccaaactc
     7321 ggtaacgctt ttacctttaa tattataccc caaaccaaga ccaaactgcc cgaagtaagt
     7381 gaacatgcca attgcatttg ttttcatttt taaggttaca ggcaattcaa cgtattgagc
     7441 agtaatgttg ctatgagcag ttacagcacc accaagtcca tcgggtacgg cttgctcaag
     7501 tatcccacca cggtacataa catgtaaacc tgttgcaaca gagtaacggt ccgttaaatt
     7561 aaattcggct gaaaggccgt aggtaaaccc aagttttata ccaccacttg taaggctgtc
     7621 ggtattagat tttagccagt caatcatgag tggagttgct gttaatccaa agcggaattt
     7681 ttgtgcataa gtttgacttg ccaacacaca aatgaatagt gataaaataa tttttttcat
     7741 attattaatt gtttatatta taaaataatt tttgattttt tgcattaaaa taagtggctg
     7801 caatgttaca catttaatat gaaactaata aaatattttc ttatcatatt ttgctttacg
     7861 ggcattttct cttgcacaca caaccctttg gaggttgacg tttccaatat tgatgccaaa
     7921 ttgagtttca aaaactttga gaccgattta ttaaatgctg atcacattga tagtatggtc
     7981 atcaagaaat tgcgcataga ttacggtatt tttttcgatg tgttttgttc acgcatgtta
     8041 cgtttgcctg caaataacga cactattgtg attcgcgagt tgaaccgctt taaaaacgat
     8101 gctgatattc gagatatcta tgccgaagta aacaaagtgt ttagtgataa aaatgaaatg
     8161 caaaaggagc tgctcgattt tgtgaagcat tataaatatt atttcccagg caaggtggag
     8221 ccacattttg ttacgtacat ttctgctttc aattatgcca acatcacaac agatagtata
     8281 gttggaatag gcttggattt ctatctgggc agcgattgta agttttatcc ttcgctgcag
     8341 tttccacaat atgttaccga aaaattgaac aaagaaaata ttgcagtgaa ttgtataggt
     8401 gcatggtatc agaaattgtg ggagcaagat gttacaaaaa acgtttgcct cgactatatg
     8461 atttatgaag ggaagaaatt atattttacc gatgccatgt caccaaaatt aagcaatgca
     8521 attaaaattg gttatactaa agaacagtgg aagtgggccg aaaaatatga gaaagataac
     8581 tgggcggctt tcatagaaaa taaattgttg ttcgataacg attataaaaa atttataaaa
     8641 cttgtgagtg atggtcctaa cagtcccggg tttgcactcg agtcggcacc taagcttggt
     8701 gcttatttcg gttggaagat tgtaaaagca tacatggaaa aaaacaaatc tataacgctg
     8761 tcacaactta tgatggaaca agatgctcaa aagattttgg aaatgtcgaa gtataaacca
     8821 tgattttttt tcggcacttt tttttgggca tatctttgtg gttgtggaga taattcttga
     8881 ctcgagttca agaaatatga ccaggaaaaa tatattggcg tatcaccttc ttaaaagtct
     8941 gccttccaaa ccaaaaaaca tttactatca taaaaatctg cattctgctt aactcaccta
     9001 aatgattttg tactattgca cacaaaatat tttgaaaaaa aattatgaaa cagtcatcaa
     9061 tacattttga tatatcgctt agtgcggagc aaatgcctag caatatagag tggagtgcaa
     9121 gcgactcggg aattgaagga acaaaaaagg caaaggctat gctgctatcg gtgtgggacg
     9181 aaaaagaccc cggcactttg cgtatcgatt tatggaccga taaaatgatg gtggaagaaa
     9241 tgcaacagtt tatttacgaa acattgcaaa gtatgggcga tacttatcag cgtgcaacag
     9301 gcgatagcga aggtgctgct gaaattaaaa ccatgagtac tgcttttgca aaaaaagtga
     9361 aactcattta atcggtttct atttccctaa agcttcaatt atttcaaatt cttctttcat
     9421 gagtggcata accgaaaggc gacctatgcg cacgagtgca atattttgta atgccggatg
     9481 tgcttttatt tcagctagtg ctacagggcg cttgaaagcc tttaccggag cgatatttac
     9541 agccacccat tgcgggtcat tggaagttgg gtcgggaaag gccgctttgg tcacctttgc
     9601 tatgcccata acggcaagcc cttcgttacc gtgataaaac attactttat cgcccacttt
     9661 catcgagttg agatgaatgc gtgcagcata attacgcacc ccatcccaaa atgtttca
//
LOCUS       DAORQA010000055         9634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1864_length_9634_cov_8.467481, whole genome shotgun sequence.
ACCESSION   DAORQA010000055 DAORQA010000000
VERSION     DAORQA010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9634
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1864_length_9634_cov_8.467481"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PK736_04815"
     CDS             <1..498
                     /locus_tag="PK736_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HRC32745.1"
                     /translation="SEIFSHGFSKFIEVPINGSYKDVQTIKVNNELAALNKIWAIGGK
                     TGWYYGNWLWELRGFIDQLTGGVGMRRGRKSDTEITAGDALDFWRVMLADKKEKRLLL
                     FAEMKLPGEAWLEFKIDDNHILTQTATFRPLGLLGRLYWYVVLPFHGFIFKGMINKIA
                     EEKST"
     gene            774..1415
                     /locus_tag="PK736_04820"
     CDS             774..1415
                     /locus_tag="PK736_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016958560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HRC32746.1"
                     /translation="MNLTYFTSKDILEMETRFRSAFINSLSGFKSASLIGSVDKDGNT
                     NLAIFSSVVHLGSNPALIGFINRPDATSRHTLENISATNCFTINHINETIYKPAHQTS
                     ARYPKNISEFDATRLTAEFGKVVKAPYVKESHVKYGLEFVEKHELKINGTILVIGKVV
                     EVFIPENCLLVHGAIAIEKAGTITISGLDSYHTTRQLAQLSYAKPDKMVEELL"
     gene            1430..2143
                     /locus_tag="PK736_04825"
     CDS             1430..2143
                     /locus_tag="PK736_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HRC32747.1"
                     /translation="MNTFLHAKWQNLIMANYEIDPSILFSYLPKGVELDYFNGKTYVS
                     LVGFLFKDSSIFNIPIPSMGTFEEVNLRFYVVRKVGDQLRRGVVFINETVPNKIVAWV
                     ANKLYKEHYIAIPTKHKWTFVNDKKEIEYQWKVQSKWNSIKVVATVAKQKMVAGSMEE
                     FIFEHYFGYTKVNSQHSIEYKINHPSWEINAIHHSHIDCDFAAFYGKDFELLNSIKPH
                     SIMLAEGSDISVDWKRISF"
     gene            2154..2291
                     /locus_tag="PK736_04830"
     CDS             2154..2291
                     /locus_tag="PK736_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HRC32748.1"
                     /translation="MKGVKKENLPTKICITCNKSFAWRKKWTKNWNDVKYCSDRCRNT
                     K"
     gene            2294..3844
                     /locus_tag="PK736_04835"
     CDS             2294..3844
                     /locus_tag="PK736_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HRC32749.1"
                     /translation="MAIKINNTLRLLLGDQLNINHSWFKTVDASVTYVLMEIRSETDY
                     ARHHIQKVIGFFAAMQNFAHELKLLNHQVIYISLNDKNNMQQFDKNCEALILKHKFTN
                     FEYQLPDEYRLDEHLKSFAGKLKITANVCDTEHFYSTRNELKDLFEGKKMYVMETFYR
                     YMRKKHQLLIIDGGKPLNGKWNFDENNRQKLPKAHQPTAPLLFANDVIEIEKEIIKAG
                     VKTIGTVDSKNFIWPINRTQSLALLDFFIENCLPLFGTFQDAMTPNEWSIYHSRLSFS
                     MNTKLLSPKEVVDKAIDAYFKNTDQIEYHQLEGFVRQIIGWREYMRGIYWMKMPEYAT
                     LNYFEHSEKLPQWFWTGKTKMNCLKNAIHQSLDYAYAHHIQRLMITGNFALLAGVHPD
                     EVDAWYLGIYIDAIEWVEITNTRGMSQFADGGIVGTKPYVSSATYINKMSSYCTGCYY
                     DKAKKTGDKACPFNSLYWNFYDKHEAKLARNPRIGMVYNVWKKMEPNAKAELLEQAEY
                     YLNHINEL"
     gene            3962..4252
                     /locus_tag="PK736_04840"
     CDS             3962..4252
                     /locus_tag="PK736_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HRC32750.1"
                     /translation="MKKQTEKFYFSEEDRDRIIQMAWEDRTPFEAIEFQFGLKEKDVI
                     EFMSKNSLPSSFRMWRKRMKARKTKHAALRDDGTNRFKCSRQRSTGNKISNR"
     gene            4556..5647
                     /locus_tag="PK736_04845"
     CDS             4556..5647
                     /locus_tag="PK736_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRC32751.1"
                     /translation="MNKNLNHQGKNFKVKHSAATIILLCIFTSAFLLESVNCSSDNRA
                     SKSERDSTLAEIAEMFTEEKLWTAPDIATIPNNDEGKLIKYGLNLVVHTSKYFGPKGS
                     VSSNGNGLNCQNCHMEAGTRPYGNNLGAASTIYPKYLPRSGTVVSLAEKINECFYRSL
                     NGDIIDTTSKEMRALVAYTTWLGKDFHKDPKLKGSRGIDAPHFIDRAADPAKGEKTYD
                     ELCARCHGKDGQGQFTEDVLKDITKQQGGIATADDLYYYPPLWGDKSYNAIATLYRVG
                     KLAGFIKHNMPYPLTYKNSVLTDEQAWDIAAYINSRERPEKDHSKDYSIDISKKPYDF
                     PFAPYADSYSQQQHKFGPFTDMPSAMKAH"
     gene            5653..6831
                     /locus_tag="PK736_04850"
     CDS             5653..6831
                     /locus_tag="PK736_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRC32752.1"
                     /translation="MRNYLQIFFACLLVFGCSQSGTNSDNEKGAEKETANAQAKKEMI
                     VLTDRPPNIETPLSYFKEDFTPNDVFFVRWHLSGVPASVDTNKFRLTISGHVKKELSL
                     SLEDLKTKFKATSIVALAECAGNARSFFDPRVPGGQWKNGAMGNARWTGVKVKDILDA
                     AGVKPGAFDVAFNGMDQPPLESVPDFIKSLRIDHAMDGEVMIAYEMNGAPLPLLNGYP
                     LKLVVPGWYATYWIGMLDEIKIYPDTFKGYWMQKAYLVPKGVRNGNEQPDSLAKVTEP
                     ISKLDVRSIFVSPEPDEVLSVNKKCVVEGLAFDAGDSIVKVEITTDGKTWQTANLDPV
                     IGKYSWRRWKFDWTPATAGTYTLSVKATNAIGETQPTHQWNRSGYMRNEIETLKLNVK
                     "
     gene            6873..7178
                     /locus_tag="PK736_04855"
     CDS             6873..7178
                     /locus_tag="PK736_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRC32753.1"
                     /translation="MCFVLFIASCTEEKSDKQKLTDKEKTRADIESMFTNAAGEEVYN
                     ANCITCHSLKYVDMQPPFPRKTWEKTVDKMIKNFGAPIPDSTAKVIVEYLMAVKGMN"
     gene            complement(7412..9610)
                     /gene="katG"
                     /locus_tag="PK736_04860"
     CDS             complement(7412..9610)
                     /gene="katG"
                     /locus_tag="PK736_04860"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HRC32754.1"
                     /translation="MNGEAKCPFHGGAVKQSAGAGPKNLDWWPNLLKLNILRQHSTLS
                     NPMGENFDYAEAFKSLDLAALKKDIFELMTQSQDWWPADYGHYGPLFIRMAWHSAGTY
                     RISDGRGGGGSGTQRFAPLNSWPDNANLDKARLLLWPIKQKYGNKISWADLMILAGNC
                     ALESMGFKTFGFAGGRADVWEPAEDIYWGPEGEWLGDKRYTGDRELENPLGAVQMGLI
                     YVNPQGPNGNPDPIAAARDIRETFGRMAMNDEETVALIAGGHTFGKSHGAADPGKYVG
                     VEPAAAAMEEQGLGWKNTLGTGSGVNTITSGLEGAWTTSPTKWSNNYFENLFGYEWEL
                     TKSPAGAHQWKPKGDAGAGTVPDAHDTNMRHAPMMLTTDLSLRLDPIYAPISKRFFEN
                     PDQFADAFARAWYKLTHRDMGPIARYLGPEVPKEELIWQDPIPAVNHKLIDGNDTAAL
                     KTKILASGLTVSQLVSTAWASAATFRGSDKRGGANGARIRLSPQKDWEVNNPAQLSKV
                     IGVLEGIQKEFNSAQSGGKMISLADLIVLAGCAGVEKAAHNAGIKTTVPFTPGRADTT
                     QEQTDVESFAVLEPGADGFRNYFKSKHTSKMEEMLVDKAQLLTLTAPEMTALVGGMRV
                     LNTNYNQSKHGVFTNNTEALTNDFFVNLLDFGTTWKPTTDAQDMFEGRNRATGELKWT
                     ATRADLIFGSNSELRAIAEVYGCADANEKFVNDFVAAWNKIMNLDRFDLK"
BASE COUNT         3064 a         1851 c         1932 g         2787 t
ORIGIN      
        1 agcgaaattt tcagtcatgg attttcaaag tttattgaag tgccaattaa tggaagctat
       61 aaagacgttc aaacgattaa ggttaataac gaacttgcag cattaaataa aatttgggcc
      121 attggtggta aaacaggttg gtattatggc aattggctat gggagttgag aggttttata
      181 gatcaactta caggaggtgt aggaatgcga agaggaagaa aaagcgacac ggaaataact
      241 gcaggtgatg cgcttgattt ttggcgtgtg atgttggctg ataaaaagga aaaaagatta
      301 ctcttatttg ctgaaatgaa attacccggt gaggcatggt tagaatttaa aattgatgac
      361 aatcatattc ttactcaaac ggcaaccttc cgtccacttg gcttgctagg caggctttat
      421 tggtatgttg tacttccttt tcatggtttc atttttaaag gtatgataaa taaaatagct
      481 gaagaaaaat caacataaat tcaaataaac agcgccccaa tcaatagcaa aaaaataatg
      541 gctgcatgat tgaataaata cttttgacga atacaaaact tcttcccggt tttagttttg
      601 tatttcgcac tcgctggctt ctttaaaatc tttatttgca tttcacattt tataggcaaa
      661 gatttatttg ctgcaaattt ctgcaatgaa aacattagat atcttttctg ctaagaagaa
      721 attaataact aatcgaaatc ttgtttgttt agatatcaat ttaacttaat agaatgaacc
      781 tgacctattt taccagtaaa gatattttgg aaatggaaac gcgcttcagg tccgcattta
      841 tcaattcgct aagtggtttt aaaagtgcat cacttattgg ctcggtcgac aaagatggaa
      901 atactaatct tgctattttt agttcagtgg ttcatctggg ttcaaaccct gcattaatcg
      961 gttttatcaa tagaccggat gcaacaagca ggcatacctt agaaaatatt tcagcaacaa
     1021 attgctttac tatcaatcat atcaatgaaa caatttataa accggcacat caaacctcgg
     1081 ctcggtatcc aaaaaatatt tctgagtttg atgcaacccg attgaccgct gaatttggca
     1141 aagtagtaaa agcgccttat gtaaaagaaa gccatgttaa atacggttta gaatttgttg
     1201 agaaacacga attgaaaata aacggtacca ttttagtcat aggtaaggtg gtagaagtat
     1261 tcatccctga aaactgtttg cttgttcatg gtgctatagc tattgagaag gccggaacca
     1321 taaccatatc cggtttggat agttatcata ctacacgtca acttgcgcaa ttatcttatg
     1381 ccaaacccga taaaatggtt gaagaattac tttaatcaat acatttagta tgaatacttt
     1441 tttacacgca aagtggcaaa atttaattat ggctaattat gaaattgacc cttcgatttt
     1501 attttcgtat ttgcctaaag gtgtggagct cgattacttt aatggaaaaa cgtatgtaag
     1561 tttggtaggc tttttattta aggatagttc tatttttaat atcccaatac cttctatggg
     1621 aacttttgaa gaagtaaatc ttcgttttta tgttgttaga aaagtgggag atcaattaag
     1681 gcgcggtgtt gtttttatca atgaaacggt gcccaataaa atagtggctt gggtggccaa
     1741 caaactttat aaggaacatt atatcgccat acccaccaaa cacaaatgga cttttgtcaa
     1801 tgataaaaag gaaattgaat atcagtggaa agtgcaatct aaatggaaca gcattaaagt
     1861 tgttgccact gttgcgaagc aaaaaatggt agcaggtagt atggaagaat ttatttttga
     1921 acactacttc ggttatacaa aagtaaatag ccaacacagt attgaatata aaataaatca
     1981 tcctagttgg gaaattaacg caattcacca ttcccatatt gattgcgatt ttgctgcttt
     2041 ttatggtaaa gatttcgagt tattaaacag catcaagccc cattccataa tgcttgcaga
     2101 aggttcggat attagtgtgg attggaaacg tattagtttt taaaacgata agtatgaaag
     2161 gcgttaaaaa agaaaatctt ccaaccaaaa tttgtatcac ctgcaacaag tcttttgcat
     2221 ggagaaaaaa atggacgaaa aactggaacg atgtaaaata ttgcagcgac cgatgcagga
     2281 atactaaata gacatggcca ttaaaataaa caatacttta cgcttactat taggtgatca
     2341 gctcaacatc aatcactcgt ggtttaaaac agtcgatgca tcggtaacct atgtgctaat
     2401 ggaaataaga agcgaaaccg attatgcacg acatcacatt caaaaagtaa tcggattttt
     2461 tgcagccatg caaaattttg cccatgaatt aaagctactg aaccatcaag taatttacat
     2521 cagtctgaat gataaaaata atatgcagca gtttgataaa aattgtgaag ccttaatttt
     2581 aaaacacaaa ttcaccaact ttgaatacca attacctgac gaataccgat tagatgagca
     2641 tttgaaaagc tttgcgggta aattaaaaat aacggctaat gtatgcgata cagaacattt
     2701 ttacagtact cgaaatgaat tgaaagattt gtttgaaggc aaaaaaatgt acgtgatgga
     2761 aaccttttat cgctacatgc gaaagaaaca tcagttacta attatagacg gaggcaaacc
     2821 tttgaatggc aaatggaact ttgacgaaaa caaccgtcag aaattaccaa aagcacatca
     2881 accaactgca cctttgctat ttgcaaatga tgtaattgaa attgaaaaag aaatcataaa
     2941 agccggagta aaaacaatcg gaactgttga cagcaaaaat tttatttggc ccataaacag
     3001 aacacagtca ttggccttac ttgatttttt tatagaaaat tgtttgcccc tttttggaac
     3061 ttttcaggat gcgatgactc ccaatgaatg gtctatttat cattcgcgtt tatccttttc
     3121 gatgaacaca aaattgcttt ctccgaagga agtggttgac aaagcgatag atgcttattt
     3181 taaaaatact gaccaaattg aataccatca gttagaaggt tttgtcagac aaattattgg
     3241 atggagagaa tacatgcgtg gtatttattg gatgaaaatg cctgagtacg caacccttaa
     3301 ttattttgaa cattctgaaa aactgccgca atggttttgg acgggaaaaa caaaaatgaa
     3361 ttgcctgaaa aatgctattc atcaatcact tgattatgct tatgcacatc acatacaacg
     3421 actaatgatt accggcaatt ttgccttgct tgcaggtgtg catcctgatg aagttgatgc
     3481 ctggtacctg ggcatttata ttgatgcaat agaatgggtt gaaattacta acaccagagg
     3541 tatgagccag tttgccgatg gcggaattgt aggcaccaaa ccgtatgtga gttctgcaac
     3601 ctacatcaat aaaatgagtt cgtattgcac aggatgttat tatgacaagg caaaaaaaac
     3661 aggagataaa gcctgtccgt ttaatagctt gtactggaat ttttatgata aacacgaagc
     3721 caaacttgcc aggaacccac gcattggaat ggtttataat gtttggaaaa aaatggaacc
     3781 taatgcaaaa gcagagttac tggaacaggc tgaatattac ttaaaccata tcaacgaatt
     3841 gtaaatgatt acgataatat aaaatattta gaaaccgatg gctgaacaaa atgtaacttg
     3901 cgagtacagc ggtttgcgct ctttggcaag tcttttagca gagcaagaga agcaagaagc
     3961 aataaaaaag caaactgaaa aattctattt tagtgaagaa gaccgagaca gaattattca
     4021 aatggcatgg gaagaccgca caccatttga agccattgaa tttcagtttg gtttgaaaga
     4081 aaaggatgtc atagaattta tgagtaaaaa ttctttgcct tccagttttc gcatgtggcg
     4141 taaacgaatg aaagcgcgca aaacaaaaca tgctgccctg cgtgatgacg gaaccaatcg
     4201 cttcaaatgc agccggcaaa ggagcaccgg aaataaaatt tcaaaccgat aacttctgtg
     4261 ttttctacaa ttaaatattt agtcatttca gttcctttaa atcattccgc aaccataacc
     4321 atatacgatt cattgttttt gtaaagagtt tatttttaga atatataact cgactgaaaa
     4381 gtattcttga aaccaaatcg ctctgaaata ttattttttc aacaggaatg ttatttattg
     4441 agatactatt ttaatccatc acaaccactc ctacattata aaagataacc ataagcacgt
     4501 tttgtatgaa aatttttatt ttatttgaca aacagtaaaa tcaatagtta tgaatttgaa
     4561 taagaattta aatcatcagg gaaaaaattt caaggtaaaa cactcagctg ccactataat
     4621 actattgtgt attttcactt ctgctttcct gctcgaatca gtaaattgtt cttcggataa
     4681 tagagcaagt aagagtgaac gcgattctac attggccgaa attgccgaaa tgtttaccga
     4741 agaaaaatta tggacagcgc ccgatatagc aaccatacca aataatgacg agggtaaact
     4801 aataaagtat ggactaaatt tagttgttca tacatcgaag tattttggcc caaagggttc
     4861 tgtttcgtca aatggtaatg gcctcaactg tcagaactgt catatggaag ccggcacacg
     4921 tccatatgga aacaatcttg gtgcggcttc aactatatac ccaaaatatt tgcctcgttc
     4981 gggcacggtg gttagccttg ccgaaaaaat aaatgaatgc ttttaccgaa gcttaaatgg
     5041 cgatattatt gatactacga gtaaagaaat gcgagctttg gttgcatata ctacatggct
     5101 tggcaaagac tttcataaag atccgaaact taaaggttcc aggggaatag acgcgccaca
     5161 ttttattgac cgtgctgccg accccgccaa gggcgagaaa acgtatgatg aactatgtgc
     5221 acgctgccat ggcaaagatg gacaaggtca atttactgaa gatgttttga aggacatcac
     5281 aaagcaacaa ggaggcattg ctactgccga tgatttatat tattatcctc cactgtgggg
     5341 cgataaaagt tacaacgcta ttgctacctt atatcgtgtt ggtaaactgg ccggatttat
     5401 aaaacacaat atgccttatc ctctaactta caaaaattcg gtgttgacag atgaacaggc
     5461 atgggatata gctgcgtata tcaatagccg cgaacgtcca gagaaagatc atagcaaaga
     5521 ttatagtatc gacatttcga aaaagcctta cgactttcct tttgcccctt atgctgatag
     5581 ttattcgcag cagcaacata agtttggtcc ctttaccgat atgccttctg caatgaaagc
     5641 ccattaaccg gtatgagaaa ttacctccaa atattttttg catgcttact tgtttttggt
     5701 tgttctcaat cgggcacaaa ttccgataac gaaaaaggtg cggaaaaaga aacagcaaat
     5761 gcacaagcaa aaaaggaaat gattgtgctt accgacaggc ctccaaatat tgaaacacca
     5821 ttaagttatt ttaaagaaga ctttacaccc aatgatgttt tttttgttcg ctggcattta
     5881 tcgggtgtgc cggcatcggt cgataccaat aaatttcggc taacaatttc gggccatgta
     5941 aaaaaagaat tgtcgctttc gctagaggat ttaaaaacaa aattcaaagc tacttctatc
     6001 gttgcacttg cagaatgtgc cggaaacgcg cgcagctttt tcgacccgcg tgtacccggt
     6061 ggacaatgga aaaacggtgc catgggcaat gcccgttgga caggcgtaaa ggtgaaagat
     6121 attcttgatg ctgccggtgt aaaacccgga gcatttgatg tggcttttaa tggtatggat
     6181 caacccccgc tagaatctgt gcccgacttt ataaaatcat tgcgcattga tcatgctatg
     6241 gatggcgagg taatgatagc atacgaaatg aatggtgcac cacttccgtt gctcaatggt
     6301 tatccgctaa agcttgtagt gccgggttgg tatgccactt attggattgg aatgcttgac
     6361 gaaataaaaa tttaccccga tacatttaaa ggttactgga tgcagaaagc atatctggtt
     6421 cccaaaggtg tgcgcaatgg aaatgagcaa cccgactcgt tggcaaaagt tacagagcca
     6481 atcagtaagc tcgatgtgcg atcaatattt gtgtcgcccg agcccgatga agtattgagt
     6541 gtaaataaaa aatgtgtagt agaaggtttg gcctttgatg ccggtgatag cattgtgaaa
     6601 gtggaaataa ctaccgatgg taaaacatgg caaacggcaa atctcgatcc tgtaattgga
     6661 aaatattcgt ggcgcagatg gaagtttgac tggacgccag caactgcagg aacgtataca
     6721 cttagcgtta aagcaaccaa tgccattggc gaaactcaac ccacacatca atggaaccgc
     6781 agtggctata tgcgaaacga aattgaaaca cttaagttaa acgtaaagta aaaagcataa
     6841 caatgatgtg ttgtaaaaaa atatttttcc ttttgtgttt cgttctattt attgcatcgt
     6901 gcaccgaaga aaaaagtgac aaacaaaaac tgacggacaa ggaaaaaaca agggccgata
     6961 ttgaatcgat gtttacaaat gcagctggcg aagaagtgta caatgcaaat tgcataacct
     7021 gccattcgct aaaatatgtt gatatgcaac caccattccc acgtaagacc tgggaaaaaa
     7081 cggtagacaa aatgataaaa aatttcgggg ctcccattcc tgattctact gccaaggtta
     7141 ttgttgaata tttaatggct gtaaaaggga tgaattaaaa gtttgcgcta attgcatcat
     7201 ttgggttcaa ataaattatt acctcttaca aaattatgta gccactttca ttgcagtttt
     7261 gttaccacaa tctatgagac ctttgttata aaactatttt gtaatatttg ttacagcagc
     7321 aaaatacttc agcatgacca attcgtaatt ccacaaaaaa aggtggccat tgatagacca
     7381 ccttttcttt ttataaaaaa taattttacg attacttgag gtcgaaacga tcgaggttca
     7441 ttattttatt ccatgctgca acaaaatcgt ttacaaattt ttcattcgca tcggcacacc
     7501 cataaacttc ggcaatggcg cgcaattcgg agttcgaacc aaaaatgaga tcggcacggg
     7561 tagcagtcca tttcaattcg cctgtggcac ggttacgtcc ttcaaacata tcctgtgcat
     7621 cggtggttgg tttccatgta gtaccaaaat caagcaggtt aacaaagaaa tcgtttgtca
     7681 gcgcttctgt attgtttgta aaaacaccat gcttcgattg attataattt gtattcaaca
     7741 cgcgcatgcc acctacaagt gctgtcattt cgggcgcggt tagcgttaat aattgcgctt
     7801 tatccaccag catttcttcc atcttcgatg tatgtttcga tttgaaataa ttgcggaaac
     7861 catcggcacc gggctctagc accgcaaacg attctacatc cgtttgctct tgtgttgtat
     7921 cggcacggcc cggtgtaaat ggcacggtag ttttaatacc tgcattgtgt gcggctttct
     7981 ctaccccggc acaaccggca agtacaatca agtcggcaag cgatatcatt tttccacccg
     8041 actgagcgct gttgaattct ttttgaattc cttcgagtac gccaattact tttgataatt
     8101 gtgcaggatt atttacctcc cagtcttttt gtggcgacaa acgtatacgg gcaccgttag
     8161 caccaccacg tttatcggaa ccacgaaatg tagcggccga tgcccatgct gttgaaacca
     8221 gttgcgacac tgtgagtccc gatgcaagta tttttgtttt tagtgcagcc gtatcatttc
     8281 catcaattaa tttatgattc actgccggaa taggatcctg ccaaatcaat tcttcctttg
     8341 gtacttccgg gccaaggtaa cgcgcaatgg gtcccatatc gcggtgtgta agtttgtacc
     8401 atgcccgggc aaatgcatca gcaaattgat cgggattttc gaaaaagcgt tttgaaattg
     8461 gagcatatat gggatccaaa cgcaacgaaa gatcggttgt gagcatcata ggtgcgtgac
     8521 gcatgttggt gtcatgtgca tccggtactg tgcctgcacc ggcatcgccc ttaggcttcc
     8581 attgatgcgc acctgcaggg cttttggtaa gttcccattc gtaaccaaat aaattttcga
     8641 aatagttgtt gctccatttt gtgggcgatg ttgtccatgc accttcgagg ccgctggtaa
     8701 ttgtatttac accactgcct gtaccaagcg tatttttcca tccaaggcct tgctcttcca
     8761 tagctgctgc ggcaggttct acaccaacgt acttacccgg gtcggcagca ccatgcgact
     8821 taccaaaggt gtgacctccg gcaataagcg caacagtttc ttcgtcattc attgccatgc
     8881 ggccaaaagt ttcgcgaatg tcgcgcgctg ctgcaattgg atcggggtta ccattggggc
     8941 cttgtggatt cacatatatc aaacccatct gcacggcacc aagtggattt tccaattcac
     9001 ggtcaccggt atagcgcttg tcgccaagcc attcaccttc gggtccccaa taaatatctt
     9061 ctgcgggttc ccacacatcg gcacggccac cggcaaagcc aaatgttttg aaacccatcg
     9121 actccaatgc acaatttccg gcaagtatca tcaaatcggc ccacgaaatc ttgttgccat
     9181 atttttgttt tataggccac aataataaac gggccttatc gaggttcgca ttatcgggcc
     9241 agctattgag cggtgcaaag cgctgtgtgc ccgaaccacc accaccacga ccatccgata
     9301 tacggtatgt accggccgag tgccatgcca ttctgataaa taagggtcca taatgtccat
     9361 aatctgccgg ccaccaatct tgcgattgcg tcatcaattc gaaaatgtct tttttcaatg
     9421 cagccaaatc gaggctctta aatgcttcgg cataatcgaa attttcgccc atcggattgc
     9481 taagcgttga atgctgacga agaatattca gcttcaacag attgggccac cagtcgaggt
     9541 ttttgggacc tgcacctgcg ctttgtttta cagcaccgcc atgaaatggg catttagcct
     9601 caccattcat cttgctttgt tctttacttt gatt
//
LOCUS       DAORQA010000056         9430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1918_length_9430_cov_15.270080, whole genome shotgun sequence.
ACCESSION   DAORQA010000056 DAORQA010000000
VERSION     DAORQA010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9430
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1918_length_9430_cov_15.270080"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="PK736_04865"
     CDS             complement(<1..1464)
                     /locus_tag="PK736_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRC32755.1"
                     /translation="MSVLLFNPRSNLYKPRIPNSILQVAASIDGIYEWIIVDGNLEVD
                     PAQKIEQLIAEKNIKYCGFTVMPGPQLKQAIPISIDLKKKFPRLKMIWGGYFATNQHK
                     TVLQSGVIDFVIDGMGDKAFPALINHLENGTDLNEVPNLIYLKNGEIIKNRKDGIYDL
                     DTLQPLPYTKLNDLYPMKKYLGKTYLGTKTIAYHSSFGCPFTCGFCAVVPIYNARWKG
                     KSAQLVYDDIMLLRNNYGGNAIEFHDNNFFVSEKRTVEFAQLIKNENMVWWGEGRIDT
                     IDKYSDESLTIIREAGCKMIFFGAETGNDEVLKMMDKGGTQTGAQIKKFAARMKTYDI
                     VPEYSFVLGAPAPTEKQVWKQIYEDIEFIKTVKQINPDVEIIIYVYSPVPSEGSEMFE
                     KVKASGFKFPATLEEWISPQWENFDLRKNPLTPWLTADMVDYIRNFETVLNGYYPTVS
                     DYKLKNWQRSALKLTSNIRYNKKLYSKPYEIKALQKLF"
     gene            complement(1698..2396)
                     /locus_tag="PK736_04870"
     CDS             complement(1698..2396)
                     /locus_tag="PK736_04870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRC32756.1"
                     /translation="MNKLSITMLALVVLFTTAAKSQTMEFKTKEDSMSYALGVSIAGN
                     LKQSNLDKGLNVDLLAAGMKAFLTGGQCKIDAAASNKMISEHMQAEQAKQAAEKAAKA
                     GENKAKGEAFLATNKAKAGVTTTASGLQYEVIKMGTGPKPTATDKVTTHYHGTLIDGT
                     VFDSSVQRGQPAQFGVNQVIPGWTEALQLMPTGSKFKLFLPANIAYGDQGAGGMIGPG
                     EALVFEVELISIDK"
     gene            complement(2567..4024)
                     /locus_tag="PK736_04875"
     CDS             complement(2567..4024)
                     /locus_tag="PK736_04875"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32757.1"
                     /translation="MKKIIVFVLCLFCLNNMMYSQCTPDNGITDPGIYPDTIENLPVA
                     FQNAFYSTTMQLKVFTDTLVPGLGLVNVTNIQILSVLGMPPGINYQCYQNNCTFPGGS
                     NRCVQISGTATTAGVYPIVVNVKLNGIYGGFIPVSQNSTVTGYTIVVLAAPVANFSAT
                     PTIICPGGAVTFADASTNMASSWNWTFTGGTPATSTEQNPVVTYSAAGQYAVTHSATN
                     PAGTNSITKTNYITVNAAPPGNIQMISNDSVCRGDTLKLLANSSPGATYQWYKSLTII
                     PGASSPYFSSITSGYFRCIVTATNGCTKAIGPKITKIVNLPIAIITAAGPTTFCQGQS
                     VVLNANTDIGLTYQWKKFSTDIANATLPSYTATQAGVYKVITRNAFGCGRTSNTIQVT
                     INCREGIFAAGNNLQLYPNPAQDFTTLEFFATADNATISIYEITGKLVDTFNASTLPN
                     TIASLNIPTAHLPGGVYMIQVDDGNNKRTVRLMVR"
     gene            complement(4347..4832)
                     /gene="cdd"
                     /locus_tag="PK736_04880"
     CDS             complement(4347..4832)
                     /gene="cdd"
                     /locus_tag="PK736_04880"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRC32758.1"
                     /translation="MQKFELKTEIEIYNSVDELNASDALLMQKAIEATRTAYAPYSNF
                     NVGAAVLLENGEIVSGSNQENAAYPSGLCAERVAVFYSGAHYPNVKIKAIAVTAFIGT
                     QPTPTPVSPCGDCRQVMAECEHRYQSEIRFIMVGDGAKIFVFNSVKLLLPFMFNADSM
                     K"
     gene            complement(4903..6837)
                     /gene="dnaG"
                     /locus_tag="PK736_04885"
     CDS             complement(4903..6837)
                     /gene="dnaG"
                     /locus_tag="PK736_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRC32759.1"
                     /translation="MIPKATVESIVETARIDEVVGDFVNLRKRGSTLVGLCPFHNERS
                     PSFAVSPSKGIYKCFGCGKAGDSVNFVMEHEHYTYPEALRYLANKYNIEIVEEIKTDE
                     QKIADLEKESLYAINDFAAKFYHQNLETTEGQAIGKAYFYERGFTDATIEKFQLGYSP
                     DAWSTFTDEATKNAYNISNLEKAGLTIIKEDKKYDRFRGRVMFPIYNLTGRTIAFGAR
                     ILKTDPHQPKYVNSPESDIYKKNKSLYGINFAKKSITVNDTCYLCEGYTDVISLHQAG
                     IENSVSSSGTSLTVDQIKLIARYTQNITVLYDGDAAGIKASMRGIDMILQEGLNVRIV
                     LFPDGDDPDSYSKKVSTEELKLFLTDSARDFILFKTELLLSEAGNDPLRKAALIKDIV
                     ESISKIPDAIIRATYTKQCAIAMEIDEQVLLIELNKLRSKSAQQTHRDNETAIEETQQ
                     IPIAEEIPQTEDNAFYQEQEVMRLILNYGDHNIVIGDDNIDIQPYKVVDFIVSELMGD
                     EISFDDELLAQMLNLCHEIYASKKTFHVDLFTKQQDDMALREKAIGLITTQYTLSEGW
                     EQRGIFTRTEDKHLRHAVLSALYTLKMKKVMQMLKETGLELKTAFDDGNDYDDIMLKY
                     QQLESIKMELSKYLSIVIVK"
     gene            complement(6987..7955)
                     /gene="rhuM"
                     /locus_tag="PK736_04890"
                     /pseudo
     CDS             complement(6987..7955)
                     /gene="rhuM"
                     /locus_tag="PK736_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655380.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(7987..9096)
                     /locus_tag="PK736_04895"
     CDS             complement(7987..9096)
                     /locus_tag="PK736_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRC32760.1"
                     /translation="MVKRILKIVLVVALLGIFVWTLYFLYKKSEQPPVVFETSTPFDT
                     TIIKKTVAAGSVIPRKEVSMKSQVSGIVEKVFVVAGQEIKSGDVIAKVKIIPNMINLA
                     SAENRVNQARINFDNAKAEYDRSETLFNQQVVSKAEFQQNELRLKTAKEEMNAADNNL
                     QLIKNGVTKSAGSATNTLIRSTINGMVLDVPIKEGSSVIESNTFNEGTSIASVANMGE
                     MIFEGKVDESEVGKIKSGMELLLTIGAIDAERFKATLEYIAPKGVTENGAIQFLIRAA
                     LTKAQGGSFLRAGYSANADIILDRKDNVFAVSESVLQFEKDKTFVEIEVAPNKFEKRF
                     IKTGLSDGVNIEVLEGITKKDKIKIPDLKTPVEKN"
     gene            complement(9159..>9430)
                     /locus_tag="PK736_04900"
     CDS             complement(9159..>9430)
                     /locus_tag="PK736_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRC32761.1"
                     /translation="PVSVMFQIILESVFLTAVAGYFGLVIGVGLLELINMALGADVPM
                     FSNPTVDINVAIVSLSVLIGCGALAGLIPAQRAVSVLPVEALRSE"
BASE COUNT         2683 a         1824 c         1656 g         3267 t
ORIGIN      
        1 aaataatttt tgtaaggctt taatttcgta tggcttgcta tacaattttt tgttgtagcg
       61 gatattgctg gtaagtttta atgccgagcg ctgccagttt tttaatttgt agtccgacac
      121 ggtggggtaa tatccgttca gcaccgtttc gaagtttcgg atatagtcaa ccatatcggc
      181 tgtaagccat ggtgtaagag ggtttttacg caaatcaaaa ttttcccatt ggggcgatat
      241 ccattcttct aatgttgcag gaaacttgaa acccgatgct tttacttttt cgaacatttc
      301 acttccttca cttggcaccg ggctgtacac ataaattatt atttctacat cggggtttat
      361 ttgctttact gttttaataa attctatgtc ttcataaatt tgtttccata cttgtttttc
      421 ggtgggtgcc ggtgcgccaa gtacaaatga atattcaggt actatatcgt aggttttcat
      481 tcgtgcggca aactttttta tttgtgctcc ggtttgtgta cctcctttgt ccatcatttt
      541 cagcacttcg tcattgccgg tttcagcacc aaaaaaaatc atcttgcacc ccgcttcacg
      601 aattattgta agcgattcgt cactgtattt atcaatggta tcgatacgtc cttcacccca
      661 ccaaaccatg ttttcgtttt ttataagctg tgcaaattct acagtacgct tttccgatac
      721 aaaaaaatta ttgtcgtgaa actctatcgc attgccacca taattgttgc ggagcagcat
      781 aatgtcatcg taaaccaatt gtgccgattt gcctttccag cgagcattat aaataggaac
      841 cacggcacaa aaaccgcaag tgaacggaca gccgaagcta ctatggtacg caattgtctt
      901 agtgccaaga tatgttttac ccaaatattt tttcatcggg tataagtcat tgagcttcgt
      961 atatggtaat ggttgcaggg tatctaagtc ataaattcca tccttgcggt tttttataat
     1021 ctctccattt tttaaataga ttaaattagg cacctcattt aaatctgttc cgttttcgag
     1081 gtgatttatt agtgcaggaa atgctttatc gcccatacca tcaattacaa aatcgataac
     1141 accactttgc aaaacagttt tgtgttgatt ggtagcaaag taaccacccc atatcatttt
     1201 caagcgggga aatttctttt tcaaatcaat gcttatgggt atagcttgct ttaattgtgg
     1261 ccccggcatt actgtaaagc cgcaatattt aatgtttttt tcggcaatta attgctcgat
     1321 tttttgtgcg ggatcgactt ccaaattgcc atcaactatt atccattcat atatgccatc
     1381 aatgcttgcc gcaacttgca atatcgaatt aggaattcgg ggtttatata aattgctacg
     1441 aggattaaac agcagaaccg acatatttat tttgatattg tttttatgcg cacaagttta
     1501 gctcaaatat cgattacaag attaataaaa atagatagag ttgcgttatt aatttagtga
     1561 gttgtcgttt ttatttttgg cgcccttttt ttattgaatt aatatgacac tcaagtactg
     1621 cattgtttat aaaataatat agaaagcaaa aaaaatcccc gcttaaagcg aggatttcat
     1681 tgagaggatc tgttttttta tttgtcgatg gaaattaatt ctacctcgaa tacaagtgct
     1741 tcacctgggc ctatcattcc accagcacct tggtcaccgt aagcaatatt tgcaggtaaa
     1801 aacaatttga attttgaccc tgtaggcatt agttgtaaag cttctgtcca tcccggaata
     1861 acctgattaa cgccaaactg tgcaggttga ccacgttgca ccgaactgtc gaacacagta
     1921 ccatctatca atgtgccatg ataatgtgtt gtcaccttat cggttgcggt aggttttggc
     1981 cctgttccca ttttaatgac ttcgtattgc aaaccgctgg cagttgtggt tacaccggct
     2041 ttggctttat ttgttgctaa aaatgcttca cccttagctt tgttttcacc agcttttgct
     2101 gctttttcgg ctgcttgctt tgcttgttca gcttgcatgt gttcgcttat cattttgttg
     2161 gaagcagctg catcaatttt gcactgtcct ccggtaagaa atgctttcat gccggcagca
     2221 agcaaatcta catttaaacc tttgtcgagg ttgctttgtt ttagattacc agcaattgat
     2281 acaccaagtg catagctcat tgagtcttct tttgttttaa attccatagt ttgcgatttt
     2341 gcggcagttg taaaaagtac aactaaagcc aacattgtta ttgataattt gttcattttg
     2401 tttttttact ttttttttaa atgaggctgc gaaaatagta ttttttccat aaaagtaaat
     2461 atgcaaattg gatgaaagtt ttcctgcttg cttttatgac cgaatgttat tttcttacta
     2521 acaaaataat aattattgac cgttaaatca accgtacaag caaacattac ctcaccatca
     2581 aacgcacagt acgtttatta ttaccatcgt ccacttgtat catgtaaacc cctccaggca
     2641 agtgagctgt tggtatattt aaagaagcta tagtgtttgg caatgttgaa gcattaaaag
     2701 tatctaccag tttaccggta atttcatata tacttattgt ggcgttatca gcagtagcaa
     2761 aaaattctaa cgtggtaaaa tcctgagccg gattgggata caattgcaaa ttgtttcctg
     2821 cggcaaaaat tccttcgcga caatttatgg taacttgtat ggtgtttgat gttcggccac
     2881 aaccaaatgc attgcgggtt ataaccttgt aaacccctgc ctgagttgcg gtataagatg
     2941 gcaatgtcgc attagctata tcggtactga attttttcca ttgatatgtt aagccaatat
     3001 ctgtattagc gtttagcacc acactttggc cctgacaaaa tgttgtaggc ccggcagcag
     3061 taatgattgc aattggcagg tttactattt tcgtaatttt tggccctatg gctttggtac
     3121 aaccattggt ggcggttacg atacatctga aataaccgct tgtaatagag ctaaaatagg
     3181 gtgacgatgc accgggaata atagtcaggc ttttgtacca ttgataagtt gcaccagggc
     3241 ttgagttagc caacaatttc aaagtgtcgc cacggcatac actatcgttc gatatcattt
     3301 ggatattacc cggtggcgct gcattcaccg ttatataatt tgttttggtt attgaatttg
     3361 ttccggcagg attggtggca ctatgtgtaa cagcatattg gccggcagcg ctatacgtta
     3421 ccacaggatt ttgttcggta gaagttgcag gtgtgcctcc ggtgaaagtc cagttccagc
     3481 ttgaagccat attggtactt gcatctgcaa aagtaaccgc accaccgggg caaataattg
     3541 tgggtgtagc gctaaagttg gcaacaggtg cggccaacac aactatagta tagcccgtaa
     3601 ctgtagagtt ttgagataca ggaatgaagc caccataaat gccattcagc ttaacattta
     3661 ctactattgg ataaacccct gcggttgttg ctgttcccga tatttgaaca catctgttac
     3721 tgccaccagg aaaagtgcaa ttgttttgat aacattggta gtttataccc ggaggcattc
     3781 ctaaaacact taatatctgt atgtttgtta cgtttactaa tcctaagcca ggcaccaaag
     3841 tatcggtaaa caccttgagt tgcatggttg tagagtaaaa ggcattttga aatgctaccg
     3901 gcaaattttc gatagtatcg gggtaaatac ccggatcggt gatgccattg tcgggcgtac
     3961 actgactgta catcatgtta ttcaaacaga ataagcataa aacgaaaacg ataatttttt
     4021 tcataagcat ttgatttttt ttagaagtta aaatttgatt ttggagcaag ccaaagtata
     4081 tataaaattg gaattacaaa tatatttttc tgtaaatatt tttttgacaa caaataatgt
     4141 gacagttcat tcacaaaaca gaaaaatata attgttgcca tcaaacctta aaaaagttga
     4201 gaattcattt tttaaagtga ttgaaaaaca gaaaaaaact ccatgctttt tgttgcaaaa
     4261 aatatttttg catttttcgt gtattctgtg gcaaaaaata tccggcataa atttacatcc
     4321 ttaagaagag caggatggca aaaatattat ttcatgctat cggcattaaa cataaatgga
     4381 agtaaaagtt ttacactatt gaacacgaat atttttgctc catcgcccac cattataaaa
     4441 cgtatttccg attgatagcg atgttcgcac tctgccatta cctgtcggca atcgccacat
     4501 ggactaactg gtgtaggtgt aggttgtgtg cctatgaaag cagtaaccgc tatggctttt
     4561 atttttacgt ttggataatg tgcaccacta taaaaaacgg caacgcgttc agcgcacaaa
     4621 cccgaaggat aagcagcatt ttcctggttg cttcctgata cgatttcgcc attttctaac
     4681 aacaccgcag cacctacatt aaagtttgaa tatggtgcat aagcagtgcg cgtagcttca
     4741 attgcttttt gcattaataa tgcatcgctc gcattaagtt catcaacgga gttgtatatt
     4801 tctatttcgg ttttaagttc gaatttttgc atcacaaaaa aattattggt aaatatagca
     4861 agttattttt atagcctgcg aatttttata aattgccatg cattacttta caattactat
     4921 acttaagtat ttactcagct ccatttttat actttccagt tgttggtatt taagcattat
     4981 atcatcgtaa tcgtttccat cgtcaaaagc agttttaagt tcgaggccgg tttcttttag
     5041 catttgcatt acctttttca ttttcaaagt atacagcgcg ctcaatacag catggcgcaa
     5101 atgtttgtct tcggtgcggg taaaaattcc gcgttgctcc cagccttcgc tcagcgtata
     5161 ttgcgtagta ataagtccaa tggctttttc gcgcaaggcc atatcgtctt gctgcttagt
     5221 aaacaaatca acatgaaatg tttttttgct ggcataaatt tcgtgacaca aattcaacat
     5281 ctgcgcgagc aattcatcat caaaacttat ttcatcaccc attaattcgc tcacaataaa
     5341 atcaaccacc ttgtatggtt gtatatctat attgtcgtca cctatcacta tattatgatc
     5401 gccatagtta agtataagcc gcataacttc ctgttcctga taaaaggcat tatcttcagt
     5461 ttgtggtatt tcttctgcaa tgggaatttg ctgagtttct tcaatggcag tttcattatc
     5521 acgatgggtt tgttgtgcac tttttgagcg taatttattc aactctatca gcaaaacctg
     5581 ctcatcaatt tccattgcaa tggcgcattg ctttgtatac gttgcccgaa tgattgcatc
     5641 aggaattttc gaaatacttt ctacaatatc tttaataagt gcagctttgc gtaaaggatc
     5701 gttgccggct tcgctgagta aaagttcggt tttgaataaa ataaaatcgc gggcactatc
     5761 agtcaaaaat aattttaatt cctcggtcga tacttttttc gaatagctgt caggatcatc
     5821 gccatcagga aaaagtacaa tgcgaacatt aagaccctct tgcaaaatca tatcgatacc
     5881 acgcatggat gctttgatac ctgcagcatc gccatcgtac aaaacggtaa tgttttgggt
     5941 gtagcgtgca attaatttta tctgatcaac agtgagcgaa gtgccgctcg atgatacaga
     6001 attttcgata ccggcctgat gcagtgaaat tacatcggta taaccttcgc ataaataaca
     6061 ggtatcattt actgtaatac tttttttggc gaagttgatt ccgtaaaggg atttattttt
     6121 tttgtaaata tcactctctg gcgagttgac atattttggt tgatgtggat ccgttttaag
     6181 aatgcgtgcc ccaaatgcaa tggtgcgtcc tgtaagattg taaataggaa acataacgcg
     6241 gccacgaaaa cggtcatact ttttatcttc tttaattatg gtgagtccgg ctttttcaag
     6301 attacttatg ttgtaagcat tctttgtagc ttcatcggta aaggtgctcc acgcatcggg
     6361 agaataacca agttgaaatt tttcgattgt tgcatctgta aatccacgct cataaaaata
     6421 tgctttacct attgcctgcc cctcggttgt ttctaaattc tgatgataaa attttgctgc
     6481 aaaatcgttg atagcatata aactttcttt ttcgagatcg gcaatttttt gctcgtctgt
     6541 ttttatttct tcaacaattt caatattata tttatttgcc aaatagcgca atgcttcggg
     6601 gtaggtgtaa tgctcatgtt ccattacaaa atttactgaa tcgccagctt tgccacaacc
     6661 aaaacatttg taaattccct tcgaaggtga tacagcaaac gatgggctgc gttcgttatg
     6721 aaatggacac aaacccacaa gtgtgctacc acgctttcgc aagttaacaa agtcgccaac
     6781 tacttcatct atacgtgcag tttctactat gctttcaact gtggcctttg gtatcaatta
     6841 tttttttgct tgaaattttt tttacaaaaa tataaaaatg gatgtgtgga gaaaatttat
     6901 attttcaatt gagtttaaga aaaattaaaa acacaatttt aggtttcgca ttttacagat
     6961 tgataacatg caataattcg attgctttaa tttgcatcgt taagcaaatt tgcaatcaat
     7021 tttatcatta aatctttttc gttaggattg ctttgtgcaa ccaataatgc taaagcggtt
     7081 aatccattat catttatttt tagttctcca ctttttttaa aatgatgttt gtttttttct
     7141 aagaaccaaa caaataaaaa tgcgccaatc cgtttatttc catccgaaaa ggaatggttt
     7201 ttaattacaa agtacagcaa atttgccgct tattcttcga tgcttggata taaatattgt
     7261 ttgtcaaatg tttgtactat gctcattaaa gagcttttaa agccttcatc tttttctttt
     7321 ccaaataact ctgtggcttc gttcttaatt attaattggc gtttcaattc cgaaatcgca
     7381 tattttgctt cttcatattg gatttcataa gttatatttt cgcttagatt ttgattcgaa
     7441 atagcgtttc tgtcatattg atttagcaaa acaaaactct tagtatattt acttaatatt
     7501 tccaacaatc cttttgcttc ctgcaactga attgtatcaa attgcgttgc attttgaacg
     7561 agctgaacca tctgtccaag ttctgctagt cttttttgat ttatggtata accttgaagt
     7621 aaatgttctt tgagcctttg ggttgcccat tgacgaaatt gagtacctcg cttagatttt
     7681 acacggtagc ctactgaaat tattacatca agattgtaat gttccatttt ataggttttt
     7741 ccatcactcg cagttgtcgc aaattttgcg acaactgaag attcaatcaa ctcaccttct
     7801 aaaaatacat tatttatatg ctttccaata gttttaatat ctctatcaaa gagtatggaa
     7861 agttgttgtc tactaagcca aaaggtttcg ttttcaaatt gaacataaac ttgaggttga
     7921 ttatcttttg attgataaat tattatttca ctcattttgg ataataaaaa attaattata
     7981 acacttttaa ttcttctcca ccggtgtttt caaatcagga atttttattt tatctttttt
     8041 cgttatgcct tcgagtactt caatatttac tccgtccgat aaaccggttt ttataaatcg
     8101 tttttcaaat ttatttggcg ctacttcaat ctctacaaaa gttttatctt tttcgaattg
     8161 taaaaccgat tcggaaaccg caaagacatt atcttttcta tccaaaatta tatcggcatt
     8221 ggcgctataa cctgcgcgta aaaaagaacc tccttgtgct ttagttaagg cagcacgaat
     8281 aagaaattgt atagcgccat tttccgtaac acccttgggc gcaatatatt cgagtgttgc
     8341 cttgaagcgt tcggcatcta tagcgccaat ggtcaacaac aattccattc ccgattttat
     8401 tttgccaact tcactttcat caaccttgcc ttcaaaaatc atttcgccca tattggctac
     8461 cgatgctatg gaagtacctt cgttaaaagt attgctttct attacactgc ttccttcttt
     8521 aatcggcaca tcgagcacca ttccatttat agtactccgt atgagtgtat tggtagcact
     8581 gccagccgat ttggtcacac cgttttttat aagttgcaaa ttattatctg ctgcattcat
     8641 ttcttctttt gcagttttca atcgcaattc attttgctga aattcggctt tcgaaactac
     8701 ttgttgattg aataatgttt cgctgcgatc gtactctgct tttgcattat cgaaattaat
     8761 gcgcgcctga ttcactctgt tttcggcact tgcaaggttt atcatattgg gaatgatttt
     8821 taccttagca attacatcac ccgattttat ttcctgcccg gcaaccacaa atactttttc
     8881 tacaataccc gatacctgag atttcatact cacttctttg cgtggtataa cgcttccggc
     8941 tgctactgtt ttttttatta tagttgtatc gaatggagtt gatgtttcaa acaccaccgg
     9001 aggttgttcc gattttttgt aaaggaaata aagtgtccac acaaaaattc ctaacagtgc
     9061 aaccactaaa acgattttta aaattctttt taccataaaa tatttttcaa tacaattttt
     9121 tctaaaacaa tgatttgaaa tctgtaactt tttaaaattt attcggatcg caaagcttca
     9181 accggcaata cagaaacagc acgctgtgca ggtatcaatc ctgcaagtgc cccacaacct
     9241 atcaacacag aaagtgaaac aatggcaaca tttatatcca ctgttggatt gctaaacata
     9301 ggaacatcgg cacccaatgc catattaatt aattcgagca agcccacgcc tattaccaag
     9361 ccaaagtatc cggctactgc agtaagaaaa accgactcca gtattatttg aaacataaca
     9421 ctcacaggag
//
LOCUS       DAORQA010000057         9323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1941_length_9323_cov_11.887246, whole genome shotgun sequence.
ACCESSION   DAORQA010000057 DAORQA010000000
VERSION     DAORQA010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1941_length_9323_cov_11.887246"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="PK736_04905"
     CDS             <1..1245
                     /locus_tag="PK736_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRC32762.1"
                     /translation="DMFSEVRKDIISTHQQTKNSYIDSLNKLLKNNAIVKKDSDVVTS
                     HGLEPFLLALNNLPKAKNKIRIAWFGDSMIEGDLVTNDFRKLMQNTFGGNGVGYVGVT
                     SPTAQFRGSINHTFSGWKTYSFHDKPPKGLQLSMSGYTNINNGNAKVKYTASKEYNAF
                     GDVRLLFSSSDYGSINVQADTFEREVFIDSINGTGTVAIWDSTPCKKLIVDFNKTQAQ
                     YYGMYFDNGTGVYVDNFSFRGNSGIPLSEIPTTMLQKMSKDENYQLVVLNYGLNIVAH
                     NQKNYDWYERAFKKTLAHIKQGFPNATIVLMSVSDKSYRNNGVYETEPDIPKFVELQK
                     KMAQEAGISFWNLYEAMGGKNSMKRWVEEKPRLANYDYTHPNFDGAKKIATLFYDYLL
                     AEYSAFLKQKKLQTDTLIQTNL"
     gene            1246..2493
                     /locus_tag="PK736_04910"
     CDS             1246..2493
                     /locus_tag="PK736_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRC32763.1"
                     /translation="MTIKSLILFLLCVCANLICIAQFAGNNEEELLLQQMHKMKFINA
                     NAFCILNDSTLGSFYKKLSQLEETKQGKVNVVHIGDSHIQAGYFTGILRRMLQRKYGN
                     GGRGVMFPYRIAKSNGPFDYVSATNTTWQHRRNLFADSAFTTGLAGFTIRADSSDATF
                     CLTLRNNEGFNYDYSHLDLFTHENQWLQISVTDSTANIAAHYDGINSTKNCHRYNFDT
                     LINSACIRLHTSDRAFPISVSGINTYNDSAGVICHTIGVNGAQYKHINNQPLFFEHLT
                     QLNADIIILSFGTNEAYTVKNFKTEEFLATVDSTVQLIKKIYPTIPVLISTPAESGIK
                     YRKRKYKVHPNMAKVHAALVQYCKEKNIACFDLYEAAGGANSFFQWRKMKMMDANAIH
                     FTRKAYEMQAIMLWQSMFKKPMP"
     gene            2495..3973
                     /locus_tag="PK736_04915"
     CDS             2495..3973
                     /locus_tag="PK736_04915"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRC32764.1"
                     /translation="MTIDFYKVASNFLYDKSNPLLFNSSFYLYFLVFFLLAYRLVIKT
                     GLARIVTVTLFSLYFFYKACGFYVIFILIAAVVDFNLSNAIHRSENKTQRKLLLIFSI
                     VLNLVFLCYFKYTNLFIDTWNNFSQHQLKLIDIFLPVGISFYTFENLSYTIDVYRKRF
                     NPVTKFIDYLFFLSFFPKLMMGPIVRAGDFIPQIYKEVKLSATAITKGLFLISTGIFK
                     KVVISDYIYLNLVQYVFDDPLRHTGMECLLAVYGYALVIFCDFSGYSDMAIGIAKWLG
                     FDINVNFLAPYQSKNITEFWRRWHISLSSWLKDYLYISLGGNRLGKTRTYINLFITML
                     LGGLWHGANWTFVVWGAMHGLALAAHKKWMELFPPVEGKKNPMWLNIAFVLLTFHFVC
                     FTWIFFKADSFATAIDFLHQIFFNFYIQGWAKFSANYAPVLAVMAFGFFIHMVPLRWN
                     NIIVNLMTPWHWIFKAAVLAIMVFIIIQFKQSEPVLPVYLQF"
     gene            4514..4978
                     /locus_tag="PK736_04920"
     CDS             4514..4978
                     /locus_tag="PK736_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32765.1"
                     /translation="MIRNTQRSIGKPLKGFGAYSIARQCPISGGNAVFKARPVFYLVQ
                     PNYKFDDRLLCNRVGIPLRKPNVDKQLSKGFKIYPNPTSQSFTVEYAIDKNCESVLYI
                     TNSIGQTQLTKAIDNNKNSIVINSENYASGIYSCRIICAENILFQQKFIIIK"
     gene            4984..6486
                     /locus_tag="PK736_04925"
     CDS             4984..6486
                     /locus_tag="PK736_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32766.1"
                     /translation="MKRILIIVIIFGLQCDLLSQGINSKGLFGYQSNAGGNFGNTWMD
                     FQGTNHTFEYDSLETGVAATNTVICDSLGNLLFYTNGWEVADSTGYIMLNGANISTTT
                     YQATIFPPGFGMDVPQANMAIPYPSSNGKYYLFHSTRDGPQYKYTYKLDYSIIDMAGN
                     NGLGEVISKNNAIIVDTLNFGKIIACKHGNGRDWWVTCLQMNSNLIYKLLVTPYGING
                     PYSQSIGWVKARGYGECKFSPDGTKFAWFDADYTKPGHAEIMDFDRCSGTFSNPLHME
                     ISQSNGFGGGVCFSPNSQLLYIANVDSVYQYDLNAANIEQSKVFIAAWDSFYSPYPPF
                     GTYFYMTLNAPDGKIYISTGNSTKHLHVINNPDLLGIGCNLVQHAVALPNYYSNSLPN
                     HPNYFLGDNGLCNNLGIPPAPYEGVKKIQVFGNPTHDKFTLWFPVDKDVGVLEIYDVN
                     GECIRRERVAQWSQYKTVDISGLSEGVYFCRMSWPDGEGSVKVVRTTDGN"
     gene            6840..7349
                     /locus_tag="PK736_04930"
     CDS             6840..7349
                     /locus_tag="PK736_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRC32767.1"
                     /translation="MRKLLMALCVGVALSATMYAQDKGTQKLASAKVKTLDGKLFSTD
                     SLQNNGKPMIVSFWATWCKPCINELNTISEVYEEWKTETGVKLVAISIDDARTMNSVK
                     PFINGKNWPYEVYLDPNSDFKRAMNVGPVPHTFLVNGKGEIVSQHTSFSPGDEEKLLE
                     EIKKLVAGN"
     gene            complement(7490..7828)
                     /locus_tag="PK736_04935"
     CDS             complement(7490..7828)
                     /locus_tag="PK736_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRC32768.1"
                     /translation="MSKEPTTYLKHIQDECSYIISVSDNLTFENFIVDETLKRAVVRS
                     LEIIGEATKKIPADFKLTWSAIQWKNIAGMRDRLIHDYMGVNYAIVWDVMKNKIPDMH
                     KKITGFLSHN"
     gene            complement(7821..8117)
                     /locus_tag="PK736_04940"
     CDS             complement(7821..8117)
                     /locus_tag="PK736_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018619929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRC32769.1"
                     /translation="MTTKDDILKKLHANMHMLSKLGIRNVGLFGSYIRNEQSNDSDID
                     LLIDFEPEKENFDNFMAVYDLLENIFKNEKVEVVTKNGLSPYIGPNILNEVKYV"
     gene            complement(8216..>9323)
                     /gene="lysS"
                     /locus_tag="PK736_04945"
     CDS             complement(8216..>9323)
                     /gene="lysS"
                     /locus_tag="PK736_04945"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRC32770.1"
                     /translation="IKLLTKSLRPLPIIKEKDGEKYDEVTDSELRYRQRYVDLIVNPS
                     VRDVFRKRTQLMNVMRNYLNEKGYLEVETPILQPLYGGAAARPFKTHHNTLDMTLYLR
                     IANELYLKRLIVGGFDGVYEFSKDFRNEGMSRFHNPEFTQIELYVAYKDYNWMMNLVE
                     EVIEKIALELHKTTEVKVGENIIDFKRPWKRYTMYEAIQHYTGIDISEMDEAQMREVC
                     AKLNIHADNSMGRGKLIDEIFGETVEHQLIQPTFITDYPVEMSPLAKKHRSKPGLVER
                     FEAVCNGKEICNAFSELNDPIDQRERFEEQLELGKRGDEEAMQLDEDFLRAIEYGMPP
                     TAGLGIGIDRLAMIMTNQPSIQDVLFFPQMRKEV"
BASE COUNT         2880 a         1632 c         1805 g         3006 t
ORIGIN      
        1 gatatgttta gcgaagttcg caaggatatc atttctaccc atcaacaaac aaaaaactct
       61 tacattgata gcttaaacaa attattaaaa aataatgcta ttgtaaagaa ggacagtgat
      121 gtagttactt cacatgggct ggaacctttt ttactggcac ttaataattt gccaaaagca
      181 aaaaataaaa ttcgtatagc atggtttggc gatagcatga ttgaaggcga tttggtaacc
      241 aacgattttc gtaagctaat gcaaaatact tttggcggca atggtgtagg atatgtagga
      301 gtaacctctc ccacggcaca gtttcgcggg tctattaatc atactttttc gggatggaaa
      361 acatattctt ttcacgacaa acctcctaaa ggattgcaac tgagcatgtc gggatatacc
      421 aatattaata acggcaatgc caaagtaaaa tacacagcat cgaaagagta caatgccttt
      481 ggagatgtgc gcttattatt tagcagcagc gattacggaa gcataaatgt acaggccgat
      541 acatttgagc gcgaagtttt tattgattca ataaatggca ccggcacagt agcaatttgg
      601 gatagcacac cgtgtaaaaa attgattgta gattttaata aaacacaagc acagtattat
      661 ggcatgtatt tcgacaacgg caccggagtg tatgttgaca atttttcgtt tcgtggcaac
      721 tcaggaattc cattaagcga aataccaacc actatgctgc aaaaaatgag taaagacgaa
      781 aactatcagt tagtggtatt gaattatggt ttgaatatag tggcgcacaa tcaaaaaaat
      841 tacgactggt acgaacgggc atttaaaaaa acactggcac acataaagca gggttttccc
      901 aatgcaacta ttgtattgat gagtgtgagt gataaaagtt atcgcaacaa tggcgtgtac
      961 gagaccgaac ccgatatacc aaagtttgta gaattgcaaa aaaaaatggc gcaggaagca
     1021 ggaatatcgt tttggaattt gtatgaagcc atgggtggaa aaaacagtat gaaacgttgg
     1081 gttgaagaaa aaccacggct tgcaaactac gattacacac atccgaattt tgacggagcg
     1141 aaaaaaattg ccacactttt ttacgattat ttgctggcag aatacagtgc gtttttaaaa
     1201 caaaagaaat tgcaaaccga tacattgata caaacaaatt tataagtgac tattaaatca
     1261 ttaatacttt ttttattatg tgtttgtgcc aacctgattt gcatagcaca gtttgccggc
     1321 aacaacgaag aggaactttt gttacagcaa atgcataaga tgaaatttat aaatgccaat
     1381 gcattttgta ttttgaatga tagcaccctc ggttcgtttt ataaaaagtt aagccaactt
     1441 gaagaaacca aacagggaaa agtaaacgtg gtgcacatag gcgattcgca tattcaagcg
     1501 ggatatttta ccggcatact tcgcagaatg ttgcaacgca aatatggcaa tggcggccgt
     1561 ggagtaatgt ttccatatcg cattgcaaaa tcgaatggcc cgttcgatta tgtaagtgcc
     1621 acaaacacta catggcaaca caggcgcaat ttgtttgccg atagcgcttt tacaactggc
     1681 ctggcaggtt ttaccatccg agccgacagc agcgatgcaa ctttttgcct cacgctgcgc
     1741 aataacgaag gcttcaatta cgattacagt cacctcgatt tatttactca cgaaaatcaa
     1801 tggcttcaaa taagtgtaac cgacagcacc gcaaatattg ccgcacatta tgacggaatt
     1861 aattctacaa aaaattgtca ccgatacaat ttcgatacgc ttattaacag tgcatgtata
     1921 cgcttgcaca caagcgatag ggcttttcca atcagtgtgt cgggcataaa tacgtacaat
     1981 gacagtgcag gcgtaatttg ccataccata ggagtgaatg gcgcgcagta caaacacata
     2041 aataatcagc cgttattctt tgagcacctc acgcaattga atgccgatat tattatactt
     2101 tcgtttggca ccaatgaagc ttacactgta aaaaatttta agaccgaaga atttttagcc
     2161 acagttgaca gcacagttca attgataaaa aagatttatc caacaattcc tgtattaatt
     2221 tctacccctg ccgaatcggg aataaaatac cgtaagcgta aatacaaagt acacccaaac
     2281 atggccaagg tgcatgcagc gttggtgcaa tactgtaaag aaaaaaatat agcctgtttc
     2341 gatttatatg aagcagccgg aggggcaaat tctttttttc agtggcgtaa aatgaaaatg
     2401 atggatgcca acgccataca ctttacgcgc aaagcatacg aaatgcaagc catcatgtta
     2461 tggcagtcga tgtttaaaaa accgatgcca tagtatgacc atagattttt ataaagtagc
     2521 atccaatttt ttgtacgata aaagcaatcc gttgcttttc aatagttcat tctatttata
     2581 ctttcttgtt tttttcttat tggcttaccg gttggttatt aaaacaggac ttgcgcgtat
     2641 agttaccgtt acattatttt cactttactt cttttataaa gcatgcgggt tctatgtaat
     2701 ttttattttg atagcggccg ttgtcgattt taatttaagc aatgccattc atcgttccga
     2761 aaataaaacg caacgaaagc tattactcat attcagcatt gtgctcaacc tggttttttt
     2821 gtgttatttt aaatacacca atttatttat cgatacatgg aataattttt cgcaacatca
     2881 gttaaagctg atcgatattt ttttacctgt gggtatttcg ttttatactt tcgaaaattt
     2941 gagctacact attgatgtgt atcgcaaacg atttaatccg gttacaaaat ttatcgacta
     3001 cctgtttttc ctttcgtttt ttccaaagct gatgatgggg cccattgtgc gtgccggtga
     3061 ttttatacca cagatatata aagaagtaaa actttcggca acggctataa ccaaagggtt
     3121 atttttaatt tcgacaggca tatttaaaaa agtcgtaata tccgattaca tttatcttaa
     3181 tctggtgcag tatgtttttg atgatccact gcgccacacc ggtatggaat gtttacttgc
     3241 tgtgtatgga tatgcgttgg ttattttttg cgatttctcc ggctactccg acatggcaat
     3301 tggtattgca aagtggttgg gcttcgatat aaatgtaaac tttcttgcac cctatcaatc
     3361 gaaaaacatt acagagtttt ggaggcgatg gcatatatca ctttcatcgt ggcttaaaga
     3421 ttatttgtac atttcgctgg gaggtaaccg acttggcaaa acacgcacat atataaatct
     3481 gtttattacc atgttgcttg ggggcttgtg gcatggcgcc aattggacgt ttgtggtatg
     3541 gggtgcaatg catggtcttg ccttggcagc acataaaaaa tggatggaat tgtttccacc
     3601 ggtcgaaggc aaaaaaaatc cgatgtggtt aaatatcgct tttgtgttgc ttacttttca
     3661 tttcgtttgc tttacatgga tattttttaa agccgattcg tttgctacgg ccattgattt
     3721 tttacatcaa atatttttta atttttatat acagggttgg gcaaaattta gtgccaacta
     3781 tgcaccggtg ttagcggtaa tggcgtttgg ctttttcata cacatggttc cattacgttg
     3841 gaacaatatt attgtaaatc ttatgactcc atggcattgg atttttaaag ctgctgtact
     3901 ggccatcatg gtttttatta tcatccaatt caaacaatcg gaacctgtgt tgccggttta
     3961 tttgcagttt taggtttttt ttgagtgctt tttttattgg caaaaattta ttgttcgcct
     4021 ttgttttgaa acatactttc tcatgttgct gaataaagtt ttttctgttt tatttgattg
     4081 caaaagagtg gcatgcatca aaatattgtg tactcaaaat tatgctgtaa atttagaatg
     4141 gtctattttt ctaaactgcg gattacgcaa aatatttatt ttacaaaacc ctttacattt
     4201 cgaatgcata tgtttacttt ggcattcaca taaacaacaa ttcatttcca tgttagctat
     4261 tatttatatt ttactttctc cgattttatg tatagtgagc gaagcgaaac tattagttcc
     4321 gtttgcttgt aagtgctgtg ggtgctgatt ctatgaatca tcgaattacg agttagtgcg
     4381 catatacgaa tgaaaaggat aattaaattc gatgattaaa cgggtcttat tattttgaaa
     4441 attatgcaga gtatgctgca attgaattat attttaaaca atttaataaa tagtatttga
     4501 aacaaaaagt gaaatgataa gaaataccca gcgcagtata ggtaagcccc ttaaggggtt
     4561 tggggcgtat tccattgcaa ggcaatgccc cattagtgga ggcaacgctg tgtttaaagc
     4621 aagaccggtg ttttatttag tacaaccaaa ttacaaattt gatgataggt tgttgtgtaa
     4681 cagagttggt attccattgc gtaagccaaa cgtagataag caacttagca aagggtttaa
     4741 aatatatcct aacccaactt cgcaatcatt cacggttgaa tatgccattg ataaaaattg
     4801 cgaatctgta ttgtatataa caaatagcat tggccaaaca caattgacaa aagcaattga
     4861 taacaacaaa aattcaattg ttattaatag tgaaaattat gcaagtggca tatattcatg
     4921 cagaataatt tgtgctgaaa atatcttatt ccaacaaaaa tttatcatta taaagtaatt
     4981 ggtatgaaaa ggatattaat tattgtaata atttttggtt tgcagtgtga tttgcttagc
     5041 caaggtatta attccaaagg gctatttggc taccaaagta atgctggtgg caattttggt
     5101 aatacttgga tggattttca aggaactaac catacttttg agtacgatag ccttgaaaca
     5161 ggtgttgctg caacaaatac tgttatttgc gattctttgg gcaatttatt attttataca
     5221 aatggctggg aagtggcaga tagcacaggt tatattatgc tcaatggtgc gaatattagc
     5281 actactactt atcaagcaac aatatttcca ccaggttttg gcatggatgt accacaagca
     5341 aatatggcca taccttaccc aagtagtaat ggtaagtatt atttatttca cagtacgcgc
     5401 gatggcccac aatataaata cacttataaa cttgactaca gcataataga catggcaggt
     5461 aataacgggc ttggtgaggt aattagtaaa aataatgcaa taatagttga tacgctaaac
     5521 tttggaaaaa taatagcgtg caagcatggc aatggtagag attggtgggt aacatgttta
     5581 caaatgaata gtaatttgat ttataaatta ttggttacac cttatggcat aaatggccct
     5641 tactcacaga gcataggatg ggtaaaagca cgaggttatg gcgaatgcaa attttcgcct
     5701 gatggtacta agtttgcgtg gttcgatgcc gattatacca aacccggtca tgctgaaatt
     5761 atggattttg ataggtgtag tggcacattt agcaatccac tgcacatgga aatatcacag
     5821 tccaatggtt ttgggggtgg ggtttgtttt tctcctaatt cgcaactttt atacattgca
     5881 aatgttgatt ctgtttatca atatgatttg aatgcagcta atatagaaca aagcaaagta
     5941 tttattgccg catgggatag tttttattct ccttatcctc cgtttggcac atatttttat
     6001 atgacgctaa atgcaccaga cggtaaaata tatataagca ctggaaatag caccaaacac
     6061 ttgcatgtaa taaataatcc tgatttgctt ggcataggat gcaatttggt gcaacatgcg
     6121 gttgcattac ctaattatta ttcaaattct ttacccaacc accccaatta ttttttgggg
     6181 gataatgggt tgtgtaataa tttggggata ccccctgccc cctacgaggg ggttaagaaa
     6241 atacaagtgt ttggtaatcc tacgcatgat aaatttacat tgtggtttcc ggtggataag
     6301 gatgtgggtg tattggagat atatgatgtt aacggtgagt gtatacgtag ggagcgggtg
     6361 gctcagtgga gccagtataa gactgtggat attagtgggc ttagtgaggg tgtttatttt
     6421 tgtaggatga gttggcctga tggtgagggt tcggtaaagg tggtgagaac cacagatggg
     6481 aactgattgg agcggatggg aacagatgat ttattttgca tttgtatttt ccgttgttat
     6541 ccgtttcatc tgctaaatcg gtggttcaaa tatttgctgc tgcaagcgtc catacttgtg
     6601 gcagtttata ttgtattgct tcaatagaac aaatctttga aaaaaaatga atggagcatg
     6661 cacaacattg ctacatgagt ggcagcttgc ggtagcgtct tcgcaactca attcttaatc
     6721 cttcattttt aatttcccgg ccctctttct atttttttaa tgaacacatt tttacaaccg
     6781 aaaattaaaa tacctttggc atggttttat caaatagtaa taaaaaacaa taaatgaata
     6841 tgagaaaatt attgatggct ttgtgtgtag gtgttgcact atcagctacg atgtatgcac
     6901 aggataaagg cacccaaaaa ttagcaagtg caaaagtaaa aacgcttgat ggtaaattat
     6961 tttccaccga tagtttgcaa aataacggca agccaatgat agtaagtttt tgggctacgt
     7021 ggtgcaagcc ttgtatcaat gagttgaata ccatatcgga agtatatgaa gaatggaaaa
     7081 ccgaaacagg tgtaaagttg gtagccatat caatagacga tgcaagaact atgaatagtg
     7141 tgaagccttt tataaatggt aaaaactggc cttatgaagt ttacttagat cccaacagcg
     7201 actttaaacg cgctatgaat gtaggaccgg ttccacatac ttttttggta aatggtaaag
     7261 gtgagatagt atcgcagcac acctcttttt cgcccggtga tgaagaaaag ttattggaag
     7321 aaataaaaaa gttggtagca ggaaattaat accaattaaa cgaaaaataa aaaaccggtg
     7381 atgagccggt ttttttatgc tggtattttt accttgaagt aaccaatata gtaatacaat
     7441 aaattggaaa acgctttata gcttttctta cacacttttg ccaacagctt tagttatgag
     7501 atagaaaccc tgtaatcttt ttgtgcatat caggaatttt atttttcatc acgtcccaaa
     7561 caattgcata attgaccccc atataatcgt gaatcagtct gtcacgcata ccggcaatat
     7621 ttttccattg aatagcgctc catgttagtt taaagtcggc aggtattttc tttgtagctt
     7681 ctccaataat ttctaagctt ctaacaactg ctcgcttcaa agtttcgtct acaataaaat
     7741 tttcaaacgt taagttatca cttacagaga ttatgtatga acactcatcc tgaatatgtt
     7801 tgagatatgt tgttggctct ttagacatat tttacctcat tcaaaatatt agggccaata
     7861 tatggactca agccattttt ggttacaact tcgacttttt cgtttttgaa gatattttcc
     7921 agtaaatcat aaactgccat aaagttgtca aagttttcct tttctggttc gaaatcaatc
     7981 aacaaatcaa tatcgctatc gtttgattgc tcattacgta tgtacgatcc aaataaacct
     8041 acattacgaa tacccaattt agaaagcata tgcatattgg catgcagctt ttttaatatg
     8101 tcgtcttttg ttgtcattca aattttttca aatatacaaa agtttgtagt ttgaattgtg
     8161 tgtgaacaat tgagcaaaat ttattttcgg aaatgaactc ctgcatgtcg gcttgctaaa
     8221 cctccttgcg catttgcgga aaaaataaaa cgtcttgtat agatggctga ttagtcatta
     8281 tcattgccaa cctatcaata ccaattccta atcctgcagt tggtggcata ccgtattcta
     8341 tagcgcgcaa aaaatcttcg tcaagttgca tcgcttcttc atcgccacgt ttgcctagtt
     8401 cgagttgctc ttcaaaacgt tcgcgttgat ctatagggtc gtttaattcg ctaaaggcat
     8461 tgcatatttc tttaccgttg catacagctt caaaccgttc aacaaggccg ggcttggagc
     8521 gatgtttttt tgcaagcgga ctcatttcaa caggataatc tgttataaat gttggttgta
     8581 ttaattgatg ttcaaccgtt tcgccaaata tttcgtctat caacttaccg cgccccatcg
     8641 aattatcggc atgtatatta agcttggcac atacctcacg catttgggct tcatccattt
     8701 ccgaaatatc gattcctgta taatgttgta tggcttcgta catcgtatat cgcttccatg
     8761 ggcgtttaaa atcgataatg ttttcgccca cttttacttc tgttgttttg tgcaattcaa
     8821 gagcaatttt ttctatcact tcttctacca gattcatcat ccagttgtaa tctttatagg
     8881 caacatacaa ttcaatttgc gtaaactccg gattgtgaaa gcgactcata ccttcgttac
     8941 gaaaatcttt cgaaaattcg tatacaccgt caaacccgcc aacaatcaac cttttgaggt
     9001 ataactcatt tgctatgcgc aaatataaag tcatatcgag cgtattgtga tgcgttttga
     9061 aaggccttgc tgctgcacca ccataaagcg gctgtaatat tggcgtttct acttcgaggt
     9121 atcctttttc attaaggtaa ttacgcatta cattcataag ctgcgtacgc ttgcgaaata
     9181 catcgcgtac cgaagggttc acaattaaat caacataacg ttggcgataa cgcaattccg
     9241 aatcggtaac ctcatcatat ttctcaccat ctttttcttt tataattggc aaagggcgta
     9301 acgatttggt aagcaatttt atc
//
LOCUS       DAORQA010000058         9249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1959_length_9249_cov_22.995106, whole genome shotgun sequence.
ACCESSION   DAORQA010000058 DAORQA010000000
VERSION     DAORQA010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9249
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1959_length_9249_cov_22.995106"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..764
                     /locus_tag="PK736_04950"
     CDS             165..764
                     /locus_tag="PK736_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRC32771.1"
                     /translation="MPKVKIARKSIHIDMTAMTDVAFLLLTFFMLTTKFKPDEPFIVD
                     MPSSISEIMLPDADIMQIAVEKEGEIFFTMDGQEVRANLLRKMGEQYQIKFTEKQITQ
                     FALLESFGLPIAQLPQFLDLKAEQRVSVKQTGIPCDTVKNELRDWIWFGRLSNPKVRI
                     AIKGDRETHYPVVKRIMDTLQERKVNRFNFITNLESGSN"
     gene            813..1448
                     /locus_tag="PK736_04955"
     CDS             813..1448
                     /locus_tag="PK736_04955"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRC32772.1"
                     /translation="MSQIEEGDGGGHKKGESKAKPKKKTILIDFTPMVDLGFLLITFF
                     MLTTTMSKPKTMEINMPLKEDVPPEDRTKFKESQTMTVILGENNVVYYYFGINNPTLD
                     STNFGKDGIRKVLLLENAKRNPLVDSVNIYKNMIVNRLMDKDEAKANISRIKAYKDAL
                     IVVIKAKDESNYANLIDILDEMSICNIGRYAVVDITPQDLQLLSGTLPVTD"
     gene            1461..2327
                     /locus_tag="PK736_04960"
     CDS             1461..2327
                     /locus_tag="PK736_04960"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRC32773.1"
                     /translation="MILFIVILILIAFILVDVFTTKVSLYDKLWTDNVFAGKNKNYGA
                     YYLRSLGGKHVMRGTLISVILFSVGLGAPIIAKYLGNFADGMKDKVTEVTTLEAPPPI
                     DKTEPPPPPPEAPPPPPLKSTVKFVAPEIVKDEEVKDEPPPTVEELKDVDVGKKTEEG
                     DPGGVDNSLIDEGPAVIEETPQIFTIVEQMPEFPGGEESLIKFIQSNFRYPAMERENN
                     IQGTVYLTFVVRPDGEIADIKVMRGVAGGPNLEKEAIRLVKAMPKWKAGKQNGKQVSV
                     QFNLPVKCQLKG"
     gene            2507..2716
                     /locus_tag="PK736_04965"
     CDS             2507..2716
                     /locus_tag="PK736_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32774.1"
                     /translation="MNKKYFFYFSLVMCVAYVAFGIFIIFFDTAIQVNKSMKVFFGVA
                     IILYGIFRSIRSYQLVRGKSNNELK"
     gene            2719..3660
                     /locus_tag="PK736_04970"
     CDS             2719..3660
                     /locus_tag="PK736_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRC32775.1"
                     /translation="MSKIIYLSVCSLLLLLLCACNPYPNQKQYDDTTTSGNIKIACDQ
                     TFYNIIDGELDTFHALYKYAHIEPTYCSENEAFKSLLIDSSRIIIVSRMLNNEELEKF
                     KSEKITPKQLHVATDAVAFIVNENNVDSVFTPTQLQQILSGEILDWNQISKQNKSGQM
                     LLVFDDVYSSTARYAKEIINSGKEIKGNVFAVNKSEEVIAYVKSHPNAMGVVGVSYIS
                     DGDDPSTKTFLKNIKVVAVKNETDGTRYKPYQAYVAQGLYPYTRKVYIISREARTGLG
                     TGFSSFVAGDVGQKIILKMGVLPASMPVRIVGFRDNN"
     gene            3800..5380
                     /locus_tag="PK736_04975"
     CDS             3800..5380
                     /locus_tag="PK736_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32776.1"
                     /translation="MKKLFLSILVLGAYFAHAQAPTIESGLAALENDQFNKAKGIFMT
                     LLNSNPETSGQAYYYLASAYYQSGKSDSGQMIINKATSLLPQNPYIKVAAGREALNAK
                     NPVDAKANFDAAIQSTKGKDAKVFALIGDAYLNSAMPDRAAAITYLQQGIIVDKTNTE
                     LYLLYGDAKKRMENGGGEAASAYEKAVELKSVARGQTRLGSLYGEARNLETSSAAFDA
                     ALVADASYIAANRDKGEMLYTLKKYSLARDEYKTYMETADTTNDNLTRFAYMSFQAKD
                     YESVSSIINILLAKDSTNVTLYRLNGYSFCEKKNYPAALENLQKFFDKADTSKIIYLD
                     YKYYGKALMESKQDSLAEIMIGKAVILDSTECDLYADQGDIYMRLKRYTDAQASYAKK
                     MACANSQGAADYFLLGKSYYFNKQYDISDSLFRKVIELKPASSVGYYWAARSTLYKKE
                     NNIDEAIPLYDKYLEIVSVDLTKASKKELAEAYLNKGKNAILKDDKVKAKEFLNKVLE
                     FDAENKEAKDLLSSLAKG"
     gene            complement(5562..6722)
                     /locus_tag="PK736_04980"
     CDS             complement(5562..6722)
                     /locus_tag="PK736_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32777.1"
                     /translation="MDHFKLLIILLTLSLSIRGQVRVNLTFEDDTDSSFISDAFVLDI
                     TNGRTYQTNSKGMIDLILGEMPSQIKYTIRHLNYVCDTFVFTPSSAVTYTVYVKPKFF
                     QISPVDVSSQSAKEVMQRALQQIESLYNKEKANSHYFYRQYHYENGHEVRWIECDVVL
                     EKYPVINRSQNLNYAKMYVSNLKKTKVTEQNQDEHGDHLADLLNQIPTINSIGTVCNY
                     KAIDSYNFVFDIENETADLYAINFQSKSALGDKLEMGNIIINKSDFAFLKIETQHYPT
                     KQYYNNLIFGHSNKFVWSMKNESTIFTFSKYRNSYSLISCFKTYTHDLIYSDTKQKAF
                     VVTENFELFVSKSGSINTPVSKKSFSQFTNLYTSHGEDLVIPNANYPCVFCK"
     gene            complement(6841..>9249)
                     /locus_tag="PK736_04985"
     CDS             complement(6841..>9249)
                     /locus_tag="PK736_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRC32778.1"
                     /translation="PTQDGRTAPKVSAVGSDVMSTLPPNTYGPNSGTSMSCPAVSGTL
                     AQLNQCYKDNNANVNPPSALIKAIMMNSCDDLGNPGPDFVFGYGRINAYRAAIDIENA
                     QYFNGTLSQGDSTEFTLTVPSNVGQLKVMLYWHDYEAAIAAAPALVNDLDLKVVYNSF
                     NTYLPLVPDPTPVLANLSANAVPNVDHMNNEEQVYVNLPSPGTYTFKVSGFNVPQGAQ
                     DFYVVYDYVYDEITVTYPLGGESMEPNELQKIRWDAYGNAGTFTVSYSTDSGATYNVI
                     SSTVAGNRRYLDWTTPSVQTGKCLVKVERNGLLDVSEAPFSIMDVPTNLQIAFACPDS
                     IKLTWNAVANAVFYEASVLGAQYMDSMGTNVNTEYVYTGLNPFAVNWFSVKAYGPNDA
                     VGRRAIAISSVPGISNCVVAVDAGISAVSPSGGNLYSCASSGNVAVTADIKNEGLSPI
                     TNFDVAYILDNGSPVVETFTGTINPGAMATHTFTNPINISAPGAHSILAYVVLTNDGN
                     GFNDTLAASVTMINAPAAIGLPYIEDFETFAPCGTDFDCEVTICFLGNDLVNEENLVS
                     DDIDWRTNAGPTPSQGTGPDVDHTTGTAIGQYVYTEASNGCFGKIANMVTPCINLTGT
                     TNPVLSFYYHMFGADMGDLHIDIFANDSWINDVVPAISGNQPNQWILQNVGLSNYIGQ
                     IVNIRFRGVTGPDFASDLALDDIGVAELTGINDDIIQASNFNLNPNPTNGEFQLSLAG
                     AQRGLYDLSVYDATGKLVFAQSINNQNTLMVHQINLTKVDKGVYHLRLIHNGTIYNET
                     LVKY"
BASE COUNT         3046 a         1645 c         1737 g         2821 t
ORIGIN      
        1 tgtatacgta tgatctttgg gtgtgagtag attataaata aatatttttg ctggctgaca
       61 aagggtaaaa gtttttgttt ctataatatt taatgtatgc agaggatagt ttttgacatt
      121 gcgtatgaaa ttatttttaa tgattttaaa gaaacaataa aagaatgcca aaagtgaaaa
      181 tagcgcgaaa aagtatacac atcgacatga cagccatgac cgatgtagct ttcttgttgt
      241 tgactttctt tatgttaaca accaagttta aacccgatga accattcatt gtagatatgc
      301 catcttctat ttcggaaata atgctgcccg atgctgatat aatgcagata gcagtagaaa
      361 aagaaggcga aatatttttc accatggatg gtcaggaagt acgggcaaat ttattgcgaa
      421 aaatgggtga gcaataccag ataaaattta ccgaaaagca aattacacag tttgcacttt
      481 tagaaagttt tggtttgcca attgcacagt taccgcagtt tctcgacctt aaagccgaac
      541 aaagagtgag cgtaaagcaa accggtatcc catgcgatac agttaaaaac gaattgcgtg
      601 attggatttg gtttggccga ctgtcaaatc ctaaagttcg catagctatt aaaggcgacc
      661 gcgaaacgca ttaccccgtg gtgaaacgta taatggatac gctgcaggaa cgaaaagtga
      721 atcgtttcaa ttttattacc aatcttgaga gtggtagtaa ttaatttttt atggaatttt
      781 taattaaaac tatcaaacta aaaaaataag aaatgtcaca aatagaagaa ggcgatggtg
      841 gcggtcacaa aaagggcgaa agcaaagcca aacctaagaa gaaaacaata ttaattgatt
      901 ttactccgat ggttgacttg ggatttttgc ttattacttt ttttatgctt acaacaacaa
      961 tgagtaagcc taaaacaatg gagataaata tgcccttgaa agaagacgtt cctccggaag
     1021 atcgcaccaa gttcaaagaa tcgcaaacaa tgacagttat ccttggcgaa aataatgtgg
     1081 tatactatta ttttggaatt aacaatccaa ctttggattc aaccaatttt ggaaaggatg
     1141 gaattagaaa ggtactgttg cttgaaaatg ccaaacgaaa tcccttggtc gattcagtga
     1201 acatttacaa aaacatgatt gtaaacaggt tgatggataa ggatgaagca aaagcaaata
     1261 tatctcgcat aaaagcatac aaggacgcat taattgtggt aataaaagcc aaagatgaat
     1321 ccaactatgc taatctaata gatatacttg acgaaatgag catttgtaat attggccgct
     1381 atgcagttgt tgatattact ccacaagatt tgcaactact cagtggaact ttacctgtaa
     1441 ccgattaaaa tattataatt atgattttat ttattgttat tctcatatta atagcattta
     1501 ttttggttga tgtttttaca actaaagtaa gtttgtacga caaactgtgg accgacaatg
     1561 tatttgccgg caaaaacaaa aactatggcg cgtattattt gcgctcgttg ggcggcaagc
     1621 atgttatgcg tggcactcta atatcagtaa tacttttttc tgttggattg ggcgctccta
     1681 ttattgcaaa atacttaggc aattttgctg atggaatgaa agataaagtt accgaagtaa
     1741 ctacactcga agcaccaccg ccaattgata aaaccgaacc gccaccacca ccacccgagg
     1801 caccgccacc accaccatta aagtcgactg taaaatttgt ggcacccgaa attgtgaaag
     1861 acgaagaggt gaaagatgaa ccaccaccaa cagtagaaga acttaaagat gttgatgtgg
     1921 gtaagaaaac agaggaaggc gatcccggtg gtgttgataa cagtttaata gatgaaggtc
     1981 cggcagtaat tgaagaaaca ccacagatat ttacaattgt agaacaaatg cccgaatttc
     2041 cgggtggaga agagtcgttg ataaagttta ttcaaagtaa tttccgttat cccgctatgg
     2101 agcgcgaaaa caacatacaa ggaactgtgt atcttacatt tgtagtaaga cctgatggtg
     2161 aaattgccga tattaaagtg atgcgcggag ttgcaggtgg ccccaatctt gaaaaagaag
     2221 ctatccgatt agtaaaggca atgcctaaat ggaaagccgg aaaacagaat ggtaaacaag
     2281 tttcggtgca atttaatctt cctgtaaaat gccagttgaa gggataagct caactattta
     2341 aattaaagca aacccaagaa gcattaaatg tatcttgggt ttgttgcatt aatacggtta
     2401 acatttttgc acaaaacata taagtctctt tcaaaaaaaa tagaaatatt atgcttatca
     2461 aactcaactt gacgattcaa aataaattga ataactttca taaaaaatga acaaaaaata
     2521 tttcttctat ttctcgttgg taatgtgtgt tgcatatgtt gcatttggaa tttttattat
     2581 ttttttcgac acagccattc aagtaaacaa atcgatgaaa gttttttttg gtgtggcaat
     2641 tattctttat ggcatatttc gttctatacg atcttatcag ttggtgagag gcaaatccaa
     2701 taatgaatta aaataaaaat gagtaagatt atttatttat ccgtttgcag tttgttgcta
     2761 ttactattat gtgcatgcaa cccatatcca aatcaaaaac agtatgatga taccaccaca
     2821 tcgggcaata ttaaaatagc ctgcgaccaa actttttata atatcattga tggtgaactc
     2881 gatacatttc atgcgcttta taaatatgca catatagaac ctacctattg ctcagagaat
     2941 gaagccttta aaagtttgct catcgatagt tcgcgcatta ttattgtttc gcgaatgttg
     3001 aacaacgaag agctcgaaaa attcaaatcc gaaaaaatta cacccaaaca gttgcatgta
     3061 gcaaccgatg ctgttgcatt tattgtgaat gaaaacaatg tcgattctgt attcacacct
     3121 acacaactgc aacaaatttt aagtggagaa atacttgact ggaatcaaat ttcgaagcaa
     3181 aataaatcgg gacaaatgct attagtattt gacgatgttt attcgagtac agcacgatat
     3241 gcaaaagaaa ttattaatag tggtaaggag ataaaaggta atgtttttgc ggtcaacaaa
     3301 agtgaagaag taatagcata tgtaaagtcg catcccaatg caatgggagt tgtaggggtc
     3361 agttatataa gtgatggtga cgatccgtca acaaaaacat ttttgaaaaa cattaaagta
     3421 gtggccgtaa aaaatgaaac agacggcaca cgttacaagc cttatcaagc ctatgttgca
     3481 caaggtttat atccctacac ccgaaaggta tatattataa gccgtgaggc gcgtacaggt
     3541 ttgggtacag gttttagtag ttttgttgcc ggtgatgtag gtcaaaaaat catacttaaa
     3601 atgggagtgc tgccagcatc gatgccggta agaatagttg gctttagaga caataattaa
     3661 acttgctgat aacacatccg gcattatcaa aacatttata tttttgcgcg ctcaaaattc
     3721 caattcactt atgcttcact cgtataaatg atatggttat aaaattgtat attcatattt
     3781 cataataatc aataaaaaga tgaaaaagtt atttctaagt attcttgttt taggggcata
     3841 ttttgcacat gctcaggcac caactatcga aagcggatta gcggcactgg agaatgatca
     3901 attcaataaa gcaaaaggca tattcatgac gttgctaaat tcgaatcccg aaacatccgg
     3961 tcaggcttat tactatttag cctcagctta ttatcaatcg ggcaagagcg attccggtca
     4021 aatgataatt aataaggcga catcactgtt acctcaaaat ccatacataa aagtagctgc
     4081 cggtcgcgaa gcattaaatg ctaaaaaccc tgtggatgca aaagcaaatt tcgatgctgc
     4141 tatacagtca acaaaaggaa aggatgccaa agtttttgcg cttattggcg atgcttattt
     4201 aaacagtgca atgcccgatc gtgctgcagc aatcacgtat ttacagcaag gaataattgt
     4261 cgataaaacc aacacggaat tgtatttgct ttatggcgat gcgaaaaaga gaatggaaaa
     4321 cggtggaggc gaagcagcat ctgcatacga aaaggctgtg gaattaaaat cggtagcacg
     4381 cggtcaaaca cgtttgggaa gtttatatgg tgaagcccgt aatttagaaa cctcttctgc
     4441 tgccttcgat gcggccttag ttgccgatgc gtcttatatt gctgccaatc gcgataaagg
     4501 tgaaatgcta tatactttaa aaaaatattc tctggcacgc gatgaataca aaacatacat
     4561 ggaaacagct gataccacaa atgataatct tacccgattc gcatacatgt cgtttcaagc
     4621 caaggattat gaaagtgtaa gcagtatcat caatatttta ttagcgaaag attcgaccaa
     4681 tgttaccctt taccgattga acggatattc attttgtgaa aagaaaaatt atcctgctgc
     4741 cttagaaaat ttgcaaaagt tttttgataa agctgataca tcaaaaataa tatacctcga
     4801 ttacaaatat tatggaaaag ctttgatgga aagcaagcaa gattctttag ccgaaataat
     4861 gattggtaaa gcggttatac ttgattctac agagtgtgat ttgtatgccg atcagggcga
     4921 tatttatatg cgcttgaagc gctacaccga tgcacaggct tcgtatgcaa agaaaatggc
     4981 atgtgccaat tcgcaaggtg cggcagatta tttcttactt ggtaaatctt attatttcaa
     5041 caagcaatat gatatttccg attcgttgtt ccgaaaagtt atcgaattga aacctgcctc
     5101 aagcgttggt tattattggg cagcacgttc tactttgtat aaaaaggaaa acaatattga
     5161 cgaagccatt ccattgtacg ataagtacct tgagatagtt tctgtggatt taacaaaagc
     5221 atctaaaaag gagttggccg aagcgtatct aaataaagga aagaatgcca tcttgaaaga
     5281 tgataaagta aaagcaaaag aatttttaaa caaagttttg gaattcgatg ccgaaaataa
     5341 agaagcaaaa gaccttttaa gcagtttggc taaaggataa atttaaatcc cctgaaataa
     5401 acttcagggg attttttttg agcttttttt atcaatcact tttttgtatt actgcagcac
     5461 aaaattatta cccttctgaa aaagcgatga gtttcaatta tgatagtgcc gtattcaccc
     5521 tagatggaat aagttattgt aatcccaatt acctaagcac actatttgca aaacacgcac
     5581 gggtaatttg catttggtat aactaaatct tctccatgtg acgtataaag gttggtgaat
     5641 tgtgaaaagc tctttttgga aacaggggtg ttgatggaac ccgattttga aacgaacaat
     5701 tcaaaatttt cagtcactac aaatgctttt tgtttggtgt cactataaat caaatcatgc
     5761 gtgtatgttt taaagcatga aatcagcgag taactatttc tgtatttgct aaaagtgaat
     5821 attgtgcttt catttttcat tgaccatacg aatttattgg aatggccgaa tattaggtta
     5881 ttatagtatt gtttggttgg gtaatgttgc gtttcgattt ttaaaaatgc gaaatcactt
     5941 ttgtttatga tgatattgcc catttccagt ttatcgccca aagccgattt actctgaaaa
     6001 tttattgcat acaaatcagc tgtttcgttt tcaatatcaa aaacaaagtt gtaagaatct
     6061 atggctttat aattacaaac agtaccaatt gaattaatag taggaatttg atttagtaaa
     6121 tcggcaagat ggtcgccatg ttcgtcttgg ttttgttcgg ttacttttgt ttttttcaaa
     6181 ttcgaaacat acatttttgc atagttcaaa ttttgacttc tatttatcac aggatatttt
     6241 tccaatacta catcacattc tatccatctc acttcatgtc cattttcata atggtattgt
     6301 ctgtaaaaat aatgagaatt ggctttttct ttattgtaca aactttctat ttgctgcaat
     6361 gcacgttgca ttacttcctt tgccgattga ctcgatacat caacagggct tatttgaaag
     6421 aatttgggtt taacgtatac ggtataggtt acagcacttg atggtgtgaa aacaaatgta
     6481 tcgcaaacgt aatttaaatg ccttattgta tattttattt gtgatggcat ttcaccaaga
     6541 attaaatcta tcattccttt gctgtttgtt tgataggttc tcccattggt aatgtcaagc
     6601 acaaacgcat cagatataaa tgacgagtct gtatcatctt caaacgtcag atttacacga
     6661 acttgtcctc tgattgataa agacaaagtc agcagtatta ttagtagttt gaaatgatcc
     6721 attttatttt ttaaaagttg ataaaaattt gtctgaacta catatttaga aatgtaattt
     6781 ttaggtaaaa aaaaacaggg caactgcggc cctgtctttt attaaaaact gattttgaaa
     6841 ttagtattta actaaagtct cattatatat tgttccatta tgtatcaaac gcaaatgata
     6901 tactccttta tctacttttg tcaagtttat ttgatgtacc attaaagtat tttgattgtt
     6961 aatactttgg gcaaaaacaa gtttacccgt tgcatcatat acactcaaat catacaatcc
     7021 gcgctgtgca ccggccaacg ataattggaa ttcgccattg gtgggatttg gatttagatt
     7081 aaaattgctt gcttgaataa tatcatcatt aattcctgtt aactcggcaa cgccaatatc
     7141 gtcaagtgct aaatcgcttg caaaatcagg acctgtaact ccacgaaaac gtatgtttac
     7201 aatctggcca atataattgc tcaaaccaac attttgcaaa atccattggt taggttggtt
     7261 acccgatatt gcaggcacaa catcatttat ccaactatcg tttgcaaata tatctatgtg
     7321 caaatcgccc atatcagcgc caaacatatg atagtaaaaa ctcaataccg ggtttgtagt
     7381 tccggtaagg ttaatacaag gagttaccat attagcaatt tttccaaaac aaccgtttga
     7441 tgcctcggtg taaacatatt gacctattgc tgttccggtt gtatgatcta catcgggacc
     7501 ggttccttgc gatggagtag ggcctgcatt tgtacgccag tcaatatcat ccgaaaccaa
     7561 gttttcctca ttcactaaat cgttacccag aaaacaaatg gttacctcgc aatcaaagtc
     7621 tgttccgcaa ggtgcaaatg tttcgaaatc ttcgatataa ggaagcccaa ttgctgccgg
     7681 ggcatttatc attgttaccg aagcagccaa tgtatcgttg aaaccgttgc catcgtttgt
     7741 taataccaca taagccaaaa tactatgtgc acccggagca ctaatgttta ttggattggt
     7801 aaacgtatgt gtagccattg cgcctggatt tattgttccg gtgaatgttt ccactactgg
     7861 agaaccatta tctaagatat aagccacatc aaaatttgta attggactta agccctcatt
     7921 tttaatatca gcagttaccg caacatttcc tgacgaagca caagaatata aatttcctcc
     7981 tgatggcgat accgctgaaa ttccggcatc taccgcaaca acacaattac ttatgcccgg
     8041 cacactgctt attgctatag ctctgcggcc aacagcatca tttggtccat acgcttttac
     8101 actaaaccaa tttaccgcaa atggatttaa tccggtataa acatattccg tatttacatt
     8161 ggtacccata gaatccatat attgagcacc caaaacactc gcttcataaa acacagcatt
     8221 tgctaccgca ttccaagtca gttttattga atcggggcag gcaaatgcta tttgcaaatt
     8281 cgttggtaca tccataatac taaaaggcgc ctcgcttaca tccaacaaac cgttacgctc
     8341 cacttttacc aaacattttc ctgtttgtac tgatggtgtt gtccagtcta aataacgcct
     8401 attgcctgcc actgttgaag aaataacatt gtaagtggct ccactatcgg tagaatatga
     8461 aactgtgaaa gtaccggcat ttccatatgc atcccaacga atcttttgca attcatttgg
     8521 ctccatactt tcgccaccaa gcggataagt aactgtaatt tcatcatata cataatcata
     8581 aacaacataa aaatcctgcg caccctgagg aacattaaat cctgaaactt taaaagtgta
     8641 agtaccgggg ctaggaaggt ttacataaac ttgctcttca ttattcatgt ggtcaacatt
     8701 gggtactgca tttgcgctca aattggctaa aacaggagtt ggatcaggta ctagcggtag
     8761 gtaagtattg aaagaattgt atacaacctt taagtctaaa tcgtttacta gggcaggtgc
     8821 tgctgcaatg gctgcttcgt aatcgtgcca ataaagcatt acctttaatt gtccaacatt
     8881 ggaaggaact gtaagagtaa actcagtaga gtcgccttgc gataaagtac cattaaagta
     8941 ttgagcattt tcgatatcga ttgctgcacg gtatgcgttt atacggccat aaccaaatac
     9001 aaaatcaggt ccggggttac ccaagtcatc gcatgaattc atcattatgg cttttatcaa
     9061 tgccgatgga ggattcacat ttgcattatt gtctttgtag cattgattca attgtgccag
     9121 cgttcccgat acagcaggac acgacataga tgtgcctgaa tttgggccat aagtatttgg
     9181 tggtagtgtt gacataacat cgctaccaac cgcactcact tttggtgctg tacgtccatc
     9241 ctgggttgg
//
LOCUS       DAORQA010000059         9174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_1994_length_9174_cov_18.824542, whole genome shotgun sequence.
ACCESSION   DAORQA010000059 DAORQA010000000
VERSION     DAORQA010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9174
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1994_length_9174_cov_18.824542"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PK736_04990"
     CDS             <1..852
                     /locus_tag="PK736_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRC32779.1"
                     /translation="KQNAERDLTIARLKKDTTDLSTSMGRLNNLYNDLTKSYDKLVTN
                     EEKIARSKDEETRRALTDLDKTRSDLLRKEDALKLLEKEVTEKQKNLENSQLALTDAD
                     KKLKEKEARVVELQEALARKDSIVSALKTKVTDALVGYNNNGLTITQKNGKVYVSLEE
                     RLLFASGSTTVDKKGIDALKQLSKVLEENPDINVLIEGHTDNVPISGGNIKDNWDLSV
                     LRSTSVVRILIKESKIDPLRLTPAGKGEYNPVDIGSSAEARKKNRRIEVILTPKLDEI
                     FKMMGTN"
     gene            872..1609
                     /locus_tag="PK736_04995"
     CDS             872..1609
                     /locus_tag="PK736_04995"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRC32780.1"
                     /translation="MQKFHKTIFTFLFSIIASSIMSQTIDLQQIKQTKKISVTGSSEM
                     EVEPDQIFITIDLREYYNKDKKKIDIERIKKEFIEACKNAAVPDSDIRLQNVNGYNVA
                     SIWERKKRKDPDFFATVSYIIKLKSTQTMDQLIARLNDEATVGLNVTKLSNSKEAEYR
                     KTVKEDALKNAKEKARYLCESIGERLGGAIFIEEINNDNYYQPMMGKAMMMSNAAMDG
                     GGNIETTSTDFQKIKIRFEIRAEFEIK"
     gene            1861..2865
                     /locus_tag="PK736_05000"
     CDS             1861..2865
                     /locus_tag="PK736_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRC32781.1"
                     /translation="MKIFITGAAGFLGSHLADRMLELGHEVIGCDNYIGGYQDNVNHK
                     MEFHHADCMDLAKMRELTKGCEIVYHCASTAYEGLSVFSPHFVTMNTYQITMSVLSAS
                     IENRVRRFVYCSSMARYGFLPVVPFTEEMTPMPLDPYGIAKLASENTIVNLAKVHNFE
                     YAIAVPHNIIGARQKYDDPFRNVAAIMINLMLQGRQPIIYGDGEQKRNFSFVQDCIYC
                     LEKMAFQDNVVGEVINIGPDDEFVTINELAQVIGKLLNMEVKPDYYPDRPMEVKHASC
                     SADKARRLLNYKPNMTLEAGLTEMINYIKKRGVLPFEYHLDVEIKNDKTPKTWTNRLF
                     "
     gene            3056..3421
                     /locus_tag="PK736_05005"
     CDS             3056..3421
                     /locus_tag="PK736_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRC32782.1"
                     /translation="MKIIQQNKQIALDWIAAFNTHDVDAILKLYDDNAIHFSPKLKVR
                     EPQTNGFVIGKIQMKQWWDDAFTRLPSLNYELINLIVDENNLLMEYKRTVDGEADMMV
                     AEVLEIQNGLIVKSRVYHA"
     gene            3482..4525
                     /gene="rlmN"
                     /locus_tag="PK736_05010"
     CDS             3482..4525
                     /gene="rlmN"
                     /locus_tag="PK736_05010"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019942116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRC32783.1"
                     /translation="MHHKQDIRALNYKQLEEFFLAHGEKKYRAKELTNWLYKKSVVNF
                     AEMTDLKSGLVEFLESHFYINPITIEKKQVSDDRTIKNAFKLHDGNIVEGVLIPSDNR
                     VTACVSSQVGCSLTCKFCATGKLDRIRNLEVGEIYDQVAIIKQQSETHYNMPLSNVVF
                     MGMGEPLLNYNNVLQAIEQLTSGQGLNMAPRRITVSTAGIAKMIKKLGDDEVNFNLAL
                     SLHASNDKKRENIMPINEHNNLDALIKSLQYFYAKTKSVVMLEYIVFNNFNDELQDAE
                     ELFQFAKQVPCKVNLIEYNSIGDSIYKNTSEEKLEAFQQYLYERDVFVTRRRSRGKDI
                     DAACGQLANKNLA"
     gene            complement(4555..6849)
                     /locus_tag="PK736_05015"
     CDS             complement(4555..6849)
                     /locus_tag="PK736_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32784.1"
                     /translation="MKKHFYLFTMLIAMMSGNIQAQKYANTWYFGIQAGLDFNGGAPV
                     AINNSAMSTAEGCSSISDDNGAILFYTDGVSVWNKNHVLMANGTGLGGGSSSTQSACI
                     VKQPGVNTNYYIFTSGEFNSTAGFQYNIVDISTNGGLGSVILKNQILYTPTTERVTAH
                     THSNGNDIWILSHQSLGSEFVAYLLTSTGLNLVPVITSVGPVFNGSGLDFLGNLKFSP
                     DGFKVAMAVSAPDTFYVFDFDPSTGVLSNPISLNSTNSSEGAYSVEFSPSGNVLYATS
                     EIPFKLFQFDLTSGNAVTMNASRLDIPTIPLGGSYIGGLQLGPDGKIYMTRNTCGWLG
                     VINDPNVAGFGCNYADSGIFLNGASCIYGLPNLNQAAYRFITIEKFCFGDATYFGAND
                     SVNYLAFQWNFGDPGSGPLNVSYYATTSHVFTAPGNYTVELIRASLAPPFGDTTYYDI
                     IINPLPVVNLGNDTTICNGQSYIINPGAFSSYLWQDGTTNSTFAATVTGTYTVTVTQQ
                     DCKATDSIVVTVQPCALPLANLSSSDTTFCEKQCIDFFDLTVNNPSSWLWTFNGASPS
                     TSTDQNPAGICYNNYGSFDVQLIACNNLGCDTVLFPSFITEVASPPAPLITQSNDTLF
                     CNTTQVTYAWYNVNNVNLVLGTNNYFVPTIAGSYYVVISDSNDCNVASATFNSNVGYN
                     ELKNDGINIFHNQQAGIVYITNTNLTNAIQEIKLFDLQGKCIEKNTLREQTSQIKLPN
                     SSSVYFVNITTTTKNYMVKIAAIK"
     gene            7054..7812
                     /locus_tag="PK736_05020"
     CDS             7054..7812
                     /locus_tag="PK736_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016193720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRC32785.1"
                     /translation="MEITKDLKLAVLIDADNVPYAHIKEMLEEIAKYGTPTFKRIYAD
                     WTMPTVSGWKSVLLESAITPVQQYGYTKGKNSTDSAMIIDAMDILYSAKVDGFCIVSS
                     DSDFTRLAIRLREAGMKVIGIGEKKTPTSFISACEKFIYIEILKPQIIEEKKVSNGKV
                     TKQLIQKAPSLNKVDHALIKLISDSINDLADENGWAFLGDLGNLLLKKQPDFDPRNYG
                     FTKMLSLIKSIPKFLVDARDAGKNNIKHIFIKIK"
     gene            7812..9098
                     /locus_tag="PK736_05025"
     CDS             7812..9098
                     /locus_tag="PK736_05025"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32786.1"
                     /translation="MKSISAKLCCACLFLCIHANIYSKNISILFIGNSYTYVNNLPEM
                     VRQMGLTMGDTIFVDSSTPGGYTYQLHTTDANTIAKIKQQQWDYVVLQEQSQLPSLSP
                     AFVNVNSVPYALMLDSMIKANNACTKTIFYMTWGRKYGDGSNCASYPPVCTYAGMQQR
                     LKESYLLFSDTCKAVAAPVGIAFKNYIAIDTLLDLYSPDFSHPSLAGSFLAAATIYST
                     ITHNQITNQNYSAGLTVTQAAALQNTSWQTIIDSSALWNLGVNTPFANFTYNTLPNLT
                     VQFICPGNVNYTHFWDFGDTTFSTLAEPVHQYLYSSYFGVKHIVYDSCTYDSAQVITN
                     ITPQNISNITVDESNFAFYNSADETIAMSLWKLSSHNLTISLYDVSGKLILKNNYTPT
                     KPLTYLNVRKISNGMYILNAVDDVTGKYFSQKIIKN"
BASE COUNT         3096 a         1690 c         1770 g         2618 t
ORIGIN      
        1 aagcaaaatg ccgaacgcga tttaaccatt gcccgtctta aaaaagacac cacagacctt
       61 agcacatcta tgggcagact caataatttg tacaacgact taaccaagag ttacgataag
      121 ttggtgacaa atgaagagaa aatagcccgc agtaaggatg aagaaacccg cagagcattg
      181 accgacctcg ataaaaccag aagcgatctt ttgcgaaaag aagatgcatt aaaattgcta
      241 gaaaaagaag ttaccgaaaa gcaaaaaaat cttgagaact ctcaactggc attgaccgat
      301 gccgataaaa aattaaaaga aaaggaagca cgtgtagttg aactacaaga ggcattggca
      361 cgcaaagatt ctatagtgtc ggcattaaaa acaaaagtaa ccgatgcgct ggtgggctac
      421 aacaataatg gccttactat cacccaaaaa aatggtaaag tttatgtttc gctcgaagag
      481 cgtttgctat ttgcatcggg cagcaccaca gtagataaaa aaggaattga tgcgcttaag
      541 caactctcaa aagtgctcga agaaaatcct gatatcaatg tgctgataga aggacatacc
      601 gataatgtac ccataagcgg aggcaacata aaagacaact gggatttgag tgtactgcgc
      661 tctacatcgg tggttcgcat ccttattaaa gaaagcaaaa tcgatccttt acgtttaacc
      721 ccggcaggta aaggcgaata taatcctgtc gacataggat catcggccga ggcgcgtaaa
      781 aaaaatcgca gaatagaagt tattcttact ccaaagctgg atgaaatatt caaaatgatg
      841 ggaaccaatt aatcgcttaa caaaaggcac catgcaaaaa tttcacaaaa caatattcac
      901 atttttattt tcgataatag cttcaagtat catgagccaa accatagact tgcaacaaat
      961 aaaacaaaca aaaaaaataa gcgtaaccgg cagctccgaa atggaagtgg agcccgatca
     1021 aattttcatc actatcgact tgcgcgaata ctacaataag gacaaaaaga aaatagatat
     1081 agaacgcata aaaaaagagt ttatagaagc atgtaaaaat gcggctgttc ccgatagcga
     1141 tattagattg caaaatgtaa atggatataa tgttgccagt atctgggaac gaaaaaaacg
     1201 taaagacccc gatttttttg ccacagtatc ttacatcatt aaattgaaaa gtactcaaac
     1261 aatggatcaa cttattgcac gcctcaacga tgaagcaacc gttggtttaa atgtaacaaa
     1321 gctttccaat tcgaaagagg cagagtaccg caaaactgta aaagaagatg ccctcaagaa
     1381 tgcaaaagaa aaagcgcgat acctttgcga atcgattggc gaaagacttg gtggtgcaat
     1441 atttatagaa gaaataaata acgataatta ctaccaaccc atgatgggca aagccatgat
     1501 gatgtcgaat gcagcaatgg acggaggtgg taatattgaa accacatcaa cggattttca
     1561 aaaaataaaa atccgcttcg aaataagggc agagtttgaa ataaaataaa gtcattccat
     1621 tcttcgggtt tattaatgtt tcacatatta ggccaatggc tctattggtg cggatttcct
     1681 gacttctgaa aaaagcttgt tcgcccaaaa aaacctttta tttaatttgt atgttatgta
     1741 ggggcgtgtt actttcgcac cgcttaaaaa taaatgaata gaaccactgt ctctattatt
     1801 tatcaggtgt tcttggttca attttaaaag gaaattaata aataaaaaat agtttttagt
     1861 atgaagattt ttataacagg tgcagcagga tttttaggaa gccatcttgc cgacagaatg
     1921 ttggaacttg gacatgaggt aataggctgc gataattaca ttggtggtta tcaggataat
     1981 gtaaaccaca aaatggaatt tcaccatgcc gattgtatgg atttggctaa gatgcgtgaa
     2041 cttaccaaag gctgcgaaat agtttatcac tgtgcttcta ctgcttacga aggtttgtcg
     2101 gtgttctctc cgcactttgt aacaatgaac acatatcaga ttacaatgag tgtgcttagt
     2161 gcaagtatcg aaaaccgtgt gcgcagattt gtatattgtt cgtcaatggc acgttatggt
     2221 tttttacctg tagttccttt taccgaagag atgactccaa tgcctcttga cccatatgga
     2281 attgcaaaac ttgcaagcga aaatacaatt gtaaatttag ccaaggtgca taattttgaa
     2341 tatgccattg cagtacctca caacattatt ggcgcacgtc aaaagtacga tgatccattt
     2401 cgcaatgtag ctgccattat gattaacctt atgctacaag gccgccaacc cattatttat
     2461 ggcgatggcg aacaaaaacg taatttctct ttcgtacagg attgtattta ttgcttagag
     2521 aagatggcat ttcaggataa tgttgttggc gaagtaataa acattggtcc cgatgacgag
     2581 tttgtaacca ttaatgaatt agcacaagtg attggcaagt tgcttaatat ggaagtaaaa
     2641 cccgactatt atcccgaccg tccgatggaa gtaaaacatg caagttgcag tgccgataag
     2701 gcccgcagat tattaaacta taaacccaac atgaccctcg aagcaggtct taccgaaatg
     2761 ataaactaca ttaaaaagcg tggtgtgctt cctttcgaat accaccttga tgtagaaatt
     2821 aaaaacgaca aaacgcctaa gacctggact aataggttat tttagtttgg ttattaggta
     2881 attgggttag taagttatta gttaattcaa ttcacattta taaaaaggat gcagcaataa
     2941 ttgctgcatt tttttttgga aggaatatca tttgtgcagc aacaatgtta tttttttgca
     3001 aattgctgga gcaaattttc ttaaaaagac aactttcaaa acacaaattg aagccatgaa
     3061 aatcattcaa caaaacaagc aaattgccct cgattggata gcagcattca acactcacga
     3121 tgtggacgct attttaaaat tgtatgatga taatgctatt cacttcagtc caaaattgaa
     3181 agtgcgcgag ccacaaacca atggttttgt aattggcaaa attcaaatga aacaatggtg
     3241 ggacgatgct tttacgcgtt tgccatcatt gaattatgaa ttgataaatc ttattgtaga
     3301 tgaaaataat ttactcatgg aatacaaacg caccgttgat ggcgaagccg atatgatggt
     3361 ggccgaagtg ttggaaatac agaacgggct tattgtaaag tcaagggttt atcatgctta
     3421 gcaaattgtt tactttgata taaatgctgc gctctcgttc cgataaatta tatttgcaac
     3481 catgcatcac aaacaagaca tcagggcatt aaactacaaa cagcttgaag agttttttct
     3541 tgcgcacgga gaaaaaaaat accgtgcaaa agaacttacc aattggttgt ataaaaaatc
     3601 tgttgtcaac tttgcggaaa tgactgacct taaatccggg ttagtagagt ttttagagtc
     3661 gcacttttat atcaatccaa tcaccataga aaaaaaacag gtgagtgacg atcgcaccat
     3721 aaaaaacgcc tttaaacttc acgatggaaa tattgtagaa ggtgtattga taccaagtga
     3781 taatagagtg actgcatgtg tatcgtcaca ggttggttgc agtcttacgt gtaaattttg
     3841 tgctacagga aaactcgatc gcattcgcaa tttagaggtg ggcgaaattt acgatcaggt
     3901 tgcaattata aaacaacaat ccgaaacgca ttacaatatg ccgcttagta atgttgtgtt
     3961 tatgggcatg ggcgagccac ttttaaatta taataatgtg ttgcaagcca tagaacaact
     4021 tacttcgggg caagggttaa acatggcacc aagacgcatt accgtttcaa ctgcaggcat
     4081 tgctaagatg attaaaaaac ttggcgatga tgaagtgaat ttcaatctcg ctttatcgct
     4141 gcatgcctcc aacgataaaa agcgcgaaaa cattatgccg ataaatgagc ataataatct
     4201 tgacgccctt ataaaatcat tacaatattt ttatgcaaaa acaaaatcgg tggtgatgct
     4261 tgagtatatt gtattcaata attttaatga tgaattgcaa gatgccgagg aactttttca
     4321 atttgcaaaa caagtgccct gcaaggtgaa tttaatagag tacaattcta ttggcgatag
     4381 catttataaa aacacaagcg aagaaaaact cgaagcgttt caacagtatt tatatgagcg
     4441 cgatgtgttt gtaaccagac gcagaagccg cggcaaagac attgatgctg cttgcggaca
     4501 attggcaaat aagaatttgg cgtgatgaac gctgcaactg ttttcgattt acatttattt
     4561 tatcgcagca atttttacca tataattttt tgtagtggtg gttatgttta caaaataaac
     4621 actggaagaa tttggcaatt tgatttgtga tgtttgttcg cgtagggtat ttttttcgat
     4681 gcattttccc tgtaaatcaa acagctttat ttcctgaatt gcatttgtca aatttgtatt
     4741 tgtgatgtat acaatgccgg cttgttgatt gtgaaaaata ttaatcccat catttttcaa
     4801 ttcgttgtaa cccacattgc tattgaatgt agctgaggca acattacaat cgttactatc
     4861 tgaaataaca acataataac tgcccgctat tgtgggcaca aaataattat tggtccccaa
     4921 aacaaggttt acattattca cattatacca tgcataagtt acttgagtag tattgcaaaa
     4981 taaagtatcg ttcgattgtg ttattaatgg tgcgggtggc gatgctactt cggtaataaa
     5041 cgatggaaac aaaacggtat cgcaaccgag attattacat gcaatcaatt gcacatcgaa
     5101 cgaaccataa ttattgtagc aaattcccgc agggttttga tcggtagaag tgcttggcga
     5161 tgcaccattg aaagtccaaa gccatgaaga aggattatta acagtaaggt cgaaaaagtc
     5221 gatgcactgc ttttcgcaaa atgtagtatc gcttgatgat agatttgcca aaggcaatgc
     5281 acagggctgt actgtcacca caatggagtc ggtagcttta caatcctgct gggtaacggt
     5341 aacagtatat gtaccagtaa cagtagcagc gaatgtgctg ttagtggttc catcttgcca
     5401 aaggtaactg ctaaaagccc cgggatttat aatatatgat tggccgttgc atatagtagt
     5461 atcgttacca aggtttacta ccggtaatgg attgataatg atgtcgtaat aagtggtatc
     5521 gccaaatggc ggggcaagcg aggcgcgaat aagttcaaca gtatagttgc ccggagcagt
     5581 aaaaacatga gatgtggtag cataatatga cacattcaat gggcccgaac cggggtcgcc
     5641 aaagttccac tgaaaggcta aataatttac cgaatcgttg gcgccaaaat aggtagcatc
     5701 gccaaagcaa aatttttcaa ttgttataaa acggtatgcc gcctgattca aattaggcaa
     5761 accataaata cacgatgccc catttagaaa aataccactg tcggcataat tgcaaccaaa
     5821 cccggcaacg ttgggatcgt taatcacacc caaccatcca catgtattac gtgtcatgta
     5881 tattttacca tcgggtccaa gttgcaaacc accaatatac gaaccaccaa gtggaatagt
     5941 tggtatgtcg aggcgcgatg cattcatagt tacggcattg cccgaagtaa gatcgaactg
     6001 aaataattta aatggaattt cggaagtagc gtaaagcaca ttgccactag gcgaaaactc
     6061 aacgctgtat gcaccttcgc tgctattagt tgaattaagg gaaataggat tgctcaacac
     6121 ccctgtagaa ggatcgaaat cgaaaacata aaacgtatcg ggcgcactca ctgccattgc
     6181 cactttaaaa ccatcgggcg aaaactttaa gttacctaga aaatccaacc ccgatccatt
     6241 aaacaccgga cctacgcttg taattacagg caccaaattc agccctgttg aggtaagaag
     6301 gtaagcaaca aattcgctgc ccaaactctg atgcgataga atccagatat cgttaccgtt
     6361 gctatgcgta tgtgctgtaa cacgctctgt agttggggta tacagtattt gattttttaa
     6421 tatcacactg cccaatccac catttgtgct aatatcaact atattgtatt gaaacccggc
     6481 agtgctgtta aactcgcccg aagtaaaaat ataatagttt gtattcacac ccggctgttt
     6541 tactatgcat gcagattgtg tagatgaact tcctccgccc aatcctgtgc cattggccat
     6601 aagtacatga tttttattcc aaacacttac accatcggta taaaaaagaa tagcaccatt
     6661 atcgtccgaa atactgctgc atccttcggc agtactcata gcactattat ttattgccac
     6721 cggtgcgcca ccattgaaat ccaaaccggc ttgaatgcca aagtaccatg tgttggcata
     6781 tttttgggcc tgtatgtttc cgctcatcat tgctataagc atcgtaaata agtaaaagtg
     6841 ttttttcatt ttgtatgtgt ttttagtttt agaatttaca tgcaaatatt caattttttt
     6901 tatggaatac aaatcgaata aagttattgg tttttcgagc atctcttagg caacaaaata
     6961 aatcaatgct tttcttatat ttgtactatt aattattgat tcttattgat agaagattta
     7021 tttggaatca atacttattt caaaaaatat aaaatggaaa taaccaagga ccttaaactg
     7081 gcggtactca tagatgccga taatgtgcct tatgcacaca taaaagaaat gctcgaagag
     7141 attgctaaat acggcactcc aacttttaaa cgcatatatg ccgactggac catgccaaca
     7201 gtaagcggat ggaaaagtgt actgctagaa agtgccatca cccctgtaca acaatatgga
     7261 tatacgaagg ggaaaaattc tactgatagt gccatgataa ttgatgctat ggatatttta
     7321 tattcggcaa aggttgatgg tttttgcatt gtttcgagcg atagtgattt tacacgcctt
     7381 gcaatacgct tgcgagaggc tggcatgaaa gtgataggaa taggagaaaa gaaaacacca
     7441 acatcattta tttcggcttg cgaaaaattt atttacatcg aaatattgaa accccaaatt
     7501 atagaagaga aaaaagtatc gaacggaaaa gttacaaagc aattaataca aaaagcacct
     7561 tcgctcaata aagttgatca tgcattgata aaactaattt ccgattccat taatgatttg
     7621 gccgatgaaa atggttgggc ttttctgggc gacctaggga atttattatt aaaaaaacaa
     7681 cccgacttcg atcctcgaaa ttatggtttt acaaaaatgt tatccctcat taaaagcatc
     7741 cctaagtttt tggtagatgc acgcgatgcc ggaaaaaata atatcaaaca catttttata
     7801 aaaataaaat aatgaaaagc atttctgcca aattatgctg cgcatgcttg ttcctttgca
     7861 tacatgcaaa tatttatagt aaaaatattt ccattctttt tatcggcaac agttatactt
     7921 atgtaaataa tttgcccgaa atggtgcgcc aaatgggact taccatggga gatactattt
     7981 ttgtagacag cagtactccc ggtggataca cttatcaatt gcacacaact gatgccaata
     8041 caattgcaaa aataaaacag caacagtggg attatgttgt gttgcaggaa caaagtcaac
     8101 taccctcctt atcgcctgcg tttgtaaatg tgaactccgt accctatgcc ctcatgcttg
     8161 atagtatgat aaaggcaaac aatgcttgta caaaaactat tttttatatg acatggggac
     8221 gcaagtatgg cgatggttct aattgtgctt cgtatccacc tgtttgtact tatgccggta
     8281 tgcaacaaag gcttaaggag tcgtatcttc tttttagcga tacctgcaag gcagtagcag
     8341 cacctgttgg catagctttt aaaaattata ttgccatcga tacacttctc gatttgtact
     8401 cacccgattt cagtcatcct tcgttggcgg gaagtttttt agccgcagct acaatttatt
     8461 caactatcac acacaatcaa attacaaatc aaaattattc tgccggcctt actgtaacac
     8521 aagcagcagc actacaaaat acgtcatggc aaactattat cgatagcagc gcactttgga
     8581 acctgggggt gaacacgcct tttgccaact tcacatacaa cactttgccc aatttgacag
     8641 tacaatttat ttgccccggc aatgtaaact atacccactt ttgggatttt ggtgatacta
     8701 cattttcaac cctggcagag cctgtacatc agtatttgta ttcatcttat ttcggagtaa
     8761 agcatattgt ttatgacagt tgcacctacg attcggctca ggttattacc aatataacac
     8821 cacaaaacat ttcaaatata actgttgatg aaagtaattt tgctttttac aatagtgccg
     8881 atgaaactat tgccatgagt ttatggaagc tcagttcaca caatctaact ataagcctat
     8941 acgatgtgtc ggggaaatta attttgaaaa ataattatac gccaacaaaa ccattaacat
     9001 atttaaatgt aagaaaaata agcaatggca tgtatatttt aaatgcagtg gatgacgtaa
     9061 ccggaaaata tttttcgcaa aaaataatta aaaattgatt ttaaaaactt gctgtgaatg
     9121 agggagttcg ataaaaaatg tttttaatta ttagaactac aatatctttt aatg
//
LOCUS       DAORQA010000060         9004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2045_length_9004_cov_8.510560, whole genome shotgun sequence.
ACCESSION   DAORQA010000060 DAORQA010000000
VERSION     DAORQA010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9004
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2045_length_9004_cov_8.510560"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /locus_tag="PK736_05030"
     CDS             complement(<1..94)
                     /locus_tag="PK736_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRC32787.1"
                     /translation="MFLEQSKQHIHKRGWIEVVCGSMFSGKTEEL"
     gene            196..1032
                     /locus_tag="PK736_05035"
     CDS             196..1032
                     /locus_tag="PK736_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32788.1"
                     /translation="MSRHLIINIAKSLEAVNDHFNTVNNHDGVVPQIEVDILKEHVRA
                     MYEHIITFQKQNQQSAMIHSRQREFINEPATPKVIPVQEMVGAQVAAPAPVPARPEIQ
                     KPEVILPRKPEPVIVQEKVEVAIPEKIVEAPIIEKAMPAISTAPVQEHVVSKPNKTKQ
                     VASLFDNAGVLAHQFDEQKSVADKIASDPQSSRVADAMHQSKIIDLKKAIGINEKFLF
                     INELFEGSMQAYTETIEKLNNSSDITQAKQLLEPLHMKYNWNTSIVAYQKLMDLVERK
                     VS"
     gene            1391..1954
                     /locus_tag="PK736_05040"
     CDS             1391..1954
                     /locus_tag="PK736_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRC32789.1"
                     /translation="MHRHFMLHKPDGYMSQFVCELKKKKVLGDFFDFPPGTMSIGRLD
                     EDSEGLLLLTTDGMMSENIRSKKVEKEYYVLIDGIITADALEKLCNGVEIGIHNEKYI
                     TLPCKAFVLDNDPDIAPRKKRIRDNRHGPVQWISITISEGKYRQVRKMTAAVGFPTLR
                     LVRVRIANMHLNNLKAGEVIEVEKFDY"
     gene            complement(2095..3963)
                     /locus_tag="PK736_05045"
     CDS             complement(2095..3963)
                     /locus_tag="PK736_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC32790.1"
                     /translation="MKVKATKEVKPAKSQSADLNLPWYLQWRNQVIFLIVLVFAVFGI
                     SVLNEYALDDFILIVKNSYTQQGFAGIKNILTKDTFAGMSEGNVMVLAGGRYRPLSVI
                     SFAMEHQFFNGNPHISHFINIASYAAVVVALLWILRNIIFRNHPLVAFFVAAIFAIHP
                     LHTEDVANIKGRDDIFCLLFFFLSIIWLYKYINENKFTQLAASTIFFFLSLLSKEFGV
                     ALFFLIPITFLIFTNSARTKSISTYLPHFISMLAFVAMRYSATVDNGGTPSNDVFNNP
                     WFSLSFTDKYGTIFSTWLVYLRNMFVPLNMSYDYNFAHVDIVSLTNFYALISIVIHLT
                     MAVYAAINIKKQPLIAYGIIFYFISFGLVSNLLFNIGATMADRFMFIPSVGFLIALAG
                     IYILVKEKYAQQIKAIQISAIGFAIICITAGSVYSAIRCTAWKNNNTLFKNDVHHVPK
                     SVKANLNAGIAYLDTLDDTSTKHSLENAKYYLQKGIAMKPDFLDGYLNMGVIYNWQQN
                     NDSALWWWLKAREVKPGNSTVNQYLEMLANPIIQKGLDAKKDFAASISYFEQALKIDN
                     RNAETLYNIGGAYYSLNDFANAEKYWQQCLAINPLHQQAKAGLNAATNQLQLLKIK"
     gene            complement(4004..4876)
                     /locus_tag="PK736_05050"
     CDS             complement(4004..4876)
                     /locus_tag="PK736_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRC32791.1"
                     /translation="MVKSMTGFGRAVGHINNNQVTVEIKSLNSKFLEINLRLAQSLRD
                     REMDIRQHIMKTLERGKIDISISVDETDMQSPTNFNRKVIDAYITELQSIATQNNIVI
                     HDMLSIITALPNAVTLQKNETDDEAFAQLMKVVNDCIANFDQFRKKEGEALQQDLLNN
                     LQSIENHLEQINSIAPHRIENIKQRILLQLEAASENITVDKNRFEQELIYFLERLDIN
                     EEKVRLISHLNYFTDHVKGKEVGGKKLGFIIQEMGREINTMGAKANDAAIQVHIINMK
                     DDLEKMKEQVLNIV"
     gene            complement(5105..6337)
                     /gene="serA"
                     /locus_tag="PK736_05055"
     CDS             complement(5105..6337)
                     /gene="serA"
                     /locus_tag="PK736_05055"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRC32792.1"
                     /translation="MTTGTTFPKEKIKILLAENINASAVNMLREHGYKNIDTHKAALS
                     EQELLKLAPQYHVVGIRSKTKLTANFFDKADKLLCVGAFCIGTNQVHTESAMAAGVAL
                     FNSPYSNTRSVAELTIAECVMLLRKAFEKSQQMHQHIWSKESKGCFEVRGKTIGIIGY
                     GHIGSQLSVLAEALGMRVVFYDVVNKMALGNSQQCKTLEQLLTVSDIVTIHVPSQQGT
                     KNLINHDCIRRMKKNAVLINNSRGDVVDIASVANALRANKLAGYAADVFPTEPASNTE
                     PFTSELCGMSNVILTPHIGGSTEEAQVNIGTDVASKIIDFIETGSTAGSLTIPELSLP
                     IVHGTHRFLHIHNNVPGVLSEINGLLSSQKVNILGQYLKTNSTIGYVVLDIDKKTSQN
                     VLRELKSVKNTIKVRSLF"
     gene            complement(6342..8105)
                     /gene="sppA"
                     /locus_tag="PK736_05060"
     CDS             complement(6342..8105)
                     /gene="sppA"
                     /locus_tag="PK736_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRC32793.1"
                     /translation="MRQFFKFFFASMLGFIVGSVVLFFLLILMIAGVVSTMSGPKTMA
                     IHGPHVLKINLQQPLTERTPNNPFRNFRAGEIKSQMQPGVYDIIEDLDKAANDKNIKG
                     IYLNITDIDGGISSVEEIRNAILKFKKSKKFVIAYSEYYSNKGYYLASAADKVYLNPE
                     GELDFRGYATQLMFLKGMLEKVGVEPQIIRHGKFKSAVEPLTEEKMSDANREQMKALI
                     DDLWKHTLAGIGKSRNKTYDELELMADSLLIESPADAVKYGLIDGTKYEDEIMKMLTE
                     KSETKDDKVDFVSLEKYNNVPSGKKFHAQKIALIFATGSIGSGKGDDESIGSETTADA
                     IRKAREDKNVKAIVLRVNSPGGSALASDVIWREVTLAKKEKPVVVSMGDLAASGGYYI
                     SCAADKIYAQPNTITGSIGVFGVIFNAQKMLNEKLGLTFDTYKTGEYGDMGLPTRALT
                     PGERRKIQNSVENVYDQFTKKVGDGRGIAQANVDSIGQGRVWSGEDALQIKLVDELGG
                     INDAIAAAAKLAKITEYRVSQLPVQKEPFVELLEGGADDVKQSMINSELGANAKYYKS
                     LKDIFKMQGIQVRLPYDITIQ"
     gene            complement(8155..8676)
                     /locus_tag="PK736_05065"
     CDS             complement(8155..8676)
                     /locus_tag="PK736_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRC32794.1"
                     /translation="MALIKSVNGITPKFGNNCYLAENATVVGQVTMGDNCSVWFNTVV
                     RGDVNKITLGNNVNIQDGAVVHCTYEKAETVIGNNVSIGHNAIVHGCTVMDNALIGMG
                     AIVMDNAVVHLNSIVAAGAVVLENTIVEEGSIYAGVPAKKVKQISPELFKEMNMRIAN
                     NYLMYKEWFASND"
     gene            8802..>9004
                     /locus_tag="PK736_05070"
     CDS             8802..>9004
                     /locus_tag="PK736_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32795.1"
                     /translation="MKSVFGITGIIILIAIQSCKKTEEVSPIPSIELEYIQVNNNGFI
                     DTSASVKIFYRDGDGDLGSLNDNT"
BASE COUNT         2692 a         1772 c         1659 g         2881 t
ORIGIN      
        1 taagttcttc tgttttgccc gaaaacattg acccgcaaac cacctctatc catccgcgtt
       61 tatgtatgtg ttgtttcgat tgttctaaaa acatgtaact gttggcaact attaaaaatt
      121 ttgagcaaag atggtactat aaattctact tttgaatcat caaacaaata aaccaaaata
      181 aattttatat ctaaaatgag cagacactta ataatcaata ttgcgaaatc gcttgaggct
      241 gtaaatgacc acttcaatac tgtaaacaat catgatggag tggttcctca aatagaagta
      301 gacatattga aagagcatgt gcgtgcgatg tacgaacata ttattacctt tcaaaagcag
      361 aatcaacaaa gtgcgatgat tcactcacgt cagcgcgaat ttattaacga accagcaaca
      421 ccaaaagtta ttcctgttca agaaatggtt ggcgcacaag tagcggcacc tgcgccagtg
      481 cctgcgcgac cagaaattca aaagcctgaa gtgatattgc cacgcaaacc ggaaccagtt
      541 attgttcagg aaaaagtgga agtagccata cccgaaaaaa tagtagaagc accaattata
      601 gaaaaggcaa tgcctgcaat aagcactgcg cctgtgcagg agcatgttgt gtcaaagccg
      661 aataaaacaa aacaggtggc cagtttattt gacaatgccg gagtgcttgc acatcagttt
      721 gacgagcaaa aaagtgtagc cgataaaatt gcctccgacc cacaatcgag ccgtgttgcc
      781 gatgcaatgc atcaatcgaa aataattgac ttgaaaaaag ctattggcat aaacgaaaaa
      841 ttccttttta taaatgaatt gttcgaaggc agtatgcaag catataccga aaccatagaa
      901 aaactaaaca atagttcaga tattactcag gcaaaacaat tgctcgagcc tttgcacatg
      961 aagtacaact ggaatacttc tatagtagcc tatcaaaaat taatggattt agtagagcgc
     1021 aaagtttcat aatttttatt ttcaaaaacg atacggcttc gataggattt tttgccacgc
     1081 ttgccccgtt gttcggggaa tgggacgaat gaatgccgaa taattttttt tgtatatgtt
     1141 aggtgtctat ggtttggcgt ctccgcaaac cttctacaaa gctcgaaaat tgtccggggt
     1201 tggcgtcctc gccaacctct catataacac gaaaatttat ttcacaatta ttttttctta
     1261 tagttgaaaa tctaaaaaaa caatttcata ttgcgaaaag tcaacacttt aaaattataa
     1321 catttttgaa atttttttat taaaaaaacc gacttttgat tttagcaact aataaaaaaa
     1381 tcaatttata atgcaccgcc attttatgtt gcataagccc gatgggtata tgagtcagtt
     1441 tgtgtgtgag ttgaaaaaga aaaaagttct tggcgatttt ttcgactttc cccccggtac
     1501 aatgtccatt ggtaggctcg atgaagacag cgaaggactt ctgctgctta ctaccgatgg
     1561 catgatgagt gaaaatattc gcagcaaaaa agtagagaaa gaatactacg ttttgataga
     1621 tggaataata accgctgatg cccttgaaaa attatgcaac ggtgttgaaa taggaattca
     1681 caacgaaaaa tatattaccc ttccatgcaa ggcatttgtg cttgataacg accctgatat
     1741 cgctccacgc aaaaaacgta ttcgcgacaa caggcatggg cctgtgcaat ggatatccat
     1801 tacaattagt gaaggcaaat accgacaggt gcgcaaaatg actgccgctg ttgggtttcc
     1861 tacattgcga ttggttcggg tgcgcatagc aaatatgcat ttgaataatt taaaagccgg
     1921 tgaagtaata gaagtagaaa aattcgatta ctgaaatcta acaaatcaga aaaaatattt
     1981 ttttgcactg tatgcacaaa aaacacgaaa gggttttgtt ttcaaatgcc gaatattttt
     2041 tcgcctctga ttgcactaaa ggcaaaaaag ggttctggac cgaatggaaa acctttattt
     2101 tatcttgagt aattgtaatt gatttgtggc tgcatttaac ccggcttttg cttgttgatg
     2161 aagtgggttt attgccaaac attgctgcca gtatttttca gcattcgcaa aatcgtttaa
     2221 cgaataataa gcaccaccaa tgttgtacaa agtttcggca ttgcgattat caattttgag
     2281 cgcttgctcg aaataagaaa tgcttgccgc gaaatccttt ttggcatcaa gtcctttttg
     2341 aattattgga ttggcaagca tttcgaggta ttgatttact gttgagttgc cgggttttac
     2401 ttcgcgcgct tttagccacc accacaatgc tgagtcattg ttttgctgcc aattgtaaat
     2461 tactcccatg tttaaatatc catccaaaaa atcaggtttc atggctatac ctttttgcaa
     2521 ataatatttg gcattttcta aactgtgttt tgtactggtg tcgtcaagtg tatcaagata
     2581 tgctatgccg gcattgagat ttgcctttac actctttggc acatgatgca catcgttttt
     2641 aaaaagtgtg ttgttgtttt tccatgctgt gcaacgaatg gctgagtaca cgctgccggc
     2701 agtaatgcaa attatggcaa aacctatggc tgaaatttgt atagctttta tttgctgcgc
     2761 gtatttttct tttaccaata tatatattcc cgccaaagca attaaaaaac ctaccgatgg
     2821 tataaacata aaacgatcag ccatagttgc gccaatattg aaaagtaaat tcgataccaa
     2881 accgaacgag ataaaataga atattatgcc ataggctatg agtggttgct ttttgatatt
     2941 tatggcagcg tatacagcca tagttaaatg tattactatc gaaattaggg cataaaaatt
     3001 tgtgagtgat acaatgtcaa catgagcaaa attgtaatcg taactcatat tgagcggaac
     3061 aaacatattg cgcaaataaa ctaaccaggt cgaaaatata gttccgtatt tgtcggtaaa
     3121 ggataaggaa aaccatgggt tattaaacac atcattactt ggtgtaccgc cattatcaac
     3181 cgttgcactg tatcgcatgg cgacaaaagc aagcatggaa ataaaatgcg gcaggtaggt
     3241 tgatattgat tttgttcgtg cgctatttgt aaaaattaaa aatgtaattg gaattaaaaa
     3301 aaacaaagcc acaccaaatt ctttgctgag taacgataga aaaaagaata ttgttgatgc
     3361 tgcaagttgc gtaaatttat tttcgtttat atacttataa agccatatta tagaaaggaa
     3421 gaaaaataat aagcaaaaaa tatcatcacg gccttttatg tttgccacat cttcggtatg
     3481 aagcggatga atagcaaaaa tagctgccac aaaaaatgca acaagtggat ggttgcgaaa
     3541 aataatattg cgaagaatcc ataacaatgc aacaacgaca gctgcataac ttgcaatatt
     3601 gataaagtgc gaaatgtgtg gattgccgtt aaaaaattga tgttccattg caaacgaaat
     3661 aacagatagc gggcgatagc ggcctcctgc aagtaccatt acattgcctt cgctcatgcc
     3721 ggcaaaagta tctttggtaa gaatattttt tatgcctgca aaaccttgtt gggtgtaact
     3781 attttttact attaaaataa aatcgtcaag ggcatattcg ttcaaaacag aaataccaaa
     3841 cacagcaaaa actaaaacga ttagaaatat aacttgattg cgccattgta aataccaggg
     3901 caaattcaaa tcagcacttt gcgattttgc gggtttaact tcttttgttg cttttacttt
     3961 cataaggcaa aataacagtt atcaaaaaga aaatatattt gagttaaaca atatttaaaa
     4021 cctgttcttt cattttttct aaatcatctt tcatattgat gatgtgaacc tgaatggcgg
     4081 catcgttggc tttggcaccc atcgtattta tttcgcggcc catttcctga ataataaatc
     4141 caagcttttt accaccaact tcctttccct ttacatggtc ggtaaaataa ttcaagtggc
     4201 ttatcagtct tactttttct tcgttaatgt ccagtctttc cagaaagtaa atcaattcct
     4261 gttcgaagcg atttttatca acagtaatat tttcggaggc agcttctaac tgtagtaata
     4321 tacgttgctt tatattttcg atgcggtgtg gagcgatgct attaatttgc tccaaatgat
     4381 tttcgatact ttgcaaattg ttgagcaaat cttgctgcag tgcttcgcct tccttcttcc
     4441 tgaattggtc gaagttggct atacaatcat tcaccacctt cattaattgt gcgaatgctt
     4501 cgtcatcggt ttcgttcttt tgaagtgtca ccgcatttgg cagtgcagtt ataatggaaa
     4561 gcatatcgtg tatcacaata ttgttttgtg tggctatgct ttgcaattcg gtaatgtagg
     4621 catcaattac tttgcggtta aaattggtgg gcgattgcat atctgtttca tccaccgaaa
     4681 tactgatatc gattttgcca cgctccagtg ttttcataat gtgctgccga atgtccattt
     4741 cgcggtcgcg cagcgattgt gccaggcgca aattgatttc gagaaactta ctgttaagcg
     4801 attttatttc taccgttacc tggttgttgt taatatgccc caccgcacgg ccaaagcctg
     4861 tcattgattt taccataata aaatatttac acaaatgtaa acattgaatt ggattattga
     4921 atagaaggat gctaatgaga atttgtacgc cctttcaatt aaacctaaat attaatttga
     4981 aaacgcccaa agaagtgatt taaagcacag caatatgaaa cctcgatagc gcaaaaaggt
     5041 ttgttgagta caaaaaatta tggtatcggt ttatgataga aatttgtatc gcaaagtaaa
     5101 ctacctaaaa caaactccta accttaatag tattcttaac cgatttaagt tcgcggagta
     5161 cgttttgcga tgttttttta tcgatgtcta aaaccacata gcctatagtg ctatttgttt
     5221 tgaggtattg gccaagtatg ttgacttttt gtgaggacag caatccatta atttcggaaa
     5281 gcacgccagg tacattattg tgaatatgca gaaagcgatg tgtgccatgc acaattggta
     5341 aacttaattc gggaatggta agcgagcctg ccgtgcttcc tgtttcaata aagtctataa
     5401 tttttgatgc tacatcggtg ccgatgttta cttgtgcttc ttcggtgctg cctcctatgt
     5461 gtggcgtaag tattacattg ctcattccgc aaagctcgct ggtaaagggt tcggtgttgc
     5521 tggcaggttc ggtagggaaa acatcggcag catatcctgc aagtttattt gcgcgcaatg
     5581 catttgccac agaggcaata tctaccacat cgccccgcga attatttatt aatacagcat
     5641 tctttttcat cctacgtata cagtcgtggt tgataagatt ttttgtgcct tgttgcgatg
     5701 gtacatgtat ggtaactata tcactcacgg ttaataattg ctccaatgtt ttgcattgtt
     5761 gcgaattgcc taaggccatt ttatttacca catcgtaaaa cactacgcgc attcccaatg
     5821 cttcggcaag caccgacagt tgcgagccga tatgcccgta accaataatg ccaatggttt
     5881 tgccacgtac ttcaaagcaa cctttacttt ctttgctcca aatatgttga tgcatttgtt
     5941 ggcttttttc aaatgctttg cgcagcaaca tcacacactc ggcaatagtt aactcggcaa
     6001 cagaccgtgt attggaatag ggcgaattga ataatgccac acccgcagcc attgccgact
     6061 cggtgtgcac ctgattggta ccaatacaaa aagcgccaac gcaaagtaat ttatctgcct
     6121 tatcaaaaaa atttgctgtc agttttgttt tactccgaat tcccactaca tgatactgcg
     6181 gtgcaagttt caataactct tgctcgctaa gtgctgcctt gtgtgtatca atatttttgt
     6241 aaccatgttc gcgcaacata ttcactgccg aggcatttat attttcggcc agcagtattt
     6301 ttattttttc ttttgggaat gttgttcctg tggtcatata tttattgaat agttatatcg
     6361 taaggtaacc gtacctgtat accctgcatt ttgaaaatgt cttttagcga tttataatat
     6421 tttgcattag cgccaagctc actgttaatc atactctgct ttacatcatc ggcacctcct
     6481 tcgagcaatt ctacaaaggg ttctttttgc accggcaatt gcgatacacg gtattctgta
     6541 attttagcaa gcttggcagc agccgcaatg gcatcattta ttccgccaag ttcatctact
     6601 aattttattt gcaaagcatc ttcgccactc cacacacggc cttgtcctat actatcgaca
     6661 ttggcttgtg caataccgcg tccgtcacct acttttttag taaactgatc gtaaacattt
     6721 tccacagagt tttgaatctt acgtctttcg ccaggtgtga gtgcacgtgt aggcaaaccc
     6781 atatcgccat actctccggt tttgtaagta tcgaaagtga ggccgagttt ttcgttcagc
     6841 attttttgtg cattgaaaat aacaccaaac acaccaatcg atccggtgat ggtatttggt
     6901 tgcgcataaa ttttatcggc agcgcaacta atataatatc cgcccgatgc tgcaaggtcg
     6961 cccatcgata ccactacagg tttttctttt tttgctaaag taacttcgcg ccatattacg
     7021 tcacttgcaa gtgcactgcc accgggcgag tttacgcgca acactatagc ttttacattt
     7081 ttatcttcgc gtgctttgcg tattgcatcg gctgtggttt cgctgcctat cgattcgtca
     7141 tcgcccttgc cgcttcctat acttccggtg gcaaatatca atgcaatttt ttgcgcatgg
     7201 aatttttttc cagaaggcac attattatat ttttcgagcg aaacaaaatc tactttatcg
     7261 tcttttgttt cacttttttc agtaagcatt ttcattattt catcttcgta ttttgttccg
     7321 tctatcaaac catatttcac cgcatcggca ggcgattcta tcaacaggct atcggccatc
     7381 agttcaagtt catcgtatgt tttattgcgg cttttaccaa tgccggccaa agtatgtttc
     7441 cacaagtcat ctatcaatgc cttcatttgt tcgcgattgg catcgctcat tttttcttcg
     7501 gtgagtggtt ctactgcgct tttaaatttg ccatgccgaa taatttgtgg ctccacacca
     7561 accttttcga gcataccttt tagaaacatc agctgcgttg catagcctct aaaatcaagt
     7621 tctccttcgg gatttaaata aactttatcg gctgccgaag ccagataata tcctttgttg
     7681 gaataatatt cgctataggc aattacaaat tttttcgact ttttaaattt caatatcgca
     7741 ttgcgaattt cttctaccga tgatatacca ccatcaatat cagtaatatt taagtagatg
     7801 ccttttatat ttttatcgtt ggcagctttg tccaaatctt caataatatc atacactccc
     7861 ggctgcattt gcgattttat ttcgcccgct ctaaagttgc gaaaaggatt gttgggcgtt
     7921 cgctcggtga gcggttgctg caagtttatt tttaaaacat gcggcccatg tatggccatt
     7981 gtttttggcc ccgacatagt tgataccaca ccggctatca tcaatatcaa taaaaagaat
     8041 agcaccaccg atcctacaat gaaccctagc atggatgcga aaaagaattt aaaaaattgt
     8101 ctcattttgt atttgtttta agtttagata aaatattttt cttttgttac attattagtc
     8161 attgcttgca aaccactctt tatacattaa gtaattattg gcaatgcgca tattcatttc
     8221 cttaaataat tcgggcgaaa tctgtttcac tttttttgcc ggcactcctg cataaatgct
     8281 tccttcttct acaatggtgt tttctaatac aacagcaccc gcagctacta tcgaattaag
     8341 atgtaccacc gcattatcca tcactattgc acccatgcca atcaatgcat tatccattac
     8401 cgtacagcca tgcactatgg cattatgccc aatcgaaaca ttgttgccaa ttacagtttc
     8461 ggccttttcg taagtgcaat gtacaacagc gccatcctga atatttacat tattacctaa
     8521 ggtaatttta tttacatcgc cacgaacaac agtattaaac cacacactac aattatcgcc
     8581 catggttact tgccctacaa cagtagcatt ttcggccaga tagcaattgt tgccaaactt
     8641 aggagtaatg ccatttacag attttattag tgccatttta ttttttaaaa aacaggagaa
     8701 atatattttt tttgtaaaat tacattcagg caacaaacgt acgtgtataa tcgttaattt
     8761 tgaaacttaa actaaatacc atggattttt gttttaaaaa tatgaaaagt gttttcggca
     8821 taacgggaat tattattttg attgcaattc agtcttgcaa aaagacagaa gaagtgagcc
     8881 cgattccttc tatcgagttg gagtatatac aagtaaataa taatggcttt attgatactt
     8941 cggcatcggt aaaaatattt tatcgcgatg gcgatggcga cttgggtagc ttgaatgata
     9001 atac
//
LOCUS       DAORQA010000061         8973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2056_length_8973_cov_18.988787, whole genome shotgun sequence.
ACCESSION   DAORQA010000061 DAORQA010000000
VERSION     DAORQA010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8973
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2056_length_8973_cov_18.988787"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1998
                     /locus_tag="PK736_05075"
     CDS             <1..1998
                     /locus_tag="PK736_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32796.1"
                     /translation="VDTKVPEEEKSTLFSFKFKVDLFTKVEGLDSIANFRLNKPVAKL
                     KYDDSYDDFNYDEAYSKQRKAELDVVKNDMETLKALRDKEAADLLAKQMADSVANYKV
                     DLEAKNKLAAQQKAEADSLAKLNTEKSKAEIEAAAAAAAAKAKEDEEARIKQLEQDKL
                     NAIAEAKAKKDSLEKFKVDEQARLESEAKARKDSLAQAEAEAKAKAAADEEARLKSIA
                     EEKAKKEAEALAKKESEQKEKERIAAEALAKKDADAKAKADADSTAAFNKAEAKRKAE
                     EEKVKAIADAKAKADADKAERERIAAEELAKKEAAAKAKAEADSIAAFNTAETKRKVE
                     EEKAKAIADAKAKADAEKAERERIAAEAKAKQEEIAKADSLAKFNVAEAKRKADEDKI
                     KKDAEAKAAMEAERKRIEEEKKAKIEADAMAKVTADSLKAFNIAETKRLAEEARLKKA
                     ADEKARVQAIEDAKAKAIADAKAKAEEEERLKAEEAAKIQAAQEAAELARIEKEEAAR
                     AESLRIFNEKKAAQAKIDAEKNKNVDPKILAERAAKAKADSLALIENEINSINDKENK
                     NKALTAAEVKRMQELQTLRDAMTSASALKKEYAEGITEEKIDEAKLTTYKTIVKRKSS
                     QEVYKKVVYNWGGVFYFKNDQSISELSYNLLINNAKKELKK"
     gene            2083..3288
                     /locus_tag="PK736_05080"
     CDS             2083..3288
                     /locus_tag="PK736_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC32797.1"
                     /translation="MENQQKNVTRILILMAIMQFTNAMDFMIIMPLSVHLIPKFNINS
                     SQFSMLVAIYAFSAFASSVSASFYADKFDRKKVLLAAYVGLVAGTLFCGLANSFSTML
                     LARCFTGIFGGLIGAQTLAIAGDIVPFERRGKAVGILMSGFSLASVAGVPVGIYLANT
                     FSWHIPFITIASIGFLLIPFCFSIIPSVTAHIKSNDNGQNFEVYKSIVADKKQQGALL
                     MMFTLTVSHFSIIPFIAPYMESNVGFTKNQLALIYFVGGAVTLVSSPLIGKLADKYGK
                     FKTFFVLILFSFIPVILITNMPAIPVYQALIVTAFFFIFGSGRMIPSQAIATSVVGNK
                     LRGSFMNINSSVMQLATGIASLIAGLIVTRSNSGTIFNYQWVGLFSVAMCIICIVVAF
                     RLQKQINLK"
     gene            3313..4332
                     /gene="mltG"
                     /locus_tag="PK736_05085"
     CDS             3313..4332
                     /gene="mltG"
                     /locus_tag="PK736_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRC32798.1"
                     /translation="MMKYFVLIILLAVVAVGFFAYNYLYKRNTGFDEKSKLIYIHTGA
                     NFSDVVNKLKSENIIKDVTAFEWLSKKKKYTSKVRPGCYRITKGMTNEAIVNLLRSGI
                     QQPVEVKFNNLRIPSQLAGAIAGQIEADSLSLYKMLVDENVAKSYGFNVNSFIAMYVP
                     NTYEFYWNTSAEGFVKRMAKEYKSFWNISRKAKAKKWNLTESEITTLASIVQEETNMA
                     TDKPIVAGVYLNRLKKNIPLEADPTLKFALQDFTIKRILNVDKEVISPYNTYKNQGLP
                     PGPICIPDVSTIDAVLNATEHDYIFFCAKEDFSGYSNFAVTNAEHEINAKKYQQALNK
                     MNIKR"
     gene            4334..6058
                     /locus_tag="PK736_05090"
     CDS             4334..6058
                     /locus_tag="PK736_05090"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HRC32799.1"
                     /translation="MHYKIFSQSPHTHFINIECTIENIDKDELILKLPVWRPGRYERV
                     NFAKNIGSFNIVDSHGHDVPFYKKESSIWVVDTKTASSLKIIYTYFANQPDAGACFCN
                     NEILYINPIHCCVYLDERMPLQHTMDFITNDDWQTATSLSKDTNNFYVANDFHELVDS
                     PIIAAKQMQHHSYSTHNTLFNIWIQGEFENNIEKLIADFKLFSDLQLKVMRDIPVSEY
                     HFLIIITPHKFYHGVEHLKSTVLAIGNAEELLKDEWYNELIGVASHELFHVWNVKTIR
                     PAEMLPYKYDTENYAATGYVYEGITTYYGDLFLARCEYLNHHQYLEEINTRLNKHYHN
                     EGRLNYSVMQSSFDTWLDGYVPGVPGRKTSIYDEGSLIALILDLHIIHYSNATKSLDD
                     VMQVLYNDFGKNNLGYKHHDIQRVCENIAGLSFANIFNNLIEKNISYETALDQALNFV
                     GCKITLVQSKNSCEKNYGMKLIDEHKNERWRVTAVQKNSPADIGGIAVNDVLTNACYS
                     IICDNNINHIFDHDKCEVYFDSYIKQRYTVLHANGKTFFDMLKVESIETKTEQQTKLY
                     NYWLGERN"
     gene            complement(6120..>8973)
                     /locus_tag="PK736_05095"
     CDS             complement(6120..>8973)
                     /locus_tag="PK736_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32800.1"
                     /translation="KKIIFTILLGAWLLPGINFAQNRFREKFEEIKAGDNFYTIQKEL
                     NDYYAEHATGQGSGYKQYKRWEDYMAPRVYPSGKIMNIEAKVWEEYFAYEDNAKKGDP
                     NTTFTHSGYWSFVGPTTHVKGNSGYNGGVGRVNCIARHPTLASNIFVGTPSGGIWKST
                     TSGNAWTSLSDGIPRLGVSGIVVISTNTIYILTGDGDGSDTYSVGVLKTTNGGVTWKK
                     TGLSFTPTDYVKGYKLMVDPTNSSILYAVTTNGLYKTANAGTSWTLVKSGSFRDCEFK
                     PGTPATLYLVSTTTFYLSTDAGATWVTGAVGLPTSGSNRIAIGVSANNTAYVYLLYGK
                     SSGFMGLYRSFDSGLNFGLKSSTPNILGYDIAGGDFESQSSYDLAIAVDPSNVANVII
                     GGINTWRSTDFGATWTNSSYWVEDATPAQYTHADIHALEYFGTSLYCGSDGGIYRSLD
                     KGVIWADRSTGLGITQFYNLGLLQSNSAKILGGAQDNGSNQLNGATFTHFYGADGFEV
                     AYRESTPTTLFIESQNGGIRRSTNSGSTFTSIVPPGATGAGWNTPYIIDPANDARMYI
                     GYDKCYRSTNSGTAWTQIGGSLGTGVDLTACPSNSSRLYYVTSSKVWRSDNCTAAAPT
                     FTNVTGTLPVASASITSITVDNTNSLNAFVSFSGFSAANKVFETIDGGVTWTNISTGL
                     PNIPVNCILYHNGSADGIYVGTDVGVYYRDDALGTWIPFMNGLPNVIVRELEIHTSTN
                     QLVAATYGRGFWKSDTYSGCPSGYSLSTSNYQDQGYFYFEASSSISSSMHLYGGTGTD
                     CNYNAGSYIDLVDGFEITDGSLLHAYIDGCTGTSNPFPIQKLSGSYAGPMDDKVEDIL
                     QAQNQSFKIDDFELYPVPVHNTVNVSYTLNDDSPLKISVVGIDGKQMNVLMNQSDMKA
                     GVYRLNFDTSNLNNGIYFMRIETKAGMVTKRFSKVN"
BASE COUNT         3088 a         1710 c         1719 g         2456 t
ORIGIN      
        1 gtggatacaa aggtgcccga agaggaaaaa tctactctgt tctcatttaa atttaaggtt
       61 gatttattca ccaaagttga agggcttgat tccattgcaa atttcagact caataaacct
      121 gttgcgaaat tgaaatacga tgatagctat gatgacttta attacgatga agcatattcg
      181 aaacaacgca aggctgaatt ggatgttgta aaaaatgata tggaaacttt gaaggctttg
      241 cgtgataaag aagcagccga tttgcttgcc aaacaaatgg ctgattccgt tgcaaattat
      301 aaagttgatt tagaagcaaa aaacaaatta gcagcacaac aaaaagcaga agccgatagc
      361 cttgcgaaac tgaataccga aaaaagtaaa gccgaaatag aagctgccgc tgctgccgct
      421 gccgcaaaag ctaaagagga tgaagaagcg cgcataaaac aattggaaca agataagtta
      481 aatgctatag ccgaagcaaa ggccaaaaaa gattcgctcg aaaaatttaa agttgacgag
      541 caagcccgcc tcgaatcgga agctaaagcc cgcaaagatt ctctcgcaca agctgaggct
      601 gaagcaaaag caaaagcagc tgccgatgaa gaggcgcgac taaaaagtat agcagaagaa
      661 aaagcaaaaa aagaagccga ggctttagca aaaaaagaat ctgaacaaaa agaaaaagag
      721 cgtatagctg ctgaagcact tgcaaaaaaa gatgccgatg caaaagccaa agcagatgca
      781 gactcaacag cagcatttaa taaagccgaa gcaaaacgaa aagccgaaga agaaaaagtg
      841 aaagccattg ccgatgcaaa agcaaaagct gatgctgata aagcagaacg cgaaagaatt
      901 gctgccgaag aacttgctaa aaaagaagcc gcagccaaag ctaaagcaga agcagattct
      961 attgcagcat tcaacactgc agaaacaaaa cgaaaagtag aagaagaaaa agcaaaagcc
     1021 attgccgatg caaaagcgaa agcagatgcc gaaaaagcag aacgcgaaag aattgctgcc
     1081 gaagcaaaag caaaacagga ggaaattgcc aaggccgatt cgcttgcaaa atttaatgtt
     1141 gctgaagcaa aacgcaaagc tgatgaagac aaaataaaaa aagatgctga agccaaagct
     1201 gcaatggaag cagaacgtaa gcgcatagaa gaagagaaaa aagccaaaat agaagcagat
     1261 gcaatggcta aagtcactgc cgatagttta aaagcattta atattgccga aacaaaacgc
     1321 cttgctgaag aagcacggtt aaaaaaagct gccgatgaaa aagcacgggt acaggccata
     1381 gaagatgcaa aagcaaaagc cattgcggat gccaaagcaa aagccgaaga ggaagaaaga
     1441 ttaaaagccg aggaagcagc aaaaatacag gcagcacaag aagcagctga acttgcacgt
     1501 atagaaaaag aagaggcggc acgtgccgaa tcgttgcgca tatttaatga gaaaaaagca
     1561 gcacaggcaa aaatagatgc ggagaaaaat aaaaatgtcg acccaaaaat attagcagaa
     1621 cgtgcagcca aagccaaagc tgatagtctg gcactaatag aaaatgaaat aaattcaata
     1681 aacgataaag agaataaaaa caaagcactt accgctgccg aagtaaagcg tatgcaggag
     1741 ttgcaaactt tgcgtgatgc tatgacaagt gcctcggccc tgaaaaaaga atatgccgaa
     1801 ggaataactg aagaaaaaat tgacgaagca aaattgacta cttataaaac cattgtaaaa
     1861 agaaaaagca gtcaagaggt atacaagaaa gtggtgtata attggggagg tgttttctat
     1921 ttcaaaaacg atcaatctat ttctgaactg tcttataatt tgttaatcaa caatgcaaaa
     1981 aaagaattga agaaatagtt tttcgaaaaa aaaatttatc atcatacaaa gccggcaaca
     2041 tcaaaatttg ttgccggctt tttttattac atctttgccg caatggaaaa tcaacaaaaa
     2101 aatgttacac gtatattgat attaatggcc ataatgcaat tcaccaatgc catggatttt
     2161 atgataatta tgccgctgag tgtacatctc attccaaagt ttaacatcaa ctcatcgcaa
     2221 tttagtatgc ttgtggctat ttatgctttc agcgcatttg cttcaagtgt atcggcatca
     2281 ttctatgccg ataaattcga ccgcaaaaaa gttttgttag cagcctatgt tggtcttgtt
     2341 gcaggcacac ttttttgtgg attggcaaac agcttttcca ctatgctttt ggcgcggtgt
     2401 tttacaggaa tttttggtgg actcataggt gcacaaacgt tggcaattgc cggtgatatt
     2461 gttccttttg aaagacgcgg taaagcagta ggtatactta tgagtgggtt ttcgctcgca
     2521 tcggttgcgg gtgtaccggt aggcatttat cttgcaaata catttagctg gcacattcct
     2581 tttattacca ttgccagcat cggatttttg ctcataccat tttgctttag cattattcct
     2641 tcggttacag cgcatataaa atcgaacgat aacgggcaaa acttcgaagt gtataaaagt
     2701 attgtagccg ataaaaaaca gcaaggcgcc ttgcttatga tgtttaccct caccgtttct
     2761 catttcagca tcattccatt cattgcacct tatatggaat cgaatgttgg ctttacaaaa
     2821 aatcagctcg ctttaatata ttttgtgggt ggcgcagtca cgttagtttc ctctccatta
     2881 ataggcaaac ttgccgacaa gtatggaaag tttaaaacat tttttgtttt gattttattc
     2941 tcgtttattc ctgtaatact cattaccaat atgccggcca tacctgttta tcaagccttg
     3001 attgttactg cgttcttttt tatttttggc tcggggcgaa tgataccatc acaagcaata
     3061 gcaacatcgg tagttggaaa taaattgcgt ggaagtttca tgaatatcaa ttcatcggta
     3121 atgcagcttg ctaccggcat tgcatcgctt attgccggat taatagtaac gcgaagcaac
     3181 agcggtacaa tatttaatta tcaatgggtg ggattatttt cggttgcgat gtgtataata
     3241 tgcatcgttg ttgcatttag attacaaaaa caaataaatt taaaataaaa ttttcttttc
     3301 ataatgaaaa aaatgatgaa atattttgtg ctgattattc tattggctgt ggttgctgta
     3361 ggattctttg catacaacta tctatataaa cgaaacacag gtttcgatga aaaatcgaaa
     3421 ctgatataca ttcatacagg cgctaatttt agcgatgtgg tgaataaatt gaaaagcgaa
     3481 aatattatta aagatgtaac tgcatttgaa tggctaagca agaaaaaaaa atacaccagc
     3541 aaagtgcgcc ccggttgtta cagaatcaca aaaggcatga ccaacgaagc cattgtgaat
     3601 ttattgcgat cgggtattca acaacctgta gaagtaaaat ttaataatct acgtataccg
     3661 tcacagttag ctggtgcaat tgcagggcaa atagaagccg atagtttgtc tttgtacaaa
     3721 atgcttgttg acgaaaatgt tgccaaatca tacggcttta atgttaatag ctttatcgcc
     3781 atgtacgtgc caaacactta tgagttttac tggaatacca gtgccgaagg atttgtaaag
     3841 cgtatggcaa aagaatataa atcgttttgg aacatatcgc gcaaagcaaa agcaaaaaaa
     3901 tggaacctta ccgaaagtga aattaccaca ctggcatcta ttgtacagga agaaacaaat
     3961 atggccaccg acaaaccaat tgttgcagga gtatatctta atcgtttgaa aaaaaatatc
     4021 cctctcgaag cagacccaac tttaaaattt gctttacaag attttacaat taaaagaatt
     4081 ttaaatgtgg ataaagaggt aatttcgcct tacaacactt ataaaaatca aggtttacca
     4141 cccgggccca tttgtatacc cgatgtttca acaattgatg cggtgctaaa tgctaccgaa
     4201 catgattaca tatttttttg tgctaaggaa gatttttcgg gctattcgaa ttttgcagtt
     4261 acaaatgccg agcatgaaat aaatgcaaaa aaataccaac aagcattgaa caaaatgaat
     4321 ataaaacgat aacatgcatt acaaaatttt ttcgcaatcg cctcatacac attttattaa
     4381 tatagaatgc actattgaaa atattgataa agatgagttg atattgaagt tgcctgtatg
     4441 gcgaccaggc cgttatgaac gtgtgaactt tgcaaaaaat attggttcct ttaatattgt
     4501 tgattcacat ggacatgatg taccgtttta caaaaaagaa agttccattt gggttgttga
     4561 tacaaagact gcctcatcgt taaaaataat ttacacctac tttgccaatc agcccgatgc
     4621 aggtgcatgc ttttgcaaca acgaaatatt atacatcaat cctatacatt gctgtgtgta
     4681 cttagatgaa cgtatgcctt tgcagcacac catggatttt ataacgaatg atgactggca
     4741 aactgcaaca tcgttatcga aagacaccaa caacttttat gttgccaacg attttcatga
     4801 acttgttgat agccccatta ttgctgcaaa gcaaatgcag caccactcct actctacgca
     4861 caatacgcta tttaatattt ggattcaggg cgaatttgaa aacaatatcg aaaaattaat
     4921 tgccgatttt aaactgtttt cagatttgca actgaaagta atgcgcgata ttcctgtgag
     4981 cgaatatcac tttctgatta ttataactcc gcataaattt tatcatggcg tagaacattt
     5041 aaaaagcacc gtgttggcaa taggtaatgc agaagaattg ttgaaagacg aatggtacaa
     5101 cgaattgatt ggagtagcct cgcacgaatt gtttcatgta tggaatgtaa aaaccatacg
     5161 ccctgccgaa atgttgcctt ataaatatga taccgaaaat tatgctgcaa cagggtatgt
     5221 atacgaaggc attacaacct actatggcga tttattcctt gcccgttgcg aatatttaaa
     5281 tcatcatcaa tatttagaag aaataaacac gcggctcaat aagcactatc acaacgaagg
     5341 gcggttaaac tattcggtaa tgcaatcatc gttcgataca tggctcgatg gatatgtgcc
     5401 gggggtaccg ggtcgtaaaa cttcgatata cgatgaaggt tcgcttatag ctttaatact
     5461 cgatttacat attatacatt atagcaatgc caccaaaagt ctcgatgatg tgatgcaggt
     5521 gttatacaac gattttggaa aaaataattt aggatacaaa catcatgata tacaacgggt
     5581 gtgcgaaaat attgccgggc tatcgtttgc caatattttt aacaacctta tcgaaaaaaa
     5641 tatcagctat gaaaccgcac tcgatcaggc gttaaatttt gtaggttgta aaattacgtt
     5701 agtgcaatcg aaaaatagtt gcgaaaaaaa ctatggcatg aaactgattg atgaacataa
     5761 aaacgaaaga tggcgcgtta ctgcagtaca gaaaaattct ccggctgata taggcggcat
     5821 tgccgtgaac gatgtgctta ccaatgcctg ctatagcatt atctgcgata acaatatcaa
     5881 tcatattttt gaccatgaca aatgtgaagt atattttgat tcttatatca aacagcgata
     5941 cacagtattg catgcaaatg gaaaaacatt ttttgatatg ttgaaagtag aatcgataga
     6001 aaccaaaaca gaacagcaaa ccaagcttta taattattgg ttaggagaaa gaaattaata
     6061 caaaaaaaag gctgccaaaa attgacagcc tttcttttaa gttcaataaa acaacaaaat
     6121 caattcacct tagaaaagcg tttggttacc atacccgctt ttgtttcaat acgcataaag
     6181 taaattccat tgttaagatt acttgtatcg aagttcagtc tatacactcc tgctttcata
     6241 tcactttgat tcatgagtac attcatttgt tttccatcga tacctacaac agatattttc
     6301 aatggactat catcgttcaa tgtatatgat acattcacag tgttatgcac tggtacagga
     6361 tacaattcga aatcatcgat cttgaatgat tgattttgag cttgcagaat atcttcaact
     6421 ttatcgtcca taggtccagc ataggaaccg ctcaattttt gaattggaaa tggattcgat
     6481 gtaccggtgc aaccatcaat gtaggcatga agtaaactac catcggttat ttcaaaacca
     6541 tcaaccaaat caatataact gccggcattg taattacaat cggtgcctgt gccaccataa
     6601 agatgcatgc tcgaagaaat actactactg gcttcgaaat aaaaatatcc ttgatcttgg
     6661 taatttgaag ttgaaagtga atatccagaa gggcaacctg aataagtatc agatttccaa
     6721 aatccacgac cataagttgc agcaaccagt tgattagttg aagtgtgaat ttcaagctct
     6781 cttacaataa cattcggcaa tccattcata aacggaatcc atgttcccaa tgcatcgtca
     6841 cgataataaa caccaacatc ggtaccaaca taaataccat cggccgagcc attgtgatac
     6901 agtatgcaat taacaggaat gtttggcaat cctgtcgata tgtttgtcca cgtaacccct
     6961 ccgtcaattg tttcaaacac tttattagca gcactaaatc cactaaaaga tacgaatgca
     7021 tttaatgagt ttgtattgtc aactgtgatg ctggtaatag atgcagatgc taccggtaag
     7081 gtgcctgtaa catttgtaaa agtaggagca gcagcggtac aattatcgct gcgccatacc
     7141 ttactcgatg taacataata aagtcttgac gaattgctag ggcaggctgt taagtcaact
     7201 ccggttccta atgaaccgcc aatctgagtc catgccgttc cactattcgt actgcgatag
     7261 catttatcgt aaccaatata cattcttgca tcatttgcag ggtcaattat gtatggtgtg
     7321 ttccatccgg caccagtagc acccggtggt actatactgg taaaagtaga accgctattg
     7381 gtagaacgcc ttataccacc attttgagac tcgatgaaaa gtgttgtagg agttgattct
     7441 ctataagcaa cctcaaaacc atcggcacca taaaagtgtg taaacgtggc accattcaat
     7501 tgattgctac cattgtcttg cgcaccacca agtatttttg cagagtttga ttgcaacaat
     7561 cctaaattat aaaattgcgt aatgcctaat cccgtagaac gatcagccca aataactcct
     7621 ttatccaacg agcggtaaat accaccatcg cttccgcaat aaagggaagt accaaaatac
     7681 tccaaagcgt gaatatcggc atgagtatat tgtgctggtg tagcatcttc tacccagtat
     7741 gaagagttag tccatgtagc tccaaagtca gtggaacgcc atgtattaat gcctccaata
     7801 ataacattag caacatttga tggatcaaca gcaattgcca gatcgtacga agattgtgat
     7861 tcaaaatccc cacctgcaat atcataacca agaatgtttg gagtacttga ttttaaacca
     7921 aagtttaaac cactatcgaa ggaacgatac aaccccatga atccggaact ttttccataa
     7981 agcaaataaa cataagctgt attatttgcc gaaacaccaa ttgcaattct attagaacca
     8041 ctcgtaggca aacctactgc accggttacc caagtagcac ctgcatctgt cgataaataa
     8101 aatgttgtag tgctcactaa ataaagtgta gcaggtgttc ccggcttaaa ttcgcaatcg
     8161 cgaaacgagc cggattttac caacgtccac gatgtacctg catttgcagt tttatataat
     8221 ccatttgtag ttacagcata tagtatggaa ctgttggtag gatctaccat aagtttatac
     8281 cctttcacat aatcggtagg agtgaacgat aagcctgttt ttttccatgt aactcctcca
     8341 ttggtagttt ttaaaacacc tacagaatat gtatcactac catctccatc accggttagg
     8401 atatatattg tattggtact gatgaccaca atgcccgata ccccaaggcg tggaataccg
     8461 tccgacagtg atgtccaggc attgccgctt gtagtacttt tccaaattcc accggaagga
     8521 gtacccacaa aaatattact tgccagtgtt ggatgtctag caatgcaatt tacacgacct
     8581 actccaccat tatatccgct gttacctttt acgtgtgtgg taggcccaac aaatgaccag
     8641 tatccagagt gtgtaaaagt tgtattggga tcaccttttt tagcattgtc ttcgtaagca
     8701 aaatattctt cccatacttt ggcttcaatg ttcattattt ttcctgatgg atatacgcgc
     8761 ggagccatat aatcctccca gcgcttgtat tgcttatagc cactgccttg cccggttgca
     8821 tgttctgcat aataatcatt caactctttt tgaatggtat aaaaattatc accggcttta
     8881 atttcttcaa acttttcgcg gaacctgttt tgcgcgaagt ttataccggg cagcaaccat
     8941 gctcccagta aaatagtaaa tattattttc ttc
//
LOCUS       DAORQA010000062         8837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2092_length_8837_cov_16.272603, whole genome shotgun sequence.
ACCESSION   DAORQA010000062 DAORQA010000000
VERSION     DAORQA010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2092_length_8837_cov_16.272603"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..1286
                     /locus_tag="PK736_05100"
     CDS             303..1286
                     /locus_tag="PK736_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32801.1"
                     /translation="MNILVMGSLDTTNENCAEFLQFFAAEIVGNGHKLLNGNRNEMDK
                     VLAGNMNKLLLEKGLDPHKNIISYVEPGNTPMHDFGSILKSRCTSWGSLASVDLEVPE
                     TVQIADVVFVVGGKEGTNCAANWARILNKPLLPISNFDGAAAEIFAQELSQFDRKYSM
                     RIDKSDYEILNQITTDASRIAKDSVALAARIIFSNQVLVIMSYSSDPKLEDAYDSIET
                     ICTENKYKCLRVNESNQTERIVPEIFRQMGRSAFIIADISEAKPNVYYELGYAQGLQK
                     DLIITAAKDTELPFDVADFPVIMWDSQKYLKDKLRDKITLLAKKHGRIFTE"
     gene            complement(1532..1843)
                     /locus_tag="PK736_05105"
     CDS             complement(1532..1843)
                     /locus_tag="PK736_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32802.1"
                     /translation="MEDISTTNFSFLKTFCVIVLTANVICFFVTMDPDYVGLYVAMFV
                     MAPVNFLFIIIGLAKVVVLQKRKVQLNYVLHYTVVILLPALLEMALINLVDVFAPKGG
                     C"
     gene            complement(1919..2737)
                     /locus_tag="PK736_05110"
     CDS             complement(1919..2737)
                     /locus_tag="PK736_05110"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HRC32803.1"
                     /translation="MKKNISVLFAILFFTIACNNDIKKKEKTVVINNAVPILGYEIIN
                     TFPHDTNSFTEGLLVHDGKLFESTGATSQLPQTRSLFGIVDLQTGKIDVKAELDNAKY
                     FGEGIVIINNKIYQLTYQTKIGFIYDAKTYKKIGEFTFPSNEGWGFTTDGTYLIMSDG
                     TNMLTYLDPENLSKVKSIAVSENGYALDYLNELEYINGYVYANVWGKNTIVKIDTASG
                     KVISKMDLTSLAEEAKKISDGSKEMNGIAYDAANNKIFVTGKMWPNIYEIKFNH"
     gene            complement(2761..3222)
                     /locus_tag="PK736_05115"
     CDS             complement(2761..3222)
                     /locus_tag="PK736_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC32804.1"
                     /translation="MTIRKYSDTNRDEILKLLRLNTPQFFAASEEADLIYYLENHAHN
                     FYVAIEENKILGCGGFNLADDKSVMRISWDIVHPSSQGRGVGTALTLFRINKIKEIES
                     IKTISVRTSQHVYKFYERFGFEIKEKVKDFWAPGFDMYRMECSVDSINVEL"
     gene            complement(3226..>3427)
                     /locus_tag="PK736_05120"
     CDS             complement(3226..>3427)
                     /locus_tag="PK736_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32805.1"
                     /translation="YISYCDELVELVSESLIPIKKAKEHENAETNSLQLISESQLSLV
                     FYFCWQSENAIVHNGWCKINST"
     assembly_gap    3428..3466
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3748..>8837
                     /locus_tag="PK736_05125"
     CDS             3748..>8837
                     /locus_tag="PK736_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32806.1"
                     /translation="MSSQNFFKLYVLAIVLLLSSNKTCCAQNWTQLGRGAGGQIRALY
                     LHNNGNSFNLYVGSDVAGVWKTEPIVSLTNNIEYQYKYISNSDIMRFTNKFYRPMRAS
                     YTSNYLFVASYHGIHRIDLNAQDGNMVHILDFDYSWVSDIYICDTIQNQHTIYFTTGN
                     TRVNSDVVQYPKTKNILDFYWGSLNESENFIDRNLLKGIRLKGMPKGGDAYCLHIDDN
                     STALPNDDSILLGTQIGLWHFSYNDAVNAEMLADKTLSANIHMASNPNGTNDPYIVTS
                     ITKIPNGSAFLITVSNQGVFEWDGNSWYTRGDTILIGDQDSVNYQNYNSWQNVPVSAK
                     ALNQILPIIKNDTLQGYLLLNENHDINNNRFCGIYYCDKKINTNWIWKTLSVTNTNDW
                     GWNLALPAANINSAALTPDNKLIIGKNGILHISSNSDITQATNTQWQQIYTTLATSSC
                     SGNNYWNNKGLVNTVVRSMYVVGNNLLAGEFDRGLLLGDRVNNFVAINRKGNSTCEIL
                     KFTSPQSNGCSDTSSPSISDCTFITSKIDTTLNGQETLYACFGEGNSSEQGRGYVMSR
                     IKNVIPPLWTQLGNTLCGDPIKIIFDSQNNIFCILNKGNNYNLYYLYNGNWNLCSMVH
                     NGASVNSDITNALITKIGNDDYIYFIKVENSKGIYRLKKGSGFGNWNDSITSGNTRIL
                     TSRVGNQLELMNYNFGKKIFIGLQNSTDNANNLYRCNLNLVAEPPINNNNIACDSLNV
                     AKNIFSTPGNDETSSGVQSISVNNDSRIIYVSLLKQDEITKISYPHIYRNTYNIGNGA
                     LGSCWSEITNNLPSKNLQILMSKGSSTSETLYGALRGMGEWKCDICMVISCSILKPTC
                     NLNDGSSTVISITGSPTTYLWSNGQTTVTAAGLSPGTYTVTATNNSGCSATASVIIEG
                     FTVSANVINSCANNTGTITTTLKGGKSPFNYIWNNAATTSAISNLSAGSYTVTVTDST
                     GCNISNSFTVINSSGIDSFVVASAQTACSGSTIGYVSAINVTGGIAPYSYLWSDGAIT
                     SLNNVSFGSYTVTISDAAGCSATGTAILNPLNVSCTSSSICGVANASAQAEGGTPPYN
                     YQWSTGGTMQTISNVPPGIYTVTVTDSNSCTKSSTCLIANSAGIDSLNCIVGNINCYG
                     DTNGWINVFSIAGGTAPFTYMWSNAFTSSSISGLGEGTYTVTITDANGCIATCESIIT
                     SPSVLSTSCSAMPLSCFGINDGTVDVAASGGTPGYNYLWSNSITTSAISGLAAGTYTV
                     TVTDVNGCIATCESIITSPSALSISCSAMPASCFETNNGTVDVAASGGTPGYNYLWSN
                     SITTSAISGLAAGTYTVTVTDVNGCIATCESIITSPSALSISCSAMPASCFETNNGTV
                     DVAASGGTPGYNYLWSNALTTSYISGLAAGTYTLTVTDANGCIATCESIIAALTKMCT
                     AMPVSCFGINDGTTAVAVSGGTPVYNYMWNNAATTSSISGLEAGTYTVTVTDANGCIA
                     TCESIITSPSALTTSCSAMPASCFETNNGTSDVAAIGGTPAYYYLWSNAITTSAVSGL
                     AAGTYTVTVTDANGCTASCEAIIMQPSNALSAIVSATIGLCNGDTSGTANIIAAGGTA
                     GYSYLWNTGASTSTIANLAAGTYTVIVTDSNGCTDSNAVVINNYDTVSAVITQTNILC
                     GATSTGIISVNPTSGVAPFLFSLDSGNFV"
BASE COUNT         2848 a         1580 c         1601 g         2769 t
ORIGIN      
        1 ttattagccg gtcatgcttg ccgaatgata caacaaattc aatttgcact tttaataaag
       61 gagaagccat ttttcttttt caattatttt ttatttgaat ccattttaaa ataatatgat
      121 tgcctcacat gaaaatgctt ttttttaaaa aaagaaattg cccaattcac tctcaatttc
      181 acgtcaaaat atatttttta caaacgacaa atttaattgt ctttcggtta gtaacgctat
      241 ataaaatata gtttctatat tgcagattct atttagttaa ctaacctttt aaaaataatt
      301 atatgaatat tcttgtgatg ggctcgctgg acacaaccaa tgaaaattgt gctgagtttt
      361 tacaattttt tgcagctgaa atagttggga atggccacaa acttttaaat ggaaaccgca
      421 acgaaatgga taaggttttg gccggcaata tgaataaatt gctacttgaa aaaggactcg
      481 atccccataa aaatattata agctatgttg aaccgggcaa tacccccatg cacgattttg
      541 gttccatttt aaagtcgcgt tgtaccagtt ggggcagctt agcctctgtt gaccttgaag
      601 tgcccgaaac agtacaaatt gctgatgtgg tttttgtggt gggaggaaaa gaaggaacca
      661 actgcgctgc aaattgggcg cgaatattaa ataagccact ccttccaata agcaattttg
      721 atggtgctgc tgccgaaatt tttgcacagg aacttagtca gttcgataga aaatatagca
      781 tgcgtataga caagtccgat tacgaaatat tgaatcaaat aacaactgat gcatcacgaa
      841 tagcaaaaga ttcggttgca ttggctgcac gaattatttt ttcgaatcag gtgttggtga
      901 taatgtccta ttcgagcgat ccgaaactcg aagatgctta cgactcaatt gaaacaattt
      961 gtactgagaa taaatataaa tgtttgcgtg tgaacgaaag caaccaaacg gaacgaatag
     1021 tacccgaaat ttttaggcaa atggggcgat cggcatttat aattgcagat ataagtgaag
     1081 ccaagccgaa tgtatattac gaactgggat atgcacaagg cttgcaaaaa gacttaatta
     1141 taacagctgc caaagacacc gaactgccat tcgatgttgc tgattttcct gttataatgt
     1201 gggatagcca aaagtacctg aaagacaaac tcagagataa aattactttg cttgcaaaaa
     1261 agcatgggcg catatttacc gagtagcaaa atatcagaat cacattcttg caaatagcag
     1321 cgcttagcac ttttgtgaaa caacttgatt ttatttcgaa gaatatacct gttcacgtat
     1381 caagaaaatt tacttgactc aacatgttgt tttgacattt tttttgtttc attatttgca
     1441 aaataaaaaa agcaccgtgt aaaaactatt atttattaaa agttgaatcg gtaaacttgt
     1501 atacaactaa aacaagtttt attattctat ttcaacatcc gcctttgggt gcaaacacat
     1561 ccaccaaatt aattaaagcc atttctagta aagccggaag caatattact accgtataat
     1621 ggagtacata atttaattga acttttcgct tttgtaaaac aacaactttt gccaagccta
     1681 taattataaa taaaaaattg accggtgcca ttacaaacat cgctacatac aacccaacat
     1741 aatcaggatc catagtaaca aaaaagcaaa taacatttgc tgttaacact attacacaaa
     1801 atgtttttaa gaaactaaaa ttagtggtcg aaatatcctc cattattttt atttagcatg
     1861 ttaatttaat ttgttaatca attttaaaaa tgtaagtgtt gctacaaaat tatgcagatt
     1921 aatgattaaa ctttatctca taaatatttg gccacatctt tccggttaca aatattttat
     1981 tgttggctgc atcataagca atgccattca tttctttgct gccatcggat attttttttg
     2041 cctcttcggc caatgaggtt aaatccattt tcgatatcac tttaccactt gcagtatcaa
     2101 tttttacaat agtatttttg ccccatacat tggcatatac ataaccattg atgtattcaa
     2161 gttcgtttaa gtaatcgaga gcatagccat tctccgatac ggcaattgat tttactttgc
     2221 ttaaattttc gggatcgaga tacgtcaaca tgttagtacc atcgctcata attaaatatg
     2281 taccatcggt tgtaaagccc catccttcat tcgatggaaa agtaaactcg ccaatttttt
     2341 tatatgtttt tgcgtcataa ataaatccaa ttttagtttg atatgtaagc tgataaattt
     2401 tgttgtttat aataactatg ccttcaccaa agtattttgc gttatccagt tcggccttca
     2461 catctatttt tccggtttgc aaatcaacaa tgccaaaaag agatcttgtt tgtggcaatt
     2521 gcgaagtggc accggtactc tcaaataatt ttccatcatg caccaataaa ccttcggtaa
     2581 aagaatttgt atcgtgagga aatgtattga tgatttcgta tccaagaatt ggaacagcgt
     2641 tatttataac aacggttttt tcttttttct taatatcgtt gttacatgct atggtgaaaa
     2701 acaaaatcgc aaataaaaca gaaatatttt ttttcattcg agcgattatt cgttttggtt
     2761 ttacaactca acattgattg aatccacact gcattccatt ctatacatat cgaaacccgg
     2821 agcccaaaaa tcttttactt tctcttttat ttcgaaaccg aaacgctcat aaaatttgta
     2881 tacatgctgt gatgtgcgca ccgaaattgt tttgatactt tctatttcct ttattttatt
     2941 aatacgaaat aatgttaacg ctgttcccac tcccctaccc tgactcgaag ggtgaacaat
     3001 gtcccaggat atgcgcatta ctgatttgtc atcggccaaa ttaaaaccac cacatccaag
     3061 tattttattt tcttcaatgg caacataaaa gttgtgggca tggttttcta aataataaat
     3121 caaatcagcc tcttcacttg cagcaaaaaa ctgtggagtg ttaagccgaa gtaattttaa
     3181 tatttcgtct ctattggtat cgctatattt tctgattgtc attgattatg tcgaatttat
     3241 tttgcaccaa ccattatgca cgatagcgtt ttcagattgc caacagaaat aaaaaacaag
     3301 agatagttgg gactctgaaa tgagctgcag agaattagtt tcagcgttct catgctcttt
     3361 tgctttttta attggtatga gtgactctga aactaattca actaattcat cacagtaact
     3421 aatatacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaca gcagcaattg
     3481 atttagtaaa gttcattgat ttaattgttt gattaatttc aacaaacgta ataattttta
     3541 tgacacaacc acttacaaaa acaatcgcat aaaatttcaa tttttgtaaa taatagagat
     3601 atcaatttgt attgaaattg taactatgaa tgtgtttgtt tacaatatag ttgctaatta
     3661 aatgtaaaat gaggtaaact atttgaagcg gcagtcaact tgcactacat tgcacgaaat
     3721 attattaagt acaacttttt aaaagcaatg agctcacaga actttttcaa actttatgtt
     3781 ttagcaatcg ttcttctttt gtcatcaaac aaaacatgtt gtgcgcaaaa ctggacacag
     3841 cttgggcgtg gtgcaggagg gcaaataagg gcgttgtatt tacataacaa tggaaatagc
     3901 tttaaccttt atgttggttc tgatgtagcg ggagtgtgga aaactgagcc aatagtttct
     3961 cttacaaata atattgaata tcaatacaag tatatcagca attcggatat catgcggttt
     4021 acaaataaat tttacagacc tatgcgtgcc agctatacat caaattattt atttgttgca
     4081 agttatcatg gaatacaccg aatagatttg aatgctcagg atggtaacat ggttcacatt
     4141 ttagatttcg attactcgtg ggtgagcgac atttacattt gcgatactat ccaaaatcaa
     4201 cataccattt attttactac cggcaacaca agagttaata gcgatgttgt tcaatatcca
     4261 aaaacaaaaa acattcttga cttttattgg ggctcactaa acgagtcgga aaattttatt
     4321 gaccgcaatt tgcttaaggg aattaggtta aagggaatgc caaaaggtgg cgatgcttat
     4381 tgcttacata ttgatgacaa cagcactgct ttgccaaacg atgacagcat attactggga
     4441 acacaaatcg gcttatggca ttttagttat aacgatgctg tgaatgctga aatgctcgca
     4501 gataaaacct tatctgcaaa cattcacatg gcttccaacc ccaatggaac caacgatccg
     4561 tatattgtta caagcataac aaaaatacca aatggatcag catttcttat aactgtttct
     4621 aatcaaggag tttttgaatg ggatggaaat tcgtggtata ccagaggtga tactatttta
     4681 attggcgatc aggattcagt caattatcag aattataatt catggcaaaa tgtaccagtt
     4741 tctgctaaag cattaaatca aattctaccg atcattaaaa acgatacatt acaaggatac
     4801 ttacttttaa atgagaacca cgacataaat aataatcgat tctgtggaat atattattgt
     4861 gataaaaaaa taaatacaaa ctggatatgg aaaacattgt cagttacaaa cacaaatgat
     4921 tggggatgga atttagcatt gcccgctgca aatattaaca gtgctgcact tacacccgac
     4981 aataaattaa ttattggtaa aaatggtatt ttacatattt catcaaactc agatattacc
     5041 caagcaacaa atacgcaatg gcaacaaatt tatacaacac ttgcaacttc aagttgttcg
     5101 ggcaataatt attggaataa taaagggctt gtcaatacag tggtacgaag catgtacgta
     5161 gttggcaata atttgttggc aggtgaattt gacagaggtt tgcttttggg tgatagagtc
     5221 aataattttg tggccatcaa tagaaaaggt aacagcactt gcgaaatcct gaaatttact
     5281 tctccacaat ctaacggatg tagtgacact tcttcaccct caatatctga ttgtactttt
     5341 ataacatcaa aaatcgacac caccttaaac ggacaggaaa cactctatgc ttgctttggt
     5401 gaaggtaatt caagcgaaca aggcagaggt tatgtaatgt cacgaataaa aaatgtgatt
     5461 cctccattat ggacacaact tggtaataca ctttgtggcg acccaataaa aattattttc
     5521 gatagccaaa acaatatttt ttgcatattg aataaaggca ataactacaa cctttattat
     5581 ttatataatg gtaattggaa tttatgcagt atggtgcata atggtgcatc cgtcaactct
     5641 gatataacga atgcattaat tacaaaaatt ggcaacgatg attacatcta ttttatcaag
     5701 gtggaaaatt ccaaaggtat ttacagatta aaaaaagggt caggatttgg aaactggaat
     5761 gatagtataa cctcagggaa tacacgaatt ttaacctcaa gagttggaaa tcaactggaa
     5821 ttgatgaatt ataattttgg aaaaaaaata tttattggat tgcaaaacag cacggataat
     5881 gcaaacaatt tatacaggtg caatttaaac cttgtagcag aacctccaat aaataacaat
     5941 aacattgcgt gcgattcgct caatgttgca aaaaatattt tttctactcc tggtaatgat
     6001 gaaaccagca gcggtgtaca atcaatttct gtaaataatg attcgagaat tatatatgtt
     6061 tcgttattga aacaagatga aataactaag atatcgtatc cgcatattta ccgcaacacg
     6121 tataatatag gtaatggcgc tttgggttcc tgttggtcgg aaattactaa taatttacca
     6181 agcaaaaatc ttcagatttt aatgagtaaa ggttctagca cttcagaaac tttgtatggc
     6241 gcattacgtg ggatgggcga atggaaatgt gatatatgta tggttatatc ttgttcgata
     6301 ttaaaaccta cctgcaatct aaatgatggt tcaagcactg ttattagtat taccggaagt
     6361 cctactacat acttatggag caatgggcaa acaaccgtta ccgctgctgg gctttcacct
     6421 ggaacgtata cagttaccgc tacaaacaat agcggttgta gcgcaactgc ttcggttatt
     6481 attgagggtt ttacggtttc ggcaaacgta attaatagtt gcgctaataa tactggcact
     6541 ataactacta ctttaaaagg tggtaaatcg cctttcaatt atatttggaa caatgctgcc
     6601 actacatcag caatatcaaa tctttctgcg gggtcgtata ctgtcaccgt tactgatagt
     6661 accggatgca atatatccaa ttcctttaca gtaataaatt caagtggtat agattcattc
     6721 gtggttgcat cggcccaaac tgcttgttcg ggttccacca ttggttatgt ttcggcaata
     6781 aatgttacag gcggaatagc accttactcg tatttgtgga gtgatggagc tataacttca
     6841 ctgaacaatg taagttttgg ttcgtatacg gttaccatta gtgatgctgc aggatgttct
     6901 gccaccggca ctgcaatttt aaacccatta aacgtttcct gcacttcatc ctctatttgt
     6961 ggtgtagcga acgcatcggc acaagcggaa ggaggtacgc caccttacaa ttatcaatgg
     7021 agcacaggtg gcacaatgca aactatttcg aatgtacccc cgggaattta cacagttaca
     7081 gttaccgatt cgaacagttg cacaaagtct tcaacttgtt taattgcaaa ttctgcagga
     7141 atagattctt tgaattgtat tgttgggaat attaattgtt atggagatac gaatggatgg
     7201 ataaatgtgt tttcaattgc cgggggtacc gctccattca cttacatgtg gagcaacgca
     7261 ttcacaagtt cttctatcag cggattgggt gaaggtacgt atactgtaac cataaccgat
     7321 gcaaatggtt gcattgcaac atgcgaatct ataattactt caccatctgt tttatccaca
     7381 agttgcagtg caatgccttt aagctgcttt ggtataaatg acggaactgt tgatgttgca
     7441 gcaagtggcg gcactccggg ttacaactac ttgtggagca attcaataac gacttctgct
     7501 atcagcggat tagccgcagg tacgtatact gtaactgtaa ccgatgtaaa tggctgcatt
     7561 gcaacatgcg aatctatcat tacttcgcca tcagctttat ctataagttg tagtgcaatg
     7621 cctgcgagtt gttttgagac taataacgga actgttgatg ttgcagcaag tggcggcact
     7681 ccgggttaca actacttgtg gagcaattca ataacgactt ctgctatcag cggattagcc
     7741 gcaggtacgt atactgtaac tgtaaccgat gtaaatggct gcattgcaac atgcgaatct
     7801 atcattactt cgccatcagc tttatctata agttgtagtg caatgcctgc gagttgtttt
     7861 gagactaata acggaactgt tgatgttgca gcaagtggcg gcactccggg ttacaactac
     7921 ttgtggagta atgcattaac aacttcttat atcagtggat tagccgcagg tacatatact
     7981 ttaaccgtaa ccgatgcaaa tggttgcatt gcaacatgcg aatcaataat tgctgcacta
     8041 actaaaatgt gcactgcaat gcctgttagt tgctttggaa ttaatgatgg aacaactgca
     8101 gttgcagtaa gtggaggcac accggtttac aactacatgt ggaacaatgc agcaactact
     8161 tcctctatta gtggattaga agcaggcaca tatacagtaa ccgtaaccga tgcaaatggt
     8221 tgcattgcaa catgcgaatc tataattact tcaccatctg cattaactac aagttgtagt
     8281 gcaatgcctg cgagttgttt cgagactaat aacggaactt ctgatgttgc agcaattggc
     8341 ggcactccgg cttactatta tttatggagc aatgcaataa cgacttctgc tgtaagtgga
     8401 ttagccgcag gtacgtatac tgtaaccgta accgatgcaa atggttgcac tgcatcttgt
     8461 gaggcaataa taatgcaacc gtccaatgca ttatctgcta tagtttcagc aacaattggg
     8521 ctttgtaatg gggacaccag tggcaccgca aacataattg ctgcaggtgg cacagcaggt
     8581 tactcttatt tgtggaatac aggagcttct acttcaacta ttgctaattt ggccgcaggc
     8641 acttatacag ttatagtaac tgatagtaac ggttgtacag atagtaatgc tgttgtaata
     8701 aataattatg ataccgtaag cgcagttatc acacagacaa acatactttg cggtgctaca
     8761 tccaccggaa taataagtgt aaatcctaca agcggtgttg caccctttct cttttctctg
     8821 gattcaggca attttgt
//
LOCUS       DAORQA010000063         8445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2220_length_8445_cov_8.422169, whole genome shotgun sequence.
ACCESSION   DAORQA010000063 DAORQA010000000
VERSION     DAORQA010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8445
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2220_length_8445_cov_8.422169"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..>601)
                     /locus_tag="PK736_05130"
     CDS             complement(98..>601)
                     /locus_tag="PK736_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRC32807.1"
                     /translation="ALKKYVKGADFKNIKFETLENQIEIKYGKIIIPNMEIRSTAMNL
                     YASGTHTFTNEIDYKIKMQLSEVLGRKVKNLNTEFGIIEEDGLGKPNIFVSMKGPIAN
                     PVVKYDRKAVESHIAQSAKQEKQNLKVILNKEFGLFKKDSTVIKANTTPERKKKKQEL
                     IIDTDPF"
     assembly_gap    602..616
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<617..2632)
                     /locus_tag="PK736_05135"
     CDS             complement(<617..2632)
                     /locus_tag="PK736_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32808.1"
                     /translation="MSEQDIPGNKFRWLKYSLYTLGGVILTFILAAVVITFFFEDKIK
                     QSVVGAINSQLNTKVEVKNINFSLLSKFPKASVEFTDVYIHSTPPFASTDTLAFAKQF
                     FLMFNVFDLFGSEYKIHTAEIMNACIYMKINKAGVHNFDITKKTNTAGNDNISLQIKA
                     LQLTNVLYDYDDKQQSQKYALLLQNGEASLGYANNTIFVEGNTQSNIYTIQWNNNNYI
                     DSLQAGIKLKMEIASNGNKIIIRPSQIETENMTLQTQGQIDNKPKSTGIQLSFNSKNA
                     NLLSLLKLVPSSQRAMLLQYDTEGNVTFDCKIAGDAGAGIQPDIDCTFNATDATVFNT
                     DAKVGLQQIKLKGAYSNHFAGKKNNDCISIKEFSCKLNNAPIKGQAQILGFDNTHLIF
                     NAEGRFDLAQVKDLFAIDTVQSITGQAMAKIKFDGFVNDKSSYNTSGNIEISQAAFQF
                     ANTTTSWKNINAMLLLNDNTLQIEECSFNSENSDFSFKGYCENFPGCIFTQNKNISIQ
                     SSLTCNMLNLDEFIITENSDVKSDTAMPAINLPAWVESSLMLSIKQVSYKKFSAKNVV
                     GNIDLLDKIIFAQNIRLQAFGGVVMVEGKIDASNSDHIIIACDAGITKISIKQLFTQT
                     ANFGQKVITDNNLNGIADATVQLSTWWSPNLEINMSKIMAVSDVTINN"
     gene            complement(2787..3287)
                     /locus_tag="PK736_05140"
     CDS             complement(2787..3287)
                     /locus_tag="PK736_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002662342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRC32809.1"
                     /translation="MTTVKDQNTDFEVRSLDTMCVNESHLQHKWYHSIKYLFVGIGFG
                     IILIKAEVISWFRIQEMFRLQSFHMYGVIGSAVMIGAISVWLIKKFNIKTIYGEPIEL
                     HKKEFNKGQIFGGLLFGLGWAITGACPGPLFAQIGAGTSVIIITLLSAIAGTWVYGFF
                     RDKLPH"
     gene            complement(3295..3858)
                     /locus_tag="PK736_05145"
     CDS             complement(3295..3858)
                     /locus_tag="PK736_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRC32810.1"
                     /translation="MIDLIKQPWPWYVAGPLIGLVVPTLLIMGNKTFGISSSLRHICA
                     ACMPANIAFFKYDWKKEIWNLFFVLGIILGGVLTATILANPNPVDINPTLVLELEKYG
                     ITNYNKLIPVEIFNWPALFTLKGFLMMVVGGFMIGFGTRYAGGCTSGHAIMGLSSLQW
                     PSLVATCCFMAGGFIMANLILPFILSL"
     gene            complement(3861..4496)
                     /locus_tag="PK736_05150"
     CDS             complement(3861..4496)
                     /locus_tag="PK736_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRC32811.1"
                     /translation="MTEKNIFNSLQHHMPNEENSLDENYWNTRYETKTTGWDIGEVSP
                     PLKSFIDTIENRNAAILIPGCGNCYEAEYLLSKGFTNITLIDIAPLLVEKLQNKFAGN
                     SNIKIVLGDFFDHHAKYDYIIEQTFFCALPPSLRKNYVYKMRELLNDNGVLAGVLFNR
                     SFDGGPPFGGSKEEYKNVFAPGFNIMKMETCINSIDARANTELWFEIQLKK"
     gene            complement(4503..6113)
                     /locus_tag="PK736_05155"
     CDS             complement(4503..6113)
                     /locus_tag="PK736_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32812.1"
                     /translation="MKIFFGTILILISNFVFAQNSSFTIALEPVNIAALDGIQSYAYG
                     QAAGKWLIIGGRKDGLHQRQPFAAFDASGLNTDLTVVDPVTMQKWTAPLSSLSVDLQE
                     QLSSTNMEFHQDGNYLYLIGGYGYSATMNDHITYSKLCAVDVPNVINAITNNQSFTSY
                     FRQYADTNFAVTGGHLNKINDTYYLVGGQNFIGRYNPMGPNHGPGFFQQYTNQIRKFT
                     LIDNGVSLTISNYQTITDSLAFHRRDYNVVEQILQNGNEGLTVFSGVFQTAADIPYLN
                     AVNIDSNSYSINNSFAQYYNHYHCAVLPLYSQSANEMNNVFFGGIAQYYDSAGVLVQD
                     NNVPFVKTIANVKRDNSGNMTEYKLNVEMPALLGSGSEFIANENIATFKNGVIKYDSL
                     IADTTHVGYIFGGINSTAANIFWINTGIESSASNQLFKVLVIKNNSAGISQLNHQSNN
                     GLQLQVYPNPNDGYFFIKFSLKKAKPVHLLITDANGKEVVNENLLQTTKGENIIEYKI
                     KDLTIGGVYWVALTDGDLSSAQQIIVAE"
     gene            complement(6117..6215)
                     /locus_tag="PK736_05160"
                     /pseudo
     CDS             complement(6117..6215)
                     /locus_tag="PK736_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
     gene            complement(6196..6405)
                     /locus_tag="PK736_05165"
     CDS             complement(6196..6405)
                     /locus_tag="PK736_05165"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRC32813.1"
                     /translation="MAQHNITGTEGEKIAEQFLLNAQYEIVAKNFRYQKAEVDIIAKK
                     ESILVFIEVKTRTTNYFGQTYAHAI"
     gene            complement(6421..7290)
                     /locus_tag="PK736_05170"
     CDS             complement(6421..7290)
                     /locus_tag="PK736_05170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRC32814.1"
                     /translation="MAEPQKKKFIKRLRTTLRLVVMNDDTFEEKFSLRLTPLNVFVVM
                     GTLMLTLITLTTYLIAFTSLREYIPGYADVSMKRKLVNMSLATDSLAQKIETQNKYLA
                     NLNFIVSGTLPVDKIKDTAATVAIYDSIRVLQKSAEDSLLRMKIEGQDKFDIAMDGDE
                     GSQSGIAGFYFFTPLKGTITSVFDTRKKHYGVDIVAPPDAVIKAVLDGTVIVSDFTSE
                     TGHTLAIQHRNDLISFYKHNSVLLKKPGDFVKAGDVIAIIGNSGEFTTGPHLHFELWY
                     NGTAVNPVDYIKF"
     gene            complement(7301..7993)
                     /locus_tag="PK736_05175"
     CDS             complement(7301..7993)
                     /locus_tag="PK736_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32815.1"
                     /translation="MSIRDSSINVLLVGLGYGHYLPAGDMADRFGNNSMVVGNVMFKT
                     KSQWLYGFDGTFIFSNNVNQKDLFKNIETTKGGIIGSDGKLADVIKYERGYTITFNGG
                     YLFSKKKPNPNSGFVAVAGVGFMQHKIRIETIGNTVPALSKEYRKGYDRLSNGLVLHQ
                     YFGYQYMGNKHLINFFAGIDIFEGFTAGRRSYNFDIMTADNSSRTDILIGLRAGWYLP
                     LYKKAPEKIYFY"
     gene            complement(8227..>8445)
                     /locus_tag="PK736_05180"
     CDS             complement(8227..>8445)
                     /locus_tag="PK736_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32816.1"
                     /translation="EQKVSKQKETTKAPIDWGVNRIVHNDSNLYVDKKIFCSEIKLPF
                     PIYSLRVENQRVKKVFLLFDTIKKKATR"
BASE COUNT         2542 a         1630 c         1440 g         2818 t
ORIGIN      
        1 caataaaacc tctgtggagc atattcccga tgcattttac ccttatgaat aattgcaaat
       61 aatttttctt gcttgcacat aagccaacag tcaattatta aaaaggatcg gtgtcgataa
      121 tcaactcttg ctttttcttt ttgcgttcgg gcgttgtgtt tgctttaata acagtactgt
      181 cttttttaaa caaaccaaac tctttattca atattacttt gagattttgt ttctcttgtt
      241 tggcactttg tgcaatatga ctttctactg ccttgcgatc atacttcaca acagggttag
      301 caattggccc tttcatactt acaaaaatat tcggcttgcc taatccatct tcctctataa
      361 tgccaaattc ggtattcagg ttttttactt tacggccaag cacttctgat aattgcattt
      421 ttattttata atctatttca ttagtaaaag tgtgtgtacc actggcataa agattcatgg
      481 ctgtgctgcg tatttccata ttgggaataa taatttttcc gtattttatt tctatttgat
      541 tttcgagtgt ttcaaatttt atgtttttaa aatctgcccc tttaacatac ttcttgagtg
      601 cnnnnnnnnn nnnnnnattg ttaatagtta catcgctcac ggccataatt ttactcatat
      661 ttatttcaag gttgggcgac caccatgtag atagttgcac tgttgcatcg gctataccat
      721 tcaggttatt atcagtgatt actttttgtc caaaattggc ggtttgtgta aataattgtt
      781 tgatgctgat tttagttata cctgcatcgc atgcaataat aatgtggtcg ctattggatg
      841 catctatttt tccttctacc atcaccacac caccaaatgc ctgcaggcga atattttgag
      901 caaaaataat tttgtccaaa aggtcgatgt tgcctacaac atttttggcc gaaaattttt
      961 tatacgatac ttgttttatg ctcagcatta acgaactctc gacccatgca ggtaaattaa
     1021 ttgccggcat tgcagtatca cttttcacat cggaattttc tgtaataata aactcatcga
     1081 ggttgagcat attgcaagtt aacgatgact gtatacttat gtttttattt tgagtaaaaa
     1141 tgcatccggg aaaattttcg caataccctt taaaactaaa gtcgctattt tcagaattaa
     1201 acgagcattc ctctatttgc aaagtgttgt cgtttaataa cagcatggca ttaatatttt
     1261 tccatgaagt ggttgtgttt gcaaattgaa atgctgcctg cgatatttca atgttgcccg
     1321 atgtattata gcttgactta tcatttacaa aaccatcgaa ttttattttc gccattgcct
     1381 gtccggttat tgattgtact gtgtcgatag caaacaaatc ttttacctgt gctaaatcga
     1441 aacgcccttc agcgttgaaa atcaagtgcg tattatcgaa gcctaaaatt tgcgcttgtc
     1501 ctttgatggg cgcattattc aacttacacg aaaattcctt tattgaaatg cagtcgttat
     1561 ttttcttgcc tgcaaaatgg ttggagtatg ctccctttag ttttatttgt tgcaacccta
     1621 ctttggcatc ggtattaaat actgttgcat cggttgcatt gaaagtacaa tcgatatcgg
     1681 gctgtatacc tgcaccggca tcgcctgcaa ttttacaatc gaaagtaaca ttgccttctg
     1741 tatcatattg caaaagcatt gcacgttgcg atgagggaac aagttttaat aacgaaagaa
     1801 ggttagcgtt tttactattg aacgataact gtatgcctgt gcttttaggt ttattatcta
     1861 tttgcccctg cgtttgtaag gtcatatttt ctgtttcaat ttgagaaggt cgaataatta
     1921 ttttattgcc gtttgaggct atttccattt tgagttttat gcctgcttgc aaactgtcta
     1981 tataattatt gttgttccat tgtatggtgt aaatattact ttgcgtgttg ccctcaacaa
     2041 atattgtgtt gttagcatag ccaagcgagg cttcaccatt ttgcaacagt agcgcatatt
     2101 tttgcgattg ttgtttatcg tcataatcat acaacacatt ggtgagttgc aacgctttta
     2161 tttgtaagct aatattgtcg ttaccggccg tatttgtttt tttggtaatg tcaaaattgt
     2221 gaactccggc tttatttatt ttcatgtaaa tacaggcgtt cattatttcg gctgtgtgaa
     2281 ttttatattc actgccaaat aaatcgaaca cattgaacat gagaaaaaat tgttttgcaa
     2341 acgccaatgt atcggtgctt gcaaatggag gtgtgctgtg gatatataca tcagtaaatt
     2401 caacagaggc tttagggaat ttactcagca gcgaaaagtt tatgttttta acttcaactt
     2461 tggtgttcaa ttgcgaattg atagcgccaa ccaccgactg ctttatttta tcttcgaaaa
     2521 aaaaagtaat aaccaccgct gcaagtataa aggttagtat aaccccgcca agggtataca
     2581 acgaatactt taaccaacga aatttattgc ccggaatatc ttgttcgctc atgttgcatt
     2641 atatgacgca aatgtaaatg aaaaattgtg aggcattata aattgaccga tgccatacaa
     2701 atgcaataag aaactttaaa acaaaactat tgagaaaatg taatgagtga caataaaaaa
     2761 aaacaatttt ataatttggg taatgcttaa tgaggtagtt tgtctctaaa aaatccatac
     2821 acccatgtgc cggcaattgc gcttaaaaga gtaataataa tgacagaggt gcctgcacca
     2881 atctgcgcaa acaatgggcc ggggcatgca cccgtgatag cccatccaag cccaaataac
     2941 aggccgccaa aaatttgtcc tttattaaat tcttttttat gcagttctat tggctcacca
     3001 taaatggttt ttatattaaa tttttttatc agccacaccg aaatcgcacc tatcattacc
     3061 gcactgccaa ttacaccata catgtgaaat gattgcaggc ggaacatttc ctgtatgcga
     3121 aaccaactga taacttcggc tttgattagt ataataccaa acccaatccc aacaaataaa
     3181 tatttaatgc tatggtacca tttatgttgc aggtggcttt cattcacaca cattgtatcc
     3241 aatgagcgaa cttcaaaatc cgtattttga tcttttactg tagtcatatt ttttttataa
     3301 agagagaata aaaggtaaaa ttaaattggc cataataaaa cctccggcca taaaacagca
     3361 agtagcaacc aatgaaggcc attgcaaaga ggataacccc ataatggcat ggccactggt
     3421 gcacccaccg gcatatcgtg ttccaaatcc aatcataaag ccgcctacaa ccatcattaa
     3481 aaatcctttt agtgtaaata gtgccggcca gttaaatatt tcgacaggga ttaatttatt
     3541 gtagtttgta atgccatatt tttcgagctc caaaaccaaa gtgggattta tatcaaccgg
     3601 attaggattt gccaggattg ttgcggtgag cacgccacct aaaattattc ctaacacaaa
     3661 aaataaattc caaatttctt ttttccaatc atatttgaag aatgcaatat ttgcaggcat
     3721 acacgcagca catatgtgac gcaaagagga gctaatgcca aaagttttgt tacccataat
     3781 tagtaaagtt gggactacta acccaattag tggcccggct acataccagg gccatggttg
     3841 tttaatcaaa tctatcatat ttattttttc aattgtattt caaaccataa ctcagtattg
     3901 gcacgcgcat caatggagtt gatgcacgtt tccattttca taatattaaa accgggagca
     3961 aaaacatttt tatactcctc tttgcttcca ccaaaaggag gaccgccatc aaacgaacgg
     4021 ttaaataaca caccggctaa aacaccattg tcatttaaaa gttctctcat tttgtataca
     4081 tagtttttcc gcaaagaagg cggtaaagca cagaaaaaag tttgttcaat aatgtaatcg
     4141 tatttagcat gatggtcgaa aaaatcgcca agcacaattt taatatttga attgccggca
     4201 aatttattct gtaatttttc caccaacaat ggcgctatgt ctatgagtgt aatgttggta
     4261 aatccttttg ataaaagata ttcggcttca taacaattac cacaaccggg aatcaaaatt
     4321 gcagcatttc tattttctat agtatctata aaagatttta atggtggcga tacttcgcca
     4381 atatcccatc cggtagtttt ggtttcatat cttgtattcc agtaattttc atcgaggctg
     4441 ttttcttcgt tgggcatatg atgttgtaaa gagttaaaaa tgtttttttc tgtcatactc
     4501 tattattcag ctactataat ttgttgagcc gatgataaat caccatcggt aagagccacc
     4561 caatatacac caccaatagt aaggtcttta attttatact caataatatt ttcgccctta
     4621 gttgtttgca ataaattttc attcacaact tctttaccat tagcatccgt aataagcaga
     4681 tgcacaggct ttgctttttt caatgaaaac tttataaaga aataaccatc attaggattg
     4741 ggatacactt gcaattgcaa gccgttattg ctttgatggt tgagttggct tataccggca
     4801 gaattgtttt ttataacaag caccttaaat agttgattac tcgcactgct ttcaattcct
     4861 gtattaatcc aaaaaatgtt tgcagctgta ctatttatac cgccaaaaat atatccaaca
     4921 tgagtggtgt cagcaattaa gctatcgtat tttattactc catttttaaa tgttgcaata
     4981 ttttcgttgg caataaattc cgaaccacta ccaagcaacg caggcatctc aacattaagt
     5041 ttgtactcgg tcatgtttcc gctattatct ctttttacat tagcaatggt ttttacaaaa
     5101 ggcacattat tgtcctgcac caacactcct gcactatcat agtattgtgc tatgccacca
     5161 aaaaaaacat tgttcatttc gttagcactt tgactataca acggcaaaac ggcacaatgg
     5221 taatgattgt aatactgcgc aaaagaatta ttaatgctgt aactgttact atcaatattc
     5281 accgcattga gataaggaat atcggcagcg gtttgaaaaa ctcccgaaaa aacggttagc
     5341 ccttcgttac cattttgcaa aatttgctct accacattat agtctcttct atgaaatgcg
     5401 aggctatcgg taattgtctg gtaatttgaa atagttaaag aaacaccatt atcaatgagc
     5461 gtaaattttc taatttgatt ggtgtattgc tgaaagaatc ccgggccatg atttggaccc
     5521 ataggattat atctcccaat aaaattttgc cctccaacga ggtaataagt atcgtttatt
     5581 ttattcagat gaccaccagt tactgcaaaa tttgtatcgg catattgcct gaaataggat
     5641 gtaaaggatt gattgtttgt tatagcattg ataacatttg gcacatcaac agcacataac
     5701 ttactataag taatgtggtc gttcatagtt gcactgtaac catagccacc aatcaagtat
     5761 aaatagtttc catcctggtg aaactccata tttgttgaac ttagttgttc ttgtaaatca
     5821 acagaaagcg aactaagcgg ggccgtccat ttttgcatag ttacaggatc taccacagtg
     5881 aggtcggtat ttaaaccgga agcatcgaat gctgcaaacg gttgcctttg atgcaaacca
     5941 tcttttcgtc cgccaattat taaccactta ccggcagcct gcccatatgc ataagattga
     6001 ataccatcaa gcgctgcaat atttaccggt tcgagggcaa tggtgaacga agaattttgt
     6061 gcaaaaacaa aattgcttat cagaataagt attgtcccaa aaaaaatttt catctcttaa
     6121 ctattcttat taataatatc aattatcaca tttggttgca ctacaccact ttgtcgccaa
     6181 agctgcttgc cgtttttaaa tagcatgagc gtaggtttgg ccaaagtaat tagtagtgcg
     6241 ggtcttaact tcaataaaaa caagtatcga ttctttcttt gctattatat cgacttccgc
     6301 tttttgatag cggaaatttt ttgccacaat ttcatattgg gcattcaata aaaattgctc
     6361 ggcaattttt tcgccttccg taccggttat gttatgctgt gccacaacag gttttcaata
     6421 tcaaaatttt atgtagtcaa ccgggtttac tgctgtgcca ttataccaca gttcgaaatg
     6481 taaatgtggc ccggttgtaa attcgcccga gttgcctatt atagctatta catcaccggc
     6541 tttaacaaaa tcgcccggct tttttagcaa tacggagttg tgcttataaa aagagatcaa
     6601 atcgttgcga tgctgtatgg ctaaggtatg ccctgtttcg gaagtgaaat ccgaaacgat
     6661 aactgtgcca tcgagcacag cctttatcac agcatcgggc ggggcaacaa tgtcaacacc
     6721 ataatgcttt tttctggtat cgaataccga agttattgtg ccttttaaag gagtaaaaaa
     6781 gtagaagccc gcaatgccac tttgcgaccc ttcgtctcca tccatggcaa tatcaaattt
     6841 atcttgccct tctattttca tgcgcaataa actatcctct gccgattttt gtaacaccct
     6901 tatcgagtcg tatatagcaa ccgttgcagc ggtatcttta attttatcta ccggcagtgt
     6961 tccgcttaca ataaaattta ggtttgctaa atatttgttt tgagtttcta ttttttgtgc
     7021 aagcgagtcg gtagccaaac tcatgtttac aagtttacgt ttcatactca catcggcata
     7081 gcccggtata tactcgcgca aggatgtaaa tgctataagg taagtagtaa gggtaataag
     7141 ggtaagcata agagttccca ttacaacaaa cacattgagc ggtgtgagtc gtaaagaaaa
     7201 tttttcttca aacgtatcat cattcatcac caccagacgc aaggtagtgc gaagacgttt
     7261 tataaatttt ttcttttgag gttcggccac tttatattgt ttaatagaag tatatttttt
     7321 cgggtgcttt tttataaagc ggtaaatacc aacctgcccg taaaccaatt aaaatatcgg
     7381 tacgcgaact gttatctgcc gtcataatgt cgaagttata actgcgcctg ccggctgtaa
     7441 acccttcgaa gatatcaata ccggcaaaga aatttatcaa gtgtttattt cccatatatt
     7501 ggtaaccaaa atactgatga agcaccaatc cattggaaag cctatcgtat cctttgcgat
     7561 attctttgct taaagccggt accgtgttgc caatagtttc gatgcgaatt ttatgttgca
     7621 taaagccaac acctgccaca gccacaaacc cgctattggg attaggcttc tttttcgaaa
     7681 ataaatatcc accattaaat gttatggtat atccacgttc atatttaatc acatcagcca
     7741 actttccatc gctgccaatg atgccgccct tggtagtttc aatgtttttg aataaatctt
     7801 tttgatttac attattgcta aaaataaatg taccatcaaa accataaagc cattgcgatt
     7861 ttgttttaaa cataacattg cccaccacca tagaattatt tccaaaccta tcggccatat
     7921 cgcccgcagg caaataatgc ccatagccta gacctactaa aagtacattg atagacgagt
     7981 cacgaatact cacctgacag atgccccaat ggtggaacac caaaagagtc aaggataaaa
     8041 acagccattt ttttatcatg ttgcaaaact attattaatt gtgtaaaaat catccttcct
     8101 aaaaagaggg ttatttataa gcccggtaag cagccttttt tatgctcatg caatgctttt
     8161 gataaaaaat gtttacaaaa caatatgctt tatatttcta caggagtaat agaatattga
     8221 gtttttttag cgtgttgcct tttttttaat agtatcaaag agaaggaata cttttttaac
     8281 ccgttgattt tcaactcgaa gggaataaat tggaaagggt agctttattt cactgcaaaa
     8341 aattttttta tcaacataca aattactatc attgtgaact atgcgattga ctccccaatc
     8401 aataggtgct ttagttgttt ctttttgttt gctgactttt tgttc
//
LOCUS       DAORQA010000064         8395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2234_length_8395_cov_11.722302, whole genome shotgun sequence.
ACCESSION   DAORQA010000064 DAORQA010000000
VERSION     DAORQA010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2234_length_8395_cov_11.722302"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PK736_05185"
     CDS             <1..218
                     /locus_tag="PK736_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HRC32817.1"
                     /translation="CDHMFLTKSCIETKDTIKWEDGNEYPLVKLEISNMSHPFYTGKM
                     KLIDTAGRVDKFNTRYRKAAEKATEQE"
     gene            286..1452
                     /locus_tag="PK736_05190"
     CDS             286..1452
                     /locus_tag="PK736_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013187746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HRC32818.1"
                     /translation="MNYILFDNSRQQLMPFTLSRPVCEIRVGILTIKQKWELYLNASC
                     SYECELYLQAKYPKLTASDNVYINATVFPDADLVAAIKNLKIGEALIWEGNILALRTS
                     DTISNTTSILTTEYKKPVSSISHIWHIFGMCDEQLRADFTLLTAGRNSLQLNSSNRLV
                     GDTDDLFIEEGATVNCAIINTQTGPVYIAKEAEVMEGAMIRGPFALGEHATLKMGAKI
                     YGATSIGPHCKVGGEVSNSVFFGFSNKAHDGFVGNAVIGEWCNLGADSNNSNLKNNYG
                     NVKVYDYASQNMVDTGLQFCGLFMADHAKCAINTQFNTGTVVGFASNVFGGDFPPKHI
                     PDFSWGGAKGFEKFDLQKAIALAKNVYARRSMTLTDADIAIFEAINEMAKNTKG"
     gene            1469..2155
                     /gene="trmD"
                     /locus_tag="PK736_05195"
     CDS             1469..2155
                     /gene="trmD"
                     /locus_tag="PK736_05195"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRC32819.1"
                     /translation="MRIDIITVLPELLDSPFAHSILKRGQQKGIIEVNVIPLREFATN
                     KYKSVDDYAFGGAAGMVMMCEPIANCIDHLKLQRNYDEVIYLTPDGELFSQAMANQLS
                     LKGNLIMLCGHYKGVDERIRQHYITKEISIGDYVLSGGELAAAVVSDAILRLVPGVMN
                     DETSALTDSFQDGLLSPPVYTRPAEFRGIRVPEILLSGHEKNINEWKHQQSLERTAHR
                     RPDLFAKYHS"
     gene            2246..2593
                     /gene="rplS"
                     /locus_tag="PK736_05200"
     CDS             2246..2593
                     /gene="rplS"
                     /locus_tag="PK736_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRC32820.1"
                     /translation="MNIIKSVNEQTIEKRDFPEFKAGDTITVTYKIIEGTKEREQLFQ
                     GVVIQRKGAGFTQTFTVRKMSHSVGVERIFPLNSPKIAKIEVNKRGVVRRAKLFYLRN
                     LSGKAARIEEKKM"
     gene            2801..4543
                     /locus_tag="PK736_05205"
     CDS             2801..4543
                     /locus_tag="PK736_05205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRC32821.1"
                     /translation="MNQFVVSARKYRPSTFDTVVGQQHITHTLQNALKNNHLTQAYLF
                     CGPRGVGKTTCARILAKTLNCINLTADFEACNECEFCQMINSGQSFNMQELDAASNNG
                     VDEMRALVEQVRYAPQSGKYKIYIIDEVHMLSTAAFNAFLKTLEEPPPYAIFILATTE
                     KHKILPTILSRCQIFDFNRITIDDIAHQLTYIAGKEGITADAEGLHIIAQKADGAMRD
                     ALSMFDQIVSFAGNTVTYKDVLSNLNVLDYEYYFKITDLMTKGDYASILLVFDELLNE
                     GFDGHHFVTGMSGHLRDLMVCKDAATLKLLTVSSGVKERYRAQAEASDGEWLMALLDI
                     FNRCDIEYKTSKNQRLHVELTLMKACRVKKKFPELTEDFFEIVYEDLVNTSGDVVVAP
                     RPILQTQAQNLVAEPQPKPISISTTSQIPPIKIPAGVRMIPSIRNLGVKAPPSELGEA
                     AEVATGASKNKPFDIAELHKQVKIFADTKIDTGARNLYFTLCNHLPQLSGNRIVFLVD
                     NNNQQDLVNDVKVSLLGYLKDALQNDYITIDTSVSETPTHGKVAYTGEEKFKEMESKN
                     PELKRLRKLFDLDI"
     gene            complement(4771..5475)
                     /gene="mtgA"
                     /locus_tag="PK736_05210"
     CDS             complement(4771..5475)
                     /gene="mtgA"
                     /locus_tag="PK736_05210"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HRC32822.1"
                     /translation="MKNFWIKAWKILKRIVLWFFALSIFTVIIYKWINPPFTPLMIMR
                     VIEQAFDQKRDVKMDKDWISIDEMASCVPVAAVAAEDQQFEEHFGFDLDAIEKALEYN
                     KTHKGKTVRGASTISQQVAKNVFLFPSRSYLRKGFEIYFTFLIEVFWSKKRILEMYLN
                     IAEMGEGIYGIEAASQYYYKKSASKLTSREVASIIAILPNPRVWSPTNPNTRVARKIR
                     KVLLYMKYIDLKEVME"
     gene            complement(5551..6303)
                     /locus_tag="PK736_05215"
     CDS             complement(5551..6303)
                     /locus_tag="PK736_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRC32823.1"
                     /translation="MPRFKIILIVCLLFTTSGCNETPGINNQKQTVSNIKTDTIPVQV
                     SQKENGYEQIAKAIGLQEVTQKQDGIVVKMMYATTQNFTQTNLYGDFNKAYLLPMAAA
                     KLKTASKILQQKFPKSTITIYDAARPVVVQQQLWDLAKIEPSKKHLYLSRPEQNSLHN
                     YGAAVDVAILDSTGQMLDFGSPYDFFGEAAQPQLEPKLLKQGVLNLKQIANRKLLRSV
                     MVQAGFTPIKTEWWHFNACTRAYAKQHYKLFQ"
     gene            complement(6353..7255)
                     /locus_tag="PK736_05220"
     CDS             complement(6353..7255)
                     /locus_tag="PK736_05220"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRC32824.1"
                     /translation="MIKAIVKPVYNAMHDLLYATSGINPFFKHTHNNKPVVKGFVGDD
                     HLADPTLDDVLEKQLLHANIPVSHFHININGYKDYLQKANYPSDYYGGQPGQNNFTEK
                     TLEHFVGTCFIPFTRDMVFMDVAACTSPFSSIAQKVFGVQKVYQQDLIYPKGINGNKI
                     GGYAHETTLPSNSVDAITLHCSLEHFEGDTDKLFFAEVNRILKTGGKAVILPFYFAHT
                     YTIHIDPAFNLLRNHHPIIDAEADLHYCNWYQYHSRHYDVMQVQKRIASQMPDCSIKL
                     FRVDNFREVWDKSYLRWILEITKN"
     gene            complement(7276..>8395)
                     /locus_tag="PK736_05225"
     CDS             complement(7276..>8395)
                     /locus_tag="PK736_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HRC32825.1"
                     /translation="LGGGAPIYEREYTEPKYMSKINEYSMDKIALPDDIKKLAKYMLA
                     LPNIASKQWVYTQYDSMVGTKNMSTNIHTDAAIINLKDSKKALALTVDCNSRYVFAHA
                     ETGAAIAVAEAARNITCSGGEPSAITNCLNFGNPYNPEVYWQFVHAIKGMGAACEKFK
                     TPVTGGNVSFYNQSEIDGNIIPVYPTPTIGMLGVLKDKRNLMTLNFKEKGDKIYLIGA
                     SHNDISSSQYLANYHKVEFSPCPYFNLDEEYDLHQLIKKFIKEKCINSAHDVSEGGLF
                     VTLMESAMAGNMGFSVNTDADIRKDAFLFGESQGRIVVTINDEMESAFLEMAIESNIE
                     FLHLGEVILNEIRVDGEYFFNTNEARAIYDTALELRLN"
BASE COUNT         2577 a         1671 c         1594 g         2553 t
ORIGIN      
        1 cttgcgacca tatgttttta acaaaatcgt gcatcgaaac caaagacacc attaagtggg
       61 aagatggtaa cgaataccca ttggtaaagc ttgaaatttc gaacatgtcg catccatttt
      121 acacgggcaa aatgaagttg attgacaccg caggccgtgt agataaattc aacacccgct
      181 accgcaaggc tgccgaaaaa gccacagaac aagaataatt tattttatta aatatacaat
      241 taaaagtcct gcattgcagg gctttttttg tttagtcaca ttagtatgaa ttacattttg
      301 tttgataata gcaggcagca acttatgcct tttaccctta gccgcccggt ttgcgaaata
      361 agggttggca ttcttaccat aaaacaaaaa tgggaattgt atctcaatgc ctcatgcagc
      421 tatgagtgtg aattgtattt acaagcaaaa tatccaaagc ttaccgcatc cgataatgta
      481 tatattaatg ccactgtttt tcctgatgca gatttagtag ctgcaataaa gaatcttaaa
      541 attggcgaag cattgatatg ggaaggaaat attttggcat tgcgtacttc ggatacaata
      601 tcaaacacta ccagcatact cacaaccgaa tataaaaaac ccgtaagcag catcagccac
      661 atatggcata tatttggaat gtgtgatgaa caattacgcg ctgattttac gttgcttacc
      721 gctggtcgaa attctttaca attaaacagc agcaatagat tagttggcga tacggatgat
      781 ttatttattg aagaaggcgc caccgttaat tgcgcaataa ttaatacaca aaccggccct
      841 gtatatattg ctaaagaggc cgaagtaatg gaaggtgcca tgatacgcgg accatttgct
      901 ttgggcgaac atgctacatt gaaaatgggc gcaaaaattt atggtgctac ttcaattggg
      961 cctcattgca aagtgggtgg cgaggtgagc aacagcgtat tttttggatt ttcgaacaag
     1021 gcgcacgatg gttttgtggg caatgctgtt attggcgagt ggtgcaacct gggtgccgat
     1081 agcaataatt cgaacctgaa aaataattat ggcaatgtaa aagtgtatga ttacgcttcg
     1141 caaaatatgg ttgataccgg cttgcaattt tgcggtttgt ttatggccga ccatgcaaag
     1201 tgcgccatca atacacaatt caacacgggt accgtggtag gttttgcttc caatgttttt
     1261 gggggcgatt ttccaccaaa gcatattccc gattttagtt ggggcggtgc aaaaggtttc
     1321 gaaaaattcg atttgcaaaa agccattgcc ctggcaaaaa atgtgtatgc ccgcaggtcg
     1381 atgactttga ccgatgccga tattgccatt ttcgaagcta ttaatgaaat ggccaaaaac
     1441 accaagggtt aaatatatat tccaaccaat gcgcatcgat attataaccg tattacccga
     1501 attgctcgac agtccatttg cacacagcat tctaaagcgt ggccagcaaa aaggcatcat
     1561 cgaagtaaat gtaattccgt tgagagagtt tgccaccaac aaatataaat cggttgacga
     1621 ttatgctttt ggcggtgcag caggtatggt aatgatgtgc gaacctattg ccaattgtat
     1681 agaccattta aaattgcagc ggaactatga tgaagtaatt tatctcactc ctgatggcga
     1741 attatttagt caagctatgg ccaatcaact ttcactaaaa ggcaatttga ttatgctttg
     1801 cgggcattac aaaggggtag atgaacgcat tcgtcaacat tatatcacca aagaaatttc
     1861 gataggcgat tatgttttat cgggtggcga gcttgctgct gctgtagtga gcgatgccat
     1921 tttgcgcctg gttcccgggg ttatgaatga cgaaacttcg gcccttaccg attcgtttca
     1981 ggatggatta ctttcgccac ctgtgtatac ccgtccggca gaattcagag gcattcgtgt
     2041 gcctgaaata cttttgagtg gacacgaaaa aaacattaac gaatggaagc atcaacaatc
     2101 gctggaacgc actgcccatc gcaggcccga cctgtttgcc aagtaccatt catagcgtgg
     2161 aaaaaataaa taatttaatt gtcgcttaca ttcaaaaaaa ttctacattt gcactccgtt
     2221 ttaaaaaaac taaataaatt gcgatatgaa cataattaaa agcgtaaacg aacaaacgat
     2281 agagaaacgc gattttcctg aattcaaagc aggagatacc attacagtaa cctacaaaat
     2341 tattgagggt acaaaagaaa gagagcagtt gtttcaagga gttgttattc aacgtaaagg
     2401 agcaggtttt acacaaacat ttaccgttcg caaaatgtcg cacagtgtag gtgtagaaag
     2461 aatatttcca ttaaactcac caaaaattgc caaaatagaa gttaacaaac gtggtgtagt
     2521 tcgcagagcc aagttattct acttgcgcaa cttgtcgggc aaggcagcac gtatcgaaga
     2581 aaagaagatg taataaattc acctttgttc attttcatat aaaaggcctc ctgcaccgga
     2641 ggctttttaa ttttagtaat tttataccga aactacttag gatttcgaaa tgcataaacc
     2701 taagtgttcg gcattacggc aagtacgttt cgttttattc taaaacaaaa ctactttccg
     2761 tatattttca attatattcg cagcatattt ctcctctcct atgaatcagt ttgttgtttc
     2821 tgcacgcaag taccggccat ctacattcga tactgtagtt ggccaacaac atattaccca
     2881 taccctacaa aacgcattaa aaaacaacca ccttacacag gcatatttgt tttgtgggcc
     2941 tcgtggtgtg ggcaaaacaa cctgtgcacg tatactcgct aaaaccctca attgcataaa
     3001 tcttactgcc gattttgaag cttgcaatga gtgcgaattt tgtcaaatga taaattcggg
     3061 gcagtcgttc aatatgcagg aacttgatgc cgcatccaac aatggagtgg acgaaatgcg
     3121 tgcccttgta gagcaagtgc gttatgcacc gcaatcgggc aagtacaaaa tttatattat
     3181 tgacgaggtg cacatgttat ccacagcagc cttcaatgct tttttgaaaa cactggaaga
     3241 gccgccacca tacgctattt ttattcttgc cacaaccgaa aagcataaaa ttcttcccac
     3301 catattatca cgttgtcaga tattcgattt taatagaata acgatagatg atattgctca
     3361 tcaattgact tacattgccg gtaaagaagg cataactgcc gatgccgaag gattgcatat
     3421 aattgcacaa aaggccgatg gcgccatgcg cgatgcctta tcgatgttcg accaaattgt
     3481 gagttttgcc ggtaatactg ttacatacaa agatgtgctg agcaacctca atgtgctcga
     3541 ttacgaatac tatttcaaaa tcaccgacct tatgaccaag ggcgattacg cctccatttt
     3601 gctggtgttt gacgaattgc tcaacgaagg tttcgatggg catcattttg taacaggcat
     3661 gagtggccac ctgcgcgatt tgatggtttg taaagatgca gccacactaa aactgctcac
     3721 ggtaagttcc ggagtaaaag agcgatacag agcacaagcc gaagccagtg atggcgaatg
     3781 gctgatggca ttgctcgata tttttaaccg ttgtgatatt gaatacaaaa cctcgaaaaa
     3841 ccaacgcctc catgttgaac tgacgctgat gaaagcttgt cgggtaaaaa aaaaatttcc
     3901 tgaattaacc gaagactttt tcgaaatagt ttacgaagac cttgtaaaca caagtggaga
     3961 tgtagttgta gcaccccgac caattttaca aacgcaagcg caaaacctag ttgccgagcc
     4021 gcagccaaaa ccaataagta tttcaacaac gtcacaaatt ccaccgataa aaattcctgc
     4081 cggtgtgcga atgattccat ctattcgtaa tctgggtgta aaagcaccac catccgaatt
     4141 gggtgaggct gccgaagttg caacaggagc atccaaaaac aaaccatttg atattgccga
     4201 attgcataag caggtaaaaa tatttgccga caccaagatt gatacaggag cacgtaattt
     4261 atatttcact ctttgcaatc atttgcctca attgagtgga aatagaattg tatttttagt
     4321 cgacaataac aaccagcaag accttgtaaa cgatgtgaaa gtaagtcttt tgggttacct
     4381 taaagatgca ttgcaaaacg attatattac tatcgataca tcggtaagcg agacaccaac
     4441 acatggtaaa gtggcatata caggagaaga aaaatttaaa gaaatggaat cgaaaaatcc
     4501 cgaattgaaa agactaagaa aattgttcga cctcgatatt tagcactgga acgctgattt
     4561 ttatgatggt gatgattttt taagatttaa cagtgctctt caaaatctta caccatacta
     4621 ttattttttg gaaatttttg aatcgcgcct tactgaacta aaacataaat tcgtttttta
     4681 catttcaata tttaccgact aaatatttaa taaaatgaaa ctctttattt cattcttgat
     4741 ttcatcacat aaaaaataaa aatgccccat ctactccatc acttctttta agtcgatgta
     4801 tttcatgtac aacaatactt ttctaatttt tcgagcaaca cgtgtattgg gatttgtagg
     4861 tgaccacaca cgaggattgg gaagtatagc aataatggaa gccacttcgc gcgatgtaag
     4921 tttcgatgca ctcttcttgt aatagtattg cgatgctgct tcaataccgt aaatgccttc
     4981 gcccatttcg gcaatgttga gatacatttc aagtatgcgt tttttgctcc aaaacacttc
     5041 aatcaaaaaa gtaaaataaa tttcgaagcc tttgcgtaag tatgaacgcg aaggaaaaag
     5101 aaaaacattt tttgccacct gttgcgatat agtgcttgcc ccgcgtaccg ttttcccttt
     5161 atgtgttttg ttatattcaa gtgctttttc aatcgcatcg aggtcgaaac caaaatgttc
     5221 ttcgaattgt tggtcttctg ctgcaaccgc tgccaccggc acacagcttg ccatttcatc
     5281 tatcgatatc caatctttat ccatcttcac atcgcgcttt tggtcgaagg cttgttctat
     5341 tacacgcata atcatgagtg gtgtaaaagg cggattaatc catttgtaaa taataacagt
     5401 aaaaatactt agggcaaaaa accacagcac tatccgcttc aatattttcc atgctttaat
     5461 ccaaaaattt ttcatctaca gcaaaataat tattctcata cattcatatt cgtttgcaat
     5521 gataaaataa aaactgcacg acccaccact ctattgaaac aacttataat gttgctttgc
     5581 ataagcgcgg gtacatgcgt taaagtgcca ccattcagtt tttatgggcg tgaatcctgc
     5641 ctgcaccatt acacttctta gtaacttccg gttggcaatt tgctttagat ttaaaacacc
     5701 ttgttttaat agcttaggtt cgagttgagg ctgtgcggct tcaccaaaga aatcgtaagg
     5761 cgaaccgaag tcgagcattt gaccggtgct atccagtatt gcaacatcca ctgcggcccc
     5821 atagttgtgc agcgagtttt gctcaggacg cgataagtat aaatgttttt tcgatggttc
     5881 aattttggct aagtcccata attgttgttg cactactaca ggccgtgctg catcgtatat
     5941 ggttatagtt gattttggga atttctgttg caatatttta ctggcggttt tgagttttgc
     6001 agctgccatt ggcaataaat atgctttatt aaaatcgcca tacaaatttg tttgtgtaaa
     6061 attctgcgta gtggcataca tcatttttac cacaatgcca tcttgctttt gtgttacttc
     6121 ttgcaatcct attgctttgg ctatctgctc atatccattt tccttttggc taacttgaac
     6181 tggtattgta tccgttttta tatttgatac cgtctgcttt tgattattta taccaggggt
     6241 ttcgttacag ccacttgtag taaataaaag acaaacgatt aaaataattt taaaccgtgg
     6301 catagtggat caattaaaat ttattgaaag tgctaacatt gatcaattac aattaatttt
     6361 ttgtaatctc gagtatccac cgcaaataac ttttgtccca cacttctcta aaattatcaa
     6421 cgcgaaataa tttgattgag cagtcaggca tttggcttgc gattcgtttt tgaacttgca
     6481 ttacatcata atgcctgctg tgatattgat accaattgca ataatgcaaa tcggcttcag
     6541 catctattat gggatgatgg tttcgcaata aattaaatgc cggatcaata tgaatggtgt
     6601 aagtgtgtgc aaagtaaaaa ggaagtataa cagccttgcc tccagttttt aaaattctgt
     6661 tcacttcggc aaaaaataat ttatccgtat cgccttcgaa atgctctagc gagcaatgca
     6721 atgtgatagc atcaaccgag tttgacggca atgttgtttc gtgtgcatac ccacctattt
     6781 tattaccatt aattcctttg ggataaatta aatcttgttg atatactttt tgcacaccaa
     6841 aaactttttg cgcaatagaa ctaaatggcg atgtacatgc agctacatcc ataaaaacca
     6901 tatcgcgggt aaaaggaata aagcaagtgc ctacaaaatg ctccaatgtt ttttcggtaa
     6961 aattattttg ccccggttgt ccaccataat aatcggaagg ataatttgcc ttttgtaaat
     7021 aatctttgta tccgtttata ttaatgtgaa aatgacttac aggaatattt gcgtgtaaca
     7081 attgtttttc aagcacatcg tcaagggttg ggtctgctaa atgatcgtca cccacaaaac
     7141 cttttacaac cggcttatta ttatgggtgt gtttgaaaaa aggattgata ccactggtag
     7201 cataaagcaa atcgtgcatt gcattataaa ccggctttac aattgctttg atcatttgta
     7261 ctatcggtta aaaaattaat tcaaccgcaa ctcgagtgca gtgtcgtaaa tggcacgggc
     7321 ttcattggtg ttgaaaaaat attctccatc aacacgtatc tcattcaata ttacttcgcc
     7381 cagatgcaaa aattcgatat tcgattcgat ggccatttct aaaaatgcac tttccatttc
     7441 gtcatttatt gttaccacta tgcggccctg actttcgcca aataaaaatg catctttacg
     7501 aatatctgca tcggtattta cagaaaaacc catgtttcct gccattgccg attccataag
     7561 ggttacaaac aatccgcctt cgcttacatc gtgtgcacta tttatacatt tctctttaat
     7621 aaactttttt atcagttgat gtaaatcata ctcctcatca agattgaaat atgggcaagg
     7681 agaaaattca accttgtgat aatttgccaa atattgcgat gacgatatat cgttgtgcga
     7741 tgcacctatc aaataaattt tatcgccttt ttccttgaag tttagcgtca tcaaattgcg
     7801 tttatctttt agcacaccaa gcataccaat tgttggtgtt ggataaacag gaatgatatt
     7861 gccatcgatt tcactttgat tataaaaact aacattgcct ccggtaacag gagttttaaa
     7921 tttttcgcat gccgcaccca ttccttttat ggcatgtaca aattgccaat acacttcggg
     7981 gttatacgga tttccaaaat tcaaacaatt ggtaatggcc gaaggttcgc caccactaca
     8041 agttatatta cgtgcggctt cggcaacagc aatggctgcc cctgtttcgg catgggcaaa
     8101 cacataacgc gaattgcaat ctaccgtaag tgcaagcgct tttttagaat ctttcaaatt
     8161 aataatggcc gcatcggtat gaatattcgt actcatattt tttgttccaa ccatactatc
     8221 gtactgcgta tacacccatt gtttacttgc tatgtttggc aatgccaaca tatattttgc
     8281 aagtttttta atatcatcgg gtaaggcaat tttatccata ctgtactcat ttattttgga
     8341 catgtatttt ggctcagtgt attcacgctc atagattggt gctccaccgc caagt
//
LOCUS       DAORQA010000065         8283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2265_length_8283_cov_10.033179, whole genome shotgun sequence.
ACCESSION   DAORQA010000065 DAORQA010000000
VERSION     DAORQA010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2265_length_8283_cov_10.033179"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..806)
                     /gene="lptB"
                     /locus_tag="PK736_05230"
     CDS             complement(81..806)
                     /gene="lptB"
                     /locus_tag="PK736_05230"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRC32826.1"
                     /translation="MILKSTHLVKKYKSRTVVNDVSIQLQQGEIVGLLGPNGAGKTTT
                     FYMTVGLIKPNAGNVFLGDKDITNEPMYKRARMGIGYLAQEASVFRTLSVEDNIKAVL
                     EMTALSKTEQYDKCEELLDEFGLNKVRKSPGNVLSGGERRRTEIARALAVSPKFILLD
                     EPFAGVDPIAVEDIQQIVVKLRTKNIGILITDHNVHETLGITDRAYLLFEGKILKHGT
                     PQELADDETVRRVYLGQNFELRQ"
     gene            complement(1036..6087)
                     /locus_tag="PK736_05235"
     CDS             complement(1036..6087)
                     /locus_tag="PK736_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRC32827.1"
                     /translation="MRKITFILFTFFIFSVNAYAQPYGNEWIKYTFGNPFSDQEYFKL
                     SIAKDGVYRLTYSDLLAAGFPVTEDPRKFQMFAYGKEQYIQVAGEADGTFDVTDFIQF
                     LGYRNTGAIDSALYAQPSYQKNTKRSLISDTVSYFITVSQNSINNKRVIDEKDVNFSN
                     YGSSPYFMRNLFNYNVPNGNYYYDSYHDTFGSALSAYMDGEGFAIDYLGAGAQNSSTT
                     WAALSTPNATNVANTNINTFLGAETSYDHPYKVTVDGVDVVNSTFNGPFYKEDNVSVS
                     HSNGTMNFIVTALANPQGAAEPNGFFVRYYDIDYPHTYSFNGEALNLYSMQIAPASNT
                     AKSTLNITNLNLAQPILYTYNGDTVKNVSLSQTGNVWQAVVPTYYQPVKGMLVDASAI
                     ITNSGNVLKLTKVNYKGAPAGKFNNFANDGTKNYLIVTHAKFWNQATNYKNYRENNPV
                     KNFNVLLADIHELYDQFAYGVEFHSLSIKGFAKFTLNNFAQKPEDIFIIGKGIMPQFS
                     LDDHTGIYVPTFGSPPSDLMFTVGIQANDTVQRPAIAVGRFPALEPYEVQDYLDKMIT
                     YEAATKAEWMKKVMHFAGGSNANESQGIQQLMNGFANIIEDTLMGAYVRTLSKTANTP
                     IQIDLSANLQNIIDSGLTLMTFFAHAASSSFDISTDVPSSWQNKDRYPLIIANSCYVG
                     NMHKLGRYAAEDFVMQKQKGAIGFVGQASLGYVFNLWPYTTNLYKNFSWFNYGKSIGE
                     QLLATIDSMQINYNDYGLDKFYAQAVALDMNLLGDPALHLNSPDKPDLVAATSDINIL
                     PKTLSTADDSLTIKITLRNIGRAVTTPFIVKISRTFANMNTVDTLLQLNKLAYTDTVT
                     FRMAVDRAQGAGLNTFSFELDLNDQIQNECSEANNSAIQLFIKSNDINALFPTKYAII
                     PDSNVTLVAATANVLAPPTVYVFQIDTNDTFNSPFMIEHKVTAPGGIINWALPFMADQ
                     DRVYYWRVSRDSIAGDLEHPYWDETSFVFKSGKSGWSQGHIYQFKDDGYNNIVWNRDN
                     QNWNFIKTKSVIRANNNINNNGLEMNYYINGIVQAAASCFPQGVNAGINVAVIDDITL
                     APWNNREPGRNYGNWNNDNTKCNANRPDNFFAFPSSTLVPNYLDSLNDMLQNDIPVGK
                     YLLMYSIDKNWYNAIPQNLKNTITALGSTNINFLNDKNCWIMFTRIGDPSATHEVIGD
                     SLNPIVTLFDSRGGNWFQGYMVSEKIGPGVKWSSLHWETSQIEPLDSVTLDVVGYKQN
                     GTSAVLIDDLAPTVNDIFNLDIYADTSYKYLELRSYMQDDSIVPDPVYMDKWQIFYQP
                     LPEAMVDPSLYLNISKTKLNEADTVTVTTAIKNIGTQPMDSMLVSYYLYDAKNKKYDY
                     PSKMYKPLNPGDTLHCSVTLPTAGLGGGNTLWIEANPNNNQPEQYHFNNYAQVSLNVD
                     VDITNPILDVTFDGIHILNEDIVSARPRIQVSLKDENPVLVLDDTSDFYITLDEATVF
                     QPRRIYFEPIEGTTTSSDLLTWTKGKLPDNKFKISISPVLENGLYKLVVQGKDKSNNS
                     SAHQSYNITFEVENKPTITHVMNYPNPFSTSTRFVFTLTGSEIPEYFKIQVMTVTGKV
                     IREINREELGPLHIGRNITSYAWDGTDEFGDRLANGVYLYRVITKLNGEDIEARETDA
                     DHFFVNGWGKMYLLR"
     gene            complement(6184..7281)
                     /locus_tag="PK736_05240"
     CDS             complement(6184..7281)
                     /locus_tag="PK736_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRC32828.1"
                     /translation="MSKQKIFLAAIFFFGSALTSFSQAVKYSNEFLSIGVSARAQGLG
                     GSVVAQVNDVTAGYWNPAGLVLKQGDLQIGLQHAELFAGILKYDYGAICAPIDATRAV
                     GLSIIRLAVDDIPDTTELLDADGNVDYNRIKSFSSADYGFIFTYSKKTKKEGLRYGAN
                     AKVIHRKAGEFASAWGFGLDAGIQYQKNKWTFGVMGRDITSTFNAWKFNTDKLATVFA
                     QTGNEIPQNGVEVTLPKLIPGAAYSFKLGKKFNLITELDADLTFDGKRNVLIKSNAVS
                     VDPHFGFELGFDNLLFLRGGIKNIQKVENIDASVSTIAQPSLGLGLRIKNISLDYAVS
                     NIGQQGDIPYSHIFSLQFNIIKQKKKVADVK"
     gene            7431..>7849
                     /locus_tag="PK736_05245"
     CDS             7431..>7849
                     /locus_tag="PK736_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRC32829.1"
                     /translation="MTKHIPNTLTCGNLVCGCLGILAATHHQLQLAVYFILIAAVLDF
                     FDGFLARKLNAYSIIGKDLDSLADAVTFGVLPGFIVFALMNSNSIFTINIFTESVPMS
                     FLAFLIPVFSILRLAKFNNDPRQTDSFIGLPTPACTL"
     assembly_gap    7850..7869
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7870..8179
                     /locus_tag="PK736_05250"
     CDS             <7870..8179
                     /locus_tag="PK736_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32830.1"
                     /translation="DAHTNFSFGLFQSSQAVISLSKSEPNIYAFVALIAILCFLLICE
                     LPLFALKFKSFALKENLLRYIFLLCGLILLIKFNFAAFAYIIPLYILMSVFTQKMSKV
                     "
     gene            8191..>8283
                     /locus_tag="PK736_05255"
     CDS             8191..>8283
                     /locus_tag="PK736_05255"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRC32831.1"
                     /translation="MKFKASIDIMPLKALLDPQGKAVSATMKNIG"
BASE COUNT         2429 a         1621 c         1532 g         2681 t
ORIGIN      
        1 gtattgaata tcatgataca agttcagaat gattttaatt aaacacaact ttgaagaaat
       61 tgttacgcat gcaaaaacaa ctattgtctg agttcgaagt tttgtccgag gtatactctg
      121 cgcacggttt cgtcatcggc aagttcttgc ggtgtaccat gcttcaaaat ttttccttcg
      181 aataataaat atgccctatc ggtaataccc aaagtctcgt gcacgttatg atcagtaatg
      241 agtataccga tatttttggt tcgcagcttg acaactattt gttgaatatc ttcaacagca
      301 atagggtcga ctcctgcaaa aggctcatcg agtaaaataa attttggact tacagcaagt
      361 gcacgggcta tttcggtacg ccttcgctcg ccacccgaca atacattacc ggggctttta
      421 cgcactttgt tcaatccgaa ttcgtcaagc aactcttcgc atttatcata ctgttctgtt
      481 ttgctcaggg cagtcatttc cagcactgct ttaatattat cttctacact aagtgtgcga
      541 aaaacacttg cctcttgagc aaggtaacct atgcccattc gtgcacgctt gtacatcggt
      601 tcgtttgtta tatccttatc gccaaggaaa acattgcctg cattgggttt tatcaaaccc
      661 acggtcatat aaaaagtagt ggttttgccg gcaccgttgg ggcctaatag tccaacaatt
      721 tcaccctgct gcaattgaat actaacatcg ttcaccacag tgcggctttt atatttcttt
      781 actaaatgag tcgattttaa aatcatattc aattttttac tgcccacaac tataagtgta
      841 tacgaagcga gtacaatgca gctttttgga cagcgcaaaa atgcacaaat aaattgtaaa
      901 taggaatgag cctaagcgat ataatagcga aataacgaaa gtttacaaaa aatattcaat
      961 tgtagttaat cgtcattaac aaaagaaagc cgcttttttg aagaactgca aactttgggt
     1021 aaaacttaaa agtaattatc gcagcaaata catcttaccc catccgttaa caaagaaatg
     1081 gtcggcatcg gtttcgcgtg cttctatatc ctcaccattc agttttgtaa tcactctata
     1141 aaggtaaaca ccgttggcaa gcctgtcgcc aaattcatcg gtgccatccc aggcgtacga
     1201 tgttatgttt cggccaatat gcagcggccc tagttcttcg cggtttattt cgcgtatcac
     1261 tttaccggtt actgtcatta cttgtatttt gaagtactcc ggtatttcgg aaccggtgag
     1321 ggtaaataca aatcgtgtgc ttgtagagaa cggattggga taattcatca catgagtaat
     1381 ggtaggtttg ttctccacct cgaaagtaat attgtatgat tggtgtgccg aagagttatt
     1441 gcttttatcc tttccctgca ccacaagttt gtataatcca ttttctaaaa caggacttat
     1501 gcttatttta aatttattat cgggcaactt gccttttgtc catgtaagta aatcgctact
     1561 ggtggttgtg ccttctatgg gttcaaagta aatacgtcta ggctgaaaca ccgttgcttc
     1621 atcgagggtg atgtaaaaat cgctggtatc atctaaaacc aacacagggt tttcatcctt
     1681 taacgatacc tgtatacgtg gccttgccga aacaatatct tcattgagga tgtgaatacc
     1741 atcaaatgtt acatcgagta taggattggt aatatctaca tctacattca aacttacctg
     1801 agcataatta ttaaaatggt attgctcggg ttggttgtta ttgggattgg cttctatcca
     1861 aagtgtattg ccaccaccaa gacctgctgt gggcaatgtt acactgcaat gcaatgtgtc
     1921 gccaggattg aggggcttgt acatcttgct cggataatcg tactttttat ttttagcatc
     1981 gtacaaataa tatgatacga gcatgctgtc cataggctga gtgccgatgt ttttaattgc
     2041 cgtggtaacg gtaactgtat ccgcttcgtt caattttgtt ttcgatatgt ttaagtataa
     2101 agatggatcg accattgctt cgggtaaagg ctgataaaat atttgccact tatccatgta
     2161 aaccggatcg ggaacaatgc tgtcgtcttg catatatgat cgcaactcga ggtatttata
     2221 agaagtatcg gcatatatat cgagattaaa aatatcgttt acggttggcg ctaaatcatc
     2281 tatcaacact gcgcttgttc cattttgttt atagccaact acatctaatg taacagaatc
     2341 taaaggttca atttgcgaag tttcccaatg caaagagctc cactttacac cggggccaat
     2401 tttttcagag accatgtatc cctgaaacca gttaccgcca cggctgtcaa ataatgtaac
     2461 tattggattg agcgaatcgc caatcacctc atgagttgcc gatggatcgc caattcttgt
     2521 aaacattatc cagcagtttt tgtcgttcaa aaaattaatg tttgtactac caagtgctgt
     2581 gattgtattt ttcaaatttt ggggaatggc attgtaccag tttttatcaa tgctatacat
     2641 caacaagtac ttacccacag gaatatcgtt ttgtaacata tcgttcaaac tatcaagata
     2701 atttggaaca agcgtagaag aaggaaaagc aaaaaaattg tcgggacgat tggcgttgca
     2761 tttggtgttg tcattattcc agtttccata atttcgtccc ggctcgcggt tgttccaggg
     2821 tgcaagtgta atgtcatcta tcacagctac gttaatgccg gcattcaccc cttgcggaaa
     2881 acaacttgct gcagcctgca ctataccatt gatgtaataa ttcatttcga ggccattatt
     2941 gtttatatta ttattggcac gaataacact tttggtttta ataaaattcc aattttgatt
     3001 atcgcggttc caaacaatgt tattatagcc atcgtcttta aactgataaa tgtgcccctg
     3061 cgaccacccg gattttcctg atttaaaaac gaatgaagtt tcatcccaat aagggtgttc
     3121 taaatcaccg gcaattgaat cgcgcgatac acgccaataa tacactctat cttgatcagc
     3181 catgaaaggt aatgcccagt tgataatgcc gcctggtgca gtcaccttat gttcaatcat
     3241 aaaaggtgaa ttaaatgtat cgttggtatc aatttgaaaa acataaaccg taggcggagc
     3301 caatacatta gcagtagctg ctaccaaagt aacattacta tcgggaataa tggcatactt
     3361 agttgggaac aatgcattta tatcattcga ttttataaat aactgtatgg cactattgtt
     3421 ggcttcacta cattcatttt gtatttgatc atttaagtca agttcgaaac taaatgtatt
     3481 taaaccggca ccctgagcac ggtctacagc catgcggaat gtaacagtat ctgtatacgc
     3541 taatttgttt aattgcagaa gtgtgtcaac agtattcata ttggcaaacg tgcgtgatat
     3601 ttttactatg aaaggtgtag ttacagcacg gccaatatta cgtaaggtaa tttttattgt
     3661 gagcgaatca tcggcagtag aaagtgtttt tggtaatatg ttgatatcgc tggttgcagc
     3721 tactaagtct ggtttatcgg gagaatttaa atgcagggca gggtcgccca gaagattcat
     3781 gtcgagtgca actgcttgag cataaaattt atcgaggcca taatcattat agttgatttg
     3841 catactatcg atagtagcta ataattgttc gccaatagat ttgccatagt taaaccacga
     3901 aaaatttttg tacaagtttg tggtgtaagg ccacaaattg aaaacgtaac ctaacgatgc
     3961 ctgaccaaca aatccaatag cacctttttg tttttgcatc acaaaatctt cggcagcata
     4021 acgcccaagc ttatgcatgt ttccaacata gcacgagttg gcaataataa gaggatagcg
     4081 gtctttgttt tgccacgatg aaggaacatc ggtgctaatg tcgaaagaag aacttgctgc
     4141 atgcgcaaaa aatgtcatca atgtaagtcc gctatctatt atgttttgca ggttagcact
     4201 taagtctatt tgtatgggag tgtttgcagt tttgctaagt gtgcgcacat atgcacccat
     4261 caatgtgtct tcaattatgt tagcaaaacc attcatcaat tgttgaattc cctggctttc
     4321 gtttgcattg gagcccccgg caaagtgcat cacttttttc atccactctg cttttgttgc
     4381 tgcttcgtag gtaatcattt tgtcaaggta atcctgcacc tcgtaaggtt ctaatgcagg
     4441 aaatcgtcct acagcaatgg caggacgttg tacggtatcg ttggcttgaa ttcccacggt
     4501 gaacatcaaa tcgctgggag gagaaccaaa agttggcaca taaatacctg tatgatcatc
     4561 gagcgaaaac tgtggcatta tccctttacc aatgataaaa atatcttcag gtttttgtgc
     4621 aaaattatta agagtaaatt ttgcaaagcc tttaatacta agcgagtgaa attctactcc
     4681 atacgcaaat tgatcgtaca actcgtgtat atcggccaac aaaacattaa agttttttac
     4741 cggattgttt tcgcgatagt ttttatagtt tgtggcttgg ttccaaaatt tagcatgggt
     4801 tactatcaaa taattttttg tcccatcgtt tgcaaaatta ttgaactttc cggccggagc
     4861 tcctttgtaa tttacttttg taagtttcag cacatttcct gagttggtaa taattgcaga
     4921 agcatctaca agcattcctt ttacaggttg gtagtaggtt ggtaccaccg cttgccaaac
     4981 attgcccgtt tgcgaaagag aaacattttt tacagtatcg ccattgtacg tatacagtat
     5041 aggttgtgct aagttgaggt tggtaatatt tagcgttgat ttggcagtgt tagaagccgg
     5101 tgcaatttgc atcgaataca agtttagtgc ttccccatta aacgaatatg tgtgtggata
     5161 atcgatatca taatatctta caaaaaaacc atttggctca gcagcgccct gagggttggc
     5221 aagggcagta actataaaat tcatagtgcc gtttgaatga gaaacgctca cattatcctc
     5281 tttgtaaaat ggtccgttaa aggtgctatt aacaacatct actccatcaa cggttacttt
     5341 atatggatga tcgtaacttg tttcggcacc taaaaaagta ttaatgtttg tatttgccac
     5401 attggtagca ttgggtgtgc ttaatgcagc ccaagttgta gagctatttt gagcaccggc
     5461 tcctaaataa tctattgcaa aaccttcacc atccatatat gcactcaggg ccgagccaaa
     5521 tgtgtcgtgg taactatcat agtaataatt tccatttggc acattgtagt tgaacaggtt
     5581 gcgcataaaa taaggtgagc ttccatagtt actaaaattt acatcctttt cgtctattac
     5641 acgcttattg tttatcgagt tttgacttac tgtaatgaaa tacgaaactg tatcgcttat
     5701 caagctgcgt ttagtgtttt tttgatatga tggttgggca tacaatgcag agtcaatggc
     5761 gcccgtatta cgatatccta aaaactgaat aaaatctgtc acatcaaaag ttccgtctgc
     5821 ttcacctgct acttgtatat attgttcttt gccataggca aacatctgaa acttgcgcgg
     5881 gtcttcggta acaggaaacc cggctgcaag caaatcggaa taagttagtc ggtatacacc
     5941 atcttttgca atacttaatt taaaatattc ctgatccgaa aacgggttgc caaaagtgta
     6001 ttttatccat tcgttgccat aaggttgtgc atatgcatta accgaaaaaa taaaaaacgt
     6061 aaacaatata aatgtaattt ttctcattcg tgcataattt aaaattgttt tttcctggat
     6121 cgtaattttt tatatttatt aatctttaaa gaaattaatt agcaaaataa aattgaaaat
     6181 ggattactta acatctgcca cttttttctt ttgtttgata atattaaatt gcaaagaaaa
     6241 aatatgtgag tatggaatat cgccttgttg cccaatatta cttaccgcat aatcgagtga
     6301 aatgtttttt atacgcaagc caagtcccaa tgatggttgc gcaatagtgg acaccgaggc
     6361 atcaatattt tctaccttct gaatattttt aatccctccg cgcaagaaca ataaattgtc
     6421 gaaaccaagt tcgaaaccaa aatgaggatc cacacttaca gcattcgatt ttataagcac
     6481 attgcgctta ccatcgaaag ttaaatccgc atcgagttcg gttatcaaat taaatttctt
     6541 tcccaacttg aatgagtatg ctgcaccggg aatcaatttt ggtaatgtta cttccactcc
     6601 gttttgagga atttcattac cggtttgtgc aaacaccgtg gctaatttat cggtattaaa
     6661 tttccatgca ttaaatgtgg atgtaatatc gcgtcccatc actccaaacg tccatttatt
     6721 tttttgatat tgtattccgg catctaaacc aaaaccccat gcgctggcaa attctcccgc
     6781 tttacgatga ataacttttg cattggcacc atatcgtaac ccttcttttt ttgtcttttt
     6841 cgaataggta aatataaaac cataatcggc cgaagaaaaa gattttatgc ggttgtaatc
     6901 cacattacca tcggcatcta ataattctgt ggtatcggga atatcgtcta cagcaagtct
     6961 aataatggaa agaccaacag cacgtgtagc atctatagga gcacaaatag caccataatc
     7021 gtattttaaa atacctgcaa acaactcggc atgctgcaat ccaatttgca aatcgccttg
     7081 cttaagtaca agccctgccg gattccaata acctgcagta acatcattaa cctgcgctac
     7141 cacactaccg ccaagccctt gtgcgcgggc acttacacct atcgataaaa attcattact
     7201 atactttact gcttgactaa aagatgttag tgctgaacca aaaaagaaaa tagcggcaag
     7261 aaatattttt tgttttgaca tagtgttttt gttttgcatt tgtaaacgga aacccgcaga
     7321 taatagtatt attattcggg taaatataaa tgttatatag caataattgt gctattagtt
     7381 tgtgtaattt tgccttgttg catttttaaa taatcaaaca cgtcttgaat ataacaaaac
     7441 atattcctaa cacacttaca tgtggcaacc tggtttgcgg ttgtttaggc atacttgctg
     7501 ctacacatca tcaattacaa ttggctgttt attttattct tattgcagcc gtgctcgatt
     7561 tttttgatgg ctttttggcg cgaaaactaa acgcctatag tattattggt aaagacctcg
     7621 actcccttgc cgatgccgtc actttcgggg tgttgcccgg ctttattgtt tttgctttga
     7681 tgaacagtaa ctccattttt acgataaata tttttacaga atctgtaccg atgagttttc
     7741 tcgcatttct cattccggta ttttcaatat tgcgattagc taaattcaat aacgaccctc
     7801 gccaaaccga ttcgtttatt ggcctgccca ctcccgcatg cactttgttn nnnnnnnnnn
     7861 nnnnnnnnnc gatgcacata caaattttag cttcgggtta tttcaatcat cacaggcagt
     7921 tatctcattg tcaaaatcgg aaccaaacat ttatgctttt gttgcactta tagccatttt
     7981 atgtttctta cttatatgcg aacttccgct ttttgcgctc aagtttaagt cctttgcatt
     8041 gaaagaaaat ttattgcgtt atattttttt gttatgtgga ctcatattgc ttataaaatt
     8101 taatttcgca gccttcgctt acatcattcc actatatatt ttgatgtcgg tgtttacaca
     8161 aaaaatgtca aaagtttaaa acgaaaaaaa atgaaattca aagcctccat agatataatg
     8221 ccactaaaag cattactcga tccgcaaggc aaagcagtta gtgccactat gaaaaatatt
     8281 gga
//
LOCUS       DAORQA010000066         8134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2325_length_8134_cov_10.888229, whole genome shotgun sequence.
ACCESSION   DAORQA010000066 DAORQA010000000
VERSION     DAORQA010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2325_length_8134_cov_10.888229"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2783
                     /locus_tag="PK736_05260"
     CDS             <1..>2783
                     /locus_tag="PK736_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRC32832.1"
                     /translation="LTAGTYTVTATDANNCTTTASYTLTQSAAISISLVSTNVSCNGG
                     GNGSITANASGGSGTYNYTWSNGASTASINGLGAGIYTITVTDGNGCTMSSSATITSP
                     SALGCICNSSVTNVSCFGGSNGSVSALPLGGTAPYNYLWNNGATTQSISGLVAGTYTV
                     IITDANGCTKVGTATVTQPSQLFVFVAGQNLTCAGNNTGAATATPSGGVAPYTYLWTP
                     AGQTTQSATGLAAGNYTVKVTDANGCTATAQVTITQPPSMTLTSKRTNVSCFGGFNGT
                     FTVVVNGGTPAYSYLWSNGKTTKFIIGLGAGAYTVTVTDAAGCTKTLSGIITQPPAMV
                     ALYTVTNVNCNGGSNGAIDLTITGGTSPYTYVWSNGALTQDITGIAAGIYAVTVKDKK
                     GCIINATVVVSQPTPITLSMTPKSPKCFGGNNGQATVTASGGTVPYSYLWSNGKTTST
                     ATGLTAGTYTVTVTDSKACSATMSVTISQPPLLACTANVIQGILCNGGLGKIGVTATG
                     GTPGYSYTWNTNPITTTAIANNVVAGTYTVIVKDKNNCTTSCVVTITQPAAITCAPLT
                     STPVSCNGANDGTATVSGIAGGTSPYTLLWNSFVPQTTPTATGLSAGTYTVTITDANG
                     CTKTASVIVTGPTALTATYSSISPGCNGGVGTITVVAAGGSPAYSYTVNPGAINNTTG
                     VFGGLSAGTYSVLVTDANLCTTVISNIVITSGNTITANPLTSTNVNCNGGNNGTASIT
                     GISGGLAPYTLIWSTVPVQGTLTATGLTAGTYTVTVTDANGCIYTAAAIVTEPSTPVT
                     ATINSQINIDCNGGGLGEATVLASGGSSTNSYLDNNFAAAFPSGLVLGGTGGCGTPYT
                     LTFTSPAAIRAFIPNALGLAAPLTMDMTDPTNGSYINSLAGALVATTLNVQFDLYDAN
                     FAPTSTVHLADLI"
     assembly_gap    2784..2798
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2803..7908
                     /locus_tag="PK736_05265"
     CDS             2803..7908
                     /locus_tag="PK736_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32833.1"
                     /translation="MTVQQILDESNNALAGCGSIYTPNQLRTVLQQINHSWENGVPGN
                     GFLSCPVGPPGCNTFYSLTQEGWDATGGYTYLWSNGQTTATATGLTAGTYVVTVTDAN
                     GCTAVTQVTITQPGSLTVSAAAINALCNGGANGMVLASAQLGTPPYSFMISPMAGTQT
                     NPTADSAVFTGLAAGTYTVIVTDAGDCTAETDVTVNEPSAIASNTTHVNVSCNGGSNG
                     VATVYAIGGTPGYTYQWSNGTSVVSTNQQATGLPAGTYTVTVTDANGCINTGSTTIAQ
                     PAVLSITPTQVNVSCNGQGTGSATATVTGGTAPYNYLWNNGQQSSTATGLMAGTYTVT
                     VTDFKGCTNTQSYSITSGVNIIVNPSQTPILCNGGTSVLTVNPTGGTGTYTYSWSQAP
                     LNTTNTATVNAGVYYVTVTDSAGCSVTYCFVVAQPNAVACAISSTGVSCNGSANGSAT
                     ANVIGGTLPYSYSWNDANSQATQTAIGLGAGTYTVLVTDSNGCTSQCSITITEPQALS
                     VGTSNTNVSCQNPTGTAVAIVGGGTAPYTYLWSNGASTSAITGLLPGTYTVTVNDSKG
                     CGPVTAQATILAPVAVTCSATSTMTSCNGYHNGTATVTPGGGNGVYSVIWNTTPAQTT
                     LTATGMSAGTYSVVVLDGLGCSSTCTVVVQEPAPLVYSYNINNTCAGSNGGTSTINAM
                     GGTMYTNGNPYTYLWNNGQTTQTATGLGSGTYYVTVTDSLGCAIQAVIIMPQAQSMTC
                     NTFCTPTACGQATGTATVNVFGGTMPYTYQWSNGNTNVINTGLTAGTYTVLVTDAQGC
                     TTMCIANVGQPANIQYTLAVVNPACNGGTGSVTITNLNGGMSPYSYLWNNGSTALSVS
                     GLAGTYTVTVTDINSCSITMTATIVQPLILNVTTSAVNVTCNGYTNGSAAAVVSGGTA
                     PFNYLWNTGGNLSYMNNLAAGTYTVTVTDANGCIATAQSVIAQPLAMDTVSTSSISST
                     CGLCNGSGSLVITGGTAPYSYLWSNGATSDNATGLCAGNNFVTVTDANGCTISVCVKV
                     SNTGSFAAALTSPTYAGGYNIRCNGGLDGAVNLTVNPIGTYTYSWSNTSTNEDLFGVG
                     AGTYTVTVSNGTCTQTVSITLTEAPLLTASATGTNANCSGTNTGTATALGIGGVMPYT
                     YAWSNGQTTQTATGLMAGTYTVIIGDANGCTANATYTVTGVVNALSITETHVNPTCYG
                     NTNGSIDITVTGGSLPYTYIWTGGATTEDRTNIGAGSHTVTVTDANGCTITLCVVVTQ
                     PNAIVISMSTTNATCNGGSNGSAAAAVSGGSSPYTYLWNTGANTANINGLAAGTYTVT
                     VTDASGCTFSANKAVGAPVSMTVIVDSVKNVTANGGNNGAAYISLNGGTAPFVYSWNT
                     IPVKTTQDVTGLTAGTYQVSVTDAAGCVANATVTITQPNYICSNGTPFHTETPAAWTV
                     AGNGSNIGTYLTLHFPNAFPGSLVVGGGCLGSKTLTLTNATAVRSFLLGQPLTGGANL
                     LSSNLNNPTNATFSSALAANIVALKLAVVFDAYDGNFAPVPTVWLANLIYNNPAGPAA
                     LNGLTVNQVLFEAEKKLGGCSSSFTGAQLTTALNNINLSYLGGVAGASSTMLTCPAPP
                     KLGDVVTTIGNIVAYPNPTEGQLNIRFVTEQDGKVSVRLFDAIGRLAFNTTETAFEGV
                     NERSYDVSNLSKGVYMLNVQVNGLTETIRVVVK"
BASE COUNT         2415 a         1633 c         1812 g         2259 t
ORIGIN      
        1 cctcaccgcg ggtacgtata cagtaactgc tactgatgca aataattgta ccacaactgc
       61 aagctatact ttaactcagt cagccgcaat atccatttca cttgtaagta ccaatgtaag
      121 ttgtaacgga ggtggtaatg gaagtattac ggcaaatgca agcggaggct caggcacata
      181 caattacact tggagcaacg gagcaagtac tgcaagtatt aatggtcttg gcgcgggaat
      241 ttatacaatt actgttaccg atggcaatgg ttgcactatg agctcgagcg ctacaataac
      301 atcgccatca gctcttggct gtatttgcaa tagcagtgta accaatgtaa gttgttttgg
      361 tggcagtaac ggaagtgtta gtgctcttcc attaggaggt acagcgccat ataattattt
      421 atggaacaat ggcgctacaa ctcaaagcat aagtggtctg gtagctggaa cctatactgt
      481 aattataacc gatgcgaatg gttgtacaaa agtaggtaca gcaacggtaa cccaaccttc
      541 tcaattgttt gtatttgtgg cagggcaaaa cttaacttgt gcaggaaata acacaggtgc
      601 tgccactgct acaccatcag gtggagttgc accatataca tatctatgga caccagcagg
      661 acaaactaca caatccgcaa ctggattagc ggcaggaaac tatacagtta aagtaacaga
      721 tgcaaatggt tgtacagcaa cagctcaggt aactatcact caaccaccat caatgacatt
      781 aacatccaag cgtacaaatg ttagctgctt tggtggattt aatggaacat ttactgtagt
      841 ggttaatgga ggtactcctg cttattctta tttgtggtcg aatggaaaaa ctaccaaatt
      901 tataatagga ttaggtgctg gagcgtatac cgttacagtt acagatgcag caggttgtac
      961 taaaacattg agtggcataa taacacagcc acctgctatg gtggccttgt atacagttac
     1021 aaatgtaaat tgtaatggtg gatcaaacgg agctatagat ttaactataa caggcggaac
     1081 atctccttac acttatgttt ggagcaatgg cgcattaaca caagatataa cgggtatagc
     1141 agctggaatt tatgcagtaa ccgttaaaga caaaaaaggc tgtattatta atgcaacagt
     1201 tgttgttagt caacccacac caataacctt aagcatgact ccaaaatctc caaaatgttt
     1261 tggcggaaat aatggtcagg ctacggttac cgcttcagga ggaacagtac cttactctta
     1321 cttatggtcg aatggaaaaa ccacttctac tgctaccgga ttgactgcgg gaacatatac
     1381 agttactgtt acggatagta aagcatgttc tgccacaatg agtgttacta tttcgcaacc
     1441 accattactt gcttgtacag cgaatgtgat acaaggcatt ttatgtaacg gtggcttggg
     1501 taaaatagga gttactgcta caggcggcac tccgggatat tcatatacat ggaatacaaa
     1561 tccaataaca acaacagcta tagcaaataa tgttgtagcc ggaacttaca ctgtgattgt
     1621 aaaggataaa aataactgta ctacaagttg tgttgttaca attactcaac ctgctgcaat
     1681 tacttgtgcg cctttaacaa gcacccctgt tagctgtaat ggtgctaatg atggaactgc
     1741 tactgttagt ggaattgccg gaggtacttc tccatacact ttgttatgga acagttttgt
     1801 acctcaaaca acgccaacgg ctacaggttt aagtgcagga acatatactg tgactattac
     1861 tgatgcaaac ggttgtacaa aaacggcaag tgtcatagtt acaggcccaa ctgccttaac
     1921 cgccacttat tcgagtatca gtcctggttg taatggaggc gtaggcacta ttacagtagt
     1981 ggctgccggt ggttctcctg cttacagtta tacagttaat cccggtgcaa tcaataacac
     2041 cacaggagtt tttggtggct tatcggccgg aacgtatagt gttttggtta cggatgctaa
     2101 tttatgtact acagtaattt ctaatattgt aatcacttcg ggtaatacca ttactgcaaa
     2161 tccgcttaca tctacaaatg tgaattgtaa tggaggaaat aatggtactg cgtcaattac
     2221 tggcatttca ggaggtttgg caccgtatac cctaatctgg agcactgttc ctgttcaggg
     2281 tactttaaca gccaccggac ttactgcagg aacgtatacg gtaactgtta cagatgcaaa
     2341 tggatgtatt tatactgctg cggctatagt aaccgaacct tcaacacctg ttacggctac
     2401 aatcaattca caaataaata ttgattgtaa tggaggtgga ctaggagaag ctactgtttt
     2461 agcttcggga ggttcatcca caaattcata ccttgacaat aactttgcag cagcattccc
     2521 aagtggattg gtattaggcg gaacaggagg ttgcggaaca ccttacacgc ttacgtttac
     2581 cagtcctgcc gccataaggg catttattcc taatgcattg ggattggctg ctccattaac
     2641 aatggatatg acagacccaa ctaatggtag ttatattaat tctttagccg gtgctctggt
     2701 agcaactacg ctaaatgtgc agtttgattt atatgatgca aactttgctc ctaccagtac
     2761 agttcatctt gctgatttaa tttnnnnnnn nnnnnnnngg gcatgaccgt tcaacaaata
     2821 ctggatgaaa gtaataatgc tctcgctggt tgtggaagta tttatactcc taatcaactg
     2881 cgaacagtat tgcaacaaat caaccatagc tgggaaaatg gtgtgcctgg aaatggattt
     2941 ctttcatgcc ctgttggccc tcccggatgc aatacattct attctttaac tcaggaagga
     3001 tgggatgcaa caggcggcta cacttacttg tggagcaatg gacaaacaac agcaactgcc
     3061 acaggattaa ctgccggtac ttatgtggtg actgttaccg atgcaaacgg ttgcacagca
     3121 gtaacccaag taacaattac tcagccggga agcttaacgg taagtgcagc agcaataaat
     3181 gctttgtgta atggaggcgc taatggaatg gtgttggctt ctgctcaact aggaacccca
     3241 ccatactcat ttatgatttc tccaatggca ggcacacaaa caaatcctac tgccgacagc
     3301 gcagtattta caggattggc agccggtact tatactgtta tagtaactga tgccggagac
     3361 tgtactgccg aaacggatgt cactgtaaat gaaccaagcg ctatagcatc caatactact
     3421 cacgttaacg tgtcttgtaa tggaggctca aacggtgtgg caactgtata tgcaataggc
     3481 ggcacacccg gatataccta ccaatggagt aatggaacaa gcgttgtgtc gacaaatcaa
     3541 caagctactg gtttgccagc tggcacgtat actgtaactg taaccgatgc aaacggatgt
     3601 ataaatactg gaagcactac aattgcacag cctgctgtat taagcattac acctacacag
     3661 gttaatgttt cttgtaatgg tcagggaaca ggaagtgcaa ctgccactgt tacgggagga
     3721 actgcaccat ataattattt atggaacaat ggtcagcaat cgagtacagc aacgggttta
     3781 atggccggaa catatacagt taccgttact gatttcaaag ggtgcacaaa tacacagagc
     3841 tattcaataa cttcaggagt aaacattatt gttaacccat ctcaaacgcc aattctatgt
     3901 aatggaggta cgagtgtatt gacagtaaac ccaacaggtg gaacgggaac ttatacttat
     3961 tcatggagtc aggctccgtt aaacactaca aatactgcaa cagtaaatgc cggagtttac
     4021 tatgttactg taacggatag cgcaggttgc tctgtcactt attgttttgt ggttgctcag
     4081 ccaaatgctg ttgcttgcgc tattagcagc acaggggttt cttgtaatgg ttctgcgaat
     4141 ggttctgcaa cagctaatgt tataggagga actcttcctt actcttatag ctggaatgat
     4201 gctaatagcc aagccacaca aacagctatt ggtctgggtg ctggtacgta tacagtatta
     4261 gtaacagatt ctaatggttg tacttcgcag tgcagcataa caattaccga acctcaagcg
     4321 ctttcagtag gaacaagtaa tacgaatgta tcttgtcaaa acccaactgg aacagcagtt
     4381 gctattgttg gaggaggtac agcaccatac acttatttat ggagtaatgg agcaagtact
     4441 tctgctatta caggtttgtt gccaggaacg tatactgtta ctgttaatga ttctaaaggt
     4501 tgtggaccag taactgctca ggcaactatt cttgcaccgg ttgctgttac ttgcagcgca
     4561 acatcaacta tgacctcatg taacggatat cacaatggca ctgccactgt tactccgggt
     4621 ggaggtaatg gcgtttattc tgtaatatgg aataccacac ctgcacaaac aacactaaca
     4681 gctaccggca tgtcggcagg tacttattct gtggttgtat tagatggttt gggttgtagc
     4741 tcaacatgta cagttgtagt tcaggaacct gctcctttgg tttactctta taatattaac
     4801 aacacatgtg caggtagtaa tggaggaacc tctactatta atgcgatggg aggtactatg
     4861 tacactaacg gtaaccctta tacctacttg tggaacaatg gacaaactac tcagaccgct
     4921 accggtcttg gaagtggaac atattatgtg acagtaaccg acagtttggg atgtgcaata
     4981 caagctgtga ttataatgcc tcaagcacaa agcatgactt gcaatacatt ctgtacacct
     5041 acagcatgtg ggcaggcaac cggaacagct accgtgaatg tatttggcgg aactatgcct
     5101 tacacttatc agtggagcaa tggtaatacg aatgtaatca ataccggatt gacagccggt
     5161 acttatacag tgcttgttac cgatgcgcag ggatgtacca caatgtgtat tgccaatgtg
     5221 ggtcaaccgg caaatataca atacacatta gcagtggtta accctgcttg taatggagga
     5281 acaggaagtg tgactataac taatcttaat ggtggaatgt ctccttactc atacctctgg
     5341 aataatggct caactgcact ttcagtatcg ggccttgcag gaacgtatac tgtaacggtt
     5401 accgatataa atagttgcag cattactatg actgctacta ttgttcaacc gcttatttta
     5461 aatgttacca cttcggctgt gaatgttact tgtaatggat acacgaatgg aagtgctgct
     5521 gctgttgttt cgggcggtac tgcacctttt aactatcttt ggaataccgg tggaaattta
     5581 agttacatga ataatttggc ggcaggtacc tatactgtaa ctgttacaga tgcaaacgga
     5641 tgtatagcaa ccgctcagtc agttattgct cagccgttag caatggatac tgtaagtact
     5701 tcgagcatat cttctacttg cggattatgc aacggtagcg gctcgttagt aattacaggt
     5761 ggaactgcgc catacagcta tttgtggagc aatggcgcta caagcgataa tgctacaggc
     5821 ctatgtgccg gaaacaactt tgtaactgtt accgatgcaa atggatgcac aataagtgtt
     5881 tgtgtaaaag tgagcaacac aggatcattc gctgccgcgc ttactagccc aacttatgca
     5941 ggtggctata atatccgttg taacggtgga ttagatggag ccgtaaatct tacagtaaat
     6001 cctattggaa cttatactta ctcctggagc aatacttcaa caaatgaaga tttatttggg
     6061 gtaggtgccg gcacgtatac tgtaactgtt agtaacggaa catgtactca aacggtaagt
     6121 attaccctta ccgaagctcc attgttaact gcttcggcta ccggcacaaa tgcaaattgt
     6181 agcggtacta atacaggcac agcaactgct ttgggcattg gcggagtgat gccgtatacc
     6241 tatgcttgga gcaatggcca aaccacccaa acagcaaccg gattgatggc aggaacatac
     6301 actgtaatta taggcgatgc caatggttgt actgctaatg caacatatac agttacagga
     6361 gttgtaaatg ctttatcaat caccgaaacg catgttaatc ctacttgtta tggcaataca
     6421 aacggcagta ttgacataac tgtcactggt ggttcattgc catatactta tatttggaca
     6481 ggaggagcaa caacagaaga cagaacaaat attggagccg gaagtcatac agtaacagta
     6541 acagatgcaa atggatgcac tattacgctt tgtgtggtgg taacacaacc aaatgctata
     6601 gtgatatcta tgagtacaac caatgccact tgcaacggtg gtagcaatgg ttcggctgcg
     6661 gcagcagttt cgggcggcag ttcgccatat acttatcttt ggaatacagg tgctaacaca
     6721 gccaacataa atggacttgc agcaggtacg tataccgtaa cggttacgga tgccagcggc
     6781 tgtactttca gtgcaaacaa agccgtaggc gcaccagtga gtatgactgt aattgttgat
     6841 agtgtaaaaa atgtaactgc caacggtggt aataatggag cagcttacat atcattaaat
     6901 ggtggtacag ctccttttgt ttatagctgg aatactattc cggtaaaaac aacacaggat
     6961 gttacaggct taaccgctgg tacttatcag gtttcggtta cggatgctgc aggatgtgtt
     7021 gccaatgcaa ctgtgacaat tacgcaacca aactatatat gtagcaacgg aacaccattc
     7081 cataccgaaa cgcctgcagc ctggactgtg gccggaaatg gaagtaatat tggcacttat
     7141 ttgacattgc atttccctaa tgcgttccca ggttcattgg ttgttggtgg cggttgttta
     7201 ggtagtaaaa cattgacatt aactaacgca acagccgtta gaagtttctt gttaggacag
     7261 ccattaactg gtggtgctaa tttgttatcg agcaatttga ataatcctac caatgcaaca
     7321 tttagtagtg cattagcagc aaatattgtg gctcttaaat tagctgtggt atttgatgca
     7381 tacgatggaa actttgctcc tgttccaaca gtttggcttg ccaatttgat ttacaataat
     7441 cctgcaggac ctgctgcctt aaatggcttg acagtcaatc aggtattgtt cgaagcggag
     7501 aaaaaacttg gtggttgcag ttcttcattt actggcgcac aattaacaac tgcgttgaat
     7561 aacatcaact tgagctacct tggcggtgtt gcaggtgcat cttctacaat gcttacctgt
     7621 cctgcaccac caaaattagg tgatgtggta actacgattg gcaacattgt ggcatatcct
     7681 aatccaaccg aaggtcaatt aaatatacgt tttgtaacag agcaagatgg taaagtatcg
     7741 gtacgattat ttgatgcgat tggaagatta gcattcaaca ccactgaaac agcttttgaa
     7801 ggtgtgaacg aacgttcata cgatgtaagc aatttatcaa aaggtgttta catgttgaat
     7861 gtgcaggtaa atggcctgac tgaaaccata cgagttgtag tcaaataaat tattagaata
     7921 tatttcttaa cactaaaacg cctcgcattt tgcggggcgt ttttggttta tataggggca
     7981 atatattttc gaaattattt tttgttcgta ggatttcttt ttgaaaacat aagagaaagg
     8041 tgaaactaac cataaccgta tacgcagatg caaaatggca gggtgcgaag cgcattgtat
     8101 gttgctggaa cggcaacata ccggagaggc cacc
//
LOCUS       DAORQA010000067         8012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2367_length_8012_cov_9.085711, whole genome shotgun sequence.
ACCESSION   DAORQA010000067 DAORQA010000000
VERSION     DAORQA010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2367_length_8012_cov_9.085711"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>560
                     /locus_tag="PK736_05270"
     CDS             <1..>560
                     /locus_tag="PK736_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009413146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRC32834.1"
                     /translation="IGKSAIIAQKIVATFNSTGTPAVFMHAADAVHGDLGIIQQSDTI
                     ICISNSGNTPEIKLLAPILRNAGNKLIAITGNTESVLAKEAHYVLYAGVTTEACPHNL
                     APTSSTTAQMAMGDALAVCLLQCRGFSKGDFARYHPGGALGKKLILTVDDFYKKNPVP
                     AVDINAGIDKVILEISSKRLGATAVL"
     assembly_gap    561..570
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <571..822
                     /locus_tag="PK736_05275"
     CDS             <571..822
                     /locus_tag="PK736_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009413146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRC32835.1"
                     /translation="NKKLAGVITDGDLRRMLQKQNNFLEMKASDIMNANPKTIVPGTM
                     AVDAFNLMKEKNITQLIIAKGSNYLGIIHLHDLLQDGVY"
     gene            841..1308
                     /locus_tag="PK736_05280"
     CDS             841..1308
                     /locus_tag="PK736_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32836.1"
                     /translation="MELNEKEKFIYTDAINFFKTLKGDEHPAWGLMNAQQMIEHLCDS
                     VAIANGNAPLPMHTAEDQVAKWKAFAMSDKDFKPNTPNALMGENPPAVQHISITVAIK
                     QLQNELASLHEFYKTNPERTEMNPFFGPLNYAEWVHLFHKHFRHHLRQFGLID"
     gene            1409..1831
                     /locus_tag="PK736_05285"
     CDS             1409..1831
                     /locus_tag="PK736_05285"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRC32837.1"
                     /translation="MFRYFVAAGVATVVDIGVYYIMLHYVFQNRLFTIAPYLTVSSPS
                     LSLFISYTCGLFTNFFISKYFVFTESEVRSRHQFFRFAGVALSVLFLNYCLMSLLIHR
                     LEWYPTIARTVSALSLGIVSYSFHKIFTFMVSKRVRLR"
     gene            complement(1861..3522)
                     /locus_tag="PK736_05290"
     CDS             complement(1861..3522)
                     /locus_tag="PK736_05290"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32838.1"
                     /translation="MKTIYFLILTLLLMSGQTYSQPISLRTDITITKIIQAQKRCARI
                     EFDKVQNKLFTLTTKCMLYGIDSVPGMGWKDSLYADIGDIQIDFPTGMAFKDSTLYII
                     GNNWVDTTGTEGIIRGGILQPNGTRAWYTVMQTDTYPQANTFFDHGMSCIVLNPTGDS
                     LYINSGSRTDHGEVQSNYNTYPGARDVPITSCILRVPANAQNLLLHNDSAFLIPYTFC
                     DGTRNEFDMKFLPNGDLIGAENSGDRDHTDELNFLQEGKHFGFPWKMGNDMNPQQFSN
                     YDPNTDLLINHKRKSWQLGYFYNDTTFPPPPPNITFIDPIQNYGPNGDKFRDTITGQM
                     MDANDLGISIGSFTSHRSPVGLIFDNDSLLCNGLTGNGFMLSYMPGADSLGNTGAVGF
                     GAPADTSGDMLALRLVKNNATNIYTMQSTRVIYNFDNPSDACMVGNIIYVVEYATDTT
                     ADIWRIEMPVVANCGAALNEWTSTNNELEIFPIPFTNEINIKCKQCNSPSSLIIYNAD
                     GKILLSYILTVNETKINPELTAGIYFAEWICQNEKMYKMIVRMRE"
     gene            3791..4126
                     /locus_tag="PK736_05295"
     CDS             3791..4126
                     /locus_tag="PK736_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010135875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HRC32839.1"
                     /translation="MEQVSYETFAALDIRVGTVLEAEVFDKARKPALKLKIDFGVLGI
                     KKSSAQITEYYAPENLIGKQIVAVVNFAPRQIADFMSECLVLGIYDLNGAVVLLQPER
                     AMLNGQKIG"
     gene            complement(4144..4593)
                     /locus_tag="PK736_05300"
     CDS             complement(4144..4593)
                     /locus_tag="PK736_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019038108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRC32840.1"
                     /translation="MISKKAKYALKALEYIAKHNEQGPILISEISERQRIPKKFLEVI
                     LLELKRDGILQSKMGKNGGYYMLKDPKDVNIGHIIRLIDGPIALLPCVSYKYYEKCAE
                     CEDEATCGLRNLMAQVREANNKILNKVSLAEIVKRESMLQKGLTITL"
     gene            5014..5085
                     /locus_tag="PK736_05305"
     tRNA            5014..5085
                     /locus_tag="PK736_05305"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5047..5049,aa:Glu,seq:ttc)
     gene            5201..5455
                     /gene="rpsT"
                     /locus_tag="PK736_05310"
     CDS             5201..5455
                     /gene="rpsT"
                     /locus_tag="PK736_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRC32841.1"
                     /translation="MANHKSSEKRIRSNDAKKVRNRYQAKTMRNALKDIRTTKDKTEA
                     AKLLPQLTTMLDRLAKTNVIHKNKAANLKSSLAKQFNKMS"
     gene            complement(5702..7069)
                     /locus_tag="PK736_05315"
     CDS             complement(5702..7069)
                     /locus_tag="PK736_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32842.1"
                     /translation="MNKIRIGGLLLCMQISVAMGQVGSISPYSQYGLGDLQHHGFVSQ
                     LGMGGCGVALNDTLQINIANPASYASLKVTLFDAGLNASVNRLSTSNKFTNKTDAAFS
                     YFALGFPVVRGGKLGGCFGLIPYSTRGYSVNYNTLNTQGDKVTHYFEGSGGVNRFFVG
                     LGSKVWRNFSVGGHASYLFGTLSKTRRTEYADNTFLYARNTLNTSITDFMFDAGAYYK
                     TDSLFSVRTKKHDTKNPGTDSLPNYIYKKKYIRFSLGAASTFQTNLKDKNDFITETYY
                     TDPLGTIYLVDTAEYIPTVKGKTTLPASYRVGFSLQNVNHWIYTAEYQLQDWSKFTSF
                     GINDNLQSSFGVSTGFEFIPKFNSPKYGKRIAYRFGFRYNQSMLNLRSTPITDMTLSF
                     GVGLPIRKDAVTAPYATSFSRINLSLEVGKRGTTTNNLLLEEYIRLHVGVCLSELWFI
                     QRKFD"
     gene            complement(7062..7901)
                     /locus_tag="PK736_05320"
     CDS             complement(7062..7901)
                     /locus_tag="PK736_05320"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRC32843.1"
                     /translation="MAQPSPSSKLISTAKSTAQFDCVIDAGNTNVKIAFFDNNDLQIG
                     KTISSPYSHNWVADIITKNKNKIAHTIYSSVRNDDTKTINWLQQDKNFHLLSLKSKMP
                     FINKYATPATLGNDRKANMAAATAIAPNKNVLVIDAGTCIKLDMLQADNTYFGGAIHP
                     GIDMRYKALHDYTGKLPLLGMNEKKTKKLTGNRSSVSNTEFELVIGTTTEQSMRAGVE
                     IATVNEIKSCILAYRKKCKNLQTIITGGDMRFFAEQMNFTFAIPNLTLKGLHTILKIN
                     MNE"
     gene            complement(7905..>8012)
                     /locus_tag="PK736_05325"
     CDS             complement(7905..>8012)
                     /locus_tag="PK736_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC32844.1"
                     /translation="KDFPVEKFYRDAKLCTIGEGTSEIQKLVIAREILK"
BASE COUNT         2420 a         1502 c         1550 g         2530 t
ORIGIN      
        1 gtattggtaa gagtgccatc attgcgcaaa aaattgtggc tacattcaac tccacaggaa
       61 cacctgctgt gtttatgcac gctgccgatg ctgtgcatgg cgatctggga ataattcaac
      121 aaagcgatac tattatatgt atatcgaata gtggaaatac gcccgaaata aaattgctgg
      181 ctcccatatt gaggaatgcc ggcaacaaat tgatagccat cacaggcaat accgaatcgg
      241 tattggcaaa ggaagcgcac tatgttcttt atgccggtgt aacaactgaa gcatgccccc
      301 acaatctggc accaacaagc agtaccaccg cacaaatggc catgggcgat gctttggcgg
      361 tgtgcctgtt gcaatgccgt ggctttagca aaggcgattt tgcgcgttat catcccggtg
      421 gggcattggg caaaaaatta attttaacag tggacgattt ttacaaaaaa aatcctgtgc
      481 cggctgtaga tattaatgca ggtatcgata aagtgatatt ggaaatttcg tcaaaacggc
      541 ttggggctac cgcggtattg nnnnnnnnnn aataaaaaac ttgcaggtgt aattaccgat
      601 ggcgacttgc gaagaatgtt gcaaaagcaa aataattttt tggaaatgaa agccagcgat
      661 attatgaatg ccaatccaaa aacaatagtg ccgggaacca tggctgtaga tgctttcaac
      721 ctgatgaagg aaaaaaatat tacgcaactt ataattgcca aaggctccaa ctatttgggc
      781 atcattcacc ttcacgatct tttgcaggat ggtgtttatt aatctttaaa aaactaaaca
      841 atggaactaa acgagaaaga aaaatttatt tataccgatg ctatcaattt ttttaaaaca
      901 ttgaaaggcg atgaacaccc tgcatgggga ttgatgaacg cccaacagat gatagaacac
      961 ctttgcgata gcgtggccat tgccaatgga aatgcgcctc tgccaatgca tacagccgaa
     1021 gaccaggtag caaaatggaa agcttttgcc atgagcgata aagattttaa acccaacacc
     1081 cccaatgctt tgatgggcga aaacccgcct gcagtgcagc atatatccat tacagttgct
     1141 ataaagcagc tgcaaaacga actcgcctca ttgcacgagt tctataaaac caatccagag
     1201 cgaacagaaa tgaatccttt ttttgggcca ctaaattatg ccgagtgggt tcatttgttt
     1261 cataaacatt ttagacatca cctgcgccag tttgggctta tcgattgatt tttgtcttgt
     1321 tttcaaacat gcataattcc gctatatttg cgccctttaa ttttttcgga gaatgcgaat
     1381 tcttgtaaac aattttttta actctcgtgt gttcaggtat tttgttgcgg ctggtgtggc
     1441 taccgttgta gatattggcg tctattatat tatgttgcac tacgtttttc aaaacaggtt
     1501 gttcaccatt gctccctacc ttacggtttc atctccttcg cttagccttt ttatttcgta
     1561 cacctgtggg ttgtttacca acttttttat cagcaaatat tttgtcttta ccgaatcgga
     1621 agtgcgcagc cgtcatcagt tttttcgctt tgccggtgtg gccctttccg ttttgtttct
     1681 caattattgc ttgatgagtt tactcattca ccgcttagag tggtacccaa ccattgcacg
     1741 aactgtttcg gcattatcac tgggaattgt aagctacagc tttcataaaa tctttacttt
     1801 tatggtaagt aagagagtga gattgcggta attttcttta agtataaaat tttatttttc
     1861 ctattctctc atcctcacaa tcatcttata cattttttcg ttttgacaaa tccattctgc
     1921 aaaataaatt ccggctgtaa gctccgggtt tatttttgtt tcattaactg tgagtatata
     1981 cgataataat atttttccat cggcattata aataattaaa gatgatggcg aattgcattg
     2041 tttacatttt atatttattt cgttggtgaa tggaattgga aatatttcaa gttcattatt
     2101 tgttgaggtc cattcattta gtgcagcgcc acaatttgca actacaggca tttcaatacg
     2161 ccatatatca gctgtggtat cggtggcata ttctacaaca taaataatat taccaaccat
     2221 acatgcgtca gaaggattat cgaaattata aatgacccga gtagattgca tggtgtaaat
     2281 attggtggca ttatttttta ccaaacgcaa tgcaagcata tcgcccgaag tatcggcagg
     2341 ggcgccaaag cccaccgcac cggtgtttcc caagctatcg gcaccgggca tataactgag
     2401 cataaatcca tttccggtaa ggccattgca aagcaaacta tcgttatcga aaattaaccc
     2461 caccggtgat cggtgcgatg taaaggaacc tatacttatt ccgaggtcat tggcatccat
     2521 catttgtcct gtaattgtat cgcgaaattt atcgccattg ggtccgtaat tttgaatggg
     2581 atctataaaa gtaatatttg gcggtggagg cggaaatgtt gtatcgttat aaaaatatcc
     2641 caattgccac gatttgcgtt tgtggttgat aagcaaatca gtattaggat cgtaatttga
     2701 aaactgttgt gggttcatat cgttccccat tttccatgga aagccgaaat gttttccttc
     2761 ttgcaaaaaa ttgagttcat cggtatggtc tctgtcaccc gaattttcgg cacctatcaa
     2821 atcgccatta ggaagaaatt tcatatcgaa ttcgttgcgc gtgccatcgc aaaaagtata
     2881 tggaataaga aatgcagaat cgttgtgaag taataaattt tgtgcattgg ctggcactct
     2941 caatatgcac gaggtgatgg gcacatcgcg cgcaccgggg taggtattgt aattgctttg
     3001 cacctcaccg tggtccgttc gcgagccgct gtttatatac aaactatcac cggtaggatt
     3061 taaaactatg caactcattc catggtcgaa aaaagtattt gcttgtggat aagtatcggt
     3121 ttgcataacg gtgtaccatg ctcgagtacc attgggttgc aatattcctc cgcgaataat
     3181 tccctctgta ccggtagtat caacccaatt gttgccaata atataaagtg tagaatcttt
     3241 aaatgccatg cctgtgggaa aatcgatttg aatatcgcca atgtcagcgt ataaagaatc
     3301 tttccaaccc atgcccggca ccgaatcgat tccatataac atacatttag tagtgagcgt
     3361 aaataatttg ttttgaactt tatcaaattc aatacgtgcg cagcgttttt gtgcctgaat
     3421 aatttttgta attgttatat ccgttcgcaa cgaaatgggc tgagaataag tttggccaga
     3481 catcaataaa agtgttagaa tcaaaaaata tattgttttc atgaacttag ttttttgtgt
     3541 aaagatactt ttcaaatagt gtttaatatt ttcaataaaa atggctctgc tattaattaa
     3601 tatcgtgtgc aagttaagca aatgctttta tgacatccat gagaaaatga attgctaaag
     3661 cacctgaccg aaaatcttgt tttgtttcac tatcaaactt tatgactttg ttgtttctaa
     3721 ttttatactt ttgaatgtcg attagaaata agaaaagaaa gcaaaagttt gaaaaaaaag
     3781 tattttcaat atggaacaag taagttacga aacctttgca gcgcttgata ttagagtagg
     3841 aacagtattg gaggcagaag tgtttgataa agcacgcaaa cctgcattga aactgaaaat
     3901 agattttggt gtgttgggaa taaaaaaatc atcggcacaa attaccgaat attatgcacc
     3961 cgaaaattta attggtaagc aaatagttgc tgtggtgaat tttgcaccac gccaaattgc
     4021 agactttatg agtgaatgcc tcgtacttgg tatttatgat ttaaatggcg cagtagttct
     4081 tttgcagcca gaacgcgcta tgctgaacgg acaaaaaata ggatagtaat ttttaagttt
     4141 tttttacaaa gtaattgtca ggcctttttg caacatgctt tcgcgtttta caatttctgc
     4201 aagcgatact ttattcaata ttttattgtt ggcctcgcgt acttgtgcca ttaaattgcg
     4261 caatccacaa gtggcttcat cttcgcattc ggcacatttt tcatagtact tatacgatac
     4321 acatggtaac aatgcaatag gcccgtcaat taagcgaata atatgtccaa tgtttacatc
     4381 cttggggtct ttaagcatat aatacccacc gtttttcccc atcttacttt gcagtattcc
     4441 atcgcgtttt aactcgagca aaatcacttc cagaaatttt ttgggaatgc gttggcgctc
     4501 tgaaatttct gaaataagta taggcccttg ttcattgtgc ttggctatat attccagggc
     4561 tttaagcgca tatttggctt tttttgatat cataaatttt agtttgggaa ttcaactagg
     4621 ggcaaatgta atttaatagt ttgataataa aaggtttaca aatccattcg ccaaatgtag
     4681 atggcttgat aatagtatgc tgtggttaac ttgtttactt tcgatggtaa aataaagata
     4741 acctgttatg gatatatttt gaattataat ttgtaggacg caaatacaaa ttgttttatt
     4801 gcaaaacttt agcaaataat agaatgtaag cattgttcta tgtgatagtg tttaatatcc
     4861 cataagtttg atttatttaa ttaaacgata gaaaagtagg aattggttaa tattactgtc
     4921 aattacagtg agtcgctact tcaaaattat ttctatttaa tttgttgtgc aataaagaaa
     4981 taaatctact tttgcaccct cattttaaaa gatggtccgt tcgtctaggg gttaggacgt
     5041 atccctttca cggataagac acgagttcga ttctcgtacg gactacaaac aaaggatttt
     5101 taggaaatcc aaaatttaaa aaacggaaag caaagcaggg cgaaactcct gcattgctct
     5161 cccaaaacaa gaagtaaaaa aaataaactg tcgataaaca atggcaaatc acaaatcctc
     5221 cgaaaagcgc attcgtagca atgatgcaaa gaaagtacgc aaccgctatc aggcaaaaac
     5281 aatgcgtaat gcgttaaaag atatacgtac tacaaaagac aaaactgaag ccgcaaaact
     5341 tttaccacag ctaacaacaa tgctcgaccg tcttgcaaag accaacgtta ttcataaaaa
     5401 caaagctgcc aacctaaagt caagtttagc aaagcagttt aataaaatga gctaactatt
     5461 cgagctgaat tttgaagcct ctttatttca gcaaactata ttgcccttca ttttatggag
     5521 ggtttttttg tgcttattaa ctcacgtttt gaagtaaaag gagatcatta agccgcacta
     5581 tttgaattag tttggttagc accaatttag aaaatcactc aattgttgaa atccaatgga
     5641 accaaaaggg ttgaattatt aatcgtttta caatcgataa tttcaattgc ataatttggg
     5701 attaatcaaa cttacgttga ataaaccaca attcgctcag acatactcca acgtggaggc
     5761 gtatgtattc ttccagcaat aaattgtttg ttgtggtacc tcgcttgcca acctccaaag
     5821 ataaattgat gcgtgaaaaa cttgtagcat atggcgctgt aacggcatct ttgcgtattg
     5881 gcaaccctac gccaaatgaa agtgtcatat cggtaatagg agtggacctt aaattaagca
     5941 tgctttgatt gtatctgaac ccaaacctgt aagcaatgcg cttaccatat ttaggggaat
     6001 taaatttggg aataaactcg aaacctgtac tcacgccaaa actcgattgc aaattatcgt
     6061 ttataccaaa tgatgtaaac ttcgaccaat cctgcagttg atattcggca gtataaatcc
     6121 agtgatttac gttttgcagt gaaaacccaa ctctgtatga tgccggcaaa gtggtttttc
     6181 cttttactgt tggaatatat tcggcagtgt caactaaata aatggtgcct agtggatcgg
     6241 tgtaataggt ctcggtaatg aaatcgtttt tgtctttaag attggtttga aacgtggaag
     6301 cagcacctaa actaaaacga atatatttct ttttgtaaat atagtttggc aagctatcgg
     6361 ttccgggatt tttagtatcg tgcttttttg ttcttaccga aaataaagaa tcggttttat
     6421 aatatgcccc ggcatcgaac atgaaatcgg ttattgaagt gttgagtgtg ttgcgggcgt
     6481 atagaaatgt attatcggca tattcggttc tgcgggtttt actcaatgtg ccaaacaaat
     6541 aagaagcatg tccaccaacc gaaaaattgc gccaaacctt gctaccaagc cctacaaaga
     6601 aacgatttac tccaccactg ccttcaaaat aatgtgtcac tttatcgccc tgagtattga
     6661 gtgtattgta atttacgctg tagccacggg tagagtaggg aataaggcca aagcatcctc
     6721 ccaacttgcc tccacgcact acaggaaacc ctaaagcaaa gtagctgaat gctgcatcgg
     6781 ttttatttgt aaatttgttt gaggtagaaa gtcggttaac cgaagcattc aatccggcat
     6841 cgaaaagagt tactttcaac gatgcataac ttgcgggatt ggcaatatta atttgcaagg
     6901 tatcgttgag ggcaacgcca caacctccca tgcctaactg acttacaaag ccatgatgct
     6961 gcaaatcgcc aagaccatat tgagaatatg gagatatgct accaacctga cccatggcca
     7021 ctgatatttg catacataac agcaatcccc caatccgtat tttattcatt catgtttatt
     7081 tttaaaattg tatgcaatcc cttcaaagtt aaatttggga tggcaaatgt aaaattcatt
     7141 tgctctgcaa aaaaacgcat atcgcccccg gttattatag tttgtagatt tttacacttt
     7201 ttgcggtatg caaggataca actttttatt tcgttgacag tagcaatttc aactccggcc
     7261 cgcatgcttt gctcggtggt tgtgccaata accagttcaa actcagtatt cgaaactgac
     7321 gatctgttac ccgtaagttt ctttgttttt ttttcattca tccctagtaa aggaagtttt
     7381 cctgtgtagt catgcaaagc tttatatcgc atatctatcc ccggatgaat ggcaccgcca
     7441 aagtaagtgt tgtctgcttg aagcatgtcg agtttaatgc acgtacctgc gtcaattact
     7501 aacacgtttt tattgggagc tattgctgtt gcggcagcca tattggcttt acggtcgttg
     7561 cctaaagtag caggggtagc atacttatta ataaatggca ttttcgattt tagagatagc
     7621 agatgaaaat ttttatcctg ctgcaaccag tttatggttt tggtatcatc gttacgcacg
     7681 ctgctgtaaa tagtatgtgc aattttattt ttgtttttcg taataatatc agctacccaa
     7741 ttatgactgt agggcgatga aatagttttg ccaatttgca aatcattatt gtcaaaaaaa
     7801 gcaattttta cattggtatt gccggcatca ataacacaat caaattgtgc tgtggatttt
     7861 gccgttgaaa ttaactttga agatggagag ggttgtgcca atttttattt gagaatttct
     7921 ctggcaatta ctaatttttg aatctcgctc gttccttcgc caatggtaca aagttttgca
     7981 tcgcgataaa atttctccac cggaaaatct tt
//
LOCUS       DAORQA010000068         7985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2376_length_7985_cov_10.796217, whole genome shotgun sequence.
ACCESSION   DAORQA010000068 DAORQA010000000
VERSION     DAORQA010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2376_length_7985_cov_10.796217"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="PK736_05330"
     CDS             <1..1313
                     /locus_tag="PK736_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HRC32845.1"
                     /translation="QLFPTCCTPPHPPEGAIHSAPGAANAHRWSRAVFKNFTVKDGLQ
                     SNEFNRYAYCKLQTGELAFGGVNGLNIFNPDDINDNVYEPNILFTGFKIGNKEVSILE
                     DSAILRKAIFATNQLHLNYTQNFLTFSFASTDYSSTKNNGFQYQLVGLDKGWLPVTKL
                     NEATYTNLDPGSYTFKVRGTNSDGVWSSNIASINIFISPPWWATWWFRSLVAMAVAGA
                     AYGFYRYRLMQALKIANVRNSIARDLHDEIGSTLSSIALYGASAKMMLPEEHPISNAL
                     TKINESTSSMNESMSDIVWAINTRNDKFDNIVSRMEAFANQVMEVRACKVFFATNNEV
                     KQLDLDMEQRKNLYLLYKEIVNNAGKHSSCKNLWVKLNLNNGFLNLNIKDDGKGFNTN
                     ETDAYAMGGNGLFNMKKRAAELHAEIKIDSTPGNGTEVNLKMRV"
     gene            1369..2010
                     /locus_tag="PK736_05335"
     CDS             1369..2010
                     /locus_tag="PK736_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC32846.1"
                     /translation="MKIKVVVFDDNKTLRDGLQMMISLSGSLECIGAFTDCRNVVNVI
                     SELQPDVVLMDIDMPYVDGLQGLNLLRKKFPDLKIIMQTVFEDDDKIFAAICAGADGY
                     LLKRTQPHDLIKGIIEVMEGGAPMTPIVARQVLRLFNNKNNIPKSIEKFELTARELEI
                     LGYLVKGLSYKMIAEKCFVSFATISTHVRHIYEKLQVHSVSAAVAKALEHKIF"
     gene            complement(2261..2473)
                     /locus_tag="PK736_05340"
     CDS             complement(2261..2473)
                     /locus_tag="PK736_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32847.1"
                     /translation="MSHTEFDGFAEGDETIAVYDVLGKQTMATNFSVITGVNQLPIKL
                     DNFAKGIYSVKVTLNGYSRMEKLVVQ"
     gene            3157..3633
                     /locus_tag="PK736_05345"
     CDS             3157..3633
                     /locus_tag="PK736_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32848.1"
                     /translation="MKYFFGILACLCSINFALAQTKVKTYAQQPLWIQMIDNPSTNYF
                     EAVKAFETYWQNHEKPVDIEEEMGPISERVNEKSNVEKKEHKRVEKAFAKMNKRQQKK
                     FAKQQQWQQEMIYQCKRFEEWQRDVFAWVQEDGSILTIEQRQKMYEQKLREAAETK"
     gene            3662..7747
                     /locus_tag="PK736_05350"
     CDS             3662..7747
                     /locus_tag="PK736_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC32849.1"
                     /translation="MKNILQLIFALSIIFCSLQVKAQSWSPVSTGAFPTNTSGQIHGI
                     SRVSDVKFHPSISTKMYAVSARGGLFISNNAGNTWTMAPGCDPLTNGLRFASVCVDYN
                     NDQIIYLGTGDLNYYYTGKGVYKSTNGGTTFAASNTGISARLVAEIIIDPSNSNVLLA
                     ATDAGVYKSTNAGATWTLKSASNLQCSQMLLKANAGTRTVYVSTYSGFYRSLDFGDSW
                     TQITTGIYVPAGYTGANGIRIAVTPADSNRVYLAMVAKKGTVFQSTDGGSTFAAMKDS
                     HNPNLTAYSNDTTTDGGQGDYNFMLAADPVNADVLYLGAHMFWKSTNGGSTWSQLTNW
                     WAELHTDMHWVRVNPYNTNEVWTANDGGVWRSTNGGVNWTPKSDGIYGYEIYHGACSP
                     TRRDMISIGTQDNGELFHANGTWYCNRGGDWGSLCQFDYRANSSQVYYLGNAKRADVL
                     GSDQSYGLTQTSINGLSFWRNNTNIACVSDTAVYRTSTLLQATPVWTKISSFNKTTRD
                     VHINVNDSNKVYAIGSDSAIYICNNAFAVSPLFTRKKIHTSTISIGSIATIKGSPNVI
                     YATANSKVYRSADDGNTWTNVSSNLPSVNWVGIVSDEFFSSDELVFIAGGNYVYYKKA
                     SQTNWTLFNSSLPVRTNIQDISLYDNGTNQSILRVSEYGRGMWEIPMSQLRAISSEFI
                     ASNLLPCIGDSVQFSDLSNGNTISRTWTFTGGSPSTSTLSNPKVAYASIGTYDVTLTV
                     NDALGNATTTKTAYINTAGSNAAVNEGFENTFLPVNWTEQDANNDNNKWQWFTSTGGF
                     GASYKCAYFNNYDQQTNGAWDEMRTQKIDLNGYNTASLTFDVAYQPYGYPYSDTLEVL
                     ISTNCGNTYSQIYIKGGSTLATVGGNNSDAFVPTSSQWRTETISLNGFIGNGVNIAFR
                     NRGRFGNNLYIDNVNLNATVAATAGADKLICKGNTAAIGMSTNSNLNFSWSPAGGLSS
                     STVSNPQASPTVNTSYILTVTHARSGVIAKDTVMVTVDSVTANATTTAVLCNGGNSGQ
                     ASVSTTSGIAPFAYLWSNSSSTPTATGLVAGVYTVTITDNAGCSKTCNVNISQPTLLS
                     ATTTNTNTTCGFNNGTANVLATNATPPYTYLWNNGSTSANVSSLAAGTYTVMVNDGNG
                     CTITATSIINLSVAISPNPTQKNLPCFGAKSGVCKTAPTNGVAPYSFLWSTGATIDSI
                     VNLKAGTYTCTITDAAGCTKTQTYTLTQPLQIAIAMSGTPATSPLFNNGTATATPSNG
                     FSPYRYSWNTSPTKTTQTATGLVPGTYVVTVKDNKKCSRNASIVIGTARFGVTDTGDE
                     LFVIPNPTSDVISIEISNSVLSGNSTFEIIDSKGSVVLMEQ"
BASE COUNT         2633 a         1564 c         1556 g         2232 t
ORIGIN      
        1 accagctttt cccgacctgc tgtacccccc cccacccccc cgaaggggct atacactccg
       61 cgcccggagc ggccaacgcc caccggtgga gtcgcgcggt gtttaaaaac tttacggtaa
      121 aagatggttt gcagagcaat gaatttaacc gttatgctta ttgtaaattg caaacaggtg
      181 aacttgcttt tggtggggta aatggtctca acatttttaa tcctgatgat attaatgata
      241 atgtttatga gccaaatatt ttgtttaccg gatttaaaat cggcaacaaa gaagtgtcga
      301 tactagaaga tagtgcaata cttcgcaaag caatatttgc aaccaaccaa ctgcatctca
      361 attacacaca aaatttcctg acttttagtt ttgcatcaac cgattattca tcaacaaaaa
      421 ataatggttt ccaatatcag ctagttggtt tggataaagg ctggctgccg gtaacaaaat
      481 tgaatgaagc aacatacacc aacctcgatc cgggcagtta tacatttaaa gtgcgcggca
      541 ccaacagcga tggagtttgg agttcgaaca tagcatccat caatattttt atttccccac
      601 cgtggtgggc tacatggtgg tttcgctctt tagttgcaat ggcggtggcg ggcgcagctt
      661 atggatttta ccgttaccgc ttaatgcagg cacttaaaat tgcaaatgtg cgcaacagca
      721 tagcgcgcga tttgcatgat gaaataggtt caaccttaag cagtatcgca ctctatggag
      781 cttcagcaaa aatgatgtta ccggaagaac atcctatcag caatgcactt acaaaaataa
      841 atgaaagcac cagcagcatg aatgaatcga tgagtgatat tgtatgggct atcaacacgc
      901 gtaacgacaa gtttgataac atagtaagcc gcatggaagc tttcgcaaat caggtgatgg
      961 aagtaagagc atgtaaagtt ttttttgcaa caaataatga ggtgaagcaa ctcgatctcg
     1021 acatggagca gcgaaaaaat ctatacctac tgtataaaga aattgtaaac aatgccggca
     1081 aacactcctc ctgtaaaaac ctttgggtta agttgaattt gaataatggt tttctgaatc
     1141 tgaatattaa agatgatggc aaaggtttta ataccaatga aacggatgct tacgcaatgg
     1201 gtggcaacgg attattcaac atgaaaaaac gcgctgccga gttgcatgct gaaatcaaaa
     1261 tagattcaac ccccggaaac ggaactgaag taaatcttaa aatgagggtt taaaaaatca
     1321 acaatattgt tgatatgaaa taagaataaa cgtgtcgata tttgtaccat gaaaataaaa
     1381 gtagttgtat ttgatgataa taaaaccctg agagatggtt tgcaaatgat gataagcctt
     1441 agcggatcat tggaatgcat aggtgctttt accgactgcc gcaatgtagt taatgtaata
     1501 tccgaactgc aaccagatgt ggtattgatg gatattgata tgccatacgt tgacggcttg
     1561 caaggcttaa atttattacg taaaaagttt cctgatttga aaattattat gcaaaccgta
     1621 tttgaagacg atgataaaat ttttgccgct atatgtgctg gtgccgatgg ttatttatta
     1681 aaaagaaccc aaccacatga tttgataaaa ggaattatag aagttatgga aggtggagca
     1741 cctatgacac caattgttgc aaggcaggta cttaggttat tcaacaataa aaacaatatt
     1801 ccaaaatcga ttgaaaagtt tgaacttaca gcacgtgaat tagaaatact cggatacctt
     1861 gttaaaggtt tgagctacaa aatgattgcc gaaaagtgtt ttgtttcatt tgcaaccata
     1921 agcacacatg tgcgacatat ttatgaaaaa ctgcaggtgc attctgtttc agcagctgtt
     1981 gccaaggctt tagagcataa gatattttga cataaacccc aattatgcaa ttgaaattat
     2041 caatggcaaa accattaaaa aaatcaaccc ttttggctcc attgcatttc atcaaataag
     2101 cgattttctc aatcggcaga accccaaatt ttgcttatag ttcggctcgt ttctcaactt
     2161 ctacagcata atttgagttt aataattttt tttgagacaa aaaaaacagc cggtaatttc
     2221 tcaccggctg ctctttcaaa ttataatctt aaaaaagaat ctactgcact actaacttct
     2281 ccattcttga gtatccgttg agcgtaactt ttacactgta aatacctttg gcaaagttat
     2341 cgagcttaat tggtaattga tttactcctg ttatcacact aaaattagtt gccattgttt
     2401 gcttacccaa tacatcgtat acagctatag tttcatcacc ctcggcaaag ccatcaaatt
     2461 ctgtatgaga aataaataac ggttgattta tttaaacagc ctaacgttat aatgaaaatt
     2521 tgcattatta aatgaagcat aatttcattg cacattttta caatttgaaa agcatttttg
     2581 tcgcaccttg ttattaatat ttccggacat gcgcgacttt ttttgaagca tttaattcaa
     2641 tttaaacgaa tacatatgca ttcaatattt cgggaaagta taaagcattc atttgatttt
     2701 tagattttgt tttgtttcga taaaatcaat taaaatgtgg gttaagtaga ttatgaaatc
     2761 aatccaaata tttcatggtt actttagcac acccaaaact tatgcgaagc caataaaaat
     2821 gggttttact taaaacggat tctcaagagt tgcaaattac caaaacataa tctttaaaaa
     2881 aaaactgccc ctcaaaatca actattcgcc taacttttta aactgatgaa gtaaatttat
     2941 tgttgctata gtatggttta agttttatgg cattgcagtt gtcgagtcag ttttaatcta
     3001 acaatttatt aaaagaaaca agcttccttt tgagcgtgca aaatatcttg taaatggtta
     3061 tagttcaaaa ggttgttgga aatattgttc ataataaaac taaaaataaa ccattttatt
     3121 atcttcgcgg aaaattcgac tttaacattt aaaacaatga aatatttttt tgggatttta
     3181 gcatgtcttt gcagtataaa ctttgcattg gcgcaaacaa aagtaaaaac atatgcgcag
     3241 caaccactgt ggatacagat gattgacaat ccatcaacta actacttcga agcagtaaaa
     3301 gcgttcgaaa cctattggca aaatcatgaa aaacctgtgg acattgaaga agaaatggga
     3361 ccgatatcgg aacgggtgaa tgaaaaaagt aatgttgaaa aaaaagaaca caagcgtgta
     3421 gaaaaagctt ttgccaaaat gaataaacgc cagcaaaaga aatttgcaaa gcaacaacag
     3481 tggcaacaag aaatgattta tcaatgcaag cgatttgaag aatggcaacg cgatgttttt
     3541 gcatgggtac aagaggatgg cagcatactt acaatagaac aacgccaaaa aatgtacgaa
     3601 caaaaattgc gtgaagcagc agaaactaaa taataaacca ctgtcaccga aaaaacaaga
     3661 aatgaaaaat atacttcaat taattttcgc tttatcaatt atcttttgct cgttacaagt
     3721 caaagcacaa tcgtggtcgc ctgtttctac cggagcattt ccaactaata caagtggtca
     3781 aatacatggc attagcagag taagcgatgt aaaattccat ccttccatat caacaaaaat
     3841 gtatgccgtg agtgctcgtg gtgggttatt tatttctaac aatgcgggca acacctggac
     3901 aatggcaccc ggttgcgatc ctcttaccaa tggattacga ttcgcatcgg tgtgtgttga
     3961 ctataacaac gaccaaataa tttatctggg cactggcgat ttaaattatt actacaccgg
     4021 taaaggcgtt tataaatcaa caaatggagg cacaactttc gcagcatcaa ataccggcat
     4081 aagtgcacga ttggttgccg aaataataat agacccttct aattcaaatg tattacttgc
     4141 tgcaacagat gcaggagttt ataaatcgac aaatgccggg gccacctgga cattgaaaag
     4201 tgccagcaat ctgcagtgtt ctcaaatgtt attgaaggcc aatgcaggta cacgcacagt
     4261 ttatgtaagt acttactcgg gcttttatcg gtcattagat tttggcgata gttggacaca
     4321 aataactaca ggtatatacg tacctgcggg atacactggt gcaaatggaa ttcgcattgc
     4381 tgtaacaccg gccgatagta atcgggtata tctggcaatg gtggcaaaaa aaggcactgt
     4441 atttcaatcg acagatggtg gttcaacctt tgccgcaatg aaggattcgc ataatccaaa
     4501 tcttactgct tactcaaacg acaccactac cgatggtggc caaggtgatt ataatttcat
     4561 gcttgcagcc gatcccgtta atgctgatgt tttatatttg ggcgcacaca tgttttggaa
     4621 atcgacaaat ggtggcagca catggagtca acttactaac tggtgggccg aactgcatac
     4681 cgacatgcat tgggtgcgag taaacccata caataccaac gaagtatgga cagctaatga
     4741 tggtggtgta tggcgcagca caaatggagg agtaaactgg actccaaaaa gtgacggcat
     4801 ttatggctac gaaatatatc atggtgcttg cagtcctacc cgcagggata tgataagtat
     4861 tggcacgcag gataatggag aattgtttca tgccaatgga acatggtatt gtaaccgcgg
     4921 tggggactgg ggttcgcttt gtcagtttga ctatcgtgcc aatagctcgc aggtttatta
     4981 tcttggaaat gccaagcgtg ccgatgtttt aggctctgac caatcttacg gtcttactca
     5041 aacttcaatc aatggtttaa gtttttggcg caacaataca aacatagcat gtgtttctga
     5101 tactgctgtt tatcgcacat caacattatt gcaagccaca cctgtatgga ccaaaatatc
     5161 atcgtttaat aaaaccacac gcgatgtaca catcaatgta aacgattcaa ataaagtgta
     5221 tgccattggc agtgatagtg ccatttatat ttgcaataat gcatttgcag tttctccgct
     5281 atttacaaga aaaaaaatac atacctccac cataagcatt ggctccatag caacaataaa
     5341 aggcagcccg aatgtgatat atgccacagc aaattctaaa gtgtaccgca gtgccgatga
     5401 tggaaataca tggacaaacg tttcgagtaa tcttccatcg gtaaattggg tgggtattgt
     5461 atcggacgaa tttttctctt cggacgaatt ggtttttata gccggaggaa attatgttta
     5521 ttataaaaaa gcttcgcaaa caaactggac attatttaac agctcactac ctgtgcgcac
     5581 aaatattcag gatatttctc tttacgataa tggcacaaac caaagcatac tgcgcgtatc
     5641 ggaatatggc agaggcatgt gggaaattcc aatgagtcag ttgcgcgcaa tttcatcaga
     5701 atttattgcc agcaatctat taccttgcat tggcgatagt gttcaattct ccgatctttc
     5761 aaatggaaat acaatttcac gcacctggac ttttaccggt ggctctccct ccacttcaac
     5821 tttatcaaac cccaaagtag cttatgcttc aatcggtacg tatgatgtaa ccctaactgt
     5881 aaacgatgca ctgggcaatg ctactaccac taaaacagct tatataaata ccgcaggttc
     5941 aaatgcagca gtgaacgaag gttttgaaaa tacttttctg cctgtaaact ggactgaaca
     6001 agatgcgaat aacgacaaca ataaatggca atggtttaca tccacaggtg gttttggcgc
     6061 aagttacaaa tgtgcctact tcaacaacta tgaccaacaa acaaatggcg catgggatga
     6121 aatgcgcaca caaaaaatag accttaacgg atacaacaca gcatcgctaa cattcgatgt
     6181 ggcttaccag ccgtatggtt atccctactc agatacactg gaggtattaa tttctaccaa
     6241 ttgcggaaat acatattctc aaatttatat aaaaggtggt agtacactgg caacagtggg
     6301 aggaaataat tctgatgcat ttgtacccac ttcttctcaa tggcgtaccg agaccatttc
     6361 gctcaatggt tttataggca acggagtaaa tattgcattt cgtaatcgtg gtcgctttgg
     6421 caataattta tacattgata atgtaaattt aaacgcaact gtggccgcca cagccggtgc
     6481 cgataaactt atttgcaaag gaaacactgc agctattggt atgtcaacaa attcaaatct
     6541 gaattttagc tggtcgcctg ccggaggttt atcatcgagt actgtgagca atcctcaggc
     6601 atctccaaca gtaaatactt cgtatatact tacggttact catgcccgct caggtgtcat
     6661 tgcgaaagac acagtaatgg taacagtaga ttcggtaaca gcaaatgcaa ccacaactgc
     6721 cgtattgtgc aatggtggca atagcggcca ggcaagtgta agcacaacca gtggcattgc
     6781 tccttttgcc tatttatggt ccaatagcag cagcaccccc acagcaacag gtttggttgc
     6841 cggggtttac acagttacca taaccgataa tgcaggatgc tctaaaacat gtaatgtaaa
     6901 tatttcacag ccaacgttgc ttagtgcaac aactacaaat acgaatacaa catgtggctt
     6961 caataatgga actgcaaatg ttttggccac caacgccaca ccgccttata cttacctttg
     7021 gaacaatggc agcacgagtg caaatgttag ttcattggct gccggcacgt atacagttat
     7081 ggttaatgat ggcaatggct gcacaataac tgccacctcc ataataaact taagtgtggc
     7141 cataagtcca aacccaacgc aaaaaaatct gccttgcttt ggggcaaaaa gcggtgtgtg
     7201 taaaacagca ccaacaaacg gtgttgcgcc ctactctttc ttgtggtcca ctggtgccac
     7261 tatcgactcc attgtcaact tgaaagcagg aacttataca tgtaccataa ccgatgctgc
     7321 gggatgcaca aaaacacaaa catacacgct cacacagcca cttcaaatag caatcgccat
     7381 gagtggcacg ccagccactt ctcctctatt taacaacggg acagcaacag ccacgccaag
     7441 caatggtttt agtccttaca gatattcatg gaatacatcg ccaacaaaaa ccacacaaac
     7501 agcaacagga cttgtacccg gcacctatgt agtaacagta aaggacaaca aaaagtgcag
     7561 ccgcaatgcc tctatagtaa ttggcactgc aaggtttgga gtaaccgaca caggtgacga
     7621 attatttgta ataccaaatc caacaagtga tgtcatcagc atagaaattt caaattcagt
     7681 attaagcgga aattccactt tcgaaattat tgatagcaaa ggcagcgtag tattaatgga
     7741 gcaatgaaac atcacttcgc acgattttac tttttcgatt tcgctggcac atttgccaaa
     7801 tggtatttat actttaaggg caaaagattc gaaaaatgtt tttacgaaaa ctgtgttgct
     7861 tgagaagtag ttctaataat gtgccttcat tgctgaagga gtaatttttt ttataaaaaa
     7921 atataggagg tgcaaaattg aagcctatat aattgctggc gaaatacaaa cacagatttc
     7981 tatta
//
LOCUS       DAORQA010000069         7756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2458_length_7756_cov_35.584210, whole genome shotgun sequence.
ACCESSION   DAORQA010000069 DAORQA010000000
VERSION     DAORQA010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2458_length_7756_cov_35.584210"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..3155)
                     /locus_tag="PK736_05355"
     CDS             complement(60..3155)
                     /locus_tag="PK736_05355"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRC32850.1"
                     /translation="MSKSKCKILLLMQESKKAVFSFLLFLLSNYCSIGQTIFDNDIAP
                     TQINYKGYTDLDGLSSNKIIALFQDTAGFLWIGTADGLNRFDGTTFKKYFARIPDYKN
                     SLTDNWIVSLTQYKHYLIIGTLEGISVFNLKSEQFENNYLFDNFRIPNLKANMGFYKV
                     ENYFDLITDFTFSLYNSDLKLLHTIDYTHMKKTNNRFYFGSQMMRILNNKKLQIQANG
                     GYQYLDLANYSLSNIDLIPNNEEEEYKYQQITNQTFFRDSLVFKTIWNSNLSFSDIEN
                     KILHQIIINDTCKYCGDNSKVNEIIKVGKQSILSATGSGMLFYNFKTGSKKLYLIAKE
                     NSAELNQCSSILRDSENNIWIGTLRGLFKITNGINKFQVDAKSPIVANNIRIKKILVN
                     SENEKIIFAENGSTILFIASNNQLSGKYQTPFQTNDIINAIEINNNEYLISTWHGIFS
                     FYRKENYFEPCKYIPDSVSQHVCWGLLKDQHHNVWMGLGGGKGLYRYNIITKETKHFS
                     QYNYAPTDARYLPIRNAMSIVEIDKQHIAFANNQIQNKLCIWNAATDIISEVHASNVQ
                     NKFEPYRGPINSMIAGSSSNIWLATINGIVRYNLHTNTYKQYDKAEGLENSNTSAIAF
                     DNRNNLWVGTNSGLFMLNTITGKFNNFKNDYTVNQLQIDYAFYDSLMHLMYINHNSGY
                     LLFNPESLYTNNRKSKTYFEAIYVNQKPIAIKRDTLFENTENNFQFNFGALNLSDGAY
                     NKFYYQLKPIDTKWHALEYQRQINFSNLLPGEYIFNVTTKNNDEVWGLPVNYHFTIEH
                     AYYQTWWFYLLCSLMVIAVVWQFIRIRLQQIERIYAIRNKLSRDLHDDIGSTLSSINI
                     LSRTAQNNLQQTDNEKTNNTLEKINERSQRLLTNMNDIIWNIKPTNDTMEEVLSRMRE
                     YATSLLEAKKINFEMDFPAEKINYKLTLETKSNLYLIFKEAVNNLAKYSGATQAKLIL
                     NFDNAHIHMKIEDNGAGFNINELKHRGGLTNMKHRAEEMNGKLKIESVIGQGTVVELV
                     L"
     gene            complement(3266..5593)
                     /locus_tag="PK736_05360"
     CDS             complement(3266..5593)
                     /locus_tag="PK736_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRC32851.1"
                     /translation="MKKYFSGILIIMLFVQISVYAQNTFPANGSAGIGTNAPAASSLL
                     EIKSTTKGMLIPRMTSTQRNAIAAPATGLLIYQTNSTPGFYYYTGTAWTAIKTTAGWG
                     LNGNSGTSITNFIGTKDAKPLQFKVNNQLSGKLDYSANANTSYGYKTLINNTGVNNTA
                     LGYQSLGSTTTGGYNTAVGTYSLLNNTLGYQNTAIGSNALVSNQTGNENSALGYGALL
                     NNTTGSGNIAIGNVALNHNTTGNENTAMGVYALYNNTTGGGGVAYGAYALQGNTTGTN
                     NTAIGNSALFSNTSGSNNVANGAGALSSNSYGTENTAIGKWALFSNTGGASNSAHGAE
                     ALYSNTTGLLNTANGYRTLYQNTTGNYNTANGSYSLLFNTVGNQNTATGLNSLQKNTT
                     GSYNTANGSGASYSNTTGNYNTATGYNALYSSTTTDDNTAYGANSLYYNTSGFANVAN
                     GTSALYNNTTGFGNTACGYRSLYLHPEGVTNTGIGDFAEVSTNGIVGATALGSFSVSN
                     ATYKIVIGQSGQATMVIGGYKPWSNLSDGRFKEDVKENVPGLEFINALRPVTYVVDVD
                     KLNHHITAQMPDSMARHYYPDADAIAAAKKNIQTGFIAQEVEATAKKIGYQFDGVNAP
                     TNPTDNYSIAYSQFVPSLVKAVQEVDKENSELKNKVSSLEKTIQQMQQCLNVLCDKND
                     NENKSYIENNSLQIQIAPNPTNGEAQITISGNEKLQNLTIKIIDGAGKLVRSFYASNN
                     SSTFTFDAQLLSKGTYIVQLLNDNEILKTEKLIVQ"
     gene            complement(5623..>7756)
                     /locus_tag="PK736_05365"
     CDS             complement(5623..>7756)
                     /locus_tag="PK736_05365"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRC32852.1"
                     /translation="LLEIKSTTKGMLIPRMTSSQRNAIASPATGLLIYQTTNTPGFYY
                     YTGTAWTALKTTAGWGLKGNAGTNSGVNFIGTTDTASFILKTNNQLAGILSSNTTANT
                     SYGYQALMSNANYNNTAVGYNTLKLNTTAGYNTAVGTYSMLNNTVGYQNTAVGSNSLQ
                     SNQSGNYNAAFGYGALLNNTTGSSNVAVGNTALLQNKTGNTNIAIGSDALNANTTGSS
                     NIGVGSLALFSNTSGSNNIAAGKSALQYNTTGQYNIAIGNSALRINNTDENTAIGFAA
                     LSENTSGTQNTATGAYALTQNDAGYANAAYGYGSLLFNTSGYSNSAFGFKSLYSNETG
                     RDNTACGSQALLNSNGPNADGNSAFGSQALRSNTTGSGNCAMGYGCMSSNTTGLHNVA
                     VGYGAFEYNTSGSANVAVGQRALNWNTTGQRNTAVGEGANTTTFIGTFNNITAIGNEA
                     IVDASNKIRLGDNSVSSIGGQVGWTNFSDGRIKKEIKENVPGLIFINDLRPVTYHFDL
                     NKENELTGISDNSSYPEKYDIEKIAFTGFIAQEVEASAKKINYDFSGVDKSGKIMGLR
                     YAEFVVPVVKSIQELSQQNEDLKKENQEIKKENREIKAAIIDMQNQLDLLASNQKNAL
                     QNSAEIDMVISPNPAHYVANISINSSDKSKTYTLKIFDAVGRQINAYQVIGNAVFELN
                     TSTLNPGTFLVQLFNKTEMIQSQKLVIE"
BASE COUNT         2208 a         1392 c         1339 g         2817 t
ORIGIN      
        1 aaaactaaaa acaacctgtt agacaaaaaa acaaattcat gcaacttgca aagtgctctt
       61 cataaaacca attcaacaac agtcccctgt cctatcacgc tttctatttt caatttgcca
      121 ttcatttcct cggcccggtg tttcatattg gtcaagcctc cgcgatgctt taattcgttg
      181 atgttgaagc ctgcaccgtt gtcttctatt ttcatgtgga tatgtgcatt atcgaaattt
      241 aaaattaatt tagcttgtgt tgcgcccgaa tattttgcaa gattatttac agcttcttta
      301 aatattaaat acaaattgga tttcgtttcg agtgtaagtt tgtagtttat tttttcggca
      361 ggaaaatcca tttcgaaatt tattttttta gcttcgagta aggaggttgc atactcacgc
      421 atgcggctca atacttcttc cattgtatcg ttggttggtt ttatattcca aataatatcg
      481 ttcatgtttg taagcaagcg ttggctgcgt tcatttattt tttcaagggt gttgtttgtt
      541 ttttcattat cggtttgttg caagttattt tgtgctgttc ggcttaaaat atttatactg
      601 ctgagtgtgc tgccaatgtc atcgtgcagg tcgcggctta gtttgttgcg tatggcatat
      661 atcctttcta tttgttgcaa tcgaatgcga ataaactgcc acactacagc aatcaccatt
      721 aagctacata ataaataaaa ccaccatgtt tgataatagg catgttcaat tgtaaaatga
      781 taatttactg gtagtcccca aacttcatca ttattttttg ttgttacgtt aaaaatatat
      841 tcaccaggta ataaatttga aaaattaatt tggcgttgat attccagtgc atgccattta
      901 gtatcaatag gtttaagctg atagtaaaat ttattgtaag caccatccga taaattgagt
      961 gcaccaaagt tgaattgaaa attattttcc gtgttttcaa aaagtgtatc tcgcttaatt
     1021 gcaattggtt tttgatttac atagattgct tcaaaatatg ttttcgattt tctattgttg
     1081 gtgtaaagcg attcgggatt gaataagagg taaccactgt tgtgattaat atacattaga
     1141 tgcatcaatg aatcataaaa tgcataatca atttgtaatt gatttaccgt gtagtcattt
     1201 ttaaagttat taaatttacc tgtaatagtg ttcaacataa ataaaccgct attagtacca
     1261 acccacaaat tatttctgtt atcaaaagca attgccgaag tatttgaatt ttccaatcct
     1321 tcagctttat catattgttt gtaagtatta gtatgcaaat tgtacctaac aatgccatta
     1381 attgtcgcca gccaaatgtt gctactgctg cctgcaatca tgctatttat tgggcctcta
     1441 tatggttcaa atttattctg aacattggat gcatgtactt ctgatattat atctgttgct
     1501 gcattccata tacaaagctt attttgaatt tgattattgg caaatgcaat gtgctgctta
     1561 tcaatttcta ctatggacat ggcattgcga attggcaaat agcgagcatc agttggtgca
     1621 taattatatt ggctaaaatg tttggtctcc tttgtaatta tattgtagcg gtacaatccc
     1681 ttaccaccac caagccccat ccatacatta tggtgttggt ctttcaataa cccccaacat
     1741 acatgttgac ttacagaatc aggtatgtat ttacatggct caaaataatt ttcctttcta
     1801 taaaatgaaa atattccatg ccatgttgat attaaatatt cattgttgtt tatttcaatt
     1861 gcattgatga tgtcgttggt ttgaaatggc gtttggtatt tgccggatag ttgattgtta
     1921 cttgcaataa ataaaatagt ggagccattc tctgcgaaaa taattttttc attttcggag
     1981 tttactaata tttttttgat gcgaatattg tttgccacta ttggactttt cgcatcaact
     2041 tgaaatttat ttattccatt tgtaatttta aataaaccgc gaagcgtacc aatccaaata
     2101 ttattttcgc tatcgcgcaa gatgctacta cactgattta attcagcgct attttcttta
     2161 gcaattaaat ataatttttt actgccggtt ttaaaattat aaaacagcat gccactgcct
     2221 gttgctgaca gtatgctttg cttgccaacc tttattatct catttacttt tgaattatca
     2281 ccacaatact tgcatgtgtc attaataatt atttggtgca atattttatt ttcaatatcg
     2341 ctaaatgata agttactatt ccatattgtt ttaaatacaa ggctatctct aaaaaatgtt
     2401 tggttggtaa tttgctggta tttatactcc tcttcttcgt tattcggtat taaatcaata
     2461 ttgcttaatg aataatttgc taaatctaaa tattggtagc ccccgttagc ttgaatttgc
     2521 aacttcttgt tgtttaaaat tcgcatcatt tgactgccaa aataaaatct gttattggtc
     2581 ttcttcatgt gtgtgtaatc aatagtgtgt agcaacttta agtcactatt gtataaagaa
     2641 aaagtaaaat cggtaattaa atcaaagtag ttttccactt tataaaagcc catatttgcc
     2701 tttaaattcg gaatcctaaa attatcaaac aagtagttat tttcaaactg ctcacttttt
     2761 aaattaaaaa cagaaattcc ttctagggtg ccaattatta agtaatgttt atattgagtt
     2821 aaagaaacaa tccaattatc ggttagtgag tttttataat caggaatgcg ggcaaaatat
     2881 tttttaaagg tggtaccgtc aaatctgttc aagccatctg ctgtgcctat ccataaaaag
     2941 ccagctgtgt cttgaaataa agcaataatc ttatttgaag aaagaccatc aaggtcggta
     3001 tagcctttat aatttatttg tgtgggagca atatcattgt cgaaaatggt ttgtccaatg
     3061 ctacaataat tactaagtaa aaaaagcaag aaggaaaaaa cggccttctt gctttcttgc
     3121 atcagtaata atattttaca ttttgattta ctcactgttg cattgtcaaa taattttttt
     3181 gaaaaatagt ttatttttaa atgagtactg cgtaggttgg tgtatttttt taataacctc
     3241 ttttgatgat taagttataa tcaaattatt gtactatcag tttttctgtt ttcaatatct
     3301 cattgtcatt aagcagttgc actatgtaag ttcctttgct caataattgc gcatcaaatg
     3361 taaaagttga tgaattgttt gaagcataaa acgagcgcac taattttcct gcaccatcaa
     3421 ttattttgat tgtaaggttt tgcaatttct catttcctga aatggttatt tgcgcttctc
     3481 catttgttgg gtttggtgct atttgaattt gtaatgagtt attttcaata tagcttttgt
     3541 tctcgttatc atttttatca caaagcacat tcaagcattg ttgcatttgc tgaattgttt
     3601 tttcaagtga tgaaactttg ttttttagtt cggagttttc tttgtcaact tcctgaaccg
     3661 ctttaaccaa actcggtaca aactgtgaat aagcaatgct gtaattatct gttgggtttg
     3721 tgggagcatt tactccatca aactgataac ctattttttt tgcagtagct tctacttctt
     3781 gtgcaataaa acctgtttga atatttttct tggctgctgc aattgcatct gcatcgggat
     3841 agtaatggcg cgccatgcta tcaggcattt gtgctgtaat gtgatgattc aatttatcca
     3901 catcaacaac ataagttacc ggacgcaacg catttataaa ttccaaaccg ggtacatttt
     3961 cttttacatc ttctttaaaa cgaccatcac ttaaattgct ccagggtttg taaccaccta
     4021 tgaccattgt agcctggcca ctttggccaa ttacaatttt ataagtggca ttagaaacag
     4081 agaaggaacc aagggcagta gcaccaacaa ttccatttgt acttacttca gcaaaatcac
     4141 ctatgccggt attggtaacg ccttcggggt gtaaatagag cgaacgatag ccacaggcag
     4201 tattgccaaa tccggtcgta ttgttgtaaa gtgcagaagt gccattggcc acgttagcaa
     4261 atcctgaagt gttatagtat agcgaattgg caccatatgc tgtgttatca tccgttgtgg
     4321 tacttgaata aagtgcatta tacccggtgg ccgtattata atttccggtg gtattgctat
     4381 aacttgcacc acttccattt gctgtattat agcttccagt agtatttttt tgcagtgaat
     4441 ttaatcctgt tgctgtattt tgatttccaa ctgtattgaa aagcagagag taggagccat
     4501 ttgccgtgtt ataattacca gtggtgtttt gataaagggt acgatagcca ttggctgtat
     4561 tcagaagtcc tgttgtattg ctataaagtg cttctgcgcc atgggcacta ttagatgcgc
     4621 cacctgtatt ggaaaatagc gcccatttcc caattgcagt attttcagtt ccataagagt
     4681 tggaggataa agccccggca ccattggcaa cattattact tcctgatgta tttgaaaaca
     4741 aagcactgtt accaatagct gtattatttg tgcctgttgt atttccttgc aaagcataag
     4801 caccataggc aacaccgcca ccaccggttg tattattgta taaggcatat actcccattg
     4861 cagtgttttc gtttcctgtt gtgttgtgat taagcgctac attaccaatg gcaatatttc
     4921 cgctgcctgt tgtattattt aataatgcgc cataacccaa ggcactattt tcatttccgg
     4981 tttgattact taccagcgca ttacttccaa tagccgtatt ttgataaccc aatgtattat
     5041 tcaaaaggct ataagtgcca acggcagtat tgtaacctcc tgttgtagtg gaacccaagg
     5101 attgataccc taaagcagta ttgttaactc ctgtattgtt gatcaaagtt ttataaccat
     5161 agcttgtgtt ggcatttgca ctgtaatcta gttttccaga tagctggttg ttaaccttaa
     5221 attgtaatgg cttagcatct tttgttccaa taaaattggt aattgaagtt cccgaattgc
     5281 catttaatcc ccacccggca gttgttttaa ttgcagtcca tgcagtgcca gtgtaataat
     5341 aaaatcccgg tgtgctgttg gtttgataaa ttaataaacc tgttgcaggt gctgcaatgg
     5401 cattgcgctg tgtagatgtc atacgcggaa tcagcattcc ctttgtagtt gattttattt
     5461 ctaacaagct tgatgcagca ggagcattgg taccaatgcc ggcactgcca tttgcaggaa
     5521 atgtgttttg agcataaacg cttatttgta caaaaagcat aattattaaa atgcctgaga
     5581 aatatttttt catttttatt tagtttttat ttttatatta atttattcaa taaccagctt
     5641 ttgcgattga atcatttcag ttttgttaaa taattgaact aaaaaagttc ctggatttaa
     5701 tgttgatgtg tttaactcga aaacagcgtt gccgataact tggtaagcat taatttgcct
     5761 tccaactgca tcaaaaatct ttagtgtata tgttttgctt ttatccgatg agtttattga
     5821 aatatttgca acataatgtg caggattagg agaaatgacc atatctattt ccgcactatt
     5881 ttgaagagcg ttcttttgat tactggccaa taaatccaat tgattttgca tatcaataat
     5941 tgcagctttt atttcacggt tttctttttt tatttcctga ttttcttttt ttaaatcctc
     6001 attctgttgg gataattcct gtattgactt aactaccggc accacaaatt ctgcataccg
     6061 aagacccata atttttccgc ttttgtcaac accactgaaa tcataattta ttttttttgc
     6121 cgaagcttcc acttcctgtg caataaatcc tgtgaaggcg attttttcga tatcatattt
     6181 ttcaggatac gaactattgt cactaatgcc tgtcaattca ttttctttat tcaaatcgaa
     6241 atgataggta acaggacgta aatcatttat aaatattaga ccggggacat tttctttaat
     6301 ctctttttta attctgccat ccgaaaaatt ggtccagcca acctgcccgc caatactgct
     6361 cacagaattg tcgcctaagc gtattttgtt ggaagcatct acgattgctt cgttgccaat
     6421 agcggtaata ttattaaagg tgccaataaa tgttgttgtg tttgctcctt ctccaacggc
     6481 agtattcctt tgtccggtgg tattccaatt tagagcgcgc tggccaactg caacgtttgc
     6541 actgccggat gtattgtatt caaatgcacc ataacctaca gctacattat gtaaacctgt
     6601 tgtattggaa gacatgcatc catagcccat agcacaattt ccactccctg tagtatttga
     6661 acgaagcgct tgggaaccaa aggcagaatt tccatcagca tttggtccgt tagaatttaa
     6721 taaagcctga gagccgcatg cagtgttatc tcttcctgtt tcattgctat ataacgattt
     6781 gaatccaaat gcagaattac tgtatccgct ggtattaaat aaaagagaac catacccata
     6841 agctgcattg gcatatcctg catcattttg tgttagtgca taagcaccgg tagcagtatt
     6901 ttgggtgccg gaagtgtttt ctgataatgc tgcaaaccct attgcagtat tttcatcagt
     6961 attattaatt cgtaatgcag agttgccaat ggcaatattg tattgacctg ttgtattata
     7021 ctgcaaggca cttttcccgg cagcaatatt attagaacct gaagtatttg aaaacagcgc
     7081 aagtgaacca actccaatat tagaacttcc tgttgtattt gcattcaatg catcactacc
     7141 aattgctata tttgtattac cggttttatt ttgcaataat gctgtattac caacagccac
     7201 attcgagcta cctgtagtat tatttaacag cgccccatat ccaaaagcag cattataatt
     7261 tccgctttgg ttgctttgta atgagttact tccaacggca gtgttttgat aaccaacagt
     7321 gttatttaac atactataag ttcctactgc tgtgttgtaa cccgctgttg tatttaattt
     7381 caaagtattg tatccaacag ctgtgttgtt ataatttgcg ttgctcatca atgcctgata
     7441 gccatagctt gtatttgccg tagtatttga agacaatata cccgctaatt gattattggt
     7501 tttcaaaata aagcttgccg tatcagtagt tccaataaaa ttaacacctg agttagtacc
     7561 tgcatttcct tttaagcccc aaccggcagt agtttttaat gctgtccatg ctgtgccggt
     7621 gtaataataa aaaccgggag tgttagtagt ttgataaatc aataaacctg ttgcaggtga
     7681 tgctatggca ttgcgttgtg aagatgtcat acgcggaatc agcattccct ttgtagttga
     7741 ttttatttct aacaag
//
LOCUS       DAORQA010000070         7740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2467_length_7740_cov_9.349772, whole genome shotgun sequence.
ACCESSION   DAORQA010000070 DAORQA010000000
VERSION     DAORQA010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2467_length_7740_cov_9.349772"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            420..>1166
                     /locus_tag="PK736_05370"
     CDS             420..>1166
                     /locus_tag="PK736_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HRC32853.1"
                     /translation="MQNKGLIRALAIILTIVCLFQLSYTWMVKSVESSAKSYGKGNVA
                     LENRYLDSMKTQKVFPPLLNVFTYKECKDRELNLGLDLKGGMNVTLELSTADVIRGLS
                     NNSTDKDFTAALNNAVTRQGNSQEGLVKLFGEEFTKINPNNKLAAIFSTADLQSKVTY
                     KSTNEEVLGVIQSEVNDAIKRSYNIIRTRIDKFGVTQPNVQQLANDRILVELPGVKEK
                     ERVRKLLQGTAELEFWPTYNLICNRNMNKAL"
     assembly_gap    1167..1176
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1177..3456
                     /gene="secD"
                     /locus_tag="PK736_05375"
     CDS             <1177..3456
                     /gene="secD"
                     /locus_tag="PK736_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRC32854.1"
                     /translation="VVGQLVEVNKTLKLINERDSLAKATASGITPSVNDTTKKSTDTL
                     ANSTDSLLNQIKNTASNDTTGKDSTGAGKQMSAEQFKREYPLFSLMTPADYMVKTQED
                     QKFVYESPRLGFAMVSDTAEINALLNRPDIRSIIKNDLQFAWTAKSSNPENDNVRELI
                     ALKVSGRDGRAPLDGSHVSEARGDRSQTNNQPEVLMQMNPEGAAIWKRMTGENKGRCI
                     AIVLDNFVYSYPKVNEVIPNGSSNISGNFTDADAADLANILKAGKLPAPCRIIEESVV
                     GPTLGEKAINDGLLSFVIAIILVMLFMVFYYNNAGWVADAALVVNLIMLFGVLTAFNA
                     VLTLPGIAGIVLTLGMAVDANVLIYERIREELRAGKGVRLAINDGYQMAYSAIIDSNV
                     TTILTGIILFIFGTGPIQGFATTLIIGIITSMFTSIFISRLIFEWMLNTNKSIKFSTK
                     TTENLFRNKNIDFIGMRNKAYIASGVVILIGLISIAVTGFNQGVDFKGGRSYRVQFNE
                     AKNAEDVRSSLATQFGNGTEVKTLENDRTFKITTPFMIESNDADADNIVEKKLTDGLK
                     INKDQIIWSQKVGPTVADDIKQEAVLVLIFGLLAIFLYIVARFRKWTFGAGAIVALAH
                     DTLIILGVFSIFKNIMPFSMDLDQAFIAAILTVMGYSINDTVVVFDRIREYLGIHKKG
                     EMSIVINKALNDTLSRTINTSLIIFFVLLAIFIFGGETIRAFSFALLLGIIVGTYSSV
                     FIATPIVIDFDKKEREIKK"
     gene            3631..4878
                     /locus_tag="PK736_05380"
     CDS             3631..4878
                     /locus_tag="PK736_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32855.1"
                     /translation="MNKKLLSAAIGCLMAGGMMAQAPVNDDCATAIDVNSLFGGALNT
                     AVVGGPYTNTNGTTANDPIDGWACYGEPDGTGTAPELNNTVWFTFTGDGNQYFIETPS
                     LNLVNGITDGDTQMSLYEGTCGALTALACNEDGPNATSTYYPAAITFFTTPGVTYYLM
                     VDGFSFNGAVSEGDFNIEVTMVPGVACGDTSVTAGTATANGTDFCEGDTVLFTGTGSV
                     YPTAGTVFGIIWVLSSADISNSTDPVNEPSYLGSTNVITSPNGTTTVQLIYNTANFPP
                     NTYYITPCVFGNGTAGGPISTLADITIDPNCFFGGTSVMFTLLDPQDPQCTGAGINEN
                     GSSASLDLFPNPVSNMLTISNKENFGIAVVTITDNLGKTIDVLNMASGQALNVNTEKY
                     PSGMYFISVSQDSKIMKGKFVKK"
     gene            4941..5228
                     /locus_tag="PK736_05385"
     CDS             4941..5228
                     /locus_tag="PK736_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRC32856.1"
                     /translation="MHLHVFLFLDSLGGGELLIIILFAIMFFGSDKLPGLMRGMGAAM
                     RTFKNAMNDVKYDIEKSVDMSEIDSTRKNLPKPGTAPIQPENTGDETPPTA"
     gene            complement(5238..>7740)
                     /locus_tag="PK736_05390"
     CDS             complement(5238..>7740)
                     /locus_tag="PK736_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC32857.1"
                     /translation="FILYSKMNSSSLQFVFSRMLMMSIVAISFSNSYSQTFYKTYGDA
                     VVADRGMVIAKATDGSMFIGGSTNDSSLIIKLNAQGELIWGKSFKHVAQGLNIVKCLQ
                     VTVDGFLIGTSLGYTNSSPIYTGSSIFKFDLNGNLIWQRTEAALIPYYFSKIIAINNA
                     EYFVAGCKFQYNAGTHADYKFFKVDASTGNITSQSSIYDFDNGGLANYYDDISAVSQI
                     DNNHVYVAGRVFAGANTNDKSRPAVAKVDLAGNIVWSKFLMKDNLASARILPTAISAL
                     NDTIIIAYHGDINGTGSSNTTGIIKMYQDGSVILTEDINIVSSGDDFCNTIAYVSDGY
                     LLCGFTNYAGISGDVFITKLNFDGTLQWTKSFGTVNKEDVFTENFESFIVDEPNNAIF
                     FTCNNSKGSSDVVVGRTDLNANLSCVTNTLEATTITTLPNFEQATTHTSINDNLSLGV
                     GVAMMSNDFPEECEDKLDLGNDTTLCLPFTFVLNANVPGGSAYLWNNGDTLPQISVAD
                     TGLYYVSVNLLCCVFTDSIYIGLTTLTVDTQFISICNGDSFVYNSHTYNSSGIYVDTI
                     YNTIACDSVIYTDLIVNNLPTISYSGDTSICEGTSVLLDFTGALNYTWIPNTGISINT
                     DSSFIVTPIAPTTYTVIGSDANNCVDSIGIPFTFKPTPVADFDFIITDNGCNYDVQFK
                     DISTGNILTYFWEFGDLQDSYDANPKHVYNGGLYNVLLYVTNTSGCTDTSLQIVTLTP
                     KQTFVFIPEAFTPNDDATNEFMEVYATCLIKVDFRIFNRWEQQIFETNKFPTKWSGEM
                     YDGTIAPAGIYGYKLEATAVDGTKFEKVGTFVLIK"
BASE COUNT         2473 a         1444 c         1488 g         2325 t
ORIGIN      
        1 gtttacacgc aaggcatgca gggcaaacaa aaaactttct ttaacaaggt tcaaaaaaat
       61 catattctct ttttcggttc acaaatgtag taatacaaat ggtgaggcaa attaatgtct
      121 ttatattaaa tacgaactgt ttgacgctac attgataatt ttgttaccat ttaaattttg
      181 ggctgattta aataaacttt cagcatcgtt tttaaaatga aattgattta tactataaaa
      241 atttcaaaaa cgagattttt gtaaagaccc gaaaccatta atttgcaggc ccatattatg
      301 actatcatca aatatttctg aacattttgg ccgatattta ttttttgtcg tttctaaaat
      361 caaaattcta tatttgcggg cttaaaaatt ttaggactta gtaaaccaat taaaaagtaa
      421 tgcaaaataa aggattaata agggctttgg cgattatact caccatcgta tgcctttttc
      481 aattatcgta cacatggatg gtaaaaagtg tagagagtag tgccaaatcg tatggcaaag
      541 gaaatgtagc tttagaaaac cgctacctcg actcaatgaa aacacaaaaa gttttccctc
      601 cgctattaaa tgttttcaca tacaaagagt gtaaagaccg tgagttgaat cttggcctcg
      661 acttgaaagg cgggatgaac gtgacactcg aactttcgac tgccgatgtt attcgtggct
      721 tatcgaacaa cagcaccgac aaagatttta ccgctgcatt aaataatgct gttacccgtc
      781 agggaaattc gcaggaaggc cttgtaaaat tatttggtga agaatttaca aaaataaacc
      841 caaacaataa gctggcagca atattcagca ctgccgattt gcaaagtaaa gttacttaca
      901 aatcgacaaa tgaggaagtt ttgggggtaa tacaatcgga ggtaaatgat gccataaagc
      961 gttcgtacaa tattattcgt acacgtattg ataagtttgg cgtaacccaa ccaaacgtgc
     1021 agcaattggc caacgaccgt attcttgttg aattgccggg cgtaaaggaa aaagagcgcg
     1081 tacgtaaatt attgcaagga acggcagaat tggagttttg gccaacctat aatttaattt
     1141 gtaatcgcaa catgaataaa gcactcnnnn nnnnnngtag taggccaatt ggttgaagta
     1201 aacaaaacat taaaattaat aaacgagcgc gattcattgg caaaagctac tgcatcgggc
     1261 attactccat ctgttaatga taccactaaa aaatcgacag atactttagc caatagtacc
     1321 gacagtttat tgaaccagat aaaaaacact gcatctaacg acactaccgg taaagatagt
     1381 accggtgcag gcaagcaaat gtcggcagag caatttaaaa gagaataccc attattcagc
     1441 ctcatgacac cggccgatta tatggtaaaa acacaggaag atcaaaagtt tgtttacgaa
     1501 agcccacgcc ttggttttgc aatggtatcg gatactgcag aaataaacgc ccttttaaac
     1561 agacccgata tccgcagtat aataaaaaac gatttgcaat ttgcatggac agcaaaatca
     1621 tctaatcccg aaaacgacaa tgtacgcgag ttgattgcat taaaagtatc aggtcgcgat
     1681 ggcagagcac cacttgatgg ttcgcatgtt tcggaagcac gtggcgaccg tagccaaacc
     1741 aacaatcagc ccgaagtatt gatgcaaatg aaccccgaag gtgctgcaat atggaaacgt
     1801 atgactggcg aaaacaaagg tcgttgcatt gcaatagtac ttgacaattt tgtgtattca
     1861 tatccaaaag taaacgaagt aataccaaac ggaagcagta acatcagtgg taattttacc
     1921 gatgccgatg ctgccgactt agcgaatatt cttaaagccg gtaaactgcc cgcaccctgc
     1981 cgcatcattg aagaatcggt ggtagggcct actcttggag agaaagctat caacgatggt
     2041 ttgctgtcat tcgtgattgc tattattctt gtaatgttgt ttatggtatt ctattacaac
     2101 aatgccggtt gggttgccga tgcagctttg gttgtaaact taataatgct gtttggtgta
     2161 ctcactgcct ttaatgctgt gttgacactg ccaggtatag caggtattgt gcttacatta
     2221 ggtatggctg tagatgcaaa cgtacttata tatgaacgta tacgtgaaga gttacgagcc
     2281 ggcaaaggtg tacgcctggc cataaatgat ggttaccaaa tggcctactc agcaatcatc
     2341 gactccaacg ttacaacaat acttacaggt attatcctct tcatatttgg aacaggacca
     2401 atacaaggtt ttgcaacaac gcttatcatt ggtattatca catcaatgtt tacttctatc
     2461 ttcatatcgc gcttgatatt tgaatggatg ttaaatacaa acaaatcgat aaaattctcg
     2521 actaaaacta cggagaatct tttccgaaat aagaacattg attttattgg aatgcgtaat
     2581 aaggcctata tagcttcagg ggttgtaatt cttattggtt taatatctat tgcagttaca
     2641 ggtttcaatc aaggggtcga tttcaaaggt ggacgttcat accgtgtaca atttaacgaa
     2701 gctaaaaatg ccgaagatgt acgctcatca ttggccacac aatttggcaa tggtaccgaa
     2761 gtgaaaacat tggaaaacga tcgcaccttc aaaattacta cgcctttcat gatagaaagt
     2821 aatgatgccg atgctgataa tatagtagag aaaaagctaa ctgatgggct taagataaac
     2881 aaagaccaaa ttatttggtc gcaaaaagta gggccaacag ttgccgatga tataaagcaa
     2941 gaggctgttt tagttttaat tttcggcctt ttggcaatat tcctctatat tgttgctcga
     3001 ttcagaaaat ggacctttgg cgcaggcgct attgtcgctc tggcacatga tacattgatt
     3061 atacttggcg tgttttcgat ttttaaaaac atcatgccat tttcaatgga cctggatcag
     3121 gcgtttatag ctgcgattct tactgtaatg ggatattcga taaatgatac cgtggtggta
     3181 ttcgaccgta tacgtgagta tttgggcata cataaaaaag gagaaatgtc gattgtgatt
     3241 aataaggctt tgaatgatac tcttagccgt actatcaaca catccctcat aatctttttt
     3301 gtattgttgg ccatattcat atttggtggc gaaaccattc gtgcattctc ttttgcattg
     3361 ttgttaggta ttatagttgg aacttattct tctgtattta ttgccacgcc aatagttatt
     3421 gacttcgaca aaaaagaaag agaaattaag aaataatttt tcaaaatgat ttattaaaaa
     3481 agaccggttt agtccggtct ttttttttga ttatgatttt tgtttggcgc actcatgcaa
     3541 agtgaaattt aaaaatcttc ttaaataagt tcttggtaga gttcgaattt tccactactt
     3601 tcgtgatatt aaaatttata aaaaaacaag atgaacaaaa aattactaag tgcagctata
     3661 ggctgcttga tggcaggtgg aatgatggca caagcccctg ttaatgatga ttgtgccaca
     3721 gcaattgatg tcaattcatt gtttggtgga gcgctcaata cagcagttgt tggtgggcca
     3781 tatacaaaca ccaatggtac aacagcaaat gatcctattg atggatgggc ttgctatggc
     3841 gaacctgatg gcacaggaac tgctccggaa ttaaacaaca ccgtatggtt tacatttaca
     3901 ggtgatggta accaatattt tattgaaaca ccatcgctaa atttagtgaa tggcattacc
     3961 gatggtgaca cacaaatgtc gctttacgaa ggcacttgcg gtgcattaac tgcattggct
     4021 tgcaacgaag atggcccaaa tgcaacaagc acatattatc ctgcagctat tacttttttc
     4081 actacaccag gagttaccta ttaccttatg gtagatggtt ttagctttaa cggtgctgtg
     4141 agcgaaggtg atttcaatat tgaagtaaca atggttcccg gtgtggcttg tggcgacact
     4201 tctgtaactg caggtacagc aacagcaaac ggcacagatt tttgtgaggg cgatacagta
     4261 ttgttcacag gaaccggatc ggtttatcct acagcaggaa cagtttttgg tattatttgg
     4321 gtactttcaa gtgcagacat aagcaatagc accgatcctg taaacgagcc atcctacctg
     4381 ggttctacca atgtaatcac atcgccaaat ggaacaacaa cagttcagtt gatttacaac
     4441 acagctaact ttcctccaaa tacctattac attactccat gtgtttttgg taatggtaca
     4501 gcgggcggtc ctatctctac tttggccgac attactatcg atccaaattg cttttttggt
     4561 ggcacttcgg ttatgtttac cttactagat cctcaagatc ctcaatgcac aggtgccggt
     4621 ataaacgaaa atggttctag tgcaagttta gatttgttcc caaaccctgt tagcaatatg
     4681 cttactattt cgaacaaaga aaactttgga atagctgttg ttaccattac agataattta
     4741 ggaaaaacta tcgatgtatt gaacatggca tcgggccagg cattaaatgt aaatacagaa
     4801 aaatatcctt caggaatgta tttcatatca gtctctcaag attcgaaaat aatgaaaggt
     4861 aaattcgtaa aaaaataatt gcttttgaac taaaagaaaa ctgctccaaa cggggcagtt
     4921 tttttttgta cttttgtgcc atgcatttac atgtatttct ttttcttgat agtttgggag
     4981 gtggcgaatt gcttatcatc atactgtttg ccatcatgtt ttttggcagc gataaacttc
     5041 ccggtcttat gcgtggaatg ggtgctgcca tgcgtacatt taaaaatgcc atgaatgatg
     5101 tgaaatacga tattgagaaa agtgtggata tgtcggaaat tgatagtacc cgaaaaaatt
     5161 tacctaagcc cggcaccgct cccattcagc ccgaaaacac tggagatgaa actccgccta
     5221 cagcataatt ttttacttta ttttatcaaa acaaaagtgc caactttttc aaacttggtg
     5281 ccatcaactg cagtagcctc aagcttgtaa ccataaattc cggcaggggc aatggtacca
     5341 tcatacattt ccccactcca tttggttgga aatttattgg tttcaaaaat ttgctgttcc
     5401 catctattga aaatacgaaa atctactttt atcagacacg ttgcatacac ttccatgaac
     5461 tcgtttgtgg catcgtcatt tggtgtaaat gcttccggaa taaaaacaaa tgtttgcttg
     5521 ggggtaagcg ttacaatttg caaacttgtg tcggtacaac ctgatgtatt tgttacgtat
     5581 aacaatacat tgtacaaccc tccattgtat acatgcttag gattggcatc gtaagagtct
     5641 tgcaaatcgc caaattccca gaagtacgtg agtatatttc cggttgaaat atccttaaac
     5701 tgaacatcgt aattacagcc gttatctgta ataataaaat caaaatcggc aacaggtgtt
     5761 ggcttgaatg tgaaaggtat tcctattgaa tcaacacaat tattggcatc gctacctata
     5821 actgtatacg tagttggagc tattggtgtt acaatgaatg aactatcggt gttaatggaa
     5881 atgcccgtat tgggaatcca tgtataattt aatgcaccgg taaaatctaa taatacagaa
     5941 gtgccttcac aaatactggt atcgcccgaa tacgaaatcg taggtagatt atttactatc
     6001 aaatctgtat aaattacaga atcgcaggca atggtgttat aaatggtatc aacataaatg
     6061 ccggatgagt tataagtatg actgttgtaa acaaaactat caccgttgca tatcgaaata
     6121 aactgtgtat cgaccgttaa ggttgttagg ccaatataaa tactatcagt aaatacacaa
     6181 cataacaaat ttaccgacac gtagtataaa ccagtatcag ctacagaaat ctgaggtaag
     6241 gtatcaccgt tattccacaa atatgcagac ccaccgggca cgttagcatt cagtacaaaa
     6301 gtaaacggca ggcatagagt agtatcgttg cccaaatcga gtttatcttc acactcttca
     6361 ggaaaatcgt tactcatcat tgcaactcca acacccagac ttaggttatc gttaatgctt
     6421 gtgtgcgttg ttgcctgttc aaagttaggc agtgtagtta tggttgttgc ttctaatgta
     6481 ttggtgacac aagaaagatt agcattcaaa tctgttcggc ctacaaccac atcactgctg
     6541 cctttcgaat tgttacaggt aaagaaaatt gcattgttgg gttcatccac aataaaagat
     6601 tcgaaatttt ctgtaaaaac gtcttcctta tttactgtac caaaagattt tgtccattgc
     6661 aatgttccat caaagttcaa tttggtaatg aatacatcac cgcttatacc tgcataattt
     6721 gtaaatccgc acaacagata accatccgaa acataagcaa tggtgttgca aaaatcatcg
     6781 cccgaagaaa caatgttaat gtcttcggtt aaaataactg aaccgtcttg atacatttta
     6841 attattcctg tggtgttact tgaaccggtg ccattaatat cgccatgata agcaataatg
     6901 attgtatcgt ttaaagcaga aattgcagtg ggcaatattc ttgccgatgc caggttatct
     6961 ttcattaaaa attttgacca aactatgttg ccagctaaat ctacttttgc aacagcaggg
     7021 cgcgatttat catttgtatt tgcaccagca aatactcgtc ctgcaacata tacatgattg
     7081 ttatctattt gcgaaacggc cgaaatatca tcgtagtaat tggcaagtcc gccattatca
     7141 aaatcataaa tggaagattg tgatgttatg tttcctgttg acgcatcaac tttaaaaaat
     7201 ttgtaatcgg catgtgtacc ggcattgtat tgaaatttac aacctgccac aaaatattcg
     7261 gcattgttga tggcaattat cttcgaaaaa taatatggta ttagtgcggc ttcggttcgt
     7321 tgccagatta aatttccatt caggtcgaat ttaaaaatgg atgacccggt ataaattggt
     7381 gagctattag tatatcccag tgaggtgcca atcaaaaaac catcgacagt tacttgcaaa
     7441 cattttacta tgttcaatcc ctgagcaaca tgtttgaaag attttcccca aatcagttca
     7501 ccctgtgcat ttaacttgat aattaaagaa ctatcattag tactaccgcc tataaacatc
     7561 gaaccatctg tagcttttgc tattaccatt cctctgtcgg caactacagc atcgccataa
     7621 gttttataaa atgtttggct atacgaattg ctgaatgaaa tcgcaacaat ggacatcatc
     7681 aacatgcgcg aaaaaacaaa ttgtaaactt gatgaattca ttttactata gaggatgaac
//
LOCUS       DAORQA010000071         7682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2492_length_7682_cov_9.988462, whole genome shotgun sequence.
ACCESSION   DAORQA010000071 DAORQA010000000
VERSION     DAORQA010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2492_length_7682_cov_9.988462"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1556
                     /gene="preA"
                     /locus_tag="PK736_05395"
     CDS             204..1556
                     /gene="preA"
                     /locus_tag="PK736_05395"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HRC32858.1"
                     /translation="MANLQTNFAGIKSPNPFWLASAPPTNSGYQIMKAFDAGWGGAVW
                     KTLGVPVVNVSSRYGSLNYRDTRMMGFNNIELITDRPLKDNLREIEEVKKRFPNHAVI
                     ASLMVETKEEWHQIMRDVTNAGADGVELNFGCPHGMCERGMGSAVGQEPKILKMIVEW
                     VKEVATIPVIVKLTPNISDITRPAVAAVEGGGDAISLINTIQSIIGVDIDNFVPYPIV
                     DGKSTNGGYCGPAVKPIAMNMLKNCAQHSQVKIPISGIGGVENWRDAVEFILLGASSV
                     QVCTAVMHYGFGIIREMILGLEQYMDEKGFKTLDDFRGKALPNIVHWEDLNMKYKVVA
                     DINEDKCIGCQLCYTACEDGAHQAIGLSDDLLNRKPHIIEENCVGCNLCSVVCPVEDC
                     ITMQRKDDGKENITWKERTLAGDIPKTFNDELAGGLHHWVPEPVDALSKKIPLHYKVK
                     "
     gene            complement(1712..2272)
                     /gene="ruvC"
                     /locus_tag="PK736_05400"
     CDS             complement(1712..2272)
                     /gene="ruvC"
                     /locus_tag="PK736_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRC32859.1"
                     /translation="MIKDQVILGIDPGTVVMGYGLIAVNGKTTRMITAGVMQLEKYSD
                     HAVRLKKIFEKVSALIIEYHPDELAIEAPFFGKNVQSMLKLGRAQGVAIAAALHHQLP
                     VIEYSPRKIKMAVTGKGGASKEQVAAMLKQTMDINLTPKFLDATDALAVAVCHSLQNK
                     KLQSTSKSYTGWKAFITDNPDKMAGK"
     gene            complement(2429..3193)
                     /locus_tag="PK736_05405"
     CDS             complement(2429..3193)
                     /locus_tag="PK736_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRC32860.1"
                     /translation="MSQGSVNTAIENNIATITFEHPQSNSLPGAILQQLADAITSVGK
                     NADAKVIVLQSAGEKTFCAGASFDELISINDFETGKKFFSGFANVINAMRQAPKFVIA
                     RLQGKAVGGGVGLACAADYTLATSSSQVKLSELAVGIGPFVVGPAVERKIGTAAFCEL
                     AINATEWRDAAWAQQKGMYTQVFETIAQMDTELQALSKRLSQSNPEAMMMLKKIFWQN
                     TDHWNTLLIERAAMSGQLVLSDFTRNAIAKFKGGAR"
     gene            complement(3231..3626)
                     /locus_tag="PK736_05410"
     CDS             complement(3231..3626)
                     /locus_tag="PK736_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32861.1"
                     /translation="MKNFNSKISFILIIACMLFACNKDTFTPVEVTYLVLVKPGNTVT
                     INCYNDYYFDTEKLKPILHTGEGLYFRSSHIAYKEEDYYINVAYTDSTKSAEDNYLVW
                     VKFNDSTVTKLSYKYAKPNIEIKGRVTEL"
     gene            complement(3800..6904)
                     /locus_tag="PK736_05415"
     CDS             complement(3800..6904)
                     /locus_tag="PK736_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRC32862.1"
                     /translation="MSYKKINNIVGWVVFAIASYVYLTTMEPTGSFWDCGEFIACCNG
                     LQVGHPPGAPMFMLMGRMFILFAGENVKQIPLAINALSAICSALCILFLFWSITHLAR
                     KMVVKGKNEPNMGDIIAIMGSGFVGAIAYTFTDSFWFSAVEGEVYATSAMFTAVVFWA
                     MLKWEEVADEKHNARWIIFIAFMMGLSIGVHLLNLLAIPALALIYYFKRYKFTWVGFV
                     AALGVSIAILGFVQKGIISYFIKIGAQFDLVFVNSFGLPFWSGFMFYLLVTLGIIAYA
                     LWYTTRKGKVVLNTALLAVTFILIGYSSYSVIVIRSTANPPLDENDPENVFSLLNYLG
                     REQYGDFPLAYGQYYNAKTIDSDFKDMQYVKKDGKYVEVGRKESPVYDPAFCGIFPRM
                     WSPQDNHASFYKNYHKGNAEERPTSGENISFFIDYQVMHMYFRYFMWNFVGRQNDIQS
                     YSDNITKGNWISGITPIDNMRLGPQEKLPQSMTNNKGRNKFYFLPLILGLVGFAYHVQ
                     KNRKDFVIVGMLFFMTGMAIVLYVNQPPYQPRERDYAYAGSFYAFSMWIGLGVLAIFD
                     LLRNKLSLPTAGAAIAATVVSCSVPYVLCADGWNDHNRALRYTSRDFATNYLNSCAPN
                     AIIFTNGDNDTFPLWYAQQVEGVRRDIRIVNLSLLNTDWYVNQMKRQEYNSPPVPFTL
                     TYDKYMQGTRDYVPFVEQKSLSANYTELKQVFNFMTSENPETKVQVRDGKYINYLPTK
                     KLRITVDKAAVIKSGTVDIKDTAKIVDYIDWDINKSYVMKADLMMLDLLAHNDWKRPV
                     YYAVTVGSDNYIGLDDYFQLEGLAYRFVPIKTKNADQQTGRVATDLMYKNMMDKFVWG
                     NMKNEKVYLDQNNLNMTMNFRNNFARLSESLLNEGKSTQSVAVLDKCNEEMPDKTVPY
                     NMMMLRITEMYYRNAMQAATSVDSTGRPIIDPEKMSQSQKGILEKGNSILRRLAVIYE
                     DDLNYYFSLRGTKYYAFIEKDMNQSMAIMNEMVRLSKLTNQPAIIKEMEDAYGNVMKK
                     YQGN"
BASE COUNT         2288 a         1501 c         1486 g         2407 t
ORIGIN      
        1 gcacaatata tttccactta tattggttat ttctttttta taagaagcga tttgataata
       61 tttaaaggca caacttgtta gttgaaatag gtgcaaggtt tttataatgg taaccaaata
      121 aattttggta aaagtatatc gttgttaaaa atattgtact tttcggaggt gttacaattt
      181 taataataat gcaatagatt atcatggcaa atcttcaaac aaatttcgcg ggaattaaat
      241 cgccaaatcc tttttggttg gctagtgcgc caccaacaaa tagcggttat cagatcatga
      301 aagcattcga tgccgggtgg ggtggtgctg tatggaaaac actgggcgtg ccggttgtaa
      361 acgtatcgtc acgttatggt tcactcaatt atcgcgacac gcgaatgatg ggcttcaaca
      421 atattgaatt gataactgat aggccactta aagataattt gcgtgagata gaagaagtaa
      481 aaaaacggtt tcccaatcat gcggtgatag catcgttgat ggtggaaaca aaagaagagt
      541 ggcatcagat aatgcgcgat gttaccaatg ccggtgccga tggtgtggag ttaaattttg
      601 gttgcccaca tggtatgtgc gaacgcggaa tgggaagtgc agtagggcag gaaccaaaga
      661 ttttgaaaat gattgttgag tgggtaaaag aagttgctac cattccggtg atagtaaaac
      721 ttactccaaa tatttcggac atcacccgcc ctgcagttgc agcggtagaa ggtggtggcg
      781 atgccatttc attgatcaat accattcaaa gtatcatagg agttgatatc gataattttg
      841 tgccttatcc tattgttgat ggcaaaagta ctaacggtgg ttattgtggt cctgctgtaa
      901 aaccaattgc aatgaacatg ctgaagaatt gtgctcaaca ttcacaagta aaaattccta
      961 tcagcggaat tggtggtgta gaaaattggc gcgatgcagt agaatttatt ttactcggtg
     1021 catcatcggt gcaagtgtgt actgcggtaa tgcattatgg ttttggaatt attcgcgaaa
     1081 tgattttagg attagagcag tacatggatg aaaaaggttt taaaaccctt gacgattttc
     1141 gtggtaaagc attgccaaat attgtgcatt gggaagacct caatatgaag tataaagtgg
     1201 tggcagatat taacgaagac aaatgcattg gttgtcaatt gtgttacact gcatgcgaag
     1261 atggtgcgca tcaggctatt ggattgagtg atgatttgct aaaccgtaag ccacacatca
     1321 tagaagaaaa ttgtgttgga tgtaatttgt gttctgtggt atgtccagtt gaagattgta
     1381 taaccatgca acgcaaagat gacggcaaag aaaatatcac atggaaagaa cgcacacttg
     1441 caggtgacat accaaaaaca tttaacgatg aacttgccgg tggattacat cattgggtgc
     1501 ccgagcctgt agatgcactt tcaaaaaaaa taccattgca ttataaagtg aaataaaatt
     1561 tggttgattg gttgttgatt attatttttt taaatcccaa aattaattcg aatgtgtttt
     1621 tgcattgaaa atatttgcga aaatattcta cacattttgg tttgctcagt aatttattat
     1681 caattcacaa atgactattc aaacaaaaaa attatttgcc cgccatctta tcaggattat
     1741 cggtaatgaa tgctttccaa ccggtgtatg attttgaagt tgattgtagt tttttgtttt
     1801 gtaacgaatg gcaaacagca acagcaagtg catcggtagc atctaaaaat ttgggagtaa
     1861 gattaatgtc cattgtttgt ttcagcatgg cagcaacttg ttctttagaa gcgccaccct
     1921 tgccggttac agccattttt attttgcgtg gcgaatattc tatcacgggt aactgatgat
     1981 gcagtgccgc agcaattgct acaccctgtg cacggccaag ctttagcatg ctttgtacat
     2041 tcttgccaaa aaagggcgct tctattgcaa gctcatcggg atgatattcg ataatgagtg
     2101 ccgatacttt ttcgaatatt ttttttaacc gtacagcatg gtcgctgtat ttttcaagtt
     2161 gcattacacc ggcagttatc atacgtgtag tttttccgtt tacagcaata aggccgtaac
     2221 ccattacaac agtgccgggg tcaatgccga gtataacctg atcttttatc aatgtgaaat
     2281 gtataagctg caaatataga agggcattgt cgatgaactg atggtgcaaa aatattttat
     2341 caactatcag cgctttatta aaattgtttg aatagatggc tcacattaca agcgcaaaaa
     2401 aaattttata aaaaataatt ttgtaaaact acctcgctcc gcctttaaat tttgcaatgg
     2461 cattgcgcgt aaaatcggaa agcacaagct gaccactcat agcagcccgt tcaattaaaa
     2521 gtgtattcca gtgatcggta ttttgccaga atattttttt cagcatcatc attgcttcgg
     2581 ggttcgattg cgataatctt tttgacaatg cctgtaattc tgtatccatt tgtgctatgg
     2641 tttcgaatac ctgcgtatac attccttttt gttgcgccca tgctgcatcg cgccattcgg
     2701 ttgcatttat ggcaagttcg caaaatgccg cagttcctat ttttcgctct acggcagggc
     2761 ctactacaaa cgggccaata cctaccgcca actcgctaag tttcacttgc gatgatgatg
     2821 ttgctaaagt gtaatcggca gcacatgcca aacctacacc accacctact gctttacctt
     2881 gtaaacgtgc aatcacaaat tttggcgctt gccgcatagc atttataaca ttggcaaatc
     2941 ccgaaaagaa tttttttcct gtttcgaaat cgtttatcga tatcaactca tcgaacgatg
     3001 caccggcaca aaatgttttt tcccctgcac tttgcagtac aataactttt gcatcggcat
     3061 ttttgccgac acttgttatt gcatcggcaa gttgctgcaa aattgcgccc ggcaggctat
     3121 tgctttgtgg gtgctcaaac gtgatggtgg ctatgttatt ttcgatagcg gtatttaccg
     3181 atccttgcga catgtgtaca gttttatttt gtgattatta tattttccag ttacaactcc
     3241 gtaacgcggc cttttatttc gatgttgggt ttggcatatt tgtacgatag ttttgtaact
     3301 gtagagtcat taaattttac ccacactaag taattgtctt cagcgctttt tgtagagtcg
     3361 gtatatgcca cattgatata ataatcttct tctttgtaag caatgtgcga tgaacgaaag
     3421 tataaaccct caccggtatg caaaatgggc ttcagttttt ctgtatcgaa ataataatcg
     3481 ttgtagcagt taatggtaac agtattgccg ggctttacca ataccagata tgtaacttct
     3541 accggagtga atgtgtcttt gttgcaggca aaaagcatac aggcaataat cagaatgaat
     3601 gatattttcg aattaaaatt tttcattgtt taatacttac gtaattgaac aaacgttttt
     3661 ttacatcata aattatatac tgctgcacca caaaaaatca gctacattgt tacaatagca
     3721 agcgtatatg gtatatgttg ttttccacat tttgtaaaaa atatttttat aaaaatgcgt
     3781 ttcaaaaaaa tcaagtaaat taattaccct gatatttttt cattacatta ccatatgcat
     3841 cttccatttc tttaataatg gccggctgat ttgtaagttt cgacaaccgc accatttcgt
     3901 tcattatggc catcgattgg ttcatatcct tttcaataaa agcataatac tttgtaccgc
     3961 gcaaagagaa atagtagttc aaatcgtctt cgtatatcac cgcaagtctg cgcaaaattg
     4021 agttgccttt ttctaaaatt cctttttgcg attggctcat cttttcggga tcgatgattg
     4081 gccttccggt tgaatcaaca gaagttgcag cctgcatggc attgcggtaa tacatttcgg
     4141 ttatgcgcaa catcatcata ttgtaaggca ctgttttgtc gggcatttct tcgttacact
     4201 tatcaagcac agcaaccgat tgggtcgact taccttcgtt caataacgac tccgacaagc
     4261 gcgcaaaatt attgcggaag ttcatggtca tgttaagatt attttgatcg aggtacactt
     4321 tttcgttttt catattgccc cacacaaatt tatccatcat gtttttgtac ataaggtcgg
     4381 tagctacacg gccggtttgt tggtcagcat tttttgtttt tattggcaca aaacgataag
     4441 ccaaaccttc gagttggaaa taatcgtcaa ggccaatata attgtcacta ccaactgtaa
     4501 cggcatagta cacggggcgt ttccaatcgt tgtgtgcaag caagtccagc atcataaggt
     4561 cagccttcat cacataactt ttatttatat cccaatctat ataatcaaca atttttgctg
     4621 tgtcttttat atccaccgtt ccgcttttta ttacagcagc tttgtcaaca gtgatgcgca
     4681 atttttttgt gggcaaatag ttgatgtatt ttccatcgcg cacctgcact ttggtttcgg
     4741 gattttcgct tgtcataaaa ttaaacacct gtttcaactc ggtgtaattt gccgacaaac
     4801 ttttttgttc tacaaatgga acataatcgc gtgtgccttg catgtactta tcgtatgtaa
     4861 gtgtaaatgg caccggtggc gaattgtatt cctgacgttt catttggttt acataccagt
     4921 ctgtatttaa taaactaagg tttacaatcc gtatatcgcg ccttacccct tctacctgtt
     4981 gtgcatacca caatgggaaa gtatcgttat cgccattggt aaatatgatg gcattgggtg
     5041 cacacgagtt aagataattg gtggcaaaat cgcgcgatgt ataacgtaat gcacggttgt
     5101 gatcgttcca tccatcggca cacaatacat atggtaccga acacgaaact accgttgctg
     5161 caatggccgc accagcagta ggaaggctta acttattgcg caacaaatcg aaaatggcca
     5221 acacacccaa tcctatccac atcgaaaaag catagaacga tcctgcataa gcataatcac
     5281 gctcgcgcgg ttgatagggt ggttgattta cataaagtac aatggccatt cctgtcataa
     5341 aaaacaacat gcctacaatg acaaaatctt tacggttttt ttgtacatga tatgcaaagc
     5401 ccaccaatcc taaaattaaa ggcaagaaat aaaatttgtt gcgcccttta ttattagtca
     5461 tgctttgcgg caatttttct tgcgggccaa ggcgcatatt atctattgga gtaataccgc
     5521 taatccaatt gcctttggta atgttgtcgc tataactttg tatatcgttt tggcggccaa
     5581 caaaattcca cataaaataa cggaagtaca tgtgcattac ctgatagtcg ataaagaaag
     5641 aaatattttc gccacttgta ggtcgttctt cagcattgcc tttatgatag tttttgtaaa
     5701 aacttgcatg attatcttgt gggctccaca tacgtggaaa gataccacaa aaagccggat
     5761 cgtaaacagg cgattccttg cgacctactt ctacatattt accgtctttt tttacatact
     5821 gcatatcctt aaaatccgag tctatcgttt ttgcattata atattgcccg tatgctaaag
     5881 gaaagtcgcc atattgctcg cgacccaaat aatttaaaag tgaaaataca ttttccggat
     5941 cgttttcatc caatggagga ttggccgtgc tgcgtataac tatcacagaa taggatgagt
     6001 atcctataag aataaatgta acagcgagca aggcagtatt caataccact ttgcctttgc
     6061 gcgttgtata ccacagtgca taagcaataa tgccaagtgt aacaagcagg taaaacataa
     6121 agcccgacca aaatggtaag ccaaaactat ttacaaatac taaatcgaac tgtgcaccaa
     6181 tttttataaa ataggatatg atgccttttt gtacaaaacc taaaatggca atagaaactc
     6241 ccagtgcagc aacaaagcct acccatgtaa atttgtaacg cttaaaataa taaatgagcg
     6301 ccaatgctgg tatggctagc aagttgagta aatggacacc aatagaaaga cccatcataa
     6361 aggcgataaa aattatccat cgtgcattgt gtttttcatc ggccacttcc tcccacttca
     6421 acatagccca aaacactact gcggtaaaca ttgccgaagt tgcatacacc tcgccctcca
     6481 cggccgaaaa ccaaaacgaa tcggtaaaag tataagcaat agcgcctaca aaaccacttc
     6541 ccattatggc aattatatcg cccatattag gttcgttttt accctttacc accattttac
     6601 gtgcaaggtg tgtaatgctc caaaacaaaa ataatataca cagcgcacta catattgccg
     6661 acaaagcatt tatagctagt ggtatttgtt ttacattttc gccagcaaat aaaataaaca
     6721 tacggcccat caacataaac attggcgcac cgggaggatg acctacctgt aagccattac
     6781 aacaggctat aaattcgcca caatcccaaa agctgcccgt aggctccata gtagttagat
     6841 atacgtagga ggcaattgca aacacaaccc aaccaacaat gttgtttatt ttcttataac
     6901 tcataattat ttataatata gataatgcta agtgggcgaa ggtaaaggaa attattctac
     6961 tgccaaacgt taattgattg tgaatgggga ggggagggtg ttgaggtatt tggctgttgg
     7021 tttttgcttg tcttcggttg tcttcgcagc cacccgttta tttctcaaaa acattacaaa
     7081 gcaattcctt gtcctcggtt ggcgtccctg ccaatcgctt acatctcaaa aatatttcaa
     7141 aacaattttt aaactctact atgaatgatt tccgattttg tcctcggttg gcgtccccgc
     7201 cacccgctta ttttgcaaaa atatataaca gcaattttta tattctgctt tgaatgattt
     7261 ctttgtcctc ggttggcgtc cccgccaccc gctaatatca caaaaatatt ttgtcctcgg
     7321 ttggcgtccc cgcgacccgc atattttgca taaatatata acagcaattt ttatattctg
     7381 cattgaatga tttctttgtc ctcggttggc gtccccgcca cccgctaata tcacgaaaat
     7441 atttcaaaac aatttttata ttctgctttg aatgatttct gattttttaa tatccacgaa
     7501 attaatttga taagaagtat accacaacta gaatagttta taatttagtg attgattcaa
     7561 atagtttatg cagcaagacg ggtggcgggg acgccaaccg aggacagaaa gtaaatcacg
     7621 acaagtacag tttgttattt agaaattgat tcaaataatt tatccagcat ggcgggtggc
     7681 gg
//
LOCUS       DAORQA010000072         7501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2555_length_7501_cov_7.713000, whole genome shotgun sequence.
ACCESSION   DAORQA010000072 DAORQA010000000
VERSION     DAORQA010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7501
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2555_length_7501_cov_7.713000"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1007)
                     /locus_tag="PK736_05420"
                     /pseudo
     CDS             complement(26..1007)
                     /locus_tag="PK736_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201273.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            1192..1830
                     /locus_tag="PK736_05425"
     CDS             1192..1830
                     /locus_tag="PK736_05425"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRC32863.1"
                     /translation="MTDTFRHQGMRKKLCDELRAKGIVSTDVLQAINAIPRHMFMDKA
                     FLEFAYQDKAFPIGAGQTISQPYTVAYQTQMLEIKRGEKVLEIGTGSGFQTCVLCKMG
                     ARVYSVERQRELYDYTPKLIEALGYSPKLFYGDGFKGEPMFAPYDKIIVTCGAPNIPK
                     ALIDQLKPGGRMIIPVGMDDQIMTLVVKDMDGKITSSEFEKFRFVPMLPDKS"
     gene            2119..3756
                     /locus_tag="PK736_05430"
     CDS             2119..3756
                     /locus_tag="PK736_05430"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRC32864.1"
                     /translation="MIARFNAWFLVVPLIVVLLGWNITQNKADAVYYNGTVYTVDSTF
                     STCEAFAVKNGKVVWTGKSREALDFVSPVKVDLRGQFVYPGFIDAHCHFYGYGVDQKK
                     IDLTGTSSFRAVLDTLTLHKTKLFSGWLFGRGWDQNDWANKTYPNKSELDKLFPNVPV
                     FLLRVDGHAALCNSLALQQCNVNMETKIEGGEIEKVAGELTGILIDNAVDLVKLKIPL
                     PDKKAETQALLTAQKNCNEVGLTTVCDAGLSKEVILLIDSLQQSSILKMRYYAMIEYD
                     AKNAKYFFDNGKIKTERLNACSFKMYADGALGSRGACMRDAYHDRQGHHGFLLHSVAE
                     LQQAANEIALHNFQMNTHCIGDSANHLMLQIYANALKGKKDARWRIEHAQVLASEDIG
                     WFAKYGIIPSVQPTHATSDMYWAHDRVGAERMKAAYAYKQLLDVCGMIAAGSDFPVES
                     INPLFGFYAAVARKDQKGYPKNGFQKQDAISRQDALRAMTIWAAYAAFEEKEKGSIEA
                     GKFADFVITSQDIMKHPENDLFNIKVKQTFINGERVF"
     assembly_gap    4081..4117
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4237..4764
                     /locus_tag="PK736_05435"
     CDS             4237..4764
                     /locus_tag="PK736_05435"
                     /inference="COORDINATES: protein motif:HMM:NF025391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4241 domain-containing protein"
                     /protein_id="HRC32865.1"
                     /translation="MGFFDKLFGKKKEETKPFVYADYNVVFDNKIIDGKEISIVEIGM
                     LNISSGKIVACDPLVNPESVPLARTVVAGKYPVNIFVAKNEDGEKLNALAKLEFSKNR
                     SVKWEPAVSDDDDLLTLKGDGDYIGINVDAGVAGFFDYDAGILYNKFIDDFIEVHPDG
                     NIYTDVFAAEFKRVP"
     gene            4776..4958
                     /locus_tag="PK736_05440"
     CDS             4776..4958
                     /locus_tag="PK736_05440"
                     /inference="COORDINATES: protein motif:HMM:NF025391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4241 domain-containing protein"
                     /protein_id="HRC32866.1"
                     /translation="MHELGSWVNFQLPKSELNITMFTSGEGDGLYPIYWGIDKDGNPV
                     SLIIDFNVLDLTSELD"
     gene            5455..6867
                     /locus_tag="PK736_05445"
     CDS             5455..6867
                     /locus_tag="PK736_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32867.1"
                     /translation="MMRKIILFELNEVPWRVVKYYCDKNPDSTFAKILPQCFKYETVT
                     PDQGHLSPWITWPTLHRGVDNTKHGIGDLNQDLEQVNQDYPPIWQILQKNGIKTGVFG
                     SMHSSPVPSDFRKYVFYVPDAFAVDKTAHPEYIEPFQDFNLSMSRESARNVSGGINMK
                     TGSQMLMKVPQLGFKMNTFFDLGKQLLDERKRPWVRTRRRTYQVVLAFDVFMKQMRKS
                     KPQFVTFFSNHVASSMHRYWAATFPGDYKQFDIGKDWVDTYKNEIDFTMNKFDKFFSE
                     MVAFANENPDYKIVVASSMGQASTVAELLDSQLLMGNEAKFFEFLKMDASQWKHVPSM
                     FPQYNVTIIPEKVEYFANQLKRISIAGRPLEFRQMENGFFAVDLGQKNLKEKVIQFDG
                     KDYSFEETGFENMEIDDHSGSTAYHIPEGTLVIYDPQDKSIKKEITQIPSTALVPSIV
                     KNFDIPMPAYLKYQPIAGIN"
     gene            complement(7034..7294)
                     /locus_tag="PK736_05450"
     CDS             complement(7034..7294)
                     /locus_tag="PK736_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32868.1"
                     /translation="MEASLSVSVFVNPAAKILNVVISTKNNYTANLYTTNEKGQIQNT
                     ITKRLNAGTNNFAFNIASLTPGLYFVAVRTKVGISKTKWIKL"
     gene            complement(7306..>7501)
                     /locus_tag="PK736_05455"
     CDS             complement(7306..>7501)
                     /locus_tag="PK736_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32869.1"
                     /translation="GGDGWQIIRHDFSRTTPDIYIKATIANDYVLQNDVNAALILGHL
                     AVAHLHRQMELAPLSTLPQM"
BASE COUNT         2225 a         1251 c         1625 g         2363 t
ORIGIN      
        1 atttgttgtt attgcctgat aggttttaca catttatagt ttgcagtcgg tcgataattt
       61 gttgtgctgt ttctaatacg cgcacaccat cggcaaggct tactacgggt gttgtgttgt
      121 tgtttatgct gtgggcaaac agggtgagtt cgtgctttat ggcgtttact tcttgtatgg
      181 gcggatgttc tacgcttatg aggcggctgc ctttatcgtt tggcaagtct atcaccattg
      241 cgaatgggtc gttgaggtct gcgggtgcgg gttgtatttt tattatctca catttttttt
      301 tcgaggaagt cgacactgat gtaagcctcg ctttgaaaga agcgcgactt gcgcatattt
      361 ttcatggata tacggctggc ggtaaggttg cacacacagc catttgcaaa ttcgatacgg
      421 gcatttgcta tgtcgggggt attgcttaca atggctacac cgttggcata tatttttttt
      481 acgggcgatt ttacggtgtg tagtacaatg tcgatatcgt gtatcataag gtcgagtaca
      541 actgatacat cggtgccacg cgggttgaat tgtgccaggc ggtgtgtttc gataaacatg
      601 ggattgatga tgctgctttg cacggccatg aatgcctgat tgaaacgctc tacatgacct
      661 acctgtactt ttacatttgc ttcgtcaata atttttatga gttcgcgtgc ttctttcact
      721 gtggcggtta caggtttttc gataaagatg tggcggccgg ccatggcagc gcgtactgca
      781 cactcatgat ggtatacagt aggggttaca atgtcgataa cattgcaggc ctgtatgaga
      841 tcgctttcgt gttcgaaaaa cggaatgccg gtttcggtac tatattgttg tgctttggca
      901 gtatcggtat cgaacacgcc tacgaggttg tattcgggaa tttcgcgcag caactttgcg
      961 tgtattttgc ccaggtggcc aatgcctatt actccaactt ttaacataaa ctattttggc
     1021 acgaaataaa atatcttaat tcaattaaaa agaaaaaagt gaaggagttt tacagtcaaa
     1081 ttggttgaaa ctttgtgcag tattgcagtg taaattaaaa taaaggggct tatcattgtg
     1141 gccgcaatat ttaaaaaaaa agatagtggc aatttactca tggcaataaa aataacggat
     1201 acattcaggc atcagggcat gcgtaaaaaa ttgtgcgatg agttgcgcgc taaaggtatt
     1261 gtgagtactg atgtattgca ggccataaac gctataccgc ggcatatgtt tatggataag
     1321 gcctttttgg agtttgccta tcaggacaag gctttcccta ttggtgcggg gcaaacgatt
     1381 tcgcaacctt atacagtggc gtaccaaacc caaatgctgg aaataaaacg tggcgaaaag
     1441 gtgttggaaa ttggtaccgg ttcgggtttt caaacatgtg tgctatgtaa gatgggtgca
     1501 cgggtttatt cggtagagcg gcagcgtgag ttatacgatt atacacctaa actgatagag
     1561 gcattgggct acagcccgaa gttgttttat ggcgatggat ttaaaggcga acctatgttt
     1621 gcgccttacg ataaaataat agtaacgtgt ggcgcgccca atattcctaa agcattgata
     1681 gaccaactga aacccggagg caggatgatt attcccgtag gaatggatga tcaaataatg
     1741 actttggtag ttaaagatat ggatggcaaa attacttcga gcgaatttga gaagtttcgg
     1801 tttgtgccga tgttgccgga taagagttag tttaaagttg aaagttgata gtttaaagtt
     1861 gaatgcaaat gcggttttag ttttatgttc ttagtttttt agtcccgaca ctgcggtcgg
     1921 gatgccgacc gaagtgtcgg cattcttggt tttttgtttt tgtctaagta ctttggttgt
     1981 gttaatggtt ttagtttctt gaactttaaa ctgaaaactt caaactttaa actcaaaact
     2041 tcaaacttca aactataacg gaatagtaat tgtataggag tgcacgttaa gaaatagatt
     2101 tttataaaga tgaaatgttt gatagcgcgt tttaacgcat ggtttttagt ggtgccatta
     2161 atcgttgttt tattgggttg gaacattacc caaaataaag ccgatgctgt ttattacaat
     2221 ggaactgtat atacggtaga tagcaccttc tcgacatgcg aagcttttgc tgtgaaaaat
     2281 ggtaaagtag tatggacagg caaaagtcgt gaagctttag actttgtaag tcccgtaaaa
     2341 gtagaccttc gcgggcagtt tgtgtatccg ggttttattg atgctcactg ccatttttat
     2401 ggctatggtg tcgatcaaaa gaagatagac cttaccggca cctcatcttt tcgggctgtg
     2461 ctcgatacac ttacactgca taaaacaaaa ttattttcgg gttggttgtt tggtcgtggg
     2521 tgggatcaaa atgactgggc aaataaaaca tatcccaata aaagtgagct tgataaattg
     2581 tttcccaatg tgcctgtttt tttattacgg gtagatggac atgcggcatt gtgcaactcg
     2641 ctggcattgc agcaatgtaa tgtgaatatg gaaacaaaaa ttgaaggcgg agagattgaa
     2701 aaagtagccg gtgaacttac cggaatatta attgacaatg ctgtggacct tgtaaaatta
     2761 aaaattccat tgcccgataa aaaagctgaa acacaagcat tgctgacagc acaaaaaaat
     2821 tgcaacgaag tcggccttac cacagtgtgc gatgccggcc tgagtaaaga agttattttg
     2881 ttgatcgatt cgttgcaaca aagcagcata cttaaaatgc gctactacgc catgatagag
     2941 tatgatgcaa agaatgcgaa atattttttc gacaatggta aaataaaaac agaacgtctg
     3001 aatgcatgct catttaaaat gtatgccgat ggtgcgttgg gttcgcgtgg tgcatgtatg
     3061 cgcgatgcct atcacgatcg tcagggtcat catggttttt tattacactc cgtagccgag
     3121 ttgcaacaag cggccaatga aatagcatta cacaatttcc aaatgaacac gcattgcatt
     3181 ggcgattcgg ccaatcatct tatgttgcag atttatgcca atgcacttaa aggaaaaaaa
     3241 gatgcgcgct ggcgcataga acatgcacag gtgctggcat cagaagatat tggatggttt
     3301 gcgaagtatg gtattattcc atcggtgcaa cctacacatg ccaccagcga tatgtattgg
     3361 gcgcatgata gagtaggggc ggagcgtatg aaagcagcat atgcctataa acaattgctc
     3421 gatgtgtgtg gtatgattgc tgccggcagc gattttccgg tagaaagtat taatccattg
     3481 tttggttttt atgctgctgt ggcacgcaaa gaccaaaagg gatatcccaa aaatggtttt
     3541 caaaaacaag atgctatttc gcggcaagat gctttgcgcg ctatgacaat ttgggcggcc
     3601 tatgcagctt tcgaagaaaa agaaaaaggt tcgatagagg ctggcaagtt tgccgatttt
     3661 gtaattacca gtcaggatat aatgaagcat cccgaaaatg atttgttcaa cataaaagtg
     3721 aaacaaacat ttatcaatgg cgagcgtgtt ttttagtttg ctatcgttta aataaataca
     3781 caaaagcact cgacttattt ttttgcttgg gttctaaatt tttcggaaaa aatgtttccc
     3841 gcagataccg ctgataaatt aaatgccgca gattttcgca aatctaattc attgttggtg
     3901 cacaaaatca gagagaatgc attgtgctga ataagattga atttacttta attttttttt
     3961 cggttccgaa agtacttact gccagctttt ctattcaatt cgaaaatact gttccaaaaa
     4021 ttttctataa aattaaaaat ggctagcttg tatcaataaa tttttagcat ggtattcttt
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta ttctttttat tatatcatca
     4141 caatttttcc gttttggttt aggagtgaat tgaattattc tttattgatg gcgaataatt
     4201 atttttacca ttgcaaaaac ataaacaaaa aaaagtatgg ggttcttcga taaattattc
     4261 ggcaagaaaa aggaagaaac aaaaccattt gtgtatgccg attacaatgt ggtgttcgat
     4321 aataaaataa ttgatggtaa agaaatatcg atagtagaaa ttggcatgct gaatattagc
     4381 agcggaaaaa ttgtagcatg cgatccgctt gttaaccctg agtcggtgcc attagcacga
     4441 acagtagttg caggcaagta tcctgtaaat atttttgtgg caaaaaatga agatggagaa
     4501 aaattaaatg cattggccaa actggaattt tcgaaaaaca gaagtgtgaa atgggaacct
     4561 gcggtgagtg atgatgacga tttgttaacg ttgaaaggcg atggcgatta tattggtatt
     4621 aatgtggatg caggtgtggc aggatttttt gattacgatg ccggcatatt gtataataaa
     4681 tttattgatg attttataga agtgcatccc gatggaaata tttatactga tgtttttgca
     4741 gctgaattta aaagagtgcc gtagataaaa acaatattca tgaattaggt tcgtgggtca
     4801 attttcaatt gccaaaaagc gaattgaata ttaccatgtt tacatcgggc gaaggcgatg
     4861 gtttgtatcc tatctattgg ggtattgata aagatggtaa cccggtatca ctcataatcg
     4921 attttaatgt gcttgatttg acttctgagt tagattgata ttttttgcaa gttctttttc
     4981 gcgcatcttt tttttgaaaa atattttgtg ataaaatgca tgcccatatt tttatttgaa
     5041 gaaaacagtg attgctttta tgaatcatat gattgaaaat tgaagggcga tattgaacca
     5101 taaacctgaa agattttttt attttttttt gacaaattga aatgctgaaa ttcatttttg
     5161 gaaaacactg taagaaaaat attatggcaa accttatata ttttgatgat taagtttgaa
     5221 tatttcgggg cataactaat tgtgaagtaa acggccatta attatgattg acaaatcttc
     5281 tttaatattg ttgaaaacaa tgatgtggct gcatgcactt gggcaacggc atttggttaa
     5341 tcattaaaga tttcatttat ttgcgcatat aaatatcaac actacttaat aaattatcac
     5401 aattgctttt tagttttggt aaagaataaa atcaggaaat agctaacaga aaaaatgatg
     5461 agaaaaataa tattgttcga acttaacgag gtaccttggc gcgtagtaaa atactattgt
     5521 gacaaaaacc ccgatagtac ttttgcgaaa atacttcctc aatgttttaa atacgaaaca
     5581 gtaactcccg accaggggca tctgtcacca tggattacct ggcctacttt gcaccgtggt
     5641 gtggataata cgaagcatgg tattggcgat ttgaatcagg accttgaaca agtgaatcag
     5701 gactatccgc ctatatggca aatactgcaa aaaaatggta ttaaaaccgg agtgtttggc
     5761 tccatgcatt catcacctgt tccatccgat tttcgtaaat atgtttttta tgtgcccgat
     5821 gcatttgctg ttgataaaac agcacatccc gaatatatag aaccatttca ggattttaat
     5881 ttatcgatgt cgcgcgaatc ggcacgcaat gtaagtggtg gtatcaatat gaaaaccggt
     5941 agtcaaatgc tgatgaaagt gccgcagctt ggttttaaaa tgaatacttt tttcgacctt
     6001 ggaaagcaat tgctcgatga acgtaaacgc ccgtgggtac gcacccgcag acgcacgtat
     6061 caggtggttt tggccttcga tgtatttatg aaacagatgc gaaaaagcaa acctcagttt
     6121 gttacgttct ttagcaatca tgttgcatcg tctatgcatc gctattgggc ggcaactttt
     6181 ccgggcgatt acaaacaatt cgacattggc aaagactggg ttgataccta taaaaacgaa
     6241 atagatttca ccatgaataa gttcgataaa ttctttagtg aaatggtggc gtttgcaaat
     6301 gaaaaccccg attataaaat tgtggtagca agtagtatgg gccaggcttc aactgttgcc
     6361 gaattgctcg actcacaatt gcttatgggt aacgaagcga aatttttcga gttcctgaaa
     6421 atggatgcgt cacaatggaa gcatgtacct tctatgttcc cacagtataa cgttaccatc
     6481 atacccgaaa aggtagaata ttttgccaat caactgaagc gtatttccat tgccggacga
     6541 ccgctcgaat tcagacaaat ggaaaatgga ttttttgctg tcgatttagg acaaaaaaat
     6601 cttaaagaaa aagtaataca atttgacggt aaagattatt cgtttgaaga aacaggtttc
     6661 gaaaatatgg agatagatga tcattctggt tcgactgcct atcatattcc cgaaggaaca
     6721 ttagtgattt acgatccgca ggataagagc attaaaaaag aaatcacaca aattccttct
     6781 accgctttgg ttccatctat tgtaaaaaac ttcgatatac ctatgcctgc atatctgaaa
     6841 tatcagccta ttgccggaat caattaatgc ataaatttta attgcaatat gtatgttccc
     6901 gcgcttataa gtgctacttt ttattgcgca taaaatttaa attctgtttc gaattattgg
     6961 aaaaaaatat tgttgcgctg ccctattctt ggctcatctt atcttcaggc atttgttgat
     7021 catgattttg caattacaat tttatccact tggttttaga tatacccact ttggttctta
     7081 cagcaacaaa gtataaaccc ggagttaatg aagcaatgtt aaaagcaaaa ttatttgtac
     7141 ccgcatttaa tcgtttagtg attgtgtttt gtatttgtcc cttttcattt gtggtataca
     7201 aatttgccgt gtaattattt tttgtgctta taaccacgtt taaaattttt gctgccgggt
     7261 ttacaaatac acttacactt aaagatgctt ccatctccat taggtttaca tttgtggcaa
     7321 tgtcgaaagt ggtgccaatt ccatttgccg atgcaaatgt gccacagcca ggtgccctaa
     7381 tattaatgca gcgttaacat cattctgcaa tacataatca tttgcaattg tcgccttgat
     7441 gtatatgtcg ggtgttgtgc gcgaaaaatc gtgtcgtata atttgccaac catcgccacc
     7501 t
//
LOCUS       DAORQA010000073         7397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2589_length_7397_cov_12.905748, whole genome shotgun sequence.
ACCESSION   DAORQA010000073 DAORQA010000000
VERSION     DAORQA010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2589_length_7397_cov_12.905748"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..1093)
                     /gene="atpG"
                     /locus_tag="PK736_05460"
     CDS             complement(212..1093)
                     /gene="atpG"
                     /locus_tag="PK736_05460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRC32870.1"
                     /translation="MANLKEIRIRIASVMSTQQITKAMKMVSAAKLRRAQDAIVQMRP
                     YAGKLREIIENVSSSLDSSSGGAFAAQRKVEKVLIVAITSNRGLCGGFNSNIIKGVNK
                     RINEEFAPQAKAGNVKVLSIGKKASESFSKKSNMSVGDYNTVYAALTFDNVAPIAEKI
                     MADFAAGNYDRVEIVYNLFKNAAVQYVTTEQLLPITPPSKNEKSKAVNDYIFEPSKEQ
                     IVTDLIPRSIKTQLYKALLDSHASEHGARMTAMSKATDNAQELLRDLRINYNKARQAS
                     ITNEILEIVAGAEALNG"
     gene            1231..1929
                     /gene="tsaB"
                     /locus_tag="PK736_05465"
     CDS             1231..1929
                     /gene="tsaB"
                     /locus_tag="PK736_05465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRC32871.1"
                     /translation="MSYILLIETATETCCIALALKDKVVAQRLVTKQKAHAGVINVFI
                     KEVLQDAGIDFDKLDAIAVSSGPGSYTGLRIGVSTAKGLCFAIDKPLIAIDTLLSYAH
                     QYYIENKSSLIHPQNTLFIPMIDARRMEVYTAVYNQNFETIVKVNALVLQEDSFLEYE
                     NKFDLHFFGDGAPKIKSFLKEKASIIVKENFIPNAAGMSVLAHNKLLNQEYENLAYFE
                     PFYLKDFVTQSKFR"
     gene            2087..7249
                     /locus_tag="PK736_05470"
     CDS             2087..7249
                     /locus_tag="PK736_05470"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRC32872.1"
                     /translation="MKKALLLSLFGFSLINCTRLSAQLNVNSSVTATQLVNTILGGGV
                     TISNVVYTGTAPGKGTFTSPNTNLGMISGITLSSGDAVQSGSALGTFASENTNGNGDP
                     DLDIIVSPKVTYDAAVLEFDFSVASDSVQFRYVFGSEEYNDYVNTAFNDVFGFFISGP
                     GIVGIKNIAVIPNTTTAVSINNVNNGGPYPGLSSGPCTNCNYFVDNINGPSIYLDAFT
                     TVLTAKSAVQPCETYHIKLAIADVSDHVFNSAVFIEGGSFSSLGQINLFANGVAVANN
                     DTVFGCVGSQVTLNLNSASNYNWSTGATTQSIVVNSPPLGVVNQYSAFVSNPNFSCFA
                     FTTTVYVATLVPTAVITPNGSLSLCPGGNVALVANTGNNYLWSNGATTQSVLVNGAGT
                     YTVTVTNLGGCSAVSVPVSVTIGNAVANITGPAALCNGAAANLQANVGQAYIWSTGAT
                     TQNISAAIAGVYTVTVTQAGGCTATASVNLNINPNPIPAITGVSTICQGGLASLNAGA
                     GFSNYLWSNAATMQTIAPAASGTYTVTVTDANGCTGTTSVLMTINPNPTPTITGPTNI
                     CAGSSANLNAGAGFNGYVWSNGVTTQIQNFNLPGTYTVTVTDANGCSGTTTTVLNVVS
                     NPTPSISGLSTVCSGAAANIDAGGGYLSYLWSNGNSTQVINPTTSGTYTVTVAISGGC
                     TGTASVIVTVNNNPTPSITGINTICQGSGTSFNGGAGYSNYSWSNGSTTQTININSSG
                     TYTVTVTDANGCSGTSSNTLTVNANPAPSITGTASVCQGASSILNGGGGYLSYNWNGG
                     STNQTISANATGNYTVTVTDNNGCTGTASFAFTVNALPNPTITGVCSICSGSAATLNG
                     GGGYIVYAWSDGSTSQVINPTTSGIYTVTVTDANGCSNTASINVTVNANPVPVVTGIN
                     TICQGANTTFNGGAGYSQYNWSNGNSTQTINVGTSGTYTVTVTDINGCTGTASYSLTV
                     NPNPSTVITGTMQFCQGLNSVIDGGAGFSSYVWNNGSTTQNITISNGGTYTVTVTNNF
                     GCTNTATTQITVNALPVPSITGSASICQGANSLLDAGAGYTLYAWSNGNTTQQLNTVN
                     SGTFTVTVTNANGCTGTASFTVVVNANPTPVIVGNTVICQGTPTTFNVGNGYATYVWN
                     TGANSASINPTATGTYTVTVTDNNGCTATATLPLTVNPLPQPSISGVNSICQGNNTVF
                     DAGAGFVQYAWSNGSSTQTINLNSSGTFTVTVTDNNGCTNSASYTLVVNALPTPTITG
                     VPSICQGTSTVLDPGSYVSYQWNNGNANQTLNVNSTGTYTVTVTDINGCSGTASFSVV
                     ANALPVPVITGINSICDGATTTLSTGSFSSYVWSNNSANSTLTTGVAGNYVVTVTDAN
                     GCTGSSSPFTLTVLYATSTIAANGPLHFCAGGNVSLSANSGSSYLWTGGATTQTINVN
                     SSGTYIVTVINTNGCTATSAPVVVGKHEYPIVKFLYDSAVICGALRVQFFNQSQADPG
                     STIKWQFGDGGTSTIPAPVHDYGKPGTYTIILTVTSPWGCSSTDSTSILVDYPADPIA
                     KFTCKPNIATFLNPQIQFTDLSQHAVAWYWNFDDGEKSEEQNPLHFFKEAKEHVISLT
                     VLNISGCQDTYKETILITPLWIPNTFTPNNDGKNDSFFTSDYIANVQSYRMVIYNRWG
                     GKVYETTEFTQPWDGLDIGGNPSPEGTYVYRLEVVTKGGKAHEFSGTVDLLR"
BASE COUNT         2220 a         1473 c         1514 g         2190 t
ORIGIN      
        1 acgttgcaga gttcgctaat tcattacatt taatttatgc cttagattaa ggccaatgga
       61 tatgatattg gaatttgttg gacataatat ggggtagccg atttttagga tgcataaagt
      121 tattcaatgc aatcattaac aaatatatat ccggttgatg ttacaacaaa aattaagtat
      181 aaaaataaaa aaggctaatt aaaaacggat tttatccgtt caatgcttcg gcaccggcaa
      241 caatttcgag aatttcgttg gtgattgaag cctggcgtgc tttattgtag ttgatgcgta
      301 aatcgcgtaa caactcctgc gcattatcgg tggctttgct catggcggtc atacgtgcac
      361 cgtgctcact ggcgtgcgaa tcgagtaatg ctttgtaaag ttgtgtctta atagaacggg
      421 gtataaggtc ggtaactatt tgctcttttg aaggctcaaa aatataatcg tttacggcct
      481 tgcttttttc gtttttcgat ggtggtgtaa ttggcaatag ttgctcggta gtaacgtatt
      541 gtacagcagc atttttaaat aggttgtata caatctctac acggtcgtaa ttacctgctg
      601 caaaatcggc cattattttt tcggcaatag gagcaacatt atcaaaagta agggcagcat
      661 atactgtatt gtaatcaccc acactcatat tgcttttttt cgaaaaactt tccgatgctt
      721 ttttaccaat gctcaacact tttacattgc cggctttggc ttgtggagca aactcttcat
      781 tgatgcgctt gttcacacct ttaataatat ttgagttgaa accaccacac aacccacgat
      841 tggaagtgat ggcaacaata agcacttttt ccactttacg ttgagcagca aaagcaccgc
      901 cactgctact atcgagcgat gaggatacat tttcgattat ctcgcgcaac ttgccggcat
      961 aaggtcgcat ttgtacaatt gcatcttgtg ctctgcgtaa ttttgctgcc gataccattt
     1021 tcatggcctt ggtaatttgt tgtgtcgaca ttaccgaggc tattcgaatt cttatttctt
     1081 tgagatttgc catgcttttt tccttttcgg ggcgcaaata tactaaagta aatatccgaa
     1141 gcaaaaatga ttgattccgt tttataaatt gaaaagtgtg aatgtttaaa tttccttttc
     1201 aatgtttcga tttgtttatt tgcaaccatc atgtcgtaca ttttattaat agaaaccgca
     1261 accgaaacct gttgcattgc ccttgcactg aaagataaag tggtggcaca acgcttggtt
     1321 accaaacaaa aggcacatgc cggagttatc aatgtattta ttaaagaggt gcttcaggat
     1381 gcaggaattg attttgacaa actggatgct atagcggtga gcagcgggcc cggctcatat
     1441 accggattac gaataggagt gagcactgcc aagggtttat gctttgccat tgataaacca
     1501 ttgattgcaa ttgacacttt actttcatat gcacatcaat attatattga aaataaatca
     1561 agtctcatcc atcctcaaaa cacattgttt atccctatga tagatgcccg cagaatggag
     1621 gtttatacag ctgtttacaa ccaaaatttt gaaacaattg tcaaggtaaa tgcattggtt
     1681 ttacaagaag atagtttttt ggaatatgaa aataaattcg acctgcattt ttttggagat
     1741 ggagcgccta aaataaaatc ttttttaaaa gaaaaggcat ctataattgt aaaagaaaat
     1801 tttatcccaa atgcagccgg tatgtctgtt ttggcgcata acaaactatt aaatcaagag
     1861 tatgaaaacc ttgcatattt cgagcccttt tacctcaaag attttgtaac gcaatcgaag
     1921 ttcagatagt taaaacacct ctttgttttt aaattttcct gccttacaac ttttatcatt
     1981 gtttttacgt cttttaatac atactttagt agcctaaaat tgttcaaact attttagtaa
     2041 ataacacaca acataaaaca aaaaaaacgc acaaaaaact atcgcaatga aaaaggcact
     2101 tttactcagt ttgtttggtt tctcattaat aaattgtacc cgactatcgg cacaattaaa
     2161 tgttaacagc agtgttaccg ctacccaact tgtaaacaca attttaggag gaggagttac
     2221 catttcgaat gttgtttata caggcacagc gcccggcaaa ggaactttta cttctcccaa
     2281 cacaaattta ggaatgataa gcggcattac actttcatcg ggtgatgctg tgcaatcggg
     2341 ttcggcttta ggtacttttg caagcgagaa tactaatgga aatggcgatc ctgatctgga
     2401 tattattgta tcgccaaagg tgacttacga tgccgcagtt ttagaatttg acttttcggt
     2461 tgcatccgac tcggttcagt ttaggtacgt gtttggcagc gaagaataca acgattatgt
     2521 taatactgct ttcaatgatg tttttgggtt tttcataagt ggacctggaa ttgttggtat
     2581 caaaaacatt gcggtaatac ccaatacaac aacagcagtt tcaattaata atgtaaacaa
     2641 tggtggcccc tatcccggat tatcatcggg gccttgcaca aattgtaatt attttgttga
     2701 taatattaat ggaccaagca tatacctcga tgcttttact acagtgctta ctgcaaaaag
     2761 cgcagtgcaa ccttgcgaaa catatcatat taaactggcc attgccgatg tgagcgatca
     2821 tgtgtttaat tcagctgtat ttatcgaagg aggaagtttt tcgagtttag gtcaaattaa
     2881 tttatttgct aatggtgtag ctgtagcaaa caacgatact gtttttggtt gtgttggtag
     2941 tcaggttacc cttaatctaa attctgccag caattataac tggagtacag gagcaacaac
     3001 acaaagcata gttgtaaact caccaccttt aggagtagtg aatcaatatt ctgcatttgt
     3061 aagtaatcca aatttttcat gctttgcttt tacaacaact gtttatgtag ccactttggt
     3121 tcctaccgcg gtaattaccc ccaatggctc gttaagttta tgcccgggcg gaaatgttgc
     3181 tttggttgca aataccggca ataattattt gtggagcaat ggtgccacta ctcaaagcgt
     3241 attagtgaat ggtgccggaa cgtatactgt tactgttaca aatcttggag gatgcagtgc
     3301 tgtatctgta ccggtatcgg taacaatagg caatgctgtt gccaacatta caggccccgc
     3361 ggctttgtgc aacggtgctg ctgccaacct tcaggcgaat gtggggcaag cttacatttg
     3421 gtctaccggg gctactacac aaaatatatc ggctgctatt gccggagttt atactgttac
     3481 ggttactcag gccggaggat gcacggccac agcaagtgta aatcttaata ttaatcctaa
     3541 tccaatacct gctattacag gtgtaagtac tatttgccag gggggattgg catcgctcaa
     3601 tgccggtgca ggatttagca attacttgtg gagtaacgca gcaacgatgc aaaccattgc
     3661 gcctgctgca tctggtacat ataccgttac cgttaccgat gcaaacggtt gcaccggaac
     3721 cacatcagta ttgatgacca ttaatccaaa tccaacccca actattacag gcccaacaaa
     3781 tatttgtgca ggaagctccg caaatttaaa tgccggtgcc ggtttcaatg gttatgtttg
     3841 gagtaatgga gtaacaacac aaatccaaaa ttttaatttg cccggaacgt ataccgttac
     3901 tgttaccgat gccaatggct gcagcggaac taccactacg gtattgaatg tggtgagtaa
     3961 tccaacccca tctatctcag ggctttcaac agtatgttca ggagccgctg ccaatatcga
     4021 tgctggcgga ggttacttaa gttatttgtg gagcaatggt aattctacac aagtaattaa
     4081 tccaactaca tcaggaacat atacagttac agttgctatt tcgggcggat gcaccggtac
     4141 ggcatcggta attgttacgg taaataataa tcctacacca tccatcacag gaataaatac
     4201 tatttgccag ggaagcggta catctttcaa cggtggtgcc ggatattcca attactcatg
     4261 gtcgaatggt agcacaacgc aaacaataaa cataaacagt tctggtacgt atacggttac
     4321 ggttaccgat gcaaacggtt gtagcggcac ttcgagcaat acacttacgg taaatgcaaa
     4381 tcctgcaccg agcataacag gcactgcatc ggtatgtcag ggcgcttcat caatattgaa
     4441 tggtggcgga ggttacttaa gctacaattg gaatggtggt agtacaaacc aaacaataag
     4501 tgcaaacgca acaggcaact atactgtaac agtaacggat aacaatggat gtacaggtac
     4561 ggcttcattt gcttttacgg taaatgcttt gccaaaccca acaataacag gggtatgttc
     4621 aatatgttcc ggtagcgcag ccactttaaa cggaggaggc ggttatattg tttatgcctg
     4681 gtccgatggc agtacttctc aggtgattaa tcctactaca agtggcattt ataccgttac
     4741 cgttacggat gcaaatggtt gcagcaatac agcaagtata aatgttacag tcaatgccaa
     4801 ccctgtaccg gttgttacag gaattaatac aatttgtcag ggcgcaaata caacattcaa
     4861 cggtggagcc ggctattcac aatataattg gtcgaatgga aactctacac aaacaattaa
     4921 tgtgggcaca tcaggtactt acacggtcac ggtaacagat ataaatggtt gcaccggcac
     4981 agcaagttat tcgttaacag ttaatccaaa tccaagcaca gtgattacag gcactatgca
     5041 attttgccag ggcttaaatt ctgtaataga tggtggtgca ggttttagtt cttatgtatg
     5101 gaacaatgga agcacaacac aaaatattac tatcagcaat ggaggaacgt atactgttac
     5161 agttactaat aattttggct gtactaacac agccactacc caaataactg taaatgcttt
     5221 acctgttcca agtattactg gcagtgcaag catttgtcag ggagcaaatt cattgcttga
     5281 cgctggtgca ggctacacct tgtatgcctg gtcaaatggc aataccactc agcaattgaa
     5341 tacagttaat tcaggaacgt ttacggttac ggttaccaat gcaaacgggt gcacaggaac
     5401 tgcaagtttt actgttgtgg taaatgcaaa ccctactccg gttatagtag gtaatactgt
     5461 aatttgtcag ggcacaccta caacctttaa cgttggcaat gggtatgcaa cttatgtttg
     5521 gaatacagga gcaaactcag catctatcaa ccctactgca acaggaacat atacggtaac
     5581 agtaacggat aacaatggat gcacagcaac agctacattg ccattgacag taaatccttt
     5641 gccacagcct agcatatcgg gtgtaaatag catttgtcag ggaaacaaca cggtatttga
     5701 tgccggtgca ggatttgtgc aatatgcatg gagtaatggc agcagcacac aaacaattaa
     5761 tctgaatagc agtggaacgt ttacagttac ggttacagat aataacggat gtacaaattc
     5821 tgcttcatac actttagttg taaatgcttt gccaacacca accattacag gagtgccgag
     5881 tatatgtcag ggaacatcaa cggtgctcga ccccggcagc tatgtatctt accaatggaa
     5941 taatggaaat gccaatcaga ctttgaacgt aaattctacc ggaacatata ccgttacagt
     6001 tacagatatc aatggatgtt cgggcacagc atccttttca gttgttgcaa atgctttgcc
     6061 ggttccggtg attactggaa tcaattcaat ttgcgatggg gctactacta ccttatctac
     6121 cggttctttt tcatcttacg tttggtctaa taattcagca aactcaacat taaccaccgg
     6181 tgttgcaggc aattatgtag taaccgttac cgatgccaat ggatgcacag gatcatcttc
     6241 tccatttacg ctgacggtgc tatatgcgac ttctacaatt gctgcaaatg gtccattgca
     6301 tttttgtgcc ggaggtaacg tatcgctttc agcaaattcc ggcagttcat atctatggac
     6361 tggtggtgca accacacaaa ccataaatgt aaattcttcc ggaacgtata tagtcactgt
     6421 tatcaatacc aatggttgca cagcaacatc ggcacctgtt gtggttggta agcatgaata
     6481 tccaattgta aaattccttt acgactcagc agttatttgc ggggcattga gggttcaatt
     6541 ttttaaccag tcgcaagccg atccgggttc tacaatcaag tggcaatttg gcgatggagg
     6601 aacatccact atacctgcac cggtgcacga ctatggtaag ccgggtactt atacaattat
     6661 tctaacggtt accagtccct ggggttgctc aagcacagac agtacgagca tattagttga
     6721 ttaccctgcc gaccctattg ctaaatttac ctgtaagcca aacatagcaa ctttcctaaa
     6781 tccacaaata cagtttactg acttatcaca gcacgctgtt gcctggtatt ggaattttga
     6841 cgatggcgaa aaatctgaag agcagaatcc gctacatttt ttcaaagagg ccaaagagca
     6901 tgttatttcc ttaacagtat taaatatttc ggggtgtcag gatacttata aagaaacaat
     6961 actgattaca cccctttgga tacctaatac tttcacacct aataatgatg gcaaaaacga
     7021 ttcgtttttt accagcgatt atattgccaa tgtgcaatcg taccgaatgg taatttataa
     7081 tagatggggt ggtaaagtgt atgaaactac agagtttact caaccatggg atggactcga
     7141 tattggtggc aatccttcac ccgaaggaac gtatgtgtac cgcctggaag tagtgactaa
     7201 aggtggcaaa gcccatgagt tttcggggac agttgatttg cttaggtaga aaaaatatcc
     7261 caaaaatatt ttctaaatta aattctaatt taggattatt taaacgtttt gttttttcaa
     7321 aaaatggaat ggcataaccc cacccatata cgtacctaag aacaaaaatt ataaaccaac
     7381 tggatgccag gtagttt
//
LOCUS       DAORQA010000074         7337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2614_length_7337_cov_8.669871, whole genome shotgun sequence.
ACCESSION   DAORQA010000074 DAORQA010000000
VERSION     DAORQA010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7337
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2614_length_7337_cov_8.669871"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..127
                     /locus_tag="PK736_05475"
     CDS             <1..127
                     /locus_tag="PK736_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011671078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HRC32873.1"
                     /translation="IWISWPKKSSKVETDIDENVIRNFALQNGLVDIKVCSVNNT"
     gene            complement(329..787)
                     /gene="coaD"
                     /locus_tag="PK736_05480"
     CDS             complement(329..787)
                     /gene="coaD"
                     /locus_tag="PK736_05480"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010603033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRC32874.1"
                     /translation="MSRIAIFPGSFDPITIGHEFIIRRSATLFDKLIVGIGKNSSKQS
                     MFDIDKRLQWIKDSFINEPNITAIAYDGLTVNFCRAQNANYIVRGLRSFTDFEYESAI
                     AQMNRKIAPEIETVFMLTSPEYDAIASNIVRDILRNGGDVQQFLASGVRI"
     gene            complement(815..1465)
                     /locus_tag="PK736_05485"
     CDS             complement(815..1465)
                     /locus_tag="PK736_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HRC32875.1"
                     /translation="MEYNTTRNKLVIPEYGRHIQRMIEQTCLIEDREMRNLHAQSIVN
                     VMGTMLPHLRDVVDFKHKLWDHLFIISNFKLDVDSPFPIPSVMTYQVKPKKLSYPTYK
                     IRYAHYGKTVEKMIAEIRLLADGPSKQKLIVDLANFMKLLYVTWNKESLDDQLIIDQL
                     RELSKGDLRLSDGFKLAEIREHQQGNGAPIKAQKRQIRPPARGAQKPPAGAQRKKY"
     gene            1626..2726
                     /locus_tag="PK736_05490"
     CDS             1626..2726
                     /locus_tag="PK736_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRC32876.1"
                     /translation="MITQQQVIDALSNVEEPDLKKDLVTLNMIKDVAVDGNNVSFTVV
                     LTTPACPLKELIRNACINAVKYYVSQEAEVTVNLTANTTRSTNADDVLLPGVKNIIAV
                     ASGKGGVGKSTVTANIAVALAQTGARVGIIDADIFGPSIPIMFGLQDERLFVEEKNGK
                     TMMIPASRFGVKVLSIGFIADTNQAIVWRGPMASRALKQLISDAEWGELDYLFVDLPP
                     GTSDIHLTLVSAVPLTGAVIVSTPQQVALADARKAIGMFQMESIHVPILGLVQNMAYF
                     TPAELPENKYYIFGKDGGEKLATELGIPFLGEIPIVQSICDGGDQGIPIVMDEDSKAA
                     IAFYTAARNTAQQIAIRNAHQPPTQRVMAAGF"
     gene            2797..2991
                     /locus_tag="PK736_05495"
     CDS             2797..2991
                     /locus_tag="PK736_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32877.1"
                     /translation="MEEEEFIYNMRSELEDKNRENMVRINELMEKGYSAERIARYLML
                     EEEDVRKIMEESGLENDELV"
     gene            complement(3298..3504)
                     /locus_tag="PK736_05500"
     CDS             complement(3298..3504)
                     /locus_tag="PK736_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRC32878.1"
                     /translation="MKPNMGTVDKAVRILVALVILGLYAANQISGLAATVLLILAGIF
                     ILTSFMSFCPLYLPFGISTRKKEK"
     gene            complement(3704..4054)
                     /locus_tag="PK736_05505"
     CDS             complement(3704..4054)
                     /locus_tag="PK736_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32879.1"
                     /translation="MKLENKKQKVVPLDRFIIRMGRYALFAFILIATSLGIGELGYHF
                     FGKLNWLDSFHMSSMILTGMGPVVEMKTNGAKIFSSIYALYSGVAFLSSTAVFFAPIV
                     HRVMHILQVEQDDS"
     gene            complement(4122..4907)
                     /gene="proC"
                     /locus_tag="PK736_05510"
     CDS             complement(4122..4907)
                     /gene="proC"
                     /locus_tag="PK736_05510"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRC32880.1"
                     /translation="MRVHIIGGGNLGSALAIGISKFTKNVDVVMTRRNLQQIQSLASD
                     VIKISSSNIFEIDKADIIILTIKPYQVDAVLQEILPHISQKIIASAVSGCSIHSLEEK
                     TNHAHHIVRIMPNIAAQFGASATCISYSESSKNVGTQVQDLFSQLGIAVTIEEKLMDG
                     ATVLGACGTAYALRYIRAAMQAGIEIGFDAQTAMAIASQTVKGAALMTLEEKVHPEQL
                     IDRVTTPQGCTIVGLNEMEHQGFSSSLIKGIKTSFHKIKDMKS"
     gene            complement(5057..5344)
                     /locus_tag="PK736_05515"
     CDS             complement(5057..5344)
                     /locus_tag="PK736_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRC32881.1"
                     /translation="MIIRIVKLTIQNDCIAIFKEKYAVAQKQIKQFEGCKELSLLADA
                     NNHNIFFTYSKWYSEQHLNNYRNSSFFVSLWSTVKPMFAAKAEAWSLLDME"
     gene            complement(5341..6144)
                     /locus_tag="PK736_05520"
     CDS             complement(5341..6144)
                     /locus_tag="PK736_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRC32882.1"
                     /translation="MEHKIVTLITDWGTRDYFAAAFKGMLFSKLPGITVVDISHDVQL
                     GDVLRASYILRNAYTAFPAGSIHVIGVDAERSKEEDLEEDLPRRILLIENAGHYFIGA
                     DSGIFGLIFDSTNQEIIELASYDNMNRAQLFELIIDTCYKIASGENIITMGRQVEKMK
                     ELYSFQSYLSENGIMGKIIYMDSAGNLITNINYRLFESIKLNRTFQIYLRKDLVITEI
                     SEWYDEVPKGEALLMVNHAGFLEIAMNGSNAHKMLAIKEGDQVRITFNP"
     gene            6356..7276
                     /locus_tag="PK736_05525"
     CDS             6356..7276
                     /locus_tag="PK736_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32883.1"
                     /translation="MTKHFFLSMAVAILFVGGCKKDKDEALDSKVEQHNRDANTYKSE
                     LDQTDNDINDAIKDIPAFGKTGGTASSPLCGCTIDSSQIAQKILFFNFDGVTPCFSPS
                     RTRSGQIKVQLTQGQYWSNVGSVLTLTFNNYKITRLSDNRSIQFNGTKTLQNINGNDW
                     LGFLFGTKTLKYRERALNIAVKFDDGSQATWNSARITEWSYVPAETKIVFNASGDTTY
                     NGVGNTDSWGTNRYGNSFVTFYNSAIVSNTYCGLWRPVSGKITHRLDRDFIITFGVDQ
                     QGNATPYACAYGFKINWEANNKTQEVILSY"
BASE COUNT         2282 a         1352 c         1334 g         2369 t
ORIGIN      
        1 gatatggatt tcgtggccca agaaatcatc aaaagtagaa acagatattg acgaaaatgt
       61 tattcgtaat tttgctttgc aaaatggttt agttgatata aaagtttgct cggttaataa
      121 tacatagtcg gcacttaagt ttgttattcc tgcaaaggat agaaagtgat attaagtctt
      181 caattacttc taagataaat tcaaaatagc atcatcgttg aaaaatttgt tattcatttt
      241 gttgaacatg atttaaggca aacaaaaaat tattgagcca gtaattaaac tcattaccaa
      301 aaaacaaaat caacctaata atcaataact aaattctaac tccacttgcc agaaactgtt
      361 gcacatcgcc cccgttgcgc agtatatcgc gcactatgtt tgatgcaatg gcatcatact
      421 ctggtgaagt aagcataaat acagtttcta tttcaggggc aattttgcgg ttcatttggg
      481 ctatagcgct ttcatactcg aaatcggtaa acgaacgtag gccacgcact atgtaatttg
      541 cattttgggc acgacaaaaa tttactgtca atccatcgta cgcaattgct gtaatgttgg
      601 gttcattgat aaacgaatct tttatccatt gcaaacgttt atcaatatcg aacatcgatt
      661 gcttactgga attttttcct atgccaacaa tcaatttatc gaacaatgtt gccgaacgtc
      721 ttattataaa ttcatgacca atggttatgg ggtcgaatga accggggaaa attgcaatgc
      781 gactcattaa aaaattaatt tgtgaaaagc ttatttaata tttttttcgt tgtgcaccgg
      841 caggtggttt ttgtgcgcct cgtgcaggtg ggcgtatctg gcgcttttgt gcttttattg
      901 gtgcaccatt tccttgttgg tgctcacgta tttctgcaag tttaaacccg tcactcagac
      961 gcaaatctcc tttcgataat tcgcgtaatt gatcgataat taattgatcg tcaagcgatt
     1021 ctttgttcca tgtcacatat aatagcttca taaaatttgc cagatcaaca atcaactttt
     1081 gtttcgatgg tccatcggca agtaagcgaa tttctgcaat cattttttct accgttttgc
     1141 cataatgtgc atagcgaatt ttataggtcg ggtaacttaa tttttttggc tttacctgat
     1201 aagtcattac cgatggaata ggaaatggcg aatcgacatc caatttaaaa ttagagataa
     1261 tgaaaaggtg atcccaaagt ttgtgtttaa aatccaccac atcgcgcaag tgtggaagca
     1321 ttgtacccat cacattcaca attgattgtg catgtaaatt gcgcatttca cggtcttcta
     1381 tcaaacacgt ttgttctatc attcgttgaa tatgtcggcc atattcggga attactaatt
     1441 tatttcttgt tgtgttgtat tccattttaa aaatttgaat atgcggcaaa tgtaaacttt
     1501 ttgttcatac tgcgttattg tatcatgggt atgtaaacgt gatattggtc aaaatgttga
     1561 gtaaattatt ttttttgtct gtttattaat gttcgaaaaa ctactttcgc gcaaaaaata
     1621 aagcaatgat aacacaacaa caggtaatag atgcgttgag caatgtagaa gaacccgatt
     1681 tgaaaaagga tcttgtaacg cttaacatga taaaggatgt tgccgttgat ggtaacaatg
     1741 taagctttac cgttgtactc accacgccag cctgcccgct gaaagaattg atccgcaatg
     1801 cctgcataaa cgcggtgaag tactatgtaa gtcaggaggc tgaagtaact gttaatctaa
     1861 ctgccaatac aacgcgcagc accaatgccg atgatgtgct gctgccggga gtaaaaaata
     1921 ttattgctgt ggcaagtggc aaaggaggag tagggaagag caccgtaaca gcaaatattg
     1981 cagtggctct tgctcaaacc ggtgcccgtg taggaataat agatgccgat atttttggcc
     2041 catccattcc aatcatgttt ggtttacagg atgagcgatt gtttgttgag gaaaaaaatg
     2101 gcaaaacaat gatgattcct gcttcacgat ttggagtaaa ggtgttatcg ataggattta
     2161 ttgccgacac caatcaggca attgtgtggc gtggccctat ggcatcgcgt gctttgaaac
     2221 aacttataag cgatgccgaa tggggcgagc tcgattacct ttttgtggat ttgcctccag
     2281 gcaccagcga tatacatctt acattggtaa gtgcagttcc acttaccgga gcggttatag
     2341 tttctacccc acaacaagtg gcacttgccg atgcacgaaa agcaataggc atgtttcaaa
     2401 tggaaagtat acacgtaccg atattaggac ttgtgcaaaa catggcatac tttactcctg
     2461 ccgagttgcc cgaaaataaa tattatattt ttggtaaaga tggtggcgaa aaattagcta
     2521 ctgaacttgg tattcctttc cttggcgaaa taccaatagt gcagagcata tgcgatggtg
     2581 gcgatcaagg cattcctatt gtaatggacg aagattcgaa agcggcaatt gcattttata
     2641 ccgcagcacg taataccgca cagcaaattg caatacgcaa tgcacatcag ccaccaacac
     2701 aacgtgtaat ggcggcaggt ttttaaattt tattttggca caatttttat caggtggttg
     2761 ttgaattagt aacaccacaa aactcaaaaa gaaaaaatgg aagaagaaga attcatttat
     2821 aatatgcgca gcgaactcga agataaaaac agagagaata tggtgcgcat aaacgaactg
     2881 atggaaaaag gatattctgc agaacgtata gctcgttacc tgatgcttga agaagaggat
     2941 gtgaggaaaa taatggaaga atctggtttg gaaaatgatg aattggttta gacttaccat
     3001 tcatacttta taattctaca gtttactttt gctgattttt tgaaattaga tccttttccc
     3061 gatcttaaaa attgaaactg ttgttaccaa taacaagcac acaaaatttg tggattcttt
     3121 ttatttaagt aatgaaataa acacaattct ctaaataatc atctttgatg aattgtttaa
     3181 gacaaacaaa ctatagagaa gattgcaaat taccgcaaca tctctaactc agaaaatgtt
     3241 atttatcact cctcatactc gtgttcaaat attattttga acaataatac ttgagtttta
     3301 tttttctttt ttccttgtcg aaataccgaa tggtaaatac aaaggacaaa agctcataaa
     3361 gctggtaaga ataaatattc ctgccaaaat caataataca gttgctgcca atcccgaaat
     3421 ttgatttgcg gcatacaatc ctaatattac taaagctact aaaattctaa ctgctttatc
     3481 aacagttccc atatttggtt tcatataaaa atattttttt tttactcagc aaatgtcagg
     3541 tatgcagatt taataaaagg tgacttttgt catataagtg gatttttttt cgaaaaaaat
     3601 aaaaagcaaa accaaatatt tttttggacg gatttttagc tatgcaaata tgtgccggaa
     3661 aatttatttc gtgtagcaac aatgaagtaa aaaaatcttt acactaagaa tcatcttgct
     3721 ccacttgcag aatatgcatt accctatgaa caatgggagc aaaaaagacg gcagtactac
     3781 ttaaaaaagc tacaccgcta tataatgcat aaatggatga gaatattttt gcgccatttg
     3841 ttttcatttc cacaacagga cccatgcctg ttaatatcat gcttgacatg tgaaaactat
     3901 cgagccaatt taatttacca aaaaaatgat aacccaactc accaatgcca agcgatgttg
     3961 ctatgagtat aaatgcaaat aaagcatatc gtcccattct aataataaaa cgatctaacg
     4021 gcactacttt ttgtttcttg ttttctaatt tcatagagga atatttttat aaagttatca
     4081 gtaaaataaa aacaagcatg gtcgaaacaa tttttttaca actaactttt catgtcttta
     4141 attttatgaa aagaagtttt tattcctttt atcagcgatg agctgaatcc ctgatgttcc
     4201 atttcgttca atccgacaat ggtgcaacct tgtggagtgg taacgcggtc tatcaattgt
     4261 tcgggatgaa ctttttcttc taaggtcata agcgcagcac cttttactgt ttgcgatgct
     4321 atggccattg cagtttgggc atcgaaacct atttctattc ctgcctgcat agctgcacgt
     4381 atgtaacgca acgcataggc tgttccgcat gcgccaagca cggtggcacc atccatcaat
     4441 ttctcttcaa tggtaacagc aatacctaat tggctaaata aatcttgcac ttgcgtgcca
     4501 acatttttgc tcgattcgct gtaagaaata catgtggccg aagcaccaaa ctgtgctgca
     4561 atatttggca ttatacgcac aatgtggtgt gcatgatttg ttttttcttc aagcgaatgt
     4621 atgctgcaac cgcttacagc cgatgcaatt attttttgtg aaatgtgtgg taatatttct
     4681 tgaagtactg catcaacctg ataaggtttt atagtaagta tgattatatc ggctttgtca
     4741 atttcgaata tattgcttga cgaaattttt ataacatcac tcgcaagcga ttgtatttgc
     4801 tgcagatttc tgcgtgtcat taccacatct acattttttg taaatttcga aattccaatt
     4861 gctaaggccg accctaaatt tcccccaccg ataatgtgta ctctcatttt tttctctttt
     4921 aagttattta cttttatttt ttactgtatt atttttcact ttttggtaaa gtgatttttc
     4981 acactttatg ttctgaatat ggtttctgac tgtttgtttt tattgctatg attggttatc
     5041 aattcattaa tttagtttac tccatatcca gcaacgacca tgcttcggcc tttgctgcaa
     5101 acattggttt caccgttgac cataacgaaa caaaaaaaga cgagttgcga taattattta
     5161 aatgttgttc actataccat ttactataag taaaaaatat gttgtggttg tttgcatcgg
     5221 caagtaaact caattcctta catccttcaa attgttttat ttgtttttgt gccacagcat
     5281 atttttcttt aaatattgca atgcaatcat tttgtatggt gagttttaca atacgtatta
     5341 tcatggatta aaagttattc gtacttgatc gccttctttt atcgccaaca ttttatgtgc
     5401 gttactgccg ttcattgcta tttcgagaaa gcccgcatga ttcaccatca atagcgcttc
     5461 gcctttgggc acttcatcgt accattcgct tatctcggta atcacaaggt ctttgcgaag
     5521 gtatatttga aacgtgcggt tgagttttat gctttcaaat aagcgataat ttatattcgt
     5581 aatcagatta ccggcactat ccatataaat tatttttccc ataattccat tttcagaaag
     5641 gtaagattga aacgaatata actccttcat tttttccact tgcctgccca tggtgatgat
     5701 attttcgccc gaagcaattt tataacatgt atcaattatt aattcaaata attgcgcacg
     5761 attcatattg tcgtatgatg cgagttcaat aatttcctga tttgttgaat cgaaaatcaa
     5821 accaaatatt ccactatcgg caccaataaa ataatgtccg gcattttcaa ttaataagat
     5881 ccttcgaggc aaatcttctt cgaggtcttc ctctttagat ctttcggcat ctacacctat
     5941 aacatgaata gagcctgccg gaaaagctgt gtatgcattt cgtaaaatgt atgatgcgcg
     6001 caacacatcg ccaagctgaa catcatgcga aatatcaacc acagtaatgc cgggcaattt
     6061 agaaaaaagc atacccttga atgccgcagc aaaataatcg cgtgtgcccc aatctgttat
     6121 taatgttact attttatgtt ccattttacc tgagccacaa aactaaaatt tgcaacttca
     6181 tattttttaa tttctttatt taagtttaaa aaagtttaca acatacattt acatgattgt
     6241 tctattcgaa agcaaaggaa ataaaatgaa taatttagat gtatgacttt ggttttaagc
     6301 ttaaatgcaa taaattcgca acttcatagt ttcataaaat ataaaactta ataatatgac
     6361 aaagcatttt tttttaagca tggccgtggc catactattt gttggaggtt gtaaaaaaga
     6421 taaagacgaa gcacttgatt caaaagtgga acaacacaac cgcgatgcca atacttataa
     6481 atcggaattg gatcaaaccg ataatgatat taatgatgcg ataaaagata ttcctgcatt
     6541 tggaaaaacg ggtggaactg cttcgagccc gctttgtggt tgcacaatcg attcgagcca
     6601 gatagcacaa aagattttat tttttaattt cgatggtgtt acgccatgct tcagcccttc
     6661 gcgcacccgc agcggacaaa taaaagtaca gctcacgcag ggccagtatt ggagcaatgt
     6721 agggtcggtg cttaccctca cttttaataa ttataaaatt acccgactta gcgataaccg
     6781 gtcgattcaa tttaatggca ccaaaacttt gcaaaacata aatggcaacg attggcttgg
     6841 atttttgttt ggcacaaaaa cattgaagta tagagaacgt gccttaaata tagctgtaaa
     6901 atttgacgat ggcagccagg caacatggaa tagcgcgcgc attacagagt ggagttatgt
     6961 gccagccgaa acaaaaatag tttttaatgc cagtggcgat actacttaca atggtgttgg
     7021 aaataccgat tcgtggggca ccaatcgtta tggaaattct tttgtgacat tttataattc
     7081 tgccattgta tcaaatacct actgtggctt gtggcgacct gtatcaggga aaattactca
     7141 ccgtctcgat cgcgatttta ttatcacctt cggtgtcgat cagcaaggca atgccacacc
     7201 ctatgcctgt gcctatggtt ttaaaataaa ttgggaagcc aacaacaaaa cgcaggaagt
     7261 tattcttagt tattaattcc gttcttagtc ccgacacttc ggtcgggatg ccgaccaaag
     7321 tgtcggcatt cttagtt
//
LOCUS       DAORQA010000075         7206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2675_length_7206_cov_9.029227, whole genome shotgun sequence.
ACCESSION   DAORQA010000075 DAORQA010000000
VERSION     DAORQA010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2675_length_7206_cov_9.029227"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1589
                     /locus_tag="PK736_05530"
     CDS             <1..1589
                     /locus_tag="PK736_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRC32884.1"
                     /translation="ITLLYVLSSIAFITLLIACINFINLSTARSLKRAKEVGMRKMLG
                     AGKKQLVMQFISESMVTSTVAFLFSLIMMFLLMPLMCELTASDLQMHQLFTDPILMLS
                     FVAILLFTGLIAGSYPAFFVTRFAPLQTIRGGFNSTKSTGGKIRKALVVLQFTASIAL
                     IIGAMIAYRQLNYMQNKPLGFEKEYIVTAPLYSQNINNIFTKPDSMFYQRLHTFSDAL
                     KQNPNIATVTLSDQTLGSGAQMRGIVPEGFKQEDNKFISCIAVDFGFLETYGLKVLAG
                     RDFSEKFPADKKSSFIITQEGAKSFGWKTPQDAIGKTVDREGKQGTIIGVVNDFHSNS
                     LLQPMDGVLFDITIPQLNVFSIKINAQNIPQTLSYIESKWAAFFPEKVLQYSFLDANI
                     GLQYVAQQRLSKIIFYFAALAIFISCLGLYGLIMLVTQQRIKEIGIRKVLGASVASIT
                     NLLSRDFILLITLSFLLATPLAYYAMNKWLQDFAYRTEISWWIFVIAGVAALLIAMAT
                     VSAHTIKAALTNPVKNLRTE"
     gene            1602..4022
                     /locus_tag="PK736_05535"
     CDS             1602..4022
                     /locus_tag="PK736_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC32885.1"
                     /translation="MFRNYLMIALRNLKKNKLHATINIVGLAVAFICGILLFVFVQHE
                     FSYDTFHNDNNKLYKTYNYIHDKDGESLSPTMGYPQSKMLKTEVPAVEYVSSYMNSGG
                     SVMYNDKEYEMPVYLVDTDFFSMFSFPLVECDTKNPLRDLGNVIITKKAAKIIFNQVD
                     PIGKSLSVNVNGKWQTFVIAAIAETPPRNSTLNFDVVLRIENRSDFAETKDEWGRQHH
                     PIYAKLRSNFTQTQAEKQIQSAMRKYVEADVQGLKNEGAKPDSKGDVYTIRLLPMLDV
                     HFNSEVGNQNGISKTFLYILVLIGLFVFAIACFNFINLNVARAFTRAREIGVRKCLGA
                     GAKQIFVQLVSESVILTLFALILGIVGVVIVVTHFTEIFANRFDLNILYHPFTIIFIV
                     ITSLLISIVTGGYPALLMAKFSTTETLKGKVSLKKPGAFRNSLIVIQFAIASLLICCT
                     MIIYYQFDYLRNASLGFKQENIISIPVYDVMNGRDILSKFRTRLANNPTIENVSGSSI
                     NIGIGNDKSQSKWQCGFDFNGQLVNTTMVNVAQDYFKTLGIKIIHGREFTSEYMSDTL
                     GSVIVNESMAKQLGKGNVVGTKFLMDSADGLNIVVGVVPDVHVYSMHDLTEPTTYIAN
                     TTSPFRYIFVKVNSENPKQSLDMIASIYKTIEPGKEFKGSFMDENTQRWYEQEKNLAN
                     IFFVAADIAIILSCLGLFAIALIIIEQRTKEIGVRKVLGASVFGITTLLSKEFLKLVG
                     ISIIIALPIAYFAMNKWLQDFPYRISLHWWLFVLSAGFAVIIALATVSFHSIKAALAK
                     PIKSLRTE"
     gene            4312..4695
                     /locus_tag="PK736_05540"
     CDS             4312..4695
                     /locus_tag="PK736_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRC32886.1"
                     /translation="MTPTEQEEAIGKNIITSCIKIHRALGPGLLEKVYEVCLSYELRK
                     LGHEVLRQIDIPIKYDDIIFDEGLRLDLLVDNLVIIELKAIDIVNPVWEAQVISHLKL
                     TGKNLGYLINFNVPLMKAGIRRFIN"
     gene            4908..5573
                     /locus_tag="PK736_05545"
     CDS             4908..5573
                     /locus_tag="PK736_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC32887.1"
                     /translation="MIQLKNISRWYKTSGNKSMVLNDVSLHINNGEFVSIMGPSGSGK
                     TTLLQILGMLDTADDGQYFFEDQNVFTLKEKQRSLLAKNNIGFVFQAYHLIDELTVYE
                     NIETPLIYKDVKSDERKSLVADMLDKFNIVGKKDLFPSQLSGGQQQLVGIARALISKP
                     KVILADEPTGNLNSKQGEEIMQLFSQLNKEDGVTIIQVTHSEKNAEYGNRIVRLIDGE
                     VSN"
     gene            complement(5994..6770)
                     /gene="paaG"
                     /locus_tag="PK736_05550"
     CDS             complement(5994..6770)
                     /gene="paaG"
                     /locus_tag="PK736_05550"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HRC32888.1"
                     /translation="MSSILFSIKDGVGIITLNRPDVLNSFNKEMALAMQHALDTCAND
                     NSIRCIFITGAGRAFCAGQDLAEAIAADGPALQSIVKDHYNPIIERIRTIEKPIVCGV
                     NGVAAGAGANIALACDITIAATNASFIQAFSKIGLIPDSGGTFTLPRLIGMQRATAYM
                     MLGDKISAEQAQQIGMIYKVCVAETCADEAMAIATQLATMPTLALGLTKRALNASLTN
                     NLQQQLALEEQLQTTAGKSHDYNEGVNAFLEKRKPEFIGN"
     gene            complement(6783..>7206)
                     /locus_tag="PK736_05555"
     CDS             complement(6783..>7206)
                     /locus_tag="PK736_05555"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HRC32889.1"
                     /translation="FVHKLDHEKLLTIGDNYLLFEISYINPPDNLRDVIFKIQTNGYK
                     PILAHPERYPFYYKHFDEYKRIRDSGVLLQINFNSLAGYYGIEAKKIAEQLIDEKMVD
                     LIGSDMHHSRHAHCMRKVCSEKYFYKVAEMNLLNKTLY"
BASE COUNT         2308 a         1261 c         1413 g         2224 t
ORIGIN      
        1 gtataacatt gttgtatgtg ttatcgagca ttgcatttat cactttgctt atcgcatgta
       61 taaattttat aaatctttcc acagcacgtt cactcaagcg cgcaaaagaa gtaggaatgc
      121 gcaaaatgct tggtgccgga aaaaaacaat tggttatgca attcatcagc gagtcgatgg
      181 tgacaagcac agtggcattt ttattttcgc tcattatgat gtttctattg atgcctttaa
      241 tgtgcgaact tactgcaagc gatttacaaa tgcatcaact atttaccgac ccgattctaa
      301 tgctgagttt tgttgcaatt ttattattca cgggattgat tgcaggaagt tatccggcat
      361 tttttgtaac acgctttgca ccattacaaa ctattcgtgg tgggttcaac agtacaaaat
      421 ctaccggagg caaaattcgc aaagcgttgg tggtattgca gtttacagct tccattgcgc
      481 ttatcattgg tgctatgatt gcttatcgcc agttgaacta tatgcaaaat aagccacttg
      541 gtttcgagaa agagtacatt gtaacagcgc cattatacag tcagaatatt aataatattt
      601 ttaccaagcc cgatagtatg ttttatcaaa gactgcatac ttttagcgat gcattaaaac
      661 aaaatccgaa catcgctacg gttacattat cggaccaaac gcttggtagt ggagcacaaa
      721 tgcgtggaat agtccccgaa ggttttaagc aagaagacaa taaatttatt tcgtgcattg
      781 ctgtcgactt tggtttctta gaaacgtatg gccttaaagt tttagctggt cgcgattttt
      841 cagaaaaatt tcctgccgat aaaaaatcat catttatcat tacacaagag ggtgcaaaat
      901 cttttggatg gaaaacaccg caggatgcca taggcaaaac agttgataga gaaggtaaac
      961 aaggaactat tattggagtg gtaaacgatt ttcattccaa ctcgctttta caaccaatgg
     1021 atggagtact gtttgatatc accattccac agttgaatgt tttttctata aaaataaatg
     1081 cacaaaatat tcctcaaact ctttcataca tcgaaagcaa atgggcggca ttttttcctg
     1141 aaaaagttct tcagtattct tttcttgatg cgaatattgg attgcaatat gtggcgcaac
     1201 aacggttgag taaaatcatt ttttactttg ctgcattggc aatatttatt tcgtgcctcg
     1261 gcttgtatgg ccttataatg ttggtgactc aacaacgcat taaagaaatt ggcattcgca
     1321 aagtattggg cgcatcggtt gcaagtatta caaatttatt gtcgcgcgat tttattttgc
     1381 ttatcacttt atcattttta ctcgcaactc cactggctta ttatgcaatg aataaatggc
     1441 tgcaagattt tgcttaccgt accgaaataa gttggtggat atttgtaatt gccggtgttg
     1501 cagcattgct aattgccatg gcaacggtaa gtgcgcacac cataaaagct gcattaacca
     1561 atcctgtaaa aaatttacgt accgaataaa gaattaaaat tatgtttcga aattatttaa
     1621 tgattgcatt gcgcaatctg aaaaaaaaca aactccatgc aactataaat attgttggtt
     1681 tggcggtggc atttatttgt ggtattttat tattcgtatt tgtacaacac gaattttcgt
     1741 acgatacttt tcataacgat aacaacaagc tttataaaac ttacaattac atacacgaca
     1801 aagatggcga atcgctcagc cctactatgg gttatccgca atcgaaaatg ttgaagaccg
     1861 aagtgcccgc agttgaatat gtatcatcgt atatgaatag tggtggttcg gtaatgtata
     1921 atgataaaga atatgaaatg ccggtttatc ttgtcgacac agatttcttt tctatgtttt
     1981 cgtttccatt agttgaatgt gatacaaaaa atccattacg cgatttgggc aatgttatta
     2041 tcacaaaaaa agcggcaaaa attattttta atcaagttga cccaataggc aaatcgctta
     2101 gcgtaaatgt gaatggtaag tggcaaacat ttgtgattgc tgccattgca gaaacaccac
     2161 cgcgaaattc aacactaaat ttcgatgttg ttttgcgtat agaaaatcgc agcgactttg
     2221 ccgaaacaaa agatgaatgg ggtaggcaac atcatcccat atatgcaaag ctgcgttcaa
     2281 attttacgca aacacaagcc gaaaaacaaa tacaaagtgc tatgcgaaaa tatgtagaag
     2341 ccgatgtaca aggattgaag aatgaaggcg caaagcccga tagcaaaggg gatgtgtata
     2401 cgataagatt gttgccaatg ctcgatgtgc atttcaatag tgaagtagga aatcaaaatg
     2461 gaattagtaa aacattctta tacatattag tattgattgg cctttttgtt tttgccattg
     2521 catgtttcaa ttttataaat ttaaatgtag cacgcgcatt tacacgtgcg cgcgaaattg
     2581 gtgttcgtaa atgtttgggt gccggtgcaa aacaaatttt tgtgcaactt gtaagtgaaa
     2641 gcgtcatcct cactttgttc gctttgatat taggaatagt tggagtggtg attgttgtta
     2701 cgcacttcac cgaaatattt gccaaccgtt tcgacttaaa tattttgtac catccgttta
     2761 caattatatt tatagttatc accagcttgc ttatatcaat tgttacaggt ggatatccag
     2821 ctttgttgat ggcaaagttt agcaccaccg aaacattgaa aggaaaagta tcacttaaaa
     2881 aaccaggcgc atttcgcaac tccctcatcg taatacaatt tgctattgca agtttactta
     2941 tttgttgcac catgattatt tattatcagt tcgattattt gcgcaacgca tcgttaggat
     3001 tcaaacaaga gaatataatc agcatacctg tatatgatgt aatgaatggt cgtgatattt
     3061 tatcgaaatt tcgcacacgt ttagcaaaca atccaacgat agaaaatgta agcggcagtt
     3121 ccatcaacat tggaattgga aacgataaaa gccaaagcaa atggcaatgt ggtttcgatt
     3181 ttaatggcca acttgtaaat acaactatgg tgaatgtggc acaagattat tttaaaacgc
     3241 ttggaataaa aataatacat ggtcgcgaat ttaccagcga atatatgtcc gatactttgg
     3301 gcagtgttat tgtaaacgaa agcatggcaa agcaattggg aaagggcaat gttgtgggca
     3361 caaaattttt gatggacagt gccgatggct tgaacatcgt ggtaggcgtg gttcccgatg
     3421 tgcatgtata ctcgatgcac gatttaactg agcctacaac ttacatcgca aatacaacat
     3481 cgcctttcag atatattttt gtaaaagtaa attccgaaaa tcccaaacag tcgctcgata
     3541 tgattgcctc tatttataaa acgatagaac ctggcaaaga atttaaaggt tcgtttatgg
     3601 atgaaaatac tcaacgatgg tatgagcaag aaaaaaatct cgccaacata tttttcgtgg
     3661 cggccgacat tgcaattatt ttatcgtgcc tcggattgtt tgcaattgca ttaataatta
     3721 ttgaacaacg caccaaagaa attggtgtcc gcaaagtttt gggtgcttct gtttttggta
     3781 tcactacttt attgtcgaaa gagtttttaa agttggtagg tatatctata ataattgctt
     3841 tgcctattgc atattttgca atgaataaat ggctgcaaga ttttccttat cgtatatcac
     3901 tacactggtg gttgtttgta ttgtcggcag gttttgcagt tattattgca ttggccaccg
     3961 tgagtttcca tagcataaag gcagcattgg ccaaaccaat aaaaagttta agaactgaat
     4021 aggggaatgc aaattggcca caaaggcgca aaggcactaa ggaatgcaat gcaattaaat
     4081 ttcgtgactt tgcaactttg tgccaaaaat tgaatttaaa aaactttgtg cctttccgcc
     4141 tttgtggcaa aaattcattg tgaataaaag cattaaaaaa ctttgtgcct ttgcgccttt
     4201 gtggcaaaaa tttattgtga ataaaagcat taaaaaactt tgtgcctttg cgcctttgtg
     4261 gcaaaaatga attgcaaaat accatacctt tatgtattaa aaaaataaaa aatgacacct
     4321 acagaacaag aagaagcaat tgggaaaaat attattactt catgcataaa aattcataga
     4381 gctttaggtc cgggactttt agaaaaggtt tatgaagtgt gtctctctta tgaattaaga
     4441 aaattaggcc atgaagtttt acggcaaatt gatattccaa tcaagtacga tgatataata
     4501 tttgacgaag gactgcgctt ggatttattg gttgataatt tagtaattat agaattgaaa
     4561 gcgattgata ttgtaaatcc tgtttgggaa gcacaagtaa tttcacactt aaaactcaca
     4621 ggaaaaaacc ttgggtatct gattaacttc aatgtaccgt tgatgaaagc tggaattaga
     4681 agatttataa attaaaggat tcagcttggc actaaggaaa tgcaaattgg ccacaaaggc
     4741 gcaaaggcac taaggaatac aatgcaattc aacattgcac cttcgtgaaa aaaatgaatt
     4801 agaaaacttt gtgcctttgt ggcaaaatat cttcgtgaca aaattgaatc gaaaaacttt
     4861 gtgcctttgc gcctttgtgg caaaaaacga attttaaaaa ataatatatg atacaattaa
     4921 aaaatatttc gcgctggtac aaaacatccg gcaataaatc gatggtgttg aatgatgtaa
     4981 gtttacacat caacaatgga gagtttgtct ccatcatggg gccatcgggg tcgggcaaaa
     5041 caacgttgtt gcaaatttta ggtatgctcg acacagccga tgatggtcag tattttttcg
     5101 aagaccaaaa tgtatttaca ttaaaagaaa aacagcgctc gctacttgca aaaaataata
     5161 ttggttttgt ttttcaagcc tatcatctga ttgatgaact caccgtttac gaaaatattg
     5221 aaactccatt aatatacaaa gatgtgaaaa gcgatgaacg caaatcgttg gtggccgaca
     5281 tgctcgataa atttaatatc gttggaaaga aagatttatt tccctctcaa ctttcgggag
     5341 gacaacagca actggttggt attgcacgtg cactaatttc taaaccaaaa gtgatacttg
     5401 ccgatgaacc tacaggaaat ttaaactcga agcaaggcga agaaattatg caattatttt
     5461 cgcaacttaa taaagaggat ggagttacaa ttattcaagt tacacactcc gaaaaaaatg
     5521 ctgaatatgg aaatagaatt gtgaggttga ttgatgggga agtttcaaat tgaggattaa
     5581 agattgaaaa ttaggaatgg gaaagaaaat tgaaaattaa ggattgaaaa ttaagaatga
     5641 tgcaagagat tgtttgtgaa gacacaaaca atggctggcg cgcaatgaac aatgactggc
     5701 agggattgct tcgcagttcg agaatactca ctgtgacaat tcgccatgac gtttgcggca
     5761 aatgaccaat gacaattctc aatgactaat aactatcctc tcaatggcca atgacaatcc
     5821 tcaatgacct tccccaattt ctggcagaga tgcttcgttc ctcagcatga caattgtgat
     5881 agcgataatg accaatgaca atcctaacaa tggctaatga ctatttccac aatgaacagt
     5941 attaaaatga cgaatgccta tcttcgataa ccaataacta aataaccaat aactcaatta
     6001 ccaataaact caggcttcct tttttcgaga aatgcattta ctccttcatt gtaatcgtgc
     6061 gatttgcctg ctgtagtttg tagttgttct tcgagggcaa gttgttgttg taaattattt
     6121 gttagcgaag cattgagagc tcgtttcgta agtccaagtg caagagtggg catggtagct
     6181 aattgtgttg caattgccat ggcttcatcg gcacatgttt cagcaacaca tactttatat
     6241 atcattccaa tttgttgcgc ttgttcggcc gatattttat cgccaagcat catgtatgca
     6301 gttgcacgtt gcatgcctat caatcggggc agggtgaatg tgccaccact atcgggtatc
     6361 aatcctattt tgctaaacgc ctgaatgaat gacgcatttg ttgcagcgat agttatatca
     6421 caagccaatg caatgttggc accggcaccg gcagccacac cattcacacc acaaactatt
     6481 ggtttttcta ttgtacgaat gcgctctatt atagggttgt agtggtcttt cactatgctt
     6541 tgtaatgcag gtccatcggc cgcaatagct tcggctaaat cttgcccggc acaaaatgct
     6601 ctgcccgcac cggtaataaa tatacaacgt atactgttat cgttagcgca ggtatcgagt
     6661 gcatgttgca tggccaatgc catttcttta ttaaaactgt tcagcacatc gggacggtta
     6721 agcgtaatta ttcctacacc atcctttatt gaaaataata ttgaagacat aaattataaa
     6781 aattaatata aggttttgtt gagcaaattc atttcggcca ctttataaaa atatttttcg
     6841 ctgcacactt tacgcataca atgtgcatgg cgtgagtggt gcatatcgct gcctatgagg
     6901 tctaccattt tttcatcaat aagttgttca gcaatttttt ttgcttctat tccataatat
     6961 cccgcaagcg agttgaaatt tatttgcaac aacactcccg aatcacgaat gcgtttatac
     7021 tcatcgaaat gtttgtaata aaaaggatac cgctcggggt gcgctaatat tggcttgtag
     7081 ccgttggttt gtattttgaa aattacatcc cgcaggttat cgggcggatt gatgtaagat
     7141 atttcgaaca gtaaataatt gtcaccaatg gtgagcaatt tttcgtggtc gagtttatgt
     7201 acaaaa
//
LOCUS       DAORQA010000076         7191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2686_length_7191_cov_15.286295, whole genome shotgun sequence.
ACCESSION   DAORQA010000076 DAORQA010000000
VERSION     DAORQA010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2686_length_7191_cov_15.286295"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..2215)
                     /locus_tag="PK736_05560"
     CDS             complement(29..2215)
                     /locus_tag="PK736_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32890.1"
                     /translation="MKQQLLRILFLIAILFAANNAQAQFVTIPDPNFVTWLNNNGYAG
                     CMNGNMMDTTCGAVVNDAIVDCSNKNISDLTGIEYFDSLKTLYCWNNQLTSLPTLPNT
                     LTYLNCAGNQLTNLPTLPNSLTDLWCSNNQLTSLPTLPNSLIDLNCYYNQLTSLPGLP
                     STLTYLRCNSNQLTSLPGLPSTLTDLWCNDNQLTSLPVLPNSLTDLNCSINQLTSLPT
                     LPSTLTYLKCYNNQLTNLPTLPSSLIVFQCYNNQLTSLPTLPSSLTDLNCHHNQLTSL
                     PALPDSLYYLYCNNNPNLYCLPKLNTIVNFNFTNTGIQCIPNYGNVTNSNPLLSSLPL
                     CDTGNTNGCVPYWNISGRVYQDTIANCSAEVNEPKYSLIKLNLYKNGLLEQTTYSNFD
                     GNYAFNTDTGLFTITIDTVSKPFVVTCPMAFSDSALISGNDTMRYNKNFAIECKPGFD
                     IGTTTIAQDSGIFRPANFANVKIFAGDISNHYGLHCSAGTSGSVQIIITGPATYISTV
                     NGALTPIVNGDTLTYTIPDFGLVDFYNDFQIQLQTDTLAQLGDQVCFDVNVTPTIGDN
                     NPANNTLTHCFTVVNSYDPNLKSVSPVNNLSINDKKLTYTIQFQNTGNAPAQHIYVMD
                     TLDAALDVSTFELLAYSHQPQVQLIGNNLRFNFPNINLPDSTNDEPNSHGYVQFSIKL
                     KDSLSIGTIIKNTSYIYFDFNPPVQTNTTSNVIISTGFNFPKHNSN"
     gene            complement(2357..2713)
                     /locus_tag="PK736_05565"
     CDS             complement(2357..2713)
                     /locus_tag="PK736_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018619271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRC32891.1"
                     /translation="MEIKPIKTNKQYETYMTWVDAQFEKGVKKNTTLGDQLEIVLMLI
                     QKYEDEKFPIPTPDAIEAIKLKMQERGIRNQDLVGEIGNKSYVSSILNKRKPLTLEIA
                     KYFHHKLGIPAAVLLA"
     gene            complement(2713..3027)
                     /locus_tag="PK736_05570"
     CDS             complement(2713..3027)
                     /locus_tag="PK736_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HRC32892.1"
                     /translation="MYDIIARKTLLAYCKKYPMAQNALLERYHELIKKEYKNFNQLKA
                     DYPNASIVSDDRVVFNILGNKFRLVVRLVFEFKTIQVKWFGTHPQYDKIDVTKIKFKQ
                     KI"
     gene            3678..4301
                     /locus_tag="PK736_05575"
     CDS             3678..4301
                     /locus_tag="PK736_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC32893.1"
                     /translation="MHPLLQMIGGGESDTLDYKKEITSAIKIAKTMVSFANHKGGRLL
                     VGVRDDKTISGIQSEEENYMLDLAANFYCKPAIAIEIIEWNYKGKIVLEVKVPNGNDK
                     PYYAKGDDEKWWVYIRVKDQSLLASKVTVDVLKKSTSNENTLVNFTSKEKALLDYLNA
                     NNRITIKEYCKLINISRWRATKTIVNLVMVGVIREHTHEAKAFYTLS"
     gene            4492..5754
                     /locus_tag="PK736_05580"
     CDS             4492..5754
                     /locus_tag="PK736_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HRC32894.1"
                     /translation="MNIKQRLIIMNFLQFFVWGSWLISIGGYLGGTLHFTGAQIGGVF
                     STLGIASLFMPAIMGIIADRWINAEKLYGMCHIIGAALLFWSSTVTDPNMFFWVMLLN
                     SMFYMPTIALNNTVSYTILEQKGFNIVKDFPPIRVWGTIGFIAAMWTVDICGWKSNNM
                     QLIFSAVSAIILGVYAMTMPACPPSKSQKDGSFLSSMGLDAFVLFKQTKMFIFFLFAM
                     FLGAALQITNAFGGSFLESFNITHPDSFGVQHPILLLSISQISETLFILTIPFFLQRF
                     GIKKVMMISILAWVLRFGLFAIGDPGSGLFLLVLSMIIYGMAFDFFNISGSLFVEKES
                     PSNIRASAQGLFMFMTNGLGAMIGGYCSGLIVDYFTVIDPATKEIVSRNWQNIWFTFA
                     AYALVLALIFPFVFKYKHDPKALENISH"
     gene            complement(6036..7001)
                     /locus_tag="PK736_05585"
     CDS             complement(6036..7001)
                     /locus_tag="PK736_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRC32895.1"
                     /translation="MKAAVYYKYGPPQVVSLAEIEKPKPADNEVLIKIFVATVNRTDS
                     GFRSAEYFVSRFFSGLFKPKFNVLGNEFAGEVEAIGKNVTLFKVGDKVFGYNDKKFGA
                     HAQYMTMAEGDTMATMPHNLSYEQAAPLTEGAHYALCDIRAAKVQSGQNVLVNGATGA
                     IGSAAVQLLKFFGANVTAVCNTANVDLVKSLGADLVIDYTKQDFTKLDAAFDFIFDAV
                     GKSSYGKCKPLLTKNGLYISTELGKNGENILLALTTPIYGGKKLLFPLPTITKDDVLF
                     LKQLAEAGKFKPVIDRTYKLEQIVEAYTYVQSGQKIGNVLLQIEH"
BASE COUNT         2119 a         1279 c         1378 g         2415 t
ORIGIN      
        1 aatgtaatag gtaatgttta cgtagatgtt aattcgaatt gtgtttcgga aaattaaaac
       61 ctgtactaat gatcacgttg ctggttgtgt tggtttgcac cggtggatta aaatcgaaat
      121 aaatgtatga tgtgtttttt ataattgtgc caatgcttaa actatctttt agtttaatgc
      181 taaactgcac atagccatga ctgttgggct cgtcatttgt actatcgggc aaattaatat
      241 ttggaaaatt gaaccgcaaa ttatttccta tcaattgcac ttgtggctga tgtgaatatg
      301 ccaacaactc aaacgtactt acatcaagcg cagcatcaag tgtatccatc acatatatat
      361 gttgtgcagg tgcattgccc gtattttgaa attgaatggt gtatgtcaac ttcttatcat
      421 taatacttaa attatttacg ggcgaaacag attttaaatt agggtcgtaa ctatttacaa
      481 ccgtgaagca atgtgtaagt gtgttgtttg caggattgtt gtcgcctatt gttggtgtta
      541 catttacatc aaaacaaact tggtcgccaa gttgtgcaag tgtgtctgtt tgcaattgaa
      601 tttgaaaatc attataaaaa tcaaccaaac caaaatcggg aattgtataa gtgagtgtat
      661 cgccatttac tattggtgta agagcaccat taacggtgct aatatacgtt gccgggccgg
      721 tgatgattat ttgcacactt ccacttgtgc ctgcggaaca atgcaggcca tagtgattgc
      781 taatgtcacc tgcgaatatt ttcacgtttg caaagtttgc tgggcggaat atgccggagt
      841 cttgtgctat tgttgttgtt cctatatcga aacctggttt gcattcgatt gcgaaatttt
      901 tgttgtaacg catagtatca tttccggata ttaatgccga atcagaaaat gccattgggc
      961 atgtaacaac aaatggtttg cttacagtat caatagtaat tgtaaataat cctgtatcag
     1021 tattgaaagc ataattgcca tcgaaattgg aataagtagt ttgttctaaa agaccattct
     1081 tatacaaatt caatttaatc aaactatact taggctcatt aacttctgcc gaacaattcg
     1141 caatagtatc ttgataaacc ctaccactaa tattccaata aggcacacag ccatttgtat
     1201 ttcctgtatc acaaagaggc aatgaagaaa gcaacggatt gctgtttgtt acattaccat
     1261 aatttggaat gcattgaatg ccggtatttg tgaaattaaa atttacaatt gtatttagct
     1321 taggcaggca atacaaattg gggttgttat tgcaatataa ataatacaac gagtcgggca
     1381 aagcaggcag gcttgtgagt tggttatgat ggcagtttaa atcagtcaaa gaacttggca
     1441 aggttggcag acttgtgagt tggttgttat agcattgaaa aacaatcaaa gaacttggca
     1501 aggttggcag atttgtgagt tggttgttat agcattttaa atatgtcaaa gtacttggca
     1561 aggttggcag acttgtgagt tggttgatag agcaatttaa atcagtcaaa gaatttggca
     1621 agacaggcag gcttgtgagt tggttgtcat tgcaccataa gtcagtcaaa gtacttggta
     1681 agccaggcag gcttgtgagt tggttgctat tgcaccttaa gtaagtcaaa gtacttggta
     1741 agccaggcag gcttgtgagt tggttgtagt agcaatttaa atcaatcaaa gaatttggca
     1801 aggttggcag acttgtgagt tggttgttac tacaccataa atcagtcaaa gaatttggca
     1861 aggtaggcag attagtgagt tggttgccag cgcaatttaa ataagtcaaa gtgtttggca
     1921 aggttggcag acttgtgagt tggttgttcc agcaatataa ggttttcaag ctatcaaaat
     1981 attcaatccc agttaaatca cttatatttt tattagaaca atccacaatg gcatcattca
     2041 caaccgcccc acaagtagta tccatcatat tgccattcat gcagcctgcg tagccgttgt
     2101 tatttagcca tgttacaaaa tttgggtcgg ggatggttac aaattgcgct tgcgcattat
     2161 tagctgcaaa aagtattgca atgagaaata aaatgcggag tagttgttgt ttcatttttt
     2221 aataatgtaa agggcacagt tattttaaat cgatgtaaat ataaaatatt tagggcaaga
     2281 atcgtgctgt tgtttttatt atttttaggc actaagaggc acaaagaaat tcgaatgcaa
     2341 aaaatattat tactacctat gctaaaagta cggccgctgg tatgcccaat ttgtgatgga
     2401 aatattttgc aatttcaagt gtcaatggct tccgtttatt gaggatggat gatacgtaac
     2461 ttttgtttcc aatttcacca accaggtctt ggttgcgaat accacgttct tgcattttca
     2521 acttgattgc ttcaattgca tcgggtgttg gaatcggaaa cttttcgtct tcgtactttt
     2581 gtatcagcat caatactatt tctaattggt cgcccaaagt tgtatttttt ttaactcctt
     2641 tttcaaattg tgcgtcaacc caagtcatat aggtttcgta ttgcttgttt gtttttattg
     2701 gtttaatttc cattatattt tttgtttaaa ttttattttt gtaacatcaa ttttatcata
     2761 ctgtggatgt gtaccaaacc attttacctg tatcgtttta aactcgaaaa ccaatcgaac
     2821 tactaatcgg aatttatttc ctaaaatatt aaacaccact ctgtcatcgc ttactatgct
     2881 tgcattaggg tagtcagctt taagttgatt aaaatttttg tattctttct ttatcaactc
     2941 atggtacctt tccaataatg cattttgagc cattggatac tttttgcaat aggccaataa
     3001 agtttttcgg gcaatgatat cgtacattga tgcaaaataa agaagttttc tattggcaaa
     3061 caagcgcttt gcaataaaat gttcacgttc cacatttttt attgctgcct atgcctttct
     3121 tttaatttta acctcaacat tcttcctccg attattgttg aaagaagcaa tcctcatggc
     3181 tcaacttcct tccgatgccg ggattgcttc agtagttcct ccttcgtaat gacgtgagga
     3241 gattgacgta tatgttttag ctatcgagtc tctcataaca aaaaatcaaa agagaatgcg
     3301 gactctaaag cggtttctat gccgcgcctg ccccgaccgc agtgtcgggg aatgcacgaa
     3361 tgaattccga ataaaatgca aatggctttt agatgctgag attgcttcag tcgttcctcc
     3421 tacgcaatga cgtgaggaaa gttgttgtta gctttcgagt ctcttatgcc aataaaaaca
     3481 aaagagaatg agaactctaa atcgttttat ttacagagta tgcacgaatg aattccgaat
     3541 aaaatacaaa tgctgtacaa tacaactttg tgtctttgca cctttgtggt tatttttttt
     3601 cagggcaatt cttttatcac taaatttttt gtttagcctt tattttattt atttgccact
     3661 ataaactaac aagcaatatg catccgttgt tgcaaatgat aggtggaggc gaaagcgaca
     3721 cgctcgatta taaaaaggaa ataacttcgg caataaaaat tgcaaagacc atggtatcgt
     3781 ttgccaatca caaaggcgga cgattgcttg tgggtgtgcg cgatgataaa accataagcg
     3841 gcatacaaag cgaagaagaa aactatatgc ttgaccttgc agcaaatttt tattgcaaac
     3901 ctgccatcgc catcgaaatt attgaatgga attacaaagg taaaattgtg ctcgaagtaa
     3961 aagtgcctaa cggtaacgat aaaccctact atgcaaaagg cgatgacgaa aaatggtggg
     4021 tgtatattcg tgtaaaagac caatcgctat tggcaagcaa agtaacagtt gatgttttga
     4081 aaaaaagcac tagcaatgaa aacacacttg ttaattttac atcaaaagag aaagccttgt
     4141 tagattattt aaatgccaac aaccgcatca ccattaaaga atattgtaag ctcataaata
     4201 tttcgcgttg gcgtgctacc aaaacaattg taaatctagt aatggttgga gtaattcgtg
     4261 aacacacgca cgaggcaaag gcattttata ctttgagtta aaagctgttt acgcaacata
     4321 agccttttcg gtgtcctcat tatcgtttgt ttttttaaaa ggtacgagag actctgaaac
     4381 ggaattaaaa aaatgttcgg gctctgaaat ttgagcaggc ttcaaatttt tgtattcaca
     4441 aatattttcc atattgcagc ttctaaattt tacatcttaa atcttattta tatgaacata
     4501 aagcaaaggt taataataat gaacttcctt caattttttg tttggggttc gtggttgatt
     4561 tctattggcg gatatcttgg cggtacgctt cactttaccg gtgcacaaat tggcggagta
     4621 ttttctacgt tgggtatagc atcgcttttt atgcctgcca ttatgggtat aattgccgac
     4681 agatggatta atgccgaaaa gctttacggt atgtgtcata ttattggtgc agccttatta
     4741 ttttggagct caacagtcac cgatccaaat atgttttttt gggtaatgtt gctcaactct
     4801 atgttttata tgccaaccat tgcactcaac aataccgtct cgtataccat actcgaacaa
     4861 aaaggattta atattgtaaa ggacttccct cctattcgcg tatggggcac tataggcttt
     4921 attgcagcca tgtggacagt tgatatatgc ggatggaaat cgaataacat gcagcttatt
     4981 ttcagtgcag tgtcggccat tattttgggc gtatatgcta tgaccatgcc ggcatgtcca
     5041 ccatcgaaat cgcaaaaaga tggttcgttt ctttcgtcca tggggctcga tgcttttgtt
     5101 ttatttaagc aaaccaaaat gtttatcttc tttttattcg ccatgtttct tggtgcggct
     5161 ttacaaatta caaatgcatt tggcggttcg tttctcgaaa gtttcaacat cactcatccc
     5221 gattcgtttg gtgtgcaaca tcccatttta ctgttatcca tttcacaaat ttccgaaaca
     5281 cttttcatac tcaccatacc attttttctg caacggttcg gcataaaaaa agtaatgatg
     5341 ataagtatac ttgcatgggt tttacgtttc ggattattcg ccattggtga cccgggcagc
     5401 ggattgtttt tgctcgttct ttcaatgatt atttatggta tggcattcga ctttttcaat
     5461 atatcgggtt cgttgtttgt agaaaaagaa tcgccttcaa atattcgcgc gagtgcacag
     5521 ggattattta tgtttatgac aaacgggctg ggcgctatga ttggcgggta ttgcagcggt
     5581 cttattgttg actacttcac cgtaatagat cctgctacaa aagaaatagt atcgcgcaac
     5641 tggcaaaaca tctggttcac ttttgctgcc tatgcacttg tgttggcact tatatttcct
     5701 ttcgtattta agtacaagca tgatccaaaa gcgttggaaa atatttcaca ttaattttat
     5761 ttattttgca aaaatgaatc ttaccgttca aactgtttct gtatggtgtt ttattttttg
     5821 agtctactcc gataaattat ttttgccaca aaaacactaa ggcacaaaat tccactaaga
     5881 tgtaacaaat catttttaat gttttgtgga atttggtgtt ttggtgaatt agagctcttt
     5941 aatattttag acatttcgga gcggactcat tcttcacatc ttaaatataa tacaattact
     6001 caaacgttta tacctacagt tattacatca gcaaatcaat gttctatttg caataaaaca
     6061 ttgcctatct tctgccctga ttggacataa gtatatgctt ccactatttg ttcgagtttg
     6121 taggttcggt ctattacagg tttaaatttg ccggcttcgg caagctgctt caaaaaaagc
     6181 acatcgtctt ttgtaattgt tggcaaggga aataataatt tcttaccacc gtatataggt
     6241 gtggttaatg ccagcaaaat attctctccg ttctttccca actcagttga aatgtataat
     6301 ccatttttag tgagcaaagg tttgcatttt ccataagaac ttttacccac agcatcaaaa
     6361 atgaaatcga aagcggcatc caattttgta aaatcttgtt ttgtataatc aataaccaga
     6421 tcggctccta acgattttac caaatcaaca ttggctgtat tacacactgc cgtaacgttg
     6481 gcaccaaaaa attttaacag ctgcactgct gccgatccta ttgcacctgt agcaccattt
     6541 accaatacat tttgtccact ttgcactttt gcagcacgta tatcgcacag ggcataatgt
     6601 gcaccttcgg tgagtggagc tgcttgttcg tacgataaat tatggggcat ggtggccata
     6661 gtatcaccct cggccatggt catatactgc gcatgcgcac caaatttttt atcgttgtaa
     6721 ccgaatactt tatcgcccac tttaaacaac gtaacatttt ttccaatggc ctctacctca
     6781 ccggcaaatt cgttgcccag cacattgaat tttggtttaa acaacccgct aaaaaaacgc
     6841 gatacaaaat attcggcact tcgaaaacca ctgtcggtgc ggtttacggt agccacaaaa
     6901 attttaataa gcacttcatt atcggcaggt ttaggctttt caatttcggc aaggctcacc
     6961 acttgtggcg ggccatattt ataatatacg gcagctttca ttttttttat tgattagttt
     7021 tttttacaaa tagaattaag aatgattaaa aaacggaagc acaaaataga agaaaaatcg
     7081 tgtaataaac tattacaaat gtggttacaa gttctactat tgacgataat ggttagcttt
     7141 tatatttttt tcggctaatg ccactgtttc atcaattctt ttttggcggg t
//
LOCUS       DAORQA010000077         7110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2717_length_7110_cov_6.899929, whole genome shotgun sequence.
ACCESSION   DAORQA010000077 DAORQA010000000
VERSION     DAORQA010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2717_length_7110_cov_6.899929"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="PK736_05590"
     CDS             complement(<1..1380)
                     /locus_tag="PK736_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC32896.1"
                     /translation="MIKNYLLLTFRNMSRNKVFAFINIFGLSIGLAACILIFLYTKDE
                     MSFDRFHQNTSRLYQLTCTVKSHDGKLETYGIAGNIHAEAFKAEIPEIKEFVRLRDDD
                     LVIKIGDQSYEEKLWWADKNFFSVFSFPLIEGDKNSVLKDMKSMVISEEYAKKYFGND
                     NAIGKIIEVEFDDQFVPFTVTGIAKKTPQNSSIKFDMIASFDYLQSMNPKPAWFMLSY
                     PSFLVLNENADLKAIANSMQKVYNSKSGYQKMEKDGFDSKFIWGLQPFVDMHLNKDIK
                     YSMSVSDPVYSYILSCIALFILIIACINFVNLMVAQSLKRSKEIGVRKVIGGSRKQLF
                     VQFIGEAFTMCFFAFGLAFLIAELSLPYFNTLAEKELSIGYLFDAKLIAGFVLLFLIT
                     VLASGFYPALVLSGFDPVRTLYNRTTFSSKNYLVKGLVVLQFSLATFLIIGTLFIYNQ
                     LNFLKKVDLG"
     gene            complement(1393..3783)
                     /locus_tag="PK736_05595"
     CDS             complement(1393..3783)
                     /locus_tag="PK736_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC32897.1"
                     /translation="MMLYHNIKLTLRNFKHNASFSLLNLIGLSIGITVVVLLMAYIQN
                     ESTYDSYHKNAKRIYRVTVLENDDGKINVLANAPNAVGPTAQEFIPELESQVRILKNN
                     FGDPTFVSTAKDNFIEKNYFWCDASYFKIFDVNILSGNPLDLNKPNKVFLSKSKARQY
                     YGNENCVGKTILVNGSKQIEVAGVYDDLPQNTSMRMDFIASYASTSFSRNPNTWDNAS
                     FETYVLLKENVAPLIVEKKLAQMISTKIPKDEQWFQLQLQALTDVHLHSANAMWNYSS
                     DLNDYATLKHLALLALLIIIIACVNYMNLSTARLQKRMKEIGISKTLGASFKTLFIRF
                     YTETAILIFVAIVFSFLLSIILLPVFANVISKPLSLQLLFHPYVLLGYVILWLGITAL
                     SGLYPSYMLASANVTATLSQRNKLNANHPLLRKILVTSQYAASVILIIAMIVIQQQLN
                     FIRNKNLGFNPENIVAISTVASKTDEPVNALINELKNLPEVKTICRSQSFPGIQSSGR
                     SIYKNDADENGFPLTTNRASAEILKTLNLKLLAGNVLPINKSKDDTLVEVIVNKTAID
                     YLGLKPEDAIGKKISCQLGNNSYITGVVDNFNFESLHQPVSGYAFHNASREPFSFLLV
                     QYNTKNMQQLISKIETIFKKTNPQSAFDYTFLDKQMQSLYQSEQRSALVTMSFSILAI
                     LIASLGLFGLAAYTAEQRTKEIGVRKLLGATVTSIATLLSRDFVKLVFVAIIISIPFA
                     YWLMNNWLQGFAYRININVWMFATAGLFAMVIAIVTVSFQAIKSALANPIKSLRTE"
     gene            complement(3839..6214)
                     /locus_tag="PK736_05600"
     CDS             complement(3839..6214)
                     /locus_tag="PK736_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC32898.1"
                     /translation="MNSIINIVGLAIGLAAYLFLLQYITIEKSVNQFHTDISSTYRIM
                     NEGPDGKTWPQVEPGWAPLIKQRVPEVQDYCRFEEGVCQGIVTTDGNATSYREKAIGY
                     AEGNFFDFFTFPLVAGNAKSFNASQVVFISKSTSQKYFGTQDALGKVLTLNNQFGVMP
                     FTIKGVYEDMKDNSDIRYDMLFSLETIKNPANLNENSWAELGNLNSQYIYTYLKLSIT
                     QNIVTTQNKLTALRTELKPDKDGVKFKLQPLANIHLGTSLNDTFQTTGNLKYIYLLTG
                     ISMLILVIAWFNYINLATANAIKRAGEIGVRKVIGASRFNIIYQFVIESLLLNGLGFL
                     LAIVLVLLFQPVYNNVINRSLSIQSITSGTAWISGLSILIAGAFISGFYPAFTLSKYN
                     PVDTIKGKIKSTAKGLLLRKSLVVSQFTISTVLIIATFIIYMQLHYMQSEALGVNTQQ
                     LIVISGPDAGKDSTFKNRSIGFEAQLAQQNFIKAFCFSGSVPSGWYNFTTGGFTQPGS
                     KKGDELKSYSFAIIGDKFLNTYEIKLKAGRNFTPQECNVSWNANSKVMVNETALKQLG
                     FNNAEQALSTKIQWDERKLEIIGIVSDYHHTSLKNAIDPIIFYPQNNSAHITVRLTPD
                     NFPKKIAQIESLFKAAFPGNPFEYSFVDDNYNKSYISETQYGNIFTAASLCAIFIACL
                     GLFGLATFTVESRVKEIGIRKVLGASVNNIVTLLSKDFMILVLVALIIATPIGYYLMN
                     KWLKDFAYRIEIPAWIFIVAGMLALLVAAVTISYQSIRAAIANPAVSIRTD"
     gene            complement(6278..6955)
                     /locus_tag="PK736_05605"
     CDS             complement(6278..6955)
                     /locus_tag="PK736_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019038297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC32899.1"
                     /translation="MIKITNLEKYYRTDEVETVALNKISIEIKQGEFVAVMGPSGCGK
                     STLLNVLGLLDDADNGSYLFNGTEVIKYNERQRAQIRKQNIGFVFQSFNLIDELSVYE
                     NVELPLIYLDVKPSERKTRVEEVLDKMQIMHRKKHFPQQLSGGQQQRVAVARAVVNNP
                     KLILADEPTGNLDSSNGNEVMQLLTDLNEKGTTIIMVTHSEHDSKYSHRIIRMLDGAT
                     VTENILM"
BASE COUNT         2229 a         1319 c         1266 g         2296 t
ORIGIN      
        1 acctaagtct acttttttca aaaagttaag ttgattataa ataaataatg ttccaataat
       61 taaaaaagta gcgagcgaaa actgcaaaac aacaagaccc ttcactaaat aatttttgct
      121 gctgaatgtg gtgcgattgt aaagtgtgcg aacagggtcg aagcctgaaa gtaccaatgc
      181 cggataaaat cctgaagcca atacagtaat taaaaaaagt aaaacaaagc cggcaattaa
      241 ttttgcatcg aataaataac caatacttaa ttctttttcg gcaagggtat tgaaataggg
      301 taacgataat tcggcaatta aaaatgcaag gccgaatgca aaaaaacaca tggtaaacgc
      361 ttcaccgata aattgcacaa aaagttgttt gcggctaccg cctatcactt tgcgcacacc
      421 aatttcttta cttcgtttta atgattgtgc caccataagg tttacaaaat tgatacaggc
      481 aattattaaa ataaataatg ctatacaact taatatatac gagtacacag ggtcgcttac
      541 actcatagag tatttaatat ctttgttgag atgcatatca acaaatggct gcaatcccca
      601 aataaattta ctatcgaagc cgtccttttc catcttctga taacctgatt tactgttgta
      661 aactttttgc atactgttcg caatggcctt taaatcagcg ttttcattca ataccaaaaa
      721 cgaagggtac gataacataa accatgcagg cttaggattc atagattgca aataatcgaa
      781 cgatgcaatc atatcgaatt tgatacttga gttttgcggg gtctttttag caatacctgt
      841 tacagtaaat ggaacaaatt ggtcatcaaa ttctacttcg ataatttttc caattgcatt
      901 gtcatttcca aaatactttt ttgcatactc ttcactaatc accatggatt tcatatcctt
      961 taaaaccgaa tttttgtctc cttcaatcag cggaaaagaa aaaacagaga agaaattttt
     1021 atcggcccac cacaattttt cttcgtaaga ttgatcgcca atttttatta ccaaatcatc
     1081 atcgcgaagg cgcacaaatt cttttatctc aggtatctca gctttgaaag cttcggcatg
     1141 aatgtttcct gcaatgccat aagtttctaa tttgccatca tgactcttta ctgtacaggt
     1201 aagttggtac agcctactgg tgttttggtg aaacctatcg aaactcattt cgtcttttgt
     1261 ataaagaaaa attaatatgc atgcggcaag gccaatactt aatccaaaaa tattgatgaa
     1321 tgcaaatact ttgttgcgcg acatattgcg aaacgttaga agcaaataat tttttatcat
     1381 ggttttaaat ttttattcgg tacgtaaact ttttattgga ttggcaagtg cagattttat
     1441 ggcttgaaaa cttactgtaa caattgcaat taccattgca aacaaccctg ccgttgcaaa
     1501 catccacaca ttgatgttga tgcgataagc gaaaccctgc aaccaattat tcataagcca
     1561 ataagcaaaa gggatagaaa tgatgatggc cacaaaaaca agtttcacaa aatcgcgcga
     1621 gagtaaagta gctatgcttg taaccgtagc accaagcaat ttgcgcacac caatttcttt
     1681 tgtacgctgt tcggcagtat aagctgcaag accaaaaagg ccaaggcttg caataagaat
     1741 agcgagtata gaaaatgaca tggtaacaag tgcactgcgc tgttcactct gatataaact
     1801 ttgcatttgc ttatcaagaa atgtataatc aaatgccgac tgaggattag tttttttaaa
     1861 tatggtttca attttactta tcagttgctg catgttctta gtattgtatt gcactaacaa
     1921 aaatgaaaat ggttcacggc tggcattgtg aaatgcatat cccgaaacag gttgatgcag
     1981 cgattcgaaa ttgaaattgt caaccacacc ggtgatataa ctgttattgc caagctgaca
     2041 acttattttt tttccaatgg catcttcagg tttcaatccg agataatcga ttgcagtttt
     2101 atttacaatc acctctacaa gtgtatcgtc tttgctttta ttaatgggca aaacattgcc
     2161 ggccaaaagt ttaagattta atgttttcaa tatttcggcc gatgcgcgat tggtggttag
     2221 tggaaaacca ttttcgtcag catcgttttt ataaatcgaa cgaccgctac tttgtattcc
     2281 gggaaaagat tgtgagcggc aaatggtttt tacttcaggt aaatttttca attcatttat
     2341 taatgcatta acaggctcat ccgtttttga tgcaacggta cttattgcca caatattttc
     2401 aggattgaat cctaagtttt tatttctgat aaaattcaat tgttgttgaa ttactatcat
     2461 ggcaataatt aaaataaccg atgcagcgta ttgcgatgta actaatattt tgcgaagcaa
     2521 cggatggttg gcatttaact tattgcgttg agaaagtgtt gccgtaacat tggccgatgc
     2581 aagcatatat gaggggtaca agccagaaag cgctgttatg ccaagccaca gaataacata
     2641 gccaagcagt acatatggat gaaataataa ttgcagcgat aatggtttgc tgattacgtt
     2701 agcaaacacc ggcaacaaaa taatgcttag caaaaaagaa aaaacaatgg caacaaaaat
     2761 caaaattgcg gtttcggtat agaagcgaat gaataatgtt ttgaatgatg caccaagtgt
     2821 tttcgaaatg ccaatttctt tcattcgttt ttgcaaacgc gcagtagaaa gattcatata
     2881 atttacacag gcaataatta taatgagcaa tgcaagtagc gcaaggtgtt ttaatgttgc
     2941 ataatcgttg aggtcgctgc tgtaattcca catggcattg gccgagtgaa gatgcacatc
     3001 agtcagtgct tgcagttgca attgaaacca ctgttcgtcc tttggaattt ttgtggaaat
     3061 catttgcgct aatttttttt ccactattag tggagccaca ttttctttta ataacacata
     3121 agtttcgaaa ctggcattgt cccatgtatt gggattgcgc gaaaaacttg ttgatgcata
     3181 cgaagcaata aaatccatac gcatgcttgt gttctgcggt aagtcatcgt acacaccggc
     3241 aacttctatt tgcttgctac catttactaa aatggttttg ccaacacaat tttcatttcc
     3301 ataatattgc ctggctttgg acttcgataa aaaaacctta ttcggcttat ttaaatcaag
     3361 cgggtttccc gataaaatat ttacatcgaa aattttgaaa tacgatgcat cgcaccaaaa
     3421 ataattcttc tcaataaaat tatcttttgc agtgcttaca aatgttgggt cgccaaaatt
     3481 atttttcaaa attcgcacct gcgattctag ttcaggaata aattcctgtg ctgtgggccc
     3541 tacagcattg ggggcattgg cgagcacatt tattttacca tcgtcatttt ctaaaacagt
     3601 tacacgataa attctttttg cgtttttatg ataactatca tacgtagatt cgttttggat
     3661 atacgccatc aacagcacca ctaccgtaat tccaattgat aagccaatca aatttagtaa
     3721 actaaatgaa gcattgtgct taaaatttcg cagtgtgagt tttatgttgt ggtatagcat
     3781 cattgttagt attttttgtt tttatcatta ataatcaaaa catatttcac tttgtatatt
     3841 aatcggtacg aatgcttacg gcagggttag caatagccgc tctaatactc tgatagctta
     3901 tagttactgc cgccacaagt aatgctaaca tgccggcaac aataaaaatc catgcaggaa
     3961 tttcgatgcg ataagcaaaa tctttgagcc acttattcat taaataatat ccaattggtg
     4021 tggcaataat taatgctact aaaaccaaaa tcataaaatc tttcgaaagc aaagtcacaa
     4081 tgttgtttac acttgcacct aaaacttttc taattccaat ttctttaaca cgcgattcaa
     4141 cggtaaatgt tgccaggcca aaaagtccta agcatgcaat aaaaatggca cacaacgatg
     4201 ccgctgtaaa tatgttgcca tactgtgttt cgcttatata actcttgttg tagttatcat
     4261 caacaaatga atactcgaat ggattacccg ggaatgctgc tttaaatagt gattctattt
     4321 gagcaatttt tttaggaaaa ttatcgggcg ttaatctgac tgtaatgtgt gccgaattat
     4381 tttgaggata aaaaataatt gggtcaattg cgttttttaa actggtatga tggtagtcgc
     4441 tcactatgcc aataatttcc aacttgcgtt cgtcccattg tatttttgtt gatagcgctt
     4501 gttctgcgtt gttaaaacct aattgcttca gtgctgtttc atttaccatc actttactat
     4561 tggcattcca tgatacattg cactcctgcg gagtaaaatt tcggcccgct ttaagtttta
     4621 tttcgtacgt gttcaaaaac ttgtcgccaa taattgcaaa ggaatatgat ttaagttcgt
     4681 cacctttttt cgaaccgggt tgtgtaaagc ctccagttgt aaagttgtac catccacttg
     4741 gaacactacc cgaaaaacaa aaagctttta taaaattttg ctgcgcaagc tgagcttcaa
     4801 atccaatgga acggttttta aaagtagaat ctttgccggc atcgggacca ctaattacta
     4861 tcaattgttg tgtattaact ccaagtgctt ccgattgcat gtaatgcagc tgcatatata
     4921 tgataaacgt agcgatgatt aaaacggtag agatagtaaa ttgcgaaacc accaacgact
     4981 tacgaagcaa caatccttta gcagttgatt ttattttccc ttttatagta tcaacaggat
     5041 tgtatttgga caatgtaaaa gccggataaa accctgatat aaatgcacct gcaattaaaa
     5101 ttgataaacc tgaaatccat gcagtgcccg atgtaatact ttgtatgctt aatgagcggt
     5161 ttataacatt attataaaca ggttgaaaaa gcaatactaa aacaatggca agcaaaaatc
     5221 ctaaaccatt taacaacaac gattcaatta caaattgata aataatattg aaacgcgaag
     5281 cgccaataac ttttctaaca cctatttcac ctgcacgctt tattgcattc gctgttgcaa
     5341 gattaatata gttgaaccat gctatcacta aaatgagcat cgaaattccg gtgagcaagt
     5401 agatgtattt taaattgcct gtagtttgaa aagtatcatt gagcgatgtt cctaagtgaa
     5461 tattagcaag cggttggagt ttaaatttca ctccatcttt atcgggttta agttcggtgc
     5521 gcagtgcagt gagtttattt tgggttgtaa caatattttg ggtaatgcta agcttgagat
     5581 aggtgtaaat gtattgggaa tttaagttgc ccaattcggc ccaggaattt tcgttcaagt
     5641 tggcggggtt cttaattgtt tcaagagaaa aaagcatatc atagcggata tccgaattat
     5701 ctttcatatc ctcataaaca ccttttatgg taaaaggcat cacgccaaat tgattgttga
     5761 gcgttagcac ttttcccaaa gcatcctgtg taccaaaata tttttgactt gtggatttcg
     5821 aaatgaaaac aacttgtgaa gcattaaaag attttgcatt cccggcaact aaagggaagg
     5881 taaaaaaatc gaaaaaattt ccttcggcat aacctattgc cttttcacga tatgatgttg
     5941 cattgccatc tgttgtaaca attccctgac aaacgccttc ttcaaaccgg cagtagtcct
     6001 gcacttcagg cactcgttgt tttattaatg gtgcccagcc cggttccacc tgaggccatg
     6061 ttttgccatc aggaccttcg ttcattattc tataggttga tgatatatca gtatgaaatt
     6121 ggttcacact tttttcgata gtgatgtatt gtaataaaaa taaatatgct gctaaaccaa
     6181 ttgccaaccc aactatgttg atgatagaat tcactttatt tctacagaca tttctaaagg
     6241 caattttaat atggtttcta aacattgatt ttatttttta catcaaaatg ttttcggtaa
     6301 cggtagcacc atcgagcata cgaataatac gatggctgta tttgctatcg tgctcgctgt
     6361 gtgttaccat aatgattgtt gttccttttt cgtttaggtc ggtaagcaat tgcattactt
     6421 cgttaccatt gctgctatcg agattaccgg taggctcatc ggccaatatt aatttaggat
     6481 tatttacaac ggcacgtgca acggccacac gctgttgttg acctccgcta agctgttgtg
     6541 gaaaatgctt tttgcgatgc attatctgca ttttatcgag cacctcttct acacgcgttt
     6601 tgcgctcgct gggttttaca tcgagataaa ttaatggaag ctcaacattt tcatacacgc
     6661 tcaattcatc aatcaaattg aaactctgaa aaacaaaacc aatattctgt ttacggattt
     6721 gagcacgttg acgctcgttg tattttatta cttcggtacc gttaaataag tagctgccat
     6781 tatcggcatc gtcaagcaaa ccaagcacat tgagcaaggt agatttgccg caacccgaag
     6841 ggcccattac tgcaacaaat tcgccttgtt ttatttcgat agaaattttg tttagcgcta
     6901 cagtttccac ttcatctgtg cgataatact tttcgagatt ggtgattttt atcataaatt
     6961 attttttata ggaatcgatt ttttttaaat acaatctaga cacaaaggct caaagttttt
     7021 ttatgttact cagagactat gttcttttta atactttttt gccacgcctg cctcgaccgc
     7081 agtgtcgggg aatgcacgaa tgaataccga
//
LOCUS       DAORQA010000078         7100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2722_length_7100_cov_9.125053, whole genome shotgun sequence.
ACCESSION   DAORQA010000078 DAORQA010000000
VERSION     DAORQA010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2722_length_7100_cov_9.125053"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1425
                     /locus_tag="PK736_05610"
     CDS             <1..>1425
                     /locus_tag="PK736_05610"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRC32900.1"
                     /translation="FILFFKFFMSGSFDNGGYESMTRVLEVLAGFSLGAESIALFARV
                     GGGIYTKAADVGADLVGKVEAGIPEDDPRNPATIADNVGDNVGDVAGMGADLFGSYVA
                     TVLASMVLGNYVIRDFVEAYGSIANIPFSGMGPIFLPLSIAAIGILASIVGTFFVNIS
                     DKQEANTNTVQSALNRGNWISIILSLVGAFVLIKLCLPETMTMRIFGNGEFSNMTTSS
                     MNVFYAVMIGMFVGGAISYITEFYTAIGKGPVNGIVQKSGTGAATNIIAGLGTGMLST
                     AMPVILFAGAIWGAYALAGFYGVGIAASAMMANTAMQLAIDAFGPIADNAGGIAEMSH
                     MPEDVREKTDILDSVGNTTAAIGKGFAIASAALTALALFAAYVTFTGIQGINIFDAKV
                     LAALFIGGMIPVVFSALAMNSVGKAAMDMVNEVRRQFREIPGIMEGTGMPEYSKCVAI
                     STQAALREMLLPGAITIITPIIVG"
     assembly_gap    1426..1464
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1465..2465
                     /locus_tag="PK736_05615"
     CDS             <1465..2465
                     /locus_tag="PK736_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRC32901.1"
                     /translation="AYMAGVTVSGVVWAIFQNNAGGAWDNAKKSFEKGVMINGKLEKK
                     GSDAHKAAVVGDTVGDPFKDTSGPSMNILIKLSCLVGLTLAPLLGHNESNAGACCEED
                     AKCEMVCDMEKCKSMTPEQCAAYCDSMGCSADEKNKCMEMCKGNASMPATAMPSLDVA
                     TMKGMYIIDQSHSFVDFKLRHLLTDAKGSLAIDTAYFDLTDDLTTSKVFIQLDVKSID
                     TKNKERNEHLLKDDFFDVAKYPKITFNGNSIRKSEKANYAYVITGDLKMKDVTKPVDL
                     YFNYIGNIENEYAKELAHSFAGMMEIKRTDFKVGTPSTMVGDKVAIDFSVETYMKK"
     gene            2712..2903
                     /locus_tag="PK736_05620"
     CDS             2712..2903
                     /locus_tag="PK736_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32902.1"
                     /translation="MLFLNTTTNKMSTIAGDEILKRYSVFFEMANATNKAAIAIKQQT
                     VATANIIPLNLSFSIPDII"
     gene            complement(2926..4317)
                     /locus_tag="PK736_05625"
     CDS             complement(2926..4317)
                     /locus_tag="PK736_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRC32903.1"
                     /translation="MTRIIQLLKDAFNSSAHDFTTISIRRAVVLLAIPMILEMCLESV
                     FAVVDLFFVNKLGPGAVSIVGLTESVTAIVYSVAIGLSSAATAIVARRIGEKNNRDAS
                     KAAAQATLMAVVVSLVMGVIGFVFAKNILQFMGAAPSAIEAGLNYTRIQLGTSIVVVL
                     LFMLNGIFRGAGDAALAMRSLWVANICNIILCPVLINGYGPFPQMGITGAAVATCIGR
                     GIGVLYQVYFLIKAKHIIHFYTEDFVPEWLSQKALADIATPAIFQFLISSASWIFLAA
                     IVAQSGVIASAGYQTAIRLVIFFILPAWGLSNAAVTLVGQNLGAQQPQRAEQAVMKTA
                     KYNAIFMAMVTLLFYTCGEWIIRFFTPANQPEQIAIAVQALKYISSAYIFYGIGMVLM
                     LSFNGAGDTRTPTIISFVGFWLLQIPLAYLLSHHFQLNYLGVFIAIPFSETVMAIISY
                     FVFKQGKWKTVKV"
     gene            complement(4427..6946)
                     /locus_tag="PK736_05630"
     CDS             complement(4427..6946)
                     /locus_tag="PK736_05630"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32904.1"
                     /translation="MKKISFIVLSAIAVASAILVFTKKHNTPTPKISALPAEDFFMMR
                     NYPDFTMDVKGYEQAMQITKQQSLQSKLANTVGFNNAWTMEGPGNIGGRINCVAMHPT
                     NNMIMYVGNATGGIHKTTDGGVTWNPIFDDQSALSVSCIEFEPGNPNTMWVGTGDAQI
                     SGYPFIGDGIYKSTDGGNTWTNMGLANTRIITKIKIHTTNPNIIYAATMGVPFIKDNN
                     RGVYKSIDGGVTWQQSLFISDSAGVIDMLMDPQNPDVLYAAGWNRLRSNQRSLISGPD
                     ARIYKTTDGGTTWNMLTNGLPIIDASRIGLTMSGTNANVIYASYVDVNFDLLGIYKTT
                     NGGTSWTQKGGNNLLGVQGGFGWYFGRIEINPTNDNELYFCAIGLHKSTDGGDNFYDV
                     DPAYDMHADKHDIRYLSNSVQLCATDGGLYKTTNGGSTWNDIENIPNTQFYRCAVNPH
                     AQGVYAGGAQDNGTEYGNAASFNNWIRVFGADGFQPLYHPTDPNIMWAEYQNGGINVS
                     TDGGNSFNDATNGIDFNDRRHWDMQYIMSKADPNRMYTGTYQVYRNDDGENANWYSIS
                     SDITDGVIYGDRFHTISCVEESPANDNNLYAGTTDGNVWASTNQGTTWQNVTGVLPDR
                     YVSSVKASVLNANTVFVTHTGYKYNDFIPHIHKSINNGNTWVDISGNLPPAAINDVYV
                     TAAGDDVLFVATDAGVYATVDGGVTWNRLGNNMPIIPVYDLDFDYSSNRLCAATFARS
                     MMTYDVDSIVSLGTPAVSELPSLSLYPNPAINYLQIKLPFTNDVTITIYDVKGNKCMQ
                     QNFEKQIPKKINVEMLTRGIYFVEVQSGNKRFYGKFLKGEL"
BASE COUNT         2103 a         1397 c         1390 g         2171 t
ORIGIN      
        1 tatttatact gtttttcaaa tttttcatgt cgggttcttt cgataatgga ggttatgaat
       61 ctatgacccg cgtgcttgag gtgctagccg gattttcgct tggtgccgaa tctattgcat
      121 tgtttgcccg tgtaggtggt ggtatttata ctaaagctgc cgatgtgggt gccgacctag
      181 taggtaaagt agaagccggc attcctgaag acgatcctcg caacccggca accattgccg
      241 ataacgtagg cgataatgta ggtgatgtag ccggtatggg tgccgattta tttggctcat
      301 atgtggctac cgtattagca tcgatggtgc ttggtaatta tgtaattcgc gattttgtgg
      361 aggcttatgg tagcattgcc aacattcctt tcagcggaat gggtcctata tttttaccac
      421 taagcattgc tgcaattgga attcttgctt ctatcgtagg aactttcttt gttaacatta
      481 gcgacaaaca agaagcaaat acaaacacag tacaatcggc tttaaacaga ggcaattgga
      541 tatcaattat actgtctttg gttggtgcat ttgtactaat aaaattgtgc ctgcccgaaa
      601 ctatgaccat gcgcatattt ggtaatggag aatttagcaa tatgactacc agtagcatga
      661 atgtgtttta tgccgttatg ataggaatgt ttgtaggcgg tgccatatca tacatcaccg
      721 aattttatac tgccataggc aaaggccctg taaatggaat agtacaaaaa tcgggaacgg
      781 gtgcagccac caatatcatt gcgggacttg gcaccggaat gttatccaca gccatgccgg
      841 ttattttatt tgccggtgct atttggggtg cttatgcact tgcaggattt tacggtgtag
      901 gtattgctgc ttcggcaatg atggctaata ctgctatgca acttgccatt gatgcttttg
      961 gccctattgc cgacaatgcc ggtggtatag ccgaaatgag ccacatgccc gaagacgtgc
     1021 gcgaaaaaac ggatattctt gattctgttg gaaatacaac tgctgctatt ggaaaaggat
     1081 ttgccattgc ctctgctgca cttacagctt tggctttgtt tgctgcatac gtaactttca
     1141 caggtataca aggtatcaat atattcgatg ctaaagtatt ggctgcatta tttattggag
     1201 gtatgattcc ggttgtattc agtgcacttg ctatgaactc tgtaggtaaa gccgcaatgg
     1261 atatggtaaa cgaagtacgc agacagtttc gcgaaattcc tggtataatg gaaggaaccg
     1321 gaatgcctga atacagtaag tgtgttgcca tttcgacaca agctgcctta cgcgaaatgt
     1381 tgttgccagg tgcaataacc attattaccc caatcattgt tggttnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnngtgcat atatggccgg tgttaccgtg agtggtgtag
     1501 tttgggctat atttcaaaac aatgccggtg gtgcctggga taatgcaaaa aaatcttttg
     1561 aaaaaggtgt gatgattaat ggcaaattgg aaaagaaagg ttcggatgca cacaaagctg
     1621 cagttgttgg tgatacagta ggcgatccgt ttaaagatac ttcgggccca tcgatgaaca
     1681 tattaattaa attgagttgt ctggtaggtt taacattggc tcctctttta ggccacaatg
     1741 aaagcaatgc cggtgcatgt tgcgaagaag atgcaaaatg tgagatggtt tgcgatatgg
     1801 aaaaatgcaa aagcatgact cctgagcaat gtgcagctta ttgcgacagc atgggatgca
     1861 gtgccgatga aaaaaataaa tgtatggaaa tgtgcaaggg caatgcttca atgccggcaa
     1921 cggcaatgcc aagtttggat gttgcaacta tgaaaggaat gtatataatc gatcaaagtc
     1981 actcatttgt tgattttaaa ttgcgtcact tacttaccga tgccaaaggt agccttgcta
     2041 ttgacacagc atattttgac ttgaccgatg acctcactac ctcaaaagta tttattcaac
     2101 ttgatgtaaa atctattgat acaaaaaata aagagcgcaa tgagcacttg cttaaagacg
     2161 acttttttga tgtagcgaaa tatccaaaaa tcacttttaa cggtaatagt atacgtaaga
     2221 gtgaaaaagc aaattatgct tatgttatta ccggtgattt gaaaatgaaa gatgtaacca
     2281 agcctgttga tttatatttt aactatatag gcaatattga aaatgagtat gctaaagagc
     2341 tagcgcattc atttgcaggt atgatggaaa ttaaacgcac cgattttaaa gttggcacgc
     2401 caagcacaat ggttggcgat aaagtggcaa ttgatttttc ggttgaaacc tatatgaaaa
     2461 aataataaac aaaatatttt tagaaaaagc gttgcaattg taacgctttt tttttgcctt
     2521 gataaatttc atttgtgatg atattctttg ctaaatgttt tcaatcaaat agaaaagaaa
     2581 aaccatatac ttaaaataac gggttctaaa taaaatcaaa gtgttgtcta tttatgtcaa
     2641 tactaaaatt gatttcaata tttggcaacc tgattttttt tctttgctag gtcaaaaact
     2701 atttttgctt tttgcttttc ttaaacacaa ctaccaataa gatgagcacc atagccggtg
     2761 atgagatttt aaaaagatat tcggtctttt ttgaaatggc aaatgccacc aacaaagccg
     2821 caatagcgat taagcaacaa acagttgcta ctgcgaatat tattcctctc aacttatcgt
     2881 tttcaattcc tgacataatt taaatccatg attaaaaaaa ttgaattaca cttttactgt
     2941 tttccattta ccttgtttaa atacaaaata acttatgatt gccatcacag tttcggaaaa
     3001 tggaatggcg ataaacactc ccagataatt taattgaaaa tgatgcgata acaaatacgc
     3061 caaaggaatt tgcaacaacc aaaatccgac aaaactgata atggttggtg tacgggtatc
     3121 gcctgcgcca ttaaacgaaa gcataagcac cattccaatg ccataaaaaa tgtatgccga
     3181 gcttatgtat tttaaagctt gcaccgcaat ggcaatttgt tcgggctgat tggcaggtgt
     3241 aaaaaaacga ataatccatt cgccacatgt gtaaaacaat agcgttacca tggccataaa
     3301 tatggcattg tactttgctg ttttcattac ggcttgttcg gcccgttgtg gttgttgtgc
     3361 gccaagattt tgacccacta atgttaccgc agcgttactc aatccccagg cgggcaggat
     3421 aaaaaatatt acaagacgta tggctgtttg atatcctgcc gatgcaatta cgcccgattg
     3481 tgcaactatt gctgcaagaa aaatccaact tgccgaagaa attaaaaatt gaaaaattgc
     3541 cggtgttgct atatcggcaa gtgccttttg cgatagccat tcaggaacaa aatcttctgt
     3601 ataaaaatga atgatgtgtt ttgcttttat caaaaaataa acctgataca atacaccaat
     3661 gccgcgccca atgcacgttg ccacagcggc accggtaatt cccatttgtg gaaacgggcc
     3721 atatccattt ataagcacag ggcataaaat aatattacaa atattagcaa cccacaaact
     3781 acgcattgct aaagctgcat cgcccgcacc acgaaatatt ccattgagca taaataataa
     3841 taccactact atgcttgttc caagttgtat gcgagtataa tttaatcctg cttctattgc
     3901 cgatggtgcc gcacccatga attgcaaaat atttttcgca aaaacgaaac cgattactcc
     3961 catcactaac gaaacaacaa cggccatcaa agttgcttgc gctgcggctt tactggcatc
     4021 gcgattattt ttttcgccaa tacgtctggc aactatggct gtggccgcac tgctcaatcc
     4081 tattgctacc gagtaaacaa ttgctgttac cgattcggtt aaaccaacta tggacacagc
     4141 accagggcca agcttgttta caaaaaataa atcgacaaca gcgaaaacac tttcgaggca
     4201 catttccagt atcataggaa tggccaaaag cacaactgcc ctgcgaatac ttattgttgt
     4261 aaaatcgtgt gcactactgt tgaaagcatc tttcagtagt tgtattattc tggtcaatgg
     4321 ttatttacta tggtgttgaa tggatttaaa gcattagcaa ccggattttt tctggcgata
     4381 gaagtcttca acattcacaa cctttccggc tttcataaag gactgattat aactcccctt
     4441 tcaaaaattt tccataaaat ctcttgttgc ccgattgtac ttcaacaaaa tatatccctc
     4501 gggttagcat ctccacattt attttttttg ggatttgctt ttcaaaattt tgttgcatgc
     4561 atttgttccc tttcacatcg tatatagtta tggttacatc atttgtaaat ggaagtttta
     4621 tctgcaaata gtttatggcc gggtttggat ataaacttag cgaaggcaat tcagaaaccg
     4681 caggtgtgcc taaacttaca atactatcca catcataagt catcatactg cgggcaaaag
     4741 tagcagcaca caaacggttc gagctataat caaaatctaa atcgtacacc ggtattatcg
     4801 gcatattatt tccaagtcga ttccaggtta caccaccatc aacagtagca taaactccgg
     4861 catcggtggc cacaaataga acatcatcac ctgcagcggt tacatataca tcattgattg
     4921 ctgcaggtgg caagttgccc gaaatatcga cccacgtatt tccattgttt atcgacttgt
     4981 gtatatgagg aataaaatcg ttgtacttgt agccagtgtg tgtaacaaat acggtgttgg
     5041 catttaatac agatgccttt accgatgata catatctatc gggcaataca cctgttacat
     5101 tttgccatgt tgtaccttgg tttgtgcttg cccacacatt gccatcggta gtgccggcat
     5161 ataaattatt atcgttggca ggcgactctt ctacacaaga aatggtgtga aacctatcgc
     5221 cataaataac gccatcggta atatcactac ttatcgaata ccagtttgca ttttctccat
     5281 catcgttgcg gtatacctga tatgtgcctg tgtacatgcg attgggatcg gctttgctca
     5341 tgatgtattg catatcccaa tgcctgcggt cgttaaagtc aattccattt gttgcatcat
     5401 taaaactatt gcctccatct gtcgacacat tgatacctcc attttgatat tcggcccaca
     5461 taatattggg atcggtaggg tgatataaag gctgaaaacc atcggcacca aatacacgta
     5521 tccaattatt gaatgacgct gcattgccat attctgttcc attatcctgt gcaccacctg
     5581 cgtatactcc ttgtgcatgc gggtttacag cgcatctata aaattgtgtg ttgggaatgt
     5641 tttcaatatc attccatgtg ctgccaccat tagtagtttt gtacaagcct ccatctgtgg
     5701 cacataattg tacactattc gataaatatc ttatatcatg tttgtcggca tgcatatcat
     5761 aagccgggtc aacatcataa aagttatcgc caccatcggt cgatttgtgt aaaccaattg
     5821 cacaaaaata tagttcgtta tcgttggtgg gatttatttc gatacggcca aagtaccaac
     5881 cgaaaccgcc ctgcacaccc aataaattat tgccgccttt ttgtgtccac gatgttccgc
     5941 catttgtagt tttataaata ccaagcaaat caaaatttac atctacatac gaagcataaa
     6001 ttacattggc gtttgtacca ctcatggtaa ggccaatacg cgaggcatct attattggca
     6061 atccattggt gagcatattc caggtagtgc caccatctgt tgttttataa atgcgtgcat
     6121 cgggaccaga aattaaactt cgctgattac tgcgcaaacg attccagccg gcagcataaa
     6181 gcacatcagg gttttgaggg tccatcaaca tatcaatcac accggcacta tcgcttatga
     6241 aaagtgattg ctgccaagtt acaccaccat caatggattt gtatacgcct cgattattat
     6301 cttttataaa aggtacaccc atagttgcgg cataaattat attggggttt gtagtgtgga
     6361 tttttatttt ggtgatgata cgtgtgttgg caagccccat atttgtccat gtattgccgc
     6421 catcggtgct tttataaatg ccatcgccaa taaaaggata accgcttatt tgagcatcgc
     6481 cagttcccac ccacatagta tttggattgc ccggttcaaa ttcgatacaa cttactgaaa
     6541 gtgccgattg atcatcaaaa atcggattcc atgttacacc gccatctgtt gttttatgaa
     6601 ttccacctgt ggcattgccc acatacatta tcatattatt tgtgggatgc atggccacac
     6661 aatttattct gccaccaata ttgcccgggc cttccattgt ccatgcattg ttgaaaccca
     6721 ctgtgttagc cagtttcgat tgcaacgatt gttgctttgt tatttgcatt gcttgctcgt
     6781 atccttttac atccatagta aaatcgggat agtttcgcat cataaaaaaa tcttcggcag
     6841 gcaatgcgct aatttttgga gttggagtgt tgtgcttctt tgtaaaaact aatatggctg
     6901 aagctactgc aatggcagat aaaacaataa aggatatttt tttcatgacg attttttatt
     6961 tcattaaaac gaaatcaaat taacataatc taaaatgaaa caacaatatt ttagcaagca
     7021 agttcaaaaa aaatgtgaca cttttaatac acagaacaat gattaatttt ttctgctttt
     7081 ttgatattgt acaatcatta
//
LOCUS       DAORQA010000079         6935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2799_length_6935_cov_10.447529, whole genome shotgun sequence.
ACCESSION   DAORQA010000079 DAORQA010000000
VERSION     DAORQA010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2799_length_6935_cov_10.447529"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..446
                     /locus_tag="PK736_05635"
     CDS             204..446
                     /locus_tag="PK736_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32905.1"
                     /translation="MKNSNWFKKVGLIFVPVSIVGAILLLLTVAFCVTVFVAVDRQSH
                     SVTDTLYGIFPSFVSAFTVLFWVANNTSVESRTSLK"
     gene            539..1174
                     /locus_tag="PK736_05640"
     CDS             539..1174
                     /locus_tag="PK736_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32906.1"
                     /translation="MESQEIIPQQNKFDRAFKNEIRAGRILCYINSVVSIVAAIPAMF
                     CVLATVFCFIDTLPSTFHHPDSFERPMDMKTTLWFACASIILMLYYRFIFNNPFYKKE
                     PDTISVRFTYWWQLALLCSATLFLLIGTFKMFYFTVLFEPLNMPIAGRIFLRAIILFA
                     YSPIIIFVLALFGLYNNINQHDGKAFYNTNSNQTSHTVKQNAPITNTTQTE"
     gene            1206..4013
                     /locus_tag="PK736_05645"
     CDS             1206..4013
                     /locus_tag="PK736_05645"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HRC32907.1"
                     /translation="MKKNDWILLFSLAVYSVLFYAQSFGINFLLFSVFVCIMLFVRNK
                     ELIKNKTWNIVALCTIASGFAVFVYGNYLSFVANVISLSILSALSFNQQTSILLGLLY
                     AVYSYVGAIAYMFKDYAERKKVEVEKSHPMAIKILLVVIPVLIVTVFFLLYQSSNPVF
                     YNFTQKINFDFISLAFIRFVIIGFILLYGFYYHRTINIFQTADEQAANNLRKENAVYK
                     SFFGNGFTIEYENISGQILLGLLNVLLLTVNLLDIIYLWGTGGLPQGMSYAAFLHQGT
                     GTLITSIVLAVSIILFYFRGALNYFEKNKSLRILAFVWVLQNIFMVISTAYRNHIYIE
                     EYFLTYKRIGVYVYLFLCVIGLVTTFIKIKNIKSNWYLFRVNSWWCYALLIACSFVSW
                     DLLITKFNVSTATEKNKPIDKNYLIGLGYINLPDLILMNDSLQNKASEEENDMISFSS
                     EYERPNRFSSYAYLPGDNFKSEINYKLYTFLDNVENHDWQSFCGMKHEQYNALFMLGG
                     QKKITTIDISNRSIESLMPLKNIKHISKLDVSKNRIKNIDELKYFTEVEDLNIAQNNF
                     DTIASVPLLPKLKTLNISSNKIYPLHKLSSWNTIEKLTAIEINSRELKGLPTFPKLRE
                     LDLSSNNLANIPPLDKYPLLEKVALRNCVSIEEYEFPLMPSIKSIDLSNNGFNLQKNF
                     VLDNLLLLPNVQEVDLSNNNMIYIPKASSNVAELNKQTFFDGITYLNINNNRIDDLYG
                     IEKFISLNNLIISSNKLWTLNGIQNSPQLKHLSINQNEFLTDIKAIQNLTSLQSLEMG
                     GCFRVHNWEYLKSLSQLQSLSVSGCGLKKMNFLNSQNQLTSLDCSNNNLTDLSFLFQL
                     QNLNELNLSENKIENLAPLYSLKKLKRLIIRKVTSPEEITKLQKMLPNTEIICEIFGN
                     ENIDSQIVDAVMPATTNNNGE"
     gene            4010..4435
                     /locus_tag="PK736_05650"
     CDS             4010..4435
                     /locus_tag="PK736_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32908.1"
                     /translation="MMTPFFKAAAITCFTCFLYVIPISLTTIFLCHDLFQFNIDLHIV
                     LMLVLGCISCITLNVIINPLIYLFDNKAMMTQTSTRLFHRYMPIYAAPYSIIFAIVFY
                     EGGIDKEFLVHVFSIMVISYANLIAFVKNICVVKKEELK"
     gene            complement(4451..4927)
                     /locus_tag="PK736_05655"
     CDS             complement(4451..4927)
                     /locus_tag="PK736_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRC32909.1"
                     /translation="MGKTHAYNTTIVWTGNKGKGTADYKAYDRDFILKINNKPDILCS
                     SDIPFLGDGTRHNPEDMLLAALSSCHMLWYLHICADKGITVTAYEDNATGTMIQKPTG
                     GGNFIEATLNPTITITDAAHIELATALHTDANKNCFIANSCNFPVHHKPQFVIANN"
     gene            complement(4998..6278)
                     /locus_tag="PK736_05660"
     CDS             complement(4998..6278)
                     /locus_tag="PK736_05660"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRC32910.1"
                     /translation="MKTKQLLLSLIAFFAISCANAQTWTNLNSGSASDLKAIHFPTTN
                     VGYAVGTLGTILKTTNSGTTWQNTTVNYPGYWFWDVHFLNADTGFVVGESDPGFNPNG
                     AGIILKTTNGGTTWNAVVMNAPNPFRDLFVLDANTIFACGGAEQISTALLKSTDGGTT
                     WNSLGLNYVDATMGGMYFINNNVGFVAMYESVFGSVNPTSTTWFTTTNGGTVFTPLVI
                     PNSISYWNFATDFGDATTGYSTRATYSGIDLVYIKKSTDGGNTWSEKSIPNFIGSIYG
                     MDFLAADTGYIVGAAGVIFKTIDGGNTWLTEVSGTFNELRSVHFVNSALGFAAGANGT
                     ILKWTSSSIGINENNISVSGNVYPNPNSGIFTLNNNESNSTIEIFNTQGQLVFTQQLV
                     FSYHDFNISTFAAGVYTIVVTSGNQVERIKLVKE"
     gene            complement(6391..6891)
                     /locus_tag="PK736_05665"
     CDS             complement(6391..6891)
                     /locus_tag="PK736_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRC32911.1"
                     /translation="MKATINNTEFDVEKSDDGLTYMVNNKLNDADIQPLGNNTFHILW
                     HNKGYRAEVMTIDRTEKMVTMKINGQQYEVKLSDRFDVLLHEMGLDKLVTKKVLDIKA
                     PMPGLVVKVLVEKGAQVNEGDSLLILEAMKMENIIKAAATATVTSVNIKPGQKVEKGE
                     LLIGLS"
BASE COUNT         2159 a         1265 c         1187 g         2324 t
ORIGIN      
        1 atcagatcgc gaagagcaat ttcttgcacg aattgatgcc ggtgaaatta tcaaaagctt
       61 gtgatttttt ttagggtcgc aactatgagc aggaggcgaa gcggttgtta aatttttaaa
      121 aaaatatttt ggaaagtaaa aacaattcat ttgctttgca gcacaaagta cttttgtttt
      181 tatcaattta aaacttcttc attatgaaaa attccaattg gtttaaaaaa gtaggattga
      241 ttttcgttcc tgtttctatt gtaggtgcaa ttttattgct gctaactgtt gcgttctgtg
      301 tcaccgtgtt tgttgctgtc gacaggcaat cgcatagcgt caccgatact ttatatggaa
      361 tttttccttc gttcgtttct gcatttactg ttttattttg ggtggcaaat aatacttcag
      421 tagaaagcag aacatcactc aaataataaa aatttaaaat caaacacaac atcggagtca
      481 taagctccgt taatttttga acattcactt tgtatttcaa agcactctga ataaaattat
      541 ggaatcacaa gaaatcatcc cacaacaaaa taaatttgac cgcgcattca aaaatgaaat
      601 ccgtgccggt cgcatattat gttacatcaa ttctgttgta tcaattgtag cagccatccc
      661 ggctatgttt tgtgtattag ccactgtatt ttgttttata gatactcttc cttctacttt
      721 tcatcatccc gattcgtttg agaggcccat ggatatgaaa actactttgt ggtttgcttg
      781 tgcctcaata attttgatgt tatattatcg tttcattttc aataatccat tttataaaaa
      841 agaacccgac accatcagtg tgcgctttac ctattggtgg cagttggcat tactttgctc
      901 ggccactttg ttcctgctaa ttggtacttt taagatgttc tatttcactg ttctattcga
      961 gccattgaac atgcccatag ccggcagaat atttttgaga gctataatcc tttttgctta
     1021 cagcccaatt atcatattcg tgcttgcact atttgggcta tataacaaca taaatcaaca
     1081 tgatggaaaa gcattttaca atactaacag caaccagaca tcgcacacag taaaacaaaa
     1141 tgcccctatt acaaatacaa cacaaacaga ataataatca aactaaaaat ctcaaaaata
     1201 tatttatgaa aaaaaacgat tggattctcc tgttctcttt agctgtttat agtgttttat
     1261 tttatgcgca aagtttcggt atcaactttt tactattctc agttttcgtg tgtatcatgc
     1321 tttttgttcg caacaaagag ttgataaaaa acaaaacgtg gaatatagtt gcactatgca
     1381 ctatcgcctc gggttttgcc gtatttgtat atggcaatta tttaagcttt gtagcaaatg
     1441 taatttcgct cagtattttg tcggctttaa gtttcaatca gcaaacctct atcctgttag
     1501 gtttactata tgctgtttac agttatgtgg gtgctatagc atatatgttt aaggattatg
     1561 ccgagcgcaa aaaagtagaa gttgagaaaa gccatcccat ggcaattaaa attttgctgg
     1621 ttgttattcc tgttttaatc gtcaccgttt tctttttgct ttatcaatct tccaatccgg
     1681 tgttttacaa ttttacacaa aaaataaatt tcgattttat ttcacttgct ttcattcggt
     1741 ttgtaattat aggtttcatt ttgttgtatg gtttttatta tcaccgcact ataaatattt
     1801 ttcaaacagc cgatgagcaa gcggccaata atcttagaaa agaaaatgct gtatacaaaa
     1861 gtttttttgg taacgggttt actatcgaat acgaaaatat ttcgggccaa attttattag
     1921 gcctactcaa tgtattactg ctaaccgtaa atcttctaga cattatttat ttgtggggta
     1981 caggcggatt gccgcaaggc atgagttatg cagcgttttt acatcaaggc acgggaacac
     2041 ttatcacttc gatcgtactt gccgtctcca ttattctttt ctacttccgt ggtgcactca
     2101 attatttcga aaaaaataaa tcgctgcgga tacttgcttt tgtctgggtt ttacaaaata
     2161 ttttcatggt aatctctacc gcctatcgca atcacattta tatcgaagaa tattttctca
     2221 cttacaagcg cattggagtc tatgtttatt tgtttttatg tgtaatagga ttggtaacaa
     2281 cttttataaa aataaaaaat ataaaaagca attggtattt gtttcgtgta aatagttggt
     2341 ggtgctatgc tcttttaatt gcatgttcat ttgtaagttg ggatttacta ataactaagt
     2401 ttaatgtttc tactgcaaca gaaaaaaaca aacccatcga taaaaactac ttgattggat
     2461 tgggttacat taacctaccc gacttaatat taatgaacga tagtcttcaa aataaagcat
     2521 cggaagaaga aaatgacatg atttcattta gttcagaata tgagcggcca aacagattta
     2581 gtagttatgc ttatctgccg ggagataatt ttaaaagtga aattaactat aagctgtata
     2641 cgtttcttga taatgtggag aaccacgact ggcaatcgtt ttgcggaatg aaacacgagc
     2701 agtacaatgc cctgtttatg cttggcggtc aaaagaaaat taccaccatc gatataagta
     2761 acaggagtat tgaaagcctt atgccattga aaaacataaa gcacattagc aaactggatg
     2821 taagcaaaaa cagaattaaa aatattgatg agttaaaata ttttactgaa gttgaagatt
     2881 taaatattgc tcaaaacaat ttcgatacca ttgcctcggt tccattgtta cccaaattaa
     2941 aaacgctaaa tatttcttca aacaaaattt atccgctgca caaattatcc tcgtggaata
     3001 caattgaaaa gttaacagca atagaaatta attctcgcga attaaaaggg ctgcccacat
     3061 ttccgaagtt acgtgagctt gatttatctt cgaataattt agcaaacatt ccaccgcttg
     3121 ataaatatcc gttgctcgaa aaagttgcgc tgcgcaattg cgtgagtata gaagaatacg
     3181 agttcccgtt aatgccatcc ataaaaagta ttgacctcag caacaatggt ttcaatcttc
     3241 aaaagaattt tgttcttgat aatttattgt tgctgcccaa tgtgcaagaa gttgacctta
     3301 gcaataacaa tatgatttat attccaaaag caagtagcaa tgtggctgag ttgaataagc
     3361 aaactttttt tgatggcatc acttatctga atataaacaa taatcgtatt gatgatttgt
     3421 atggaataga aaagtttatc tcgctaaata atcttatcat ttcatcgaac aaattgtgga
     3481 cgttgaatgg aattcaaaac agcccacaac tcaaacacct cagcataaat caaaacgaat
     3541 tcctgacaga tataaaggcc attcaaaatt taacttcgct gcaatcgctt gaaatgggcg
     3601 gatgtttcag agtgcacaat tgggagtact tgaaaagtct ttcgcaattg caatcgcttt
     3661 cggtatcggg atgtggactc aaaaagatga attttttaaa tagtcaaaat cagttaacgt
     3721 cactcgattg cagtaataac aatttgactg atttgagttt tctgtttcag ttacaaaatc
     3781 ttaatgagtt gaatttatcg gaaaataaaa ttgaaaacct tgcaccattg tatagtctga
     3841 aaaaactaaa acgattgatc attcgaaaag tgacgagccc cgaagaaatt acaaaattgc
     3901 aaaagatgct gccaaatacc gaaatcattt gcgaaatttt tggcaacgaa aatattgata
     3961 gtcaaatagt agatgctgtg atgcctgcaa ctacaaataa caatggcgaa tgatgacacc
     4021 atttttcaaa gctgcagcca ttacatgttt cacatgcttt ctgtatgtga ttccaatatc
     4081 gttgaccaca atttttttgt gtcacgattt atttcaattc aatattgatt tgcatatcgt
     4141 tttaatgttg gttcttggtt gcattagttg cattacatta aatgttatta tcaatcctct
     4201 catttattta tttgataaca aagcaatgat gacccaaaca tcaacacggc ttttccatcg
     4261 atacatgccc atttacgcag caccgtattc aattattttt gccattgttt tttatgaagg
     4321 tggtattgat aaagaatttc ttgtgcatgt tttttctatc atggtaatat catacgcaaa
     4381 cttaatcgca tttgtaaaaa acatttgcgt tgttaaaaag gaggaattaa aatgacaaag
     4441 aaatcagttt ttagttattc gcaattacaa actgaggctt gtggtgcaca ggaaaattac
     4501 acgagttggc aataaaacaa tttttgtttg catcggtatg caatgctgtt gcaagttcta
     4561 tatgtgctgc atcggtaata gtaattgtag gattcaacgt tgcttcaata aaatttcctc
     4621 caccggtagg tttttgtatc atggtgcctg tggcgttgtc ttcatatgca gttactgtaa
     4681 ttcctttgtc ggcacaaatg tgtaggtacc aaagcatatg gcaacttgat aatgctgcaa
     4741 gcaacatgtc ttcgggattg tgacgcgtgc catcgccaag aaaaggaata tcggacgaac
     4801 acaaaatgtc gggcttgtta tttattttca aaataaaatc gcggtcgtag gctttataat
     4861 cagcagtacc cttgccttta ttacctgtcc acactatggt ggtattatat gcatgtgttt
     4921 ttcccatttt ttttattttg aagttttaga agttcgaaat atgggaaaat attacatgca
     4981 aaaactgcga tgacgaatta ctccttcaca agttttatcc gctctacttg gtttccgctt
     5041 gtaaccacaa tggtatacac tccggcagca aaagttgaaa tattaaaatc atgataacta
     5101 aaaactaact gttgcgtaaa caccaattgg ccttgtgtat taaatatttc tatggtgcta
     5161 ttgctttcat tattgtttaa agtgaaaatt cctgagttgg ggtttggata tacgttgccc
     5221 gatacagaaa tattattttc gtttatgcca atggaagagg aagtccattt aagaattgta
     5281 ccatttgcac cggcagcaaa accaagagca ctgttgacaa aatgtactga gcgcagttcg
     5341 ttaaacgtac ccgaaacctc tgtcaaccat gtgtttccgc catctatcgt tttaaagata
     5401 actccagcag cacctacaat gtatcctgta tcggctgcta aaaaatccat accataaatg
     5461 ctgccaataa aattggggat acttttctcg ctccacgtgt ttcctccatc ggtggatttt
     5521 tttatgtaaa ccaaatcgat gcccgaataa gttgctcgtg tagaataacc cgtagttgca
     5581 tcgccaaagt cggttgcaaa attccagtac gatatactgt taggaataac cagaggagtg
     5641 aaaaccgtac cgccattagt agtagtaaac catgttgttg aagtaggatt tacagagcca
     5701 aatactgatt catacatagc cacaaaacca acattgttat ttataaaata cattccaccc
     5761 atagtagcat caacataatt gagccccaaa ctattccatg ttgttccacc atctgtggat
     5821 ttaagtaatg ctgtgcttat ttgttcagca ccaccgcaag caaatattgt attggcatcc
     5881 aacacaaaaa gatcgcgaaa cggatttggt gcattcatca caacggcatt ccaagttgtg
     5941 ccgccatttg tagttttcaa aataataccg gcaccattgg ggttaaatcc cggatcgctt
     6001 tcaccaacaa caaatccggt atcggcattc aaaaaatgca catcccaaaa ccagtagccg
     6061 ggataattta cagttgtatt ttgccatgtg gtgccactgt ttgtggtttt taaaatggtg
     6121 cccaaagtac ctacagcata gccaacatta gtagtaggaa aatgtatggc ctttaaatcg
     6181 ctcgcactac cgctattcaa atttgtccat gtttgagcat ttgcacacga tattgcgaag
     6241 aatgcaatta atgataatag taattgtttt gttttcattt ttattgtttg ttaatcatca
     6301 atacgtttca atattttaat atgtgagtgt gattaaaaaa aattgcagaa ataaaaactg
     6361 catcaaaaaa tattttggct caaggcaaag ctaactcaat ccaatcaaca actcgccctt
     6421 ctctactttt tgtccgggtt ttatatttac acttgtaacg gttgctgtag ccgcagcttt
     6481 aattatattt tccattttca tggcttcgag tatcaatagc gaatcgccct cgtttacctg
     6541 cgcgcctttt tcaactaaaa cttttacaac aagacccggc ataggcgctt ttatatcgag
     6601 cactttttta gttactaatt tatctaatcc catttcgtgc aacaatacat cgaagcggtc
     6661 gctcaatttt acttcgtact gttgtccgtt tatcttcatg gtaaccattt tttcggtgcg
     6721 gtctattgtc atcacttcgg cacggtagcc tttgttgtgc cataggatat gaaatgtatt
     6781 gtttcccaga ggttgtatat cagcatcatt cagcttgtta tttaccatgt atgtcaatcc
     6841 atcatcgctt ttctcaacat caaattccgt attatttatt gtagccttca ttttcaaaat
     6901 tttatgcaat attaattatt cactcgatat ataca
//
LOCUS       DAORQA010000080         6894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2834_length_6894_cov_14.191548, whole genome shotgun sequence.
ACCESSION   DAORQA010000080 DAORQA010000000
VERSION     DAORQA010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2834_length_6894_cov_14.191548"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..803)
                     /locus_tag="PK736_05670"
     CDS             complement(243..803)
                     /locus_tag="PK736_05670"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRC32912.1"
                     /translation="MRKLGFGFIAVAIAFLGYSFMSVPQNKTASVVTGIEVGNKAVDL
                     KFMSPEGKEIALSSLRGKIVLLDFWASWCRPCRMENPNVVAAYQKYKDTKFNAKAKGF
                     TVFNVSLDQNKDQWVKAIATDKLDWPNHVSDLGGWQSKPAAVYQVQSIPTNFLINENG
                     IIVGRDLRGPALEQALEKLITTRKGK"
     gene            complement(922..3426)
                     /locus_tag="PK736_05675"
     CDS             complement(922..3426)
                     /locus_tag="PK736_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32913.1"
                     /translation="MKKTLTLFLISIASMAMAQESALTPPSIIKGPWKEKMLRQRWYY
                     PYGNATPEYLQRAADEIKNMPDEQSLSNGKVATVNAWENIGPFGIIPGSTIVYSGRVK
                     DVEYTKQGTLRILSATGGLWQIDSLSNGTIVNQSLSDHLDCNSGGAFATDPNNSDVIF
                     LGTGEGALSGGCGLWKTTDKGVTWTKLTLTPQPSTFYELIIINGNPLKLYAAANTGLY
                     QSDDGGVTWTRTKQGEITDVVVNPTNPNNIVLAQWNSGIYRSIDGGLNFIKNTSAPGT
                     NVGRTALDIALSSPNIIYSNITENTSNGTKGIYKSVDDGATWTKCVLGNDINGNPANS
                     NFHGNQGWYDNSIGVNPTDPNIVMAGGVSIFRTTDGNIFNEVDGRHADQHAVSFAANN
                     WCIIGNDGGVFISKNNGFSFNTINTATNNLPITQYIDCSASKASASSMLGGTQDNGTV
                     YRSTNVPNWTTTGGDGGGVASDPFFPLNLYTAVGVYGGTHAFHRQFSANGGMSWQDNT
                     TGIDPNGQWYPALRLDNNTGALYTNASSYLYRTFDFGVTWEKVNPNNAFTSEIYSFSI
                     DQSGSSEPSIYTLVNSKVKVRDRNLLQWVDRTGTLPNLGFNQIQADFVLPDRVFATVT
                     DISAAAAGNKVFRSDDIGLTWTNISGNLPNVPITDILPSPINANIYYVATTEGGFKTL
                     DGGITWVKWANGLPKRVEMSKLDYIDSTSINGKFYVLASTYGRGLWLRDVSGDDPLSV
                     FNQHQNYNSGVTLVQVKPNPALENSVISFHLSSSTNVNLSLYDFSGKLIKTITNREFS
                     KGVNQQLINLQDVEAGVYTLRISTAEGQATQKIIKL"
     gene            3693..5228
                     /locus_tag="PK736_05680"
     CDS             3693..5228
                     /locus_tag="PK736_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HRC32914.1"
                     /translation="MANTETVKTGHPKGLYFLFFTEMWERFSYYGMRAIFILFMTKIL
                     MMKDADASQIYGSYTGLVYLTPLLGGYLCDKYLGNRRSIILGGLLMAFGQFCMFLSAS
                     AGADGGVPIMWAGLTAIIIGNGFFKPNISTMVGQLYPEGDRRIDSAFTIFYMGINLGA
                     FFSPLVCGSMDFKWGFLAACVGMIISLISFVMFQKSHLISEEGKEIGLSVQKLDLKSI
                     LMIVGSIAIVYFMLNFKQMFKNDTDIISYFIYGSMILMPILILSDKSLTKLERNRIIV
                     IFILAFFVIFFWGAFEQAGASLTLFADRQTERTIFGHEMPASYFQSINPLAVICLAPV
                     FTLIWGFLYTRKLEPSSPYKMAYGLALVAVGYIVIAIAVKGLGIDEKVSMWWLVALYI
                     IHSMGELCLSPIGLSMVSKLAPLRLSSLMMGTWFLANAAANKFAGTLSALIPPSGPAV
                     AGAVVEYPSFLGFKITNLYEFFMLFIVMTGAAAAILFVLSSWLQKMMNEKHNEYREEL
                     DRF"
     gene            complement(5326..6123)
                     /locus_tag="PK736_05685"
     CDS             complement(5326..6123)
                     /locus_tag="PK736_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HRC32915.1"
                     /translation="MKKIVVIFCFTLLSKGTFAQRVKTVLPNSKGNTIVKSKMVWSEE
                     FNLNNLDTIAWKVNTGEHGSEEQYYRNSKKNIFIENGILKLTAHKEKHERFNYSSGQI
                     ISKKEFSFGYYEVRAKVPSGKGLWPAFWFYKGSGTYYQEIDVFEFGGSNTSRYWVNNF
                     WKAATGDLSDNAMIIPKLNGWWFFNKKLDLSDDFFTYGVNLAPNKIEYYFNGQLVHVK
                     TTHVPQLPMPVFLNLAVGGFYDGPPNAQTVFPCSFEIDYFRAYENLK"
     gene            6355..>6894
                     /locus_tag="PK736_05690"
     CDS             6355..>6894
                     /locus_tag="PK736_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32916.1"
                     /translation="MKKIFLTLALFGACITFALAQETKDDKINKMIKDGAEYVKAKQY
                     KEAAGSLQEAINIINEIIGDVILQGLPKSAGGLTSDPANDQITSLGTLMGAGMQVQRM
                     YYNEKGQSVNITIQPNSPQLSSLNTFLDNPDDYPGEEAGKRVKVKDFKSLLRFTKAEG
                     EESSSSGYLTMPFYTSVVTV"
BASE COUNT         2091 a         1256 c         1293 g         2254 t
ORIGIN      
        1 ataaaataga tgctttctat tttataaata caattcatat aaaagcaaag cctggattgc
       61 aaattgattt acaaacgaaa agcagggaga gcaatatttg attttggttg tagagtctgg
      121 aatcaaacat gtggaaaaaa acgaattacg gtacgactat aaaaaggatg gattgataga
      181 aaaagaaaaa ccggatgact ttttcaaatc atccggttaa aaataataat tacccctaat
      241 ttttattttc cttttcttgt tgttattaac ttttccaggg cctgttccaa agctggacct
      301 cttaagtctc taccaacaat tataccattt tcgtttatta aaaaattagt aggaatggac
      361 tgcacttggt aaactgcagc aggtttcgat tgccatccgc ccagatcgct tacgtgatta
      421 ggccaatcaa gtttgtcggt tgctatagcc ttaacccatt ggtctttatt ttggtctaat
      481 gacacgttga atacagtaaa tccctttgct ttagcattaa acttggtgtc tttatatttt
      541 tgataagcag caactacatt ggggttttcc attctgcatg ggcggcacca cgatgcccaa
      601 aagtccagca aaacaatttt acctcttaaa gatgacaaag ctatttcttt gccttcgggg
      661 ctcataaatt tcaaatcaac agctttgttg ccaacttcaa tgccggtaac aaccgatgca
      721 gttttatttt gaggaacact cataaagctg tagcctaaaa aagcgatagc aactgcgata
      781 aaaccgaaac ctaattttct catatttttt ttagtttata aatttaaagg gccaaagata
      841 atgccgtatt cttaatccaa aatatttttg cctatcaatt aatgtaaact acattattag
      901 ctttttgaaa agagaaacga actataattt tattattttt tgtgttgctt gtccttctgc
      961 tgtcgaaatt ctaagagtat acactccggc ttccacatct tgtaaattaa ttaattgctg
     1021 attgacaccc tttgaaaatt ctctattggt aattgttttt attaattttc cagagaagtc
     1081 atataatgat aaattgacat ttgttgatga cgaaagatga aaacttataa cactattttc
     1141 aagtgccgga ttaggtttaa cttgcaccaa agttaccccg ctgttatagt tttgatgttg
     1201 attaaaaacc gataaaggat catcacccga aacatcgcgc aaccataatc cgcgaccata
     1261 tgttgacgcc aaaacataga actttccatt tattgatgtg ctatcaatat aatcaagttt
     1321 gctcatctca acccgctttg gtaatccatt cgcccacttt acccaagtga tgccgccatc
     1381 aagtgtttta aagcctccct cggttgttgc aacataatat atgtttgcgt taatgggaga
     1441 tggcagtata tctgtgatag gcacattggg taaatttccg cttatgtttg tccatgtcaa
     1501 gccaatatca tctgaacgaa atactttgtt acctgctgcg gctgcgctaa tgtctgtcac
     1561 agtggcaaat actctatcgg gcaacacaaa gtcagcctga atctgattga atcctaaatt
     1621 gggtagcgtt ccggttcggt caacccattg caataaattt ctatcgcgta cttttacttt
     1681 cgagttgaca agggtgtaaa tgcttggttc gctagaaccg ctttgatcaa tactgaaact
     1741 ataaatttcg gaagtaaagg cattgtttgg gttaaccttt tcccaggtta caccaaaatc
     1801 gaaagtacga taaagataac tgcttgcatt tgtataaagc gcacccgtgt tattatctaa
     1861 tcttaatgca ggataccatt gtccgttagg gtcgatgccc gttgtattat cttgccacga
     1921 catgccaccg ttggcgctaa attgcctgtg aaatgcgtga gtacctccat aaacaccaac
     1981 agcagtataa agattcaatg gaaagaaagg atcagaagca acaccgcctc catcgcctcc
     2041 ggttgtagtc cagttgggca cattggttga tcgatatacg gtgccattgt cctgagttcc
     2101 gccaagcata gaacttgccg aagccttcga tgctgaacaa tcaatatatt gcgtaatagg
     2161 taaattattg gtcgcagtat ttattgtatt aaaagagaag ccattatttt tcgaaataaa
     2221 aacgccacca tcgttaccaa ttatgcacca attatttgca gcaaaagata cagcatgctg
     2281 atcggcatgg cggccatcta cttcattaaa aatatttcca tcggtggtac gaaatataga
     2341 aacaccaccg gccattacaa tattgggatc ggttggatta acgcctatcg aattatcata
     2401 ccatccttga ttaccatgaa aatttgaatt tgcaggattt ccatttatat cattaccaag
     2461 cacacatttt gtccatgtgg caccatcgtc tacagatttg taaattcctt ttgtgccatt
     2521 gcttgtgttt tcggtaatgt ttgaatatat aatgttaggt gatgatagcg caatgtcaag
     2581 ggctgttcgt cctacatttg tacctggtgc cgaagtgttt ttaataaaat ttaaaccacc
     2641 atcgatagac ctgtaaatgc ccgaattcca ttgtgccaaa acaatgttat tcgggttggt
     2701 cggattaaca accacatcag taatttcacc ttgctttgtt cttgtccatg ttacgcctcc
     2761 atcatccgac tgataaagtc ctgtattagc tgcggcatat aatttcaaag gattgccatt
     2821 tattattatc aattcataaa aagtactggg ctgtggtgtg agtgttaatt ttgtccaagt
     2881 cacaccttta tcggtagttt tccaaagtcc acaacctccc gataatgcgc cttcacccgt
     2941 accaagaaat atcacatcag agttgtttgg gtcggtagca aaagcgcctc cggaattgca
     3001 atcgaggtgg tcgctcaacg attgatttac aattgttcca tttgagagtg aatctatttg
     3061 ccacaaacca cctgtggcac ttaatattcg cagggtccct tgtttggtat attctacatc
     3121 tttaacccgg cccgagtaaa ctatagtaga ccctggtata ataccaaaag ggccaatatt
     3181 ttcccatgca tttactgtgg caacttttcc attagaaagg ctttgctcat cgggcatgtt
     3241 ttttatttca tctgcagcac gttgcaaata ttcaggtgta gcattgccgt atggataata
     3301 ccaacgttgg cggagcattt tttctttcca agggccttta attattgaag gtggtgtgag
     3361 cgcactttcc tgcgccattg ccattgaagc aattgagatt aaaaaaagag taagcgtttt
     3421 tttcatcgat attttttgct tttagttttt aagaaatgaa atccaaataa acacattgtt
     3481 tgtaaagtat agtacaaaaa tgtcacaaca tagtttcaat tggaattcaa atatatgtca
     3541 tttacttgta aagaatttct aaaaaataat atttgataaa acgttaacaa aattttcgaa
     3601 ttcaaagaaa taatttcacc atcgtaaaaa atatatactt tagccaacga aacaaaatca
     3661 atataaattt tctaaacctc taaaatcaaa ttatggcgaa taccgaaaca gttaagacag
     3721 gacatccaaa aggtttgtac ttcttatttt tcacagagat gtgggaacga tttagttatt
     3781 acggaatgag agcaatattc attctgttta tgactaaaat attaatgatg aaagatgccg
     3841 atgcatcgca aatttatggt agttatacgg gtcttgttta tttgacacct ttacttggag
     3901 gttatttatg tgataaatat ttgggtaacc gcagaagtat tattttgggt ggattgctta
     3961 tggcttttgg acaattttgt atgtttctaa gtgcatcggc cggtgccgat ggtggtgtgc
     4021 caataatgtg ggccgggctt acagcaatta ttattggtaa tggatttttt aaacccaata
     4081 tatcaacaat ggttggtcag ttatatccag aaggagacag aaggatagat agtgcgttta
     4141 ccatctttta tatgggtata aatttagggg cattcttttc acctttagtg tgcggttcaa
     4201 tggattttaa atggggattt ttggcggcat gtgttggtat gattatatcc cttatcagtt
     4261 ttgtgatgtt tcaaaaatct catctcattt cggaagaagg caaagaaatt ggattgtcgg
     4321 ttcagaaact agatttgaaa agcatattga tgatagtggg gtctatcgca atagtttatt
     4381 ttatgctcaa ctttaaacaa atgtttaaga acgatacaga tattataagc tactttattt
     4441 atggttcaat gatactgatg cctattttaa ttttgagcga taaaagtctt acaaagcttg
     4501 aacgcaatcg aataatagta attttcattt tggcattttt tgtaattttc ttttggggag
     4561 cttttgagca agctggcgca tcattaactt tgtttgcaga tcgccaaact gagagaacaa
     4621 tatttggcca cgagatgccg gcttcctatt ttcaatccat aaacccattg gccgttattt
     4681 gtcttgctcc tgtgtttacc cttatttggg gtttcttgta cactagaaaa cttgagccat
     4741 cttcgcctta caaaatggcc tatggtttag cactggtagc cgtaggttat attgtaattg
     4801 caattgctgt gaagggttta ggtatagatg aaaaagtaag catgtggtgg ttggttgctt
     4861 tgtatattat tcattcaatg ggtgaattat gtttatcgcc aattggcctt tctatggtat
     4921 ctaaacttgc acccttgcgt ttgtcatcgt tgatgatggg cacctggttt ttagcaaatg
     4981 ctgcagcaaa taaatttgcc ggaacattaa gtgcattgat tcctccatcg ggcccggcag
     5041 ttgcaggtgc agtggttgag tatccatcat tccttggatt taaaatcact aacttatatg
     5101 aattctttat gctttttatt gtgatgacag gtgccgcggc tgctatatta tttgtgctta
     5161 gttcatggtt acaaaaaatg atgaacgaaa agcataatga ataccgcgaa gaactcgatc
     5221 gcttctaaca atttgaaaaa taaatgttcg gcagtgctgc cttagtttaa atcttcaatg
     5281 tttaaaatga atattaaatc cctcaactac tttgagggat tttttttatt ttaaattttc
     5341 gtatgcccta aaataatcaa tctcaaacga acacggaaaa acggtttggg catttggagg
     5401 gccatcataa aatcctccaa ctgcaaggtt caaaaatacc ggcatgggaa gctgtggcac
     5461 atgtgtggtt tttacatgca ccaattgacc gttaaaataa tattcgattt tgttcggtgc
     5521 caaatttaca ccgtaggtga aaaaatcatc agaaaggtcg agttttttat tgaaaaacca
     5581 ccatccattc aacttgggaa ttatcattgc attgtcgctc aaatcgcctg tagcagcctt
     5641 ccaaaaatta tttacccaat agcgcgaagt gttgctgccg ccaaattcga aaacatcaat
     5701 ctcctgatag taagttccgc tgcctttata aaaccaaaat gccggccaca aaccttttcc
     5761 cgatggcact tttgcgcgta cttcataata tccaaacgag aattctttct tcgaaataat
     5821 ttggcccgat gaataattga atctttcgtg tttttctttg tgtgcagtga gttttaaaat
     5881 tccgttctca ataaagatgt tttttttgct attgcgataa tattgttcct cgctgccatg
     5941 ctcaccggta tttactttcc aggcaattgt gtctaagttg tttagattaa actcttcgct
     6001 ccacaccatt ttagatttaa caattgtatt cccttttgaa tttggcagaa cagtttttac
     6061 gcgttgggca aaagtgcctt ttgataaaag cgtgaaacaa aatattacaa caattttttt
     6121 cataacggaa cgattttgga tatgcaaata aagttgcgaa taaagtaaaa caaaactgga
     6181 tatgtgaaat ttatatattg gttttggtat caacgaatta cgaatatgtt cgaatttacg
     6241 aattcaacaa attcgtaatt cggatttaat tcgaaattcg ttgacaagat tttgagttga
     6301 gtttgcaaaa tatctttaga tttgccgttc gaaaacaatt taaataataa atatatgaag
     6361 aagatttttt tgactttagc actttttggt gcatgtatca cattcgcttt ggcacaagaa
     6421 accaaggacg acaaaataaa taaaatgatt aaggatggtg ccgagtatgt gaaagcgaag
     6481 cagtacaaag aagctgcggg aagtttacaa gaggcaataa atatcattaa cgaaataatt
     6541 ggcgatgtaa tattgcaagg tttgccaaaa tcggcaggtg gccttacaag tgatcctgca
     6601 aatgaccaaa taacaagttt gggaacccta atgggagctg gcatgcaagt acaacgtatg
     6661 tattacaacg aaaaaggcca atcggtaaat atcactattc agccaaactc tccgcagtta
     6721 tcatctctca atacattctt agataatccc gatgattatc caggcgaaga agcaggaaag
     6781 cgtgtaaagg tgaaagattt caaatcttta ttacgtttta cgaaagccga aggtgaagaa
     6841 agttcctcat cgggctattt aacaatgcct ttttacactt ctgttgttac agtt
//
LOCUS       DAORQA010000081         6852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2861_length_6852_cov_10.281448, whole genome shotgun sequence.
ACCESSION   DAORQA010000081 DAORQA010000000
VERSION     DAORQA010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2861_length_6852_cov_10.281448"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2842
                     /locus_tag="PK736_05695"
     CDS             <1..2842
                     /locus_tag="PK736_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32917.1"
                     /translation="YSSPYMKAKPDSTGIYNFYDVQNAFQLYEKNNKANNELNKNKDE
                     SNEGEFEGYAQYKRWEWFSEQRVFPAGKFPAQEFISNEYQKFIDNYSTTTGRNNNLKT
                     ASNTWVNLSSPNMPVAAYSGMGRINCMAFMPGNSNTIFIGAACGGVWKSTNGGQTWTV
                     LNTDQLPSLSITSICIDPVNTNNIYLATGDNFTGIAGSLKPGHYSAGVFKSNDGGQTW
                     VLAGMPSLQSQMFIPQQMIIDPVTPSVMLMASNTGIWKTINSGTTWTLQQAGFFYSIE
                     FNPLNRNVVFATDWQGLWRSNNNGTNWVYRGGGYPNTFGAAARVTLAVTPADTNIVYT
                     WGKINNFSTRFKKYSNAGNTFNSFVNMTDPYAIAQPYGFVDLAIGVSKTNALNVMVGG
                     LTTAKSMDGGNTWVQASDYVNNLLPNYFHCDVKKIIFEPGSGSKLYALHDAGIAVSTD
                     NSATWNNISNGLQITEIYKIASDPSNSGIIYYGSQDNGTNRWNDSNDSIVMMTGGDGF
                     QPLVDPNNPMILFTSAQIGTFKKSIDGGANFISASPGVTLWNPPFKFNPLNSQTMYAG
                     CASGLKKSTMSGLQGSYVNMTASSLTAIKTFDVSKADTNYQYAASIDSIIGTTNNGAN
                     WNLITLGLPVNLAAISYVTCSSADPNKVWVTFSGYSAGNKVFMSNNGGGAWINYSGTL
                     PNIPVNCLLYVEGSNDEVIIGTDFGVFYRDASLSDWVSYNTGLPNVIVSHLDIHYGTM
                     KLRAGTFGRGLWEIGLPIATSLPVDLLYFTGKYNSENSTNDLQWTTASEKNCHYFDVM
                     KSADAKYFSAIALVNCNGYQSQIKNYYVTDNGNVSGTNYYYLKQVDFNEEVSNSNTIS
                     ITSGVDGQSILLYPNPAGNSIQFQFLRRHEIYYLQLFDAIGKEIILQPAIQLTSESNV
                     VEDISFLNSGTYFFKITNHINGQFFYGKFVKE"
     gene            complement(3478..4719)
                     /locus_tag="PK736_05700"
     CDS             complement(3478..4719)
                     /locus_tag="PK736_05700"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRC32918.1"
                     /translation="MKKIYLLLCITFVRITASAQWETVYFPTFGTQLGNLVAVNFSDF
                     NNGLAVGDSFPGTSPTPLILKTSDNGTTWNSVFGTAMAGTFTDVVYTTPFIAYAVGYH
                     YSSNTGFISKSLDGGNTWSTITFPALFIKVSFPSLNIGYVNASNGSIYKTTDGGISWN
                     PFSTPLSSLSQIEFINDTIGFASGIDTIIKTIDGGLSWSSYAFQQQGFLRIDFASDSI
                     GYALFSDFGKSYLYKTIDQGVNWYSLGIAATAPAYTTAINFPNDNVGFICGQFQMYKT
                     TDGGISWNAQTAKPPGWPNFFDDLMDLYFLNQDTGFAVGFNQYYRTSSGGLNFSENLN
                     SKSTISLYPNPGQDQITLTLNFRINNSHVTIYDCFGKKVYSSILVNNEEKIDCGLFTP
                     GIYFVTVNDGAKPYSKKLIIE"
     gene            complement(4770..6236)
                     /locus_tag="PK736_05705"
     CDS             complement(4770..6236)
                     /locus_tag="PK736_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HRC32919.1"
                     /translation="MSQAVRELEPKEMWNHFADLNAVPRPSKKEERIIEFMMQYGKKL
                     GLPTTKDEAGNVIIKKPASKGMENKIPIVMQSHLDMVHQKNGDTNFDFNTQGIEMFVD
                     GDWVKAKGTTLGADNGIGVASIMTVLESSTIAHPAFDALFTIDEETGMTGALNLKGGL
                     LDGKILLNLDTEDDNELTIGCAGGVDVTATGSYTFEFSPPNVGVFKISVTGLTGGHSG
                     MDIYKGRGNANKIMNRILLAAYEKFGIQISSIDGGSLRNAIPRESVAEIIIPSTEIDL
                     LAPFIQSIEKELKAECKTTDPNLSVALERIEGPKQVLQKGFQIKLLRCLYAAANGIYR
                     MSPDILHLVQTSNNLSRVLVKEGEYSIQCLTRSSVDSEKMDLANSLKSTFELIGARVE
                     FKGSYPGWTPKPDAAIVKIMSDLYTEMYKEDALVSACHAGLECGILGTNYPGMEMISF
                     GPNIRGAHSPDEKVQISSVQKYWAFFLETLKRIPEKSS"
     gene            complement(6340..>6852)
                     /locus_tag="PK736_05710"
     CDS             complement(6340..>6852)
                     /locus_tag="PK736_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HRC32920.1"
                     /translation="YQVCITGSNKSSDTQYWKNDFLNIKPASDNYHHTKDFLSVTREY
                     VTKQLSEEFEVSKADKIDLLNRSVAYFKENDKFEKKDFENEVIQDKKMIKSFRTFNET
                     YSAENDIEIADSFDISSQALKKQARIFKSVLKLDKNFHIYIHGDRELIEQGMDKDGRK
                     FYKIYFKEES"
BASE COUNT         2141 a         1248 c         1255 g         2208 t
ORIGIN      
        1 gtatagctca ccatacatga aagcaaaacc tgacagtaca ggcatttaca atttttatga
       61 tgtacaaaat gcttttcaat tgtatgaaaa aaataataaa gcgaataatg agttgaataa
      121 aaataaagac gaaagcaatg aaggggaatt tgaaggctat gcacaatata aacgttggga
      181 gtggtttagt gagcaacgtg tttttcctgc gggaaaattt cctgctcaag aatttatctc
      241 aaacgaatat caaaaattta ttgataatta ttccactact acggggcgca acaataattt
      301 aaaaaccgct tccaatacat gggtaaatct ctcatcgcca aatatgcctg ttgctgctta
      361 tagtggaatg ggccgaataa attgtatggc ttttatgccg ggaaatagta atacaatatt
      421 tataggtgct gcatgcggtg gagtttggaa aagtactaac ggtggccaaa catggacagt
      481 gttgaatacc gatcagttgc catcattaag tattacaagc atttgcattg accctgttaa
      541 tacgaacaac atttatcttg ctacgggcga taattttaca ggaattgccg gttcgttgaa
      601 acccgggcat tacagtgcag gtgttttcaa atcgaatgac ggtggacaaa catgggtgtt
      661 ggccggaatg ccatcattgc aatcgcaaat gtttattcca cagcaaatga ttatcgatcc
      721 tgttacacct tctgtgatgt tgatggcttc gaacaccggt atttggaaaa caattaatag
      781 cggaaccaca tggacacttc aacaagcagg ttttttttac agcatagaat ttaatcctct
      841 caatcgtaat gtggtttttg caaccgactg gcaagggttg tggcgttcaa ataataatgg
      901 cacaaattgg gtgtatcgcg gtggtggtta tcccaacact tttggtgcag ctgccagagt
      961 tacattagct gttacaccag ccgataccaa tatagtttat acatggggaa agataaataa
     1021 tttctctaca cgatttaaaa aatattcgaa tgctggtaat actttcaaca gttttgtaaa
     1081 tatgaccgat ccttatgcaa ttgcacagcc atatggattt gtagacctgg caatcggtgt
     1141 ttcgaaaact aatgcactaa atgtaatggt gggtggattg accacagcaa aaagtatgga
     1201 tggtggcaat acatgggtgc aggcatcgga ttatgtcaac aatcttttgc cgaattattt
     1261 tcattgtgat gtgaaaaaaa taattttcga acccggcagt ggcagcaaac tttatgcatt
     1321 gcacgatgcc ggtattgcag tgagtacaga taattctgcc acatggaaca acataagtaa
     1381 tggcttacaa attacagaga tttataaaat tgccagcgac ccatcgaact ccggcattat
     1441 atactatggt tcacaggaca atggtactaa ccgctggaat gattcgaacg acagcattgt
     1501 gatgatgaca ggaggcgatg gttttcagcc gcttgtagac cctaataacc caatgatact
     1561 ttttacaagt gcacaaatag gtactttcaa aaaatcaatt gatggagggg caaattttat
     1621 ttctgcatcg cctggtgtta cactttggaa tcctccattt aaatttaacc cattaaattc
     1681 tcaaacaatg tatgcgggtt gtgcatcggg attaaaaaag tcaacaatga gtggcttgca
     1741 gggaagttat gtgaacatga ctgcaagtag tcttacagcg attaaaacat ttgatgtttc
     1801 gaaagcagat acaaattacc aatatgcggc atcaattgat tctataatcg gcacaacaaa
     1861 caatggtgca aactggaact taattacact cggtttgccc gttaatttgg ctgctatttc
     1921 atatgtgact tgcagtagtg ccgacccgaa taaagtatgg gtaacttttt cagggtactc
     1981 ggccggcaat aaagttttta tgtcgaacaa tggaggaggt gcatggataa attactctgg
     2041 cactttacca aatattcctg ttaactgttt gctatatgtt gaaggcagca acgatgaagt
     2101 tattattgga accgactttg gcgtttttta tcgcgatgcc tctttgtcgg attgggtttc
     2161 ttacaatacc ggtttgccaa atgttattgt aagtcatctc gacattcatt atggcactat
     2221 gaaactgcgt gcaggcacct ttggccgcgg attgtgggag ataggtttgc ccatagctac
     2281 ttcgttacct gtcgatttat tgtattttac cggaaaatat aattctgaaa atagcaccaa
     2341 cgatttgcaa tggacaacag cctccgaaaa aaattgccac tattttgatg taatgaaaag
     2401 tgctgatgca aaatattttt cggctatagc acttgtaaat tgtaatggtt atcaatcgca
     2461 gataaaaaat tattatgtta ctgataatgg aaatgtaagt ggcacaaatt attattattt
     2521 gaaacaggta gattttaatg aggaggttag taattcaaat actatttcta ttacgagtgg
     2581 tgttgatgga caaagcattt tgctctatcc aaacccggca ggcaatagta ttcagtttca
     2641 atttttacga agacatgaga tttactacct gcaactattt gatgctatag gaaaagaaat
     2701 tattcttcaa cctgcaattc aactaacaag cgaatcgaat gtggtagaag atatttcgtt
     2761 tctaaatagt ggtacgtact tttttaaaat taccaatcac ataaacggac aattttttta
     2821 tggtaaattt gtaaaggagt gattgtaatt cgattttcat tctaagcccg tatggaacct
     2881 tttgtaaaat cggaaaaatg attttatatg ttttattgtc tcagtttaca cagctacacg
     2941 cgaaaagcac gatgggagac caaaaaaaaa tcaaatgctg attcttgttt ctttatgcgg
     3001 caaataacaa caacagcaat ggactcgaag aaacaccgta aaaatcgcaa agaaaaaaaa
     3061 agagaattaa aaaatgaaac gatggaaaca cagcacagca tttcttttgt atttctttgc
     3121 gataaaaaac atcaaccgca aaggtcacga agaaacaccg caaaattcgg aatgaagaaa
     3181 tatagataat taaaaaatgg aatgatgaaa acacagcgca gtattttctt tgaatctctc
     3241 acatgaggct tagtctctgt ctaagtgtct ttgtttaaca caatatccga tagtgtaata
     3301 taaattaatt tctccgttta gctttgtctc ctgcaatatc attttatgca tcaatcttca
     3361 aacgactaaa tcacacctat ttcaagtaca ttttcaaaac atggatttat ttaaaatttt
     3421 agcgtagtaa aagactgaga caaacggggg agatgattgt tttttgtaac aaaaacttta
     3481 ttcgataatc aattttttgg agtatggttt tgctccatcg tttacagtca caaaataaat
     3541 tcctggagta aataagccac aatcaatttt ttcttcgttg ttcacaagaa tgcttgagta
     3601 aacttttttt ccaaaacaat cgtaaatagt aacatgagaa ttatttattc gaaaatttaa
     3661 agtgagcgtt atttgatctt ggccaggatt agggtaaagt gaaattgttg attttgaatt
     3721 caaattttct gaaaaattta atcctccgga ggaggtgcga tagtattgat taaaacctac
     3781 agcgaaacct gtatcctgat ttaaaaaata aagatccatc aggtcatcaa agaaattggg
     3841 ccatcccggt ggttttgcag tttgagcatt ccatgatatg ccaccatctg ttgttttgta
     3901 catttgaaac tgcccacaaa taaatcctac attatcattc ggaaaattaa tagctgtggt
     3961 atatgcaggt gcggtggcag caatacctaa agaataccaa ttaactcctt ggtcaatagt
     4021 tttgtacaaa taacttttcc caaaatcaga aaataatgca taaccaattg aatcagaagc
     4081 aaaatcaatt ctaagaaatc cctgttgttg aaaagcataa ctgctccatg ataatccgcc
     4141 atcaattgtt ttgataatgg tgtcaattcc tgaagcgaaa ccaatagtat catttataaa
     4201 ctcaatttga ctaagactac tcaatggagt tgaaaatgga ttccatgaga ttccaccatc
     4261 agttgtttta taaatactac cattgcttgc atttacatat ccaatattta aactaggaaa
     4321 actaactttt ataaataaag caggaaatgt aatggttgac caggtgtttc cgccatctag
     4381 tgattttgat ataaatcctg tattactact ataatggtaa ccaactgcat atgcaataaa
     4441 aggtgtggta taaacaacat ctgtaaatgt gccagccatt gccgtgccaa atacagaatt
     4501 ccatgtagta ccattgtcac tggttttgag aattaaagga gtaggcgatg tcccgggaaa
     4561 cgagtcacca actgcaaggc cattattaaa atcggaaaaa tttacagcca caagatttcc
     4621 taattgagtg ccaaaagtag ggaaatagac agtttcccat tgagctgatg ctgtaattct
     4681 aacaaaagta atacaaagaa gcaagtaaat ctttttcatt ttgaattttt tataattttt
     4741 taatttctac gctaaaaaga atatcccctt taagagcttt tttccggtat tcgcttcaac
     4801 gtttctagaa aaaacgccca gtatttctgc accgaactta tttgtacttt ttcgtcagga
     4861 gaatgcgcac cgcgaatgtt aggaccaaaa gaaatcattt ccatgcctgg atagtttgta
     4921 cccaaaattc cacattctaa tcctgcatga catgcgctta ccaacgcatc ttctttgtac
     4981 atttcagtat acaggtcgct cattattttt acaattgctg catcgggttt tggagtccat
     5041 cccggatacg agcctttgaa ttctacacgt gcacctatca attcgaaagt ggattttaat
     5101 gaattggcaa ggtccatttt ttcgctatcg acagaactac gtgttaaaca ttgtatggaa
     5161 tattctcctt ctttcaccag cacacgcgac agattatttg atgtttgaac taaatgtaaa
     5221 atgtcaggac tcattctgta aatcccgttg gctgcggcat acaagcaacg caacaatttt
     5281 atttgaaacc ctttttgcaa aacttgtttt ggcccttcaa ttctttcgag tgcaacacta
     5341 agattgggat cggttgtttt gcattcggct tttagttctt tttctataga ttgaatgaaa
     5401 ggtgccagca aatctatttc ggttgaagga attattattt cggcaaccga ttcgcgaggt
     5461 atggcgttgc gcaaactacc accatcgatg ctactaattt gaatgccaaa tttttcgtat
     5521 gccgctaata aaatgcggtt cattatttta tttgcattgc cacgaccttt ataaatgtcc
     5581 atgccagagt gtccaccggt taatcctgtt actgatattt taaacacacc aacattgggt
     5641 ggcgaaaatt cgaaagtgta gctacccgtt gccgttacat caactccgcc tgcgcaacca
     5701 atggtaagtt cgttatcatc ttcggtatcg aggttcaata atattttgcc atcgagcaat
     5761 ccgcctttta aatttagggc tccggtcatg cctgtttctt cgtctatagt aaacaatgca
     5821 tcgaaggcag gatgagcaat agtgctcgat tccagtaccg tcattatact ggcaacgcca
     5881 ataccattat cggcaccaag ggttgtgcct tttgctttta cccaatcgcc atcaacaaac
     5941 atttctatac cttgtgtatt gaaatcgaaa ttggtatcac catttttttg atgcaccata
     6001 tcgagatgac tttgcatcac aattggaatt ttattttcca tgcctttgct cgcaggcttt
     6061 tttattatca cgttacctgc ttcatctttt gtagtgggca agccaagctt tttaccatac
     6121 tgcatcataa actctataat gcgttcttcc ttttttgaag ggcgtggtac tgcgttcaag
     6181 tccgcaaagt gattccacat ttcttttggt tctaattctc ttactgcttg tgacataatt
     6241 gtttttattt ttcctacaaa tgtatttaat tattgaaacg aactgttgta tagtcatttt
     6301 gaagtatctc tcgtttgatt attcaactcc atttaatgtt caactctcct ccttaaaata
     6361 aatcttataa aatttcctgc cgtctttatc cataccttgc tctataagtt ctctatcgcc
     6421 atgaatatat atatggaaat ttttatcgag ttttaaaaca cttttaaaaa tacgcgcttg
     6481 cttttttaat gcctgagaag aaatatcgaa actatcggca atttcaatat cgttttccgc
     6541 actgtaagtt tcgttaaatg tgcggaatga ttttatcatt tttttatcct gaataacttc
     6601 gttttcgaaa tctttttttt caaatttatc gttctcttta aaatatgcca ctgagcgatt
     6661 aagtaaatct atcttatcgg ctttcgatac ttcaaattct tcgctcagtt gtttggttac
     6721 atattcacgt gttaccgata aaaaatcttt tgtgtgatgg taattgtccg atgcaggttt
     6781 tatgttcagg aaatcatttt tccaatattg agtatcacta cttttgttgc tacctgtaat
     6841 gcatacttga ta
//
LOCUS       DAORQA010000082         6785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2892_length_6785_cov_9.511738, whole genome shotgun sequence.
ACCESSION   DAORQA010000082 DAORQA010000000
VERSION     DAORQA010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2892_length_6785_cov_9.511738"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..1506
                     /locus_tag="PK736_05715"
     CDS             316..1506
                     /locus_tag="PK736_05715"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRC32921.1"
                     /translation="MALAHRLSEISESQTIAMSKLSRELQQQGHDIVNLTLGEPDFVT
                     PDHIRMAAKKAIDEGYTHYTPIAGYADLRKAIVEKFKRENNLDFTTEQIVVSTGAKQS
                     IANVILCIINPGDEVIVPTPYWVSYSQIIILAGGIPVYVDAGIDTNFKITPAQLEATI
                     TPRTKCFIFSSPCNPSGTVYSRDELQALAKVFTNHPEVYVISDEIYEHIRTQPEHHSI
                     ASFSEIKNQVIVVNGVSKAYAMTGWRIGYIGAPLEIAKACDKIQGQFTSGTSSISQKA
                     AVAALNSTPQQMMPMLSAFAIRRKLMHENLSDIPHLKVNNPEGAFYFFPDVSYFFGKS
                     ADGKTIKSAEDLTMYLLADAKVSVVTGEAFGNGNCIRLSFATSDHLIVEACERIKNSL
                     SKLE"
     gene            1701..2576
                     /gene="sucD"
                     /locus_tag="PK736_05720"
     CDS             1701..2576
                     /gene="sucD"
                     /locus_tag="PK736_05720"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRC32922.1"
                     /translation="MSVLVNKQSKIIVQGFTGGEGTFHATQMIEFGTQVVGGVTPGKG
                     GTTHLDRPVFNTVREAVKATGANVSIVFVPPAFAADAIMEAADAGIALIVAITEGIPT
                     RDMIQVKEYLKDKNCRLIGPNCPGVITAGECKVGIMPGFVFKQGRVGIVSKSGTLTYE
                     AADQVAKAGLGVSTAIGIGGDPIIGTTTREAIELFMNDPDTDGIVMIGEIGGSLEAEA
                     ANWIKNNGNKKPVVGFIAGQTAPPGRRMGHAGAIIGGADDTAAAKMKIMRECGITVCD
                     SPADIGITMAKILAK"
     gene            2942..4573
                     /locus_tag="PK736_05725"
     CDS             2942..4573
                     /locus_tag="PK736_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32923.1"
                     /translation="MKTLSAIFTIFFCSYFNLSISQSFRTLTEGNLNYHCLVEDNDGS
                     FYLAAGWFMKFDNTGSFQFNKSYFEPKINQSCHFETWLNKTVAGDKIYMAIYYDSCFN
                     AIWGTPLLCKLANDGNVLKSKSFLLNGTCAALGNKFNILSSSNYLVHGYGGKPYNNLP
                     NASVVRDAPFALCVDSTLENVVYAKYFNRMGNSAFACEVENGSVVLGLNLDTIGPCLA
                     KLDSAGNILWCRSYMQGHGLFMDAVSNADGSIMVTGYTDTIAWGLGPFPSWFYTKLLL
                     MKIDSGGAVLWSKGYDDTHPYHFVIKRPAIIQKTYDGNYMLGATKITSNTMVSETAYL
                     LKVNTNGDLLWHNGFGLPSVAGPRTTELYNFIPTHDSCYLITGNFLGGNSWTWGGSFL
                     YKTDTSGTTPCGGNFVEPITTTPLFYSDSLITLGSIDGATVQDAYVIDTVGGPLNTMD
                     DCILLSTNNETTNTYHAPAFPNPTNGEVTVPSSKIFERDSYVTVYDSTGRIIIQYGIA
                     GKQTLKFNLSKFGSGLYLIKINEQGKIRTEQVVVE"
     gene            complement(4682..6256)
                     /locus_tag="PK736_05730"
     CDS             complement(4682..6256)
                     /locus_tag="PK736_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRC32924.1"
                     /translation="MPTIEIALPSAEGVSDTALEKELRKKLQILPGDKLQFRIIKKSL
                     DARSKNIKLVFLVEYVLNGELAPANFEKRKWQNVNNAKPVAIIGAGPAGLFAAIQLIE
                     LNYKPVIIERGKTVRDRRRDLAAINKQRIINPNSNYCFGEGGAGTYSDGKLYTRSTKR
                     GNVQRILYTLVECGANENILIETHPHIGTNKLPQIIETMRDKILACGGEFIFNTQFTG
                     FAKNGNTISGVQLSDVQSQHAFVLECSAVILATGHSARDVYQHFYDNRYHIECKPFAL
                     GVRIEHPQHIIDSVQYHCEVRPDNLPPAAYSLAYTDNNNRGTYSFCMCPGGIIAPAAT
                     APGELVVNGWSPSRRNNPFANSGMVVNIMEEDLKDFAHHGELRALYFQKHIEQQSFIA
                     GGENLTAPAQRLTDFLSDKHSATLPVCSYIPGITSVDLKNIFPTQITNALKEGLKVFG
                     KKMPSYITSEAVLVATESRTSSPVRIPRHAETLMHTNLKGLFPCAEGAGYAGGIVSAA
                     IDGERCADAVRNYFDA"
     gene            complement(6266..>6785)
                     /locus_tag="PK736_05735"
     CDS             complement(6266..>6785)
                     /locus_tag="PK736_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRC32925.1"
                     /translation="LYILDNIKQTGFNLSIVSDLHLGEYFDLRFLPGLSFAQRDLIYT
                     FYDNNVKGEETKKEIESTFLQLPVFVKFKSERVNNFRAYVLAGVEYNIDMVSQAKVRN
                     KDKDFVKLKRNDYGYSIGVGFDFYMEMFKFSPEVRMFNGIPNLKVADETIYARSLGAL
                     RSKIFTFSITFE"
BASE COUNT         2058 a         1323 c         1376 g         2028 t
ORIGIN      
        1 ccagaatgca cccctaaatc ccctttaggg gtcttgcccg ctacatactt ttgttttcat
       61 caattttcgt gattaaaata ttgtctctaa acgactttcc cttttttagt gatactttct
      121 atcaaaattc tatccttaca aggctttcac ggtttcttat taatagttta atagtagttt
      181 acatagctct atcaacaaat aaataaggtt aaccatcatt aaagtgccga ctaataattt
      241 tgtttaaaag aaagaaattt aaaaccatcg tgggtaaagt atttttatag ttttgtgccc
      301 ttatttttaa taaatatggc attagcacat cgactaagtg aaataagcga atcgcaaacc
      361 atagccatgt cgaaactgag ccgcgagctt cagcaacaag ggcatgatat tgtaaatctt
      421 acattgggtg agcccgattt tgttacaccc gatcatatac gcatggcagc aaaaaaagcc
      481 attgacgaag gctacactca ttatacccca atagctggct atgcagactt acgcaaagcg
      541 attgtggaaa aatttaaacg cgaaaacaac ctcgacttta ccaccgagca aattgtagta
      601 agcactggag caaaacaaag tatagccaac gttattttat gcattattaa tccgggcgat
      661 gaggtaatag tgcctacacc ctattgggta agttattcgc aaataattat tttggccggt
      721 ggcattccgg tttatgtgga tgccggtatc gataccaatt ttaagattac tcctgcacaa
      781 ctggaagcga ctattacgcc acgcacaaaa tgttttattt tttcttcgcc ttgcaatccc
      841 agtggcactg tttatagtcg cgatgagttg caggcattgg caaaggtttt taccaaccat
      901 cccgaagtgt atgtaataag tgatgaaatt tacgagcaca ttcgcaccca acccgaacat
      961 cacagcattg cttcttttag cgaaataaaa aatcaggtga tagttgtaaa cggtgtatcg
     1021 aaagcttatg ccatgacagg ttggcgaata ggatacatag gcgccccact cgaaattgcc
     1081 aaggcttgtg acaaaattca ggggcaattt acttcaggca catcatctat atcgcaaaag
     1141 gcggctgtgg cggcattaaa ttctacacca caacaaatga tgccaatgtt gagtgcattt
     1201 gctattcgca gaaaattgat gcacgaaaac ctcagcgata ttccccattt aaaggtgaac
     1261 aatcccgagg gagctttcta cttttttcct gatgtaagtt atttctttgg caaaagtgcc
     1321 gatggaaaaa ccataaaaag tgccgaagat ttgacaatgt atttacttgc cgatgccaaa
     1381 gtgagtgttg taacaggtga agcatttggt aacggtaact gtatacgctt atcatttgcc
     1441 accagtgatc atcttatagt agaagcttgc gaacggataa aaaacagtct ttcaaaatta
     1501 gaataaacat aaaattgtaa ggagacatca acgaattacg aatttgtacg attatacgaa
     1561 taaccgctat tcgtaaattc ggtattcatt cgtaattcgt tgattccaca tattactttt
     1621 tgctggctcc aaatattact ttgataacag ataaattttc agacttttgc acggcttttc
     1681 aaaaaaaata aatcaaacaa atgagtgtat tagttaacaa acaatcgaaa attatagttc
     1741 aggggtttac cggtggcgaa ggaacttttc atgccacaca aatgatagaa tttggcacac
     1801 aggtagtagg tggtgtaacg cccggcaaag gaggcactac gcacctcgac cgtcctgtat
     1861 ttaatactgt acgcgaagcg gtgaaagcaa caggtgctaa tgtgagtatt gtgtttgtac
     1921 cacccgcttt tgcagccgat gccattatgg aagcagccga tgccggcatt gcgcttatag
     1981 ttgctattac cgaaggtatt ccaacacgcg atatgataca ggtgaaagag tatttgaaag
     2041 ataaaaattg ccggctcata ggccctaact gccccggagt aattactgcc ggagaatgca
     2101 aagtaggcat catgccgggt tttgtattta agcaagggcg tgtaggtatt gtaagtaaaa
     2161 gcggcacatt gacatacgaa gcagccgatc aggttgccaa agccggcctt ggcgtaagca
     2221 cagcaatagg cataggaggc gacccaatta taggaaccac tacgcgcgaa gccatagaat
     2281 tatttatgaa tgaccctgat accgatggca ttgtgatgat aggtgaaata ggtggaagcc
     2341 tcgaagccga agcggccaac tggataaaaa ataacggcaa caaaaaacct gttgtaggat
     2401 ttattgccgg acaaaccgcc cctcccggaa ggcgcatggg ccatgccggt gccattattg
     2461 gtggtgccga tgatactgct gccgccaaaa tgaaaattat gcgcgagtgc ggtattaccg
     2521 tttgcgattc gcctgccgac attggcatta ctatggcgaa gattttagcc aagtaatttt
     2581 ttttaccaat aggatggatt gaaatccatc cttacaaaat ggagtcgagc ctacggctct
     2641 attgcttacg cgaaataatt aaatgcgcat taaaacccac aaggttttaa aaaccttgcg
     2701 ggttttttaa tacccctacc gaaaaagtga taataaaaaa catcacaatt gtaaccatgt
     2761 atattccgca ttttctgtat caagttatta agtatatttg ataagttttt taatcaagta
     2821 ataagtgtga ataattcaat caataaaaac attcaatgcg attttttagc taagggtgtt
     2881 tatcatatct gtgtttttaa ttcatcttca atttcatcta aacgcttaat aattaattga
     2941 aatgaaaaca ttaagtgcta tttttacaat atttttttgc tcctatttta atttaagtat
     3001 tagtcagtca tttagaactt tgactgaagg taatttaaac tatcattgtc ttgttgaaga
     3061 taatgatggt agtttttatt tggctgctgg ttggtttatg aaatttgaca acactggctc
     3121 atttcaattc aataaaagct attttgaacc aaaaataaat caaagttgcc attttgaaac
     3181 ttggttaaat aaaaccgttg cgggcgataa aatttatatg gctatttatt atgacagttg
     3241 ctttaatgct atctggggta cgcctctatt atgtaaattg gcgaacgatg gcaatgtgct
     3301 taaaagtaag tcgtttttac tcaacggcac atgtgctgca cttggaaata aatttaatat
     3361 tttaagttca agtaattatt tggttcatgg ttacggtggt aaaccataca acaaccttcc
     3421 aaatgcgagt gtagttcgtg atgccccctt cgccttatgt gtagattcca ctttagaaaa
     3481 tgttgtgtat gcaaaatatt ttaatcgtat gggcaatagt gcctttgctt gtgaggtaga
     3541 aaatggaagt gtggtacttg gtttaaattt agataccata ggcccatgcc tcgcaaaatt
     3601 agatagcgca ggtaacatat tatggtgccg cagttatatg caagggcatg gcctctttat
     3661 ggatgccgtt tcaaatgcag atggaagtat catggttacc ggatatacag atactattgc
     3721 ctggggtttg gggccatttc cctcttggtt ttatacaaaa ctattgctaa tgaaaattga
     3781 tagcggtggt gctgtgttat ggagcaaagg ttatgatgat acgcacccat atcattttgt
     3841 aataaagcgt ccagccatca ttcaaaaaac ttatgatggt aactacatgc ttggcgcaac
     3901 aaaaattact tcgaacacaa tggtatcgga aacagcctat ttgcttaaag taaatacaaa
     3961 tggtgatttg ttgtggcaca atggatttgg ccttccaagt gttgcagggc ctcgtacaac
     4021 agaattgtat aactttattc ccacacatga tagttgctat ttaataacag gcaattttct
     4081 tggcggcaat agttggactt ggggcggttc gtttttatac aaaaccgata caagcggcac
     4141 taccccatgc ggtggtaatt ttgttgagcc aattactacc acacctttat tttatagtga
     4201 tagcctcatt acgctcggca gtatagatgg tgcaacagtt caagatgctt atgtaattga
     4261 taccgtaggc gggccactca atacaatgga cgattgtatt ttgctatcaa ccaacaatga
     4321 aactacaaat acttaccacg cacctgcatt tcccaatcct acaaatggag aggttacagt
     4381 gccatcgagt aaaatatttg agcgcgatag ttatgtaacc gtgtacgata gcaccggccg
     4441 aataattata caatatggca ttgccggcaa gcaaacatta aagttcaatt tatcaaaatt
     4501 cggcagcggc ctgtacctga taaaaataaa cgagcaaggg aagattagaa cagagcaagt
     4561 ggttgtggag tgagttgttt tgtaggtata acagtggtta tatacgctga gacaaaacaa
     4621 aaatgaaagt agcaaaataa ccggaataaa cattgcccag ggttatcaaa aaaaatacgc
     4681 cctatgcatc aaaataattc ctcaccgcat cggcacatcg ctcgccatct attgctgcac
     4741 ttactattcc accggcatag cctgcccctt cggcacaggg gaacaagcct ttcaggtttg
     4801 tgtgcatcag tgtttcggca tggcgtggta tgcgaacggg cgatgatgta cgcgattctg
     4861 tggctaccaa tacggcttcg cttgtaatgt atgagggcat tttttttcca aatactttta
     4921 atccttcttt caatgcattt gtaatttgtg tggggaaaat atttttcaaa tccacacttg
     4981 ttatgccggg tatgtaactg caaaccggca aggttgcaga atgtttatcg ctcaaaaaat
     5041 cggtgaggcg ttgtgccggt gcagtaagat tttcgccacc ggcaataaaa ctttgttgtt
     5101 caatatgctt ttgaaaatac agcgcccgca attcaccatg atgtgcaaaa tccttcaaat
     5161 cttcttccat aatatttaca accatgcccg aattggcaaa aggattattt ctgcgcgagg
     5221 gcgaccaacc atttacaacc aactcgcccg gtgctgtggc tgcgggagcg ataattccgc
     5281 ccggacacat gcaaaaagaa taggtgccac gattattatt atcggtataa gctaatgaat
     5341 atgctgcagg cggcaaatta tcgggacgca cttcacagtg gtattgcaca ctatcaatta
     5401 tgtgttgtgg atgttcaata cgcacaccaa gtgcaaaggg tttacattct atatggtaac
     5461 gattgtcgta aaaatgttga tatacatcgc gtgccgagtg gcctgtggca agtataactg
     5521 cgctgcattc caatacaaat gcatgttgcg attgcacatc cgataattgc actccgctta
     5581 tggtattccc atttttcgca aaaccggtaa actgtgtatt gaaaataaat tcgcccccac
     5641 aagccagtat tttatcgcgc attgtttcta ttatctgtgg taatttattg gtgccaatat
     5701 gcggatgtgt ttcgatcaga atattttcgt ttgctccaca ctctaccaac gtatacagta
     5761 tacgttgtac attaccacgc ttggtggagc gggtatataa tttaccatcg gaatacgtac
     5821 cggcaccacc ttcgccaaag caataattac tgttgggatt aataatgcgc tgtttgttta
     5881 tagccgcaag gtcgcgcctg cgatcgcgta ctgttttgcc gcgttcaata attacaggct
     5941 tgtaatttag ttctatcaat tgaatagcgg caaataaacc tgcagggccg gcaccaatta
     6001 tggcaacagg ttttgcattg tttacatttt gccattttct tttttcgaaa ttggcaggag
     6061 ccaactcacc attcaaaaca tattcaacaa gaaaaaccaa cttgatattt ttggaacggg
     6121 catcgagtga tttttttatg atgcgaaatt gcagtttgtc acccggtaag atttgaagct
     6181 ttttacgaag ttctttttcg agtgctgtat cgctgacccc ttctgcagat ggcaatgcga
     6241 tttcgatggt tggcattttt attttttatt caaatgtgat ggagaaagta aaaatttttg
     6301 aacgcaatgc gccaagtgat cgggcataga tggtttcatc ggcaactttc aaattgggaa
     6361 taccattgaa catgcgcact tcgggcgaaa atttaaacat ctccatataa aaatcgaagc
     6421 ccacaccaat gctgtagcca tagtcgttgc gtttaagttt tacaaaatct ttatccttat
     6481 tgcgcacttt ggcctgcgat accatgtcaa tattatattc tacaccggct aacacgtatg
     6541 cacgaaaatt atttacccgc tcactcttga attttacaaa aaccggcaat tgcaaaaatg
     6601 tggattcaat ttcttttttg gtttcttctc cttttacatt gttgtcataa aaagtataaa
     6661 taagatcgcg ttgtgcaaac gataatccgg gcaaaaaacg caaatcgaaa tattcgccca
     6721 ggtgcaaatc gcttactata cttaaattaa atcctgtttg ttttatatta tcgagaatat
     6781 aaagc
//
LOCUS       DAORQA010000083         6706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2930_length_6706_cov_21.150053, whole genome shotgun sequence.
ACCESSION   DAORQA010000083 DAORQA010000000
VERSION     DAORQA010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2930_length_6706_cov_21.150053"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PK736_05740"
     CDS             <1..783
                     /locus_tag="PK736_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HRC32926.1"
                     /translation="DQKGKLIKTWGNEYPGAHGLTLFNENGEDVLFICDNNRHQVIKT
                     TIDGRVLLTLDYPKETGAYLNTTEYIPTETAIAPNGDIYVADGYGKDFIIQYDYKGNY
                     IRYWGGRGTEEKHLKNAHGICYDNRDKNNPVLIVTSREQNAFKRYTLDGIFIDTIDLP
                     GAWVCRPVIHSEYLYAAVLQSQSRLWQQSGFVTILDKNNKVVSNIAGNQPAYINNKLD
                     EMYQTIMAFKYPHDVCIDADENLYVAQWNSDHVYPYKLIPVA"
     gene            780..1571
                     /locus_tag="PK736_05745"
     CDS             780..1571
                     /locus_tag="PK736_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HRC32927.1"
                     /translation="MMKYYILLFLQTLTLSVLAQDVFQLAPPMLKYNSIFFTNETTVE
                     IKFAQSETTVHYTLNNSEPTLQDSIYTKPILIKNNFTTVKAKAFGKQFLPSYTVTVTF
                     IKDGFTVQSVESTLPNVKYPGNGANTLIDNNGGINQLGSNTWMGFNCDTVDIVMNLEK
                     EQTINKVLFDFLQSESSWIFIPEKIIVRWYDSASRSFKNFGTENFIAEKETSGSLCNF
                     KIITTTSKIKTNKISITLIPVKNMPAWHQAKGEHAWMFIDEIKLY"
     gene            1573..3723
                     /locus_tag="PK736_05750"
     CDS             1573..3723
                     /locus_tag="PK736_05750"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HRC32928.1"
                     /translation="MQRTKNILFNICFAINCLLVFLLLLDSRLEIPPAVQVIGRMHPL
                     LLHFPISLLILFVFYSLFSNKNNNANEQTKNIGDWLLLITAFTSSITALMGLLLSKEN
                     GYDADALQWHKWSGVSVAIITTLWFAYREKIKSINWLNTSTALLSLVIIVFTGHLGGE
                     ITHGENYLLSPILPETQQQTVLIEDATVYKDMVQPILKTKCMSCHNSKKAKGELVMET
                     EALLLKGGKNGKLWDSTAADFGLMLSRIHLPMEAKKHMPPTGKPQLTELEIQILNLWI
                     KSGADFNIKVASLNPSDSLRLIANSIFNTIETDDYDFDAADESKIKNLNSNYLLVEPI
                     SNGSPAVTASFFSAKFFSPEKLNALLTVKEQLISLNLNKTPTTDEQLKTIGQLIELRK
                     LNLAFTNITGKTLGELNKLQELRQLSLSGTSIKKDDVLKLSALKKLTHVYVWSTPLTD
                     ADIADLKSKMKNVEFEKGFKGDTTILKLTPPILLNEEQIINTPVNLNLKHYINGVTIH
                     YTLDGTEPDSIIAETYNNNIVLTHNVTVKAKAYKQGWYSSDVMEKYFFSSKYKPDSII
                     HLQPADVQYRGDGAKTLSDLVTGEATNFKNGKWVGFRENKMETLFLFDSIINVKSVTL
                     NSLVDVGSYIMPPVYIEVWGGTNKNNLKLLKRISPEQPTKLQSPFTKAFELSFTAANI
                     KSLKIISMPVPKLPAWHPGKGDKGWFFIDEVFIN"
     gene            complement(3759..6404)
                     /locus_tag="PK736_05755"
     CDS             complement(3759..6404)
                     /locus_tag="PK736_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRC32929.1"
                     /translation="MNKLLLYFIVFIFCYSAKGQSQVPYKNASLPVEERVRDLLSRMT
                     AEEKFRQLFMIAHDVEFDSVKYGNGIFGLELSAGKQNNAGAEQMIKNNSSLAADEMRN
                     NINAIQKFLVEQSRLGIPAIFFGEALHGVVAKGATTFPQSIALAATFDTALVHEVASA
                     IALETQERGLRQILSPVINLASDVRWGRVEETYGEDPFLSSEMAVAFVVPFEKKNIVT
                     TPKHFVANVGDGGRDSYPIHSDERTLNQNDFVPFKAAFIRGGARSVMSSYNSLNGEAC
                     SMNNYLLNKKLKQEWKFNGFVISDAGAVGGANVLHNTSKDYPTSGKLAIENGLDVIFQ
                     TSINHDTLFNKYFLDGSMDKAILDSAVVRVLRIKFELGLFEQSYALPVKVDYNKHVQL
                     AKQAAVESIVLLKNDNATLPLNKKIKSIAVIGTDAMECRLGGYSDEGNMKVNILDGIK
                     NEIGKTAEINFAEGVGRNQNLFACIDKKYLKTNDGESGIHAEYFGNINFSGAPLFQRN
                     ESQLNFHYTFYSPNEKISADFFSAKFTTKLTAPKTGIYKIGLEGNDGYRMYIDNKLVI
                     DRWSKISYHQQVIDFNFTAGKSYDIKIEFFEPAGNSQLKLIWNVESQDDADIKMAQAI
                     EVAKKSDVIIVVAGIDEGEFLDRDKLHLPGKQEEQISRLSSLGKPVVVLLVGGSAITM
                     DAWLDSVDAVMDIWYGGEQSGNAVAAILFGDQNPSGKLPITFPMCEGQLPLVYNHEPT
                     GRGDDYINSTGLPLFPFGYGLSYTTFEYSDMEIQKPVLTKNDSTTVTFTLKNTGKYDG
                     AEVVQLYMNQPVSKLTRPVLELKGFKKVFLKAGQTTQVTMPITREMFKQFNNQNEEIV
                     EAAKYKIMIGASSRDLRLKGVIVVR"
BASE COUNT         2213 a         1298 c         1131 g         2064 t
ORIGIN      
        1 gaccaaaaag gaaaactaat taaaacatgg ggcaatgaat atcctggtgc acatggccta
       61 actttattta atgaaaatgg cgaagacgtt ttatttattt gtgataacaa caggcaccag
      121 gtaattaaaa caaccattga tggccgcgta ttattaacgc tcgactatcc gaaagaaact
      181 ggcgcttatc ttaacactac agaatacatt ccaaccgaaa cagctattgc acctaatggt
      241 gacatatatg tagctgatgg ctacggaaaa gattttataa ttcagtatga ttataaaggc
      301 aattacatcc ggtattgggg cgggcgcgga acagaagaaa aacatttgaa aaatgctcat
      361 ggtatttgtt atgataaccg agataaaaac aatcctgttc tcatagttac ctcgcgagag
      421 caaaatgctt ttaaacgata tacacttgat ggaattttta ttgatacgat tgatttaccc
      481 ggtgcatggg tatgccgccc cgtaattcat agtgaatatt tatatgccgc agtattacaa
      541 agccagagca ggttgtggca gcaatcgggc tttgtaacga tattagataa aaacaataaa
      601 gttgtttcaa atattgccgg caaccaacct gcgtatatca acaataagtt agacgaaatg
      661 tatcaaacca tcatggcatt taaatatcca cacgatgtat gtattgatgc tgatgaaaac
      721 ttatatgtag cccaatggaa ttctgaccat gtatatcctt acaaactaat accggttgca
      781 tgatgaaata ttatatcctt ttatttcttc agacgttaac actgtcggtt ttagcacaag
      841 atgtttttca gttggcacct ccaatgttaa aatacaattc catttttttc acaaatgaaa
      901 ccactgtaga aataaaattt gcacaaagcg aaactacagt gcattacaca ttaaataatt
      961 cagaacccac gttgcaagac tccatttata ctaaacctat acttataaaa aataatttta
     1021 ctacagtaaa agcaaaagcc tttggcaaac agtttctccc gtcatataca gttaccgtta
     1081 cttttattaa agatggtttt acggtgcaat cggtggagtc aacactaccc aacgtaaaat
     1141 atcccggtaa tggtgccaat actttgattg ataataatgg tggaataaat cagcttggaa
     1201 gcaatacctg gatgggtttt aattgcgata cggttgatat agtaatgaac ttagaaaaag
     1261 aacaaactat aaataaagta ttgttcgatt ttttgcaaag tgaaagcagc tggattttta
     1321 tacccgaaaa aataatagtg cgttggtatg atagtgcatc gagaagtttt aaaaattttg
     1381 gaaccgaaaa ctttattgca gaaaaagaaa catcgggaag cctttgcaac tttaaaataa
     1441 tcaccacaac gagtaagata aaaacaaaca aaatttcgat aacccttata ccggtaaaaa
     1501 atatgccggc atggcatcag gcaaaaggcg agcatgcatg gatgtttatc gatgaaataa
     1561 aattgtatta atatgcagcg taccaaaaat attctattca atatttgttt tgccatcaat
     1621 tgcttgctgg tgtttttgtt gttgctggat agccggcttg aaataccgcc cgcagtgcaa
     1681 gtaataggac gcatgcaccc actgttactg cactttccaa tttcgttact tatacttttt
     1741 gttttttact ctttattcag caataaaaac aacaatgcaa atgagcagac aaaaaacatt
     1801 ggcgactggt tacttttaat tacagctttt acatcttcga ttacagcatt gatgggttta
     1861 ttgctgtcga aagaaaatgg atatgatgcc gatgctttgc aatggcataa atggagcggt
     1921 gtttcagttg caattattac aacgctatgg tttgcttatc gcgaaaaaat aaaaagcatc
     1981 aactggttaa atacttctac tgctttattg agtttagtaa ttatagtttt taccggacat
     2041 cttggaggtg aaattacaca tggagaaaat tatttactgt cgccaatatt gcccgaaaca
     2101 cagcagcaaa cggttttgat tgaagatgca acagtttata aagatatggt gcaacccatt
     2161 ttaaaaacca aatgcatgag ttgccataat agtaaaaaag caaaaggcga attggtgatg
     2221 gaaactgagg cactgctatt gaaaggtggg aagaatggaa aattatggga tagcacagca
     2281 gccgattttg gtttaatgct cagcaggata cacctcccaa tggaagccaa aaaacacatg
     2341 cctccaactg gtaagccaca gttgaccgag ctggaaattc aaattttaaa tttatggatt
     2401 aaaagcggtg ctgacttcaa tatcaaagtt gcatcactaa acccatctga ctcgcttcga
     2461 ttaattgcca attcgatttt caatactatt gaaaccgatg attatgattt tgacgcagca
     2521 gatgaatcaa aaattaaaaa tctaaattca aattatttgt tggtggagcc aatatcaaat
     2581 ggctcacctg ctgtgacagc atcgtttttc agtgctaagt ttttttcgcc cgaaaaatta
     2641 aacgcgctac ttacagttaa agaacaattg atatcactca atttaaataa aacacccaca
     2701 accgatgagc aattaaaaac cataggacaa cttattgaat tgcgaaaact caaccttgca
     2761 tttacaaaca tcaccggaaa aactttgggt gagttgaata aattacaaga actgcggcag
     2821 ttgtctttat caggaacatc aataaaaaaa gatgatgtgc ttaaattatc agcactgaaa
     2881 aaattaacac atgtttatgt ttggagcact ccactcaccg atgctgatat tgctgactta
     2941 aaaagtaaaa tgaaaaatgt tgaatttgaa aaaggattta aaggcgacac cacaatttta
     3001 aagctcaccc ctcctattct gcttaacgaa gagcaaataa ttaatactcc tgttaacctt
     3061 aatctaaaac attacatcaa tggcgtaaca atacattaca cattagatgg caccgaaccc
     3121 gacagtataa tcgctgaaac ttacaataac aatattgtgc tcacacataa tgtaaccgta
     3181 aaagcaaaag catataaaca aggttggtac agcagcgatg tgatggaaaa atattttttc
     3241 agttcaaagt ataaacccga ttcgataata catctgcaac ctgccgatgt gcaatataga
     3301 ggcgatggcg caaaaacatt atccgattta gtaaccggag aagctaccaa ttttaaaaac
     3361 ggtaaatggg ttggttttag agaaaataaa atggagacct tgtttttatt cgattcaata
     3421 ataaacgtaa aaagtgtaac gctaaattct ttagttgatg ttggcagcta tataatgcct
     3481 cctgtttaca ttgaagtatg gggtggtaca aacaaaaaca atttgaaatt attgaaacgc
     3541 atttcacctg aacaaccaac taaattacaa tccccattta ctaaagcatt tgaattatcg
     3601 tttactgcag ctaacatcaa atccttaaag attatttcta tgccggttcc aaaattacca
     3661 gcatggcatc ccggcaaagg cgataaagga tggtttttta ttgatgaggt ttttataaat
     3721 tgattatgtg aaataaatgt ataaacgcaa tctctatttt atctcaccac aatcaccccc
     3781 ttcaatctca aatctcttga cgaagcacca atcattattt tgtatttcgc agcttcaaca
     3841 atttcttcat tctgattatt aaattgctta aacatttcgc gggttattgg catggtaact
     3901 tgagtcgttt gccccgcctt taaaaacact tttttaaaac ctttaagttc gagcacaggc
     3961 cgcgtcaatt tgcttaccgg ttgattcata tataattgta ctacttcggc accatcgtat
     4021 ttgcctgtat ttttcaatgt gaatgtaact gtggtgctat cattttttgt tagcacgggt
     4081 ttttgaattt ccatatcgct atattcgaaa gttgtgtaac tcaatccata tccaaaagga
     4141 aataaaggca agcccgtaga gtttatataa tcatcgccac ggcctgtagg ttcatgattg
     4201 tagacaagag gcaattgacc ttcgcacatt ggaaatgtta ttggcagttt gccacttggg
     4261 ttttggtcgc caaataatat tgctgcaacg gcatttccac tttgctcacc gccataccat
     4321 atatccatta ctgcatctac actatcgagc catgcgtcca ttgtaatggc acttccacct
     4381 acaagcaata ccacaacagg ttttcctaag gaggataaac gacttatttg ctcctcttgc
     4441 ttgccgggta aatgcaactt atctctatca agaaattctc cttcatcaat tccagccact
     4501 actattatta catcgctttt ctttgcaact tcaattgcct gggccatctt tatatcagca
     4561 tcgtcttgcg attccacatt ccaaataagt tttaattgtg aattgccggc aggttcaaaa
     4621 aattctattt ttatatcata agattttccc gcagtaaaat tgaaatctat cacttgttgg
     4681 tgatacgaaa tttttgacca cctatcaatc accagtttat tgtcgatgta cattctgtat
     4741 ccgtcattac cttctaaacc tattttatat attccggttt taggagcggt tagctttgtg
     4801 gtaaattttg cagaaaaaaa gtcagcagaa attttttcgt tgggcgaata aaaagtgtaa
     4861 tgaaaattca attgactttc gttacgttga aatagtggtg cccccgaaaa attgatatta
     4921 ccaaaatact cggcatgtat acccgattcg ccatcatttg tttttaaata ttttttatcg
     4981 atgcatgcaa ataagttctg attgcggcca acaccttcgg caaaatttat ttctgcggtc
     5041 tttcctattt cattttttat accgtccaat atatttactt tcatatttcc ctcatcgcta
     5101 tagccaccca acctgcactc cattgcatca gtaccaatca ctgcgattga ttttattttc
     5161 ttattcaacg gcaatgtggc attatcattt ttcaacaaca caatgctttc aaccgctgcc
     5221 tgttttgcca attgcacatg tttgttgtaa tcaaccttta caggcaatgc atatgattgt
     5281 tcaaacaaac caagctcgaa ttttattctc aacacacgca ccacagcaga atcaagaatg
     5341 gccttatcca tgctgccatc aagaaagtat ttgttgaaca atgtatcatg gttgatagat
     5401 gtttgaaaaa taacatcaag gccgttttca attgctagct tacccgaagt aggataatct
     5461 ttcgaagtat tgtgcaacac atttgcgccc ccaacagcac cggcatcgga aattacaaaa
     5521 ccattaaatt tccattcttg ttttaatttt ttgttgagca aataattatt catactacac
     5581 gcctctccat tgagcgagtt gtaactactc attaccgacc ttgcgccacc tcgaataaac
     5641 gcagctttaa acggaacaaa atcattttgg tttaacgttc gttcatcgct atgaatggga
     5701 taactgtcgc gacctccatc accaacattg gctacaaaat gttttggtgt agtaacaata
     5761 tttttcttct caaaaggaac aacaaatgca acagccattt cggaacttag aaatggatct
     5821 tcgccataag tttcttctac ccttccccac ctaacatcgc ttgcgagatt tataaccgga
     5881 gaaagtattt gtcgcaaacc tctctcttgt gtttccaaag caatggcact tgccacttca
     5941 tgcacaagcg cagtatcgaa agtggcagcc aatgcaatag attgtggaaa agttgtagcg
     6001 ccttttgcaa caacaccatg aagcgcctca ccaaaaaata ttgccggaat tccaaggcgg
     6061 ctttgctcca caagaaattt ttgaatagca ttaatattat ttctcatttc atcggctgcc
     6121 aacgaagaat tattttttat catttgttcg gcacctgcgt tgttttgttt acccgcactg
     6181 agttccaatc cgaaaattcc attaccatac tttaccgaat caaattcaac atcatgtgca
     6241 atcataaata attgcctgaa tttttcttct gccgtcatac gacttaacaa atcgcgaaca
     6301 cgttcttcta ctggtagact agcatttttg taaggcactt gcgattggcc ttttgccgaa
     6361 taacaaaaaa taaaaactat aaagtatagc agtaatttat tcatagaaat aataattgaa
     6421 tttacgtacc ttaatttcca ctttaattaa tcacggaagg aaggcatcac aaagatggaa
     6481 taattttcat ttcacataca ttggtgtttt ataaaacctg ttactgaaaa attatttgat
     6541 tgggttattc attcacacga ttgtgcaaaa tattttggat agagacttac gccaaacgat
     6601 taagcattca gatatactaa gagtagttag attttcgaat gaaaatataa agtactctta
     6661 gtaatgccga acaacttcaa aagcttgttt ttccaaaacc taagtt
//
LOCUS       DAORQA010000084         6649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_2961_length_6649_cov_13.720352, whole genome shotgun sequence.
ACCESSION   DAORQA010000084 DAORQA010000000
VERSION     DAORQA010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2961_length_6649_cov_13.720352"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..460)
                     /locus_tag="PK736_05760"
     CDS             complement(44..460)
                     /locus_tag="PK736_05760"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRC32930.1"
                     /translation="MESSINSTNAPEPVGHYPHARRVGQLLFLSGVGPRERGTKIIPG
                     VTQDENGHVLSYDIEAQCHSVFKNVKAILEDSGSRWENIVDVTVYLTNMKNDFPVYNR
                     LWAEYFKVNPPCRTTLEINCLPTPIGIELKVIATID"
     gene            730..1086
                     /locus_tag="PK736_05765"
     CDS             730..1086
                     /locus_tag="PK736_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HRC32931.1"
                     /translation="MQKIYHLASCSTNQRILAEVKPGKKVVMQEIKTEPITAAQIDEM
                     KKLAGSYEALFSKIALKYRSMGLNQMQLTEKDYRKYILEEYTFLKRPVIINDNEIFIG
                     NAPKVVEAARISLQKK"
     gene            1119..2018
                     /locus_tag="PK736_05770"
     CDS             1119..2018
                     /locus_tag="PK736_05770"
                     /inference="COORDINATES: protein motif:HMM:NF017807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRC32932.1"
                     /translation="MKLKPNHNLLAHLAVLSANIIYGINYSVAKEVMPLYIQPFAFIF
                     LRVVSAAILFSLAHALFVREKVEKQDYIPLAMCGLFGVAINQLLFFKGLSLTQPINAA
                     LMMTTNPVQVMLLAYIFLKERITNVKIFGITLGLIGAASIIITGKQISMNGSTMLGDT
                     FIFLNSLSYAYFIIIAKPLLKKYHPVTVMKFAFLFGSIVVIPFGLNELSEVQWHVIPV
                     EAWYKITFIILATTFIAYLLNTFALRRLPSGTVSMYIYTQPVFATLMSIYLGLGHPQP
                     IHFIAATFIFVGVFLVSKPQAVK"
     gene            complement(2425..4071)
                     /locus_tag="PK736_05775"
     CDS             complement(2425..4071)
                     /locus_tag="PK736_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32933.1"
                     /translation="MKSNLLVLIIFVFVNVSSSQVPNWIWAKDAGSVSNDYGNAVCSD
                     ANGNVYACGTFQGSSITFSGYTVTNKGTGNLDFFVAKYDANGNVVWAKSGGGIFNDWV
                     YAICCDSKGNVYITGHFRSPLITFGTITLTNEDTVGNYEDIFIVKYDSLGNLIWAKKA
                     AGNSTDYSFSICCDANSNLYLTGMFESDTLNFGNASIVGLGNYDIFITKYDSVGNCLW
                     AMVAGNQFGAQGGLGICTDGIGNVYLTGFFQNYAVFSNDTVFGNGNADLFVAKYNTNG
                     NLSWVKSYNGIGWDWNSGSKLAADTFGNVYLTGYFAYSVIFGKDTLGNSGSFSIFTAK
                     LDSNGNALWGKSPGGTNNDFGNDITIDANINVYVTGYFTSSFLNFGGIPLMNNNVGYN
                     DAFVTEYDSSGNTIWATNVGGPDNDYGMGITISPGFDLYVIGYYGSYLMPFSNTTIVN
                     NGSNDVFLSKLGISTDLKENNVSDSYIYPNPFSDRLNILVNNNAQSEIKIYDIFSREI
                     MQIKFTSTYSINSMNLSNGIYIYEVLNNNEVINKGKLIKN"
     gene            complement(4673..5419)
                     /locus_tag="PK736_05780"
     CDS             complement(4673..5419)
                     /locus_tag="PK736_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32934.1"
                     /translation="MAKRSGDEIMQKIEEINLLLKSYDTLSPFSENEDRFELIDSIGN
                     RITAQLLVLLNDKRIVNYPIETLLFEEGIFISKSNDNKIFFFSIDEKTGGSYRTNITI
                     IHYRLSDGMVHAEFFGSEDYEAMATSIYDKVFLVDSAAQKYFVIGSVQTCNTCLASLA
                     IAITLDTSSYHVDLIAEFDGRQDDLKIFEFDSLEKIFSYEYFAASNDDSIYGGDNEMD
                     GLQHKYKNKYKYLNGAFFQIEKCEMWDNKE"
     gene            complement(5930..6172)
                     /locus_tag="PK736_05785"
     CDS             complement(5930..6172)
                     /locus_tag="PK736_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32935.1"
                     /translation="MGFKRDLNLVNNSVWWEPTDSLISSLQLGSKTYYNVYEQMLDTT
                     LAANQNKPVWKVYFTTADGLIGFNERFFNSSFVREY"
     assembly_gap    6250..6259
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6260..6635)
                     /locus_tag="PK736_05790"
     CDS             complement(<6260..6635)
                     /locus_tag="PK736_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32936.1"
                     /translation="MKTFILIFGITICAFCFYACSKKCDEEATIKCDGLSKKFADMVN
                     YEINDTIKFGNEKGNKKRFIVNYKKNTEAHNEYICHRDRYTCECEEVCDEQGAFVSEG
                     DSIYQIYNGYSVYVFEETFHGKG"
BASE COUNT         2168 a         1246 c         1025 g         2200 t
ORIGIN      
        1 atgtgtataa aaatgcctgt taaatattgc ttagcccgtt catttagtcg atggtggcta
       61 tcactttaag ttcaatacct atgggagtgg gcaaacaatt tatctctaaa gtggtgcggc
      121 aaggaggatt caccttaaag tactcggccc ataatcgatt gtaaacaggg aagtcgtttt
      181 tcatattggt gagataaacc gttacatcaa caatgttttc ccaccggctg ccgctgtctt
      241 ccaatattgc ttttacattt ttgaacaccg aatggcactg tgcctcaatg tcgtaagaaa
      301 gcacatgacc attttcgtcc tgtgttactc cgggaataat tttagtaccg cgttcacgcg
      361 gacccactcc cgacaaaaat aatagttgcc ccactcttcg tgcatgtgga taatgtccca
      421 ccggttcggg tgcattggta gaatttatac tcgactccat tgtttttaat tttttgttgg
      481 tggcaaaatt atctttttca atttagcatt tgactcatta ttgaaagtat ttccttctaa
      541 gcattaagaa aacaaatgct aaataagttt ttgctgcaaa tgggattaat aaataccgaa
      601 taatcctgtt gcatcttttt attcgaacta ttcaagttgc cattgaaact ttattgaaga
      661 tgaaatactt ttttttgcat atgtaaacct tgttatgctt tgtgttgtta tctatgcaat
      721 caaaaaaaaa tgcaaaaaat atatcatctg gcatcgtgct ctacaaatca gcgaatactt
      781 gccgaagtaa aacccggaaa aaaagtagtg atgcaggaaa taaaaaccga acccattact
      841 gccgcacaaa tcgatgagat gaaaaaactt gccggcagtt atgaggcact gtttagcaaa
      901 attgctttga agtaccgcag catgggcctc aaccaaatgc aactcaccga aaaggattac
      961 cgcaaatata ttcttgaaga gtatactttt ttgaaacgcc cggtgataat aaatgacaat
     1021 gaaatattta ttggcaacgc gcccaaagtg gtagaggctg caagaatttc tttacaaaaa
     1081 aaatgatatt gtaataattc ttattccttt gtggctttgt gaaactaaag cctaatcata
     1141 atcttcttgc acatttggcc gtactatctg ccaatataat ttatggaata aattattcgg
     1201 tggctaaaga agtaatgccg ctctacattc aaccatttgc ctttattttt ttacgtgttg
     1261 taagtgcagc tattttattc agcctggcac atgcattatt cgttcgcgaa aaagtagaaa
     1321 agcaagatta tattcccctg gccatgtgtg gattgtttgg cgtggctatc aatcagttat
     1381 tattctttaa aggcttatcg cttacacaac ctatcaatgc agcattgatg atgactacca
     1441 accccgttca ggtgatgtta ctggcatata tttttttgaa ggagcgcatt accaatgtca
     1501 aaatttttgg aattacgctt ggccttattg gtgcggcttc tatcattatc accggcaaac
     1561 aaatttcgat gaatggtagc accatgctgg gcgatacttt tatttttctt aactcccttt
     1621 cgtatgctta ctttataata attgccaaac ctcttttaaa aaaataccat cctgttacag
     1681 taatgaagtt tgctttttta ttcggcagca tcgtggttat tccttttggg ttaaatgaac
     1741 ttagtgaggt gcagtggcat gtaattccgg ttgaggcctg gtataaaatc accttcatca
     1801 ttcttgccac aacgtttatt gcctacttgc tcaatacttt tgctctgcga agattgcctt
     1861 cgggcacggt gagtatgtac atatacacac aaccggtttt tgccacactc atgagtattt
     1921 acttaggatt gggacatcct caaccaatac actttattgc agccacattt atttttgtgg
     1981 gagtctttct ggtgagtaaa ccacaggcag taaagtgatg taagagattt aaggcataat
     2041 ttgcaaacca tttttttatc cgagattgca atcagtttta acgaccatta agttttcgga
     2101 cggagcccaa aacacattgt cataccccaa acaataattc gattgataat aaaataaaac
     2161 tttgaaattg ttgtcctcgg ttggcgtccc ctccatccgt cttgcgcaaa tattagtatt
     2221 gcaataatag tcttaaactg aaattgctgt ggaaaatttc ctgtcaaatt aattgtgtgg
     2281 ttagtgtcaa aaattcaaaa tcattcataa aaaaacttca catttttgaa ttttaaaact
     2341 tttgtgaaat atacaaatgg tgggttgata gcttagggca aaaatcaatt tgttgtaaaa
     2401 tctaaaaaag gccattcttc tcgattaatt tttaatcaat ttacctttat taataacttc
     2461 attattattt agcacttcgt aaatataaat accattagaa agattcatgc tgtttattga
     2521 ataagtagat gtgaatttta tttgcatgat ttctcttgaa aaaatatcat aaattttaat
     2581 ttcggattgg gcgttattgt ttactaaaat atttaatcta tctgaaaagg ggtttggata
     2641 aatataactg tctgaaacat tgttttcctt taagtcagtg gatataccca atttagataa
     2701 aaaaacatca ttgctgccgt tatttacaat agtggtgttt gaaaatggca ttaaataact
     2761 accatagtat cctattacat acaagtcaaa acctggactt atggtaatac ccatgccata
     2821 atcattgtct ggcccgccta catttgtagc ccaaattgta ttgccgctgg aatcatattc
     2881 tgtaacaaac gcatcattat atcctacatt gttattcatt aagggaatgc caccaaagtt
     2941 gagaaatgaa ctcgtgaaat aacctgtcac ataaacattg atatttgcat caattgtaat
     3001 atcgttacca aaatcattgt ttgttcctcc agggcttttt ccccaaagtg cattgccatt
     3061 tgaatccaat tttgcagtaa aaatcgaaaa ggagccacta ttgcccaaag tatcctttcc
     3121 gaaaatcaca gaataagcaa aataaccagt taaatacaca ttgccaaatg tatctgctgc
     3181 taatttacta ccactattcc aatcccatcc tattccatta taacttttta cccaactcaa
     3241 attgccattt gtgttgtatt ttgcaacgaa taaatctgcg ttaccattac caaaaacagt
     3301 atcgttacta aagacagcgt aattttgaaa aaaacctgtt aaataaacat ttccaatgcc
     3361 atccgtgcaa atacctaagc caccttgcgc tccaaattga ttacctgcta ccattgccca
     3421 taaacagtta cctacagaat catactttgt tataaaaata tcataattac ctaatccgac
     3481 aatggaagca tttccaaaat tgagtgtatc actttcaaac attcctgtta aataaagatt
     3541 gctgttggca tcacaacaaa tactaaagct ataatcagtg ctatttccag ctgctttttt
     3601 tgcccaaatc aaattaccta aggagtcata tttgactata aaaatatcct catagttccc
     3661 tacagtatct tcattagtta aagtaattgt tccgaatgtt attaaaggag aacgaaaatg
     3721 acccgttata taaacattgc cttttgaatc gcaacatata gcatataccc aatcattaaa
     3781 tataccgcct ccgcttttag cccagactac attcccgttg gcatcatatt ttgctacaaa
     3841 aaagtcaagg ttgcctgtac ctttattagt tacagtatag ccagaaaaag taattgaaga
     3901 gccctgaaag gtcccacatg cataaacatt accgttagca tcagaacaaa cagcattacc
     3961 ataatcatta cttacactgc ctgcgtcttt agcccaaatc cagtttggca cttgagatga
     4021 gctaacattt acaaaaacaa aaatgattaa aaccaataaa ttagatttca tgacttacaa
     4081 tttttatcaa atacattttt atttagaaaa gatatataaa agatgattaa aaaattctag
     4141 ttaacaccaa actttgtaaa aataaaattt tattaatcac aacgcaatta tcattaagta
     4201 aaactaaaat ttaaaatacc acgtccgaca ttttttttta aaataaataa ttgaaacaaa
     4261 aattattagc tgaatcttct atccctgcca cccgccatgt tgcatacatt tgaattgcaa
     4321 caaaatcaca aacagaaatt tttttggttg aatacacatc aaatttatta catggtcatt
     4381 ggcaaaatct attgtagcaa aactttgcaa actttgcatt gaaatattta tgagaaaaaa
     4441 gaggttcgtg gtgagccaac caaggacaag aatcattcta aaattattga ttcgttattg
     4501 gtggccattt gaaaatatta agctcacaaa tatatgattt gtagctcaac aacctaaaaa
     4561 tttgagcttc aattggataa gttgtgagcc acaaattgaa tttactattt aaaaacttaa
     4621 cgagcacaaa acaaacattg acctttttta catgctgcac tgaacgttat tctcattctt
     4681 tattgtccca catttcacac ttttcaattt gaaaaaatgc tccgtttaaa tacttgtact
     4741 tgtttttgta tttatgttgc aacccatcca tttcgttgtc gccaccatat attgagtcgt
     4801 catttgaggc tgcaaaatat tcatacgaaa aaattttttc cagactgtcg aattcaaaaa
     4861 tttttaaatc gtcctgacgt ccatcaaact ctgcaattaa atcaacatga tatgaacttg
     4921 tatctaatgt aatagcaatt gccagcgaag caaggcatgt attacatgtt tgcacgcttc
     4981 caatcacaaa atatttttga gccgcactgt ccaccaaaaa cactttatcg taaatagaag
     5041 tggccatggc ttcgtaatct tcgctaccaa aaaactcggc atgcaccata ccatcggaaa
     5101 ggcgataatg tattattgta atgtttgttc gatatgagcc accggttttt tcatcaatcg
     5161 aaaagaaaaa tattttattg tcattcgatt ttgaaataaa aattccctct tcaaaaagaa
     5221 gcgtctcaat gggataatta actattcgtt tatcattcag taaaactaaa agttgtgcag
     5281 taattctatt gccgatggag tctattaatt caaatctatc ttcgttttcg gaaaatggcg
     5341 aaagggtgtc gtaagattta agcaacaaat ttatctcctc aattttttgc atgatttcgt
     5401 caccacttct tttagccaat ttgctctttt gctgacctaa tatattcgtt ggcaaaaaga
     5461 ataaaaaaaa caatggcaac attttcatcg ttgatttagc aaaaaaacat ttgaatattt
     5521 ttgtaagata aataatggtg gccatttgaa aatattaaga ttacaaatgt attatttgtt
     5581 gttcaacgga ctaaagtttt gagatacatt tggatttgga atgaggcact aagtgaaaat
     5641 aataattgta aatctgccct cggttggcgt ccccgccagc cgccttgctg catacattat
     5701 ttacatataa accaaaatca tatgctgcat ttgagacaat cgatttatta tcaaatcgcg
     5761 ttctagtgta ttgctaaaaa tccgaaataa atcatagcaa aatataaaaa ttgctgtgaa
     5821 atatttttcg aaataagcgg gtggcgggga cgccaacaaa ggacaaggaa ttcacattca
     5881 cctgttacat ctgttggtgg ctttgtgagg gcacatactt ttctaattgc taatactcac
     5941 gcacaaatga agaattaaaa aatctttcat taaatcctat gaggccatca gctgttgtaa
     6001 aataaacttt ccacactggc ttgttttgat tggcggccaa agtggtatct aacatttgct
     6061 cataaacatt ataatatgtt ttgcttccta attgcaaaga agaaatcaag gagtcagttg
     6121 gctcccacca gactgaatta tttactaaat tgaggtcacg tttaaaaccc aaaacatcta
     6181 aacggtaagt tgaatagttg cccttaccat gaaatctaat ttattggttt taatcatttt
     6241 tgtttttgtn nnnnnnnnnt gcccttacca tgaaacgttt cttcaaaaac atatactgaa
     6301 tagccgttat atatttggta tatactatcc ccctccgaca caaaagcacc ttgttcatca
     6361 caaacttcct cacactcgca agtgtatctg tctctatggc aaatatattc attatgtgcc
     6421 tcagtattct ttttataatt tacgataaat cgttttttat ttcccttttc atttccaaat
     6481 tttatggtat catttatttc gtagtttacc atgtcagcaa atttcttgct caggccgtca
     6541 cattttatgg tcgcttcttc gtcacatttt ttactgcatg cataaaaaca aaatgcacaa
     6601 atagtaattc caaaaattaa aataaatgtt ttcattttgg ctttgattt
//
LOCUS       DAORQA010000085         6572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3011_length_6572_cov_9.481203, whole genome shotgun sequence.
ACCESSION   DAORQA010000085 DAORQA010000000
VERSION     DAORQA010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3011_length_6572_cov_9.481203"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    347..356
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            868..1539
                     /locus_tag="PK736_05795"
     CDS             868..1539
                     /locus_tag="PK736_05795"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRC32937.1"
                     /translation="MITRISKYNFKNRLKKDLILKEKRKLQATKFFEKKAQNVAQIIL
                     SIPFICILLWSFQASNNKMIMSATGEIVISSHAPLETISAQSNQLKGVVNKADRTFAF
                     TVPIRSFEGFNSPLQKEHFNESYLESSKYANATFKGQIIEKINFDNDTVCFFRCKGLL
                     KIHGVEKERIIRLKMEVKQQQITVDGNFIVPLSDHNIYIPKIVMQKISSEIKTEISVR
                     FINTP"
     gene            1541..4273
                     /locus_tag="PK736_05800"
     CDS             1541..4273
                     /locus_tag="PK736_05800"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRC32938.1"
                     /translation="MRPRLFVLFVFISTAVLSQSPHFKSIPFDNFHINRIYTITADTN
                     GMIWLATDIGIMNYDGIKFRVAVENKIKQLSSLTITNQNIMIAGYQSGEAFFYKTSYS
                     KVVCIDSIRLKYPVKKALICDGITIVATYGGGCFFKQNNKWKPLNTETGLVDDYVNDM
                     VVFDNSLFIATDNGMSKIGCKNFPAIIKNYTPGDESNNANISNLALINNSNIYALTKQ
                     NGLYLYNPATDKLQHDSITQNWTYGEVKCAYADNDELWIGTSGNVVVDIDWREKTSLR
                     LFDYNSGFTYTDLQAIYRDRNSGFWVATNNNLLYSIGERMEILRNIGDVNIGEVNAIE
                     IDANGGRYFANKKGVFCLPDNTAGSKLEKINLPSPYQNESVMCMYLDAKNYLWMGTFN
                     NVLLRYNTVKKTFETFTEKNGLTNASAICITGNESTIWLATLGGLYTCDINNKQINFV
                     HQPKQSELAGTYIYKILLEKNGTIWYATDGNGLIKENNGNYIRYGEDILPQLKIIYSM
                     AKDNMGNLWCGTAKGGLLKFDCKNFTPVNYPLLNNHSVYTVTTDRYNHIIVTTDDAII
                     TIDARDNTIIAFTGEQGLFELKPNPNVVAKTRHDEMYFGVYSGIVNINPRFFKKEKTP
                     QLVMHNILLFSQPVNAEKAIFDYNKNYIGFEFAALNYDDPKSILYQYRLKGYNKEWRE
                     TGDNNVSFPNLPPGNYEFQLRASSQTIFNNKNKLSVRFKIKQPFYATSFFIIGAITTL
                     LVLAFFVVRLRIKNIRKMEALKKDKLLFELEGLKMQVNPHFLHNSFNTLINIIETDTY
                     AAVTYVEKLSDFFRNILMLQTKDLVTLKEELLLARDYFYLQQQRFGNAISLSINIGPQ
                     AESKMIPPLTLQMLLENIFKHNTISNRSKITIEISEKNGDCIGNKKHAYCQTKY"
     gene            4221..4391
                     /locus_tag="PK736_05805"
     CDS             4221..4391
                     /locus_tag="PK736_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32939.1"
                     /translation="MVIALEIKNTRIAKLNIENSTGIGLKNIESRYNIFTQRKLEIIA
                     TDTEFKVIVPLL"
     gene            4403..5173
                     /locus_tag="PK736_05810"
     CDS             4403..5173
                     /locus_tag="PK736_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRC32940.1"
                     /translation="MKKTVLIVEDEMPAASRLEKMVTQANDEYNIVAKLESIADTVLW
                     LKQNEAPDLILMDIQIADGLCFDIFKKADVTSPVIFTTAYDEYALEAFKVNSIDYLLK
                     PIKKEELHRALQKFEKLNFKVPAADYINIGEKVAKKQHSKLLRIIVRYADVIKAIDLN
                     DVAYFYTENKINFVCTKTNERYGIDYNLEEMEHLVDEVKFFRINRQFIININAIAKMF
                     NYSKSRIKMVLNPPSEIETIVSTERAADFKKWLTGTAE"
     gene            5329..6162
                     /locus_tag="PK736_05815"
     CDS             5329..6162
                     /locus_tag="PK736_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32941.1"
                     /translation="MKFKTNKIIALIALMGLGVVLTSGTEGTLSDDGKAGSTGSPGET
                     TCVTCHNSYALNSGGGSVSLACNMPSWQYTPGTTYSMSVTVARAGNGLFGFGIEALTA
                     AGQNAGTFVITNAAKTQIKSATVNNVSRKNVVHQKNAGIGTGSYTFDFDWTAPMTNVG
                     TVSFYFAGNAANGNGGTTNDYVYSGLQTATPSSTSIGEVSSNSNIEMLYSGINNTLNI
                     TGLKEASTVTIMDINGNIQLIRKVKIDEAIELPQLTNGTYIVTVTGEQQSTRQKFMIM
                     N"
BASE COUNT         2288 a         1175 c         1249 g         1850 t
ORIGIN      
        1 ttgttaataa tgagctaatt caaaacttga agtgttgtta aacaaaatca aatttgtggg
       61 gagaaagcac cactatttat tgaggtgcaa tgttattaaa agaaacatta tttgcaaatt
      121 gaacatagca gatagttatt aacaatagat tatgtaagcc gttaatatat gtcaacctat
      181 tgactttgtg tgaattagca actaactttt gatttggaga gggcaaaaat ttaatataga
      241 aatgccaaaa tacgctgtgg caaacggagt aaaaagtagt ttaatttaat agctgagtct
      301 actccgtaaa attggggaaa ataaaatgcc gccaaaaaac caactcnnnn nnnnnnatgt
      361 gaaatgatat gttcttatgg ctaaaataat tattggaagt ggactcaaag tccaagaatt
      421 taataagtgc tttttggaat gacaactatt gggagaatac cagttcaaat aaaaattgta
      481 tgtcagctat ttaacaaaca acaaataatt atttgaagtt atgacgaaaa aatagttctt
      541 acaaaccgat ataaaattat tcatcacgcc aaaatattat tgaatgatga taaattacga
      601 ttataatttt tgtaaaaaat gcagtataaa ttgtaaagta aaataaaacc gaataatgga
      661 aaatgataaa cattatgccc ggcagaaaaa ctgtaatcag gtaaataaaa ttacagtact
      721 ttgcttgttc aatagcataa aataccggtt gaaagttttt aatttgacaa tcagcacaaa
      781 agttgtgctt atttactgaa gtacaaaaac aaaaagtcca aaactgattt gctaagcttg
      841 gatagagtac aacaaataaa acaatatttg ataacacgta tttcgaaata caatttcaaa
      901 aatagattga aaaaggattt gatacttaaa gaaaaaagaa aattgcaggc gacaaaattt
      961 ttcgaaaaaa aggcacaaaa tgttgcgcaa attattttgt caataccttt tatttgcatt
     1021 ttgctgtggt cgtttcaggc cagtaataat aaaatgataa tgtcggccac gggcgaaatt
     1081 gtgataagca gccacgcccc tctcgaaaca ataagcgcac aaagtaatca gttgaaaggt
     1141 gtggttaata aggccgaccg cacctttgcg tttactgttc ccattcgctc gttcgaaggt
     1201 tttaatagtc ccttgcaaaa ggaacacttc aacgaatcgt atcttgaaag cagtaaatat
     1261 gcaaacgcca cttttaaagg tcagataatt gaaaaaataa atttcgataa cgatactgtt
     1321 tgcttcttca ggtgcaaagg attattgaaa atacacggtg tagaaaagga aagaataatc
     1381 aggctgaaaa tggaagtgaa gcaacagcaa ataacagttg atggtaattt tattgtgccc
     1441 ttatcggacc ataatattta tattccgaaa atagtaatgc aaaaaatatc aagcgaaata
     1501 aaaacagaaa tatcagtacg gtttatcaat actccataac atgcgaccac ggctttttgt
     1561 cttattcgtt tttatctcta cggcagtgtt gtcgcagtcg ccacatttta agtcgatacc
     1621 gttcgataat tttcacatca acagaatata taccatcact gccgatacaa atggcatgat
     1681 atggcttgct accgatattg gaataatgaa ttacgatggg ataaaatttc gggtggcggt
     1741 agaaaataaa ataaaacaac tcagcagcct taccattacc aaccaaaaca taatgatagc
     1801 ggggtaccag tcaggcgaag cattttttta taaaaccagc tattcgaaag ttgtttgcat
     1861 tgattcgata cgactaaaat atcctgtaaa aaaagctttg atatgcgatg gtataaccat
     1921 agtggccacc tatggcggag gatgcttttt caaacaaaat aataaatgga aaccgctgaa
     1981 taccgaaacc ggactggtag atgattatgt aaacgatatg gtggtttttg acaacagttt
     2041 atttattgcc accgataatg gtatgagtaa aataggctgc aaaaattttc ccgccatcat
     2101 aaaaaattat acccccggtg atgaatcgaa caatgccaat atttcgaacc tggcgctgat
     2161 aaacaattcg aatatttatg cacttaccaa acaaaatggc ttataccttt ataacccggc
     2221 taccgacaaa ctacaacacg atagcataac ccaaaactgg acgtatggtg aagtgaaatg
     2281 cgcctatgcc gataacgatg aactttggat tggcacttcg ggcaatgtag tggttgatat
     2341 tgactggcgc gaaaaaacaa gcttacgtct tttcgattac aattcgggat ttacctatac
     2401 cgatttgcag gcaatatacc gcgaccgtaa cagtggcttt tgggtggcaa caaataataa
     2461 cctgttgtac agcattggcg aaagaatgga aatattgcgc aacataggcg atgttaatat
     2521 tggtgaagta aatgccattg aaatagatgc aaacggtggt cgatatttcg ccaataagaa
     2581 gggtgtattc tgccttcccg ataatactgc aggcagcaaa ttagaaaaga taaacctgcc
     2641 ctcgccttac cagaatgaaa gtgtgatgtg tatgtatctt gatgccaaaa attatttatg
     2701 gatgggcact ttcaacaatg tgttgctgcg ctacaatacc gttaagaaaa cgtttgaaac
     2761 ctttaccgaa aaaaacggat taaccaatgc cagtgccatt tgcatcaccg gcaacgaaag
     2821 caccatatgg cttgcaacac tcggtggttt atatacctgc gatatcaaca ataagcaaat
     2881 caattttgtg catcaaccca aacaaagcga acttgccggc acttatattt ataaaatact
     2941 gctcgaaaaa aacggcacca tttggtatgc aaccgatggc aatgggttga taaaagaaaa
     3001 taacggtaac tatatacgtt acggagaaga tattttacca cagctgaaga taatatattc
     3061 tatggccaaa gacaatatgg gtaatttgtg gtgtggtact gccaaaggag ggttgctaaa
     3121 atttgattgc aaaaatttta ctccggtaaa ctatcctttg ctcaataacc attcggtgta
     3181 cactgtaact accgacaggt acaatcacat tattgtaact acagacgatg ccataataac
     3241 catagatgca cgtgacaata ccattattgc atttacaggt gaacagggat tgtttgaact
     3301 taagcccaac ccaaatgtgg ttgcaaaaac acggcacgat gaaatgtatt ttggcgttta
     3361 cagcggcata gtgaacatta atccgcgctt ttttaaaaag gagaaaacac cccaattggt
     3421 gatgcacaat atattattgt tttcgcagcc tgttaatgcc gaaaaagcaa tattcgatta
     3481 caataaaaat tatataggtt tcgaatttgc agcattgaac tatgatgatc ccaaatctat
     3541 attgtatcag taccggctta aaggatacaa caaagaatgg cgcgaaacgg gcgataacaa
     3601 tgtgtcgttc cccaatttgc caccgggaaa ttacgaattt caattgcggg catcatcaca
     3661 aacaatattc aataataaaa ataaactgtc ggtacgtttt aaaataaagc agccttttta
     3721 tgccacatct ttttttatta ttggcgcgat tactacactg ttggttttag cattttttgt
     3781 tgtcaggttg aggataaaaa acatccgaaa aatggaggct ttgaaaaagg ataaactatt
     3841 attcgaactc gaaggattga aaatgcaggt gaaccctcac ttccttcaca acagttttaa
     3901 tacgcttatc aatattattg aaacagatac atatgcggca gttacttatg tagaaaagtt
     3961 atcggatttt tttcgcaata ttctcatgct gcaaaccaaa gatttggtaa cacttaaaga
     4021 agaactttta ctggctcgtg attattttta tttgcaacaa cagcggtttg gaaatgccat
     4081 ttcattatcc ataaatattg ggccgcaggc agaatcaaaa atgattcctc cgctgacatt
     4141 acaaatgctg ctcgaaaata tttttaagca taatactatc tcgaaccgat caaaaattac
     4201 catcgaaata agtgaaaaaa atggtgattg cattggaaat aaaaaacacg cgtattgcca
     4261 aactaaatat tgagaattcg accggtatag gattaaagaa tatagaatcg cgctataata
     4321 tttttacaca acgcaaactg gaaattattg ccaccgatac tgaatttaaa gtaatagtac
     4381 ctttattata aaaaagaaaa atatgaaaaa aacagtactg atagttgagg atgaaatgcc
     4441 cgcagcatcg cggttagaaa aaatggttac gcaagccaat gacgaatata atattgtggc
     4501 aaaactggag agcattgccg acacagtgtt gtggctcaaa caaaacgaag cccccgattt
     4561 gatattgatg gatattcaaa ttgccgatgg attgtgtttc gatattttta aaaaagccga
     4621 tgttaccagc ccggttattt ttacaacagc atatgacgag tatgcactcg aagcatttaa
     4681 agtgaacagc attgattatt tgttgaagcc aataaaaaaa gaagaactac accgggcatt
     4741 gcaaaaattc gaaaagctga atttcaaagt acccgctgcc gattacataa acattggaga
     4801 aaaagttgca aaaaaacaac atagcaagtt acttcgaata atagtaagat atgccgatgt
     4861 gataaaggct atagacctga atgatgtagc ctatttttac accgaaaata aaatcaactt
     4921 tgtgtgtaca aaaacaaacg aaaggtatgg catagattat aacctcgaag aaatggaaca
     4981 tcttgttgat gaggttaaat ttttccgcat caaccgccag ttcattatca atataaatgc
     5041 aattgcaaaa atgtttaact actctaaatc gcgaataaaa atggtgctca atccaccaag
     5101 cgaaattgaa accatagtaa gcaccgaacg tgcggcagat ttcaagaaat ggctcacagg
     5161 cactgccgaa taattagcgt atatgcccat ttgaggaatc tttttttccg gcttagcgaa
     5221 tctttttgaa actttgagtg ttttaacatt tggatgcatt actttcgcaa tcgcaaaacc
     5281 acataagggt ttttcaactg aagaaaattt atttatttaa tttgtaatat gaaatttaaa
     5341 acaaacaaaa ttatcgcgct tattgcgcta atgggtttag gcgtagtgct aacatcgggc
     5401 acagaaggta cgcttagtga cgatggaaaa gcaggctcta cgggctcacc cggagagact
     5461 acatgcgtca cttgccacaa ttcatatgca ctcaatagcg gtggtggttc ggtaagctta
     5521 gcttgtaata tgccttcgtg gcaatacaca ccgggtacta catacagcat gagtgtaact
     5581 gtggcacgcg caggaaatgg tttatttggt tttggaattg aagcacttac tgcggcaggc
     5641 caaaatgcag gaacatttgt aattactaat gcggctaaaa cgcaaataaa atcggccacg
     5701 gtaaataatg tgagcaggaa aaatgtggtg catcaaaaaa atgccggcat aggaaccggt
     5761 tcatatactt tcgatttcga ctggactgca ccaatgacaa atgtgggcac agtaagtttt
     5821 tattttgcag gaaatgctgc taatggtaac ggtggtacca ccaacgatta tgtttattct
     5881 ggattgcaaa ctgcaacgcc ttcgtccaca tcaattggcg aagtttcaag caattcaaat
     5941 attgaaatgt tatattctgg catcaataac acactaaaca ttacgggatt aaaagaagct
     6001 tcaacagtta ctattatgga catcaatggt aatatacagc ttatacgcaa agtaaagata
     6061 gatgaggcca tagaattacc acaacttact aacggcacgt atatagttac cgttacaggc
     6121 gagcaacaat caactcgtca aaagtttatg ataatgaatt agggatttag ggttttataa
     6181 aggttgtact tttcggccgg caaggtttat ctgcaaaggt aaatgttgcc ggtttttttg
     6241 tggttatgtt ttcatccggg tcaagggtgg agcagcattt ttcctgtcct cggtaggcgt
     6301 ccccgccaac cgcatgcgtt gatttttatg cattcatcca tcactaactt ttgaatgcct
     6361 gtgtattcct gtcttcggtt ggcgtccccg ccaaccgcat ccattgagac ttatacgttc
     6421 atccatcact aactattgaa tgcctttcca ttcctgtcct cggttggcgt ccccgccaac
     6481 cgcatccatt gagatttatg cattcattca tcactaacta ttgaatgcct gtgcattcct
     6541 gtcctcggtt ggcgtccccg ccaaacgcat cc
//
LOCUS       DAORQA010000086         6523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3035_length_6523_cov_7.001855, whole genome shotgun sequence.
ACCESSION   DAORQA010000086 DAORQA010000000
VERSION     DAORQA010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3035_length_6523_cov_7.001855"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..2664)
                     /locus_tag="PK736_05820"
     CDS             complement(1..2664)
                     /locus_tag="PK736_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32942.1"
                     /translation="MKKIILIIMWCCMYMHMSAQERNFRRDLSDIKVGDSFDSICKVY
                     DAWYETNGKQRGSGYVQYSRWKHDTQMRLDGNKRIINSTAKTWESYYAHELAIKNNAS
                     QKAMMAPVANWQSMGPTNFTLGLTGYAGGIGRVNTVAFQGNSFGASEFWVGTPAGGIW
                     KTNTGGNSWNSISNGLPVLGVSGICVHPTISNTIYILTGDGDGGNTKSVGVLKSINGG
                     LTWNTTGLSFTVVQQVNGYKLMMDPTNSNVLYAVTSFGVYKTDNAASTWTLVLAGNAR
                     DAEFKPGNSSTVYCAIDTVVKYSTNSGSTWNTAAGLPGSNIARIAIAVSPASPNRVYA
                     LLGQAGKIGGFRGFYVSNNSAANFSFKSNSPAILCAQDDGSSGDSAAQSGYDLALAVH
                     NTDPSIVIVGGINVWRSVDTGATWTLSSHWVQNANDTNYVHADIHDLKFTDGDNVLYA
                     CSDGGIFLSTNNGSTWEDISTGLNISQYYSIDVSQGNNNYVMAGAQDNGCNYIDVGAN
                     TNLHVKGADGMQVAIQPSNKDVIYYSWQGGGIFRSDSGGTSALESYISPPNSGDGLFF
                     TPFILDKQNENRLYAGYRKIFRSNDKGDTWNAITDSLPYLVSKLALSTINNQQMIRVI
                     QYSWIDRTANALAALPTFTFVNDTDIAFPITNVVFSERNATQALLTMGGYNPNKKVVY
                     SNDGGVTWIPLNTGLPNVPINCAAFHPSYNNYLFVGTDVGVYMYDLNTSGPWTAYNNG
                     LPNVIVTDLKIYAATNTLVAGTYSRGLWRTPLPQLCLGNLFLDNTSPSSAAEYWHGAG
                     TVTSTDNMTWGNGAAVTYKAQTYIQLNPGFFVGNSMTFNANIEGCAINNLLSKQTGTL
                     AGPMVSEYEASRTNKDLIHCFKFSTFLTFCI"
     gene            complement(2887..4470)
                     /locus_tag="PK736_05825"
     CDS             complement(2887..4470)
                     /locus_tag="PK736_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32943.1"
                     /translation="MLNKVKSRNSGRKTKMILAIAACVVALQAQAQWTKTGAVIAPTV
                     LTDNVSVGTTVNGANIYAEKTAAVNLGIKSSTLSATMFMDKGSAAANAAFAYKTLGTT
                     LWNSGMLGNNNFNIKNVSLNTTPLRIDLANDNMALCTQGGKVGIGNASPSFKLDISGD
                     CRINTAPQNTTFNSLILNSNTTATSYDKYEPLKLINTGNFGGATNSIGFYGGFGSNIG
                     KITSILNSSGNGAGMLFSVGDNNLLIQPRMMIDNQGSVGIGTTSPSAKLTVQTQSEVY
                     GIVHTDGTVKMGTYIGLNVYNNLAGGWLGTESDHPMYFMTNNNWPQITLLQNGNVGFG
                     SSNPHAQLQFDNGLNNRRIVLWEGVDNEHQFAGFGMNANTLRYQVEHLGGNHVFYAAV
                     DSTSSNELMRIQGDGRVAIGGDASSNYKLTVRGSIICTDLRVQLQPFPDYVFANDYKL
                     LPLNELENYVNKNNHLPGMPTATEVEEQEVTVGQLQMKLVEKVEELTLYVIELNKANE
                     QLKNNNAALVERVKLLEKK"
     gene            complement(4904..>6190)
                     /locus_tag="PK736_05830"
     CDS             complement(4904..>6190)
                     /locus_tag="PK736_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HRC32944.1"
                     /translation="FICVTIDFDSFNAKGGGGNQTFTTKYEGNCAKIFFDCSFSYYQN
                     KGSNTTNVTNYTNSLFNVISTIYYNDSISLGISAIHIWTVVDPFDHSSGGNALNTFRT
                     YYTTNTYPGSIAAFLSTSIGGGGGVAYVDQVCSQQWGYGISGIDGSGINNFPTYTWDV
                     NLVAHECGHTLGSPHTHWCGWVGGPIDNCNVCWGITTDGGCANGPAPPNNGGTIMSYC
                     HGCTQGVNFVNGFGFQPSSRIRNSIANAGCVNVCTSCNTNLTLSGTHLSTEQQSWWAT
                     NEIIGVNVLQQSGSYLNFNAGVDVILQPNFEVLAGATFHASVGCTPLALPPVIINNAK
                     ALAIKESVLGNDLVVSPNPFSNQLRVAFELNDAAMVTASIIDQTGREIQQTVLNQQME
                     KGRQEVNMNTNNISAGIYFLVVSINNVKYTRKIIKM"
     assembly_gap    6191..6202
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6203..>6523)
                     /locus_tag="PK736_05835"
     CDS             complement(<6203..>6523)
                     /locus_tag="PK736_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32945.1"
                     /translation="AAFEANYKSIRLMIPAGNNKTIAVLLTQHDLYGSDFELSITDEY
                     GKHTSPINKGRYYFGHVENETNTLAAISIYENEIGGVISNNEGNWNLGKLEKFTDKYG
                     YYND"
BASE COUNT         1813 a         1418 c         1172 g         2108 t
ORIGIN      
        1 tcaaatacaa aacgtcagaa atgttgaaaa tttaaagcaa tgaattaaat ccttgtttgt
       61 tcggcttgct tcatattcgc ttaccattgg ccctgccagt gtacctgttt gcttcgatag
      121 caaattatta atggcacatc cctctatatt tgcattaaaa gtcatgctat tgccaacaaa
      181 aaatcctgga tttaactgaa tatacgtttg tgctttatag gtaacggctg cgccattgcc
      241 ccacgtcata ttatcggtag aggtaacggt gccagcacca tgccagtact ctgcagccga
      301 tgatgggctt gtattatcaa gaaacaagtt gcccaaacaa agttgaggaa gcggtgtgcg
      361 ccataagcca cggctataag tgcccgctac taatgtatta gttgctgcat atattttcaa
      421 gtcggttaca attacattgg gcaaaccatt gttgtaggct gtccatgggc cgcttgtatt
      481 caaatcatac atatacactc ccacatctgt acctacaaaa agataattat tataagaggg
      541 atgaaatgcc gcacagttta ttggcacatt tggcaaaccc gtatttaacg gtatccaagt
      601 aaccccacca tcgttgctat acactacttt tttattcgga ttatatcctc ccattgttag
      661 caaagcttga gtggcattgc gttcgctaaa cacaacattt gtaattggga aggcaatatc
      721 tgtatcgttt acaaaagtaa aagttggcaa agcagccaat gcatttgcag tgcggtctat
      781 ccaactgtat tgaattaccc gtatcatttg ttggttattg attgtgctga gcgcaagttt
      841 cgaaacaagg tatggtaaac tatctgttat ggcattccaa gtatcgcctt tatcattact
      901 acggaatatt tttcggtacc cggcatacaa tctattttca ttttgtttat ctaatatgaa
      961 gggcgtaaaa aataaaccat cacccgaatt tggtggacta atgtagcttt ccaatgccga
     1021 tgtaccacca ctatcgcttc taaatatgcc accaccttgc cagctataat atattacatc
     1081 tttgttagat ggttgtatgg ccacttgcat accatcggcc ccttttacat gtaagttggt
     1141 attagcacct acatcaatgt agttgcagcc attatcctgc gcaccggcca ttacataatt
     1201 attattgcct tgcgatacat caatagagta atattgcgaa atatttaagc ccgtacttat
     1261 atcttcccaa gtgctgccat tattggtact tagaaaaatt cccccatcgc tgcatgcata
     1321 caatacatta tcaccatctg taaattttaa atcgtgaata tcagcatgca cataatttgt
     1381 atcgttagca ttttgcaccc agtgcgatga taatgtccat gtagctccgg tatcaacact
     1441 tcgccataca tttataccgc ctactattac aattgatggg tcggtattgt gcactgccaa
     1501 tgctaaatca taaccacttt gtgccgcgct atcaccactg ctgccatcgt cttgcgcgca
     1561 taatatagca ggtgaattcg acttgaaact aaaatttgct gcactattat tagaaacata
     1621 aaagcctcta aagccaccaa tcttacctgc ctggcccagc aaagcatata ctctatttgg
     1681 tgaggctggg ctaaccgcta tggctatacg cgctatgttg ctacctggca agcctgcagc
     1741 cgtgttccaa gtactgccgc tattggtgct atattttact acggtatcta tcgcacaata
     1801 tacggttgac gaattacccg gtttaaactc tgcatcgcgc gcattgcctg caagaactaa
     1861 cgtccaggtt gatgcggcat tatcggtttt gtaaacgcca aacgatgtta cagcatataa
     1921 cacatttgaa ttagtagggt ccatcattag tttgtatcca tttacctgct gcacaaccgt
     1981 aaaagataat cctgttgtgt tccatgtaag gccaccattg atactcttaa gcacacctac
     2041 agattttgta ttgcctccat cgccatcacc ggtaagaata taaatagtgt tactgattgt
     2101 aggatgcaca catattccac ttacgcctaa taccggcagc ccattgctaa tgctattcca
     2161 tgagttgcca ccggtatttg ttttccatat accaccagcg ggcgtaccta cccaaaactc
     2221 cgatgcacca aacgagttgc cttgaaatgc taccgtattt acacggccta taccaccagc
     2281 ataacctgta agcccaagcg taaaattagt aggccccatc gattgccaat ttgctaccgg
     2341 tgccatcatt gctttttgtg atgcgttatt ttttattgca agctcatgcg catagtatga
     2401 ttcccacgtt ttagccgttg agtttataat ccgtttgttg ccatccaatc gcatttgtgt
     2461 atcatgtttc cacctgctat attgcacata gcccgagcca cgttgtttgc cattggtctc
     2521 gtaccaggca tcatacactt tgcatatcga atcaaagctg tcgcctactt ttatatcact
     2581 taaatcgcgc ctaaagttgc gctcttgggc cgacatgtgc atgtacatgc agcaccacat
     2641 tattattaat attatttttt tcatttctgt ttttgagttt tttgtttgtt taaaataatt
     2701 tgggcgtgcc cccgaaaaaa atcgtgtttt ttatttttgc cttttttcga ggtcgggctg
     2761 tacgctacta tcttttgttt taagaggttt gtgtcctcac aaatcacccg gtaaaacaaa
     2821 aggatatacg ctgccatccc tcacgctttg caccgctacc aacggtagtg gcctatgccg
     2881 tcagatttat tttttttcca acaattttac tcgctcaacc aatgcagcat tattattttt
     2941 caattgctca ttcgctttat tcaactctat tacatacaag gtaagttctt ctactttttc
     3001 aactaatttc atttgcaatt gacctaccgt tacttcttgc tcctctacct cagtggctgt
     3061 tggcatacca ggcagatgat tatttttgtt tacataattt tccaattcat taagtggaag
     3121 caatttgtaa tcattggcaa atacataatc gggaaatggt tgcaactgca cccgcaaatc
     3181 ggtacagata atactaccac gcaccgtaag cttatagttg ctacttgcat caccacctat
     3241 agcaacacgg ccatcgcctt gtatgcgcat tagctcgttg cttgatgtgg aatctactgc
     3301 cgcataaaaa acatggttgc cgcccaaatg ttctacttga tatcgaagtg tattggcatt
     3361 cataccaaag cctgcaaatt ggtgttcgtt atcgacacct tcccaaagta caattctgcg
     3421 gttattcaaa ccattatcaa actgcaattg ggcatgtggg ttcgatgagc caaaacccac
     3481 attgccattt tgcaacagtg ttatttgcgg ccaattatta ttggtcatga agtacattgg
     3541 gtgatcggat tctgtgccaa gccatccacc ggctaagtta ttatatacat ttaaaccaat
     3601 gtaagttccc attttaacag tgccatccgt gtgtacaatt ccataaactt ctgattgtgt
     3661 ttgcactgta agtttcgcgc ttgggcttgt ggtgcctata cccacgcttc cttgattatc
     3721 aatcatcatg cgtggctgta ttagcaaatt gttatcaccc accgaaaaaa gcatacctgc
     3781 tccattgcca ctactattca atatacttgt aattttacct atattgctgc caaagccacc
     3841 ataaaaacca atagaatttg ttgcccctcc aaaattccca gtattaatta atttcaatgg
     3901 ctcgtactta tcgtaagagg ttgcggtggt attactgtta agtatcaaag aattgaaagt
     3961 ggtgttttgt ggtgcggtat taattcggca gtcaccacta atatcaagtt tgaatgatgg
     4021 tgaagcatta cctattccaa ccttaccgcc ttgtgtacat agtgccatgt tatcgttagc
     4081 aaggtcaatt cgtaatggtg tggtgttgag gcttacattt ttaatattaa aattattatt
     4141 gccaagcata ccgctgttcc ataaggttgt tccaagagtt ttgtaagcaa aagctgcatt
     4201 ggccgcagca cttcctttat ccataaacat agtggcacta agtgtagagg atttaatacc
     4261 aagattaaca gcagctgttt tctcagcata aatgttggca ccatttacgg tagtgcctac
     4321 gcttacattg tcggttaaca ccgtaggggc aatcacagca cctgtttttg tccattgcgc
     4381 ttgcgcctgc aaagccacaa cacatgcagc aattgcgaga atcatttttg tttttcttcc
     4441 cgaattgcgg gattttactt tgttcaacat ttttttgaaa ttttttaaat attaatttat
     4501 attgtttaga actctttttt ttggaaccaa tagccaatca gcaacattca gaacttgtat
     4561 tctattctta tcgaaattca aaatcaattc tcaattttga taaaagcaaa aaaatttttt
     4621 tctttataaa acttacaact tcggttttaa attttccttt tagtattgag ccaggaatgc
     4681 aacttttatt aaagacttaa tgatggtact attaggtacg gcaaatatta aaaactaaag
     4741 tcgattccac aatccctaat attagggata aagatttttt ggcattgtaa aaaatagtgt
     4801 aacggacttt tttatttgaa tggtggtgaa aatataatgc aaaaaaaatc ctactcacag
     4861 gagtaggatt tttttggatt aggaatgttt tatttttttg agatcacatc tttataatct
     4921 ttcttgtata cttaacatta ttgatgctca ctactaaaaa gtaaatgcca gccgaaatat
     4981 tatttgtgtt catattcact tcttgccggc ctttttccat ttgttgattc aatactgtct
     5041 gttgtatttc tcgtcctgtt tggtctataa tacttgctgt aaccatagct gcatcattca
     5101 attcaaatgc cactcttaat tgatttgaaa atggatttgg gcttaccact aaatcattac
     5161 ctaatacaga ttcttttatt gctaacgcct ttgcattatt aattattact ggaggtaacg
     5221 ctaaaggtgt acatccaacc gaagcatgaa atgtagcacc ggcaagcact tcgaaatttg
     5281 gttgcaatat gacatctaca ccggcattaa aattcaaata gctgccgctt tgctgcaaca
     5341 catttacacc aataatttca ttggtagccc accagctttg ttgctctgtt gaaagatgcg
     5401 tgcccgaaag tgtaaggttg gtattgcaag aagtacaaac atttacacag ccggcatttg
     5461 ctatgctgtt gcgtatacgt gacgatggct gaaagccaaa accattcaca aaatttaccc
     5521 cttgcgtaca tccatggcaa tagctcatta tagtgccacc attgtttggt ggtgcaggtc
     5581 cattggcaca gcctccatcg gtggtaatgc cccagcacac attgcaatta tcgatagggc
     5641 cgcctaccca accgcaccaa tgtgtatgtg ggctgccaag tgtgtggccg cactcgtgtg
     5701 ccaccaaatt aacatcccat gtatacgtag gaaaattatt gatgcccgag ccgtcaatgc
     5761 cgctaatacc ataaccccat tgctggctgc aaacttggtc aacatacgct acaccgccac
     5821 caccaccaat acttgtgctt agaaatgctg caatactacc ggggtatgta ttggttgtgt
     5881 aataggtacg aaaagtgttt agtgcattgc caccactgct atggtcaaat ggatctacaa
     5941 ctgtccaaat atgtatagcc gaaatgccta agctgatact atcgttatag tagatagtgc
     6001 taataacatt gaacaaacta tttgtgtagt tggttacatt ggtagtattg cttcctttat
     6061 tttgataata cgaaaaactg caatcaaaaa atattttggc acaattgcct tcgtactttg
     6121 ttgtaaatgt ttggttacca ccaccacctt tagcattgaa tgaatcgaag tcaatagtaa
     6181 cgcagataaa nnnnnnnnnn nncatcgtta taatagccat atttatcggt aaatttttcg
     6241 agcttgccca agttccaatt gccttcgtta tttgaaatta ctccgccaat ttcgttttcg
     6301 taaatggaaa ttgctgccag tgtattcgtt tcattttcta catgaccaaa ataatatcgc
     6361 cccttattta tgggtgatgt gtgctttcca tattcatcgg taatggagag ttcaaaatcg
     6421 ctgccatata aatcgtgctg cgtaagtagc acagcaattg ttttgttgtt tccggcaggt
     6481 atcatcaagc gtatcgattt gtaattggcc tcgaacgcag ctt
//
LOCUS       DAORQA010000087         6437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3078_length_6437_cov_10.921185, whole genome shotgun sequence.
ACCESSION   DAORQA010000087 DAORQA010000000
VERSION     DAORQA010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3078_length_6437_cov_10.921185"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    376..417
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            620..1810
                     /locus_tag="PK736_05840"
     CDS             620..1810
                     /locus_tag="PK736_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32946.1"
                     /translation="MKRIFLPFTIFAITMMLLSCNASKRALRNGDYDRSVYLSWKKVK
                     RNANKQKQVLVLEEAFKKAQQRDMEEISLLKQENKPENWDMIYELYNGIRRRQTKVKS
                     LPQLYVKKENNRKVVFPQIDVDAQLLEAKKGAANYSYERALKYLSTKNRYDARKAYYE
                     LSGISKYYKDFAATDSLKNYAYNAGLAYVLVQAKNQTGMPLPPGYEDELKKLNINELN
                     RKWSIYETREAQNGSYDYIVSMNVKRIDASPERLSENRYVDKKTIQDGWEYVLDKNGN
                     VMKDSLGNDIKNPKYKTIFCEVIERIQQKSVMVTGQVEFINLSNKQVIKSVPISSENR
                     FDFRSAIAQGDLNALSPESLEKIKNRLPAPFPPTGSMVAATLPLYKQLSVQAIRDNGS
                     LLDD"
     gene            1852..2844
                     /locus_tag="PK736_05845"
     CDS             1852..2844
                     /locus_tag="PK736_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HRC32947.1"
                     /translation="MLRLTSRSRLSNLVATSVSFYFSKWTRSTYHWGLPVSIAIEPTT
                     SCNLRCPECPSGLRSFSRATGMIDTALFEKVISQLQKKISYLTFYFQGEPYLHKQFLQ
                     MVSYAVSKKIYTATSTNGHYLTPEMAEATVKSGLHRLIISIDGTTQDVYEQYRKGGDL
                     AKVLQGAKNVIAARRKLQSYTPHVIFQFLVVKPNEHQINEVQKIAGEIGVDEVRFKTA
                     QVYDFENGNPLIPTVEKYARYKQNPNGTYKIKSSLDDNCRKLWHSCVITWNGNVVPCC
                     FDKDAKYVMGNVQQNNFESIWNNDAYKNFRQSLFASRKSIDICTNCTEGAQVWA"
     gene            3310..4830
                     /locus_tag="PK736_05850"
     CDS             3310..4830
                     /locus_tag="PK736_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRC32948.1"
                     /translation="MPIINSVLAWIIKKRIHQIELFLKYPNDIQQESNRKLISAARNT
                     EFGRMYDFASIQNEKEFRNRIPIQNYETLKPYIKRLMMGETNLLWRGETKWFAKSSGT
                     TSDKSKFIPVTTESMEECHFKGGKDLLAIYLNNNPNSQLFNGKLLTLGGSHQVNAFSN
                     ESYYGDLSAIMMENLPVWIELFRTPDLSIALLDEWETKIDKMAHATIAENVTNISGVP
                     SWTLVLLKHILKITGKQNISEVWPNLELMVHGAVNFEPYREQFHQLIPHRGMNYLETY
                     NASEGFFGIQDLSDSNDMLLMLDYGIYYEFIPEKNLSFNHEQAIALADVETGVNYALI
                     ISTNGGLWRYMIGDTIMFTSTNPYRIRITGRTAHFINAFGEEMIIDNAEKAITYACAR
                     TNSIIVDYTAAPIYFDESHNGTHEWLIEFENPPEDIHFFTHLLDEKLQELNSDYEAKR
                     YLNKVMSKPIVRILPRYTFYNWLKMNDKLGGQHKVPRLSNNRKVVDQIRTITSHVH"
     gene            4846..5667
                     /locus_tag="PK736_05855"
     CDS             4846..5667
                     /locus_tag="PK736_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32949.1"
                     /translation="MVIQKRIIISFAFFVAFVFHNAHAQNMKLMYKAKVKAERLITDR
                     LGNFYLIDGSKLTKYNPDGMLLFIYDENSYGNIAQIDAFDPFKIMLYYPAFMHTVFLN
                     EQLTIINQFDLATNNGLGVVSVMCATNENNYWLFDTNIKRLRRLERTGKIIAEGTFMQ
                     SDSMFQLNNLQKIEDTEMYLFMSDQNSNLSRFDKFGNFISTYAMGADIMDYQGSGSHV
                     YFLQKDNIIHSYDFNDFTETVMQVPDDIVALHFRIEKNKLFILTDTEVRIYLLTE"
     gene            complement(5677..>6437)
                     /locus_tag="PK736_05860"
     CDS             complement(5677..>6437)
                     /locus_tag="PK736_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32950.1"
                     /translation="TTFLDVIKVLGVNYILGANNSLKIFVNQRNLSLLNTVGLKNLTT
                     LPDYADVNKTLFGASFIKDVTDYRYSPQRGYTLTVTFAAGKRKIEKNNAFNPELYDSL
                     PLKSNEYKAEIAGDAYFKLMKKLILNVGVTGAKLQTENTFNNELFRFGGLKTLRGFDE
                     ESFNANAYIIGKTELRLLLDGNSYLFAFYNQAYYEQRSFDVKRSDTPLGFGAGLAFET
                     KAGIFSFNYALGKQQNNPILFRSAKVHFGIVSAF"
BASE COUNT         2138 a         1138 c         1200 g         1919 t
ORIGIN      
        1 aatacttttt gtcgagaaca aaaataaaag taagcctagt gtaaataatt ttttcatact
       61 gaaagtgttt aaaataataa tggtacaaat attaatttgt tagcattgtt ttgaaaataa
      121 gtaattaaaa aaatcaaaaa ctatcaccat tcataatcaa gtaattttta tcaaattttt
      181 tgtattgcaa attttaattc taatatgcac aaatgtaaat aatattctta ctaaaaccta
      241 ttgcctcaaa tttcaagcct atttgaatgt acagatgtaa aagcgtccct taaacttgct
      301 tttgatgatg gttgttaatt aaatcaaacc acgacttcaa caaatggaaa ctaatttcac
      361 ttacaatatc tttcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg
      421 aacaaattta catcaataag aaataagttt tttgaacggt tctttatcca gctgaacgta
      481 tccgctaaaa tcgtttattt cagttacaat cttaatgcga tttatttttt aaaattccca
      541 tttcatatat cattcacaag tccttatcat tgtggctaat attttgaata acacattata
      601 tgattcaaaa cataaaatta tgaaaaggat ttttttacca tttaccattt ttgctatcac
      661 tatgatgctg ttgtcgtgca atgcctctaa acgtgcatta cgcaatggtg actacgaccg
      721 ttcggtatat ttatcgtgga agaaagtaaa gcgcaatgcc aacaaacaaa agcaagtgct
      781 ggtgcttgaa gaggcattta aaaaagcgca acaacgcgat atggaagaaa tatcgttgct
      841 gaagcaagaa aacaaacccg aaaattggga catgatttat gaactgtata atggcataag
      901 gcgcagacaa acaaaagtaa aaagcttgcc acagttgtat gtaaaaaaag aaaacaaccg
      961 caaagttgtg tttccgcaaa ttgatgttga cgcacaattg ttagaggcaa aaaaaggtgc
     1021 ggcaaattac agttatgaaa gggccttaaa atatttatcc acaaaaaata ggtatgatgc
     1081 acgcaaggca tactatgaat tgagtggtat aagtaaatat tataaagact ttgctgcaac
     1141 cgatagttta aaaaattatg cctacaatgc cggcctggct tatgtgttag tgcaggcaaa
     1201 aaatcaaaca ggcatgccat taccacccgg ttacgaagac gagttgaaga aattaaacat
     1261 caacgaactc aaccgtaagt ggagtatata cgaaacacgc gaagcacaaa atggaagtta
     1321 tgattatatt gtaagcatga atgtaaagcg catagatgca tcgcccgaac ggctgagcga
     1381 aaatcgttat gttgacaaaa aaacaataca agatgggtgg gaatatgtgt tggacaaaaa
     1441 cggaaatgtg atgaaagata gtttgggtaa cgatattaag aacccaaaat acaaaactat
     1501 tttttgcgaa gtgatagaac gtatacagca aaaaagtgta atggtaacag ggcaggttga
     1561 gtttatcaat ctttcaaata agcaagtaat aaaaagtgta cccatatcga gcgaaaatag
     1621 atttgatttt cgcagcgcga ttgcacaagg tgatttgaat gccttatcgc ctgagtcgtt
     1681 agaaaaaata aaaaatagat tgcctgctcc gtttccacca acgggaagta tggttgctgc
     1741 cacattgcca ctttataaac aactttcggt gcaggccatt cgcgataatg gctcgctttt
     1801 ggatgattaa tttttgctat gaacattcaa ttgcttcgcg ataaatttca aatgttacgc
     1861 cttacatcgc ggtcgcggtt gagtaatctt gttgctactt cagtatcatt ttattttagc
     1921 aagtggactc gttccacata ccactgggga ttgccggtga gtattgcaat tgagccaaca
     1981 acatcatgca acttgcgctg ccccgaatgc ccaagtgggt tacgttcttt ttcgcgtgct
     2041 acaggtatga ttgataccgc attgttcgag aaggtaattt cgcaattgca aaaaaaaata
     2101 tcctatctca ctttttattt tcaaggggag ccctatttac ataagcaatt tttacaaatg
     2161 gtatcatatg ctgtaagcaa aaaaatatat accgcaacaa gtaccaacgg gcattacctc
     2221 actcccgaaa tggcggaggc tacagtaaag tccggattgc acaggcttat catttctatc
     2281 gatggcacta cacaggatgt gtatgaacaa taccgcaaag ggggtgactt ggccaaagta
     2341 ttgcagggtg caaaaaatgt aatagccgcc aggcgaaaat tacaatcgta tacaccccat
     2401 gttatatttc agtttttggt agtgaagcca aacgaacatc aaataaatga agtacaaaaa
     2461 attgccggtg aaataggagt ggatgaagtg cgatttaaaa ctgcacaagt gtatgatttt
     2521 gaaaatggaa acccgcttat tcccaccgtt gaaaaatatg ctcgttataa acaaaaccca
     2581 aatggcactt acaaaataaa aagctccctc gatgataatt gccgcaagtt gtggcactcg
     2641 tgtgtaatta cctggaatgg aaatgtggtg ccatgttgtt tcgataaaga tgcaaaatat
     2701 gtaatgggaa atgtgcagca aaataatttt gaaagtatat ggaacaatga tgcgtataaa
     2761 aattttcggc aatccttgtt cgcctcgcgc aagtcaattg atatttgtac caattgcacc
     2821 gaaggggcac aagtgtgggc ttgatgagtt aattaaaact gactgttgtt ttcaccaacc
     2881 aaaaacttca ccgacaaagt cattcaggaa gaaccttttt gtataaacgg aaatttcatt
     2941 ttgtaataat ttatctaaag tagaaataag tttcgtcctg aatgacttgg ggtgatgaac
     3001 aaatttaacc aaagtagaaa taagtttcct catgaaggac ttttggtgat gcacaaattt
     3061 atttaaagta gaagttagtt tcctcctgaa ggacttgggg tgatttacaa atttatctga
     3121 aatagaaata aaattcctct tgaaagaatg tgtccctaaa cttcttctag gagaaatctt
     3181 tttgtgttca cgaaaatttt cttctcattt cctctttttc atatgtggtt gaaaacaccc
     3241 ggcaaattat tttttttcat atcacattat tcaaaatttt tccttttctt cattgcatta
     3301 atttgagtca tgccaataat aaattctgtt ttagcctgga taataaaaaa gcgcattcat
     3361 caaattgaat tgtttttaaa atatccaaac gatatacaac aagaaagcaa ccgcaagttg
     3421 atttcagctg cacgcaatac agagtttggt cgcatgtatg attttgcttc tattcaaaat
     3481 gaaaaggaat ttcgtaatcg catacctatt caaaattacg aaacattgaa gccttatata
     3541 aaaaggctga tgatgggcga aacaaatttg ctgtggcgtg gcgaaacaaa atggtttgca
     3601 aaatcatcgg gcactaccag cgacaagagt aaatttattc ccgtgacaac ggaaagtatg
     3661 gaagaatgcc actttaaagg cggtaaagat ttgcttgcca tttacctcaa caataatccc
     3721 aactcgcaac tcttcaatgg aaagttactt acactcggag ggtcgcatca ggtaaatgct
     3781 tttagtaacg aaagttatta tggcgactta agtgcaatca tgatggaaaa tttacctgtg
     3841 tggattgagt tgtttcgcac gcccgattta tccatcgcat tactcgatga gtgggaaaca
     3901 aaaatcgata aaatggcgca tgctaccatt gccgaaaatg taaccaacat aagtggtgtg
     3961 ccttcgtgga ctttagtttt gttaaaacac attttaaaaa ttaccggcaa gcaaaacatt
     4021 agcgaagtgt ggcccaacct cgaactgatg gtgcatggtg ctgtaaattt tgaaccctac
     4081 cgcgagcagt ttcatcagct tattccgcac cgtggcatga attatttgga aacatacaat
     4141 gcttcggaag gattttttgg aattcaggat ttaagtgaca gcaacgatat gttgctcatg
     4201 ctcgattacg gaatttatta cgaatttatt cccgaaaaaa atctttcctt caatcatgag
     4261 caggccattg cacttgctga tgtggaaacg ggcgtaaact atgccctcat aataagcacc
     4321 aatggcggat tgtggcgata tatgattggc gataccatca tgtttacttc tactaaccct
     4381 taccgtatac gtattaccgg ccgtaccgca catttcataa atgcctttgg cgaagaaatg
     4441 ataattgata atgccgaaaa agcaattact tatgcttgtg cccgcaccaa ttcaattatt
     4501 gttgattaca ctgccgcccc catttatttt gacgagtcgc acaatggcac acacgagtgg
     4561 ctcatagaat ttgaaaatcc gcccgaagac attcactttt ttacgcacct tcttgatgag
     4621 aaattgcagg aactgaactc cgattacgag gcaaaacgat acctcaacaa agtaatgagc
     4681 aagccaatag ttcgcatctt accacgttat acattttata attggttaaa aatgaacgat
     4741 aaactcggtg gtcagcacaa agtgccaagg ctaagcaata accgaaaagt ggtagaccaa
     4801 atacgcacca taacatcgca tgtacattag tctttagttc ataaaatggt tatacaaaag
     4861 agaataatta tttcgttcgc attttttgtt gcatttgttt ttcataatgc ccatgcgcag
     4921 aatatgaaac tgatgtacaa ggcaaaagta aaagccgaaa ggttgattac agataggctg
     4981 ggaaattttt atttgataga tggaagtaaa ctcactaagt ataatcctga tggaatgttg
     5041 ttgtttatat atgacgaaaa cagttatggc aacattgcac aaatagatgc tttcgatcct
     5101 ttcaaaatta tgctttacta cccggctttt atgcacactg tttttttgaa tgagcaactg
     5161 accatcatca atcaatttga ccttgctaca aataacgggt taggtgtggt aagcgtaatg
     5221 tgtgccacca acgaaaataa ttattggtta ttcgatacaa atattaaacg gctaagaaga
     5281 cttgaacgaa caggaaaaat tattgccgaa ggaacattta tgcaatccga ttcgatgttt
     5341 cagttgaaca atttgcaaaa gattgaagat acagaaatgt atttatttat gagcgaccaa
     5401 aatagtaact tgtctcgttt cgataagttt ggaaatttta tcagcactta cgctatgggt
     5461 gccgacatta tggactatca gggttcaggt agccatgtgt actttttgca gaaagataat
     5521 attattcaca gttatgattt taacgacttt accgaaactg ttatgcaagt tcctgatgat
     5581 attgttgcac tgcacttccg tatcgaaaaa aacaaactct tcatacttac cgacactgag
     5641 gtgagaattt atttattaac cgaatagata gtatttttaa aaagcactta caatcccaaa
     5701 atgcactttt gcactgcgaa ataaaatagg gttattttgt tgtttcccta atgcataatt
     5761 gaatgaaaaa attcctgcct tggtttcaaa ggcaagcccg gcaccaaatc caagcggagt
     5821 atcactgcgt ttcacatcga acgaacgttg ttcatagtat gcctgattgt aaaaagcaaa
     5881 aagataagag ttgccatcaa gtagtaaccg caattctgtt ttgccaatta tgtaagcatt
     5941 ggcattaaaa ctttcctcat caaatccccg gagtgttttt aatccgccaa agcgaaacag
     6001 ttcattgtta aatgtgtttt ctgtttgcaa ttttgctcct gttactccta cattgaggat
     6061 taattttttc attaatttga aataggcatc tcccgcaatt tcggccttgt attcgttact
     6121 tttcaaaggg agagaatcat acaactccgg attgaaggca ttgttttttt ctattttgcg
     6181 tttgcctgct gcaaaggtaa cggtgagtgt ataccctcgt tgtggactgt aacggtaatc
     6241 ggtaacatct tttataaagc ttgccccaaa taaagtttta tttacatcgg cataatcggg
     6301 tagggttgtc aggtttttta atcccactgt attgagcaac gataagttgc gctgattgac
     6361 aaatatcttc aaactattat ttgctccgag aatataatta acgcccagta ctttaattac
     6421 atcaagaaag gtggtgt
//
LOCUS       DAORQA010000088         6420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3088_length_6420_cov_6.638963, whole genome shotgun sequence.
ACCESSION   DAORQA010000088 DAORQA010000000
VERSION     DAORQA010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3088_length_6420_cov_6.638963"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2329)
                     /gene="priA"
                     /locus_tag="PK736_05865"
     CDS             complement(<1..2329)
                     /gene="priA"
                     /locus_tag="PK736_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRC32951.1"
                     /translation="MYIEIILPLYLIKEYTYHVPDDLADQVMPGKRVLVQFGKSKIYA
                     GLIKSIVAKVPTGYEPKSILEILDEYPIVNAQQLKLWQWIADYYLCTLGEVMLCGLPS
                     SLKLESTAIVSLHPAFIETETTLTDREFLIVEALHSVANLEIKEVEKIVGTKAVFKLL
                     KTMVEKNIIQLNQEIDTASKARKVVYVELAEKYKDENNLRDLFSSLDLKAPKQFELLL
                     HYLNITMNRNAEKAIPKTKLIKTANSSSAIFNQMHKKEIFVTIELADFDIAKPEKENA
                     PSSILSPEQITAYEEIMTQHTKKDIVLLHGVTSSGKTEIYIKLIENCVRNYKQVMYLL
                     PEIALTTQMIKRLQKHFGDKLLVYHSLYNETERLLTWNKMLKYKRDTENRIFDFQIVI
                     GARSALFLPYSNLGLVIIDEEHEPSFKQYDPAPRYHARDAAMVLAKYFEASVVLGSAT
                     PSIETYYNAKAGKFGLVELNSRFGNLELPSISIVDLKEEYKRKKMKGFFSQAFLTGVS
                     NALAANEQVILFQNRRGFATMLECKQCGWAPQCINCDVTLSYHKINNQLSCHYCGYKM
                     SVPQKCMACSSPQLETKGIGTERIEEEMEILFPEKKIARLDLDTTRTKNAYRKILSAF
                     EEGETDILVGTQMITKGLDFDNVSTVGIVNADTMLNFPDFRAAERSLQLMLQVSGRSG
                     RKNKQGKVIIQSYRPSHPVLQYVLAHDYLGFYNAELKERKLFGFPPFTKLIEITLKHK
                     NLRTCDEGAIYYTKILKDQFAQRVLGPSIPHVSKIS"
     gene            complement(2454..4952)
                     /locus_tag="PK736_05870"
     CDS             complement(2454..4952)
                     /locus_tag="PK736_05870"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRC32952.1"
                     /translation="MASALIFSFHSKAQVVINEYSCSNLTQFVDDHSDYGDWFELYNT
                     GNTAVSLQGMYLSDDSLNNLKWLVPANVNIGAFGFARFWASGRNAVSGTHFHTNFKLT
                     QTKTNKEFIVLSDANGVIQDYVKLTNETKLGHSIGRTQNGGATWGIFTSPTPNGSNNG
                     ATSYQRYAARPDFSLLAGHYPASITVDITTTEPNATIRYTIDGNEPTGASLLYNGPVT
                     IPTTKVLKAISISPDPQVLTSLMRFDTYFINVSHTLPIVSVSATSLTNLANGNGSLEP
                     HGALEYFDTAGVRQAKTYGRFNKHGQDSWVLSQRSLDFAARDEMGYNYTIKEQLFATT
                     TRDEFQKIILRASGDDNYPADHNNANAGSAHMRDAYVHMLAKNGGMNLDVRTAAKCIV
                     YLNGAYWGVYDIRDNPDDHDYTNYYYGQDKYHLQFIETWGNTWSHYGGAQTLTDWGAL
                     KSYIYGNSMADPAKFQYVTDRYDVTSLVDYVITNTFTVCTDWLNYNTGWWRGMDSTGT
                     HLKWGYILWDNDATFDFYINYTNVPSTTANSAPCGVENSSSVSDPQNHIDILNKLRVN
                     PDFNQYYISRQIDMWNTVFSCDNMLGQLDSVIAVIDPEMAQHSLRWAGTYTEWVTNRN
                     ILRSFIQNRCILLETGFKNCYNLTGGYDITLNTDPAGAGDIKLNTLTHTQLPWTGKYF
                     GGMENLIAVMPKPNYNFVNWSSASQTFSPNVNATNVKVDFNANDSVIAHFTLANGIFE
                     LEGSMPNISAMPSVFNSATNLEYYLPENAAVNIDLISASGQKVMTLSQMENQPKGHYM
                     IHLNLAGTTLPSGVYFVNMIAGDHKSTVKLIYSK"
     gene            complement(5146..5523)
                     /locus_tag="PK736_05875"
     CDS             complement(5146..5523)
                     /locus_tag="PK736_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRC32953.1"
                     /translation="MKKIIFTENAPKPIGPYSQAILLNDVLYASGQIAINPENDQLVL
                     DNIIEETIQVMKNIGALLTAAGMDYTHIIKTSIFLKDMNNFSQVNNIYGSFFKGDFPA
                     RETVQVARLPKDVNVEISVIASF"
     gene            5595..6164
                     /locus_tag="PK736_05880"
     CDS             5595..6164
                     /locus_tag="PK736_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRC32954.1"
                     /translation="MKILLLDNYDSFTFNLLHYLQKYDGVVVDVFRNDAITVKEVAVY
                     DAIVLSPGPGLPEQSGIMMELINEYKSQKKILGVCLGLQAIAVSFGYELYNLETVQHG
                     VARKTFLTAPDSLFDEIPQDFICGRYHSWVVKNNDDNKNLITTATDESNYIMAMKHSE
                     YNLRGVQFHPESILTEHGDQMMYNWLFKC"
BASE COUNT         1886 a         1223 c         1205 g         2106 t
ORIGIN      
        1 tgctgatttt ggaaacatgc ggaatgcttg gccccagcac cctttgcgca aattgatctt
       61 tcaatatttt tgtataataa attgcacctt catcgcatgt tcttaggttt ttgtgcttta
      121 aagtaatttc aataagtttg gtaaaaggag gaaaaccgaa aagctttcgt tcctttaatt
      181 ctgcattata aaagcccaaa tagtcgtgcg ccaaaacata ttgtaacacc ggatgtgaag
      241 gccggtatga ttgaataatt acttttccct gtttgttttt tcgaccggag cgtccgctta
      301 cttgaagcat caattgcaaa cttctttcag ccgctcgaaa atcaggaaaa ttgagcatag
      361 tatcggcatt cacaattcct acagtactta cgttatcgaa gtcgagccct tttgtaatca
      421 tttgcgtgcc caccaaaata tccgtttccc cttcttcaaa ggccgataat attttgcgat
      481 aagcattttt tgttcgtgtg gtatcgaggt ctaagcgcgc gatttttttt tcgggaaata
      541 atatttccat ttcttcttct atccgctcgg tgccaatacc tttggtttct aactgaggac
      601 tgctgcatgc catgcatttt tgcggtacac tcattttgta accacaataa tggcaactca
      661 gttgattgtt aattttgtgg tagcttaatg ttacatcgca gtttatgcat tgcggtgccc
      721 aaccacattg tttgcattcg agcatggtag caaaccctct tcgattttga aaaagtatga
      781 cttgttcgtt tgcagcaagc gcattgctaa ctcctgttaa aaatgcctgc gaaaaaaaac
      841 ctttcatttt ttttcgtttg tattcttctt tcaaatcgac aatcgaaatt gatggcaatt
      901 ctaaattccc aaatcgcgaa ttcaattcta ctaatccaaa ttttccggct tttgcattgt
      961 aatatgtttc tatagatggt gttgccgaac caagcacaac actagcttcg aaatattttg
     1021 ctaaaaccat tgcagcatcg cgggcgtgat accgtggtgc cggatcatat tgcttaaaac
     1081 ttggttcatg ttcctcatca ataattacaa ggcccaaatt ggagtagggc agaaacaaag
     1141 cagaacgagc cccaattact atttgaaaat cgaatatgcg gttttctgta tcgcgtttat
     1201 acttcagcat tttgttccag gtaagcagac gttcggtttc gttgtacaaa gaatgatata
     1261 ccaaaagttt atcgccaaaa tgtttttgca accgctttat catttgtgtg gtgagcgcaa
     1321 tttcgggaag caaatacatt acttgtttgt agttgcgcac acagttttca ataagcttta
     1381 tatagatttc cgttttaccg ctcgatgtaa ctccatgcaa caacacaata tcctttttgg
     1441 tgtgttgagt cattatttct tcgtaggctg taatttgctc gggtgataat atgcttgaag
     1501 gggcattttc tttttcgggt tttgcaatat cgaagtcggc aagttctatt gttacaaaaa
     1561 tttctttttt atgcatttga ttaaagattg cactactgct atttgccgtt tttatgagct
     1621 tggtttttgg aatcgctttt tctgcattgc gattcatggt gatgtttaaa taatgtaaaa
     1681 gcaattcaaa ctgcttaggt gcttttaaat ccaacgaaga aaataaatcg cgcaggttgt
     1741 tttcatcctt atacttttcg gccagctcaa catatactac tttgcgtgct ttacttgctg
     1801 tatcaatttc ctgattgagt tgaatgatat ttttctccac catggttttc aataatttga
     1861 aaacggcttt ggtaccaact attttttcta cttcttttat ttccagatta gcaacgctat
     1921 gcaacgcttc aacaataaga aattcgcggt cggtcagggt agtttctgtt tctataaatg
     1981 ctggatgcag gcttacaatg gctgtgcttt cgagcttcaa tgatgatggc aatccacaca
     2041 acattacttc gccaagggta cacaaatagt aatcggcaat ccattgccaa agcttcagct
     2101 gctgagcatt cacaatagga tattcatcaa gtatttcgag aatagatttt ggctcatagc
     2161 cagtaggcac cttagcaaca atacttttaa tcaggccggc atatatctta cttttgccaa
     2221 actgcaccag cacacgctta ccgggcatta cctgatctgc cagatcatcg ggcacatgat
     2281 aggtatactc ctttatcaaa tataatggta aaataatttc gatgtacatg gtaaatgaat
     2341 gctgattgca atgatacatt agtcttttcg aaaatcaacc cttgatgaaa agtaaaacaa
     2401 aaaaaatcct gcatcgaatg acacaggatt ctataaaacg tattgaagaa atattatttc
     2461 gagtaaatca attttactgt tgacttgtga tcaccggcaa tcatgtttac aaagtacaca
     2521 cccgatggca aagtggtgcc ggctaaattc aagtgaatca tgtaatgtcc ttttggttgg
     2581 ttttccattt gtgaaagtgt cattactttt tgtcctgagg ccgaaatcaa atcgatattc
     2641 acagcggcat tttcgggcag gtaatactct aaattagtag cgctgttaaa tacgcttggc
     2701 attgcactta tgtttggcat acttccttcc aattcaaaaa ttccatttgc taaagtgaaa
     2761 tgtgcaatca cactatcgtt ggcattgaag tcaactttta cattagtagc atttacgttg
     2821 ggcgaaaaag tttgcgaagc cgatgaccag ttcacaaaat tgtaatttgg ttttggcatt
     2881 acggctatta aattttccat accaccaaag tattttcctg tccaaggaag ttgtgtgtga
     2941 gttagcgtat tcaattttat atcacctgca ccagctgggt cggtattaag tgtaatatca
     3001 taaccgcctg taagattgta gcaattttta aatcctgttt ctaaaagaat gcagcggttt
     3061 tgaataaagc tacgtaatat atttctattt gtaacccatt ctgtataagt acctgcccag
     3121 cgtagtgaat gttgtgccat ttcgggatct attactgcaa ttacactatc caattgacca
     3181 agcatattgt cgcaactaaa taccgtattc cacatatcta tttggcgcga aatatagtat
     3241 tgattgaagt ctggatttac gcgcagttta ttgagtatgt caatatgatt ttgtggatct
     3301 gaaactgacg aagaattttc gactccacac ggtgcgctgt tggcagtagt gctgggcacg
     3361 ttagtatagt tgatgtaaaa atcgaaagtg gcatcattgt cccaaagtat gtagccccac
     3421 tttaaatgtg tgccggtgct gtccattccg cgccaccaac ctgtgttgta attcaaccaa
     3481 tcggtgcata ctgtaaatgt gtttgtaatt acataatcaa caaggcttgt aacatcatat
     3541 ctatctgtca catattgaaa ctttgccggg tcggccatgc tgtttccgta aatataactt
     3601 tttaatgcgc cccaatcagt tagtgtttgt gcgccaccat aatgcgacca agtattgccc
     3661 caagtttcaa taaactgcaa atggtattta tcttggccat agtagtagtt tgtataatcg
     3721 tggtcatctg gattatcacg tatgtcatat acaccccaat aagcaccatt caagtataca
     3781 atgcactttg cagcggtacg cacatccaaa ttcataccgc cattttttgc caacatatgt
     3841 acgtaagcat cgcgcatatg cgcactacct gcatttgcat tgttatggtc ggcaggataa
     3901 ttatcgtcac ctgatgcacg taaaataatt ttttgaaatt cgtccctagt agtagtagca
     3961 aataattgtt ctttgatagt gtagttatat cccatttcat cacgggcagc aaaatccaaa
     4021 cttcgttgac taagtaccca cgaatcctgc ccatgcttat taaatctccc gtacgttttt
     4081 gcttgacgca cacctgcagt gtcaaagtat tccaaggcac catgaggctc caaacttccg
     4141 tttccatttg caaggttggt aagcgatgtt gccgatacag aaacaattgg aagagtgtgg
     4201 ctaacattta taaaataggt atcaaagcgc attaaacttg ttaatacttg tggatcgggc
     4261 gatatgctaa tggcctttaa tactttggta gttggtatgg ttactggccc attgtaaagt
     4321 aatgaagcgc ctgttggttc attgccatca atggtataac gaatggtggc attgggttca
     4381 gtggtagtaa tatcaactgt tatagatgct ggataatgcc cggctaacaa actaaaatca
     4441 ggacgagctg catatcgctg ataagatgtt gctccattgt tggatccatt tggagtaggg
     4501 gaagtaaaaa tgccccaggt tgcaccacca ttttgagtac ggcctatact gtgcccaagc
     4561 ttggtttcat ttgtgagttt tacataatcc tgaattacac catttgcatc ggaaagtaca
     4621 ataaactctt tattggtttt tgtttgcgtc aatttgaaat tggtatggaa atgagtgccc
     4681 gataccgcat tgcgccccga tgcccaaaag cgggcaaaac cgaatgcacc aatatttaca
     4741 ttggcaggta caagccactt caaattattt aaagagtcgt cactcaaata catgccttgc
     4801 aaacttacag cagtgttgcc tgtgttatac aactcgaacc aatcgccata atcgctgtgg
     4861 tcgtcaacaa actgtgttaa attagagcat gaatattcat tgataactac ttgcgctttc
     4921 gagtgaaagc taaatattaa ggctgaagcc aaacaaaaaa acatactttt taatttcata
     4981 gatttttatt tattgattta tttatgcgca aaaataaaaa aagaaacata agtttaacat
     5041 aaaatttaca atatgttaac acacgaatta accctgttca aattgtgtat tgattagttg
     5101 tgtgaaagaa tattttattg cacaaaagtg tatgcaagca aaaatctaaa aagaagcaat
     5161 caccgatatt tcaacattta catcttttgg taagcgggca acttgaactg tttcgcgtgc
     5221 gggaaaatca cctttaaaaa aagagccata tatattatta acctgactaa agttgttcat
     5281 atccttcaaa aaaatacttg ttttgataat gtgcgtataa tccatgccgg ctgctgtaag
     5341 cagtgcacca atatttttca tcacctgtat tgtttcttca attatattat cgagcaccaa
     5401 ttgatcattt tcgggattga tagctatttg acccgaagca tacaatacat catttaataa
     5461 aatggcctga ctatatgggc caattggttt gggcgcattc tcggtaaaaa ttattttctt
     5521 cattttgtta ctttattatt cgaaacaaag ttagttttga agtttcattc tcaaaattta
     5581 tttattcttt tttaatgaaa attcttttgc tcgacaatta cgattcgttc actttcaatt
     5641 tattgcatta cttacaaaag tacgatgggg ttgtggtaga tgtgtttcgc aatgatgcca
     5701 ttaccgtaaa agaggtagcg gtttatgatg caatagtttt atcaccagga cccggactac
     5761 ctgagcaatc cggtataatg atggaattaa ttaatgagta taaatcgcaa aaaaaaatac
     5821 ttggtgtttg ccttggcttg caagccattg ccgtttcgtt cggttatgaa ttatacaatc
     5881 tcgaaacggt gcagcacggt gttgcgcgta aaactttttt gactgcgccc gattctttgt
     5941 ttgatgaaat acctcaagat tttatttgtg gtcgctatca ctcgtgggtg gtgaaaaata
     6001 atgacgataa taaaaattta ataaccactg caactgacga aagcaattac ataatggcga
     6061 tgaagcatag cgaatataat ttgcgtggtg ttcaattcca tcccgaatcg atattaacag
     6121 aacacggtga tcaaatgatg tataactggt tgttcaagtg ttgatttcaa ttctatatat
     6181 tttttaccca aacatttttt tggcgattga gtatggtatt aattggcagt aaccctatga
     6241 cattgagttt agcgtttggg aaacaaaatt ttatgaacca ctttacaaac tttactctga
     6301 caccgtgaaa gtataataga gaaaaaattt ggcgggaaaa aatcaaatca aaaagtacaa
     6361 tcccatagac aagctatttt aatcaagaag tttgaaagca gcaaaatggc gcccctaagg
//
LOCUS       DAORQA010000089         6419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3089_length_6419_cov_21.055154, whole genome shotgun sequence.
ACCESSION   DAORQA010000089 DAORQA010000000
VERSION     DAORQA010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3089_length_6419_cov_21.055154"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2712)
                     /locus_tag="PK736_05885"
     CDS             complement(<1..2712)
                     /locus_tag="PK736_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32955.1"
                     /translation="MKIKPHKILFLLFTVFIFLNKTSAQTVLISPTGDGGFETGATFG
                     TNNWTVVNGTQANKWFVGSVPTGFTGTRCAYISNNGTGTTHYYATGSSSVTHIYRDVV
                     FPPGQTCINLTFSWKNDGEWGYDYLAVYLVNTGTTPVAGTELVTGQLGIDYDSQTAWQ
                     TVALSIPAANAGTTKRLVFSWANDNLFGFQPPAAIDNISLVASNGNVNDEPCNATPLS
                     VGTTCNFIGSTNGCASGSAVAAPGCAGYSGGDVWFSAVVPASGKLNFDSNIGTITDGG
                     MAIYTGTCSSLTQIACDDDASLNGAMPAINLSNLTPGTTVFIRFWEYGNDNNGSFQIC
                     VLDPCPTGPGSCMALLGTGVTNVGGLPYNSGAGTTNLMGDDLTSFNTNVCGSSFYLDG
                     MDRVWIFTPTTSGPLTITLTSTGIWTGLMLYDDCPVGSACYTAPGQCVASAQSSTGNK
                     TLTVCVTANVTYYLVLDCFPTPYFNPYSNLYISAPTGAGAPTNDQPCNATPLPLGVNL
                     NGDNGCTGFTGEPAAPGCWLTPNVRNTVWYSVVPTSSSLKIRTFPGLLKNTQIAVYSG
                     TCGAGLTLVDCNDDAPPCGTSAFQSPAYISQLTLTGLTPGTTYYIVVDGYGDLYGTFS
                     IMAIDGSQSFPPIFGQECSLPNPVCAQSITVGDPGYQAFGTLCDFPGAGTNCLLTGER
                     GSAFYEIQISGTGFLEFNIVPNDWAGAPSTSATDYDFAVWKSAGTGAVNCTDILNGAT
                     PLRCNYSYLGVTGLFSNLTNSAPVAYPNFDGAYEARIPVLGGEKYLLNVSNFSNSTSG
                     FTINFGVASPINYTPNPGFVVWTGGVNTDWFNTGNWGGCAIPTCAIDAIIPPSSANQP
                     VIYAPGAACKSISINPGSSLTINAGFSLSVCGDYLNNGTLTAAATSTV"
     gene            complement(2744..3127)
                     /locus_tag="PK736_05890"
     CDS             complement(2744..3127)
                     /locus_tag="PK736_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32956.1"
                     /translation="MKKYFLFIVCSGIMQMYVAAQKNIAAQPIIKTDTRTGEHIVTSE
                     FLIPAQKKPNSNVHIFNALETLQGKVLSYTIAQNNILIVEHIQSFKPEELLQFFRTQN
                     IDAKYKDPVLNRFLFLNDKGKLVHE"
     gene            complement(3380..4927)
                     /locus_tag="PK736_05895"
     CDS             complement(3380..4927)
                     /locus_tag="PK736_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32957.1"
                     /translation="MKHEINIHNCEAWFLDYHENRLTPEEVLQLLLFLDNHPQFKNAF
                     SHFENLSIENLPIDFPNKNLLLKVDAKMDLKLSAQEEIILANIDSVKLHDAENIEIDA
                     SELEALKNSKLIAGEERFENKTSLYKLDITHENIDDWLIAELEGLLNIAEQNALNNFI
                     ANNPQYADEQFLFASAKLLDEKIALDKSTLYKTNIEHEKIDENLIAYSEGILNYDERR
                     EVEIFLDQNPQYKKDLALYQQSKLNASDIEIYPFKGKLKRAVLFPNATLKYVVSGIAA
                     CLIIAFGLNLVLNNGKGNLADTKNIDTVNANTKSVASKTERKQQEVAETISTETNAII
                     DSIEKNVEAQSNAPFNKNTNATKIRVKQIRNYNATQNLAQVKDTVGQNNLKANDIVSI
                     PKKDTIEESIAENPLKIDTTNNQNGNSIIETQAEIVRGTLVLNTPVADSKRKTIKEYF
                     TTLINKKGSKTTPDKDLEEYELLSGKRKITDAVAWALTKVSGDRLKVTTMYNADDKLA
                     RLKLDRN"
     gene            complement(4932..5417)
                     /locus_tag="PK736_05900"
     CDS             complement(4932..5417)
                     /locus_tag="PK736_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRC32958.1"
                     /translation="MTTNEYNKCVDQYSDGIYRFILHNIRDKEEAQDIVQDTYEKMWI
                     NKDNVNPQKVKSYLFTTAYHTMINKIRRNKYKAPMTEVDFNKHYKEESYTGLKEILHE
                     GLKRLPEIQRTVIMLRDYEGYDYREIGEITQLNESQVKVYIFRARQTLKEFIGSMRNV
                     I"
     gene            complement(5484..6200)
                     /locus_tag="PK736_05905"
     CDS             complement(5484..6200)
                     /locus_tag="PK736_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32959.1"
                     /translation="MDTLNSNWITESHIDFEYKKYMLLGYLQHVNEKFSATELFPHLS
                     SIIEHYKNLKLLQENTLKLRSNFPETLTGIDAAEFKLMHESIMNDDSIMSEIEQIVNF
                     SIPQFEKYMFEGRKIYDFVEHKLIISPVGLTPIRVNEGYLFLRNGGEKATHIYNYQIT
                     LFERHDERYRGIHTHYVCSYEVSITNTFEQLKIELLNSHKQLPNPATFAIESSLALPM
                     ESTLLPIAKRILIRHLQETI"
BASE COUNT         1855 a         1224 c         1190 g         2150 t
ORIGIN      
        1 aactgttgaa gttgccgcag ctgtcagcgt tccgttgttg aggtagtcgc cacacacact
       61 taacgaaaat ccggcattga tagtcaatga tgaaccaggg ttgatgctga tgcttttgca
      121 agctgcacca ggagcataaa taaccggctg attggcagat gagggcggaa taatagcatc
      181 aattgcgcat gttggtatag cacatcctcc ccaattacct gtgttaaacc aatcggtgtt
      241 tacaccaccg gtccaaacca caaaacccgg attaggagta tagttgattg gagaagccac
      301 accaaaattt atggtaaacc ctgaagtgga atttgaaaaa ttgctaacat tgagtaaata
      361 tttttcgcct cctagcacgg gtattctggc ttcgtatgca ccatcaaaat tggggtaggc
      421 aacaggtgca gagtttgtta agttgctgaa cagcccagtt actcctaaat aagaataatt
      481 gcatctaaga ggagtagcgc cattcaaaat atcagtgcaa tttacagctc cagtgccggc
      541 agatttccat acggcaaaat cgtaatcggt tgctgatgtg cttggtgcgc ctgcccaatc
      601 atttggtaca atattaaatt ctaaaaagcc ggtgcccgaa atttgaattt cgtaaaatgc
      661 cgaacctctt tcgccagtga gtaaacaatt agtgcccgcc cccggaaaat cgcacaaggt
      721 accgaatgcc tgatatccgg ggtcacccac agtaatactc tgagcacaca caggatttgg
      781 taggctgcac tcctgaccaa aaataggagg aaaactctgt gagccatcta tagccatgat
      841 gctaaaagtg ccgtataaat cgccataacc atcaaccact atataataag ttgtaccggg
      901 tgttaagcct gtaagtgtta attgtgatat gtaagccggt gattgaaaag ccgatgtacc
      961 acagggtgga gcatcgtcat tacaatctac tagagtaagc ccggcaccgc aagttcctga
     1021 ataaacagct atttgtgtgt tttttaataa gcccggaaaa gttcttattt ttaaacttga
     1081 tgaagtgggt accacactat accaaactgt atttcgcaca tttggtgtta accagcatcc
     1141 tggtgcagca ggctcacctg taaaacctgt acatccatta tcgccattta aatttacacc
     1201 caatggaagt ggcgttgcat tgcatggttg atcgttggtt ggtgcccctg cgcctgtagg
     1261 tgccgaaatg tataagtttg aatatgggtt aaagtaagga gtaggaaaac aatccagtac
     1321 aagatagtag gtaacatttg cagtaacaca aacggtaagt gttttgttac cggttgatga
     1381 ttgagctgat gcaacacatt ggcccggagc agtgtagcac gcagagccca cagggcaatc
     1441 atcgtaaagc attaatcctg tccatatacc tgtagaggta agggtaattg taagtgggcc
     1501 agaggttgta ggcgtaaaaa tccaaacacg atccatacca tcaagataaa aagaactgcc
     1561 acatacattt gtattaaaag atgttaaatc atcgcccata agattggtag tgccggctcc
     1621 tgaattataa ggtaagcctc caacattggt tactccggtc cccaacagcg ccatacaaga
     1681 acccggtccg gttgggcacg ggtccagaac acaaatttga aaacttccgt tgttgtcatt
     1741 accatattcc caaaacctga taaagaccgt tgtgcctgga gtaagattgc ttaaattgat
     1801 tgcaggcatt gccccattca aacttgcatc atcatcacat gctatttgtg tgagcgatga
     1861 acacgttcct gtataaatgg ccattcctcc atcggttatt gttccaatat tagaatcgaa
     1921 atttaatttg cccgatgcag gcacaacagc actaaaccaa acatcaccgc cactatatcc
     1981 ggcacaacct ggagctgcca ctgccgatcc ggatgcacaa ccatttgtag agcctataaa
     2041 attgcatgta gtaccaacgg atagtggtgt tgcattgcaa ggttcatcgt ttacatttcc
     2101 attacttgcc acaagcgata tgttatcgat tgctgcagga ggttgaaaac caaataaatt
     2161 atcgttagcc caactgaaaa cgagacgctt ggtggtgcct gcattagctg caggtatact
     2221 taacgccaca gtttgccacg cagtttgcga atcataatca atacccaatt gtccggtgac
     2281 gagttccgtg ccggcaacag gtgtagtacc tgtgtttaca agatacaccg ccaaataatc
     2341 gtagccccat tcgccatcat ttttccatga gaaagtgagg ttgatgcaag tttgcccggg
     2401 agggaaaacc acatctctgt aaatatgcgt gaccgatgac gatccagttg cataataatg
     2461 tgttgtacct gttccattgt tcgatatgta tgcacaacgg gtgcctgtga accctgttgg
     2521 tacactaccc acaaaccatt tattggcttg tgtgccattt acaactgtcc aattattggt
     2581 gccaaatgtt gctccggttt caaatcctcc atctccggtg gggcttatca ataccgtttg
     2641 tgctgaagtt ttattcaaaa aaataaaaac ggtgaagagt aaaaatagaa ttttgtgtgg
     2701 tttaattttc atttttaaaa tcataatagt gaagcaataa gtattattcg tgaaccaatt
     2761 ttcctttatc attcaaaaat agaaacctgt ttaaaacagg gtctttatat tttgcatcta
     2821 tgttttgtgt gcgaaaaaat tgcaataatt cttcgggctt aaaactttga atatgttcaa
     2881 caatcaaaat attattttga gcaatagtgt aactcaaaac cttaccctgc aaagtttcta
     2941 gcgcattaaa gatgtggaca tttgaattcg gtttcttttg tgccggaatc aaaaattcag
     3001 aagtcactat atgttcgcct gtgcgcgtat cggttttgat tattggttgt gcggcaatat
     3061 ttttttgtgc agcaacatac atttgcataa tacctgagca aacaataaaa agaaaatatt
     3121 ttttcatttc gatattgatt tgttaacaat aaaaggtgta aatatttata attatactag
     3181 caatcacaaa ccctctattt ttacagtgta atactactaa actaatagaa tatacgcaac
     3241 attcttttgc caaaaaaaat gaaatttttt ttagagttgt tttagtatgg atcttaaaaa
     3301 tttaaaaaga taatttatgg cagaatttga tctgcaaaaa aaggtgatca actaaaagaa
     3361 agttgtctat ttcaagtacc taattcctat ctaacttaag ccttgcaagt ttatcatcag
     3421 cattatacat ggtagttact ttcaatcggt cgcccgaaac ttttgttaaa gcccaggcaa
     3481 cagcatctgt tattttacgc ttgcccgata gtaattcata ctcttctaaa tccttatcgg
     3541 gagtggtttt cgagcctttt ttattgataa gagttgtaaa atattctttt atcgtttttc
     3601 ttttcgaatc ggcaacaggg gtattcaata ccaaagttcc tcttacaatt tcggcttgag
     3661 tttcaataat tgaattccca ttttggttgt tggtcgtatc tattttcaat ggattctcag
     3721 caatagattc ttctatagtg tcttttttag gtattgaaac aatatcattt gcttttaaat
     3781 tattctggcc tacagtatct tttacttgag caaggttttg ggttgcgttg taattacgaa
     3841 tttgcttaac ccttattttc gttgcatttg tgtttttatt aaatggcgca ttagactgtg
     3901 cttcaacatt cttttctatt gaatcaatga ttgcatttgt ttcggtagat atagtctccg
     3961 ctacctcctg ttgtttcctt tcggttttgc ttgctactga ttttgtattg gcattaactg
     4021 tgtcaatatt tttagtgtcg gccaaatttc ctttaccgtt attcaaaacc aaattaaggc
     4081 caaatgcaat gataaggcaa gcggctattc ccgataccac atattttaat gttgcatttg
     4141 gaaacaatac tgctcttttc aattttcctt taaatggata aatttctata tcacttgcat
     4201 tgagtttcga ttgttgatat aaagcaaggt cttttttgta ttgtgggttt tggtcaagaa
     4261 atatttccac ttccctccgt tcatcatagt tcaatattcc ttcgctgtag gcaattaaat
     4321 tttcatctat tttttcatgc tcaatatttg ttttgtaaag agtgctttta tccaaagcaa
     4381 ttttttcgtc aagcagcttt gcggatgcaa ataaaaattg ttcatcggca tattgagggt
     4441 tgttggctat aaaattgttc aatgcatttt gctctgcaat atttagcaag ccttccaatt
     4501 cggcaatcag ccaatcatca atattttcgt gagtaatatc gagtttgtaa agcgaagttt
     4561 tattttcaaa tcgctcttca ccggcaatta gtttactgtt ttttaatgct tctaattccg
     4621 atgcatcaat ttcaatgttt tctgcatcat gtagttttac gctatcaata tttgctaaaa
     4681 ttatttcctc ctgggccgat aatttcaaat ccattttagc atcaactttt aacaacaaat
     4741 ttttgttagg gaaatcgatt ggcaaatttt cgatggataa attttcaaag tgcgaaaaag
     4801 catttttaaa ttgtgggtga ttgtcgagaa ataaaagcaa ctgcaaaacc tcctcgggag
     4861 tgaggcggtt ttcgtgatag tctaaaaacc acgcttcgca attatgtata tttatttcgt
     4921 gtttcatttt tttatatcac atttcgcatg ctgccaataa actctttcaa agtttggcgt
     4981 gcgcgaaaaa tgtaaacttt tacctgcgat tcatttaact gagttatctc tccaatttcc
     5041 cgatagtcgt atccttcgta gtcgcgcaac attattacgg tgcgttgtat ttcgggcagg
     5101 cgcttaagtc cttcgtgcaa tatttctttc aatcccgtgt acgattcttc tttgtaatgt
     5161 ttattaaaat ccacttcggt cattggcgct ttatatttat tgcgccttat tttatttatc
     5221 atggtgtggt atgccgtggt aaataagtac gactttacct tttggggatt tacattgtcc
     5281 ttgttaatcc acattttttc ataagtatcc tgcactatat cctgcgcttc ttctttgtcg
     5341 cgtatattat gtagtataaa gcgatatatg ccatcgctat actgatctac acacttattg
     5401 tattcgttgg tggtcaaggt ggtgagtttt gttattaatg atacgttttg gccctggaaa
     5461 aagttacatc cggcagcaac tatttatatt gtttcttgta aatgccttat caaaatgcgc
     5521 ttagctattg gcaataatgt agattccata ggcaaagcca acgatgattc tatggcaaaa
     5581 gttgcaggat ttggcaattg tttgtggcta tttagtaatt ctatttttaa ttgctcaaaa
     5641 gtattggtta tcgaaacttc atatgaacat acataatgag tatgtatacc tctatagcgc
     5701 tcatcgtgtc gttcgaaaag cgttatttgg taattgtaaa tatgcgttgc tttttcacct
     5761 ccattgcgca aaaacagata tccttcgttt actcttatgg gtgtaaggcc cacaggagaa
     5821 ataattaatt tgtgctccac aaagtcataa attttgcggc cttcaaacat atatttttca
     5881 aattgcggta tcgaaaaatt tactatctgt tctatttcac tcataatgct atcgtcattc
     5941 ataatgcttt cgtgcatgag tttaaattcg gcagcatcaa tacctgtaag tgtttcggga
     6001 aagttgctgc gtaattttaa agtgttttcc tgcaacaatt ttaagttttt gtaatgctct
     6061 ataattgagc tcaggtgtgg aaacaattct gttgcactaa atttttcgtt cacatgttga
     6121 agatacccca gcaacatata ctttttgtac tcgaaatcta tatgactttc tgttatccaa
     6181 tttgaattta atgtatccat acttttaaaa tttattgcca gcgaaaatta ttatttctat
     6241 caatatgtat ctagaatgtt tttaatgttt ttaaatttta tttgttcata aaataaccca
     6301 acccatgtaa catgtgcaat gggtctgcct aattttacat caaatatatt tccacgtttt
     6361 ttaaaaaatt atttatcatg ttattgaatt cgaaaaataa aattgcgttt agccttatc
//
LOCUS       DAORQA010000090         6354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3135_length_6354_cov_12.907287, whole genome shotgun sequence.
ACCESSION   DAORQA010000090 DAORQA010000000
VERSION     DAORQA010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3135_length_6354_cov_12.907287"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..431
                     /locus_tag="PK736_05910"
     CDS             75..431
                     /locus_tag="PK736_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32960.1"
                     /translation="MKNLILVFLFFAMFIATHEANAQPCNLPILNMHTVYIFTTKTNH
                     TMEEVMGAKLNPMLFNFVFVKTEDKLLEIKKLATENKLQSTVFVFFSNGKAIRYCNVE
                     EFAKALENKQLLEIEN"
     gene            complement(704..4968)
                     /gene="rpoC"
                     /locus_tag="PK736_05915"
                     /pseudo
     CDS             complement(704..4968)
                     /gene="rpoC"
                     /locus_tag="PK736_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
     gene            complement(5029..>6354)
                     /gene="rpoB"
                     /locus_tag="PK736_05920"
     CDS             complement(5029..>6354)
                     /gene="rpoB"
                     /locus_tag="PK736_05920"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633299.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRC32961.1"
                     /translation="KDLDEHGMIRVGADVREGDILIGKITPKGETDPSPEEKLLRAIF
                     GDKAGDVKDASLKVPPSVVGVVIDKKLFTRNIKDKVSKGDEKGILAKLDEDQAKTLAL
                     HKSHLIEKLFTLVNGKTSQGVNNLLKEVLIPKGTKFTQKILMDLNLANINPQKWTTDK
                     ELNDKISQLLHNYTIRCQDIMGATKRKKYALTVGDELPAGIMQLAKVYIAKKRKLKVG
                     DKMAGRHGNKGIVAKIVRPEDMPFLEDGTPVDIVLNPLGVPSRMNIGQIYETVLAWAG
                     EKLGLKFATPIFDGATLDDINSYTEKAGIPKYGVTQLYDGLTGERFDQSATVGIIYML
                     KLGHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWAIEAYGAANILQELL
                     TVKSDDVVGRAKAYEAIVKGDNMQAPGVPESFNVLLHELRGLGLKITLD"
BASE COUNT         1806 a         1410 c         1084 g         2054 t
ORIGIN      
        1 tgcattatat aaatattttt tgtcaattcc accaactatt tttgagctca ttacctaaat
       61 aaataaataa aattatgaaa aatctcattt tggtatttct cttttttgca atgttcattg
      121 caacacacga agcaaacgca caaccatgca acctgcccat tttgaatatg cacaccgtgt
      181 atattttcac cacaaaaaca aaccacacaa tggaagaagt aatgggagca aaattaaatc
      241 ctatgctttt taattttgtg tttgtaaaaa cagaagacaa acttttagaa ataaaaaaat
      301 tagcaacaga aaataaattg cagtcaacag tgtttgtttt ttttagtaac ggtaaggcta
      361 tacgttactg caatgtggag gagtttgcaa aggctttgga gaataaacaa cttttagaaa
      421 tagaaaatta ggcattgctt ttcgcaaact cgaattatac tggaagctgg ataaaattat
      481 acattccaga aaaaggtaag gccttaggta ttttgcaatt ttgcctatgc ctttatgcat
      541 cttttgcata agtgaacaat tttgtctcca aaaaagtata aacaagcatg cccatacatt
      601 ttacagaacc tatgaagtgg aaattgctat actactcaat caaaaaaaaa tcccgcttca
      661 attttcatca aagcgggaaa tttcaaatta tcaattcggt taattattct tctttcgagg
      721 ccattaacaa atcgtactct tcttgcgaac caactaccag tttatcgtac tcacgcattc
      781 cggttcctgc aggtataagg tggcctacaa ttacgttttc tttcaatcct accagattat
      841 ctatacgtcc ggcaattgcc gattcattaa gaactttagt ggtttcctgg aacgaagctg
      901 ccgatatcca actcttagta tgtaaagagg cctgagtaat tccttgcaat aatgggcgtg
      961 aagtagccga tacagcctgg cgtgcctcta ccggtgtttt atcgctgcgt ttaagcattg
     1021 aattttcatc acggagttta cgcacactta ctatttgccc cgctttaaat tgtgttgaat
     1081 cgcctggctc aagtattacc attttatcaa aaatttcatt attctcttca ataaaatcga
     1141 gacggtttgc agcttccctt tcgaggaact tagtatcgcc cggatcctca atctcaacct
     1201 tacgcatcat ttgacgtact attacttcaa agtgtttatc gttaattttc acaccctgca
     1261 aacgatatac ttcctgcacc tcatttacaa gatactcctg cactgcattt gggcctttaa
     1321 tattaaggat atcgccagga gaaattacgc catcactgag cggtaagcct gcgcgaataa
     1381 agtcgttatc ctgaacaaga atatgtttgg aaagagatac aagatatttc tttacatcgc
     1441 catcttttga tgttactaca atttcacggt taccacgctt gattccacca tatgtaacaa
     1501 gaccatcaat ttcggaaact acagctgggt tactcgggtt acgtgcttca aataattcgg
     1561 ttacacgtgg taaaccacca gtgatatccg aagtctttcc ggcattacgc ggaagtttta
     1621 ccaggatatt tcctgcagtt actttatcgc ctttatcaac aacaatatgc gcacctacag
     1681 gtatgttgta tgattttaat accgtcttat ctttagcaag aatttttact accgggttac
     1741 gtgctttatc gcgtgtatcg ataattactt tctccttaaa tcctgtttgc tcatcctgct
     1801 cctcgcggaa ggttataccc tcatcaatat gttcaaagtc aattgttccg gtagcttcgg
     1861 taatgattac agcattgtaa gggtcaaact tacatatttc ctgacctttg gtaacctgct
     1921 gaccttcgtc tacaaacaca tgggcaccat aaggtatatt gtttgtggca agaatagcac
     1981 gtgtttttgt gtcaatgatt ctcatctcac ccgaacgacc tacaacaaca aacaatttct
     2041 ttccatctat ttcgaatggt agcgtacgaa gttcatcgaa ctcaattata ccggtatatt
     2101 ttgccagaat gttggtttca gcagccaact tacttgcggc accaccggca tggaatgtac
     2161 gaagggtaag ctgtgtacca ggctcaccaa tggactgtgc tgcaattact ccaactgctt
     2221 cgccacgttg taccattcgg ccagtactta aattacgacc gtaacattta ccgcatacgc
     2281 cctgcttact ttcacatgtc aataccgatc gtatttcaac gctttctatt ggagaatttt
     2341 caattaattc tgcttttgct tcaataattt cttgtccggc agaaacaagt aattcaccgg
     2401 taagaggatg ataaatatcg tgcaaagaaa cccggcctag tatcctgtca tataaagact
     2461 caacaatctc ttcgttattt tttaatgctg tttcagccaa tccgcgcaat gtgccacaat
     2521 cttcctgatt gataataaca tcttgtgcca catctactaa cctacgcgtt aagtaacccg
     2581 catcggctgt tttaagggct gtatcggcca aacctttacg ggcaccatgg gtagaaataa
     2641 aatactcgag gattgaaagt ccttccttaa aattggaaag aattggattc tcgataatct
     2701 cgcctcccga tgcgccagat ttttgtggtt tcgccatcaa tcccctcata ccactcaact
     2761 gacgaatctg atctttacta ccacgggctc cagagtcaag catcatccat accgcattga
     2821 agccttgctt atcggcagca atttttttca tcaggttttc tgtgatgcgt gaattggtac
     2881 gtgaccaaat atcaattatc tgattgtagc gctcgttgtc ggtaataaaa ccattacgat
     2941 agttatcaat tacttccaac acttcatctt cagctttgcc tatcaatttt tccttgtcta
     3001 cgtcaatgtc gatatcacca atattgaatg acaatccacc tttgaaggct gaacggaaac
     3061 caaggttttt aatatcatcg aggaattgtg cagtacctgc tattccaact actttcaata
     3121 cgcgttggat aatatcacgt aatgatttct tcgtaagcaa ctcatcgata tatccaactc
     3181 catcgggcac tacctgattg aacaatgcac gaccaacata agtatcaatt attttcgaaa
     3241 ccttttgtcc gttttcggtt agggttattc gaatttttat ggcagcatgc aaatctacca
     3301 tgccttcatt gtaagcaata tttaattctt cgtaagaata aaacgtaagt ccttcacctt
     3361 ttacaggacg ctcgggagtg cccttacggc ttttggtaat atagtacaat cccaaaacca
     3421 tgtcctgcga tggtaccgtt ataggtgcgc catttgcagg gttaaggata ttgtgcgatg
     3481 caagcataag catttgtgct tcaatgattg cggcatttcc aagcggaacg tgaacggcca
     3541 tctggtcacc gtcaaagtcg gcattaaagg ctgtacaaac caatggatgt aactgaatgg
     3601 cttttccttc aatcattcga ggttgaaacg cttgtatacc aagcctgtga agtgtagggg
     3661 cacgattcaa cattacagga tggcctttca acacattttc gagaatatcc caaataatag
     3721 gttctttttt gtcgactatt ttctttgcag attttaccgt tttaactatt ccacgctcta
     3781 tcatttttcg aatgataaac ggtttgaata attccgatgc catatctttt ggcaatccac
     3841 actcatgcaa tttcatttca ggccctacaa caattaccga acgtgcagaa taatcaacac
     3901 gttttcccaa taaattttga cggaatcgtc cttgcttacc tttcaatgag tcggaaagtg
     3961 atttcaatgc acggttgctt tcggttttta cagcgttaac tttacgcgag ttatcgaata
     4021 aagaatccac cgattcctga agcatacgtt tttcgttacg taagattaca tcaggagctt
     4081 ttatttccat caatcgcttt aatcgattgt tgcgtataat caccctacga tacaaatcat
     4141 ttaagtctga tgtggcaaaa cgaccgccat ccaatggaac aagtggacgc aattctggtg
     4201 gaattacagg taccactttc acaatcatcc actcggggcg attgataata tgttcattgg
     4261 cactacggaa tgactcaaca accgaaaggc gctttaatgc ttcattctta cgttgctgcg
     4321 atgtctctgt agctgcctga ttacgcaagc gatatgacaa ttcatctaaa tcaagacgtg
     4381 ccaataaatc acttagtgat tctgcaccca ttttagcaat aaacttattt gggtcagaat
     4441 cgtcaagcat ttgattttcc ttcggcaaag tttcgagaat atttacatat tcatcttcgg
     4501 taagaaaatc aagatagttg attccatccg gctcttttat tcccgcgtta attactacat
     4561 aacgctcata ataaatgatt aaatctaatt ttttggttgg caaaccaaga aggtaaccaa
     4621 ttttatttgg caacgaaaat accaaatatg agcaaccgga actaccagtt gaatgtgccc
     4681 catgcgctct ctccgtactt tcttttcggt aacctctacc ccacaacggt cgcaaacaat
     4741 gcccttgtaa cgtatgcgtt tgtatttacc gcaatggcat tcccagtctt ttaccgggcc
     4801 aaaaatacgc tcacaaaaca atccatcgcg ttccggttta taggtacgat aatttattgt
     4861 ttcgggcttt aatacctcgc ctgccgattg ttctaaaata tcttcgggcg atgataaact
     4921 gatggtaatt cgtgaaaaat tactcttaac ttttaattcc tttttcaatg ttcttttgtt
     4981 tatttatgga gaattaaata tcttatttat taaagtttag gaacaggatt aatcaagtgt
     5041 tatttttaat cctaatccac gcaattcgtg caacaataca ttgaacgatt cgggtacgcc
     5101 cggtgcctgc atgttatcac cttttacaat agcttcgtat gcttttgcac ggccaacaac
     5161 atcatccgat ttcacagtaa gtaactcctg aagaatattg gcagctccgt atgcttctat
     5221 ggcccaaact tccatctctc caaaacgctg ccctccaaac tgagccttac cacccaaagg
     5281 ttgttgcgta ataagagaat atggtcctat tgaacgggca tgcatcttat catccaccat
     5341 atgtccaagt ttcaacatgt atattattcc cactgtagct gattggtcga aacgctctcc
     5401 tgttaatcca tcataaagct gtgttacacc atattttggt attcctgctt tttcagtata
     5461 actattgata tcatccaaag ttgctccatc aaaaattgga gtggcaaact taagcccaag
     5521 tttctcacct gcccatgcca aaacggtttc gtaaatctgt cctatattca tacgtgaagg
     5581 tactccaagt ggattcaaaa cgatatctac cggggttcca tcttcaagga aaggcatatc
     5641 ttcgggacgt acaatctttg caacaatacc tttgtttcca tgacgaccgg ccattttatc
     5701 accaactttt agtttacgtt ttttagcaat atatactttt gccaactgca ttattcctgc
     5761 tggtaattca tctcctacag taagggcata tttcttacgc ttggtagctc ccattatatc
     5821 ctgacaacgg atagtataat tgtgaagcag ctgactaatt ttatcgttta attccttatc
     5881 ggttgtccac ttttgaggat tgatgtttgc aagatttaaa tccatcaata tcttttgcgt
     5941 gaactttgtt ccttttggga ttaaaacctc tttaagcaaa ttgtttacac cttgcgatgt
     6001 ttttccattt acaagggtaa aaagtttctc tatcaaatgg cttttatgca acgccaatgt
     6061 tttagcctgg tcttcgtcaa gttttgcaag aattcctttt tcatctccct tgcttacttt
     6121 atccttaatg ttacgtgtaa acaatttttt atcgattacc acacctacta ctgaaggtgg
     6181 aacctttaac gaagcatctt ttacgtcacc ggctttgtca ccaaagatag cgcgcaacaa
     6241 tttttcttct ggcgaaggat cagtttctcc ttttggagta atttttccaa ttaggatatc
     6301 accttcacgc acatctgctc ctactcttat cattccatgc tcatcgaggt cttt
//
LOCUS       DAORQA010000091         6328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3143_length_6328_cov_6.954886, whole genome shotgun sequence.
ACCESSION   DAORQA010000091 DAORQA010000000
VERSION     DAORQA010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3143_length_6328_cov_6.954886"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PK736_05925"
     CDS             <1..186
                     /locus_tag="PK736_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRC32962.1"
                     /translation="SKDDSRNTEPFGIILMGELFQNNHHEHPMSANFAQKWWEFDLTY
                     PVLKMLSWLHIIKLKQA"
     gene            247..1215
                     /locus_tag="PK736_05930"
     CDS             247..1215
                     /locus_tag="PK736_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HRC32963.1"
                     /translation="MGPISQFMEKNYLHFNSAAMMDASKAYVTHMNDGGKMMITLAGA
                     MSTAELGISLAEMIRQDKVSIISCTGANLEEDLMNLVAHKHYKRVPNYRDLTPQDEWD
                     LLENHYNRVTDTCIPEEEAFRRLQKHIYGVWKAAEDKGERYLPHEYMYKILLSGELEQ
                     YYEIDPKNSWMLAAAQKNLPIVVPGWEDSTMGNIFASYVIKNELKASTMKSGIEYMVW
                     LSDWYRKNSGGKGVGFFQIGGGIAGDFPICVVPMMYQDLEWHDVPFWSYFCQISDSTT
                     SFGSYSGAVPNEKITWGKLGIDTPKFIVESDASIVAPLMFAWILGM"
     gene            1396..2307
                     /locus_tag="PK736_05935"
     CDS             1396..2307
                     /locus_tag="PK736_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRC32964.1"
                     /translation="MLKKSLSKLVMLIALVFGQYAVNAQCQTPSNLGATINGPNMLLT
                     WNAVGSATSYRVNIQNAPGNNVFLDVEFNSATNSFMAMNLTPGSNYKFKVRTNCGGNN
                     SAWSGYFFFTANGAACNPLQITGVTTINNVNHTTTINWNAVPGVSSYRVRIENGQNNP
                     VPFAFVRNPITNTLTVSVLAPATNYKVKVRANCNGNLGPWSVWKQFTTGPARIGNNTV
                     AENITIDAYPNPANDLLQFEISGKINSEKIMAQLFSLTGMKTAEFNFNVSEDELSINT
                     QNIPAGIYMLKINDGTNAITKRISIVH"
     gene            2429..3760
                     /gene="chrA"
                     /locus_tag="PK736_05940"
     CDS             2429..3760
                     /gene="chrA"
                     /locus_tag="PK736_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HRC32965.1"
                     /translation="MEKEIRAKPSTKDALLFWLKLGFISFGGPAGQIAIVHEYLVENK
                     KWISPSRYLHALNYCMLLPGPEALQLVIYTAWLLFGTWMAIIAGLLFIIPSIILLLVL
                     SFLYCQYGHLPLVASMLLFVKPAVVAMVIGALIKIGKKSLTSATYIAITIISFVLIYF
                     FKIDFALLVIVTLVLGALYSWQNRKQMSDTKNAKSDTTESEYFIHQHSAFHYEWKNVW
                     QHIAKISSGFIFICFLPIGVIYFFSSHQSFWYTLSGFFTKAAFVTFGGAYAVLPYVGQ
                     VAVEKYNWLTHAQMMDGLALGETTPGPLIIVLQFVGYMAGYNAFHQNIFAGIIAALLT
                     SFYTFVPSFFMVLAGGPVIEKTKDNPLVKNTLKFVNALVVGVLANLCLSLILNLWNLK
                     SIHFSAQAIFTLLWLLVSILVFQKLKMNVIKWIALSLICGVVWFFIAPFIN"
     gene            complement(3863..4234)
                     /locus_tag="PK736_05945"
     CDS             complement(3863..4234)
                     /locus_tag="PK736_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HRC32966.1"
                     /translation="MSGEQHGGYTTGDIHNQFVEEHYDPQNVKTIWRTFWILLALTIL
                     EVGIALTSLSKVMLMYTFIILTVVKAYFIIAYFMHLKHEKKALQYVIILPFLLIGYLI
                     FISITEGVYIHGMDHGFFNTK"
     gene            complement(4288..5100)
                     /locus_tag="PK736_05950"
     CDS             complement(4288..5100)
                     /locus_tag="PK736_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRC32967.1"
                     /translation="MSETLTIEKSKRSPWGGGISPFNVSWGKMYMWFFLVSDAFTFSS
                     LLIAYGTLRHRFTEIWPLASEVFTHFPFVHGHVQLAYVALMTFILIMSSVTMVLAVDA
                     GHRRDNKSVAVWMALTIVGGFMFLGSQAWEWYHFIEGSEQGSYVLADGRFARTDDYEP
                     GTIFFRGGEKVEGAEAKALIASATKHTIGANLVENEYGPTPLFANFFFFITGFHGFHV
                     FSGVMINLIIFFNVIDGIYKKRGHYEQVEKAGLYWHFVDLVWVFVFTFFYLV"
     gene            complement(5217..6317)
                     /locus_tag="PK736_05955"
     CDS             complement(5217..6317)
                     /locus_tag="PK736_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HRC32968.1"
                     /translation="MKIAIMGTRGIPNNYGGLEQFAERVSELLVKKGHEVIVYNASHH
                     PYDKSEFHGVKIIKKYNPEKKIGSGGNFIYDLLSYYDASKRNCDAILVCGYTTMAFAY
                     LLVKPKSKVVTNIDGMEWWRSKHNNIVKKFTHWTEKLAIKSSDAIISDNIGIQDYVME
                     DYKKHSYFIAYAADLFDNNNPAILKEWNLTPYDYNAMICRLEPENNIEIILDGVAMSK
                     STMKTYVCASYEHKYGYYLKDKYKDCDKIIFKGWVAKQEILHQLRNYSNLYFHGHSVG
                     GTNPSLLEAMAGKAFIAAHDNVFNRHVTGDDALYFKTPADVAHMIDNYDGVKQSRERF
                     THNNLEKIKHIYNWQNIADQYEAMFKEVTGKN"
BASE COUNT         1982 a         1150 c         1152 g         2044 t
ORIGIN      
        1 agcaaagatg atagtcgcaa tacggagcca tttggaataa tattgatggg cgaattgttt
       61 caaaacaatc atcatgaaca tccaatgtcg gctaacttcg cacaaaaatg gtgggaattt
      121 gatttaactt atcctgttct taaaatgctg agttggttgc acatcattaa actcaaacag
      181 gcataaactt ttttgttcga tttcaatatc aattttttaa ttctttttaa aataattcta
      241 tataatatgg gacctatatc tcaatttatg gagaaaaatt atctccattt taattctgcc
      301 gctatgatgg atgcatcaaa agcatacgtt acacatatga acgatggcgg aaaaatgatg
      361 ataacacttg ccggtgcaat gagtacggca gagttgggaa tatcgcttgc tgaaatgata
      421 aggcaagata aagtttctat catttcgtgc acgggtgcaa acctcgaaga agacctgatg
      481 aatcttgtag cacacaagca ttataagcgt gttccaaatt atcgtgatct tactcctcag
      541 gatgaatggg atttgctcga aaatcattac aaccgtgtta ccgacacctg catacccgaa
      601 gaagaggcat ttcgcagatt acaaaaacat atttatggtg tgtggaaagc tgccgaagac
      661 aaaggcgaac gttatttgcc tcatgagtat atgtacaaaa tattgctgag tggcgagttg
      721 gagcaatact atgaaataga tccgaaaaat tcgtggatgc ttgctgccgc acaaaagaat
      781 ttacccattg tagttcccgg ttgggaagat agtacaatgg gaaatatatt tgcttcttat
      841 gttataaaaa atgaattgaa agcttctact atgaaaagtg gaatagagta catggtatgg
      901 ttgagcgatt ggtatcgtaa aaacagtggt ggcaaaggag taggattttt tcaaattggc
      961 ggaggtattg caggcgattt tcctatatgt gttgtgccta tgatgtatca ggatttggag
     1021 tggcacgatg ttcctttttg gagttacttt tgtcagataa gtgattctac cacatcgttt
     1081 ggttcgtaca gtggtgcagt acccaacgaa aaaatcacct ggggtaaact tggaatcgac
     1141 acaccgaaat ttatagttga aagtgatgct tctattgtag caccgttaat gtttgcctgg
     1201 atactgggta tgtaacggta gctttatacg tatactgata aattattata caggctctga
     1261 aaaaatttca gagctttttt tttggctctc agatgtaaaa tcttccgctt gagcattttt
     1321 aatttttaac ctaaacaaat gctcaattta tttggtgcat aattctctta ttaataattt
     1381 taaaaattaa atgttatgtt aaaaaaatca ctttcaaaat tagtaatgct catagcactt
     1441 gtctttggac aatatgctgt gaatgcacaa tgtcaaacgc cttctaactt aggtgcaaca
     1501 attaatggcc ccaacatgtt actcacttgg aatgccgtgg gcagtgcaac ttcttataga
     1561 gtaaacattc aaaatgcacc cggcaacaat gtgtttcttg atgttgaatt taacagtgcc
     1621 acaaatagtt ttatggcaat gaatcttact ccgggttcga attataaatt taaagtacgc
     1681 actaattgcg gtggaaacaa tagcgcgtgg tcgggatact ttttctttac tgcaaatggt
     1741 gcagcatgca atcctcttca aattacagga gtaacaacta ttaataacgt gaaccatacc
     1801 acaaccataa actggaatgc tgtgcctggt gtatcgtcat accgggtgcg aatcgaaaat
     1861 ggtcaaaaca atcctgttcc gtttgctttt gtgcgcaacc ccattaccaa tacgctaact
     1921 gtttcggtgc tggctcctgc tacaaattat aaagtaaaag tgcgcgcaaa ttgtaatggc
     1981 aatttaggtc catggtcagt gtggaaacaa tttacaaccg gtccggcacg tattggtaac
     2041 aatactgttg cagaaaacat aactatagat gcgtatccaa atccggcaaa cgacttgttg
     2101 cagtttgaaa tttcaggaaa aattaacagt gaaaaaataa tggcgcaatt attcagcttg
     2161 accgggatga aaactgcaga atttaatttc aatgtttcag aagatgagtt atcaatcaac
     2221 acacaaaaca ttcccgcagg tatttatatg cttaaaataa atgacggcac aaatgcaatt
     2281 accaaaagaa taagcattgt ccattaaaat tattacgaat aacaaaaaat ccggaacgat
     2341 gtttcggatt tttttttgcg taaacattac cactcaaaat tatatatgaa ctttctttta
     2401 cctatttttt gaaaattagg attaatctat ggaaaaagaa attcgtgcaa aaccctcaac
     2461 aaaagatgct ttactttttt ggctgaagct tgggttcata agttttggcg ggcctgccgg
     2521 gcaaatagct attgtacacg aatatcttgt agaaaacaaa aaatggattt cgccttcgcg
     2581 ttatttgcat gcacttaact attgcatgtt gctacccggc cccgaagcat tgcaacttgt
     2641 aatttatact gcatggttat tatttggaac ctggatggcc attattgcgg gtttgctttt
     2701 tattatccct tccataattt tattgcttgt attaagcttt ctttattgtc agtatggtca
     2761 tttgcctttg gtagcttcta tgttgctatt tgtaaaacct gcagtagtgg ccatggttat
     2821 aggggcatta ataaaaattg gcaaaaaatc attgacttca gcaacttata tagctataac
     2881 aataatttca tttgtgttga tctacttttt taaaatagat tttgcacttc ttgttattgt
     2941 aacattagtt ttaggtgcat tatacagttg gcaaaatcgc aaacaaatgt ccgatacaaa
     3001 aaatgcgaaa tcagacacca ccgaatcgga atattttatt catcagcaca gcgcattcca
     3061 ctacgaatgg aaaaatgtgt ggcagcatat tgcaaaaatc agtagtgggt ttatatttat
     3121 ttgttttttg cccattggcg tcatatactt tttctcatcg catcaatcgt tttggtatac
     3181 tctttcagga tttttcacca aagctgcatt tgtaactttt ggtggggctt atgccgtttt
     3241 accttatgtg ggtcaggtag cagtagaaaa atataattgg ctcacacatg cccaaatgat
     3301 ggatgggctt gcattgggcg aaaccacacc ggggccgctt attattgttc tgcaatttgt
     3361 aggatatatg gcggggtata atgccttcca tcaaaatata tttgctggta ttattgctgc
     3421 attgcttacc tcattttaca cttttgttcc ttcgtttttt atggtgttgg caggcggtcc
     3481 ggttatagaa aaaacaaaag acaaccctct ggtaaaaaac acattgaagt ttgtaaatgc
     3541 tttggtggta ggcgttttgg caaatttatg cttgtccctt attctcaatt tatggaatct
     3601 caaatcaatt catttttcgg cacaagcaat tttcacattg ctatggctgt tggtatcgat
     3661 tttagtattt caaaaactaa aaatgaatgt tatcaaatgg atagcattaa gtcttatttg
     3721 cggggttgtt tggtttttta ttgcaccttt tatcaattaa aataaaagcc tttgtattcc
     3781 ctatttgctt atgaataatg cagaaacaaa aaaagcacga acacatttaa ctgtattcgt
     3841 gctttactat gttttagaaa gattattttg tattaaaaaa accgtgatcc ataccgtgaa
     3901 tataaacgcc ttcggtaatt gaaataaaaa ttaaataacc tattaacaga aatggaagaa
     3961 taattacata ctgaagtgcc tttttctcat gctttaaatg cataaaataa gcaataatga
     4021 aatacgcttt tactacagtt aatatgatga aagtatacat caacataact tttgataatg
     4081 aagttaatgc tataccaacc tccaaaatag tgagtgccag taagatccaa aatgtacgcc
     4141 aaatagtttt aacattttgt ggatcataat gttcttcgac aaattgatta tgtatatcgc
     4201 ctgttgtgta acctccgtgt tgttcgcccg acatatttat tttgattttt aagattaata
     4261 ttgaattgtt gtgaaatgaa agatgaatta taccaaatag aagaatgtaa atacaaatac
     4321 ccaaaccaaa tctacaaagt gccagtacaa tccagctttc tctacttgtt cataatggcc
     4381 acgttttttg taaatgccat ctatcacatt aaaaaatatg ataagattaa tcattactcc
     4441 actgaatacg tgaaaaccgt gaaaaccggt aatgaagaaa aagaaattgg caaataatgg
     4501 cgttgggcca tattcatttt caacaaggtt agcacctatg gtatgctttg ttgccgaagc
     4561 aatcaatgct ttggcttctg caccttcaac cttttcgcca ccgcgaaaga aaatagttcc
     4621 tggttcataa tcatcggtgc gtgcaaaacg gccatcggct aatacataag aaccctgctc
     4681 gctgccttca ataaaatgat accattccca tgcctgtgag ccaaggaaca tgaaacctcc
     4741 aacaattgta agtgccatcc atacggctac acttttatta tcgcgtctgt gtccggcatc
     4801 tacagccaat accatggtta cagagctcat gatgaggata aaagtcatta aggctacgta
     4861 tgccagttgt acatgaccat gaacgaatgg gaagtgagta aacacctcac ttgcaagcgg
     4921 ccatatctct gtaaatctat ggcgcaaagt gccatatgca attaataaag aggagaaagt
     4981 gaatgcatcg gatacgagaa aaaaccacat atacattttc ccccaactca cattaaatgg
     5041 cgaaatgccg cctccccaag gtgagcgctt tgatttttcg attgtcaaag tttctgacat
     5101 aaattattga taataaatga tttacagttt aaaattcact aaattacagg aggtaggcct
     5161 gctttcgggc aacgaaaata caaaaatctt tattcaaact aattttttaa aaaagtttaa
     5221 ttttttcctg tcacttcctt gaacatcgct tcatattggt cggcaatatt ttgccagtta
     5281 tatatgtgtt ttattttttc taaattatta tgtgtaaacc tttcgcgact ttgctttaca
     5341 ccgtcataat tgtcaatcat gtgtgcaaca tcggccgggg ttttaaaata taaagcatca
     5401 tcgccagtaa catggcggtt aaaaacatta tcgtgggccg caataaatgc tttgcccgcc
     5461 atcgcctcta acagcgaagg attggttcca cctaccgaat ggccatgaaa gtacaaattg
     5521 gaatagttgc gcagctgatg cagtatttcc tgttttgcta cccatccctt aaaaatgatt
     5581 ttatcgcaat ctttatattt atctttcaag taataaccat atttatgctc gtatgaagca
     5641 catacatatg ttttcattgt tgactttgac atggcaaccc catcaagtat tatttcaatg
     5701 ttattttcag gctccaaacg gcaaatcatt gcattgtaat cataaggagt caaattccat
     5761 tcttttaaaa ttgcaggatt attgttgtcg aataaatcgg cagcataggc gataaagtaa
     5821 gaatgctttt tgtaatcctc catcacataa tcttgtattc ctatattgtc cgatataata
     5881 gcatcactgc ttttgatggc aagtttttcg gtccagtggg taaatttttt cacaatatta
     5941 ttgtgcttgc ttcgccacca ctccatgcca tcaatgtttg taaccacttt cgattttggt
     6001 ttaaccagca aataagcaaa ggccattgtg gtgtatccgc aaacaagtat agcatcgcaa
     6061 tttcgtttcg atgcatcgta atagcttaac aaatcataaa taaaatttcc tcccgaacca
     6121 atcttttttt cggggttata cttctttatt attttcactc cgtgaaattc gcttttatca
     6181 taagggtgat gcgatgcatt atatacaatc acttcatggc ctttttttac aagcaattct
     6241 gatacgcgtt cggcaaactg ctccaaacct ccatagttgt ttggaatacc gcgagtaccc
     6301 attatagcaa ttttcataaa tcgttaga
//
LOCUS       DAORQA010000092         6239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3193_length_6239_cov_24.126617, whole genome shotgun sequence.
ACCESSION   DAORQA010000092 DAORQA010000000
VERSION     DAORQA010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3193_length_6239_cov_24.126617"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..439
                     /locus_tag="PK736_05960"
     CDS             203..439
                     /locus_tag="PK736_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32969.1"
                     /translation="MRIAIKNTTIDKYFGFLTKLDNISKKRLIVKLTESIEVKEKKQF
                     DLKSLYGAWEDTRTSEEIIKDIRNSRIEKKSIDL"
     gene            893..2626
                     /gene="acsA"
                     /locus_tag="PK736_05965"
     CDS             893..2626
                     /gene="acsA"
                     /locus_tag="PK736_05965"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRC32970.1"
                     /translation="MKENITIIKHNLVDYESVKADFLWENIAKELVGLPGNKELNIAL
                     EAVDRHADGQLKNTVAIRFIRKDRSFRELTYSGLKIQTAKFANVLQQLGIKKGERVFS
                     LAGRVPELYITALGTLKYTAVFCPLFSVFGPEPILQRMSKGEAAVLVTTSAMYEKKVK
                     QLQAQLPFLRYILLTDIADHVSKNVLSFSKLMEQAIENFTIPPTNPEDAALLHFTSGT
                     TGMPKGVLHVHQAVLTHYITGKYVLDFHPGDVFWCTADPGWVTGTSYGIISPLVNGVT
                     SIVDEEEFDATRWYSILEEHKVNIWYTAPTAIRRLMRMNINPLQEYKLDNLRLILSVG
                     EPLHAEGVIWGKEKFGIPILDNWWQTETGGIMIANYASMEVKPGSMGKPLPGVEVAIA
                     TIESGKLKFITEPNIEGHLVLKKGFPSLFRTYLHEEERYNKCFFGDWYLSGDLAKRDK
                     DGYFWFIGRADDIIKTSGHMVGPFEVESTLMRHSFVAEAAVIGKPDPSIGELVKAFVV
                     LKPGIEQNNVVKMDIMGFARKEMGPAIAPKEIEFIESIPKTRSGKVLRRLLKARELGL
                     PEGDLSTLETG"
     gene            2661..3623
                     /gene="pdhA"
                     /locus_tag="PK736_05970"
     CDS             2661..3623
                     /gene="pdhA"
                     /locus_tag="PK736_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRC32971.1"
                     /translation="MNAEKGRQLLYQMLLIRRFEEKSAEQYTKAKIRGFLHLYIGEEA
                     VAVGVIQALTDEDNILSTYREHGHALARGLSPQSIMAEMYGKQEGCSMGRGGSMHLFD
                     ASKRFYGGNAIVAGHLPLAVGMALASKKQNKKNITCCFFGEGAAAEGEFHESMNLAAL
                     WNVPVLFICENNQYAMGTALKYSHSVLEMEKKGMAYGIETVVVDGMNLNAVINEADKA
                     VAKIKETGRPYFLVCNTYRFRAHSMFDAELYRDKKEVEEWKQRDPIADFTNYVLEQQF
                     LTEQNILEIENRVNIEVEEAVVFAENGTREKVEDLTKFVYSKQK"
     gene            3620..4051
                     /locus_tag="PK736_05975"
     CDS             3620..4051
                     /locus_tag="PK736_05975"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HRC32972.1"
                     /translation="MKTLEKQDINSWACPKCGREFLRKGQTHSCKTFPLEQHFSGKTK
                     WRKLFNKLSEAISTRIGNFKIDSIECCIHLVHQSTFTAVKVMKDRLRVDFSLDYELSN
                     ARIGKTVKMSKNRFLFYVDITKEDDIDTELIQWLTEAYFLK"
     gene            4115..5101
                     /locus_tag="PK736_05980"
     CDS             4115..5101
                     /locus_tag="PK736_05980"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRC32973.1"
                     /translation="MNNTPITYREALKQGIREALQNDERVFLMGEDVGRYGGAFAVSK
                     GLLHEFGEDRIMDVPLCESGFVGAGIGAAMVGMKPIVEVMTVNFSLLAMDQIVNNAAT
                     ILHMSGGQINVPVVIRMGCGIGRQLAAQHSHSWEPLIAHIPGLIALSAGTHEDARYML
                     AEALKSPDPVIIFEYTSMLNTEREIPENITHVDISKAKVRREGKDVSIITYGTGVYKC
                     LDAANALAALGIDAEVVDLRVLRPLDEETILASVSKTHRALIVEDAWKSVSISAEISA
                     RIMEKIFYELDAPVMRICGVEVPIPYPIHLEDASVPQKNDIINAVKKMLNHD"
     gene            5094..>6239
                     /locus_tag="PK736_05985"
     CDS             5094..>6239
                     /locus_tag="PK736_05985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRC32974.1"
                     /translation="MIEFVMPSLGADMEDGTLAQWRKKEGDTVKRGDIIADVETQKGL
                     IEIELFDEGVIEKLLIEPGTKVPVGTPLALIKPSKQKAETIITTAAKEEEIHPITEKE
                     ITQVPHESFDKKFKISPLAKRIAAENNIDISTLMGSGENGVITKEDVENAIAQKQKSI
                     ATQETSGAATEDKTKSASNSIRAAVAAAMSKSNREIPHYYLEKTIDMTKALAWMNESN
                     KERPVKQRLLPAALLIKAVALSLREVPELNATWENGLQIKSDINIGFVVSLRGGGIIV
                     PAIHSANVKTVDEIMTTLNDIIPRAKALKLRSSELSDSTVIITSIGEGGADSVYGIIY
                     PPQVAIIGFGNIAEQPFAVNGMLGVHPLVRATLAADHRAADGLTGSRF"
BASE COUNT         2096 a          995 c         1398 g         1750 t
ORIGIN      
        1 agccctcagc tatgtgatgc atatttattt ttttgctaag gtatgtcaac ggtgtcgttg
       61 taatttttat ttttaccaaa tgttgttaat gtgcaactgt tgggtaatgg ttttttcatg
      121 ggctgacctt ggtggcaatg cgcaaaaatg atagaaagaa attggacata ttttttagta
      181 tctttgtgaa aatgaagagt ttatgagaat agcaataaaa aatactacta tcgacaaata
      241 ttttggtttt ttgaccaaat tagacaacat ttcaaagaaa cgactgattg ttaaattgac
      301 agagtctatt gaggttaaag agaagaaaca gtttgacttg aagagcttgt atggtgcatg
      361 ggaagacaca agaacttcag aagaaataat aaaggatata agaaattcac gaatcgaaaa
      421 gaaatcaatt gacctgtaat gaaatatttg cttgacttaa atatgtgcat tcatttttta
      481 agaggaaaat ataacataat tgaaaaattg aatgaagtag gcttagacaa ttgtgcagtt
      541 tgagaaatta cattggcgga gttagttttt ggagcagaaa aaagtgacaa tcctaaaaag
      601 aaccataaac taattgaaaa atttattgga cactaagtgt tttacccata tttgacgcaa
      661 ttcagacata tggtaaagaa aaagcactac tacaaagcga aggaaaaatg attagtgatt
      721 ttgacttact tgatgttagt cattttccgt tatttcatta tgaaattaat ttacaccgta
      781 atattcctca tttgatgaaa aaagtgcatc gaagattatc agctgaactt taatgcaatt
      841 gaatcaaaaa gtaattagag ggcgaaatag ttgagagtta aaattataag ctatgaaaga
      901 gaatataacg attataaaac acaatttagt tgactatgaa tcagtaaagg ctgatttttt
      961 atgggaaaat attgctaagg aattagttgg attacccggt aataaagaat tgaacatcgc
     1021 acttgaagcc gttgatcgcc atgccgatgg tcagctgaaa aatactgtag caattcgttt
     1081 tatacgaaag gatagaagtt ttagggagtt gacatattcc gggttgaaaa tacaaaccgc
     1141 aaagtttgcc aatgtattac aacagttagg aattaaaaaa ggagaacgtg ttttttcatt
     1201 ggctggaagg gttcccgaat tgtatatcac agcacttggc actttgaaat atactgcggt
     1261 tttctgccct ttattttctg tttttggacc tgagcccatt ttacaaagaa tgagtaaagg
     1321 ggaggcagcc gtgttggtaa caacttccgc tatgtatgaa aagaaggtaa aacaattgca
     1381 ggcgcagctt ccgtttctta ggtatattct gctaaccgat atagccgatc atgtttcaaa
     1441 aaatgtgttg tccttttcca aacttatgga acaagccatt gagaatttta ctattcctcc
     1501 aaccaatccc gaagatgccg ccttgcttca tttcaccagt ggtacaacag gaatgcccaa
     1561 aggcgtttta catgttcacc aagctgtact tacccattat ataactggga aatatgttct
     1621 tgattttcac ccgggagatg tgttttggtg cactgccgat cccggttggg taacaggaac
     1681 ttcatatggt ataatatcac ctttggtaaa tggagttaca agcattgttg atgaagaaga
     1741 atttgatgca acaaggtggt attcgattct tgaagaacat aaagtaaata tttggtacac
     1801 agcaccaacg gcaatcagaa ggctgatgcg aatgaatatc aatccgcttc aggaatacaa
     1861 acttgataat ctgcggttaa tattgagtgt aggagaaccg cttcatgctg agggtgtaat
     1921 ttggggaaaa gaaaaattcg ggatacctat tctcgataac tggtggcaaa cagaaacagg
     1981 tggaattatg attgcaaatt acgcatctat ggaggtaaag cccggttcaa tgggaaaacc
     2041 tttaccgggt gttgaagttg ctattgcaac tattgaaagt ggaaaactga aatttataac
     2101 agaaccaaat atagaaggac accttgtact gaaaaaagga ttcccctcct tgtttcgcac
     2161 ttacttgcat gaagaggagc gttacaataa atgttttttt ggcgattggt acctaagtgg
     2221 tgacttagca aaaagagata aagacggata tttttggttt atcggtcggg ccgatgatat
     2281 aattaaaaca tcgggacaca tggtagggcc ttttgaagta gaaagtactt tgatgcgaca
     2341 ttcttttgta gcagaagcgg ccgtaattgg taagccggat ccttcaatcg gtgaattggt
     2401 caaagctttt gttgtactaa aacctggcat cgaacaaaac aatgttgtaa aaatggatat
     2461 catgggattt gccagaaaag aaatgggacc ggctatagct cctaaagaaa tagaatttat
     2521 agaaagcata ccgaaaaccc gcagcggaaa agtgttgaga agattgctga aagcacgtga
     2581 acttggatta cccgaaggtg acttgtcaac cttagaaacg ggttgatcag atattttaat
     2641 tactattaat gacaaaaaaa atgaatgcgg aaaaaggacg acaactttta tatcaaatgc
     2701 tactgataag aagatttgaa gaaaaatctg cggaacaata taccaaagca aaaatacgtg
     2761 gatttcttca cttgtatatt ggcgaggaag ctgttgctgt tggtgttatt caagcattga
     2821 cagatgaaga taatattttg agcacctacc gcgaacacgg acatgcgctt gcccgtggat
     2881 tgagtccaca atctattatg gctgaaatgt atggtaagca agaaggttgc agcatgggca
     2941 gaggaggatc aatgcacttg tttgatgcat ccaaacgatt ttatggtggt aatgccattg
     3001 ttgccggtca tcttcccctg gctgtgggaa tggcattggc ttcaaaaaaa caaaataaga
     3061 aaaatattac ctgttgtttt ttcggggaag gcgcagctgc tgaaggtgaa tttcatgaat
     3121 cgatgaatct tgctgctttg tggaatgtgc cggtattatt tatttgcgaa aacaatcaat
     3181 atgcaatggg aacagccctt aaatattcgc attcggtttt ggaaatggaa aagaagggaa
     3241 tggcctacgg tatcgaaacg gttgtggttg atggtatgaa tttaaatgca gtgatcaatg
     3301 aagccgacaa agctgttgca aaaattaaag aaactggaag gccgtatttt ttggtttgca
     3361 atacttatag gtttagagcg cactcgatgt ttgatgctga gctttaccgt gacaaaaaag
     3421 aagttgaaga atggaaacaa cgagatccta ttgcagactt tacaaattat gttttagagc
     3481 aacaattttt gactgagcag aatattctag agatagaaaa tcgtgtgaat atagaagtag
     3541 aggaagcagt tgtttttgca gaaaatggca cccgggagaa ggtggaagac ttaaccaaat
     3601 ttgtttatag taaacagaaa tgaaaacact tgagaagcaa gatataaata gttgggcctg
     3661 ccctaaatgt gggcgtgaat ttttgcgcaa ggggcaaaca cattcgtgca aaacatttcc
     3721 attggagcaa catttttcgg gtaaaactaa atggagaaaa ctatttaata aacttagtga
     3781 agccattagc acccgtatcg ggaattttaa aatagattca atagagtgtt gtattcactt
     3841 ggttcaccaa tctacgttta ctgctgtaaa ggtaatgaag gatcgtttaa gggtagattt
     3901 ttcgttggac tatgaactgt caaatgcacg aattggaaaa actgtaaaaa tgtcaaaaaa
     3961 tcgttttttg ttttatgtgg atataacaaa ggaagatgat atagataccg aattaataca
     4021 atggcttact gaggcttatt tcttaaagtg atgttgcaat cctttgaatg aaaaaaaaca
     4081 cttgatgacg ataaaaatta tttaaagttg aacaatgaat aacacaccga taacgtatcg
     4141 cgaagcacta aagcagggaa tccgtgaagc attgcaaaat gatgagcgtg tatttttgat
     4201 gggagaggat gtaggtcgct atggtggtgc ttttgcagta agtaaaggat tgctgcatga
     4261 atttggtgaa gatagaatta tggatgtgcc actttgtgaa tcgggttttg taggtgccgg
     4321 cattggagct gctatggttg gaatgaaacc aatcgttgaa gtgatgaccg taaatttcag
     4381 cctgctggca atggatcaga ttgtaaacaa tgcagcaacc atattgcaca tgtctggcgg
     4441 acaaataaat gtacctgtgg tgatacgcat gggttgcggt ataggaaggc aattggcagc
     4501 acaacattcg cacagctggg aacctttgat tgcacatatt cccggtttaa ttgccttatc
     4561 ggcaggcacg cacgaagatg ccagatatat gcttgctgaa gcacttaaaa gccccgaccc
     4621 ggttattatt ttcgaatata catccatgct taacaccgaa agggaaattc ccgaaaacat
     4681 aactcatgtg gatattagca aggcaaaggt gagaagggaa ggtaaagatg tcagcatcat
     4741 aacttatggt accggtgtgt ataaatgttt ggatgccgca aacgcattag ctgctctggg
     4801 gatagatgca gaagttgttg acttgcgtgt tttacgtcct ttggatgaag aaacaatact
     4861 tgcttctgta agcaaaactc accgggcatt aattgtagaa gatgcatgga agtcggtaag
     4921 catatcagca gaaataagtg cccgtattat ggaaaaaata ttttatgagt tggatgctcc
     4981 ggtaatgcga atttgtggag ttgaagtacc cataccttat cctatacatt tagaagatgc
     5041 atcggtacca caaaaaaatg acattattaa tgctgtaaaa aaaatgttga atcatgattg
     5101 aatttgtaat gcccagcctg ggtgctgaca tggaagacgg cacattagcg cagtggcgca
     5161 agaaggaagg cgatacggtg aagcggggcg atattattgc cgatgtagaa acacaaaaag
     5221 gattgataga aattgaattg tttgacgaag gtgttattga aaaattactg atagaaccgg
     5281 gtacaaaggt tcctgtcggt acaccattgg ctcttataaa accttcaaaa caaaaagcag
     5341 aaacaattat cactaccgct gcaaaagaag aagagattca tccaataaca gaaaaagaaa
     5401 taactcaagt tccccatgag agtttcgata aaaaatttaa aatttctcca ttagccaaac
     5461 gaatagctgc agaaaataat attgatattt ctaccttgat gggcagcggt gaaaatggtg
     5521 ttatcaccaa agaagatgta gaaaatgcaa tagctcaaaa acaaaagagt atagcaacac
     5581 aagaaacatc aggtgcagca accgaagata aaactaaaag cgcatccaac tcaatacgtg
     5641 ctgctgttgc agcagcaatg tcaaaatcga atcgcgagat accgcattat tatctcgaaa
     5701 aaacaattga catgaccaaa gctttggcat ggatgaatga gtcgaataag gaacgccctg
     5761 ttaagcaaag gttacttccc gcagcgctgc ttatcaaagc tgttgcactt tctcttcgcg
     5821 aagttcctga attgaatgct acatgggaaa acggattgca aattaaaagt gatataaata
     5881 ttggctttgt tgtttcgttg cgtggcggag gtataattgt tcctgctata cattcagcaa
     5941 atgtcaaaac ggtagatgaa attatgacca cactcaacga tattattccg agagccaaag
     6001 cactaaaact tcgcagttcc gaactcagcg attcaaccgt tatcattaca agtataggtg
     6061 aaggcggggc cgatagtgtg tatgggatta tttatccacc tcaggtggca attatcggtt
     6121 ttggtaacat tgcagaacaa ccattcgcag taaacggcat gttgggtgtt catccactgg
     6181 tacgtgctac acttgctgca gaccatcgtg cagctgacgg gctcacaggc agccgattt
//
LOCUS       DAORQA010000093         6203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3219_length_6203_cov_8.520332, whole genome shotgun sequence.
ACCESSION   DAORQA010000093 DAORQA010000000
VERSION     DAORQA010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3219_length_6203_cov_8.520332"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..2905
                     /locus_tag="PK736_05990"
     CDS             173..2905
                     /locus_tag="PK736_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32975.1"
                     /translation="MRKRILHLLSVFVLILCANANAQNVGIGIVVPTQKLHVNGNLRV
                     DGAFMPNNLPGTSGEFLMSQGAGIAPVWRNFGAISDTTNWRLIGNTIATGQFIGTLNA
                     FDFIARTANVERLRIIGVAGANQGFIGINHNAPTERLHLNGNFRLDNAFMPGNTAGAA
                     GQVLLSQGAGLAPIWRTYSTLADTVNWRLIGNNITALNFIGSLNAFDFMMRTNNVERM
                     RIVGVAGANQGFVGINVIAPSERLHVAGNMRLDNAFMPGNTAGTAGQILLSQGAGIAP
                     IWRTYSSIADTVNWRLTGNTLSTTPSFIGSLNPFDFIVRTNNVERMRVVGVAGASQGY
                     IGVNISAPSERLHVVGNVRFDGALMPNNLAGVTNQVLTSTGPGVPPQWKTLSTLADTL
                     GWQLKGNTLIGTEFIGSLNNFDFIMRTNNVPRVWLKNSGLMGVNTVFPTAYVDISSVS
                     GNRVAIRATSQDSITANFISTGLGHTGVFVNNSSAGGSAGRFQTVSAFNANPTVLINN
                     NGASNALNLLSTSGTATAAQMVVSTSASGQGINLQMLTPTSLATGIYLQQKARGKGMQ
                     IDMDSTVNTNEGLLINHKGTGTGLSIARTNTLGNGILVTLPALASGDGMVINHGGTGG
                     YGFKVTRSNTSGAGGRIVLSSGSNASTGLEIVQSGSGFGAVISTTNSAAISATNTSAS
                     GVGIYVSSNGMTPAPVVTFSLSSPIVTTNYALGDGIQTAGKGHGINSCSVEDGNSATH
                     HDGGIFYVKQSNVSNVNLAVSSVAAYIGATTYKIIGLGTVATIVRDNKGADRIMTAPE
                     TPEILFQDFGQAQLINGKAHVAFDSTLAINIKVDAKHLMKVFVQLKGDCKGVYVTNEN
                     QWGFDVVELQGGTSNVDFSWFISANRADDNINGTKIEYENNRFKKLDPGFDFRK"
     gene            2942..3343
                     /locus_tag="PK736_05995"
     CDS             2942..3343
                     /locus_tag="PK736_05995"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HRC32976.1"
                     /translation="MNPEHIIIYDGACLFCNKLIQLIYKADKRKKFYFTTNTSQTSQK
                     ISGTFHSDFSTMNSVVFVSKGKYYIKSDAVFQIALELESGWKLLYGFKIIPRFLRNVI
                     YDFVAKNRYRIFGKRQYCSIPDAAFASKLLL"
     gene            complement(3526..3906)
                     /locus_tag="PK736_06000"
     CDS             complement(3526..3906)
                     /locus_tag="PK736_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32977.1"
                     /translation="MDTQELLTELHTEHNRWQAKIKNYKDELKRWDEQLLNMVPRLQS
                     DQQSRSLERFQNQIIIQNEVLDIMRHDFKQYENEIEFIQNHKVDKTEDGINSLHDHEE
                     DKLLSFEHNFDNLRKDFKLFTGAK"
     gene            complement(3962..5377)
                     /locus_tag="PK736_06005"
     CDS             complement(3962..5377)
                     /locus_tag="PK736_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32978.1"
                     /translation="MKKITCLVLLFLSSHVLCSQTVLYSENFENLTNTFTLNGTAVNS
                     TATGENFWIVNNEFSGGSASTVCLGFPVTFSVPNTPSQPGTYAGNPYSKYLHTLSVAA
                     NTSGINNCCFLAADGLCANPENYFASMTNDISTTGYTNVQLSFGWLCDGSNNNYGEVY
                     YSIDGGSTWIQQSLPLQKYFGQGNWTTQNITNAAFDNQATLRFGFRFANQITTTAQDP
                     GFAIDEIIISGNQNVVLPIAAFTASNVSLCEKSVVDFTNLSSNATSYLWLFPGGLPAT
                     STDTNPTGIYYANYGTYSVTLIATNANGSDTLVFPNYITINQNPPAPVITLNGNLLIS
                     TPAISYQWFLNNTPIPGGVNQVQPLTSVGSYYVLITDSNGCQSASNLLVVSGIEQTSP
                     SQNFNIYTNTNHELIIDNIGLLNEPFQVSICDIEGRLVMQSTINTTTQARINIASLTE
                     GVYLTTIYNAWSQTVLKFVIK"
     gene            5721..>6203
                     /locus_tag="PK736_06010"
     CDS             5721..>6203
                     /locus_tag="PK736_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32979.1"
                     /translation="MLLLLTALVIGIISLWYTNRLVEKLSQEEKNKIELWAKALKHLS
                     DVALDAGDLTFAFDVVSNNSTIPVIQTDEKFNVKHWVNLDSVKVATDTTYLKKELAIM
                     KKQHQPIEIPFDTNMRDYIFYKDSQLLNQLKYYPFFQLAIIGLFLLVSYLAFSTSRKA
                     E"
BASE COUNT         1892 a         1098 c         1298 g         1915 t
ORIGIN      
        1 atatggcatt tataaaaaaa gatgatttaa aattgaataa ttccgtagaa ttaaatttac
       61 taaacattgc tccgggcaat tatgttgtaa gaattactga cggagtttct actttggaat
      121 ctgtaaaact tatggtgcag aagtaacaga tttcgtttat taaaaaacac aaatgagaaa
      181 aagaatttta catttattaa gcgtttttgt tttgatttta tgtgcaaatg caaatgctca
      241 aaatgtaggt attggcattg ttgttccaac acaaaagttg cacgtaaatg gcaatctgcg
      301 tgttgacgga gcatttatgc ccaataattt accgggtacc agtggagagt ttttaatgtc
      361 gcagggagca ggaattgctc ctgtatggcg caactttggc gcaatttccg atactactaa
      421 ttggaggctt attggtaata caattgctac cggtcaattt attggaacgt taaatgcctt
      481 cgattttata gcacgcactg caaatgtaga acgcttgcga attattggag ttgccggtgc
      541 caaccaagga ttcataggta taaaccataa tgcccctacc gaaagactac atcttaacgg
      601 aaactttcgt ttagacaatg cgtttatgcc tggcaatact gccggtgcag cggggcaagt
      661 attgctttcg caaggtgcag gtttagcgcc catttggcgc acttattcta cacttgccga
      721 tacagtaaac tggcgtttga ttggaaacaa tattaccgca ttaaatttta ttggctcgct
      781 caatgcattc gattttatga tgcgcacaaa taatgtagaa cgcatgcgta tagttggggt
      841 tgctggtgca aatcaaggct ttgtaggaat caacgttata gcgccaagcg aacgattaca
      901 tgttgcggga aatatgcgtt tagataatgc gtttatgccg ggtaatactg ccggcacagc
      961 ggggcaaatt ttgctttcgc aaggtgcagg cattgctcct atttggcgca catattcttc
     1021 catcgcagat acagtaaact ggcgattaac agggaatacc ctttccacca ctccaagctt
     1081 tattggatca ctcaatccat ttgattttat tgtgcgtaca aataatgttg agcgtatgag
     1141 ggttgttggc gttgcgggtg cgagtcaggg ttatataggt gtaaatataa gtgcgccaag
     1201 cgagcggttg catgtagttg gcaatgttcg ttttgacggt gcattgatgc cgaataattt
     1261 agctggtgta actaatcagg tactcacttc aacgggcccc ggagtgccac cacaatggaa
     1321 aacactatca actttagccg acactttagg gtggcaattg aaaggcaaca ctttaatcgg
     1381 aaccgaattt attggctcgc tcaataactt tgattttatt atgagaacca ataatgtgcc
     1441 cagggtatgg ttaaaaaaca gtggtttgat gggtgtgaat acagtttttc caacagcata
     1501 tgtcgatata agcagcgttt cgggtaacag ggttgcaata cgtgctactt cgcaagatag
     1561 tatcactgca aactttattt ctacaggctt ggggcatacc ggagtttttg taaataattc
     1621 ttctgctggt ggtagtgcag gaaggtttca aacagtaagt gcattcaatg ccaacccaac
     1681 tgtacttata aataacaatg gtgcgtctaa tgcgctcaat ttattgtcaa catcgggcac
     1741 agcaaccgct gcacaaatgg tagtttccac atcagcatcg gggcaaggaa taaatttaca
     1801 aatgttaaca cccacaagtt tggcaaccgg aatttatctg caacaaaaag cacgcggtaa
     1861 aggaatgcaa attgatatgg acagcactgt aaatacaaac gaagggttat tgataaatca
     1921 taagggaacc ggaacaggat tgagtattgc ccggactaat acccttggca acggcattct
     1981 tgttacgctt ccggcattgg ccagtggcga tggtatggtg ataaaccatg gtggtacagg
     2041 tggatatggt tttaaagtaa cacgttctaa tacttccggt gcaggtgggc gaatagtact
     2101 tagtagtggc tccaatgcaa gtacaggttt agaaattgta cagtcgggca gtggttttgg
     2161 tgctgttatt tcaaccacaa attctgcagc tatttctgcc acaaatacaa gtgccagtgg
     2221 tgtgggaata tatgtttctt caaatggaat gactcctgcg cctgtggtaa cattttcgct
     2281 ttcatcgcct attgttacta caaactatgc cttgggcgat ggtatacaaa ctgccggcaa
     2341 aggacatgga ataaactcat gcagtgtaga agatggcaat agtgcaacac accacgatgg
     2401 gggtattttt tatgtaaagc aatccaatgt aagtaacgtt aatctcgcag taagttctgt
     2461 agctgcctat ataggcgcta ccacttacaa aataataggt ttgggcactg tagcgaccat
     2521 tgtgcgcgat aataaaggtg ccgatagaat tatgaccgct cccgaaacac ctgaaatttt
     2581 atttcaggat tttggtcagg cacagttaat aaatggcaag gcacatgttg catttgattc
     2641 tactttggct atcaatatta aagtggatgc caaacatttg atgaaagttt ttgttcagct
     2701 aaaaggagat tgcaaaggtg tgtatgttac caatgaaaat cagtggggtt tcgatgtggt
     2761 ggaactgcag ggcggaacga gtaatgtaga cttcagttgg tttatatctg ccaatcgtgc
     2821 tgatgataat attaatggaa ctaaaataga gtacgaaaac aatcgtttca aaaaacttga
     2881 ccccggattc gatttccgaa aataaaaaac aacgtataca gttatagtta atttccaaat
     2941 tttgaatccc gaacatatta tcatttacga tggcgcgtgt ttgttttgca acaaacttat
     3001 tcaattgatt tataaagcag ataagcgtaa aaagttttat ttcaccacca atacatctca
     3061 aacatcacaa aaaattagcg gcacgtttca ctcagatttc agtacaatga actctgtggt
     3121 ttttgtttcc aaaggcaaat attacatcaa gtcggatgct gtttttcaaa tcgcattaga
     3181 acttgaatcg ggatggaaac tgttatacgg gtttaagatt attccgcgtt ttttgcgcaa
     3241 cgtgatttat gattttgttg caaaaaaccg ttatcggata tttggaaaga gacaatattg
     3301 cagcatccca gatgcagcat ttgcaagcaa gctgctatta taattttagt gacaagcaaa
     3361 tccggtatta tatcaacagc ttttagtttc aaaaaaaatg cagaaccatg gttctgcatt
     3421 ttaataaaat tttggctaac aagaattgga ggccacaaat tcaaaaacaa tagttttgat
     3481 attgattatt aataattccg ttatggaatg tgattttttt tcttgctact tcgctccggt
     3541 aaagagttta aaatctttgc gaaggttgtc gaaattatgc tcaaacgata ataatttatc
     3601 ttcctcatga tcatgtaagg aattaatacc gtcttcggtt ttatccacct tatggttttg
     3661 aataaattca atttcgtttt cgtattgttt aaaatcgtgc cgcataatat ccaatacttc
     3721 attttgaatt attatttgat tttgaaatcg ttccaaactt cggctttgct gatcgctttg
     3781 taaacgcgga accatattaa gcaactgctc gtcccatcgt ttcagttcgt ctttataatt
     3841 tttaattttt gcttgccacc gattgtgttc agtgtgcaac tcagtaagaa gctcttgtgt
     3901 atccatactt gaaaattatt taaaattaaa cttagaggaa ttaaattagt atcaaatgat
     3961 gttatttaat tacaaacttc aaaacggttt gtgaccaagc gttatatatt gtagttaaat
     4021 aaaccccttc tgtaagcgaa gcgatgttta ttctcgcttg tgtggttgtg ttaatagtgc
     4081 tttgcattac caaccggcct tcgatgtcac atattgaaac ctggaagggt tcgtttaaca
     4141 atccaatatt atctattatt aattcgtgat ttgtatttgt ataaatgttg aaattttgtg
     4201 atggcgaagt ttgctcaatg cccgaaacca ccaacaggtt cgaagctgat tggcaaccat
     4261 tgctgtccgt tattaaaaca tagtaactac caacactggt aagaggttgc acttgattta
     4321 cgcctccggg aataggggta ttatttaaaa accattgata ggaaatagct ggagttgaaa
     4381 tgagcaaatt gccatttaaa gttataaccg gtgccggagg attttgattt atggtaatgt
     4441 aatttgggaa aactaaagtg tcgctgccat ttgcattagt tgcaataagc gttaccgaat
     4501 aggtgccgta attagcataa taaattccgg taggatttgt atcggtagaa gtagccggca
     4561 aaccgcccgg aaacaaccat aagtaagaag tggcattgct ggacagattg gtaaaatcga
     4621 ctacactttt ttcacataaa ctaacattac tggcggtaaa tgctgctata ggcaacacta
     4681 cattttggtt gccgcttatg attatctcgt caatagcaaa accgggatcc tgagcagtag
     4741 ttgttatttg atttgcaaaa cgaaaaccaa accgcaatgt ggcttgatta tcgaaagctg
     4801 cattggtaat gttttgtgtg gtccagttac cctgaccaaa atatttttgc aatggcagcg
     4861 attgttgaat ccatgttgaa ccgccatcta tactataata tacttcgcca taattattat
     4921 tgctgccatc gcacaaccag ccaaaactca actgtacgtt ggtatatcct gttgtactaa
     4981 tatcgttggt catagaagcg aaataatttt cgggattggc gcataaccca tcggcagcca
     5041 aaaaacaaca gttgtttatt ccgcttgtat tggcagcaac acttaatgtg tgtaaatatt
     5101 tgctataagg attaccggca taagtaccgg gttgcgaggg tgtattggga acactaaaag
     5161 ttacaggaaa accaagacaa acagtggaag cgctgccgcc cgaaaattcg ttatttacaa
     5221 tccaaaaatt ttctccggtg gcggtactgt ttacagctgt gccattgagc gtaaatgtgt
     5281 ttgtaagatt ttcgaaattc tcgctataca aaacagtttg cgaacacaaa acatgtgatg
     5341 ataaaaacaa taaaacaaga caggtaattt ttttcatatt cataatttta tttgaataca
     5401 atattaactt tttttaatta aatggagaaa gacccggcct taaaataatg aacatttttt
     5461 agggatttta aacaccattt tttagtgcac aggtcattcc gtaatataag tcaaattgat
     5521 tttgtccact tacaaagcat aaactaatta aaaggttttc ttaaagtaaa cgtttagctt
     5581 ttatagcggt gtagtgcgcc gggtcgggtt ttaatggtta ttaagcactt gggggtgggt
     5641 atttttaggc atgtttgtta atattgcacc ctaatttcga tacagagttg aatatataca
     5701 ctcgtaagca aagatggaaa atgttattac tgctaacagc gttggttatt ggtattatat
     5761 cgctgtggta tactaaccgg ttggtagaaa agctttcgca ggaagaaaaa aacaaaatcg
     5821 aactttgggc taaggcgcta aaacacttat cggatgtggc actcgatgct ggtgacctca
     5881 cttttgcttt tgatgtggta agcaacaaca gcaccattcc ggtaatacaa accgatgaga
     5941 agtttaacgt aaagcactgg gtgaacctcg atagcgtaaa agttgcaacc gatacaactt
     6001 acctgaaaaa ggagttggcc attatgaaaa agcagcatca gcctatcgaa ataccatttg
     6061 ataccaacat gcgcgattat attttttata aggattcgca attgcttaat cagttaaaat
     6121 attacccgtt ttttcagtta gccattattg ggttgttcct gttggtttca tatttagcat
     6181 tcagcacatc gcgcaaggcc gag
//
LOCUS       DAORQA010000094         6105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3300_length_6105_cov_11.430909, whole genome shotgun sequence.
ACCESSION   DAORQA010000094 DAORQA010000000
VERSION     DAORQA010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3300_length_6105_cov_11.430909"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1936)
                     /locus_tag="PK736_06015"
     CDS             complement(<1..1936)
                     /locus_tag="PK736_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRC32980.1"
                     /translation="MRKIILFLFIFLFAGITESHAQFSRYIVQLKDKGTNPFSISNPL
                     QYLTQRSIDRRIRYGIAIDSSDLPITPRYIDSIRLAGAVTILNSSKWLNQVAIQTTDA
                     AALAKINSFPFVISTGPVATRNQQNDKPINKKLDGPDNTIVPPPASPQGINDFYNYGV
                     SFGQVHLNNGEFLHNRGFRGQGMQMAVLDAGFFHYLSLPTFDSMRNNGQVLGTWDFVA
                     GNASVDEDHPHGMNCLSTIAANMPGVFIGTAPKTSFYLYRTEDISSEYPIEEQNWIAG
                     IERADSLGIDISSTSLGYFNFDNAIFNHTYADMDGNTTMIARAADLAAKKGIMIVVAA
                     GNEGTSSWHFIISPGDGDSVMTVGAVSASGVVGNFSSYGPSSDGQIKPNVAAVGVGAV
                     IANSSNGQPTFGNGTSFACPNMAGLTSCLMQAFPEINNMGIMNAMQQSANRATNPDNR
                     TGYGIPDMKKAFVMLIKQQYTQMATVANCSVNLQWTAKTDSVISITVERKLPAETDYT
                     SISTHNSTGAFAARNFSYADDLRNFPASGIRYRFKMTIAADTSFYLDSITVNFTPRPD
                     LGADKSISVCGTGVVDLTTQFNTTGLAANWTLGGAAVANPAAVTVAGTYQLIGSNNSG
                     CSDTALVIFSIAAKPDLGTDK"
     gene            complement(2082..>6105)
                     /locus_tag="PK736_06020"
     CDS             complement(2082..>6105)
                     /locus_tag="PK736_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32981.1"
                     /translation="IYSSTGNNAKNINDSIVNCNLHEFSSWTIYLNDSALQWKIIGNS
                     FYKQTPLVPRNMVGGDAIIKIEESYDPVGHVISNNYFGGTGPQCTGGQMQVNYNVFDF
                     GIIRGYGRVAISNNYFRRINFAGTADISLIKMGISTTSPVHPAYGTISNNQFGGIDPA
                     DSINFQNTYSTPNSNYPIYAGFILIAGGVQAVANEFNHIRFWAADSTFIINVKVIEGC
                     YSVRDNIIGNPAVYNSIINNTNGGMLMISGGHAINNTISNITNTSTLHSTVSSPIIRG
                     IVTNSDSVYYNKILRLTNSPSIANSSSQYFTSMIGITAGLAPGNNPNHIVGNVVHSLI
                     NNGESGDITAIQANGAQVSKNLVHSLFITNPANIYSTIYGISSNPDSVTNNMILLGQD
                     SAGNSMTLPVNIRGIHSGRNVLHNTVAIVGTNVADVSGRFSFCYGAGNNGPSQANIKN
                     NIFYNTRSNQSAASANRHYCIWINNTSDQSDYNLFYNTGTGAYFGFNSGPLVAYPTFA
                     AWKAGTGKDLNSVYGDPLLINTAGSYSNVDLHLSNWTPAETAGTATATLATDFDNEVR
                     SAQSPVDIGADAGNFGQCAVANAGSDTTICAGASVQIGTAQLPNHTYSWTSNPAGFVS
                     NLATPVVSPTVNTVYLLTLTNANSTCTTTDSVKVFISAPVTPTITINTSTTTICSGTT
                     ITFTSSVTGGGTNPSYQWKINNVNAGTNNSAFSTSTLNNGDQVSCILTSNASCVTSTT
                     ANSNIISMIVDPSVMPSVTISTPNNSICAGTPVTFTATPVNGGAAPIYHWWLNGIHVG
                     NNSNTFTSSTLNNNDQIMASLKSSIVCHTPDTVWSNIISMSVTSIPNAYAGNDTAICA
                     GNSVQLNGSGGAAGAAYSWSPAAGLSNPNIANPVASPAVTTVYVLTVSNSPSCFAKDT
                     MVITVNPAIPASVSINASTNNICSGTPVTFTATASNGGSSPQFIWYINGSSVSIGSNL
                     FTSSSLSNNDQVMVKMISDLSCASPDTAWSNIVTMIITPLPVASAGNDVTICTGTGIQ
                     LNGSGGSAGSTYLWSPATGLSNPNIANPVASPVATTAYILTVSNSTACFAKDTVLVSV
                     SSAVSPAVNINTANSNICLGTAAVFTAIPSNGGNNPSYQWRVNFANAGSNSNTFTSNI
                     LNNNDNVQVILTSSLTCAVPDTAGSNIITMSVSQLATPLISVNNDVLKVTNQDIAAIY
                     TWQIKNGTAWADIIPTATGTVYTATAAGEYRVKAVKGACTEYSASQVTNFSALYSHFI
                     ELYPNPAHNYITLDSIALIKKYETVEIIDIHAKRVLPLFNVTNRTSITIDISILNNGI
                     YMVIIRQRDGSYSAIKFIKQ"
BASE COUNT         1716 a         1242 c         1316 g         1831 t
ORIGIN      
        1 acttatcagt gccaagatcg ggttttgctg caatgctgaa aattaccaaa gcagtatccg
       61 aacatccgga attgttactg ccaattagtt ggtaggtgcc tgcaactgtt actgctgcag
      121 ggtttgcaac agcagcgcca cccaatgtcc agtttgctgc aagtccggtt gtattgaatt
      181 gtgttgtaag gtcaacaaca cctgtaccgc aaacgctgat gcttttatct gcacctaaat
      241 ccggcctggg cgtaaaattt actgtaattg aatcaagata aaaactggta tctgcagcaa
      301 tggtcatttt aaagcgatac ctgattccgc tggctggaaa attccgcaga tcatcagcgt
      361 atgaaaaatt tcttgctgca aatgcgcctg tggaattatg tgtactgatg gaagtataat
      421 cagtttcagc aggtaattta cgttctactg taatgctgat aaccgaatct gtcttagcag
      481 tccattgtaa gttaacactg cagttagcca ctgtagccat ttgtgtgtac tgttgtttta
      541 tcagcatcac aaaagctttt ttcatatccg gtatgccata gccagtgcgg ttgtcggggt
      601 ttgtggccct gttggcagat tgctgcatgg cattcattat acccatatta ttaatttcgg
      661 gaaaagcctg catcaggcag ctggtaagcc ccgccatatt ggggcaggca aaagaagttc
      721 cgtttccaaa agttggttgc ccgtttgagg agttggcaat cactgcacca acaccaactg
      781 cagcaacatt tggttttatt tgcccgtcgc tgcttggccc gtaactgctg aaattaccaa
      841 ccacgccact tgcagataca gcgccaacag tcattacact atctccatct ccgggcgaaa
      901 ttataaaatg ccatgaagaa gtcccttcat ttcctgcagc caccacaatc attattcctt
      961 tttttgctgc aaggtctgca gcccgggcaa tcatcgtggt attgccatcc atatctgcat
     1021 aggtatgatt gaaaatggca ttgtcgaaat taaaatagcc caaagaggta gaagaaatat
     1081 caataccaag gctgtctgct ctttcaatac ctgcgatcca gttctgttct tcaatcgggt
     1141 actccgagct tatgtcttct gtgcggtata aataaaaaga tgttttgggg gcagtgccta
     1201 taaaaacgcc gggcatattt gcagcaatcg tactcaggca gttcatgcca tgcggatggt
     1261 cctcatcaac acttgcatta ccggcaacaa aatcccaggt gcctaaaacc tgcccgttat
     1321 tccgcatgct gtcgaaagta ggcaggctta aataatgaaa aaatcctgca tccagaacag
     1381 ccatttgcat tccctgtccg cggaaaccac ggttgtgtaa aaactcaccg ttattcagat
     1441 gcacctggcc aaaagatacg ccgtaattat aaaaatcatt gataccctgc ggtgaggcgg
     1501 gaggaggaac gatggtatta tccggcccat ccaacttttt atttatgggt ttatcgtttt
     1561 gttgattcct tgttgcaacc gggccggtac tgataacaaa agggaagctg ttgatctttg
     1621 ccaaagctgc cgcatcggtt gtttgaatgg caacctggtt cagccatttg gaactgttga
     1681 gaatggttac ggctccagcc aaacggatac tgtcgatata tcttggggtg atgggaagat
     1741 ctgaggaatc tattgcaata ccatatcgga ttcttctgtc gatggaacgt tgggtaagat
     1801 attgtaatgg attgctgatg gaaaatggat tggtgccttt gtcttttagt tgaacaatat
     1861 aacgggaaaa ctgggcatgg ctttctgtaa tacctgcaaa caaaaagata aaaagaaaga
     1921 gtataatttt tctcatcgga taggtttaat gattatttcc aatacatgtt taacgattga
     1981 attgaatttt aaaattacgt tatttaatgt aaattagttt tatgcaagaa agcctttcga
     2041 ttaattacga aaggctttct ttttagtttg aacaggttta tctactgctt aataaatttt
     2101 attgctgaat aagagccatc tctttgcctg attatgacca tataaatacc attattcaaa
     2161 atacttatat cgatggtaat gcttgtgcgg tttgtaacat tgaataaagg cagaactctt
     2221 ttggcatgta tatcaatgat ttcaactgtt tcgtattttt ttatcaaagc aattgaatca
     2281 agtgtaatat agttatgagc cgggttaggg tataattcaa taaaatgtga atacagtgca
     2341 ctaaagttcg ttacctgaga agcagaatat tctgtacaag cccctttaac tgcttttacc
     2401 ctgtactcgc ctgcagccgt tgcagtataa acagtgccgg ttgctgttgg aattatgtcg
     2461 gcccaggcag tgccattttt tatctgccag gtatatattg cagcaatatc ctgattggta
     2521 actttaagta catcgttatt tacagagatc agcggcgttg ccaactgcga tacagacatt
     2581 gttatgatat tgcttccggc agtatcagga acagcacagg taagcgaact ggttaaaata
     2641 acctgcacat tgtcgttgtt attgaggatg ttgctggtaa atgtattgct gtttgagccc
     2701 gcatttgcaa aatttacccg ccattgataa gatgggttat tgccgccatt ggaaggaata
     2761 gcagtaaaaa cggcagcggt acccaaacaa atattactgt tggcagtatt aatatttact
     2821 gcaggagaaa cagcactgct aacgctgacc aacactgtgt cttttgcaaa acaggccgtg
     2881 ctgttcgaca cggttaatat ataagccgta gtagcaacag gactggcaac cgggtttgca
     2941 atatttggat tactgagacc cgtagcaggc gaccataaat atgttgaacc tgcagaacca
     3001 cccgatccat tcagctgaat accggtgcct gtgcagattg ttacatcatt gccggcactt
     3061 gccacaggta atggagtaat aatcattgta acgatattgc tccatgctgt atcaggagaa
     3121 gcacaggaaa gatcactaat cattttaacc attacctggt cattgttact taaactgctg
     3181 cttgtaaata aattacttcc gatacttacc gaactgccat taatatacca aataaattgc
     3241 ggagagctgc cgccattgga tgctgttgct gtaaaagtta ccggtgtgcc cgagcaaata
     3301 ttattagtgc ttgcattgat gcttacagaa gcaggtattg cagggtttac agtaattacc
     3361 atggtatctt ttgcaaaaca acttgggcta tttgatacgg tcaatacata gactgtagta
     3421 acagcagggc tggctacagg gtttgcaatg tttggattgc ttaaaccagc ggccggcgac
     3481 catgaataag ccgctccggc cgctcctccg gacccattga gttgaacact gttaccggca
     3541 caaattgctg tatcattacc tgcgtaagca ttgggtattg aggtaacaga catgctgata
     3601 atattactcc agactgtatc gggggtatgg cagactatag aactttttaa agaagccatt
     3661 atctggtcat tattatttaa ggtgctgctt gtaaaagtgt tgctgttgtt tccaacatgt
     3721 attccattaa gccaccagtg gtatatcggt gctgcgccac cattaacagg cgtagctgta
     3781 aaggtaactg gagtacctgc acaaatgctg ttattcggag tgctgatagt tactgacggc
     3841 attaccgatg ggtctactat catggaaata atattactgt tcgctgttgt gctggtaaca
     3901 caagatgcat tacttgtaag tatgcaacta acctggtccc cgttattcag ggtgcttgtg
     3961 gaaaatgcgg aattgtttgt accggcattt acgttattta ttttccattg atatgaaggg
     4021 ttagttcctc cacctgtaac agaacttgta aaagtaatag tagtgcctga acagattgta
     4081 gtcgttgatg tatttattgt tatggtaggg gttacaggag cactgataaa aacttttacc
     4141 gaatcagttg tagtgcatgt tgaatttgca ttggtcaacg taagtaaata aactgtatta
     4201 acagttgggc ttactaccgg agttgcaaga tttgaaacga atcctgccgg gtttgatgtc
     4261 cagctataag tatgattcgg tagttgtgca gtaccaattt gaacgcttgc tcctgcacaa
     4321 attgttgtat cacttcctgc attggcaaca gcacattggc caaagttacc agcatcagca
     4381 ccaatatcaa caggactttg tgcagaccgg acctcattat caaaatctgt ggccagggta
     4441 gctgtcgcag tgcctgcagt ttcggccgga gtccagtttg ataagtgcag gtccacgttc
     4501 gaataactgc ctgccgtgtt gataagtaat ggatctccgt aaacagaatt caggtcttta
     4561 ccggttccgg ccttccaggc agcaaatgtc gggtaagcta caagagggcc actgttgaat
     4621 ccaaaataag caccggtgcc cgtattataa aacaggttat agtcggattg gtctgaagtg
     4681 ttatttatcc agatacaata atgcctgttt gcacttgctg ccgactgatt tgaacgtgta
     4741 ttgtaaaata tattgttctt aatattggcc tggctgggac cgttattgcc ggcaccatag
     4801 caaaagctga acctgccaga tacatccgcc acatttgtgc caacaatagc aactgtatta
     4861 tgaagaacat ttcttcctga gtggattcct ctaatgttta ccggcaatgt catactgttc
     4921 cctgccgaat cctggcccag gaggatcata ttattggtga ctgaatcggg attactactg
     4981 ataccataaa ttgtgctgta aatattagcc ggattcgtga tgaacaatga atgcaccagg
     5041 tttttgctta cttgggcgcc attagcctgg atagcagtaa tatcaccgct ctcaccatta
     5101 ttgatcagtg agtggactac atttcctact atgtgattag gattattgcc tggagcaagc
     5161 cccgccgtga taccaatcat tgatgtaaaa tattgactgc ttgagttggc aatactgggg
     5221 ctattagtta accgtaatat tttattataa tatacactat ccgagtttgt cacaatgcct
     5281 cgtataatag ggctgcttac cgtgctatga agggtggagg tatttgttat atttgaaatg
     5341 gtattattta tagcatgtcc gccagaaatc ataagcatcc caccatttgt attattgatg
     5401 atgctgttgt atacagctgg atttcctatt atattatccc gtacgctgta acagccctct
     5461 atcactttca cattaattat aaaagtagaa tcagcagccc aaaacctgat atgattaaac
     5521 tcattggcca ctgcctgaac accaccagct ataagaataa atcccgcata aattggatag
     5581 ttagagttgg gggttgaata ggtgttctgg aagttaatac tatctgccgg gtcaataccg
     5641 ccgaattggt tattggaaat tgtaccatac gccggatgca caggcgatgt ggtactgatg
     5701 cccatcttta taagagaaat gtctgcagtt cctgcaaagt tgatcctcct gaaataatta
     5761 ttggaaatag ccactcgccc atacccccga atgataccga aatcaaaaac attataattc
     5821 acctgcattt ggccaccggt acattgagga ccggtacctc caaaataatt attgctgatt
     5881 acatgcccta caggatcata actttcttca atcttaatta ttgcatctcc accaaccatg
     5941 tttcggggaa caagaggtgt ttgtttataa aaactattgc caataatctt ccattgtaat
     6001 gccgagtcat tcagataaat ggtccaggaa gaaaattcat gaagattaca atttacaata
     6061 ctatcattaa tgttttttgc gttgtttcca gtactactat agatg
//
LOCUS       DAORQA010000095         6089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3313_length_6089_cov_15.095956, whole genome shotgun sequence.
ACCESSION   DAORQA010000095 DAORQA010000000
VERSION     DAORQA010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3313_length_6089_cov_15.095956"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="PK736_06025"
     CDS             <1..1296
                     /locus_tag="PK736_06025"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32982.1"
                     /translation="FADVDNNIITFYDADITGPNANLVRYGIRASGGFGLHTINHNWI
                     RKPGNTPTSNLKSKLFGINVQSTENNFVQNNLVEKTGTGIRFFNTSIINSLLCNNMDG
                     NRFGLRLENANIGNQGSLTSTQDNTWSNITFDVEGLGTLFPTSFYAKSNNQPWTPPFG
                     KANPPNTYFFPSGTSQSNCDVFYPQPLRDHILDIVNQDGDFNLLTPEEQYAANQVAFE
                     KLHNDSSLMNKGDTDDAVLQNYHDSIATTNIGQLANGVNLNSSIFSSISPVNNAEENM
                     KTLLGIYAVTWGIDIFDFTTGQFDALYNIASQNPITGGNAVYAARGMLHKFFDDDMMA
                     NGKLIHPVRLINLKPYKKGKLYPNPANDFAYYRIRLKKDETCTMQLIDLSGKILSSAS
                     NKNENCLMPIQTANISQGIYCVRILINGMPFENHKLVKQ"
     gene            1293..2240
                     /locus_tag="PK736_06030"
     CDS             1293..2240
                     /locus_tag="PK736_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32983.1"
                     /translation="MKFVFILIFIFLAQGFGISQNLVPNGSFEDTLPYNSNPCPWCID
                     SVNPWFMGLDDQSPDYFNAVYFPAWIGNGVPKNGVGYQHAKDGVAYAGLVPYSNFSVF
                     REYLSVLLSDSLITNHFYLLRFYVSLADSSKHAIDRLGCYFSSDTPNFGNLNFNYFPQ
                     VANTLGNIITDTMNWIPIEGYYHALGGERILTFGNFATDTLTQFIQFSYNNPWYTCYY
                     YLDDVSLIDCTATFIQEIELPQFNLFPNPATDILNIQSEQGFARLLFYNSVGDVALQF
                     SQTLETNFACDVAILPKGIYIVAIEDKYGRMVYKKFIKM"
     gene            2253..>2555
                     /locus_tag="PK736_06035"
     CDS             2253..>2555
                     /locus_tag="PK736_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32984.1"
                     /translation="MKYFIILLLIIGSFISVKSQNLVPNGSFEDTIPYNTNPCPWCID
                     SVNPWFMGLDDKTPDYFNGVYLPAWIGNGVPKNAVGYQSAKDGVAYAGLVPYSNFSV"
     assembly_gap    2556..2655
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3005..3871)
                     /locus_tag="PK736_06040"
     CDS             complement(3005..3871)
                     /locus_tag="PK736_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRC32985.1"
                     /translation="MLFTWTNTSVKESNRFIWFEKWIIGRQTLQQIQQESGYSISTLK
                     RYFYKYLDKPPQWKVYPSEKVNLMIDGTYFSNDLCLIIYRDNTIKFTQLYRISNGEWY
                     DELKEDLENLHALGVKIESITCDGHKAILKAVRRSSNGATLQRCLVHIQRMCRIWLTK
                     RPLSDAGKELRIIISQLHLIQTAFERDCWIVSMVRWYEKYELFINEKSFNPTSKRYWY
                     KHKLVRRSFMTIKNALPDMFHYLDNPRIPKTTNGLESFFGHLKNHLTVHRGLSANHRK
                     NFLKWYLSLKNK"
     gene            complement(4095..4352)
                     /gene="rpmA"
                     /locus_tag="PK736_06045"
     CDS             complement(4095..4352)
                     /gene="rpmA"
                     /locus_tag="PK736_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRC32986.1"
                     /translation="MAHKKGAGSSRNGRESHSKRLGIKIYGGQVAIAGNIIVRQRGTK
                     HFPGINVGLGKDHTIFALVDGKVQFRKKRDEKSYVSIVPFS"
     gene            complement(4455..4766)
                     /gene="rplU"
                     /locus_tag="PK736_06050"
     CDS             complement(4455..4766)
                     /gene="rplU"
                     /locus_tag="PK736_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRC32987.1"
                     /translation="MYAIVDIAGQQFKVSKDQSVFVHRLKADEGAEVEFDKVLLVDND
                     GSITVGAPTLSGARVTAKVINHLRGDKVLIFKKKRRKGYQKCNGHRQDLTKIQIANIQ
                     L"
     gene            complement(4871..5785)
                     /locus_tag="PK736_06055"
     CDS             complement(4871..5785)
                     /locus_tag="PK736_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRC32988.1"
                     /translation="MLSRFPKYLKWILLFLLTLIWGSSFILMKEGLMAFTPIQVAGMR
                     MVFAASALLPFIIRHVGKIEPSKWKWIIAGGLIGNGIPAFLFTLAETRVTSTTAGMLN
                     SLSPVFTLLIAWLIFKNKPRITQTIGVLIGMIGALALIYFQPGSTGINADPYAWYVVL
                     ACLGYGCSVNIIRNHFGGMNPVHAAGFALAFAAIPYFLYFLGSGFFTTVGNQPYFWRA
                     FASVAVLGVMGSALATVLFNMLVKASSALFAASVTYLMPIVSLSLGVLYGESVHQMQM
                     IGLAVILGGVYLISLKPKPKALKSGQAN"
BASE COUNT         1876 a         1087 c         1086 g         1940 t
ORIGIN      
        1 tttgccgatg tagataataa tataatcact ttttacgatg ccgatatcac agggcctaat
       61 gcaaacttag tgcggtatgg catacgtgca tcgggcggat ttggcttgca taccatcaat
      121 cacaattgga tacgtaaacc cggcaataca ccaacatcaa atttaaaatc aaaattattt
      181 ggcataaatg ttcaatcgac tgaaaataat tttgtgcaaa ataacctggt agagaaaacc
      241 ggaacaggga ttagattttt taatactagt ataataaatt cacttttgtg caataatatg
      301 gatggcaacc gttttggttt gcgattggag aatgccaata ttggaaatca agggtcttta
      361 acctcgacac aagataatac ttggtcgaat ataacttttg atgttgaggg gcttggtact
      421 ctttttccga cctcttttta tgctaaatca aataatcaac cttggacacc gccttttgga
      481 aaggcgaatc cgccaaacac atattttttt ccatccggaa cttcacaaag taattgcgat
      541 gttttttatc cacagccctt gcgtgatcat attttggaca ttgtaaatca ggatggggat
      601 tttaatttat tgactcctga agaacagtat gctgcgaacc aagttgcatt tgaaaaacta
      661 cacaatgatt catcgttgat gaataaagga gatacagatg atgcagtttt gcaaaactat
      721 cacgacagta ttgcaactac taacattggg caattagcaa atggggtaaa tttgaacagt
      781 agtattttta gcagcatttc gcccgtcaat aatgccgaag aaaatatgaa aaccttgctc
      841 ggaatatatg cggtaacatg gggtatagat atatttgatt ttactaccgg gcaatttgat
      901 gcattgtata atattgcttc acaaaatccc attaccggtg gcaatgctgt atatgctgca
      961 aggggaatgc ttcataaatt ttttgatgat gatatgatgg caaatggaaa attaattcat
     1021 cctgttagac taataaattt aaaaccatac aaaaaaggca aactctaccc aaacccagca
     1081 aacgattttg catactatcg tattcgattg aaaaaagatg aaacgtgcac tatgcaatta
     1141 atcgatttgt cgggtaagat attaagcagc gcttccaata aaaacgaaaa ttgtttgatg
     1201 ccaatccaaa cagcaaatat ttcgcaagga atttattgtg tgcgcatttt aattaacgga
     1261 atgcctttcg aaaatcataa gttggtaaaa caatgaaatt cgttttcatt ttgatattta
     1321 ttttcttggc acaaggtttt ggaatcagcc aaaaccttgt gcccaatgga agtttcgaag
     1381 atactttacc gtacaattca aatccttgcc catggtgtat tgatagtgta aatccttggt
     1441 ttatgggtct ggatgatcaa tctccagatt atttcaatgc tgtttatttt ccagcttgga
     1501 taggtaatgg tgtgccgaaa aacggagtgg gctatcaaca tgcaaaagat ggagttgcgt
     1561 atgctggctt ggttccctat agtaatttta gcgtatttcg tgagtactta tctgttttgc
     1621 tatcggattc actgattact aatcactttt atttgctccg gttttatgtt tctttggcag
     1681 atagcagtaa acatgcaatc gataggctag gttgttattt ttcttctgac acacctaatt
     1741 ttggtaattt aaatttcaat tattttccgc aagttgcaaa cacactaggt aatataatca
     1801 cagatacaat gaattggata ccaatcgaag gttattatca tgcgcttggt ggtgaaagaa
     1861 tactgacatt tggcaatttt gcaactgata ctttaactca atttatccaa ttcagttata
     1921 acaatccatg gtatacttgt tactactatt tagacgatgt ctccctaata gattgtacag
     1981 ccaccttcat acaagaaata gaactgccac agtttaattt atttccaaac cctgcaaccg
     2041 atattttaaa tatacaaagc gaacaagggt ttgctcgttt gttgttttat aatagtgtgg
     2101 gtgatgtagc tttacaattt agccaaacac tcgaaactaa ctttgcatgt gatgttgcca
     2161 tattgccaaa aggaatttat atagtagcaa ttgaagataa atatgggaga atggtttata
     2221 agaaatttat aaaaatgtag tggacgaaaa aaatgaagta ttttatcatt ttacttttaa
     2281 ttattggttc atttataagt gtgaaaagcc aaaaccttgt gcctaatggt agttttgagg
     2341 atacaattcc atataacaca aacccgtgtc catggtgtat tgatagtgta aatccttggt
     2401 ttatgggttt ggatgacaaa acgccagatt attttaatgg tgtttatttg ccggcatgga
     2461 ttggcaatgg tgtgccgaaa aacgcggtag gctaccaaag tgcaaaagat ggagttgcgt
     2521 atgctggctt ggttccctat agtaatttta gcgtannnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnaattt atatagtagc aattgaagat aaatatggga gaatggttta
     2701 taagaaattt gtgaagatgt aattgaaagt gaataatact tacacataaa gccaaaaaaa
     2761 aacctgctat ataaaagcag gttcttatat attttaaaaa aggtaatgag ctcaaaaata
     2821 gttggtggaa ttgtcaaaaa atagttatat aatgctcccg cgcatttgaa gagggaacaa
     2881 gctctgcggg agcgcaactt gtgagcaata aaatgtgtga aggctaatgc cagtaaaagg
     2941 tgagcagtaa tagctcacct ttttatttta atactggcag tgtcttctct atggctaaaa
     3001 aaactcattt gtttttcaaa ctaagatacc atttcaaaaa atttttgcga tgatttgccg
     3061 ataaccctct atgcacagta agatgatttt tgagatgtcc gaaaaatgat tcaaggccat
     3121 tggtggtttt aggaattcga ggattatcaa gatagtgaaa catgtctggt aatgcatttt
     3181 tgatagtcat aaatgagcgt ctgactaatt tatgcttgta ccaatatcgt tttgaagttg
     3241 gattaaaact tttctcattg ataaagagct cgtatttttc gtaccatcta accatgctaa
     3301 caatccaaca atctctttca aatgcggttt gtattagatg tagttgcgaa ataattattc
     3361 tgagttcttt gccggcatcg ctcaaaggcc ttttggtaag ccatattcgg cacatgcgtt
     3421 gaatatgaac caggcatcgc tgtaacgttg cgccattact acttctacgc acagctttta
     3481 aaatagcctt atgtccatcg caagtgatac tttcaatttt tactccaagt gcatggagat
     3541 tctctaaatc ttctttcaac tcatcatacc attctccatt actgatccga taaagctggg
     3601 tgaatttaat tgtgttatct cggtaaataa tcaaacataa atcattgctg aaatacgtgc
     3661 cgtcaatcat caggtttact ttttctgaag ggtaaacttt ccattgtggt ggcttgtcaa
     3721 ggtatttata aaaatatctt tttaaagtgc taatcgaata accactttct tgttgaattt
     3781 gttgtaatgt ctgcctaccg attatccatt tttcaaacca aataaaacgg tttgattcct
     3841 tgacagatgt gtttgtccaa gtgaataaca aaccacaagt tttacattta tatcgttgct
     3901 tgtgattctg ttttccccat ttaattacaa cagtgccttt acagcaccag catgactttt
     3961 tttatatgtt tccattaacg aaaaaaagga aattgaaacg actttcaatt tccttcccga
     4021 aaactctacg tacataagtc tttaagttac attctaccaa ctatttttga ccattatatc
     4081 taaaaaatat ttttttagct gaaaggtacg atagaaacgt aagatttttc gtcacgcttt
     4141 ttgcggaatt gtactttacc atcaaccaaa gcaaatatgg tatgatcctt acctaatcca
     4201 acattgatac cgggaaagtg cttagtgcca cgctgacgaa ctataatatt acctgcaatg
     4261 gctacttggc caccatatat ttttattcct agtctcttac tgtgtgattc gcgtccgttg
     4321 cgcgaactac cggctccttt tttgtgtgcc atcttttttt aagtttttag ttaatgttaa
     4381 aagtttgaag ttacatgctt gttgcttatt gaccattcac aattgaccat tgcctattga
     4441 tcattgccaa ctctctataa ttgaatattt gcaatttgga ttttagtcaa gtcttgacgg
     4501 tggccattgc acttttgata tccttttctg cgtttctttt taaagattaa taccttatcg
     4561 ccacgtaaat ggtttattac ttttgcagtt actctggctc ctgacaatgt aggcgcaccc
     4621 acagttatcg agccatcatt atctaccagt aaaactttgt caaattctac ttcggcacct
     4681 tcatcggcct ttaagcggtg tacaaaaacc gattggtctt tgctcacttt aaactgctgg
     4741 ccggcaatgt caactattgc gtacatgtta ttgttttgtt tgatttatcc ccaaaaatgg
     4801 gagcgcaaat gtaaataatg tttttattta tacaacactg catttaatta ttttgtgaaa
     4861 tacttattcc tcaattggcc tgccccgact taagtgcttt tggcttgggt ttcaaactga
     4921 tgagatatac gccccctaga ataactgcca ggccaatcat ctgcatttga tgcacacttt
     4981 cgccatacaa taccccaagg cttaaactca ctataggcat aaggtaagta accgaagctg
     5041 caaacaaagc cgatgaagct tttacaagca tattgaacag aaccgttgct aaagcactgc
     5101 ccataactcc cagcacagct acacttgcga atgccctcca aaaatagggt tgattaccca
     5161 cagtagtgaa aaaacctgaa cccaaaaaat ataagaaata aggtatagcc gcaaatgcga
     5221 gagcaaagcc cgctgcatgc acagggttca ttccgccaaa gtgattgcgg ataatattta
     5281 cgctgcaacc atatcctaag catgccaata ccacatacca ggcataaggg tctgcattta
     5341 ttccggtgct tccgggctga aagtagatca gtgccaatgc ccctatcatg ccaatcagca
     5401 cgccaatagt ttgagttatg cgtggtttgt ttttaaatat taaccatgct atcagcaatg
     5461 tgaaaacggg cgaaagagaa ttgagcatgc ctgctgttgt gcttgtaact cgggtttcgg
     5521 caagggtaaa caaaaatgcc ggaatgccat taccaataag tccgcctgca ataatccatt
     5581 tccattttga tggttctatt ttacctacat gccgtataat gaagggcaaa agagccgatg
     5641 cagcaaatac catacgcata cctgccactt gaattggtgt aaatgccata agcccttcct
     5701 tcatcaaaat gaacgaactc ccccaaatga gggtaagcaa aaaaagtaaa atccatttta
     5761 agtatttagg gaatctactg agcataattt ttaaaattaa tacaaaccga ccttaagttt
     5821 ggggcaaacc caataaatat gagccatttt ggggtgcaat agtaccactt tatttactgt
     5881 caccctctcg tttaactgaa ctttatatac catgaaaggc actttttaat tttcgatttc
     5941 gagtttttgg gttgaaatgt taattgttga aaaacttgtt aaaagttttg gactaaattt
     6001 ttgcataata aggttcaagt tttcgaaatt tatcacctca atatttattg taaaaatagc
     6061 ataaaagaaa aatcaaactt ctattgaag
//
LOCUS       DAORQA010000096         6080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3317_length_6080_cov_5.945560, whole genome shotgun sequence.
ACCESSION   DAORQA010000096 DAORQA010000000
VERSION     DAORQA010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3317_length_6080_cov_5.945560"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2537
                     /locus_tag="PK736_06060"
     CDS             <1..2537
                     /locus_tag="PK736_06060"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRC32989.1"
                     /translation="LNNTIQDVLIRRARMLGKNACWVPGTDHASIATEAKVVAMLKEQ
                     GINKSDLSREEFLKYAWEWKEKYGGIILNQLEKLGASCDWERTRFTMEDDLSEAVIDV
                     FIDLYRKGQIYRGVRMVNWDPQGLTAVSDEEVIRKEVMQKLYYVQYAVVDANEKFDTM
                     DAVAAQAKFAEGNFITIATVRPETILGDSAVCIHPDDERYKKFHGKHVAIPYTNRTVP
                     VICDEYVTMDFGTGALKVTPAHDVNDYNLGIKHKLEVINIFNENGTMHERAGKYIGED
                     RFAVRKKIAADLQADGYLIKTEDYKSQVGYSERTNAAIEPRLSMQWFLKMDKISQPAL
                     ENVLNGNIKLIPDKFLNTYKHWMENVHDWCISRQLWWGQRIPSWYNETGEFMVAKTEE
                     EARKLFEEYNSKLVAQGGKAVAYDKLQQDEDVLDTWFSSWLWPISVFDGFKDPNNADI
                     KYYYPTNDLITAPEILFFWVARMIIAGYEYRNEKPFTNVYLTGIVRDKLGRKMSKSLG
                     NSPDPLELITKYSADGVRVGMLFSSPAGNDLPFDEGLCEQGRNFANKIWNAFRLVNGW
                     TVSKDVAVSNVALAAIEWYKNKLSATVAELNELYDKYRISDVLLATYKLVWDDFCAWY
                     LELVKPEFINGEPQPISQIVYEETVLLFEQNLIVLHPFMPFITEELWHTIRKRDENDC
                     CIVATWPTVSVANEAILKQFEHAQQIVMALRNMRAAKQLSPKEALEFIYTNSNYAGNV
                     YEIVAKLANVKPVLSEKHNATQGTYTLMVDTSEYYIPLEGKTDTAADKKKAEEEIAYL
                     EGFLKSVDAKLNNEKFVSNAKPEVIENERKKRADAVSKMEMLKRIF"
     gene            complement(2570..4885)
                     /locus_tag="PK736_06065"
     CDS             complement(2570..4885)
                     /locus_tag="PK736_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRC32990.1"
                     /translation="MTNKANIRLFSILLVYTIITACNPLRQIPEGKYLLTNNQIKVDK
                     SEFKEKLAPVIKQKPNRKVLGLFRFHMSVYTWAAKHPTRRFNKWMMNTIGEEPVLIDT
                     LLMRKSSEQLLLYMKHQGYYDATIIDSVKYKRKKAQVYYNIKANQPYTIAKLDMKMQD
                     TSLQRLIESYSAESLILTGDVISQDVCNAEAERITKALKNIGYYAFSQSYIKYKADTA
                     FGNHTLKLLMMVSNEQSKDTSIQESKIHHQFFVDSVMIYVGFDPLMNDSIQFFTDTVY
                     TDGYYFLYKPGSRIFNTRILLRNTLIQPSALFNQSEVEQTYTRLADIGTFKFVNIKFI
                     NITPRDSLINNMTCNIFLTPIKPKSYNIDSEVNYNGGNVGLAGYLTFRNNNAFRNGEL
                     IEFALHGSVEATPAISSTATGEQKKIFLFNTFDLGPEINLSFRDFLFPFAKKLKKEKS
                     NIVTTIATKYNYEVRSEFKRSSANLSLGYNFRNLKKVSFLWSPITINLLDVNLSEAFE
                     NILLATGDKGLISSYQKQLIAGGAFSFVYSTPNAKSKKLLKYLRFNIETSGHTLYLFD
                     KISNNTLNIDNRYTRFNIPYTTYTRPDFDFRIYRFFKSSNSLVYRFVCGVGYTYWNST
                     RMPFDKSFFAGGSNDQRAWKPRTLGPGSFNNGDNFNRIGDIKINSNVEYRFDILKIIE
                     GAAFVDAGNIWNLKKDKDFPGAEITTKRFLDEIAIGTGLGVRFNFTFFIFRIDAGVRV
                     KDPTNPAGQTWVYQNIKPKNFLFNYGIAYPF"
     gene            4998..5762
                     /locus_tag="PK736_06070"
     CDS             4998..5762
                     /locus_tag="PK736_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRC32991.1"
                     /translation="MSITKNQISFVKELHKKKERQAQSLFIVEGEKCVHELLNSTFGI
                     ETIFATAEWIDANSVILMQKKLPYHEISPAALERISTMSQPNKVLAIAKQPHSAFEIN
                     ALVNKFALVIDGINDPGNLGTIIRVADWYGIENIICSPTTCEQYNPKVVQAAMGSLFR
                     VNCTYIDIVSFLADYKQKVNQPVYAAHMQGVSINDVTILPLGILVLGSESHGISTEVM
                     SMCDSKLTIVQKSIHQQTESLNAAVAAAIIINKILG"
     gene            complement(5796..>6080)
                     /locus_tag="PK736_06075"
     CDS             complement(5796..>6080)
                     /locus_tag="PK736_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC32992.1"
                     /translation="NKKIRFSLMQVTEADGNIIQLTKKEAALLRLLIDHKDEVVSREH
                     ILETVWGYDVYPSTRTIDNFIVTFRKYFEENPSNPRYFQSVRGVGYRYSK"
BASE COUNT         1918 a         1065 c         1320 g         1777 t
ORIGIN      
        1 tgcttaacaa cacgatacag gatgtgctta ttcgcagggc gcgcatgttg ggcaaaaatg
       61 cctgttgggt tcccggtacc gatcatgcat cgatagcaac tgaagcaaag gttgttgcaa
      121 tgttgaaaga gcagggaata aataagagcg atttgtcgcg cgaagaattt ttgaagtatg
      181 cctgggagtg gaaagaaaag tatggcggca ttattcttaa tcaattggaa aagcttggtg
      241 catcgtgcga ttgggagcgt acacgattta caatggagga tgatttgagc gaggcggtga
      301 ttgatgtgtt cattgatttg tatcgcaaag gacaaatata tcgaggggtg cgcatggtaa
      361 actgggatcc acagggattg actgctgtga gcgatgaaga ggtgattcgg aaagaagtaa
      421 tgcagaaatt gtattatgtg caatatgctg tggttgatgc caacgaaaaa tttgatacaa
      481 tggatgctgt ggctgcacaa gcgaaatttg cagaaggtaa ttttattacc attgcaacag
      541 tgcgccccga aactatattg ggcgatagtg cagtttgtat tcatcccgat gatgagcgtt
      601 acaaaaagtt tcatggcaaa catgttgcta ttccttacac aaaccgcact gtgcctgtaa
      661 tatgcgatga gtatgtgact atggattttg gaacaggtgc attgaaagtt acccctgcac
      721 acgatgtgaa cgactacaac cttggtataa aacacaagct ggaagtaata aatattttta
      781 atgaaaatgg tacaatgcac gagcgtgcag gcaagtacat tggtgaagac agatttgcag
      841 ttcgcaaaaa aatagctgcc gatttgcaag ccgatggtta ccttataaaa acggaagatt
      901 ataaaagtca ggtggggtac agcgagcgca ccaacgcagc catagagcca aggttgagta
      961 tgcaatggtt tttgaaaatg gataagattt cgcaaccggc acttgaaaat gtattgaacg
     1021 gaaatataaa acttataccc gacaagtttt tgaataccta caaacactgg atggaaaatg
     1081 tacacgactg gtgcatatcg cgacagcttt ggtggggcca acgtataccg tcatggtata
     1141 atgagacggg cgaatttatg gttgcaaaaa ccgaggagga agctcgtaaa ttattcgaag
     1201 aatataactc gaagctagta gcccaaggtg gtaaggccgt tgcttatgat aaattgcaac
     1261 aggacgaaga tgtgcttgat acatggtttt cgtcatggtt gtggcccata tcggtgtttg
     1321 atggttttaa ggatcccaac aatgctgata taaaatatta ttatcctaca aatgatttaa
     1381 taacagcacc ggagattttg tttttctggg tggcgcgtat gataatagca ggatatgaat
     1441 acagaaatga aaaacctttt accaacgtat atctaaccgg aatagtacgc gataagcttg
     1501 gccggaagat gagtaagtcg cttggcaatt cgcccgatcc gctggaattg attacaaagt
     1561 acagtgccga tggtgtgcgt gtgggtatgt tgtttagttc acctgccggc aacgatttac
     1621 catttgatga aggcttatgt gagcaaggcc gcaattttgc aaataaaata tggaatgcat
     1681 tcaggctggt aaatggatgg acagtaagta aagatgttgc tgtttcgaat gtggcattgg
     1741 ctgctataga atggtacaag aataaactga gtgcaaccgt tgcagagttg aatgaattat
     1801 atgataagta ccgtataagc gatgtgttgc ttgcaactta taaattagtg tgggacgatt
     1861 tctgtgcatg gtatttggag ttggtaaagc ccgaatttat aaatggtgag ccacaaccta
     1921 tatcacaaat agtatatgaa gaaactgttt tattgtttga acaaaattta attgtgttgc
     1981 atccatttat gccattcatt accgaagagt tatggcatac catacgtaag cgcgatgaaa
     2041 acgattgttg tattgtggca acatggccaa cagtaagcgt ggcaaatgag gcgattttaa
     2101 aacagtttga gcatgcccaa caaatagtaa tggcactacg aaacatgcgt gccgccaaac
     2161 aactgtcgcc aaaggaggca ttagaattta tttacaccaa tagcaactat gcaggcaatg
     2221 tatatgagat tgtagcaaag ttggccaatg taaaaccagt attaagtgaa aagcacaacg
     2281 caacacaagg tacgtataca cttatggttg atacatcgga atattatata ccactcgaag
     2341 gcaaaaccga taccgcagcc gataagaaaa aagcggaaga agaaatagcg tacctcgagg
     2401 ggtttttgaa atcggtagat gccaaattga acaatgagaa atttgtgtct aatgcaaaac
     2461 ccgaggtaat agaaaatgaa agaaagaagc gtgcagatgc cgttagcaaa atggaaatgt
     2521 tgaaacggat tttttaatgc gcccaccgtt acacgcgtca taacgcgccc taaaacggat
     2581 acgcaatacc gtaattgaac aggaaatttt ttggtttaat attttggtac acccacgttt
     2641 gtccggcagg attggttgga tcttttacac gtacaccggc atctatacga aaaataaaaa
     2701 atgtaaaatt gaaccgcaca ccaaggcctg tgccaatggc tatttcatcg agaaatcttt
     2761 tggtcgttat ttcggctccg ggaaaatctt tatccttttt caaattccag atgttgccgg
     2821 catcaacgaa tgcagcacct tcaattattt ttaatatatc gaagcggtat tccacgttgg
     2881 aatttatttt tatatcgcct atacggttaa agttgtcgcc attgttgaat gaaccgggcc
     2941 ctaaagtacg cggtttccat gcgcgctgat cattcgatcc tccggcaaaa aaacttttat
     3001 cgaatggcat gcgtgttgaa ttccagtatg tataaccaac accacaaaca aaccgataaa
     3061 ccaatgaatt gctgctttta aaaaatctat agattcggaa gtcaaaatcg ggacgagtgt
     3121 atgtggtata tggaatattg aaccttgtat acctattatc gatgtttaga gtgttgtttg
     3181 aaattttatc aaaaagatat aatgtgtgtc ccgatgtttc aatattaaaa cgtaaatatt
     3241 taagtagttt tttcgattta gcatttggcg tagaatatac aaagctaaaa gcacccccgg
     3301 caataagttg tttttgatag ctgcttatca atcctttatc tcctgttgct aataagatat
     3361 tttcaaaagc ttcagagaga tttacatcta aaagattgat agtaatggga ctccacaaaa
     3421 agctaacctt tttaagattg cgaaaattgt aacccagtga aagattggca gaggagcgtt
     3481 taaattctga acgtacttca taattgtatt ttgttgcaat ggttgttaca atattggatt
     3541 tttctttttt caattttttt gcaaatggaa atagaaaatc gcgaaagctg agattaattt
     3601 ccggacctaa gtcgaatgtg ttgaacaaga aaattttctt ttgctcacca gttgccgtgc
     3661 tcgatatggc aggtgtagct tcgacagaac catgtaaagc aaactcaatt aattcgccat
     3721 tacgaaatgc attgttgttg cggaaagtta aataacctgc caatccaacg ttaccaccat
     3781 tgtaatttac ttccgaatcg atgttatagc tcttgggttt tatgggcgtg agaaaaatat
     3841 tgcaagtcat attgttgatc aatgaatcgc gtggtgttat gttgatgaat ttaatattta
     3901 caaatttgaa tgtgcctata tcggccaggc gtgtatacgt ttgttctact tccgattgat
     3961 tgaataatgc agagggttgt attaatgtat tccttaataa tatgcgagta ttgaatatac
     4021 gtgaacccgg tttgtataga aaataatatc catcggtata aacggtatcg gtgaaaaatt
     4081 gtattgaatc gttcatcaaa ggatcgaaac caacgtatat catcaccgaa tcgacaaaaa
     4141 attgatgatg gattttactt tcctgaatac ttgtgtcttt gctttgttcg ttgctcacca
     4201 tcatcagcaa ttttaatgta tggttgccaa aggccgtatc ggctttgtac tttatatacg
     4261 actgcgaaaa ggcatagtag cctatatttt tgagcgcctt ggttatacgt tcggcttcgg
     4321 cattgcacac atcctgacta attacatcgc ctgtaagtat caacgattcg gccgagtaag
     4381 attcaatcaa ccgctgtaaa ctcgtgtctt gcattttcat gtcgagcttt gcaattgtat
     4441 aaggctggtt tgctttgatg ttgtaatata cctgtgcttt tttgcgcttg tactttaccg
     4501 aatcaataat ggtggcatca taatatccct gatgcttcat atacaaaagt aactgctccg
     4561 aagatttacg cataagcaaa gtgtctatta atacgggctc ttcgccaatg gtattcatca
     4621 tccatttgtt aaacctgcga gtaggatgct tggcggccca tgtatatacc gacatatgaa
     4681 acctgaaaag ccccagcact tttctgttgg gtttttgctt tattaccgga gcgagttttt
     4741 ctttaaattc agacttgtca actttaatct ggttgttggt gagcaaatat ttgccctcgg
     4801 gaatttgccg caaaggatta cacgctgtga tgatagtata caccagaagt atactaaaca
     4861 gccgaatatt tgctttattt gtcaccctat tgaaatacat tgttgcgcgt aaaaataatt
     4921 ttttctttcg tgttgcaata acggaaagca caatcaataa agtaaattat aaaatagcga
     4981 tgttaacatc aacataaatg agcataacaa agaaccaaat aagttttgta aaagaactgc
     5041 ataagaaaaa ggaaaggcag gcacaatcgc tgttcatagt ggaaggtgaa aaatgtgtgc
     5101 acgaattatt gaattcaaca tttggcattg aaaccatttt tgccacagca gaatggatag
     5161 atgctaacag cgtaattttg atgcagaaaa aattacctta tcacgaaatt agtccggccg
     5221 cgctcgaacg tatatctacc atgtcgcagc caaataaagt attggcaatt gcaaaacagc
     5281 cacattcagc gtttgaaata aatgcattag taaacaagtt tgctttggtt atagatggta
     5341 taaacgatcc gggcaacctc ggcactatta ttcgtgttgc cgattggtat ggaatcgaaa
     5401 atattatttg ttcgccaacc acatgcgagc agtataatcc aaaagttgtg caagcggcaa
     5461 tgggttcatt gttcagagtc aactgcacgt atatagatat agttagtttt ttggccgatt
     5521 ataaacaaaa agtaaatcaa ccagtttatg cagctcacat gcagggtgta tcgataaatg
     5581 acgtaacaat tttaccattg ggaatattgg tgctcggcag cgaatcgcat ggcatatcga
     5641 ccgaagtaat gtcaatgtgc gattccaaat taacaattgt gcagaaatcg attcatcaac
     5701 aaacggaatc gctcaatgcg gccgtggctg cggcaattat catcaacaaa atattaggat
     5761 aagccctgca agccaataat tttcaagaga aaattttatt tactataacg ataaccaaca
     5821 ccacgcaccg attgaaagta acgtggattg gaaggatttt cttcaaaata tttgcggaag
     5881 gttactataa aattatctat agtgcgggta gaaggataca catcgtatcc ccaaacagtt
     5941 tcgaggatat gttcgcgcga taccacttcg tccttatggt caattaacaa acgcaacagc
     6001 gctgcttcct tttttgtaag ttgaattatg ttgccatcgg cttcggttac ctgcatcagc
     6061 gagaatctga ttttcttatt
//
LOCUS       DAORQA010000097         5983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3386_length_5983_cov_31.811066, whole genome shotgun sequence.
ACCESSION   DAORQA010000097 DAORQA010000000
VERSION     DAORQA010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3386_length_5983_cov_31.811066"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PK736_06080"
     CDS             complement(<1..701)
                     /locus_tag="PK736_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32993.1"
                     /translation="MEAFINSEILQGLRKSEAIYHKVAELSHYIFNSDNLEGREKYME
                     ELEFLLQGIKGTDTHLTIFNHKTYAPELEVGHKEFWGPLPEGKMDERMVSILDLMDKD
                     YSKFPYESVEWFTKVLQQIPFDDKVNMKIHHSGMRFTRMDGKPISIFSQGMPIQVDEN
                     RNFKYTLNYVQNIHHLMKKEFAHYWIRFSYGNEKQNVQTFHSMDKLYSKNDLLSAREK
                     EILKLIAADTDTKEIA"
     gene            1053..1709
                     /locus_tag="PK736_06085"
     CDS             1053..1709
                     /locus_tag="PK736_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRC32994.1"
                     /translation="MITTNEFYSDKVTAVPIIGVIRKASKTIDTAALVQMIKAKDEKG
                     FNTLYDNYSSSLYGILLKIVRHNDVADDLLQDTFVKIWQHIDKFDAVKGTLFTWMLNI
                     ARNLAIDHQRSSSYRMQLQMISIDVPFFREPNTNTTTSYINEGEFKDFQKKAMQLEPK
                     YAEVIDLIFFYGWTQEQTAQLLKLPLGTVKTRARKGLSILKVLYQHRCLAGFTCDMFY
                     "
     gene            1753..1995
                     /locus_tag="PK736_06090"
     CDS             1753..1995
                     /locus_tag="PK736_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32995.1"
                     /translation="MTTTNNITSGILQDYCLGLLNQEEERKVEALCQQYPQLSKELNL
                     LRQALESYANSNKVSPPHELRNLVWKAVKKIWEENQ"
     gene            complement(2012..3205)
                     /locus_tag="PK736_06095"
     CDS             complement(2012..3205)
                     /locus_tag="PK736_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HRC32996.1"
                     /translation="MRIIITIIILSISLHGFGQSIRTIKPELGGSFDFTKAEEIIWQK
                     ALGQYAEINSGNRSYESLSAADKKMIDSLEQNGGPMTQGVGCSWYCGGGPYKITSSSY
                     LKEQGKASYLPENLHDFNLFTAWVPENETGVIGKKVNFHFKPFSPRVNQIIIYNGYIK
                     NTDLWQANVRAKKLKLYINNKPIANLELEDITAAQIFKIDPIQSTDSTKDLVLTFEVI
                     EIYRGTQYNDLAISEINFDGLDVHCFAAGTKILMADKSEKNIEDILTGDSVFTFNQTT
                     KKLEATQVPQLISTQHSNLAELTFDDRKIIATDDHPFMNEQMQWASLNPYKANKDYLQ
                     DKLVTQLFVGDCVFIPANCKFVQLRNIKYLKTTQITYTIEINSGDSFIANGLLVKTEK
                     VKLTD"
     gene            complement(3922..5376)
                     /locus_tag="PK736_06100"
     CDS             complement(3922..5376)
                     /locus_tag="PK736_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC32997.1"
                     /translation="MKRNLFCLLFCLIQSNLFAQIFFEKTYPDTAYSKGYGVQQMPDS
                     GYMILATTQVFTVDTVFLDSIFINKLFLIRTNSNGDTLWTKLLNDTSFNYLTGYEALD
                     LQKTSDGNLIALGGIGCCSGGVLVKFDYFGNIYWATAFSGPDINTLIFGMKEYFDKGF
                     LVTGSNGHLSNPNYYWDVYLVKIDSLGVIEFDTTYGEARSDEGRDIEFDLDSNTYIVS
                     KSWSFPLEATPTTYLIKLDKNFNKLWYKTYGTPTSGYYNQSMEKTADNNFVILAPDYG
                     FTHKELHIIKVDTAGNTIWNKVILDSIMLWEHSTIYTTLDDGFIVAGEHGFSITNWDG
                     YLIKLDSSGNIQWNYFFDVDSLSGFFNVIQTFDSGFAAIGYSSHQVPQESDVYLVKTN
                     KFGQLSSNPNNTVQISNETNSLMIFPNPATDKLYIWSQKNENIILSNLLGEIITERKL
                     SLVSNGNLVLDISMLASGIYLIKAGNEVRKFVKE"
BASE COUNT         1863 a         1083 c         1003 g         2034 t
ORIGIN      
        1 gcaatttctt tggtatcggt atcggccgca ataagtttta gtatttcttt ttcacgtgca
       61 ctcaacaaat cattcttcga atacagttta tccatcgaat gaaaagtttg tacattttgt
      121 ttttcattcc cgtacgaaaa tcttatccag taatgcgcaa attctttctt cataaggtga
      181 tgaatatttt gtacataatt caacgtgtat ttaaaattcc tgttttcatc aacctgtata
      241 ggcatgcctt gcgaaaaaat agaaatgggt tttccatcca ttcgtgtaaa cctcattcca
      301 ctgtgatgta tcttcatgtt taccttatcg tcaaaaggaa tttgctgtaa tacttttgta
      361 aaccattcta ccgattcgta aggaaatttc gaataatcct tgtccattaa atcgagaatc
      421 gaaaccattc tttcgtccat ttttccctcg ggtagtggtc cccaaaactc cttgtgacct
      481 acctcaagtt caggtgcata tgttttatga ttgaaaattg taagatgtgt atcggtgcct
      541 tttattccct gcaacaaaaa ttccagttcc tccatgtatt tctcccgccc ttcaaggtta
      601 tcactgttaa aaatataatg cgacagttcg gcaactttgt gatagatggc ttcacttttt
      661 cggagtccct gcagtatttc gctatttata aatgcttcca tttgatatat tttttttagc
      721 aatgattaat tatttttcaa aagtaaatta cgacttccat aaaagtacgt ttaagcaatt
      781 atttaatatt caggaatttc aaagtgtcat tttcaaatta ttatctctct caaaatgcta
      841 agaccaagct gaattacatt gaagaaaata taattttcca aattccttct ttcagtattt
      901 gttcaggagt ttattctaaa aacgcattcg aatttttttt aaagttcaaa actacttttc
      961 aaaatagtga tattcactat atacggtacc aatggttaag cataattttg tcgctgtaat
     1021 gtacacttct ttatcacact aacaattgat ttatgataac gacaaatgaa ttttatagcg
     1081 acaaagttac tgctgttccc ataattggag taatcaggaa agcctcaaaa acaattgaca
     1141 cagcagcgct tgtgcaaatg ataaaggcaa aagatgaaaa agggttcaac acactgtacg
     1201 acaattatag cagctcgttg tatggtattc tgctaaaaat cgtaaggcac aatgatgtgg
     1261 ccgatgattt gttgcaagat acctttgtaa aaatatggca gcacatcgat aagtttgacg
     1321 cggtaaaagg aacactattt acatggatgc taaatatagc ccgcaacctt gccatcgatc
     1381 atcaacgttc ttcaagctac cgtatgcaat tgcaaatgat tagcatcgat gttccatttt
     1441 ttcgcgagcc aaacactaat acaactacat catacattaa cgaaggtgag tttaaagatt
     1501 ttcaaaaaaa ggcaatgcaa cttgaaccga aatacgcaga ggtgattgat ttaattttct
     1561 tttatggttg gacgcaagag caaactgcac agttgttgaa actaccttta ggtacggtga
     1621 agactcgtgc ccgaaaagga ctatcaattc taaaagttct gtatcagcat agatgtttag
     1681 caggttttac ttgtgatatg ttttactgaa aacagggcac gcatttttaa acttaaacaa
     1741 cactatacaa caatgacaac tacaaacaat attacaagtg gaattttgca ggattactgc
     1801 cttggcctgc ttaaccaaga agaagaaaga aaggtagaag cattgtgtca gcaatatccg
     1861 caattgtcga aagaacttaa tctattgcgg caggcacttg aaagttatgc caacagtaac
     1921 aaagtttcac caccgcacga attacgaaat ttggtatgga aagccgtgaa aaagatatgg
     1981 gaagaaaatc aataagagca ttcgtttttt ttcaatctgt caactttact ttttcggttt
     2041 ttaccaaaag gccattggca ataaaactat cgcccgaatt tatttctatt gtataagtta
     2101 tttgagtagt cttcagatat ttaatatttc tcagctgtac gaatttgcaa ttggccggaa
     2161 taaaaacaca atcaccaacg aataattgtg taaccaattt atcctgcagg taatccttgt
     2221 ttgccttgta aggattcaat gaagcccatt gcatttgctc attcataaag ggatgatcat
     2281 ctgttgcaat aatttttctg tcatcgaatg taagttctgc aagattggaa tgctgcgttg
     2341 atataagttg cggtacctga gttgcttcca gcttctttgt tgtttggtta aaagtaaaaa
     2401 cgctgtcgcc tgttagaata tcttcaatat ttttttctga tttgtcggcc attaaaattt
     2461 ttgtccccgc agcaaagcaa tgcacatcga gtccatcgaa atttatttca cttattgcaa
     2521 ggtcgttata ttgtgtgcca cggtatattt ctatcacttc aaaagtcaac accaaatcct
     2581 ttgtgctatc ggtagattga atggggtcaa ttttaaaaat ttgcgcagcc gttatatctt
     2641 caagttccag gttagcaatt ggtttattgt tgatgtatag tttcagtttt tttgctctta
     2701 cattagcctg ccacaaatcg gtgtttttga tgtaaccgtt gtaaataatt atttgattta
     2761 ctctgggcga aaatggcttg aaatggaaat ttactttttt gccaatcaca ccggtttcat
     2821 tttccggaac ccatgcagta aataaattga agtcgtgaag attttcggga agataagatg
     2881 ctttgccttg ttcttttaaa taagacgaag aagtgatttt gtatgggcca ccaccacaat
     2941 accaactgca accaacgccc tgtgtcattg gcccgccatt ttgttccaac gagtcaatca
     3001 tttttttgtc agcggccgat agcgactcgt aagacctgtt acccgaattt atttcggcat
     3061 attggcctaa agctttttgc cagattattt cttccgcttt tgtaaaatcg aaactgccac
     3121 caagttccgg ttttatagtc cgtatgcttt gtccaaaacc atgaaggctt attgacaaaa
     3181 ttattatggt aattattatt cgcattgttg ttattaaatt gacatcgact gtatgtaaaa
     3241 aaaatgttac cagataaatg taaactgaat ggagtaaaaa taaaaaatat aatgatgcag
     3301 ctgacatcta tttgcatggg ctactgtttg aagctcgcat agtgttgtgg aataagattt
     3361 attttttttt gacgggttat aatttatctc cttttctaaa aaaagaaatg cccgttgtgt
     3421 ttgtattttt ttgtttcgtc aaacattgtg tttcgtttat ttattattgt tgaaccgtgt
     3481 cattactttt ctatttctcc attattaatt ttggcaataa aatatctctt ttgcaaactt
     3541 cgttggagtg tcggcttttg cggtttgcaa atgtgctgtt ttgtgagttg gaatttttct
     3601 tttatttatt tgtttttatg cggcaaaaat ttctttgtca ttgtgtggtt gtgttttttt
     3661 agtctggacg aaaatgtttg tgggtttaag aagttggcga ttatttatca ctaaagttgt
     3721 tgaggagtag ataactttct ataacgaaga agggattaaa gcagtaaaaa aaagaaggga
     3781 ttagaagcac tactgtttgt ttacctatac tgttctttag aaactcaact ctttgtttac
     3841 ctctcaaagc ccttcttttt ttattttttt taatctaaat gttatcggaa tacccagact
     3901 acgtttacga atttgttcag tttattcttt aacaaacttt cgtacttcat tgcctgcttt
     3961 aattaaataa atgcccgatg ccagcattga aatatctaat accaaattgc catttgatac
     4021 taacgaaagt tttctttccg taatgatttc accaagcaaa ttagatagaa ttatgttttc
     4081 atttttctgc gaccaaatat aaagtttgtc agttgcagga tttggaaata tcattaagct
     4141 attcgtttca tttgaaatct gaacggtgtt attcggattg gaacttaatt gaccaaactt
     4201 gtttgttttc accaaataaa cgtctgattc ttgcgggact tgatggcttg aatatcctat
     4261 tgcggcaaac cctgaatcaa aagtttgtat gacattaaaa aagccactca atgaatccac
     4321 atcaaaaaaa taattccatt gtatgttgcc actggaatcc aattttataa gatagccatc
     4381 ccaattagta atactgaaac catgttcccc tgccacaata aaaccatcat caagtgttgt
     4441 ataaatagta gaatgctccc aaagcatgat actgtcaagt ataactttgt tccatattgt
     4501 attcccggca gtatcaactt ttatgatatg taattctttg tgagtaaatc cgtaatcagg
     4561 tgcaagaatt acaaagttat tatcggcagt tttctccatt gattgattat aataaccaga
     4621 agttggagtt ccgtacgttt tataccatag cttgttaaag tttttatcaa gttttattaa
     4681 ataagttgtt ggagtagctt ctaaaggaaa gctccaactt ttggaaacta tataggtatt
     4741 actgtctaaa tcgaattcaa tatctcttcc ctcatcactt ctggcttccc catatgtcgt
     4801 atcgaattca ataacaccca aactatctat tttaacaaga tagacatccc aataataatt
     4861 tggatttgat aagtgtccgt ttgaaccagt gacaagaaat cccttgtcga aatattcttt
     4921 cattccaaat atcaaagtat taatatcagg tccgctaaaa gcggtagccc aataaatgtt
     4981 tccaaaataa tcaaatttaa caagcacacc tccagaacaa cagccaatac ctcccaatgc
     5041 aattaaatta ccatctgaag ttttctgaag gtcaagcgct tcataaccgg ttaaataatt
     5101 aaatgaagtg tcattaagca attttgtcca aagcgtatct ccatttgaat ttgtcctgat
     5161 taaaaaaagt ttgttaataa aaatgctgtc aaggaagaca gtgtcaacag taaaaacttg
     5221 agttgttgct aatatcatgt aaccactatc aggcatttgt tgaacaccat atccctttga
     5281 ataagcagtg tcaggatacg ttttttcaaa aaaaatctgt gcaaacaagt tagattgaat
     5341 cagacaaaat agcaagcaaa ataaatttct tttcatactg tttgaattag cactgccgtt
     5401 tcttctaagc gcaatgggtt tccgctaacg tcagactgtg aaataaccaa cttgcccaca
     5461 gctgcacgtt aacaacatgc ggtaaaaata aaaattacaa cgacactgct gacgcaactt
     5521 caaaaaaaat aaatatacat cacatagctg agggctgccg gcatcaaatg gaaatatcaa
     5581 gtttagattg caacatcata ctgaaaattt tgtacgcttt atttatgctt ttgtttgtaa
     5641 actcgattgc gtgaaattga aattggaaag aggaattgtt ggcgttgtat tttaaactac
     5701 aagaggctaa atcgtatttg gtcattgtta gaaagccaca aaaacaaaaa ttgctttact
     5761 attacaccta agcaatcccc cacattgaac tccgcaccac acccaaagtg tcgctgaaca
     5821 ccccattgga gtatacgggg caggcctaat attcaaaacc ccctcgttta ctacttgcac
     5881 gggggcttcg tcaatactgc aatgtgtttg tggcagtttt tttattcatt cattctgatt
     5941 ttcaaatcca tcctttcgtg caaaattctg ataacttcaa ttt
//
LOCUS       DAORQA010000098         5967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3392_length_5967_cov_21.530108, whole genome shotgun sequence.
ACCESSION   DAORQA010000098 DAORQA010000000
VERSION     DAORQA010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3392_length_5967_cov_21.530108"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..678)
                     /locus_tag="PK736_06105"
     CDS             complement(67..678)
                     /locus_tag="PK736_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC32998.1"
                     /translation="MTNTNDIFFDKELRTGGFYELAIQVCKSADNIPIRLYSDFFWTQ
                     KNVEGPFDSNKTSIVFDIKNSNHHGILHLDNFTIPFKTYNIREEEPIETGFYWFDICF
                     YTTTIEKVFSLTQQDWTDYPSVPNELKKFFMTTLAKLDKIFPLQLAILDFEASGQFYL
                     EDLKTPLTFIYNPPCFFVGQDNYEHISIENRKYVTKIEDITNE"
     gene            complement(941..1522)
                     /locus_tag="PK736_06110"
     CDS             complement(941..1522)
                     /locus_tag="PK736_06110"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRC32999.1"
                     /translation="MYCYNGLNFTRFLYLDGVTNNDSLKLKMVASMVEDNDGNIWFAS
                     GMPPGMEGVCRYDGKAITSYKPNGDGWIRYITKDKKGHLWFGGRSHGNFIYDGKNFTN
                     FTQKIGIGNCILSDKSGNIWFTGEEKISTIESASGIWCYDGKTFKNFNTKDGIGKYFV
                     HCLIEDRNGNIWIGTRNTGLYKYDGKTFTCFSE"
     gene            complement(1613..2203)
                     /locus_tag="PK736_06115"
     CDS             complement(1613..2203)
                     /locus_tag="PK736_06115"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRC33000.1"
                     /translation="MKKYFLSKLTTTLLAISIFANACNGQVKTNQTAKMVSEQKIMPV
                     GQPKLINTQGTQQNDNVHCGLQDKAGNLWFGTTGSGVYRYDGKLFYNYTTKDSLINNC
                     VWCMLEDKAGNIWFGTTDGICRFDGKSFTSFPINDFIRPANGNNNYYTKQSIKNSVWS
                     MLQDKTGTIWFGNGDGQCHYVKPSAHPESLKGASPL"
     gene            complement(2370..3446)
                     /locus_tag="PK736_06120"
     CDS             complement(2370..3446)
                     /locus_tag="PK736_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRC33001.1"
                     /translation="MEIPRIPVTIQSNKKYSILEILQFTVLTSIVLYFGRTLFIPLSF
                     AMLISFILYPLCKGMEKKGINKGISILVLMLLVAIMFCVIIYLLFTQLMQFSHEWQTL
                     KLKLLETKTQLSVFLVERIGVSLERQLEFIENFIGNLGSQIIPFLQNTAYSFSESIFL
                     LILILIFSSLILFHRRLLSNVIYQLFPTGNKMAIHEILIETIHAYYNFIKGMILVYLI
                     VGILNSIGLAIIGIPHPVLFGFIASILTFIPYVGIMIAALLPITFAWIAFDSIWYPVA
                     VIFVFAIVQLAEAYIIFPFAVGSRLKINSLVLIVTIVAGGILWGAAGMILFIPFVSII
                     KLIADRTESLKTISMMLGDGKNNK"
     gene            complement(3551..4294)
                     /locus_tag="PK736_06125"
     CDS             complement(3551..4294)
                     /locus_tag="PK736_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015361113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33002.1"
                     /translation="MKKKDKLAIIRQHYPNANTTIDSVNNFIDFVESQLDLEPSQVML
                     ADSICSDDVNSIQYPVRTQEFLGPFKMGGLDGFPFTGLTGMGAFASHVPDEGAVAIYY
                     GPHIGITKDGTIGEIHRHGQAKNSGCCGAAKGALNKLTNKLITEGKVTELDYQMNTIE
                     QILFNGSERILSAKFPLKEATEVIYEAIDKRMEELVAGTKYNCKFLILLGAIHINSDS
                     DVGSFNEARKFIVIDLKTGRRTNYLEKFL"
     gene            complement(4331..5317)
                     /locus_tag="PK736_06130"
     CDS             complement(4331..5317)
                     /locus_tag="PK736_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HRC33003.1"
                     /translation="MLINKKLTFEGIWLFARKNILQTFALSIIVVMLYQFLGLKIIAI
                     PFLPVATIGTAVAFYVGFKNNQAYDRLWEARRLWGGITNTSRILAASYISLVDDKTKQ
                     KDFLNRHIAYINILRLQLRKTIPWATNREDYHKQYVSASEEIKEFDIAIEALFEKLDK
                     KEIFEKVKHKGNIASYTLKYQINQITKLKREKIIDEYEHSDLTKLLAELYNLQGGCER
                     IKNTPLFRQYSLFSRLFVQLFIFLLPFALLTEMGKLGEHGIWLVVPFAVLISWVFMTM
                     EQIGESSENPFDNGAADTPISTIVRNIEIDILEMLDETNLPPKIEPFNDVLL"
BASE COUNT         1827 a         1104 c         1010 g         2026 t
ORIGIN      
        1 ccctcgtttg ctacgatgcc ggggggcttt cgtctatacg gctaaatgtt atgcgttcgc
       61 cattctttat tcattggtga tgtcctcaat tttagttaca tattttctgt tttctattga
      121 tatatgttcg tagttgtctt gtcctacaaa aaaacatggt ggattataaa tgaatgtcaa
      181 tggtgttttt aaatcctcta aataaaattg tcccgatgct tcaaagtcga gaattgcaag
      241 ttgtaaagga aatattttat ccagctttgc taatgttgtc ataaagaatt ttttcaactc
      301 gttaggaact gatgggtagt cagtccaatc ttgctgtgtt aaactaaata ccttttctat
      361 agtcgttgta taaaagcata tgtcaaacca ataaaatcct gtttcaattg gctcttcttc
      421 tcgaatatta taggtcttga aaggaattgt aaaattgtca agatgtaaaa taccatgatg
      481 gtttgaattt tttatgtcaa atactattga agtcttattt gagtcaaatg gtccttcaac
      541 gtttttttgt gtccaaaaaa agtcactata aagtcttatt ggtatattgt ctgcactctt
      601 gcaaacttga attgcaagtt cgtaaaaacc acctgtccga agttctttgt caaaaaatat
      661 gtcgttagta ttagtcattt aaagtctgtt gttgaggttc tatatggtga cgcataactt
      721 gcgcataccc gctacatcat agcgcatata caacactatt ctgcggatat gcgctacttt
      781 ttgttttatt cacaatgcaa caatacacat aaatcttgaa actcattcaa acaaaaggta
      841 tcattttaaa aagtatagat atacacaatt acgcatttcg ctttggtttt tttgtgaaag
      901 aatttggcag gttttgataa gttatttttg taagtatttt ttattctgaa aagcaagtaa
      961 atgtttttcc atcgtattta tataagccgg tgtttcgggt gcctatccaa atatttccat
     1021 ttctatcttc tattaaacag tgtacaaaat attttccaat gccatctttg gtgttaaaat
     1081 ttttaaatgt ttttccatcg tagcaccaaa tgccactggc actttctatg gttgaaattt
     1141 tttcttcacc ggtaaaccaa atgtttcccg atttatcgga tagtatacaa ttaccaatgc
     1201 caattttttg ggtaaagttt gtaaagtttt ttccatcgta aataaaattt ccatggcttc
     1261 ggccaccaaa ccataagtgt ccttttttat ctttggtaat gtagcgtatc catccatcgc
     1321 cattgggttt atagctagta atagcctttc catcatatcg gcaaacgcct tccataccgg
     1381 gtggcatgcc cgatgcaaac caaatgtttc catcattatc ttctaccata ctagcaacca
     1441 tttttagttt aaggctatcg ttatttgtca caccatcgag gtataagaaa cgtgtaaagt
     1501 ttaatccatt ataacagtac actccatcac cgcaatgtca ttaagttaag gaattaaatc
     1561 caattgcctg cgctcacccg tagccctaaa gggcgcctcc acgcaggttt cgttacaacg
     1621 gactggctcc ctttagggat tcagggtgcg cggagggctt aacataatga cattgcccat
     1681 caccgttgcc aaaccaaatt gttccggttt tatcttgcag catactccac accgaatttt
     1741 taatcgactg ttttgtatag taattgttgt tgccatttgc aggtcttata aaatcgttaa
     1801 taggaaagct tgtaaaactc ttaccatcaa accggcatat accatcggtg gtgccaaacc
     1861 aaatgttgcc tgctttgtct tccagcatac accaaacaca gttgttgatc aagctatctt
     1921 ttgtggtata attataaaat aattttccat cgtagcgata cacgccactg cctgttgtgc
     1981 caaaccaaag gttacctgcc ttgtcttgca aaccacaatg cacattatcg ttttgttgtg
     2041 tgccttgcgt atttattaat ttcggttgtc ccacaggcat aattttttgt tcactcacca
     2101 ttttagcggt ttggtttgtt tttacttgcc cattgcaggc attcgcaaaa attgaaattg
     2161 ccaaaagcgt tgttgttagt ttcgatagaa aatatttttt cactattaaa agtattataa
     2221 aatgattaga gtggaggcgc gctattttct aattattatg ttttaataaa tctgttgaac
     2281 acagtttgtg atatttcaga atactattta gttgctatgt catcaagtaa atgattttac
     2341 ctgataatta attttgataa gagaagttac tatttgttat tcttaccatc gcccaacatc
     2401 atggatattg tttttaagct ttcggtgcga tcggctatta attttataat gcttacaaat
     2461 ggaataaata aaatcattcc tgctgctccc cacaaaatgc caccggcaac aattgttact
     2521 attaaaacga gcgaattgat ttttaatctg ctaccaacag caaatggaaa tattatatat
     2581 gcttcggcta actgaacaat tgcaaaaacg aaaattaccg caacaggata ccaaatggaa
     2641 tcgaaagcaa tccaagcaaa tgtgatgggt aaaagtgcgg ctatcataat acctacataa
     2701 ggaataaagg taagtatgga ggcaataaaa ccaaaaagaa caggatgggg gataccgatt
     2761 atagcaagcc caatactgtt taatattcct actattaaat aaaccagtat catacctttt
     2821 ataaaattat aataggcatg aattgtttca ataagaattt cgtgaattgc catcttgttg
     2881 cctgttggaa aaagttgata aataacattg gaaagcaatc tgcggtgaaa taatatgagc
     2941 gaagagaaaa tcagaattaa tataagcagg aaaatagatt cagaaaagga gtaagcagta
     3001 ttttgcaaaa aaggaataat ttgtgagcct aaattgccga taaaattttc aatgaattcc
     3061 agttgtcttt ctaaacttac gcctattcgc tcaactaaaa aaacgctcaa ttgagttttt
     3121 gtttccagaa gttttaattt taatgtttgc cattcgtgcg aaaattgcat caattgtgtg
     3181 aacagcaaat aaataattac gcaaaacata attgccacca acaacattaa caccaggatt
     3241 gaaataccct tgttgatacc tttcttttcc atgcccttgc atagaggata aagaataaaa
     3301 ctaatgagca tggcaaaact caaaggaata aaaagtgttc tgccaaaata aagcacaata
     3361 cttgtcaata ccgtaaactg caaaatttcg agaatagaat attttttatt cgactgtatt
     3421 gtaacaggta tacgtggaat ttccattgtt atagtttagt ttttcctttt ccttttaaac
     3481 tgatattcgg ctaagtatta acacaggcat ttattcatag gaataattta ttggcaactt
     3541 tttatttgct ttataaaaat ttctctaaat aatttgttct tctgccggtc ttcaaatcga
     3601 ttacaataaa ttttcgggct tcgttaaacg agcctacatc cgaatcacta ttaatatgaa
     3661 ttgctcctaa cagaatgaga aatttgcagt tgtattttgt gccggctacc aattcttcca
     3721 ttcgtttgtc tatggcttcg tatataacct ctgttgcttc tttaagtgga aattttgccg
     3781 ataaaattct ttcacttccg ttgaataata tttgttcgat ggtattcatt tgataatcca
     3841 gttcggtaac ttttccttcc gttattaatt tattcgtcag cttatttaaa gcaccttttg
     3901 ccgcaccaca acatccacta tttttagcct ggccatgtct gtgaatttcg cctatagttc
     3961 cgtcttttgt tataccaatg tgtggaccat aataaatggc cactgcacct tcgtccggaa
     4021 catgactggc aaaagctccc atccctgtga gtcctgtaaa tggaaagcca tctaaaccac
     4081 ccattttaaa agggcctaaa aactcttgtg ttcgcacagg atattgaatg ctgttaacat
     4141 catcgctaca aatgctatcg gccaacatca cctgcgaagg ttccaaatct aattggcttt
     4201 caacaaagtc aataaaatta ttcacactat cgatagttgt atttgcattt ggataatgct
     4261 ggcggatgat ggctaatttg tctttctttt tcatatattt atttcaaaaa ttaagtgctt
     4321 gtttctggct ctaaagtaat acatcattaa agggttctat tttaggagga agatttgttt
     4381 cgtccagcat ttctaaaata tctatttcta tatttcttac aatggttgag ataggagtgt
     4441 cggcagcacc attgtcaaag gggttttcgc tcgattcgcc aatttgttcc atggtcataa
     4501 acacccacga aataagaact gcaaaaggga caacaagcca gataccatgc tctcccaatt
     4561 tgcccatttc ggttaacagc gcaaagggca acagaaaaat aaacagttgc acaaatagtc
     4621 tgctaaacaa actatattga cgaaacaatg gtgtattttt tatacgttca catccacctt
     4681 gcagattata taattcagcc agcaatttgg taaggtcact gtgttcatac tcgtcaatta
     4741 ttttttcgcg ctttagtttt gtaatctgat taatctggta ttttaaagta tagctcgcaa
     4801 tgtttccttt atgttttact ttttcaaata tttctttctt atccagtttt tcaaataggg
     4861 cttcaatagc tatatcaaac tctttaattt cttccgaagc agaaacatac tgtttgtgat
     4921 agtcttctct attggttgcc cagggaatag ttttgcgtaa ttgtaaacgc agaatattga
     4981 tgtatgcaat gtggcgatta agaaagtctt tttgttttgt tttatcgtca acaagtgaaa
     5041 tataagaagc tgccaatatc cgactggtat ttgtaattcc accccacaac cgcctggctt
     5101 cccatagtct gtcgtatgcc tggttatttt taaagccaac gtaaaacgct acagcagttc
     5161 caatagttgc aaccggcaaa aacggaatgg caattatttt caaacctaaa aattggtaca
     5221 gcataactac aattatactc agggcaaatg tttgtaatat gttttttcgg gcgaacaacc
     5281 atataccctc aaaagtaagt ttcttattta ttagcatagt ttgcttttgt tttatttatt
     5341 tttttttagt gcacggcttt gtcattgtaa aaacaacata gtgtgataac tggattgacc
     5401 aaaactacac tttaataacc taaagtaaaa aaaagaattc ttggattgtg ttgatctagt
     5461 ttggtagaaa aaacataaac ccatcgcgta gctgctttca gcaaatagaa atatcaagtt
     5521 taggttgcaa catcgatgtg gcaaatgttg tgtgctttat tgatgcatta gtttgtaaac
     5581 tcgaattagg gaagttggaa tttgaaagag aaattcttcc cgcgtcagcc catcattgcc
     5641 gtccgattta tcagacggat taaatatttt tcattaagct ttagcaaaaa ttatgttgcc
     5701 acacctgccc cgaccgcagt gtcagggaat gcacgaatga attccgaata atttcttaac
     5761 acaaagacgc aaaggtacaa agttgttttt cgcgtcagca ttcattgccg tccgatttat
     5821 cggacggatt aaataatttg cattatgctt tagcaaaaat tatgttgcca cacctgcccc
     5881 gaccgcagtg tcagggaatg cacgaatgaa tgccgaataa tttcttaaca caaagacgca
     5941 aaggtacaaa gttgtttttc gcgtcag
//
LOCUS       DAORQA010000099         5902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3444_length_5902_cov_7.818368, whole genome shotgun sequence.
ACCESSION   DAORQA010000099 DAORQA010000000
VERSION     DAORQA010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3444_length_5902_cov_7.818368"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PK736_06135"
     CDS             complement(<1..447)
                     /locus_tag="PK736_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC33004.1"
                     /translation="MSDKTRILLVEDELHIAQPLKLNLELEGYKVHHVDNGKAALKTF
                     KQERFSLVVLDVMLPEMDGFSVCEAIRLDNSKVPILFLTAKASSTDKVTGLKLGADDY
                     LSKPFNLEEFLLRVSNLLKRSGGNFNNVAADGVRVGMLFSSPAGNDL"
     gene            complement(531..2912)
                     /locus_tag="PK736_06140"
     CDS             complement(531..2912)
                     /locus_tag="PK736_06140"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRC33005.1"
                     /translation="MKRPLAIILFIFGMFAGENAMAFHIVGGDFTARHKFGNTWEVTL
                     KLFRDCSNSQGAPFDPSITMGVFRKGTNQLELSVEMYLDSIKPLGLTGNACAPPPQVC
                     MEIGYYVQDVIIPNYSLGHYLIWERCCRNSSVVNLNNPQNTAMAFYLELPNPTLQNST
                     AVFTYPPLPYMCIDQPFTYNFSATDIDNDSLWYKLVTPLDGGHSDPTQPNPFSLGGGG
                     QTPVSAPYAPVAWAPGYSTSNIIDGNPTISLNSYTGIVTCTPLSLGLYAMAIVCEEFR
                     NGVKIGLTRREIEFLVIPCNQNATPNISPTQAPGSNSNYDLFAGDTLCFDIKFSDPDD
                     SLFVEVTGDCLPGGTAVAPFASAQIDSGLKNVNVNFCWKTTCDHISTTPYKVKYTVRD
                     NGCPLPKYKSFDFTITVKEPPNILPVNLLCMEFLNANTMQMFYGIINPQDSDYFKYFI
                     IYKSIAGGAFFAFDTIYNTALDNFIDTAATNYANINYCYFIKGYNNCDRENISDTVCT
                     EDRKNKTENYIRTVSVLGPNEVNVKWENFPDAYFSTLYLYRKENDFGSGANSYSLINT
                     LTQYTADSIIDVNTTTGYKSYCYYMINQNYCKNFSPESNDACTILLTGEAGTFLNSMK
                     WSEYKTWRGGVATYELLRAEGITAPFKPLVTLGPADYAYIDQQLDFTKGFYRYIIKAS
                     EGPNGLNEVSWSNEIPLTQIPQVFVPSAYTPNDDGVNEFWKAQATFVRDFSCVVYNRW
                     GQLVFSSNDIFRSWNGTYNGEAAPQGVYFYRIKYSGYDSTNQYEKTGSVTILR"
     gene            3018..4415
                     /gene="rlmD"
                     /locus_tag="PK736_06145"
     CDS             3018..4415
                     /gene="rlmD"
                     /locus_tag="PK736_06145"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRC33006.1"
                     /translation="MSELQLIENLHIHDIADDGMAVGRHENQVIFVKHAVPGDVIDVN
                     VFKKKKKYAHASIVKVVTPSVHRTDPFCTHFGTCGGCKWQYLDYPTQLFYKQKMVVDA
                     LQHIGGLSGFETNNIVASANTKHYRNKLEFTFSNKRWLTVSEMGNTDLLMHNALGFHV
                     PGKFDKVLDINECFLQDDKSNQIRLFVKDFCLTNNFSFFDIRQQTGLMRNLVIRNTTN
                     GEWMVVVVFFENDAEKINLLMNAIRTQFNFITSLQYIINPKKNDSYSDLTPVLFSGLP
                     HITEVMENLKFQISAKAFFQTNPVQAHALYNAVLKFADLQSHHIVYDLYTGTGTIANY
                     MAPHCKHVVGIEYVEEAVKDAQVNSAINNLSNTVFYAGDMRKILTEEIVQQHGTPDVI
                     ITDPPRGGMDAAVVAQLLKINAQRIVYVSCNAATQARDIALMSNHYTLQSIQPFDMFP
                     HTHHVENIALLIRKN"
     gene            4539..5105
                     /locus_tag="PK736_06150"
     CDS             4539..5105
                     /locus_tag="PK736_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRC33007.1"
                     /translation="MKNYFFIILFSLSVNGICAQDLSRHQKDSIINYYNVGYYSQALN
                     LKLDTVANPQLFAAADEWLGTTYHYGGDDMRGIDCSHFSAMLYNYGYGFMLGNSAHAI
                     HKSVKMLVAKQDLKEGDLVFFKIGRSNIISHVGVYLGNNKFVHATTQAGVILSDLSEP
                     YYKKWFVTGGRMHQFILNRKYITSSGIE"
     gene            5379..5873
                     /locus_tag="PK736_06155"
     CDS             5379..5873
                     /locus_tag="PK736_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRC33008.1"
                     /translation="MIQILNSTQIQQRIDRIAYQVLEDNANEHSIYIAGIVDTGYILA
                     QRIQAALEKICSIQIHLLQLKIDKLGQANQHIHLAVESSELKNKVIIIVDDVLNSGKT
                     LTYALRPFLDADVKKIRTILLIDRDHRRYPVHADFVGLRLSTTMKEHITVSLEAGEAK
                     VFIS"
BASE COUNT         1803 a         1124 c         1120 g         1855 t
ORIGIN      
        1 taaatcgttg ccggcaggtg aactaaacaa catacccaca cgcacaccat cggcagcaac
       61 attattgaaa tttccgcccg accttttaag taaattgctt acgcgcaata aaaattcttc
      121 gaggttaaac ggtttcgata aataatcatc ggcaccaagc ttaagtcccg taaccttatc
      181 ggttgaagat gctttagctg tgagaaataa aatgggtacc ttcgaattat ctaaacgtat
      241 tgcttcacaa acactgaatc cgtccatttc gggtaacata acatcaagca caacgaggct
      301 aaaacgctct tgcttaaatg tttttaaagc cgctttgcca ttatctacat gatgcacttt
      361 gtaaccttca agttcaagat ttaattttaa aggttgtgca atgtgcaact catcttctac
      421 taataaaatt ctcgttttgt cgctcatagt cttttgtttt ttgatggtgc taatataaca
      481 ggttgaaaat tgctaattgt cgtaagaatg taaaaaggaa ataaactata ctaacgtaaa
      541 atagttacac ttcctgtttt ttcatattga tttgtggagt cgtatcccga atattttatt
      601 ctgtaaaaat atactccctg tggtgcagcc tcaccattgt aagttccatt ccaggagcga
      661 aaaatgtcgt tcgaagaaaa aaccaattga ccccaacgat tgtacaccac gcaagaaaaa
      721 tcgcgaacaa atgtagcctg tgctttccaa aattcgttta caccatcatc gtttggcgta
      781 taggctgaag gcacaaaaac ctgtggtatt tgtgtaagcg gaatttcgtt cgaccaactt
      841 acttcgttca atccattcgg gccttcgctg gcttttataa tgtatctgta aaatccttta
      901 gtaaaatcca gttgttgatc gatatatgca taatctgcag ggcccaatgt gacgagtggt
      961 ttaaagggtg cagtaattcc ttcggcacgt aaaagctcat aagttgcaac accaccacgc
     1021 catgttttat attcgctcca cttcatcgaa tttaaaaatg tgccggcttc acctgtcagc
     1081 aaaatagtgc atgcatcatt actttcgggg ctgaagtttt tacaatagtt ttggtttatc
     1141 atatagtaac aatatgattt gtaaccagta gttgtgttta catctattat ggaatcggca
     1201 gtatattgtg tcaaggtgtt tatcaatgaa taagagtttg ctccactgcc aaaatcattt
     1261 tcttttcggt acaaatacaa tgtagaaaaa taggcatcag gaaaattttc ccactttaca
     1321 ttcacctcat ttgggcccaa caccgaaaca gtacgaatgt aattttcggt tttgtttttt
     1381 ctgtcttcgg tacatactgt atccgaaata ttttcacgat cgcaattatt ataaccttta
     1441 atgaagtagc agtaattaat atttgcatag ttggtggctg ctgtatcaat aaaattatcg
     1501 agcgcggtat tataaatagt atcgaacgca aaaaatgctc cgcccgctat gcttttgtaa
     1561 ataataaaat atttaaagta atcggaatcc tgcggattga tgataccata aaacatttgc
     1621 attgtattgg cattaagaaa ttccatgcac aataaattta caggcaaaat attgggtggc
     1681 tcttttacag taatggtaaa atcgaacgat ttgtattttg gcaacgggca tccattatcg
     1741 cgcacggtat attttacctt ataaggagtg gtcgaaatat gatcgcaagt ggttttccag
     1801 caaaaattta cattcacatt ttttaaaccg ctatcaattt gggctgaggc aaaaggtgct
     1861 acagcagtgc ctcccggcaa acaatcgcct gttacttcta caaagagtga atcgtcagga
     1921 tcgctaaatt ttatatcgaa acacaaagta tcacctgcaa ataaatcgta gttgctattg
     1981 cttcccggtg cttgtgtagg actaatattg ggggttgcgt tttggttgca tggaattact
     2041 aaaaattcta tttcgcgcct tgtgagtcca atttttactc cgttgcgaaa ttcctcgcaa
     2101 actatagcca tggcatataa acccaaagac aacggagtgc atgtaactat gccggtataa
     2161 ctgttgagcg aaatggtagg gttgccatct attatattac ttgtgctgta accgggcgcc
     2221 catgctacag gcgcataagg tgccgataca ggcgtttgcc caccgccacc caacgaaaag
     2281 ggatttggtt gggttgggtc cgagtggcca ccatcgagtg gtgttaccaa tttgtaccac
     2341 aacgaatcgt tatcgatatc cgttgcacta aaattataag tgaaaggttg atcgatgcac
     2401 atatatggca acggtggata agtaaaaaca gcagtagagt tttgtaatgt tggatttggc
     2461 aattctaggt aaaaagccat agccgtgttt tgtggattat ttaaattcac aacggaagag
     2521 tttcggcaac aacgttccca aattaaataa tgccccagtg aatagtttgg aattataaca
     2581 tcctgtacat agtatcctat ttccatacat acttgcggtg gtggtgcaca tgcattaccg
     2641 gtaagcccaa gtggctttat agagtcgaga tacatttcca cactcaactc tagctgattg
     2701 gttccttttc taaaaacccc cattgtaata gatggatcga aaggtgcacc ttggctattg
     2761 ctgcagtcgc gaaagagttt taatgttact tcccaggtgt tgccaaattt atgtcgtgca
     2821 gtaaaatcgc cacccacaat gtgaaaagcc atagcatttt cacctgcaaa cattccaaat
     2881 atgaatagaa ttatggctaa cggacgtttc attgacatgt tcaatagtga taataagcta
     2941 catttgcact tcaaaattac aaaacacaaa gcgattaaca aagttttatt tgtgttaagg
     3001 aaagaacgta ataattcatg agcgaattac aattaataga aaatttacac attcatgata
     3061 ttgccgatga tggaatggca gtgggtcggc acgaaaatca agtgatattt gtgaagcatg
     3121 ctgtgccggg cgatgtgatt gatgtaaacg tttttaaaaa gaaaaaaaaa tatgctcatg
     3181 ccagcatagt gaaagtggtg acgccttctg ttcatcgcac cgatcctttt tgcacacatt
     3241 ttggcacttg cggtggttgt aaatggcagt acctcgatta ccctacacaa ttattttaca
     3301 agcaaaaaat ggttgtggat gccttgcaac atattggagg cttgtcgggt ttcgaaacca
     3361 ataatattgt ggcatctgcc aatacaaaac attaccgtaa taaactggag tttacttttt
     3421 cgaacaaacg ttggcttaca gtgagcgaaa tgggtaatac cgatttgctg atgcacaatg
     3481 ctttgggatt tcatgtgccc ggcaaatttg ataaagtgtt ggatataaat gaatgctttt
     3541 tgcaagatga taaaagcaat caaatacgtt tgtttgtaaa agatttttgt ctgaccaaca
     3601 atttttcttt tttcgatata cgccagcaaa caggtttgat gcgcaatttg gttatccgca
     3661 acaccactaa tggcgagtgg atggtggtgg ttgttttttt tgaaaacgat gctgagaaaa
     3721 taaatttgtt gatgaacgct attcgcactc aattcaattt tatcacctcg ctgcaatata
     3781 ttatcaatcc caaaaaaaat gattcgtata gcgatttaac gcctgtgctt ttttcaggct
     3841 taccacatat cacagaggtg atggaaaatt taaaattcca gataagtgca aaggcatttt
     3901 ttcaaaccaa tccggtgcag gcacatgcac tgtataatgc cgttttgaag tttgccgatt
     3961 tgcaatctca tcatatagtt tatgatttat ataccggcac cggtaccatt gcaaattata
     4021 tggcgccaca ttgcaagcat gtagttggta tcgaatatgt ggaagaagct gttaaagatg
     4081 ctcaggtaaa ttcggcaata aataatttat cgaacaccgt tttttatgcc ggtgatatgc
     4141 gcaaaatttt gacagaagaa attgtgcagc aacatggcac gcccgatgtt attataaccg
     4201 accctcctcg tggtggaatg gatgctgctg tggttgccca attattaaaa ataaatgctc
     4261 aacgcattgt ttacgttagc tgcaatgctg ccacacaggc gcgcgatatt gcattgatga
     4321 gcaatcatta tactttgcaa agcattcagc ctttcgatat gtttccacat acacatcatg
     4381 tggaaaatat tgctttactt attcgcaaaa attaattgtg atactcaaat gcagtgcttg
     4441 acttttgaaa ttaaaatttt gacttttagc cagtcactct tagattgaaa cttttgactt
     4501 atgaatttat atctttttaa ttataaactt tttgacttat gaaaaattac ttttttatta
     4561 ttttattttc actctctgta aacggaattt gcgcacagga cttaagccga caccaaaaag
     4621 attccattat caattattat aacgttggat actattcgca agcgttaaat ttgaagttgg
     4681 atactgttgc caatccgcaa ttgtttgccg ctgccgatga gtggcttggc accacatacc
     4741 actatggtgg cgatgatatg cgtggaatag attgttcgca cttcagtgcc atgttgtata
     4801 attatggata cggctttatg ctcggaaact cggcacatgc tatacataaa tcggtaaaga
     4861 tgttggttgc caaacaagat ttaaaagaag gcgatttggt attttttaaa attggccgca
     4921 gcaatattat ttcgcatgtt ggtgtatacc ttggcaacaa taaatttgtt cacgccacca
     4981 cacaagccgg agttattctc agcgatttga gcgagcctta ttacaaaaaa tggtttgtaa
     5041 ccggtggccg tatgcatcaa ttcatactaa acagaaaata tattacctcc tcaggaatag
     5101 aataaacata acacaacaac ggatttgaaa agcactaccc tttaatgtct tttttagtgc
     5161 attccgtatt accacttttt ggaatgtatt cattacggtt aaatcaattg cttcattcaa
     5221 gattgcattc tttgcgaatt ttgcagtttc tttctttgcg ttctttccgg ttaaaaaatg
     5281 gagctactgg aatttgcatt cgcatctgct tcagcactta atctcttctc agtttaatgt
     5341 aataaaagat tttctttctt tgcattttaa ccacagctat gattcaaatt cttaacagca
     5401 cacaaattca gcagcgcatc gatcgcattg cttatcaggt attggaagat aatgctaacg
     5461 aacattccat atatattgca ggaattgttg atacaggtta catacttgcc caacgtatac
     5521 aggcagcgtt agaaaaaatc tgttctattc aaattcattt gctccaactt aaaattgata
     5581 aacttggaca ggccaatcag catatacatc tcgcggttga aagcagcgaa ctcaaaaata
     5641 aagtaatcat tattgtggac gatgtattaa attcaggcaa gacccttaca tatgctttac
     5701 gtccttttct tgatgccgat gtaaaaaaaa tacgtaccat attactcata gaccgcgacc
     5761 acaggcgcta tcccgttcat gccgatttcg ttggcctccg actttcaaca acaatgaaag
     5821 aacatattac agtttcactc gaagcagggg aggcgaaagt ttttatttct taaacgtgtt
     5881 ttcgttagtt aatagttaat ac
//
LOCUS       DAORQA010000100         5873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3464_length_5873_cov_8.587487, whole genome shotgun sequence.
ACCESSION   DAORQA010000100 DAORQA010000000
VERSION     DAORQA010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3464_length_5873_cov_8.587487"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..2574
                     /locus_tag="PK736_06160"
     CDS             229..2574
                     /locus_tag="PK736_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRC33009.1"
                     /translation="MSSYLNELNETQRAAVEQLEGPVMIIAGAGSGKTRVLTYRITHL
                     INSGIDPFNILALTFTNKAAREMKARIAKLSGGEARNLWMGTYHSVFARVLRVEAEKL
                     GFPSNFTIYDTDDAKSLVRTILKEQGLDDKIYKPSVVLNRISNAKNNLRNWQDYQEDI
                     EQVNHDTQTGKPKIGQIFEQYSKRCLKASAMDFDDLIYNMYALLMRYPEALYKYQNKF
                     KFIMVDEFQDTNFAQYTVVKKLAAKLENICVVGDDAQSIYAFRGANIQNILNFEKDYP
                     DLHTFKLEQNYRSTKVIVQAANEVIANNKKQLQKTVWTDNDDGDKIKVLRANSDNEEG
                     KIIANQIFNDKMNHQCAHSDFTILYRTNAQSRALEEALRKMNLPYRIYGGISFYARKE
                     IKDLLAYFRLTINSNDEEAFKRVINFPKRSIGGTSIDKMIVAADNYDCSLWDVVEHIH
                     DYCRDISASTRAAIQDFGAMIKSFSVMAKTKNAYEIASHIATHSGILKEFYADRTPEG
                     VSHFENLQELLNAIKEFTDTGVTGVPAPEIDAEIENPNEVQNAEPVLRTLDMFMQDIA
                     LLTDADKDGDKDVNKDTISLMTVHASKGLEFKTVFVAGLEEGLFPSQMSVEDREDLEE
                     ERRLFYVAITRAETRLYLSYALSRYKWGNLQMCEPSRFIEEIPQSNLSFMASPRNEQR
                     IQPPSQDSWNAIGKSQFRLKPSSTLPSNFKKVNSATKNPAFNDDLRLLQNGMEVEHER
                     FGKGKVISMEGEYPETKCMVFFHGTGQKQLLLKYAKLKIVS"
     gene            complement(2823..3986)
                     /locus_tag="PK736_06165"
     CDS             complement(2823..3986)
                     /locus_tag="PK736_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRC33010.1"
                     /translation="MENKNPLKILIKTFRGLEDTLADECKKLGAVNIETLTRAVSCEG
                     DMGTIYKLNYQCRTALRILVPLTSFRAEDEEQVYNNITHFEWKDIFDANQTFAVSSTL
                     VQSQLNHTHYVSLKAKDAIADYFRKVGGTRPDVDIENPDFLIQVHISKDYCTVYLDST
                     GENLYKRGWRVEGHVAPLNEVLASGIIQISGWNNIAPVVDFMCGSGTLLIEAAMLALQ
                     IPAGFYRKHYAFTKWKDFDATLWQTIKDAADNKIIERPLRMYGSDNDMRAVEDTLANV
                     NNVQLQEFIHVEKKNFMDCSKQFEVGKIFVNPPYDVRITLDDAVAFYKDLGNIFKRNY
                     AGWQAHVLAGNLEAAKFIGLKPHRKHLLYNGPIECKLLSFNLFEGKKYGNVSS"
     gene            complement(4014..>5028)
                     /gene="corA"
                     /locus_tag="PK736_06170"
     CDS             complement(4014..>5028)
                     /gene="corA"
                     /locus_tag="PK736_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRC33011.1"
                     /translation="LYTYSNIEFQEFLPKNIGEAKAIMKANDGNVHWLEIHGFGDLQM
                     FNDISNYFDIHPLELEDVINTYQRPKMEEHAGHLFIVTRGLFLNAEQSFINEQLSMFL
                     SNNFVITMQDSYEDLLESLRNRLRRGKSILRNSGSDFLMYSINDTVVDNYFPLLEKIG
                     DRLDDLEEEALNDASRNTLTKIQTIKRELIVFRRTAFAERDKANDMLRSHNEIIREET
                     KVYLRDTYDHVMQSLDLIDSYKEITTGLTDIYLSSISNRMNQIMKILAVISTIFIPLT
                     FIVGLYGMNFASIDPLTNKPMPLNMPELYSPYGYVGVLILMAVMTISMIIYFARKGWM
                     WKD"
     assembly_gap    5029..5040
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5258..5267
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5268..>5873
                     /locus_tag="PK736_06175"
     CDS             <5268..>5873
                     /locus_tag="PK736_06175"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC33012.1"
                     /translation="YDLLYAHRDDTEAHLFINYLVLLLKPAANARILDLGCGKGRHAL
                     YLHQLGYAVTGVDLSADNIAYCKQFETDNLEFHIHDMRRYFRINNFDCVFNLFTSFGY
                     FEKLHHIFLAAQSAAANLKPGGFLVLDYFNAHFVENNLQHDTIVCKEEVVFRISKKIE
                     DNFVVKTINVEDKNVTDIFYERVELLYPHHFEELLQKVNLT"
BASE COUNT         1778 a         1132 c         1150 g         1791 t
ORIGIN      
        1 gtgtaaggac ggttaaagaa atgatttttt tcataaaaaa atattctggt ttacggattt
       61 taaaggtggc gaatatactt tatgaaattg cccttgtcaa gttttatttt ctcattttca
      121 ttttgcaata tttttttgaa acgcatattg tgcaaaactt aaattaaacg cttgccatca
      181 atattttact tttggcaact ccaattttct aattgtacaa tatatatcat gtcatcatac
      241 ctcaacgaat taaatgaaac ccaacgtgcc gcagtagaac aactcgaagg gccggtgatg
      301 attattgccg gtgccggttc gggcaaaacc cgtgtgctta cttatcgcat aacacatctt
      361 atcaatagtg gaatagaccc ttttaatatt cttgcactca cgtttacaaa caaagccgca
      421 cgtgaaatga aagcccgaat tgcaaagctg agtggaggcg aggcgcgcaa tttatggatg
      481 ggaacatatc acagtgtgtt tgcgcgtgtg ttgcgtgtgg aagccgaaaa actagggttt
      541 ccatctaatt ttacaatata cgataccgat gatgctaaaa gtttggtgcg taccatactc
      601 aaagaacaag gcctcgatga taaaatttat aagccctcgg tggtgctaaa cagaatttcg
      661 aatgccaaaa ataatttgcg caactggcag gattatcagg aagatattga acaggtaaat
      721 cacgatacgc aaacgggtaa acctaaaata ggacaaatat ttgaacagta cagcaagcgt
      781 tgtttgaagg cttcggctat ggattttgac gaccttattt acaatatgta cgcgttgctg
      841 atgcgttacc ccgaagcctt gtataagtac cagaataaat ttaagtttat aatggtagat
      901 gagtttcagg atacaaattt tgcacaatat accgtagtga aaaaacttgc tgccaagctc
      961 gaaaatattt gtgtggtggg tgacgatgct cagagtattt atgcttttcg cggtgccaat
     1021 attcaaaaca tattgaactt cgaaaaagat tatcccgatt tgcatacttt taaacttgag
     1081 caaaattatc gctcaacaaa agtaattgtg caagctgcca atgaagtaat tgccaataat
     1141 aaaaagcaat tgcaaaaaac ggtgtggaca gataatgatg atggcgataa aataaaagtg
     1201 ttgcgggcaa acagcgataa cgaagaaggt aagataattg ccaatcagat ttttaacgat
     1261 aagatgaatc atcaatgtgc acacagcgat ttcactattt tgtatcgtac caatgcacag
     1321 tcgcgcgcac tcgaagaagc cttgcgcaaa atgaatttgc cttatcgcat ttatggcggc
     1381 atttcgtttt atgcccgaaa agaaattaaa gatttgttgg cttactttcg gctaactatt
     1441 aattcgaatg atgaagaagc atttaaacgc gtgataaatt ttcccaagcg cagtataggt
     1501 ggaacaagca tcgataaaat gattgtagct gccgataatt acgattgcag cctttgggat
     1561 gtggtagaac acatacacga ttattgtcgc gatatatctg catcgacacg cgcggcaatt
     1621 caggattttg gcgccatgat taaaagtttt agtgtaatgg ccaaaacaaa aaatgcttac
     1681 gaaattgcca gtcatattgc cacgcattcg ggcatactga aagagtttta tgccgaccgc
     1741 actcccgaag gtgtaagcca tttcgaaaat ttgcaggagt tgctaaatgc aataaaagaa
     1801 tttaccgata caggagttac cggtgtgccc gcacccgaaa tagatgcaga aattgaaaac
     1861 cccaatgaag tgcaaaatgc cgaacctgtt ttgcgcacac tcgatatgtt tatgcaggat
     1921 attgccttgc ttaccgatgc cgataaagac ggtgataaag atgtgaataa agacaccatc
     1981 agtttgatga ctgtacatgc atctaaaggt ttggagttta aaactgtttt tgttgccggc
     2041 ctcgaagagg gtttgttccc aagccaaatg tcggtagaag accgtgaaga tttggaagaa
     2101 gaacgcaggt tgttttatgt ggcaattacg cgtgccgaaa cgcgccttta tcttagctat
     2161 gctttgtcgc gctacaaatg gggcaacttg caaatgtgcg agcccagtcg gttcattgaa
     2221 gaaattccgc aaagcaatct ttcgtttatg gcctcaccac gaaacgaaca acgtatacaa
     2281 ccaccgtcac aagactcgtg gaatgctatt ggaaaaagtc agtttcgttt gaagccttct
     2341 tcaacattac catcaaattt taaaaaagta aacagcgcca caaaaaaccc tgcctttaac
     2401 gatgatttgc gccttttaca aaatggtatg gaagtagaac atgagcgatt tggcaaaggc
     2461 aaagtaatat cgatggaagg ggagtacccc gaaacaaaat gtatggtatt ttttcatggc
     2521 accggacaaa agcaattgtt attgaagtat gcgaaattga aaatagtatc ttaatgcttt
     2581 ttcttagttc ttcgttacgc ttgcgatgcg ccaagttctt agttcttcgt ttcgtttgcg
     2641 gtgtggattt ttaatttcag agtctctcat gcctttgaaa acaaaaaata aatgaggact
     2701 ctgaaattag ctcggccgac ccaaacttag aacgtggcta tcaatagcgt agctaagaac
     2761 gaagaacatg gctggtatta aacgtaacta agaacaaaga acatggctaa cagtaaacgg
     2821 aactaagaac ttacattccc atactttttc ccctcaaaca aattaaacga aagtaactta
     2881 cattcaatag ggccattgta cagcaagtgt ttgcggtggg gtttcaggcc tataaatttt
     2941 gcggcttcga ggttgcctgc taaaacatgt gcttgccatc cggcataatt gcgcttaaat
     3001 atatttccta aatctttgta aaaggcaaca gcatcgtcaa gcgttatacg cacatcgtac
     3061 ggtgggttga caaatatttt tcctacctca aattgcttgg agcaatccat aaaattcttt
     3121 ttctctacat gaataaattc ctgcaattgc acattattta cattggcaag tgtatcttct
     3181 acagcacgca tatcgttatc gctgccatac atgcgcaatg ggcgttcaat tattttattg
     3241 tcggcagcat cttttatggt ttgccataga gtagcatcga aatctttcca ttttgtaaat
     3301 gcataatgct tgcgataaaa ccctgcggga atttgtaatg caagcatggc ggcttcaatt
     3361 aataatgtac cgctgccaca cataaaatca acaacgggcg caatgttgtt ccagccgctt
     3421 atttgaataa tgccagaagc aagtacttcg ttcagcggtg ctacatggcc ttccacccgc
     3481 cacccgcgct tgtataaatt ttcacctgtg ctatcaaggt aaaccgtgca ataatcttta
     3541 cttatgtgca cctgaattaa gaaatcggga ttctctatat ctacatcggg gcgtgtacct
     3601 cccactttac gaaagtaatc ggctatggca tcttttgctt ttaacgatac atagtgtgta
     3661 tggttgagtt gcgattgcac aagtgtacta ctcactgcaa aagtttgatt ggcatcaaaa
     3721 atatctttcc actcgaagtg tgtaatatta ttgtaaacct gttcctcatc ttcggcacga
     3781 aacgatgtta acggtacaag tatacgtagt gccgtacggc attgataatt taatttgtaa
     3841 atagtgccca tatcgccttc gcacgaaact gcgcgcgtga gggtttctat atttacagca
     3901 ccaagttttt tacattcatc ggcaagggta tcttccaggc cacgaaaagt ttttattaat
     3961 atcttcaacg ggtttttatt ttccataatt tttttctttt ttaattttca aaatcaatct
     4021 ttccacatcc aacctttacg ggcaaaataa attatcatgc ttatagtcat cactgccatt
     4081 aagattaaca caccaacata tccgtatggt gaatacaact ccggcatatt caacggcata
     4141 ggcttgtttg taagaggatc gatgctggca aagttcattc catacaaacc aacaataaat
     4201 gtgaggggaa taaaaatggt agaaatcacg gcaagtattt tcatgatttg attcatgcgg
     4261 ttgcttatgc tcgacaaata aatatcggtt aagcccgtag ttatttcttt atagctatca
     4321 atcaaatcga gcgattgcat tacgtggtcg taagtatcgc gcaaatatac tttagtttct
     4381 tcgcgaataa tttcgttgtg gctgcgcagc atatcattgg ccttgtcgcg ttcggcaaaa
     4441 gctgttctgc gaaatacaat caactcgcgt tttatagttt gtatttttgt aagtgtatta
     4501 cgcgatgcat cgttcaatgc ttcttcctcc aaatcatcga ggcggtcgcc aattttttcg
     4561 agcagcggaa aataattatc aaccacagta tcgttaatgc tatacattaa aaagtcactg
     4621 ccactgtttc gcagtatact cttgcctctg cgtaatctgt ttcgcaacga ttccagcaaa
     4681 tcttcgtaac tatcctgcat ggttatcaca aaattgttac ttaaaaacat tgataattgc
     4741 tcgttgataa aagattgctc ggcattaaga aacagtccgc gcgtaacgat gaacaaatgc
     4801 ccggcatgtt cctccatctt tggccgctga taagtgttta ttacatcttc gagttctaga
     4861 ggatgaatat cgaaatagtt agatatatcg ttaaacattt gcaaatctcc aaaaccgtgt
     4921 atttccagcc aatgcacatt gccatcattc gctttcatta tcgcttttgc ttcgccaatg
     4981 ttctttggta ggaattcctg aaactcgata ttactatagg tatacaaann nnnnnnnnnn
     5041 cggccgcaat attaaaatag cccggtacac cgcctattgc agccttgcgg ttctgtgcat
     5101 tctttaaact tggtttcttt gttgcacgca atttttttga atttccttct gttgccattt
     5161 ttatgttgga agtttttctt ttcagtaaac taacatcact atatttggcg aattaacaaa
     5221 aatatttccg ctatgtggta caacaagtgg tttggtannn nnnnnnntta cgatttgctt
     5281 tatgcacacc gtgacgatac cgaagctcat ttatttatta attatctggt gctgttgctt
     5341 aagcctgctg ccaatgcacg cattctcgat ttgggttgcg gcaaaggccg gcacgcattg
     5401 tatttgcatc aattgggtta tgctgtcacc ggtgtcgact tatcggccga taatattgcc
     5461 tattgcaaac aatttgaaac cgataatttg gaatttcata ttcacgacat gcgcaggtat
     5521 tttcgcatta ataatttcga ttgtgttttt aatctattta caagttttgg ttatttcgaa
     5581 aaattgcatc atattttttt ggcagcgcaa tcggctgcgg caaatttaaa acccggagga
     5641 tttttagtat tggattattt caatgcccat tttgtagaaa ataatttaca acacgataca
     5701 atagtttgca aagaagaagt tgtgtttcgc atttcgaaaa aaattgaaga taactttgta
     5761 gtaaaaacaa taaatgttga agacaaaaat gtgacggata tattttacga acgggtggaa
     5821 cttctttacc cacatcactt tgaagaatta ttgcaaaaag taaatcttac tat
//
LOCUS       DAORQA010000101         5816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3511_length_5816_cov_7.968235, whole genome shotgun sequence.
ACCESSION   DAORQA010000101 DAORQA010000000
VERSION     DAORQA010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3511_length_5816_cov_7.968235"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PK736_06180"
     CDS             complement(<1..189)
                     /locus_tag="PK736_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HRC33013.1"
                     /translation="MKLKFLSIAIIIALSAQGLFAQTKPDSIAITNNLKQHIKYLASD
                     ELQGRETGTIGERKAYEYI"
     gene            complement(228..1043)
                     /locus_tag="PK736_06185"
     CDS             complement(228..1043)
                     /locus_tag="PK736_06185"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC33014.1"
                     /translation="MTFLKSFCIAILLIYLLVLLLVYIYQGKLIFHPSKLGLDYKFEL
                     GELDKEIFLKTDDGQTINAIHFCSNNKRVVLYFHGNAGVLNSWQNTSAHFDGMHTDLL
                     VIDYRGYGKSTGTPTEQGLYLDAEAAYNYLISNGYDKDSIIVYGRSLGSGVASYIAAK
                     FGCRKLILETPYTSMVDISKSIHPYFFPQYTLKHRFSNMRYMPQINADMLILHGTHDA
                     LIPVSHCKEIYEATRSHSKLVVIKNGEHNNLPTFDEYTNALREFLNNTITLYP"
     gene            1756..2493
                     /locus_tag="PK736_06190"
     CDS             1756..2493
                     /locus_tag="PK736_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRC33015.1"
                     /translation="MSLKEKIDTSKLPQHVAVIMDGNGRWAKKQGMMRVFGHQNGVKA
                     VRDTVTAAAELGIKFLTLYAFSTENWSRSRLEVNALMELLVKSLAAEAKTLMENSIKL
                     TMIGDINALPDGARTKLQETMQLTANNTRLTLVLALSYSSKWEITEAVRKIAAHTKEG
                     MINLNDINAELISKHLSTANMPDPELLIRTSGEYRISNFLLWQIAYAELYFTDKLWPE
                     FEKEDFYAAIIDYQNRDRRFGGAKEEN"
     gene            2550..5063
                     /gene="bamA"
                     /locus_tag="PK736_06195"
     CDS             2550..5063
                     /gene="bamA"
                     /locus_tag="PK736_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRC33016.1"
                     /translation="MKKIFAALFTVICFMAALQPLAAQTTIGGLDNFEVDFTNPKEYE
                     IGGVTVTGIKFLDENALKTIAGLNVGERVRVPGEKFSNAIENLWKQGVLTDVKIVVTN
                     IKGDNIFLELQLTEKPRLSYFTPKGVTKGQFEKVRDKIHVRSGDVVTDNLIKTTQYVI
                     EEHFIDKGFLNAKCNIETITDSLRPNQVVMIINVDRGRKVKINKIYIADNTALKDIKI
                     KRKMKDTKERHWYRLFKTSKFIEENLEKDKEAVIEKYHEKGFRDARINLDSVVKNNRK
                     SVDLYLTAHEGNTYYFRNVNWIGNTKYRSGQLDTILGIKKGDVFNQKQMDQSLYMSQS
                     GQDVQSLYMDDGYLFFQITPAEVSVENDSIDLEMRIYEGKQATINRVYITGNTKTHDR
                     VVLREVYTKPGQLFNRSDVIRTVRELAQLGYFDQEKLVPVPKPNPADGTVDIEYQVEE
                     RPSDQLELSGGYGGNQLVGTLGLSFTNFSLRNIFKGREYTPLPSGDGQKLAIRAQSSG
                     SFYQSYNLSFTEPWLGGRKPNSFTGSIFHTIRTNGKTRSDPQRTNFKLSGISFGLGKR
                     LKWPDNFFSLYHELSFNLFDVTTPDGQNSFFIFKNGKAQNISYSVTLSRNSVDAPIFP
                     RRGGSLSFIVAATPPFSTFRNIDYATATPEQKYKWIEYHKWKFNTTFFTKITGNLVMA
                     TRLNFGFLGLYNRTIGAAPFERFYVGGDGMSGFDFDGREIIAMRGYDNQAVTPQLNRS
                     TVGATIYNKYTLELRYPVSLNPSATIYGLAFAEAGNSFLNFKEYSPFSVKRSAGIGVR
                     IFLPMFGLLGLDYGYGFDAPVGQRSVSKGKLQFTIGQQF"
     gene            5206..>5816
                     /locus_tag="PK736_06200"
     CDS             5206..>5816
                     /locus_tag="PK736_06200"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRC33017.1"
                     /translation="MRKTILMVALAAFFITLSTAVQAQKFAFIDTQYILDNIPEYKAA
                     QQQLDQISANWQKEIEAKYAVIDKLYKDFQAEQILLTDEMKKKREQEIVDKEKEVKEI
                     QKAKFGYQGELFKRKQELVKPIQDKVYNAVKKLATDQSFAIIFDKSSDLTILYANPKY
                     DKSDEVLTNLGYKPGSKSATQGGEKTPGSGSPGGGDTPRIYKK"
BASE COUNT         1936 a         1073 c         1158 g         1649 t
ORIGIN      
        1 aatatattca taagctttgc gctcgccaat agtgccggtt tcgcggccct gcaactcatc
       61 cgatgccaga tacttaatat gctgcttcaa gttgttggta atggcaatac tatcaggctt
      121 tgtttgtgca aataaacctt gtgcacttaa cgcaattatg attgcgatgg ataaaaattt
      181 caatttcatt tttgtttaaa ttatgaggac atcaaaatta caatttatta aggataaaga
      241 gtaatggtat tatttaaaaa ttcgcgcaaa gcatttgtgt attcgtcaaa tgttggcaag
      301 ttattatgct caccattttt tataaccacc agtttagaat gacttctggt agcttcataa
      361 atttctttgc aatgcgaaac aggaatgagg gcatcgtgtg tgccatgtaa aattaacatg
      421 tcggcattta tctgaggcat ataacgcatg ttagaaaaac gatgctttaa agtatactgt
      481 ggaaaaaaat atggatgtat gcttttcgaa atatcaacca tagatgtata cggagtttcg
      541 aggattaatt ttcggcaacc aaattttgct gcaatgtatg aggctacacc actgccaagt
      601 gatcggccat aaacaataat gctgtcttta tcatatccat tcgatattaa atagttgtaa
      661 gctgcttcgg catcaagata caacccttgt tcggtagggg tgcctgtact tttgccataa
      721 ccacggtaat ctatcaccag caaatcggta tgcattccat cgaaatgtgc gctggtattt
      781 tgccacgaat ttaatacacc ggcattgcca tgaaaatata aaaccaccct tttattgttg
      841 ctgcaaaaat gaatggcatt tatggtttgg ccatcatcgg ttttcaaaaa tatttcttta
      901 tcgagttcgc ctaattcaaa tttataatca agcccaagtt tcgatggatg gaaaattaat
      961 ttaccctgat aaatgtatac caacaacaaa accagcaggt aaatcagcag aattgcaatg
     1021 cagaaagatt ttaaaaatgt cattcgataa aaaaacaaaa ctacaattaa tgtttcgaaa
     1081 attgcttata gcttaattgt tgcctgctta gttttcatgt tctattatta aaactataat
     1141 ggcataaatt gttctgcacc aatagtcttt gaggcattaa accatgcatt cttttggaaa
     1201 aatattttca caagattgat actttggttg caatcatcaa taaactttga aataaccaaa
     1261 ataaaaaaac caccttgctg aagggtggtt ttcaatcggg tataaaactt aggtagtatg
     1321 gtttttttta aaatttattc aaagaacaag aagtttacaa tgcttcactt ttataaaatc
     1381 attgtactta attggattgt tgcacttttc agagagccgt tttttgaatg cggtaaaaat
     1441 gcatttctaa attaatgccg gtaagagggt aaatgctaca attacgggca agggttttcc
     1501 cttgttttcg aagcgagttg tttcgctata atcactaaat ttttctgatg caggctcagc
     1561 caatctgagt aattgataat tgccacttca ttgttgttaa tgtgggatta tgacatttat
     1621 tttggttaca attatttaat aaatacggct taccatcagc ctaaataagc attttagagg
     1681 ctggtgttaa cgaatattaa aaattggtgc actttctaaa cattcgatta catttgcgct
     1741 cccaaaaaat attgcatgag tttgaaagaa aagatagata catccaagtt gccacaacat
     1801 gttgcagtga taatggacgg caacggacga tgggccaaaa aacaagggat gatgcgtgta
     1861 ttcgggcatc agaatggcgt aaaggctgtg cgcgatacag tgacggccgc tgccgagttg
     1921 ggcataaaat ttcttaccct atatgcattt tctaccgaaa actggtcacg ctcacgcctc
     1981 gaagtaaatg ccctgatgga actactggta aaatcgttgg cggccgaagc aaaaaccctt
     2041 atggaaaata gcataaagct tacaatgatt ggcgatataa atgcgttgcc cgatggagca
     2101 cgaacaaaat tacaggaaac catgcagctt acagccaata atacaagact tacactggtg
     2161 cttgcattaa gttacagctc caaatgggaa ataacagagg ctgttagaaa aatagccgca
     2221 cacaccaaag aaggaatgat aaacctgaat gatataaatg ccgaattaat tagcaagcat
     2281 ttatctacag ccaatatgcc cgatcccgaa ttgctcattc gtaccagtgg cgagtaccgc
     2341 atcagtaatt ttttgctgtg gcaaattgct tatgccgagt tgtattttac cgataagctg
     2401 tggcccgaat ttgaaaaaga agatttttat gctgctatta tcgattatca aaatcgtgat
     2461 cgtaggtttg gtggggctaa agaagaaaat taattacaca cgataattca aaatcattcg
     2521 ttatgaatgg ttcgtattaa taaaaaagaa tgaagaaaat atttgcagca ctctttacgg
     2581 taatatgttt tatggcggcc ctgcaaccct tagccgcaca aactactatt ggaggcttag
     2641 ataattttga agtagatttt accaatccaa aggaatatga aataggtggc gttactgtaa
     2701 ccggcataaa gtttttggac gaaaatgcgc tcaaaactat tgcgggcctc aatgtaggag
     2761 aacgtgtacg cgtaccaggc gaaaaattta gcaatgccat cgaaaacctc tggaaacaag
     2821 gcgtacttac cgatgtaaaa attgtggtta caaatattaa aggcgacaat atttttcttg
     2881 aattacaact taccgaaaag ccacgccttt cttactttac gccaaaaggt gttaccaaag
     2941 gtcagtttga aaaagtacgc gacaaaatac atgtacgcag tggcgatgtg gttaccgata
     3001 atttgataaa aactacacaa tatgtaatag aagaacattt tatcgataaa ggatttttga
     3061 atgccaaatg caatatcgaa accataaccg attctttgcg gcccaatcag gtggtgatga
     3121 ttataaatgt ggacagaggg cgaaaggtga aaatcaataa aatttacatt gcagataaca
     3181 cagcccttaa agacataaaa ataaaacgca aaatgaaaga taccaaagaa aggcactggt
     3241 atcgattatt taaaacctcg aagtttatag aagaaaattt agaaaaagat aaagaggctg
     3301 ttatagaaaa ataccacgaa aaaggatttc gcgatgcacg cattaattta gattctgtag
     3361 taaaaaacaa ccgcaaatct gtcgaccttt atcttaccgc acatgagggc aatacttatt
     3421 attttagaaa tgtaaactgg ataggaaata ccaaataccg tagcggccaa ttggatacaa
     3481 tactcggaat aaaaaagggc gatgtattca atcaaaagca gatggaccaa agtctgtata
     3541 tgagccaaag cgggcaagat gtacaatcac tttatatgga tgatggatat ttgttttttc
     3601 aaatcactcc ggccgaagtg agtgtagaaa acgattcgat agacctcgaa atgcgcatat
     3661 acgaaggcaa gcaagctacc atcaacagag tttatattac aggcaataca aaaacccacg
     3721 accgcgttgt gctgcgcgaa gtatacacca agccgggaca actgttcaat cgcagcgatg
     3781 taatacgcac cgtacgcgaa ttggcacagt tgggatactt cgatcaggaa aaacttgtac
     3841 cggtgcctaa gccaaaccct gcagatggaa ctgtggatat agagtatcag gttgaagagc
     3901 gcccatcgga tcagttagaa ctttcgggag gatatggcgg taatcaattg gtaggaacgc
     3961 tgggattatc gttcacaaat ttttcgttgc gcaatatttt taaaggcaga gaatatactc
     4021 cattaccatc gggcgatgga caaaaattgg ccatacgtgc acaatcgagc ggttcatttt
     4081 atcagtcgta caacttatcg tttaccgagc cctggttagg aggccgaaag cctaattcat
     4141 tcaccggttc tatttttcat accatacgta ccaatggaaa aacccgcagc gacccacagc
     4201 gtacaaattt caaattaagt ggaatttcgt ttggtcttgg taagcgatta aaatggcccg
     4261 ataatttctt ttcattgtat catgagttga gtttcaattt atttgatgtt accactcccg
     4321 atgggcaaaa ctcgtttttt atattcaaaa atggtaaggc tcaaaatatc agctactcgg
     4381 taacgctatc gcgcaattct gtcgatgccc caatatttcc aagacgtgga ggttcgctat
     4441 cgtttattgt ggcagcaaca ccaccattct caacctttcg caatattgat tatgccactg
     4501 ctactcccga acaaaaatat aaatggatag agtatcataa atggaaattc aatactacat
     4561 tttttacaaa aattaccggt aatctggtaa tggcaacccg cctcaatttt ggtttcttag
     4621 gcttgtataa ccgcacaata ggagcagctc ctttcgagcg gttttatgtg ggtggcgatg
     4681 gaatgagtgg tttcgatttc gatggccgcg aaataattgc catgcgtgga tatgataatc
     4741 aggctgtaac accccaattg aaccgcagta cggtgggtgc aaccatttac aataaataca
     4801 cgcttgagtt gcgctatccg gtttcgctca atcccagcgc tactatatat ggtttggcat
     4861 ttgccgaagc aggcaatagt ttcctcaatt ttaaagagta ttcgcctttc agtgtaaagc
     4921 gctctgcagg tataggtgtt cgcatatttt tgcccatgtt tggtttgctg ggcctcgatt
     4981 atggctatgg tttcgatgca ccggtagggc aaaggtcggt aagcaaaggc aagttgcaat
     5041 ttaccattgg gcagcagttt taatttgtat actttttaat atcttcgccc gcctcaaaaa
     5101 agcgttaacg taatattcac ggcaaggttc actttcactt cagttaatgg gtgtaagtag
     5161 cacacaattt gaatagctaa cttgaaatta taaatgataa ataaaatgag aaaaacaatt
     5221 ttaatggttg ccttggcagc cttcttcatt accctaagca cagcagtgca agcacaaaaa
     5281 ttcgctttta tcgatacgca atatattctc gataatattc cggaatataa agctgctcaa
     5341 caacagctcg atcaaatatc ggccaactgg caaaaagaaa tcgaagccaa atatgcggtg
     5401 attgataaac tgtacaaaga ttttcaggct gagcaaatat tgcttaccga tgaaatgaaa
     5461 aagaaacgtg agcaggaaat tgtagataaa gaaaaagaag ttaaagaaat acaaaaggcc
     5521 aaattcggct atcagggcga attgtttaaa cgtaagcaag aacttgtaaa acctatacaa
     5581 gataaggtgt acaatgcagt aaagaaactg gcaaccgacc aaagctttgc aattattttc
     5641 gacaaatcga gcgaccttac tatattgtat gcaaatccaa aatacgataa gagtgacgaa
     5701 gtacttacaa accttggata caaacccggc agcaaaagtg caacacaagg tggcgaaaaa
     5761 acccctggtt caggtagtcc cggtggtggc gatactccac gtatatacaa aaaata
//
LOCUS       DAORQA010000102         5668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3627_length_5668_cov_6.286656, whole genome shotgun sequence.
ACCESSION   DAORQA010000102 DAORQA010000000
VERSION     DAORQA010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3627_length_5668_cov_6.286656"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>560)
                     /locus_tag="PK736_06205"
     CDS             complement(<1..>560)
                     /locus_tag="PK736_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRC33018.1"
                     /translation="VGDYANALHTLKESETISKEIDDASVLADVYKYLAKTLSKTGRF
                     EQAFYYYEACDNLKDSIYDADEMITQKEMVNKYESTIKEEKIKLLSAEKALDTAEAQK
                     QKQLKNIYLAGASMLLLLALVLYNRYDIKRKATIKLEEQNVIVQKAKERAEKSEQFKQ
                     QFLANMSHEIRTPMNAILGMSRLLLD"
     assembly_gap    561..595
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            685..>978
                     /locus_tag="PK736_06210"
     CDS             685..>978
                     /locus_tag="PK736_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC33019.1"
                     /translation="MPNKNAQNGRQVLDLLSDDNSKNFDLILMDVQMPLMDGFETTNF
                     IRKNMKEPVSNIPIIGFTASVIRTDLDKCIDAGMNCYVAKPFQEEELITAILSL"
     assembly_gap    979..989
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <990..1309
                     /locus_tag="PK736_06215"
     CDS             <990..1309
                     /locus_tag="PK736_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33020.1"
                     /translation="DDAGKNSEIGNSNIESSQADYKNLFLQLVPPRLQVIQQALSTND
                     LQTIKNMVHLMQPQLLHAGLMEHADLFASFEHYNENTKREEWLESTKLFCEIVETKLK
                     QME"
     gene            1323..2066
                     /locus_tag="PK736_06220"
     CDS             1323..2066
                     /locus_tag="PK736_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC33021.1"
                     /translation="MEKIKTIIIDDDIFIHAQLREQLMLLFPKVQVVAVCENALHGIE
                     AIKSHRPDLIFLDIQMPGLSGFEMLDHVDRNNLKVIFITSYNQYAIEAIRYSAFDYLL
                     KPIKEEELKNAIYRFEQANDEISFKGKIENLLHNINSEKEAFQLIIPLRQGEKKLQIK
                     NIVRCEADSNYTHFFLSDKTKFTASKTLKECEEMLSQKEFLRVHKSHLINKSFIQLLK
                     NDNHVVLTDGSLIEVSRRKLQEVKNSILE"
     gene            complement(2401..3612)
                     /locus_tag="PK736_06225"
     CDS             complement(2401..3612)
                     /locus_tag="PK736_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HRC33022.1"
                     /translation="MKRLINFLLIVTLFNFLISCNERTKGNATTKQNTDTKKLVGGGC
                     DGCEIMYVGMPANIKSVDTSAGWAEKGQKLLVTGTVFKLGGKTPAPNVVIYYWQTDNN
                     GYYSPKQGLHEKAKRHGHIRGWVKTDENGKYSINTIKPAPYPHENIPAHIHTSIKEPN
                     IDNEYYIDEFVFDDDKYLTGEKRKALENRGGSGILRVLPSGDLQIAEHNIILGLNIPN
                     YPETNKPEKQSGLEIGEDNPSFIPYHAFGPDKGSRACPVCKYGRFHGIVYFVGNNPNW
                     NDIKQWLTFLEQESVTRSKYLKAYFVYGNENNYVKDTRQKELEKIGNELNLKNIALTF
                     VPSMSDTESEVNLNKINPNVENTFIIYKHQTIVEKFIDLKPTEENFKKISNTLDKTKG
                     EYFNLQETEHN"
     gene            complement(3987..4427)
                     /locus_tag="PK736_06230"
     CDS             complement(3987..4427)
                     /locus_tag="PK736_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRC33023.1"
                     /translation="MKKSRPIHPDFQFLLKYKDHELIDLFCDLRQYILDLYPDSNELI
                     YHTHALTAVFSISEKLSDAFCMLPIYTNHLNLGFNKGALLKDPNKLLTGTGNLIRHID
                     VKKSNDYRNPKVKALIIEAIDFAIKDMDKPTKSIGATISKIKKK"
     gene            complement(4424..4807)
                     /locus_tag="PK736_06235"
     CDS             complement(4424..4807)
                     /locus_tag="PK736_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC33024.1"
                     /translation="MKNKLTHFAIHIDDIERAKNFYDKVFDWGFNSYGQDDFIQIKAD
                     KSENGELIGALQSRKYSPVADKIIGLECTIGVESIDAIIEKVKINGGQILMPKTAIPY
                     VGWIAKFLDTEGNLICGMQYNNTAR"
     gene            complement(4966..5121)
                     /locus_tag="PK736_06240"
     CDS             complement(4966..5121)
                     /locus_tag="PK736_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33025.1"
                     /translation="MAHCIIISGENHQLIKATNRKDVKKPFFKNIVDPKAFITVTLYG
                     FTTCYIR"
     gene            complement(5183..>5668)
                     /locus_tag="PK736_06245"
     CDS             complement(5183..>5668)
                     /locus_tag="PK736_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33026.1"
                     /translation="DTYDIYSQLIADYGIALVETQSYKKAIPSINKAIDLIKSNKKFT
                     ADTLPNVKFYETLIFYRGLCNYYLGHYELAQPDFELLVKLYPDNDIYPKWTNSIGNRK
                     LNRLKNISWYFVAGSVLIESFFSKTKLIKDITLGLGLLALLSAIILETMMYYRKKKYG
                     A"
BASE COUNT         1675 a         1000 c          982 g         1965 t
ORIGIN      
        1 tatcaagcaa caaacggctc attcccaata tggcattcat gggtgtgcga atttcgtggc
       61 tcatatttgc cagaaactgt tgtttaaact gctcactctt ttcggctcgc tcttttgctt
      121 tttgcactat tacattttgc tcttcgagtt ttatggttgc cttgcgtttt atatcgtatc
      181 gattgtacaa caccaatgca agtaaaagta acatgcttgc cccggccaaa taaatattct
      241 ttaactgctt ttgtttctgt gcttcggcag tgtccaaagc tttttcggca gatagcaatt
      301 ttatcttttc ctcttttatc gtgctttcgt atttgtttac catttccttt tgcgtgatca
      361 tttcatcagc atcataaata ctgtctttca aattatcgca agcttcatag tagtaaaaag
      421 cttgttcaaa acgtcctgtt ttcgaaagtg ttttcgccag gtatttatac acatcagcaa
      481 gtacgctggc atcgtcaatt tctttcgaaa tagtttctga ttccttgagc gtatgcaaag
      541 catttgcata atcgcctaca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntttgg
      601 tcgttggata gttgctctgc aataggaata acatttatgt aaagcgcaat cgctttattg
      661 tacatgctac atttatcgta aatagtgccc aacaaaaatg cacaaaatgg aaggcaagtt
      721 ttggatttgt tgagtgatga taactcaaag aatttcgacc ttattttgat ggatgtacaa
      781 atgccattga tggatggatt tgaaaccaca aattttatac gtaaaaatat gaaagagcct
      841 gtttccaata ttcccatcat tggttttacc gcatctgtaa ttcgcaccga cctcgataag
      901 tgtatagatg ccggcatgaa ttgttatgtt gccaaacctt ttcaagagga agaattaatt
      961 acagctattt taagtttgnn nnnnnnnnna tgatgatgcg gggaaaaatt cggaaattgg
     1021 aaatagtaat attgaatcat cgcaagcgga ttataaaaat ttatttttac agttggtgcc
     1081 accgcgcttg caagttattc agcaggcatt atcaacaaac gatttgcaaa caataaaaaa
     1141 tatggtgcat cttatgcaac cccaattatt gcatgccggc cttatggagc atgcagattt
     1201 atttgcatcg ttcgagcatt ataacgaaaa tactaaacgg gaagagtggc tggaaagtac
     1261 aaaattgttt tgcgaaattg ttgaaactaa attaaagcaa atggaataaa attaatgtgc
     1321 gtatggaaaa aataaaaaca ataattattg acgatgatat ttttattcat gcacaactgc
     1381 gcgagcaact catgttgctg tttcccaaag ttcaggttgt tgctgtttgt gaaaatgctt
     1441 tgcatggcat cgaagcaata aaatcccatc ggcccgattt aatttttctg gatattcaga
     1501 tgcccggcct tagcgggttt gagatgctcg accatgtcga cagaaacaac cttaaagtaa
     1561 tatttatcac ttcgtataat cagtatgcca tagaagccat tcgttacagc gccttcgatt
     1621 atttattaaa accgattaaa gaagaggaat taaaaaatgc catttacaga tttgagcagg
     1681 cgaatgatga aatatccttt aaaggaaaaa ttgagaacct gttacataat ataaacagcg
     1741 aaaaggaagc ctttcaactt ataattccat taaggcaggg agaaaaaaaa ttgcagatta
     1801 aaaatattgt ccggtgcgaa gccgattcaa attatacaca ctttttcctt agcgataaaa
     1861 caaaattcac cgcatcgaaa actttgaagg agtgcgaaga gatgctgtcg cagaaagaat
     1921 ttttgcgtgt acataaatct cacctcatca acaaaagttt tattcaactt cttaaaaacg
     1981 ataatcatgt ggtgcttacc gatggttcgt tgattgaagt ttcgcgcaga aaattgcagg
     2041 aggtaaagaa tagtattcta gagtaaggga gagttttttt tattgccact ttaagtatta
     2101 agaatttcta ttacctgaat acgaaacaca tactcatcag taccatcaat aaaatttgta
     2161 aaggaaagct gacgtatata ccaatagtag caacactaca aaagctagaa tgcattaaca
     2221 ctacacctaa ataatttttc taacgcttcc tcaatgttgc tcaactatta atgcaattta
     2281 atttggtgcc gctgcctgcc ctgccaaagc agagtaacaa ggcatatgct atagtcaagt
     2341 atatgaacct ctccatctgg tttttgtcta atcggctata attaaagttg tgtcagtttt
     2401 ttagttgtgt tctgtttctt gtaagttgaa atattcacct tttgttttgt ctaaggtatt
     2461 agatattttc ttgaaatttt cttctgttgg tttcaagtct ataaactttt ctacgatggt
     2521 ttggtgtttg taaataataa aagtgttttc aacgtttgga tttattttat tgagatttac
     2581 ttcgctttct gtgtccgaca ttgaaggcac aaatgtcaaa gcgatatttt tcaagttgag
     2641 ttcattgcca attttttcta attctttttg tcgtgtgtcc ttcacataat tattttcatt
     2701 tccataaacg aaatacgctt tcaaatattt acttcttgtt acactttcct gctctaaaaa
     2761 agtcagccat tgtttaatgt cgttccaatt gggattgttg cctacaaagt atactattcc
     2821 gtgaaatcgt ccgtacttac aaaccgggca agcccgagat cctttatcag gaccaaatgc
     2881 gtgataagga ataaacgaag gattgtcttc accaatttca agtcctgatt gtttctccgg
     2941 tttatttgtt tcagggtaat tgggaatgtt aagtcccagg ataatattat gttctgctat
     3001 ttgtaagtcg cctgaaggca gaactctcaa aattccactt ccaccacgat tttcaagggc
     3061 ctttcttttt tctccagtaa gatatttatc atcatcaaaa acaaattcgt caatgtagta
     3121 ttcattgtca atgtttggtt ctttgataga agtatgaata tgtgccggta tattttcatg
     3181 aggataaggt gcaggtttga tggtgtttat tgagtatttt ccattttcat ctgtctttac
     3241 ccaacctcgt atgtgtccat ggcgttttgc tttttcgtgc aatccttgtt ttggcgaata
     3301 gtagccgtta ttgtctgttt gccagtaata tatcacaaca tttggtgccg gtgtttttcc
     3361 accaagttta aatactgttc ctgtcactaa taatttttga cctttttctg cccaacctgc
     3421 acttgtatct accgacttaa tatttgccgg cattccaaca tacataattt cgcaaccatc
     3481 acaaccacca cccacaagtt tttttgtgtc ggtattttgc ttcgttgttg cattgccttt
     3541 tgtccgttcg ttacaactta tcaaaaagtt aaacagtgta acaataagta gaaaatttat
     3601 tagccttttc ataatttgta agttatctgt tttggttaga gcataattca tagtgtaagc
     3661 aaagtgaccc atagctacac tttaacatca tgtggtaaaa ataaaaatta caacaacact
     3721 gttgagtaac ttatcaaaaa taaatacaca tcacaaaact gtggaggaac ttgtaaagcc
     3781 gtatatggtc accgttatga tatcggtttg gtaggcaata tttttaaaag cggatttttt
     3841 cacaacctga tggtttgcgg cttggagatg tttcggatta ttaccattta tgttcattag
     3901 aagcttagat gttagcttaa acacaaatgt gtcatcgaag cacgacatca gctttttttg
     3961 cagtgccgca gttatgaggt caacttttat ttctttttga tttttgaaat tgttgcacct
     4021 attgatttag ttggtttgtc catatctttt attgcaaagt caatcgcttc tattatcaac
     4081 gcttttacct ttggattcct gtaatcgttt gactttttta catcaatgtg tcttattaaa
     4141 tttcctgttc ctgtcaacag tttattcggg tctttcaata gcgctccttt attaaagccc
     4201 aagtttagat gatttgtata gataggtagc atacaaaatg cgtccgatag tttttctgat
     4261 attgaaaaaa ctgctgtcaa agcatgagta tgataaatta gctcattgct gtcgggatac
     4321 aggtctaaaa tgtattgacg caaatcgcag aaaaggtcaa tgagttcatg gtctttatat
     4381 ttcaacaaaa attgaaagtc gggatgtatt ggtcttgatt ttttcatctc gctgtattgt
     4441 tgtattgcat tccacaaatt aaatttcctt ctgtgtctaa aaacttggca atccaaccaa
     4501 cataaggtat agccgttttg ggcattaaga tttgtccgcc attaattttt actttttcaa
     4561 taatagcgtc aatactttca acgccaattg tacattccaa acctataatc ttgtcagcaa
     4621 ctggggaata ttttcttgat tgcaatgctc caatcaattc tccgttttcg gacttgtcgg
     4681 ctttgatttg tataaaatcg tcttgtccgt aagaattgaa accccaatca aaaactttgt
     4741 cataaaaatt ttttgcccgt tcaatgtcgt ctatgtgaat ggcaaaatgt gtcaacttat
     4801 ttttcaaatt actttgcttt aattgttatc gtgtcgtttt ttaatttgca gctaacgcca
     4861 gactgtgaaa aaaccaatgt gcccaaagct atacatttaa atcatggtgt aaaaataaaa
     4921 atcatacaac acagttgaca cacctttaca aaaaatattc atcgcttacc tgatgtaaca
     4981 agttgtaaaa ccgtataggg ttaccgttat aaaagcttta gggtcgacaa tatttttaaa
     5041 aaagggtttt ttcacatcct tacggtttgt agcttttata agttggtgat tttcgccact
     5101 aatgatgatg cagtgagcca atgtttgatt aactagaaat gtgtctgagc gctaccgcac
     5161 cgccactttt gccaaagccg ggctatgcac cgtatttctt ctttctataa tacatcattg
     5221 tctccaaaat aattgcactt aacaatgcaa gtagtccaag accaagcgtt atatctttaa
     5281 ttaattttgt cttgctaaaa aacgattcga ttaaaactga acctgcaaca aaataccatg
     5341 aaatattttt tagccgattc agttttctat ttccaattga atttgtccac ttaggataaa
     5401 tgtcattgtc tggataaagt tttaccaata attcaaagtc aggttgagcc aattcgtaat
     5461 ggccaagata gtagttacat agtccacgat agaatattaa agtctcataa aatttcacgt
     5521 ttggtaaagt gtccgctgtg aattttttat tgcttttaat aaggtcaatt gctttgttta
     5581 ttgaaggaat ggctttttta taactttgtg tttctacaag agcaattcca tagtcagcga
     5641 ttagttggga gtaaatgtcg tatgtgtc
//
LOCUS       DAORQA010000103         5652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3641_length_5652_cov_11.011613, whole genome shotgun sequence.
ACCESSION   DAORQA010000103 DAORQA010000000
VERSION     DAORQA010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3641_length_5652_cov_11.011613"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PK736_06250"
     CDS             complement(<1..338)
                     /locus_tag="PK736_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33027.1"
                     /translation="MRITIGMCLAVFFLQINTFAQGLNNLWMHGYSNWGGIPYGGTNI
                     NLYTGDTNIYYHYRNLSLNVTHANIEDTNGNLLFYTNGIHIYNWLDTAMENGKYINPG
                     FNVTQWLNYG"
     gene            611..1597
                     /locus_tag="PK736_06255"
     CDS             611..1597
                     /locus_tag="PK736_06255"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33028.1"
                     /translation="MIQKSIPIINDTLMCGEIVSTKHANGRDYWILVHSLLGNKFYKM
                     LLTPQGISVDTQCIGSNRNLYGGYAKFSLDGSKLAVNSPSTKIDLYDFDRCTGMLSNH
                     ININNPDSSWRGVGFSNDGTKLYYCSGLRVYQYDLNATNIAASVDTVAEYDGFTSLYG
                     FETTFLYPYNGYDGRIYWNCSNGVLELHRIEYPDKLGDSCMFVQHNILCPTFNAFTQP
                     NHPNYHLGPLVGSPCDTLNLTPGPSPKERGVVRVIPNPIKGNILNIEYPVIKNKNNSL
                     KIFDAQGNNVYIRTLPMWSGMHDFPVPNLAPGLYMIQIIDGENRSYGKFCIF"
     gene            1984..3006
                     /locus_tag="PK736_06260"
     CDS             1984..3006
                     /locus_tag="PK736_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33029.1"
                     /translation="MRFLFLSVLFVLAVNCCKGQGINNLWQHGYSTYATLPWNGTQMN
                     LFNGDTSIVYHERKMSFNFTLANISDSSGNMLFYSNGISVANKMDSIMVNGDSLNPSA
                     NTTAWIDDGMPIPQGNIIIPFPNKPNQYLLFHETFVWHKTLPWSVAPELFYSKIDMSL
                     DSGRGALIQKNMFVINDSIIFGEMVACKHANGRDWWLMNHDFNNDVFYKTLITLDTMI
                     TYSQHIGPVRTFFSGFCNFSLDGNKFACVNPDQDLDIYDFDRCSGDLSNRIHIPIADS
                     SVWRTLCFSPDNSKLYVISLLYAYQYDLTAANIASTLDTIAYVEWHARFSGAKFSARF
                     VHDSNY"
     gene            3433..4923
                     /locus_tag="PK736_06265"
     CDS             3433..4923
                     /locus_tag="PK736_06265"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33030.1"
                     /translation="MRFLFLSVLFVLAVNCCKGQGINNLWQHGYSTYATLPWNGTQMN
                     LFNGDTSIVYHERKMSFNFTLANISDSSGNMLFYSNGISVANKMDSIMVNGDSLNPSA
                     NTTAWIDDGMPIPQGNIIIPFPNKPNQYLLFHETFVWHKTLPWSVAPELFYSKIDMSL
                     DSGRGALIQKNMFVINDSIIFGEMVACKHANGRDWWLMNHDFNNDVFYKTLITLDTMI
                     TYSQHIGPVRTFFSGFCNFSLDGNKFACVNPDQDLDIYDFDRCSGDLSNRIHIPIADS
                     SVWRTLCFSPDNSKLYVISLLYAYQYDLTAANIASTLDTIAVWDGFYSPQPPLAAVFN
                     IPYNGYDGRVYVNCANSTFHLHRIEFPDKLGDSCMFTQHNILCPTYNGFTSPNHPNYH
                     LGPLVGSPCDTLNLTPGPSPKERGVVRVIPNPIKGNILNIEYPVIKNKNNSLKIFDAQ
                     GNNVYIRTLPMWSGMHDFPVPNLSPGLYMIQIIDGENRSYGKFCIF"
     gene            5306..>5652
                     /locus_tag="PK736_06270"
     CDS             5306..>5652
                     /locus_tag="PK736_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33031.1"
                     /translation="MKKIIFILMIFCAAIVQAQEKLVQRVQSPLAFNPTLCQLRINPS
                     DTLWRAFKAPASPFKGLPMFVADYNAKMYTVQEYNDFMLLFDKLMNADKNYPTQESTR
                     DDYDMFMRLYVYRG"
BASE COUNT         1713 a          994 c         1074 g         1871 t
ORIGIN      
        1 aagccataat tcagccactg tgtaacatta aaacctggat ttatatattt gccattttcc
       61 attgcagtat caagccaatt gtaaatgtgt ataccgttag tgtaaaatag caagttacca
      121 ttagtatctt caatatttgc atgcgttacg ttcaatgata aatttctata atggtaataa
      181 atatttgtat cccctgtgta aagattaatg ttcgtcccgc catatggtat accaccccaa
      241 ttactatacc cgtgcatcca aagattattg agaccttgtg caaatgtgtt tatttgcaaa
      301 aaaaatactg caaggcacat tccaattgta attcgcatga ttctacaatt ttgtaaactt
      361 atgcactaaa acaaaatcgt catcataggt tatatgcaaa tatataaatc caggttttaa
      421 tgttacacag tggctgaatt atggcttatg gatacaagaa ggggctctca tattgccaaa
      481 accaaatgat agtttgcaat attatgtttt ccatgaggca tatttaaatt atattgttcc
      541 tggcgcactt tatagctccc ccttatatta ttcattagtc gctatgggct ataataatgg
      601 gctaggaaaa gtgatacaga aatcaattcc aataattaat gatactttga tgtgtggcga
      661 aattgtgtca acaaaacatg caaatggcag ggactattgg atcttagttc atagtttatt
      721 aggcaacaag ttttataaaa tgttgctaac accacaaggt attagtgtag acacacaatg
      781 tattggttct aacagaaact tatacggtgg ttacgcaaag ttcagtttag acggttcaaa
      841 attagctgtc aatagcccaa gtacaaaaat agatttatat gatttcgaca ggtgtacagg
      901 tatgttgagc aatcacataa atattaataa tccagatagt agttggcgag gtgttggctt
      961 ttcaaatgat ggcacaaaat tatattattg ctcaggcctg cgtgtttatc aatatgacct
     1021 taatgctact aacatagcag catcggtcga caccgttgct gaatatgacg gctttacttc
     1081 tttgtatggt ttcgagacaa catttttata cccctataat ggatatgatg gtcgcattta
     1141 ctggaattgt tctaatggtg tgttagaact tcatcggata gagtatcctg ataagttagg
     1201 tgattcgtgt atgtttgtac agcataatat attatgtcct acatttaatg catttacaca
     1261 acccaaccac cccaactacc acttaggtcc gcttgtgggt agcccttgcg atacgctgaa
     1321 cctcaccccc ggcccctctc caaaggagag gggagtcgtt cgcgttattc ccaatcctat
     1381 aaagggtaat attttaaaca ttgagtatcc tgttataaaa aacaaaaaca acagcttaaa
     1441 aatatttgac gcccagggta acaatgtgta tatacgtacc ttgcctatgt ggagtggcat
     1501 gcacgatttt ccggtgccaa atttagcgcc cggtttgtac atgattcaaa ttattgacgg
     1561 ggagaatagg agctatggga agttttgcat tttttagttc attgtacttt tgttcttcgt
     1621 ttcgtttgct gagagttttg tgctttgtcc atcatggtga gcttgtcaaa gcgagagtga
     1681 tggtgagcaa attgcgaact aaagcacgca cggttgaatt gttttgaatt gtcaatgtcc
     1741 acaatatttc ctcccgtcat tgtaagcgta gcgaagtaat ctcatcgtag gaatggtgcc
     1801 ggaagattgt cattgattat tgtgataatc ataacaaggc taaagtaaaa tcaacacaac
     1861 aaccaatcgt cacgtgagat tgcttcagtc gtacctcctt cgcaatgact acaagaaaaa
     1921 ttcacacaat aactaataac cattacattt gtatatggca tctaaattaa ttagctggaa
     1981 aaaatgagat ttttattctt gtccgtttta tttgttttgg cagtgaattg ttgtaaagga
     2041 caaggtataa acaatttatg gcaacatgga tactccacgt atgcaacatt gccgtggaat
     2101 ggcactcaaa tgaatttatt taatggtgac acatctatag tttatcatga aagaaaaatg
     2161 tcgttcaatt ttactttagc aaatatttcc gattcgagcg gcaatatgtt attttattcg
     2221 aatgggatat ctgtggccaa taaaatggat agtataatgg taaatggtga tagcttaaac
     2281 ccaagtgcca atacaactgc atggattgat gatggtatgc ccattccaca aggtaatatt
     2341 attatacctt ttcctaataa gcccaatcaa tatttattgt ttcatgaaac ctttgtttgg
     2401 cataaaacct taccgtggag tgtagcaccc gaattatttt attcaaaaat agacatgtct
     2461 ctcgattcgg gacgcggtgc cctaatccaa aaaaacatgt ttgtcatcaa tgactcaatt
     2521 atttttgggg aaatggtagc gtgtaaacat gctaacgggc gcgattggtg gctaatgaat
     2581 cacgatttta ataatgatgt gttttataaa acattgatta cactggatac catgataact
     2641 tatagtcaac acataggtcc agttcgtact tttttttcag gattctgtaa ttttagttta
     2701 gatggtaata aatttgcatg tgtaaatccc gaccaagatt tagatatata tgattttgat
     2761 agatgcagtg gcgatttaag taatcgtatt cacattccaa ttgccgatag ttccgtttgg
     2821 cgtacccttt gcttttcgcc cgataattct aagttatatg taattagttt gctttacgca
     2881 taccaatatg atttaacagc agcaaacatt gcatctacgt tggataccat tgcctatgtg
     2941 gagtggcatg cacgattttc cggtgccaaa tttagcgccc ggtttgtaca tgattcaaat
     3001 tattgacggg gagaatagga gctatgggaa gttttgcatt ttttagttca ttgtactttt
     3061 gttcttcgtt tcgtttgctg agagttttgt gctttgtcca tcatggtgag cttgtcaaag
     3121 cgagagtgat ggtgagcaaa ttgcgaacta aagcacgcac ggttgaattg ttttgaattg
     3181 tcaatgtcca caatatttcc tcccgtcatt gtaagcgtag cgaagtaatc tcatcgtagg
     3241 aatggtgccg gaagattgtc attgattatt gtgataatca taacaaggct aaagtaaaat
     3301 caacacaaca accaatcgtc acgtgagatt gcttcagtcg tacctccttc gcaatgacta
     3361 caagaaaaat tcacacaata actaataacc attacatttg tatatggcat ctaaattaat
     3421 tagctggaaa aaatgagatt tttattcttg tccgttttat ttgttttggc agtgaattgt
     3481 tgtaaaggac aaggtataaa caatttatgg caacatggat actccacgta tgcaacattg
     3541 ccgtggaatg gcactcaaat gaatttattt aatggtgaca catctatagt ttatcatgaa
     3601 agaaaaatgt cgttcaattt tactttagca aatatttccg attcgagcgg caatatgtta
     3661 ttttattcga atgggatatc tgtggccaat aaaatggata gtataatggt aaatggtgat
     3721 agcttaaacc caagtgccaa tacaactgca tggattgatg atggtatgcc cattccacaa
     3781 ggtaatatta ttataccttt tcctaataag cccaatcaat atttattgtt tcatgaaacc
     3841 tttgtttggc ataaaacctt accgtggagt gtagcacccg aattatttta ttcaaaaata
     3901 gacatgtctc tcgattcggg acgcggtgcc ctaatccaaa aaaacatgtt tgtcatcaat
     3961 gactcaatta tttttgggga aatggtagcg tgtaaacatg ctaacgggcg cgattggtgg
     4021 ctaatgaatc acgattttaa taatgatgtg ttttataaaa cattgattac actggatacc
     4081 atgataactt atagtcaaca cataggtcca gttcgtactt ttttttcagg attctgtaat
     4141 tttagtttag atggtaataa atttgcatgt gtaaatcccg accaagattt agatatatat
     4201 gattttgata gatgcagtgg cgatttaagt aatcgtattc acattccaat tgccgatagt
     4261 tccgtttggc gtaccctttg cttttcgccc gataattcta agttatatgt aattagtttg
     4321 ctttacgcat accaatatga tttaacagca gcaaacattg catctacgtt ggataccatt
     4381 gctgtatggg atggctttta ttcgccacag ccgcctttgg cagctgtttt taatattccc
     4441 tacaatggat atgatggtcg tgtttatgtt aattgtgcaa atagcacttt tcatttacac
     4501 cgtatagagt ttcctgataa attaggcgat tcgtgcatgt tcacgcagca caatatttta
     4561 tgccctactt ataatgggtt cacttctccc aaccacccca actaccattt aggtccgctt
     4621 gtgggtagcc cttgcgatac actgaacctc acccccggcc cctctcccaa ggagagggga
     4681 gtcgttcgcg ttattcccaa tcctataaag ggtaatattt taaacattga gtatcctgtt
     4741 ataaaaaaca aaaacaacag cttaaaaata tttgacgccc agggtaacaa tgtgtatata
     4801 cgtaccttgc ctatgtggag tggcatgcac gattttccgg tgccaaattt atcgccaggg
     4861 ttgtacatga ttcaaattat tgatggggag aataggagct atgggaagtt ttgcattttt
     4921 tagttcattg ttcttttgtt cttcgtttcg tttgctgaga gttttgtttt ttcttctctg
     4981 ttcggtagtt tgaattaaat tcagagtctc tcatgccatt aaaaaacaat aaataaatga
     5041 ggactctgaa attcgttttt cgtttcgttt gctgcgagcc atgttctttc ttcttagatt
     5101 ggggaagttt gaattaattt cagagtctct catgcaattg aaaaacaata aataaatgag
     5161 gactctgaaa ttcgttttat acataaaatc atggttggag taagctgttt tttatttatt
     5221 tcacaatcat caaaataatt tcttcgacaa taaatattat atttgtacaa agtattttga
     5281 atcgatgaac tagaaaataa acggtatgaa aaaaataatt tttattttga tgatattttg
     5341 tgcagcaata gtgcaagcgc aagaaaaact tgtgcaacgt gtacaatcac ctttagcatt
     5401 taaccccaca ctatgccaat tgcgcataaa ccccagcgat acactgtggc gtgcatttaa
     5461 ggcaccggca tcgcccttca aaggattgcc aatgtttgta gccgactaca atgcgaagat
     5521 gtataccgtg caagagtaca acgactttat gctgttgttc gacaagctga tgaatgctga
     5581 taaaaattat cctacacaag aatccacccg cgatgattat gatatgttta tgcgcttgta
     5641 tgtatataga gg
//
LOCUS       DAORQA010000104         5649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3645_length_5649_cov_7.232034, whole genome shotgun sequence.
ACCESSION   DAORQA010000104 DAORQA010000000
VERSION     DAORQA010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3645_length_5649_cov_7.232034"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PK736_06275"
     CDS             <1..816
                     /locus_tag="PK736_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33032.1"
                     /translation="QITAGYLNVGPDFRSIGAQSKDVNYNAQPAYFNRYTNAQQIRPL
                     ALFDIINNENIYNRTITARQMSESQIYNNVLPFGLATFNRIGMYAKVEYKSKKEISIN
                     AEYYNLSEIRGQGSLALKQFSIYKMNANFPINKLINSTKVLSVQIGGNMQQTIRSSEQ
                     EIEKIDLQSMQLSAGLRWEFASNFELLGGYISQENDGNEYKADRNSYDVITYFNPVNY
                     KIKQQIVAGGLRYNFTSKIYLCGLYQQSTFTDKLKTTADFGINQFGLIFNMTF"
     gene            818..1822
                     /locus_tag="PK736_06280"
     CDS             818..1822
                     /locus_tag="PK736_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33033.1"
                     /translation="MKQKAAIIFILLTGLISCRKDETNFDGPGVVDIYSDFKIIENFD
                     ADKDSVNFANNETVLFTAKFSKIVKWQIAIKGKVSKATKIFNGESKAINITNGLWNGS
                     TTNYPIFGIENCTASLTLNAIPDTFNIDVKVTSPKKIDGFIIADFENGLNPQWPKFIQ
                     SGANMDFKVKTDSLAPEGKKYMNMAGTVNWDYLIGLIDFPAQAYNSNVTFPLLPNPGA
                     VYFNCLIYGVPNTNPSIVLFQLKEDENSDGTFNANNEDEYDLQINVDWEGWKLVSVKY
                     EDIVTLVNGSPANPKGNGIRNPDKLGKISMLHLADPNNGFASTKIDMIVITDNKPLEP
                     "
     gene            1822..2466
                     /locus_tag="PK736_06285"
     CDS             1822..2466
                     /locus_tag="PK736_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33034.1"
                     /translation="MKGQSLFSGVAIISCMFCSCKSSFISVQNKSLYDENAPVNTNQI
                     EGFTAIDIYKDTYDNSVWVSPESNCVEMKSEKQNTFSGNGALHIKWDKIKGGCKWIGC
                     GFGWNNWMAKDIKDIDSIAAIQMKVKSVKGSFKNLPVAFALEDYSGVQCYYGFRLELA
                     SGIFNDTAWTSVTIPLSKFDFENKNFDLEKVKQFIIQLEGDGNIYLDDIKFIRL"
     gene            2468..4717
                     /locus_tag="PK736_06290"
     CDS             2468..4717
                     /locus_tag="PK736_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRC33035.1"
                     /translation="MIRSAILFVFLFCATNFFVSAQKTADVNIESLINSMTIEEKVGQ
                     MTQIDLGVIANGEICNLQNPQSINSEKLKIAISQYHVGSILNVGCASGTISLSNWHNI
                     ISTIQNENTKLDRNKIPIIYGIDAIHGANYTMGATLFPQQIAMAASWNPALVEKGFAI
                     TAYETRASGLPWNFSPVLDIGRQPLWSRFFETFGEDVCLAKSMTTAAINGLQGNNINN
                     PYKVAACMKHFLGYSMPLSGKDRTPAWISDRELREYFLPTFEQAIKSGAKTVMINSGE
                     INGTPVHVNYDILTKLLREELGFKGVAVTDWEDIYKLVNLHRVAENKKEAVRMAIMAG
                     IDMSMTPNDFEFNKLLIELVKEGTIPEARINISVRRILELKKELGLFSQTQFTQNNYP
                     LFGSKHAEVSYELAAECITLLKNKNNLLPLKNEKQNILVCGPAAQSLNLLNGAWTHTW
                     QGVDTKYNTANKLTILDAIKINAGNATIQYALGSSLDSVMDLNDCFAKAKNADKIIVC
                     LGELPCTELPGNIDDLSLPLAQQILVNKLKEAGKPIIFICCFNRPRILQAVAKYADAI
                     VYAYLPGDEGGRAIADCLYGKINPSGKLPFTYPANVNAIVHYDHKTTEDQDVDFSANA
                     YRPQFDFGFGMSYTKFDYSNFSVSQNTLNDNDSIVIKVEVKNSGAVNGKEIVQVYYKD
                     LFASITPAVKKLVAFKKINLNASQTTQVEFVIHKNDLSFINKDLKRVTEKGDFEIMIN
                     NEKKLINVK"
     gene            4707..5546
                     /locus_tag="PK736_06295"
     CDS             4707..5546
                     /locus_tag="PK736_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33036.1"
                     /translation="MLNRKKASVIYIALVLLYGGCKQSAPTDVVVADEKGDLTFSGYN
                     WKYKNALTPVGPGPNRFLGTAENVWVDNAGSLHMRISKKNNFWYCSEIISVKEFGYGT
                     YVFTCENDIRNLNEKNVFGFFTWDDYSFQKEGNSEVDIEFSKWNNPNDTLVLTYSVQP
                     VIFQNPVPYSERTYKPPIATSLISKATTHMMRWTPDSVYWESYEGEFYPGSNKIASWS
                     FDKNNLPRSKLENNLVSDPIVIPGPGDSTNLRFNYWLLNGQPPTNGLEYEMVIKSFKY
                     FPL"
BASE COUNT         2032 a          948 c         1046 g         1623 t
ORIGIN      
        1 caaatcaccg caggatattt aaacgttggt cccgatttta gaagcattgg cgctcaaagc
       61 aaagatgtaa actataatgc acagcctgca tatttcaaca ggtataccaa cgcacaacaa
      121 attcgtccgc ttgcattgtt cgatatcata aataatgaaa acatatacaa ccgcactata
      181 actgcaaggc aaatgagcga aagtcaaatt tacaataatg ttcttccttt tggattggct
      241 acctttaaca gaataggaat gtatgcaaag gtggagtaca aatcgaaaaa ggaaatatca
      301 ataaatgccg aatattataa tttaagcgaa ataagaggtc agggttcatt ggcgttgaaa
      361 cagttcagca tttataaaat gaatgcaaat tttccaatta ataaattaat aaacagcacc
      421 aaagttttat cggttcaaat aggtggcaac atgcagcaaa ctataagaag cagtgaacag
      481 gaaattgaaa aaatagattt acaatccatg caattaagtg cagggcttag atgggaattt
      541 gcttccaatt tcgaattact tggaggatac atcagccagg aaaatgatgg caacgaatat
      601 aaagccgacc gaaattcata cgatgtaatt acttatttca atcctgtaaa ttacaaaata
      661 aaacaacaga tagttgccgg tggtttgcgg tataatttca catcaaaaat ttatttgtgt
      721 ggcctgtatc agcaaagcac atttaccgat aaacttaaaa caactgccga ctttgggatt
      781 aatcaatttg gtttaatatt taacatgaca ttttaaaatg aaacaaaaag cagcaataat
      841 ttttattctt ttaacaggat tgatttcttg cagaaaagac gaaacaaact tcgatggccc
      901 gggcgttgtt gatatataca gcgattttaa aatcatagaa aatttcgatg ccgataaaga
      961 cagcgtaaat tttgccaaca acgaaactgt attgttcaca gcaaaatttt ccaaaattgt
     1021 aaagtggcaa attgccataa aaggcaaagt atcaaaggcc accaaaattt ttaatggcga
     1081 aagcaaagct ataaatatta ctaatggatt atggaatggc agcactacca actaccctat
     1141 ttttggtatt gaaaactgca ctgcttcact cacactgaat gcaattcccg atactttcaa
     1201 tattgatgtt aaagttacaa gtccaaaaaa gatagacggt tttattattg ccgatttcga
     1261 aaacgggctc aatccacaat ggcccaaatt tattcaatcg ggtgctaata tggacttcaa
     1321 agtaaaaaca gattcgcttg cacctgaggg caaaaaatat atgaatatgg ccggaactgt
     1381 aaattgggat tacctaatcg ggcttatcga ttttcctgcg caagcgtata actccaatgt
     1441 tacttttccg ttgttaccaa accccggtgc ggtgtatttc aattgtttaa tttatggtgt
     1501 gcccaataca aatccttcta tcgttttatt tcaattgaaa gaagatgaaa atagtgatgg
     1561 cacttttaat gcaaacaacg aagatgagta tgaccttcaa attaacgttg actgggaagg
     1621 ctggaaattg gtatcggtaa aatacgaaga cattgttaca cttgtaaatg gctcgcctgc
     1681 aaatccaaaa ggaaatggca ttcgtaatcc cgataagttg ggaaaaattt caatgttaca
     1741 tcttgccgat ccaaacaatg gctttgcaag tactaaaata gatatgattg taattaccga
     1801 taacaaacca cttgaaccat aatgaaaggc caaagtttat tttcgggtgt ggctattatt
     1861 tcatgcatgt tttgctcgtg caagtcatct tttatttctg ttcagaataa aagtttgtat
     1921 gatgaaaatg cgcctgtaaa tacaaatcaa attgaaggat ttactgctat cgatatttat
     1981 aaagatactt atgacaatag cgtatgggta tcgcccgaaa gcaattgtgt agaaatgaaa
     2041 tctgaaaagc aaaatacttt ttcgggtaat ggtgccttgc atataaaatg ggataaaata
     2101 aaaggtgggt gtaaatggat aggatgcgga tttggatgga acaactggat ggccaaagac
     2161 attaaagata ttgatagcat agctgccata caaatgaaag taaaatctgt aaagggatcg
     2221 ttcaaaaatt tacccgtggc gtttgcttta gaagattact ctggcgtgca atgttattat
     2281 ggtttcagat tggaacttgc ctcaggtata tttaacgaca ctgcatggac ttcggtaaca
     2341 ataccacttt caaaattcga tttcgaaaat aaaaattttg atctcgaaaa agtaaaacaa
     2401 tttattattc aactcgaagg tgacggcaac atttatttag acgatataaa atttatacga
     2461 ttataaaatg ataaggtcgg caatcttatt tgtgtttctt ttttgtgcaa caaacttttt
     2521 tgttagtgca caaaaaacag cagatgtaaa tattgagtcg ctcataaatt ccatgaccat
     2581 tgaagaaaaa gtgggacaaa tgactcaaat agatttaggc gttattgcta atggcgaaat
     2641 ttgtaatttg caaaatcccc aatctataaa ttccgaaaaa ttaaaaattg ctatttctca
     2701 atatcatgta ggatcgattt taaatgttgg ttgtgcaagt ggaactattt cattaagcaa
     2761 ctggcataat attatttcta ctatacaaaa cgaaaataca aaattagaca gaaacaagat
     2821 tcctataatt tatggcattg atgcaataca tggtgcaaac tatacaatgg gtgcaacatt
     2881 gtttccacaa caaatagcaa tggcagcatc atggaatcct gcattggttg aaaagggatt
     2941 tgccatcacc gcctatgaaa cacgtgcaag tgggttgcca tggaattttt cgcctgtact
     3001 tgatattgga cgacaaccat tatggtcgcg attttttgaa acctttggag aagatgtttg
     3061 ccttgcaaaa agcatgacca ctgcagccat aaatggttta caaggcaaca acataaacaa
     3121 cccgtataaa gtagctgcat gcatgaaaca ctttcttgga tacagcatgc cactaagcgg
     3181 aaaagaccgt acgcctgcat ggatttcgga tagagaattg cgcgaatatt ttttacccac
     3241 gtttgagcaa gccataaaaa gtggtgccaa aacggtgatg ataaacagcg gtgaaattaa
     3301 cggcacaccc gtacatgtta attacgatat acttacaaaa cttttgcgcg aagaactcgg
     3361 atttaaaggt gtagctgtta ccgattggga agacatttac aaacttgtca atttacatcg
     3421 cgttgccgaa aataaaaagg aagcggtgcg tatggcaata atggccggca tcgatatgag
     3481 tatgaccccc aatgattttg aatttaataa attacttatc gaacttgtaa aagaaggaac
     3541 catacctgag gcacgaatta atatttcggt gcgaagaatt cttgagttaa aaaaagaatt
     3601 aggacttttt tctcaaacac aatttactca aaacaactat ccattatttg gctcgaaaca
     3661 tgccgaagtg agttatgaat tggcagcgga atgcattaca cttttaaaaa ataaaaacaa
     3721 tctattgcca ttaaaaaatg aaaaacaaaa tattcttgta tgtggcccag ccgcacaaag
     3781 tttaaattta ttgaatggcg catggacgca tacatggcag ggagtagaca ctaaatataa
     3841 cacagctaac aaactaacca tacttgatgc tataaaaata aatgcaggca atgctacaat
     3901 tcaatatgca ttgggtagta gtttagatag tgtaatggat ttaaacgatt gttttgcgaa
     3961 agcaaaaaat gccgataaga ttattgtttg tttaggcgaa ctgccttgca ccgaattgcc
     4021 aggaaatata gatgacctat cgcttccgct agcgcagcaa atacttgtga ataaacttaa
     4081 agaagccggc aagcccatta tttttatttg ttgtttcaac aggcctcgca tattacaagc
     4141 tgtagcaaaa tatgccgatg caattgttta cgcttattta ccgggcgatg aaggcggccg
     4201 ggctattgcc gattgccttt atggaaaaat aaatcccagt ggaaagttac catttactta
     4261 tccggcaaat gtaaatgcta tagtacatta cgatcataaa accaccgaag atcaggatgt
     4321 agatttttcg gctaatgctt accgtccaca attcgatttt ggttttggaa tgagttacac
     4381 aaaattcgat tacagcaatt tcagtgtatc acaaaacacc ttaaacgata acgactctat
     4441 cgtaattaaa gtagaagtaa aaaattccgg agcagtaaat ggtaaagaaa tagtgcaggt
     4501 atattacaaa gatttattcg cctccattac accagctgta aaaaaacttg ttgcttttaa
     4561 aaaaataaat ttaaacgcat cgcaaacaac acaagtagaa tttgttatac ataagaacga
     4621 tttatccttt ataaataaag atttgaaaag ggtaaccgaa aaaggagatt ttgaaatcat
     4681 gatcaataat gaaaaaaaat taatcaatgt taaatagaaa aaaagcttct gttatttaca
     4741 ttgcactagt gttgctttat ggtggatgta aacaatcggc accaacagat gtggttgttg
     4801 ccgatgaaaa aggtgacctc acattttcgg gatataactg gaaatataaa aatgcactta
     4861 ctccggtagg ccccgggcca aaccggtttt taggcactgc cgaaaatgtg tgggttgata
     4921 atgcaggaag tttacacatg cgtatatcca aaaaaaataa tttttggtat tgcagcgaaa
     4981 taataagtgt aaaagaattt ggatatggta cttatgtttt tacctgcgaa aatgatatcc
     5041 gaaatttgaa cgaaaaaaat gtctttggct tttttacctg ggacgattat tcatttcaaa
     5101 aagaaggaaa cagcgaagta gatatagagt tttcgaaatg gaataatccc aacgatactt
     5161 tagtattaac ctatagcgtg cagcctgtaa tatttcaaaa cccggtgcca tactccgaaa
     5221 gaacttataa accgccaata gctacaagtc tcataagtaa agcaactaca catatgatgc
     5281 gatggactcc cgatagtgta tactgggaaa gttatgaagg agaattttat ccggggtcaa
     5341 ataaaattgc ttcatggtca ttcgataaaa ataatttacc acgcagcaaa ctcgaaaaca
     5401 acttagtatc ggatcctatt gtaattcccg gtccgggcga ttctacaaat ttaagattca
     5461 actattggtt attaaatggg caaccaccca ccaatggttt agaatatgaa atggttatta
     5521 aatcgttcaa atattttcct ttataaaaaa attaaaacaa aataatatga tgaaaaaaac
     5581 actactattt attcaaagca tgttagttgc aagttgctta tttgcacaaa cacaaaatgt
     5641 aacattcca
//
LOCUS       DAORQA010000105         5644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3650_length_5644_cov_8.311505, whole genome shotgun sequence.
ACCESSION   DAORQA010000105 DAORQA010000000
VERSION     DAORQA010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3650_length_5644_cov_8.311505"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2840
                     /locus_tag="PK736_06300"
     CDS             <1..2840
                     /locus_tag="PK736_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33037.1"
                     /translation="IKQTMEVVLLDNQYTSIQNNVVHIPKWEKLEIGVKLPQEYNDAI
                     TNFFNHYYTVGVNQDKDLNPYADDSLILQAVFNSPTGGAPKIRNGFFMRMAKWDNTNG
                     DFTGEIANSNDDVLVVDNANALSQYCIRFRFAPTEESTTPWTFSLSIKAPNVKDANNQ
                     PLPDLFVEGLQFICDPELTDNKGFLSVNPINKRYFKFDNGETFFGIGNNYADNIKWFT
                     ETNSADVPPKPIITYKYMQKRDQDLRKNALTELHTAGANWMRIMLFKENYSIEHEYNG
                     VYDDYQNTPLCCEEGIGPYVCSLNVNLNSINEPYPIEDFSNRNGNRQAHAWAMDDLLD
                     QARQQNMYLQICIDPYHAGNAYQNFQWGDQCYFKNYVEKDYLDNYKNGINIINAKKYF
                     ISNPGQNTGSLYYWKRMYKYYMARYGYSVNIGEFEMFNEIDGTLTWSNPPSWQPGLDA
                     SMCASLKCAHPVYADPLLKPTINNFINEIVPHAKNVLGQSNRIFSLSYLDNYFLGYYP
                     GLSPYPDAKNYFNLLDNSNIDVADMHAYSTSDHEQDMNAVRHDYTKHFTGPNPVWDMG
                     LDKPYQMSEGSSFMKGDLDISFDQNGSIKTQLPNFYNNYETTFHNTLWSSVFMGTCVN
                     TMSWFWPVVHWVPKGVPEPLQESKGGNLAINPNGNNTLNGVNVIQPEQIPNTKVLIDN
                     HKLINNYTTLNYFINQTQMSFDDNFVSHKKYYASTLQPNLDSLEVYYLTTTDLLHAYG
                     WVHNVKKYWQNECYYKTPDGSSNFRSNYYGCKSLTLANIPPTEFLVDGLLPFDTYDID
                     FYSTWSDNLVVPNSSMVNANSVGQVSVQMTGATIGWQHDGDYAFRIKKHGLPARISAS
                     DNKNDKSKNGFDFYVQPNPNNGLFQIIMPKLETENLIVSIYNQLGVLVYNDKTHYAQI
                     INFDIELHPSGVYHAIIIAGDKVSNQRFIKQ"
     gene            2844..4445
                     /locus_tag="PK736_06305"
     CDS             2844..4445
                     /locus_tag="PK736_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33038.1"
                     /translation="MRKILFTIVWVASFSITLLKSQPFRKLYDVQKLGGNSLVEFQNG
                     TIFMPIGQISKISPNGNFSIGINTASNGGYLYQAIKINNQSMIAVGSFNDSCVDPVEN
                     FPFISYLDTNFNFTNGRHYRSYGLCRLGSNGIERLESNNYLTYGRGYKWGSIPINPFA
                     LLVDNTLDSVIFCKYFSRKGNCVFGKQLPDGNIMLGANFDSIGACVIKLDSIGNILWC
                     KSIVRPTGIFLSAVVNNDGTLMITGIDDYLDWNFAFNGPYPANYHPQLFIMKMDTGGT
                     VLWCKGYSSTFKGQWIAGQPAPIIKTFDDNFVFAALKITDKTYLPNTGAYVKLLKVDA
                     NGDTIYTRGYGAPSGRDTYLGNLIQSKDTGYLITGNFEGKPFSFGGSYLFKTDTGGYT
                     PCGGMYDEPLNMTPLFFTDSLMSLSSIDGADVLPAFATPLFSTSVITADDCIVLANPT
                     TSPQSLYSWPYPNPTKGEVNIQKSNNVPLSYITVYNHQGILVLQKKLEQDEEGKIDLS
                     KFGPGLYLIKINEQGKIRTEQVVVE"
     gene            4776..>5644
                     /locus_tag="PK736_06310"
     CDS             4776..>5644
                     /locus_tag="PK736_06310"
                     /inference="COORDINATES: protein motif:HMM:NF023504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33039.1"
                     /translation="MSTTKLPPRQKMINMMYLVLTAMLALNISKEVINAFVTVNTSLE
                     KSFDNQKVKKQRMFELFKQQMQHDSLRAQLAYSKALVVSQQSDKVCANIETLKNRLIN
                     ETEGVKQAASFISLQDVKRKDAYEKVTNIMCGDDEQGRGAEATKLKQNILNYKQSVLT
                     TLDEKDHIIFKPLLDKNFDTSDPAKDDVAYKEDHKTTWEMKYFYQTPVVASVAMLSKI
                     QTDVKNAEAEVLEHLLNGITADKNTFDKLVAQVVMPTDYIFTGQPVKADIFLTAYNST
                     LSSDVNVNGQQIT"
BASE COUNT         1918 a          966 c         1035 g         1725 t
ORIGIN      
        1 gtataaaaca aaccatggaa gttgttttgt tagacaatca atatacatca atacaaaaca
       61 atgtagtgca tataccaaaa tgggaaaagc tcgaaattgg agttaagttg ccacaggaat
      121 ataatgatgc aatcacaaat ttttttaatc attattacac tgttggggta aaccaagata
      181 aagatttaaa tccttacgct gatgattcgc taatactgca ggctgttttt aatagcccaa
      241 ccggtggtgc accaaaaatc cgtaatggtt tttttatgcg catggcaaaa tgggataata
      301 ccaatggcga ttttacagga gaaattgcaa attctaatga cgatgtattg gtggttgaca
      361 atgctaatgc attgagccaa tattgtatcc gttttcgctt tgcacctaca gaagaatcaa
      421 ctactccctg gacattcagc cttagcatta aggcccctaa cgtaaaagat gcaaacaatc
      481 aaccattgcc cgatttgttt gttgaaggat tgcagtttat ttgtgaccct gaattaacag
      541 acaataaagg tttcttgagt gtgaatccaa tcaataaacg gtattttaaa tttgataatg
      601 gtgaaacctt ttttggtatt ggtaataatt atgccgacaa tattaaatgg tttaccgaaa
      661 caaattcagc tgatgtgcct ccaaagccaa ttattactta taaatacatg caaaagcgcg
      721 atcaggattt gcgtaaaaat gctttaaccg aattgcatac agccggtgcc aattggatgc
      781 gaataatgtt attcaaagaa aattatagta tagaacatga atataatggt gtgtatgacg
      841 attaccaaaa cacaccttta tgttgtgaag aaggaatagg tccttatgta tgcagcctaa
      901 atgtaaattt aaattcaata aatgaacctt atcccattga ggatttttcg aatcgcaatg
      961 gaaacaggca ggcacatgcc tgggctatgg atgatttgct cgaccaagca cgccaacaaa
     1021 atatgtatct tcaaatttgt atagacccat atcatgcagg taatgcatat caaaattttc
     1081 aatggggcga ccaatgctat ttcaaaaatt atgtggagaa agattacctt gataattata
     1141 aaaatgggat aaacatcata aatgcaaaaa aatatttcat cagcaatccc gggcaaaata
     1201 ctggaagcct ctattattgg aagcggatgt acaaatatta catggctcgt tacggttatt
     1261 cggtaaacat tggcgaattt gagatgttta atgaaattga tggcaccctc acttggagca
     1321 accctccttc ttggcaaccg ggtttagatg cttctatgtg tgcttcattg aaatgtgcac
     1381 atccagttta tgctgacccg ttattaaaac caactatcaa taattttatt aatgaaattg
     1441 taccgcatgc taaaaatgtt ttagggcaat caaatagaat attttcgctc tcttatttag
     1501 acaactattt tttaggatat taccctggat tgagtcctta ccctgatgct aaaaattatt
     1561 tcaatttgct tgacaatagc aatatagatg tggcagatat gcacgcatat agcacatccg
     1621 accacgagca agatatgaat gcggtaaggc atgattatac caaacatttt acaggaccaa
     1681 atcctgtttg ggatatgggc ttagataaac catatcaaat gagcgaaggt agtagtttta
     1741 tgaaaggcga tttagatatt tcattcgacc aaaatgggag cataaaaact caattgccaa
     1801 acttttacaa caattacgaa accacttttc acaatacatt atggtcatct gtttttatgg
     1861 gtacttgtgt aaatacaatg agttggtttt ggcctgttgt acattgggtg cctaaaggag
     1921 taccagagcc tttgcaagaa agtaaaggag gtaatcttgc aatcaatcca aatggaaata
     1981 atactttaaa tggcgtgaac gtaatacagc ctgagcaaat tcctaacact aaagttctaa
     2041 ttgataatca taaattgatt aacaattata caacacttaa ctattttatt aaccaaaccc
     2101 aaatgtcgtt tgatgataat tttgttagcc ataaaaaata ctacgcttca actttacaac
     2161 caaacttaga tagtttggaa gtatattatt taaccactac agatttgttg catgcttatg
     2221 gatgggtgca taatgttaaa aaatattggc aaaatgaatg ttattataaa actccagatg
     2281 gaagttcaaa tttcagaagt aattattatg gttgtaagag tttaactttg gccaacattc
     2341 caccaactga atttttggtt gatggtttgc tcccttttga cacttatgat attgattttt
     2401 attctacttg gagtgataat ttggtagtcc caaattcctc aatggttaat gcaaattccg
     2461 ttggccaagt tagtgtgcaa atgacagggg caactatagg ctggcaacac gatggtgatt
     2521 atgctttccg tataaaaaag cacggtttgc cagcacgtat ttccgcctca gataacaaaa
     2581 atgataaaag caaaaatgga tttgattttt acgttcaacc aaaccctaat aatggtttgt
     2641 tccaaattat tatgcctaaa ttagaaacag aaaatttaat tgtaagcata tacaatcaat
     2701 taggggtgct ggtttataat gataaaacac attatgctca aataataaat ttcgatattg
     2761 aattgcaccc ttctggtgta taccatgcta taattatcgc gggagataaa gtttcaaatc
     2821 aacgttttat taaacaataa gttatgagaa aaattttatt tacaattgtg tgggttgcat
     2881 cattttcaat aaccttacta aaatcacagc ctttccgcaa actttatgat gtacaaaaat
     2941 taggtggcaa ttctttggtc gaatttcaaa atggaaccat tttcatgcca ataggtcaaa
     3001 tttcaaaaat atcaccgaat ggtaatttct caattggaat taatactgct tcaaatggag
     3061 gttatctata tcaggcaatc aaaataaata atcagagcat gattgccgtt ggttctttca
     3121 atgattcttg tgttgaccct gttgaaaatt ttccatttat ttcgtatttg gatacaaatt
     3181 ttaatttcac taatgggaga cactatagaa gctatggtct ttgtagattg ggaagcaatg
     3241 gtattgaaag attggagtca aacaattatt taacctatgg taggggatat aaatggggta
     3301 gtattccaat aaacccattt gcacttttag ttgacaatac attggacagc gtaatatttt
     3361 gcaaatattt ttcgcggaag ggcaattgtg tttttggaaa gcaattaccc gatggtaata
     3421 ttatgcttgg tgccaatttc gatagtatag gcgcatgtgt aataaaactt gattctattg
     3481 gaaacatttt gtggtgcaaa tcaatagtcc gaccaacggg tatttttttg agtgcagtgg
     3541 taaacaatga tggtacactt atgattactg gtatagatga ttatttagat tggaattttg
     3601 ctttcaatgg cccatacccg gccaactatc acccacagtt gtttattatg aaaatggaca
     3661 ctggtggcac ggtgctttgg tgcaaaggat acagtagcac cttcaaagga caatggattg
     3721 cgggccaacc tgcccctatc ataaaaacgt tcgatgataa ttttgttttt gcagctctaa
     3781 aaataaccga caaaacttat ttgcctaaca cgggtgctta tgttaaattg ctaaaggttg
     3841 acgcaaatgg cgatacaatt tacacgcgag gatatggtgc gccaagtggc cgcgatacat
     3901 acttaggcaa tttaattcag tcaaaagaca ccggttattt aataacagga aattttgaag
     3961 gcaaaccatt ttcatttggt ggttcttatt tatttaaaac agacacaggc ggttacacac
     4021 catgcggagg catgtacgat gagccattga atatgacacc tctttttttt accgatagtt
     4081 taatgtctct atcaagtata gatggtgccg atgtattacc agcatttgct acacctctat
     4141 tttctacaag tgtaataaca gccgatgatt gtattgtgct tgcaaaccca actacttcac
     4201 ctcagtcatt gtattcgtgg ccatatccta atccaactaa aggagaagtc aatattcaaa
     4261 aatcgaacaa tgttccattg agttatatta cagtttacaa ccatcaaggc atacttgttt
     4321 tgcaaaagaa actggaacaa gacgaagaag gaaaaattga cctatcaaaa tttggccccg
     4381 gcttatacct gataaaaata aacgagcaag gcaaaataag aacagagcag gtggtggtgg
     4441 agtgagcttg gtttctgaat aattcactgt gagtgtatac gaaggaggat tgattccaat
     4501 atcaatccta cttctttttt attaaagccc aagagcagca tcccattttt taacatttgt
     4561 cgttatacta cagccccgct tttcatcgtt acaaccataa cgattattaa tttcctgtat
     4621 ttgtttttat aaggataaac aatcccttat agtgtagtaa gattttccgg agttggtatt
     4681 tatttctgtt ttattactct cattgaatca atcaatatga gccggcttga tattaaaatt
     4741 ccttctatca ttataatttt ttaaaacata aaaatatgag taccacaaaa ttgccaccgc
     4801 gccaaaagat gatcaacatg atgtatcttg tccttacagc tatgctagca ttgaatattt
     4861 cgaaagaggt aatcaatgct tttgttactg taaatacgag tctcgaaaaa agtttcgaca
     4921 accaaaaagt aaaaaagcaa cgcatgttcg aattgtttaa acaacaaatg caacacgatt
     4981 cgctgcgggc gcaactggca tatagcaagg ctttggtcgt ttcacaacaa agcgataagg
     5041 tatgtgccaa catagaaaca ctcaaaaaca gattgataaa cgaaaccgaa ggggtaaaac
     5101 aagccgcatc atttatatcg ttgcaagatg taaagcgcaa agatgcttac gaaaaagtaa
     5161 cgaatattat gtgcggtgac gatgagcaag gccgcggtgc cgaagccaca aagctgaaac
     5221 aaaatatttt gaattataag caaagtgtac tcactacact tgacgaaaaa gatcacatca
     5281 tttttaaacc attattagat aaaaattttg atacaagtga tcccgctaaa gatgatgtgg
     5341 cttacaagga agatcacaaa actacatggg aaatgaaata cttttatcaa acacctgtgg
     5401 tggcatcggt tgcaatgctt agcaaaattc agactgatgt aaaaaatgcc gaggccgaag
     5461 ttcttgaaca tttactaaat ggaataactg ccgataaaaa tactttcgat aaactggtag
     5521 cacaggtggt aatgcctacc gattatattt ttaccggtca acccgtaaaa gcagatatat
     5581 ttcttacagc atacaactca accctgagca gcgatgtgaa tgtaaacgga caacaaatta
     5641 ctat
//
LOCUS       DAORQA010000106         5623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3668_length_5623_cov_7.809267, whole genome shotgun sequence.
ACCESSION   DAORQA010000106 DAORQA010000000
VERSION     DAORQA010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3668_length_5623_cov_7.809267"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            577..1497
                     /locus_tag="PK736_06315"
     CDS             577..1497
                     /locus_tag="PK736_06315"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRC33040.1"
                     /translation="MRNKLFIVLVLITIPTLLFSQRRTKYKSKRWKSYRYEFDMGIGG
                     TNFLGDLGGANQIGTNYFKDLEFSMTRLAVSAGMRYRLSPTIAIHPHIMYGKVAGDDK
                     LTTEFFRSYRNLNFKSNIWEVNCNVEAFILKEQLGRKYKLRGVKGRKSFEVGMYLFAG
                     IGAFRFNPKGEYEGAWYKLRPLHTEGQTFVETRKNYSRVQLCIPVGIGFKYNFDRRWG
                     MTLEYGIRKTFTDYIDDVSKTYFDKNTLLSQENGALAYALSDKSDNSYPGSTGAGQQR
                     GDPRDKDAYMFAVFSVTYKLRTGRVNYPIF"
     gene            1541..2389
                     /locus_tag="PK736_06320"
     CDS             1541..2389
                     /locus_tag="PK736_06320"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRC33041.1"
                     /translation="MKRFAFACFLILALSGFNSAQSQSSELCVFGGIASTKGDINRTL
                     FQARFFNPAYGLRFRHCYNNHWAINISATQGKVEGDDLYSGSAYQRYRNLRFFSNVTE
                     VGLNLEFNFFKFQTANPKTHATPYLFVGYNGFYFNPKAELNGVIYKLQPLGTEGQGTS
                     GLPNSKKYNRLSRSFGFGGGAKFRLGRRFGLVLESGARKTSTDFLDDVSGNYASKPSL
                     AAENGAAAALLSDPSIDQGNNNNYLRQRGNINDNDWYFFGGVSITFTLSKNYHNVCRP
                     FKIQLH"
     gene            complement(2863..5538)
                     /locus_tag="PK736_06325"
     CDS             complement(2863..5538)
                     /locus_tag="PK736_06325"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRC33042.1"
                     /translation="MKLKNNMPQCIALCIVAMLTFAACKSTKDVQSNSNAKSTNDTTS
                     QKIVAPKIYRASNTIINDLVHTKLQVSFDWNKKHLYGKATITCKPHFYTTRSLTLDAR
                     GMDIKEVSMIGNNTKSKLNFAYLNDTLLIDLDRDYTRNETYTIYVDYIARPDELKNKS
                     TGVAITDDKGLYFVNADGKDKSKPKQLWTQGETESSSVWFPTIDKPNMKTTQEIYITV
                     DSNFVTLSNGALMSQQNNGDGTRTDYWKMSKPHAPYLFMMAVSEFAIVQDKWRNIPVN
                     YYVDKEYEQYAKKIFGATPQMLEFFSTRLGVDYAWEKYSQVIAHDYVSGAMENTSATL
                     HGEFVQTDSRQLLDNDQEDIIAHELFHQWFGDLVTCESWSNTPLNESFATYGEHLWRE
                     FKDGRDAGDDVGVGDLRAYLNESGRKQVNLIRFEYEVRDNMFDSHSYAKGGRVLHMLR
                     KYVGDDAFFASLKEYLETNKYQAVEIHNLRLAFEKVTGEDLNWFFNQWFLDKGHPVLD
                     ITYNYVDSNHMQQITVKQLQDFSTTPLYKLPVDVDLYINGKAERHRITITKSEETFSV
                     FSKTKPDLINFDAEKMLLCRKTDNHSPEEWQYMYYHAPLLVDRYEPLDQIGKNYKPGT
                     PQAQIVLDALNDKYSGVRIKGIKNSSTFAKKDETKSLIKSKLSELALKDAESDVRAAA
                     IEALAASFTDTSLVPLYNNCLRDSSYTVVKQALNGYVKCDKAGALNAATYLVKQNNKI
                     INRSVANVYAEFGNESHAEFLLKSFTETNGFAKYSLLQKYSEYLKRMPVNIIEKAVPA
                     IQAMAQGKSAWFVKLAATNTLNDISKKLKTKGDELEKEIKTLKADNQNSALVISQALE
                     FDKTQRLQQFIERAIGDIKANETDEQLIKAYKELK"
BASE COUNT         1636 a         1054 c         1112 g         1821 t
ORIGIN      
        1 atagagccgt aggctcgact ccattttgta aggatggatt tcaatccatc ctattaaaaa
       61 acaaaagagc agaaaggaag cattatttta tagcgcgcta cttaccttct actcagttgt
      121 caccttaact tatattgttg ctcataatct attctaattt cataatactg aatttttaat
      181 gctgcttttt atttgttgtg tttcaaaaaa ttagcagctg gtcaatgtta tatcttctta
      241 gtggatgtaa atttacgcca tcgaaaatgg ccgggtgtca cgccattgta actatatgta
      301 atgctattgt aaggtatgta acaaaagggt aatttattaa tgaaaaggca agagggttaa
      361 ttcaaaaagg aagaggagta agtcaaaaaa gccattattt attgttgatt aaacaaaaaa
      421 agcaaatgag gactgtctag tggggtaaat taaaatggaa gctcgaaaag aggcaaagcc
      481 catgaaaagc aatgaacatg ctgttaaaaa tttaacttaa aatttgtggt taaggcaaat
      541 aaaattattt ttgtcctccc aattaagagg aaaaatatga ggaataaact atttattgtt
      601 ttagtgctaa taactatacc cactctattg ttttcgcaaa ggcgcaccaa atataagagc
      661 aagcgatgga aatcgtaccg ctatgagttt gacatgggca taggaggcac caactttttg
      721 ggcgatttgg gtggagcaaa tcaaataggc accaattatt tcaaagattt agagttttcg
      781 atgacacgcc tggcggtgag tgcaggtatg cgttaccgtt tatcgccaac cattgctatt
      841 catcctcata taatgtatgg caaagtagcg ggcgatgata aactaactac agaatttttt
      901 cgcagctatc gcaacctgaa ttttaaatcc aatatttggg aggtaaattg caatgtcgaa
      961 gcatttatat taaaagaaca acttggccgt aagtataaac tacgtggtgt aaaagggcgt
     1021 aaatcattcg aagtgggtat gtatcttttt gccggaattg gggctttccg tttcaatccc
     1081 aaaggggaat acgaaggcgc atggtataaa ttgcggccat tacacaccga ggggcaaacc
     1141 tttgtagaaa cacgtaaaaa ttatagccgt gttcaattgt gcattcctgt agggattggt
     1201 tttaaatata acttcgaccg cagatggggt atgacccttg aatacggtat acgcaaaaca
     1261 tttaccgatt atattgacga tgtaagcaaa acgtatttcg ataagaatac tttactttct
     1321 caggaaaatg gggctttagc ttatgcatta agtgacaagt cggacaatag ctacccgggc
     1381 tcaacaggtg caggtcagca acgtggcgac ccgcgcgata aagatgcgta catgtttgca
     1441 gtgtttagtg taacctataa actacgcaca ggcagggtta attatccaat tttttaaccg
     1501 caaaataaac catcaacaat ttacgttgtt ttcaacctca atgaaaaggt tcgcgtttgc
     1561 atgtttttta attttagcct tatcaggttt taacagtgca caatcgcaaa gttccgaatt
     1621 gtgcgtgttt ggcggcattg cttcaaccaa gggcgacata aaccgcacct tgtttcaggc
     1681 acggtttttc aatccagctt atggattgag gttccggcac tgctataaca atcattgggc
     1741 aataaatatt tcggctacac aaggaaaagt agaaggtgac gatttgtatt caggttcggc
     1801 ttatcagcgg tatcgtaatt tacggttttt ttcgaatgtt accgaagtag gactaaatct
     1861 ggaatttaat ttttttaagt ttcaaactgc aaatccaaaa acgcatgcta cgccttattt
     1921 gtttgtgggt tacaatggtt tttattttaa tcccaaagca gaactaaatg gagtaattta
     1981 taaactacag cctttgggaa ccgaagggca aggaacatcg ggcttgccca attcaaaaaa
     2041 atataatcgc ctttcacgct catttggttt tggcggtggt gcaaaattca gactcggcag
     2101 acgatttggc cttgtgcttg aaagtggtgc gcgcaaaact tcaaccgatt tccttgacga
     2161 tgtaagtggc aactatgcca gtaagccttc attggcagcc gaaaatggtg ctgcggctgc
     2221 cttattaagt gaccctagta tagaccaggg taacaacaat aattatttgc gccaacgggg
     2281 caatataaat gataacgact ggtatttttt tggtggtgtt tctatcacct tcacactatc
     2341 gaaaaactat cataacgttt gccggccttt caaaatacaa ttgcactaat tgtggattga
     2401 caaacgagat tgttgcagtt tatttcttca tctgtaactg ttacaacttt acataataaa
     2461 acaaccttac tattactgta tacgcaggta ttgcattttt tgaaaaaaat aatgcaaata
     2521 aaattgtctt caaaccatag aacaaaaaac tttctcacaa tttttacacc caccttggcg
     2581 cttccattat caattacaat aaagtaaaag ccatgcggta aattttgaag aaacgaaata
     2641 tggtgatgat actcgccagc cgcttgaaat ttattgttga gtaaagtttt aatttcactt
     2701 ccggccatgt ccaataattt tatggtgatg ttatggcctc agttaatgta tatgttagtg
     2761 tagcattttt aagttgaatc taaactaatt tcaaattatg agacgaaaaa aaaaaattaa
     2821 atagaactag aaagtgacac aaggcgattt ataatggaat gattatttca actctttgta
     2881 tgcttttatc aattgttcat cggtttcgtt tgctttaata tcgccaattg ctcgctcaat
     2941 aaattgttgc aggcgttgtg ttttatcaaa ctccaatgcc tgacttatta ccaatgcaga
     3001 attttgattg tcggctttca atgtttttat ttctttttca agttcgtcac cttttgtttt
     3061 tagttttttt gaaatgtcat tcaaagtatt ggtggcagca agttttacaa accatgccga
     3121 tttgccttgt gccattgcct gaattgccgg cacggctttt tcgataatat ttaccggcat
     3181 gcgtttgagg tattcgctgt acttttgcag taaactatat tttgcaaatc catttgtttc
     3241 ggtgaaagat ttcaataaaa attcggcatg gctttcattg ccaaattcgg cataaacgtt
     3301 ggccacactg cggtttataa ttttattgtt ttgttttact aaataggttg ccgcatttaa
     3361 tgcaccggct ttgtcgcatt ttacatagcc atttagtgcc tgcttcacta ccgtgtacga
     3421 actatcgcgc aggcaattgt tatacaatgg cacaaggctg gtatcggtga atgatgctgc
     3481 aagtgcttca atggccgcag cgcgcacatc ggactcagca tcttttaggg cgagctcgct
     3541 gagtttgctt tttatcagcg atttagtttc atctttcttt gcaaaagtgc tgctgttttt
     3601 aattccttta atacgaactc ccgaatattt gtcgttcaat gcatcgagta caatttgtgc
     3661 ttgaggtgtt ccgggtttat agttttttcc tatttgatcg aggggctcgt agcggtcgac
     3721 taatagggga gcatgataat acatgtactg ccattcttcg ggcgagtggt tgtccgtttt
     3781 gcggcacagc aacatttttt ccgcatcgaa gtttatcagg tcgggtttgg ttttcgaaaa
     3841 aacagaaaag gtttcttcac tttttgtaat ggtgatacgg tgacgttcgg ctttgccatt
     3901 tatatacaaa tcaacatcaa caggtaattt gtaaagtggt gttgtcgaaa aatcctgaag
     3961 ttgtttcacc gttatttgct gcatgtgatt ggagtcaaca tagttgtagg tgatgtcgag
     4021 caccggatgt cccttatcta aaaaccactg attgaaaaac caatttaaat cttcgcccgt
     4081 tactttttcg aatgccaggc gaaggttatg aatttccaca gcctgatatt tattggtttc
     4141 taaatattct tttagcgagg caaaaaaagc atcatcgcct acatacttgc gcaacatatg
     4201 tagtacacgt ccacccttgg catacgagtg actatcaaac atattatcac gtacctcata
     4261 ctcgaaccta ataagattta cctgcttgcg tccactctca tttaagtaag cacgcaaatc
     4321 gccaacaccc acatcatcac cggcatcgcg cccatcttta aactcacgcc agaggtgttc
     4381 gccataggtt gcaaacgatt cgttcaatgg agtgttgctc cagctttcgc aagtaacaag
     4441 gtcgccaaac cactgatgaa aaagttcatg cgcaataata tcttcttgat cattatctaa
     4501 taattgtcgc gaatcggttt gcacaaattc tccgtgcaat gttgctgagg tgttttccat
     4561 ggcaccactc acatagtcgt gggcaattac ctgactatac ttttcccaag cgtaatcaac
     4621 tcccaaacgt gtagagaaaa attccagcat ctgtggtgtg gcaccaaata ttttttttgc
     4681 atactgttca tactccttat ctacataata atttacaggt atgttgcgcc acttatcctg
     4741 cactatagca aattcgctta ctgccatcat aaacaaatat ggagcatggg gcttgctcat
     4801 tttccaataa tcggtgcggg taccatcacc attattttgc tggctcataa gtgcgccatt
     4861 actcagcgtt acaaaattgc tatcaaccgt tatgtaaatt tcttgggttg ttttcatatt
     4921 gggtttatca atagtaggaa accaaaccga gctcgattca gtttcgccct gcgtccacaa
     4981 ttgctttggt ttgcttttgt ctttgccatc ggcatttaca aaatacaaac ccttgtcatc
     5041 tgttatggct acaccggtcg atttgttttt taactcatcg ggcctggcaa tgtaatctac
     5101 gtatatggtg tatgtttcgt tgcgtgtata atcgcggtca aggtcaatca acaatgtatc
     5161 gttcaggtaa gcaaaattca atttcgattt tgtattgttg ccaatcatac ttacttcctt
     5221 tatatccatt cctcgtgcat caagcgttaa tgaccgagtt gtataaaaat gtggcttgca
     5281 ggtaatagta gctttaccat acaaatgttt tttattccaa tcgaaactta cctgcaactt
     5341 ggtgtgcacc aaatcgttta ttattgtatt gcttgcgcgg tatatttttg gcgcaactat
     5401 tttttgggat gtagtatcgt ttgtgctttt agcattagaa tttgattgaa catccttggt
     5461 cgatttacat gctgcaaaag ttaacatcgc aacaatgcac aatgctatac attggggcat
     5521 attattttta agtttcattt tgtgaatgag gtaaaattgt gaggtgtaaa tgtgggaaaa
     5581 tttgtggcac taaaaaagtg ctaaatcaaa aggaatgatt aat
//
LOCUS       DAORQA010000107         5514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3748_length_5514_cov_9.643891, whole genome shotgun sequence.
ACCESSION   DAORQA010000107 DAORQA010000000
VERSION     DAORQA010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3748_length_5514_cov_9.643891"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PK736_06330"
     CDS             <1..900
                     /locus_tag="PK736_06330"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRC33043.1"
                     /translation="LASAYSITGNKMAFSQILPPAFSGEVSQTVTGGSFYSPIRDEAI
                     ALNALIEADANNSQIPIMSKHVTDGLKLNRYCSTQEHVFGFLALGKLARNNRNNTATA
                     TVMVNGKPVGNCGVQNAVVNIKDLRGKKVSITSSGQGSIFYYFVSEGLSMSEKFKQED
                     SYMAVRKFFYDRNGNAVSNLSFKQNDLVVIKISIRALQNTYIDNVVITDILPAGFEIE
                     NPRLSATTDYPWIRNMSTPQYMDIRDDRINLFSQVYYSQQDFYYMVRCVTKGTFKMGP
                     VSADAMYNGEYHSYNGAGVVTIY"
     gene            851..3229
                     /gene="pbpC"
                     /locus_tag="PK736_06335"
     CDS             851..3229
                     /gene="pbpC"
                     /locus_tag="PK736_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRC33044.1"
                     /translation="MANTIPTTVPVLLQFINTMSRRKKIFVGSGVFIAALIILFFVCD
                     RISPVQSHVSYSTQIMSSDSVLIHVFLANDDKWRLKTETMEINPFITKTLLYKEDKWF
                     RYHPGINPIAMMRALVNNIFHAKRTSGASTITMQVARLLYPAPRTYKNKFIEMFRAFQ
                     LERHYSKDQILQLYLNLVPYGGNIEGIKSASYIYFQKPPQQLSLSEITALAIIPNRPT
                     SLSKKNTQLLVAERNKWLTRMRNDNLYNKQMIADAMMEPIALSRHRLPTAIPHLASRL
                     KASTNECDIIYSSINRNAQYSVEKIVKNYAAKLSLLDITNAAVLVNDNVTGKIMCYIG
                     SADYNDNINGGQVDGTKAIRSPGSTLKPLVYGMAFDQGLLTPKTVMADVPMNVSGYIP
                     ENYDEKHNGAVTTEFALINSLNIPAVKTLNDVGLQTFIEKLNTCGFSSITQKQNGLGL
                     SMVLGGCGVTLEQLVRMYSAIANEGTLQPMLYTMDVIKDTVSKVKIMSSEASYMVTDI
                     LSQMTRPDLPYNMQNAINKSKVAWKTGTSYGRRDAWCVGYNKRYTVGVWVGNFSGVGV
                     PELSGAEMATPLMVEIMNTLAYNKNIAWYSPPSKIKVRSVCSVTGDLPGEYCTSTSMD
                     FYIPMVSKNQICTHLHNVAVSNDEKYAYCMHCMPAYNNQVKYKLCDNLTAELQTFYDN
                     NNISYLRQPPHSPTCERVFGDTYPLIVSPVNNMEYITQKNDDNQMLLQSQVTADVQKI
                     YWYINNKLYKACSPAERIFFIPEKGTSKITCADDKGRNTTITIKVKSLGGNL"
     gene            3361..5040
                     /gene="hutU"
                     /locus_tag="PK736_06340"
     CDS             3361..5040
                     /gene="hutU"
                     /locus_tag="PK736_06340"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRC33045.1"
                     /translation="MSVTKTEKLDIAELVTPTGTKLNCKGWLQEAALRMLYNNLDPDV
                     AERPEDLIVYGGTGKAARNVEAFHLIVKALKDLNDDETLLIQSGKAVGILPTHVDAPR
                     VIISNSQLVPHWATWDYFRELEDKGLIMYGQMTAGSWIYIGSQGIVQGTYETFGEVGR
                     QHFGGSLHHTLSVTGGLGGMGGAQPLAITMNEGTCLAADVEEWRIRKRLETRYIDEIE
                     FDIDKAIDRAVQYKKEGKALSIGVVCNVVDLLERMIERNITPDVLTDQTSAHDALVGY
                     LPQGVSVEEGKILRETNPQKYILLSKQTMVKHVQLMIELQSKGAVTFDYGNNLRGQAL
                     EMGLKNAFDFPGFVPAYIRPLFCEGKGPFRWAALSGDPQDIYETDKLILELFPENKGL
                     HRWIKMAQERIAFQGLPARICWLGQGEREKAGLAFNKLVADGKVKAPIVIGRDHLDCG
                     SVASPNRETEAMKDGSDAIADWPVLNALINTAGGASWVSLHHGGGVGIGYSIHAGMVI
                     VADGTDAAAKRLKRVLHNDPAMGVIRHLDAGYDIAKDTARAHRLDIRERLS"
BASE COUNT         1739 a         1027 c         1209 g         1539 t
ORIGIN      
        1 cttgcctcag catactctat caccggaaat aaaatggcat tctctcaaat actgcctcct
       61 gcatttagtg gcgaagtatc gcaaactgtt accggtggaa gtttctactc tcctattcgt
      121 gacgaggcaa ttgcactcaa cgcccttata gaagccgatg cgaataacag tcaaattcca
      181 ataatgagta agcatgtaac cgatggactt aaacttaaca ggtattgcag tacacaggaa
      241 catgtttttg gttttcttgc tttgggaaaa cttgcacgca acaatcgcaa taatacggca
      301 actgccacag tgatggtaaa tggcaaacct gttggtaatt gcggtgtgca aaatgccgtt
      361 gtaaatatta aagatttgcg tggtaaaaaa gtctctatca cttcatcggg acaaggaagc
      421 atattttatt atttcgtatc ggaaggtttg agcatgagcg aaaagtttaa gcaagaggat
      481 agttacatgg cagtgcgcaa attcttttat gatagaaatg gaaatgctgt ttcaaattta
      541 tcgttcaagc aaaatgattt ggtggtgata aaaataagca ttcgtgcatt gcaaaatact
      601 tacatcgaca atgtagtaat taccgacatt ctaccggcag gattcgaaat tgaaaatccc
      661 agactgagcg ccaccaccga ctatccgtgg attagaaaca tgagcacgcc acaatacatg
      721 gatatacgtg acgacagaat aaatcttttt tctcaggtat attatagtca gcaagacttc
      781 tactacatgg tgcgttgtgt tacaaaagga acttttaaaa tgggtcctgt aagtgccgat
      841 gctatgtata atggcgaata ccattcctac aacggtgccg gtgttgttac aatttattaa
      901 taccatgtcg cgtagaaaaa aaatattcgt tggctcaggt gtttttattg ctgcattaat
      961 aattttattt tttgtatgcg atagaatttc gcctgtacaa tcacatgtta gttactccac
     1021 acaaattatg agtagcgata gtgtattgat tcatgtgttt ttggccaatg atgataagtg
     1081 gcgattgaaa accgaaacaa tggaaataaa tccgtttata acaaaaacac ttttgtacaa
     1141 ggaagataaa tggtttcgct accatccggg tattaatcct attgctatga tgcgcgcatt
     1201 ggtgaataat atttttcatg ccaaacgaac ttcgggtgca tctaccatca ccatgcaagt
     1261 tgcacgattg ctatatccgg cacctcgcac atacaaaaat aaatttatag aaatgtttcg
     1321 cgcctttcag ttggagaggc actatagcaa agaccaaatt ctgcaactct atttaaattt
     1381 agttccatac ggtggaaata tcgaaggcat aaaatcagct tcgtatatct actttcaaaa
     1441 acctccacag caactgagtt tatccgaaat tactgcgttg gccattattc caaacaggcc
     1501 cacatcgctg agcaaaaaaa atacacaact gcttgttgca gaaagaaaca aatggcttac
     1561 acgtatgcgc aacgataact tgtacaataa gcaaatgatt gccgatgcca tgatggagcc
     1621 aattgcactg tcgaggcaca ggttgccaac tgcaataccg caccttgctt cacggctaaa
     1681 agcaagtact aatgaatgcg atattattta ttcgagcatt aaccgcaatg cgcaatactc
     1741 tgtagaaaaa attgtaaaaa attatgctgc taaattatcg ctgcttgata ttaccaatgc
     1801 cgctgtgctt gttaatgata atgttaccgg aaaaataatg tgctacatag gttcggccga
     1861 ttataacgac aatataaatg gagggcaagt agatggcacc aaggctatac gatcaccggg
     1921 aagcaccctc aaaccacttg tatacggcat ggcattcgac cagggattgc ttacccctaa
     1981 aacagtaatg gccgatgtgc ccatgaatgt gtcaggatac attcctgaaa actatgatga
     2041 aaaacataat ggcgctgtca ccacagaatt tgcattgata aattcgctca acataccggc
     2101 agtaaaaaca ctcaacgatg taggcttaca aacctttatc gaaaaattga acacatgcgg
     2161 ttttagcagt atcactcaaa aacaaaacgg gctgggtctt tcgatggtgt taggtggttg
     2221 tggtgtaaca ctggaacaat tagtgagaat gtatagtgcc attgccaatg aaggcacgct
     2281 gcaacctatg ctatatacga tggatgtgat aaaagatacc gtatcgaagg tgaaaataat
     2341 gagcagtgaa gcatcgtata tggttaccga tattctatcg caaatgacgc ggcccgattt
     2401 gccatacaat atgcaaaatg cgataaacaa aagtaaagtg gcatggaaaa ccggcacatc
     2461 ctatggcagg cgcgatgctt ggtgcgttgg ttacaacaag cgctatacgg taggtgtatg
     2521 ggtaggaaat ttttcgggtg tgggtgtacc tgaattgagt ggtgccgaaa tggcaactcc
     2581 tttaatggta gaaataatga acacgcttgc ttacaataaa aatattgcat ggtatagccc
     2641 tccatcaaaa ataaaagtgc gcagtgtatg ttcagttacg ggcgatttac ccggtgagta
     2701 ttgcacaagc acatcgatgg atttctatat tcccatggtg agtaaaaatc aaatttgcac
     2761 acacttgcat aatgttgctg tcagcaacga tgagaaatat gcttattgta tgcattgcat
     2821 gccggcatat aataatcaag tgaaatataa attgtgcgac aatctcactg ctgagttaca
     2881 aactttttac gacaataata atatttctta tttgcgccag cctccacatt ctccaacatg
     2941 tgaacgtgtg tttggcgata catatccgtt aatagtttcg cctgtaaaca atatggaata
     3001 catcacacaa aaaaatgatg acaaccaaat gctgcttcaa tcgcaagtaa ctgccgatgt
     3061 gcaaaaaata tattggtaca tcaacaacaa actctacaaa gcatgcagcc ctgccgaacg
     3121 tatattcttt ataccagaaa aaggcacaag caaaataacc tgtgccgatg acaaaggccg
     3181 caatacaact attaccatta aagtgaaatc gttgggagga aacttgtaat ttttgtcttt
     3241 gttgcaactt tcccttgatt aagtatcagt atgtccaaaa gggcatacaa aagagaaata
     3301 tttttcgctt agtttgaaac aaagaatagc tatttttgtc aactataaaa aagaaatatt
     3361 atgtcggtaa caaaaactga aaaactggat attgctgaat tggtaacacc tacgggcacc
     3421 aaattaaatt gcaaaggatg gctacaggaa gcagcattgc gtatgttgta caacaacctg
     3481 gatcccgatg tggccgagcg ccccgaagat ttgatagtat atggcggcac cggcaaggca
     3541 gcacgaaacg tggaggcatt tcatttaata gttaaggcac ttaaagattt gaacgatgac
     3601 gaaaccttgc tcatacaatc gggaaaggct gtgggaattt tgcctacgca cgtggatgca
     3661 ccacgggtaa taatttctaa ttcgcagctt gtacctcatt gggctacgtg ggattatttt
     3721 cgtgagttgg aagataaagg cttgataatg tatgggcaaa tgacagcagg ttcgtggata
     3781 tacattggct cgcaaggtat tgtgcaaggc acttacgaga catttggaga agttggtcgt
     3841 caacactttg gtgggtcgtt gcatcatacg ctatcggtta caggcggact cggtggaatg
     3901 ggtggcgcac agccgcttgc cataacgatg aacgagggca cgtgtcttgc tgccgatgtg
     3961 gaagaatggc gaattcgcaa gcgcctcgaa acccgttata ttgacgaaat agaatttgat
     4021 attgataaag ccattgaccg ggcggtgcaa tataaaaaag aaggcaaagc cctttcgata
     4081 ggtgtggtat gcaatgttgt tgatttgctc gaacgcatga ttgaacgcaa tattactcct
     4141 gatgtgctta cggatcaaac atcggcacat gatgcacttg ttggatattt gcctcaaggc
     4201 gtgagtgtgg aagaaggaaa aattttgcgc gaaacaaatc cacagaaata tattttgcta
     4261 tcgaaacaaa cgatggtgaa acacgtgcag ttgatgatag aattgcaaag taaaggcgct
     4321 gtaacttttg actatggaaa taatttgcgc ggacaagctt tggaaatggg cttgaaaaac
     4381 gcttttgatt ttcccggttt tgtgccggca tatattcggc cattattttg cgaaggtaaa
     4441 ggcccgtttc gttgggctgc attgtcgggc gatccacaag atatttatga aaccgataaa
     4501 ttaattcttg aattgtttcc tgaaaataaa ggcttacacc gttggataaa aatggcgcaa
     4561 gagcgaattg catttcaagg attaccagct cgtatatgtt ggcttggcca aggagaacgc
     4621 gaaaaagccg gattggcatt taataaactt gtggccgatg gaaaagtaaa agcaccaatt
     4681 gtaataggtc gcgatcattt ggattgtggt tcggtggcat cgcccaatcg cgaaaccgaa
     4741 gccatgaaag atggaagtga tgccattgcc gattggcctg tgcttaatgc attgatcaat
     4801 actgccggag gtgcaagttg ggtgtcgtta catcatggtg gtggtgtggg tattggctat
     4861 agtattcatg caggtatggt tatagttgcc gatggtaccg atgccgcagc aaagcgcctg
     4921 aaacgagtgt tacacaacga ccctgcaatg ggtgttatac gacacctcga tgccggatac
     4981 gatattgcaa aagacaccgc gcgagctcat cgtttggata tacgagaaag gctttcttag
     5041 atattagttt cggaaaacag tttttagttc tttgttccgt cattgtaagg tacgaagcaa
     5101 tccctgcgta gaactggttt cgtttgcaga gaggattttc tcttatatta atttcagagt
     5161 cactcatgcc aataaaaaac aataaacaaa tgaggactct gaaattcgcc tgattcaggc
     5221 cgctgtataa tatacaattt gcaaaatatt ttttttggca aaaattggat ttaaaaaatg
     5281 cattctattt tagcaaatgc tgtgagaagt atatatactt tgtgttacta aatgcttctt
     5341 gaaaagtgaa gcaagaaaaa atcaaggctt tgaaaccgat ggatttttag atgaaagcaa
     5401 tgcgtatttt tttgccatgg ctttgttgcc aactatagta taggctgttt gtaaactatc
     5461 gtataccatt ttattttcgg ggtatagatt ttcgttgtac ttaaaaattt cgat
//
LOCUS       DAORQA010000108         5500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3760_length_5500_cov_7.720110, whole genome shotgun sequence.
ACCESSION   DAORQA010000108 DAORQA010000000
VERSION     DAORQA010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3760_length_5500_cov_7.720110"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1818)
                     /locus_tag="PK736_06345"
     CDS             complement(103..1818)
                     /locus_tag="PK736_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC33046.1"
                     /translation="MITKVTINNFKKLERISFPLSQSVVVIGPNNSGKSTIFQALCLW
                     EIGVTNFIQAHQRKDLGRNGSVTINRKDLLNSPIADARFLWRNRKVTEKNEQGQTKHV
                     KLHVELQGEELGKNWVCKAEFEFSNAESFQCRIISGSKEITQLYNSGAGIHFGFLQPM
                     SGISTNEDKLTQGSIERKLGEGKTAEVLRNICYEILYPDTPHPKGYNPEEKWLKLCKG
                     VKSMFGADLLKPEFIKATGLIQLEYTEDKIRYDISSGGRGFQQTLLLLSYMYANPNTI
                     LLLDEPDAHLEVIRQREAFQLINQVANDMNSQILIASHSEVVLDEAAEASKIIALIEN
                     KVFELNESSSPQRIRYIKKALTEIGWEKYYLARAKRHVLYLEGSTDLQMLLHFANKLN
                     HKVEPLLRVANVQYTADNVPVTAINNFASLQEIFSDLKGLALFDNLPNLQDNPKLKII
                     CWTRRELENYFAQPEMLYRHAKLLAAKYPSIGSAKLVDTMKQAVQDSTVPLYLKDLKN
                     DWWHKAKLSDEWLDVIFPEFYKKLGVYNDFSKRDYYQLIMLMKPDEINKEISEKLDNI
                     FGLLS"
     gene            complement(2084..2713)
                     /locus_tag="PK736_06350"
     CDS             complement(2084..2713)
                     /locus_tag="PK736_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC33047.1"
                     /translation="MASITLSGITKKFGKHIVFRNITTTIYDGEGVAIIGSNGSGKST
                     LLKVIAGYVEPSAGKVEYAIANLQVNTNNCYQHLSYAAPYMDLIEEFTLSEQVNFYFK
                     FKEMNSNLKSIEEFVSKCGLEQAKEKQIKYFSSGMKQRLKLSLAIMSNSSYLFLDEPL
                     TNLDAQGTVWYQSLMTEYTGNRIVIIGSNSMKDEIFLCRKNIDIEEWKN"
     gene            complement(2725..3516)
                     /gene="lpxA"
                     /locus_tag="PK736_06355"
     CDS             complement(2725..3516)
                     /gene="lpxA"
                     /locus_tag="PK736_06355"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRC33048.1"
                     /translation="MISPLAVVSSKATIGKNVSIGPFTLVHDDVVIGDNTKIENSVTI
                     FDGARIGAGCHIFPGAVISAVPQDLKYTGEITFAEIGDNTIIREFVTVNKGTIAAGKT
                     VLGKNNLLMAYTHVAHDCMIGNHCILANGVTLAGHITIDDYAIIGGLCAVHQFVHIGS
                     HVMISGGSLVRKDVPPYTSSAHEPLAYIGVNSVGLRRRGFTNEEIAEIQNIYRYIFVE
                     NHTVPKALEMVEAQCAAGPHRNHIVDFIRKATRGIMKGYMPQLDN"
     gene            complement(3528..4970)
                     /locus_tag="PK736_06360"
     CDS             complement(3528..4970)
                     /locus_tag="PK736_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HRC33049.1"
                     /translation="MELTAETSTVANVTQGEGLVQTTLKNSVTISGVGLHTGKQCTVT
                     ILPAAENYGYRFRRTDLPGNPEVQADVDNVTDTARGTTLTQNGASVNTTEHLLSALAG
                     LEIDNALIEITGPELPIMDGSAHAFVEALQKVGVQQQKAERKYYVLDETLTYEDPTRK
                     VEMLAVPSADFRVTVMVDYDNDVLGTQHAQIHSMREFASEISRCRTFVFLHELMPLLK
                     NNLIKGGDLNNAIVLVSQDIPDADMDHLRKVFNKPDIKVEGKGVLNHIAMHYMNEPAR
                     HKLLDIVGDFALVGTPLKAHILAARPGHKANVEFAAKIKAHIKSKRLKSVVRTFDLNK
                     PAVYDINKISSILPHRFPFLLVDKILELTDTRVVGLKNVTMNEWFFEGHFPGNPIMPG
                     VIQIEAMEQVGGILALSTVPDPENYLTYFMKIDHAKFKQKVLPGDTIIFDLELLQPIR
                     RGIILMKGCAYVGDKVVLEAEMMASIVKKN"
BASE COUNT         1529 a         1125 c         1009 g         1837 t
ORIGIN      
        1 gtataaaata ttcattttct tcaatttatt aatgaaacaa tgtgcctttc taatcttatg
       61 tttatgctgt tccgctatct tttttattaa ttctattcaa ttctagctca acaatccaaa
      121 tatattatca agcttctctg aaatttcttt attgatttca tcgggcttca taagcattat
      181 aagttggtaa taatctcttt tagaaaagtc attatacacg ccaagttttt tatagaactc
      241 tggaaagata acatcaagcc attcgtctga taactttgct ttgtgccacc aatcgttttt
      301 caaatctttc aagtaaagtg gaacggttga atcttgaaca gcttgcttca tggtgtcaac
      361 aagtttagcg gaaccaattg aaggatattt cgctgcaaga agttttgcat ggcgatatag
      421 catttcgggt tgagcaaaat aattttcaag ttctcttctt gtccaacaga taatttttag
      481 tttaggattg tcttgaagat ttggcagatt gtcaaaaaga gctaaccctt ttaagtcgga
      541 aaaaatttct tgtagtgaag caaaattatt gattgccgtt actggaacat tgtcggcagt
      601 gtattgaaca ttagcaactc ttagcaaggg ttcaacttta tgatttagtt tgttggcaaa
      661 gtgcagaagc atttgtaagt cagtagaacc ttcaagataa agaacatgcc tttttgctcg
      721 ggctaaatag tatttctccc aacctatttc agttaatgcc tttttgatgt aacggattct
      781 ctgtggggaa gaagattcat ttaattcaaa aactttattc tcaatcaaag cgataatctt
      841 tgatgcttcg gctgcttcgt ctaaaactac ttctgaatgg gaagcaatta gaatttgaga
      901 attcatgtca ttagcaactt ggttgatgag ttgaaatgct tctcgctgtc tgataacttc
      961 aaggtgagca tcgggttcgt caagtaacaa aattgtattg gggtttgcat acatgtatga
     1021 aagtaaaagt aatgtctgct gaaaacctct accgcctgat gaaatatcgt aacggatttt
     1081 gtcctctgtg tattccaatt gaattaaccc tgttgcttta ataaactctg gtttcaataa
     1141 gtccgcacca aacattgact taacaccttt gcagagtttt aaccactttt cttctgggtt
     1201 atatcctttt ggatgtggtg tgtcaggata aaggatttcg taacaaatat ttcttaggac
     1261 ttcggctgtt tttccctcac caagttttcg ttcaattgaa ccttgagtaa gtttgtcctc
     1321 attggtagaa atacccgaca ttggttggag aaagccaaag tgaattcctg cacccgaatt
     1381 ataaagttga gtaatttctt ttgaaccgct tataattcta cattgaaatg attccgcatt
     1441 tgaaaattca aattctgctt tacaaaccca attttttccc aactcttcac cttgcaattc
     1501 cacgtgtagc ttgacatgct tggtttgtcc ttgctcgttc ttctctgtta ctttccggtt
     1561 tctccataaa aatcttgcgt ctgcgattgg gctattcaat aaatctttgc ggttgattgt
     1621 aacagaacca tttcttccta aatcctttcg ttggtgtgct tgaataaaat tcgtcacgcc
     1681 aatttcccac aagcacaaag cttgaaatat tgttgacttg ccagagttat ttggtccaat
     1741 aacaacaact gattgagaaa gtggaaagga aatcctctcc agttttttga aattgtttat
     1801 cgttacttta gttatcattt tatattcttt gtgttttcgg actgcaattt agagtttaat
     1861 cggttgcaaa aatatgattc tatccgttca tttttgtatt gaaaaactgt tggttcaaag
     1921 ttggtgagtt gtaatttgca ttgctgcttt taaactgtgc ccttttacac caaaagttag
     1981 caggtttttg aaaaaacctg cttacttctg gtaatgccga acaacttcaa aagaaaaatt
     2041 ttccaaaacc taagttgctt cggtataagt aaattatgca cctttaattt ttccattcct
     2101 ctatatctat atttttcctg cacaggaata tttcatcctt catactgttc gatcctataa
     2161 ttacaattcg gttaccggta tattctgtca ttaacgattg ataccacacc gtgccttgtg
     2221 catcgagatt ggtgagtggt tcatctaaaa aaagatagga cgaattgctc ataatagcca
     2281 acgacaattt caagcgttgt ttcattcccg atgaaaaata ttttatctgc ttttctttcg
     2341 cctgttccaa tccacatttg cttacaaact cttctatcga ttttaaattg ctattcattt
     2401 ctttaaactt aaaataaaaa tttacctgtt cgctcagtgt aaattcttct atcaggtcca
     2461 tgtatggggc agcatagcta aggtgttgat agcagttatt tgtattcacc tgcaggttgg
     2521 caatggcata ttccacctta ccggccgatg gctccacata gccggcaatc actttgagta
     2581 aggtactttt gcccgaacca ttgctgccaa taatggcaac cccttcgcca tcgtatatgg
     2641 tggtggttat gttgcgaaat actatatgct ttccaaattt ttttgtaatg cccgaaagtg
     2701 tgatagatgc caagggtcaa tcagttaatt atctaattgc ggcatatatc ctttcataat
     2761 tccgcgggta gccttgcgaa taaaatcaac aatatgattg cggtgtggcc ctgcagcaca
     2821 ttgcgcttca accatctcaa gtgcttttgg caccgtgtgg ttttcaacaa atatatagcg
     2881 gtaaatattt tgtatttcag caatctcttc gtttgtaaat ccgcgtctgc gcaaccctac
     2941 cgagttaaca cctatatatg caagtggttc gtgggccgat gaggtgtagg gtggcacatc
     3001 tttgcgcaca agcgaacctc cgcttatcat tacatgcgaa ccaatgtgta caaactgatg
     3061 tacggcacac aagccgccaa tgatagcgta atcgtcaatg gtaatatgtc cggcaagggt
     3121 aacgccatta gcaagtatac aatggttacc tatcatgcaa tcgtgtgcaa cgtgtgtata
     3181 agccatcaac aaattatttt ttcccaacac cgttttgccg gcagctattg ttcccttatt
     3241 cacggtaaca aactcgcgaa taatggtgtt gtcgccaatc tctgcaaaag taatttcacc
     3301 cgtatatttc aagtcttgcg gcactgcact tattactgct cccggaaaaa tatgacaacc
     3361 cgccccaata cgggcaccat cgaatatagt aacactattt tctatttttg tgttgtcgcc
     3421 aatcaccaca tcatcgtgca ctaaggtaaa cggtcctata cttacatttt tacctattgt
     3481 tgctttactg cttactactg caaggggtga tatcatgtat attattatta atttttcttt
     3541 actatcgatg ccatcatttc tgcttccaac accactttat cgcctacata tgcgcaacct
     3601 ttcataagaa taattcctct gcgaatgggc tgcaacaatt ccaaatcgaa aataatggtg
     3661 tcgcccggca atactttttg tttgaatttg gcgtgatcaa ttttcataaa ataggtaagg
     3721 taattttcgg gatcgggcac tgtgctaagg gcaagtatac cgcctacctg ttccatggct
     3781 tctatctgaa ttacgcccgg cattatgggg ttgccaggaa agtggccttc gaaaaaccac
     3841 tcgttcatgg ttacattttt gagcccaaca actcgtgtgt cggtaagctc caaaatttta
     3901 tcgaccaata aaaacggaaa acgatgcggc agtattgatg atattttatt tatatcgtac
     3961 acagccggtt tattcaaatc gaaagtgcgc actacgcttt tcaatcgttt cgattttatg
     4021 tgggccttta tttttgcagc aaattccaca ttggccttat ggcccggacg agccgcaagt
     4081 atatgtgctt tgagcggtgt acccaccaat gcaaaatcgc caactatatc aagcaatttg
     4141 tggcgtgcag gctcattcat atagtgcatg gctatgtgat tcaacacccc tttgccttcc
     4201 acttttatat cgggtttatt aaacactttg cgcaaatggt ccatatctgc atcgggtata
     4261 tcctgactta caagtactat agcgttattc aagtcgcccc cttttatcaa attatttttt
     4321 agcaatggca tcaactcatg caaaaaaaca aatgtacggc agcgcgatat ttccgaggca
     4381 aactcacgca tcgaatgtat ttgtgcatgt tgtgtgccca gcacatcgtt gtcgtaatct
     4441 accataactg ttaccctgaa atcggcactg ggcactgcca gcatttctac cttgcgcgta
     4501 ggatcttcgt aggtcagcgt ttcgtctagc acataatatt tgcgctccgc cttttgttgc
     4561 tgcacgccca ccttttgcaa cgcctctaca aaagcatgcg cactgccatc cattattggc
     4621 aactcggggc cggtaatttc tataagggca ttatctattt cgaggccggc caatgccgac
     4681 agtaaatgct cggtagtatt tacactcgca ccattttgtg taagggtggt gccacgtgcc
     4741 gtatcggtta cattgtctac atcggcttgc acttcggggt tgccgggcag gtcggttctg
     4801 cgaaaacggt agccataatt ttcggctgca ggcaatatcg taacggtgca ttgcttgccg
     4861 gtgtgaaggc ctaccccact tatggttacg ctgtttttta atgtggtttg caccaatccc
     4921 tcaccttgtg taacatttgc tacggtagaa gtttctgctg ttaattccat tttattcctt
     4981 ttgggtgcgc aaagataatc ttttgtttca acgtaaaaag cggtgatatg cttacaatta
     5041 tgtgtgttta ttaaaattca aaactgatgc aataaaagca gacaggcaaa aatgaaaata
     5101 ctttttattg cgggggctgg ttagccctcc gtttgcaaat ggctaaagcc atttgcaata
     5161 aaatacgctc cgcatgttgc cccattactt tcttctttta cacaaattga aaattcggtt
     5221 ttatcaaacc tgcaaaacaa aaaaacaatt taagttggta tgccattctc aaattaaatt
     5281 gagcattaca tgcgaataaa aaaattctca tagattgcgg cataataata tttttacatt
     5341 tgctgcacgt ttattctcaa cctataaata caaaatgtaa aatacaatgt ggatatacgc
     5401 aactgatttt ttttagacaa actaatcgct aaactattga tagcagagga tttgagaaat
     5461 tttgatatta gtaataattc ataacatagt aaactaaaca
//
LOCUS       DAORQA010000109         5493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3765_length_5493_cov_6.096727, whole genome shotgun sequence.
ACCESSION   DAORQA010000109 DAORQA010000000
VERSION     DAORQA010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3765_length_5493_cov_6.096727"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..956
                     /locus_tag="PK736_06365"
     CDS             186..956
                     /locus_tag="PK736_06365"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRC33050.1"
                     /translation="MFTVLPKHKIVLYFITVMIMASCGVNNNLTQTLANPKGITKLTM
                     RNNGIKVLPPGVVKLKELKELYLFNTELDSLPYNIGELINLEKLVVSSNKLTSIPASI
                     GNLKKLKVLSLKNNHITALPSEIGNLTELKELDLQNNDLTILPLSICNLTKLVFLNLH
                     DNMIKELPNDIGNMKLLRFFTIGKNNLTELPDAIGNMSALVELNVAFCGPLVNLPSTL
                     NRCRNLEFIYIDNTIIVPYGITRGNPRLRIVNKSFSEM"
     gene            1082..2899
                     /locus_tag="PK736_06370"
     CDS             1082..2899
                     /locus_tag="PK736_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33051.1"
                     /translation="MKKIYFGFISILVCNFLYASTLLVPSQFATIQSAINASVVGDTV
                     LVDAGATYIENINFMGKDIVVRTPNPATDKYTTFIDGNGGGSVALFISNETNAALLEG
                     FTLKNGTGTLRSWWYYLGFDLIAGGGILCDSPSAFNQAANSAGSSPTLAHLIVENNIA
                     DFGGGIACWRTSRPIIKNTIVRNNTATGVGGGLSFFEGFNTIAGPANLMENVEVTNNN
                     CTGGVGGGGIITNYSSKVVMVHCTVTQNTSTVAAGENLFRANGAFFVMHNSIIWTPNT
                     DVLYDQGGTPVQSTIDYSCVANFNGPLTNVIGNITTNPDFINPGANDFHLMATSLCIN
                     AGNLVNSSPIDLDSNLRDVQPDMGAYEIVSLAPAPAANLQSSDTSFCDKNAIDFFDLS
                     TNNPTSWFWTFAGASPSTSTLQNPTGIYYPSYGSFDVTLVACNANGCDTIFLPGFITE
                     YPLPNPPVVSQSNDTLFSTPASTYQWFLIPNPIPGANAQFYVPTQPGSYYVLVSDSNG
                     CEVPSNILVVTNTGFQNNNNGTQNTLNNIGDGIYQLNGSLFSKSDIQIIVTDVLGKIV
                     FQSMNNSIGTEINLQHFAKGIYHANIICNKISFTQRIVR"
     gene            3107..4666
                     /locus_tag="PK736_06375"
     CDS             3107..4666
                     /locus_tag="PK736_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRC33052.1"
                     /translation="MINTFPYSEDFEANNGGWYLGGTFIDWQWGTPNKAVISGAASGV
                     NCWVIGGLTASFYNYDERSTCTSPCFDFTNIVLPYVTFNIFWESEKKYDGATFQYSLN
                     QGSTWFNVGKFGDPVDCFNDNWFNEQSLTNLNFLASIKHGWSGNIQPTVGSCIGGAGS
                     AGWITARHTMPYLSGKDSVIFRFAFGSGSACNDFDGFAFDDVWIGETPVLFSFTPAIA
                     IDTCSQGKGQISVAVAGGLPPYSYQWSNGNTTGNSISNLFAGTYTVTVSDVSGCTASQ
                     SFIVGFSDNASIGIASVDDTCNAGKGTAMAIANSGTAPFTFTWTGFSSTFDTLNNISA
                     GTYQAFVYDSLGCIDSTYFTIGNINNLTFEIDSIITFCFGIPASVDIGVFDSYLWSNG
                     NTTAQLTIENEGTYIVTVTNQQGCMAEDSLVAVLDCDAGMIVPNTFTPNDDNTNEIFL
                     PQSTFLESYNLKVFNRFGTLIFETSDLKQGWNGTYQDQACPTGVYGYFITYKRYNHDK
                     ETKCGLVRILK"
     assembly_gap    4830..4882
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4883..5074
                     /locus_tag="PK736_06380"
     CDS             <4883..5074
                     /locus_tag="PK736_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33053.1"
                     /translation="NNCHWPIFINAIANEAKQSLHRPKAFVIANEVKQSLQRPEAFVI
                     ANAVKQSLQQPHHSSLQTK"
     gene            complement(5204..>5493)
                     /locus_tag="PK736_06385"
     CDS             complement(5204..>5493)
                     /locus_tag="PK736_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRC33054.1"
                     /translation="RCIVVEGVRVMSEAVLGANVVLTMSTKIIDVAGTEPIETKGIVP
                     SRSVVIPGSYTKKFAAGDYQVPCALIIGKRKESTDTKTSLNNALRDFNVAV"
BASE COUNT         1586 a         1078 c         1041 g         1735 t
ORIGIN      
        1 tagtttatta gagaacaatt gaaacatttc gaagaagaaa attattaaaa tgtaatttag
       61 ttagagaaat aaaaatgagc cgccccgcaa ttgtgaggcg gcttttttat ttttataaat
      121 ctcagtttta ttttatctat ctttatcaca ccaatcattg tttcgaaaaa agaaattgaa
      181 aattgatgtt caccgtttta cctaaacata aaattgtttt atattttatc acagtaatga
      241 taatggcaag ttgcggtgtg aataataatt tgacacaaac attggccaat cccaaaggta
      301 ttaccaaact aacaatgcgc aacaatggta ttaaagtttt acctcctggt gttgtaaaac
      361 taaaagaact aaaagagcta tatttattta atacagaatt agattcgttg ccatacaata
      421 ttggcgaact tatcaattta gaaaaactgg ttgtgagcag caataagtta acttcgattc
      481 cggcttcaat tggcaacctt aaaaaattga aagtgctttc actcaaaaat aatcacatca
      541 ctgcattacc atccgaaata ggaaatctca cggagttgaa ggagctcgat ttgcaaaata
      601 atgatcttac cattttacct ttatcaatct gtaacttaac caaactggta tttttaaatt
      661 tgcacgataa catgataaaa gaattgccca acgatattgg caatatgaaa ctgttgcgct
      721 tttttacaat aggcaaaaac aacctcaccg aattgcccga tgccattgga aacatgagtg
      781 cccttgtaga attgaatgtt gccttttgcg ggccactggt aaatttacct tctactttaa
      841 acagatgccg caacctcgaa tttatataca tcgataacac catcatcgtt ccttatggta
      901 taacccgggg caatccacgt ttacgaatag taaataaatc tttcagcgaa atgtgatttg
      961 ctctttgaaa aatgttgccg cacttcggta ttatttttga cgctgaataa aagttgcgaa
     1021 taattatttt cctaatttcg caataaaata attctatttt tgacaattaa aatttaccct
     1081 tatgaaaaaa atctacttcg gctttatttc cattcttgtt tgcaattttt tgtatgcctc
     1141 aacattattg gtgccatcgc aatttgcaac aattcaatca gcaatcaatg catcggtggt
     1201 tggcgataca gtgcttgtgg atgctggtgc aacttatatt gagaacataa attttatggg
     1261 taaagatata gtggttcgaa caccgaatcc ggctacagat aaatacacca catttattga
     1321 tggtaacgga ggtggcagtg ttgcactttt tatttcgaac gaaaccaatg ccgccctgct
     1381 cgaaggattt actttaaaaa atggcacagg cactttgcgt agttggtggt attatcttgg
     1441 tttcgattta attgccggag gtggtatttt atgcgatagt ccttctgcat ttaatcaagc
     1501 ggcaaattca gccgggtcat cacccaccct tgctcattta atagtagaaa ataatattgc
     1561 tgatttcggt ggtggcattg catgctggcg cacttcgcgt ccaataataa aaaataccat
     1621 tgtgcgcaat aatactgcta caggtgttgg tggtggttta agtttttttg aagggtttaa
     1681 tacaattgca ggcccagcca acttaatgga aaatgtagag gttaccaata acaattgcac
     1741 aggtggagtt ggtggtggtg gcataattac aaactacagt tcgaaagtag taatggtgca
     1801 ttgcacagta acccaaaata cttcgacagt ggcagcgggc gaaaatttat ttcgtgccaa
     1861 tggcgcattt ttcgttatgc acaattctat tatatggact cccaacacag atgtattata
     1921 tgatcaggga ggtacacccg tgcaaagcac aatcgattat tcatgtgtgg caaatttcaa
     1981 tggaccactt acaaatgtaa ttggcaacat aaccactaat cccgatttta ttaatccggg
     2041 ggcaaatgat tttcatttga tggctacaag tttatgtatc aatgcaggta atcttgtaaa
     2101 ctcatcacct atcgatttgg acagcaacct tcgcgatgta caacccgaca tgggcgcata
     2161 cgaaatagtg tcgcttgcac ctgcaccagc tgcaaattta caatcgagcg atacatcatt
     2221 ttgcgataaa aatgccatag atttttttga cctctctacc aataatccta catcgtggtt
     2281 ttggactttt gccggtgcat cgccctccac ttccactttg caaaacccaa caggaattta
     2341 ttatccatcg tatggctcgt tcgatgttac gcttgttgcc tgcaatgcca atggatgtga
     2401 tactattttt ctgcccggtt ttattaccga atatccattg cccaatcccc ctgtagtatc
     2461 tcaaagcaac gatacattgt tctcaactcc ggccagtacc tatcaatggt tcttaattcc
     2521 caatcctata ccgggagcca acgcgcaatt ttacgttcct acacaaccgg gcagttacta
     2581 tgtgttagtt tctgatagca acggttgcga agtgccgagc aatattttag ttgttaccaa
     2641 taccggtttt caaaataaca ataatggcac gcaaaatact ctcaataata ttggtgatgg
     2701 catctatcag ttaaatggtt cactgttttc gaaaagcgat atacaaataa ttgtaaccga
     2761 tgtattggga aaaatagttt ttcaatccat gaacaattca attggaacgg aaataaatct
     2821 tcaacatttt gctaaaggca tttatcacgc gaatataatt tgcaacaaaa tctcctttac
     2881 acagcgaatt gtgcgctagg tttttgattg cattactaag tgataaagca tcaataaaca
     2941 acacaaactt aagtatcatt aaaatgtaaa attcgaatca aattcgtttt actttttatt
     3001 ccttgtatat ttgtgtaaaa ttttcacttt gattcgcatt ttaaaatttc tcattgttag
     3061 cattgttatt tgttgcccga atatttcgaa ggcgcaatgc tcaaccatga taaatacttt
     3121 tccttattcc gaagattttg aagctaacaa tggtggttgg tatttaggcg gtacttttat
     3181 cgactggcaa tggggcacac caaataaagc agtgattagt ggtgctgcca gtggtgttaa
     3241 ctgttgggta attggcgggc tcacggcatc gttttacaat tatgatgagc gttccacatg
     3301 cacgagtccc tgtttcgatt ttaccaatat tgttttgcct tatgttacgt tcaatatttt
     3361 ttgggagtcg gaaaaaaaat atgatggtgc cacatttcag tattcattaa atcaaggtag
     3421 cacatggttc aatgttggta aatttggcga ccctgttgat tgctttaacg ataactggtt
     3481 taacgagcag tcgcttacaa acttaaattt tcttgcaagt attaaacatg gatggagtgg
     3541 aaatattcaa cccactgtag gctcatgcat tggtggtgct ggctcggcag gatggataac
     3601 cgcacgccac accatgccct atttaagtgg caaggacagt gtaattttcc gttttgcttt
     3661 tggctccggt tcagcgtgca atgattttga tggctttgct ttcgatgatg tgtggattgg
     3721 cgaaacaccg gttttgtttt cctttactcc tgccattgca attgatacat gctcgcaagg
     3781 caaaggtcag atttcagttg ctgttgctgg agggcttcct ccctactcct accaatggag
     3841 caatggcaac actaccggca attccatttc gaatttattt gccggcacgt atacagttac
     3901 tgttagcgat gtcagtggtt gcacggcatc gcaatctttt atagtagggt ttagcgataa
     3961 tgcaagtatt ggtattgcat cggttgacga tacatgcaat gcgggcaaag gcacagccat
     4021 ggcaattgcc aacagcggca ctgctccatt cacatttaca tggacaggat tttcatctac
     4081 tttcgatacc cttaacaata tatccgcggg cacctatcag gcattcgttt acgattcgtt
     4141 gggatgcatc gattctactt attttactat tggcaatata aataacctca ccttcgaaat
     4201 cgattcgatt atcacttttt gttttggcat tcctgcatcg gtcgatattg gagtatttga
     4261 ttcttacttg tggagcaatg gaaacaccac agcacaactt actatagaaa atgaaggaac
     4321 gtatattgtt actgttacca atcaacaagg ttgcatggcc gaagatagtt tggtggctgt
     4381 gctcgattgc gatgcaggaa tgattgtgcc caatacattt acacccaacg atgataatac
     4441 caatgaaata tttttaccgc aatctacttt tttggaatcg tacaatttga aagtattcaa
     4501 cagatttggc accctcattt tcgaaacatc cgacttaaaa caaggatgga acggtacata
     4561 ccaggatcaa gcctgcccaa ccggtgtgta cggttatttc attacataca aaagatacaa
     4621 ccacgataaa gaaactaaat gtggattggt gaggatattg aagtagttat tggttattga
     4681 gttgttgtgt tgtttcattg ttgcattgtt gcattgtttg attgttgcgt tgtttgattg
     4741 tttcattgtt gcattgtttg attgttgcat tgtttgattg ttgactttgt cctgctgagg
     4801 tacgaagcat ctctgccaga cattggtcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnaataattg tcattggcca attttcatta acgccattgc
     4921 gaacgaagcg aagcaatctc tgcatcgacc taaggcattc gtcattgcga acgaagtgaa
     4981 gcaatctctg caacgacctg aggcattcgt cattgcgaac gcagtgaagc aatctctgca
     5041 acaacctcat cattcgtcat tgcaaacgaa gtgaagaaat ctctgcaacg acatgagcat
     5101 tcgtaattgc gaacgcagtg aagcaatctc tgcatcgacc tgaccattcg tcatcttaat
     5161 ttccaattct taattcctaa tttccctacc attttcaatt tcatcaaacc gctacattaa
     5221 aatccctcag cgcattattt aaacttgttt ttgtatcggt gctttctttg cgtttgccaa
     5281 taattaaagc acagggtact tgataatcac ctgcggcaaa tttctttgta tagctgcccg
     5341 gtattaccac actgcgcgaa ggcacaatgc ctttagtttc gatgggttcg gtgccggcaa
     5401 catcaattat ttttgtcgac atggtgagta ctacatttgc accaagcact gcttcgctca
     5461 ttacccgcac accttctacc actatgcaac gcg
//
LOCUS       DAORQA010000110         5470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3780_length_5470_cov_7.271283, whole genome shotgun sequence.
ACCESSION   DAORQA010000110 DAORQA010000000
VERSION     DAORQA010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3780_length_5470_cov_7.271283"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2021)
                     /locus_tag="PK736_06390"
     CDS             complement(<1..2021)
                     /locus_tag="PK736_06390"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HRC33055.1"
                     /translation="MKHRIHHQFTLTIFFIICFSKCFSLTLSTDGKAKYTIVISAKAN
                     ENEKHAADVLNKYLFKITGASFLIVDDTHTKSDFEIIVGNTSRNDLKENKINHPDGFS
                     ISTQKNILILNGGTGKGVVYAVYTFLEKYCGCRKYSNDEAVVPKNATLVIPDIIDDVE
                     IPAMDFREYYYTATVDDEYREWHKLMRHFPNDSSSQWGMWVHTFNKLVPEEKYFDSHP
                     EYFSFYGGKRQPAQLCLGNNEVFEILVKNLATEMQKKPNAKYWSVSQNDNYGFCQCEK
                     CSAIDSIEGSHSGSIIRFVNKVAAMFPDKIISTLAYQYSRGATKITKPSNNVNIMFCS
                     IECDRSKPIASDKSAGSFAYDFENWSSLTTNILIWDYVVQFSNYVSPFPNFNVLQSNI
                     QFFHANGVRDIFEQGSSGNWSDFGEMKAYLISALLWNPNINVDSLCTDFANGFYGKAG
                     KALMQYHYTLEKNLDNANVMLDIYGNPVSPVNSWLSHAHMDEYKQYLNIAKEAASKNA
                     LHASRVERVSLPLWYAQLEQAKFCGTGDRGIFEKNEKGKWKVKPEIKKSVNDFVATLK
                     KQNITSLNENVLPPDKYFNDWQRIFSHGMIEHLAMEKVVTFEIPFSPKYPAKGAATLT
                     DGVGGYDDYHYNWLGWEGTDMVAVLDLRELKNVNSISCDFMEDQKSWIF"
     gene            complement(2146..>5470)
                     /locus_tag="PK736_06395"
     CDS             complement(2146..>5470)
                     /locus_tag="PK736_06395"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33056.1"
                     /translation="YSKELREMERERKRNASKKDQLENTEENEMPGYAQFKRWEWFMA
                     PRVSQTGERFDPAQVWYEMSSYYKQHPPHTFAGNWTFIGPNNTLSLSGAGRLNFVRVD
                     PINSSTIYVGSPAGGLWKSTNSGATWSTNTDNLNHVIGCTDIGIDPTNTNTMYLVTGD
                     GDAGDNYSVGLLKSTDGGNTWNPSGLSFSVSTTKQMSKILINPDNTNILMVATSAGIF
                     RSTDAAATFTKVQTGSFKDMEFKPGDPNIVYACGTEFFKSTDGGQTFAKISAGLPVAT
                     NVSRMAIGTTNNDANYLYMIVGLPAPNYGTEGFYKSTNSGTSFTKVSTPNIGTQQWYD
                     LAIASCPTNSQEVIIGGQTQFLKSTNAGVSWSAIGTTTHVDYHDLIYTSGTSIYCASD
                     GGLYHSANNGNSFTELNNNLAISQMYGFGQSTTNPNLLITGWQDNGTNLYDGNWGQTM
                     GGDGMLAFISHSNNNNMWGSQYNGSLNKSTNGGNSWQSATNGISEAGAWVTPWIEDPL
                     TANTIWAGFINVWKSTNGGTSWTKKSAFTNQGTLNAIAVSKADNNVIWAAKSAGLYVT
                     SNGGTNWTTISSLPPGTISAIECSNTDPNKAWVTFSGFTNANKVFQTNDQGATWINLS
                     SSIPNVPVNCILYVENSNDELYIGTDVGVFFKDASINIWQPFLTGLPRVIVTQLELYY
                     ATGKIRASTYGRGMWESDLYVAGSYPPGAAFGSDNKIACPGAAVHYTDYSSGQPTQWN
                     WSFPGGSPSTSTDQNPVVFYNSPGTFSASLIVTNANGVDSSSFNNFVTISTSPYNAPS
                     TVGATVCGPTVANLSAAGTGTGVLRWWDAPGGGNLLNTGSTYSPNINGSKTFYVDEDF
                     PGGSPDAVGEYSYGFGPGANFTANDIRGNYFDVLDPVVLNTVDVFSFSAGNRTIEIID
                     PQGNTLIDTTMFVPASPTIATTLNLNFTIYPGTGYFIKLRGLVDMWRNSSGAQFPYTS
                     TSINITGTNAGSSGYYYFFYNWNYTTITCNTARAACVVIDSCSSAGINNLGAIGSVDI
                     FPNPNSGIFNLNFNTQTTDNYRLKISNTIGQVVYDEKLNSFTGKFEKKIDIGSFGSGI
                     YMLSISNSQGEIVRKIEVH"
BASE COUNT         1580 a         1129 c         1011 g         1750 t
ORIGIN      
        1 aaaaatatcc agctcttttg atcttccata aaatcgcaac taatactatt cacatttttc
       61 aattccctca aatctaatac agcaaccatg tctgtgcctt cccagccaag ccagttgtaa
      121 tgataatcat cgtaacctcc tactccatcg gttaaagttg ctgcaccctt tgcaggatat
      181 tttggactaa atggaatttc gaatgtcaca actttttcca ttgccaaatg ttcaatcata
      241 ccatgcgaaa aaatcctttg ccagtcatta aaatatttat cgggaggtaa aacattttcg
      301 tttagtgagg taatgttttg ctttttcaaa gtggctacaa aatcgtttac actttttttt
      361 atttcaggct ttactttcca ttttcctttt tcattcttct cgaaaatccc tctatcaccg
      421 gtgccacaaa attttgcttg ttccagttgg gcataccaca aaggcaatga aacacgttca
      481 acgcgggagg catgcaatgc atttttactt gctgcttctt ttgcaatatt caaatactgt
      541 ttatactcat ccatgtgcgc atgcgaaagc catgagttta caggcgatac aggattgcca
      601 taaatgtcga gcattacatt tgcattgtct aaattttttt ccaaagtata atgatattgc
      661 atcaatgcct tgcctgcttt accataaaat ccatttgcaa aatcggtgca gagtgaatca
      721 acatttatat tgggattcca taacaatgcg gaaatcaaat atgctttcat ttcaccaaag
      781 tcgctccaat tgcccgaact gccttgttcg aaaatatcgc gcactccgtt tgcatgaaag
      841 aactgaatgt tggattgcag cacattgaaa tttggaaaag gtgaaacata atttgaaaat
      901 tgcactacat aatcccatat aagaatattg gttgtgaggc tactccaatt ttcaaaatca
      961 taagcaaaag accctgccga tttatcggag gcgataggtt tgcttctatc gcattctata
     1021 ctgcaaaaca taatatttac attgtttgaa ggctttgtaa ttttggttgc accgcgcgaa
     1081 tactgataag caagagtcga aataatttta tcgggaaaca tggctgctac tttatttaca
     1141 aaacgaatta tcgaacccga atgactgcct tcaattgaat cgatagcact gcatttttcg
     1201 cattggcaaa atccataatt atcattttgg ctcaccgacc aatatttggc attcggtttt
     1261 ttttgcattt cggtggcaag atttttaaca agtatttcaa acacttcatt gttgcccaaa
     1321 cacaattgtg ccggctgacg ttttccgcca taaaatgaaa agtactcggg gtgcgaatcg
     1381 aaatattttt cttcgggtac caatttatta aatgtgtgca cccacatgcc ccattgcgaa
     1441 ctactatcgt ttggaaaatg gcgcataagt ttgtgccact cgcggtactc atcgtcaaca
     1501 gttgctgtgt aataatattc gcgaaaatcc attgcaggaa tttctacatc atcaattata
     1561 tcaggaataa cgagtgttgc gtttttggga acaacagctt catcgttact gtattttcgg
     1621 cagccacaat atttttcgag aaaagtataa actgcatata ctactccctt gcctgtgcct
     1681 ccatttaaga tgaggatgtt tttttgtgtg ctaatactaa agccatcagg atgatttatt
     1741 ttattttctt ttaaatcatt gcgagaagta ttgccaacaa ttatttcgaa atcggatttg
     1801 gtatgtgtat catccacaat caaaaaactt gctccggtaa ttttaaacaa atatttgttc
     1861 agcacatctg ccgcatgttt ttcattttca tttgctttgg cacttatgac aatcgtatac
     1921 tttgcttttc catcagtaga aagtgtgagc gaaaaacatt ttgaaaaaca aattataaaa
     1981 aatattgtga gtgtaaactg atgatgtatt ctatgtttca tgctgttggc ttttaaaaag
     2041 ataatggacg aaagtattta ttaaataaaa aacccgcaag atttaaaaaa tcttgcgggt
     2101 ttgaagaacg ttatatcaac aatgtttaaa gaagcgaatt aaaaattaat ggacctctat
     2161 cttccttact atttcgcctt gcgaatttga tattgacaac atataaattc ccgagccaaa
     2221 acttccgata tcaattttct tttcgaattt tcctgtgaaa gaatttagtt tttcatcata
     2281 tacaacttgt cctatagtgt tcgaaatttt caatcgataa ttatctgttg tttgtgtgtt
     2341 gaaattcaaa ttaaatattc cactgttggg gtttggaaaa atatctacac tgccgattgc
     2401 acctaaatta tttatgccgg cagaactaca actatcaata actacacaag ctgcacgtgc
     2461 tgtgttgcag gtaatagtgg tataattcca gttataaaaa aagtaatagt aacccgatga
     2521 tcctgcattg gtaccggtta tatttattga agtacttgtg taaggaaatt gcgcaccact
     2581 actgttacgc cacatatcga ccaggccacg taacttaatg aaatagcctg ttccgggata
     2641 gatggtaaaa ttaagattga gcgtagttgc tatggtagga ctggccggaa caaacatggt
     2701 tgtatcaatc aatgtattgc cctggggatc gattatttca atagtacgat taccggcact
     2761 gaaagaaaaa acatcaacag tattgagcac taccggatcg agtacgtcaa aataattgcc
     2821 gcgaatatca ttggccgtaa aatttgcccc cggaccaaaa ccataactat actctcctac
     2881 tgcatcgggc gagccaccgg gaaaatcttc gtcaacataa aaagttttgc tgccatttat
     2941 gtttggagaa tacgttgaac cggtatttaa taaattgcca ccaccgggtg catcccacca
     3001 acgtaacaca cctgtgcctg tgcccgctgc cgaaagattt gcaactgtgg gtccacaaac
     3061 ggtagcacct actgtggatg gcgcattata aggacttgta gaaattgtta caaaattatt
     3121 gaacgagctt gagtctactc catttgcatt ggtaactata agcgaggccg agaaagtgcc
     3181 cggactatta taaaacacca cagggttttg atcggtagaa gttgaaggcg aaccaccggg
     3241 aaacgaccag ttccattgtg taggttgtcc cgaagaataa tcggtgtaat gtactgcggc
     3301 accgggacat gcgattttat tatcggatcc aaatgctgca cccggaggat aactaccagc
     3361 aacgtataaa tccgactccc acataccacg gccataagtt gatgcacgta tttttcctgt
     3421 agcataatat aattcaagtt gtgttacaat aacgcgtggc aatcctgtga ggaatggctg
     3481 ccagatattt attgacgcat ctttaaaaaa cacacccaca tcggttccaa tgtataattc
     3541 atcgttcgaa ttttcgacat acaaaataca gttgaccgga acgttaggaa tcgagctcga
     3601 aagatttatc catgttgccc cctgatcgtt tgtttgaaaa actttattgg catttgtaaa
     3661 tcctgaaaaa gtaacccatg ctttgttagg atcggtgttg ctacattcta ttgcacttat
     3721 agttccgggt ggcaaacttg aaatggttgt ccaatttgtt ccgccatttg atgtaacata
     3781 taatcctgca ctttttgctg cccaaatcac attgttatcg gctttcgaaa ctgctattgc
     3841 atttaaagta ccttgattgg taaaagcact tttctttgtc cacgatgttc cgccatttgt
     3901 acttttccaa acatttataa aacctgccca tattgtattt gcagttaaag gatcttctat
     3961 ccatggcgta acccatgcac cggcttcgct aattccattt gtagccgact gccagctatt
     4021 gccaccattt gttgacttgt ttaatgagcc attgtattgc gatccccaca tattattatt
     4081 gttgctgtgg ctaataaaag caagcatgcc atcacctccc attgtttgtc cccaattgcc
     4141 atcgtataaa ttagtgccgt tatcctgcca tcctgttatc aaaagatttg gattggtagt
     4201 agattgccca aagccataca tctgagaaat agcgagatta ttattcaatt cggtaaaact
     4261 atttccattg tttgccgaat gatataaacc tccatcgctt gcgcaatata ttgatgtgcc
     4321 acttgtgtaa ataaggtcgt gataatcaac atgagttgta gttccaattg cactccatga
     4381 tacgccagcg tttgtagatt ttaaaaattg agtttgtcct ccaatgataa cttcctgact
     4441 gtttgttggg cacgatgcaa tggccaaatc ataccattgt tgtgtaccaa tatttggtgt
     4501 gcttactttt gtaaaacttg tgccgctgtt tgtcgattta taaaaacctt cggtgccata
     4561 atttggtgct ggtaaaccaa caatcatata taaatagttt gcatcgttgt tagtggtgcc
     4621 aattgccatg cggcttacat ttgttgctac aggaaggcct gccgatattt ttgcaaatgt
     4681 ttgtccgcca tctgtcgatt taaaaaattc tgtaccacat gcataaacaa tattgggatc
     4741 accgggctta aattccatat ctttaaacga acctgtttgc acttttgtaa atgttgctgc
     4801 tgcatcggtg ctgcgaaaaa ttcccgcaga tgttgcaacc ataagtatgt ttgtattatc
     4861 agggttaata agaatcttgc tcatttgttt tgttgtcgaa accgaaaacg ataaccctga
     4921 tggattccat gtattgccgc catcggtcga tttaagcaag cctacagaat aattatcgcc
     4981 cgcatcgcca tcaccggtaa caaggtacat tgtgtttgtg tttgtagggt ctatgcctat
     5041 atcggtgcag cctattacat gattcaggtt gtcggtattg gtagaccacg tggccccact
     5101 atttgtcgac ttccataatc ctccggcagg ggaacctacg tatatagtac ttgaattaat
     5161 tggatctact cgcacaaaat ttaaacggcc cgctcccgac aatgacaatg tattatttgg
     5221 accaatgaaa gtccagttac cggcaaatgt gtgaggcgga tgctgcttgt agtaactact
     5281 catttcgtac cacacttgag caggatcgaa acgctcaccg gtttgtgata cacgtggtgc
     5341 cataaaccat tcccaacgct taaactgcgc atagcccggc atttcgtttt cttcggtatt
     5401 ttcaagttga tcttttttcg atgcattgcg tttgcgttct ctttccattt cgcgcaactc
     5461 tttcgaatac
//
LOCUS       DAORQA010000111         5466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3781_length_5466_cov_13.953613, whole genome shotgun sequence.
ACCESSION   DAORQA010000111 DAORQA010000000
VERSION     DAORQA010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3781_length_5466_cov_13.953613"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PK736_06400"
     CDS             <1..303
                     /locus_tag="PK736_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRC33057.1"
                     /translation="QRYKELQDIIAILGMDELSDEDKLVVHRARRVQRFLSQPFFVAE
                     QFTGLKGVLVPIEDTIKGFNMIMNGEVDEYPEAAFNLVGNIDDAIAKGKKLLAEAN"
     gene            519..764
                     /gene="atpC"
                     /locus_tag="PK736_06405"
     CDS             519..764
                     /gene="atpC"
                     /locus_tag="PK736_06405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRC33058.1"
                     /translation="MQIDIITPDKKIYSGECTSVSVPGSDGRMQFLKNHAPLISSLGN
                     GKVKVKATDGMHEFNISGGVVEVLKNKIILLAEKVVV"
     gene            complement(1011..1427)
                     /locus_tag="PK736_06410"
     CDS             complement(1011..1427)
                     /locus_tag="PK736_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33059.1"
                     /translation="MRAIFCILLFIICTTSGCRKSRNQIPNTPIDLYIDTKLPSYISL
                     NIVGGWIYASSGYKGLIIYRRSQDAFNAYERACPYDPTDDCSLVEVLKDNITAVDSCC
                     GSQYLITDGNVIKGPATQTLQQYQTTFDGTILHVFN"
     gene            1534..1821
                     /locus_tag="PK736_06415"
     CDS             1534..1821
                     /locus_tag="PK736_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33060.1"
                     /translation="MSKKIVGFVLLIMMLSSCVTSADAQSRSAQRKIDKVEKKKAEKK
                     KIEERTFKKFQKRQLKIQTKETRKRMKKNRRTKSSPYGRNNKSTWWYRLWH"
     gene            1797..2474
                     /locus_tag="PK736_06420"
     CDS             1797..2474
                     /locus_tag="PK736_06420"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRC33061.1"
                     /translation="MVVSPLALIIMKKYFPLRVSTLSINWLNIICALVFIIPAKVCQS
                     QEINDSLLIATARVYRNLSEALQNKDSVYILDLSKQKFATIPPEVFLLTNLRILKMEK
                     CKLQSINSEIEKLTLLHELNLANNKIVAITPKIGTLNNLKVLNLNRNLIEEIPSEIGN
                     LRNLRELYMWDNELSIFPNELKQLSALKIFELRGILIDETDQQRIKALLPDCKVMMSP
                     SCNCKTY"
     gene            complement(2599..2889)
                     /locus_tag="PK736_06425"
     CDS             complement(2599..2889)
                     /locus_tag="PK736_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC33062.1"
                     /translation="METKSWKKIKDSVYGIKGTTRRDELDRDFESFKIGLLLRKARED
                     KNLTQNQLADLVDKKRTFISRIENNGGNLTLKTLFEIVEKGLGGKVKISIEL"
     gene            complement(2891..3236)
                     /locus_tag="PK736_06430"
                     /pseudo
     CDS             complement(2891..3236)
                     /locus_tag="PK736_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552027.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3343..3516
                     /locus_tag="PK736_06435"
     CDS             3343..3516
                     /locus_tag="PK736_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33063.1"
                     /translation="MWDNELSIFPDEIKKLSALKIFELRGILIDETDQQRIKSLLPDC
                     KIMMSPSCNCKTY"
     gene            complement(3523..3876)
                     /locus_tag="PK736_06440"
     CDS             complement(3523..3876)
                     /locus_tag="PK736_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33064.1"
                     /translation="METKLNIETRVIENAHIFLWLIKDMCWVSEYKTIGMIMVFPTIA
                     TAIYITWLNRNMATELVHNLAVCCWISANSIWMIGEFFYNDTTRSFAKIFFIAGLSIL
                     ILYYVFHFLKSKISV"
     gene            3983..4828
                     /locus_tag="PK736_06445"
     CDS             3983..4828
                     /locus_tag="PK736_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33065.1"
                     /translation="MKKYTLFFLAMLLMNVAFSGGPWSLGKGGLYFQAGGSTINYNSI
                     FNKNGDVVPLQIYDKVSDNTFQLYSEIGLSKKWTLKLNVPYKYIINSISSTNDNISGL
                     SNVSATIQYNIEGKKWPTALGFALFAPAQKEKTTRSTLTEYNLAGYDAFTFNPYFSIG
                     TSKGKWYTFANIGAGIMTNNYSSYSKVDAEFGIKVLKYFWLAAIIDTRIPIQNNSPDV
                     ASIGTINLLNKLYVNNQQYVSYGAKVAVPFTSKFGFNAGMNLAALSRNAAAAPAFSAS
                     IYYKR"
     gene            5072..5320
                     /locus_tag="PK736_06450"
     CDS             5072..5320
                     /locus_tag="PK736_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33066.1"
                     /translation="MELITLKQKLHNYLEVANEKKIKAIYTMLETDIEESLVEYTLDL
                     KADLDKRKKEYKAGNLKMITATESKARIDKLRNQKSKM"
BASE COUNT         1887 a          899 c          914 g         1766 t
ORIGIN      
        1 caacgttaca aagaacttca agatattatt gccatccttg gtatggatga attgagtgac
       61 gaagataaat tggtggttca ccgtgcccgc agggtacaac gtttcttatc gcaaccattt
      121 tttgttgccg aacagtttac aggattgaaa ggcgttttgg ttccaattga agataccatc
      181 aaaggattta atatgattat gaatggcgaa gtggatgagt atccagaagc ggccttcaac
      241 cttgtaggta acattgatga tgctatagct aaaggtaaaa aattattggc agaggcgaat
      301 taagttttta gtttggggga agtttttagt ttttagttct tagtttttag ttggctaacg
      361 caaaaaaaac tatttgctgg tttataaatt gaaaactgta tttcaaacta aaaactataa
      421 actgaatttt aaactgaaaa ctataaactg aatttcaaac tgaaaactga gtttcaaact
      481 taaaactgta tttcaaactt aaaaccgaat taaaaaaaat gcaaatagac atcatcacac
      541 cggataaaaa aatatatagt ggcgagtgca catcggttag tgtgcccggt tctgacggac
      601 gcatgcaatt tttgaaaaac catgctccgc tgataagttc acttggtaat ggaaaagtaa
      661 aagtgaaagc taccgatggt atgcacgaat ttaatattag tggtggcgta gttgaggtat
      721 taaagaataa aataattctc cttgccgaaa aggtggttgt ataaaactgt ttttcattta
      781 ttaaaaataa taagagcacc cttcatttgg agggtgtttt ttttgaaata gtttttagat
      841 aataataagc aaaagctttc tatatgaaaa tttgaatgct gatttccatg atttttgtca
      901 tttcaacttg cgattttact aaagataaat ctgcgttcat cctgaaaatc tgtgttcgtt
      961 aaaaagggtt ctttaagaga ctaacttgca atttttgttt gcaagaaaaa tcaattaaaa
     1021 acatgtaaaa tggtgccatc gaacgtggtt tggtattgtt gtaatgtctg tgttgccggg
     1081 ccttttatca cattgccatc ggtaattaaa tattgcgagc cacagcatga atccactgcg
     1141 gtaatgttat ctttaagcac ttcaacaagg ctgcaatcgt ccgtggggtc gtaagggcat
     1201 gcacgttcat aagcattaaa tgcatcctgg cttcgcctgt aaattatcaa ccctttataa
     1261 cctgacgagg cgtaaatcca accaccaaca atgttcaacg aaatgtatga aggcaatttg
     1321 gtatctatat ataaatcaat tggcgtgttg ggaatttgat ttcgcgactt tcggcaaccg
     1381 ctggttgtgc atatgataaa taataaaatg caaaaaattg ctcgcatatt ttggtataaa
     1441 ataataatca aagatggaaa ttattttcca tagttgttgt gtgctaaaaa taattagaat
     1501 gatatttgcg ttcttcactt caattaactt aggatgtcca aaaaaattgt tggtttcgtt
     1561 ttattaataa tgatgctctc atcttgtgta acaagcgctg atgcacaaag ccgcagtgcg
     1621 caacgcaaga ttgataaagt ggaaaagaaa aaagcggaga agaaaaaaat tgaagaacgg
     1681 acattcaaaa aatttcaaaa gcggcagctt aaaatacaaa ccaaagaaac acgaaagcga
     1741 atgaaaaaaa acagacgcac caaaagttct ccatatggac gcaacaataa aagtacatgg
     1801 tggtatcgcc tttggcattg attatcatga aaaaatattt ccctttgcga gtatctacat
     1861 tatccattaa ttggttaaac attatttgtg cattggtttt tataatacct gccaaggttt
     1921 gtcaatctca agaaataaat gattcgctat taatagccac tgccagggtg tatcgcaatc
     1981 ttagcgaagc attacaaaat aaagacagcg tttatattct cgatttgtcg aaacaaaaat
     2041 ttgcgactat tccacctgag gtttttttat tgacgaatct tcgcattttg aaaatggaaa
     2101 aatgcaaact tcaaagcatc aattccgaaa tagaaaaatt gaccttgttg cacgaattaa
     2161 atttagcaaa taataaaatt gtagctatta ctccaaagat agggacattg aataacctaa
     2221 aggtgctcaa tcttaataga aatttgattg aagaaattcc ttccgaaatt ggcaatctcc
     2281 gcaacctccg cgagctttat atgtgggata acgaactgag tatattccct aacgaactaa
     2341 aacaacttag tgccctaaaa atatttgaac tgcgcggcat tttaattgat gaaaccgacc
     2401 agcaacgcat aaaagcatta ttgcccgatt gcaaagtaat gatgtcacca tcgtgcaatt
     2461 gcaaaactta ctgataattg atttagtatt tatgtttgtt tataatatca tgaatagaca
     2521 aatgcataat gagagtgcag caaaaaataa aattgcatgc tattcttttt tattctttta
     2581 gtttccaatt taaaaaaact acaactctat cgaaattttc actttccctc caagtccttt
     2641 ttcaacgatt tcgaaaaggg tttttaaagt taaatttccg ccattgtttt ctattcgcga
     2701 aatgaaagtt cttttcttat caactagatc tgcgagttga ttttgtgtta aatttttatc
     2761 ctctcgggct ttgcgtaaaa gcaaaccaat tttaaaactt tcaaaatctc tgtcaagttc
     2821 atcacgtctg gttgttccct ttattccgta aacggaatct tttatttttt tccaactctt
     2881 agtctccatt ttatttattt tttgcttcat aatattcttt catcaaacct aatgcttttt
     2941 ctatttcttt aggtggcgtt ttttgtgttt ttttttacaa atccattcaa aagcactacg
     3001 agtttgccct tatcaaaaaa gcaaaaaact cgccatatat ctgaacctaa ttgtatccta
     3061 gcttcataaa ggccattcgt tcctactatt gctttcaaat agtttgatgg aattctttca
     3121 agagtttcaa ttgcttctat tattttgaat attttatctt gaactttttt agtttgtaag
     3181 tttaagaaat cttcaaaata atttttgtag gctattactt ctcttatttt ttccattcgc
     3241 acaaatgtaa cttataagtt accacactcc aaattatcga ataatttttt tcgaatatgt
     3301 atttttcgtg caattgaaat aggcaacctc agcgaactgt atatgtggga caatgagcta
     3361 agtatttttc ccgatgaaat aaaaaaactc agtgctctaa aaatatttga attgcgcgga
     3421 attttaattg atgaaaccga ccagcaacgc ataaaatctt tgttgcccga ttgcaaaata
     3481 atgatgtcgc cttcgtgcaa ttgcaaaact tattgacttt gtttaaaccg aaattttgct
     3541 ttttaaaaag tgaaatacat aatataaaat aagaatgctt aatccggcaa taaaaaatat
     3601 ttttgcaaaa gaacgagtgg tatcgttgta aaaaaactct cctatcatcc agattgaatt
     3661 ggcacttatc caacagcata ctgccagatt atgaaccaat tcggtggcca tatttctatt
     3721 aagccaagta atataaattg ctgtagctat tgtgggaaaa accattatca tacctatcgt
     3781 tttatattcc gacacccaac acatatcttt tataagccac aaaaaaatgt gtgcgttttc
     3841 tattaccctt gtttcaatat ttaatttggt ttccattttg caaaattttg aatgctgaaa
     3901 atacgtcatt ttcaagacat aaggagaaat taattttact taattttacc gaaattgttt
     3961 tcggaaaaat aaaatccaaa ttatgaaaaa atacactttg ttctttttgg caatgttgtt
     4021 aatgaatgtt gcatttagcg gtggcccctg gtcgttgggc aaaggcggtt tgtactttca
     4081 ggcaggaggc tcaacaatta attacaatag tatatttaat aaaaatggag atgtagtgcc
     4141 attacaaatt tacgataaag taagcgacaa tacttttcaa ctttattccg aaattgggtt
     4201 atcaaaaaaa tggacgctca aacttaatgt tccttacaag tatatcataa attctatttc
     4261 atctacaaat gataacataa gcggattgag taatgtatcg gcaactattc aatataatat
     4321 tgagggaaag aaatggccca ccgctttagg ctttgctctt tttgcgcctg cgcaaaaaga
     4381 aaaaacaaca cgttcaactc taaccgaata taatcttgca ggatatgatg catttacttt
     4441 taatccatac ttttccattg gcaccagtaa aggaaagtgg tatacgtttg caaatattgg
     4501 tgcaggaatt atgactaata attacagttc atactcgaag gttgatgccg agtttggtat
     4561 taaagtacta aaatattttt ggctggcggc aattatcgac acacgaattc caattcaaaa
     4621 taatagccct gatgtagcct ccattggcac aattaatctg ctgaacaaac tatacgtaaa
     4681 caaccaacaa tatgtttcgt acggtgcaaa agtggctgtg cctttcacct caaaatttgg
     4741 tttcaatgca ggaatgaatc ttgcagcatt atcgcgtaat gccgcagcgg cacccgcttt
     4801 ttcggctagt atttactata agcggtaaat tgggattggc taaaaaataa ccttggccat
     4861 tctttgataa acgtattaac taagcaataa tcttaagcta cctcttgtat aataattcgc
     4921 ctattaaact aaaaataaaa catctattga agtttataaa agcaagatat tttacattta
     4981 gaataaaaaa gattactgcg atgctaaatt agaattgaaa taatgtgcgt taatttattt
     5041 gtaactttgt aaagtaattt aaaatcaaga aatggaatta ataacactaa agcaaaagct
     5101 gcataattat ttggaagtag ccaacgaaaa aaaaattaaa gcaatctaca ccatgcttga
     5161 aacagatatt gaagaatctt tggtagaata tactttagat ttgaaagcag atttagataa
     5221 aagaaaaaag gaatataaag cagggaattt aaaaatgata actgcaactg aaagcaaagc
     5281 ccgaattgac aaattgcgaa accagaaaag taaaatgtaa tgttccaata tgtgatacac
     5341 cctgacgctc aacttgagtt agaaaacgcg tttatctggt atgcctcaag aagcgaaaga
     5401 gtagcacata attttgtaaa tcaagtgcat caaaccattt ttactatttg ctcaaatccc
     5461 aatcaa
//
LOCUS       DAORQA010000112         5451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3796_length_5451_cov_8.093217, whole genome shotgun sequence.
ACCESSION   DAORQA010000112 DAORQA010000000
VERSION     DAORQA010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3796_length_5451_cov_8.093217"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..712)
                     /locus_tag="PK736_06455"
     CDS             complement(188..712)
                     /locus_tag="PK736_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33067.1"
                     /translation="MKKINLIFILFVSAFTSNAQTIQRSVIGSAGGTTTAGSITLSST
                     VGETFTNKLTTANGSIKQGFQQGNILIARIANTELPATISEAKTIAPEKVETELMLNI
                     FPNPTTDFVNIATETDEQINLTVIDAQGKIIKQLQHSSTQTQIDFTQLTAGTYFIVAQ
                     SANSRNTYKVIKQQ"
     gene            complement(733..2301)
                     /locus_tag="PK736_06460"
     CDS             complement(733..2301)
                     /locus_tag="PK736_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33068.1"
                     /translation="MKLKITFIIAALFVSLTSLMAQSPNMINYQGVAHNVNGTAIANQ
                     NIKVRLKIRQGTAQGTVKYSEVRSLTTDASGLFTIQIGSAGAVSTTGSWASITWETGA
                     KFLQVEMDAAGGSNYTDMGTQQMLSVPYAQYSNMAGALSPSAKIDLTQINGSGATNGQ
                     ILKFNGTNWIPAAASNSPFTLPYIATDANATSIGITNSNTLGGVALYGKTTSTNANST
                     GVRGETTVAGSTGVNGKANNATAVGIKGENALGTAIEGITNAASYPAVEGWSTAANGG
                     KGVVGIANNGTSAIGVEGNANAGIGVSGYSNTYRGVSGGSISGTALYGYSTSGYGLEV
                     NGKIKISGGNTTPGAGKVLTSDASGNATWQAPSATQPKVAFKAAKATASIPHNQNTNI
                     NWTSELYDLSNNFDIVTKEFTVPVSGVYHFDASFYFHLSSLVYNLSEANITIQSNRNG
                     TISYLSSHDGVIKINTSYSQAISATSVDARLLAGDKVYVQVIQFNGGSLTATQAAATS
                     EAVHYFSGHLVIAD"
     assembly_gap    2503..2570
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2580..3197
                     /gene="udk"
                     /locus_tag="PK736_06465"
     CDS             2580..3197
                     /gene="udk"
                     /locus_tag="PK736_06465"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008021729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HRC33069.1"
                     /translation="MLIIGIAGGSGSGKTTVVKEVMSSFSLNQVAILSQDSYYKDSSH
                     ITIEDRKSINFDHPESIEFDLLVNHMHQLRKGVKIEQPLYDYITSTRLKEVNNIEPNN
                     VIIVEGILLFTDKRVRELCDIKVFVDAEPDDRLIRIIERDMRERGRSAHQVINRYTVV
                     KEMHTQFIEPTKRFADVIIPQGGENKVAINMLVATIRQKLLKSNQ"
     gene            3361..5031
                     /locus_tag="PK736_06470"
     CDS             3361..5031
                     /locus_tag="PK736_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HRC33070.1"
                     /translation="MMRILLTTLNSKYIHTNLAIRLLYQLNRHHAGLQWKEYTIKMAQ
                     DDVAQECAQFDIVCFSCYIWNITQTLAVAEKIKAKNPFAKILLGGPEVSYEWKEIIAL
                     PYIDYIITGEGEIPFAEFLNNFPAVENIGNVVSKKDGNVLYFPQAVEFDLKNYGDIIP
                     YAYDNKEELATKVLYIETSRGCPYKCEFCLASLDNKVRYLPNESIKQNLLFMMEHGKV
                     IKFLDRTFNVKKDFTIDIFSFILKHHNPRNIFQFEITADIVHQDIIKYIDDHVPPGLF
                     RFEIGIQTVNQQANLQVSRKQNFDKTKGIIQQLQTKIEMHLDLIVGLPLDYWNDIKYS
                     IEEVFKLFAPELQLGFLKFLKGTPVRHKAAQHDFVYDPLPPYQIIESKYLSREQLSQI
                     VILENALEIYWNKNRTQHTLKYVTQQYSIFDFLLGLGNYFLSRRNFHEYQLTEVYHII
                     FDFIKINYADDVLLPQLVAIDYYLNSKVKPKILFCNELERTARQNIIDTHQLNHHKYR
                     FVIFPITFDYHLLKSENMIRHSKQNIIIQYDGKNYATIVEVESAGILI"
BASE COUNT         1644 a          984 c         1001 g         1754 t
ORIGIN      
        1 gtaatacgaa gtgtggttca tttgaaatga ggtgtgtttt ttttggaacg gagtgttttt
       61 ttgggggagg ctgttagctt ttggctgcga gcttttagtc tttggctttg agctttttgt
      121 tgattttaat aaaaacaaaa aaaccggact gttagcccgg catttttaac tttaaaatta
      181 tttttttcta ctgttgcttt atcactttgt acgtgtttcg cgagttagca ctttgtgcaa
      241 caataaaata tgtgcctgct gttaactgtg taaaatctat ttgcgtttgg gttgaactat
      301 gttgcaattg cttaattatt tttccttgcg catctatcac cgttaaattt atttgctcat
      361 cggtttctgt tgcaatgttt acaaaatctg ttgtcgggtt cggaaaaata tttagcatca
      421 attctgtttc aactttttct ggtgctattg tttttgcttc cgaaatggtt gcaggcaatt
      481 ctgtatttgc aatgcgcgca attaaaatat ttccttgttg aaacccttgc ttaatgctgc
      541 cgtttgctgt ggtgagtttg tttgtaaatg tctcgccaac tgttgatgat aaggtaatgc
      601 ttcccgctgt tgtcgtgcct cctgccgaac cgattacact gcgctgtatt gtttgagcat
      661 ttgatgtaaa tgcgcttaca aatagaatga atattaaatt tatttttttc attttgatta
      721 ttttttaagt gattagtctg cgataaccaa atgaccagaa aaataatgaa cggcttcgct
      781 tgttgctgca gcttgagtag ctgttaatga accaccgtta aactgaataa cttgaacgta
      841 taccttatca cccgccagca atcgcgcgtc aacacttgtt gcgcttatgg cttgactata
      901 tgaagtgttg atttttataa caccatcatg tgaggataaa taagatattg taccattacg
      961 atttgattgt atagtaatat ttgcctctga taaattatac acaagcgatg acaaatgaaa
     1021 ataaaacgaa gcatcaaaat ggtaaacacc tgaaaccgga acagtaaatt cttttgttac
     1081 aatatcaaag ttattgctca aatcatataa ctcacttgtc cagttaatat tcgtattttg
     1141 attatgaggt atgcttgcgg tagcctttgc agctttaaat gcaacctttg gctgtgttgc
     1201 cgatggtgct tgccaggttg catttccgct tgcatcgctt gtaagtactt tgcctgcacc
     1261 gggggtagta ttacctccac ttatttttat cttaccgtta acttctaaac cataacccga
     1321 agtgctatat ccatacaatg cagttccaga aatagaacct ccgctaactc cacggtaagt
     1381 attgctataa ccactaactc caattccagc gttggcattg ccttctaccc caattgcaga
     1441 agtaccatta tttgcaatac ctactacacc ttttcctcca ttcgctgctg tactccatcc
     1501 ttccactgca gggtaagatg cagcatttgt aataccttct atagctgttc ccaaagcatt
     1561 ctcgcctttt atgccaactg ctgttgcatt gtttgcttta ccatttacgc ccgtgctgcc
     1621 ggcaacggta gtttcgccac gtacaccagt cgagtttgca tttgtactgg tcgttttacc
     1681 atataatgca actccgccta aggtgttgct gtttgtaatt cctatggatg tagcattggc
     1741 gtcagttgca atgtaaggca atgtaaatgg cgaattggaa gctgctgcag gtatccaatt
     1801 tgttccgttg aattttaaaa tctgtccgtt tgtagcaccc gaaccattga tttgagttaa
     1861 gtctatttta gctgatgggc taagagcacc tgccatatta ctatactgtg cataaggtac
     1921 gcttagcatt tgttgtgtgc ccatgtctgt ataattgctt ccacctgccg catccatttc
     1981 aacttgtaaa aactttgctc ctgtttccca tgtaattgat gcccatgaac ctgttgtact
     2041 tacagcaccg gcacttccaa tttgaatagt gaataagccc gaggcatcgg ttgttaagct
     2101 gcgcacctca ctatatttta cagtgccttg tgctgtgcct tgacggattt ttaaacgcac
     2161 cttaatgttt tgatttgcaa tagctgttcc gtttacattg tgtgctacac cttgatagtt
     2221 tatcatgttt ggcgattgtg ccattaatga tgtcaggctc acaaagagcg ctgcgatgat
     2281 gaatgtgatt ttcaatttca ttttttgatt ttttgatttt ttttgattaa taattatggt
     2341 gcaaacaaac cacacagtgc aagcgattaa aaattttgta atgcgaagtg tggtggattt
     2401 gaaatgaggt gtgtgtttta ggaaatgagc cgtttgctat tggcttttag ctgctggctg
     2461 ttggcctttt gctgtttgat aaaaacaatt cgaaattctt ttnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaataatca
     2581 tgctcataat tggaattgca ggaggttccg gctcgggaaa aacaacggtt gtaaaagaag
     2641 tgatgagttc gttttcgctc aatcaggttg ctatactatc gcaggatagt tattacaaag
     2701 acagtagcca catcactatc gaagacagga agagcatcaa ttttgaccac cccgaaagta
     2761 tagagtttga cttgcttgtg aatcatatgc atcaacttcg caaaggtgta aaaatcgaac
     2821 aaccacttta cgattacatt acatcaaccc gcctgaaaga agtaaacaat atagagccaa
     2881 acaatgtaat aatagtagaa ggcattttat tatttaccga taaacgtgtg cgcgaactgt
     2941 gcgatataaa agtatttgta gatgccgaac ccgatgaccg acttataaga attattgagc
     3001 gcgatatgcg cgagcgcgga cgcagtgccc atcaggtgat aaaccgatac actgttgtga
     3061 aagaaatgca cacgcaattt attgaaccta caaaacgatt tgccgatgtt ataattccgc
     3121 agggaggcga aaataaagtg gcgataaata tgctggttgc aacaattaga cagaagctgc
     3181 taaagtctaa tcaatagttt tcttttagtt tcagagtcct cattcgtttt tgtctctaag
     3241 aaaattaaga gtctccaaca tctttaaaaa aaataataga tagttgggac tctgaatttg
     3301 cggagaaact aaaattagcc ccacattttt atctcactat atttgccatc aataatattt
     3361 atgatgcgca ttttactcac tacacttaat tcgaagtaca tacacaccaa tcttgctata
     3421 cggttgttgt atcaattgaa ccggcatcat gcagggttgc agtggaaaga gtacactata
     3481 aaaatggcac aagatgatgt tgcccaagag tgcgcgcaat tcgacattgt atgctttagt
     3541 tgttatatat ggaatataac acaaacgctt gccgtagccg aaaaaataaa agccaaaaac
     3601 ccatttgcaa aaatccttct tggtggtccc gaagtgagtt acgaatggaa agaaattatt
     3661 gcacttcctt atatcgatta cattattacc ggtgaaggcg aaattccttt tgccgaattt
     3721 ctaaataatt ttcctgctgt agaaaatatt gggaatgtag tgagcaaaaa agatggtaat
     3781 gttttatatt tcccacaagc ggtagaattt gatttgaaaa attatggcga tattattcct
     3841 tatgcttatg ataacaagga ggagttggcc accaaagtgc tgtatataga aacatctcgt
     3901 ggttgccctt ataaatgcga attttgtttg gcaagtttag ataacaaagt tcgctactta
     3961 cccaacgaaa gcataaagca aaatttattg ttcatgatgg aacatggtaa ggtgataaaa
     4021 tttttggacc gcacctttaa cgtaaaaaaa gattttacca tcgatatatt ttcattcatt
     4081 ttaaaacatc ataatccaag aaatattttt cagttcgaaa tcacggccga tattgtacat
     4141 caggacataa taaaatatat tgatgatcat gtaccgccag gattgtttcg tttcgagatt
     4201 ggtatacaaa cagtaaacca acaagcgaat ttacaggtaa gccgcaagca aaatttcgat
     4261 aaaactaaag gcatcataca acaattgcaa accaaaatag aaatgcatct cgatttgatt
     4321 gttggtctgc ctttagatta ttggaacgat ataaaataca gtatcgaaga agtttttaaa
     4381 ttattcgccc cggaattaca attgggtttt ctcaaatttt tgaaaggtac acccgtgcgg
     4441 cacaaggctg cgcaacacga ttttgtatac gatcccttgc caccttatca aattatagaa
     4501 agcaaatatt tgtcgcgcga gcaattgagt caaattgtaa ttttagaaaa cgctctcgaa
     4561 atttattgga acaaaaatcg cacgcaacac acattaaaat atgtaacgca acaatacagc
     4621 attttcgatt ttttactcgg tttgggaaat tattttcttt cgcgcagaaa ttttcacgag
     4681 tatcaattga cagaagtgta tcatataatt ttcgatttta taaaaattaa ttatgctgac
     4741 gatgtgttac tgccacaact tgttgccatc gattattatt taaacagtaa agtgaaacca
     4801 aagattttat tttgtaatga acttgaaaga acagcaaggc aaaatattat cgatacccat
     4861 caactcaatc accacaaata tcgttttgta atttttccaa tcactttcga ttatcacttg
     4921 ttgaaatccg aaaatatgat tcgccactcg aaacaaaata tcattataca atatgatgga
     4981 aaaaactatg caacaattgt agaggtggag agtgcaggca ttctaattta aaaattattt
     5041 atactttgaa tggaataagt ttcaccactg ttaagttgaa atattagaaa atcaacatcg
     5101 aactctgaaa gggttcaatt tgaataacca ctggtcaata caggtggata agagcatata
     5161 taaacgaaca gaaccagaaa gggttcaacg tggtgagcat caaaccatat ttgaaacact
     5221 aagccaaatt gaaaatgtgg aaatttatat catttgcggc atggttactt ttgttgcctt
     5281 taaaagctac tataagaata taaatgaaaa aaatacgctc ccgcagattt tcgcagattc
     5341 aattccgcag gttttttaat acaagcaact atctttatct acgcatatct taggaacaaa
     5401 ttttaggttt gcatttttac cattgaccaa aaatattaca tttgcaaaaa c
//
LOCUS       DAORQA010000113         5441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3807_length_5441_cov_10.268102, whole genome shotgun sequence.
ACCESSION   DAORQA010000113 DAORQA010000000
VERSION     DAORQA010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3807_length_5441_cov_10.268102"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="PK736_06475"
     CDS             <1..1172
                     /locus_tag="PK736_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC33071.1"
                     /translation="INGTEKVRVLLAQALFGNPDILLLDEPTNDLDVETISWLENFLA
                     DFENTVIVVSHDRHFLDAVCTHVADIDFGKIQLFTGNYTFWYESSQLALRQRSDQNKK
                     AEDKRKELQDFIERFSANASKSRQATSRKKLLEKLVIEDIKASTRKYPGIIFKPERDC
                     GDQILQVEHLEKFSPEGAAFFTDVSFILNKGDKVAILSRDHLAINAFFDIITGEASAD
                     KGTVAWGSTITYSYLPVDNSPFFKGEDNLIDWLRQFSKDKDETYIRGFLGRMLFSGEE
                     TLKMSNVLSGGEKVRCMISRMMLTNANCLIINEPTSHLDMESITAFNNALKDWKHVAL
                     FSSHDHEFVQTSANRIIELTPSGILDKRVSYDEYITDENIKLQREKMYGDSVFSS"
     gene            1547..1939
                     /locus_tag="PK736_06480"
     CDS             1547..1939
                     /locus_tag="PK736_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRC33072.1"
                     /translation="MATIEKLEEIISWQKSLKVVKLIYQETNNEKFSRDFGLKDQIRR
                     SAVSIPSNIAEGFGRGGNKEFIQFLSIAKGSTFELKTQLLIAKDLDYIDEKQFMFIHK
                     ELDDIGKLLTAFQSYLKQSEMKGEKFVR"
     gene            2255..5233
                     /gene="uvrA"
                     /locus_tag="PK736_06485"
     CDS             2255..5233
                     /gene="uvrA"
                     /locus_tag="PK736_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRC33073.1"
                     /translation="MNFEDLSPKEFIIIKGAAVNNLKKIDVAIPRNKMVVVTGVSGSG
                     KSSLAFDTLYAEGQRRYVESLSSYARQFLGKMDKPEVDYIKGIAPAIAIEQKVNTRNP
                     RSTVGTATEIYDYLKLLYARIGVTYSPVSGLPVQKHNVQDVIDAIHKMPNDIALLIVA
                     PVEVPNATTHEILSNLQQKGFVRVLHHNHLVKIEDLLPLFENEKSAKKAKPKKGDQEL
                     PVIETEKKATKKTKTKKGETVEENKSEITDDNAIEIKVVVDRIMTAEHTDEYNARLAD
                     SVQTAFYEGRGTCYIAKLEDGNAVNFQQFSNKFEADGITFEEPTANLFAFNNPYGACK
                     RCEGFGSIIGVDPDLVIPDKSKSVYEGAIVCWVGEKMSTWLQNLIMTSFKFDFPIHNP
                     YYDLTAENKQLLWTGNKYFQGLNAFFEELEKQSYKIQYRVMLSRYRGKTVCPDCNGTR
                     LRADANYVKIDGIPISQLVLMPVQESYLHFKNLKLNAQQQVIAKRLLLEITHRLQYLN
                     DVGLGYLTLNRLSNTLSGGESQRINLATSLGSSLVGSMYILDEPSIGLHPRDTERLIT
                     VLKSLQKLGNTVIIVEHDEAIMRSADQLIDIGPFAGVHGGHLVFQGHYKDILDDEQSL
                     TAKYLTGKETINIPRIRRRWKNYIQIAGARENNLKNVTAKFPLNILTAVTGVSGSGKT
                     SLIKKILFPALKKMHGGYGEATGNFDRITGDYNMISDVEMIDQNPIGKSSRSNPVTYV
                     KAYDEIRQLYADLPLSRTRRLKTSHFSFNVDGGRCETCQGEGFVTIEMQFMADIRLKC
                     DACKGKRFKEEILSIKYRDTSIDQILDMTVDEALDFFDGIQTLEKFKNAEKKISNRIQ
                     PLADVGLGYITLGQASSTLSGGEAQRIKLASYLTRGHSATGMLFIFDEPTTGLHFHDI
                     SKLLDAFNALIKQGNTVIVIEHNQEIIKCADWVIDLGPEGGDAGGNIVFEGTPEDLLK
                     CNESYTAKFLKEKFEGVEEK"
BASE COUNT         1810 a          941 c         1131 g         1559 t
ORIGIN      
        1 atataaatgg caccgaaaaa gttcgcgtgc ttttggcaca agcgcttttt ggcaatcccg
       61 atattttgtt gctcgatgaa cctaccaacg atttggatgt agaaacaatt tcgtggctcg
      121 aaaatttcct tgccgatttt gaaaacactg taattgttgt atcgcacgac aggcactttc
      181 tcgatgctgt gtgtacacat gtggccgata tagattttgg aaaaatacaa ttgtttaccg
      241 gcaactatac tttttggtac gagagtagcc aacttgcatt gcgccaacga agcgatcaaa
      301 ataaaaaggc tgaggataaa cgaaaagaat tacaagattt tatcgaacgc tttagtgcca
      361 atgcatcgaa atcgaggcag gcaacaagcc gtaaaaaatt gctggagaaa ttggtgattg
      421 aagatataaa agcaagtaca cgaaaatatc cgggaataat atttaagccc gagcgcgatt
      481 gtggcgatca gattttacaa gtagaacatc tcgaaaaatt ttcgcccgag ggtgcagcat
      541 tttttaccga tgtaagtttt attcttaaca aaggcgataa ggtggcaatt ctatcgcgcg
      601 atcatttagc catcaatgca tttttcgata tcattaccgg tgaagcctct gctgataaag
      661 gaacagtagc atggggcagc accatcactt attcatacct gccggttgat aacagtccat
      721 tttttaaagg cgaagataat ttgatagact ggttgcgtca gttttcgaaa gataaagatg
      781 aaacatatat ccgcggattt ttgggacgta tgttattcag tggcgaagaa actttgaaaa
      841 tgtcgaacgt gctttcgggt ggcgaaaaag tgcgctgcat gattagccgt atgatgctta
      901 ccaatgcaaa ttgcttaatc ataaacgaac caacaagtca cctcgatatg gaatccatca
      961 ctgcattcaa caatgcattg aaagattgga aacatgtggc gctgttctca tcgcacgatc
     1021 atgagtttgt gcaaacatcg gccaatagaa ttattgagct tacgccctcg ggcattctcg
     1081 acaaacgtgt atcatatgac gaatacatta ccgatgaaaa tataaaattg caacgcgaga
     1141 aaatgtatgg ggattctgtt tttagttctt agtttttagt tcttggtttt tagttcttgg
     1201 tttttagttc ttagttacgt ttgcgatgcg gcatgttctt agttcttcgt ttcgtttgcg
     1261 gggaggattg ttcttggttc ttagttacgt ttgcggggag gattgttctt agttcttggt
     1321 ttcgtttgcg atgcgccttg ttcttagttc ttcgtttcgt ttgctatgtg ccttgttctt
     1381 cgtttcgttt gcgattaaat atccaatcga tcaaattaaa aatttttatt ttgtcaatta
     1441 accttagttg tttcaagttg aaattggttt tcaaaatata tggaaattat ttatagagtg
     1501 tggtttacga tattcaacat agtattttta aaatgaaaaa ttaattatgg ctacgataga
     1561 aaaattggaa gaaattattt cttggcaaaa gtcattaaaa gttgtcaagc ttatttatca
     1621 agaaacaaat aatgaaaaat ttagtcgcga ttttggtttg aaagatcaaa ttagaaggtc
     1681 ggcagtttct atcccttcaa atattgccga aggttttggc cgaggtggca ataaagagtt
     1741 tatacaattt ctctccatag ctaaaggttc tacatttgag ttgaaaacac aattgctaat
     1801 tgcaaaagat ttagattata ttgacgaaaa acaatttatg ttcattcata aagaacttga
     1861 cgatattggc aaattattaa ctgcatttca atcgtattta aaacaaagcg aaatgaaagg
     1921 cgaaaagttt gttagataaa tttcatgttc ttcgtttcgt ttgcgggggg ttgttcttag
     1981 ttacacattc aatgagccat gttcttagtt cttcgtttcg tttgttatga acaaaaaacg
     2041 aagaacaagg tgaacaataa aacgtaacga agaacgaaga acaaaaaacg aagaacaaga
     2101 atttgcattc gcatttaaca aagaacaagg tgaacaaaaa aacaaatcac aagaatttgc
     2161 attagcaatt aaccaagaac aaggtgaaca ataaaacgta acgaagaacg aagaacgaaa
     2221 aaacaaaaaa acaaaaaaca agaattttaa aaagatgaat ttcgaagatt taagtcctaa
     2281 agaatttata ataataaaag gagcggctgt taacaatctc aaaaaaattg atgtggccat
     2341 acctcgcaat aagatggttg tggttaccgg tgtgagtggt agtggcaaat catcattggc
     2401 tttcgatacc ttgtatgccg aagggcagag gcgttatgtg gaaagtttat catcgtatgc
     2461 gcggcagttt ctgggcaaaa tggataagcc cgaagtagat tatataaaag gcattgcacc
     2521 tgctattgca attgaacaaa aagtaaacac ccgcaacccc cgcagtacag ttggcaccgc
     2581 aacagaaatt tacgattact taaaattgct gtacgcccgt attggtgtaa cctattcacc
     2641 tgtgagtggg ctaccggttc aaaagcacaa tgtgcaggat gtaattgatg ctatacacaa
     2701 aatgccaaat gatattgcac tcctgatagt agcacctgta gaagtaccca atgcaaccac
     2761 acacgaaata cttagcaact tacaacaaaa aggttttgtg cgtgtattac atcataatca
     2821 tcttgtaaag attgaagatt tgttgccact ttttgaaaac gaaaaatcgg caaaaaaagc
     2881 aaagcccaaa aaaggtgatc aagaattgcc tgtaattgaa accgaaaaaa aagcaacaaa
     2941 aaaaactaag acaaaaaaag gtgaaactgt agaagaaaat aaatcggaga ttactgatga
     3001 taatgcaatc gaaattaaag ttgtggttga cagaataatg acagccgaac ataccgatga
     3061 atacaatgcg cgccttgccg atagcgttca aacagcattt tacgaaggac gtggcacttg
     3121 ttacatcgca aaattagaag atggaaacgc tgtaaatttt cagcagttca gcaataagtt
     3181 tgaagccgat ggtattacat tcgaagaacc tacggcaaac ttattcgcat tcaataatcc
     3241 atacggtgca tgtaaacgtt gcgaaggttt tggcagcatc attggtgtcg atcccgatct
     3301 tgttattccc gataaatcta aatccgttta cgagggtgct attgtttgtt gggtgggcga
     3361 aaaaatgagc acatggttgc aaaatttaat catgacaagt tttaagttcg attttccaat
     3421 tcacaaccca tattatgatc ttactgccga gaacaagcaa ctattgtgga ctggcaataa
     3481 atattttcag gggttaaatg cttttttcga agagctcgaa aagcaatcat acaaaattca
     3541 atatagggta atgttatcgc gctaccgagg aaaaacggtt tgtcccgatt gcaatggtac
     3601 tcgcctgcgt gccgatgcca actatgtaaa aattgacggc attcctattt cgcaattggt
     3661 gttgatgccc gtacaagaaa gttatttaca ttttaaaaat ttaaaactta acgcacagca
     3721 acaagtaatt gcaaaacgat tgcttctcga aattacacat cgacttcaat atttaaatga
     3781 tgtgggattg ggttatctta ctttaaaccg tttatccaac acattaagtg gaggcgaatc
     3841 gcaacgtata aatcttgcaa catctcttgg cagttctttg gtaggaagta tgtatatttt
     3901 agatgagccc agtattggtt tacatccgcg cgataccgaa cgattgatta ccgttttaaa
     3961 atcgttacaa aaactgggca acacagttat tattgtagag catgacgaag ccattatgcg
     4021 cagtgccgat caattgatag atataggacc ctttgccgga gtgcatggtg ggcatttggt
     4081 ttttcaaggt cattacaagg atatacttga cgatgaacaa agtcttacgg caaaatattt
     4141 aacgggcaaa gaaactatta acataccccg tatacggagg aggtggaaaa attatattca
     4201 gattgccgga gcccgcgaaa acaatttgaa aaatgtaaca gcaaaatttc cattaaatat
     4261 tttaaccgct gtaacaggtg tatccggttc gggaaaaact tcattgatta aaaagatttt
     4321 atttccggca ttaaaaaaaa tgcatggcgg ttatggtgag gccacaggta atttcgaccg
     4381 cataacgggc gactacaata tgataagcga tgtagaaatg atagaccaaa atccgattgg
     4441 aaaatcatcg cgcagcaacc ctgttactta tgtaaaagcc tatgatgaaa tacgtcaact
     4501 gtatgccgac cttccgcttt cgcgtacacg cagattaaag acttcgcact tttcttttaa
     4561 tgtggatggt ggccgttgcg aaacttgtca gggcgaaggt tttgtaacta tcgaaatgca
     4621 gtttatggcc gacatacgtt tgaaatgcga tgcatgtaaa ggcaaacgat ttaaagaaga
     4681 aattttaagt ataaaatacc gcgacaccag tatcgatcaa atattggata tgaccgtgga
     4741 cgaagccctc gatttttttg atggtataca aacactcgaa aaatttaaaa atgccgaaaa
     4801 gaaaattagc aatcgtatac aaccactggc cgatgtagga ttgggttaca ttacacttgg
     4861 ccaggcaagc agcacattaa gtggaggcga agcgcagcgt ataaaactgg catcgtatct
     4921 cactcgtgga cattcggcaa cgggcatgct ttttattttc gatgagccta ctacaggttt
     4981 gcactttcac gatataagta aactcctcga tgcttttaat gcattaataa aacaaggcaa
     5041 caccgtcatt gtaattgagc ataatcagga aataataaaa tgtgccgatt gggtaataga
     5101 ccttggcccc gaaggtggcg atgccggtgg caacattgtg ttcgaaggca cccccgaaga
     5161 tttactaaaa tgcaatgagt cgtacacagc aaaatttttg aaagaaaagt ttgaaggagt
     5221 ggaggagaag taattgttga gaaatataat ttcctttttt attcggcaaa aggtcttatg
     5281 attaactcca tcatttatga tggggcttag ctcaaacaga gtataggctt cagccatgaa
     5341 ataagctaac ccaaaaaaga gtcatagcga aagtcacgta tatagccatt gttgtaactc
     5401 tatcctaaat gacggggttt gtcaaagttg agatttcgat g
//
LOCUS       DAORQA010000114         5344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3882_length_5344_cov_6.703914, whole genome shotgun sequence.
ACCESSION   DAORQA010000114 DAORQA010000000
VERSION     DAORQA010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3882_length_5344_cov_6.703914"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(345..1595)
                     /gene="fabF"
                     /locus_tag="PK736_06490"
     CDS             complement(345..1595)
                     /gene="fabF"
                     /locus_tag="PK736_06490"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRC33074.1"
                     /translation="MQLKRVVVTGLGALTPLGNNVTNYWDALLKGVSGCGPITKFDAT
                     KFKTRFACEVRNFDAGDYIERKEIRKMDAFSHYAIAVCDEAVKDAGMLNNAAIDPDRV
                     GVVWGSGIGGLRTFQDEVSAFATGDGTPRFNPFFIPRMIADIACGHVSMRFGYRGPNY
                     TTVSACASSTNAIIDAFNLIRLNKADIIITGGSEAAVNEAGVGGFNACMALSMRNDSP
                     ETASRPFDKDRDGFVLGEGGGAVILEELEHALARGAKIYCEVGGGGMSADAHHITAPH
                     PEGLGAMNVMKNALSDAEMPFDQIDYINVHGTSTPLGDLAESKAIVRVFGDHAYNLNI
                     SSTKSMTGHLLGAAGVIEGIAAILAVKNDIVPPTINHFTDDSEFDPKLNLTFHTPVKR
                     IVRAALSNTFGFGGHNASVIFKKY"
     gene            complement(1635..1871)
                     /locus_tag="PK736_06495"
     CDS             complement(1635..1871)
                     /locus_tag="PK736_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRC33075.1"
                     /translation="MSEIDARVKAIIVDKLGVDEAEITKESSFTTDLGADSLDTVELI
                     MEFEKEFNIAIPDDQAEKIQTVGQAIDYISANIK"
     gene            2379..2729
                     /locus_tag="PK736_06500"
     CDS             2379..2729
                     /locus_tag="PK736_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33076.1"
                     /translation="MSQESLYFPLWQKYLPIIALQMKNAKNGEREITLYKTEFKEVGD
                     RKVSDFSFNLEIKNGRVASNIRGTAAARGLYEKLTQNKATKQLLTENYYKIQLFKDYK
                     MKIERIPQAEEEAV"
     gene            complement(3242..>4004)
                     /locus_tag="PK736_06505"
     CDS             complement(3242..>4004)
                     /locus_tag="PK736_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016193970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC subfamily B transporter ATP-binding protein"
                     /protein_id="HRC33077.1"
                     /translation="IAIDKKYLLHGEVRFQNVAFSYPSRKDVTVLKDVSLSASNGEQI
                     AIVGPSGAGKSTITSLLLRFYEPDAGQIFFDNRPSVDFPLSQLRGQMAYVPQDVILFG
                     GTIKENIAYGKPDASDAEIIEAAQKANAHEFVARFPEKYDTIVGERGIKLSGGQRQRI
                     AIARAILKNPAILILDEATSSLDSESEQLVQDALEYLMKGRTSFVIAHRLSTVRNAHK
                     IIVIDKGIVTESGTHNELMSKDGLYRRLSEMQFEI"
     assembly_gap    4005..4041
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4042..5120)
                     /locus_tag="PK736_06510"
     CDS             complement(<4042..5120)
                     /locus_tag="PK736_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRC33078.1"
                     /translation="MAERRGNFNSNGKQKEESPKVKISKESLREALILFTYIKPYRWK
                     FILSLVFIALSAFTTSMFPFLLGKMIDAASPGSTVPGMSGTVSKQFGFGLNAIEWTLD
                     TTLILIFVQLGIQTIFSFMRVYLLTEVGEKSLADMRRDVYSKLLTMPMSFFTEKRVGE
                     LSNRISSDLSQIQDAISFTLAEFLRGIFTLVIGLCFIFWISTKLALVMLAVVPVIAIL
                     AVVFGMRIRNMARKAQDQLADSGTIVQETFQGISVVKAFTSEFYEIQRYVKSIYAVVD
                     TAISNARFRGAFISFMIFSVFGTIAFVMWFGANMIKAGELKIGELTMFVIFSMFVGGT
                     FAGFADMFSQMQKILGATQSIREILR"
BASE COUNT         1592 a         1116 c          981 g         1618 t
ORIGIN      
        1 tggttttgtc tttgatttaa tttatatgta ttagtgataa tgttcttcct taattacttg
       61 gggttttgca ctaatttatc caaaattgaa ataaggttcc tcctgaatga ctttgggtgg
      121 ttttggtttt gaattaactt atctaaagta gtgataaggt tcctcctgaa tgacttgagg
      181 tggtgtacaa atttatctaa agtaaaaata agtttcctac tgaatgactt ggggtgttga
      241 acaaatttat ctaaagtaga aataagtttc gtcctgcatg acaagaggtg gctggtttgc
      301 gccagcaact aacaactgta aactaaacaa ctaaaaactg ccccttaata cttcttaaaa
      361 atcacactgg cattatgccc gccaaaacca aacgtattgc tgagtgcagc gcgcactata
      421 cgttttaccg gagtgtgaaa tgtgaggttg agtttaggat caaactctga gtcatctgtg
      481 aagtggttta ttgtaggagg aacaatatcg tttttaacgg caagaattgc agctatgcct
      541 tctataacac ctgcagcacc aaggaggtgg cctgtcatcg attttgtgct actaatatta
      601 aggttgtaag catggtcacc aaatacgcgc acaatggctt ttgattcggc caggtcacca
      661 agaggtgttg atgtgccgtg tacgtttata taatcaattt ggtcgaaagg catttcggca
      721 tcgcttaggg catttttcat tacattcata gctcctaaac cttcgggatg tggtgctgta
      781 atatgatgag catcggcact cataccgcct ccgcctactt cacaatatat ttttgctcca
      841 cgtgccagag catgttcaag ttcttctaaa ataacagctc caccaccttc gccaagcaca
      901 aaaccatcgc ggtctttatc gaaagggcgc gaagcggttt cgggcgaatc gttgcgcata
      961 ctaagagcca tgcaagcatt aaatccacct actccggctt cgtttacggc agcctcgcta
     1021 cctcccgtaa ttattatgtc ggctttgttt aagcgtatta agttgaaagc atctattatg
     1081 gcattggtac tcgaagcaca agctgaaaca gtggtatagt taggtccgcg atagccaaag
     1141 cgcatggata cgtgtccaca ggcaatatca gcaatcatcc tgggaataaa gaatggattg
     1201 aagcggggag ttccatcacc ggttgcaaat gcagatacct catcctgaaa agtacgaagt
     1261 cctccaatac ccgaacccca aactacgcca acacggtcgg gatcaatagc ggcattgtta
     1321 agcataccgg catcttttac agcttcgtca caaacagcta tggcataatg cgaaaaggca
     1381 tccatcttac ggatttcctt tctttcaata taatcaccgg catcaaaatt acgtacttcg
     1441 caagcaaagc gggttttaaa tttcgtggca tcgaattttg tgattgggcc acaaccgctc
     1501 acgcctttta acaatgcatc ccaataattg gtaacgttgt ttcccaaagg agtaagtgcg
     1561 cctaatccgg taactactac acgtttaagt tgcatcggct ttgctaataa atggagatgg
     1621 aaaagtcttt tgtgctattt aatattggca gaaatataat caattgcctg gcctacggtt
     1681 tgtatctttt cggcctgatc atcaggaatg gcaatgttaa actccttttc aaattccatt
     1741 atcaattcaa cggtgtctaa cgaatcggca ccaaggtcgg tagtaaaact cgactctttt
     1801 gttatttctg cttcatctac gcctaattta tctactatta tggcttttac gcgtgcgtct
     1861 atttctgaca ttttttatat aggtttttat tataagtcgg caaatgtaat aaaaatgaaa
     1921 taggtgcaaa aaatattttt caagcaccca ttaaacttaa ttttagaggt taaccatttt
     1981 tgaagggtaa tattttcaaa cccaaattgt attgaagtgc accaagtcaa atatatttat
     2041 aaacttattt cttgatgtgg aataaaatgc tttcttttgc cgtctccttt gccactctaa
     2101 attcggtttt aattgcttat accggtttaa attgattaaa gtaaaacctt acattaattc
     2161 gacaatttgg caatattgat tcttggcctg cattgttcca gtataactct tgtttctaaa
     2221 attacaggat ttaaataaag ataaaagtgg ctatggcggc ttatgtggaa taatgtgtaa
     2281 aacaggcttt gttgcaaaag caatagatat tatttgtaac aaacacaaaa accgggggtg
     2341 gaattttgaa aattgaaatt gaataaaaca atatagctat gagtcaagaa agtttgtatt
     2401 ttcctttatg gcaaaagtat ttgccgataa tagcgttgca aatgaaaaac gctaaaaatg
     2461 gagagcgcga aattaccctt tacaaaacag agtttaaaga ggtaggtgac cgcaaagtat
     2521 cggacttttc ttttaacctc gaaataaaaa acggtcgtgt tgccagcaac attcgtggca
     2581 cagcagcagc ccgtggttta tacgagaaac tcactcaaaa caaggctacg aaacaattgc
     2641 ttaccgaaaa ctattacaaa attcaactct tcaaagatta caaaatgaag attgaacgca
     2701 ttcctcaggc tgaggaagag gctgtttagt tattatttat tatttagttg ttgggttacc
     2761 aagctatttg gtaagccttt gtttagtttc cagaattaat ttttcgaatt aattttctga
     2821 tgaataacct ttacttttca atattacttt tttgccgggc tcacatttgc cttgtgaaca
     2881 attattacct tttctccgtt agcatatttt atcttttgtt atcgactgag atgtttatta
     2941 tagccttata atattcgatt cacaattcag aagtgtcggg acaaataact cagaacaaaa
     3001 aactgagtcc aaagcagcga ttgtttcgca gttcgagaat actcactgtg acaaatcgca
     3061 atgacgaact aaaaatgccg acactacggt cggcatcccg accgtagtgc caggactaag
     3121 aactgaatgt gaaatgcttg catcccgacc gaagtgtcag gactaaaaac tataaactga
     3181 atttgaactg aaaactgaat tcaaaaaact ataaactgaa tttcaaacta aaaactgaaa
     3241 actatatttc aaactgcatt tcgctcaacc ttctgtacaa tccatctttg ctcatcaact
     3301 cgttatgtgt tccgctttcg gttactattc ctttgtctat tacaattatt ttgtgggcat
     3361 tgcgcactgt actaagcctg tgtgcaatta caaacgatgt gcgtcctttc atcaaatatt
     3421 ctaatgcatc ttgtactaat tgttcgctct cactgtcgag cgatgaggtg gcttcgtcaa
     3481 gaataagtat tgcgggattt tttaaaatgg cacgtgcaat agcaatgcgc tgacgttggc
     3541 ctccgctcaa ttttattcct cgctctccaa ctatggtatc gtacttttcg gggaaacgcg
     3601 ctacaaactc atgtgcattg gctttttgtg cggcttcaat aatttcggca tcgctggcat
     3661 cgggttttcc ataggcaata ttttctttaa tggtacctcc aaataaaatt acatcttgtg
     3721 gcacataggc catttgtccg cgtaattgcg atagtggaaa atcaaccgaa ggtctgttat
     3781 caaaaaatat ttgcccggca tcgggttcat aaaatcgcaa cagcaacgat gtgatggtac
     3841 tcttgcccgc accacttggg cctactatgg ctatttgctc gccattgctt gcagataatg
     3901 atacatcttt tagtaccgtg acatccttgc ggctagggta cgagaaagcc acattttgaa
     3961 aacgcacttc gccatgcagc aagtattttt tatcgatagc aatannnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn ncgcaatatt tcgcgaatgc tttgtgtggc gccaagtatt
     4081 ttttgcattt gcgaaaacat atcggcaaac cctgcaaaag tgccgcccac aaacatgctg
     4141 aatataacaa acatggtaag ctcgccaatt ttcaactctc cggcttttat catattggca
     4201 ccaaaccaca ttacaaaagc tatagtacca aacacactaa aaatcataaa cgaaataaaa
     4261 gcaccgcgga aacgcgcatt cgaaattgcc gtatcaacca ccgcataaat actttttaca
     4321 tatcgctgta tttcgtaaaa ctcgctggta aaggccttca ccaccgatat gccttgaaat
     4381 gtttcctgaa caatggtgcc gctatcggca agctggtctt gcgctttgcg cgccatattg
     4441 cgtatacgca taccaaaaac cacagccagt atagcaatca caggcactac ggccagcatc
     4501 accaaggcaa gttttgtact tatccaaaaa ataaaacata atccaataac gagcgtaaaa
     4561 ataccgcgca aaaattcggc aagtgtgaac gaaatagcat cttgaatttg cgataaatcg
     4621 ctcgaaatgc gattgcttaa ttcgcctact cttttctcgg taaaaaaact catgggcata
     4681 gtaagcaatt tgctatacac atctctgcgc atatcagcca gcgatttttc accaacttcg
     4741 gtaagtaagt acacgcgcat gaacgagaaa atagtttgta tacccaactg cacaaaaatc
     4801 aaaattaacg tggtgtcgag tgtccattct atggcattta aaccaaaccc aaattgcttg
     4861 cttactgtgc cactcatgcc cggcaccgtg gacccgggcg atgcagcatc aatcatttta
     4921 ccaagcaaaa aagggaacat acttgtagta aatgccgaca atgctataaa cacaaggctc
     4981 aaaataaatt tccatcggta aggctttatg taagtaaaca aaatgagcgc ttcgcgtaaa
     5041 ctctccttcg atatttttac cttaggcgat tcctcctttt gttttccgtt gctgttaaaa
     5101 tttcctctgc gctctgccat tttttatttt caatcttttt ttaaaaccct tttccaattc
     5161 ttacccagcc agcgtttttc cattcaaccc acaaatcact aaataatatc tcctctattc
     5221 aatgtttatt ttgcttgctt caacttttca aatccatttc aatatttaaa aaccaaattc
     5281 ttttttattg cgggaggcgg tggcctctcc ggtatactga tgccttgcat cagtatacaa
     5341 atac
//
LOCUS       DAORQA010000115         5327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3903_length_5327_cov_15.992413, whole genome shotgun sequence.
ACCESSION   DAORQA010000115 DAORQA010000000
VERSION     DAORQA010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3903_length_5327_cov_15.992413"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..824)
                     /locus_tag="PK736_06515"
     CDS             complement(117..824)
                     /locus_tag="PK736_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HRC33079.1"
                     /translation="MENNQSRRNFIKATAIIGAGALLANTKLSALAPGDVPFATLPAL
                     PYDYTALEPHIDMLTMQIHHDKHHKTYVDKLNEAMAKETFATPPSLESLFETVSTRPA
                     AIRNHGGGHYNHSMFWQIMTPKHTDDIPGKLKVAIDASFRNVDELKKQMNEAASKRFG
                     SGWAWLIQQKDRLVVSSTPNQDNPLMDVAEVKGQPLIGIDVWEHAYYLKYQNKRGEYL
                     DAWWNVVNWNEVAKRMR"
     gene            complement(1022..5296)
                     /locus_tag="PK736_06520"
     CDS             complement(1022..5296)
                     /locus_tag="PK736_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRC33080.1"
                     /translation="MRPTSNFIKALNHIETNIKSELIVIAGDAQGEGFWKNIKIFLLD
                     LFYQENNDIVVNTESMSRGTNRIGGHKLFFEQRSDVDHFHYFMNPTSQKIIMEALLGS
                     ASEARGFIQKNAPQTVGEMEMVIPGSRSGKSKEGQPVLYVLPGIMGSHLEDDGDRVWL
                     NLMRMATGEMDRIKIENKKVTANGLVAKFYKDIVNYFNSDYYVVPFAYDWRKSVYDAA
                     DKLKTDIDDTIAKTDKSISFIAHSMGGLVVRAFAVKHPQLWKKLCDRPGTRVVMLGTP
                     LGGSFVVPRLFLGIGKTINVIATVDLAHSKKALLTQFVRYHGLLQLLPADGSQSFEDT
                     AIWNKMMTTAQSDYPVPASSDLSEYKKMLDKNFTEFQWNTDIFKYVAGKDDETPCRME
                     IDEGTNKVNFMSTPAGDGTVTWGSIPEGLQASTYYFEAEHGKLAAAEEAFAGIKELIQ
                     IGNTSLLSKTKPIMRGAAKISVMSPVETVTDVNEKDFADNIFGVKIQKPKVVSHQVRI
                     SITHGDMSHALYPVVVGHFENDAIVKAEKVLNQKVNNYFGIRYEANNYPGEIGTHDYV
                     LNNNYKPPGGIVVGLGHFGTLTENNLVRTLKQGFISYLITCKEDNFYASGEKLPGISC
                     LLIGTGFGGLNIYSAIKATLTAAKTANDFFIEAGYIDYPSIAHIEFIETYQHKAIQAG
                     RIINSLLATNSIYNNFVFSPDIIKKVSGSRIDIPDDFQIDWWHRLKVAESEATNGNDK
                     RTRVIKFSSITDKALSEDEVLSTNIKIVDRLIKEAAAYTNNDKALCETLYEMLIPNQF
                     KGYGSDLRNIVLIVDKETARYPWEMLRDAFSGHNDPIVTKAGFLRQLSSTPSRVPTKL
                     STTNNALVIGDPPLDNFFPQLPGAKAEAKEVVNLLREKEYDVVDFTNSNPGHEIIKSL
                     YTNSYRIWHIAAHGVVNDKTTGQTGIVLGRELILTPSDLKQVRNIPEIVFVNCCSLGT
                     INKEEEELLQRKFEVAAGIGTQLIEMGVKAVIVAGWEVDDAAAQCFSQTFYSQLLKGK
                     YYGESVRVAREITYTTYPSVNTWGAYQCYGDPYFTLDKSYTKKKRKTFTFVDKEEAKY
                     MLRNVVSNIETGTSIKEEVIEETNAIIEAVNKYPLWKGDAKIMELVAIIYKELGDYKE
                     AINWYRKLPGAEKASYSLRAVEQLFNLTTKQTYRDYNAGITAVTLANSSIIKRQAEID
                     NIKKQAIIEMEAYIHQFNQFNIAMTTERGSLLGSAYKNLAIITLPDATVYKKNILQAS
                     LSYKMAHEIYFNTNGKVYYYPFYNYHILNAILGILAPESKTAGKQHHKEGIAKQLMVS
                     AEVHAAEIDAINPDFWNKTAMSMQYLYEFISEKRESHLKGLKEKIFQNHMEARKVEGS
                     VNKQNAIGEHVVFLKKGFELATPAQVEKYFINKKIALLDLLLKELA"
BASE COUNT         1433 a         1103 c         1001 g         1790 t
ORIGIN      
        1 ttaattagag ccgatttttt tgtgtacaag ttattacttt ttgaaatata taaaatgcat
       61 taagggtttg agaataattg ttttcaaatt tttttgaaat gaagaaaata aacaacttac
      121 cgcatccgct tagcaacttc attccagttc accacattcc accatgcatc aagatattcg
      181 ccacgcttgt tttgatattt taaataatag gcatgttccc atacatctat tcctatcagc
      241 ggttgccctt ttacttcagc tacatccatg agtggattat cctgattggg agttgatgac
      301 actacaagtc tatctttttg ctgtattagc caagcccaac cactaccaaa tctttttgat
      361 gccgcctcgt tcatttgttt ttttaattca tccacatttc taaacgatgc atcgatggcc
      421 acttttaatt tacccggaat atcatctgta tgcttaggtg tcattatttg ccaaaacata
      481 ctgtgattgt aatgcccgcc tccatgattg cgaatggcag ccggcctggt agaaaccgtt
      541 tcgaataaac tctcgagcga aggaggtgta gcaaatgttt cttttgccat tgcctcgttc
      601 aatttatcta cataagtttt gtggtgctta tcgtggtgaa tttgcattgt taacatatct
      661 atgtggggct ccaatgcagt ataatcgtat ggtaacgcag gcaaagttgc aaagggtaca
      721 tcgcccggtg caagggcgct taattttgta tttgcaagta acgcaccagc accaataatt
      781 gctgttgctt taataaaatt tctgcggctt tggttatttt ccattttgaa tatttttaaa
      841 ttttctcaaa catacattta aaatttcaat gcaggttgta aaatgtatga agtccttttt
      901 ctaaagaaaa gacattagta aagcgtatgc aaatcaggca aaatcaatct ttaatctttt
      961 ttgacagcgt ctgcaacaaa cattttcttt ccttaaaata tttcaatcca ttcaaaaaaa
     1021 attatgcaag ttctttcaac agtaaatcga gcagggcgat ttttttattg ataaaatatt
     1081 tttcgacctg agcaggagtg gcaagttcaa agcccttttt caaaaacaca acatgttcac
     1141 ctatggcatt ttgtttgttt accgatcctt ccactttgcg ggcttccata tgattttgaa
     1201 aaatcttttc ttttaatcct ttgaggtgcg actctctttt ttcggaaata aactcgtaga
     1261 ggtattgcat actcattgcc gttttgttcc aaaaatcggg attgatggca tcaatttcag
     1321 cggcatgtac ttcggcactt accatcagct gtttggcgat gccttcttta tgatgttgct
     1381 tgccggcagt tttgctttcc ggtgctaaaa ttccaagtat ggcatttagt atatgataat
     1441 tgtaaaacgg ataataataa accttgccat tggtattaaa ataaatttcg tgtgccattt
     1501 tatatgacaa cgaagcctgc aatatatttt tcttgtaaac tgttgcatcg ggtagtgtta
     1561 ttattgccag atttttgtaa gccgaaccaa gcaaactacc acgctctgta gtcatggcaa
     1621 tattaaattg attgaactga tgaatgtatg cttccatctc gatgattgct tgcttcttaa
     1681 tattatcgat ttcggcttgt cgcttaataa tactgctgtt ggctagcgtc acagcagtga
     1741 tgccggcatt ataatcacgg tatgtttgct tcgttgtcaa attaaaaagt tgttctactg
     1801 cacgaagcga gtaactggcc ttttcggcac cgggtaattt acgataccag ttgatagctt
     1861 ctttgtaatc gccaagttct ttatatatga tagcaacgag ttccattatt tttgcatcgc
     1921 ccttccacag tggatattta tttacggctt caataatggc attggtttct tcaatcactt
     1981 cctccttaat gcttgtgcct gtttcaatgt tgctgacaac attccgtaac atatattttg
     2041 cttcttcttt gtcgacaaaa gtgaatgtct tgcgtttctt ttttgtataa gatttgtcga
     2101 gtgtgaaata tggatcgcca tagcattgat atgcgcccca ggtattaaca gatggatagg
     2161 tagtgtaagt aatttcgcgt gctaccctta ccgattcgcc atagtatttt ccttttaaca
     2221 attgcgaata aaatgtttgc gaaaagcatt gtgctgcggc atcgtccact tcccatccgg
     2281 ctactattac agctttcaca cccatttcaa tcagttgtgt accaatgcct gcggccactt
     2341 caaatttgcg ttgcagcaat tcttcttctt ctttattaat agtgccaagt gagcagcagt
     2401 ttacaaaaac tatttcggga atattacgca cttgtttcaa atcggaaggg gtgagtataa
     2461 gttcacgacc cagcactatt ccagtttgtc ctgttgtttt atcattcact acaccgtgtg
     2521 ctgctatatg ccaaatacga taactgtttg tgtacaggct ttttattatt tcatggccgg
     2581 gattgctgtt tgtaaaatct accacatcat attctttttc gcgcagcaaa ttcacaactt
     2641 ctttcgcctc cgcttttgca cccggtaatt gagggaaaaa attatcgagt ggcggatcgc
     2701 caattaccaa agcattattt gttgtagata attttgttgg cacgcgcgat ggtgtagaag
     2761 aaagttgccg caggaatccg gcttttgtta ctataggatc gttgtgtccg ctaaatgcat
     2821 cacgcagcat ttcccatggg tagcgcgctg tttctttgtc aacaatcaat actatatttc
     2881 ttaagtcgga accataccct ttaaactgat ttggaataag catttcgtaa agggtttcgc
     2941 acagtgcttt atcattattg gtatatgcgg ccgcttcttt aataagtcgg tctactattt
     3001 taatattggt tgaaagaact tcgtcttcgc taagtgcttt gtctgtaata gaactaaatt
     3061 ttattacccg cgtacgttta tcgttaccgt tagtagcttc ggactcggct actttgaggc
     3121 ggtgccacca gtcaatttga aaatcatcgg gaatgtcgat acggctaccc gaaaccttct
     3181 tgataatatc gggcgaaaaa acaaaattat tgtaaatgga atttgtagcg agcaacgaat
     3241 tgataatgcg gccggcctga atggccttgt gttgatacgt ttcgataaac tctatatgtg
     3301 caattgaagg ataatcaatg tatccggctt caataaaaaa gtcattggcc gttttagctg
     3361 ctgtaagtgt agcctttatt gcactgtaaa tatttaagcc gccaaatcct gtgcctataa
     3421 gcaggcatga tataccgggt agcttttcgc cactggcata aaagttatct tccttacatg
     3481 tgataaggta actgatgaac ccttgcttca aagtacgaac aagattattt tcggtgaggg
     3541 taccaaaatg cccaagtcct acaactatac caccgggtgg cttgtaattg ttattaagca
     3601 cataatcgtg ggtgcctatt tctcctggat aattgttagc ctcgtaacgt ataccgaaat
     3661 agttatttac tttttgattc aacacttttt cggcttttac aatggcatca ttttcaaaat
     3721 gaccaactac aacagggtaa agcgcatgtg acatgtcgcc atgtgtgatg gatatacgta
     3781 cctgatgcga aaccactttg ggtttttgaa tttttacacc aaatatatta tcggcaaagt
     3841 ccttttcatt tacatcggta acggtttcta cagggctcat cacgcttatt tttgcagcac
     3901 cacgcatgat gggtttcgtt ttcgatagca gactggtatt gccaatttgt atcagctctt
     3961 taataccggc aaaagcttct tcggcagcag caagtttacc atgttccgct tcgaaataat
     4021 aagtagaggc ctgcagccct tcgggaatac ttccccatgt aacagtgcca tcaccggccg
     4081 gtgtgctcat gaaatttacc ttgtttgtac cttcgtctat ttccatgcga caaggcgttt
     4141 catcgtcctt accggctaca tatttaaata tatcggtgtt ccattgaaac tctgtaaagt
     4201 ttttatcgag catcttttta tactccgata aatcgcttga tgcaggaaca ggataatcgc
     4261 tttgtgctgt ggtcatcatc ttgttccaga tagcagtatc ctcaaacgat tgactaccat
     4321 cggcaggcaa caattgtaat aaaccatgat agcgtacaaa ctgagtaagc aatgcttttt
     4381 tactatgtgc caggtcgacc gttgctatta cattgatggt ttttccaatt cctaaaaaca
     4441 aacgtggtac aacaaaactt ccaccaagag gtgtccctaa cattaccaca cgtgtacctg
     4501 gtcggtcgca aagttttttc cacaactgtg ggtgtttcac tgcaaatgcg cgcaccacaa
     4561 gccctcccat agagtgagcg ataaacgaaa tcgacttatc ggtttttgca attgtatcat
     4621 caatatcggt tttcaattta tcggcagcat catataccga tttgcgccag tcgtatgcaa
     4681 aaggtacaac ataataatcg ctattgaaat aatttacaat atctttgtaa aacttggcaa
     4741 caaggccatt ggcagtaact tttttgttct ctatttttat tctatccatt tcaccggtgg
     4801 ccatgcgcat taaattgagc cacaccctat cgccatcatc ttcgagatgg cttcccataa
     4861 ttccgggtaa tacatacagc accggttgtc cttctttcga cttgcccgag cgcgagccgg
     4921 gtataaccat ttccatttcg cctaccgtct gtggagcatt tttttgaata aaacctcgtg
     4981 cctcacttgc actaccgagc aacgcttcca ttattatttt ttgcgaagta ggattcataa
     5041 aataatgaaa gtgatctaca tcgctgcgtt gctcaaaaaa tagtttgtgg ccacctatac
     5101 gatttgtacc acggctcatc gattcggtgt ttactacaat gtcgttgttt tcctggtaga
     5161 ataaatcgag aagaaatatt tttatatttt tccaaaaacc ttcaccctga gcatcaccgg
     5221 caattactat gagttctgat tttatatttg tttcaatgtg attgagcgcc tttataaaat
     5281 tggatgtagg gcgcatgcac tcaatacctg gcagtgtatc gaaatcg
//
LOCUS       DAORQA010000116         5304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3917_length_5304_cov_17.691179, whole genome shotgun sequence.
ACCESSION   DAORQA010000116 DAORQA010000000
VERSION     DAORQA010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3917_length_5304_cov_17.691179"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            575..1510
                     /locus_tag="PK736_06525"
     CDS             575..1510
                     /locus_tag="PK736_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRC33081.1"
                     /translation="MAEIIIPLDITNPVAFFGANDANVDAIRKKFPALKIIARGNEMK
                     ILGEDDLANQCKLFIENLAYEYDKNGILTPVDDSPVFQANGNKTNGNASNVLVYGPNG
                     IVVKARTPNQIRMVEACEHNDILFAIGPAGTGKTYTAVAIAVRALKNKEVRKIVLCRP
                     AVEAGEHLGFLPGDMKEKIDPYLRPLYDALFDMIPGEKLRSMIENGIIEVAPLAFMRG
                     RTLDNAFVILDEAQNATESQLKMFLTRMGPSAKFIVTGDTTQIDLPPKQPSGLIQAIK
                     ILPNIAGIDIIFLNESDVVRHRLVKDIIMAYKNNH"
     gene            1528..2472
                     /locus_tag="PK736_06530"
     CDS             1528..2472
                     /locus_tag="PK736_06530"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRC33082.1"
                     /translation="MNLIEKTNFQFRNQTAYYKGKVREVYTINNTYMVMIATDRISAF
                     DVILPRAIPYKGQVLNQTASYFLNATKAIVPNWFIHSPDENVSIGYKCEPYKIEMVVR
                     GYLAGHAWRTYNSGLRTICGVKMPDGLIENDKLPEPIFTPTTKSAHGHDLDITKEEII
                     SQGIVSAADYEAIENYAGALFTEGSKMANKQGLILVDTKYEFGNHHGTVMLMDEIHTP
                     DSSRYFYLEGFDERQKNNQPQKQLSKEFLRQWLIENNFQGLEGQTVPQMDDDIVNAVT
                     NRYVELYQTITGLTFQHRNYDGALSVMEQKIIAALQSF"
     gene            2489..3169
                     /locus_tag="PK736_06535"
     CDS             2489..3169
                     /locus_tag="PK736_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRC33083.1"
                     /translation="MENDCAIIQSIREELKPYSAVLVAVSKTKPNSEIEKVYACGQRI
                     FGENYVQELVLKHEQLPADIAWHFIGHLQSNKVKYIAPFVSMIHSVDSENLLETINAQ
                     AAKNKRIIDCLLQVYIAKEETKFGVDESELQKIIENYLAHNYSHIRLRGLMGMATNTD
                     DMNIVQNEYKRLKSIFDTIALLIEKEKHFDTLSMGMTGDYKIALECGSNMIRIGSKIF
                     GERNYNKL"
     gene            complement(3164..3598)
                     /gene="aroQ"
                     /locus_tag="PK736_06540"
     CDS             complement(3164..3598)
                     /gene="aroQ"
                     /locus_tag="PK736_06540"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRC33084.1"
                     /translation="MKKKITIINGPNLNLTGRREQALYGMKSFESFLEELKVKYNNID
                     LQYYQSNVEGELINKLHQVGFEQHGIVINAGGYTHTSVAMGDAIKAIETPCVEVHISN
                     IMARESFRHQSYIAASCVGSIIGLGLKGYDLAIEFLIDYVPS"
     gene            3722..4621
                     /gene="xerD"
                     /locus_tag="PK736_06545"
     CDS             3722..4621
                     /gene="xerD"
                     /locus_tag="PK736_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRC33085.1"
                     /translation="MNYSSYIKGFEAWLKLEKSLSPNSIEAYIDDVNKLFAFLETNNL
                     MLQPAQLQITHLQEFLQGIGASGLSARSQARIISGVKSFFKYLLLENIIQDDPTTLLD
                     SPKLGRKLPDTLCVEEIDKLINAIDLSMPEGERNKALLETLYSSGLRVSELVNLKLSN
                     IFEEIGFLKITGKGNKERLVPIGKTALKQINYYKEKYRNHLMINAGHEDFLFLNRRGR
                     KLTREMIFTIIKTLAIKINLNKKISPHTFRHSFATHLVEGGADLRAVQEMLGHESITT
                     TEIYTHLNRDYLRENILKFHPRA"
     gene            complement(4717..>5304)
                     /locus_tag="PK736_06550"
     CDS             complement(4717..>5304)
                     /locus_tag="PK736_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33086.1"
                     /translation="DSPIGTGAVRLASDNKIYFSRAYESPNTWSFPYQDTMRNYINEN
                     LSVINEPDSLGAKCDYQPFSFHLGGKRTYYGLPNNPDYELGPLVGSPCDTLSANSPPA
                     PQRGEQKLYTTYIAAWEKLFINASGLKGKQLTATIYDATGKEKLARGKPPGGGLGVFG
                     GHFTLDVDCTGWSDGLYIVHLQTEKEMLSKKFVKE"
BASE COUNT         1713 a         1047 c          995 g         1549 t
ORIGIN      
        1 tcttagtccc gacacttcgg tcgggatgcc gaccaaagtg tcggcattct tagttacgtt
       61 cgctgagaga caagttctta gtcccgacac ttcggtcggg atgccgacca aagtgtcggc
      121 attcatagat acgtttgctg ggcgctatgt tcttagtttc gtttgcgaag tgtttataat
      181 ttcagagtct ctcatgcctt tgaaaaacaa gaaataaatg tggactctgg aattattagt
      241 taagttctta gtttcggaat acagttttta gttttttttt cgtcatggcg aggtacgaag
      301 caatctctgc gtagtacttt gtttcgtaaa ttaatttcag agtctctcaa gcctttgaaa
      361 caaaaaataa atgaggactc tgaaattatt tgttcttagt tttagttgaa tgcgtttaat
      421 attttcacaa ttcacaattc acaattcaca attcacaatt cgcaattcgc aattcacaat
      481 tcacaattca caattcacct ttaatctttc aactttaatc ttttgaataa aacttttgcc
      541 tttctttgtg atgtataatt aaaacagaaa agatttggca gaaattatta ttccacttga
      601 tatcactaat cccgtagctt tttttggcgc aaatgatgct aacgtggatg caatcagaaa
      661 aaaatttccc gcattaaaga taattgcccg gggcaacgaa atgaaaattc ttggcgaaga
      721 tgatcttgcc aatcaatgta aattgtttat cgaaaatcta gcctatgaat acgacaaaaa
      781 cggcatttta actcctgttg acgactctcc ggtttttcag gcaaacggaa acaaaacaaa
      841 tggcaatgca agcaatgtac tggtttatgg cccaaacggt attgttgtaa aagcccgaac
      901 tcccaatcaa attcgcatgg ttgaagcctg cgagcacaac gatattttat ttgcaatagg
      961 ccctgccggt acaggtaaga cttatactgc tgttgccatt gctgttaggg cattgaaaaa
     1021 taaagaagtg cgcaaaatag ttttatgccg ccctgcggtt gaagccggtg agcatcttgg
     1081 tttcttgccc ggtgatatga aagaaaaaat agatccttac ttacggcctt tgtacgatgc
     1141 actgttcgat atgattcccg gagaaaaatt gcgatcgatg attgagaatg ggattataga
     1201 agtggcacct cttgcattta tgcgtggccg aacgctcgat aatgcatttg taattcttga
     1261 cgaggcccag aatgctaccg agagtcagtt gaaaatgttt ttaacccgca tgggtccatc
     1321 tgcaaagttt attgttacag gcgataccac acaaatcgat ttgccaccca agcagccttc
     1381 gggacttata caggctataa aaattttacc taacattgcc ggcatcgata ttatattcct
     1441 caacgaaagt gatgttgtgc gccaccgcct ggtgaaagat attattatgg cttacaaaaa
     1501 taaccattag tatatacgta gtataaaatg aacctcatcg aaaaaacaaa tttccagttt
     1561 cgtaatcaaa ccgcctacta caaaggtaaa gtgcgcgaag tatataccat caacaataca
     1621 tacatggtaa tgattgccac cgaccgcata agcgcattcg atgtgatatt gccgcgtgct
     1681 attccataca aaggtcaggt gcttaatcaa acagcatcat attttttaaa tgctacaaaa
     1741 gctattgtgc ctaattggtt tattcactcg cctgacgaaa atgttagcat tggctacaaa
     1801 tgtgagccat ataaaattga aatggtggtg cggggatatt tggccgggca tgcatggcgt
     1861 acctacaatt caggcttgcg aacaatatgc ggagtaaaaa tgcccgatgg tttaattgaa
     1921 aacgataagc tacccgagcc catttttaca ccaaccacaa aaagcgcaca tggccacgac
     1981 ctcgatatta ccaaagagga aataatttca caaggcattg tttcggctgc agattatgaa
     2041 gcaatagaaa attatgctgg tgcattattt accgaaggat cgaaaatggc aaataagcaa
     2101 ggcttaattt tggtagatac aaaatatgag tttggcaatc atcatggaac agtgatgttg
     2161 atggacgaaa tacatacacc cgattcatcg cgttactttt atttggaggg atttgatgag
     2221 cggcaaaaaa acaatcaacc gcaaaagcaa ttgtcgaaag aatttttgcg acagtggtta
     2281 atcgaaaata attttcaggg gctcgaaggt caaactgttc cacaaatgga tgatgatatt
     2341 gtaaatgctg taacaaaccg ttatgtcgaa ttgtaccaaa ccattacagg tcttacattt
     2401 caacaccgca actatgatgg tgcgttatct gtaatggaac aaaaaataat tgctgcatta
     2461 caatcatttt aaaaacatat ttattttcat ggaaaacgat tgtgccatta ttcagtctat
     2521 tcgagaggaa ttgaaacctt atagtgcagt acttgtagca gtatcgaaaa ccaaacccaa
     2581 cagtgaaata gaaaaagtgt acgcttgtgg gcaacgaatt tttggtgaga attatgtgca
     2641 ggaactggtt ttaaaacacg agcaattgcc tgccgatatt gcgtggcatt tcataggaca
     2701 tttgcaaagc aacaaagtga aatacattgc accttttgta agcatgatac actcagttga
     2761 tagcgaaaat cttttagaaa ctattaatgc acaggcagca aaaaataaac ggattataga
     2821 ttgcttattg caagtatata ttgcaaaaga agaaaccaaa tttggagttg atgaaagtga
     2881 attgcaaaag attatcgaaa attatcttgc gcacaactat agccatatac gtttgcgtgg
     2941 gcttatgggt atggctacca ataccgatga tatgaatatt gtgcagaatg agtacaagcg
     3001 attgaagagt atattcgata ctatagcttt gcttatagaa aaagaaaagc atttcgatac
     3061 cctatcgatg ggcatgaccg gtgattataa aattgcactt gaatgcggca gcaacatgat
     3121 acgcatagga agcaaaatat ttggtgaaag aaattataat aaattatgat ggcacataat
     3181 ctatcaaaaa ctcaatagcc aaatcgtaac ccttaagtcc taaaccaata atagaaccta
     3241 cacaactggc agcaatataa ctctgatgcc ggaacgattc gcgtgccata atatttgata
     3301 tgtgaacttc tacacatggt gtttcgatgg ctttaatggc atcgcccata gccaccgaag
     3361 tgtgggtata tcctccggca tttattacta tgccatgttg ctcaaatcct acctgatgca
     3421 atttgtttat caactcgccc tccacattgc tttggtaata ctgcaaatca atattgttgt
     3481 attttacttt cagttcctct aaaaatgatt cgaaactttt catgccatac agggcttgct
     3541 ctctgcggcc ggtaaggttc aggtttggtc cgtttattat tgttattttc tttttcatat
     3601 tgtgattaca aatatacatt ttaaaatttt actgcataca catcgttatg ttgaaaatat
     3661 aaaacgcaca taaaatatcg ctagtatttt aagcctactt ttattgcaat aaaaaaatgc
     3721 agtgaactat tcatcataca tcaagggatt tgaagcctgg cttaagctcg aaaagagttt
     3781 aagtcccaat tctatagaag catatatcga tgatgtaaac aaactttttg catttctcga
     3841 aaccaataat ttaatgttgc aaccggcaca attgcaaata acccacctac aggaattttt
     3901 gcaaggcatt ggtgcatcag gtttaagtgc ccgatcgcag gcacgaatta tttcgggagt
     3961 aaaatccttt ttcaaatatt tgcttttaga aaacattatt caggacgatc ccactacatt
     4021 attagacagt ccaaaacttg gccgcaaatt gcccgacaca ttgtgtgtag aagaaattga
     4081 taaattaata aacgccatcg acctgagtat gcccgaaggc gaacgcaaca aagccttgct
     4141 cgaaaccctg tacagtagcg ggctacgcgt gagcgaactt gtaaatctga aactatcaaa
     4201 catatttgaa gaaattggtt ttcttaaaat taccggaaag ggaaataaag aacggcttgt
     4261 gcccattggc aaaacagcat tgaagcaaat aaattattac aaagaaaaat accgcaatca
     4321 tttaatgata aatgcaggcc acgaagattt tttattcctt aaccgcagag gacgcaaact
     4381 tacacgtgaa atgattttta ccattatcaa aactttggcg ataaaaatta atttaaacaa
     4441 aaaaatttct cctcacacct ttcgccattc gtttgcgaca cacttagtag aaggtggtgc
     4501 cgacctccgt gcagtgcaag aaatgctcgg ccacgaaagc attaccacca ccgaaatcta
     4561 tacacactta aacagagatt acctgcgcga aaatattttg aagtttcatc ccagggcgta
     4621 agtgttttca aaaaatatat tttgcaattc aacctgataa agaatttttg atttatggtt
     4681 tctgtaaatc gaaatattat ggtcacatat ttcattctac tccttcacaa acttcttact
     4741 caacatttcc ttctccgttt gtaaatgtac aatatacaac ccatcgctcc aacctgtgca
     4801 atcaacatca agtgtaaaat gcccgccaaa aacccccaaa cccccacctg ggggctttcc
     4861 ccttgcaagc ttttcttttc cagtagcatc gtatatggtt gccgttaatt gcttcccttt
     4921 caagccactt gcattaataa ataacttctc ccatgccgca atatatgtag tgtagagttt
     4981 ttgctccccc ctttgggggg cgggggggct gtttgcactc aaagtatcac aaggactccc
     5041 caccaacggc cccaactcat aatccggatt attaggcaac ccataataag tgcgcttacc
     5101 accaaggtga aaagaaaaag gctgataatc gcatttagca ccaaggctat caggctcatt
     5161 tattacactt aggttttcat ttatgtagtt gcgcattgtg tcttgatagg ggaaactcca
     5221 tgtattagga ctttcataag cacgcgaaaa atatatttta ttatcagaag ctaaacgcac
     5281 agcacctgta ccaattggag aatc
//
LOCUS       DAORQA010000117         5280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_3940_length_5280_cov_14.821818, whole genome shotgun sequence.
ACCESSION   DAORQA010000117 DAORQA010000000
VERSION     DAORQA010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3940_length_5280_cov_14.821818"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PK736_06555"
     CDS             complement(<1..791)
                     /locus_tag="PK736_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRC33087.1"
                     /translation="MKNIDSYTHTRGESIYLDDISEISGTLFGACFDSPVAHGIIKNL
                     DLSEAEKMNGVVKIFTHNDIPGRNEIGGIIEDEPLLADHHVHFCGMPIAFVVAETSEQ
                     AYAAIKKIKIEIEPLPVITDPRVAKEKNELIIPPRTFKIGDTASSFSTCDYIFEGRAD
                     SNGQEHLYIETQGAYTVPLENGAIKIYSSTQGPTAVQRACSKVLAIPMHQLEVDVTRL
                     GGGFGGKEDQANAWAALCALATYCTKRPVKYILNRMEDMRMTGKR"
     gene            complement(868..2298)
                     /locus_tag="PK736_06560"
     CDS             complement(868..2298)
                     /locus_tag="PK736_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRC33088.1"
                     /translation="MIKFILNDKEIATAEPPGLVLLDFIRYHKNLKGTKIGCREGDCG
                     ACTVLVGELIDGELRYRSATSCLMAIGNANNKHIVTIEGINMSGLNPIQQAMSDEGAT
                     QCGFCTPGFVMSLAGFCLSDKEATEKNVIASIDGNICRCTGYKSIERAAILVNELLLN
                     RNTQSSEQYAVKNKLLPEYFLNIKERLQNLIANNPITNNPVTNNEITNNPITNLGGGT
                     DLYVQKHDTMKDAEINFLFDNKKLKGIFIDGNNCFIGASCTVTDLMESKIIEKYFPHF
                     AQYSKLVSSTPIRNIATLAGNFVNASPIGDFTIFFLSLNTQLILSDGTNKREIALRNF
                     YNGYKSIDKKQEEYIEQFYFPLPDEGTKFNFEKVSKRTHLDIASVNTAMQIKIVGDKI
                     SGAIISAGGVFAFPKYLENASNYLSGKNISEENILCAIEMAQKEIAPISDARGSEEYK
                     RLLLSQLIKAHFIVLFPDKILANELI"
     gene            complement(2329..2811)
                     /locus_tag="PK736_06565"
     CDS             complement(2329..2811)
                     /locus_tag="PK736_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRC33089.1"
                     /translation="MIEHDSDKLFLGQAIEMAANGMRNNEGGPFGAVIVKDGIIVGRG
                     NNKVTSTNDPTAHAEVVAIRDACKNLNTFQLTGCTLYTSCEPCPMCLAAIYWARPDRV
                     VYGCSKEDAAAIEFDDAFIYNEIQLAISDRKMLMQQLNRDEAKKVFDEWAAKVDKTSY
                     "
     gene            complement(2903..3334)
                     /locus_tag="PK736_06570"
     CDS             complement(2903..3334)
                     /locus_tag="PK736_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC33090.1"
                     /translation="MIQQNENVFDLCLILLRGLPGSGKSTLAGILSEDGKYPCLSVDD
                     FFTDNATGQYNFEYDKNHFAYKQCEEQTDKQMENKSPKIFVHNTFTFDWEILPYIKLA
                     AAHKYKLFVVTVEKYHHDHNIHNVTQEQIEKMAQKYNVRLY"
     gene            complement(3362..4135)
                     /locus_tag="PK736_06575"
     CDS             complement(3362..4135)
                     /locus_tag="PK736_06575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRC33091.1"
                     /translation="MIITDTHIHLYSKEFDTDRDELVKTAMDKGVQRFFLPNIDFDSV
                     DPMFALTHKYPQNCFAMLGLHPCYITANCTDQLTQMEKLIDAHRKEIYAIGEIGLDFY
                     WDTTYVKEQEVAFIKQIHWASELNLPIAIHSRNATDEIIAILQQHHHLNLRGVFHCYS
                     GQPSQAQQIIDMGFYLGIGGVVTFKKAGLDVDMQTIDLKHVILETDGPYLAPTPYRGK
                     RNEPSYIVMVVQRIAEIKNISVEEVAQITTENSRQLFGI"
     gene            4183..5169
                     /locus_tag="PK736_06580"
     CDS             4183..5169
                     /locus_tag="PK736_06580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRC33092.1"
                     /translation="MQKTKILILRFSSIGDIVLTTPIIRCLKQQVPDCELHYFTKKQF
                     HTVIQHNPYIDKIHLLDDNMSELITELKNEKFDYVIDLHKNQRTWLLKFRLGVKSFTF
                     DKINREKFLITKLKIDVLPKEHIVDRYFEAVQKINVKNDEQGLDYFVAENDYVDLKKY
                     DIENGTKFIAIAIGAQHATKRLPTNKLIEICKKINSQVILLGGPTDVTTGIEIANACK
                     SNVINLCGELTLNQSAFVIKHAYKIITHDTGLMHIAAAMKKPIVSVWGNTIPQFGMYP
                     YYGNEIIESKIVEVKNLSCRPCSKLGYNKCPKYHFDCMNKIDVNEVVLFSND"
BASE COUNT         1569 a         1017 c          954 g         1740 t
ORIGIN      
        1 tgtcgctttc cggtcatgcg catatcttcc atacgattca aaatatattt caccgggcgt
       61 tttgtacaat aagttgccaa agcacataat gctgcccacg cattggcttg atcttcttta
      121 ccaccaaagc caccaccaag tcgtgtgaca tcaacctcga gttgatgcat tggaatagcc
      181 aacactttgc tgcatgcacg ttgcacagct gttggccctt gtgtactgga ataaattttt
      241 attgctccat tttctaacgg tactgtatac gcaccttgtg tttcaatata aagatgttct
      301 tgcccgttgg aatcggcacg cccttcaaaa atataatcgc aagtagaaaa tgatgatgca
      361 gtgtctccaa ttttaaaggt gcgcggtgga attatcagct cgtttttttc tttcgccaca
      421 cgcggatcgg taataacagg cagcggttct atttcaattt taattttctt aattgcagca
      481 taagcttgct cactcgtttc ggccacaaca aacgctatgg gcatgccgca aaagtgaaca
      541 tgatgatcgg ccagcaacgg ttcgtcttct attattccac ctatctcatt tcgtccgggt
      601 atatcattgt gcgtaaatat tttcactacg ccattcattt tttcggcttc cgataaatca
      661 agatttttta taattccatg cgcaacaggc gagtcgaagc aagcaccaaa caacgttccc
      721 gaaatttcgg aaatgtcatc gaggtaaata gattcgcccc gtgtatgtgt atatgaatcg
      781 atgtttttca ttttcatatt aatgaataat cggtaattat ttttccaaac caacgcaaca
      841 gaaaactact tttcgaaaac atattcatca gattaattcg tttgctaaaa ttttatcagg
      901 aaataaaact ataaaatgtg ccttgattaa ttgtgaaagc aaaagccgct tgtattcttc
      961 gcttccgcga gcatcactta ttggggcaat ttctttttgt gccatttcaa ttgcacataa
     1021 aatattttct tccgaaatat tttttcctga taaataattg cttgcatttt ctaaatattt
     1081 aggaaaagca aaaacacctc cggccgaaat tattgcgcct gaaattttat cgcccactat
     1141 ttttatttgc attgccgtgt tcacacttgc aatatcgaga tgggtgcgtt tacttacttt
     1201 ttcgaaattg aattttgtgc cttcatcggg taaagggaaa taaaattgtt cgatgtactc
     1261 ttcctgtttt ttatcaatcg atttatagcc attgtaaaag tttcgcaatg caatttctct
     1321 tttgttagta ccatccgata atatcaattg tgtgttgagt gaaagaaaga aaatggtaaa
     1381 atctcctata ggtgatgcgt tcacaaaatt tccagcaagc gtagcaatat ttcgtatggg
     1441 tgtgctcgaa actaattttg aatactgagc aaaatgaggg aaatattttt caattatttt
     1501 cgattccatt aaatcggtaa cagtgcacga tgcgccaata aagcaattat taccatcgat
     1561 aaaaatgccc ttcaattttt tattatcaaa aaggaaatta atttctgcat ccttcatagt
     1621 gtcatgtttc tgcacatata aatctgttcc tccaccgagg ttggttattg ggttgttggt
     1681 tatttcgtta ttggttactg ggttatttgt tattgggtta tttgctatta agttttgtaa
     1741 tcgttctttg atatttaaaa aatattcggg tagtagttta ttttttactg catattgttc
     1801 ggaggattgt gtgttgcgat ttagtaacaa ctcgttaacc aaaatagcgg cacgctctat
     1861 tgatttgtaa ccggtgcatc ggcaaatatt tccatcgata cttgcaataa catttttttc
     1921 tgttgcttcc ttatcactca aacaaaaacc tgcaagcgac attacaaaac cgggagtaca
     1981 aaaaccacat tgagttgcgc cttcatcgct catggcttgt tgtatcggat taaggccact
     2041 catatttatt ccctcaattg taactatgtg cttgttgttt gcattgccaa ttgccatcag
     2101 gcaagatgtt gccgaccgat aacgcaattc accatcaatc aattcgccca ccaaaactgt
     2161 gcatgctcca caatcgcctt cgcggcaacc tatttttgtg ccttttaaat ttttatggta
     2221 acgaataaaa tcaagcaaca ctaaacccgg aggttcggct gttgcaattt ctttatcgtt
     2281 gagaataaat tttatcaaag caaatttatt ttttgaaagg ttgtaaaatt aatacgatgt
     2341 tttatccact ttggctgccc actcatcgaa aacttttttt gcttcatcac gattcaattg
     2401 ctgcatcaac atttttctat cgcttatagc aagttgaatt tcgttataaa taaatgcatc
     2461 gtcaaattcg atggcagcag catcttcttt ggagcagcca tataccaccc tatctggtcg
     2521 tgcccaataa attgctgcca ggcacatggg gcaaggctcg catgaggtgt acaatgtgca
     2581 gcctgtcaat tgaaaggtat ttaaattttt acatgcatcg cgaattgcta ccacttcggc
     2641 atgggctgta gggtcgttgg tagatgttac tttattattg ccgcgtccca caattattcc
     2701 atccttcact atcacagcac caaatggtcc accttcattg ttacgcattc cgtttgctgc
     2761 catttctatt gcctggccca aaaataattt atcgctgtcg tgttctatca ttattatttt
     2821 attatcgtgt atgtaaactc gtgcttacaa aaatagaaaa tattaggtag caaacatttg
     2881 taaacttaaa gatgcttttg gcttagtata ggcgcacatt atatttttgt gccatttttt
     2941 ctatttgctc ttgtgtcaca ttatgtatgt tgtgatcatg atgatacttt tctacagtaa
     3001 ccacaaacaa tttatactta tgcgcggctg ctaattttat ataaggcaaa atttcccaat
     3061 cgaaagtaaa tgtgttgtgc acaaatattt tcggtgactt attctccatt tgtttatctg
     3121 tttgctcttc gcattgtttg tatgcaaaat gatttttatc gtattcgaaa ttgtattgtc
     3181 ccgttgcatt gtcggtaaaa aaatcgtcaa cgctcaggca tggatattta ccatcttcgc
     3241 tgagtatgcc agccaatgtt gactttccac ttccgggcaa acctcgcaaa agtatcaaac
     3301 acaggtcgaa cacattttca ttctgttgta tcatggcaaa aattatgttt ggtaataaaa
     3361 attaaattcc gaaaagttgt cgggagtttt cggttgtgat ttgcgctact tcttccacgc
     3421 ttatattttt tatttcggca atgcgttgta caaccatcac tatatacgat ggttcgttgc
     3481 gcttgcctct gtatggtgtg ggtgcaaggt atgggccatc ggtttcgagt atcacatgtt
     3541 tcaaatcgat ggtttgcata tcaacatcaa gtccggcttt tttaaacgta accacgccac
     3601 caatgccaag ataaaaaccc atatcgataa tttgctgtgc ctgcgaaggt tgaccgctgt
     3661 aacaatgaaa cacaccacgc aagtttaagt gatggtgttg ttgcaatata gcaattattt
     3721 catcggtagc atttcgcgag tgtatggcaa taggaagatt caattccgat gcccaatgaa
     3781 tttgcttaat aaatgcgact tcctgttctt ttacataagt tgtatcccag taaaaatcta
     3841 atcctatttc gccaatggca taaatttctt tccggtgagc atcaatcaac ttttccattt
     3901 gagtcaattg atcggtacaa ttggcagtaa tgtaacatgg atgcaaaccg agcatagcga
     3961 aacaattttg aggatacttg tgtgtaaggg caaacatggg gtccaccgaa tcgaaatcga
     4021 tgttaggtaa aaaaaagcgc tgtactcctt tatccatagc ggtttttaca agttcatcgc
     4081 ggtcggtatc aaattctttc gaatataaat gtatatgtgt atcggtaatt atcatgtggc
     4141 aaaaatactt tttatacttt tgaaaacgca aatcgaaaat cagtgcaaaa aacaaaaatc
     4201 cttattctgc gatttagttc cattggcgac attgtgctca ccacacctat tatacgttgc
     4261 ttaaagcagc aggtgcctga ttgcgaattg cattatttta ccaagaaaca atttcacacc
     4321 gttattcagc acaatcctta catcgataaa attcatttgc ttgatgataa catgagtgaa
     4381 ttgataacag aattgaaaaa tgaaaaattc gattatgtta ttgaccttca caaaaatcaa
     4441 cgcacgtggt tgttgaaatt tcgattggga gtaaaatcat ttacatttga taaaataaac
     4501 cgcgaaaaat ttttaatcac aaaacttaaa attgatgtac tacccaaaga acatattgta
     4561 gacaggtatt ttgaagcggt tcaaaaaatc aatgtaaaaa atgatgagca gggtctcgac
     4621 tattttgtag ctgagaacga ctatgttgat ttgaaaaaat atgatatcga gaacggaaca
     4681 aaatttattg ccattgctat tggtgcacaa catgccacta agcgattgcc aacaaataag
     4741 ctcatcgaaa tttgtaaaaa aataaacagt caggttattc tacttggcgg cccaacggat
     4801 gtaacaacag gaatagaaat tgcaaatgcc tgcaaaagca atgtcattaa tttgtgtggc
     4861 gagcttacat taaaccaatc ggcatttgta ataaagcatg cttacaaaat aataacacac
     4921 gataccggac tgatgcacat tgccgcagcc atgaaaaaac ccattgtgtc tgtttgggga
     4981 aacaccattc cgcagtttgg tatgtatcct tattatggaa atgaaattat tgaaagtaaa
     5041 attgtggaag ttaaaaattt atcgtgccgg ccatgcagca aattaggtta taacaaatgc
     5101 ccgaaatatc acttcgattg catgaataaa attgatgtga atgaggtggt attattttca
     5161 aatgattgaa tccaaaaacg atctacaaat tcttgtaaat tttcttctga actaagaggg
     5221 tcagggattg tttcgcagtt cgagaatccc ttcgactgcg ctcagggtga caagactcac
//
LOCUS       DAORQA010000118         5182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4028_length_5182_cov_24.843573, whole genome shotgun sequence.
ACCESSION   DAORQA010000118 DAORQA010000000
VERSION     DAORQA010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4028_length_5182_cov_24.843573"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PK736_06585"
     CDS             <1..356
                     /locus_tag="PK736_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HRC33093.1"
                     /translation="RGAFIDQSQSMNIFIQDVNFAKLTSMHFYAWEKGLKTGMYYLRT
                     KSASDAIKFTVEKKYMDEATDNNTEAAFKAISEKETVAANAVYSTENIVAEDDAISQL
                     SCSLDNPDDCAACGS"
     gene            681..1673
                     /locus_tag="PK736_06590"
     CDS             681..1673
                     /locus_tag="PK736_06590"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRC33094.1"
                     /translation="MNKNVAVIGGGSWATANVKMLANNCDHIRWWVRNNETVDFIKKY
                     KHNPNYLSDVEFNLDKIFVSNDVALVVEGCDTVIMNVPSAFLTASLLPLTKKHLQGKL
                     VFSAIKGIVPDRNMIVGEYLHKQYDIPYSHIGVITGPCHAEEVAMEKLSYLTVASLNR
                     EHAAHLAQLVSCRYIRTTLSDDIYGTEYSAVLKNIYSIAAGICHGLGYGDNFQAVLIS
                     NASREIKRFIDTVHPILRDINDSAYLGDLLVTAYSQFSRNRMFGAMLGKGYPVKFAQL
                     EMNMIAEGYYAVKCIHEINKEYKVDMPICDAVYNVCYENITPRVEMRLLTDKLN"
     gene            1750..1824
                     /locus_tag="PK736_06595"
     tRNA            1750..1824
                     /locus_tag="PK736_06595"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1783..1785,aa:Arg,seq:cct)
     gene            2203..3624
                     /locus_tag="PK736_06600"
     CDS             2203..3624
                     /locus_tag="PK736_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC33095.1"
                     /translation="MEEIIGRSDEITIFEERYNSSKSEFIAVYGRRRVGKTFLIRSVF
                     KQRFTFQMSGIANATLQQQLDNFQKTIVEQYPRIKTKPVTNWLDAFEIIKTVINKSKQ
                     KKKVLFIDELPWLDTSRANFIQALEHFWNSWASARKDVLLIVCGSAASWMINNLINNR
                     GGLHNRVTQRMKIEPFTLHECELFVQAKKMALDRYNIIQLYMVMGGIPFYWDEVRKGY
                     SAVQNIEQLCFAKNSVLRMEFPNLLKSLFDKAEKHEAVVLALAKKSKGLMRDEIIRET
                     KLTNGGSLTRLLNELEESGFITKYTPFGKQARNSLYQLSDFYTLFYLKFIKTHKLADK
                     NFWTKTIDNPKHRAWSGYAFEQVCLYHVNQIKRALGISGIHTETSSWRSTTTNSGAQI
                     DLVIDRRDQAINLCEIKFSVKPFVIDKKYDAELRNKIGTFRDETKTRKAVFLTMITTF
                     GLHKNMYAHNVQNDLQMEMLFKK"
     gene            complement(3666..>5182)
                     /locus_tag="PK736_06605"
     CDS             complement(3666..>5182)
                     /locus_tag="PK736_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRC33096.1"
                     /translation="VKYILNRMEDMRMTGKRHPYSSDFKIGLNKELRIIAYEVVFYQN
                     AGAAADLSPAVMERTLFHCTNSYFIPNVNATAYSCRTNLPPNTAFRGFGGPQGMFAIE
                     CAIAKAAAELKIDASEIQKINLIKTGDEFVYGQIAASEANECWQKAEQLYDVEKLQAE
                     VKKFNSENALIKKGLAIQPICFGISFTKTLMNNARSLVHVYSDGSVNISTGAVEMGQG
                     VNTKILQVAQSIFSIPAEHIKVNSTNTFRIANTSPSAASATADLNGKATFLACKAIAE
                     RLKIVAAEELKSDPEKIELKNEFVFADGKKSEITWKQLVSMAFVKRVNLSEHAHHATP
                     DIHFDATKEKGHPFAYHVYGTAILTATVDCLRGTYEFDSVKVVHDFGQSMNEIIDRGQ
                     CEGGIVQGIGWMTMEEIIYGKEGKLKSNALSTYKVPDVYSAPKEIAIHFLETKNNSLA
                     IFNSKAVGEPPLMYGIGAYFALRNAVLAYNPNSVIPYDAPMTPEKVLLSLHQGK"
BASE COUNT         1658 a          929 c          987 g         1608 t
ORIGIN      
        1 accgtggcgc atttatagac caaagccaaa gcatgaatat ttttattcag gatgtaaatt
       61 ttgcaaaact cacgagcatg catttttatg cctgggaaaa aggattgaaa accggcatgt
      121 attatttgcg caccaagtcg gcaagcgatg caattaagtt tacagtagag aaaaaatata
      181 tggacgaggc tacggataat aataccgagg cggcatttaa agctataagt gaaaaagaaa
      241 cggtagccgc taatgcagta tattcaacag aaaatattgt tgcagaagat gatgccataa
      301 gccaactaag ttgttcactc gataatcccg atgattgcgc tgcatgtggc agttaaattg
      361 aaatgtcttt agaaattaat acgttaaaat ccggctcttc atttggccgg attttttatt
      421 ttgtggctta ggtgttttgg gataacacat taaaaaatga aaattaaaaa taaaatatgc
      481 cgaataagat gcacagaaac tattgctttg tattacaaat ttacccttaa cgttcttgtg
      541 taggtcgaat tttttaaaaa actcaagtta ttgtaagaac cataaaaaca ttttttctat
      601 tgttgctgcc atttatttgg ttttcaaaaa atagaaaagg ctatctttac aaaaagtaat
      661 aaaacggcaa gcacaataca atgaacaaaa atgtcgcggt aataggcgga ggaagttggg
      721 caacagcaaa tgtaaaaatg ctggccaata actgtgacca tatacgatgg tgggtgcgca
      781 ataatgaaac cgtagatttt ataaagaaat acaaacacaa cccaaactac cttagcgatg
      841 tagagtttaa cctcgataaa atatttgtat cgaatgatgt agccctggtt gtggaaggtt
      901 gcgataccgt gataatgaat gtgccatctg cattcttaac ggcttcttta ttgccgctta
      961 ctaaaaagca cctacagggt aaattggttt tttcggccat caaaggaata gtccccgacc
     1021 gaaatatgat agtgggcgag tacctccata agcaatatga tattccatat tcgcatattg
     1081 gtgttatcac cgggccttgt catgccgagg aagttgccat ggaaaagctt tcgtatctca
     1141 ccgttgcaag tttgaatcgc gaacatgcgg cacatttagc tcaacttgta agttgcagat
     1201 atatccgtac cacgctgagc gatgatattt atggtacaga gtattcagct gtgctcaaaa
     1261 atatttattc tattgctgcc ggcatttgtc acggattggg ttatggcgat aattttcagg
     1321 cagtacttat ttcaaatgcc tcgcgcgaaa taaaaaggtt tatcgatacg gtgcatccaa
     1381 ttttgcgcga tataaacgat agtgcttacc tgggcgattt gttggtaaca gcttattcgc
     1441 agtttagccg caatcgtatg tttggtgcta tgcttggcaa aggctatccc gtaaagtttg
     1501 cccaactgga aatgaatatg attgccgaag gttattatgc tgtaaaatgc atccacgaaa
     1561 taaataaaga gtataaagtt gacatgccca tttgcgatgc agtgtataac gtatgctacg
     1621 aaaatattac tccacgcgta gaaatgcgcc tactcaccga taagttgaat tgaaatgttg
     1681 atgagtatta cttctatatt tcgtcaaata gaattttagt ttttatacat ttgcattccg
     1741 aactcaaatg gtcccgtagt tcaacggata gaatagaagt ttcctaaact tttgatggca
     1801 gttcgattct gcccgggact accaaaatga gcataccaaa attataatca tagacaatgg
     1861 tgtggttcaa taccaacagc aattaaagca agagaattta ctgtcccttt atttcaaaaa
     1921 gtaaagtgaa atctaaatct ttttcaaatc actactgtca attattgcgt ttttatattt
     1981 ggctctaact agtactttta taccacttac agccaaatat aaaatcgaag gctctaaata
     2041 aatagtattg aaatcgctct tgtttaaatt ttggcattcg tttcattcta tttaaaagta
     2101 taatcaggaa ggtaatcatt tatatttatc aaaaccttca aatttatatt tggctttaac
     2161 tcatgtaaat tgtatactta cagccaaata taaaaataca gaatggaaga aataattggc
     2221 cgttcagatg aaataactat ttttgaggag cgctacaact caagcaaatc ggagtttatt
     2281 gcagtatacg gaaggcgcag agtaggcaaa acatttttaa tacgttcggt atttaagcaa
     2341 cggtttactt ttcaaatgtc aggaatagcc aacgctacat tgcaacaaca attagataat
     2401 tttcaaaaaa caattgttga gcaataccca cgaataaaga ctaagcccgt tacaaattgg
     2461 ctcgatgctt tcgaaataat aaaaacggtg attaataaat cgaagcaaaa gaaaaaagtt
     2521 ttattcattg atgaacttcc ctggttagat acatcgagag caaattttat tcaggcgtta
     2581 gaacattttt ggaattcctg ggcatcggcc cgaaaggatg ttttacttat tgtgtgtggt
     2641 tcggctgcat cgtggatgat aaataactta ataaataatc ggggtggttt gcataatcgt
     2701 gttacacaac gcatgaaaat tgagccgttt accttacacg aatgtgagtt gtttgtacaa
     2761 gcgaaaaaga tggcgttaga tagatacaac attatacaat tgtatatggt tatgggcggt
     2821 attcctttct attgggacga agtacgaaaa ggatatagtg cggtgcaaaa tatagaacaa
     2881 ttgtgctttg cgaaaaatag cgtgctgcgt atggagtttc caaatttatt gaaatcattg
     2941 tttgacaaag ccgaaaaaca tgaagcagta gtattagcct tggcgaaaaa aagtaaaggc
     3001 ctaatgcgcg atgaaataat tcgcgaaaca aaattgacca atggaggtag tttaactcgg
     3061 ttgttgaatg agttggaaga aagcggtttt attaccaagt ataccccctt tggcaaacaa
     3121 gcacgaaaca gtttatatca actttccgat ttttacactt tgttttattt aaagtttatt
     3181 aaaacacata aactagccga caaaaatttt tggacaaaaa caatagacaa cccaaagcac
     3241 cgtgcctgga gtggttacgc ttttgaacaa gtttgtttgt atcatgtgaa tcaaataaag
     3301 cgtgccttag gcataagtgg aattcatacc gaaacatcat catggcgaag cacaacaact
     3361 aatagtggag cacaaataga tttggtaata gaccgtagag atcaagcgat aaacttatgc
     3421 gaaataaaat tttcggtaaa gccatttgtt atcgataaaa agtatgatgc tgaattgcga
     3481 aataaaatcg gcacatttcg cgatgaaaca aaaacacgaa aagctgtgtt tcttaccatg
     3541 attactactt ttggtttaca taaaaacatg tatgctcata atgtgcaaaa cgatttgcaa
     3601 atggaaatgc tttttaaaaa atagcatgga ttgcttttct cgaattggaa aatttgcaaa
     3661 tcattttatt ttccctgatg caaactcaac aatacttttt ctggagtcat aggtgcatca
     3721 tacggaatta cagaattggg attgtacgca agcaccgcat ttcgcaaagc gaagtatgca
     3781 ccaatgccat acattagcgg aggttcacca actgcctttg aattaaaaat agcaaggctg
     3841 ttgtttttag tttcgagaaa atgaattgca atttcttttg gcgcagaata aacatcgggc
     3901 actttatatg tactaagagc attggatttt aattttcctt ccttaccata aattatttct
     3961 tccattgtca tccaacctat gccttgcaca attcctcctt cgcactgccc tctgtcaatt
     4021 atttcattca tgctttgccc aaaatcgtga accactttta ccgaatcgaa ttcatatgtg
     4081 cccctcaaac aatcaacagt agcagtaaga attgctgtgc cgtatacatg atatgcaaat
     4141 ggatgtccct tttctttcgt tgcatcgaaa tgaatatctg gtgttgcatg gtgtgcgtgt
     4201 tccgataaat ttacacgttt tacaaatgcc atcgaaacta attgcttcca ggtgatttcg
     4261 cttttttttc catcagcaaa aacaaattca tttttcaact ctattttttc gggatcggat
     4321 tttaattctt cagcagccac aatttttaat cgctcagcaa ttgctttaca ggcaagaaat
     4381 gttgctttac cgttgaggtc ggcagtggca cttgcggccg atggcgatgt gttggcaatg
     4441 cgaaaggtgt ttgtagaatt tacttttatg tgttcggcag gtatcgaaaa aatgctttgt
     4501 gctacctgta gtatttttgt atttacgcct tgccccatct caactgcacc ggtgcttatg
     4561 tttacactac catcactgta tacgtgcacg agtgatcggg cattattcat tagcgttttt
     4621 gtaaacgaaa ttccaaaaca aattggctga atggctaatc cctttttaat caaagcgttt
     4681 tccgaattga atttctttac ttcggcttgt aatttttcga catcataaag ttgctccgct
     4741 ttttgccagc attcatttgc ttcgctcgct gcaatttgtc catacacaaa ctcatcacct
     4801 gttttaatta aatttatttt ttgaatttcc gatgcatcaa tttttaattc tgcagcagct
     4861 tttgcaatgg cacattctat tgcaaacatg ccctgtggcc cgccaaatcc tctaaatgct
     4921 gtatttggcg gcaagttggt tcggcaacta tatgcagtag catttacatt gggaataaag
     4981 tacgaattgg tgcaatgaaa caatgtgcgt tccatcactg cgggcgaaag atcggcagca
     5041 gctccggcat tttgataaaa cacaacttcg tacgcaataa ttcttaattc tttatttaaa
     5101 ccaattttaa aatcggatga gtaaggatgt cgctttccgg tcatgcgcat atcttccata
     5161 cgattcaaaa tatatttcac cg
//
LOCUS       DAORQA010000119         5097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4099_length_5097_cov_5.429988, whole genome shotgun sequence.
ACCESSION   DAORQA010000119 DAORQA010000000
VERSION     DAORQA010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4099_length_5097_cov_5.429988"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..678)
                     /locus_tag="PK736_06610"
     CDS             complement(154..678)
                     /locus_tag="PK736_06610"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRC33097.1"
                     /translation="MKHSVKFGIIGGLSAVVITVLFYVTGLNLSSMWGAAISGLLMMV
                     VFGVVAVMSVKEERAANGGAITLKSAFVNGFVCLFVGFFISSIYSYIHYNFVEPEFFD
                     KLAEMLATKLAEYQVPADKIEQQVNEILKKKESISTFDYKSALGGLAFAAIYSLIVAA
                     ILKKEKMDDYAPIS"
     gene            1002..1973
                     /locus_tag="PK736_06615"
     CDS             1002..1973
                     /locus_tag="PK736_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRC33098.1"
                     /translation="MDTSQQIDSTRLDLVALIKIFRKWKKHLLITGIVAIVLGFVVTM
                     PFIMPPQFESTVILYPSNLIPYSTESPTETMLQLLESDDIKDRLINTFDLYNHYRIDT
                     TAKFPRTKMYNLLNDQIFITRTAFESVEISVLDEDPKVAAHMCDTIVSYLDWKARSLQ
                     RLKTKEVLTIFENQTKSKKLEIDSLELRLMDIRANYGILDFKTQSKEISKAMYKGASV
                     NKNVADDFNNLKLKGVEAEALDSKLKAARLLYNEYKTSFEKAKSDFAKELTYSNYVTK
                     PLPAENKAKPKRTVFMISLLLAVLFLAFLVILVIENYNTNIKPRLNN"
     gene            1985..3424
                     /locus_tag="PK736_06620"
     CDS             1985..3424
                     /locus_tag="PK736_06620"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRC33099.1"
                     /translation="MITTLSRNIYYSIVATFMALSAVAIALGYWYFLLLPAVVLIAVL
                     GIFRLDILFYLSVAIIPLSINLSETDFGFGFSMPSEPIIFGLMLLIVIKLLLHNTMDK
                     RFIKHPVSIIIIFYLGWMFLTSATSHLPIVSFKYTLARFTFIMVFFYFASELFFKLKN
                     ITKFVAAYLILFCVVIIYTTLNHAAAHFTEKAAHNSMTPFYNDHTAYAVLVAMFIPVC
                     IVMIIGKGFSIISRQWSFVALVLLITALLLSYTRAAWVGIAAAIACSLIFIFRISTQL
                     ISISAIVAALFLLVNVNTIILNLETNREQSSTDVGAHLQSITNIKTDPSNVERINRWS
                     CAWRMFLKRPITGWGPGTYQFVYGPFQLQKEKTVISTLAGNRGNAHSEYLGPLSEQGV
                     PGTLLFVLLALATLITASRVFIYGNNGNVKIIAAGLILGLITYWVHGFLNNFLDTEKA
                     AVPFFGFIAAIVSLDVFHTKPKNEVVPQL"
     gene            complement(3415..4344)
                     /locus_tag="PK736_06625"
     CDS             complement(3415..4344)
                     /locus_tag="PK736_06625"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRC33100.1"
                     /translation="MKLKSLFTVADAATLLNITYAGNGEQTIEGINEIHCVEKGDLTF
                     VDHPKYYDKALQSAATIILINKEVEVPAGKSILISPVPFDDYNKLTRKFMPFRASSKM
                     ISDSAVIGSDTFIFPSAFVGNNVTIGSGCTIHPGVCLYDNSVIGNNVTIHANTVIGAD
                     AFYFKRKPEGYDKMYSCGRVVLGNNVEIGAMCTIDKGVSADTIIGDGTKMDDHVHIGH
                     DTVVGNNCLFAAFAAVAGVTKIEDNVIIWGQVGIQKDIVIGKGTEILAQSGVGKSTAP
                     GSKVFGSPAVDAREKMKELVMIKRLPDLFKNQS"
     gene            complement(4407..4697)
                     /locus_tag="PK736_06630"
     CDS             complement(4407..4697)
                     /locus_tag="PK736_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010181585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRC33101.1"
                     /translation="MKLYTLSAKPYQEEDELLLEDLLEQNHIVLYNDDVNTFDWVIEC
                     LVQICKHDLVQAEQCAHIIHFNGKCSIKEGSYTQLEPICTALLDRGLSAVIE"
     gene            complement(4811..>5097)
                     /locus_tag="PK736_06635"
     CDS             complement(4811..>5097)
                     /locus_tag="PK736_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33102.1"
                     /translation="KYQVTYTIEKKDHVMLFDKTGKLVSYNKQVDKSELPSECETYIN
                     EKFEKPKIVDVINMNINDSIVYSVAMRCKKGDYELFFDKDGKFLKKKSQN"
BASE COUNT         1549 a          934 c          906 g         1708 t
ORIGIN      
        1 ttatttagaa tgaggggaaa aatttcatcc ggcaaatgtg taaacttttt gaaggatttg
       61 aacaagcaca ggtttaagtt ttcaattgct atttgttgaa agtgtaagtt taaacataaa
      121 aggcatttga gaaaattaca cccttcaaat caattaactt atgggagcgt aatcgtccat
      181 tttttctttt ttcaaaatgg cagcaactat gagtgagtat atagcagcaa acgctaaacc
      241 cccaagagca cttttataat cgaaagtact gattgattct ttctttttta aaatttcgtt
      301 tacctgttgt tcaattttat cggccggaac ctgatattcg gcaagttttg ttgccaacat
      361 ctcagcaagc ttgtcgaaaa attcgggctc tacaaaattg taatgaatgt aggaataaat
      421 gctgctgata aaaaaaccaa caaaaaggca aacaaagcca tttacaaaag ctgatttcaa
      481 agtgatggca ccgccatttg cagcacgttc ttctttcacc gacattacag caaccacacc
      541 aaaaacaacc atcatgagca accccgaaat tgctgcaccc cacatgctcg ataaattgag
      601 acctgttaca taaaataaaa cagtgataac aacggcacta aggccaccta tgattccaaa
      661 ctttactgag tgcttcattt atatttttat taagtggtta ttgacgcaaa tgtaaaataa
      721 atttgacttt ataagatgag gattaaaatt tctaattata gagtcaaagt gtacaaacta
      781 tttgttagtg atgggctttg ttgaaaatca ggttagaata tttaaagtgc aattaaaaaa
      841 agtttttgct tgccatggtt gaaaatatag agcaccaaag tagtgaaagt ataaggtttg
      901 aggagcatta aggtcatggt tcaaaacaat aaaggagaaa acatttcgtt ttattaataa
      961 ttttaccttt gcccccctca ttttttaaaa taatatattt gatggatact tcgcaacaga
     1021 tagattcaac acggttggac cttgtagcat taataaaaat atttcgcaaa tggaaaaagc
     1081 atttgcttat cactggcata gttgccattg tgttagggtt tgtggttaca atgcctttta
     1141 ttatgccgcc acaattcgaa tcaacagtga tactttatcc cagtaacctt ataccttatt
     1201 caaccgaaag tcctactgaa actatgttgc agttactgga gtccgatgat ataaaagaca
     1261 ggctcattaa tactttcgat ttgtacaatc attatcgaat agatacaaca gcaaagtttc
     1321 cccgcacaaa aatgtataac ctgcttaacg atcaaatttt tattacacgt acggccttcg
     1381 agtcggtaga gatttcggtg cttgacgaag atcccaaagt tgcagcacac atgtgcgata
     1441 ctattgtatc ctacctcgat tggaaagcaa gatcgttgca acgattgaaa acaaaagagg
     1501 tattaaccat tttcgaaaat caaacaaagt cgaaaaaact cgaaatagat tctctcgaat
     1561 taagattgat ggatatacgt gccaattacg gtatacttga ttttaaaacg caatcgaaag
     1621 aaatttccaa agccatgtat aaaggtgcaa gtgtaaataa aaatgtagcc gatgatttta
     1681 ataatttaaa attgaagggg gtagaggcag aggcacttga tagcaaatta aaagctgccc
     1741 ggttattata caatgaatac aaaacttctt tcgaaaaagc caagagcgat tttgcaaaag
     1801 aacttaccta ttctaattat gtaacaaaac ctttgcctgc tgagaataag gcaaaaccaa
     1861 aacgcactgt atttatgatt tcgcttttat tggcagtttt gtttttagct tttttggtaa
     1921 ttctggttat tgaaaactac aacaccaaca taaagccccg cttgaataat taaacaattt
     1981 gtaattgata accactttaa gccgtaatat ttattattct atagttgcca catttatggc
     2041 acttagtgct gttgccattg cgcttggtta ttggtacttc ctgttgttgc cggcagttgt
     2101 tctcattgcg gtgcttggta tttttcggtt agatattttg ttctatttaa gtgtggccat
     2161 tattccactt tccataaacc tgagtgaaac cgattttgga tttgggttta gtatgcccag
     2221 cgaacctatt atttttggat tgatgttatt gatagtgata aagcttttgt tgcacaatac
     2281 catggataaa aggtttatta agcatccggt gagtatcatc attatttttt acctggggtg
     2341 gatgttttta acttcggcta ccagccattt gcctatagta tctttcaagt atactctcgc
     2401 gcgatttaca tttatcatgg tcttctttta tttcgcatcg gagttgtttt tcaaattaaa
     2461 aaacatcaca aaatttgtag ccgcatattt aatattgttt tgtgtggtga ttatttacac
     2521 tacgttgaat cacgctgcgg cacattttac cgaaaaggcc gcacataatt ctatgacacc
     2581 tttttacaac gaccacacag cttatgccgt tttggtagca atgtttattc ctgtttgtat
     2641 tgttatgatt attggaaaag ggttttcaat tatttcgcga caatggagtt ttgtagcatt
     2701 ggtgctttta ataaccgcat tgttactttc atatacccgt gccgcctggg ttggtattgc
     2761 tgccgcaata gcttgtagtt tgatttttat ttttcgcata agcacacaac ttatttctat
     2821 ttctgctatt gtagccgcat tatttttact ggtgaatgta aataccatta tcctcaatct
     2881 cgaaacaaac agagagcaat catcaaccga tgttggggct catttgcaat ctattacaaa
     2941 tataaaaaca gacccttcga atgtggaacg cataaaccga tggagttgtg catggcgtat
     3001 gtttcttaag cggccaatta ccggatgggg ccccggcacc tatcaatttg tttacggccc
     3061 gtttcaactt caaaaagaaa aaactgtaat aagtacactt gcgggtaaca ggggaaatgc
     3121 gcacagcgaa tatcttgggc cactatcgga acaaggtgta ccgggtactt tgttgtttgt
     3181 gttgctcgcg cttgcaactt tgattacagc atctcgtgtt tttatttacg gcaacaatgg
     3241 taatgtaaaa attattgctg ccggccttat attggggtta ataacttatt gggtgcatgg
     3301 ttttttaaat aattttcttg atactgaaaa agcggcagta ccattttttg gtttcattgc
     3361 cgccattgtt tcactcgatg tttttcatac caaaccaaag aatgaggttg ttcctcagct
     3421 ttgattttta aataaatcgg gcaggcgttt tatcattaca agttctttca ttttttcgcg
     3481 tgcatcaact gcggggctgc caaatacttt actgcccgga gcggtgcttt ttcctactcc
     3541 cgactgtgct aatatctccg tgcctttgcc aatcactata tctttttgaa tgccaacctg
     3601 tccccaaata ataacattgt cttctatttt tgtaacaccg gcaactgccg caaaagctgc
     3661 aaataaacaa ttgttaccaa caacggtgtc atgacctata tgaacgtggt cgtccatttt
     3721 tgttccatca ccaataattg tatcggcact tactccttta tcaatggtgc acattgcccc
     3781 aatttcaaca ttattgccta atactactcg accacaacta tacattttat cgtagccttc
     3841 gggtttgcgc ttaaaataaa aagcatcggc accaataacg gtatttgcat gaatggtaac
     3901 attatttcca ataacgctgt tatcgtataa acatactccg ggatgaatgg tgcaaccact
     3961 tcctatggta acattattgc caacaaaagc actgggaaaa atgaaagtat cactgcctat
     4021 cactgccgaa tcgcttatca tttttgatga agcacggaaa ggcataaatt tccgggtaag
     4081 tttattatag tcatcaaaag gaacagggct tatgagtatt gattttcctg caggtacttc
     4141 tacctcctta tttatcaaaa taattgtagc ggccgattgt aatgctttgt cataatattt
     4201 gggatgatcg acaaaagtaa ggtcgccctt ttctacgcaa tgaatttcgt ttatgccttc
     4261 tatagtttgc tcgccattac ctgcataggt aatgtttaat aatgttgcag catcggccac
     4321 agtaaagagc gattttaatt tcatgtttgt ttaaataaaa tgtgttttgt tttttgaaat
     4381 ggttggaaag tattgtaacg ttttacctac tctattacag cagataatcc acggtcgagt
     4441 agggcggtgc aaatgggttc gagttgagta taactgcctt ctttaatcga acactttcca
     4501 ttgaaatgta tgatatgcgc acattgttcg gcttgtacca gatcgtgttt acaaatttgc
     4561 acaagacact caataaccca atcgaaagta tttacatcat cgttgtataa aacaatatga
     4621 ttttgttcga gcaggtcttc gagtagaagt tcgtcttctt cctgataagg tttcgcagat
     4681 aatgtataca gtttcatttt aaatgccttt ttgcaaatat agtatttcta taaattacat
     4741 aacaaaatat aagttctaat agcttaatat ttcaaaaaaa aatgagtaat atttttgctg
     4801 atgagcttaa ttagttctgg cttttctttt taagaaactt cccgtctttg tcaaaaaaca
     4861 gctcatagtc ccctttttta caacgcatag ccactgaata aactatcgag tcgtttatgt
     4921 tcatattgat aacatcaaca attttgggct tttcaaattt ttcgttgata tatgtttcgc
     4981 attcagaagg caattcagat ttatcaactt gcttattgta acttaccaac ttacctgttt
     5041 tatcaaataa cataacatgg tctttctttt cgatggtata tgttacctga tatttgc
//
LOCUS       DAORQA010000120         5082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4109_length_5082_cov_16.451164, whole genome shotgun sequence.
ACCESSION   DAORQA010000120 DAORQA010000000
VERSION     DAORQA010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4109_length_5082_cov_16.451164"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PK736_06640"
     CDS             <1..602
                     /locus_tag="PK736_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33103.1"
                     /translation="LYISKSNNLYTKKSALIKYTLVFLCICFCVNFSFGQISADYVRW
                     SATKKLTIDDFSIKTKANESTIAFAQFYMEYQLKGFDFLTKNFNKKVHNYMIKSASWI
                     DTTANINQAIIYEQTLFDLSEIYARTFRKELKENRKKLVNGLQIAEELNAKIMADFSK
                     RRIEYDLETKFGTDVEQQKAWEKQIQKELLLLEEFAYDK"
     gene            complement(745..1572)
                     /locus_tag="PK736_06645"
     CDS             complement(745..1572)
                     /locus_tag="PK736_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33104.1"
                     /translation="MSVTRTRIILLVIAAILLTYLIYRLPIANTNAKKSDSIDKVEEV
                     AIPLDWEKFIDSASSGLLPEQISQLRAVTEKPNFEKDIVAIDTVAMMWEQFNYPGIAA
                     HYKEKIAQQQPNEKNCLAAAFRYFDAFKIATDETERKAMVDAAILNYKKVIEYNPENL
                     NAKTDLGVLYAEGTSDPMAGIMLLREVVAKDPKHENAQMNLGLLSLKSNQNDKAIERF
                     ENVLQINPARLEMYVYKAYAYVAMKDTSKAKENFELFLKNSDDEVAKQQVKDYMKEL"
     gene            complement(1631..2224)
                     /locus_tag="PK736_06650"
     CDS             complement(1631..2224)
                     /locus_tag="PK736_06650"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRC33105.1"
                     /translation="MFTGIIETIGHVKSIIKDESNLHFWIESNITRELKVDQSVAHNG
                     VCLTVVEIKDDSYRVTAIDETLQRTNLGTLQAGGKINIERCMVLNGRLDGHIVQGHVD
                     CTATVTHIDNANGSWVYRFSHVTQALPLTVEKGSITVNGVSLTVVDAGDDFFSVAIIP
                     YTYEHTNFHQFVTGTIVNIEFDVLGKYVANMLKHLKN"
     gene            complement(2254..4875)
                     /locus_tag="PK736_06655"
     CDS             complement(2254..4875)
                     /locus_tag="PK736_06655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRC33106.1"
                     /translation="MKHRFLLILVVGLMGFILFCNQRANATHNRAGEITYRQLGLYFY
                     EVTITVFTQPSSNIDRPTLELFWGDGAKDSLPRISNDPVPGVYDVARITYVGTHNYPG
                     PATYTLHFEDQNRNANVQNIPNSVNAAFYVQSQLTINPFLGANSSPQLLNYPIDMGAV
                     GAIFIHNAGAVDPDGDSLSYELFICKCGGGINCPGYTFPDATNSFSIDAITGDLVWDT
                     PKFIGLYNAAFLIKEWRKGINIGYVERDMQIEIININNDPPVIVDVPDTCVEAGSFLS
                     FNVTANDINNDPVTLTATGGAFALSPDSAYFAQPASGTGTVTAAFSWQTTCMHVRKNP
                     WTISFKAKDTHVNNLPPPPSISFVDFETVNITVVAPAPKNLTVVPIGNSMTVTWDKEF
                     CDSAKGYYVYRRNGFYGYVHGYCETGVPAYTGYVKVATLQGINTLTFKDNNNGAGLIP
                     GIDYCYMIVAWFADGAESYASDEVCAQLKHDLPVITNVSIDTTNNINGKCYVAWSKPI
                     DLDSTITPGPYIYYLYRATGFTGQNAVLVDSLFSLNDTTYTDTVINTRDNPLSYRIDF
                     YNNTPGQYFKVGSTQVASSVFLTPSPTDKAVVLTWTEQVPWQNGAYTIFRRNILNTWD
                     SINTVNAKIYKDTGLVNGNQYCYRVQSIGGYSAGGYVFPIVNYSQEVCAVPIDNEPPC
                     APVMKLQSLCELQRNVITWQYDFINCPNDVQQFNIYYATTLTADPQLIAVITNVSDTI
                     FVHDTLASVAGCYFMTATDFIGNESAKSDSICVDNCPLYELPNVFTPNGDGINDLFHP
                     FPYSYVEKIRIVIYDRWGREIFTTENPDINWDGNNQQSNKPCPDGVYYYICTVYELYL
                     DGLKPRTLTGFVQLLRN"
BASE COUNT         1566 a          971 c          948 g         1597 t
ORIGIN      
        1 ttttatatat ttcaaaaagt aataacttgt acacaaaaaa atcggctcta attaaatata
       61 cattagtttt tttatgcatt tgtttttgcg taaacttttc ctttggacaa attagtgccg
      121 attatgttcg atggagtgcg acaaaaaaac ttacaataga tgacttttca ataaagacta
      181 aagcaaatga atcaaccatt gctttcgctc aattttatat ggaatatcaa ttgaaaggat
      241 ttgacttctt gacaaagaat tttaataaga aagttcataa ttacatgatc aaatctgctt
      301 cttggattga cacaacagct aatattaatc aagcaattat ttatgaacaa accctatttg
      361 acttaagcga aatttatgca agaacatttc gaaaagaatt aaaagaaaac agaaagaagc
      421 tggtaaatgg tttgcagatt gcagaagaat taaatgctaa aattatggct gatttttcaa
      481 aaagacggat tgaatatgat ttggaaacaa aatttggaac agatgtggaa caacaaaaag
      541 catgggaaaa gcagattcag aaggagctat tattgcttga agaatttgca tacgataaat
      601 aattttctcg aataaccggt gttgtgtcaa ttaaatttta gagctattca ttcgtagcaa
      661 taaaattaaa acagatagaa atataggtca ttagatggag ttaaaatctt ttgtgttttt
      721 gggttccaaa aaattttaaa aaaactacaa ctccttcata taatctttca cttgttgctt
      781 ggcgacttca tcgtcagagt tttttaaaaa caattcgaaa ttttctttgg ctttactggt
      841 atctttcatt gcaacatatg cataagcttt gtaaacgtac atttcaagac gagcaggatt
      901 tatttgcaat acattctcga accgttcaat ggctttatca ttttgattcg actttaatga
      961 aagcaaacca agattcattt gtgcattttc gtgtttagga tctttggcaa ccacttcgcg
     1021 cagcaacatt atgcctgcca taggatcact tgtaccttcg gcatacaaca ctcctaaatc
     1081 ggtcttagca tttaaatttt cagggttgta ttcaatcact tttttataat ttaaaattgc
     1141 agcatccacc attgctttgc gttcggtttc atcggtagca attttaaatg catcgaaata
     1201 gcggaatgct gccgctaaac aatttttttc gtttggttgt tgctgtgcaa ttttctcttt
     1261 gtagtgtgct gctatgccgg gataattaaa ctgttcccac atcattgcca ctgtatcaat
     1321 tgccacaata tccttttcga agtttggctt ttcggtcaca gcacgtagtt gcgaaatttg
     1381 ctccggcaat aatccgcttg aggcactatc aataaatttt tcccaatcga gtggtatggc
     1441 aacttcttcc actttatcaa tcgaatccga tttttttgca ttcgtatttg caattggaag
     1501 tctgtaaata agataggtta aaagtatggc cgctattact aacaggatta tgcgtgtgcg
     1561 tgttactgac atgtgtggtg agaattattt ttgcgcgaat ttattaatta aaaaacattg
     1621 gtggattaaa ttaatttttc aaatgcttca acatgttcgc tacatatttt ccaagcacat
     1681 caaattcgat atttacaatt gtgccggtta caaactgatg aaaattagtg tgctcgtatg
     1741 tgtaaggaat tatggcaaca gaaaaaaaat cgtcaccggc atcaaccact gtaaggctta
     1801 caccgtttac cgttatagat cctttctcca cagtgagtgg tagtgcttgt gtaacatggc
     1861 taaatctgta aacccagctg ccgttagcat tgtcgatgtg tgtaacggtt gccgtgcaat
     1921 cgacatggcc ttgaactatg tgaccatcga ggcggccatt taaaaccatg caccgttcta
     1981 tgtttatttt accaccggct tgcagtgtgc caaggtttgt ccgttgcagt gtttcatcaa
     2041 tggctgttac gcgatagcta tcatctttta tttctaccac agtaagacac acaccattat
     2101 gcgccacact ttggtctact ttcaactcac gggttatatt actctcaatc caaaaatgta
     2161 aattactttc gtctttaata attgacttaa cgtggcctat ggtttcaata attcctgtaa
     2221 acataaattt tgattaatgg tgcaaagaaa aaattaattg cgcaacaatt gcacaaagcc
     2281 tgtaagcgtg cgcggtttaa ggccatctaa ataaagttcg taaacagtgc agatgtaata
     2341 atacacccca tcgggacatg gtttatttga ttgttgattg ttgccatccc aattaatatc
     2401 agggttttcg gtagtaaata tttcgcgacc ccagcgatcg taaatcacta tgcgaatttt
     2461 ctctacataa ctatatggaa atggatgaaa caaatcatta atgccatcac catttggagt
     2521 aaatacattg ggcaattcgt acaaggggca attgtcaaca caaatactat cactctttgc
     2581 cgattcgttg ccaataaaat ctgtagcagt cataaagtaa catcccgcaa ccgaagccaa
     2641 tgtatcatgt acgaaaatag tatcgctcac atttgtaatc acagcaatca attgtggatc
     2701 ggcagttaat gtggttgcat agtaaatatt aaattgttgt acatcgtttg ggcagttaat
     2761 aaaatcatat tgccaggtaa ttacattgcg ttgcaattca cacaacgatt gtagtttcat
     2821 tacaggcgca cagggaggtt cgttatctat aggcacagca caaacctctt gcgaataatt
     2881 cactattgga aaaacatagc cgcctgcact ataaccacct atactttgca cacgatagca
     2941 atattgattt ccgtttacaa gcccggtatc tttatatatt tttgcattca cagtgttgat
     3001 agaatcccat gtgttcaaaa tatttctacg gaaaatagta taggcaccat tttgccaggg
     3061 cacttgttcc gtccaggtta acactacggc cttatcggtc ggtgaaggtg taagaaacac
     3121 acttgatgcc acttgtgtgc tgcccacttt aaaatactga ccgggagtat tgttataaaa
     3181 atcgatacga tacgatagtg ggttatcccg ggtgttgata acggtatctg tatacgttgt
     3241 atcattcaaa gaaaataacg aatcgaccaa cacagcattt tgtcccgtaa aacctgtggc
     3301 gcggtacaaa tagtaaatat atggcccggg agtaattgta ctatccaaat cgatgggttt
     3361 actccatgcc acataacact tcccattaat attattagta gtgtcgatac tcacatttgt
     3421 tattacggga aggtcgtgtt ttagttgagc acatacttcg tccgaggcat agctttcggc
     3481 accatcggca aaccatgcca ctatcatata acaataatct ataccgggta taagtccggc
     3541 accgttatta ttatctttaa aggtaagtgt gttaatgcct tgtaaggttg ctactttaac
     3601 atagcctgta tacgctggca cacctgtttc gcaatagcca tgtacataac cgtaaaatcc
     3661 atttctgcgg tatacgtaat aaccttttgc actatcacaa aattctttat cccacgtcac
     3721 ggtcatcgaa tttcctatag gcactactgt taaatttttg ggtgccggtg ccaccacagt
     3781 aatgttcacc gtttcgaaat cgacaaagct tatgcttggt ggcggaggta aattatttac
     3841 atgcgtatcc tttgctttaa aagaaatagt ccatggattt ttgcgtacat gcatgcaagt
     3901 ggtttgccag ctaaacgctg cagtaaccgt tcctgtgccc gatgcaggtt gagcaaagta
     3961 tgccgaatcg ggcgacagcg caaacgcacc acccgtggca gtaagtgtaa cgggatcgtt
     4021 attgatatca tttgctgtaa cattgaaaga taaaaagctg ccggcttcaa cacaggtatc
     4081 gggcacatcc acaatcaccg gaggatcgtt atttatattt ataatttcta tttgcatatc
     4141 gcgctctaca taaccaatgt ttattccctt acgccattct tttattaaaa atgctgcatt
     4201 atataaacca ataaatttgg gcgtatccca caccagatcg cccgtgatgg catcaatgga
     4261 aaaagaattg gtggcatcgg gaaaagtata gcccggacag ttaatacctc caccgcattt
     4321 acaaataaat agttcgtacg aaaggctatc gccatcgggg tcaacggcac cggcattgtg
     4381 aataaatatt gcgccaacag cacccatatc gatgggataa ttgagaagct gtggcgaact
     4441 atttgctccc aaaaatggat tgatagttaa ttgcgattgc acataaaaag cagcattcac
     4501 cgagttggga atattctgta cattggcatt acggttttga tcttcgaagt ggagcgtata
     4561 tgtagcaggg ccgggataat tgtgtgtgcc tacataagta atgcgtgcaa catcataaac
     4621 accaggaaca ggatcgtttg aaatacgtgg taatgaatct ttagccccat cgccccaaaa
     4681 caattcaagt gttggcctgt ctatattgct cgatggctga gtgaaaacag taatggtaac
     4741 ttcgtaaaaa tataaaccca actgcctgta ggtaatttct ccggcacgat tgtgagtagc
     4801 atttgccctt tggttacaga atagtatgaa gcccatcagc ccgacaacca atataagcaa
     4861 aaacctgtgt ttcatgtttg ttaatttcaa tacgctcaat attaatagat gcgaaaatta
     4921 catattattc cgttaaacta ttttattaat caacttatat tgcaattatt gtacaaaggt
     4981 gcagagttat tttgcggcaa catatttgtt atagtactgg tacaaacttt ataaagaatc
     5041 gtttgttagg aaattatccc gtttcggcac aataaatttt tt
//
LOCUS       DAORQA010000121         5082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4111_length_5082_cov_6.288442, whole genome shotgun sequence.
ACCESSION   DAORQA010000121 DAORQA010000000
VERSION     DAORQA010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4111_length_5082_cov_6.288442"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PK736_06660"
     CDS             <1..360
                     /locus_tag="PK736_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRC33107.1"
                     /translation="NPFATYCDSAIMTAFFDSNVVNYMSQYFYAVKMDAESRDSVTFG
                     DHTFTYMPDQKANELAISLLNGKMSYPTFVIMDENIKLLSPVAGYQTAPQMMPILKYF
                     GDNIYLKQNWEEYSKTK"
     gene            436..717
                     /locus_tag="PK736_06665"
     CDS             436..717
                     /locus_tag="PK736_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC33108.1"
                     /translation="MLVLNNITFEFGSRALFKDTSWHIKPGEKIGLIGRNGTGKSTLL
                     RVISKEYSLSGGEITGRKDMSIGFLNQDLLSYESDIGSGHAGIRKRIDT"
     gene            complement(782..1264)
                     /locus_tag="PK736_06670"
     CDS             complement(782..1264)
                     /locus_tag="PK736_06670"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HRC33109.1"
                     /translation="MMRIAVLASLVFIILGGCNAQSTNKGNDSLANGEKRIQWMSFTE
                     AVEKNKTAPRKIFIDVYTEWCGWCKKMDKTTFVDSNVVNYMSQYFYAVKMDAESRDSV
                     TFGDHTFTYMPDQKANELAISLLNGKMSYPTFVIMDENIKLLSPVAGYQTAPQMLPVV
                     "
     gene            1343..1942
                     /locus_tag="PK736_06675"
     CDS             1343..1942
                     /locus_tag="PK736_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33110.1"
                     /translation="MNNKALYIAVFVFSFFAFNCKKEKQSIEESGNINSNNNPFATYC
                     DSAIMTAFFDSTQYFPTAFYNSYLLFNSSNNHGRKLVVKTYFNGGTFTMTITNWEWQN
                     PPNYGVLAKTYSTLATDSNHACMLQNGNNLCDGVEATFRIGNDYLSTAYTNYQGIVTI
                     TECDVIKNTITGTYKVKVQDITGNFPHMIEGTFKNLCYP"
     gene            complement(2122..4254)
                     /gene="feoB"
                     /locus_tag="PK736_06680"
     CDS             complement(2122..4254)
                     /gene="feoB"
                     /locus_tag="PK736_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRC33111.1"
                     /translation="MNNQRIRVALVGNPNCGKSTIFNALTGLNQKIANFPGVTVEKKS
                     GNCTLYSDQGHAVSAEIIDLPGTYSLYPKTLDEQIPFQVLCDSHNESYPDIAVVVADG
                     TNLKRSLFLCSQVIDLKIPVVLVINMMDMVEAAGLKIDIAALEKILEVKVITLNARKG
                     TGIEELKRTICQKHPVRTTDFLDCTTFIPNMCEALRAKFELTCNYAAFLSAHNYKSVS
                     LFKMNAYAKDGLQKILTAHDFDGHRTQAAETLERYKVITEVMEQCVRNPEMEAATSFS
                     SKIDRILTHRIWGFVCFLLIMFFMFQTIFSLAKIPMDFVDYTFSALSQKISQILPGGI
                     FSDLFINGILAGLGGIVIFIPQIALLFAFIAVLEDSGYMARVSFIMDKLMRKFGLNGR
                     SVIPLISGIACAVPAILSTRTIQNWKERIVTIMVTPLMSCSARLPVYSLLIAIIIPSR
                     NIFGFLSLQGIVLMIMYLIGFFAALGAAWLLKYFVKTRERSFFIMEIPVYRMPRWKNV
                     FFTIIDKVKVFLFDAGKIILAISIILWVLSTFGPISRQQSIADKYAVLKTAPNADSSA
                     LYYQFQSERLENSYAGIVGHWIEPAISPLGFDWKIGIALITSFAAREVFVGTMSTIYS
                     VGENGDQLKTVREKMMAEKNKITGKPRYDFATGMSLMLFYAFAMQCMSTLAVVKRETG
                     RWMYPIFQFIYMGALAYLASLAAYQYFK"
     gene            complement(4267..4515)
                     /locus_tag="PK736_06685"
     CDS             complement(4267..4515)
                     /locus_tag="PK736_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRC33112.1"
                     /translation="MVTKVKEKIKLSQMHKGESGIIDSFTNDEMKLKLMEMGCLPGEK
                     ISISSHAPFGCPVAIKLSGYTLSLRMEEAENVIVTRTK"
     gene            complement(4881..>5082)
                     /locus_tag="PK736_06690"
     CDS             complement(4881..>5082)
                     /locus_tag="PK736_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33113.1"
                     /translation="VSIYDGRGSLKFEVRGLKSNAGYFTIDVNCTGGLASGERGARWA
                     NGLYVVHLSTEKERLSKKFIKE"
BASE COUNT         1562 a         1049 c          947 g         1524 t
ORIGIN      
        1 aatccttttg caacatattg cgatagtgct ataatgacgg cctttttcga tagcaatgtg
       61 gtaaattata tgagccaata tttttatgcc gtaaaaatgg atgccgaaag ccgcgattcg
      121 gtaacctttg gcgatcacac atttacttac atgcccgacc aaaaagcaaa tgaactggca
      181 atatcgttac tcaatggcaa aatgagttat cctacttttg taattatgga cgaaaacata
      241 aaactacttt cccctgttgc cggatatcaa acagcgccac aaatgatgcc catattaaaa
      301 tattttggcg acaatattta tctgaaacaa aactgggaag aatattccaa aacaaaataa
      361 ttttaataca acatttatta agcttaagaa tatttttcag cttaattttt tcaaaaacaa
      421 aaatcaaaac gagcaatgct ggtactcaat aatattactt tcgaatttgg gtcgcgcgct
      481 ttattcaaag atacttcgtg gcatattaag ccgggcgaaa aaataggact cataggccgc
      541 aacggaacag gcaagtcaac tttactgcgc gttatttcca aagagtatag tttgagtggt
      601 ggcgaaatta ccggccgcaa agatatgtcg atagggtttc tcaatcagga tttactttca
      661 tacgaatcgg atattggaag tggccatgct ggcattcgaa aaagaattga tacttgaaaa
      721 aaaaatagaa gcattgctgg tagaactgga acacaatcac gatgaaaaat tattgcacaa
      781 cctacacgac tggcagcatt tgtggcgctg tttgatatcc ggcaacaggg gaaagtagtt
      841 ttatgttttc gtccataatt acaaaagtag gataactcat tttgccattg agtaacgata
      901 ttgccagttc atttgctttt tggtcgggca tgtaagtaaa tgtgtgatcg ccaaaggtta
      961 ccgaatcgcg gctttcggca tccattttta cggcataaaa atattggctc atataattta
     1021 ccacattgct atcgacaaat gtagtcttgt ccattttttt gcaccagcca caccattcag
     1081 tatatacatc aataaaaatt ttgcgtggtg ctgttttgtt tttttctact gcttcggtaa
     1141 acgacatcca ctgtatacgt ttttctccat ttgcaagtga atcattacct ttattggtcg
     1201 attgtgcatt acaacctccc aaaattatga aaactaaact tgctaatact gctattcgca
     1261 tcataggttt ttatattttt tgcaaatgta ccatttatag cctattttcg ttagataata
     1321 tttgccactg tctgaaaata gtatgaataa caaagcctta tatattgctg tttttgtttt
     1381 ctcctttttt gctttcaatt gcaagaaaga aaaacagagc attgaggagt cgggtaatat
     1441 taacagcaac aacaatcctt ttgcaacata ttgcgatagt gctataatga cggccttttt
     1501 cgatagcaca caatattttc ctacagcgtt ttataactca tacttactat tcaattcttc
     1561 gaataatcat ggccgaaagt tggtggtgaa aacatatttc aatggtggca catttactat
     1621 gaccataaca aattgggaat ggcaaaatcc cccaaattat ggagttcttg caaaaacata
     1681 cagcacgctt gccacagata gtaaccatgc atgtatgcta caaaatggta acaacttatg
     1741 cgatggagta gaggctacct ttagaattgg caacgattat ctttcaacgg catacactaa
     1801 ttatcagggc atagttacca ttaccgaatg tgatgtaata aaaaatacta tcaccggaac
     1861 ctataaagta aaagtgcaag acatcacagg aaattttcct catatgatag aaggtacgtt
     1921 taaaaatctt tgctatccgt aaactgttta gattcacttt tcactttgtc tcacaacttc
     1981 tgcacttcaa aaaattattc cacaaagttt cacagagata aactcaaaag gttcacaaag
     2041 aattaaagcg tccactgacc tttgtgcaac ttcattcttt ctttgtgcta ctttgtgtca
     2101 caacttcggc acttcaaata attatttgaa atactgatac gctgcaaggc ttgccaaata
     2161 agccaatgcg cccatgtaaa taaattgaaa aatgggatac atccatctgc cggtttcgcg
     2221 ttttactact gccagtgtac tcatacactg catagcaaag gcataaaaaa gcatgagcga
     2281 catacctgtt gcaaaatcat accgtggttt gccggttatt ttattttttt cggccatcat
     2341 tttttcgcgc accgttttta gttgatcacc attttcgcca acactataaa tggtactcat
     2401 agttcccaca aacacttcgc gagctgcaaa agaagtaatg agtgcaatcc caattttcca
     2461 atcgaaacca agagggctta ttgccggttc tatccaatgc ccaacaatgc cggcataaga
     2521 attctctaac ctttcacttt gaaattgata atacaaagcg ctactatcgg cattgggagc
     2581 ggttttcaat acagcatatt tatctgcaat ggattgctga cggctaatag gaccaaaggt
     2641 gctgagcacc caaaggatga tagaaatggc aagaattatt tttccggcat cgaataaaaa
     2701 cacttttact ttatcgataa tggtaaaaaa aacattcttc caacgcggca tgcggtacac
     2761 aggaatttcc ataataaaaa aagaacgctc gcgagttttt acaaaatatt ttaaaagcca
     2821 tgcagcacca agcgcggcaa aaaatcctat aaggtacatt atcataagca caatgccttg
     2881 caggctgagg aacccaaaaa tattgcgcga tggaatgatg atggcaataa gcaacgaata
     2941 cacaggcaaa cgtgccgagc agctcatgag tggtgttacc ataatagtaa ctatgcgctc
     3001 cttccagttt tgaatagtgc gtgtgcttaa tatggcaggt acagcgcagg caatgccgct
     3061 aataagggga ataacactgc gaccgttgag cccgaatttg cgcataagtt tatccataat
     3121 aaaacttaca cgggccatgt atccactgtc ttcgagcacg gcaataaatg cgaacaacaa
     3181 tgctatttga ggaataaaaa taacaatacc accaaggccg gcaagaattc cgtttataaa
     3241 caaatcgcta aaaataccgc ccggcaatat ttgcgatatc ttttggctca atgccgaaaa
     3301 agtataatca acaaaatcca tggggatttt tgccaacgaa aaaattgttt gaaacatgaa
     3361 aaacatgatc aacaaaaagc acacaaaacc ccaaatgcgg tgtgtaagta tgcgatcgat
     3421 ttttgatgaa aacgatgtgg ccgcttccat ctcgggattt cgtacacatt gttccattac
     3481 ttcggtaatt actttatagc gctccaaagt ttcggcagct tgtgtgcggt ggccatcgaa
     3541 atcgtgtgca gtaagaattt tttgcaagcc atctttagca taagcattca ttttgaacaa
     3601 cgacaccgac ttataattgt gggctgaaag aaatgcggca tagttgcatg taagttcgaa
     3661 cttggcgcgc aaagcctcgc acatattggg aataaatgtg gtgcagtcca aaaaatcggt
     3721 agtgcgaaca ggatgtttct ggcaaatggt gcgcttgagt tcttctatac ctgtgccttt
     3781 gcgggcgttg agggttatta cttttacctc taaaattttt tcaagtgcgg caatatcaat
     3841 tttcaatcct gcagcctcta ccatatccat catattgata acaagcacca ccggaatttt
     3901 taagtcaatc acctgcgaac ataaaaataa actgcgttta agattggtgc catcggctac
     3961 caccactgca atatcggggt acgactcgtt gtgcgaatcg cagagtacct gaaacggaat
     4021 ttgttcgtca agtgttttgg gatacaaact gtaagtgccc ggcaggtcga taatctcggc
     4081 cgatactgca tgcccctgat cgctgtataa ggtgcagttg cccgattttt tttctactgt
     4141 aacaccggga aagttggcta ttttttggtt taagcctgta agcgcattaa atattgtact
     4201 ttttccacaa ttgggattgc ctactaacgc tacccgtata cgttggttat tcacttggcg
     4261 gttttatcat ttagtacgcg ttactattac gttttcggct tcttccatgc gaaggctgag
     4321 ggtgtatccc gaaagtttta tggccacagg gcaaccaaat ggtgcatgac tgctgatgct
     4381 tattttttcg cccggcaggc aacccatttc catcaacttc agcttcatct catcgttggt
     4441 gaacgaatcg atgatgcccg attcgccttt atgcatttgc gataacttta tcttctcttt
     4501 tactttcgtt accattttta tccttgtaaa gcaacattgt taatacagga gaatgttgcg
     4561 tgtcgttttt attttacggc acaaatgtaa ttaataatgg atagaaattt taggaggctt
     4621 taatttgaaa gaataaccgg ctttaagtag cggttgcatt gtattttggg aggttgaaat
     4681 caatgcttgt caagtaatgc attattcttc ccgtcattgc gattgcgatt actatcggaa
     4741 gaagcaatct cagtggagta aagacaaagt gtaagaaaga ttgcttcgct aacctcgcaa
     4801 tgacgtgtta tcctctgcta ccttaagtac cttagcttgt tgcgttcgta aacttttatt
     4861 cgtattttag aaatattcaa ctattcttta ataaacttct tactcagccg ctccttctcc
     4921 gtactcaaat gtacaacata caacccattt gcccaccgcg cacccctctc gcctgaggcg
     4981 agaccacccg tacagtttac gtctatagta aaatatcctg cattgctctt caaacctcga
     5041 acttcaaact tcaaacttcc ccgcccatcg tatatactca cc
//
LOCUS       DAORQA010000122         5069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4130_length_5069_cov_6.092740, whole genome shotgun sequence.
ACCESSION   DAORQA010000122 DAORQA010000000
VERSION     DAORQA010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4130_length_5069_cov_6.092740"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..4492
                     /locus_tag="PK736_06695"
     CDS             32..4492
                     /locus_tag="PK736_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33114.1"
                     /translation="MSSDFTPNPVILGATTSSATPLIQPTANVQDTTLLHSTSNFSFL
                     DSNLCFSCNLSASISSNNGNTLCFSDSVILTSNNVSVNYLWSTGATTKSIVVKTPGTY
                     MLDIAEGACSSTVSFAIQGNPQIISSLTTDSCICNADSTGKLNLTASGGSGGLNYLWN
                     NSVTTMNILQAAAGFYSVTITDVKGCSTTATAIVHQPNQISINPTLSTPTCPFGLNGA
                     MNIIVNGGAAPLTFLWNNGNTTTSLSNIAAGTYTVIVSDSKGCSNASAITLSDPNIIS
                     NQVFLINPNCNSGNNGSIELETIGGTAPYTYLWSNGNTNSIASGLVSGSYVVTITDAN
                     NCTASFSQVITQPSALVINETISQPTCSSLNNGSIDITVNGGGGNYQYLWNDGTTNAV
                     IDSIFIGAYTITVTDSLGCVIQKSFVLNNVDSLLISASIANAGCGISNGAITIAVTQG
                     SGTYSYLWSNGNTTDTINQLQPGVYTVTVTDNGSCSQTATYSIQQNSLFSVQSLVNYV
                     SCFGLSDGSISTQVSGGNPPFTYLWSNGGTTNGITNLNSNSYSVLITDGAGCTTVENF
                     SITEPPALQVSKQVNDVSCSGLADGSVSLFTSGGTTPYNYLWSNGNTNSSVSQLNTGS
                     YSITITDANGCFSNTSITINSPLTLSINITSTVASCNIANGVANCTASNGNGGYLYLW
                     NNGSTISSLTNVFGGIYTVTVADSKGCSISATVNLSNPNTPTLSLNSKTNVTCFGSAT
                     GSIDILKTGGTPPFNYLWNDGSTTQDLNNRNAGFYAVTITDINGCTATYADTIFSGIE
                     VEITPTTSNVLCGGAATGSISINVNGGQPGYTFQWSNNLALNSYINSNLAAGTYTVTV
                     TDGIGCSKTTAFQIDEPNTLGLSPSSTPTVCNGSTGTAFVYVTGGVQPYQYEWSNNTY
                     NNFVQNLSAGVYIVTVTDAYGCSNSTSVQVTTPDGPSILIDTVQAISCNGLIDGKVHL
                     SITNGTAPYTYTWTNGATVQDLNNLASGQYSVVVTDANGCTTIGSFSIVEPDLISIAG
                     TINNVSCNGGNDGAILTNANGGTLPFQYLWSLSNTTSSLINLGIGTYTVTITDNNGCT
                     KKNSFTIIQPTALLLSDSIRHLICANDADGFIFINATGSNGSFSYQWNNGSTDSALQN
                     LSGGNYTVTVTDSLGCYATKQITLNEPLAMTVNSTISDVICNGAATGSVTTAVNGGTG
                     NKTYLWSSGATSNSISNVLAGTYTVTVTDANNCTIQMVNTITQPQAINLTSTVVTNQC
                     FGDSTGSIVVNAAGGASPYDFLWSNGKTTSNIINMPAGTYSLTVTDNSNCTFSASLLL
                     TEPGQLNASVTLNAQQGYAFAIANGGVAPYSYIWQNELDSSIVSTLDTANLTAGTYYL
                     TVIDSNACQIILPVFNINTGFASSSNETQVLLYPNPFTNELYISISKYKSSLKKIEII
                     DMQGRIIYFESIDNKSNFKGIINTLGFSKGIYVLRIHFNTGPEVFKLVKQ"
     gene            complement(4599..>5069)
                     /locus_tag="PK736_06700"
     CDS             complement(4599..>5069)
                     /locus_tag="PK736_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33115.1"
                     /translation="RRDFEIFINRENTNLIYENVFLKLEVWEDFLDCMDKDGLQARYN
                     RAIKNCDIFVSLFFKKVGKFTEQEFEVAFGKFTETSKPKIYTYFKNADVKTGAWKKED
                     FYSLDSFETKLAALGHHKTGYENIQDLKYQFKMQLDKLIPIMSQSKITMDRNQF"
BASE COUNT         1623 a          890 c          922 g         1634 t
ORIGIN      
        1 gataaaacaa agttttgatt ttgaatcttg cttgagttct gattttactc ctaatcctgt
       61 catactcgga gcaacaacat catcggccac tcctttaatt caaccaacag caaatgtaca
      121 agacacaact ttgcttcatt ctacttcaaa tttttcattc ttagattcaa atttatgttt
      181 ttcttgtaat ctgagcgcat caatttcttc aaacaatggt aatacattat gttttagcga
      241 tagtgtgata ttgacttcga ataatgtaag cgttaattat ttgtggagta caggagctac
      301 tacaaagtca attgttgtaa aaactcccgg cacatatatg cttgacattg ctgaaggtgc
      361 gtgtagttct acggtgtctt ttgcaataca aggcaatcca caaattattt caagtttgac
      421 aacagattct tgcatttgca atgcagatag tacagggaag ttaaatctta cagcttcagg
      481 cggaagtggg gggttgaatt atttatggaa taacagtgtg acaactatga atattctaca
      541 agcagcagca gggttttata gcgtaacaat aaccgatgta aaaggttgca gcacaacagc
      601 cacagccatt gttcatcagc caaaccagat cagtattaac cccacattat caacaccaac
      661 ttgtccattt ggtttgaatg gggctatgaa tatcattgta aacggaggtg ctgcaccatt
      721 aactttttta tggaataatg gtaataccac aacgagtttg tcaaatattg ctgccggcac
      781 atatacagtt atagtgtctg atagtaaggg gtgttcaaat gccagtgcaa taactttatc
      841 cgacccaaat ataatttcga atcaagtatt tttgattaac cctaattgca atagtggcaa
      901 taacggcagt atagagttag aaaccattgg aggtacagcg ccatatactt atctatggag
      961 taatggcaat actaattcga ttgcatctgg gctcgttagc ggtagctatg ttgttactat
     1021 tactgatgca aataattgta cggcttcatt tagtcaggta attacacagc ccagcgcctt
     1081 ggtaattaat gaaacaatct cacaacctac atgttcaagt ttaaataacg gcagtataga
     1141 tattactgta aatggtggtg gtggtaatta ccaatattta tggaatgatg gtacaacaaa
     1201 tgctgtgatt gacagcatat ttattggtgc atatactatt accgttacag atagtttggg
     1261 ctgcgtgatt caaaagagtt ttgtacttaa taatgttgat tcacttttaa tttcagcaag
     1321 cattgccaat gccggatgtg gaataagtaa tggggcaatt actattgcgg taacacaagg
     1381 tagcggaacc tatagttatt tgtggagtaa tgggaataca actgatacca tcaatcaact
     1441 acaaccggga gtttatacgg ttactgtaac tgacaatggt tcatgtagcc aaacggcaac
     1501 atattcaatt caacaaaatt ctttgttttc tgtacaaagc ttagttaatt atgtttcttg
     1561 ttttggctta agcgatggtt caatatctac gcaggtttca ggtggaaatc ccccatttac
     1621 ctacctatgg agtaatggtg gcactacaaa cggaatcact aatctcaatt caaatagcta
     1681 ttctgtttta attactgatg gtgcaggatg caccactgtt gaaaatttta gtatcaccga
     1741 accaccggca ttgcaagtaa gcaagcaagt aaatgatgtc tcatgtagtg ggctggcgga
     1801 tggaagtgtt agtttgttta catcaggtgg taccacgcct tacaattatt tatggtcaaa
     1861 tggcaacact aattcatccg tttcacaatt gaacacaggt tcatattcaa ttactataac
     1921 tgacgccaat ggatgttttt caaatacttc tattacaatt aattcacctt taacattgtc
     1981 tattaatata accagcacag ttgctagttg taacattgca aacggagttg ccaattgtac
     2041 agcttcaaat gggaatggag gctatttata tttatggaat aacgggtcaa caatttcttc
     2101 cttaacaaat gtttttggtg gcatatatac cgtgacagtt gcagatagca aaggatgtag
     2161 catttcagct actgtaaatt tatcaaaccc taatactcct acactatcgc ttaattcaaa
     2221 aacaaatgtt acttgctttg gtagtgctac tggctctatt gatatattaa aaactggagg
     2281 tacacctcca ttcaattatt tatggaatga tgggtcaacc actcaagatt taaataacag
     2341 aaatgccgga ttttatgcag taaccataac cgatattaat ggatgcacag caacttatgc
     2401 agatacaatt ttttcaggaa ttgaagttga aattactcct acaacaagca atgtgctttg
     2461 tggaggagcg gctactggca gtataagtat aaacgtaaat ggtggtcaac cgggttatac
     2521 ctttcaatgg tctaataacc ttgcactgaa ctcttacatc aactcgaact tagctgccgg
     2581 aacttatacg gttacagtca cagatggcat aggatgcagc aaaacaacgg catttcaaat
     2641 tgatgagcct aatacattgg gattaagtcc aagtagtacc ccaacagtat gcaatggctc
     2701 tacaggcaca gcgtttgttt atgtaacagg aggagtgcag ccttatcaat atgaatggag
     2761 taataacaca tataataatt ttgtacaaaa tcttagtgca ggcgtttata ttgttacagt
     2821 tacagatgct tatggatgtt ctaattcaac aagcgtgcaa gtaacaaccc ctgacggtcc
     2881 ttcaatcctt attgatacgg tgcaagccat aagctgcaat ggccttattg atggcaaggt
     2941 acacttaagt attacaaatg gcacagcacc ttatacttac acttggacaa acggtgcgac
     3001 tgtgcaagat ttaaataatt tggcaagtgg acaatattca gttgtagtaa ccgatgcaaa
     3061 tggatgcaca actatcgggt cgtttagcat agttgaacca gatttaattt ctatagcagg
     3121 tactataaat aatgttagct gtaatggcgg taatgatggt gctattttga caaatgccaa
     3181 tggcggtaca ttgccatttc aatatctatg gtcgttaagc aatactacat cttcactaat
     3241 aaatttaggc ataggaactt ataccgtaac tattacagac aataatggtt gtaccaaaaa
     3301 aaatagcttt accattattc aaccgacagc gttgctcctt tcagattcaa ttaggcattt
     3361 aatatgtgcg aatgatgcag atggtttcat atttataaat gctaccggaa gcaatggaag
     3421 tttttcatat caatggaata atggcagtac tgatagtgct ttgcaaaatt tgagtggtgg
     3481 taattataca gtaactgtta cagattcatt gggctgttat gctaccaagc agattacttt
     3541 aaatgaacct ttggcaatga ctgttaattc aacaatttca gatgttatat gtaatggtgc
     3601 cgcaacgggc tcagtgacta ctgcagtgaa tggtggcaca ggtaataaga cttatctgtg
     3661 gagttcaggt gcaacaagta attcgataag caatgtattg gctggaacgt atactgtcac
     3721 agttactgat gcaaacaatt gcacaattca gatggtaaat actattactc aaccacaagc
     3781 tattaatttg acaagtactg ttgttacaaa tcagtgcttt ggagatagca caggaagtat
     3841 tgtcgtcaat gctgctggag gagcttctcc atatgacttc ctatggagca atggaaaaac
     3901 gaccagcaat attattaata tgccggcagg tacttattct ttaacagtga ccgataatag
     3961 taattgtact ttctcggctt cgttgctttt gaccgagcca ggtcaactga atgcaagtgt
     4021 tactttgaat gcgcagcaag gttatgcttt tgcaattgca aatggaggcg ttgctccata
     4081 ttcatacatt tggcaaaatg aattagattc ttcaattgta tctacactcg acactgcaaa
     4141 tttaacagca ggtacatatt atcttacagt tattgatagc aatgcttgcc aaatcatatt
     4201 accagtattc aatattaata caggctttgc ttctagttca aatgaaactc aagttctcct
     4261 ttatccaaat ccttttacaa atgagttata tatttcaatt tcaaaatata aatctagtct
     4321 aaagaaaatt gaaataattg atatgcaagg ccggataata tattttgaga gcattgataa
     4381 taagtcaaac tttaaaggta taatcaatac tttaggattt agcaaaggaa tttatgtttt
     4441 aagaatacat tttaatacag gacctgaagt attcaagtta gtaaagcaat aaattcttca
     4501 gctaactttg cttctttgtc taactgcaat ttgaattgat aatgtgaatc ctcaccaatt
     4561 tgcaatctcg attttatttg ttctatggtt agcataattt agaattgatt tctgtccatt
     4621 gtaattttcg actgagacat aattgggatt aatttatcca attgcatttt gaattgatat
     4681 ttcaaatctt ggatgttttc atatccagtt ttgtgatgac ccaaggcagc taatttagtt
     4741 tcaaatgaat ctaaagagta aaaatcttct tttttccaag cccccgtttt aacatcagca
     4801 tttttaaaat acgtataaat ttttggctta ctggtttctg taaatttacc aaatgccacc
     4861 tcaaattctt gttcggtgaa cttacctact tttttaaaaa ataaagaaac aaaaatatca
     4921 caatttttaa ttgcgcgatt ataccttgct tgcaaaccat ctttatccat acaatcgaga
     4981 aagtcctccc acacttctaa ttttaaaaat acattttcgt aaattaaatt tgtattttct
     5041 ctattaataa atatttcaaa atcccttct
//
LOCUS       DAORQA010000123         5048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4149_length_5048_cov_10.428600, whole genome shotgun sequence.
ACCESSION   DAORQA010000123 DAORQA010000000
VERSION     DAORQA010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4149_length_5048_cov_10.428600"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PK736_06705"
     CDS             <1..636
                     /locus_tag="PK736_06705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33116.1"
                     /translation="GNTNLLKQSYVNPLPGDVNNELASQLVALALNIRFDLLDPNFSP
                     NSTVSFKDMLVDSGPMAGMTVMEVYNEGNAALSGCASKFTRALLRTFIAHINSSWSLG
                     KQRNNVLTCPKDPCGKTWKDVTADGDFLQFGAYPNPSAGVLNIEFIDNNTHTTSIQLY
                     DLTGKLVYTQEGQSRTGYNKVTLNLENLPKGVYMLRATVNLDTRNVRVILQ"
     gene            774..1322
                     /locus_tag="PK736_06710"
     CDS             774..1322
                     /locus_tag="PK736_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC33117.1"
                     /translation="MNDFTNIATLISALKNKQHNAYEYLYDNYSEALYGVIHKVLNDD
                     EHANDILQDTIVTIWQKIDSYNPEKGTLFTWMLNIARNKAIDEFRKNKRNPKISIDEL
                     SVHTFDMQNVNNLEVNATDVIGLKKIVNGLKPDLRQMIDMHYFNGMTHQEITDATQMP
                     LGTVKTKIRTAMMQLKSIFEIK"
     gene            1319..2119
                     /locus_tag="PK736_06715"
     CDS             1319..2119
                     /locus_tag="PK736_06715"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HRC33118.1"
                     /translation="MNIDEYIASGILEQYVTGSASPQEMREVECMSHIYPEINAEVSR
                     LQLAIEQYAKSHSKRPPAHLRNTILAKLKTLEDETPKPVVEPVNNVIEFNKPKTNTSI
                     WKYSAAASIILLAVSSAYFINNQNQKNKEIASINEALEERNNQIENLSGDLAFYSNPD
                     YKKIVLAGVKEKSPESVVTIYWNKNKAEVYLVVNNLPTAIANKQYQLWAIKDGVPVDM
                     GMLPTDVAQNSLIKMKQIDNAQAFAITLENTGGVPSPTMSEMYVIGAL"
     gene            complement(2677..3762)
                     /locus_tag="PK736_06720"
     CDS             complement(2677..3762)
                     /locus_tag="PK736_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33119.1"
                     /translation="MKNIIYKNKICDVSNCTKYICAIICFMFCGIVAQSQTVAKAEYR
                     FDDDKGFGKNTQVSLILAADSTWVLPTISTSGLAVGYHVLYIRTRDSNKKWSQTTRIN
                     IEIVANETQTKDSIIAAEYFIDFEPSGGYGFATDIPLSQKDSSITKSFTIPTSVFASL
                     PIGYHTIYGRVKNKLGLWSETFRNNIELIKSEDARSVVKVEYFFSGNDDIGFGACYSK
                     TISIKPPFTVDKDTFYIPLNQLTYGNNITLYIRAKESVTGIWSQTAKKGGLNVVPPGP
                     RLATTTEENSYSMYPNPVSDHLNIDLANFNQETMQFILTNLVGEVMQSQEIKNQSTQI
                     KINQPPGVYFVTLNSSTQTITKKIVIQ"
     gene            complement(3797..4942)
                     /locus_tag="PK736_06725"
     CDS             complement(3797..4942)
                     /locus_tag="PK736_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33120.1"
                     /translation="MKKINLFTTVAAILIALNFMSVQNANARIWRINVLSNYQANGPW
                     GDNFGGNASNPVFKNIGDAHSNTNLVQNGDTLYLEGCPQGVSFPGVTITRPLVIIGTG
                     YYLTINANTSNDLLQSRLVYIYFNAGSAGSQIIGVELTGSYNTQVNTDNITIKRCYIN
                     SSTNIFVLNNVKDININQNFFDNTNSNTNSGITFSSYNAPGAGLTIRNNIFKRTLLVR
                     SGNTIYDVAKCDNNIFDCPYLAGGSVIMKCASFKNNIIKTPAITLNINEGISSSKVAY
                     NTIATQTQLDTAAGYNNKYLSVANMSNLFVTPTASIDGNYRTKGNIYDYKGNDNAPRG
                     AFGGASVTARYTLSGNAAIPIVYDISTTGVATQANGLQVIIKARSIK"
BASE COUNT         1635 a          872 c          875 g         1666 t
ORIGIN      
        1 ggtaatacca atttattgaa acaatcatat gtgaatccat tgcctggcga tgtaaacaat
       61 gagcttgctt cacaacttgt agcacttgca ttgaacattc gtttcgactt gcttgatcca
      121 aacttctcgc caaacagcac agtgtcgttc aaggacatgc ttgttgattc aggaccaatg
      181 gcaggcatga cagtaatgga agtatacaat gaaggtaacg ctgccttatc aggatgtgcc
      241 agtaaattca cccgcgcact tttacgtacg ttcattgcac atatcaatag ctcttggtcg
      301 cttggcaaac aacgcaataa tgtgttgaca tgtccaaaag acccatgtgg aaaaacgtgg
      361 aaggatgtta cggccgatgg tgatttctta caatttggcg cttatccaaa tccaagtgca
      421 ggtgtgttga atatagagtt catagataac aatactcata ctacgagtat acaactttat
      481 gacctcaccg gtaaattagt atacacacaa gaaggccaaa gcagaacagg ctacaacaag
      541 gttacattaa atcttgaaaa tctgcctaaa ggggtttaca tgcttagagc cacagttaac
      601 ttagacacac gtaatgtgcg tgttatttta caatgatcaa ttagcccctg atcacttttt
      661 tgaacgcccc tgtcgaaaga caggggtttt ttttgtcttt ttcttgattg aatattatag
      721 attatttttt tccatatcaa tgccaccaaa tatttttata cttttgaaaa cttgtgaatg
      781 actttaccaa tatagcaacg ttaatttctg ctttgaaaaa taagcagcac aatgcttatg
      841 aatatttgta cgacaattat tccgaagctt tgtatggtgt tatacataaa gtattgaatg
      901 acgatgaaca cgcaaatgat atactacaag atactattgt aaccatttgg caaaaaatag
      961 attcttacaa tcccgaaaaa ggaacattgt ttacatggat gctaaacatt gcacgaaata
     1021 aagccataga tgaatttcgg aagaataagc gcaatccaaa aatctcaatt gatgaattaa
     1081 gcgtacatac atttgatatg caaaatgtaa ataatctgga agtgaatgca accgatgtaa
     1141 ttgggctgaa aaaaatagtg aatggtttaa aacccgactt gaggcaaatg atagacatgc
     1201 attattttaa tggcatgaca catcaggaaa taacagacgc aactcaaatg ccactgggca
     1261 cagtgaaaac aaaaatacga acggcaatga tgcaactaaa atctattttc gaaataaaat
     1321 gaatattgac gaatacatag cttcgggtat actggaacaa tatgttaccg gttctgcttc
     1381 gccacaagaa atgcgcgagg tggaatgtat gagtcatatt tatcctgaaa taaatgctga
     1441 ggtaagtaga ttgcaactcg ctattgaaca atatgccaaa agtcacagta aacgccctcc
     1501 tgcacattta agaaatacaa ttttagcgaa attgaaaacg cttgaagatg aaactccaaa
     1561 accggttgtg gaacctgtaa ataatgtaat agagtttaat aagcccaaaa caaatacttc
     1621 tatttggaaa tacagtgctg cagcatcaat catattactt gcagtatcat cagcttattt
     1681 tataaataat cagaaccaga aaaacaaaga gattgcttcc ataaatgaag cacttgaaga
     1741 gcgtaacaat cagatagaaa atttgtcggg cgatttagct ttttattcta atcccgatta
     1801 taaaaaaatt gtgcttgcag gtgtaaaaga aaaaagtccc gaatcggtgg ttaccattta
     1861 ctggaataaa aataaagcag aggtttattt ggtagtaaac aatttgccaa ctgccattgc
     1921 caataaacaa tatcagttgt gggcaattaa agatggtgtg cctgttgata tgggaatgct
     1981 gccaacggat gttgcacaaa attctttaat aaaaatgaaa caaatagata atgcccaggc
     2041 atttgctatt acactcgaaa ataccggtgg tgtgccaagc cccactatga gcgaaatgta
     2101 tgtgataggt gcattgtagt tttatattga ttgattagca ctcaacatca tttgcctttt
     2161 agattttgtt tttatttatc ttgataaggc agtataaaaa tcttgttctt tttctgaaat
     2221 attgctgtta gtaccctctc tttgaaatta ttaaagagta tactgctatc taaatttaac
     2281 caaagttacc atataggatt atattttgtt gccacaaatt catttgcgat cctgctaaca
     2341 tatgagaaaa cttttaccgc atcttttgct tccgcaacaa aactaaaata tatatacttc
     2401 gcataaatca aagtgcaaga ttggggagga aaattatatt gcctagtttc tttgattctg
     2461 taatggtgta agtaattttt tttgctttgt aaaggttatg tcaagaattc gaaacaatat
     2521 cgagaattaa tattaaataa gtataccctg aataatttag ctgacacaac actttttcat
     2581 aataggtagc tatttgcaat aaaaactttc cgaaagtttt ttcttccgga aagttttgaa
     2641 atattatgtt accactgcaa atatttttgc aacagattat tgtatcacaa ttttctttgt
     2701 aattgtttgt gtgctgctat tcaatgttac aaaatatact cctggtggtt ggttaatttt
     2761 tatttgtgtc gattggtttt ttatttcctg tgattgcatc acttcgccaa ctaaatttgt
     2821 aagtataaat tgcatagtct cttgattgaa attagccaaa tcaatattca aatggtcgct
     2881 cacaggattt ggatacatac tataagaatt ttcttctgtt gttgttgcaa gccttggtcc
     2941 tggtggtaca acattcagtc cacccttttt tgccgtttgc gaccatatcc ctgttacgct
     3001 ttcttttgct cgaatataca aagtgatatt attgccataa gtaagttgat tgaggggtat
     3061 gtaaaaagta tctttatcta ctgtgaatgg tggttttatc gaaatggttt ttgaatagca
     3121 agcaccaaaa cctatatcat catttcccga aaaaaagtat tccactttta caactgatct
     3181 ggcatcttcg cttttaatca actcgatatt gtttcgaaat gtttcgctcc acaggccaag
     3241 tttattttta actctgccat aaatagtgtg gtagccaatt ggcaaactcg caaaaacaga
     3301 agtgggaata gtaaacgatt tggtgatact tgaatctttt tgcgaaagtg gaatatcggt
     3361 tgcaaaacca taaccaccgc ttggttcaaa atcaataaaa tattccgcag ctataatgct
     3421 atcttttgtt tgcgtttcgt ttgctactat ttcgatattt attcgtgtgg tttggctcca
     3481 ctttttgtta ctatctctgg tgcgaatata caatacatga taaccaacgg ctaaccccga
     3541 agtagaaatg gttggtaaaa cccaagtact atctgcagca aggattaaag agacttgggt
     3601 gttttttcca aaccctttat catcatcaaa acgatattct gcttttgcaa ctgtttgcga
     3661 ttgtgctact attccgcaaa acataaaaca aataattgca cagatatatt tagtacagtt
     3721 tgatacatcg catattttat ttttataaat aatatttttc atctttttag ttttttaaat
     3781 tgagtgtgat gaaaatttat ttaatactgc gtgccttaat tattacttgc aaaccattgg
     3841 cttgtgtggc aacacctgta gttgatatgt catacacaat tggtatggca gcattgcccg
     3901 aaagtgtata gcgtgcagta acagaagcac caccaaaagc accgcgtgga gcattatcgt
     3961 ttcctttata gtcataaata tttccttttg ttctataatt tccatcgata gatgcagtag
     4021 gcgttacaaa caaattgctc atgttggcca cgcttaaata tttgttgtta tatcctgccg
     4081 cagtatccaa ttgcgtttgt gttgcaatag tattatatgc aacttttgac gaggaaatac
     4141 cctcattaat attcaaagta attgcaggag tttttataat attattttta aaactagcgc
     4201 atttcattat tacagaacca ccggcaagat atgggcaatc gaaaatatta ttatcacatt
     4261 ttgccacatc ataaatagtg ttgccagacc tcacaagtaa ggtgcgttta aaaatattgt
     4321 tgcgtattgt tagcccagca ccaggtgcat tataactact aaatgtaatt cctgaatttg
     4381 tgttgctatt tgtattgtca aaaaaattct gattgatgtt tatatcttta acattattca
     4441 aaacaaaaat atttgtcgag gaattgatat aacatctttt aattgtgata ttgtcggtat
     4501 taacttgtgt gttatagctg ccggttaatt caacaccaat gatttgtgaa ccagccgaac
     4561 cagcattgaa gtaaatatat actaatctag attgtaacaa atcgtttgat gtatttgcat
     4621 tgatggttaa ataataaccg gtgccaataa taaccaaagg ccgagtgatt gtaactccag
     4681 gaaaactcac tccctgcggg caaccttcta aataaagcgt atcaccattt tgcacaagat
     4741 tggtgttgct gtgcgcatca ccaatatttt taaaaaccgg attgcttgca ttgccgccaa
     4801 aattatcgcc ccaaggccca tttgcttgat agttggagag tacgttaatt ctccatatgc
     4861 gcgcattagc attttgaacg ctcataaaat ttagtgcaat taatattgca gctactgtag
     4921 taaataaatt gatttttttc atcatctttt tttttaattg tttaactttt ttaaattatt
     4981 tatttgtttt tattacggtg caaacatata ttgaatagca cccacaaact atctcctttt
     5041 attattcg
//
LOCUS       DAORQA010000124         5044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4154_length_5044_cov_7.921628, whole genome shotgun sequence.
ACCESSION   DAORQA010000124 DAORQA010000000
VERSION     DAORQA010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4154_length_5044_cov_7.921628"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /gene="tpiA"
                     /locus_tag="PK736_06730"
     CDS             <1..677
                     /gene="tpiA"
                     /locus_tag="PK736_06730"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRC33121.1"
                     /translation="LKSIDLGGKQVILATPYLYLSTVADIIKSNKNIFVAAQDCSEHL
                     SGAYTGEVSASMLQSVGITHVIIGHSERRTYHQEKDEALKTKVENALTNNLKVIFCCG
                     EQLSHREQMKHFNLIEGQLWHGLFHLDETQIINCAIAYEPVWAIGTGVTASPAQAAEM
                     HSFIRKLLIEKYGESTANEISILYGGSCNEKNAAELFSNTDIDGGLIGGASLKADSFI
                     KIIQSL"
     gene            688..1632
                     /locus_tag="PK736_06735"
     CDS             688..1632
                     /locus_tag="PK736_06735"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HRC33122.1"
                     /translation="MKYFVLGVFFLSVLFAHGQSKVEYTSVEIFHGDMVANYPTFPAT
                     DEPIFILRTRMGKNLSGSEIWHRKYGYPKTGINIVVGSLGNASVLGHFGAAMFDFILS
                     QRLSSKLFFEEGASLGAAYFTKKYDEQTNPKNIAIGSHVTAFVNASFNLVYKLNDKND
                     LLLGANIFHGSNSHTVIPNLGVNMPGVSIGYRYNFRSAARVTNADSILRYSRKPKLNV
                     RFALGINEQGNSVNAVNGPKYPIYLSGIFISKKFSPINRVGSGIEVWYNAGVESYIQS
                     HQSDFSDSRKAAWACQYTLSHEFLFSHFSLLTQGGIYL"
     gene            complement(2236..2739)
                     /locus_tag="PK736_06740"
     CDS             complement(2236..2739)
                     /locus_tag="PK736_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33123.1"
                     /translation="MAFMFVVQYLDAQSTTNKKPVKHYEWGNLGVGFTMERLGSFGNG
                     RNYTFDYTRAVGKMYLQGGVQSFVIYQSEFSLYSFHIAAGYAVRNRLFLYGMFIGPAY
                     QSGFLPNGKFFSALGVSANMPFIFKPLEDLGIGIAVYSNINKKVTDNGMRVMINISSK
                     YDRKIVR"
     gene            complement(2816..4339)
                     /locus_tag="PK736_06745"
     CDS             complement(2816..4339)
                     /locus_tag="PK736_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33124.1"
                     /translation="MKSILNKYVHHLFFALKGFTVLAFALGMIFFNSCKKEKLITDAS
                     AKLDFSADTIVFDTVFVTFGSTNQQLLVYNNNSQPINISSIRLSNTNNSYFRLNVDGV
                     SGTKFSDIEIGAKDSMFIFVEVTIDPSSANLPFIVADSIIFETNGNIQDVDLVAWGQN
                     AHFIRADLNIPGLPPLSIIPCVNNLAEWDNVLPYVIFGYAVVDSLCVLKISKGTKIYF
                     YNGSGLWVYRGGCIKIMGTKDEPVKFGGTRLESYYADKPGQWDRIWINDGAFGNEINY
                     AEIKNGFIGLQIETLFDNNSDTSNDKSVMVNNTTISNMSGAGVFARYHNFVMNNCVIS
                     NCASYCAALSLGGTYEFNHCTFANYWKYGQRTTPSIYLNNYTLDNNDNIISFDMHKAL
                     FRNCIIYGNNENEIEVEMKPGGDDFYFFDHDIIKVKSDFSITDPNHFKMIYKNSDPDF
                     KNTNDGDFSLDTMAFAKNKADSTCCSPLHPILQYDLKTTDRNMFLPADIGAIERKDQ"
     gene            complement(4508..>5044)
                     /locus_tag="PK736_06750"
     CDS             complement(4508..>5044)
                     /locus_tag="PK736_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRC33125.1"
                     /translation="EYALSHLAKQFFERTSQRKYIALVWGDFDNAEGTVTGNVGRNIA
                     NRKMMDVFPNGEYGKHAVTHYKVLEKFLYTTLIECKLETGRTHQIRVHMKYIKHPLFN
                     DFEYGGDAILRGTAFTRYKQFVQNCFALLPGQALHAATLGFVHPVTKKEMFFESPLPE
                     NFNTLIEKWRGYSRTYEE"
BASE COUNT         1612 a          873 c          939 g         1620 t
ORIGIN      
        1 cccttaaatc tattgacctt ggcggcaaac aagttattct cgccactcca tatttatatt
       61 taagtactgt tgcagatatt attaaaagca ataaaaatat ttttgtagca gcacaggatt
      121 gtagtgaaca tttatcggga gcatatacag gcgaggtaag tgcatcaatg ttacagagcg
      181 ttggcataac tcatgttatc attggtcatt cggaacgaag aacgtatcat caggaaaagg
      241 atgaagcttt gaaaacgaaa gttgaaaatg cactgaccaa taatttgaaa gtaatatttt
      301 gttgtggtga acaattaagt caccgcgaac aaatgaaaca tttcaatctt atagaaggac
      361 aactttggca tggacttttt catttagatg aaactcaaat tataaattgt gctattgcat
      421 acgagcctgt atgggcaata ggtacaggtg ttacagcctc acccgcacaa gctgcagaga
      481 tgcatagctt catacgcaaa ttgcttattg aaaagtatgg agaaagcaca gccaacgaaa
      541 tttcaatttt atacggtggc agttgcaatg aaaaaaatgc tgcagaatta ttttccaaca
      601 ctgatattga tggcggcttg attggtggtg catcactcaa ggccgacagt tttataaaaa
      661 taattcaatc attataaata tttcgttatg aaatattttg tgcttggtgt tttttttctt
      721 tctgttttat ttgctcatgg gcaaagtaaa gtggagtaca catcagtcga aatatttcat
      781 ggcgatatgg ttgctaacta tcctactttc cctgcaacag atgaacccat ttttattttg
      841 cgaacccgga tgggaaaaaa cctttcgggt agcgaaatat ggcatcgcaa gtatggctat
      901 cctaaaacag gaattaatat tgttgttggt tcattaggca acgcatcagt acttgggcat
      961 tttggtgcgg ccatgttcga ttttatttta tcgcaacggc tgtcgtcaaa attatttttc
     1021 gaagaaggtg cttctttggg cgcggcctat tttacaaaga agtatgatga acaaaccaat
     1081 ccaaaaaata ttgcaatagg ctcgcatgtt actgcatttg taaatgcatc gtttaattta
     1141 gtttataagt taaatgacaa aaacgatttg ctgttaggtg caaatatttt tcatggcagc
     1201 aattcgcata ctgtaattcc taaccttggt gtaaatatgc cgggtgtatc gataggttat
     1261 cgttataatt ttcgcagtgc tgcacgggtt acaaatgctg attctatttt gcgctattca
     1321 cgtaagccaa aattaaatgt gcgttttgca cttggtataa atgaacaggg caatagcgtg
     1381 aatgccgtga acggtcccaa gtatcccatt tatctttcgg gaatatttat cagcaaaaaa
     1441 ttctcaccaa taaatcgtgt tggcagtgga atagaagtgt ggtataatgc cggtgttgaa
     1501 agttatattc aaagccatca atccgatttc agtgattcgc gaaaggcggc atgggcttgt
     1561 cagtatacat tgagtcacga gtttttattt tcacattttt cgttgctcac gcagggaggt
     1621 atttatttat aaaatccatt ttataaaagt gagtataaga atttttagaa cggaaataat
     1681 aagtatttta ttaaaactat tgtcactgca aagttaggtg tgcaatatta ttttaagaat
     1741 gtaataaaga ataatgatgc catgttattt attggcactt atgtaaaaac aaattttggg
     1801 caggccgatt tttgggaaaa tggaattggc ttccagtttt agttttggaa atccttctgc
     1861 atcagtttca acaattttaa gatactatac atattttttt ttagtcccgt agggacgaca
     1921 ttttggtaat aacgaaaacc tatcttcact gaagtcccgt aggggctaca tttttgttgc
     1981 catcaaattt cttgattaaa atttcgcccc tatggggctt tcttttttgg tttacttctt
     2041 tctaccaaaa taccgtccct acaggactat cacggtttca gaataaagtt tgcaaagtag
     2101 tttatcaaaa ttagtatgtt agtagagaat ggcaaagcag tgtgaatgta ttagttagta
     2161 acaatcgtat attcgattaa attcgtaatt agttgcagag aaaatctatt gattaaattt
     2221 atgtagtttt gaaagttagc gtacaatttt tctatcgtac ttgctcgaaa tattaatcat
     2281 tacacgcata ccattgtctg ttactttttt atttatgttt gaataaacgg caatgccaat
     2341 accgaggtct tcgagcggtt taaaaataaa tggcatgttg gcgctaacgc ccagggcaga
     2401 aaaaaacttt ccattcggga gaaaaccact ttgatatgcc gggccaataa acattccgta
     2461 tagaaataat ctgttgcgca ctgcataccc tgcagcaata tgaaatgagt ataaggagaa
     2521 ctcgctttgg taaatcacaa aactctgaac accaccctgc aaatacattt ttccaactgc
     2581 acgtgtgtaa tcaaatgtat aattccgtcc attaccaaaa cttcccaatc gttccatagt
     2641 gaaacctacg ccaaggtttc cccattcgta atgttttaca ggctttttat ttgtggttga
     2701 ttgcgcatct aaatattgca caacaaacat gaatgccatt gtcaatatta taacattaac
     2761 cttataccaa ggctttgatt tgataagcat attttgtgta aaattcaatt gtgacttatt
     2821 ggtctttacg ttcaatagcg ccaatatcgg caggtaaaaa catattgcga tcggtagttt
     2881 ttaaatcgta ttgcaatatt ggatgcaatg gagaacagca agtgctatca gctttgtttt
     2941 ttgcaaatgc cattgtatcg agcgaaaaat cgccatcgtt ggtattctta aaatcgggat
     3001 cgctgttttt atatatcatt ttaaaatgat taggatctgt aatgctaaaa tccgatttca
     3061 cttttataat atcatggtcg aaaaaataaa aatcatcacc accaggtttc atttccactt
     3121 caatttcgtt ttcgttgttg ccataaatta tgcaattgcg aaacaatgct ttatgcatat
     3181 cgaagctaat aatattatcg ttattatcta atgtataatt gttgagataa atggatggag
     3241 tagtgcgttg cccatatttc caatagttgg caaacgtgca atgattaaat tcgtaagtgc
     3301 cgccaagcga taacgctgca caatacgaag cacaattcga aatcacacaa ttattcatta
     3361 cgaagttatg ataacgcgca aatacaccgg caccactcat gttagaaatg gtagtattat
     3421 ttaccataac agatttgtca ttactggtat cggaattatt atcgaacagg gtttcgattt
     3481 gtaatccaat aaagccattt tttatttcgg cataatttat ttcgttgccg aatgcgccat
     3541 catttatcca aatgcgatcc cattgtccgg gcttgtcggc atagtaactt tcgaggcgtg
     3601 taccaccaaa ttttacaggt tcatctttgg tgcccattat ttttatacaa ccaccacgat
     3661 aaacccacaa gccggaacca ttataaaaat aaatttttgt gcccttagaa attttcaaaa
     3721 cgcaaagtga atcgacaacg gcataaccaa aaataacata aggcaaaaca ttatcccact
     3781 cagctaaatt atttacgcat ggaataatag aaagaggagg gaggccggga atatttaaat
     3841 cggcacgaat gaaatgagca ttttgtcccc atgcaactaa atcaacatcc tgaatatttc
     3901 cattggtttc aaaaataatg gagtcggcaa caatgaatgg aagattggca gacgaaggat
     3961 cgatggtgac ttcaacaaaa ataaacatac tatcttttgc accaatctca atgtcactaa
     4021 attttgtgcc cgatacaccg tctacattca gccggaaata ggaattattt gtatttgaca
     4081 agcgtatact tgatatgttg ataggttgcg aattgttatt ataaaccagc aactgttgat
     4141 tggtagaacc gaaggttaca aacacggtat cgaacacaat agtatcggca ctgaaatcta
     4201 attttgcact tgcatcggta atgagttttt cttttttaca agaattaaaa aatatcatgc
     4261 ccaaggcaaa tgccaaaaca gtaaaacctt tgagtgcaaa aaataaatgg tgtacgtact
     4321 tattgagtat tgacttcatg tgacaaaagt aaaaatattg atacataata taatgtatga
     4381 agtgaaagat tattgtgatg gagctatttt atgacacatt tatgttgcaa tgcaatccat
     4441 atttgattta gcattgtgta aaaatgaatg tatggaaaag aaatcactaa aattcggtgc
     4501 taggatttta ctcttcatat gttcgtgaat agccgcgcca cttttcaatc agcgtgttaa
     4561 agttttcggg caaagggctt tcgaaaaaca tttccttctt tgtaacagga tgcacaaaac
     4621 ctaaagtggc agcatgtaaa gcctggccgg gaagcaacgc aaaacaattt tgtacaaatt
     4681 gtttgtatcg tgtaaaagca gtgccgcgca aaatagcatc accaccatat tcgaaatcgt
     4741 taaacaaagg atgctttatg tatttcatgt gcacacgtat ttgatgtgtg cgtccggttt
     4801 caagtttgca ctcgatgagg gtggtgtata aaaatttttc taaaacttta taatgtgtca
     4861 cagcatgttt tccatattcg ccatttggaa atacatccat catttttcga tttgcaatat
     4921 tgcgtcccac atttccggtt acagtgcctt cagcattgtc aaaatcgccc cataccaaag
     4981 caatatattt tcgttgtgat gtacgttcga aaaattgttt tgctaaatgt gataaagcat
     5041 attc
//
LOCUS       DAORQA010000125         4974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4220_length_4974_cov_4.667209, whole genome shotgun sequence.
ACCESSION   DAORQA010000125 DAORQA010000000
VERSION     DAORQA010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4220_length_4974_cov_4.667209"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(364..1185)
                     /gene="panB"
                     /locus_tag="PK736_06755"
     CDS             complement(364..1185)
                     /gene="panB"
                     /locus_tag="PK736_06755"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRC33126.1"
                     /translation="MSASQNSEIKRITTHRLLEMKIKGEPIAMLTAYDFSMAGIIDAA
                     GIDIILVGDSASNVMAGHETTLPITLDQMIYHASSVVRAVSRALVVVDLPFGSYQGNS
                     KEALNSSIRIMKESGAHAVKLEGGRQIKESIERILSAGVPVMGHLGLTPQSIYKFGTY
                     VVRAKEEEEAKQLIEDAHILQESGCFSIVLEKIPAQLATRVSQELRIPIIGIGAGNGV
                     DGQVLVLHDMVGINNEFHPRFLRKYANVHEVMLNATKKYIEDVKSKDFPNEKEQY"
     gene            complement(1552..2682)
                     /locus_tag="PK736_06760"
     CDS             complement(1552..2682)
                     /locus_tag="PK736_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33127.1"
                     /translation="MASIILNRKTAVSPFAILSGFETNFEMYRLVTSLKVEKTGSDET
                     ALGILFMAQPNNKGGYMFEINKFKEYRIRRMEGAAFKNITGDAKNSGWVKHPTIVDLN
                     LPNLVEIRTANSNFDLFINNTFVQTFNDNMYSTGKMGLIVGPATKGRVDFFYVFTNSK
                     PADNVTTQTTKSNNDDVDISALAESIINLKTQINKLKEDNGMLRKTIDAMKGEDGDID
                     ALKKSHTAQLESLQNQLSQTQTRLDSTNNANKELVKYRDMIKGDENSDLLITLSKTVK
                     TERDKNVAMQEENKQLRDSLEMIRAAVKSILGNSIESLSNPKNPPPVKSNTGKPNNQQ
                     PPPKTGGNLPAPLMDDESYFVPQFLPSIIHLMKYKNLEIMLA"
     gene            complement(2657..2788)
                     /locus_tag="PK736_06765"
     CDS             complement(2657..2788)
                     /locus_tag="PK736_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33128.1"
                     /translation="MIHEKFDKLVVSENFDQSNNNWSTVSNNDNLFVMQDGEYYPQS"
     gene            complement(2978..>4974)
                     /gene="cphA"
                     /locus_tag="PK736_06770"
     CDS             complement(2978..>4974)
                     /gene="cphA"
                     /locus_tag="PK736_06770"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRC33129.1"
                     /translation="TKNLLEAQSIPVPRGRIIYSEEELKDAVEDIRFPVVLKPVNGNH
                     GRGATINVNSWDDAVTALAAAKRISKGVIIEKYITGYDHRVLVINYKLEAAAKRTPAA
                     VIGDGKSTIQQLVDKVNADPRRGYGHEKVLTTIKIDDTTNSILAEKSLTLDSVLKDGE
                     ELWLKRTANLSTGGTSTDVTDVMHPYNIFMCERIARIIGLDICGIDIMTPNISEPLNE
                     IGGAVLEVNAAPGFRMHLSPITGLARNVAAPVVDMLFPPGSNARIPIVAVTGTNGKTT
                     TTRLIAHMCKFMGHKVGFTTTDGIYIQNHLLQKGDCTGPASAEFVLKDPTVDFAVLEC
                     ARGGILRAGLGFHKCDIGIVTNVSADHLGLKGINSLEDMAKVKGVVVESIMPSGYAIL
                     NADDQLVADMARPLECKIAYFSLDENNAIIKRHTANGGLAAVAENGFITIMRGTWKIR
                     VDKVVNIPLTFSGKAIFMIQNILPAVLTGYIRNFKIEDIKLALETFIPSPAQTPGRMN
                     LFQFKKFTVMVDYAHNPAGFQAIAKFLDKVEAKPKVGIIAGVGDRRDEDILQLGQLSG
                     NMFDEIIIRQDRNLRGRTEKEIIDLLLVGIHRDGKEKIVKVIPTEYEAIEYAITNAKK
                     GSFITICSDVVPDALAQIMKYKEEEDKFEITAQDIPNSGV"
BASE COUNT         1418 a         1015 c          903 g         1638 t
ORIGIN      
        1 tacgggtgag cgcaggcaat tggatttaat tccttaactt aatgacattg ccctccggga
       61 ctttcaacgg ttttagatta ttgtgagttg atgcttatac cattcagggt tctcatagta
      121 cgtatatctt catcaaccac agggattgag ctgtggttat tcaagttaaa ccatttcaag
      181 gttattattt tttttgcata ggattgtgtg tgaagaattt atggtacgaa aactagaact
      241 atcttatcat gtaagacaat taaaaagcaa ttgtgtatta caaaaaaaat caaatcgcaa
      301 attgagctat cagaaaatca tcacaaggcg tcattctttc gtcaattatt ttttaagcac
      361 aaatcaatat tgttcttttt cgtttgggaa atctttcgac ttcacatctt cgatatattt
      421 ttttgttgcg ttaagcatta cttcgtgcac gtttgcatac ttgcgcaaaa accgcggatg
      481 aaattcatta tttattccta ccatatcgtg cagtacgagc acctgaccat caacaccatt
      541 gcctgcacct atgcctatta ttggaatccg caattcttgc gaaacgcgtg tcgccagctg
      601 tgcaggaatt ttttcgagca caattgaaaa gcaccctgac tcttgtaaaa tgtgtgcatc
      661 ttctataagt tgtttagctt cttcttcttc ctttgcccta acaacataag ttccgaattt
      721 ataaatcgat tgtggtgtaa gtcccaggtg acccatcaca ggcacacctg ccgataatat
      781 tctttcaatc gattctttga tttgcctgcc gccttcgagt ttcacggcat gtgctccact
      841 ctctttcata atgcgaatgc ttgagttgag tgcttctttg gagtttccct gataagagcc
      901 aaagggcaaa tcgacaacta ccaaagccct tgaaactgcc cgcaccaccg atgaagcatg
      961 atatatcatt tggtcaagcg taattggcaa tgtggtttcg tggcctgcca ttacattgga
     1021 ggccgaatcg cccactaaaa tgatatcgat gccggctgca tctattatac ctgccatgct
     1081 gaaatcgtaa gcagtaagca tggcaatagg ttcgcctttt attttcattt ccaacagacg
     1141 atgtgtggta atgcgtttaa tttctgaatt ttgtgatgca ctcattttta ttattttggt
     1201 gagcaaatgt aattatttcg ctatagtaca atagtagaat ttgtgggcaa taatattata
     1261 ttcgaaaatg acttttggaa tgaaaaaaaa atgacaacaa aagaaacgag cagcatttat
     1321 taatgtataa taccattgct tttataaaac aggcttttct tacctcaccc caaccctctc
     1381 caaaggagag ggagaaatcg cgttagccta ttgtataata gctaatggca caaaatacta
     1441 accataacag tatacgatgg actactttca ttagtttgac aaaaaacgcc agtgaaaaaa
     1501 ttcaatttat aacgaaaaaa acataagcaa gattataaaa ctaaatcagc gtcaagcgag
     1561 cataatttcc aggtttttat atttcataag atggataata gatggcaaaa attgaggcac
     1621 gaaataactt tcatcgtcca tgagtggcgc aggtaaattt cctcctgttt ttggtggtgg
     1681 ctgctggtta tttggcttgc cggtattact ctttaccgga ggcgggtttt tgggattact
     1741 aagactttcg atggagttgc caagaatact ttttactgct gctctgatca tttcgagcga
     1801 atcgcgcaat tgtttatttt cttcctgcat ggcaacattt ttatcgcgct cggtcttcac
     1861 tgttttcgat aaagtaataa gcaaatcgga attctcatca ccttttatca tgtcgcgata
     1921 ttttaccaat tctttattag cattattggt actatctaat cgcgtttgcg tttggcttaa
     1981 ctggttttgc aacgactcca attgagcggt atgtgacttt ttaagcgcat ctatatcacc
     2041 atcttcgcct ttcattgcat caatagtttt gcgcaacatg ccgttatctt ctttcagttt
     2101 attaatttgg gttttaagat ttattattga ttcggctaat gcagatatat caacatcatc
     2161 gttgttgctt tttgtagttt gggtggtaac attatctgca ggttttgaat tggtaaacac
     2221 ataaaagaaa tcgacacgtc cttttgtagc aggacccact atgagtccca ttttaccagt
     2281 gctgtacata ttgtcgttaa acgtttgtac aaaagtgtta ttgataaaca aatcgaaatt
     2341 agaatttgct gtacgaattt caacaaggtt aggcaaattt aagtctacaa tggtaggatg
     2401 cttcacccat ccgctatttt ttgcatcgcc agtaatattc ttgaaggcag caccttccat
     2461 cctgcgaatt ctatattcct taaatttatt tatttcgaac atgtaacccc ctttattatt
     2521 gggttgtgcc ataaataaaa taccaagagc ggtttcgtcc gatccggttt tttctacctt
     2581 caacgaagtt accaggcggt acatttcaaa gttggtttcg aaaccactta gaatagcaaa
     2641 aggtgaaaca gcggttttac gattgaggat aatactcgcc atcctgcatc acgaataaat
     2701 tatcgttatt ggaaactgta ctccagttgt tgttgctttg gtcaaagttt tcgcttacca
     2761 ctaatttgtc aaacttttcg tgaatcattt gactttttgt tttgtgaaac attaatatgc
     2821 acaaaacaat tataccaaca gatttaactt tagatatcat tgcatttttt atttctatca
     2881 acttttcaga catgatgggg acatcatttg ccttaaagaa cagaattaaa tttataaatg
     2941 cctaatactt cgccaaaaat aaaatagcta attaaattta tactccggaa tttggaatgt
     3001 cctgagctgt tatttcaaac ttatcttctt cttccttata cttcattatt tgtgcaagtg
     3061 catcgggaac cacatcgctg caaatggtaa taaatgagcc ttttttggca tttgtaattg
     3121 catattcaat ggcttcgtat tcagtaggaa taacttttac aattttttct tttccatcgc
     3181 gatgaatacc caccagcagc aagtcgataa tttccttttc ggtgcggcca cgtaaattgc
     3241 gatcctgacg aatgataatt tcgtcaaaca tatttccact caattgtcca agttgcaaaa
     3301 tgtcttcatc acgtctatcg ccaactcctg ctataatacc aactttgggt ttggcttcca
     3361 ctttatctaa aaactttgca attgcctgga aacctgccgg attatgggca tagtcaacca
     3421 taacagtaaa tttcttaaat tggaaaaggt tcatccttcc aggggtttgt gccggtgaag
     3481 gaataaatgt ttccagtgcc agtttaatat cttcaatttt aaaattacga atgtatccgg
     3541 taagtactgc aggtaaaata ttttgaatca tgaatatggc cttgcccgaa aaagtaagtg
     3601 gaatatttac aactttatcg acccgaattt tccaggtgcc acgcataatg gtgataaaac
     3661 cgttttcggc aacggctgcc aatcctccat ttgcagtgtg acgtttaata attgcattgt
     3721 tttcatcgag tgaaaaataa gcaatcttac attccaaagg acgggccata tcggcaacca
     3781 gttgatcatc ggcattgaga atggcataac cacttggcat tatactttct acaactacac
     3841 ctttcacttt ggccatatct tcgagtgagt ttataccctt cagtccaaga tgatcggccg
     3901 aaacattagt tactatacca atatcgcatt tgtgaaaacc taatcctgca cgcaatattc
     3961 ctccacgggc acattcaagt acagcaaagt caacagtagg atcttttaac acaaactcag
     4021 cacttgcagg ccctgtgcaa tcgccctttt gcaacaaatg gttttgaata taaattccat
     4081 cggtagtggt aaaaccaact ttgtgcccca taaatttaca catgtgtgct atcagtcggg
     4141 ttgttgttgt cttaccattc gttcctgtta cagcaacaat tggaatacgt gcattgcttc
     4201 ccggaggaaa taacatatcg acaaccggtg ctgctacatt gcgcgccaac ccagtgattg
     4261 gtgataagtg catacgaaaa cccggtgcgg catttacctc aagtaccgcc cctcctattt
     4321 cattcaaagg ttcacttatg ttgggtgtca ttatatcgat accacatata tccaaaccaa
     4381 taatgcgtgc aattcgttcg cacataaaaa tattgtatgg atgcataaca tcggtaacat
     4441 ctgtcgatgt tcctcctgtg ctaagattgg cagtacgctt gagccataac tcttcaccat
     4501 ctttcaaaac agaatcgagg gtaagcgatt tttcggcaag tatgctattt gttgtatcat
     4561 ctatcttgat ggtggtaagt actttttcgt gaccgtaacc tctgcgcgga tcagcattta
     4621 ctttgtcgac taattgctga atggtgcttt tgccatcgcc aattaccgca gcaggagtgc
     4681 gtttggcagc ggcttccaat ttataattta ttacaagtac ccgatggtca tatccggtaa
     4741 tatatttttc aattattaca cctttggata tacgttttgc agcggcaagg gctgtaactg
     4801 catcgtccca agaatttaca ttaatggttg cgcctcgccc atgattacca ttaacaggtt
     4861 ttagcaccac aggaaaccgt atatcttcca cagcatcttt taattcttct tcactatata
     4921 taatgcggcc acgaggaaca gggattgatt gtgcttctag cagattcttg gtat
//
LOCUS       DAORQA010000126         4937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4258_length_4937_cov_37.627612, whole genome shotgun sequence.
ACCESSION   DAORQA010000126 DAORQA010000000
VERSION     DAORQA010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4258_length_4937_cov_37.627612"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PK736_06775"
     CDS             <1..761
                     /locus_tag="PK736_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRC33130.1"
                     /translation="SFIIEGEFRTYFKHLRVNKEIKQLKGHVVVCGYGRNGKQACQQL
                     TSGGVKYVVIEGNPQIIEQLKAEITTLFVEGNATEDEVLEMAGIKQAKGLITTLPDDA
                     DNVFVVLTANELNPALKIISRASSDGAENKLKRAGADNVIMPDKIGGTHMAALITKPD
                     VLEFLDFITGKINIRLEEIHYNNLSDHHKDKSMQQIGVPGNSGANIIGFKKDNGDYIV
                     NPPAVTIMTPNAKLFVLGTQEQVAAFLNILKDNL"
     gene            complement(1357..1959)
                     /locus_tag="PK736_06780"
     CDS             complement(1357..1959)
                     /locus_tag="PK736_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRC33131.1"
                     /translation="MPHIKHLSKDKILKQAIEKTGRLELSTRKNIFLSLCNSIMSQQL
                     STKVADIIQARFVNLFDDKKPIPEKVLQLSHETLRSIGLSNAKVTYVQNVARFALEKG
                     MNASKLNKMSNEEVIDYLTEIKGVGRWTVEMLLMFTLGREDVFAVDDLGIQQAIVSLY
                     NIENTEPKKIKIKMQLLAENWAPYRTYACMYLWRWKDGVK"
     gene            2123..4201
                     /locus_tag="PK736_06785"
     CDS             2123..4201
                     /locus_tag="PK736_06785"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRC33132.1"
                     /translation="MKKYLLCLFLATYVVTVSSQTFVGGGGTIPDNGPAAFFPIVVSS
                     LSPNVIDSTFGLESVCININHNNVYQLTIQLMAPDGTTIDLSMNNGSTGNNYTNTCFT
                     ATAINSITNGIAPFTGNYKAQGNIGNVNNNQNGNGIWQLKVHDHYAFSIGSVISWSLF
                     FSNAPAYPTAFTSSNLPLIIINTNGQTIVDNPKKIVHMGIIDNGVGVRNYLTDPFNNY
                     NGNIAIEIRGSWSQTFPQQQYGFETLDAANIEADTMVLGMPAQSDWILYAPYNDKTCM
                     RNVLAYDIANKTGHYASRTRFCELIINNDYLGIYVMEEKVKRDSNRVDVSKLLPTDTA
                     GNDITGGYIVKIDKTTGAGGSAGWNSKYDGVPGTKIQFLFEYPSSVNIVAKQKAYIQA
                     YVDSFERALFSPWFLNTDSGYKNYIDVQSFVDYFILNETCKNVDAYRISTFLYKKKNT
                     KGGKLFVGPAWDYNIAFWNADYCEGYLTTGWQYQYNNICGSQGGDNVPFWWTKMLQDP
                     IYTDMLKCRWTQLRQSTLHTDTLLNLIDTMAMLLDESKDRHFTKWPVLGTVLWANPQP
                     VANDYAGEISNMKTWIQQRILWLDANMPGSCTGLAIDDRNYDLQQIGAYPNPFTNKLN
                     VNFYVVKPGDVIIEITDIAGRIIYNHNATYTVTGKQTLVINNFSFGSGIYILKVETET
                     GVSTIKIVRK"
BASE COUNT         1571 a          898 c          942 g         1526 t
ORIGIN      
        1 cgtcttttat tatcgaagga gaattcagaa catactttaa acatttaaga gtgaataaag
       61 aaataaaaca attgaaaggc catgttgtag tttgtggata cggacgcaac ggcaaacagg
      121 catgccagca acttacatcg ggtggtgtaa aatatgttgt gatagaaggc aatccacaaa
      181 ttattgaaca attgaaggct gaaatcacaa cgctatttgt tgaaggaaat gccactgaag
      241 acgaagtgct ggagatggca ggaataaaac aagccaaagg attgataaca acattgcccg
      301 atgatgccga taatgttttt gtagtactta ctgcaaacga attgaatccg gctttaaaaa
      361 ttataagccg tgcatcgagt gatggtgccg aaaataaatt gaaacgtgcc ggtgccgata
      421 atgtaataat gcccgacaaa attggtggca cacacatggc cgcacttatt accaagcccg
      481 atgtactcga gtttttggat tttatcaccg gcaaaataaa tatccgcctc gaagagattc
      541 attacaataa tttgagcgat catcataaag ataaatcgat gcagcaaatt ggagtgcccg
      601 gtaattcggg tgctaacatc attggcttta aaaaagataa tggcgattat attgtaaacc
      661 ctcctgccgt tacaataatg actcccaatg caaaactttt tgtgcttggc actcaagaac
      721 aagtggcggc atttctcaat attttgaaag ataatttgta aagctttttt tagaattatt
      781 ttttaaaaaa ttaaattgct tacagacgaa ttacaaaaag caatttgatg gagtatgtga
      841 taagccaaga tacacatcat cacaaaaaaa tttgaaaaag agtagactga attattaaaa
      901 ggacacgaag tgctgcatga cgaaaatatt tggagcagca gcagttcaac ccttacaggg
      961 ttggcttgta tagcgtttgc agcttctccg cgtttcacat ggagttattc aagttgaagc
     1021 cctccgggct tttcaacaca aaagaaaatt agtaaacgat atagatgccg accaaccacg
     1081 aagtggttca acatgaataa ccccacatga aatgtgggga atcagcaaaa ggcaagactc
     1141 ctaaccacgg agttgttaaa cgtgaataac cccacatgaa atgtggggaa agagcaatag
     1201 gcaacgcacc taaccacgga gttgttaaac gtgaataacc ccacatgaaa tgtggggaaa
     1261 gagcaatagg caacgcacct aaccacggag tggttaaaca aaattttatt tggctgaaat
     1321 gcccattttt tttaaagcca aatgttcata gcatttttat ttcacaccat ctttccatcg
     1381 ccataaatac atgcaagcat aagtacggta tggtgcccag ttttcggcaa gcaattgcat
     1441 ttttattttt atctttttgg gttcagtatt ttctatattg tacaaactta ctattgcttg
     1501 ttgtattcct aaatcatcca cagcaaacac atcttcgcgg ccaagagtaa acatcaatag
     1561 catttcaact gtccagcggc caacaccttt tatttcggtg agatagtcaa ttacttcttc
     1621 gttgctcatt ttatttagct tacttgcatt cattcctttc tccaatgcaa agcgcgccac
     1681 attttgcaca taggtaactt tggcattgct caatccaatg ctgcgcaatg tttcgtgcga
     1741 tagctgcaat actttttcgg gaataggttt tttatcatca aacaaattta caaatcgtgc
     1801 ttgtataata tcggcaacct tggttgatag ttgttgagac atgatggagt tacacaaact
     1861 caaaaatata tttttgcgtg tgctcaattc caacctgccc gttttttcaa tggcctgttt
     1921 taatattttg tctttcgata aatgttttat atgtggcata gattgatgat ttatttttca
     1981 aacataggca ttttcattca cgaattttgc attctgcttt ttagatgttt tcatcctaat
     2041 tcattttata agatggcatt attgtatctt ataataattt tctcttctat aaattcttaa
     2101 atattattac catctttaac caatgaaaaa atatttactc tgtcttttct tggcaacgta
     2161 tgtagtcacc gttagttcac aaacttttgt tggcggtggt ggcactatcc ccgacaatgg
     2221 cccggcagca ttttttccaa ttgtggtaag tagcctttcg ccaaatgtta ttgatagcac
     2281 ctttggtttg gaatctgttt gcataaatat taatcacaac aatgtttatc aacttaccat
     2341 tcagcttatg gctcccgatg gcactaccat agacttatcc atgaacaatg gaagcaccgg
     2401 aaataattat accaacacat gctttacggc aactgcaatt aatagtatta caaacggaat
     2461 agcaccattt actggcaact acaaagcaca aggcaatatt ggcaatgtca ataataatca
     2521 aaatggaaat gggatttggc aattgaaagt gcatgaccat tatgcgtttt ccataggttc
     2581 ggtaatttca tggagcttgt tttttagtaa cgctcccgcg tatcctactg cgtttacatc
     2641 gtccaatttg cctttaatta tcattaatac caacggacag actatagttg ataatcctaa
     2701 aaaaattgta cacatgggta ttatagataa tggagtagga gtgaggaact acctcaccga
     2761 cccgtttaat aattacaatg gaaatatcgc catagaaatt cgagggagtt ggtcacaaac
     2821 ttttcctcaa cagcaatatg gtttcgaaac gcttgatgcc gctaatattg aagccgatac
     2881 aatggtactt ggaatgcctg ctcaaagtga ctggatattg tatgcaccct acaacgataa
     2941 aacttgtatg cgcaatgtgc tggcatatga tattgcaaat aaaacagggc actatgcatc
     3001 gcgtactcgc ttttgcgagt tgataataaa caacgattac ctgggcattt atgtaatgga
     3061 agaaaaagtg aagcgcgatt cgaaccgtgt tgatgtatcg aaactgttgc ccaccgatac
     3121 tgccggcaat gatattaccg gaggctacat tgtaaagatt gataaaacaa caggtgcagg
     3181 tggtagcgca gggtggaatt cgaaatacga tggagtaccg ggcactaaaa ttcagtttct
     3241 atttgagtat ccatcttctg taaatattgt ggcaaagcaa aaagcataca ttcaggctta
     3301 tgtcgattcg tttgaacgtg ctttgttttc tccctggttt ttgaatacag atagtggcta
     3361 caaaaattat atcgatgtgc aatcgtttgt cgattatttt atactaaacg aaacatgcaa
     3421 aaatgtggat gcctatcgca taagtacatt tttgtataaa aagaaaaata ccaaaggcgg
     3481 aaaattattt gttggcccgg catgggatta taacattgca ttctggaatg ccgattactg
     3541 cgaagggtat ttaacaacag gctggcaata tcagtacaat aatatttgtg gctcgcaggg
     3601 gggcgataat gttccattct ggtggacaaa aatgttgcaa gacccaatat ataccgacat
     3661 gcttaaatgc cgctggacac aactcaggca gtcaacgctg cataccgata cattgctcaa
     3721 tctgattgat acaatggcca tgttgcttga cgaatccaag gacaggcact ttacaaaatg
     3781 gcctgtgttg ggcacagtat tgtgggccaa tccccagcct gttgccaatg attatgccgg
     3841 tgaaatatcc aatatgaaaa cctggattca acaacgaata ctttggttag atgccaacat
     3901 gcccggctca tgcacaggcc tcgccattga tgataggaac tatgatttgc aacaaattgg
     3961 tgcctatcca aatccattta ccaataaatt gaatgtgaat ttttatgttg taaaacccgg
     4021 agatgtaatt attgaaataa cagacattgc cggtcgaatt atatacaatc ataatgcaac
     4081 gtatacagtc acaggtaagc aaactttggt tataaataat ttttcatttg gttctggaat
     4141 ttatatatta aaagtagaaa cggagaccgg tgttagtacc attaagattg taaggaagta
     4201 attccagtta ctttatttca gctgcgtaaa taactttttc caattctttt tattcaattg
     4261 tattaaattg cattgttttt caattcaata cattttaaat tcaagaaaca cattttatga
     4321 aaatgaaaat caagattctg tgccttttct ttgcaatttt gcgcttatgc aattaattat
     4381 tgcacaagat ggttcgaaat gatagctgaa ttaaatgccc cgaatttaaa ctgtgcattg
     4441 cattcttaaa aaggtaaatc acagcatcgg aaaggtggta tttatattgg ttgggcaaat
     4501 tgcttgcttg tgtattcaaa tcaaaattgc cggagggtat atgttcgaat ttgtatacat
     4561 tgttgcaaga attgaaagtt gatgctgtca aaatcatgat gcagcatata atcagtatat
     4621 ttggttttac agttatcatt gtgtgtgttg ttttttaaag ccataattat tcagcacata
     4681 aaaacgtaat attatatttc aactgctatg aataataaaa attataattg ccaaatcatc
     4741 aatcggatga aataaatgtt cctaaatcat tccggtcatt tttgccaatt gcacaagggc
     4801 tgtagtatcg cgtgcgccta ttttttcaat catattactc cggtggttgc caacagtggc
     4861 cgtgcttata aaaagttgtc tggcaatttc tttggtatcg gtatcggccg caataagttt
     4921 tagtatttct ttttcac
//
LOCUS       DAORQA010000127         4892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4309_length_4892_cov_10.252843, whole genome shotgun sequence.
ACCESSION   DAORQA010000127 DAORQA010000000
VERSION     DAORQA010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4309_length_4892_cov_10.252843"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..2145
                     /locus_tag="PK736_06790"
     CDS             124..2145
                     /locus_tag="PK736_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRC33133.1"
                     /translation="MKKLMVLAIIIWLDSTCVFAKFSETKAFAHFQSGDDKLSDKEKL
                     KLKDFISTTNSTMAYLEFVITGFADADGSTEYNRALSERRAIEVRNYFIANGIDDKII
                     NYKYHGEQQPISTNADENGKSKNRRVEIALRKYEFEKATEIIKELNGDVQQTFEIDNT
                     KKAEVKTSSGTKFIFPENSFCFSDNRNKIASKVTITITEVRTVSDAMFNNVLAQSDEG
                     MLVSGGMYNIQAVSNKLPLQMVPGKNYTVQLANQTIEQGMKVYTSVPLPNGLVSWKNS
                     NADFIAKSKALSSQLFISLDENIVYKWQPIEGAVDVPVYTTQILPTRVAALATNTRPR
                     KPSQFNHIFGGYTKWYKKLFTSKKRQFAREQKLYSRQMKNYEKSYGRYMDKLVKFEDD
                     SLRNISIANSLEKAISNYDSTLTFQYNLYMNCKLALQKNKLHQMFYVAKQQLLFNSKS
                     NRLTNNPLTAMRTMLSEYIYPKRADMSLENYVGYEKELILRMTESGLPTSMRFYNAET
                     QSYKTQLELYYENNFEAVAPKEYFKDITLALREKQIAANTLSTNDFRTYYQSEMSTLG
                     WINCDRFEQNKQRPMVSLNVNDLNLKDENVMLIVNKYKSNMAMNRNEKGYSIKLPKGE
                     NVTLLVIGMDKNNQPMFYKKTFNVNNNTVVPIDLQKISLALLATELKNL"
     gene            2449..3417
                     /locus_tag="PK736_06795"
     CDS             2449..3417
                     /locus_tag="PK736_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33134.1"
                     /translation="MIRYLRHNEIDKPKWDDCIQNATYSIVYAYSWYLDIVSPNWDAL
                     VEDEYNTIFPLTWKKKYGMMYLTQPLFAQQLGLFSTEEISTKKIAMFLKKIPHKFIHW
                     DISFNPSNNALNSYIVYSIKRLTYHISLRQSYDEIHHKYSHDTKRVLKLNHQHDFVIS
                     NILCEEVLKLYKQNVWHKVPVVTLDNFETLKRLVNELCNKNICKVQGIFEQDKIAGSA
                     VLINCDNKLIYLFGAANESGRRSGAMRYYFDNMIKQNCNKNLILDFEGSIFPTVAHFY
                     KSFGSNKIEFFNLRFSRIPILNLMIKFKQGIKQKLSAYRIMHSTSV"
     gene            complement(3424..4836)
                     /locus_tag="PK736_06800"
     CDS             complement(3424..4836)
                     /locus_tag="PK736_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33135.1"
                     /translation="MKKLYILFLITATFANAQQINFTKTYFPGLYADVRGIAQTPDCG
                     FLVAGLDKTSTDTVGDMYVMKIDANGNSQWKKYYGLPKEDGGNDIILCNDGGFIVSGH
                     TNNLGDECDMYLIRGDVTGNTIWSKQLGGFYDDIAITSIEIPDGNFITLGNTENYGAT
                     NLDFMLARMDKNGNEIFVKTIGTEKRDFAKCMIRTSDNSLILGGSSYNNSTMEYSMTL
                     IKCDLNGNILWTNNNYNSLESNGIYSILESPQHNLYCIGRKYSADNGMQGYVACLNLN
                     GDVIWEKEFGSKLYDAFTDACILASGSIYCVGETCNFGDEQGDIMVVKMDYLGNFTAP
                     HLYGTKNIKDEARTVVVCSDGGLVIGGQSNITAGTYEALVIKTGVWASGINDFTFSNS
                     AVYAYPNPFSDKVNFSTYYDASSTKELKIFDAKGQVVCSHLFSQPFLKLELNMLQNGV
                     YYYQVSDKGAILKSERMIKQ"
BASE COUNT         1738 a          833 c          804 g         1517 t
ORIGIN      
        1 tctataaaca tcctgaattc gattttaaca aacccgaaac tttaacacag ggagggcgag
       61 tcatctacaa tattataata acattcatcg tatattaaca ataacttaaa tctttaaata
      121 attatgaaaa aacttatggt tttagccatt ataatatggc ttgattcgac ttgtgttttt
      181 gcaaaatttt ccgaaacaaa agcgtttgct cattttcaat cgggcgatga taagctgagc
      241 gataaagaga agttaaagct taaagacttt attagcacaa ccaattctac tatggcatat
      301 ttagagtttg tgattactgg atttgcagat gcagatggaa gcacggaata caatcgtgca
      361 ttatcggaac gcagagcaat tgaagtgcgg aactatttta ttgccaatgg catcgatgat
      421 aaaattataa actataaata ccatggtgaa caacaaccaa taagcaccaa tgcagatgaa
      481 aacggcaaaa gcaaaaaccg aagagtagaa attgctttga gaaaatatga gttcgaaaag
      541 gcaactgaaa taataaaaga gctcaatgga gatgtgcaac aaacatttga aattgacaac
      601 actaaaaaag cagaagtgaa aacaagcagt ggtacaaaat ttatttttcc tgaaaatagt
      661 ttttgctttt cggacaacag aaataaaata gcatcgaaag taactataac cataaccgaa
      721 gttcgaacgg taagcgatgc catgtttaat aatgtattgg cgcaaagcga tgaaggcatg
      781 ttagtttcag gaggtatgta taatattcaa gcagtttcga acaaattgcc attacagatg
      841 gtgccaggta aaaactatac agtgcaattg gcaaatcaaa caatagagca gggcatgaaa
      901 gtttatactt cggtgccact tccaaatggg cttgtgagtt ggaaaaattc caatgctgat
      961 ttcatagcaa aaagcaaagc gttgtcatcg caattattta tttcgctcga tgaaaacatt
     1021 gtgtacaagt ggcagccaat tgaaggtgcg gttgatgttc cggtatacac aactcaaata
     1081 ttacctacaa gagtagcagc tttagccacc aataccagac cacggaagcc ttcgcaattc
     1141 aatcatattt ttggtggtta cactaagtgg tataaaaaac tatttacatc gaaaaaaaga
     1201 cagtttgcca gagaacaaaa attatattca aggcaaatga aaaactacga aaaatcttat
     1261 ggcaggtata tggataaatt agtgaaattt gaagatgatt cactgcgcaa catttcgatt
     1321 gctaatagtc ttgaaaaagc aatttccaat tacgattcta ctcttacttt tcaatataac
     1381 ctttacatga actgcaaact cgcattacaa aaaaataaat tacatcaaat gttttatgta
     1441 gcaaaacagc aattgctttt taatagcaaa tcaaatagac tgaccaataa tcctcttact
     1501 gcaatgcgca caatgttgag tgaatatatt tatcctaaac gagctgacat gtcattggaa
     1561 aattatgtag gctacgaaaa agaattaata ctgaggatga ccgaatccgg tttgccaacc
     1621 agcatgcgat tttacaatgc agaaacccaa agttataaaa cacaacttga attgtattac
     1681 gaaaacaact ttgaagctgt agcaccaaag gagtatttta aagatattac acttgcactt
     1741 cgcgaaaaac aaatagcagc aaatacatta tctacaaacg attttcgcac atactatcaa
     1801 tcggaaatgt cgactctggg ctggattaat tgcgatcgct ttgaacaaaa taaacagcgg
     1861 cctatggtaa gtttaaatgt gaatgatctt aatttaaaag atgagaatgt aatgttgatt
     1921 gtaaataaat ataagagcaa tatggcaatg aatagaaacg agaaaggata ttctatcaaa
     1981 ttgccaaaag gagaaaacgt tacacttttg gttattggta tggataaaaa caatcagcct
     2041 atgttttaca aaaagacttt taatgttaat aacaacactg tagttcctat cgatttacaa
     2101 aaaataagtt tggcgcttct cgcaacagag ctgaaaaatt tgtagcatga ttgtaattgc
     2161 aaaaatgaaa attagtcaat tcaattaaaa tatttgcaaa catcatttct taaccggacg
     2221 atattattgg tattgtttta ttaagctgat tagcgttttg aatttggcgt gaagcaccat
     2281 cattattttg aaataaaata gtcctggtgc ttttctttct aagtagcaat tacatttcac
     2341 gcatcgaaag tattttaaat atagtttcat cgttaaagaa aaatattagt aaaaactttg
     2401 cgttatttta ttgtagcagt gtgcaaatga attgaaaaaa caccccaaat gattcgatat
     2461 ttaaggcata atgaaattga taaacccaag tgggatgatt gcatacaaaa tgcaacgtac
     2521 tccattgtat atgcatactc gtggtacctc gatattgtga gccccaattg ggacgcattg
     2581 gtagaggatg aatacaacac tatttttcca ctcacttgga aaaaaaaata tggaatgatg
     2641 tacctcacgc agcctttatt tgcgcagcaa ttgggtttgt tttcaacgga ggaaatttct
     2701 acaaaaaaaa ttgccatgtt cttaaaaaag ataccccata aatttattca ttgggatata
     2761 agtttcaatc cttcaaataa tgctttgaac agctacattg tttattcaat taaaaggctc
     2821 acttatcata tttcactaag gcaaagttat gatgaaattc accataaata ttctcacgat
     2881 actaaacgtg ttttaaaatt aaatcatcaa catgatttcg taatttcaaa tatactttgc
     2941 gaagaagtat taaaactata caagcaaaac gtgtggcata aagtgccggt agttacattg
     3001 gataatttcg aaacattaaa gcgtttggta aatgaacttt gcaataaaaa tatttgtaag
     3061 gtgcagggta tttttgaaca agataaaatt gctgggagtg cagtacttat aaattgcgac
     3121 aataaattaa tttatctttt tggtgcggca aatgaatcag ggcgtaggag tggggctatg
     3181 cgctattatt ttgacaacat gataaaacaa aattgcaata aaaatttaat tctcgatttc
     3241 gaaggttcga tatttcctac ggtggcgcat ttttataaaa gctttggttc caataaaatc
     3301 gaatttttta atttgcgttt cagcaggata ccaatattaa acctgatgat taaattcaaa
     3361 caaggtataa aacaaaaatt gagtgcatat agaattatgc actcaacttc ggtatgatga
     3421 aaattattgt tttatcattc tttcagattt taatatggca cctttatcag atacttgata
     3481 atagtatact ccattctgta gcatattcaa ttccaatttt aaaaacggtt gtgaaaataa
     3541 atgtgagcat acaacctggc ctttagcatc aaatattttt aattcttttg tagatgatgc
     3601 atcataatat gttgagaaat ttaccttatc gctaaatgga tttggatatg catacacagc
     3661 agaattgcta aaagtaaaat cgttgatacc acttgcccac actccggttt taattaccaa
     3721 tgcttcatat gtaccagcag ttatattcga ctgtccgcca atgactaaac cgccatcgct
     3781 gcatacaact acagtgcgtg cttcgtcttt aatatttttg gtgccgtaca aatgcggtgc
     3841 agtaaagttt cctaaataat ccatctttac taccattata tcaccttgtt catcaccaaa
     3901 attgcacgtt tctccaacac aataaatact accacttgcc agaatgcatg catcagtaaa
     3961 agcatcatat aattttgaac caaattcttt ttcccaaatt acatcaccat tcaaattcaa
     4021 acatgccaca taaccttgca taccattatc ggcagaatat tttcttccta tgcaatataa
     4081 attgtgttgt ggcgattcga gtatactata aatgccatta ctttcgagtg aattgtaatt
     4141 gttgtttgtc cataaaatat ttccattcaa atcacacttt atcagtgtca tactgtattc
     4201 catagtactg ttgttgtaag atgaaccacc aagtatgagc gaattatcgg aagtgcgaat
     4261 catacatttt gcgaaatctc ttttttcggt tccaattgtt tttacaaaaa tttcatttcc
     4321 atttttatcc attcttgcca acataaaatc gagatttgtt gccccataat tctcagtatt
     4381 tcctaatgta ataaaatttc catcagggat ttcgatagaa gtaattgcaa tgtcatcata
     4441 aaatcctcca agttgtttcg accaaatagt attgcccgtt acatcacctc gaatcagata
     4501 catatcacat tcatcgccca agttattggt atgccccgat acaatgaagc caccatcatt
     4561 gcacaaaata atatcgttgc caccatcttc ttttggtaaa ccataatatt ttttccattg
     4621 cgaatttcca ttggcatcaa tcttcataac gtacatatca ccaacggtat cggtcgaagt
     4681 tttgtctaaa ccagcaacca aaaacccgca atcgggcgtt tgtgcaatgc ctcgcacatc
     4741 ggcatacaaa ccagggaaat atgtttttgt aaaatttatt tgttgggcat tggcaaatgt
     4801 tgccgtaatt aaaaacagga tgtaaagctt tttcatttta ttttttttaa tgaaattaag
     4861 taacttttga taagaactat tatccttaga ga
//
LOCUS       DAORQA010000128         4867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4340_length_4867_cov_7.690565, whole genome shotgun sequence.
ACCESSION   DAORQA010000128 DAORQA010000000
VERSION     DAORQA010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4340_length_4867_cov_7.690565"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1944)
                     /locus_tag="PK736_06805"
     CDS             complement(214..1944)
                     /locus_tag="PK736_06805"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC33136.1"
                     /translation="MSIFTNVLVAQNENQKQSTNFMQGYVMSQTTQQPIAYATIYNHT
                     TRMGTVSNSEGYFKIRINDYKDSIAVTMLGYRNYHIGLTPNNNFVSVMLRDNPLLIPE
                     ITVTAQDELYLYEAVAACESNQTKNRFIAKTYFELETYAGNKQIELIESYYHAKVHGY
                     DIEELNLKCGRIGLQKYNDRFFVSVESSQTVNILRLFEQSNYFPQSPMGLGLKKLQKN
                     YNLYLTKKYKDVSDHTILVIEYLPKDTVGNFFTGEVWVDSAQHKVLKITMKTAEAKTH
                     PFLPLHSDDALKEVSMDITKTFANYKGSMVLNHIDFNYQINYKPANEASFMVSTKAIL
                     YAYDYDDKFILPRFNFSANNISDYSKLNALPYNSAFWNNNTEFCLNDLKSRNQIFFND
                     PATITSKRIFAPNETFKKGLLEYPYITWNGSRISFHEFEGQQKQFQDQSKSLAMDMYD
                     LNVKLFVDVNTIADTVNIMTAAVFDPYKSYYKFPVTNASLCFINIYFDLVEIERRELD
                     AELQKSDRSDIAVQALYDSRMAKIEAMKLKYFRETERGNNQAGITKWNQVVVDKIGIN
                     NIVVFNPFGK"
     gene            2220..3215
                     /gene="trpS"
                     /locus_tag="PK736_06810"
     CDS             2220..3215
                     /gene="trpS"
                     /locus_tag="PK736_06810"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRC33137.1"
                     /translation="METVVSGIRSTGNLHLGNYFGAIKNFLRMQDENKCYFFVADYHS
                     LTTHPTPENLMNGVRQVIIEYLACGLDPEKCTLYVQSDVPQVTELYLLLNMNAYVGEL
                     ERCTSFKEKIRKHPENINAGLLTYPTLMAADILLHRAHKVPVGKDQEQHLEMTRTFGN
                     RFNRMYNTEYFAEPTAFNFGTQLVKVPGLDGSGKMGKSEGEGNAIFLNDIEQVITKKI
                     MRAVTDSGPTEPNQPKPEAIQNIFTIMKLVSKADTLEHFENAYNNCTIRYGDMKKQLA
                     QDMNHFVAPFRERIWELQSNNAYIRSVIKMGKEKALESATKTVKEVREIVGFREI"
     gene            3260..4591
                     /locus_tag="PK736_06815"
     CDS             3260..4591
                     /locus_tag="PK736_06815"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33138.1"
                     /translation="MKKIYSVLAMCVWGATASFAQWVPQSSNLPLGNTPDYIDAIDNM
                     ICWATSTNDGGQTVPSDQFCRTTDGGTTWVAGTIADSNSSVAGLVSSCITAVDANNAW
                     VSMWNTAGGTGKIMHTADGGATWTHQATAAYAGAAAFPNFVHFWDLNIGVTMGDPNGG
                     YFEIYTTVDGGTTWTRVPTANIPAELNGEFGIVNVFTTQGASTIWFGTNVGRIYKSTD
                     YGVTWTVATTPYAGIYIGSIAFKDANNGIAFSADQTSVDDVISTNDGGATWTTIANAT
                     TGGLTFRNAACYVPGTPGTYIACSAGQTGGSLFSNDDGNTWNVIDADPHTDVEFTSDI
                     DGFGGTLTQDGGSIYQIYKWTGPVGISEIANTTNANVFPNPATDKIFVKTNNATQYEI
                     VNVTGQSIAKQQIVYTGANLTIDVRNLQSGIYFLRLIQNDGTLSTNKFVKQ"
BASE COUNT         1507 a          923 c          894 g         1543 t
ORIGIN      
        1 tatataaata ttttttgtca attccaccaa ctatttttga gctcattacc aaattttata
       61 aaaaatctat aattcttgtt taggcaaatt aaaaaactgg gcttaagccc aatacttata
      121 ttgtatgtat ccccatcata aatgatggag ttaatcacaa aatgtttcaa aaccttacaa
      181 tgaaaaacac tacaaaactt ttggcaaaaa aatctacttg ccaaacggat taaaaacaac
      241 aatgttatta atgccgattt tatctaccac tacttgattc cattttgtaa tgccggcttg
      301 gttatttccg cgttctgttt ctctaaaata ttttaatttc atggcttcta tttttgccat
      361 tcggctatcg tacagtgcct gcacagcaat atcgctgcgg tcgctctttt gcaattcggc
      421 atccaactcg cgtctttcaa tttcaaccaa atcgaaataa atatttataa aacataacga
      481 agcattggta acaggaaatt tataataact cttgtaagga tcgaacactg cggcagtcat
      541 aatatttact gtgtcggcaa tggtgtttac atccacaaat aattttacat tgaggtcgta
      601 catatccatt gcaagcgatt tgctttgatc ctgaaattgt ttttgctggc cttcgaattc
      661 gtgaaaactt attcgcgatc cattccaggt tatgtatgga tattcgagca accctttttt
      721 gaaagtttcg ttgggcgcaa atattctttt acttgtgatg gttgccgggt cgttaaaaaa
      781 tatttgattc cggcttttta aatcattcaa acaaaattct gtattgttat tccaaaaagc
      841 ggagttgtaa ggcagtgcat ttagtttgct ataatccgaa atattatttg cactaaaatt
      901 aaaacgtggc agaatgaatt tatcatcgta atcataagca tataatattg cttttgtcga
      961 caccataaaa cttgcctcgt ttgcaggttt gtaattgatt tgataattaa aatcgatatg
     1021 atttaaaacc atgctgcctt tgtagttggc aaatgttttt gtaatatcca tactcacttc
     1081 ttttaatgca tcgtcactgt gtaacggtaa aaacggatgc gtttttgctt cggccgtttt
     1141 cattgttatt ttcaacactt tatgttgtgc tgaatcgacc caaacttcgc cagtaaaaaa
     1201 attacccaca gtgtcttttg gcaaatattc tatcactaaa attgtatggt cgcttacatc
     1261 tttatacttt tttgttaagt atagattata atttttctgc aactttttta atcccaagcc
     1321 cattgggctt tgaggaaaat aattgctttg ctcaaaaaga cgcaatatgt ttacagtttg
     1381 cgaactttcg accgaaacaa aaaagcgatc gttatatttt tgaagtccta ttcgcccaca
     1441 cttaagattc aattcttcaa tatcgtagcc atgtacttta gcatgataat aactttctat
     1501 taattctatt tgcttgttgc ctgcataagt ctcaagctcg aaatatgttt ttgctataaa
     1561 ccggtttttg gtttgattac tttcacaagc agctacagct tcgtacaaat ataattcatc
     1621 ttgggctgta acagtaattt cgggaatcaa cagtggatta tcgcgtaaca ttaccgatac
     1681 aaaattatta tttggtgtaa gtccaatgtg ataatttctg tatcccaaca tggttactgc
     1741 aatagaatct ttgtaatcat taattctgat tttaaaataa ccctcactat tacttactgt
     1801 gcccatgcgg gttgtatggt tgtatatagt agcataagca atgggctgtt gtgtagtttg
     1861 gctcattaca tacccctgca taaaatttgt gctttgtttc tggttttcgt tttgtgcaac
     1921 taatacattg gtaaatattg acatacaaaa catgcttaca agcaatgcta taatgaatgt
     1981 gacattgtta tatagaagtt tcaaaggcct catgtgggaa tactgatacg ttacttttct
     2041 tcggtgtgcg aatataacat ggattattca atgctacata cttattatca ggcaatacca
     2101 acctgatgat gtgtaaaaaa aaccgaaacc atttttcttc taaaacattg ctaccactgg
     2161 aatttcgctt tcctaaaaaa taattgccct aacatatttt ataattttac ttttgcaaaa
     2221 tggaaacagt agttagcgga atacgcagca ccggcaattt acatttggga aattattttg
     2281 gtgccataaa aaatttcctg cgcatgcagg acgaaaataa gtgttacttt tttgtggccg
     2341 attatcattc gctcaccaca caccccacgc ctgagaacct gatgaatggt gtgcggcagg
     2401 tgataataga atatttagct tgtggcctcg atcccgaaaa atgtacctta tatgtgcaaa
     2461 gcgatgtacc tcaggttact gaattatatt tgctgctaaa catgaatgct tatgtaggcg
     2521 aattggaacg ttgcacttct tttaaagaaa aaattcgaaa gcatcccgaa aatataaatg
     2581 ccggattgct tacctatcct actttgatgg ctgccgatat cttattgcac cgtgctcaca
     2641 aagtgcctgt gggtaaagat caggaacaac accttgaaat gacacgcacg tttggcaatc
     2701 gatttaaccg catgtacaat accgaatact ttgccgagcc tactgctttt aatttcggca
     2761 cacagttggt aaaggtgccg ggcctcgatg gaagtggaaa gatgggaaaa tcggaaggcg
     2821 agggcaatgc cattttttta aatgacatag aacaagtaat tacaaaaaaa ataatgcgtg
     2881 ctgtaaccga tagtggccca accgaaccca atcaaccaaa gcccgaagca atacaaaaca
     2941 tttttaccat tatgaaactg gtatcgaaag ccgatacact ggaacatttt gaaaatgctt
     3001 ataacaattg caccattagg tatggcgaca tgaaaaagca acttgcacaa gacatgaacc
     3061 actttgttgc gccatttcgt gagcgcatat gggagctaca aagtaacaat gcctatatac
     3121 gtagtgtaat taaaatgggg aaagaaaaag cgctggaaag tgcaacaaaa actgttaaag
     3181 aagtacgcga aatagttggg ttccgcgaaa tttaattatt ttcgcgccca atatttaaaa
     3241 acaaaaaatt tataaaaaga tgaaaaaaat ttactctgtt cttgcaatgt gtgtttgggg
     3301 tgctacggca tcgtttgcac agtgggtgcc acaatcatcc aatttacctt tgggcaacac
     3361 gcccgattat attgatgcta tcgacaacat gatttgttgg gcaacatcaa ccaacgatgg
     3421 cggtcaaacc gttccatcag accaattttg ccgcactacc gatggcggta ctacatgggt
     3481 tgcaggcact attgccgaca gcaactcgag tgtggccggt ttggtttctt cttgtattac
     3541 ggctgtagat gccaacaatg cgtgggtatc gatgtggaat actgccggtg gtacaggaaa
     3601 aattatgcat accgctgatg gtggtgctac atggactcat caagctacgg cagcatatgc
     3661 cggtgctgca gccttcccaa actttgtaca cttttgggat cttaatattg gtgttaccat
     3721 gggcgatccc aatggtggtt acttcgaaat ttataccacc gtggatggcg gtacaacctg
     3781 gacacgtgta ccaacggcaa atattcctgc tgagttaaat ggtgagtttg gaattgtaaa
     3841 tgtatttaca acccaaggcg ctagcactat ttggtttggc accaatgttg gccgcattta
     3901 caaaagcacc gactatggcg taacctggac agttgcaacc acaccttatg caggtattta
     3961 tattggcagt attgctttta aagatgcaaa taatggtatc gctttctcgg ccgaccaaac
     4021 tagtgtagat gatgttattt ctactaatga tggtggtgct acctggacta ccattgcaaa
     4081 tgctactacg ggtggattaa catttcgtaa tgccgcttgc tacgtacccg gcacacccgg
     4141 cacttacatt gcctgcagtg cagggcaaac aggcggaagt ttattttcga atgacgatgg
     4201 caatacctgg aatgtaatag atgctgaccc acataccgat gtagaattta ccagcgacat
     4261 tgatggtttt ggtggtacac ttacacaaga tggcggttct atctatcaaa tttataaatg
     4321 gacaggccct gtaggaatca gcgaaatagc gaacaccacg aatgcaaatg tgtttcctaa
     4381 tcctgcaacc gacaaaatat ttgtaaaaac aaataatgcc acacaatacg aaatagtaaa
     4441 tgttacaggc caatcaattg ccaagcagca aattgtatac accggtgcta accttaccat
     4501 cgatgtaaga aatttacaaa gcggtattta ctttttacgt ttaatacaaa acgatggtac
     4561 attatctacc aacaaatttg tgaaacaata attttaatta tcactgaata atataaaaag
     4621 gctgtctgta atgggcagcc ttttttatgg gttagaaaat caattaccat ttatgaaaaa
     4681 tgtttttttc taaatatgta aagtattaaa cggaagcatc ataacattag aatttgtaag
     4741 attgctccaa tattttttct tgcacacatc attttccttt atacatttac aacacaaaat
     4801 acattgtgct ttgagtctcc agtcgaaatt ttaattgcac attttttaat cagaattatt
     4861 tatgagt
//
LOCUS       DAORQA010000129         4798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4421_length_4798_cov_9.401855, whole genome shotgun sequence.
ACCESSION   DAORQA010000129 DAORQA010000000
VERSION     DAORQA010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4421_length_4798_cov_9.401855"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PK736_06820"
     CDS             <1..277
                     /locus_tag="PK736_06820"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRC33139.1"
                     /translation="GLFDELNKQSMTVRDVNLQKFANLDSPIEAITKIRYKDPGTNST
                     IAMEGTGMKVTFHKPIHAVAPGQSAVFYDGDDVIGGGFIEKEINRDS"
     gene            647..1102
                     /locus_tag="PK736_06825"
     CDS             647..1102
                     /locus_tag="PK736_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC33140.1"
                     /translation="MLPLSEKYKVIEPLTEEEFDQYFLLRWKVLRKPWNQPPGSEVDF
                     NEATSMHGLILNSHGKAIAVCRIEMRDVLTAQVRYMAVSEEVRGQGLGRKILHYLEKK
                     AKNKGARRVYLDARENAVDFYVSCGYRNIKTSYLLFGEIQHYGMEKSLG"
     assembly_gap    1289..1300
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1301..>1508
                     /locus_tag="PK736_06830"
     CDS             <1301..>1508
                     /locus_tag="PK736_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRC33141.1"
                     /translation="KAGDGLTPIDIVKFTTGYGRWLLTTVSNGTKCKVVVGRDARISG
                     QMVQSIVTSTLSALGIDVVCLQLST"
     assembly_gap    1509..1518
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1519..2662
                     /locus_tag="PK736_06835"
     CDS             <1519..2662
                     /locus_tag="PK736_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRC33142.1"
                     /translation="PTVELAVVWHNANGGIIITASHNPKQWNALKLLNSSGEFISATD
                     GEMVLQFAEDGQTNYAEVTKLGKYEEDNGAIEKHIACILELPMVDVEAIRSKNFRIVV
                     DAVNSVGGIAVPMLLKSLGVEDIIELYCEPTGNFPHNPEPLPENLVDLAQMVKRKDAH
                     LGIVVDPDVDRLAFVCEDGEMFGEEYTLVAVADFVLKNKKGNTVSNLSSTRALRDVTE
                     KAGLQYFASAVGEVNVVEMMKQQNAVIGGEGNGGIILPELHYGRDALVGIALFLTHLA
                     KFGKSASVLRHQYPDYHISKNKIELTPGIDVDAILAAIQEKYKKHPTSTIDGVKLEFG
                     MEWVHLRKSNTEPIIRIYAESQSMTMSNNLASKIMSDIKEIVQNKK"
     gene            2681..3808
                     /locus_tag="PK736_06840"
     CDS             2681..3808
                     /locus_tag="PK736_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRC33143.1"
                     /translation="MKVYFDNAATTPMLVEVIESMTTVMQNQFGNPSSIHSFGRETKS
                     LIEKSRKSIATLLNCSPGEIFFTSGGTEANNMAIRCSIEQLGITHAISSPIEHHAVLH
                     TLEDLHHNNKVRLSLVKIDNKGVVDLNHLEELLKSNPRTFVSLMHANNEVGTVLPLVE
                     VGNICAKYDALFHSDTVQTMGHLPHNLGETNVQFINCAAHKFHGPKGVGFIYINSKVK
                     IKPFITGGAQERNMRGGTENLYGIIGLAKALEVSYTHMTEDKEKVQGLKNYMIDQLQK
                     NIPDVQFNGDISDDSLYTVLNVSLPPTPIAEMMLFNLDIRGIAASGGSACSSGSEVGS
                     HVLTALGTNPDRPAVRFSFSRFNTKDEVDYAITQLVELYKM"
     gene            3848..4468
                     /locus_tag="PK736_06845"
     CDS             3848..4468
                     /locus_tag="PK736_06845"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRC33144.1"
                     /translation="MLLEINLENIDNRKISQVIDVLKKGGLVIIPTDTIYAACCSMQS
                     KKGFQRLCEFKNIAPAKASFSMLLSDLSSISHYTKQFDRNIFRILKKALPGPYTFILP
                     CSQAVCEVFSSAKKTIGVRVPENDFVQLLIKELDSPLLAASLHDEDEIIKYSTDPSTI
                     YEQFDEKVDLIVDGGYGKNTGSTVVDCSDGSPLVLRQGLGGIDILD"
BASE COUNT         1570 a          814 c          938 g         1454 t
ORIGIN      
        1 gggcttgttt gatgaactca ataagcaatc gatgacagtt cgggatgtta accttcaaaa
       61 gtttgccaat cttgatagcc caatcgaagc cattacaaaa atcagataca aggatcctgg
      121 aacaaatagt acaattgcca tggaaggaac cggaatgaaa gttacgtttc acaaacctat
      181 tcacgcagtt gctcctggtc aaagtgctgt tttttacgat ggtgacgatg tgataggcgg
      241 aggctttatt gaaaaggaaa taaacagaga ttcttaatga tttttaacat ttgattttga
      301 ttaatccaaa tcataacaaa tgtctaatct tgtttttctt gcgctgattg gattagttgt
      361 aaaacatttt tgtgtatgat taatcgattc ggttaagtaa aagcaagact aaattgaatg
      421 ggagaagcaa ggtgtttact gagttgcgat tccaaactta aggatatagt aaaaaatgtg
      481 taacattact aatttaatat aagtatctta tttacaattg tttatgatag tattttaaat
      541 tataatttta tattcttatt ttagacttaa aaaaaaatat cataaaagtt aaaataattt
      601 tgcacatata aatttacaaa ctacattagc caatcaaaaa aaagctatgt taccactttc
      661 cgaaaaatac aaagtgatag agcctcttac tgaagaggag tttgatcagt attttttatt
      721 gcgttggaaa gtattgcgta aaccctggaa tcagcctccc ggcagcgaag tggattttaa
      781 cgaagcaaca tctatgcatg gtctcatatt gaattctcac ggtaaagcaa ttgctgtttg
      841 ccgtatcgaa atgcgtgatg tacttacagc acaggttcgc tatatggctg taagtgaaga
      901 agtaagagga caagggcttg gccgaaaaat attacattac ctcgaaaaga aagccaagaa
      961 caaaggcgct cgaagggttt acctcgatgc ccgcgaaaat gctgttgatt tttatgtttc
     1021 atgcggatac agaaacatca aaacatctta tcttctattt ggtgaaattc aacattacgg
     1081 tatggaaaaa agcctgggtt aattttccgc tttcgactgt tcatattcaa cacatctcat
     1141 tttaaaaatt cccattcgta tttatattga aaacttatcc caacaagttt cgaatactaa
     1201 cgtttgcaat tctatatttg cgcacttttt caatcattaa tttaaaatcg ctttgtcatt
     1261 aattaaatct atatcaggaa tacgaggann nnnnnnnnnn gtaaagccgg agatggccta
     1321 acacctatag atattgtaaa attcacaaca ggttatggaa gatggcttct taccacagtt
     1381 tcaaatggaa caaaatgcaa agttgttgtt ggtcgtgatg cgcgcatctc ggggcaaatg
     1441 gtccagagca ttgttacttc aacactttcc gctttgggga ttgatgtggt ttgtcttcag
     1501 ttatcaacnn nnnnnnnngc ctacagttga gttggcagta gtttggcaca atgcaaatgg
     1561 tgggattatt atcaccgcaa gccacaaccc caaacagtgg aatgctttaa aactactaaa
     1621 cagttcaggg gaatttatat cggccacaga tggcgaaatg gttttgcaat ttgctgaaga
     1681 cggtcaaaca aactatgccg aagtaaccaa gttaggtaag tacgaagagg acaatggagc
     1741 aattgaaaag catatcgctt gtattttaga attgccaatg gttgatgtgg aagctattcg
     1801 cagcaaaaat tttcgaattg tggtagatgc tgtaaattct gttgggggca ttgcagtgcc
     1861 tatgcttttg aagtcgcttg gtgtagaaga tattattgaa ttgtattgtg aaccaacggg
     1921 taacttcccg cacaatcctg aacctttgcc cgaaaacctt gtagaccttg cgcaaatggt
     1981 aaagcgcaaa gacgctcacc ttggaattgt tgttgacccc gatgttgaca gacttgcatt
     2041 tgtgtgtgaa gatggagaga tgtttggcga agagtatacc ttggttgccg ttgccgattt
     2101 tgtattgaaa aataagaaag gcaatacagt ttcaaacctt tcatctacgc gtgctttgcg
     2161 cgatgttact gaaaaggcag gacttcaata ttttgcatcg gcagttggcg aagtaaacgt
     2221 agtagaaatg atgaagcaac aaaatgcggt gataggaggt gaaggaaatg gaggtattat
     2281 tttacctgag ttgcattatg ggcgcgatgc attggttggt attgcattat tcctcacaca
     2341 tttggcaaaa tttggaaaat cagcatctgt attgcgtcac caatatcctg attatcatat
     2401 ttcgaaaaac aaaatagaat taactccggg tatagatgtt gatgccatcc ttgcggccat
     2461 tcaggaaaaa tataaaaaac atcctacttc aactatcgat ggtgttaaat tggaatttgg
     2521 aatggaatgg gtacacttac gtaaatccaa taccgagccc ataattagaa tttatgctga
     2581 gagccaaagt atgactatgt caaataatct ggcatcgaaa attatgagtg atatcaagga
     2641 gattgtacaa aataagaaat aacaaattat tcaaaacaaa atgaaagtat attttgataa
     2701 tgccgctaca acacctatgc ttgttgaggt tatagaaagc atgacgaccg ttatgcaaaa
     2761 ccaatttgga aatccatcat ctatacattc atttggacgg gaaacaaaat ctctgattga
     2821 aaaatcgcga aaatctattg ctactttatt gaattgttct cccggtgaaa tattttttac
     2881 ttcaggtgga accgaagcca acaacatggc aatacgttgt agcattgagc aactgggaat
     2941 cacccacgcc atttcatctc ctattgaaca ccatgctgta ttacacactt tagaagattt
     3001 acaccataac aataaagtta gactcagtct tgtaaaaata gacaacaagg gcgttgtaga
     3061 tttaaatcat ttagaggaat tactaaaaag caatcctcga acttttgtat cattaatgca
     3121 cgccaataat gaagttggta ctgtgttgcc tcttgttgaa gttggaaaca tttgcgcaaa
     3181 atatgatgcg cttttccatt cagatacagt gcaaacaatg gggcatttgc ctcataactt
     3241 aggggaaact aatgtccaat ttattaattg tgccgcacac aaatttcatg gccccaaagg
     3301 agtaggattt atttacatca acagcaaagt gaaaataaag ccctttataa caggcggtgc
     3361 acaagagcga aatatgcgtg gcggtaccga aaacctatac ggaattatag gccttgcgaa
     3421 ggcactcgaa gtttcatata cgcacatgac tgaagataaa gaaaaagtgc agggccttaa
     3481 aaattatatg attgatcaat tgcaaaaaaa tattcctgat gttcagttta atggcgacat
     3541 atctgacgac agcttatata ctgtgcttaa tgtaagttta cctccaacac ctattgccga
     3601 gatgatgttg ttcaaccttg atattcgtgg tattgctgct tcgggtggta gtgcatgttc
     3661 atcgggtagc gaagtaggtt cacatgtact tacagcatta ggcactaatc ccgatcggcc
     3721 agcggtacgg ttttcttttt caaggtttaa taccaaagac gaagtagatt acgcaattac
     3781 acaattggta gagctatata aaatgtaatt gagtttgtaa atttatatct caaccagttt
     3841 ttcaattatg ctgctcgaaa tcaatttaga gaacatcgat aacagaaaga tatcacaagt
     3901 aattgatgtt ttaaaaaagg gcgggcttgt aattattcct accgatacta tttatgcggc
     3961 ttgttgttca atgcagagta aaaaagggtt ccagcggtta tgcgagttta aaaacattgc
     4021 tccggccaaa gcatcttttt caatgctact tagcgattta tcttccatat cacattacac
     4081 caaacaattt gatcgcaata tctttcgcat cttaaaaaaa gctttgccgg gtccttatac
     4141 atttattttg ccatgctctc aggcagtatg tgaggtattt tcttcagcaa aaaagacaat
     4201 aggagtaaga gtgcccgaaa acgattttgt acagttgcta attaaagaat tggattctcc
     4261 attacttgct gcttcgcttc acgatgaaga tgaaatcata aaatattcta cagacccgtc
     4321 aaccatttac gagcagtttg acgaaaaagt cgacctgatt gttgatggtg gctatggaaa
     4381 aaatacaggc agtacagttg tagattgtag tgatggttct cctttagttt taaggcaagg
     4441 acttggtgga attgatatac tcgattaaag aacaaagtga tttaacactg aagaatgaat
     4501 gcagaaattt ataattgcaa tcacttaata aaaataaaat acttttgaag ccatgaacta
     4561 ttctatactt gaacagagca aatttgaacg gcttttgaag tcagtatttt cagttttatt
     4621 ttttgttgtt ttctcatcac tccacgctca acccttatat aaggaatata ttgaaagaga
     4681 atatcctgag gatgtttacc acactcaaat atcaaagtca accctaaaaa atgttgaaat
     4741 taagacaatc tgtaatattc ttaatctcgg ggaaaagttt tattttactt tcttcttt
//
LOCUS       DAORQA010000130         4786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4431_length_4786_cov_9.252167, whole genome shotgun sequence.
ACCESSION   DAORQA010000130 DAORQA010000000
VERSION     DAORQA010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4431_length_4786_cov_9.252167"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PK736_06850"
     CDS             <1..431
                     /locus_tag="PK736_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33145.1"
                     /translation="LGLAYSYKWKEARNKSAENKFADSAKTNLLHNILVQQKNEKYKS
                     SGVALALSTVIPGLGKVYAGRWKDGIISLLFVGGMGIQAYRAFDQRGVKSTRGWIFAT
                     MGTGFYLGNLYGSYHAARIRNNKINEKYLLQINNSLDSLY"
     gene            382..1233
                     /locus_tag="PK736_06855"
     CDS             382..1233
                     /locus_tag="PK736_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33146.1"
                     /translation="MKNIFCKSIIAWIVCTSFLIRANGQSIDETFTLALQQYAEGNYD
                     YASELAQRVTFFDTDYKYIPGCDLLVAKCYMQKKEFSLAANFFEKAFSNNTNDSIRTE
                     CLFDKASCHLLQREYNFALTELLTIRDKPSQPNTTKKKNLFLAVTYYYLNEFELSKLN
                     MLAIDALDSLETKKIDQLYLQNDKLTKRYKPKTARIMSLIIPGSGQMYAGDYKNGINS
                     LLLVAAFSYLGYTVVVNGTLGDAIISILPWLQRYYYGSSQKAEIICRDKLQYERNKLI
                     QNYLKQL"
     gene            complement(1261..2190)
                     /locus_tag="PK736_06860"
     CDS             complement(1261..2190)
                     /locus_tag="PK736_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33147.1"
                     /translation="MQPNINFGNDTTLCNGDSLVLNVNGTFNSYTWQNGSTNSSFVVN
                     APGTYYVSAVNTCDTKTDTIVVAYTACGNVPAALFSSSDTLWCDKTCIDFYDLSQNTP
                     TTWQWYFAGASPTTSTDQNPTSICYNNYGSFDVVLVVCNGSGCDSVYKQNFVTEFQLP
                     AVPTVTLSGDTLYSSPATSYQWFNVNNVNLVLGTNNYFVPTVAGSYYVLISDSNGCQT
                     PSAVISIALSVPTIHLNNNYIVYDNANHLLQLKNINTNMPYVIHSADGKICLQGNTNL
                     HNQQIDVSSLARGIYTITIYDGQLPLTTKVAIY"
     gene            complement(2209..2877)
                     /locus_tag="PK736_06865"
     CDS             complement(2209..2877)
                     /locus_tag="PK736_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33148.1"
                     /translation="MKKVLLAVYFVFMCSFCNALNVKIIESQSYNVGHVMDTVWLNLI
                     SSLGHTATIEPQILLNSNAFFASTDVLIISSGVITLPANYVTTIQQFLQAGKPVYIQA
                     EYLKSYSTTAAFESLVNILGGTFVWGTELSGDLAPVIPQSTFASTPNSVASINYYWYG
                     VEANTTPTVSAVIKNQNFNLGFFFCPPTPCTGKLITTTDQDWIRAADPADLQLMENLL
                     FMQN"
     gene            complement(2885..>4786)
                     /locus_tag="PK736_06870"
     CDS             complement(2885..>4786)
                     /locus_tag="PK736_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33149.1"
                     /translation="YSKVDLKLNGGLGDVTAKNVSVVPGALGEKVTACYHANGVDFWV
                     TVHGLNNSNYYTALVTAAGVQPAVMQTIGAGMTGIWTGGMSFNAQSTKCAMTTYDMHT
                     MELIDFDNSTGVFSNPVSKTFPTSYYPYSTEFSASGNYLYCCNVDYTPGEIYQFDMNA
                     GSNAAIIASGISVHTFTDIPGDIQLALDGKIYFARYSTQWVGVINSPDSAGTACNAVN
                     NAVNVSPGTCALGLPDFVTNILGTNIQAQDLCLGDSTHFSLFNGGNIIAIQWNFGDPN
                     SGANNTAYIANPAHQFTDSGTYNVQLILVYLNLTSDTINTIVNIGIQPVVALGNDTTL
                     CIGQSLVLNAGPGKNYLWQDGSTNSTYTATVNGTYGVQVTNNSGCKGVDSINVDFIQC
                     AGIVAALSSSDTLWCDKTCIDFTDLSLNNPTSWTWYFQGASPSTSTDQNPTGICYNNY
                     GSFDVTLVACNAIACDSLFLDDFVTEFQLPAAPTISFSNDTLYSSPAFSYQWYNTNNI
                     NVVLSTNNYFVPVVDGNYFVLAFDSNGCSVPSPTFGFYTSFQNLTGSSGISVFPNPAS
                     NELTITLSPSISENVLVELYDITGRIVVSKTINEMSNTINLQNVNAGTYQLKFITEKG
                     AYVKVISKM"
BASE COUNT         1470 a          946 c          953 g         1417 t
ORIGIN      
        1 cacttgggtt ggcttatagt tataaatgga aagaagcccg aaataaaagt gcagaaaaca
       61 aatttgccga cagtgcaaaa actaatttac tccacaacat tttagtacaa caaaaaaacg
      121 aaaagtataa aagctccggt gtggcacttg cattatcaac tgtaattccg ggcttgggca
      181 aagtgtatgc cggtcggtgg aaagatggaa ttatttcgct gttatttgtt ggtggcatgg
      241 gcatacaggc ttaccgtgca tttgatcagc gtggcgtaaa aagcacgcgg ggatggattt
      301 tcgccactat gggtaccgga ttttatcttg gcaatttata tggctcgtac catgctgccc
      361 gtatacgcaa caataaaata aatgaaaaat atcttttgca aatcaataat agcctggata
      421 gtttgtacta gctttttaat aagggccaat ggccaaagca tagatgaaac attcaccttg
      481 gcattgcaac aatatgccga aggtaattat gattatgcaa gtgagcttgc tcagcgtgtt
      541 acgtttttcg atacggatta caaatacatt cccggttgcg atttattggt ggccaagtgt
      601 tacatgcaaa aaaaagaatt ttcgctggcg gcaaattttt tcgaaaaagc tttcagtaat
      661 aataccaacg acagcattcg cacggagtgt ttatttgaca aagcatcgtg ccacttgctt
      721 caacgcgaat acaattttgc ccttaccgaa ttattgacaa ttcgcgacaa gccttcgcaa
      781 cctaatacta caaaaaagaa aaatttattt ttggctgtta cctattatta cctcaacgaa
      841 tttgagcttt ctaaattaaa catgctggcc attgatgcat tagatagttt ggaaacaaaa
      901 aaaattgacc agctatattt acagaacgac aaacttacaa agcggtacaa accaaaaaca
      961 gcccgtatta tgagtttgat aattcccggc agtggacaga tgtatgcagg tgattataaa
     1021 aatggcataa actccctttt gcttgtagct gctttttctt atcttggata cactgttgta
     1081 gtaaatggta ccttaggcga tgccattatt tctatactgc cttggttaca gcggtattac
     1141 tatggctcat cgcaaaaggc agaaataatt tgccgcgata aattgcagta tgaaagaaac
     1201 aaactcatac aaaactatct caagcaactt tagtttggat attctccgaa aggaaacttt
     1261 ttaatagata gccacctttg ttgtcagtgg cagttgacca tcgtaaatgg ttatagtata
     1321 aatacctcgt gcaagtgacg atacatctat ttgttgattg tgcaaattgg tattaccctg
     1381 caggcaaatt tttccatcag cactgtgtat gacataaggc atatttgtgt taatgttttt
     1441 taactgcaat aagtgatttg cattgtcgta aacaatatag ttattgttta aatgaatggt
     1501 tggcacagaa agcgctatgc tgataaccgc agaaggagtt tgacatccgt tactatcgct
     1561 tattaaaaca taataactac cggctactgt tggcacgaaa taattattgg tgcccaacac
     1621 taagtttaca ttgttcacat taaaccattg ataagaagtc gctggcgaag aatacaatgt
     1681 atcgcccgac aaagtcaccg ttggcacggc aggcaattga aactcggtaa caaaattttg
     1741 tttgtatacc gaatcgcacc cactgccatt acacaccacc aacactacat cgaaactgcc
     1801 atagttgtta taacatatgc tggtgggatt ttgatcggtg gatgtagtgg gagatgctcc
     1861 ggcaaaatac cattgccatg ttgtaggagt attttgtgag aggtcgtaaa agtcgatgca
     1921 cgttttatcg caccataatg tatcgctcga tgaaaataat gccgcaggca cattaccaca
     1981 tgctgtataa gcaaccacta ttgtatctgt ttttgtatca catgtgttta ccgcactcac
     2041 ataataagtt ccgggagcat tcaccacaaa cgaagaattc gtgcttccgt tttgccatgt
     2101 atacgaatta aaagttccat tcacattcaa cactaatgaa tcaccattgc ataaagtggt
     2161 atcgttacca aaatttatat ttggctgcac cacaacataa ttctaaaatt agttttgcat
     2221 aaacagcagg ttttccatca gttgcaaatc tgccgggtcg gcagcgcgta tccaatcctg
     2281 atcggtggtg gtaattagct ttcctgtaca aggagttgga gggcaaaaga aaaaacctaa
     2341 attaaagttt tgatttttta taactgctga tacagttggc gttgtattag cttctactcc
     2401 ataccaataa taattaattg acgcaacact gtttggtgtg cttgcaaaag ttgattgcgg
     2461 aatcacgggc gccaaatcgc cacttagttc ggtaccccag acaaatgtac caccaagaat
     2521 atttaccaga ctttcaaaag ctgccgtggt ggaataagat ttcaggtatt cggcctgtat
     2581 gtataccggc ttaccggcct gcaaaaattg ctgaatggtg gttacataat ttgcaggtaa
     2641 tgtaatcacc cccgatgaaa tgataagcac atcggtgctg gcaaaaaacg cattgctgtt
     2701 taatagtatt tgtggttcta tggttgcggt atgaccaagg ctgctgatta aatttaacca
     2761 aacggtatcc attacatgtc caacattata gctttggctc tctataattt ttacattcaa
     2821 ggcattgcaa aaactgcaca tgaatacgaa ataaacagca agtaaaacct ttttcatttt
     2881 gtgattacat tttagaaatg acctttacat atgctccctt ttcagtaatg aattttaact
     2941 gatatgtgcc ggcatttaca ttttgcaagt ttatagtatt agacatttcg tttatggttt
     3001 ttgaaactac tatgcggcct gtgatatcat aaagttcgac caatacgttt tcggaaattg
     3061 atggcgaaag tgttatggtt aattcattgc tggcaggatt tgggaaaaca ctaatgcctg
     3121 aactgccggt aagattttgg aacgaagtat agaaaccaaa agttggtgat ggcaccgaac
     3181 aaccgttgct atcgaaagcc aacacaaaat aattgccatc tactacaggc acaaaataat
     3241 tgtttgtact caacacaaca ttgatattat tggtattgta ccattgatat gaaaatgcag
     3301 gtgatgaata taaagtgtcg ttgctaaatg aaatggttgg ggccgcaggt aactgaaact
     3361 cggtaacaaa atcatcgagg aaaagagaat cgcaggcaat ggcgttgcat gctacaaggg
     3421 taacatcaaa gctgccataa ttattgtaac atattccggt agggttttgg tcggtggatg
     3481 tggaaggcga tgccccctga aaataccatg tccacgatgt cggattattt aacgatagat
     3541 cggtaaagtc tatacaggtt ttatcgcacc atagcgtatc gctcgatgat agtgctgcaa
     3601 ctattcctgc acattgaata aaatctacgt ttatagaatc cactcctttg catccgctgt
     3661 tgtttgtaac ttgtacaccg tatgttccgt taacggtagc agtatacgta ctgttggtac
     3721 ttccatcctg ccacaaataa tttttgcccg gcccggcatt caataccaaa gattgcccta
     3781 tgcatagcgt agtatcgtta ccaagtgcta ccaccggttg aatgccaata tttacaattg
     3841 tattaatggt gtcgcttgta agatttaaat acaccaaaat caactgcaca ttgtaagttc
     3901 ccgagtcggt aaactgatgc gctggattgg ctatatatgc cgtattgttt gctcctgaat
     3961 tggggtcgcc aaaattccat tgtatagcaa tgatgttacc gccattaaac aaactaaaat
     4021 gtgttgaatc gccaaggcat aaatcctgtg cctgtatatt ggtgcctaat atattggtaa
     4081 caaaatcggg caagcccaat gcacatgtac cggggctaac attaacagca ttgttcacag
     4141 cattgcaggc agtacctgcc gaatcgggcg aattaataac tccaacccat tgtgtgctgt
     4201 agcgggcaaa atatatttta ccatcgagtg ccaattgtat atcgcccggt atatcggtaa
     4261 atgtatgtac cgaaattccc gaagctatga tagcagcgtt gctacctgca ttcatatcga
     4321 actgataaat ttcgcccgga gtataatcta cattgcagca atataaataa ttaccacttg
     4381 ccgaaaactc ggtgctgtaa ggataatagc ttgttggaaa agtttttgat accggattcg
     4441 aaaacactcc ggtactgtta tcaaaatcga tcaactccat ggtgtgcata tcataagtgg
     4501 tcatagcgca ttttgtgctt tgtgcattga agctcatacc gcctgtccaa atacccgtca
     4561 taccagcacc tatagtttgc atcactgcgg gttgcactcc tgcggcagtt actaaagccg
     4621 tatagtaatt tgaattattt aagccatgca ctgtcaccca aaaatcgaca ccgtttgcat
     4681 gataacatgc agtaactttt tcgccaagtg caccaggcac taccgaaaca ttttttgctg
     4741 taacatcgcc aagtcctcca tttaatttaa gatctacttt cgaata
//
LOCUS       DAORQA010000131         4749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4474_length_4749_cov_9.763528, whole genome shotgun sequence.
ACCESSION   DAORQA010000131 DAORQA010000000
VERSION     DAORQA010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4474_length_4749_cov_9.763528"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4749
                     /locus_tag="PK736_06875"
     CDS             <1..>4749
                     /locus_tag="PK736_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33150.1"
                     /translation="IEYFQSQRDFPMIDNTPASVTISALNVCAPLMPVLNNVTAYPAH
                     QFALPMTFQQLNTEVKNIEIGVEQYNTHCENLVTFPTPADYAISTAQWINAANALWPI
                     VLPNTHNNYKFAVSGNKPYEFNTERRTKWNEQVYPYLNNTAMLEGKYSIILHPYMGSG
                     LSGLGVNNTTNINEMMVQPFQYYDNFLEKEIHQNIKPYGINHYFNTTKPDIWITEGNM
                     FSKTGVVHGSWAAALFTGTLFLKMMEENQIKILDHHAMAGNFVWADYFNSNDGYITGD
                     FDAPDVDGNTTNGLQVPTTVNLKGSLTLQGHVMRMIGSCFRNAAQAHLLNFTGVNMAV
                     PSYTPIGTTAIYPMLYGWEFAASSATDNQILLLNLTDQPQSIDVSSFNFNAVNTLGYQ
                     LAFPFNSFLSDDNNGYYLHTYSTNSYTSTNSTPIYQQPLTWSNGYIILPAYSITKMQK
                     CINTPPVLVASTTEPCNGLPFSIKVVNNKNYTGNYTWVSSNNASTTTTTEPVLVTNGI
                     TANTTYTVTVNICIGGTTSNSILIKAHTNLTANLTTNTSLPLCITGNAAQLTATLSGS
                     SAFKSYAIYALDGADPAYVAPDVGYGNTNNSMFLTNVHPTATTQYQVTASDGVCAVTS
                     PPIAITVVPKLEITPNVDQVCTGIYTDLAVDNTFTNVAWTGANIFGATTNPTVTINPA
                     TNTTYTVTATSNSCTQTVTKNITPVQCCTTANAYVMPAGTVLDNNFLNTPQSGLTIAK
                     GGTNPIFYDISPGFPNTSITINDEISIDAAEDENAVVRFFLTNSTTPTTINMAERARV
                     IVKPYNSVILKSTNVTACTNYLWRGFDIESYGSIQLDEQIPNSKPTISDAEYAIKLAH
                     DSKWKITSADFLNNYVSIYIPPTNLAEVNNNTVSTLYNSNFTGNGTSYHTNYAGQTTP
                     QFTYPYAGIILNDDALTLGNKNFNKLLFDKVNSGIMAYQTTMNVVNSQFTNIRLYNNV
                     VWNLTAAIYFDGINRHSTLTQEGFGYTNLPFTFESCTQGITCNRASMTIFKNNMNNMA
                     FGITASSSGFGDYINIHNCQIDCSARGITLLSTQNSEDIKISNNKLTTHNYNTWWANT
                     GGILVSETPVKMADTKLPRIELRNNVIKLDNNSEFGIKIFGIERGWLCSNDITCSTNL
                     PYAGIELNNVHNSSITLNTVAGNNATTSTTTGIRTILSPGNIFNGNTTTETKTGCYFD
                     LDCSGTDFKGNKFGKHYDGLLFGPNIIWNSQIHKGNQWSDNLADYGNYAAYFTPALFQ
                     ALSNQFVSNGIGTLCLPNTLFIPIVNGVKKYRPNTLSDGKDLFFPDPGAEYCSGTICE
                     YPARIGEGGDETDHTIATGEVNTTTYTAEVNYSADQELVSRVHDNDSLQTADTTMANF
                     YDQRMQTNIGRFDSISRLLGNSQNNAHKVSSIRYNDSLIQTYLTAINNFDMGNVVELK
                     KIITYRNEIAALQLENNNLKSILTNDLIANATLAKNINDAIVSAEMIEDIEQQVWAVY
                     IAMQIEQRKPNATELNIVLPIAQMCPAQGGRAVYIARTIFALVNDTIVYDDSAACANV
                     GYNFKVIPFKNVAADRLFKISPNPTKENLN"
BASE COUNT         1624 a          944 c          835 g         1346 t
ORIGIN      
        1 ctatcgaata ttttcaatcg caacgcgact ttcccatgat tgacaacacc ccagccagtg
       61 ttaccataag cgctctcaat gtttgtgctc cgttaatgcc agttttaaat aatgtaacag
      121 cctatcctgc tcatcaattt gctttgccca tgacttttca acaattaaat actgaagtta
      181 agaatataga aattggcgtt gagcagtaca acacgcattg cgaaaactta gtaacttttc
      241 ctacacctgc agattatgca attagtacag cacagtggat aaatgccgct aacgctttat
      301 ggccaattgt ccttccaaat acacataaca attataaatt tgctgtatca ggtaataagc
      361 cttacgagtt taatactgaa cgtagaacaa aatggaatga acaagtatat ccttatctta
      421 ataatacagc aatgcttgag ggtaaataca gcattatttt gcacccttat atgggcagtg
      481 gattatcagg tttaggtgta aacaatacaa ctaatattaa tgaaatgatg gtgcaaccat
      541 ttcaatatta tgataatttt ttggaaaagg aaatccatca gaatataaaa ccctacggca
      601 ttaatcatta ttttaatact accaaacccg acatttggat tacggaaggg aatatgtttt
      661 cgaaaactgg tgtggtgcat ggtagctggg ctgcagcact ttttacaggt accctgtttt
      721 taaaaatgat ggaagaaaat caaataaaaa ttcttgacca ccatgccatg gcaggcaact
      781 ttgtatgggc cgattatttt aattcgaatg atggttatat aacaggtgat tttgatgcac
      841 ctgatgttga tggtaataca acaaacggcc tgcaagtgcc cactactgtg aatttgaaag
      901 gtagcctcac tttacaagga catgtaatgc gtatgatagg ttcatgcttt cgcaatgctg
      961 cacaagcgca cttgctcaat tttactggtg taaatatggc agtaccaagt tatacaccaa
     1021 ttggcactac tgccatttat ccaatgcttt atggatggga atttgctgca agcagtgcca
     1081 ccgataatca aatactattg ttgaacttga cagaccaacc acaatctata gatgtttcca
     1141 gcttcaattt taatgctgtc aatacattag gatatcaatt ggcttttcct tttaattcct
     1201 ttttatcgga cgataataat ggctattact tgcatacata tagcactaat agttatactt
     1261 caaccaatag tactccaatt tatcagcaac cacttacctg gtcaaacgga tatataattt
     1321 tgcctgcata cagcattaca aaaatgcaga aatgtataaa caccccgcct gttttggttg
     1381 cttctaccac cgaaccttgc aatggtttac cattttcgat aaaggttgta aacaataaaa
     1441 attataccgg caactacacg tgggtatcaa gcaataatgc tagtactact accactacag
     1501 aacctgtact tgtaacaaat ggtataactg caaatactac gtatactgtt acagtaaata
     1561 tatgcattgg cggcactaca agtaatagta tactcattaa ggcccacacc aacttaacag
     1621 caaatttaac aacgaacact tcattaccgc tgtgcataac aggtaatgca gcacagttaa
     1681 cggcaacact tagtggcagc tccgctttca aatcatacgc catatatgcc ctcgatggtg
     1741 ccgacccggc atacgttgca cctgatgttg gctatggcaa taccaacaac agtatgtttc
     1801 taaccaatgt acatccaacc gcaaccaccc aatatcaggt aacggcatcg gatggggtgt
     1861 gcgctgttac ttcaccgcct attgcaataa ccgtggtgcc taaattagaa attaccccca
     1921 atgtagacca agtatgcaca ggaatatata ctgatttggc cgttgacaat acatttacaa
     1981 atgttgcctg gacaggggca aatatttttg gcgctactac taatccaact gtaaccataa
     2041 atcctgcaac aaatacaacg tatacggtta ccgctacaag taattcctgc acacaaacag
     2101 taaccaaaaa tataacacct gtacaatgtt gtacaactgc aaacgcttat gttatgccag
     2161 ccggtactgt gttggataat aattttttaa atacaccaca atcaggactt acaattgcta
     2221 aaggtggaac gaatcctatt ttctatgaca tttcccccgg gttcccaaat acatctataa
     2281 ccataaacga tgaaataagt atagatgctg cagaagatga aaatgctgtt gtaagatttt
     2341 ttctcaccaa ttctacaaca cccactacaa ttaatatggc cgagcgtgcg cgcgtaattg
     2401 taaaaccata taacagtgta atactaaaaa gtacaaacgt aacagcatgt accaattatt
     2461 tatggcgggg cttcgatatt gaatcgtacg gaagtataca attggatgaa caaattccaa
     2521 atagtaaacc cacaatatcc gatgccgagt atgccataaa acttgctcac gatagcaaat
     2581 ggaaaattac cagtgccgat tttttgaata attatgttag catttacata cctcctacca
     2641 acttggccga ggtgaataat aatacagtaa gcacattgta caatagcaac tttacaggca
     2701 atggtacaag ttaccataca aattatgcgg ggcaaactac acctcaattt acttaccctt
     2761 atgcaggcat aatacttaat gacgatgctt taacattagg aaataaaaat ttcaacaaac
     2821 ttttgtttga taaagttaac agtggtataa tggcttatca aacaacaatg aatgtggtaa
     2881 actcccaatt cacaaacatt cgattgtata acaatgttgt gtggaattta actgccgcca
     2941 tttactttga cggtattaac agacattcta ccttaacgca agaaggattt gggtatacga
     3001 acttgccttt cacctttgaa agctgtacgc aaggcattac gtgcaatagg gcaagtatga
     3061 ctatttttaa aaacaatatg aacaacatgg cttttggcat tactgcttct tcatctggtt
     3121 ttggcgatta tataaatata cacaattgcc aaattgattg tagtgcgcgt ggtatcactt
     3181 tactatcaac acaaaactcc gaggatataa aaattagcaa taataaatta acaacacata
     3241 actacaacac ttggtgggca aacaccggtg gtattctggt aagcgaaacg cctgttaaaa
     3301 tggccgatac aaagctgcct cgtatcgaat taagaaataa cgtaataaag ctcgataaca
     3361 atagtgagtt tggtattaaa atatttggta tagaacgagg ttggttatgc agcaatgata
     3421 ttacatgcag caccaactta ccctatgcag gtatagagtt aaataatgtg cacaactcat
     3481 ctatcacact taatacggta gcgggcaaca atgctaccac atctacaacc accggtatac
     3541 gcactatttt aagccccggc aatattttta acggcaatac caccaccgaa actaaaacag
     3601 gatgttactt cgacctcgac tgtagtggca ccgattttaa aggcaataaa tttggaaagc
     3661 attacgatgg tttattattt ggacctaata ttatttggaa ttcgcaaata cataaaggta
     3721 accaatggag cgacaattta gcagattacg gaaactacgc agcttatttt acaccagcac
     3781 tttttcaagc tttatctaat caatttgttt caaatggaat aggaactttg tgcttaccaa
     3841 atactctttt tatacctata gtcaacggtg taaaaaaata tagaccgaat actttatcag
     3901 atggaaagga tttatttttc cctgaccctg gtgccgaata ttgctccgga acaatatgtg
     3961 aatatcctgc aaggatagga gaaggtggcg atgaaactga tcacacaatt gcaacagggg
     4021 aagtaaatac cactacctat acagccgaag taaattatag tgccgaccag gaactggtaa
     4081 gtagagtgca tgataacgat agcttacaaa ctgcagacac cactatggcc aacttttatg
     4141 accaacggat gcaaactaat attggcaggt tcgattctat tagtagatta ttaggcaatt
     4201 cgcaaaataa tgctcacaaa gtttcatcca taagatataa cgattcgttg atacaaactt
     4261 acctcacagc aataaataat tttgacatgg gaaatgttgt tgaacttaaa aaaataatta
     4321 cttatagaaa cgaaattgca gcattgcaat tagaaaacaa caaccttaaa agtattctga
     4381 caaatgattt gattgccaat gcaacgcttg ccaagaacat aaacgatgcc attgtaagtg
     4441 ccgaaatgat tgaagatatt gaacagcagg tatgggcagt ttatattgcc atgcaaatag
     4501 aacagcgaaa gccaaatgca accgaattaa atattgtgct gccaatagca caaatgtgcc
     4561 ctgcacaggg cggcagggct gtttacatag cgcgcaccat atttgcacta gttaacgata
     4621 ctatagttta tgatgatagt gcagcttgtg caaatgtggg gtataatttt aaagttatac
     4681 catttaaaaa tgtggcagcc gataggttat ttaaaattag ccccaatcca actaaagaaa
     4741 acttaaatt
//
LOCUS       DAORQA010000132         4738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4487_length_4738_cov_13.892377, whole genome shotgun sequence.
ACCESSION   DAORQA010000132 DAORQA010000000
VERSION     DAORQA010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4487_length_4738_cov_13.892377"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..1419
                     /locus_tag="PK736_06880"
     CDS             289..1419
                     /locus_tag="PK736_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRC33151.1"
                     /translation="MNFIQVCQADELPDNSQKIVNIGKRKIALFHFNGKLSAMANACL
                     HKAGPLGLGVVGAKYDGMYVTCPWHGWEYNIVTGSAPPGYPDQQALYEIKSEGGYILV
                     SEEPIVKAHRVHQNNPLLEDLIKLKYQTSANSLNILGISTTNLSPNLPRFSTSEDALE
                     KSLAYASEKYGAATKMVKLRELEFRNCEGYYSQHSHACTWPCSITEMNPEDGMTEVYE
                     SMILWADIVIVSTPIRWGNASALYYKMAERLNCVQNQISINSKILVKNKIAAFIITGG
                     QDNIQQVAGQMMCFFTDLGFTFPPFSFLGWSRGWTAEDMDKNVMQFKKSEYIKRTSIE
                     MVDNCVEVLKQIKNINSSNIHAPKPKRSDSLSIDIENKDMNI"
     gene            1965..2903
                     /locus_tag="PK736_06885"
     CDS             1965..2903
                     /locus_tag="PK736_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33152.1"
                     /translation="MASPIESRIKNIQKSLKVTQTGVFDMATCKAFELLQSVNVTSAT
                     LTDHLKAIQKFLGFTGKDVDGIYGVATISRIEQIFDATVPTLPANTSMVLSNKGIENI
                     LQWEIGGKAAYISRFTNPIWPKESSGVTIGIGYDLGYCTAAKFADDFKKLPQADINKL
                     STVIGLKGAAAQRALTPNIKSASVPWEIAYEVFYSSSIPEHAKLTKRIYPEVSQLPPD
                     AQSAIVSLIYNRGTKLSGDRRVEMYNLIQLIKDKKLKAIAAEFRKMKRLWEPKKDKDG
                     NITDKGSRGLIIRREAEAVLIENATWVMNPSQYIFA"
     gene            complement(3186..4235)
                     /gene="rsmH"
                     /locus_tag="PK736_06890"
     CDS             complement(3186..4235)
                     /gene="rsmH"
                     /locus_tag="PK736_06890"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRC33153.1"
                     /translation="MNKDEPKPPRRVRYKGTHPKSFKEKYKELQPDLYADDVEKIMQK
                     GYTPAGMHRSICVNEIMQFLNVQPGQTGLDATLGYGGHTLEILKRLLPGGILYATDVD
                     PYELPRTRERLDALGYGEDVLRIKKMNFADVDQIAAEAGPLNFVLADLGVSSMQIDNP
                     ERGFSLKLDGPLDLRLNPKSGKPVSEILKIISQAEIEEILFENADEPYADVIAEAITL
                     HIKKENQIGTTTQLKEVIKDALQFIHPNERAEKVKKSCRLCFQALRIYVNDEFGVLEK
                     FLEKLPYILAPHARVAILTFHSGEDRRVKKSFQNYFRAGIYSDIAPEPMRPSLEEISS
                     NPRARSAKLRWAVKS"
     gene            complement(4379..>4738)
                     /locus_tag="PK736_06895"
     CDS             complement(4379..>4738)
                     /locus_tag="PK736_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33154.1"
                     /translation="IDGDKKNDSIYFIGNGGVHTFYYLRLILSSSGVPKDYTFLELDM
                     PYISEKKLLDEFGKNPAVQFVVYDFDNDGILDLYLNFSASSIKVPDSWRMQGVTTKCV
                     VMHFTKVKIQVSDYKFR"
BASE COUNT         1648 a          827 c          865 g         1398 t
ORIGIN      
        1 gaaacaaatt atatctaaat aaaggttatg tggacaccgg gcgatatttg ctgacaaaat
       61 cagttaatat tataaaagtg gaaaaataag accgaactac taacatcggt ttggcaatat
      121 agcggctgaa gtgcttctat gaaaattttg tgtgaggttc aacagtagga attcaattga
      181 atcccgaaag ctttcgggat gctaaaaatc caccacatcg ccaagccgaa aaaagttaag
      241 cataattaaa gacagtaagt tttttaaatt gattcaaaaa atattaaaat gaatttcatt
      301 caggtttgcc aggcagatga attgccagat aactctcaaa aaatagtcaa cataggaaaa
      361 agaaaaatcg ctctttttca ttttaatgga aaattatcgg ctatggccaa tgcatgtttg
      421 cataaagccg gaccattagg tttaggagtg gtaggtgcta aatatgatgg tatgtatgta
      481 acctgtccat ggcatggttg ggaatacaat atagtaactg gcagtgcgcc tcccggatat
      541 ccggaccaac aagcacttta tgaaataaaa tcggaaggcg gatatatatt ggttagcgaa
      601 gaaccaatag ttaaagcaca tagagtgcat cagaataatc ctttactgga ggatttaata
      661 aaactcaaat accagacttc agcaaatagt ttaaatattt taggcatcag cacaaccaat
      721 ttatcgccca acttaccaag gttttctact tcggaagatg ctttagaaaa atcactggct
      781 tatgcttccg aaaaatacgg agcggcaact aaaatggtca aattaagaga attggaattt
      841 cgtaattgtg aaggatatta ttcacaacat agccatgcct gtacctggcc ttgcagcata
      901 accgaaatga atccggaaga tggtatgacc gaagtttatg aaagcatgat tttatgggct
      961 gatattgtaa tcgtatcaac tcctatccgc tggggcaatg cttctgcatt atattacaaa
     1021 atggcggagc gattaaattg tgtgcaaaac caaatttcta tcaatagtaa aatattagta
     1081 aaaaataaaa ttgctgcatt tatcattact ggcggacagg ataatataca acaagtagcc
     1141 ggtcagatga tgtgcttctt taccgacctc ggatttactt ttcctccatt tagttttttg
     1201 ggttggagtc gcggatggac tgcagaagac atggacaaaa atgtaatgca atttaaaaag
     1261 agtgaataca ttaagcgaac ctcaatagaa atggttgaca attgcgtgga agtattgaag
     1321 caaattaaaa atataaacag ctcaaatatt catgctccaa agcccaaacg aagtgattcg
     1381 ttaagtatag acatagaaaa caaggacatg aatatttaag atttaaaaaa ttacgtttac
     1441 agcgggtttt gctttagcgg gcaggcagtg tgaatagtaa catttgagca aggtggaagt
     1501 tcagttctcc gaattaagtt tagtgctaaa actccgccac atcgcaaagc ataaaacacc
     1561 gggatgtaaa aaaatctgtt ttaaaataat tagtcggtca caaaccgggc aatatctatg
     1621 actgtatacg ttttatgtca tcattagcta tttaatttat cagaacaaca attacacttt
     1681 caaatttcaa ctgcactaat tcgagtttat cgacaaatat atcgataaag caaacatgat
     1741 tgtgtgcaga aacacgacaa tcgaaaagta taatctacct gatgtctatt ggctttagca
     1801 aagctatgca tgcaaaatat tttttttcaa aaatactgaa cagcagttgt tatgtttctt
     1861 atttttacaa aatgctatat cggccagctg ttgagaaaat ggttttcaca gtctgcaaga
     1921 tgataaacag agtaggcaaa aaaataaata aataaattaa aacaatggca tcaccaatcg
     1981 aaagcagaat taaaaatatt caaaaaagtt taaaagtaac acaaacaggt gtgtttgata
     2041 tggccacttg taaagcgttt gaactgttac aatctgtaaa cgtaacaagt gcaacattaa
     2101 ccgatcactt aaaagcaata caaaaatttc ttggatttac gggcaaagat gtagatggaa
     2161 tttatggtgt tgcaaccatt tcgcgtatcg aacaaatttt tgatgccacc gtccctaccc
     2221 tgccggctaa tacatcgatg gtgctttcta acaaaggcat cgaaaatatt ttgcaatggg
     2281 aaattggcgg aaaggcagct tatatatcaa gatttacaaa ccctatctgg cccaaagaat
     2341 cgagtggtgt aacaattgga attggttatg atttaggata ttgcacggcc gctaaatttg
     2401 ccgatgattt taaaaaatta ccacaagcag atataaataa attaagcact gtgattggat
     2461 taaaaggtgc ggcagctcag agagcattaa ctcccaatat taaatcggca agtgttccat
     2521 gggaaattgc atatgaagta ttttattcca gttcaattcc tgaacatgct aaacttacca
     2581 aaagaatcta tcccgaagta tcgcaactac cacccgatgc acagtcagct attgtatctt
     2641 tgatctataa cagaggcaca aaactttccg gtgacagaag ggtagaaatg tacaatctga
     2701 ttcagcttat caaggataaa aaattaaaag caatagcagc agagtttaga aaaatgaaac
     2761 gtttgtggga acctaaaaaa gataaagatg gcaacattac cgataaggga tcacgcggat
     2821 tgataatacg cagagaagcc gaagcagtat tgattgaaaa tgctacatgg gtaatgaatc
     2881 cttcacagta tatttttgct taagtacttt ttattcaaga aatttattcg attgatagtt
     2941 gttgttcaac cccaattaaa tagattaaaa acatttcgct taagctaatt tcaaagagta
     3001 tgacgtgctt tacgatggtg aaactttatc atttagggtt ttgctaccaa acataaaata
     3061 gcggagttgg taagaaaaat aaattattga aattaataat cctgttgtcg aataacaatt
     3121 tatatatgtt caaaattttc aaggcattta tttggaataa atgtatagaa aaaaaataaa
     3181 tccattcaac tcttcacagc ccacctcaac tttgcagaac gagcacgggg attgctgctg
     3241 atttcttcta acgaaggacg cataggttcg ggtgctatat cgctgtaaat gccggcacga
     3301 aaataatttt gaaaagattt ttttacgcgc ctgtcttcgc ccgaatgaaa tgtaagaata
     3361 gctacacgtg catggggtgc taatatgtaa ggaagttttt cgagaaattt ttcgagcacg
     3421 ccaaactcat cgttcacata tatacgcaat gcctgaaagc aaagccggca agattttttt
     3481 actttttcgg cacgctcatt aggatgtata aattggagag catctttaat cacttctttt
     3541 aattgcgttg tagtacctat ttgattttct tttttaatat gtaatgtaat tgcttcggct
     3601 ataacatctg catagggttc atcggcattt tcaaaaagta tttcttctat ttcagcttgc
     3661 gaaataattt tcaaaatttc cgaaacaggt tttccacttt ttggattgag acgtaaatcg
     3721 agtggcccat ccaacttcaa cgaaaatccg cgttcgggat tatcaatttg catggacgat
     3781 actccaaggt cggcaagcac aaaatttaac ggccctgcct cagcagcaat ttgatcaaca
     3841 tcagcgaagt tcattttttt tattctcaaa acatcttcgc cataacccaa agcatctaag
     3901 cgttcgcgtg tacggggtaa ctcataagga tcaacatcgg tagcatacaa aatgccaccg
     3961 ggcaataaac gtttcaaaat ttccagagtg tgtccaccat aaccgagtgt tgcatcaagt
     4021 cctgtttggc cgggttgtac atttagaaat tgcataattt cattcacaca aatagaacga
     4081 tgcataccgg caggcgtgta acccttctgc ataatctttt ctacatcatc agcatataaa
     4141 tcgggctgta gttctttata tttttcttta aaagattttg ggtgtgtgcc cttgtatcgt
     4201 actctgcgtg gtggtttcgg ttcatcttta ttcatggtgt aaaaataaat ataatcacat
     4261 catatcacca tcgaatttta aaaattaaaa aactctttgc aaataaaatc tgccactaca
     4321 aaattctttt ttagtattca ctgcaatttt caaaagtgat tagcgaaaaa ataatttgct
     4381 accgaaactt ataatcactc acttgaattt ttacttttgt aaaatgcata accacacact
     4441 ttgttgtaac gccttgcatg cgccagctat caggaacttt tattgatgaa gcagaaaaat
     4501 taagatataa atccaaaata ccatcgttat cgaaatcgta aaccacaaat tgaactgcgg
     4561 ggtttttacc aaactcatcc aacaattttt tttccgatat gtaaggcata tcgagttcta
     4621 aaaaagtgta atcttttgga acgccactgg aagataaaat caaacgcaaa tagtaaaaag
     4681 tatgaacacc gccattgcca ataaaataaa tggaatcgtt ttttttatcg ccatctat
//
LOCUS       DAORQA010000133         4630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4618_length_4630_cov_11.077158, whole genome shotgun sequence.
ACCESSION   DAORQA010000133 DAORQA010000000
VERSION     DAORQA010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4618_length_4630_cov_11.077158"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PK736_06900"
     CDS             complement(<1..226)
                     /locus_tag="PK736_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33155.1"
                     /translation="MVGTEPEEGVKDKELANPLPDVNDTSKPVGGVTNKFAVRPVPET
                     VNDCSAETLPWHDTNGINTPVVVILAPPIAE"
     gene            243..>4630
                     /locus_tag="PK736_06905"
     CDS             243..>4630
                     /locus_tag="PK736_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRC33156.1"
                     /translation="MTAAASGTPSGNIACTSTGATTQDVAVSGTVYNPTLTVNAQTNV
                     SCFGGTNGAASVNAAAGGLAPYSYNWTPGNPAGDGTVSVTGLTAGSWTCTVTDNNGCT
                     SSISFIITDPTVIVANCSGTNVSCNGGNNGSASVSASGGTGTLTYLWNNSNTNSSISG
                     LTAGTYTVTVTDANGCTQTCAYTVTQPTTIVANCSGTNVSCDGGSNGSASVAASGGTG
                     TLTYFWSNTNTNSSISGLSSGTYSVTVTDANGCTQTCAYTVTQPTTIVANCSGTNVSC
                     NGGSNGSASVAASGGTGTLSYLWSNLNTNSSISGLTAGTYTVTVTDANGCTLSCSYIV
                     TQGPAISLSNVPTNVSSCFTNNGAINLTVSGGVPCGANSKGLLISKIFANPNGTDSPF
                     EFVQLVATKNIDFSVNPYTVIVSNNGIATVNGWKAGAAITYAFEINTGTANGGDIYYV
                     GGSSMVPVANRLRSINTSTTGGDGGVGNLSASGVFGNGGTSDGVAVFAGTIASINSTT
                     VPVDATFFGATRTNAIVGGGAGGYELPVNDFYPTGGKFNATSFTFGTDPGNDIIFNIN
                     GTYNATTNVFTTARAFTSNNISTFNAATTGLTLTNFAAYTYLWSNGATTEDVTGLSPG
                     TYTVTVTDCNGCTATMSQTIVAPAAPAVTCSSSNNTSCTGANGTASATTSAASPTYVW
                     SNSATAASITGLAAATYTVIVTDGVTGCTASCTSVVANSILNPTATCSSTDNSNCTPA
                     NGSVSVTTSASSPTYSWSNSATAASVSALGAATYTVMVTETTTGCTVTCSATVINTSV
                     SITASCTAVDNTSCSTPNGSASVTTSATSPTYAWSGGGTSATITGLAGGTYTVLVTDG
                     VTGCTATCAAFVGSSTINPTATCSSTDNTNCTSANGSASVTTNATSPSYSWSNGNSNS
                     SISGLAAGTYTVIVTETGTGCTATCEAVVANATVNPTLTCSSTNNTSCLSPNGSASVS
                     DNATSPTYSWSNNASTNSISGLGAGTYTVTVTDGVTGCSASCTSVVNNVQSTPTATCS
                     GSAITCNGTNNGTANILANGGTPTYTYSWSNSNTNSSISGLAAGTYTVTVTDANGCTA
                     QCSYTVTTPAAVGAVCSSTNITCFGLNDGTNSVSASGGTPPYTYAWNGGSTNASNTGL
                     SAGTYTVTVTDANSCFATCSVTISEPPLVSITVSNNSPFCAGGDLAFSVTPSVGGLLY
                     SWSGPNAFSSSLQNPVLLGAMSNASGTYTVTATDLSGCSASATSMVSVYFSPALPIVT
                     TPVNYCLNSVSTPLTAIGDSLKWYTDSTISTPLSTAPTPLTTVAGQTCYFVSASKYTP
                     SPVSSISAGDIAIIGRQNGATDAVQFVTLVDMPAGTVIYFTDNGWTGTGFRGSSATDG
                     NGFEGLIKFTSNAIIPAGNVITSISTSPSYTWVTSGGTIPGTSTPWGGVDLAAAGDQI
                     YAFRSDSVNNPLFHASAITHLFVWDDT"
BASE COUNT         1268 a         1003 c          997 g         1362 t
ORIGIN      
        1 attctgctat tggcggtgca agtattacaa caacaggagt attaattcca tttgtatcat
       61 gccagggaag tgtttctgca gagcaatcat ttacagtttc tggtacaggc cttacggcaa
      121 atttattagt tacaccacca actggcttcg aagtatcgtt aacatccgga agtggatttg
      181 caagttcttt atcttttact ccttcttccg gttcagtgcc caccaccaca atttttgtcc
      241 gtttgactgc tgctgcatca ggaactcctt ccggaaatat tgcatgcaca tcaacaggag
      301 ctactactca agatgttgct gtaagtggaa cagtgtataa tcctacttta acagttaacg
      361 ctcaaaccaa tgtttcctgc tttggcggaa caaacggagc agcaagtgta aacgctgcag
      421 ctggcggatt agcaccatac tcgtataact ggacacccgg aaaccccgca ggtgatggaa
      481 cagtttcggt tacaggatta acagccggta gctggacttg taccgttacc gataacaatg
      541 gatgtacctc aagcatcagc tttattatta ccgacccaac agttattgtt gccaattgtt
      601 caggaacgaa tgtgtcttgt aacggtggta acaacggttc tgcatcagta tcagcaagtg
      661 gtggtacagg cactttaact tatttgtgga ataattccaa taccaattct tcaataagtg
      721 gattaacggc tggtacgtat actgttacgg ttaccgatgc aaatggttgt acgcaaactt
      781 gtgcttacac tgttacgcaa cccacaacaa ttgttgccaa ttgttcaggt acaaatgttt
      841 cctgcgatgg aggtagcaac ggttcagcat ctgtagctgc aagtggaggc acaggtactc
      901 ttacttattt ttggagtaat acaaatacta attcttctat aagtggacta tcatcaggaa
      961 cctactcggt tactgttacc gatgctaatg gttgtactca aacttgtgct tacacagtta
     1021 cacagcccac aacaattgtt gccaattgtt caggaacgaa tgtttcctgc aatggtggca
     1081 gcaatggttc agcatctgta gctgcaagtg gtggaacggg aacactatct tatttatgga
     1141 gcaatttaaa tactaattct tcaataagcg gactaacagc cgggacgtat actgttacgg
     1201 ttaccgatgc aaatggttgt actttgtctt gttcatatat cgtaacccaa ggccctgcaa
     1261 tttcgttgtc aaatgtgcca accaatgtat catcatgctt tactaacaat ggggcaataa
     1321 atttgaccgt tagcggaggc gtaccttgtg gtgcaaattc taaagggcta ttgatttcta
     1381 aaatatttgc aaaccccaat ggtacagact cccctttcga atttgttcaa ttagttgcta
     1441 cgaaaaatat cgacttctca gtaaatccgt atactgtgat tgtttctaac aacggcattg
     1501 caactgtaaa tgggtggaag gcaggtgctg ccattaccta tgcttttgaa attaatacgg
     1561 gtaccgccaa tggtggcgat atttattatg taggcggttc atctatggta cctgttgcaa
     1621 atagactgcg ttcaataaat acaagtacaa caggtggtga cggtggcgtt ggcaatttga
     1681 gtgcgtctgg tgtttttgga aatggtggaa catctgatgg tgtggccgtt tttgcaggta
     1741 ctattgcttc aataaattct acaacagtgc ctgtggatgc aacttttttt ggtgcaacac
     1801 gaaccaatgc aattgttggc ggaggagcag gcggatatga attgcctgta aatgattttt
     1861 atcccacagg tggaaagttc aatgctacta gttttacatt tggcaccgat cctggtaacg
     1921 atattatttt taatattaat ggtacttata atgcaacaac aaatgtattt acaactgcta
     1981 gagcatttac atccaacaac atttcaacat ttaatgctgc aaccaccgga ttgactttaa
     2041 caaactttgc agcctatact tacttatgga gcaatggagc aaccacagaa gatgttacgg
     2101 gtttatcgcc aggaacgtat actgttacag ttaccgactg taatggttgc acagctacca
     2161 tgtcgcaaac tattgttgca ccggcagccc ccgcagtaac ttgttcatca tcaaataata
     2221 caagttgcac gggagctaat ggtacagcaa gtgcaacaac cagtgcggca tcaccaacat
     2281 atgtttggag taattctgct acagctgctt ctattacagg attggctgct gcaacgtata
     2341 ctgtaattgt tactgatggt gtcacaggtt gcacagcatc ctgcacatcg gtggttgcaa
     2401 atagtatttt aaacccaacg gcaacatgtt catccacaga taactcgaat tgtacaccgg
     2461 caaatggaag tgttagtgtt acaacaagcg cttcatcacc aacttattca tggagcaatt
     2521 cggctactgc tgcaagtgta tcggcattag gtgcagctac atatactgtc atggttactg
     2581 aaaccacaac cggctgtaca gtaacttgtt cggctacagt gattaacaca agtgtaagca
     2641 tcacagcatc atgcacggca gtagataata caagttgcag tactccaaat ggtagtgctt
     2701 cggttactac ttctgctaca tcccctactt atgcctggag cggtggtggt acatcggcta
     2761 caattaccgg cttggctggc ggtacttata cagtgttggt gaccgatggt gtaactggtt
     2821 gtacagcaac ttgtgcagct tttgtgggca gctcaacaat aaacccaaca gctacatgtt
     2881 cttctacaga caatacaaat tgcacaagtg caaatggtag tgcaagtgta actacaaatg
     2941 caacctcacc ttcttactca tggagtaatg gcaattccaa ttcaagtatt agtggactgg
     3001 cggctggcac gtatactgtt atagttactg aaaccggaac gggttgcact gcaacctgtg
     3061 aagcagttgt tgcaaatgca acagtgaatc caactttaac atgctcgtca acaaacaata
     3121 caagttgttt atcgcccaat ggctcagcat cggtttcgga caatgccact agcccaacat
     3181 attcatggag taataatgca agcacaaatt ctatttcggg gcttggtgcc ggaacctata
     3241 cagtgaccgt taccgatggt gttacaggat gttcagcatc gtgcacatct gttgttaaca
     3301 atgtgcagtc aacacctact gctacttgct caggttcagc aattacttgc aatggcacca
     3361 acaatggtac tgcaaacatt ttagcaaatg gcggtactcc aacctatact tattcatgga
     3421 gtaattcgaa tacaaattct tcgataagtg gtttagcggc cggtacttat actgttactg
     3481 ttacagatgc caacggatgt accgcgcaat gcagctatac tgttacaacg cctgctgctg
     3541 taggtgcagt atgctcttct accaatatca cttgttttgg tttaaatgat ggcaccaatt
     3601 cggtgagtgc atcgggtgga acacctcctt acacttacgc ctggaatggt ggaagtacca
     3661 atgcaagtaa cactggctta tctgcaggta cgtatacagt tacagtaact gatgccaaca
     3721 gttgtttcgc aacttgttcg gttactatat cagaaccacc attagtttca ataacagttt
     3781 cgaacaacag ccccttctgt gccggaggcg atttagcttt cagtgttaca ccttcggttg
     3841 gtggtctttt atattcatgg agtgggccaa atgcattttc ttcttccttg caaaacccgg
     3901 tgttgcttgg tgctatgtca aatgcatcag gtacgtatac tgttactgct acagacttaa
     3961 gtggatgttc ggccagcgca acttctatgg tatcagttta tttctcgccc gcattaccca
     4021 ttgttaccac accggtaaat tattgtttaa atagcgtatc cacaccactc actgcaattg
     4081 gcgatagttt gaaatggtac acagattcaa ctatttcaac tccgttgtca actgcaccaa
     4141 cacctttaac gacagttgca ggccagacat gctattttgt ttcggcatct aagtatacac
     4201 caagccctgt gtcaagtatt tcagctggtg atattgccat tattggtaga cagaatggtg
     4261 caacagatgc agtgcagttt gtaactttgg ttgatatgcc ggccggcaca gttatttatt
     4321 ttacagataa tggatggaca ggcacaggtt ttcgtggctc gtctgccaca gacggaaatg
     4381 gctttgaagg attgattaaa ttcacatcca acgcaataat ccctgccggt aatgtgatta
     4441 cttctatttc tacatcgcca agttatacat gggtaacatc gggaggaaca ataccgggta
     4501 cttcaactcc ctggggaggt gttgacctgg ccgctgccgg tgatcagatt tatgctttcc
     4561 gttccgattc tgtgaataac ccattgttcc atgcatcagc tattactcat ctttttgttt
     4621 gggatgatac
//
LOCUS       DAORQA010000134         4620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4626_length_4620_cov_24.707777, whole genome shotgun sequence.
ACCESSION   DAORQA010000134 DAORQA010000000
VERSION     DAORQA010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4626_length_4620_cov_24.707777"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..913
                     /locus_tag="PK736_06910"
     CDS             83..913
                     /locus_tag="PK736_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33157.1"
                     /translation="MVFNNLKTAMLLLTASALFAVSCNVNDNNEKEFSAADGITHNDA
                     KNEKTYSEYLEKTADLNFKTNNTKQTHRYINNMATLLNGVVVESQMRPKIERSNTTQL
                     AHDSIQIVKIMSLNALMTVKIPTENCDAFIDSVAKKMTEPELMQFKVNDLTRNFIAAK
                     VYDKQNATNSSASTNANIYEQNRKVEYSTIHLSFYEPAGITYSRELDVEKLAHAETAF
                     AVLASDAFEKSVKSIRIFLLFVIRYWSVIAIISLVVFLFRKLIVRNKIMLPKYSSSQV
                     "
     gene            1220..2767
                     /locus_tag="PK736_06915"
     CDS             1220..2767
                     /locus_tag="PK736_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRC33158.1"
                     /translation="MSDTQKFIASMAEGYAFKGDTITIGAAMLDGQAVTEATIKAPLK
                     TFNRHGLIAGATGTGKTKTMQGIAEALSAKGVPCLMMDVKGDLSGLAEPGSGNLKIDE
                     RTAKIGTTWKPTAFPVEMFTISEENGVRMRATVTEFGPVLFSKILELNDTQEGVVSLI
                     FKYSDDKQMPLLDLKDFKKMLQYLTNEGKDEIKQEYGAISTSTASTILRKIIEIEQQG
                     AEKFFGEKSFEVEDLTRMDDRGYGYLSIVRLTDMQSKPKLFSTFMLSLMAEVYANFEE
                     VGDADAPRLVIFIDEAHLIFNTASKALLDQLETMIKLIRSKGVGIFFITQIPTDIPAN
                     ILSQLGMKVQHSLRAFTAVDRKAIKQTAENYPITEYYKTDELLTSLGIGEALFTALSE
                     KGNPTPLAHVLLCAPQSRMDILTPAELQTLVTNSKISKKYNEAIERDSAFEMLIKKLE
                     THVEEQKEKEEVKKEKEEPSTFEKVASSSAAKQVMRTAASIITRSLLGAIGIKSTTSR
                     RKKTGWF"
     gene            2817..3497
                     /locus_tag="PK736_06920"
     CDS             2817..3497
                     /locus_tag="PK736_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC33159.1"
                     /translation="MTQIVLLHGAMGSSSQMQGLGKLLEPHFEVFAFDFCGHGARASE
                     YISFSVQEFAADLDEFLMQKKIINPFIFGYSMGGYVALYHSLHFKNGAKKIFTLGTKF
                     NWSPAIAEKEVALLDTHVMQTKIPKFVEVLKNRHGEKMWDDVVKKTQDMMHLLGNNYQ
                     LDDEDLANIKCRVVLMLADDDNMVTEEETEQAHDSIAHSEYVIIQNSKHPIEKVDLEE
                     LVKVILKF"
     gene            3669..>4620
                     /locus_tag="PK736_06925"
     CDS             3669..>4620
                     /locus_tag="PK736_06925"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRC33160.1"
                     /translation="MSKNLLDFEKPIIELHEQLEKAKQIAEKGKVDTGQLIRELEQKI
                     VSTRKEIYEHLTPWQRVQVSRHPERPYTLSYIEFLCGKTFTELFGDRTVGDDKAMVGG
                     FGMLDDRTVMFIGQQKGNTTKQRQFRNFGMPNPEGYRKALRLMKLAEKFNKPIVTFID
                     TPGAYPGIEAEERGQGEAIARNLYEMSILKVPVICIIIGEGASGGALGIGIGDRVLML
                     ENTWYSVISPESCSSILWRNWDNKERAADALKLTANDMFANHLIDGIIPEPVGGAHSN
                     KEEIFATVKTYIINHLDELLKLDPETRIKQRIDKFCKMGVV"
BASE COUNT         1521 a          815 c         1000 g         1284 t
ORIGIN      
        1 ttacttctat atcggtttgc gtatgaatag taaagccggc tgatatattt tctttaatca
       61 aaaaaatcta aaactattta ttatggtatt taataattta aaaacagcaa tgttgctgct
      121 gacagccagc gcgttattcg cagtgtcgtg taatgtgaac gataataatg aaaaggaatt
      181 ttcggcagca gatggtatta cgcacaacga tgcaaaaaac gaaaagacat acagcgagta
      241 tttggaaaaa actgccgacc tcaattttaa aacaaacaac accaaacaaa cgcacaggta
      301 tataaataac atggcaactt tgttgaatgg cgttgttgta gaatcgcaaa tgcgaccaaa
      361 gattgagcgc agcaacacca ctcaactggc acacgattca atacagattg taaaaatcat
      421 gagtttgaat gcgttaatga cagtaaaaat tccaaccgaa aattgcgatg catttataga
      481 ttcggtagca aaaaaaatga cagagcccga gcttatgcaa tttaaagtaa atgacctgac
      541 aagaaatttt attgcagcaa aagtttatga taaacagaat gctacaaata gttcggcatc
      601 gaccaatgca aatatttatg agcaaaacag aaaggtggag tatagcacca ttcacttaag
      661 tttttacgaa cctgcgggca taacttattc gcgcgaactt gatgtagaaa aattagccca
      721 tgccgaaaca gcatttgcag tgcttgcttc cgatgcattc gaaaaaagtg taaaaagtat
      781 acgcatattt ttactgtttg taatccgata ctggagtgtg attgcaatta tttctttagt
      841 ggtttttctt ttcagaaaat tgattgttcg caataaaata atgttgccaa aatattcttc
      901 gtcacaagta tagacaaaac tgtttaaccg aagcaaattc gcatgccaac actttaatgt
      961 ttgatagaag gaatgcgtga gggatacaag cggaaatcct ttttgtgagg aacgagcaaa
     1021 aagattgaag cggtagcccg accttgctac tgcaagggca cgcccaacta tttatttttg
     1081 aattgtttta tagttttggt ttgcaaatta tgaagcctct ctttcaacaa atttttgttg
     1141 ttcaatgttg aaaaataaat ctttttttag ccgttgtaaa actacttttg caggtcaaga
     1201 taaaatcagg atttataata tgagtgatac acaaaaattt attgcaagca tggcagaggg
     1261 ttatgctttc aaaggcgata ccataacgat tggtgccgca atgcttgatg ggcaggcggt
     1321 tactgaggca accataaaag cacctttaaa aacttttaac cgccacggtc ttattgccgg
     1381 tgctaccggt accggaaaaa caaaaacaat gcagggcata gccgaagcac tttcggccaa
     1441 gggtgtgcct tgccttatga tggatgtgaa aggcgatctt agcggacttg ccgaacccgg
     1501 tagtggcaac ctgaaaattg atgaacgtac tgcgaaaata ggaacaactt ggaaacccac
     1561 tgcttttccg gtggagatgt ttaccatttc ggaagagaac ggtgtacgca tgcgtgctac
     1621 ggtgactgaa tttggacctg tgttgttttc caaaattttg gagctgaacg atacacagga
     1681 aggtgttgtt tctctgatat ttaaatatag cgatgataag caaatgcctt tgctcgactt
     1741 aaaagatttt aagaaaatgc tgcagtacct taccaatgaa ggtaaggatg aaatcaaaca
     1801 agagtatggt gccatttcta cctcaacagc atcgacaatt ttgcgtaaaa ttattgaaat
     1861 tgaacaacag ggagcagaaa agttttttgg tgaaaaatcg tttgaggtgg aagaccttac
     1921 acgcatggat gaccgtggtt acggatatct tagcattgtg cgccttaccg atatgcaaag
     1981 caagccaaaa ttattttcca ctttcatgtt aagtttaatg gcagaggttt atgccaattt
     2041 tgaagaagta ggcgatgccg atgcaccccg tttagtgatt tttattgacg aagcgcattt
     2101 gatttttaac actgcttcga aggcattgct cgatcagttg gaaacaatga taaagttgat
     2161 tcgctcgaag ggtgtgggta tattttttat tactcaaatt cctaccgata ttcctgcaaa
     2221 tattttaagt caacttggta tgaaagtgca acacagcttg cgtgcattca ctgctgttga
     2281 tcgtaaggca ataaagcaaa cggccgaaaa ttaccccatt accgagtatt ataaaactga
     2341 tgaattgcta acatcgctcg gaattggtga agcattattt acagcgctta gtgagaaggg
     2401 aaatcctaca ccacttgcac atgtgctgtt atgtgcaccg cagtcgcgaa tggatatact
     2461 cacccctgcc gaattgcaaa cattggtgac caattcaaaa atcagcaaaa agtataatga
     2521 agcaatcgaa agggacagtg ctttcgaaat gttgattaaa aaacttgaga cacacgttga
     2581 agagcaaaaa gaaaaagagg aggtgaaaaa ggagaaagaa gagccaagta cttttgaaaa
     2641 agtagcaagc agctcggcag ctaagcaagt aatgcgtaca gcagcaagta ttattacccg
     2701 aagtttatta ggtgctatag gtataaaatc gacaacatcg cgcaggaaaa aaacaggatg
     2761 gttttaatat cagtaacgta tactgtatac gatagaattt tggtaaagca tattttatga
     2821 ctcaaatagt tttattacat ggagcaatgg gaagttcatc gcaaatgcag ggtttgggaa
     2881 aacttcttga gcctcatttc gaagtttttg ctttcgactt ttgcggccat ggtgcgcgtg
     2941 cttcggaata tatttcgttt tcggtgcagg agtttgctgc cgatcttgac gagtttttga
     3001 tgcagaaaaa aataattaat ccctttattt ttggttacag tatgggaggc tatgtggcac
     3061 tttatcattc gttgcatttt aaaaatggtg caaaaaaaat atttacactt ggcacaaaat
     3121 ttaactggtc gccagctata gccgaaaagg aagttgcttt gctcgataca cacgtgatgc
     3181 aaactaaaat acccaaattt gtagaagtgc tgaagaacag gcatggcgaa aaaatgtggg
     3241 atgatgttgt taagaaaaca caggacatga tgcatctcct gggaaataat tatcagttgg
     3301 atgatgaaga cctggcaaac ataaaatgcc gtgttgtttt aatgcttgcc gatgatgata
     3361 atatggtaac cgaggaggaa accgaacaag cacatgatag tattgcgcac agcgaatatg
     3421 ttatcattca aaactcgaag catccaatcg aaaaagtgga tttggaagag ttggtaaagg
     3481 tgattttgaa gttttgattt ttttcaatgc attatttcgt ttaccgctgt attggatata
     3541 aaataaaaga ttacgtttag aaggaaggtt gtgtacgctc aaaaaaaatg gctttactac
     3601 tctaaggagt cagtatgtgt aatttgttat atttgccctc ctccaattta ataaaaacgt
     3661 ttaagtatat gagcaagaat cttttagatt ttgaaaaacc tataattgaa ttgcacgaac
     3721 agctcgaaaa agcaaagcaa atagccgaga aaggcaaagt tgataccggc caactgatac
     3781 gtgagttgga acaaaaaata gtaagcaccc gaaaagaaat atacgaacat cttacccctt
     3841 ggcaacgggt gcaggtttcg cgccatcccg agcggccata taccttgagt tatatcgaat
     3901 ttctgtgtgg aaaaacattt accgaattgt ttggcgaccg caccgtaggt gacgacaaag
     3961 ccatggtggg tggttttggt atgcttgatg atcgcacggt tatgtttatt ggtcaacaaa
     4021 agggtaacac taccaagcaa cgtcagtttc ggaattttgg aatgcccaat cccgagggtt
     4081 atcgcaaagc attgcggttg atgaaacttg ccgaaaaatt taacaagcct atagtaacct
     4141 ttatcgatac ccccggtgca tatccgggta tagaagccga agagcgtgga caaggtgagg
     4201 caatagcccg taacctttac gaaatgtcga ttcttaaagt acctgtaata tgtattatca
     4261 ttggtgaagg cgcatcgggc ggtgcattag gcattggcat tggcgataga gtgcttatgc
     4321 tcgaaaacac ttggtactca gtaatctctc ccgaatcatg ctcatctata ttgtggcgca
     4381 actgggacaa caaagagcgt gctgccgatg cacttaaact cactgctaac gatatgtttg
     4441 ccaatcattt aatagacgga attattcctg aaccagtagg tggcgcacat tcaaacaaag
     4501 aagaaatttt tgcaacagta aaaacatata tcataaacca ccttgacgaa ctgttgaaac
     4561 tcgacccgga aacccgcatt aagcaacgca ttgataagtt ttgcaaaatg ggtgtagtgg
//
LOCUS       DAORQA010000135         4613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4635_length_4613_cov_25.484642, whole genome shotgun sequence.
ACCESSION   DAORQA010000135 DAORQA010000000
VERSION     DAORQA010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4635_length_4613_cov_25.484642"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PK736_06930"
     CDS             <1..1098
                     /locus_tag="PK736_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRC33161.1"
                     /translation="AALISLIEFEHAVAENKGIGVMLAIVIGLMIGSISFSGSIIAFL
                     KLNGTLAKAIRLPSYNMLNNAFLLALFAMCAFMVFGTGASTMALYTLFAMSLLYGVLF
                     VIPIGGADMPVVISLLNSFTGLAAATGGFLYGNQAMLTGGILVGSAGTILTLAMCKAM
                     NRPLSNVIFGAFGESSGTESAGSSTMKGSIKDITTSDLAVLMNYSKKVIIVPGYGLAV
                     AQAQHVIHELEMILEERGVEVKYAIHPVAGRMPGHMNVLLAESNVDYGKLCEMEEVNP
                     EFTTTDVVLVVGANDVVNPAAKHDKASPIYGMPILDVENATNIIVNKRSMNAGYAGIE
                     NELFYNPKTSMLFGDAKKALTALVTELKSMA"
     gene            1125..1754
                     /gene="rsmG"
                     /locus_tag="PK736_06935"
     CDS             1125..1754
                     /gene="rsmG"
                     /locus_tag="PK736_06935"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010135176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRC33162.1"
                     /translation="MDSSAVIKKYFPQTSDKQIELFEKFAEELIEWNQKINLISRKDV
                     DNIFVNHILHSLTIAKYFSFADNTQILDIGTGGGFPGIPLAIIFPNCHFTLVDSIAKK
                     IVVVNDLIEKLQLKNVSAINERAEKLNIKVDVVVTRATASIDDLMKWCSPLFMRKGTM
                     PFRGLLALKGGDIEEECQNYTKYITINDLKKNFTEEFFETKKLVFLHVP"
     gene            1921..2079
                     /gene="rpmH"
                     /locus_tag="PK736_06940"
     CDS             1921..2079
                     /gene="rpmH"
                     /locus_tag="PK736_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRC33163.1"
                     /translation="MKRTFQPSNRKRNNKHGFRERMSTPNGRRVVSARRARGRKKLTV
                     ADETSRQK"
     gene            2607..>4613
                     /locus_tag="PK736_06945"
     CDS             2607..>4613
                     /locus_tag="PK736_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HRC33164.1"
                     /translation="MKKITTLVIWLCFAFLQVNAQCTTTNAKSCVCAQPGSLNCDLLP
                     DVMVATQPLQTIGTYGVMEYAYNDLSSKKSQLRVSVSTPNIGRGPLEVRATNTYVCGT
                     DTIIGTAPTVCVNTGLPPKQLLVQRVYRKEQDKMVYYERKAGSMTYHPTHGHMHVDDW
                     GIYTLRKSTSNPDPLTWPIITQGTKLAFCLMDYGYCSTYNQHCKDTNGTILTNLNTVN
                     YGLGGGAYSCSNVLQGISVGYLDIYYQYLDGMQMMLDSNICNGDYWLVVQIDPNNNFI
                     ESNENNNVVAVPFTLTKQRTRPDVQIVSGKTTICKGESVSFGANGASNYTWKASNGNS
                     TTMASALLVSPQVTTTYTVIGTNIGEGCSDTNYVTINVNNNPTVSISGNSTICPGQSS
                     NLTVSGAACYMWNPNINNNDSLATLVTATPSSTTTYNVTGINANGCSSTSSFTVNVSK
                     PTLSSNVSNVTICQTTSQAFVVTGASTYTWVPTTGLSSSTTSNVTCTPPYSTTYTVTG
                     TDAFGCTGTTSISVQILPNPYVSITPLATSYLNTVTSVALQGYPANGIFSGPGVSGSI
                     FNPYNLIPGSYVVKYEVTDNNGCKGQALATTSVAYSCSKPTGIVANNITATSAKLNWG
                     VASSGPQFKIKYKKNSSTIWNTKTINGNPHVVSTLLTGLDPNTVY"
BASE COUNT         1499 a          831 c          872 g         1411 t
ORIGIN      
        1 gccgcattaa tatccttaat cgaatttgaa catgctgtgg ctgagaataa aggaatagga
       61 gtgatgctgg caatagttat aggattaatg attggttcca tttctttttc gggaagtatt
      121 attgcatttt taaaacttaa cggaacactt gcaaaagcaa ttcggttacc aagttataat
      181 atgctgaata acgcatttct tttagcactt tttgcaatgt gtgcttttat ggtttttgga
      241 acaggtgcat ctacaatggc tttgtacaca ttatttgcta tgtcattatt atatggggtg
      301 ttatttgtaa ttccaatagg cggggcagat atgccagttg taatttcgtt attaaactct
      361 tttacagggt tggcagctgc cacaggaggg tttttatatg gtaatcaagc aatgcttact
      421 ggtggtatac tagtaggaag tgccggaacc attctgaccc ttgcaatgtg caaagccatg
      481 aacagaccat tgagcaatgt aatatttggc gcatttggcg aaagcagtgg taccgaatca
      541 gcaggaagtt ctactatgaa gggtagtata aaagatatca ccacaagcga tcttgcagtg
      601 ctgatgaact attccaaaaa agtaataatt gtgccaggat atggacttgc agttgctcag
      661 gcccaacacg ttattcacga attagaaatg atacttgaag agcgtggagt ggaagtaaaa
      721 tatgctatac atcctgttgc gggccgcatg ccggggcata tgaatgtatt acttgccgag
      781 tcgaatgttg attacggaaa attatgcgaa atggaagaag ttaatcctga gtttactaca
      841 accgatgtag tattagtagt tggggccaat gatgtagtaa accctgccgc aaagcatgat
      901 aaggcttcgc caatttatgg tatgccaatt ctggatgtag aaaatgcaac aaacataata
      961 gtaaacaaac gaagtatgaa tgccggttat gctggtatcg aaaacgagtt gttttataat
     1021 cctaaaacaa gcatgttgtt tggagatgcc aaaaaagccc ttaccgcatt ggttacagaa
     1081 ttaaagtcaa tggcttaatc ttaacctccc tttagattca attcatggat tcgtctgcag
     1141 ttattaaaaa atatttccct caaacgagtg acaaacaaat tgaacttttc gaaaaatttg
     1201 cagaagagct gattgaatgg aatcaaaaaa taaatctcat ttcgcgaaag gatgtcgaca
     1261 atatttttgt caatcacata ctgcatagcc ttaccattgc gaagtatttt tcatttgccg
     1321 ataatactca aatacttgac ataggcactg gtggcggctt tcccggaata cctctggcca
     1381 ttatttttcc caattgccac ttcacgcttg tcgactccat agccaaaaaa atagtggttg
     1441 taaacgatct tattgaaaaa ctgcaattga aaaatgtttc ggcaataaat gaacgagcag
     1501 aaaaattaaa tattaaagtt gatgttgttg taactcgtgc cacggcttct attgatgatt
     1561 tgatgaaatg gtgttcgcca ctatttatga gaaagggcac aatgccgttt cgaggtttgc
     1621 tagcgctaaa aggaggtgat atagaggaag aatgccaaaa ctatactaag tacataacta
     1681 tcaatgattt aaagaagaat tttacagaag aatttttcga aaccaagaaa ttggtctttc
     1741 ttcatgttcc ttaaaaatta agttattaac tttttgagtt ttatgttgta acgtattgat
     1801 aatcagtatg taaatatttt ttttcaaaac tttagtataa aaccctttta aaacgaagag
     1861 ttattattac atttgtcccc gtacttaatt attaaggaaa agtaaaaaaa taagttagaa
     1921 atgaaaagga cctttcagcc atcgaatcgc aaaagaaaca acaaacatgg tttccgcgaa
     1981 agaatgtcaa ctccaaatgg acgcagagtt gtatcggcac gcagagcacg cggccgtaaa
     2041 aaacttaccg ttgctgacga aacatctcgt cagaaataac atcgcaagat ttagttacgt
     2101 gtatatttaa gggctgtagt cttaattgac tgcagccttt ttttagtgtt ctacattgat
     2161 ctccattttt ttaattaagt ggaaaatggc attttgtatt tttttccgtt ttctaaaaca
     2221 ataccctcac tttacatttt aaatatttat ccggaatcga attcgcaatt ttatcaacta
     2281 attataattt tagttttact aaaagctaac cacaaaattt cgaagcagat attttaatat
     2341 tcatggctcc tataatgatt tttcttatga tttttgctat gattacttac aaatattttt
     2401 cacccaaaac cacataataa ttattcacca taataattaa tatttttacc aataattata
     2461 tgttgtatac ctatattatt ttgaacctac actaattttg gcatagttgg tctgtaaatt
     2521 gaaatggggg tattggtcat gtttatcttg aaaggattat ttgaaacatg aaccaacgta
     2581 actaacaaga ttaaaaattt tttgaaatga aaaaaattac cactttggtg atatggcttt
     2641 gttttgcctt tttacaagta aacgctcaat gcacaactac taacgcaaag tcatgcgttt
     2701 gtgcacaacc gggttcactt aattgtgacc ttttgcccga tgttatggta gcaactcagc
     2761 ctctacaaac tataggaact tatggagtaa tggaatatgc ctataacgat ttaagttcaa
     2821 aaaaatcgca actacgggta tcagtttcaa ctccaaacat tgggagaggc cctttggagg
     2881 ttcgtgcaac aaatacttat gtctgtggta cagacacaat tataggaact gctccaactg
     2941 tttgcgtcaa cacaggattg cctccaaagc aattgctggt gcagcgtgtc tatcgtaaag
     3001 agcaagataa aatggtatat tatgaacgca aagcaggttc gatgacatat catccaacgc
     3061 atggtcatat gcatgttgac gattggggta tttatacttt acgtaaatca acttcgaacc
     3121 ccgaccctct cacatggcca ataattactc aaggaaccaa acttgctttt tgccttatgg
     3181 attatggata ttgcagcact tacaaccagc attgcaaaga caccaatgga acaattctta
     3241 caaatttaaa tactgttaac tacggtcttg gtggcggtgc atattcatgc agcaatgtgt
     3301 tacagggcat atctgttggg tatctcgaca tttattatca atatcttgat gggatgcaaa
     3361 tgatgcttga ctcaaatatt tgtaatggcg attattggct tgtagtgcaa atagatccga
     3421 ataataattt tattgaatcg aacgaaaaca ataatgttgt tgcggtacca tttacattaa
     3481 caaagcaaag aacacgtccc gatgtgcaaa tagtaagcgg taagacaact atttgtaaag
     3541 gagaatcagt atcgtttgga gcaaacggtg cgagcaatta tacgtggaaa gcctcaaacg
     3601 gtaactcaac aactatggca tcagcgttac ttgtaagccc tcaggtaaca acaacgtata
     3661 ctgttatagg tacaaatatc ggggagggtt gcagcgatac gaattatgta actatcaatg
     3721 taaataataa tcctacagtt tctatttcgg gtaatagcac tatatgcccg ggtcaatcgt
     3781 caaatttaac agtaagtggt gcagcttgtt atatgtggaa tcctaacatt aataataatg
     3841 attcgcttgc tactcttgtt acagccactc cttcatctac taccacatat aatgtcacag
     3901 gaattaatgc taacggatgt tcctctacat catcgtttac agtcaatgta tcaaaaccaa
     3961 ctctatcatc aaatgtttcg aatgtaacta tatgtcaaac caccagccag gcatttgtag
     4021 ttactggagc ttctacttat acctgggttc caactacagg attaagcagc agcactacat
     4081 cgaatgtaac ttgcacgcca ccttacagca ctacgtatac agtaacaggt actgacgctt
     4141 ttggttgcac tggaaccacc agcatatcgg ttcaaatttt accaaaccca tatgttagta
     4201 tcacaccatt agcaacatct tatttaaata cagttaccag tgtagcgctg cagggatatc
     4261 cggcaaacgg aatttttagt ggtccgggag tgagtgggag catctttaat ccttataatt
     4321 taataccggg ctcttatgtt gtaaagtacg aagttactga taacaacgga tgtaaagggc
     4381 aagcattagc tactacttcg gtggcttatt cctgttccaa accaacaggt atagttgcga
     4441 acaatataac agcaaccagc gcgaagttaa attggggagt ggcgtccagc ggtcctcagt
     4501 tcaaaataaa atacaagaaa aattcctcca caatatggaa tacaaaaacc ataaatggta
     4561 atccacatgt agtaagcact ttacttacag gccttgatcc caatactgtt tac
//
LOCUS       DAORQA010000136         4608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4641_length_4608_cov_9.468922, whole genome shotgun sequence.
ACCESSION   DAORQA010000136 DAORQA010000000
VERSION     DAORQA010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4641_length_4608_cov_9.468922"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2865
                     /locus_tag="PK736_06950"
     CDS             <1..2865
                     /locus_tag="PK736_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33165.1"
                     /translation="TFNDAVAALNTSGVSGAVVFNVRNGTYTEQVLIGTITGTSAANT
                     ITFKSQSGDSSLVTLDFAAGGLSNYTLLLTGASYVSFKKLTIARTGINTAAAVVQMDN
                     GSNNCSFVNCVIKNDISLGSSNAQSLVYSPYLLTSIDNNNTFNNNRFVNGSYGIYWNG
                     HQTAFESGNAITNNIFENQTNTGIYSSANTLMLIEGNKIYSNSLSFGAGISNYLSGGN
                     NQIVRNQIDTIAQGVGIQSNTSTLTALNPDLIANNFVSIVGAAAGQANGILVAASSYI
                     NVYFNSVNIIAVVGTITNNNAFRISPTSANINVKNNIFHNKNGYVMNMSSGQAVSDYN
                     DLYKGAINNFAYWNNASQANFAAWQTASAGDANSLNLDPLFVSKGDLHITNYLLNGGA
                     LAGLGVANDIDNDLRDLINPDLGADEVLPPTFDIGIKSIFRPYDMACGETNAPVAVIV
                     KNYAFDKQTGFDVNIDITGFVTSNQTATYTDTIGPGEVDTIYFANTINTVSGGVLNIL
                     ATTLLGGDTQTQNDTLSLVATINPALAPPVPVVVAPVCIGDSTTVEVTPGQGGSILWY
                     SDPNATNFVGIGNPLSVLATANTTLYAKEFTGGTTGCLRITKVELADNPYDFIEIQNL
                     STANIDATGWVVAASNSYTDINLVNGTLWNLGNFNPGEIQFKTDNANTNYWGTNLLYN
                     TGSKSWAAIIDNNGNVVDIIFWDYTDAEIQNFNTNINGFNVTASLSWFGDGVNSSNCS
                     GQHLQRTGNIDLNDDGDWVCAFDAAGTQSATLITPFSDCGIGCASPVAAVLVAVYPAP
                     IVNLGNDTTIASPNTVTLDAGTGVSYLWSTGATTQTIVVSTSGTYTVTVTDANGCEGS
                     DVIVVNVLTALNEIVDGMIKNIYPQPATDVVNITFGNINMNGTLKFINSLGQVVVEKK
                     VDATMSNRKSSIDVSQLPAGWYSISLTDNKQIFNYKILLQ"
     gene            3018..>4608
                     /locus_tag="PK736_06955"
     CDS             3018..>4608
                     /locus_tag="PK736_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33166.1"
                     /translation="MNKNLTLVFICILFNLQFVNAQKQTSQWYFSNNSALDFLSGVPV
                     ALTNSAMNQYEGSSSIADDNGNLLMYTNGEQVWDATHTIMPNGTGLFGNNSTNQSALI
                     VPKPNSNTEYYVFTNDMTSLYYSVVDITLNGGLGAVTTKNIFLQGSISEKLAGVKHAN
                     GIDVWVMVHGSGNSTFYAFLVTPLSVAGTPVISTGGPIDPGTIGQMHFSPNGKFVAFS
                     SYGGAQNAAIFEFDNATGLASNGIGVPFSNQIYGCCFSPNSRYFYHQSNPPPCGVYQL
                     DLQAGNAAAILASSTQVGTGLSTNLGHMQIGYDKKIYLAHDGLQQLAVINYPDSPAAA
                     VDYNNLGVTLAGKTCGLGLPNFLASYFNDNVLIKNFCFGDSTTFSIEDTSALALAQWT
                     FDDPSSGPANVVTQYNTYHIFTAPGTYNVELIRVYNNALIDTAYYVVNIVTAPIINLG
                     ADTTLCIGQNIVLNAGSGSSFTWQNGSTLSTYTATVTGTYYVNVANIQGCVGNDSIDV
                     TFTTCAGPVAALASSDTLWCDK"
BASE COUNT         1410 a          873 c          952 g         1373 t
ORIGIN      
        1 acgtttaacg atgctgtggc agccctcaat acttccggtg tgtcgggtgc tgtagtgttt
       61 aatgtgcgca atggtactta taccgagcag gttttaatag ggacaattac gggcacatcg
      121 gctgccaaca ccattacttt taaatcgcaa agtggcgaca gttcgctggt aacgctggat
      181 tttgctgcag gtggccttag caattatacc cttttgctta caggtgcatc gtatgttagt
      241 ttcaaaaaat tgacaatagc acgtacgggg attaataccg ctgccgctgt tgtgcaaatg
      301 gataatggaa gcaataattg cagttttgtc aattgtgtta ttaaaaacga tatttcgttg
      361 ggaagcagta atgcacaatc gttggtttac tcaccatact tacttacttc aatagataat
      421 aataacacct tcaacaacaa tcgttttgta aatggaagtt atggcattta ttggaacggg
      481 catcaaactg cattcgaaag cggaaatgcc atcaccaaca atattttcga aaaccaaaca
      541 aacacaggaa tctattcaag tgctaacaca ttgatgctta tagaaggaaa taaaatctat
      601 tcgaacagtt taagttttgg agccggcatc agcaattatt tgagtggtgg taataaccaa
      661 attgtacgta accaaattga tacaattgcg caaggtgtgg gaattcaatc taacacatca
      721 acactcacgg ccttaaaccc cgatttgatt gctaataatt ttgtttctat tgttggtgca
      781 gctgccggtc aggccaatgg aatattagtg gccgcgagct cgtatatcaa tgtatatttc
      841 aattcagtaa atattatcgc agttgttgga accattacca acaataatgc attccgcatt
      901 tcgccaacga gtgcaaacat caatgttaaa aacaacattt ttcataacaa gaatggttat
      961 gtaatgaata tgtcttcagg ccaggccgtg agtgattata atgatttgta caaaggtgcc
     1021 attaataact ttgcttattg gaacaatgct tcacaagcca attttgcagc atggcaaaca
     1081 gcttctgcgg gcgatgccaa ttcattaaat ttagatcctc tgttcgtgtc gaaaggcgat
     1141 ttacacatta ccaattattt gctcaatggt ggtgcattgg ctggtcttgg tgttgccaac
     1201 gatatcgaca atgatttgcg cgaccttatc aatcctgacc ttggtgccga tgaagtattg
     1261 ccaccaactt ttgatatagg tataaaatct attttccgcc cgtatgatat ggcttgtggc
     1321 gaaaccaatg cgcctgttgc tgtaatcgta aaaaattatg ctttcgataa acaaacaggt
     1381 ttcgatgtaa acattgatat caccggtttt gtaacatcga atcaaacagc aacttataca
     1441 gataccattg gccccggtga ggttgatact atttattttg caaatacaat caatacagta
     1501 agtggtggtg tattaaatat tcttgcaaca acacttttgg gtggcgatac acaaacccaa
     1561 aacgatactt tgagcctcgt agcaactatt aacccggccc tggcaccacc cgtgcctgtg
     1621 gtagttgctc cggtttgtat tggtgatagt acaacagttg aagtaactcc cggtcagggt
     1681 ggttctatat tgtggtacag cgatcccaat gctacaaact ttgttgggat tggcaatcct
     1741 ttaagtgtat tggcaacagc aaatacaacg ttgtatgcca aagagtttac aggtggcaca
     1801 actggttgtt tacgcattac aaaagtggaa ttggccgata atccgtatga tttcattgag
     1861 atacaaaacc tgagtacagc aaatattgat gctacaggtt gggtggttgc tgcaagtaat
     1921 agttatacag atatcaatct tgttaatgga acattgtgga atttgggaaa ttttaacccc
     1981 ggagaaatcc aattcaaaac agataatgcc aatacaaatt attggggtac gaatttgctc
     2041 tacaatacag gaagtaaaag ttgggctgca attattgaca ataacggtaa tgtagttgat
     2101 attatatttt gggattacac cgatgccgaa atacaaaact tcaatactaa tataaacggt
     2161 tttaatgtta ctgccagctt aagttggttt ggcgatggtg ttaattcatc taattgcagc
     2221 ggacaacact tacagcgcac aggaaatata gacttgaatg atgatggtga ctgggtgtgt
     2281 gctttcgatg ctgcaggaac ccaaagcgct actttgatta ctccgttttc tgattgtggt
     2341 ataggttgtg ccagtccggt tgcggcagtg cttgttgcag tttatcctgc gccaatagta
     2401 aatctaggta acgatactac tatagcttcg ccaaacactg taacattaga tgcaggcaca
     2461 ggggtaagtt acttatggtc gacaggtgct actacccaaa caatagtagt aagtaccagc
     2521 ggtacgtata ctgttaccgt taccgatgcc aatggctgcg aaggcagcga tgtgatagtg
     2581 gtgaatgtgt taacagccct gaatgaaatt gttgatggaa tgataaaaaa catttatcct
     2641 caacctgcaa ccgatgtggt gaatattacc tttggaaata tcaatatgaa tggcacattg
     2701 aaatttataa atagtctggg tcaggtagta gtggagaaga aagtagacgc tacgatgagc
     2761 aaccgcaaat catcaattga cgtttcacaa ttgcctgcag gatggtacag cattagcctt
     2821 accgacaaca aacaaatttt caattataag attttgttgc aataattatt tgaaccaaaa
     2881 ttaagacgat aaacaaaagc cgctaaaaag cggcttttgt gttttatata attgtcattt
     2941 ttattttaag gttgtttgta tgataaataa attactacat ttgaaatcaa taaatctaac
     3001 taaacattta aaacaaaatg aataaaaact taacgcttgt atttatctgc attctcttca
     3061 atttgcaatt tgtgaatgcg caaaaacaaa ccagccaatg gtattttagc aacaacagcg
     3121 cactcgattt cctcagcggt gttccagttg ctcttaccaa tagtgccatg aatcagtatg
     3181 aaggcagttc atctattgct gatgataatg gcaacctgtt aatgtataca aatggtgaac
     3241 aggtatggga tgcaacacat acaataatgc caaatggcac aggattgttt ggcaacaaca
     3301 gcaccaatca atcggcactc atagttccaa aaccaaatag caataccgag tattatgttt
     3361 ttaccaacga catgacaagt ttatattatt cggttgtcga tatcacttta aatggtgggc
     3421 ttggagcagt gaccacaaaa aatatttttt tacagggcag cattagcgaa aagcttgccg
     3481 gtgtaaaaca tgccaatgga atagatgttt gggtaatggt tcacggttcc ggcaatagca
     3541 cattttatgc gtttttggtt acgcctttaa gcgttgcagg tacaccagta atttctacag
     3601 gaggccccat cgatccggga acaattgggc aaatgcattt ttctcctaat ggaaaatttg
     3661 tggctttcag tagttatggt ggcgcccaaa atgcagcaat tttcgaattc gataatgcaa
     3721 ccggtttggc atcgaatggt attggtgttc ctttttcaaa tcaaatttat ggatgttgct
     3781 tttcgcccaa tagccgatac ttttatcatc aatcgaatcc accaccttgc ggggtttatc
     3841 agctcgattt gcaagccgga aatgccgcag caattcttgc atcatcaaca caagtgggta
     3901 caggtttatc tacaaacctt ggacacatgc aaataggtta cgataaaaaa atatacctgg
     3961 cacatgatgg attgcagcaa ttagcagtaa taaattatcc tgattctcct gctgcagcgg
     4021 ttgattataa taaccttggt gtaacgcttg ccggcaaaac ctgtggactg gggctgccta
     4081 attttttagc aagttatttt aatgataatg tacttataaa aaacttttgc tttggcgatt
     4141 ctacaacttt ctccattgaa gatacttcag cattagcact ggctcaatgg actttcgatg
     4201 acccttcatc gggcccggca aatgtggtta cacaatacaa cacataccac atttttacag
     4261 cacccggcac atataatgtt gagcttataa gggtttacaa taatgcactt atcgacacag
     4321 catattatgt ggttaatatt gttactgcac cgattattaa tcttggtgcc gataccactt
     4381 tgtgcatagg gcaaaatatt gtgctcaatg caggtagtgg aagttcgttc acatggcaaa
     4441 atggctccac actttctacg tatacagcca ctgttacagg aacttattat gtaaatgtag
     4501 caaatataca aggctgcgtt ggcaacgatt cgatagatgt aacttttaca acctgtgcag
     4561 gccctgtggc ggcattagca tcaagcgata cattgtggtg cgataaaa
//
LOCUS       DAORQA010000137         4607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4642_length_4607_cov_7.774824, whole genome shotgun sequence.
ACCESSION   DAORQA010000137 DAORQA010000000
VERSION     DAORQA010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4642_length_4607_cov_7.774824"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /gene="murD"
                     /locus_tag="PK736_06960"
     CDS             complement(<1..978)
                     /gene="murD"
                     /locus_tag="PK736_06960"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRC33167.1"
                     /translation="MEKAKKIIVLGGGESGVGAAVLAVAKGFDVFVSDKGAIKQNYKD
                     KLIQCNIAFEEGKHSYDLFENAIEVIKSPGIPNHAEAVKYFRDKNIPVVSEIEFAARY
                     THAKKICITGTNGKTTTTMLTYHILQKAGYNVGLAGNVGTSLALQVTENNFDYYVIEL
                     SSFQLDDMFDFKADIAVMLNITPDHLDRYEYSLQKYIDSKFRILQNMTADGAFVYCDD
                     DATIKQQLIRKLPPCNLYPFSLKRSENVNAWVEDEKLHVTIKSKQLIMSIFEIAIHGK
                     HNLYNSMAASIPAFLLDIKDETIRESLMDFQNIEHRLEFVNSVNGVDYIN"
     gene            complement(1003..2253)
                     /gene="mraY"
                     /locus_tag="PK736_06965"
     CDS             complement(1003..2253)
                     /gene="mraY"
                     /locus_tag="PK736_06965"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRC33168.1"
                     /translation="MLYYLFEYLNHHFDFPGAGVFKYISFRASLAFIASLLFALFIGK
                     RLIVVLQNKQVGETIRDLGLEGEAQKKGTPTMGGLIILAALLLPTVLLARLDNVYIIT
                     MIITAVWCGMIGFIDDYIKVFKKDKAGLAGRFKVMGQIGLGLIVGCILYFNQFVVVRE
                     KVPAAPIDNLSAFPADFANNPTATYVAKDVKSTITTIPFIKGHEFDYASLLKWTGKNY
                     RDYAWLAFIPIVIFIITAVSNGANITDGIDGLATGTSAIIGSALAVLAYVSGNTVFAN
                     YLNILYLPNVGELMVFSGAFIGACVGFLWYNSYPAQVFMGDTGSLALGGIIAAFAISI
                     RKEFLIPILCGIFLIENISVIMQVAYFKYTRKKYGEGRRIFRMSPLHHHYQKLNYHES
                     KIVTRFWIIGIMLAALSVITLKLR"
     gene            complement(2253..3716)
                     /locus_tag="PK736_06970"
     CDS             complement(2253..3716)
                     /locus_tag="PK736_06970"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRC33169.1"
                     /translation="MKLLKDILYKVAITEVYGSTDMFINDVTFSTDAVAESDVFVAIK
                     GLKFDGHSFIESAIKQGCAAVICESLPVNIVKGITYVKVKSSREALAYMSANFFNNPS
                     ERIKLIGVTGTNGKTTTVSLLHNLFAESGKKCGLISTVGILIGNKKIEATHTTPDAYQ
                     LNKLLNDMVVDGCEYCFMEVSSHAVVQHRVTGIHFTGGVFTNITHDHLDFHITFAEYL
                     KAKKGFFDMLPETAFAIVNNDDKNGLVMLQNCRARKITYSIRTDSNFKCRIIENSFGG
                     LMLQIKHHEILFRLIGSFNAYNITSVFAVAMELGLDEMTTLIILSKLSAPEGRFQYTI
                     SANKVTGIVDYAHTPDALENVLSTIKDIRNGNEKIITVVGCGGDRDAAKRPLMARIAC
                     DFSDKVILTSDNPRSEDPNDILKQMQEGVPVKHAKKTLSIADRGEAIKAACSMATKGD
                     IILIAGKGHEKYQEIKGVKNPFDDMQVLVESLNLFEN"
     gene            complement(3743..>4607)
                     /locus_tag="PK736_06975"
     CDS             complement(3743..>4607)
                     /locus_tag="PK736_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRC33170.1"
                     /translation="WSGATLPVMSYGYETKLTPLQTLTFYNAVANNGRMVKPIFVQEI
                     RKQGKLIHTYETEVVNAKICSDATLKQAREMMEGVVLRGTATNLKFDTYTIAGKTGTA
                     KMAFGKSGYSLSDSVRYQSSFCGYFPADKPLYSCIVVMYDLSPKFYYGNVAAGPVFKE
                     IADKVYSTSIAMHNEIKTDSLINKKQPEYKGGDITALNKVLQSVNKKPVENKNKFYAS
                     VNNNTSGVSVNLFNGLVPDVAGMPMRDAIYILESTGMYVTPVGKGVVKKQSVQAGTPI
                     VKGKKIIIELS"
BASE COUNT         1360 a          948 c          800 g         1499 t
ORIGIN      
        1 gtttatatag tctacaccat tcacagagtt tacaaactcc agtcggtgtt ctatattttg
       61 aaaatccatc agactttcgc ggatagtctc atccttgatg tccaataaaa aggcaggaat
      121 agatgctgcc attgaattgt acaaattgtg cttgccatga atggcgattt caaaaattga
      181 cataattaat tgttttgatt ttattgttac gtgtagtttc tcatcttcga cccatgcatt
      241 aacattctcg ctgcgcttga gcgaaaacgg atacaaattg catggaggta attttcttat
      301 tagttgttgc ttaatagttg catcgtcatc gcaataaacg aatgcaccat cagctgtcat
      361 gttttgcaga atgcggaatt tagaatcaat atatttttgc aaagaatatt cgtagcgatc
      421 taaatgatcg ggagtaatat tgagcatcac agcaatgtca gctttgaagt caaacatatc
      481 gtcaagctga aaactgctga gttcgattac ataataatcg aaattatttt ctgtaacctg
      541 cagcgccaac gaagttccta cattgcctgc caatcctaca ttgtagcctg ccttttgcag
      601 aatgtggtaa gtaagcatgg ttgtggttgt tttgccattg gtaccggtaa tacatatttt
      661 ttttgcgtga gtgtatcgtg cggcaaactc tatttcacta acaaccggaa tatttttatc
      721 acgaaaatac tttaccgctt ctgcatggtt tggtatgccc gggcttttta ttacttcaat
      781 ggcattttca aataaatcgt acgagtgttt gccttcttca aaagcaatat tgcattgtat
      841 cagtttatcc ttatagtttt gtttgatagc acctttgtcc gaaacaaaaa catcaaagcc
      901 tttggcaaca gcaagcactg cagcaccaac gccactttcg cctccaccta atactatgat
      961 tttctttgcc ttttccattt tgtacgtata cgcttatagt tattatcgca gttttaatgt
     1021 tatcacactt aatgctgcga gcataattcc aataatccaa aaccttgtta caattttgct
     1081 ttcgtgataa ttgagttttt gataatgatg atgtaaaggc gacattctaa aaatgcgtct
     1141 gccttcacca tattttttac gggtgtattt gaagtatgct acttgcatta ttaccgaaat
     1201 attttcgatg agaaatattc cacacagtat tggaataaga aattctttgc ggatagaaat
     1261 ggcaaaagct gcaataattc cgcccagtgc aaggctgccg gtatcgccca taaaaacctg
     1321 tgccgggtat gaattgtacc aaaggaaacc aacacatgca ccaatgaatg caccgctgaa
     1381 aaccattaac tcgcctacgt ttggcaagta caaaatgtta agataattgg caaacactgt
     1441 gttaccactt acgtatgcca acacagccag tgccgagcca atgatggccg aagttccggt
     1501 agcaagccca tcgataccat cagtaatatt tgcaccattc gaaacagcag taataataaa
     1561 aatgacgata ggtataaaag caagccatgc ataatctcta taatttttgc cagtccattt
     1621 tagtaacgat gcataatcaa attcatgtcc tttaataaat ggaatagtgg tgatggtaga
     1681 ttttacatct ttggcaacat aagttgcggt aggattattt gcaaagtccg caggaaaggc
     1741 actaaggtta tcgataggtg ctgcaggaac tttttcgcgc acaacaacaa attgattgaa
     1801 atataaaatg caacctacaa tcaagcccaa cccaatttgc cccatcacct tgaaacgtcc
     1861 ggccaatcct gctttgtctt tcttgaatac ttttatgtag tcatcaataa acccaatcat
     1921 tccgcaccac acagcagtaa ttatcatggt gatgatgtat acattatcga ggcgtgctaa
     1981 taatactgtg ggtaataata gagctgccag aataattaag cctcccatgg ttggggtacc
     2041 ttttttttgt gcttcgcctt caagtcctaa gtcgcgaatg gtttctccta cttgtttatt
     2101 ttgcagaact acaatcagcc ttttaccaat aaataaagcg aacaataatg acgcaataaa
     2161 tgcaagcgat gcacgaaacg aaatgtactt aaacaccccg gctccaggaa aatcaaaatg
     2221 atgattcaga tattcaaaca ggtaatacaa cattagtttt caaacagatt taaagattct
     2281 actaaaactt gcatatcgtc aaagggattt tttacgcctt ttatttcctg atatttttcg
     2341 tggcctttac ctgctattaa aataatatca cccttggttg ccatactgca cgcagccttt
     2401 atagcttcgc ccctatcggc aatggataat gtttttttcg catgttttac aggcacacct
     2461 tcctgcattt gtttcaaaat atcgttggga tcttcgctgc gggggttatc ggaagtgagt
     2521 atcactttat cgctgaagtc gcaggcaatg cgtgccataa gggggcgctt ggctgcgtct
     2581 ctatcgccac cacatcctac tacggttatt attttttcgt ttccattacg aatatctttg
     2641 atagtgctta atacattttc caatgcatca ggagtatgcg cataatcaac aatgcctgtt
     2701 actttattcg ctgaaatggt atattgaaaa cgtccttcgg gtgcacttaa tttcgatagt
     2761 ataataagcg tagtcatttc atcgagaccc aattccattg ccacagcaaa aacacttgta
     2821 atattatatg cattaaaact tccaattaac cggaataaaa tttcgtgatg ttttatttgc
     2881 aacatcagtc caccaaaact attttcgatg atgcggcatt taaagttgct gtcagttctt
     2941 atagagtagg tgatttttcg cgctctgcaa ttttgcaaca ttaccagacc atttttatca
     3001 tcgttgttca caatggcaaa tgcagtttcg ggcaacatat cgaagaaacc ttttttagct
     3061 tttaagtact cggcaaatgt tatgtgaaaa tcaagatgat catgtgttat attggtaaat
     3121 acacctccgg taaaatggat tcctgtaaca cgatgttgaa ctacagcatg tgaacttact
     3181 tccataaaac agtattcaca tccatctaca accatatcat tcagcaactt gttaagttga
     3241 tatgcatcgg gagtagtatg tgtagcttct atttttttat tgccaatcag tattccaaca
     3301 gttgatataa gtccacattt ctttccgctt tctgcaaata aattatgcaa caaactaacc
     3361 gttgttgttt taccatttgt accggtaact ccaataagtt tgattctttc agaagggtta
     3421 ttgaaaaaat ttgccgacat atatgccaat gcctcgcggc tgctttttac ttttacataa
     3481 gtaattcctt ttacaatatt taccggaaga ctttcgcaaa taacagcggc acaaccttgc
     3541 ttaatagcac tttcaataaa cgaatgcccg tcaaacttta atccctttat tgctacaaac
     3601 acatcgcttt cggcaacggc atcagtacta aatgtgacat cgttgataaa catatcagta
     3661 gaaccataca cttcggtgat ggcaacttta tataatatgt ctttaagcaa tttcatatcc
     3721 ggtgcttcgt atattgttat agttagctta gctcgattat tattttttta cctttaacga
     3781 taggcgttcc agcttgtact gattgttttt ttacaacgcc ttttccaacc ggagtaacgt
     3841 acatgccagt gctttcgaga atgtaaatgg catcacgcat gggcatccct gccacatcgg
     3901 gcacaagtcc attaaacaaa ttgaccgaaa cacccgaagt attattattt accgatgcat
     3961 aaaatttatt cttattctca accggttttt tatttacact ctgcagtact ttattcagtg
     4021 ctgttatgtc accaccttta tattcaggtt gctttttatt aataagtgaa tctgttttta
     4081 tttcgttatg cattgcaatg cttgtagaat aaactttgtc ggcaatctct ttaaacacag
     4141 gacctgcagc aacattgcca taataaaact tgggcgataa atcatacatt acaacaatgc
     4201 agctgtataa gggcttatcg gcaggaaagt atccacaaaa agaagattgg tatcgaaccg
     4261 aatcacttaa tgaataaccg cttttaccaa atgccatttt tgctgttcct gtttttccgg
     4321 ctattgtgta cgtatcaaac tttaaattgg tggcagtgcc tctaagcact acaccttcca
     4381 tcatttcgcg cgcttgcttg agtgtggcat cggaacaaat tttagcattc actacttcgg
     4441 tttcatatgt gtgtataagc ttaccctgct tacgaatttc ctgcacaaaa attggcttta
     4501 ccattcttcc attatttgca acagcgttat aaaatgtaag cgtttgcaat ggagtgagtt
     4561 ttgtttcata cccataactc attactggca aagtagcacc actccat
//
LOCUS       DAORQA010000138         4583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4675_length_4583_cov_12.945892, whole genome shotgun sequence.
ACCESSION   DAORQA010000138 DAORQA010000000
VERSION     DAORQA010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4675_length_4583_cov_12.945892"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..2310)
                     /locus_tag="PK736_06980"
     CDS             complement(25..2310)
                     /locus_tag="PK736_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC33171.1"
                     /translation="MKKQHLIALLFIFCIHKGWSQKEGNQWFFATYCGMDFNSGAPVN
                     VPGGQTNTSEGSASVADGNGNLLFYTDGITIWNKNHLAMPNGFALGGGISSTQSAVIL
                     KLPGSANLYYVFTVNENFGTIGYSIVDMTLQGGNGDVTTKNTFIASNVAEKICAIRHA
                     NNIDWWIIVHNNTINEYSAYLLTSAGLTLTPVNSVGLNLTSNSFIGYLEANGAGTTIA
                     AAFWNDVVEFQIYDFDDATGTITNPLTMPYHPYSSGAYGVEFSPSGQYLYTTLITPGE
                     VRQYDLWAGNNAAILASEVLVGTCSNSFNGGMSLAPDGKIYIAQYGIKYLAVINNPDA
                     PGLACNFVDLGYNLGVGTGSLGIPNFPSDIFSLKINASPLCFGDTTIFSIDDSTGYVA
                     ISWDFGDPGSGNNSAYTYLASHIFTSAGTYNVTVIVVYANSVSDTLNYPVTIELPPVI
                     NLGVDTALCNGQTLTLNAGSGYASYMWQNSSTLSTYTATVGGTYFVEVASAAGCKGAD
                     SIDVTFSACAGPLAALSSSDTLWCDKTCIDFFDLSQNNPTSWTWYFQGASPSTSTDQN
                     PTGICYNNYGSFDVTLVACNAAGCDSLYLNDFVTEFQLPLPPTITYSNDTLYASPAFS
                     YQWFNTNNVNLLLSTNNYFVPVVDGIYFVLGFDSNGCATPSTTFGFYTGINNYTYNSN
                     DVILTPVDGGNYFSLQIISNQHLKNLSLIDVAGKQINLTNYNADGDRKFILDLNNVNK
                     GVYFVKVEFDNYSSIRRLIKF"
     gene            complement(2330..>4583)
                     /locus_tag="PK736_06985"
     CDS             complement(2330..>4583)
                     /locus_tag="PK736_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33172.1"
                     /translation="VILMAILFFNEAQAQKETWNWYFGNQCALNWSTGVPVAMTNSAM
                     NQYEGSASISDANGNLLFYTDGISAWNTNHITMPNGTGMMGNFSTNQSALIVPKPGST
                     TEYYLFSNSSFELYYSIVDMSLQGGLGDVTTKNVFMKTNVAEKLAGVRHSNGAAYWVM
                     VHEPGNNSFFAYEVTAAGVSTTPVISSVGINDPGSIGQMQFSPDGKRVAFGTYSSTTN
                     LGIFDFDAATGIVSNPINVPVPNQVYGLSFSPNSKVLYRSSNPFAGQLFQYDLDAGNA
                     AAVIASELMIANGFSNDEGHLQIGPDKKVYLARDGLSTLGVIEYPDSVGMGCAWNPNG
                     VTLAGKLCGLGLPNFVASYFNKSALIEHFCFGDTTTFLIADSASYLVTEWNFDDPTTG
                     PANISYNFSTSHVFSAPGTYNVQVITVNINSFVDTALYTVVIEASPVINLGNDTTLCT
                     GQSITLNAGAGYASYLWQNGSSTSTYTATVSGTYGVQVSSAAGCKGIDSIDVTFSPCA
                     GPVAALASSDTLWCDKTCIDFFDLSQNNPTSWTWYFQGASPSTSTDQNPTGICYNNYG
                     SFDVTLVACNAVSCDSLFLDDFVTEFQLPAPAVITFINDTLFASPAVAYQWYNINNIN
                     VVLSTNNYFVPVVDGGYFVLGFDSIGCSVPSATFGFYTSLYDNNPSHANVYYNSYNHN
                     IEIKYNELNGENVNYVLLNAIGQPVETIVQLSSTGTNTINTNNLTEGIYFLQITDGIK
                     TTTFKLSIVK"
BASE COUNT         1422 a          941 c          897 g         1323 t
ORIGIN      
        1 atatttattt acgaaaaaac tatattaaaa ttttatcaac ctgcgtatac tgctatagtt
       61 atcgaattct acttttacaa aatacacgcc tttgtttaca ttattcaaat caagaataaa
      121 ttttctgtcg ccatcggcat tatagtttgt aagattgatt tgttttcccg caacatcaat
      181 tagtgataaa tttttcaggt gttggtttga aattatttgt aaactaaaat agttgccgcc
      241 atcaactgga gtaagtatta catcgttact gttataagta taattattta ttcctgtgta
      301 aaaaccaaat gtggtagaag gagtagcgca tccattgcta tcgaaaccta aaacgaaata
      361 aataccatcc actacgggca caaaataatt gtttgtgctc aataaaaggt ttacattatt
      421 cgtattgaac cattgataag aaaaagccgg agaggcataa agcgtatcgt tactgtaggt
      481 aatagttggt ggcagtggca attgaaattc agttacaaaa tcattcaggt ataatgagtc
      541 gcacccggca gcattgcaag ctacaagcgt aacatcgaaa ctgccatagt tgttgtagca
      601 aatcccagta gggttttggt cggtagaagt agaaggcgaa gcaccctgaa aataccatgt
      661 ccatgatgta ggattgtttt gcgagaggtc gaaaaagtcg atgcaggttt tatcgcacca
      721 aagtgtatcg ctcgatgaca gtgctgccaa tggccctgca caagccgaaa aagtaacatc
      781 tatcgaatca gctcctttgc atccggctgc agatgctact tctacaaaat atgttccgcc
      841 aacggtagct gtatacgtac ttaaggttga tgaattttgc cacatgtacg aggcataacc
      901 actacctgca tttaatgtaa gtgtttgccc attgcacaag gcggtatcaa ctccaaggtt
      961 tataacgggt ggcagctcta tggttaccgg gtaatttaac gtatcggaaa cggaattggc
     1021 ataaaccaca ataacggtaa cattatacgt accggcagaa gtaaagatgt gcgatgcaag
     1081 ataagtgtaa gcagaattgt tgccgctgcc cgggtcgcca aaatcccacg atatagctac
     1141 ataacctgtt gagtcgtcaa tcgaaaaaat ggtagtatcg ccaaaacaca aaggcgaagc
     1201 atttattttt aaagagaaaa tatcagaagg gaaatttggt attcccaatg agcctgtgcc
     1261 gacacccaaa ttatagccga ggtcaacaaa attacaagca agccccggag catcgggatt
     1321 atttatcaca gccagatatt taataccgta ttgtgcaatg taaattttgc catcgggtgc
     1381 aagcgacata ccaccattga acgaattgct gcaagtaccc accaatactt ccgaagcaag
     1441 tattgccgca ttgttacccg cccacaaatc gtattgacgt acttcgccag gagtaattaa
     1501 agtggtatat aaatattgac cgcttggcga aaactctaca ccataggcgc cacttgaata
     1561 tggatggtaa ggcatagtga gcgggttggt aatagtaccg gtggcatcat caaaatcata
     1621 aatttgaaat tcaacaacat cgttccaaaa tgccgcggca attgttgttc ctgcaccatt
     1681 ggcttcgagg tagccaataa aactattact tgtgaggttc aatcctacac tatttacagg
     1741 ggttaaggtt aggcccgctg atgtaagaag ataggcactg tattcattta tagtattgtt
     1801 gtgaactatt atccaccagt cgatattatt tgcatgtcgt attgcgcata ttttttcggc
     1861 aacattgctg gcaataaaag tgtttttggt tgttacatca ccattgcccc cttgcaatgt
     1921 catatcaaca atggaatagc ctatggttcc gaaattttcg ttcacggtaa aaacgtaata
     1981 caaatttgca ctgccaggca gcttcaaaat aacggcagat tgtgtgctcg aaattcctcc
     2041 gccaagtgca aaaccattgg gcatagccaa atggttttta ttccatattg taatgccatc
     2101 ggtataaaac aacaagttac catttccatc ggccacactt gctgaacctt cacttgtgtt
     2161 ggtttgtccg cctggtacat tcacgggtgc tccgctattg aagtccatgc cgcaataggt
     2221 tgcaaaaaac cattgattgc cttctttctg actccatcct ttatgaatac aaaaaataaa
     2281 gagtaatgct attaaatgct gttttttcat gatgttttat ttattaatat tattttacta
     2341 tacttaattt aaatgttgtg gtttttattc cgtctgttat ttgtagaaaa taaatgcctt
     2401 cggtaaggtt gtttgtgttt attgtgtttg ttcccgtact gcttagctga acaattgttt
     2461 ccaccggctg ccctattgca ttcaataaaa cataattaac attctctcca tttaattcat
     2521 tatattttat ttctatattg tggttataac tattgtagta tacgttagca tgcgatgggt
     2581 tgttatcata aagtgaagta taaaaaccaa aagtagccga aggtaccgag cagccaatgc
     2641 tatcaaaacc caaaacaaaa taaccaccat ccactacagg cacaaaataa ttattcgtgc
     2701 tcagcaccac attaatatta ttgatattat accattgata agcaacagcg ggcgaagcaa
     2761 acaaagtatc gtttataaaa gtaattacag caggtgccgg tagttgaaac tctgtaacaa
     2821 aatcatcaag gaacaatgag tcgcacgaaa cagcattgca ggcaaccaaa gtaacatcga
     2881 agctgccgta attattataa caaatccctg ttgggttttg gtcggtagag gtagaaggcg
     2941 aagcaccctg aaaataccat gtccatgatg taggattgtt ttgcgagagg tcgaaaaagt
     3001 cgatgcaggt tttatcgcac cacaatgtat cgctcgatgc taaggcagca acagggccgg
     3061 cacaaggcga gaatgttaca tctatcgaat cgatgccttt acatcctgct gccgaactta
     3121 cctgtacacc atatgtaccg ctaacggtag ccgtatacgt tgatgtagaa cttccgtttt
     3181 gccaaaggta tgatgcatat cctgctccgg cattcaacgt aattgattgc cctgtgcaca
     3241 gcgtagtatc gttgccaaga tttataaccg gtgatgcttc aattaccact gtataaagcg
     3301 cagtgtcgac aaaggagtta atatttacgg taatcacctg tacattataa gtacccggtg
     3361 ccgaaaaaac atgtgatgta gaaaaattgt atgatatgtt tgccgggccg gttgtaggat
     3421 catcaaagtt ccattcggta acaaggtatg atgccgaatc ggcaatcaaa aatgttgtgg
     3481 tatcaccaaa acaaaaatgc tcaatcaatg cagatttatt aaaatacgag gctacaaaat
     3541 tgggaagacc cagcccacac aatttacctg cgagtgttac accatttgga ttccatgcgc
     3601 aacccattcc caccgaatcg ggatactcaa taacacctaa agttgataac ccatcgcgtg
     3661 cgagataaac ttttttatcg gggccaattt gtaaatggcc ttcatcgttg ctgaatccat
     3721 tagcaatcat caactccgat gcaatcactg ctgcggcatt tccggcatcg agatcatatt
     3781 gaaataattg gcctgcaaaa ggattactgc tgcgatacaa caccttactg tttggagaaa
     3841 agcttaatcc ataaacttgg ttaggcaccg gcacatttat agggttcgaa actattcctg
     3901 tggcagcatc aaaatcaaaa attcctaaat tggtggtgct tgaataagta ccaaaagcaa
     3961 cacgtttgcc atcgggcgaa aactgcattt gtccaatgct acccggatcg tttataccta
     4021 cactactaat aaccggtgtg gttgaaaccc ctgcagctgt aacttcatat gcaaaaaaac
     4081 tattgttgcc gggctcatga accatcaccc aatatgcagc accattgcta tgacgaacac
     4141 cggcaagttt ttcggcaaca ttggttttca tgaaaacatt ttttgttgtt acatcgccca
     4201 ggccaccttg caaactcata tcaactatgg aataatataa ttcgaaacta ctgttgctga
     4261 acaaataata ttcagttgta cttcccggtt tgggaacaat caatgcagac tgattggtac
     4321 taaagttacc catcattcct gttccgtttg gcatggtaat atgattggta ttccatgcac
     4381 taattccatc ggtataaaaa agtaaattcc catttgcatc ggaaatagaa gcactgcctt
     4441 cgtattgatt cattgcactg ttggtcattg caacgggtac acctgtgctc cagttaagcg
     4501 cgcattgatt tccaaaatac cagttccacg tttccttttg tgcttgtgct tcattaaaaa
     4561 ataaaatggc cataagaatg acc
//
LOCUS       DAORQA010000139         4572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4685_length_4572_cov_8.674563, whole genome shotgun sequence.
ACCESSION   DAORQA010000139 DAORQA010000000
VERSION     DAORQA010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4685_length_4572_cov_8.674563"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1963
                     /locus_tag="PK736_06990"
     CDS             <1..1963
                     /locus_tag="PK736_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HRC33173.1"
                     /translation="YYKNVKVLHGHFKIHQRADGMVQTANGNIITGLNLDLTTTVNEN
                     EALANALSFMNAKKYLWQNEVSENELKRTENNVNATYFPKGELVFAPANYDVTYLAAD
                     YRLTWQFTIYSDDINVAAKCVFVDALSGKVVYHYDISMSCSIGTSTTTWNGSPTISTR
                     LSGGLYQSHNDCQATDIITYNCNGGAQSNTYYTDANNAWTAASQASSAQAQYGARGTY
                     NYYNGVHTRLSWNGSSGDMISYNNAFAGSNNACWGCTGNSTIYYAGNTSAATDDWNTL
                     DIMGHEFTHGVTQDEAGLIYNKESGALNESFSDIFGDMVESYNTGINDWLVGDDRGAI
                     RSFLNPNSYGDPDTYNGTNYKTTIGCTPSSANDQCGVHSNSSIQNRMFYLLSEGGSGT
                     NDLGVEFNVTGIGRFVARDIGYRALVLYLSGSNGFIDAREAWLRAALDLYGNCSNEII
                     QVGNAWRAVGVESQSAQYNKNICGNYPALGTYAQAIHQLSAANGCATNITASASTVYF
                     SATDRVVMYPGFHAISGSKFYAYIEPCAITLIKKAATDTRSDAEKGFVNKGSDHLQKA
                     STIDKIAIAPNPFNNEITLSFEMEKSATVKVELYTALGKLVTTLLQDNTINAGRHNFT
                     YSTGHITSGVYFIKIEIDGQATVKKIIKM"
     gene            complement(2578..4491)
                     /locus_tag="PK736_06995"
     CDS             complement(2578..4491)
                     /locus_tag="PK736_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRC33174.1"
                     /translation="MTLDSKIPKGPLAEKWNNAKFQYKLVNPANKRKYDIIIVGTGLA
                     GASAAASLAELGYNVKTFCFQDSPRRAHSIAAQGGINAAKNYRNDGDSVHRLFYDTIK
                     GGDYRAREANVHRLAEVSVNIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYAKG
                     QTGQQLLLGAYSAMNRQIAAGNIKSYTRHDVLDVVLVKGEARGIIARDMVTGEIERHA
                     AHAVLLCTGGYGNVFYLTTYARGSNATAIWRAHKRGAFFGNPCYTQIHPTCIPVSGDH
                     QSKLTLMSESLRNDGRVWVPKAQGDKRKPEDIPEGERDYYLERKYPSFGNLVPRDIAS
                     RAAKEACDEGRGVGTTGLAVYLDFADVIKRLGKKSVEEKYGNLFDMYKQITGENPYET
                     AMRIYPAVHYTMGGLWVDYNLMTTIPGLYALGEANFSDHGANRLGASALMQGLADGYF
                     VIPYTVGDYLADVERKPVSADTVEFDEAEKAVRERLNKLISIKGNKTVHEYHRELGLI
                     MWNHCGMARNEQGLKHGMEKIRTLRSDFWNNILIPGSGDELNQNLEKAGRVADLIELG
                     ELMMMDALNRNESCGGHFREEYQHEDGEARRDDENYAYAAAWQYMGDTQPQQLHKEIL
                     DYEFVHRSTRSYK"
BASE COUNT         1305 a          936 c          940 g         1391 t
ORIGIN      
        1 atattataaa aatgtaaaag tgctgcatgg tcatttcaag atacatcaac gtgccgatgg
       61 aatggtgcaa acggcaaatg gaaatatcat aaccgggttg aatttggacc ttaccactac
      121 tgtaaatgaa aatgaagccc tagcaaatgc gttaagtttt atgaatgcca aaaagtattt
      181 gtggcaaaac gaagtttcgg aaaacgaatt gaaacgtacc gaaaataatg tgaatgcaac
      241 ctactttcca aaaggcgaat tagtatttgc tcctgccaat tatgatgtta cttatcttgc
      301 ggctgattat agattgacgt ggcaatttac aatttacagt gatgatataa atgttgctgc
      361 aaaatgtgtt tttgtagatg ctttatctgg caaggttgtg taccattatg atatttctat
      421 gtcgtgcagc atcggcacta gcactacaac ttggaatggt tcgccaacta taagtactag
      481 acttagtgga ggcttatatc aatcgcataa cgactgtcaa gcaactgata ttattactta
      541 caattgtaat ggtggggcgc aaagcaatac ttattatacc gatgcaaata atgcgtggac
      601 tgccgcctca caggcatcat cggcacaggc gcaatatggt gcgcgtggca catacaatta
      661 ttataatggt gtgcatactc ggttgagttg gaatggctca tcgggcgata tgatttctta
      721 caacaatgcg tttgctggat cgaacaatgc ttgttggggc tgcaccggaa attctacaat
      781 ttattatgcc ggcaatacct cggcagctac cgatgattgg aatactttgg atataatggg
      841 acatgagttt acccacggtg taacacaaga tgaggctggc ttaatataca ataaagaatc
      901 gggcgcgtta aacgaatcgt ttagcgatat ttttggcgat atggtagaga gttataacac
      961 agggattaat gactggctgg tgggtgacga ccgtggtgcc atacgaagtt ttttgaatcc
     1021 caactcctat ggcgatcccg atacttataa cggcaccaat tacaaaacaa caattggttg
     1081 tacaccttcg agtgcaaatg atcaatgtgg ggtgcattcc aacagtagca ttcaaaatcg
     1141 tatgttctat ttattgtcag agggtggttc gggtaccaat gatttgggag tggaatttaa
     1201 cgtaaccgga ataggaagat ttgtagcgcg cgatattggc taccgtgctt tggttttata
     1261 cttgtcgggc tccaatggtt ttattgatgc acgtgaagcg tggctgcgtg ctgctttaga
     1321 tttatatggt aattgttcaa acgaaattat tcaagttggc aatgcttggc gtgctgtagg
     1381 agtagagtcg caatcagcac agtacaataa aaatatttgt ggcaattatc ctgcattggg
     1441 tacctatgcg caagccattc atcagctgag tgccgccaac ggctgcgcca caaatataac
     1501 tgccagcgct tccacagtat actttagcgc cacagatagg gttgtaatgt atccgggatt
     1561 tcacgccatt tcgggctcaa agttttatgc gtatattgaa ccttgtgcca tcacattgat
     1621 caaaaaagct gctacggata caaggagcga tgccgaaaaa ggttttgtca acaaaggttc
     1681 agaccatttg caaaaagcga gtacgataga taaaatagca attgcaccca atcctttcaa
     1741 caacgaaatt accttgtcgt ttgaaatgga aaaaagcgca accgtaaaag tggaattata
     1801 tactgccctt ggcaaacttg ttaccacttt actgcaagac aatactataa atgccgggcg
     1861 tcataacttt acatactcta ccggccatat tactagcgga gtatacttta ttaaaataga
     1921 aattgacgga caagcaacag tgaagaagat tataaaaatg taatcgtttc tttattttta
     1981 aacaagctgc tccattcatt tgaagcagct tgttttgttt tctatcatac gttaagtgcg
     2041 attcatattc aaaataatgg tttcaaaata aacacacata aattgtctcg ctgcggtttt
     2101 ttgtatttct aaaattggaa agaaaggcgt tacttttttt acccgaattt atcagattgg
     2161 tgtttttttg caccatataa attgcgttat tttttataat atttgtggtg cttttttaat
     2221 tataagtggc aagcaaaatc taaattctca tgatacgtat acagtaagcg ttgtttgtag
     2281 taaagaccaa aaaaattaca aataaaaaag agtatttagg tatctgacta caatcaattt
     2341 ttgtaaatgg ttttgactaa gactagtgca aaaaatcaat tactgttttc tatcaagttg
     2401 caactgccca tttttttttg gtaatagaaa cacttgtgta tacaatcaaa acaaaaagca
     2461 ttattttatc aaattatgat tatggctttg aatgtcgcaa ctcaaaaaag cctgtttgta
     2521 caccacaatc aaattgaagt tgtactaaaa atgatgcccc gatgctgtta aaaaaaacta
     2581 tttgtagctg cgcgtactgc ggtgtacaaa ttcgtaatca agtatttctt tgtgaagttg
     2641 ttgcggttgg gtatcgccca tatattgcca agcagctgcg tatgcgtaat tctcatcatc
     2701 gcgccttgct tcgccatctt cgtgttgata ctcttcgcgg aaatggccac cacaactttc
     2761 gttacggtta agggcgtcca tcatcatcag ttcgccaagt tctatgaggt cagcaacacg
     2821 gccagccttt tcaagatttt gatttagctc atcaccacta cccggtatca aaatattatt
     2881 ccaaaaatcg gaacgtaaag tgcgtatctt ttccattcca tgtttaaggc cttgttcgtt
     2941 gcgggccatg ccacaatgat tccacattat taatcccaac tcgcgatgat attcgtgcac
     3001 ggttttattt ccttttatag aaataagttt attcaatctt tcgcgcacag ctttttcagc
     3061 ttcgtcaaac tcaactgtat cggcactcac aggcttgcgt tcaacatcgg caagataatc
     3121 gcctactgtg tatggtatta caaaataacc atcggcaagg ccttgcataa gtgcgctggc
     3181 accaaggcga ttggcaccat ggtcgctaaa gttggcttcg cccaaagcgt acaaaccggg
     3241 tatggtagtc atcaaattat aatcgaccca caaaccgccc attgtgtaat gtactgcagg
     3301 atatattcgc atggcagttt cgtaaggatt ttcgccagtg atttgtttgt acatatcgaa
     3361 caggttgcca tacttttctt ctaccgattt tttgcccagg cgttttataa catctgcaaa
     3421 atcgagatat acggcaagcc ctgtagtgcc cacaccgcgg ccttcatcgc aagcttcctt
     3481 ggccgcacgc gaagcaatat cgcgtggtac taaatttcca aacgaaggat atttacgttc
     3541 cagataataa tcgcgctcgc cttcgggaat atcttcgggc ttgcgtttat cgccttgtgc
     3601 tttaggcacc catacacggc catcattacg aaggctttca ctcatgagtg taagtttact
     3661 ttggtgatcg cccgacactg gtatgcacgt tggatgtatt tgtgtgtaac atgggttgcc
     3721 aaaaaaagct ccacgtttat gtgcacgcca tattgctgtt gcattgctac cacgtgcata
     3781 agtggtaaga taaaacacat tgccatagcc accggtgcaa agcaatactg catgtgcggc
     3841 atgacgttct atttctcctg ttaccatatc gcgggcaata atgccacgtg cttcaccttt
     3901 taccaatacc acatcgagca catcatggcg tgtatatgat tttatatttc cggcagcaat
     3961 ttgcctgttc attgcactat atgcgcccag taaaagttgt tgccctgttt gtcctttggc
     4021 ataaaaagta cgtgatacct gcgcaccacc aaacgagcga ttgtcgagaa gcccgccata
     4081 ttcgcgcgca aaaggaacac cctgtgctac acattggtct ataatattta cgctcacttc
     4141 ggccaggcga tgcacattgg cttcacgggc gcgatagtca ccacctttta ttgtatcgta
     4201 aaataaacga tgcacactat cgccatcgtt gcgataattt tttgccgcat ttattccacc
     4261 ttgtgctgca atactatgtg ccctgcgtgg gctatcctga aagcaaaatg tttttacatt
     4321 gtaacccaac tcggccagcg atgctgctgc cgatgcaccg gccaatccgg taccaactat
     4381 gataatgtcg tacttgcgct tattagcggg atttaccaac ttgtattgaa actttgcgtt
     4441 attccatttt tcggccagtg gtcctttggg aatttttgaa tctaaagtca tctatcgtgc
     4501 tttgtttggt ttaaaaaaat gtctttgtta tcgagtaata attaagcgtt tatttttgaa
     4561 tcatcttacc ta
//
LOCUS       DAORQA010000140         4490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4794_length_4490_cov_19.499887, whole genome shotgun sequence.
ACCESSION   DAORQA010000140 DAORQA010000000
VERSION     DAORQA010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4794_length_4490_cov_19.499887"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2703)
                     /gene="infB"
                     /locus_tag="PK736_07000"
     CDS             complement(<1..2703)
                     /gene="infB"
                     /locus_tag="PK736_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRC33175.1"
                     /translation="MSEEKSIRISKALKDFNVAIDHVKEVLDKIGVEVDKGPNTKISE
                     EAYRALENAFASDKQAKAVADSQARDKHKRENITIDINTAKNTKPNEKEIDDVGEIDL
                     KKALDEIKAHGSKAPAPAAKPPVVEKIETPPPVIISEKAVEPEKSEKAEETGEPEVIK
                     AKAKKNDGPVVLGTIDLEGLKKPKKKTTKAKEGKEDSEEVVETQKKKSSVSENEKTLE
                     EITDEKPVTESPKSTEQTSETETETPTPEAPETDNVIRASAPKLADIKVLGTITLPTE
                     SRGKRGPAYSTSANSADNKKKRKRKDKKPVTSSTDSTFKGDAQGRNRPPASSGGPGTN
                     TGTNTGARPPYGARPPQTGARTPQTGNRPPAGAPFKPGQKRPLPAQQKTEFTTKEIQD
                     QMKAVQEKIAGQKGKSKSAGRRRDKRQAFQEARSLEDQDSIRKIKVTEFVSANDLAKL
                     MDVPVTQIISACMSLGYNVSINQRLDAEMISVVAAEFEFEVEFVSADVQEAISEQVDT
                     PEDLLPRAPIVTVMGHVDHGKTSLLDFVRNTNVLSGEAGGITQHIGAYEVTLESGKKI
                     CFLDTPGHEAFTAMRARGAKVTDVAIIVIAADDSVMPQTIEAINHAAAAGVPIVFALN
                     KMDKSDANPERIREQLAKMNYLVEDWGGKYQCQEISAKKGLNIDKLLEKVLLEAEMLE
                     LKANPNRNAKGTVIEATLDKGRGFVVTMLVQTGTLNVGDVVLAGCYTGKVKAMFDERN
                     NRLKSSGPSTPVMILGLSGAAQAGDQFNVMDDEREARNIANRRLQLQREQGIRSHKHI
                     TLDEIGRRLAIGTFKELNLIVKGDVDGSVEALSDSLLKLSTTEIQVNVIHKSVGQISE
                     SDVLLASASNAIILGFQVRPSSTARKLAEVEEIDIRLYSVIYDAI"
     gene            complement(2781..4031)
                     /gene="nusA"
                     /locus_tag="PK736_07005"
     CDS             complement(2781..4031)
                     /gene="nusA"
                     /locus_tag="PK736_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRC33176.1"
                     /translation="MDHKILIESFAEFKEFKNIDRATMMNILKDVFAGMIRKKHNSDE
                     NFDIIVNPDKGDLEIWWNREVVDDEFAEDSFDYDENKHISLTEAHKIEPDFELGETVT
                     VEVPLDAFGRRAVLNGRQNLKNRIMELEKEAIYQKYKDRVGDIIIGEVYQVWKKEILL
                     KDDEGNELILPKTEQIPSDFYKKGDNVRSVILRVEMQGGTPKIILSRTAPAFLERLFE
                     LEVPEVFDGLITIKKIVREPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRN
                     ENIDVINFTSNVNLLITRALSPARISEIKIDEETKRASVFMKPDQVSLAIGKGGHNIR
                     LAGKLTGYEIDVYRDSEDESEDVDLDEFSDEIDAWIIDEFKKVGFDTAKSVITLPIDE
                     LVQRTDLEEETIREVVRILKSEFE"
     gene            complement(4091..4453)
                     /locus_tag="PK736_07010"
     CDS             complement(4091..4453)
                     /locus_tag="PK736_07010"
                     /inference="COORDINATES: protein motif:HMM:NF036577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HRC33177.1"
                     /translation="MSSTSAAITIEECTALSRHLYAKLDALGLLETHELEVSSPGMDT
                     PLKVPQQYYRRINREIKVIDANGKEYTGRLKSANDKGFEMEAITIIKEGKVKKEQKND
                     VAFNYTDIKETKLIFNFK"
BASE COUNT         1070 a         1002 c          841 g         1577 t
ORIGIN      
        1 tatggcatcg taaattacag agtataaacg tatatcaatt tcttctacct cggcaagctt
       61 acgtgctgtg ctgcttgggc gtacctggaa tcctaatatg atggcattag atgccgatgc
      121 aagcaataca tccgattcgg aaatctgacc tacagatttg tgtattacgt tcacttgaat
      181 ttcggtggtg cttaatttca ataatgaatc tgataaggct tctaccgaac catcaacatc
      241 accttttact ataaggttga gttctttaaa tgttcctatt gcaagacgtc ttccaatttc
      301 gtcaagggta atatgtttat gcgaacgtat gccttgctcg cgttgtaatt gcaatctgcg
      361 gttggcaata ttgcgtgcct cacgctcatc gtccatcaca ttaaattggt cgccagcctg
      421 tgcagcacca ctcaaaccaa gtatcattac aggggttgaa ggtcccgatg atttgaggcg
      481 gttattacgc tcatcgaaca tggcttttac ttttccggta taacatcctg ccaacacaac
      541 atcgcccaca ttgagcgtgc ctgtttgtac aagcattgtt accacaaatc cacgaccttt
      601 atccaaggtt gcttctatta cagtgccttt agcatttcta ttggggttag cttttaattc
      661 aagcatttcg gcctcgagca ataccttttc gagtagctta tcaatgttca atcctttttt
      721 tgcagatatt tcctgacact gatatttgcc accccaatct tcgaccaggt aattcatctt
      781 tgccaattgt tcacgtatac gttcgggatt tgcatccgat ttatccattt tatttaatgc
      841 aaaaacaatg ggtactcctg cagcggcagc atgatttata gcttctatgg tttgtggcat
      901 tacgctatcg tctgctgcaa ttacaattat ggctacatcg gtaactttag caccacgggc
      961 acgcatggca gtaaatgctt cgtgaccggg agtatcgaga aaacatattt ttttgccgct
     1021 ttccagtgtt acttcgtaag cacctatatg ctgtgttatt ccgcctgcct caccagataa
     1081 gacattggta ttgcgaacaa aatcgagcaa cgatgtttta ccatgatcaa catggcccat
     1141 tacggttaca attggtgcac gtggcaataa atcttcgggc gtgtcaactt gttcggaaat
     1201 agcttcctgc acatcggccg acacaaattc cacttcaaat tcaaactcgg cagctaccac
     1261 cgaaatcatt tcagcatcga ggcgttggtt tatggaaaca ttataaccca agctcataca
     1321 agctgaaata atttgggtta caggtacatc catcaatttt gctaaatcgt tggcgcttac
     1381 aaattccgtt acttttattt tacgtatcga atcctgatct tctaagctac gggcttcctg
     1441 aaatgcctga cgcttatcgc gtctgcgccc tgccgattta gattttcctt tttggccggc
     1501 aattttttcc tgcacggctt tcatttggtc ttgtatttcc ttggtagtaa attccgtttt
     1561 ctgttgtgca ggcaacggac gtttttggcc gggcttaaat ggcgctccag caggtggacg
     1621 atttcccgtt tgcggtgtgc gtgctccggt ttgcggaggt cgtgcaccat atggagggcg
     1681 tgcaccggta tttgttccag tgtttgttcc aggtccacca gatgaagctg gtggtctatt
     1741 tctgccttgg gcatctcctt taaaagtaga atcggttgag gatgttacag gctttttgtc
     1801 tttacgttta cgcttctttt tattatcggc cgaatttgca cttgtagaat aagcagggcc
     1861 acgtttgcca cgcgattctg ttggcaaagt aatggtgcca agcactttga tatcggcaag
     1921 tttgggagcc gatgctctaa tcacattgtc tgtttcgggt gcttccggag ttggtgtttc
     1981 ggtttctgtt tccgaagttt gttccgttga tttgggggat tcagtaactg gtttttcgtc
     2041 agtgatttct tccaatgttt tttcattttc agaaacgctt gattttttct tttgcgtttc
     2101 aactacttcc tctgaatctt ccttaccctc tttggctttg gtagtttttt tcttcggctt
     2161 ttttagtcct tccaaatcga tagtgcccaa tactaccggc ccgtcattct ttttggcttt
     2221 tgcttttatt acctcgggct caccggtttc ctctgctttt tcgctctttt caggctcaac
     2281 tgccttttca gaaataataa caggaggagg ggtttctatt ttttcaacaa caggtggttt
     2341 tgcagctggg gcaggggctt tggaaccatg cgcctttatt tcgtccagtg ccttttttaa
     2401 atctatctca cctacatcat caatttcttt ttcgttgggc tttgtgttct tcgctgtatt
     2461 aatatcaata gtgatatttt cgcgtttgtg tttgtcacgc gcttgcgaat cggcaacagc
     2521 ctttgcctgc ttatcgcttg caaaggcatt ttccaaagca cgataagcct cttcggatat
     2581 tttggtattg ggtcctttat caacttccac acctatttta tccaaaacct ctttaacgtg
     2641 atcaatggcc acgttaaagt cctttagggc tttgcttatt cttatgcttt tttcttcgct
     2701 cattaacagt tattaaatta taaaacaaat atcgctttta aatgctcaca aactacaaaa
     2761 gtagtgtttg tgaacattgc ttattcaaat tccgatttta aaatacgcac aacttcgcgt
     2821 attgtttctt cttccaaatc tgtgcgttgt accaactcat ctattggtaa agtgattaca
     2881 ctttttgcag tgtcgaatcc aactttctta aattcgtcaa ttatccatgc atcaatttca
     2941 tcgctgaatt catctaagtc cacatcttcc gattcatctt cactatcgcg gtatacatca
     3001 atttcatatc cggtaagttt tccagccaaa cgaatattgt gacctccttt tccaatagca
     3061 agcgaaacct gatcaggctt cataaatacc gatgcacgtt tggtttcttc gtcaattttt
     3121 atttccgaaa tacgcgctgg gcttaatgca cgggtgataa gtaggttaac attgcttgta
     3181 aagtttatta catctatgtt ttcgttgcgc aattcacgca ctattccatg aatacgcgag
     3241 cctttcatac caacacaagc ccctacagga tcaatgcggt cgtcataaga ttctactgca
     3301 accttggcac gttcgcccgg ttcacgcact atttttttaa tggtgatgag tccgtcaaaa
     3361 acttcgggca cttcaagttc aaacaaccgc tcaaggaaag caggtgctgt gcgcgacaaa
     3421 attatttttg gtgtgcctcc ctgcatctca acacgtaaaa ttaccgaacg aacattatcg
     3481 ccttttttat aaaaatcaga gggtatttgt tctgttttag gaagtataag ttcattgccc
     3541 tcatcgtctt taagaagaat ttcttttttc caaacctgat acacttcacc gatgataata
     3601 tcgcctacac ggtctttata tttttgataa atagcctctt tctcaagctc cattattcga
     3661 ttcttgagat tttgacgacc attcaataca gcccgtcttc caaatgcatc aagcggaact
     3721 tctaccgtaa ctgtttcgcc caattcaaaa tcaggttcta ttttatgtgc ttcggtaagg
     3781 ctaatgtgtt tgttttcgtc atagtcgaaa ctatcttcgg caaactcatc gtccactact
     3841 tcgcgattcc accatatttc caaatcccct ttatcgggat ttacaataat gtcgaaattc
     3901 tcatccgaat tgtgcttttt acgaatcata ccagcaaata catctttgag gatgttcatc
     3961 atggtggcgc ggtcaatgtt tttaaactct ttgaattccg caaacgattc aattaaaatc
     4021 ttgtgatcca ttgtctcatt atttttattt tattaatgct tgtgtccact ccttattggt
     4081 ggctatttgt ttatttgaaa ttgaaaatca gttttgtttc ttttatatct gtgtaattaa
     4141 acgcaacatc atttttttgc tcctttttta ctttgccttc tttaataatg gtaattgctt
     4201 ccatttcgaa acctttatcg ttggccgatt ttagcctgcc tgtgtattct ttgccattgg
     4261 catctatcac ctttatttcg cgatttattc tgcgataata ctgttgaggt actttaagcg
     4321 gtgtatccat acctggtgac gatacttcta attcatgtgt ttcgagcaaa cccaatgcat
     4381 ctaatttcgc atacaaatgt cggcttaagg cggtgcattc ttcaatggta atagctgccg
     4441 aggtagaaga aattgatata cgtttatact ctgtaattta cgatgccata
//
LOCUS       DAORQA010000141         4483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4802_length_4483_cov_9.509259, whole genome shotgun sequence.
ACCESSION   DAORQA010000141 DAORQA010000000
VERSION     DAORQA010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4802_length_4483_cov_9.509259"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..3636
                     /locus_tag="PK736_07015"
     CDS             25..3636
                     /locus_tag="PK736_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRC33178.1"
                     /translation="MRKNYFILPLIMMMASSVIMKAQTQKLQFTTFEISTERQTSLSI
                     PANQTFDGQQFFIIQFTETPTIGFQNEIKRLGVQLLNYIPNKAYYASCAVGFDAALLK
                     PFKVHAIIPMDSRLKIHSKITSGNIPNYAFRGQNLMEVNIKLFNVNPFDKMINNISGA
                     NMEILKTSNNSNTLSMLVPVSQIIVLAQLPYVQFIEPAQPEPRGESYLSSTNQRSNVI
                     STDYLGGQNYNGSGINIGLGDNGFVGQHIDFKGRLDQSKNAGNQSNHATYVAGCITGT
                     GNLDPVEKSAAWGSNLYAYQFPTNIDSALSSFDNLGVSVTNNSIGENCNDGYTIYAQT
                     CDNQIIQRSALMHIFSAGEIQQFQINCGFLDYYGTITGGTKQAKNVITVGSVSYDDVY
                     ANNSSRGPATDGRIEPKVVATGENVISTLPNNTYNTQSGTSSAAAAVTGVYAQLSQAY
                     KELNAATEAPSALMKAAIMNGADDIGEVGPDFIHGFGRVNARRSLGMIKNNQYFTSNL
                     TQGDSIDFPITIPANVKAAKIMLYWNDVDALINVTPTIVNDLDLKVIDPVAFENLPLG
                     LNSNPNNFTIILAAIPKVDHLNNEEQVVILDPVAGTYTVRVNAFNIPAGAQEFTVVYD
                     WLFDDVTITHPVGGESFVPGETEVVRWDGFADNGIYGVYFSPDSGITWDTINQFIGGN
                     QRLINWPVPSVVTGKGLMRIEKAGATSSFVNAPFNIMPVPTGVTFNYQCLDSMQISWN
                     PLPEATVGYQVWKLGANYMDSLSFTTDTFLTIYNLNPYDTNWFAVNAYGANEAKSRRT
                     IAIQSIIGLVNCVIAVDAGVSNLSPVGGTIQSCLIAPLSDVKMTITNFGIDTIQTMDV
                     GYILNGGTPVVETINQPILPGATLNFTFTTQINISAPGAYVLQVYNVLASDGNVFNDT
                     IVSNIAVLNSPPIALPYSEDFETFTNCSIASDCEATVCPLINGMVNLQNTVADDADFR
                     VNQGGTPTPQTGPANDHTLNDPNGHYIYFEASNCFNKSAFVVLPCVDLNAVTSPVFSF
                     WYHMRGQSMGDLHLDIFSAGAWIDDIMPVLSGPQGNPQGEWLNAQIGLDNYIGQNVAF
                     RFRGFSGSQSSSDMAIDDIGVGSIIGIKDITQAASMFIQPNPATNNIKVTVANLVKGK
                     CVFEILDLQGRVINTLTTSVNQSLVSQDMDVSKLAKGIYNLRVVNENKTYNQKLVIQ"
     gene            3715..>4483
                     /locus_tag="PK736_07020"
     CDS             3715..>4483
                     /locus_tag="PK736_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33179.1"
                     /translation="MKKNFLLLCILSVSFFVVNAKELTLKLKSGNLKVSESALAIPTN
                     QVWKDYKYFVIQFNQTPSLVAQQQLKQSGLILMDYLPDYAYYAAVNSSFNVTSLVNAN
                     VHAFVAMSDRIKMHPSLINHDYPSHALRGNNSIELTLKLYNNIDAEEVKNFLELSNVQ
                     VIQANNDYKVASVIVSINDIGKIASLPFIQYVEPIGPVGEPENYYERGSHRTNAISTE
                     YAGGRKYNGAGVTVGIGDDGWVGPHIDYTGRLDITQAA"
BASE COUNT         1424 a          816 c          882 g         1361 t
ORIGIN      
        1 catttattta aatttataaa aactatgcga aaaaattatt tcattttgcc attaatcatg
       61 atgatggcaa gttctgtgat catgaaagca cagacgcaaa aacttcagtt tactacattc
      121 gaaatcagca ccgaaaggca gacttcacta tcgattccag ccaatcaaac ctttgacggg
      181 cagcaatttt ttatcattca gtttaccgaa actccaacta tcggttttca gaatgaaata
      241 aaaagattgg gtgtacaatt gttgaactac attcccaata aggcttatta tgcctcatgt
      301 gcagttggtt ttgatgctgc tttgcttaag ccattcaaag tacacgcaat tatcccaatg
      361 gattcaaggt tgaaaatcca ttctaagatt acttcgggaa atattccaaa ttatgctttc
      421 cgcggccaaa atttgatgga agtaaatatc aaattattca atgttaatcc atttgataaa
      481 atgattaata acatcagcgg agccaatatg gaaattttga aaacaagcaa taattcaaac
      541 acgctttcga tgttggttcc tgtgagccaa ataattgtat tagcacaatt gccatatgtt
      601 caatttattg agcctgctca acccgagccc cgtggcgaaa gctatttatc gagtaccaat
      661 cagcgtagca atgttatttc taccgattat cttggcggtc aaaattataa tggaagtggt
      721 attaatattg gcttaggtga taatggattt gttgggcaac acatagattt caaaggccgc
      781 ttggatcaat ctaagaatgc tggcaatcaa tctaatcatg ccacttatgt tgccggttgt
      841 ataacaggaa caggaaattt ggatccggtt gagaagtcag ctgcatgggg ctccaattta
      901 tacgcatatc aattccctac taatattgat agtgccctaa gttcattcga caacttagga
      961 gtgagcgtta caaacaattc aataggagaa aattgcaacg atggctatac catttatgcc
     1021 caaacctgcg ataatcaaat aattcagcgc tcagcattaa tgcacatttt tagtgcaggt
     1081 gaaatacagc aatttcaaat aaactgtggt ttcttagatt attatggtac aattaccggt
     1141 ggtaccaagc aggcaaaaaa cgtcatcact gttggttcag tttcatatga tgatgtatat
     1201 gcaaacaaca gcagccgtgg gcccgcaact gatggccgaa tagaacctaa agtagtagca
     1261 actggcgaaa atgtaatctc tactttacca aacaatactt ataacacgca gtcgggaact
     1321 tcatctgcag cggctgcagt tactggtgtt tatgcacaat tatcgcaagc atacaaagaa
     1381 cttaacgctg ccaccgaggc tccatcagct ttaatgaagg ccgctattat gaatggtgct
     1441 gatgatatag gtgaagttgg tcctgatttt attcatggct ttgggcgtgt aaatgcaaga
     1501 aggtcattgg gaatgataaa aaacaaccaa tattttacca gcaacttaac gcaaggcgac
     1561 tctatcgatt ttcctattac cattcctgca aatgtaaaag ctgcgaaaat aatgttgtat
     1621 tggaatgatg tagatgcttt gataaatgta acaccaacaa ttgtaaacga tttggattta
     1681 aaagtgattg accctgttgc tttcgaaaat ttgccattag gtcttaattc aaacccgaat
     1741 aattttacaa ttatccttgc agcaattcca aaggttgacc atttaaacaa cgaagaacaa
     1801 gtagtaatac ttgaccctgt agcaggtacg tatactgtaa gagttaatgc atttaacatt
     1861 ccggccggag cgcaagaatt tactgtagtt tatgattggt tgtttgatga cgttactatc
     1921 actcatcctg ttggcggaga atctttcgtg ccgggcgaga cagaagtagt gcgttgggat
     1981 ggttttgccg acaacggtat ttatggagtt tacttttcgc ctgatagcgg catcacctgg
     2041 gatacaatca atcagtttat tggtggaaat caaaggctta taaactggcc tgttccaagc
     2101 gtggttactg gcaagggttt aatgagaata gaaaaagcag gtgcaacatc gagcttcgtc
     2161 aatgcacctt tcaacattat gcctgtgccc actggtgtta cttttaatta tcaatgctta
     2221 gattcgatgc aaatatcatg gaatccattg cctgaagcaa cagtaggtta tcaggtttgg
     2281 aaactcggtg ctaattatat ggacagttta tcatttacta ccgacacctt ccttacaatt
     2341 tacaatttaa atccatatga cactaattgg tttgcagtta acgcttatgg cgcaaacgaa
     2401 gcaaaaagca gaaggacgat tgcaattcag agcatcatag gattggtaaa ttgtgttatt
     2461 gctgtggatg caggagtttc aaatctttcg cctgtaggtg gcactattca atcatgttta
     2521 atagcacctc tatccgatgt aaaaatgact atcaccaact ttggtattga caccattcaa
     2581 acaatggatg tgggctatat tttaaatggt ggaactccag tggtagaaac tataaatcaa
     2641 ccaatattgc cgggcgcaac attgaacttt acattcacta cacaaataaa tatatccgct
     2701 ccgggtgcct acgtcttgca ggtatataat gtgctcgctt cggatggtaa tgtatttaat
     2761 gatacaattg tttcaaacat tgcagtgctt aactcacctc ctattgcatt gccatacagc
     2821 gaagattttg aaacctttac taattgcagt attgcatcag attgcgaggc tactgtttgt
     2881 cctttaataa atggcatggt gaatttacaa aacactgttg cggacgatgc cgatttcaga
     2941 gtaaaccaag gcggaacacc tacaccacaa acaggccctg ctaatgacca cactttgaat
     3001 gaccctaatg gccactatat ttatttcgaa gcatctaatt gcttcaataa atctgcattt
     3061 gtagttttac cttgtgttga tttaaatgct gttacaagcc ctgtgttttc tttttggtat
     3121 catatgcgtg ggcagtctat gggcgatttg catttagata tatttagtgc cggtgcatgg
     3181 attgatgata ttatgccagt tttatcaggg ccacaaggta atcctcaagg ggaatggctc
     3241 aatgctcaaa taggtttgga taattatata ggccaaaatg ttgcttttcg tttccgcggt
     3301 ttttctgggt cacaatcatc gagcgatatg gcaattgatg atattggcgt aggtagcatt
     3361 ataggaatta aagatataac tcaagctgcc agcatgttta ttcagccaaa ccctgctacc
     3421 aacaatatca aagttacagt agccaactta gtgaaaggta aatgtgtttt cgaaatcctt
     3481 gatttacaag gcagggtaat aaacacgctt acaacaagcg ttaaccaatc attggtttct
     3541 caagatatgg atgtatcgaa attggcaaaa ggaatttata atcttagggt tgtgaatgag
     3601 aacaaaacat acaatcaaaa actagttatt caataataaa aaagtataaa gaagcgaggt
     3661 gtaaagcctc gcttttttgt ttcaaaaata taaaacttaa aaattcaaaa aaaaatgaaa
     3721 aaaaacttct tattgttatg tattttaagc gtttcttttt tcgtggtgaa tgccaaagaa
     3781 cttacgctta agctcaaatc gggaaattta aaagtaagtg aatctgcact tgctattcct
     3841 acaaatcagg tatggaaaga ttacaaatat tttgtaattc agtttaacca aacaccttcg
     3901 ttggttgctc aacaacaact taaacaatcc ggattgatat tgatggacta cttacctgat
     3961 tatgcttatt atgctgctgt aaactcttca ttcaatgtta catcgcttgt gaatgcaaat
     4021 gtacatgctt ttgttgcaat gagtgacagg ataaaaatgc atccatccct catcaaccac
     4081 gattatccga gtcatgcttt gcgcggtaac aatagtattg aattaacatt gaaattgtat
     4141 aataacattg atgcagaaga agtgaaaaat tttcttgaat taagcaatgt tcaagtgatt
     4201 caggctaaca acgactataa agttgcaagc gttattgttt cgataaatga tattggaaag
     4261 atagccagct tgccttttat ccaatatgtg gaaccgattg gaccagttgg tgagccagaa
     4321 aattattatg agcgtggaag tcatcgtaca aatgctattt cgaccgaata tgccggtggc
     4381 agaaaatata atggtgccgg agtgactgtt ggtataggtg atgatggttg ggttggtcca
     4441 catatagatt acacaggccg cctcgatatt acacaagcag ctt
//
LOCUS       DAORQA010000142         4456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4834_length_4456_cov_7.271984, whole genome shotgun sequence.
ACCESSION   DAORQA010000142 DAORQA010000000
VERSION     DAORQA010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4834_length_4456_cov_7.271984"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..>516
                     /locus_tag="PK736_07025"
     CDS             265..>516
                     /locus_tag="PK736_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC33180.1"
                     /translation="MLSIKNLKARVEEKEILQGLNLQVNAGEVHAIMGPNGSGKSTLA
                     SVLAGKEEYEVTAGEIIFNGENISEFSPEERAQKGMFLAF"
     assembly_gap    517..526
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <527..1028
                     /gene="sufC"
                     /locus_tag="PK736_07030"
     CDS             <527..1028
                     /gene="sufC"
                     /locus_tag="PK736_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRC33181.1"
                     /translation="YPVEIPGVSNATFLKEAVNAMRVANGKEKLDSKDFLKLMKSKMA
                     LVEMDKVMTQRSVNEGFSGGEKKRNEIFQMAMLEPSLCILDETDSGLDIDALRIVANG
                     VNSLRSAERSFVVITHYQRLLDYIVPDFVHVLYNGRIVKSGGKELALELEEKGYDWVK
                     EESMGV"
     gene            1263..2540
                     /gene="sufD"
                     /locus_tag="PK736_07035"
     CDS             1263..2540
                     /gene="sufD"
                     /locus_tag="PK736_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HRC33182.1"
                     /translation="MTTLINDKIKSAAVTQKFPSLRTTAFASIDKQGFPTTKNEEWKY
                     TSLDKIISASYRDVFAAQHNHIAQQNISTLPFINNGDVVIVLENGQFRNDLCDVKNLP
                     PGISISSLNENENNEIVKAHFNKYVAIDKHPLAALNTALMQDGVFIHIDKNALIEKNI
                     YVLHFSSSNGEAVSNYTRLLLLAESGCKANISCLTLSHSETTQSLNNIVTEIVVDENA
                     DVTFDFIQNDNLLSNQITNTYAYQKRDSKFCINTITFGNKLTRNNLALNLDAENCEAH
                     MYGLIAGNGEQLMDNHTEVYHAKPHCNSNQIYKSLMGGKSTGVFNGKIFVLLDAQKTN
                     AYQSSKNILMSNDAVMYTKPQLEIFADDVKCSHGATTGQMDEAALFYLRARGINKPEA
                     QALLNKAFASDVMEHISNQVLKEYCDALLQSKI"
     gene            complement(2696..>3290)
                     /locus_tag="PK736_07040"
     CDS             complement(2696..>3290)
                     /locus_tag="PK736_07040"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33183.1"
                     /translation="NIEFYVDGEQVAKASYEAGRKDAIVLSNLAKFVPAGKHLLEVRY
                     SGCKNPLPYSLAVNYHTTLPVSNKDCKLNIKSKLAVNNAKTGETVRYTVTVANTTNDG
                     LPSTMAIIAIPAGCTFQPWQLKEMTDKKVMDYYEIRGNKLMAYYRQMKPDEVKTLNFD
                     LKAEVPGAYETPASTAYLYYTNELKDWCSAEKLTIIK"
     assembly_gap    3291..3326
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3327..>4456)
                     /locus_tag="PK736_07045"
     CDS             complement(<3327..>4456)
                     /locus_tag="PK736_07045"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33184.1"
                     /translation="PIVSKVDIEPMQAKVIYYNYKTGYGNGEYDFAISATANGMSDAM
                     EQKIKVSPKGFPVQQSFSSNQMHDKFTTSLNDVVPGSVDVTLTAYPNIVSDLLKGVDA
                     ILREPYGCFEQTSMSSYPNVLVMEYLKTSGQEDPKIMAQAEKLIDKGYKMLTKYEASQ
                     RGYEWFGSNPGHEALTAYGLMQFNDMKSVYPNLDEKMIKRTADWLTARKDGKGGFLRD
                     GKALDSYGRASEDVTNAYITYALSAAQTPNLEKEIDYSFDKAEASGDAYMLALSANSL
                     FDHNRDRRGEKIVKELLKMQNENGSWTGKSHSITYSTGNALTVETTSIALMALLKSEN
                     VPFEQVNSAANYIVKARNGAGDFGNTQSTIMALKALTAYAKY"
BASE COUNT         1427 a          856 c          837 g         1290 t
ORIGIN      
        1 atacttctgg cttgcaagtt aaaatatata gagcaaaacg aatataaaag tatagacgga
       61 gaattggatg aaatatcaaa attgatttca ggccttctca attatcttaa atcatcagaa
      121 ctgaaaggaa ctaaatttaa aaaataacac agacaaaatg ataacaaata aaactaatca
      181 tattacagtc attttaaaac taaaaacgca aaactcagaa cccaaaaccc aaaactaaaa
      241 acacagaact aaaaacacaa aaaaatgtta tcaataaaaa accttaaagc cagagtagaa
      301 gaaaaggaaa ttttgcaggg gcttaactta caagtgaacg cgggcgaagt gcacgcaata
      361 atgggtccta atggaagtgg caaaagcaca ctggcatcgg tacttgctgg caaagaagaa
      421 tacgaagtta ccgcaggcga aataattttc aatggcgaaa acatttcaga attcagcccc
      481 gaagagcgcg cacaaaaagg aatgtttctc gcctttnnnn nnnnnngtat ccggtagaaa
      541 ttcccggagt tagcaatgcc actttcctca aggaagcagt aaatgctatg cgtgttgcca
      601 atggaaaaga aaaattggac tcgaaagatt ttttgaaact catgaaatcg aaaatggcac
      661 ttgttgaaat ggacaaggta atgacacaac gaagtgtgaa tgaaggattc agtggtggcg
      721 aaaaaaagcg taacgaaatt tttcaaatgg ccatgctcga gccctcgctt tgtatacttg
      781 acgaaaccga tagcggactc gatattgatg ccctgcgtat agttgcaaat ggtgtaaatt
      841 ctttgcgtag tgccgaacgt tcgtttgttg ttattacaca ctatcagcga ttgctggatt
      901 atatagttcc cgattttgtg catgtgcttt acaacggccg catagtaaaa agcggaggca
      961 aagaacttgc gttggagttg gaagaaaaag gatatgattg ggtgaaggaa gagagcatgg
     1021 gtgtttaaat tgttcaatgc ttttgaatgg ttagaaataa taaagtcaaa aaaaaatatt
     1081 agatatacga ttgtttttca atttgaactg aacaatttgc caaacagctt atacaaatca
     1141 gttgtgggaa ataaattgtt ggaaaatgaa tagaagaaat ttacacagtt tataaaattc
     1201 gaaaacagac caaaggacaa attggacgta aaagagttat taaaattaaa taagaaaaat
     1261 aagtgacaac actcataaac gataaaataa aatctgctgc ggtgacacaa aagtttccat
     1321 cgctgcgtac aactgcattt gcaagtatcg acaagcaagg atttcctacc accaaaaacg
     1381 aagagtggaa atataccagc ctcgataaaa ttataagtgc cagctaccgc gatgtttttg
     1441 ccgctcaaca taaccatatt gctcaacaaa atatttctac gttgccattt atcaataacg
     1501 gtgatgttgt tattgtttta gaaaacggtc aattccgaaa cgatttatgc gatgtgaaaa
     1561 atttgccgcc aggcatttct atttcatcac ttaacgaaaa cgaaaacaac gaaattgtaa
     1621 aagcccattt caataaatac gttgcaatag acaaacaccc attggcagca cttaacactg
     1681 cattgatgca agatggtgtt tttattcaca tcgataaaaa tgcattaatt gaaaaaaata
     1741 tttatgtgct ccatttttca tcaagcaatg gagaggcagt gtccaattac acacgactgt
     1801 tgcttttggc cgaaagcggc tgcaaggcca acatatcatg cctcactttg tcgcacagcg
     1861 aaaccacaca atcgttaaat aatatcgtaa ctgaaattgt agttgacgaa aatgccgatg
     1921 tcactttcga ttttatacaa aatgataatt tattaagcaa ccaaattaca aacacgtatg
     1981 cttatcaaaa acgcgattct aaattttgca tcaacaccat tacattcgga aataaactaa
     2041 cacgcaataa tttagcacta aacctcgatg ccgaaaattg cgaagcacac atgtatggat
     2101 taattgccgg caatggcgaa cagctgatgg acaatcatac cgaagtttac catgccaagc
     2161 cacattgcaa tagcaaccaa atttataaaa gcctgatggg cggcaaatcg acaggcgtgt
     2221 ttaacggaaa aatatttgtg ttgctcgatg cgcaaaaaac aaacgcctac caaagcagca
     2281 aaaatatatt aatgagcaac gatgcggtga tgtacacaaa accgcaatta gaaatttttg
     2341 ccgatgatgt aaagtgcagc cacggtgcca ctaccgggca aatggatgaa gcagcattgt
     2401 tctatttacg cgcacgcggc atcaacaagc ccgaagcaca agcactatta aacaaagcat
     2461 ttgccagcga tgtaatggaa cacatcagca accaagtctt gaaagaatat tgcgatgctt
     2521 tgttgcagag taagatttaa ttaaagctat tcgttttttt tatattgaag ttgtattccg
     2581 aattgcggtc gtaagaattc ctcaaaagta atcgcttcta tctgaacaat tattaacaca
     2641 acaaaagcga taaggtgaag aaccttatcg ctttacattt cagtcgttgc agctattatt
     2701 ttattatagt caacttctct gcactgcacc aatctttcaa ttcattagtg tagtataaat
     2761 aagctgtaga tgcaggtgtt tcgtaagcac cgggcacttc agctttgagg tcgaagttta
     2821 gtgtttttac ttcatcaggt ttcatttgtc ggtaatatgc catcagttta ttgccacgaa
     2881 tttcgtagta gtccataact tttttatcgg tcatttcttt caattgccag ggctgaaaag
     2941 tgcagcctgc aggtattgca ataatcgcca tagtacttgg caagccatcg ttggtggtat
     3001 tggcaacggt aactgtatac cttactgttt caccggtttt agcattattc actgccagtt
     3061 tcgattttat atttagctta caatctttat tgcttacagg taacgttgta tgataattaa
     3121 cagccaaaga gtatggcaaa ggatttttgc agccactgta acgtacttca agcaagtgtt
     3181 ttcctgctgg tacaaatttg gcaaggttgc tcaatacaat tgcatctttg cggccggctt
     3241 cgtatgatgc ttttgcaacc tgttcgccat caacataaaa ttcgatgttg nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnngtac ttagcatatg cggtaagtgc ctttaatgcc
     3361 atgattgtac tttgagtgtt gccaaaatcg cctgcaccgt tgcgtgcttt tacgatataa
     3421 tttgctgcac tattcacttg ttcgaatggc acgttttcgc ttttcaataa agccatcagt
     3481 gcaatggatg ttgtttctac cgtgagtgca ttacctgtac tataagtgat gctgtgcgat
     3541 tttcctgtcc aacttccgtt ttcgttttgc attttcaaaa gttcctttac tattttttcg
     3601 ccacgcctgt cgcggttatg atcgaataaa gaatttgccg acagtgccaa catataggca
     3661 tcgcccgatg cttcggcttt atcaaaagaa taatctattt ccttttcaag attaggagtt
     3721 tgtgctgccg acaacgcgta tgtgatgtat gcatttgtaa catcttcgct tgctcggcca
     3781 tagctatcga gtgccttgcc atcgcgcaaa aatcctcctt taccatcctt acgggcagta
     3841 agccagtcgg cagtgcgctt tatcattttc tcatcaaggt ttggataaac agatttcata
     3901 tcgttgaact gcataagtcc gtaagcggtt aatgcctcat gacccggatt agaaccaaac
     3961 cattcatatc cgcgttgcga tgcctcatat tttgtaagca ttttatatcc tttgtctatt
     4021 aacttttcag cttgcgccat tattttcgga tcctcttgtc ccgatgtttt taaatattcc
     4081 attaccaaca cattgggata gctactcata cttgtttgct cgaaacaacc ataaggttcg
     4141 cgcaaaattg catcaacacc ttttagcaaa tcactcacaa tgtttggata agctgttagg
     4201 gtaacatcaa ccgaacccgg tactacatca tttaacgatg tggtaaattt atcgtgcatc
     4261 tgattgctgc taaacgattg ttgaacagga aatccttttg gtgatacttt aattttttgc
     4321 tccatagcat cgctcatgcc attggcagtg gcacttattg caaaatcata ttcgccattt
     4381 ccataaccgg ttttataatt gtagtaaatt acttttgcct gcataggttc aatatctact
     4441 ttgctaacta taggtg
//
LOCUS       DAORQA010000143         4449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4846_length_4449_cov_33.076695, whole genome shotgun sequence.
ACCESSION   DAORQA010000143 DAORQA010000000
VERSION     DAORQA010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4846_length_4449_cov_33.076695"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PK736_07050"
     CDS             <1..294
                     /locus_tag="PK736_07050"
                     /inference="COORDINATES: protein motif:HMM:NF037412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33185.1"
                     /translation="VTKLNDSIYYSCWEDYLHSLLLHNYFFADTAYTLEGFYLESNTM
                     YAVVSQPFVTATQVTYLSQVKLFLEANGFFNNRNHDYFILRVVRFSNENITYS"
     gene            356..469
                     /locus_tag="PK736_07055"
     CDS             356..469
                     /locus_tag="PK736_07055"
                     /inference="COORDINATES: protein motif:HMM:NF037412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33186.1"
                     /translation="MLGIILEELHDENVLTENVVLRFIDSVFYLTDNFFEK"
     gene            543..698
                     /locus_tag="PK736_07060"
     CDS             543..698
                     /locus_tag="PK736_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33187.1"
                     /translation="MKIEDNGAGFNINELKHRGGLTNLQHRAGEMNATLKIESMIGQG
                     TFVELIL"
     gene            1060..2322
                     /locus_tag="PK736_07065"
     CDS             1060..2322
                     /locus_tag="PK736_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33188.1"
                     /translation="MKLKTIALTCIATVATMMATQAQTWNVGGNAVGAASKVGTTTNF
                     PMQMITNNATRMTVTSAGKVGIGTTAPTATFQVQKSTLADVLVKSTGNGAQVTIDRAG
                     NGYEAVTRYAQTGSTLWKTGLNVNSIGTAEYVIRNENIFADAMTIGSLTNNVGIGTAD
                     LSRAKLVVEGNVGKTTAIFGQGSTGISLVQNWPGIGFNSYYGTNVWKSMAAGYAAQMF
                     LDPSNGTLNYAFSNNAPGADQSLSQVVRMAITPDGRLILNSTNYTSTLAVKKVATAND
                     AASFQGTTLYSHFAFGTNEDTYIRGGLNSSNVIIADLNNNVGIGTANPAYKLDVCGTM
                     RAKEVRVATGWCDYVFADDYKLPSLKSVEEFIKENKHLPDVTPGAIIESEGLEVGKTS
                     AQMIKKIEELTLYVIELQKQVNELKSQK"
     gene            2350..3789
                     /locus_tag="PK736_07070"
     CDS             2350..3789
                     /locus_tag="PK736_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HRC33189.1"
                     /translation="MKKLNRRAQQFVFVLILFTIYCSQNIVTAQGSLDKPLQSIDKQN
                     DDVPIVPKTCGSYTIDKAALKQAIDFENSSSNKTQISSYTIRVYFHICRNDNGSNAAA
                     TLEQVKSEFNELIADFAPMNICFVSMGNHFIDNTTLNNNPTANFVSAFTLSGCLNIFY
                     HYNMPGVGGSAWAIPNTFCSIDRSNIGAAHTTSHEVGHCLGLLHTFEPVNGFEKINGT
                     NSSTAGDQITDTPADPYVYNGQGCFSTSGCSYNGNCTDPNGATNFNPPYTNTMAYWPV
                     TFGNFCVPNPVFTSGQFTRVNSFLSTNINLTPLWTPWQNLYYNGTSYTSGIHWDVATD
                     NIGNTGSLSITGSAIATLGANSIILGPGFIATPSSLGLVLIRPTACSAGNGIPKYNNN
                     LFDDQVEANEMNLYPNPASDVISINIPSLNAASTFNIYNSTMQLIKTLNFESGIENTI
                     STADLPPGIYFIDAQSDNQHWRQKFIINR"
BASE COUNT         1442 a          821 c          835 g         1351 t
ORIGIN      
        1 gtaacaaaac ttaacgactc aatttattat tcgtgttggg aagattattt acacagtttg
       61 cttttgcata attatttttt tgctgatact gcgtatacac tcgaaggttt ttatttagaa
      121 agtaacacta tgtatgcagt agtttcacaa ccttttgtaa cagcgacaca agtaacttat
      181 ttgagtcagg tgaaattatt tttagaagcg aacggctttt tcaataacag aaatcatgat
      241 tattttatac taagagttgt tagattttcg aatgaaaata taacttactc ttagtaatac
      301 cgaacaactt caatagtttg tttttccaaa acctaagttg tttaggtata atccagtgct
      361 cggaataatt ttggaggagt tgcatgacga aaatgtgtta acagaaaatg tagttttgcg
      421 atttatagat tcggtgtttt atttgactga taactttttt gaaaagtaaa attattgtat
      481 gtcagttttt caaacaaatt ttgcataatc agcaaataga ttttgatgac aaacatatcc
      541 acatgaaaat agaagacaac ggtgcaggct tcaacatcaa tgaattaaaa catcgcggag
      601 gcttgaccaa tttgcaacac cgggccgggg aaatgaatgc cacattgaaa atagaaagca
      661 tgatagggca ggggactttt gttgaattga ttttgtgatt gtacaaatgt ggattcattg
      721 atttattttt cttcgcaaaa aaagtagaac accgttgaaa ctgatttaaa aagatttaaa
      781 aaatccgcac tcatccgttg tatcagcgtg agcagtgttc catcaggcta gtatgtttag
      841 ttttgcgaaa tttctcatct caaattctta attcctaatt cttaatttct catccccaat
      901 ccccaatttc tcatccccaa ttcttaattt ctaatttcca aaagcaaggg taaaccaccc
      961 acatgtttat gggggttctt tattcatttg ttcactttat gtttgctgcc gttaattaac
     1021 acaaacaaaa aaatactaac aatttaaatt attaaaaaaa tgaaattaaa aacaattgca
     1081 ttaacatgca tcgcaacagt tgccacaatg atggcaacac aagcacaaac ctggaatgta
     1141 ggcggcaatg ccgtaggtgc tgcatccaaa gtgggtacta caacaaattt ccccatgcaa
     1201 atgattacca acaatgcaac acgcatgacg gtaacaagtg caggtaaagt tggtattggt
     1261 acaactgcac ccacagcaac atttcaagtg cagaaaagta cattagccga tgtacttgtg
     1321 aaatcgacag gcaatggtgc acaagttaca attgaccgcg caggtaatgg ttacgaggcg
     1381 gttacacgtt acgcacaaac cggcagcacc ttgtggaaaa cgggactgaa cgtaaattca
     1441 attggtactg ctgagtatgt tattagaaat gaaaatatat ttgctgatgc tatgacaatt
     1501 ggaagcctta ccaacaatgt aggtatcgga actgctgatt tatctagagc aaagttggtt
     1561 gttgaaggca atgttggaaa aacaacagca atttttggac aaggctctac agggatttcc
     1621 ttggttcaga actggccagg catcggattt aattcatact atggaactaa tgtttggaaa
     1681 tctatggctg ccggctatgc agcacaaatg tttcttgatc cttcaaacgg aacacttaat
     1741 tatgcttttt ccaataatgc acccggtgca gaccaatctc tgtctcaagt tgttagaatg
     1801 gccataacac ctgatggcag gttaatttta aattcaacta actacactag cactttagct
     1861 gtaaaaaagg tggcaactgc taacgatgct gcaagttttc agggaacaac tctttactct
     1921 cattttgctt tcggaacaaa tgaagatacc tatatcagag ggggtttgaa ttcttccaat
     1981 gtaatcatag ctgatttaaa caataatgtc ggaataggca cagcaaaccc tgcatacaaa
     2041 ttagatgtat gcggcaccat gcgtgctaag gaagttcgtg ttgcaacagg ttggtgcgat
     2101 tatgtttttg ctgatgatta taaacttcca tcattaaaat cggtagaaga atttattaaa
     2161 gaaaacaaac acttgcctga tgttactccg ggtgcaataa tagaaagcga aggtttagaa
     2221 gtaggtaaaa catctgcaca gatgataaag aaaatagaag aacttacgct gtatgtgata
     2281 gagttgcaaa agcaggtgaa tgagttgaaa tcacagaagt aaattgctaa ccatttaaaa
     2341 ttttaaaaaa tgaaaaaatt aaataggcgt gcacaacaat tcgtttttgt gttgatattg
     2401 tttacaattt attgttcaca aaacattgtc actgcgcaag gaagcctgga taaaccatta
     2461 caaagtattg acaaacaaaa tgatgatgtt ccaatagtac caaaaacatg tggatcatat
     2521 accattgata aagccgcact aaagcaagcg attgattttg aaaattcaag ttcaaacaaa
     2581 acacaaatat catcatatac cattcgtgtt tattttcata tttgccgaaa tgataatggc
     2641 tccaatgctg cggcaacgtt ggaacaagta aaatctgaat ttaatgaatt aattgcagat
     2701 tttgctccca tgaatatttg ttttgtaagt atgggcaatc attttattga taacactact
     2761 ttaaataata accctacagc taactttgtt agtgctttta ctttaagcgg atgcctcaat
     2821 attttttacc attataatat gccgggagtt ggaggtagtg catgggcaat accaaatact
     2881 ttttgctcca tagacaggag taatattggc gcagcgcaca caacatcgca tgaagtagga
     2941 cattgcttag ggttactaca tacatttgag ccggttaatg gattcgaaaa aattaatgga
     3001 acaaactcat caactgctgg cgatcaaatt actgatacac ctgccgatcc atatgtgtat
     3061 aatgggcagg gttgttttag cacttcgggc tgttcttata atggaaactg tactgatcct
     3121 aatggggcaa ctaatttcaa tccaccttat accaatacaa tggcttattg gcctgttaca
     3181 tttggtaatt tttgtgttcc caatcctgta tttacttcgg ggcagtttac tcgtgtaaat
     3241 agttttctca gcaccaatat taatttaaca ccactgtgga cgccatggca aaatctatat
     3301 tataatggta cctcttacac ttcaggaatt cattgggatg ttgctaccga taatattggt
     3361 aatacgggaa gtttatcaat taccggatct gcaattgcta cacttggagc aaactcaatc
     3421 attttgggac ctggttttat tgcaacacct tcttcgcttg gacttgtgtt gattcgcccc
     3481 acagcatgca gcgcaggtaa tggtatacca aaatacaaca acaatttatt tgatgatcaa
     3541 gtcgaggcaa acgaaatgaa tctgtatcca aatcctgcat ctgatgttat tagtataaat
     3601 attccgtctc taaatgctgc ttcaactttc aatatttaca acagcaccat gcagttgatt
     3661 aaaactttga attttgaaag cggaattgaa aacacaattt caactgctga tttaccgccc
     3721 ggcatttatt ttatcgatgc acaatctgac aaccaacatt ggcggcaaaa atttattatc
     3781 aaccgttagg caatttttga aaagagcgac acagttttgt tgtcgctctt tttttctggc
     3841 ctcgcctcat gtccccaaca accgtggtca aattttgact ccgtaaaaac tgactcacat
     3901 ttttttgatt tctgtcctcg gttggcgtcc ccgccaaccg catgttgtag acgaaatatt
     3961 ttatctcctc aaaaaaggag ggttacattt tttgttcagt ccacaatcta acaaagagaa
     4021 ataaatggct attccacagc ggttggcggg gacgccaacc gaggacaagt ctttggtgat
     4081 agtgaagtaa aaataaattt attcgtcacc gtgaaaacaa aaagtattga gcattttagt
     4141 gcgccacatt tttctgtact cggttggcgt ccccgccaac cgcatgttgt agacgaaata
     4201 ttttatctcc tcaaaaaaga gggttacatt tttgttcagt ccacaatcta acaaacagaa
     4261 ataaattggt attccacagc ggttggcggg gacgccaact gaggacgcca attgatgaga
     4321 ccaaccgagg acaaaagaga atctcctaaa agaatcagac acacacttag aaagttttgt
     4381 gcaagaaatt tatatttact tttctgtcct cggttggcgt ccccgccaac cgcatgttgt
     4441 agacgaaat
//
LOCUS       DAORQA010000144         4414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4887_length_4414_cov_8.098646, whole genome shotgun sequence.
ACCESSION   DAORQA010000144 DAORQA010000000
VERSION     DAORQA010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4887_length_4414_cov_8.098646"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..2003
                     /locus_tag="PK736_07075"
     CDS             231..2003
                     /locus_tag="PK736_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HRC33190.1"
                     /translation="MQKFTLLKSAVVLTIIGGLTYAGLRNTRIEASSHREAPLICNDP
                     VADNTDLYAFRSPDKPGFITVMANYVPFELPMGGPNYSSFGENVRYEIHIKNNAATTS
                     DDITYRFTFTKMNEDPSTFFNIRLGAQNLKTTFKCEKKKGAGAFTTIVSNGTVPPPNI
                     GPRSIDTPGLGLGKSYAQIMQDAITNASGNGGEIIYCGPADDPFFVDLGGIFDLGQTR
                     QNFTGVLRARDGVGGFNVHTIAMQIPIATLQKDNKPVTMRKNILDPDFVIGVWASASR
                     QEVKTIRGMGQKPLYTGNWVQVSRLGMPLTNEAVIPIGGKDKWNARTPYNEDPAMEAS
                     FCNPELGLYMDNSLFGPAVPSLADLRIQRASPTILGNVDFGNTHDGLFVLYGHPATTG
                     TALDTAIFGKYLLRAGQPRSADLLPIFYTGVPNLAPYQLAVKKTAGNPLSAGKPFINN
                     FLPTFGDMLRLNMAVPPTQRNDPKFSSLGIIAAAVEGLTNPIYNNKAIQDIPNMDGFP
                     NGRRLEDDVTRIELQAVSGVALAACGLFYDDYTIGQSPLTPGLLKVLGYTTGLEKNDA
                     PFKTSFPYVAAPWSGYEYHFANTY"
     gene            2060..4153
                     /locus_tag="PK736_07080"
     CDS             2060..4153
                     /locus_tag="PK736_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HRC33191.1"
                     /translation="MFNTKSIIKTTLVLAVVAGCFAFGIRQSKLTASSHREAPLIADD
                     PIADNTDLYAFRSPDDSTTVTIIAAYNPIQLAQGGPNYYQFGEDVRYEIHIKNSTVAP
                     TTDDITYRFTFSKTNEDPSTFFNIRLGQQNLKTTYKMERWVRKVGGGYSTWITNGAVP
                     PANIGPRSINDPVLGLGKTYQQLVGAAITTSTSPEKEKVYCGPADDPFFVDLGGIFDL
                     GQTRAGGTGINAPEDGIACRNVNVIAIKVPIKFLQKDGKNAWNAQNILDPNFVVGVWA
                     SASRPKISTLNTDGTKPTTSGYYVQVSRLGMPLTNEAVIPLGSKDKWNSLTPYKEDPA
                     MEAYFCNPELGLYMDDSLFGGAVPGLAALRIQRASPTILGNVDFGNNNDGLHVLYGHP
                     ATTGTALDTAIFGKYLLRAGQPRSVDLLPIFYTGVPNLAPYQLATGKTAGNPLSAGKP
                     FINNFLPTFGDMLRLNMAVPVTPRNHPKFSSLGIIAAAVEGLTNPVYANTNMEWIPNM
                     DGFPNGRRLEDDVTRIELQAVSGAALAAVGLWYDDYTIGQSPLTPGLVNVLGYTTGIE
                     SNDLPFKKGFPYVANPWSGYGKCGGPKAATNGSNRIGMNVGVPTVVLMQNYPNPCKDF
                     TKFTYHVSADSYINISIYNTTGELVTVLVNEGKATGDHEVVWNLQGKIAPGVYYASIT
                     SNNTIGQTLKVNVTK"
BASE COUNT         1310 a          922 c          928 g         1254 t
ORIGIN      
        1 gttatctcct tgagatgcga acactttttg taattctttt attcgtgatt aaattttatt
       61 tcaaattttc aaatattttt ttctctactg tattgccttt aaacaaaggg attcagcaac
      121 attctatttt tcttggttac actttttata taacaattat ttaaaaaata aatccaagca
      181 caaaacttta gcgtaggtat cgcatattaa catttaaaaa agttttaaat atgcaaaagt
      241 tcacattact aaaaagtgca gttgtactaa ccattatagg tggtcttacc tatgccggtt
      301 tacgcaacac acgcatcgag gcatcatcgc atcgcgaggc gcctctcatc tgcaacgatc
      361 cggtggcgga caataccgat ttgtacgcct tccgcagtcc cgacaagccg ggcttcatca
      421 ctgttatggc caattatgtg ccattcgaat tacctatggg aggaccaaac tattcatcgt
      481 ttggcgaaaa tgtacgatat gaaattcata tcaagaacaa cgctgctact accagcgatg
      541 acatcactta ccgttttacg tttacaaaaa tgaacgagga tccaagtacg ttctttaata
      601 ttcgactggg agcacaaaat ttaaaaacca cttttaagtg tgagaagaaa aaaggtgcag
      661 gtgcgtttac aactatagtt agtaatggta cagtaccgcc accaaatata ggaccacgtt
      721 ctatagacac gcccggactt ggacttggta aatcgtatgc acaaattatg caggatgcca
      781 ttacaaatgc aagtggcaac ggtggcgaaa ttatttattg tggcccggcc gatgatcctt
      841 tctttgttga ccttggtggt atattcgatt taggacaaac acgccagaac tttacaggtg
      901 ttttacgtgc acgcgatggt gtgggtggat ttaatgtgca tactatagct atgcaaattc
      961 ctattgcaac attgcaaaaa gataacaagc ccgtaacaat gcgtaagaat attcttgacc
     1021 ctgatttcgt aattggagtg tgggcatcgg ccagccgcca ggaagtaaaa acaatcagag
     1081 gcatgggaca aaaacctttg tacacaggta actgggtgca agtgtcacgc ttaggaatgc
     1141 cacttacaaa cgaggcagtt attcctattg gtggaaaaga caaatggaat gcacgcacac
     1201 catataacga agatcctgca atggaagcat ccttctgtaa tcctgaactt gggttgtata
     1261 tggacaatag tttgtttggg ccagctgttc catcacttgc tgatttgcgc atacaacgtg
     1321 catcaccaac aattttggga aatgtggatt ttggaaatac acatgatgga ttatttgtgc
     1381 tttacggtca tcctgcaact acaggcactg cattggacac tgctattttt ggtaaatatt
     1441 tattgcgtgc aggtcaacct cgttcagccg atttgttacc aatattttat accggtgttc
     1501 caaaccttgc gccttatcaa ttagcagtga aaaaaacagc cggcaatcca ttgtcagctg
     1561 gtaagccgtt tataaataat ttcttaccta cgtttggtga tatgctacgc ctcaatatgg
     1621 ctgtgcctcc tactcaacgt aacgatccta aattctcttc gcttggaatt attgctgctg
     1681 ctgttgaggg attaacaaat cctatttata acaataaagc aatacaggat attccaaaca
     1741 tggatggctt tccaaatggc cgcagattgg aagatgatgt tacacgtatc gaattgcaag
     1801 cagtaagtgg tgtggcattg gctgcatgcg gattgtttta cgatgattac actattggtc
     1861 aaagtccgct aacaccgggg ttgttaaaag tattagggta cacaacaggc ctcgaaaaaa
     1921 atgatgcgcc tttcaaaaca tcgttcccat atgttgcagc accttggagt ggttacgagt
     1981 atcattttgc caatacttat taatatttta caatcactaa ctataactat atacgttaaa
     2041 aataaaaaaa cacaaaatta tgtttaatac aaaatcaata ataaaaacca ctttggtatt
     2101 ggctgtagta gcgggatgct ttgcattcgg aatacgccaa agtaaattaa cagcgagtag
     2161 ccatcgcgag gcaccattaa ttgccgatga tcctattgcc gataatacgg acctgtatgc
     2221 gtttcgcagt cccgatgaca gtactactgt aaccatcatt gcagcataca atccaataca
     2281 actggcacag ggcggaccaa actattatca gtttggtgaa gatgtacgtt acgaaattca
     2341 catcaagaac tcgaccgtgg cacccacaac tgatgacata acatatcgtt ttacattttc
     2401 aaaaacgaac gaagatcctt caacattttt taacattcgt ttaggtcagc aaaatttaaa
     2461 aacaacttac aaaatggagc gttgggtgcg taaagttggc ggaggttatt ccacatggat
     2521 tacaaatggt gctgtgccac cggctaatat agggccacgt tctataaacg atccggtatt
     2581 gggtttaggc aaaacgtatc agcaattagt aggtgcggct atcacaactt caaccagtcc
     2641 cgagaaagaa aaagtttatt gtggtcctgc tgacgatccg ttttttgttg acttaggtgg
     2701 aatattcgac cttggacaaa cacgtgccgg tggcacaggc atcaacgctc ccgaagatgg
     2761 aattgcttgc cgcaatgtaa acgtgattgc gataaaagtg cctatcaaat ttttacaaaa
     2821 agatggtaag aatgcatgga acgcacaaaa tattttggac ccgaattttg ttgttggtgt
     2881 ttgggcatcg gcaagtcgtc ctaaaatttc taccttgaat accgatggaa ctaagcctac
     2941 tacttcagga tattatgttc aggtatcgcg cttaggtatg ccacttacaa acgaggcagt
     3001 tattccattg ggcagcaaag acaaatggaa ttcccttacg ccatacaaag aagatcctgc
     3061 aatggaagct tacttctgca atcctgaatt gggattgtac atggacgata gtttgtttgg
     3121 tggtgcagtt cccggattag ccgcactgcg catacagcgt gcttcgccaa ctattttggg
     3181 caatgtagat tttggcaata ataacgatgg tttacatgta ttgtacggcc atcctgcaac
     3241 tacaggtact gcattggata ctgccatttt tggaaaatat ttgttgcgtg ccggtcaacc
     3301 tcgttcggta gatttgttgc caatatttta taccggtgtt cctaatcttg caccttatca
     3361 gcttgcaaca ggtaaaactg cgggcaatcc tttatcggcc ggtaagcctt tcatcaataa
     3421 cttcttacct acgtttggcg atatgttgcg ccttaatatg gccgtgcctg ttacgccacg
     3481 caatcatcct aagttttctt cactgggcat aatagccgct gctgtagagg gattaactaa
     3541 tcctgtatac gctaatacca atatggaatg gataccgaat atggatggat ttccaaatgg
     3601 ccgcagactt gaagatgatg ttacccgcat agaattgcag gcagtaagtg gtgccgcgct
     3661 agccgcagta ggtttgtggt acgatgatta tacaatagga caaagcccgc ttacaccagg
     3721 cctggtgaat gtattgggat atactaccgg tatcgaaagc aacgacttac catttaagaa
     3781 aggtttccca tatgtagcca atccatggag cggatacgga aaatgtggtg gccctaaagc
     3841 tgcaacaaat ggcagtaacc gtattggtat gaacgttgga gtgccgactg tggtgttgat
     3901 gcaaaactat cctaaccctt gtaaggactt tacaaagttt acctatcatg tttcggccga
     3961 ttcgtatatt aatatcagta tatacaatac cacaggcgaa cttgtaacgg ttcttgtaaa
     4021 cgaaggaaag gcaactggcg atcatgaagt agtatggaac ttacagggca aaattgctcc
     4081 tggagtttac tatgcttcta tcacttcaaa caataccata ggacaaacac taaaggtgaa
     4141 tgtaacgaag taattttttt aatgctgaaa tatttaaata caatcccatc ttcaatttag
     4201 tttgtatccc gccagggcaa tgcaaaaata aaaatgagtg aaataagggg tcattcatgc
     4261 aaccggttgg taaggaatta ccagccggtt tttttgtttg cgccagtcca catgcttaag
     4321 cccacccgcg tcagccaact cgcgtcagcc catccgcgtc agcccatctg cgtcagcaaa
     4381 cccgcgccag gccacccgcg cacgccgctg ttcg
//
LOCUS       DAORQA010000145         4383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4929_length_4383_cov_8.201248, whole genome shotgun sequence.
ACCESSION   DAORQA010000145 DAORQA010000000
VERSION     DAORQA010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4929_length_4383_cov_8.201248"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4181)
                     /locus_tag="PK736_07085"
     CDS             complement(<1..4181)
                     /locus_tag="PK736_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33192.1"
                     /translation="MLWILQSQHWLNNQGTINFNYTTNWDVSSGASVNTGVINIAAGV
                     TVTVKTGVINLPNTGAINFGNYLSKLNFTSATVNWSGPIVTGLGTMYFESSIANMDVA
                     TLPAIYMTFYGSTLASTFLPTTSTKKIVFNYNNTITAAIFNTDTFEIQNNYNVMSGAL
                     TTSNNAVLLFSGAVGYSSFATTAGFTNNGKIHFLSNGCNGTNLQCNVGTIINASTGLI
                     QSDPGAGCYGYNQMQGSIINQGTINVNYDLHWLLNAQTGENTGTIIISKTKQLYVHGG
                     TFNYNGGSTIINGTGTPPTTGNIYYYQTAAHMNVTEMPDAFTFFDYSSLNSNQLFMLN
                     NAQLQFNYGNTINANIQNRDTIIVSHSANIYAGIFFTNANSYLLLNGQNSSSVISFNY
                     GWTNNGLIRFTSNGCNATNLQMNGAAILTNPSGGVILSDLPGAGCYGYHVLTGAMNNE
                     GTLQLNYDLHWLLSTQTGTNTGSIIINRTKQLYIANGIFNYNGGTTTLTGTGVAPTTG
                     NVYYYQATANINVTEMPDVFTFFDYSILNSNQNPIINNARLQFNYGNTINAIMENRDS
                     VIISHSANTYAGNFTTNANSYLWFNGQNSSSVIIFNNDVTNNGLIEFTSNGCNYTQFN
                     MASGKTLTNANIGIVKSNLPGAGCYGYHRMDGRFINNGLLSLFYPLYNNGNSFINSAA
                     ATINGNSMLLLENTNLHNAGTISPGGTTAGLSIDATIKNTNTAIWNIQIGCITAITQH
                     DQIINNYSTRTDSINGTLNISLINGFVPGLGDSVIIETHNNRVGSFSTINGASIPGGL
                     VWNIFYRPNSIVLRAGNPPNLNIAATSGANGNISPSGVVSVVYNATQVFNFNPNSGYE
                     VDSVFVDGIYVDSTVTYTFANVQVDHTIHVTYQVITFPITATSGANGTVTPSGVTNVN
                     QFASQTYTITPGNCYMIDSVIVDGNYIGNPGTYTFNNVNGPHTIYASFRTVPIIVADG
                     PLSFCIGTVRNLDAGAGYANYIWSNGATSQIAQVSTSGTYTCTVTGGGGCTSSASVTV
                     VVNPLPVVSINPFYPADNNHVCLNGNNINGLIGSSYGMPVGGSYSGTGVFCVFFQCYW
                     VPSMAGLGTHTVTYSYTDSNGCSNTASMNVVVDTPATVNAGPSSMILCNTLVANLSGT
                     IGGTANSATWSTNGTGTFGDSTALITTYTFSTDDSLAGVVTLFLNSDNPSGPCGNIAD
                     NITIYYNPNAIADAGPAEACVNSTFNVSGTISGFGANPLWTSTGTGNFANPNAYATTY
                     TFSVADSIAGFVNIILTPIDPNGICTFTADTMKVWLNPAYSVNAGNDIVLCGINDTIA
                     TLNGSFSNAAQVTWSSSGGGTFSNIHDPNATYTLSAADRSNGYIFLSLVSDDPAGPCS
                     AVGDNIIIKYKYANV"
     gene            complement(4148..>4383)
                     /locus_tag="PK736_07090"
     CDS             complement(4148..>4383)
                     /locus_tag="PK736_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33193.1"
                     /translation="PPPPSTPHSDLHFPPQAAAPLTFATGWTNTGKILMSSPGCYLAT
                     LAITTGVLINPPTGIITINPNPACYGYYNLNIG"
BASE COUNT         1273 a          852 c          914 g         1344 t
ORIGIN      
        1 tcgacattgg catatttata ctttataata atgttatcgc ccacagcgga acaagggcct
       61 gcagggtcgt cacttaccag agataaaaat atatatccgt tacttctgtc tgcagctgat
      121 aaagtgtatg tggcattagg gtcatgaata ttactaaaag ttcctccacc cgatgatgac
      181 catgtgactt gcgcagcatt agaaaaagaa ccattcaaag tggcgatagt atcgttaata
      241 ccacacagta caatatcatt tcctgcatta acggaataag ccggattgag ccacactttc
      301 attgtgtcgg ctgtgaaagt gcaaatgccg tttgggtcga ttggcgtaag aataatgttt
      361 acaaatccgg ctatactatc agcaacagaa aaagtatagg tggtagcata tgcatttgga
      421 tttgcaaagt tgccggtacc ggttgaagtc cacaatgggt tggcaccaaa acccgaaatt
      481 gtacccgaca cattgaatgt gctattcaca caggcttcgg cagggccggc atcggcaatg
      541 gcatttgggt tataataaat ggtaatgtta tcggctatat taccacatgg gcctgacgga
      601 ttatcgctat tcaaaaaaag agttaccact cctgcaagag aatcgtcagt cgaaaaagtg
      661 taggtggtaa tcaatgctgt agaatcgcca aaagttccag tgccgtttgt tgaccatgtt
      721 gcactatttg ccgtgcctcc aattgtaccg ctcaaatttg caacaagtgt attgcacaat
      781 atcatggatg atggtccggc attaaccgtt gccggagtat cgaccaccac gttcatagaa
      841 gctgtattgc tgcatccatt cgaatcggta taagaatagg taaccgtatg agtgcccaag
      901 ccggccatgg aaggcaccca atagcattga aagaaaacac aaaatacgcc agttccgctg
      961 taactgccac ctacgggcat tccataactg ctgccaatca atccgtttat attgtttccg
     1021 tttaagcaaa catggttgtt atcggcagga taaaatggat tgatgctgac aacaggtaaa
     1081 ggatttacaa ctacagttac cgatgcagaa cttgtgcacc caccacctcc ggtaaccgta
     1141 catgtatacg tacccgaagt gctcacttgt gcaatttgcg aagtagcgcc attgctccaa
     1201 atataattgg cataaccggc acctgcatct aaattgcgta ctgtgcctat gcaaaaagat
     1261 aagggtccat cggcaacaat tattggcact gttctaaagg atgcataaat agtatgtggg
     1321 ccatttacat tgttaaatgt atatgtgcca ggatttccaa tataattacc atctacaatt
     1381 acactgtcaa tcatatagca attgcccgga gtgatggtgt aagtctgact tgcaaactga
     1441 ttaacattgg taacccccga tggtgtaaca gtgccattag cccccgatgt tgcagtgata
     1501 ggaaaggtaa tgacttgata tgtaacatga atagtatgat ctacctgcac atttgcaaaa
     1561 gtatatgtga cagtgctatc gacataaata ccatcaacaa aaacgctatc gacttcgtat
     1621 cccgaattgg gattaaaatt aaaaacctgt gttgcattgt atacaaccga tacaacgcct
     1681 gatggcgaaa tatttccatt ggcacccgaa gtagctgcta tatttaaatt gggtggattg
     1741 ccggcacgca atacaatact attggggcga taaaaaatat tccataccaa tccccctgga
     1801 atgctggcac catttattgt agaaaatgat cccactctgt tgttgtgcgt ttctattatt
     1861 acgctgtcgc caaggccggg tacaaatcca tttatcaagc ttatatttaa tgtgccattt
     1921 atcgaatcgg tgcgcgtgct gtagttgttt attatttggt catgctgcgt aatagcagta
     1981 atgcatccaa tttgaatatt ccaaattgca gtgtttgtgt ttttgattgt agcatctatc
     2041 gataacccgg ccgttgtacc tcccggtgaa atggtgcctg cattgtgcag gtttgtgttt
     2101 tcaagcaaca gcattgagtt gccattgata gttgctgcag cactatttat aaatgaattg
     2161 ccattattat acaaaggata aaataacgat agcaaaccat tattaataaa tcggccatcc
     2221 atgcggtgat agccatagca tcctgcaccg ggcaagttgc tttttactat tccaatattt
     2281 gcattggtca gcgttttgcc gctggccata ttgaactgcg tataattgca accattgctg
     2341 gtaaattcta taagtccatt atttgtaaca tcattattga aaataataac agaactgctg
     2401 ttctgcccat tgaaccacaa ataggaattt gcattagtgg taaaatttcc agcataagta
     2461 ttggccgagt gagatattat aacactatct ctattctcca taattgcatt gatggtattg
     2521 ccataattaa attgcaatcg tgcattattg ataatggggt tttggtttga gttcaatata
     2581 ctataatcga aaaatgtaaa tacatcgggc atttcggtta cattaatatt tgcagttgcc
     2641 tggtagtaat aaacatttcc tgttgttggc gcaacaccag tgcctgtcaa tgttgtggta
     2701 ccaccattgt aattaaatat gccgttagca atatataatt gctttgttct gttgataata
     2761 atactgcctg tgtttgtacc ggtttgtgtg cttagtaacc aatgcaaatc ataattcaat
     2821 tgcaaagtcc cttcattatt cattgctcct gttaaaacat ggtaaccata acaaccggca
     2881 cccggcaaat cgctgagtat aactccaccc gaaggatttg tgaggattgc tgctccattc
     2941 atttgcaaat ttgtggcgtt gcatccattg ctggtaaaac gtataaggcc attgttggtc
     3001 caaccataat tgaatgatat aacagagctg ctgttttggc cattcaacaa taaatacgaa
     3061 ttagcattag taaaaaaaat gcctgcataa atattggccg agtgcgaaac aataatggta
     3121 tccctgttct ggatgtttgc attgatggta ttgccataat taaattgcag ctgtgcatta
     3181 ttaagcataa agagctggtt cgaatttaac gagctgtaat cgaaaaaagt aaatgcatcg
     3241 ggcatttcag ttacattcat atgtgctgcc gtttgataat aataaatatt tcctgttgtg
     3301 ggcggagtgc ctgttccgtt tattattgta ctgccaccat tgtaattaaa agttcctcca
     3361 tgaacatata attgtttggt tttactaatg ataattgtac cagtattttc acctgtttgt
     3421 gcattgagca gccaatgcaa atcatagttt acatttatag ttccctggtt aataatagat
     3481 ccctgcattt ggttataacc ataacatccg gcaccggggt cgctttggat aagtcctgtt
     3541 gatgcattaa taattgttcc tacgttgcat tgcaaatttg tgccgttgca gccattgctc
     3601 aaaaaatgta tttttccatt attggtaaat cctgctgtag ttgcaaaact tgaatatcct
     3661 acagctcccg aaaaaagcag cactgcattg ttcgatgtgg tgagagcacc actcatcacg
     3721 ttataattgt tttgtatttc aaatgtatcg gtattgaata ttgcagcggt aatggtatta
     3781 ttataattga atactatttt ttttgtgctt gttgttggta aaaaagttga cgctaaagta
     3841 gagccataaa atgtcatgta tatagccggc aatgtagcca catccatatt tgcaattgaa
     3901 gactcgaaat acatggttcc taatcctgta acgatgggcc cgctccaatt tactgtggcg
     3961 gaagtaaaat tcaacttgct taaataattg ccaaaattga tagcgccagt gtttggcaaa
     4021 ttgataacac ctgtttttac ggtaaccgtt acacctgccg caatatttat tacacccgta
     4081 tttaccgatg cccctgagct tacatcccag tttgtagtgt aattaaaatt tattgtccct
     4141 tgattgttta gccaatgttg agattgtaat atccatagca tgccgggtta gggttgatgg
     4201 taataatgcc tgtgggtgga tttattaaca cccctgtggt gattgcaagt gttgccaagt
     4261 agcagccagg actggacatt aatatttttc cagtgttggt ccagccggtg gcaaatgtga
     4321 gtggtgctgc tgcctgtggc gggaaatgta aatcagagtg gggtgtggag gggggagggg
     4381 ggg
//
LOCUS       DAORQA010000146         4342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4981_length_4342_cov_10.854210, whole genome shotgun sequence.
ACCESSION   DAORQA010000146 DAORQA010000000
VERSION     DAORQA010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4981_length_4342_cov_10.854210"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PK736_07095"
     CDS             <1..344
                     /locus_tag="PK736_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRC33194.1"
                     /translation="FASEIKAIIEDETVKRELNEQGFYDYFTFLTVPAPNTLFGNIYK
                     LKPGHYIKIENGKVSEQTEYSDIYDNVEYHGNKTQERIQEELIDLLRTSVKYRLEADV
                     PVGVFLSGGVI"
     gene            346..1452
                     /gene="neuC"
                     /locus_tag="PK736_07100"
     CDS             346..1452
                     /gene="neuC"
                     /locus_tag="PK736_07100"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HRC33195.1"
                     /translation="MRIGILTSTRADFGIYLPLIKAMMNDAFFEIKIIAFGTHLSRQH
                     GYTIDNIINQKIKVAYKLQTLPKGDTPFDIANCMAETTKTFSKFWNKHALEFDYVMCL
                     GDRYEMFAAVCAAIPFNIKLIHLHGGETTLGAIDNTFRHCITAASTIHFTSSSAHSKR
                     VSQIINSKENVYNVGALSLDNLKILPLLSAEEFKNKFAIDLNKSSVLFTFHPETVNHA
                     KNKFYITEIISALKKLKIQIIITAPNNDTGSEVIVNALKTFVSKNKNAFWVSNFGTQG
                     YFTCLSHCKFVMGNSSSGILEAASFGKYVINLGERQRGRMAGKNVLNCNIDENEITGL
                     INKISGLPKLTKHNVYGNGNTSQKIISILKDIHE"
     gene            1445..2065
                     /locus_tag="PK736_07105"
     CDS             1445..2065
                     /locus_tag="PK736_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HRC33196.1"
                     /translation="MNNRNVILIGYSGHAYVAAEIFLLMKYKVVGYIDKIENTNNPFG
                     LEYLGNDETFYTKKINGTKLFTAIGDNALRNKLSEKYAAQGFEFTNAIHPSAILSKNI
                     TISTNILIGAQCVIQSGSVIQEGVICNTGSIIEHECTLKKYAHIAPSSTLCGNVTIGE
                     KSLIGAGTIVIPNTVIGNNVIVGAGSVIIKNIDDNKKVAGNPARTI"
     gene            2062..3108
                     /locus_tag="PK736_07110"
     CDS             2062..3108
                     /locus_tag="PK736_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRC33197.1"
                     /translation="MIDIEKNKIYQHQSTRDALAKLNLLNIHLTLFVLDANNKMLGTV
                     TDGDIRRGLLNGLTIDMPVSDFMFRDFRFFGQGNISTEQFISFKNDKLKIVPVLDANR
                     NIVEILDLTYFISYLPLDVLLMAGGEGIRLRPLTEQTPKPLLPVGGKPLLDHNMQRLK
                     KFGIKNFNISVRYLAEKIEKHIGSGSEKNIRIKYLRETTPLGTIGCAGLIKEFNNDYL
                     LVMNADLLTDIDFEQMYLKLLTEKADMIVATIPYTVNVPYGVIETDNDNVTGISEKPS
                     LLFNTNAGIYIIHKKLLSEIPYNTFFNATDLLTKVIEKKHKLIYYPIHTFWLDIGRIE
                     DYNRANEMFKFLQF"
     gene            3127..>4018
                     /locus_tag="PK736_07115"
     CDS             3127..>4018
                     /locus_tag="PK736_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRC33198.1"
                     /translation="METKNKYTELKFLVVGLGSMGKRRIRNLQQLEIKNIDGFDLRSD
                     RKEESKSKYGVTIIDNVEEAVSKNNYQAIIISVPPAKHHLYIKIALKYSLHFFVEASV
                     LDTGIAEAIEEIKTKKIVAAPSSTLFFHPAVKIIHALISQNAIGKTSHVTYHSGQYLP
                     DWHTYESVSDYYVSHKETGGAREIVPFELTWLTKLFGWPQFVSGINVKTINIPGAETI
                     DDTYHILLHEENYNLSLTVDTVSRYAVRNLLINGSDGMIKWTWENNYVEIFSASNNEW
                     NKVEFKKENAQPGYNENIIEN"
     assembly_gap    4019..4031
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1527 a          787 c          795 g         1220 t
ORIGIN      
        1 attttgccag cgaaattaaa gccatcattg aagacgaaac ggtaaagcgc gaattaaacg
       61 aacaagggtt ttacgattat tttacttttc tcactgtgcc tgcccccaat actttgttcg
      121 gcaatattta caaactcaag cccggccatt atataaaaat tgaaaacggc aaagtgagtg
      181 agcaaaccga atactcggat atatatgata acgtagaata ccatggtaac aaaacgcagg
      241 agcgtataca ggaagagtta attgaccttc tgcgcacttc ggtaaagtat cgtttggagg
      301 ccgatgtgcc tgtgggtgtt tttctgtcgg gtggagtgat ttaaaatgag aattggaata
      361 ctgacttcta cacgtgccga ctttggaatt tatttgcctt tgataaaagc aatgatgaac
      421 gatgcatttt tcgaaataaa gataattgct tttggcacac atctttcccg acaacatgga
      481 tatacgatcg acaatattat caatcaaaaa ataaaagtgg cttacaaatt gcaaacatta
      541 ccgaaaggag ataccccttt tgatattgcg aattgtatgg cagagacaac aaaaacattt
      601 tcgaaatttt ggaataaaca tgctttggag tttgactatg ttatgtgcct tggcgaccgt
      661 tacgaaatgt ttgctgctgt gtgtgcggct attccattca acattaaact gatacacttg
      721 catggtggcg aaaccacatt aggggctatt gataatactt tcaggcattg tattactgcc
      781 gccagcacaa tacactttac ctcatcctca gcccactcta agcgtgtatc gcaaattatc
      841 aactcgaaag aaaacgttta caacgtaggt gcactctcct tagataatct aaaaatactc
      901 cccttactgt cggcagaaga attcaaaaat aaatttgcca tcgatttaaa taagtcttct
      961 gtgctgttta cgtttcatcc cgaaactgta aatcatgcta aaaataaatt ttacattact
     1021 gaaattatta gtgcattaaa aaaattaaaa attcagatta ttattactgc cccaaataat
     1081 gataccggtt ccgaagtaat tgtaaatgcg ctgaaaactt ttgtatcaaa aaataaaaat
     1141 gctttttggg tttcaaactt tggaacacaa ggttacttca cctgcctcag tcattgtaaa
     1201 tttgtgatgg gcaattcatc gagcggaata ctggaagcgg catcgtttgg caagtatgta
     1261 atcaatcttg gcgaaagaca aagagggcgc atggcaggca agaatgtatt aaattgcaat
     1321 attgatgaaa acgaaataac cggtttgatc aacaaaatat caggtttgcc taaattgaca
     1381 aagcacaacg tatatggtaa tggtaacaca tcgcaaaaaa taatttcaat ccttaaagac
     1441 attcatgaat aacaggaacg taatattaat aggatactcg ggccatgcat acgttgcagc
     1501 agaaatcttt ttgttgatga agtataaagt ggtaggctat atcgacaaaa ttgaaaacac
     1561 caacaatcca tttggattag aatatctggg caatgatgaa acattttaca cgaaaaaaat
     1621 taacggcaca aaacttttta cagcaattgg cgacaatgcg cttcgcaata aattatcaga
     1681 aaaatatgct gcacagggtt tcgagtttac aaatgctatc cacccttcgg caatcttatc
     1741 gaaaaacatt actatttcaa caaacatact tataggagcc cagtgtgtaa tacaaagcgg
     1801 cagtgtgatt caggaaggag ttatttgcaa tacaggaagt attattgagc atgaatgcac
     1861 cctaaaaaaa tatgcccata ttgcgccctc atcaacgctt tgtggaaatg taacaatagg
     1921 agaaaaatca ctcataggtg ctggtactat tgtaattcct aatactgtaa ttggcaacaa
     1981 tgtaattgta ggtgccggca gtgttataat taaaaatatt gacgataata aaaaagtagc
     2041 aggtaaccca gctcgaacaa tatgatcgat atagaaaaaa ataaaatata ccaacaccaa
     2101 agcacaagag atgctttggc gaaacttaac ttactgaata tacaccttac cctatttgtg
     2161 ctggatgcca ataataaaat gttgggcact gttaccgatg gtgatatacg aagaggacta
     2221 ctcaacggcc tcaccatcga tatgccggtg agcgatttta tgtttcgcga ttttcgtttt
     2281 tttgggcaag gaaatatttc tacagaacaa tttatttctt tcaaaaacga caaattaaaa
     2341 atcgttccgg ttttagatgc gaatcgtaac atagtcgaaa ttcttgatct tacttatttc
     2401 atatcatatc tccccctcga tgtattatta atggcaggtg gcgaaggcat tcggttaaga
     2461 ccgctaaccg aacaaacgcc aaaaccactt ttaccggttg gtggtaaacc tttgcttgat
     2521 cacaatatgc agcggttaaa aaaattcggt atcaaaaatt tcaacatctc tgtacgctac
     2581 ctggccgaaa agattgaaaa acatataggc agcggcagcg aaaaaaatat ccgcataaaa
     2641 tatttacgcg aaacaactcc gcttggcact attggttgtg caggattaat aaaagaattt
     2701 aataacgatt atttattggt gatgaatgcc gacttgctca ccgatataga ttttgagcaa
     2761 atgtacctca agttactaac cgaaaaagct gatatgattg tggccaccat tccttacacg
     2821 gttaatgttc cttatggcgt aatagaaacc gataacgata atgtgacagg cataagtgaa
     2881 aaaccttcgt tgctatttaa caccaacgca ggaatttata ttattcacaa gaaactattg
     2941 agcgaaattc catacaacac ttttttcaat gctaccgatt tgcttactaa ggtgattgaa
     3001 aaaaaacaca aattaatcta ctaccccatt cacacctttt ggctcgatat aggaagaata
     3061 gaagattaca accgcgcaaa cgaaatgttt aaatttctac aattttaaaa atcaaattag
     3121 cacaggttgg aaacgaaaaa taaatatact gaacttaaat ttttagttgt gggtttgggc
     3181 tcgatgggca aacgcagaat acgcaacctg cagcaattgg aaataaaaaa tattgatggt
     3241 ttcgatctgc gcagtgaccg gaaggaagaa tcaaaatcga aatacggtgt aacaataatt
     3301 gacaatgttg aagaagcagt ttcgaaaaac aattatcagg caatcattat ttcggttccg
     3361 ccagcgaaac accatttata cattaagatt gcacttaaat atagtttgca cttttttgtg
     3421 gaagccagtg ttttagatac cggaatagca gaagccattg aagagattaa aacaaaaaaa
     3481 attgttgccg cgccatcatc cactttgttt tttcatcctg cagtaaaaat aatacacgca
     3541 ttaatttcgc aaaatgccat tggcaaaaca agccatgtaa cctatcactc aggacaatat
     3601 ttacccgatt ggcataccta tgaaagtgtg agtgattatt atgtcagcca caaagaaacc
     3661 ggaggcgcgc gcgaaatagt tccattcgaa ttaacatggc tcacaaaatt attcggttgg
     3721 ccacagtttg tatcgggtat taatgtaaaa acaataaaca ttcccggtgc cgaaactatt
     3781 gacgacactt atcatatatt actgcatgaa gagaactaca atctatcact tacagttgat
     3841 actgtatcgc gctacgctgt gcgcaacctg ctcattaatg gaagtgacgg aatgataaag
     3901 tggacttggg aaaataatta tgtagaaata ttttctgcat caaacaatga atggaataaa
     3961 gtagaattta aaaaagaaaa tgctcaaccc ggttacaacg aaaacataat tgaaaatann
     4021 nnnnnnnnnn ngtagaagaa ctgcaacagt ttattgatac aacacttgga acaacaacat
     4081 ttttcaatac gatggaatac gattttgtga ttcttcaatt attgtacact attgaaaaat
     4141 cgtggcgcac gaaaacaatt ttgccttgcg ataaaacatc tttaatcaac aataaatgaa
     4201 aacaggattg tgcatagttg cccgtgtaga atcaacccgg ttgccgcgaa aacattttat
     4261 tgatgttgaa ggcaaatgct ttattgaatg gctgcttcta cgcatgactc atcatttcga
     4321 aagtcaaata aaaaaatata at
//
LOCUS       DAORQA010000147         4334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_4991_length_4334_cov_7.202384, whole genome shotgun sequence.
ACCESSION   DAORQA010000147 DAORQA010000000
VERSION     DAORQA010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4991_length_4334_cov_7.202384"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..547)
                     /locus_tag="PK736_07120"
     CDS             complement(53..547)
                     /locus_tag="PK736_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33199.1"
                     /translation="MKHLLRIQLWLLIMFGYSCHAQQAGYTSQSKGAIKSYEAAIKLY
                     DTRQNDMALKELETAIRRDSMFIEAYMLRAAIYADMEQYDNASLALRKAVEIHPTFFP
                     NNFYSLGRFQMMLSQYDAAKKYFGQFVQMKEIGRDLKDESLFFIKCCDFAVDAMQHPV
                     PFEP"
     gene            complement(552..1151)
                     /locus_tag="PK736_07125"
     CDS             complement(552..1151)
                     /locus_tag="PK736_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HRC33200.1"
                     /translation="MNNYAVMEHFYTVQGEGSNTGKAAYFVRLGGCDVGCVWCDVKES
                     WNAADHPQYTTPQITEWITSAGAHNAVITGGEPCMYNLDELTATLKNQRIQTWLETSG
                     AHPVTGQWDWICVSPKKFKAPLPQSLLKADELKVIIFNNTDFEWAEQHASQVNQNCKL
                     FLQPEYSKIDKMLPSIIEYVKGNPKWTISLQTHKYMQIP"
     gene            1565..2482
                     /locus_tag="PK736_07130"
     CDS             1565..2482
                     /locus_tag="PK736_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC33201.1"
                     /translation="MRTENEIVSLKNISKSFGNFNAVDEISLTVQEGDVYGFLGPNGA
                     GKSTTLRMMLGLIFPTKGEVNIFGLPFNRNRSQILKQIGAIIEKPDFYNYLPADKNLK
                     LFSQISGNVKEIKQIHAMLDFVGLQGRYYHKVKTYSHGMKQRLGLAQALLNDPRLIVL
                     DEPTTGLDPQGIIDIRNLILHLSRDKNITVVLSSHILSEIELIASRMVIINKGKKIAE
                     GKVSELLNEQQLQVYFDVDNTQKALQVLQNVSLNISGVHEKENMVVLQTSQNNISTVN
                     KLLVDSGVNVSGINQKRPLEELFLKLTND"
     gene            2497..3339
                     /locus_tag="PK736_07135"
     CDS             2497..3339
                     /locus_tag="PK736_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRC33202.1"
                     /translation="MFFNLLQAELYKIYSKPRSYIGFAALFLISALIHFALYADGKSY
                     INIVTQSLQQSFAVEGNILNGNLVCYIILQMLIIHVPLLIALVTGDLMSGEGASGTLR
                     LLVLKPVSRMQIFIAKFIAGSFYTATILIFLAIVAWGLGILFFGKGDLMILRSEEITI
                     LPANDISWRFFAAFGAAFIALTVVSAISFMLSCFSNNSVGPIISTMAIIILFTIIATL
                     DLPSFQTIHPYMFTTHLVAWHMFFENPVPYHEILNSMLILLLHIVGCLGIAAWHFTRK
                     DILV"
     gene            3345..>3534
                     /locus_tag="PK736_07140"
     CDS             3345..>3534
                     /locus_tag="PK736_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33203.1"
                     /translation="MKNTKKIIFVLCCCATLLLQNARGQDGATLLKSAEKKFNAVKNY
                     KADVNIKTDVSFIKVVPVN"
     assembly_gap    3535..3544
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3545..4073
                     /locus_tag="PK736_07145"
     CDS             <3545..4073
                     /locus_tag="PK736_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33204.1"
                     /translation="FKTNKRNVPDAPSPDKMHIECKGIAILPKQGPDFLFATLLNSEN
                     FIAVDMGTQLYNTIILKIIKLLPKSDTSDLVLGTVWIDASTLLIYKTQVTTKSRGTVD
                     LEFTYGKYANYLLPDKVVFEMDVNKFKIPKAIAADYESSAQKQKNAKQAKRGKVTITY
                     KSYILNNGIDDSVFK"
BASE COUNT         1316 a          783 c          835 g         1390 t
ORIGIN      
        1 acattgttgg gtaatattcg tttgccgaag tgttgataac tccacccata ttctatggtt
       61 caaaaggtac aggatgttgc atagcatcga cagcaaaatc gcagcacttt ataaaaaata
      121 acgattcgtc ttttaagtcg cgtcctattt ctttcatctg cacaaactgt ccgaaatatt
      181 ttttcgctgc atcatattgc gaaagcatca tttgaaatcg cccaagcgag taaaaattat
      241 ttgggaagaa agtgggatga atttcaacag cttttcttaa tgccaaagaa gcattatcgt
      301 attgctccat gtcggcataa atagccgctc gcaacatata ggcttcaata aacatgctgt
      361 cgcgcctgat tgcagtttcc agttctttaa gcgccatatc gttttgacgg gtgtcgtata
      421 atttgatggc ggcttcatag ctttttatag cccctttcga ttgcgaagtg tatcctgcct
      481 gttgcgcatg gcacgaatag ccgaacatta tcagcaacca taactgtatt cgtagtaagt
      541 gtttcattct attaaggtat ttgcatgtac ttgtgggttt gtaacgaaat agtccacttg
      601 ggattgcctt tcacatattc gattatcgat ggcaacattt tatcaatctt gctgtattcg
      661 ggttgtaaaa ataatttgca attttgattt acttgcgatg catgctgctc ggcccattca
      721 aaatcggtat tgttaaaaat aataacttta agttcatcgg cttttaatag tgattggggt
      781 aggggtgctt taaacttttt tggcgataca caaatccagt cccattggcc tgttacagga
      841 tgtgcccctg aagtttctaa ccatgtttgt atacgctggt tttttaaagt agcagtaagt
      901 tcatcaagat tatacataca gggctcgcct ccagttataa ctgcattgtg tgctccggca
      961 cttgttatcc actctgttat ttgtggggtt gtatattgcg ggtggtcggc tgcgttccac
     1021 gattctttaa catcacacca cacacatcct acatcgcaac cgcccagcct tacaaaataa
     1081 gctgccttgc cggtattgct tccttcgcct tgcacggtat aaaagtgttc catcaccgca
     1141 taattattca tcgtttgctt tcagtttatt cgcaaataaa gcaataactt ttcgatgttt
     1201 agagtgttgt atgatgaaca aattatttta aatttattta aaagtgtatt agttgatttt
     1261 ttgaacttaa tttatgtggc atgttattat aatatgttac acaaaggtac acaaaggagg
     1321 aaagaagttg cacaaagaat tctttgatgg tttataataa atgtgttaga cgaatgaata
     1381 caaataaggg atcaagttgc attaagaaaa caggaaggtt gataaaacaa gaaaataaaa
     1441 tctacctatc tttttaaagc atattgacca gttttataaa aagtgttttt ttattttgaa
     1501 gaaaaagttt gtatgcaatc aataaaaatg aattcattta caccgccaaa ttaagtatcg
     1561 aattttgaga acagaaaacg aaatagtatc gctaaaaaac atcagcaaaa gttttggaaa
     1621 ttttaacgct gtagatgaaa taagtctcac ggttcaggaa ggtgatgtat atggctttct
     1681 aggacccaac ggtgcgggca aaagtaccac attgcgcatg atgttgggat tgatttttcc
     1741 tacaaaagga gaagtgaata ttttcggcct gccatttaat agaaatcgtt cgcaaatatt
     1801 gaaacaaata ggggcgataa tagagaagcc cgatttttac aactacctgc ctgccgataa
     1861 aaatctcaaa ttgttttcgc aaatttcggg aaatgtaaaa gaaataaaac agatacatgc
     1921 catgctcgat tttgtaggat tgcaagggcg ctattatcac aaagtaaaaa cgtattcgca
     1981 tggaatgaag caacgtttag gtttagcgca agcgcttcta aacgacccac gccttattgt
     2041 gctggatgaa cctaccacag gcctcgaccc gcagggtatt atcgatatcc gaaatttaat
     2101 tttacatctg agccgcgata aaaatattac tgttgtttta tcctcacaca ttttatcgga
     2161 aatagaattg attgcttcgc gaatggtaat tattaataaa gggaaaaaaa ttgcggaggg
     2221 caaggtgagt gagctactaa atgagcaaca attgcaagtg tattttgatg tcgacaatac
     2281 acaaaaagca ctgcaggttt tgcaaaatgt ttctttgaat atttcaggcg ttcatgaaaa
     2341 agaaaatatg gtggtacttc aaacttcgca aaataatatt agcacagtaa ataaattact
     2401 tgttgatagt ggagtgaatg tgagtggcat aaatcaaaag cggccgctcg aagaattgtt
     2461 tttaaaactc acaaacgatt aaattaatta tacaccatgt ttttcaattt gctacaagcc
     2521 gaattatata aaatttactc caagccccga agttatattg ggtttgctgc tttgtttctt
     2581 atttctgccc tcatacattt tgcgttatat gccgatggta aaagttatat aaacattgtc
     2641 actcaaagcc tgcagcaatc ctttgcagtg gaaggcaata tattaaatgg caatctggta
     2701 tgctatatta ttttgcaaat gttgataata catgtgcctc tgctaattgc gctcgtcaca
     2761 ggcgatttaa tgtcgggcga aggagcatcg ggcacattgc gtttattagt tttgaagcct
     2821 gtatcgcgca tgcaaatatt tattgccaag tttattgccg gcagttttta cacagcaact
     2881 attttgattt ttcttgccat agttgcctgg ggtttaggaa tactattttt tggcaaaggt
     2941 gacttgatga ttttgcgtag cgaagaaatt accatactcc ctgctaacga tatttcgtgg
     3001 cgtttttttg ccgcatttgg tgccgcattt attgcgttaa ctgttgtgtc ggcaatttct
     3061 tttatgcttt cttgcttctc caataattct gtagggccta ttatttcgac tatggccatt
     3121 attattttgt ttaccattat cgccacactc gatttgcctt cctttcaaac cattcatcca
     3181 tatatgttta caacacattt agtagcatgg cacatgtttt tcgaaaaccc tgtaccgtac
     3241 cacgagattt tgaattctat gttgatttta cttttacaca ttgttggatg ccttggcatt
     3301 gcagcatggc attttacccg aaaagatatt ttagtttaat agaaatgaaa aacaccaaaa
     3361 aaataatatt tgtgctttgt tgttgcgcaa cactgttgtt gcaaaatgca cgtgggcaag
     3421 atggggctac tttgcttaaa agtgcagaga agaaatttaa tgctgttaaa aattataagg
     3481 ccgatgtgaa tattaaaacc gatgtaagtt ttataaaggt tgtgcctgta aatgnnnnnn
     3541 nnnncttcaa aactaataaa cgcaatgtgc ccgatgctcc ttcgcccgac aaaatgcata
     3601 ttgaatgcaa aggaattgca atattgccaa agcaaggccc cgattttttg tttgctactt
     3661 tgctcaactc cgaaaatttt atcgctgttg atatgggtac tcagctttat aatactatca
     3721 ttttaaagat tataaagcta ttacccaaaa gtgacacttc cgatcttgtc cttggtacag
     3781 tatggattga tgccagtaca ttgttgatat acaaaactca ggttactaca aagtcgcgcg
     3841 gcacagtcga cctcgaattc acttatggta aatatgccaa ctatcttttg cccgacaaag
     3901 ttgtgtttga aatggatgtc aacaaattta aaattccaaa ggctatagct gccgactatg
     3961 aatcatcggc acaaaaacaa aagaacgcaa aacaagcaaa gcgaggcaag gttactataa
     4021 catataagag ctatattttg aacaatggga tagacgatag tgtttttaaa tgagtttgtt
     4081 gcggggggaa gtttggaaag ttgagaaagt acaaagttag agaggttcaa agttgggaaa
     4141 gtttgtaagt tcttactacg aatgcgccag ccatggtttg tgtccccact aaccatcagc
     4201 acgatatctt tgcatttcca attctgcgcc atacattgtt tgtgtcccca caaaccatcc
     4261 gcacgatacc atctcgaaac accacaggtt taaaaccgtt gctacaatat gcttcaacat
     4321 tatggttttt ttat
//
LOCUS       DAORQA010000148         4316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5015_length_4316_cov_10.143863, whole genome shotgun sequence.
ACCESSION   DAORQA010000148 DAORQA010000000
VERSION     DAORQA010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5015_length_4316_cov_10.143863"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..597)
                     /locus_tag="PK736_07150"
     CDS             complement(172..597)
                     /locus_tag="PK736_07150"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HRC33205.1"
                     /translation="MAKKVNKLVVDYEGEFEFTLLGISCAHKDYRLCYALNKVLDIEF
                     IKAKNHELKLAKQTSVFMFGKYVATNEYGRKIFLINNKQDDNILLPELKMLDYILMFE
                     PKYDGDKGKLLQEIKDIKIVQTVVELDVQKIKSKHNLLY"
     gene            complement(651..1247)
                     /locus_tag="PK736_07155"
     CDS             complement(651..1247)
                     /locus_tag="PK736_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRC33206.1"
                     /translation="MRFNARLLFLFTGVIPSVEGKQYLNKNKTYVFCINHSSYIDIVT
                     CYVAIAQHFHMMGKAELNDIPMFNIFFKKMNIPVNRGSITESHKAYQRACNDIESNIS
                     IIIFPEATIPADAPKLGNFKNGPFKLAIEKKVPCVPVTFCTNYKLLPDNILFRKFGGR
                     PGICKVIIHQPIDTQNMVDADVSKLKQTVFDIIKNDLP"
     gene            complement(1657..2769)
                     /locus_tag="PK736_07160"
     CDS             complement(1657..2769)
                     /locus_tag="PK736_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33207.1"
                     /translation="MSSEHFIFQGTPHEHPLNFKYSVIDINLDNGLGGIDSNYKSLIL
                     LNDTMVSGFTNACRHGNGRDWWVISPRFNSNAHKKFMLTPDSVYFSSSQNIGDTIRWR
                     AGGQSCFRPDGTMYAIKDIISGINLFKFDRCTGMFYDSVFFQVPKLLGSYLDWTNGLA
                     FSPSGRFLYVCQYTKIWQYDTWSSNIAASQIQVAEWDTVYNPFETYFFNMQLAPDKKI
                     YISTWGTNYSLHTIENPDSLGMACNVNQNSVSLSFPNSSVPTFPNYDLGPLIGSPCDT
                     LGLGVLQNNSMEQLEITVSPNPANSIANFVYHLPTNKQVTLTLYDGYGNIVARQSLYG
                     VFRNFMFHTDKLANGLYYWVAQNGEVSASGKLVVVH"
     gene            2786..4189
                     /gene="pyk"
                     /locus_tag="PK736_07165"
     CDS             2786..4189
                     /gene="pyk"
                     /locus_tag="PK736_07165"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRC33208.1"
                     /translation="MIATIGPASSSYEVLNKMMDAGVNVCRLNASHGNHEIQQEIIDN
                     IRLLNEERQSHVAILYDLQGPKIRVGEVENNGVELINGNTITLTINDCIGTAEKVYVK
                     YPSFLQDVKTGDRILIDDGKLELKILSNNNIDSAVAEIINGGTLSSKKGLNLPNTRIS
                     LPSLTEKDKVDLEFALENNVEWIGLSFIRSAKDIEALKNIIKYKNKTARTIAKIEKPE
                     AVVDIDNIIAVSDGVMVARGDLGVEIPMEEVPLFQKMIVKKCIAAAKPVIIATQMMES
                     MITSQRPTRAEVNDVGNSVLDGADAVMLSAETSVGKDPVNVVEHMYRIIRTVQNDSGI
                     YFHENAPQRESATFISDSTTYNACVIAKQVGATGITTMTHSGYSAIKIASHRPNCDIL
                     VFTDNKLLLTQLNLVWGVRGFFYDKYENTDTNITDIKNLLVQKGLVKPGNIVIHVYST
                     PLMARGRANSLKINVID"
BASE COUNT         1396 a          787 c          797 g         1336 t
ORIGIN      
        1 cttcgtaact ggaagatgca ggaccaatag ttgctattat ttttgttttg ttattacaca
       61 tcaggtttta tttatctgta tattttaaat tgttaatcta atttcgaaag agtgagttgt
      121 ttttaattta tcatttaaaa aattttgctt ttaatttaga ttcaacattt attaatacaa
      181 taagttatgt ttcgatttta ttttttgtac atctagttca accaccgttt gtactatttt
      241 tatatctttt atttcttgaa ggagtttacc cttatcgcca tcgtacttcg gttcaaacat
      301 caaaatataa tcaagcattt ttaattcagg caataatata ttgtcgtcct gtttattatt
      361 tatcagaaat attttacggc catattcatt tgtggcaaca tattttccaa acataaaaac
      421 cgaagtttgt ttggcaagtt ttagttcgtg attttttgct ttaataaatt ctatatcaag
      481 cactttatta agtgcgtaac acaaacgata atctttatgt gcacaactta ttcccaataa
      541 ggtgaactca aattcacctt cataatccac tacaagttta ttgacttttt ttgccattgc
      601 atgtgtgctt tattagggta tgcaaagata gtgtaaaaaa tacactttga ctaagggagg
      661 tcatttttta taatgtcgaa aacggtttgt tttagtttcg atacatcggc atctaccata
      721 ttttgagtat cgataggctg atgaataatt actttgcata tgccgggccg tccgccaaat
      781 ttacgaaaca aaatattgtc gggtagtaac ttgtagtttg tgcaaaaggt aacaggtaca
      841 cacggcactt ttttttctat agcaagttta aatggcccgt ttttaaaatt tcccaatttg
      901 ggtgcatcgg cagggatggt agcttcggga aaaataataa tggaaatatt cgattctata
      961 tcgttgcatg cacgttgata tgctttatgc gattcggtaa tgctgccgcg atttacggga
     1021 atattcattt ttttaaaaaa tatattgaac ataggaatat cgttcaactc agctttgccc
     1081 atcatgtgga aatgttgtgc aatggcaaca taacatgtaa caatatctat atacgatgaa
     1141 tgattgatac aaaaaacata agtcttattt ttatttaaat attgtttccc ttccacactg
     1201 ggtattactc ccgtaaacag aaaaagtaaa cgggcattaa agcgcattaa tttaaatgcg
     1261 gcaggaaaac tttttcggtt gtttagtaaa agcacgagta gaggataatg gattacaaac
     1321 aataaaatac caataaaaaa aaaccaccac cgccaaatgt ttccaagaat aattttaaaa
     1381 aggtttgtca aaatattttt tatttttgat tacaacacaa atgtagcaaa tgtatgcagc
     1441 ctttataata gcaatgtaaa caggaataca ataatatttt gttaagaggc taattggtat
     1501 tatttctttg tttctaattg aataggcaac caaatggctg aaccccgcca cttcactctg
     1561 ggcagccatg atttatttga tttaattatt aatgtaattc attttcaacg caagatgata
     1621 ttttttttgc cttaacaact ttcataactc gccccgctaa tgcacaacca ccaatttccc
     1681 acttgcacta acctccccat tttgcgccac ccaataatac aaaccatttg caagcttatc
     1741 ggtatgaaac ataaaattac gaaacacgcc atacaatgac tgccgtgcca caatattgcc
     1801 gtaaccatcg tatagggtaa gggttacttg tttgttagtg ggtaggtggt aaacaaaatt
     1861 tgcaatgctg ttggcggggt tggggcttac agttatttct aattgctcca tgctgttgtt
     1921 ttgtaaaacg cccaagccaa gcgtatcgca tgggctgcca ataagtgggc ctaaatcgta
     1981 attgggaaaa gtaggaactg aagaattggg aaacgataat gaaacagaat tttgatttac
     2041 attacaggcc atacctaatg aatctggatt ttcaattgta tgtaaagaat agtttgtacc
     2101 ccatgtggaa atataaattt ttttatcagg ggctaactgc atgttaaaaa aataggtttc
     2161 aaatgggttg taaacagtat cccactcagc cacttgaatt tgcgatgctg caatattgct
     2221 agaccaagta tcatattgcc aaattttggt gtattggcat acatacaaaa aacgcccact
     2281 gggtgaaaat gccaatccat tagtccaatc taaataacta ccaagcaatt tgggtacttg
     2341 aaaaaaaacg gaatcgtaaa acattcctgt acatctatca aacttaaata aattgatgcc
     2401 actgataatg tcctttattg catacatagt gccatccggt ctgaaacaac tctgccctcc
     2461 cgctctccat cttatggtat cacctatgtt ttgcgaacta ctaaaataaa ctgaatcagg
     2521 agtaagcata aattttttgt gagcattgga attaaaccgt ggcgaaatca cccaccaatc
     2581 gcgtccattg ccatgtctgc aggcgttggt gaaacctgac accattgtat cattaagtaa
     2641 aattagcgac ttataattac tatctattcc accaaggcca ttatccaaat taatatctat
     2701 tactgaatac ttgaagttta aaggatgctc atgtggtgtg ccttgaaaaa taaaatgttc
     2761 tgagctcatg tgaaacaaaa caaaaataat agcaactatt ggtcctgcat cttccagtta
     2821 cgaagtgctt aacaaaatga tggatgccgg agtaaatgta tgccgactaa acgcctcgca
     2881 tggcaatcac gaaatacagc aggaaataat agataatatc cgtttgctta acgaagaacg
     2941 acagtcgcat gtggcaatat tgtacgattt gcaaggccca aaaatacgcg tgggcgaagt
     3001 ggagaacaat ggtgtggaat tgataaacgg caacactata acccttacta taaacgattg
     3061 tataggcact gccgaaaaag tgtacgtaaa gtacccttcg tttttgcagg atgtaaaaac
     3121 gggcgatcgt attttgatag atgatggcaa gctggaactg aaaatattat cgaacaataa
     3181 cattgattcg gctgttgccg aaataataaa tggcggaaca ttgtcatcta aaaaaggact
     3241 caacctgccc aacacacgta tttcgttgcc atcgcttacc gaaaaggata aagtcgattt
     3301 ggagtttgct cttgaaaata atgtggagtg gattggcctt tcatttatac gttcggcaaa
     3361 ggatattgag gctttaaaga atattataaa atacaaaaac aaaaccgcac gtactatagc
     3421 aaaaatagaa aagcccgaag ccgtagttga tatcgacaat attattgctg tgagcgatgg
     3481 agttatggtg gcacgtggcg atctcggtgt agaaatacct atggaggaag tgccactgtt
     3541 tcaaaaaatg attgtgaaaa aatgtatcgc tgctgccaag cctgttatta ttgctacaca
     3601 aatgatggag agcatgatta ccagtcagcg gccaacacgt gccgaagtaa atgatgtggg
     3661 caattctgta ctcgatggtg ccgatgccgt aatgcttagt gccgaaacat cggtaggtaa
     3721 ggacccggtg aatgtggtag aacacatgta ccgcattatt cgcactgtgc aaaacgactc
     3781 gggaatttac tttcacgaaa acgcaccgca gcgtgaatca gcaactttta ttagcgactc
     3841 aactacgtat aatgcatgtg ttattgcaaa gcaagttggc gcaacaggaa ttaccacaat
     3901 gacacattcg ggttactcgg ccattaaaat tgccagccac cgacccaatt gcgatatact
     3961 tgtttttact gacaataaat tattgctcac acagctaaac cttgtttggg gtgtgcgtgg
     4021 tttcttttat gataaatacg aaaacaccga cactaacatt accgatataa aaaatcttct
     4081 ggtgcaaaaa ggtttagtga aacccggcaa cattgtaata catgtttact ccactccgct
     4141 tatggcacgt ggccgcgcaa actcgctgaa gattaatgtt attgactaaa ttcttgtcat
     4201 tggtgaattc ttgtcattgg tcattatttt gaggattgtc attgggattc aaaaaatcgg
     4261 aatcgcaatg accaatgcct attgacaaga atcacttcac cacactacca ttacca
//
LOCUS       DAORQA010000149         4293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5048_length_4293_cov_4.199858, whole genome shotgun sequence.
ACCESSION   DAORQA010000149 DAORQA010000000
VERSION     DAORQA010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5048_length_4293_cov_4.199858"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK736_07170"
     CDS             complement(<1..267)
                     /locus_tag="PK736_07170"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HRC33209.1"
                     /translation="MCTVTFLPLSNQNFILTSNRDEDVNRAQALPVAQYQHYDTNIFY
                     PKDGKANGTWIAASTTGITLCLLNGAFVKHSFGQTYRMSRGIVLL"
     gene            complement(272..778)
                     /locus_tag="PK736_07175"
     CDS             complement(272..778)
                     /locus_tag="PK736_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33210.1"
                     /translation="MLHLIAKKYLTDLANVLHQIKDNDYCRPIAVFDNGSVGKHVRHI
                     LEFYKCLIIANKNNDVICYDNRERNLLIEENVRFAIDNITEIIDDISSIEQNKRMLLK
                     SVYNDAEINTETTLLRELTYNIEHTVHHLALIRIGIVAECSYIKIDKTFGYADSTVQY
                     LKTLQAVS"
     gene            complement(933..1619)
                     /locus_tag="PK736_07180"
     CDS             complement(933..1619)
                     /locus_tag="PK736_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRC33211.1"
                     /translation="MNSDKKFWYLRNHQLYNTLSDNELDSLCIIASYKEGKKGDIINF
                     TDRENELLYSVKEGTFKICYQDDNAHEIISEILTQGDLFGRIHLSRKGNNNRDEYAKV
                     ISDTFKICSFEAEKFKEMLKNNPDLSLKYSDYISEKMESFQMKYTDLVFKDVYTRVAD
                     FYKRYAFHSAKATGNSADMEMILTHQEIADYIAASRQTVTQIINTLVAEGKIIYQGRK
                     RVIIPDMQKL"
     gene            complement(1616..2251)
                     /locus_tag="PK736_07185"
     CDS             complement(1616..2251)
                     /locus_tag="PK736_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRC33212.1"
                     /translation="MIQVLIILFCAAVVSFVGSLQLGPVNVMVINTVLYQNRRSAWLV
                     ALGGCIPEFIYSALAVYFGQHFVSNSVLNKALSIVSIILFAAMGVFFIFKKAKAISLE
                     SNSLQKANTAHNFYKGFALASFNPQLLPFWFFIFLAFNDITLLKVSSSIQKLAFVLGS
                     GLGAFALLSLFIFLTHKYKTKIISKINLTQINIAIGVFFLIIALRQLISII"
     gene            complement(2339..2548)
                     /locus_tag="PK736_07190"
     CDS             complement(2339..2548)
                     /locus_tag="PK736_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HRC33213.1"
                     /translation="MLPVRYEDLAAHPQKTINEICTFCNLSDDAKLMEYAIETLKPVP
                     SKKPVEINPLLKDAFMKIMKEMKYD"
     gene            complement(2493..3317)
                     /locus_tag="PK736_07195"
     CDS             complement(2493..3317)
                     /locus_tag="PK736_07195"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRC33214.1"
                     /translation="MVGQINSNYIRTRWTKFFSRMLSYALFEGRPVTTAGQWINPIVF
                     ANMSFAKLGLIKKKVEQPTFILGTGRSGTTILGIVMSMHRDVAFLNEPKALWHSVFKN
                     EDLIGSYTRENALYELHESDAQPEAIKKMHNIFGFYLSTVFSKRVWDKYPQLVFRMDF
                     VKKIFPDFKAIFLYRNGWDTAISSAGWSDIHGEQKGEETHNWWGVNNRKWNLLCDQIV
                     SKDIDLAPHLQEIKKFTSELDKSIVEWIVSMRFEISQPCVACSVRRPCCTSSKNHK"
     gene            complement(3415..4203)
                     /locus_tag="PK736_07200"
     CDS             complement(3415..4203)
                     /locus_tag="PK736_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC33215.1"
                     /translation="MNIDLQKKRALVCGSTQGIGKAVAITLAELECNVTLLARNENVL
                     KEVVKELPSFAGQQHDYIVGDFAFPQELKQKVAQYIDEKNAIHILINNTGGPPAGPVA
                     TATAEEFTAAFTNHLICNHLLVQECLEGMKAANYGRIINIISTSVKQPIKGLGVSNTI
                     RAAVANWAKTLSHEVARYGITVNNVLPGATNTARLTNIIEDKSKKTGRAFNDVTREML
                     AEIPAGRFAEAVEIAEAVAFLASPAAGYINGINLPVDGGRTLSL"
BASE COUNT         1316 a          819 c          772 g         1386 t
ORIGIN      
        1 gagtaaaaca atgccccggc tcatgcgata agtttgcccg aatgaatgtt ttacaaacgc
       61 cccattaagc agacaaagtg taatgcccgt tgtagaagct gcaatccagg tgccatttgc
      121 tttgccatct ttggggtaaa atatatttgt atcgtaatgc tggtattggg ctacaggcaa
      181 agcttgtgca cggtttacat cttcatcgcg attcgatgta agtataaaat tttgatttga
      241 taatggaaga aaagtaactg tacacatgcg cttacgaaac cgcttgtaag gtttttaaat
      301 attgcaccgt gctgtcggca taaccaaaag ttttgtctat tttaatatag ctgcattcgg
      361 caacaatacc aatgcgaatt aaagcaagat gatgaacagt atgttctatg ttgtaagtaa
      421 gttcgcgcaa taatgttgtt tctgtattaa tttctgcatc attataaacg gactttaaca
      481 gcatgcgttt gttttgttct attgaagaaa tatcgtcaat gatttctgta atattatcaa
      541 ttgcaaaacg cacattttct tctatcaata aattgcgttc acggttgtcg taacaaataa
      601 catcgttatt tttatttgca ataataaggc atttataaaa ttctaaaata tgccgcacat
      661 gcttgccaac actgccatta tcaaataccg ctatcggccg gcaataatcg ttgtctttta
      721 tttgatgcaa aacattagca aggtcggtaa gatatttttt ggctatgagg tgtaacatta
      781 tattcaaata ttatgtagtg atatttacaa ttctggtttg aggcgattta attttttcta
      841 aaaatacaaa ttcatttcat ataaggcaaa tgtactatgc atatgcaaaa cactatgtca
      901 cataaacgac acaagccgga gaaatgtaaa ggctataatt tttgcatatc gggaataata
      961 acgcgcttgc gaccctgata aattattttg ccttcagcca caagggtgtt aattatttga
     1021 gtaaccgttt gacgtgaagc ggcaatgtaa tcggcaattt cctggtgtgt aagtatcatt
     1081 tccatatcgg cactattgcc cgttgctttg gcggaatgaa acgcatatcg tttataaaaa
     1141 tcggctacac gtgtgtatac atctttaaaa accaaatcgg tatatttcat ctgaaacgat
     1201 tccatttttt cgctgatata gtccgaatat ttaagtgaca agtcgggatt gtttttcaac
     1261 atctctttaa atttttcggc ttcaaaactg caaatcttaa aagtgtcgct tatcactttt
     1321 gcatattcat cgcgattatt attgccctta cgcgaaaggt gaatacgtcc aaacaaatca
     1381 ccttgtgtaa gtatttcgga aataatttcg tgggcattat catcctgata acatatttta
     1441 aatgtgcctt cttttacact gtacaaaagt tcattctctc tatcggtaaa gttgatgata
     1501 tcgccttttt tgccttcttt ataagatgct attatacaca gcgaatcaag ctcattatcg
     1561 ctcagggtgt tgtataattg gtggttacgc agataccaga attttttgtc gctattcata
     1621 ttatgcttat caattgcctt aatgcaatta taagaaaaaa aactccaatg gcaatattga
     1681 tttgcgttaa attaatcttc gatataattt tggttttata tttatgtgtc aggaaaataa
     1741 aaaggctcag caaagcgaaa gcacctaaac ccgaaccaag gacaaatgca agtttttgta
     1801 ttgacgagga tacttttaaa agtgtaatat cattgaaagc taaaaaaata aaaaaccaaa
     1861 atggcagtag ttgcggattg aaagatgcta atgcaaatcc tttataaaaa ttatgtgccg
     1921 tattcgcttt ttgtaatgaa ttgctttcga gcgaaatggc tttagctttt ttaaaaataa
     1981 aaaacacacc cattgctgcg aacaatataa tactaacaat gctaagtgct ttattcaaca
     2041 cactgttgct tacaaaatgt tgcccaaaat aaactgccaa tgcgctgtat ataaactcgg
     2101 gaatgcaacc acctaatgcc acaagccaag ctgaacgtct gttttggtat aacacagtat
     2161 ttatcaccat cacatttaca gggcccagtt gaagcgaacc aacaaagctt accacagccg
     2221 cacaaaaaag tataataagc acttgtatca tgatttaaag atacgcaaaa aataaaaaga
     2281 tttgaagttt agatttgtaa tgaaacgaag ttcgctaaaa aaaatagcgg aaatccggtt
     2341 agtcgtattt catttctttc attattttca tgaaagcatc tttgagcagc ggattaattt
     2401 cgacaggctt tttcgatgga acgggtttca aagtttcaat ggcatactcc atcagttttg
     2461 catcatcact caagttacaa aaagtgcaaa tttcatttat ggttttttga ggatgtgcag
     2521 caaggtcttc gtaccgaaca ggcaacacat ggttgggata tttcaaatct catagataca
     2581 atccactcaa caatagactt atcgagctcg cttgtaaact tttttatttc ctgtaaatga
     2641 ggagcaaggt caatatcttt cgatactatt tggtcgcaaa gcaaattcca ttttctatta
     2701 tttacacccc accagttgtg ggtttcttcg cctttttgtt cgccatgtat atcgctccac
     2761 cctgccgaag aaatggcagt atcccagcca ttgcgataca aaaatatggc tttaaaatcg
     2821 ggaaatattt tttttacaaa gtccatacga aaaacaagtt gaggatattt atcccacacc
     2881 cgttttgaaa aaactgtact cagataaaac ccaaaaatgt tgtgcatttt ttttatcgct
     2941 tcgggttgtg catccgattc atgcaattcg tacaaggcat tttcgcgtgt ataacttcct
     3001 attaaatctt cgtttttaaa taccgaatgc catagggctt taggctcgtt caaaaatgct
     3061 acatcgcggt gcatcgacat tactatgcct agtatagttg taccgctgcg gccggtgccc
     3121 aatatgaaag ttggttgttc tactttcttt tttattaacc ccaatttagc aaaactcata
     3181 ttggcaaata caatagggtt tatccattgc cctgcggtgg taaccggtcg tccttcgaaa
     3241 agagcatatg ataacatgcg cgaaaaaaac ttcgtccagc gggtacgtat atagttactg
     3301 ttaatttgtc ctaccataaa atgtttgttc agaatagact gtttatcact cgctggcaaa
     3361 tgtaataaag gcttacactt gtgccaatag ttagataaga tattgaaact tgacttacaa
     3421 agataaagta cggccaccat ccaccggaag gtttatacca ttgatatatc ctgcagccgg
     3481 tgaagctaaa aaagctacag cttcggcaat ttccacagct tcggcaaaac gtccagcagg
     3541 aatttcggcc agcatttcgc gggtaacatc attaaacgca cgtcctgttt ttttcgattt
     3601 atcttcaata atatttgtaa gtcgtgcagt attggtggct cccggcaaca cattgtttac
     3661 agtgatgcca taacgcgcta cttcgtgcga taatgttttg gcccaattgg caacggcagc
     3721 ccgtatggtg ttcgacacgc ccaagccttt tataggttgc tttaccgaag tagaaataat
     3781 attgatgatt cggccatagt ttgcagcctt cattccttcg aggcattcct gcaccagtaa
     3841 atgattgcaa ataaggtggt tggtaaaagc ggcagtaaat tcttctgcag tggcagtagc
     3901 gaccggcccg gccggtggac ctccggtatt gtttatcaaa atatgtattg catttttttc
     3961 gtcaatatat tgtgctactt tttgtttcaa ctcttgcgga aaagcaaaat cgccaactat
     4021 gtaatcgtgc tgttgccctg caaatgatgg caactctttt accacttctt tcaatacgtt
     4081 ttcgttgcgg gcaagtaaag ttacattgca ttccaattct gccaatgtaa ttgcaacagc
     4141 tttacctatt ccctgcgtgc tgccacatac caatgcacgc tttttttgta aatcaatatt
     4201 catatcattt tttttgaatg cgtaaaagta cataattgta cactgtatgt attataagtg
     4261 aaatactaac aacaaacaaa ttttgtcata tgt
//
LOCUS       DAORQA010000150         4291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5050_length_4291_cov_10.120869, whole genome shotgun sequence.
ACCESSION   DAORQA010000150 DAORQA010000000
VERSION     DAORQA010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5050_length_4291_cov_10.120869"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1583
                     /locus_tag="PK736_07205"
     CDS             204..1583
                     /locus_tag="PK736_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRC33216.1"
                     /translation="MMRKYFYPILLLSFVAFVSAIIVTQKKDKEKLTSPIKERRGQIT
                     MGSEWLNAKQAIEGLIASVDKNPNDTKAKMQLAMGYIQEARATGDHAYYDKAAMKLIK
                     DVLAEVNNNSNPSHKDMRFEATMAKSIIQASQHHFTDALNTANEAVKLNPYNAYVYGI
                     VCDANVELGNYTQAIDAADKMVSIRPDIKSYSRVAYLREIYGDFNGAKEAMQMAVNAG
                     YPGMEQTEWCRTQLAQLYEKTGVLDSANALYNMSLNFKPDYAYAHAGLGRIAQYNQKY
                     DQAIDYFKKANQAVVDYSFNQSICEIYMLQKNKTAADKFFDNALEQLQSGEHAANVED
                     DEMGHYADRELSNVYLLNGQYDKAIEHAIKEYNRRPANIDVNETLAWAYYKNAEYGKA
                     MQCINVAMKTNSQNAVLLKKASAIYAKNNLIERSKQLSMMAEKINPAIQKQLLCNPGF
                     NTSQLALKN"
     gene            1622..2275
                     /locus_tag="PK736_07210"
     CDS             1622..2275
                     /locus_tag="PK736_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HRC33217.1"
                     /translation="MKKATPFILLLFFTQIIIAQAHTINYQMEQQPTHNVVWYYLQLG
                     FQHILPDGFDHILFIISLCLVAKSAKQILWQCTAFTVAHTITLIMSMKNIIVAPPSII
                     EPIIALSIAFVAIENIFMKNLNAGRILLIFAFGLVHGCGFASSLNEIGLPRNQFYTSL
                     LSFNIGVEIGQLAVIGILFAAIIIWTRTKPWYSSYVVKPASIAIACISLFWVMERMA"
     gene            complement(2662..4098)
                     /locus_tag="PK736_07215"
     CDS             complement(2662..4098)
                     /locus_tag="PK736_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33218.1"
                     /translation="MKKYLVIIILFLCPNLIIAQCNNWSPFQLVKDTFPNPIFGNYLT
                     PECDKLSRPYVYVAAKDGGIKVYDISLPGNMILATTISTSLFGNVDAINIIQDSVYLY
                     VTLGDIWNTNEEAGLAIVDVSNPLSPVVLDYYLQPFATGGAAAVAIKNNYAYVAAMGN
                     GLIILDIGNKTNITFKSMLSLANNFPHTNTLGDTISKYNARGIALKDSLAYICYDRGG
                     LRIVNVSDVNNPVQTNQYCFDSLINKATAYNNIVIHNNLAFVTIDYYGLEILDITNPQ
                     NINQLSWWHPSGWAAATNDYLVWAASDGHANEIIFDSLCYRVYISAGRSDVVSISVVD
                     SLNPATCETYGSVTDTYGTWGLDYYDNNVYAAYIWSPFFPPYSNYTGLRQIQTTVCNS
                     SATNEIQQQNSLSIFPNPNNGEFYISPNKIFENGNLTVINPQGKKVFETKIIGNLFEN
                     VSVSLSPGIYFVMIRNCENVYTEKVIVY"
BASE COUNT         1419 a          770 c          806 g         1296 t
ORIGIN      
        1 cgcgtcagct gctgctgtat tccatatcaa aagccacatg cgctagccca atggattatt
       61 ggagaaaagt ccaacaatgg ctgtgctcaa agcctgatta atagtttgag acaactccaa
      121 ctatggcttt gaagaaaact ccaacatact taaatccaaa ccatcaacac taacgtatgt
      181 atttcaaaac cataaaaaat aaaatgatga gaaaatactt ttatccaata ttgttattaa
      241 gcttcgttgc atttgtgagt gcaattatag taacacaaaa aaaagacaag gagaaactta
      301 cttctcctat aaaagagcgc agagggcaaa ttacaatggg tagcgaatgg ctaaatgcaa
      361 aacaagctat cgaaggattg atagcatccg ttgataaaaa tccaaacgac acaaaagcaa
      421 aaatgcaact tgcaatgggt tatatacagg aagcacgtgc tacgggcgat catgcatatt
      481 acgacaaagc cgcaatgaaa cttattaaag atgtgctggc agaagtaaac aataacagca
      541 atccatcgca taaagatatg cgtttcgaag ccaccatggc aaaaagtatt atacaggcat
      601 cgcaacatca ttttaccgat gcacttaata ctgccaacga agctgtaaaa ttaaatccat
      661 acaatgccta cgtatacggt attgtttgcg atgcaaatgt agaattagga aattatactc
      721 aggcaattga tgctgccgat aaaatggttt ctattcggcc cgatataaaa tcatactcgc
      781 gtgtggctta cctgcgcgaa atttatggcg atttcaatgg cgccaaagaa gcaatgcaaa
      841 tggcggtgaa tgcaggctat cccggtatgg aacaaactga atggtgccgc acacaacttg
      901 ctcagctgta tgaaaaaacg ggggtacttg attctgcaaa tgcattgtat aatatgtcac
      961 ttaactttaa gcctgattat gcttatgcac atgcaggcct tggacgaata gcacaataca
     1021 atcaaaaata tgatcaggcc atagattatt ttaaaaaggc aaatcaagcg gtagtcgatt
     1081 attcttttaa ccaatcgata tgcgaaatat atatgttgca aaaaaacaaa actgctgccg
     1141 ataaattttt tgataatgca ttggaacaat tgcaaagtgg tgaacatgct gcaaatgtag
     1201 aagatgatga aatggggcat tatgccgaca gggaattatc gaatgtgtac ttactaaacg
     1261 ggcagtatga taaagcaata gaacatgcta taaaagaata caacaggagg ccggcaaata
     1321 ttgatgtaaa cgaaacgctt gcatgggcat attataaaaa cgcggagtac ggtaaagcaa
     1381 tgcagtgcat taatgtggca atgaaaacca attcgcaaaa tgcagtttta ttgaaaaagg
     1441 cttcggccat ttatgctaaa aataatttaa tagaacgcag taaacaattg agtatgatgg
     1501 cagagaaaat taatcctgca atacaaaaac aattgttgtg caatcccggt ttcaatacat
     1561 ctcaattggc actaaaaaat taatactctt agcaatgacc cctcattcat ttacgagcac
     1621 attgaaaaaa gccacgccat ttattttgtt gctctttttt acacaaatta taattgcaca
     1681 ggcacacacc atcaattatc agatggagca acaacctact cacaatgtag tgtggtatta
     1741 tttgcaactt ggttttcaac atattctacc ggatggtttc gatcatattt tgtttattat
     1801 aagtttatgt cttgtagcta aaagtgcaaa gcaaatattg tggcaatgca ccgcgtttac
     1861 tgttgcacat accatcacac ttattatgag catgaaaaat attattgttg ctccgccatc
     1921 tatcatcgaa cccatcattg cattatcaat agcatttgtt gccatcgaaa atatatttat
     1981 gaaaaacctc aatgcaggac gtatactttt aatttttgct tttggacttg tgcatggttg
     2041 cggatttgca agttcgctca acgaaattgg tttgccgcgc aatcaatttt acacttcgct
     2101 tttatctttc aacattggtg ttgaaattgg acaactggca gtgattggaa ttttatttgc
     2161 agcaataatt atttggactc gcaccaaacc gtggtacagt agttatgtgg taaagcctgc
     2221 ttccattgct attgcctgca tcagtttgtt ttgggtgatg gaaaggatgg cttaggttat
     2281 taagttattt ggtgattgag gacagtcatt tgttaatact tgtcattgga cattgttagg
     2341 atagttattt gttattgagt tatttggtta ttgactttgt catgctgagt gtcacagtga
     2401 gtattctcga actgcgaagc atctctgcgt attattggtc attgtcaccc tgagcgaagt
     2461 cgaaggggtt attgcgaatt gtcacagtga gtattgtcac cctgagcgaa gtcgagggga
     2521 ttctcgaact gcgaagcaat ctttgccagt catttgtcat gtgtgcgcaa gccattgttt
     2581 gtgtcctcac aaacaattcc caaattatta ttggtcattt tcgaaattct agttactata
     2641 aatattattt atacaaagcc attaataaac gatcactttt tctgtataaa cattttcgca
     2701 atttcttatc atcacaaaat atattcccgg tgataaacta accgaaacat tttcaaaaag
     2761 gttgccaata atttttgttt caaaaacttt ttttccttgc gggtttataa ctgttagatt
     2821 tccgttttcg aaaattttgt ttggcgaaat ataaaattct ccattgttag gattaggaaa
     2881 tatagacaat gaattttgtt gctgtatttc atttgttgct gatgagttac acaccgtagt
     2941 ttgaatctga cgtaagccgg tatagtttga ataaggagga aaaaatggcg accaaatgta
     3001 tgcggcatat acattgttgt cataataatc taaaccccat gttccgtaag tatcggtaac
     3061 agatccatat gtttcgcagg ttgccgggtt taacgagtca actaccgata ttgaaacaac
     3121 atcgctgcgc cctgcagaaa tatacacacg gtaacaaagc gagtcgaaaa taatttcatt
     3181 tgcatgacca tcggaggcgg cccaaacgag ataatcattt gtggccgctg cccaacctga
     3241 aggatgccac caacttagtt gattaatatt ttgcggattg gtgatatcta aaatttcaag
     3301 accgtaatag tctatagtaa caaaagcaag attattgtgg attacaatat tgttgtatgc
     3361 tgttgctttg ttaattaacg aatcgaaaca atattgattt gtttgcacag gattatttac
     3421 atccgataca tttacaatac gaaggccacc acggtcgtaa caaatataag ccaacgaatc
     3481 tttcaaggcg atgccacgtg cattgtattt acttatagta tcgccaagtg tattggtatg
     3541 tggaaaatta ttggccaggg aaagcattga tttgaatgta atatttgttt tgttgccaat
     3601 gtcaagaata attaatccat tgcccattgc tgcaacataa gcgtaattgt tttttatcgc
     3661 caccgctgca gcaccaccag ttgcaaatgg ctgcaaataa taatctaaca ccactggcga
     3721 taatggattg ctcacatcaa caattgccaa cccggcctct tcgtttgtat tccatatatc
     3781 gccaagtgtt acgtataagt aaacgctatc ctgaattata tttatagcat ccacattgcc
     3841 aaacaaagat gtggatatag ttgttgccaa aatcatattg cccggcaacg atatatcgta
     3901 cacttttatg ccaccatctt ttgcagctac ataaacataa ggcctcgata gcttatcgca
     3961 ttcgggtgtg aggtagttgc caaaaatggg attaggaaaa gtgtctttca ccagctgaaa
     4021 aggtgaccag ttattacatt gtgcaattat aagattgggg cacaaaaata aaatgataat
     4081 gaccaaatat tttttcatag ccaagagttt tagtaatgtg aaatcgacag tggtaaatat
     4141 aataattttt aacagaaata ttttcccttt tttaatccat aaaaaaaagc attttcaaac
     4201 catttcaaat taccaatgaa attttgattt taaactgatt acgaaaaaaa aggacctttc
     4261 aaaataatgc cagtttaaga tatgtatata t
//
LOCUS       DAORQA010000151         4233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5127_length_4233_cov_21.122547, whole genome shotgun sequence.
ACCESSION   DAORQA010000151 DAORQA010000000
VERSION     DAORQA010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5127_length_4233_cov_21.122547"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /gene="neuB"
                     /locus_tag="PK736_07220"
     CDS             complement(<1..946)
                     /gene="neuB"
                     /locus_tag="PK736_07220"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004276711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HRC33219.1"
                     /translation="MPQQKKCIIIAEAGVNHNGDINMAKQLVDAAANAGADFVKFQTF
                     VATNAVTANAKKAQYQITNTGNNSSQLDMIKKLELNHEQHFELIKYCKLKKIKFLSTA
                     FDLESVAFLKSLKLDLIKIPLGEITNLRLLEACGGQNKNILLSTGMCNLAEINDAIKV
                     IVKSGTSKNKITLLHCTTEYPAPFKDINLNAMKTMQQKFKLPVGYSDHTEGIAISLAA
                     VAMGATVIEKHITTDRTLPGPDHRASIEPDEFAQLVKGIREIELSMGDGEKKAMPSEK
                     KNIAIARKSICAKTDIKKGTLLNDSLLTAKRPGTGISPM"
     gene            complement(1032..>4233)
                     /locus_tag="PK736_07225"
     CDS             complement(1032..>4233)
                     /locus_tag="PK736_07225"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC33220.1"
                     /translation="NDNNSWYVYAALLGENKIAAIKLTDSVNASAQLLLNNSFPGMNS
                     PLGFAMIRDSSNWHGIAGAGGTLNHFVFANNCNATPPQSNYFAGSANVNAPLGSYQRI
                     AIQALDTNYITHSFIDSVYFDTTLPEVSFTVNNFCTGLLAQFQSIELLCFDTLIQRYW
                     NFGDNTFDSIISNPTHIYSNAGTYTVTFTINTTQGNTITSQQIITVKDAPVADFTFSD
                     NQCADLNVLFTNTSTASVNDTIIQNRWSVENVLVAQTKDFNFTFDSSGLYIIKLVVTN
                     SNGCDDSLIKQITILDSPTPSFTVTNSCIGGTVNFLNATDSNNLNVNYIWNFGDTTSS
                     TDVNPVHQYTNGLLNHNVQLISIGSNGCSDTLLQNIHLSVKPTPVILNTSNVLCQNQE
                     IVFANNSFANGVDLISSIIWDFGDSTFSNIADTVKHKYNLPGTYTVTLTAATPTFCDS
                     SVTYTVKVNASPKASYTAPDICFGDTTYFTNTSTVASPDTIQYFAWYLVDIDSSISIP
                     NFSYYYQTGSHPVQLIVVSDSGCVDTLNKDVKVYGVPDVTFSKSNIACTDNATIFNNT
                     TSLIVVDNDSIALWNWNFGDNQFSGLESPIHTYTDTLEYNVSLKATSLFGCKDSLTVA
                     VKVNKSPEPYFTFSPACQKQLTFFQAKDSSATQFPPTTYSWNFGLPNANSTFPNVPYY
                     YDTAKIYLVALTLTYNNGCKQKYLQPVLVNRKPYADFISSGNCSGQSLLALNQSNFYN
                     DSVASYTWSVNGNNISNDTNLTYIPNQSGNYALQLIAISNKTCSDTIVKNVNVNPTPN
                     AAFTTIPTIGAFNSPITFSNQTTGAVAYNWSFGDNTFDNTFEPNHTYTTTNNFYTQLI
                     AISDSNCTDTAFQYIKIVESFIDLAVNNVEFKKSNQQLSFSAIVKNNSNIDIQYFTIT
                     ARIEGLPDIIDNPQIVLQANNGFTKYSFVSSVLLNGNFNPRYVCIEVTKPNDLADQVK
                     ANDNTCVSITGGFEITLLNTDDGSIEMAINSDIKDECTYEIFDIQGKIISTNNLVLSK
                     GLNYVQLPFHHTNGQYMVRCINKAGDTVSRRLFFAK"
BASE COUNT         1220 a          741 c          865 g         1407 t
ORIGIN      
        1 gcatgggtga aatgcctgtg ccgggtcgtt ttgcagtgag caaactgtcg ttaagcaatg
       61 ttcctttttt gatatcggtt tttgcacaaa tactttttcg tgctatggca atattttttt
      121 tctccgatgg catggctttt ttttcaccat cgcccatcga taattctatt tcacgaattc
      181 cttttacaag ttgtgcaaat tcatcaggct caatcgaggc acgatgatcg ggcccgggta
      241 aagtcctgtc ggttgtgata tgtttttcga tgacggtagc gcccatggcc accgctgcaa
      301 ggcttatggc aataccttca gtatgatccg aatatccaac cggcaattta aatttttgtt
      361 gcatagtctt cattgcattc agattaatat ctttaaatgg tgccggatat tcagtggtgc
      421 aatgcagcaa tgttatttta tttttcgatg ttccgctctt tacaatcact ttgatggcat
      481 cgtttatttc tgccagatta cacatgccgg tagagagcaa aatatttttg ttttgaccac
      541 cgcaggcttc aagcaagcgc aggtttgtaa tttcgcccaa aggtattttt attaaatcca
      601 atttaagcga ttttaaaaac gccacacttt ctaaatcgaa ggccgttgaa aggaatttta
      661 tcttttttag tttgcaatat tttatcagct cgaaatgttg ctcgtgattt aactcaagct
      721 tttttatcat gtcgagctgc gatgaattgt tgccggtatt ggtgatttgg tattgtgctt
      781 tcttagcatt ggctgtaact gcattggtgg ccacgaaagt ttgaaatttt acgaaatcgg
      841 caccggcatt tgcagcagca tcaaccaatt gctttgccat gtttatatcg ccattatggt
      901 ttactccggc ttcggctatg atgatacatt ttttttgttg aggcatactg tgtgataatt
      961 cgctgtggcc tcaaatgtag aaaaatgatt ggcacacatg agaagaattt attccttttc
     1021 ggagtataaa tttattttgc aaaaaacagc ctgcgcgaaa cggtgtcgcc agctttgttt
     1081 atacacctta ccatatattg gccattggta tgatgaaagg gcagttgaac ataatttaac
     1141 cctttgctaa gcacaagatt gtttgttgaa attatttttc cctgtatatc gaaaatttca
     1201 taagtgcatt catcttttat atccgaattt attgccatct caatacttcc atcatcggta
     1261 ttaagcaaag taatttcgaa accacctgta atgctcacac atgtattgtc atttgccttt
     1321 acctgatctg ctaaatcatt tggcttggta acttctatgc aaacatagcg gggattaaaa
     1381 ttcccattga gtagtaccga tgacacaaaa ctgtattttg taaatccatt atttgcttgc
     1441 aaaacaattt gtggattatc gattatgtca ggcaaacctt caattcgtgc ggttatagta
     1501 aaatattgaa tatcgatatt gctgttgttt ttcactatgg ccgagaatga taattgttga
     1561 ttcgattttt taaattctac attatttaca gccaaatcga taaacgactc tacaattttt
     1621 atgtattgga aagcagtgtc ggtgcaattg gaatcggata ttgctatcag ttgagtatag
     1681 aaattatttg ttgtagtata agtatgattt ggttcgaagg tgttatcgaa agtgttgtca
     1741 ccaaaactcc agttatatgc aacagccccc gtagtttgat tcgaaaaagt gataggtgaa
     1801 ttaaatgcgc ctattgttgg tatggttgta aatgctgcat ttggagtagg atttacgttt
     1861 acgtttttca ctatggtatc gctgcatgtt ttatttgata ttgcaataag ttgcaatgca
     1921 taattgcccg attggttagg aatataagta agattggtat cgttcgaaat gttgtttcca
     1981 ttcacactcc atgtgtatga agcaacagaa tcattataaa agttggactg atttagagca
     2041 agtaaacttt ggcccgaaca atttcctgaa gaaataaaat cagcataagg cttgcggtta
     2101 accaatacag gttgcagata tttttgttta caaccgttat tgtatgtgag ggtaagtgct
     2161 acaaggtata tttttgcagt atcataatag tatggtacat tggggaaagt gctattggca
     2221 tttggcaaac caaaattcca cgagtaagtt gttggcggaa attgagtagc agaagaatct
     2281 tttgcctgaa agaaagtcaa ttgcttttgg caagctggcg aaaatgtgaa gtacggttcg
     2341 ggcgatttgt tcaccttaac agcaaccgtt aatgaatctt tgcaaccaaa aagtgatgtt
     2401 gcctttagcg aaacattgta ttccaaagta tcggtgtagg tgtgaatggg tgattctaaa
     2461 ccggagaatt gattgtcgcc aaaattccaa ttccacaatg cgatagaatc attgtcgaca
     2521 acaatgagtg aagttgtgtt gttaaatatg gtagcattgt cggtacacgc gatattgctt
     2581 ttagaaaaag ttacatcagg tacaccatac actttcacat ctttatttaa tgtatccaca
     2641 catcccgaat cgctaactac tatcagttga accgggtgac tacctgtttg gtaatagtat
     2701 gaaaagttgg gaatgcttat ggatgaatct atgtcaacaa gataccatgc aaaatattgt
     2761 atagtgtcgg gcgatgctac tgtacttgtg ttggtgaagt atgttgtgtc accaaaacaa
     2821 atgtcagggg cagtgtatga agcttttggc gatgcattaa cctttactgt atacgttacc
     2881 gatgaatcgc aaaatgttgg ggtggccgca gtgagtgtta cagtataagt gccgggcaga
     2941 ttgtatttat gctttacagt atcggcaatg ttcgaaaatg tactatcgcc aaaatcccaa
     3001 atgatcgagc ttattaaatc cacaccattg gcaaaagaat tatttgcaaa aactatttcc
     3061 tgattctgac aaagtacgtt gcttgtgttt agtattacag gagtgggttt taccgataaa
     3121 tgtatatttt gcaaaagggt atcgctgcag ccattgctgc ctatgcttat taattgcaca
     3181 ttatgattga gtaaaccatt ggtgtattga tgcacaggat ttacatcggt gcttgacgta
     3241 gtatcaccaa aattccaaat ataatttaca ttgaggttat tgctgtcggt tgcatttaaa
     3301 aaattcacag tgccaccaat acaactattg gttacggtga aagatggagt aggggaatct
     3361 aatattgtga tctgtttaat taaactatca tcgcatccat tcgaattcgt tacaaccaac
     3421 ttaataatgt acaaaccact gctgtcaaaa gtaaaattga aatcttttgt ttgtgctacc
     3481 aaaacatttt ctacgctcca tcgattttga atgatggtat cgtttacaga tgcagtgctt
     3541 gtattggtga acaacacatt taaatcggca cattgattgt cggaaaaagt aaaatcggca
     3601 acaggggcat ccttcaccgt tattatttgc tgactggtaa tggtattgcc ctgagtagta
     3661 tttattgtaa acgttactgt atacgtaccg gcattgctat agatgtgggt gggattgctg
     3721 ataatagaat cgaatgtgtt gtcaccaaaa ttccaatacc gttgtatcaa agtatcaaag
     3781 cacaataatt ctatggattg aaattgtgca agcaaccctg tgcaaaaatt attaacggta
     3841 aatgacactt caggcaaagt agtgtcgaag tacacactat ctataaatga atgggtaata
     3901 tagtttgtgt ccaaagcctg aatagcaatt cgttgatagc tgcccaacgg agcatttaca
     3961 tttgcagatc cggcaaagta attactctgt ggcggtgtgg cattgcaatt attcgcaaac
     4021 acaaaatgat ttaatgttcc gcctgcacct gcaatgccat gccagttact gctgtcgcgt
     4081 atcattgcga accccaatgg gctattcatt ccgggaaacg aattgtttaa caacaattgc
     4141 gctgatgcgt ttacactatc tgtaagtttg attgcggcaa ttttattttc gccaagcaat
     4201 gctgcatata cataccacga gttattatca ttt
//
LOCUS       DAORQA010000152         4190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5191_length_4190_cov_11.244256, whole genome shotgun sequence.
ACCESSION   DAORQA010000152 DAORQA010000000
VERSION     DAORQA010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5191_length_4190_cov_11.244256"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PK736_07230"
     CDS             <1..211
                     /locus_tag="PK736_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33221.1"
                     /translation="VLAQGHIIKYCIEIKLGKTPSVSKGFYQSLADLKCKNAFVICNT
                     SHPYKTREGVMFTDLKSFIKLHLIK"
     gene            complement(299..1378)
                     /locus_tag="PK736_07235"
     CDS             complement(299..1378)
                     /locus_tag="PK736_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRC33222.1"
                     /translation="MIKFNINQDISQAETMPANFYTDENVFSQTKEKIFSSSWQLVEC
                     GAGVFSEDEAYPFMLLPGFMNEPLVLINNNDNQLKCMSNVCTHRGNILIEQPGKSKSI
                     VCAYHGRKFNNSGRFVSMPEFEEVQNFPRACDHLHQVKLQSWNQFHFVGLNCNHDLKS
                     FFTQLEMRLGFMNVSQFRYAPELNREYLVHANWALYCDNYLEGFHVPFVHQGLNKVLD
                     YKNYTTEIFDNFNLQIGIADNDADCFHFPESHIDKGKKIAAYYYWLFPNTMFNFYPWG
                     LSVNIVKPLSVNKCKVSFITYVYDETKMQTGAGANLDKVEREDEAVVEAVQRGMQSRF
                     YNTGRYSPTREKGVHHFHSLIAKSF"
     gene            complement(1498..1962)
                     /locus_tag="PK736_07240"
     CDS             complement(1498..1962)
                     /locus_tag="PK736_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRC33223.1"
                     /translation="MPNINLETKIAAPINICFDCARNIDLHQQSVVHTKEIAIAGRTS
                     GLIEEGETVTWQAVHFGVKQTLTVKIEAMVYPTYFCDVMIHGAFKSMRHEHFFVEQNN
                     FCVMTDKFSYTTPLGIFGKIFDKIFLENYLRKFLLTRNNFIKAEAEKQFRQI"
     gene            complement(2028..4001)
                     /gene="gyrB"
                     /locus_tag="PK736_07245"
     CDS             complement(2028..4001)
                     /gene="gyrB"
                     /locus_tag="PK736_07245"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRC33224.1"
                     /translation="MEVNDTVEEKKPQNQSYDADSIQVLEGLEAVRKRPAMYIGDIGI
                     KGLHHLVYEVVDNSIDEAMAGHCKNINVFINENNSITVIDDGRGIPTDMHAKEKKSAL
                     EVVMTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSSHLKVEVYRNGKKYVQEYSCGK
                     PLYSVKEEGETTSRGTTVTFLPDSSIFITDVYQYDTIAARMRELSYLNKGIRITLTDK
                     REKDENGNHATEEFYSTRGLIEFVEFLDATREKLTEAPIYIENLKGIIPVEVALQYNT
                     SFNENLHSYVNNINTHEGGTHLAGFRRALTRTLKAYADKSGLLDKLKIEITGDDFREG
                     LTAVISVKVQEPQFEGQTKTKLGNNEAIGAVDTAVGDALKDYLEEHPRDAKNIVSKVV
                     LAAQARYAAKKAREMVQRKNILTGGGLPGKLSDCSDSDPMRCEIFLVEGDSAGGTAKQ
                     GRNRSYQAIMPLRGKILNVEKAMQHKIFDNEEIRNIFTALGVSIGTADDAKELNLDKL
                     RYHKVVIMTDADVDGSHISTLILTFFFRYMKELIENGYVYIASPPLYLVKKGKEERYC
                     WNEVQRKQAVLEIGGEGKESTVGVQRYKGLGEMNAEQLWMTTMNPELRTLKQVTIDSA
                     AEADRIFSMLMGDEVPPRREFIEKNAKYANIDA"
BASE COUNT         1214 a          847 c          709 g         1420 t
ORIGIN      
        1 ggtgctggcg caaggccaca taataaaata ttgtatagaa attaaattag gaaaaacacc
       61 ttctgtgtcg aaagggtttt atcaatcgct cgcagatcta aaatgcaaaa atgcatttgt
      121 gatatgcaat acatcacatc catacaaaac ccgtgaaggg gttatgttta ctgatttaaa
      181 gtcatttatt aaattgcatt taattaagta accaaccttg aaaatccact ttcaaatagt
      241 ggattttcaa tgttgtgaaa ttttataaat accctcggtt aaaccaaaaa tatccatttc
      301 aaaaactttt tgcaataaga gaatgaaaat gatgtacacc tttttcgcgg gtgggtgaat
      361 accttccagt attatagaaa cgtgattgca taccacgttg cactgcttct actacagcct
      421 cgtcttcgcg ctctacttta tcgagattgg cacctgcgcc tgtttgcatt tttgtttcat
      481 cgtaaacgta ggttataaac gacactttgc atttgttcac cgataaaggt tttacaatat
      541 ttacacttaa accccagggg taaaaattga acattgtgtt tggaaacaac caataataat
      601 aagcagcaat tttttttccc ttatcgatat gactttcagg aaagtgaaaa caatccgcat
      661 cgttgtcagc aatgccaatt tgcaaattaa agttatcaaa aatttcggtg gtgtaatttt
      721 tatagtcgag caccttattc aatccttgat gtacaaaagg aacatgaaat ccttcgaggt
      781 aattatcgca atataaggcc cagtttgcat gtaccagata ttcacgatta agctcaggtg
      841 cgtatcgaaa ttgcgaaaca ttcataaaac ccaaacgcat ttccaattga gtaaaaaacg
      901 attttaaatc atggttacaa tttaatccca caaaatgaaa ttgattccac gattgcaact
      961 ttacctgatg caggtgatca catgcgcggg ggaaattttg cacctcttca aattcgggca
     1021 tactcacaaa acgtccgcta ttattgaatt tgcggccatg gtaggcgcac actatgcttt
     1081 tactttttcc aggctgttct atcagaatat ttccacggtg tgtgcataca ttgctcatgc
     1141 atttcagttg gttgtcattg ttattgatga gcactaatgg ctcgttcata aaaccgggaa
     1201 gcaacataaa agggtaagcc tcatcttcgc taaatactcc ggcaccgcac tcaaccaatt
     1261 gccacgatga ggaaaatatt ttttctttcg tttgtgaaaa aacattttcg tcagtgtaaa
     1321 aatttgccgg catcgtttcg gcttgagata tatcttgatt aatgttgaat tttatcattg
     1381 cacaaaaata taaaagtaag gttcagtgca taaagaaata ttttattgta gcaaaaagta
     1441 ttctcacgta atacttcaaa aaaactttgc atttaatgaa gtatattttc ggtgttatca
     1501 aatttgtcga aattgttttt ctgcttcagc ttttataaaa ttatttcgtg tcaacaaaaa
     1561 ttttcgcaag tagttctcaa gaaaaatctt atcgaatatt tttccaaaaa tgcctagtgg
     1621 tgtggtgtag gaaaatttat ctgtcattac acaaaagttg ttttgttcaa caaaaaaatg
     1681 ctcgtgcctc atcgatttaa atgcgccatg tatcatcaca tcgcaaaagt aagttggata
     1741 aaccatcgct tcaattttta cagtgagtgt ttgtttcact ccaaagtgaa cagcctgcca
     1801 tgtaacggtt tcaccttctt ctatcaggcc agaagttcga ccggcaattg caatttcttt
     1861 tgtatgcacc actgattgct gatgcaaatc aatattacgt gcacaatcaa aacatatgtt
     1921 tataggtgct gcaattttgg tttctaaatt aatatttggc atgatgtaaa aataaaaaag
     1981 gcatactcaa atgaatatgc cttttcaaaa tattttatct aatttaatta tgcatctatg
     2041 tttgcatact tagcattctt ttcaataaac tctctgcgtg gcggtacttc atcgcccata
     2101 agcatgctga aaattcggtc ggcttcggca gcactatcaa tagttacttg tttcaatgtg
     2161 cgcaattcag gattcattgt tgtcatccac aattgttcgg cattcatttc tcccaaacct
     2221 ttgtatcgct gcacacctac agtactttct tttccttcgc cacctatttc gagtaccgcc
     2281 tgcttacgtt gcacttcgtt ccagcaatag cgttcttctt ttcccttttt taccaggtat
     2341 agtggtggcg atgcaatgta aacgtatccg ttttcgataa gttctttcat ataccggaaa
     2401 aagaaagtaa gtatcaaagt actgatgtga ctaccgtcaa catcggcatc ggtcatgatt
     2461 acaactttat ggtaacgcaa tttgtcgagg ttaagttctt tggcatcatc agcggtacca
     2521 atgcttacgc ccagtgcagt aaagatgttt cgtatttctt cgttatcgaa aattttatgt
     2581 tgcatggcct tttccacatt gagaatttta ccccgcaatg gcataatggc ttgataactt
     2641 ctattacgcc cttgtttggc agtaccaccg gcactatcgc cctctactaa aaatatttcg
     2701 caacgcatag ggtcactatc actacaatcg ctaagcttgc cgggcaatcc gccaccggta
     2761 agaatatttt tacgctgcac catttcgcgc gccttttttg ccgcgtatcg tgcctgtgct
     2821 gcaagtacaa ctttgctcac aatatttttt gcatcgcgtg gatgttcttc caaataatct
     2881 ttcaaagcat cgccaactgc agtatccacc gcacctatgg cctcgttgtt accaagtttg
     2941 gtttttgttt gcccttcaaa ttgtggctct tgcactttta cagaaataac tgctgtaagt
     3001 ccttcgcgaa aatcatcgcc cgtaatttct attttcaatt tatcgagcaa tcccgatttg
     3061 tcggcatacg cttttaatgt gcgtgtaagt gctctgcgaa atcctgcaag gtgggtgccg
     3121 ccttcgtggg tgtttatatt gtttacatat gaatgcaaat tctcgttaaa cgatgtattg
     3181 tattgcaagg caacttctac aggaataatt ccttttaggt tttcgatata aataggagct
     3241 tctgttaact tttcgcgcgt agcatccaaa aattcgacaa actctattaa gcctcttgtg
     3301 ctataaaatt cttcggtagc atggtttcca ttttcatctt tctctcgctt atcggttagc
     3361 gtaattcgaa tgcctttgtt caagtatgat aattcgcgca tacgggcagc aatggtatca
     3421 tattgataaa catcggtaat aaaaatcgaa ctatcgggca aaaaagtaac agtagtgcct
     3481 cgtgatgttg tttctccttc ttcttttaca ctatataaag gcttaccaca tgagtattcc
     3541 tgtacatatt ttttgccatt gcgatacact tcaactttta agtggctcga taaagcattt
     3601 acgcaagata cacctacacc atgcaatcca cctgatactt tatatgagtc tttgtcgaat
     3661 tttcctccgg catgcaatac tgtcattact acttcgagtg cgcttttctt ttccttggca
     3721 tgcatatccg ttggaatacc gcggccatca tctattacag taatagaatt gttttcatta
     3781 ataaatacat tgatgttttt gcaatgccct gccatggcct catctatgga gttatccact
     3841 acttcgtata ccaaatgatg cagacctttt atgccaatat caccaatata cattgcaggt
     3901 cgcttccgca ctgcttcgag accttccaat acctgaatac tgtcggcatc ataactttga
     3961 ttttgtggtt ttttttcttc cacggtgtcg ttcacttcca ttataatttt tgaatattaa
     4021 tttaaattga taaataaatt tcggggtttt ttcatttgtt tcaaaagtac tgtattttca
     4081 gctatttcac aatatttttt aaataaaaat aacagaatat ttactaacaa attgcaaata
     4141 attttttaca ggtttttatt gcaaaatcga atagtattac agttataaat
//
LOCUS       DAORQA010000153         4174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5231_length_4174_cov_6.871085, whole genome shotgun sequence.
ACCESSION   DAORQA010000153 DAORQA010000000
VERSION     DAORQA010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5231_length_4174_cov_6.871085"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>970
                     /locus_tag="PK736_07250"
     CDS             <1..>970
                     /locus_tag="PK736_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33225.1"
                     /translation="VYADAKSKGVHQFEIQLQTLSGEVSDANNKRIVFIDIIEAKEKV
                     LILADSPHPDLGAMKNAIEVSRNYDAKIEYANSFTGNFADYELVILHQLPSFDNKMEA
                     LSEKLKTADVSVMYVLGAQTDANTFNKMQGVLNIGSANGKTNATQANFNNSFSLFTVS
                     DNDWKYIQNLPPLTSPFGVYKSTANNYVMYRQRLGNISTDQPLFLFNQTGEKKIAVLA
                     GEGIWRWRIKEFEERSTQLITDDFLQKAVQYLCVKDKREPFKVKWKNNYYENEPIKME
                     AELYNKSGELINAPDIKLSVFNSDGKAFDYNFSKTEKAYSISLGNYP"
     assembly_gap    971..980
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <981..1306
                     /locus_tag="PK736_07255"
     CDS             <981..1306
                     /locus_tag="PK736_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33226.1"
                     /translation="FKALAKNGEEQLSKTGSFTVSPLQVEAIETIADHQLMNLIAEKS
                     GGKMIYPQQLETLKDIIKARKEITSVSYEQKHLKDLINLKWVFFLLFLFLAIEWFLRK
                     RVGTY"
     gene            1319..2548
                     /locus_tag="PK736_07260"
     CDS             1319..2548
                     /locus_tag="PK736_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRC33227.1"
                     /translation="MLTILVSIVVISFLFERWLDYLNSKTWTTSLPFELQGLYDEEKY
                     AKAMQYDKVNNRFENFTATLSFTATLIMLLMGVFGKWYNYCESFGLSYTLTHFLFFGS
                     LFIFSDLLNLPFQLYQTFNIEQKFGFNQTTLSTFFADKIKGYLLGILIGGPLMAAFIY
                     FYQYSGPNFWIYAWVVISGFSLLMAAFYSSLILPLFNKLKPLEHGDLRSAIENYCNKT
                     DFKISNLMVMDGSKRSAKANAFFTGLGQSKKIVLFDTLINQHTQEELVAVLAHEVGHY
                     KHRHILVSMIISVLQMGVLLYIFSLFVNEPAISVAMGGNKPVLALGILGFGILYSPLS
                     LITSLVMNFISRKNEYEADAFAAKTYSAEPLMNALKKLSVNHLSNLTPHAAYVFFNYS
                     HPTLLQRIRALNKLSKQ"
     gene            complement(2572..3573)
                     /locus_tag="PK736_07265"
     CDS             complement(2572..3573)
                     /locus_tag="PK736_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC33228.1"
                     /translation="MLPAPKILIIGQGIAGTVVSFMLSQQQIEHDVIDNSNPSSSSQI
                     AAGIVHPITGRRLAIAPQYNVALHEAKLFYQEMSKQLGNTFFEQKNILEIYADVKHRN
                     DWLARSADDDCAKLQCSEIQQSSLQNISAPQGAMCIAMGHFLKIKLLLDSYKNVLASE
                     NKIQTEKFIYSALQIQDENILYNDVKYSHIIFCEGSAASLNPFFPALPFHLCKGEILD
                     AYIPELTEEYIINKNIYILPMGNQIFKIGSNFEWDFKDDKPTELIRQQLESSLQTIIK
                     CNYEIINHQAAIRPTVKNREPICMWHTQYKNIGILNGLGTKGALLAPLLAKKLLVDF"
     gene            complement(3576..>4174)
                     /locus_tag="PK736_07270"
     CDS             complement(3576..>4174)
                     /locus_tag="PK736_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33229.1"
                     /translation="LLLNKNINIYKLKKIIVAHCVLGIVAIVVFLFFKNEKNASISQT
                     INILFICSGVALCGIALKSKLHFLIRMYFSIYALSFLLFATNTSALVGFIANFDFYSN
                     AENKFNIRDNYFIELQTSMLTNESLQPRYKMYQRIFYFNKTIVRDIDFEGKLDSVIFI
                     GMANDSLRVAGYSQNKIVEKMIPTKPVSSKTTIEQKRN"
BASE COUNT         1309 a          694 c          797 g         1364 t
ORIGIN      
        1 gtatacgctg atgcaaaaag caaaggtgtg catcaatttg aaattcaact tcaaaccctt
       61 tcgggggagg tgagcgatgc aaataataag cgtattgttt ttatcgatat aatagaagca
      121 aaagagaaag tactcatatt ggccgattcg ccacaccccg atttgggagc aatgaaaaat
      181 gccattgagg ttagccgtaa ctatgatgct aaaatagaat atgctaattc atttacaggc
      241 aactttgccg attatgagtt ggttatattg catcaactac cttcgttcga taataaaatg
      301 gaagcccttt ccgaaaaatt gaaaactgct gatgtttcgg tgatgtatgt tttaggagca
      361 caaaccgatg caaatacatt taataaaatg cagggagtac ttaatatagg ttcggcaaat
      421 ggaaaaacga atgcaacgca ggcaaacttc aacaattcgt tttcgctctt tactgttagc
      481 gacaacgact ggaagtacat acagaattta ccgccactca caagtccttt tggtgtttac
      541 aaatcaacgg caaacaacta tgtaatgtat cgtcagcggt tgggaaatat tagcactgat
      601 caaccattat ttttatttaa tcaaacaggc gaaaagaaaa ttgctgtatt ggctggcgaa
      661 ggaatttggc gttggagaat aaaggaattt gaagagcgta gcacacaact gattactgat
      721 gactttttac aaaaggccgt acaatattta tgcgtgaagg ataagcgtga accatttaaa
      781 gtaaaatgga aaaacaatta ttacgaaaac gaaccaataa aaatggaagc tgaattgtac
      841 aacaagtcgg gagaattgat taatgcaccg gatataaaat tatcggtatt taacagtgat
      901 ggcaaggcat tcgattacaa tttcagcaaa accgaaaaag catattccat ttcgttgggc
      961 aattatcccg nnnnnnnnnn cttttaaggc tttggcaaag aatggtgaag aacaattgtc
     1021 aaaaacagga agttttacag tttcaccatt acaggtagaa gcaatagaaa caatagccga
     1081 ccaccaactc atgaatctta ttgcggaaaa aagcggaggt aaaatgattt atccgcagca
     1141 attagaaaca ttgaaagata ttatcaaagc acgaaaagaa ataacatcgg tatcctatga
     1201 gcaaaagcat ctcaaagact taataaattt aaaatgggta ttttttcttt tgtttttatt
     1261 tctcgccata gaatggtttt tgcgtaagcg tgttggtacc tattaatttt ttattttcat
     1321 gttaaccatt cttgtttcta ttgttgttat aagtttttta tttgagcgat ggctggatta
     1381 tcttaatagt aaaacatgga caacatcatt gccatttgaa ttgcaggggc tttatgatga
     1441 agagaagtat gcaaaggcaa tgcaatacga caaagtaaat aatcggttcg aaaattttac
     1501 tgcaacacta agttttactg ctacacttat catgctgctg atgggcgttt ttggaaaatg
     1561 gtacaattac tgcgaaagtt ttggccttag ttacacctta acgcatttct tattttttgg
     1621 ttcgcttttt attttttccg acttattgaa tttgccattt caactctacc aaacatttaa
     1681 tatcgaacag aaatttggat ttaatcaaac aacactttct actttttttg cagataagat
     1741 taaaggatac ttgctcggaa tattgatagg tggcccgtta atggcagcct tcatttattt
     1801 ttatcaatat tcggggccaa atttttggat atatgcgtgg gtggtgatca gcggatttag
     1861 tttgcttatg gcggcctttt attcatcatt aattttgccg ctgttcaata aattgaaacc
     1921 gctggagcat ggagatttgc gctcggccat tgaaaactat tgcaacaaaa ccgattttaa
     1981 aatcagcaac ttgatggtga tggatggaag caaacgcagt gcaaaggcca atgctttttt
     2041 taccggttta ggtcaatcga aaaaaatagt attgttcgat acgttaatca atcagcatac
     2101 tcaggaagaa ttggtggctg tgttggctca tgaagtggga cattataagc atcgtcatat
     2161 tcttgtttct atgattattt cggttttgca aatgggtgtg ttgctttata tattttcgtt
     2221 gtttgtaaat gagcctgcca tatccgtagc aatgggaggc aacaagcctg tgttagcttt
     2281 gggcatttta ggatttggaa ttttatattc tccattatcg ctgattacat cattggtcat
     2341 gaattttatt tcacgcaaaa acgaatatga agcagatgct tttgccgcca aaacgtattc
     2401 ggccgaacct ttgatgaacg cattgaaaaa actatctgta aatcatttaa gcaatctcac
     2461 accgcatgca gcgtatgtgt tttttaatta ttcgcaccca actttgttgc aaaggataag
     2521 ggcattgaat aaattatcaa agcaatagtt tgtcttaact ttttcaactt tttaaaaatc
     2581 gaccaacaat ttttttgcca gcaaaggtgc caacaaagcg cctttcgttc ctagcccgtt
     2641 tagtatgccg atgtttttat attgtgtgtg ccacatacaa atgggctcac gattttttac
     2701 cgtaggacga attgccgcct ggtgattaat gatttcgtag ttgcatttga ttatagtttg
     2761 caacgatgat tctaactgct gccttattaa ttcagttggc ttatcatctt taaaatccca
     2821 ttcgaaattc gaaccaatct taaaaatttg atttcccatt ggcaaaatat aaatgttttt
     2881 attgatgata tattcctctg ttagttctgg aatataagca tcgagaattt cgcctttaca
     2941 taaatgaaac ggcagagcag ggaagaaggg atttagcgaa gccgcacttc cttcacaaaa
     3001 aataatgtgt gaatatttca catcgttata taaaatattt tcatcctgaa tttgtaaagc
     3061 gctgtaaata aatttttctg tttgtatttt attttcggat gcaagaacgt ttttataaga
     3121 gtcgagcaat aatttaattt tgagaaaatg ccccattgca atacacatgg caccttgtgg
     3181 agctgaaata ttttgtagcg atgattgttg gatttcgctg cattgtagtt ttgcacaatc
     3241 atcatcggca ctgcgtgcaa gccagtcgtt gcggtgcttt acatcggcat atatttcgag
     3301 tatatttttt tgttcgaaaa aagtattgcc taattgtttt gacatttcct ggtaaaacaa
     3361 ttttgcctca tgcaatgcaa cattgtattg cggtgcaatg gcaagtcgcc tgccggtaat
     3421 tgggtgcaca atgccggcag caatttgcga tgatgagctt gggttcgaat tatcgataac
     3481 gtcatgttca atttgttgtt gacttaacat aaaagataca actgtaccgg caatgccttg
     3541 tcctattatc aatatttttg gtgccggcaa cattgctaat tacgtttttg ctcaatagtg
     3601 gtttttgacg acaccggttt ggttggaatc attttttcga caattttatt ttggctatat
     3661 cctgctactc ttaaactatc attggccatt ccaataaaaa ttacagagtc taatttacct
     3721 tcgaaatcga tatcgcgcac aatggttttg ttgaaataaa aaatacgctg atacatttta
     3781 tacctgggtt gcaacgattc gttggttagc attgatgttt gtagttcgat aaaataatta
     3841 tcgcgaatat taaacttgtt ttcagcattg ctataaaaat cgaagttggc aataaaaccc
     3901 acaagtgctg atgtgttggt agcaaatagc aaaaaactta aagcataaat tgagaaatac
     3961 attcgtatga gaaaatgcaa ttttgatttt aatgcgatgc cacacaatgc cacacccgaa
     4021 caaatgaaaa gaatatttat ggtctgactt atcgatgcgt ttttttcgtt tttgaaaaaa
     4081 agaaaaacca ctatggcaac aatgccaagt acacaatgtg ctacaattat tttttttaat
     4141 ttatagatgt ttatgttttt attcagtagc aaca
//
LOCUS       DAORQA010000154         4172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5234_length_4172_cov_4.766578, whole genome shotgun sequence.
ACCESSION   DAORQA010000154 DAORQA010000000
VERSION     DAORQA010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5234_length_4172_cov_4.766578"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..>607
                     /locus_tag="PK736_07275"
     CDS             265..>607
                     /locus_tag="PK736_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HRC33230.1"
                     /translation="MNKALQPIIYAVLIVGGIFVGKYLANPEITQNIVSPGKVSGFRK
                     VGDVLKYIEDEYVDTIQETELVNSSIVSMLENLDPHSAFIPAEDFADANEGLQGNFEG
                     VGIEFHIQDDSV"
     assembly_gap    608..617
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <618..1850
                     /locus_tag="PK736_07280"
     CDS             <618..1850
                     /locus_tag="PK736_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRC33231.1"
                     /translation="PGGPSDEVGIQAGDRIVKVDGENFTGPKITNDSVMHTLRGEGGT
                     MVKVEIFRRNSNGLITINIKRGQIPLNSLDASYLLTPQTGYIKLSRFGATTFDEFHKA
                     LLDLKQKGMQNLVLDLRGNPGGYMDAAVDIADEFLEKSKMIVYTKGRSRPKQEYKATS
                     KGDFEKGKVYLLIDEGSASASEIVAGALQDWDKATIIGRRSFGKGLVQEQANLPDGSA
                     IRLTVARYYTPTGRSIQKPYEHGYSEYNNEVYERYKHGELLEKDSIKFPDSLKFKTPG
                     GKTVYGGGGIMPDYFVPLDTSFRNNLYEQLLGAGLINNAVYDYVDGNRKYLNSFIGIK
                     EYMEGFVLPDNYLKSILAKAEKEKIQYKPNELAGCKNQIAMLSKALIARQIWRTDGYY
                     RVLNQQDKTITKALELVN"
     gene            1893..2744
                     /gene="panC"
                     /locus_tag="PK736_07285"
     CDS             1893..2744
                     /gene="panC"
                     /locus_tag="PK736_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRC33232.1"
                     /translation="MKEIQIITSAHEMKNFAAECKAQHKTIGFVPTMGALHIGHISLI
                     NSSVKNCDVTVVSIYVNPTQFNNPDDLKKYPRTFDTDKAMLETTACDVIFFPSINEMY
                     PDGLITTQLDLQGLDKVMEGEHRPGHFAGVVTIVKSLFEIVNPDVAFFGEKDFQQLAI
                     IKYMTHAFNMPVEIMPCHTVREEDGLAFSSRNIHLTPDERKDAPLIYDTISQCRNKKL
                     TMTVYEVKAWATNRINSSPYLKVEYIEFVNSKTLQPLNNWDECEEQRVCTAVTVSKTR
                     LIDNTVL"
     gene            2814..3818
                     /locus_tag="PK736_07290"
     CDS             2814..3818
                     /locus_tag="PK736_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRC33233.1"
                     /translation="MNKTVVNIIKYTLMLGLGGLMLWLTFRGMDLSHIWEKIKNARFG
                     WVILSAVFSFIAFISRAYRWNMLLEPAGYKPLLKNTSYAVMFAYLANLAFPRLGEVTR
                     CGALSKKEKIPFDILFGTVIVERVLDVLSLFIAIGIVSLLEFNTMGKFIYDNFLVGMA
                     AKFTIVNMIIVLSAAVASTFVIVRIVKDKSESGFIFRFKKLLGGIGTGLKSILKIKNL
                     PLFIFHSIFIWVMYYLMIYACFFSMDATSALGVRQCFLILVVGGLAMSAPVQGGVGLY
                     HLFVSSGLALYGVPLDDGVAFATLLHTSQIIEIVLCGVVALILLGVSKDRDSKMSHAK
                     "
     gene            3808..>4172
                     /locus_tag="PK736_07295"
     CDS             3808..>4172
                     /locus_tag="PK736_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HRC33234.1"
                     /translation="MQNSVDIEHIIFDKVEDFLPLLNEWRASNFKIVFTNGCFDILHV
                     GHVDYLNKAKALGHKLVVGLNTDASVKRLKGPQRPLVAQHSRAIVIASLKFVDAVVLF
                     DEETPLQLITTIVPNVLTKG"
BASE COUNT         1324 a          716 c          887 g         1235 t
ORIGIN      
        1 atttggatga gggcatcgat tttttgcaac gctttggtgt ggaagtgcat cagtatgaaa
       61 aggaaaatag tgataccatt taaaacatga tgactaataa actgttttgc aattctgatg
      121 aacgatagaa tattttttta ttacaacact ttgcataaaa ccgaattgag attgttcaaa
      181 atctggtaaa ggcgaaatct taaacagtag aaaaacaatt tccgttcgac aaattatttt
      241 taaataagaa agaaaaaaat aacaatgaat aaagcgcttc aaccgataat atatgcggtg
      301 ttaattgtag ggggaatttt tgtagggaaa taccttgcca atccggagat aacacaaaac
      361 attgtaagcc cgggaaaagt atcgggcttt cgtaaagtgg gcgatgtgct taaatacatc
      421 gaagatgaat atgttgatac catacaggaa accgaactgg taaacagctc tattgtatcg
      481 atgctcgaaa atctcgatcc gcattctgca tttatacctg cagaagattt tgctgatgcc
      541 aacgaaggtc tgcaaggaaa ttttgaaggc gtgggtattg agtttcatat acaggacgac
      601 agtgttannn nnnnnnnccg ggcggaccat cggacgaagt gggcattcag gccggtgaca
      661 gaattgtgaa agtagatggt gaaaatttta caggaccaaa aattaccaac gatagtgtga
      721 tgcatacgct gcgtggtgaa ggtggcacca tggttaaagt agaaattttc aggcgtaatt
      781 caaatgggct tataaccata aatataaaac gtggacaaat tcctctcaat agtctcgatg
      841 catcttatct tcttacacca caaacaggat atataaaatt gagcagattt ggcgcaacca
      901 catttgacga atttcataaa gcgttgctcg atctgaaaca aaaaggcatg caaaatcttg
      961 tgcttgattt gcgtggtaat cccggtggat acatggatgc cgctgttgac attgccgatg
     1021 agtttttaga aaaatcgaaa atgattgttt acaccaaagg acgttcgcga cccaagcaag
     1081 aatataaagc tacttcgaaa ggcgatttcg aaaagggcaa agtttattta ttgatagatg
     1141 aaggcagtgc atcggcatcg gaaattgtgg ccggtgcgtt gcaagactgg gataaggcta
     1201 ccatcattgg ccgcaggtcg tttggcaaag gccttgttca ggaacaggca aacctgcccg
     1261 atggttcggc catacgcctt accgttgcgc gatattatac tcccacgggg cgcagcattc
     1321 aaaaacccta cgagcatggt tacagtgaat ataacaacga ggtttacgag cgatataagc
     1381 atggtgaatt attggaaaag gatagtatta aatttcctga ttcattgaag tttaaaacgc
     1441 caggtggtaa aacagtgtat ggtggtggtg gcattatgcc cgattatttt gttccgctcg
     1501 atacttcgtt tcgcaataat ttatacgagc aacttttggg tgccggcctt atcaataatg
     1561 ctgtatatga ttatgtagat ggcaatcgaa aatatctcaa ttcgtttata ggaataaaag
     1621 aatatatgga aggctttgtg ttgcccgata attatttgaa atcaatttta gccaaagccg
     1681 aaaaagaaaa aatacaatac aaaccaaacg aactggccgg atgtaaaaac cagatcgcta
     1741 tgttaagtaa agcgcttata gcacgtcaaa tatggcgtac agatggatat taccgcgtat
     1801 tgaatcagca agacaaaacg attaccaaag cactggaact ggtaaattaa attattgcaa
     1861 tttttcattt aagaaaatcc taaaagcatc agatgaaaga aattcagata atcacttcgg
     1921 cccatgaaat gaaaaatttt gccgctgagt gcaaggccca acacaaaaca attggttttg
     1981 ttcccactat gggtgcatta catattggcc atataagcct cataaatagt tcggtaaaaa
     2041 attgtgatgt tacggtggta agtatttatg taaaccctac acaattcaac aaccctgacg
     2101 atttaaaaaa atatccccgc acattcgata ccgataaagc catgcttgaa accacagcct
     2161 gcgatgttat tttttttcca tcgataaatg aaatgtatcc tgatggactg ataacaactc
     2221 agcttgactt acaagggttg gataaagtga tggaaggaga acacagaccg gggcattttg
     2281 ccggtgtggt tactattgta aaaagccttt tcgaaattgt gaatcccgat gtagcttttt
     2341 ttggcgaaaa ggattttcag caattggcca tcataaaata tatgacacat gcatttaata
     2401 tgcctgtaga gattatgcct tgccatactg ttcgcgaaga agatggcctt gcttttagct
     2461 cgcgcaatat tcaccttaca cccgatgaac gtaaagatgc accactgatt tacgacacca
     2521 tttcgcaatg caggaacaag aaattaacta tgactgtata cgaggtaaaa gcctgggcca
     2581 ccaaccgcat aaattcttca ccttacttaa aagtggaata catagaattt gtaaattcga
     2641 aaacattaca gcctttaaat aactgggacg aatgcgaaga gcaacgtgta tgcactgcag
     2701 ttactgtttc gaaaacacgc ctcattgaca atacagtgtt gtaaaacgaa ttttaaaatt
     2761 tacttttgtt acataacgat ttttataatt gcttaaacac aaaaaatata tagttgaata
     2821 aaactgttgt aaacattatc aaatatacac ttatgcttgg cctgggcggg cttatgctgt
     2881 ggcttacttt ccgaggtatg gacttaagcc atatttggga aaaaataaag aatgcccgct
     2941 ttggatgggt tattctttct gcggtttttt cattcatcgc ttttattagc cgtgcctacc
     3001 gctggaatat gttgctggag cctgccggat acaagccact cttaaaaaac acttcgtatg
     3061 cagttatgtt tgcctatctg gccaacctcg cctttccccg gctgggtgaa gtaacgcgat
     3121 gtggtgcatt aagtaaaaaa gaaaaaatcc ctttcgatat tttgttcggt actgtgatag
     3181 tagaaagggt gcttgatgtt ttatcgctct ttattgctat tggtattgta tcgttgctag
     3241 agtttaatac gatgggcaaa tttatttatg ataatttttt agtaggtatg gcagcaaaat
     3301 ttacaattgt caatatgata atagtattga gtgcggctgt ggcttctact tttgtaatag
     3361 taagaatagt aaaagacaaa tcggaaagcg gatttatttt tcggttcaaa aaattgcttg
     3421 gtggcatagg cacaggattg aaaagcatat tgaaaataaa aaaccttcca ttatttattt
     3481 tccattcaat atttatttgg gtgatgtatt accttatgat atacgcatgt ttcttttcga
     3541 tggatgccac atcagcattg ggtgtgcggc aatgcttttt aattcttgtt gtaggtggcc
     3601 ttgccatgtc ggcaccggtg cagggcggtg tggggctgta tcatctgttt gtatcaagtg
     3661 gcttagcatt atatggtgtt ccgctcgatg atggcgttgc ctttgccaca ttactgcata
     3721 cttcacaaat aatagaaatt gttttgtgtg gtgtggtagc gttaatatta ttgggagttt
     3781 caaaagacag agatagtaaa atgagccatg caaaatagtg tagatattga acatattatt
     3841 ttcgacaaag ttgaagactt tttgccattg ctaaatgaat ggcgcgcatc aaatttcaaa
     3901 atagttttta caaatggatg cttcgatatt ttgcatgtag gtcatgttga ttatttaaac
     3961 aaagcaaaag cactggggca taaattagta gtaggattga ataccgatgc ttcggtaaaa
     4021 agattgaaag gtccacaacg ccctttggtg gcgcaacatt cgcgtgcaat agtaattgct
     4081 tctttaaaat ttgttgatgc cgttgttttg ttcgatgagg aaaccccctt gcaattaatt
     4141 acaacaatag ttccaaatgt acttactaaa gg
//
LOCUS       DAORQA010000155         4162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5254_length_4162_cov_23.616995, whole genome shotgun sequence.
ACCESSION   DAORQA010000155 DAORQA010000000
VERSION     DAORQA010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5254_length_4162_cov_23.616995"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PK736_07300"
     CDS             complement(<1..412)
                     /locus_tag="PK736_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRC33235.1"
                     /translation="MKIYIKNMVCNRCIMVVKNELEKLGFQTLNISLGEVELKNNLTE
                     TEKTNLDNHLKTFGFELIDDKKSRLIGQIKSYIIEIIHQNNSELKSNLSDYLSSKLHH
                     DYTYLSNLFSEVEGTTIEKYFIAQKIEKVKELLVY"
     gene            complement(810..2252)
                     /locus_tag="PK736_07305"
     CDS             complement(810..2252)
                     /locus_tag="PK736_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRC33236.1"
                     /translation="MKWKAQIIEHRNQSRIAVYFDKNNDAIARIKKLPDAKWSQTWKA
                     WHLPNNEENRLRFKLADTHAAPIHAQKHNAINTTIAIPQPHVSNNEPNLDAATPMVQT
                     EVHIHATDKKIIIKLPKNETDTAFITSLRFSTWHKKLLHWIIPNYGNNLQRIKIHFGN
                     RIATMQIITNTNESTIENAAATKTDSKHLQLQFTKVLLSEVCKLKVDKYILWLKSKRY
                     SQNTIKVYTEALTTFLKYYSNKAVHEINNEDVIMFNNEYILKYNLSASYQNQVVNAIK
                     LFFSKIQHTAMDINLVHRPKRAFLLPNVLSKEEVQKIFNATDNIKHKTMLCLIYSCGL
                     RRSELLNLMPQNIDRSRGLLIIKQSKGRKDRITPLPNKIMQMLETYFKQYLPKKWLFE
                     GQTAGSQYSERSLNLVFAGAVEKSLIKKPATLHWLRHSYATHLLEGGTDLRYIQELLG
                     HSSSKTTEIYTHVSSKKISEIKSPIEDLDI"
     gene            2649..3041
                     /locus_tag="PK736_07310"
     CDS             2649..3041
                     /locus_tag="PK736_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRC33237.1"
                     /translation="MPHQQIELILSRQLADNLSIPIFIVDTVGNLLFYNEPAEELLGL
                     RFEETGAMPVEEWSTIFKPQDDKGEILPASALPLVQTIKKQEPAHGSFWIRSMKGESF
                     KIAVTSYPIIGRPHRFLGAVALFWKSVI"
     gene            3038..3880
                     /locus_tag="PK736_07315"
     CDS             3038..3880
                     /locus_tag="PK736_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC33238.1"
                     /translation="MMNIKIWGCRGSLPAPGTDKDIYGGNTSCIEIIDGDTCIILDGG
                     SGLQRLNNYLPPNITRIDILLTHLHLDHIMGLGFFWPLYNPNMTVNIWGPAASNESLK
                     QRLRRYFSPPIFPVRLNELPCKGELFEIDSSEFQIGNFKITSEYVCHPGPTVAYRLQT
                     GNTVIAYIPDHEPALGSAQFPTNAEWTSGFSIAEGADILFHDAQYSSEEYKTRVGWGH
                     SSVEDALDFGTLAKVKKIVLFHHDPAHTDDNLKTMLAETLEEKKCDFEVEIATEGTSF
                     EFES"
BASE COUNT         1118 a          755 c          864 g         1425 t
ORIGIN      
        1 cataaaccaa aagttctttc accttctcaa ttttttgagc aatgaaatat ttttctatag
       61 tcgttccttc aacttcggaa aacaggtttg ataagtaagt atagtcgtga tgaagcttac
      121 tgctcaaata gtcagataaa ttggatttta attcgctgtt attttgatgt ataatttcaa
      181 taatgtatga tttaatttgt ccgattagac gacttttttt gtcatcaatc aattcaaatc
      241 caaacgtttt gagatgattg tcaaggttag ttttttcagt ttcagtcaag ttgtttttga
      301 gttcaacttc gcctaaacta atattcaaag tttgaaaacc tagtttctcc aattcatttt
      361 tcactaccat tatgcagcga ttacaaacca tatttttgat ataaattttc atatgttaat
      421 gtttatgtcg tgcgttggca aaaagtggct cttttggttt tgctgaaggg tcggctttgt
      481 gtgtcggggc aaaaccaaat gtgccacttg tgcggatggc taaaattgtt tttaaaaaag
      541 tgcggtgggg aaaaaaataa aaaacatagg gtaggcaatg agtgtcggct tactcgttgt
      601 ctgtttgtaa tatcgtttct atgtctttgg tcacctgtca aaatggttta catttttctt
      661 gtttttagtc gtctgtttga tgtttgaacg gtcgtcctac cattggctat aactagtaga
      721 ttggagcagt ttttaatcct aaaccttctc caatctgtcc aatgtgggca gatataggaa
      781 ggttttggtt gcgaacatag ttataatatt tatatatcca agtcttcgat agggcttttt
      841 atttcgctta tctttttact gcttacatgg gtataaatct cagttgtttt gctactgcta
      901 tgcccaagaa gttcttgtat ataccttagg tcggtgccac cttcgagtaa atgggttgcg
      961 tagctgtgtc tgagccaatg tagggttgcg ggctttttga tgaggctttt ttcaacggca
     1021 ccagcaaaaa ccaaatttaa actccgctcg ctatattggc tgcctgcggt ttgcccttcg
     1081 aacaaccatt ttttgggtaa gtattgttta aagtatgttt ctagcatttg cattattttg
     1141 tttggcaaag gggttattct gtcttttcgt cctttggatt gttttataat caataggcca
     1201 cggctgcggt caatattttg tggcattagg tttaagagtt cacttcgcct gagcccacag
     1261 ctatatatta ggcataacat tgttttgtgt ttgatattat cggtggcatt aaatatcttt
     1321 tgcacttctt ccttgctaag tacatttggc aacagaaaag cacgcttggg cctgtgcacc
     1381 aaattgatat ccatagcggt atgctgtatt ttgctaaaga ataattttat ggcattcact
     1441 acttgatttt gatacgaagc cgaaagatta tatttcaaaa tgtattcgtt attaaacata
     1501 attacatcct cattatttat ttcatgcact gctttattgc tataatattt taagaaggtg
     1561 gttaatgcct cggtatatac ttttattgta ttttggctgt atcgtttcga tttcaaccaa
     1621 agtatgtact tatccacttt tagtttgcac acttcactca acaatacttt tgtaaactgc
     1681 agttgcaaat gtttagagtc tgtttttgta gcagccgcgt tttcaattgt gctttcattg
     1741 gtgttagtta ttatttgcat tgtagcaatg cgattgccaa aatgtatttt gatacgttgt
     1801 agattgttgc cataattggg tattatccaa tgtaataatt ttttgtgcca tgtactgaat
     1861 ctaagtgagg taataaatgc agtatcggtt tcgttttttg gcagcttaat tattattttt
     1921 ttatctgtcg catgtatgtg cacctctgtt tgtaccattg gggtggcggc atcaaggtta
     1981 ggttcgttat tgcttacatg cggttgcggt atggcaatgg tggtatttat tgcgttatgt
     2041 ttttgtgcat gtatgggtgc tgcatgggtg tcggctaatt taaatcttag tctgttttct
     2101 tcgttattgg gcaggtgcca tgccttccat gtttggctcc attttgcatc gggcagtttt
     2161 tttatgcgtg caatggcatc attattttta tcgaagtaaa cagcaatgcg cgattggttt
     2221 cggtgttcaa ttatttgtgc tttccatttc atgatgagca taaaattttc tataattttt
     2281 caaaaataaa aaattaaatc ggatggtgat ttaattattt agcatcgcca ccccacaaag
     2341 ccgcaacccc ctgcccatag ccccgattgc agctgttatc gtatgtcatg ccaataaaaa
     2401 acaaaaaaaa acaatggcat acataaaagc ggaaagcggg accggccgtt tgtaagagga
     2461 tgaactattc tgcttcacat catttatttt gttttatatt ttatagtgtt gttgatttag
     2521 atttcacact atggactgct gctgtaataa cccacaattt ttttcaaact cgcttgcggg
     2581 tataataaaa taattagatt tgtgcagaca tttacctcgg cacaaaactt ctaaccgaaa
     2641 atatttatat gcctcaccaa caaatagaat taatattaag ccggcagttg gccgataatt
     2701 tatcgatacc tatttttata gtggataccg ttggcaatct gcttttttac aacgagcctg
     2761 ccgaagaatt gcttggtctg cgctttgaag aaaccggtgc catgccggta gaagagtggt
     2821 ctaccatttt taaaccgcag gatgataagg gcgaaatttt acctgcatcg gcattgcctt
     2881 tagtacaaac aataaaaaag caagagcctg cccacggttc tttttggata cggagtatga
     2941 aaggggagtc atttaaaatt gcggtaacgt cataccccat cataggccgt ccacataggt
     3001 ttttgggtgc tgtagcatta ttttggaaat cggtaatatg atgaacatta aaatttgggg
     3061 ttgccgtggt tcgcttcctg caccgggtac cgataaggat atttatggcg gaaatacttc
     3121 gtgtatagaa attattgatg gtgatacttg cattattctc gatggtggtt cgggcctgca
     3181 acggcttaat aattacctac caccaaatat tacccgcatc gatattttac ttacacattt
     3241 gcacctcgac catattatgg gtttgggatt tttttggcca ctgtataatc caaacatgac
     3301 cgtaaatatt tggggccctg ctgcttcaaa cgaaagtctt aagcaacggt tgcgcagata
     3361 tttttcgcct ccgatttttc cggtacgatt aaacgagttg ccctgtaagg gagaattgtt
     3421 tgaaattgat agcagcgaat ttcaaattgg aaattttaaa atcacctcgg aatatgtttg
     3481 tcatcccggc cctacggtgg cttacaggtt gcaaaccggc aatactgtta ttgcttatat
     3541 tccggatcat gaaccggctt tgggttcggc tcaatttcct actaatgcag agtggacaag
     3601 tggatttagt attgccgagg gagcagatat tttgtttcac gatgcgcagt atagcagcga
     3661 agagtataaa acacgagtag gatgggggca cagttcggta gaggatgcac ttgattttgg
     3721 cacgcttgcc aaagtaaaaa aaatagtgct gtttcaccac gaccccgcgc acaccgatga
     3781 taatttgaag acaatgcttg ccgaaacttt agaagaaaaa aagtgtgatt ttgaagttga
     3841 aattgctacc gaaggaacat cgtttgagtt tgagtcttag ttttttttgc cacgcctgcc
     3901 ccgaccgcag tgtcggggaa tgcacgaatg aatgccgaat aatactttgc cacacctgcc
     3961 ccgaccgcag tgtcggggaa aaacactgaa tgcactaaag aatgctgaat aatattttgc
     4021 cacgaaaaac actgaatgca ctaaagaatg ctgaataata ttttgccacg cctgccccga
     4081 ccgcagtgtc ggggaaaaac actgaaagca cgaatgaatg ccgaataata ctttgccaca
     4141 cctgccccga ccgcagtgtc gg
//
LOCUS       DAORQA010000156         4121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5315_length_4121_cov_65.737580, whole genome shotgun sequence.
ACCESSION   DAORQA010000156 DAORQA010000000
VERSION     DAORQA010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5315_length_4121_cov_65.737580"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PK736_07320"
     CDS             <1..486
                     /locus_tag="PK736_07320"
                     /inference="COORDINATES: protein motif:HMM:NF014102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33239.1"
                     /translation="NVGGEEAVDVVRICLENNGKCLDQDIEERMKTKKITEIRSILNR
                     LHYRGIACYNKTKNKNSGWYTYTWEIKERRIVELILEQQAEQLQKLEQKAKFDGAHEI
                     FVCKKGCEDTAFEIAAQYEFRCPECGEMMNPVDGKKRQKEIQTQIKEITEELKQLEKT
                     V"
     gene            598..672
                     /locus_tag="PK736_07325"
     tRNA            598..672
                     /locus_tag="PK736_07325"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:633..635,aa:Met,seq:cat)
     gene            1031..1501
                     /locus_tag="PK736_07330"
     CDS             1031..1501
                     /locus_tag="PK736_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33240.1"
                     /translation="MRRVFKSLRTIQEWAEDGTVSESTNQERAINYVSKGNKNVNTLT
                     QMSVPQYKVINVWNYLKFKEGSQAHHITKTLDFEEWITMEEILRRVNELFGVQYQNER
                     SLYPYLKTLVDAGLLEASMLGGKMRWRKKNLMQTINLMVEEIENEAVVPSQPLQ"
     gene            complement(1484..2092)
                     /locus_tag="PK736_07335"
     CDS             complement(1484..2092)
                     /locus_tag="PK736_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33241.1"
                     /translation="MGGGIVANAYYIQYKFILPDSIKHSTYTYQKLFRAIYGYTQNVT
                     KSNGKVYHYHRKGVLSGTPYVRPGKNAVIIHPSAFPKIKQFFTTGQNPTHNWRSKGDW
                     KAVYYMDEKEVDDAKLVSALEDWVDRTYITANERYPTKITEEVSTILDKQKVGETPEE
                     SHKSRVLNEANSIISSDWFKSHHQKSQKLIDFHEKVKSLKGL"
     gene            complement(2212..2535)
                     /locus_tag="PK736_07340"
     CDS             complement(2212..2535)
                     /locus_tag="PK736_07340"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HRC33242.1"
                     /translation="MTVRLSKLFGMDIYTDNAEYKGKIFDLVINLEKGKIETMTTEPL
                     KVRTKQEARKIISEKSIPYQNVLSAKDILLVGTRSRPTPVEEPEEQPTTALRRPSYQP
                     YTRRR"
     gene            complement(2926..3963)
                     /locus_tag="PK736_07345"
     CDS             complement(2926..3963)
                     /locus_tag="PK736_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRC33243.1"
                     /translation="MYFILQNDYAGMDDDNRLPVSEQHPAMLEKSEAPLALFEKNNTL
                     ETNGAESKQTSSDETILPARGHAFSVQHEKILTQFKQELLVTGYSPRTIKMYLVYVQK
                     LLEETPKMPQEMDKQDIVGFLARAREKNVSNATLSLMHSALKFFFQQHIGKKIIDDIK
                     IPKKGKKLPSVLTVNELKDIFKNTKVGRNRLLLQFIYSTGVRVSEAVNMRVNELDFDN
                     STALVKSGKGNKDRVVVLSQKWITFIKKYLNRKKVQSEYLFSKKNGKKISTDTVERII
                     RHSAKESGISKRVTPHVLRHSFATHLLESGENIRKIQILLGHANLSTTSIYTHVSKEE
                     LRKVQSPLDNM"
BASE COUNT         1208 a          759 c          809 g         1345 t
ORIGIN      
        1 aatgtggggg gagaagaggc cgtggatgtc gtgcgaatct gcttggaaaa caacggaaaa
       61 tgtttggacc aagacatcga agaacgaatg aaaacaaaaa aaattacgga aatacgaagc
      121 attctcaacc gattacacta tcgtggaatc gcgtgttata acaaaacaaa aaacaaaaac
      181 tccggatggt acacgtacac atgggagata aaagaaagga gaattgtgga gctaatttta
      241 gaacagcaag ccgagcaatt gcagaagctt gaacaaaagg caaagtttga tggcgcacat
      301 gaaatttttg tatgcaaaaa aggatgcgaa gatacggcat ttgagattgc cgcgcagtat
      361 gaatttcgct gccctgaatg cggggaaatg atgaatccag tggatggaaa gaagagacaa
      421 aaagagattc aaactcaaat caaagaaatt acagaggagt tgaaacaatt agaaaagaca
      481 gtttaacgca caaacttgga caaggcactt ttattataag gtgattgttc caaaagttaa
      541 tctgcctaaa aataaggaat agaaggcaaa tagaaagtaa ttaattaacg tgaaaatagc
      601 ggggcggggc agcctggagt gcccgtcggg ctcataaccc gaagatcgaa ggttcaaatc
      661 cttcccccgc tactcattat tttactcttt ttctaaccgg aatgaaagcc tgaaaaaagg
      721 cctctttttt gtgactcatt attcaacttt tcgagttttt ggcctatttt ttgaaaactt
      781 tgtatctcat ttagcatttt tttcatatcg gcacctcgtt ttttaacaat cctttgtttt
      841 tagttgaatg ttatattatt ttgctgcact cattcgacaa attttggtta tttttggtaa
      901 ttttattaac attcttcgta attactgtaa agattaaact tacttatttg tttttggtaa
      961 ttactagtaa ttaccataag gtttttaagt aattactatc attaccatca ttaccatgat
     1021 taaactgatc ttgagaaggg tgttcaagag ccttagaacc atccaagagt gggcggaaga
     1081 cggcactgtg agtgagagca ctaatcaaga acgagcaatt aactatgttt caaaaggcaa
     1141 caagaatgtt aacacattaa ctcaaatgag tgtaccccaa tataaggtca ttaatgtgtg
     1201 gaactatttg aagttcaaag aggggagtca ggcccaccat attacgaaga cattggattt
     1261 tgaggaatgg attacaatgg aagagatact gcgtcgcgtg aatgagttat ttggagtgca
     1321 gtatcaaaat gaacgcagcc tttacccgta tttgaagaca ttggtggatg cggggttgct
     1381 tgaggccagt atgttaggcg gcaagatgag atggagaaag aagaacttga tgcagacgat
     1441 taatttgatg gtagaagaaa tagaaaatga agcggttgtg ccttcacaac cccttcaatg
     1501 acttcacttt ttcatggaaa tcaattaatt tttgagattt ttggtgatgc gacttgaacc
     1561 aatcgcttga aatgattgaa ttggcttcat tcaaaacacg cgatttatgg ctttcctcag
     1621 gggtttcgcc aaccttttgc ttgtccaaga ttgtgctcac ttcttctgtt attttagtgg
     1681 gataacgctc atttgccgtt atataggtgc gatccaccca atcttctaag gcagaaacaa
     1741 gcttagcatc atctacttct ttctcatcca tatagtagac tgctttccaa tcccccttcg
     1801 atctccaatt atgagtgggg ttttgacctg tagtaaaaaa ttgttttatc ttaggaaaag
     1861 cagaaggatg aataataact gcattcttac caggtcgaac ataaggggtt cctgataaaa
     1921 ctccttttcg gtggtaatga tagactttac cattcgattt agtcacattc tgcgtatacc
     1981 catagatagc tcgaaatagc ttctgatacg tgtacgtaga atgtttgata ctgtctggca
     2041 agatgaactt gtattgaatg taatatgcat tagccactat accacccccc atacttccgg
     2101 tattggtttg taaaaattat atgtaaaata tatttgctta tgttgcgtta taagacataa
     2161 agactattta tagatttgga ttagacggtc gcctaaaaaa ataattgcct gctatcggcg
     2221 tcgagtatac ggctgatagc tagggcggcg taaggcagta gtgggttgct cttcaggttc
     2281 ttcaaccggc gtcggtcgag agcgagttcc aaccaacaaa atgtctttgg ctgaaagaac
     2341 gttttgatag gggatgctct tctctgaaat aattttgcga gcttcctgtt ttgttcgaac
     2401 ctttaagggc tcagtggtca tggtttcaat ttttcctttt tcaaggttga taaccaaatc
     2461 gaaaatttta cctttgtatt ctgcgttgtc tgtatagatg tccattccaa atagctttga
     2521 taggcgtacc gtcataatta ttcccccttg aatttctcaa gaaatcaagt gcaacataga
     2581 acccgaattt cgtatataaa tcttgtgcaa atgtttgagt aaaaacggta tttgccatgc
     2641 ataaatagaa gcggaagtga aggcggggtg tgttttatgc atattttttg atcaaaaccc
     2701 caaaaatttt tgcgcgactg attggttgtg ttcattatta gtggaagtaa gggggggcac
     2761 gttgttaatt tttgggaagt ggaaaaacaa aatataaatt atgtataata ttaatacata
     2821 actttctatt tttttgttac acacccaaaa ttagtttttt ggtgctatat ttttttagga
     2881 tttttttatt ttagttattg ataactaact tcttttcaaa taattctaca tgttatccag
     2941 tggactctgc acttttctca actcttcctt gctcacgtgc gtgtaaatag atgtcgtgct
     3001 caaattagca tgccctaaca aaatttgaat ttttcgaata ttttctccgc tttcgagtaa
     3061 atgcgtagcg aagctatgtc tcaacacgtg cggggtgact cttttactta ttcccgattc
     3121 ttttgctgaa tgacgaatga tgcgctccac tgtatcagtt gaaatttttt tcccattttt
     3181 tttgctaaat aaatattcac tctgcacttt ttttcgattc aaatattttt tgataaatgt
     3241 aatccatttt tgcgacaaca ccactacgcg atctttattt ccttttccac tcttgacaag
     3301 ggcggtcgaa ttatcaaaat ccaattcatt tacccgcata ttcaccgctt cactcacgcg
     3361 tacgccagtg gagtaaatga attgtaatag caaacgattt cttcctactt tcgtattttt
     3421 gaaaatgtct ttcagttcat ttaccgttag cacagaaggc aatttctttc ctttttttgg
     3481 aattttaata tcatcaataa ttttttttcc aatatgctgc tgaaagaaaa atttcaatgc
     3541 tgagtgcatt aacgaaagag tggcatttga aacatttttt tcgcgcgctc tcgccaaaaa
     3601 gccaacaata tcctgcttat ccatctcttg cggcatttta ggagtttctt ccaacaattt
     3661 ttgtacgtac actaaataca ttttgattgt gcgtggagaa taacccgtca ccaaaagttc
     3721 ctgcttgaat tgggtcaaaa ttttttcgtg ctgaacacta aacgcatgtc cgcgcgcggg
     3781 caaaatagtc tcatcgctgc tcgtttgttt tgattctgcc ccatttgttt caagggtatt
     3841 atttttttca aaaagggcga gaggtgcttc actcttttca agcatcgcgg ggtgttgttc
     3901 actcaccggc aatcgattat catcatccat tcctgcataa tcattttgca ggataaaata
     3961 catgcgcttg ggttgcttta ttcgtcttta ggcttttctt ctgtagcggc agaaccggct
     4021 tcttcttcca attcctgcaa aaccttgctt ttcttgccct ttttgacttc ttcaaagtct
     4081 tccactaaga cttcggcctc ttcatcgtct aaacccagca a
//
LOCUS       DAORQA010000157         4104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5333_length_4104_cov_8.455421, whole genome shotgun sequence.
ACCESSION   DAORQA010000157 DAORQA010000000
VERSION     DAORQA010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5333_length_4104_cov_8.455421"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PK736_07350"
     CDS             <1..317
                     /locus_tag="PK736_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33244.1"
                     /translation="TIVYKTKKRLALEAGLVYARIVATYEGDDTGEFKDQLPFKKNEV
                     SIQASLDFKLSPNFTIQVLAQNSLTPIRTNGVKSGIYLGQYNSILGFNVHYFFAHKQT
                     QQ"
     gene            314..877
                     /locus_tag="PK736_07355"
     CDS             314..877
                     /locus_tag="PK736_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HRC33245.1"
                     /translation="MKHKVIVAVTGASGAIYAKVLFDKLILLQPQIEQVGVVFSGNAK
                     EVWQYELGNKDYENIPFKTFGSQDFNAPFASGSARYNVMIVCPCSMGTLARIATGISN
                     DLITRAADVVLKERRKLILVQRDTPLSLIHINNMKSITEAGGIICPASPSFYSKPATF
                     EMLAATVVDRALELAGLQVDTYRWNEK"
     gene            942..1427
                     /locus_tag="PK736_07360"
     CDS             942..1427
                     /locus_tag="PK736_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRC33246.1"
                     /translation="MAILINTGITLGTFLLMECITWCTHKYVMHGFLWYLHKDHHQPH
                     DGFFEKNDAFFLIFAVPSFLLMLTGAQAGFDFKFALGLGIMLYGMAYFLVHDIFIHQR
                     FKLLRNANSWYARGLRKAHKVHHKHLGKYEGECFGMLFVPLQYFREAMKRSDNLFEKG
                     R"
     gene            1542..1613
                     /locus_tag="PK736_07365"
     tRNA            1542..1613
                     /locus_tag="PK736_07365"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1575..1577,aa:Arg,seq:ccg)
     gene            complement(1806..>2083)
                     /locus_tag="PK736_07370"
     CDS             complement(1806..>2083)
                     /locus_tag="PK736_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33247.1"
                     /translation="NYLIRPINRTAMKADAKKKICDFRVFSGKKITRLKPIANYLIRP
                     INRTAMKADAKNFVPSCLCGKKIFGIHKPALESSFVLVFYLHERLES"
     assembly_gap    2084..2183
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2363..3565)
                     /locus_tag="PK736_07375"
     CDS             complement(2363..3565)
                     /locus_tag="PK736_07375"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRC33248.1"
                     /translation="MFSNLSQEALQLLAMNIRYKDVPAHEIVLNKGDAGNSMYIVING
                     QVQIHDEQHVVTTLNAGDYFGELSLLDTEVRSMSVTAVTDSSLLEITHNSFYLLIQNH
                     PNVGHHIIPELTKRMREQSKQIIVQLKNKEAELQELVNQRTMQLQLKNKEIVDSIHYA
                     KHVQEAIMPSVHLIEKHFKDSFVLYLPKDIVSGDFYWMTHTENKILIAAADCTGHGVS
                     GALMSMLGVSLLDQIVNGKKITTPSEILNQLHIAIVESLHQQNTQINNGMDICLCCFD
                     LENNVLQFSGANRPLIQIANGELIMIAADKVPIGGTQIDRPATYTNHVITLNVGNQFY
                     LTTDGYADQFGGEAGKKLMTKKLKEMIVDMRELSMTEQKKYLENYFNTWKNKYEQVDD
                     VLVIGVCV"
     gene            complement(3617..>4104)
                     /locus_tag="PK736_07380"
     CDS             complement(3617..>4104)
                     /locus_tag="PK736_07380"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRC33249.1"
                     /translation="NPAEFLVAVNEGIIYSLPQNQRDSNDGMDVSILAFDLNKRTVDF
                     AGANRPMWLIRNGELLTYKPNKFPIGGMQIIHEEKFSQHHIQLQPNDTIYIFSDGYAD
                     QFGGEHGKKLMTKRFKEILVSIQHLSMLQQQLHLKRLFEKWKGQHSQVDDVLVIGIKI
                     P"
BASE COUNT         1178 a          788 c          780 g         1258 t
ORIGIN      
        1 taaccattgt ttataaaaca aaaaaaagat tagcactcga agccggttta gtatatgccc
       61 gcattgtggc aacatatgaa ggtgatgaca ccggagaatt taaagaccaa ttgccattta
      121 aaaaaaacga agtgagtata caggcatcgt tagattttaa attatcgcca aattttacca
      181 ttcaagtatt ggcacaaaat tcgttaacac ccattcgtac aaatggcgta aaaagtggta
      241 tttaccttgg gcaatacaat tcgattttag gattcaatgt gcattacttt tttgcacaca
      301 aacaaacaca acaatgaaac ataaagtaat agtggccgtt acgggcgcga gtggtgccat
      361 atatgcaaaa gtattgttcg ataagttgat actgttgcaa ccccaaatag agcaagtggg
      421 agttgtgttt tcgggcaatg ctaaagaagt atggcagtac gaattgggaa ataaagatta
      481 cgaaaatatt ccttttaaaa cgtttggtag tcaagatttt aatgccccgt ttgcatcggg
      541 ttcggcaagg tataatgtga tgatagtgtg cccatgcagt atgggaacct tggcacgtat
      601 tgccaccggc atatcgaacg accttattac ccgcgctgcc gatgtggtgc taaaagaaag
      661 gcgcaagctg attcttgtac aacgcgatac accattgagt ttgatacaca tcaacaatat
      721 gaaaagcatc accgaggccg gaggaattat ttgccctgca tcgccctcgt tttacagcaa
      781 acccgccact ttcgaaatgc ttgctgctac cgttgtggac cgcgccctcg aacttgccgg
      841 tctgcaggta gatacctatc gatggaacga aaaataattt tgcaaccata ctcaactatt
      901 ttgttatact tttgttgaca gattaaacag aactaaacgc gatggcaata ttgataaaca
      961 caggtataac actcggcaca tttttattaa tggaatgcat aacatggtgc acacacaaat
     1021 atgtaatgca tggttttttg tggtacttac ataaagatca tcatcaacct cacgatggtt
     1081 ttttcgaaaa aaacgatgct ttctttttaa tttttgctgt gcctagtttt ttgcttatgc
     1141 ttacgggtgc acaggcaggc ttcgatttta aatttgcatt aggattagga ataatgttgt
     1201 atggcatggc ttactttctg gtgcacgata tttttattca tcagcggttt aaacttttac
     1261 gcaatgccaa tagttggtat gcacgcgggt tacgtaaggc gcacaaagtg catcacaaac
     1321 accttggcaa gtatgaaggc gaatgcttcg gaatgttgtt tgtgcctttg cagtattttc
     1381 gcgaggcgat gaaacgcagc gataatcttt tcgaaaaagg gagataaatc tttggtttcg
     1441 attttaagta gcatacccaa atttcttttt tatttagaga tgcgattgag taacttatac
     1501 tatttttgcg ggcgcaacaa tattcggtgt tgtattgatt tggtcctgta gtacaatgga
     1561 tagtacgtag gtctccgaaa cctaagatcc aggttcgatt cccggtggga ccacttttta
     1621 ttagcaaaat gctttgccga gaaatccact aagtagcaaa attgatttat aattggaatg
     1681 ctgaggaacg aagcatccct actccgaaat attttttgcc gcgaatgcgc gaataaattt
     1741 ttgccacgcc tgccccgacc gcagtgtcgg ggaatgcacg aattcaattt cgttcttacc
     1801 actaattagc tttcgagtct ctcatgcaaa taaaaaacta aaacgaatga ggactctaaa
     1861 gcgggtttat gaatgccgaa tatttttttg ccacaaaggc acgaaggcac aaagtttttt
     1921 gcgtcagctt tcattgccgt ccgatttatc ggacggatta aataatttgc aattggcttt
     1981 agcctcgtaa tttttttgcc actaaaaaca cgaaagtcac aaattttctt ttttgcgtca
     2041 gctttcattg ccgtccgatt tatcggacgg attaaataat ttgnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnncaattgg ctttagcctc gtattttctt gccacgcctg
     2221 ccccgaccgc agtgtcgggg aatgcacgaa tgaataccga ataaatgcaa aaactgtttg
     2281 gccaagaaaa agcaattttt ttttaatggt aaataaccca ataacccaat aacccaataa
     2341 ctcaataacc caacgccaac aactaaacac aaacaccaat cacaagcaca tcatctactt
     2401 gttcgtattt gtttttccag gtgttgaaat agttttctaa atattttttt tgttctgtca
     2461 tagaaagttc gcgcatgtct acaatcattt cttttaattt ttttgtcatc agttttttgc
     2521 cggcttcacc accaaactga tccgcatagc catcggtggt aagataaaat tgattcccaa
     2581 catttaatgt tataacatgg tttgtatacg tagcaggccg gtctatttgt gtgccaccaa
     2641 taggaacttt atcggcagca atcattatca attctccatt tgcaatttgt atcaatgggc
     2701 gattggctcc cgaaaactgt aatacattat tttccaagtc gaagcaacac aggcaaatat
     2761 ccatgccatt atttatttgt gtattttgct gatgcaatga ttctacaata gcaatgtgta
     2821 gttggtttaa tatttcgctt ggcgttgtta tttttttgcc atttactatt tggtcgagca
     2881 gcgatacgcc aagcatactc atcaatgcac ccgatacacc atgccctgtg caatcggcag
     2941 cagctatcaa tattttattt tcggtgtggg tcatccaata aaaatcgccc gatactatat
     3001 cctttggcag atacagtaca aacgaatctt tgaagtgctt ttctattaaa tgtactgaag
     3061 gcattattgc ttcctgcaca tgctttgcat aatgtataga gtctacaatc tctttatttt
     3121 taagttgcaa ttgcatagtg cgttggttca ccaactcctg caactcggct tccttatttt
     3181 tgagctgtac aattatttgt ttgctctgct ctcgcattcg tttagtaagc tcgggtataa
     3241 tatgatgtcc aacatttgga tggttttgaa ttaataaata aaacgagtta tgtgtaattt
     3301 cgagtaacga tgaatcggta acggcagtaa cagacatgga acgcacttcg gtatcgagca
     3361 acgagagttc accaaaataa tcgccagcat taagtgtagt tacaacatgt tgttcatcgt
     3421 gtatttgtac ttgcccatta ataacaatat acatggaatt gcctgcgtca cctttattca
     3481 aaacaatttc gtgagccggc acatccttat accgaatatt catagcaaga agttgcaagg
     3541 cctcttgcga gagattgctg aacattgccg tattatgtaa aaaggaaatt acttccgaag
     3601 tttttagcat catctgttag ggaattttta ttccaattac caaaacatcg tcaacctgcg
     3661 agtgctgccc cttccatttt tcgaacaacc gttttaaatg cagctgttgt tgcagcatcg
     3721 ataaatgttg tatagaaaca agtatttctt taaaccgttt ggtcatcaat ttttttccgt
     3781 gttcgccccc aaactgatcg gcataaccat ccgaaaaaat ataaattgta tcgttgggtt
     3841 gtaattgaat atgatgctgc gaaaattttt cttcgtgaat aatttgcatg ccgcctatag
     3901 gaaatttatt gggtttgtaa gtaagcaact ctccatttcg tataagccac atgggtctgt
     3961 tggcaccggc aaaatctaca gtacgtttat ttaaatcaaa ggcaagtatt gaaacatcca
     4021 taccatcgtt agagtcgcgt tgattttgtg gcagcgaata gataattccc tcgttgacag
     4081 caacgagaaa ttcagcaggg ttgg
//
LOCUS       DAORQA010000158         4030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5431_length_4030_cov_8.024906, whole genome shotgun sequence.
ACCESSION   DAORQA010000158 DAORQA010000000
VERSION     DAORQA010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5431_length_4030_cov_8.024906"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1212)
                     /locus_tag="PK736_07385"
     CDS             complement(127..1212)
                     /locus_tag="PK736_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HRC33250.1"
                     /translation="MKNNYCIIMAGGVGSRFWPLSRNAKPKQFLDILGIGKTLLQQTF
                     DRFTKVCPPQNIIVVTNESYVKQVQEQLPQLKADQILSEPHRKNTAPCCAFASYKIMA
                     HNADANIIVAPSDHIIKNEKAFTDVLLSGLKFTAANDALLTIGIKPNRPDTGYGYIQF
                     IDDKKKTKNKISKVKTFTEKPDLEMAKFFLQSGEFLWNAGIFLWSAKSITKALAQYLP
                     EVNDLFEAGMSHYYTEKENAFIKKAYAICPNISIDYGVMEKANNVHVISADIGWSDLG
                     TYGSLYEHMHHDENKNAIVGKNVMAYETKNCVIQMPNNKLVVLQGLDDYIVVESDNVL
                     LVCKKDSEQQIRQFVSDVKINKGESFI"
     gene            complement(1356..2465)
                     /locus_tag="PK736_07390"
     CDS             complement(1356..2465)
                     /locus_tag="PK736_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC33251.1"
                     /translation="MLCDEIVNAYRAGATNYPPSDGLPELRQEVTKFITAREGIEYAP
                     SEIVIACGARPIIYAIFRTVVEKGDKVIFPIPSWNNNHYCHMSEAEKVEIVTTAENNF
                     MPTAEEIRPHLKGATLLSLCSPLNPTGTVFTKEGLAEICDLVLAENASRDADEKPLFV
                     MYDQIYWSITMEGTEHYNPVTLRPAMRNYTVFVDGISKCFAATGLRVGWAIGPAYITH
                     RMRAILSHVGAWAPKAEQIATAKFLANDAEVDIYLHKIRADIKTRLTGLYDGFVRLKS
                     QGMKVDAISPQGAIYLTVQFNLAGCTTPQGNILRNARDVYKYILDEAKVAIVPFYAFG
                     SDDDNSWYRISVGTLPVDQIENIITSIESALQKLK"
     gene            complement(2452..3810)
                     /locus_tag="PK736_07395"
     CDS             complement(2452..3810)
                     /locus_tag="PK736_07395"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HRC33252.1"
                     /translation="MKGSFYGIGAQLKEDDGKIKISSLISGGPAWKGGELKVEDEIIK
                     VAQGNEEPVDVTGYSVIDAVKIIRGSKKGSVVKLTVRRMDGSVIVIPIIRDEIRLDDT
                     FAKSAIINDGDHKIGYIYLPEFYIDFADPNGAKCSEDVANEIKKLKAENVDGIIMDLR
                     SNGGGSLPEVVKMVGLFIPDGPVCLVKGREDKPYQWKDKDPSVLYSGPLTVMVDEFSA
                     SASEIFAAAIQDYKRGIIIGSTSTYGKGTVQRTISLNPDSDNPLFTNRKVEDLGSVKL
                     TLQKFYRINGGATQLRGVTPDIIIPDRLEYLKFREKDNTAALPWDEIGKADYKTWTSN
                     ISNETVVNYIQHQIENDDNIKKIQQGIKWLDENNDKSYSLNLKSYKEDEKVAKEKGKE
                     LDSLFKLRKDLSVKNISADTTAFNMTKDKVEKNTQWLKNLKPHRWRFRPENISNSTVA
                     LR"
     gene            complement(3764..>4030)
                     /locus_tag="PK736_07400"
     CDS             complement(3764..>4030)
                     /locus_tag="PK736_07400"
                     /inference="COORDINATES: protein motif:HMM:NF037004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33253.1"
                     /translation="DDREKNKGKADYKYKADSTLEREAREAVRKQFERFFTTKKTSDN
                     IDENFSTFINAITGSMDPHTDYLAPVDKRYERKFLWYWRTVERR"
BASE COUNT         1048 a          815 c          723 g         1444 t
ORIGIN      
        1 gtattgagat tattagtatt gagattttag tattgagtat tgggactttt agttttgcgt
       61 aactttcgtt ttgaaataga gtgtttgatt cttaattatt aatttctttt ttgcatttca
      121 ttacccttaa ataaaagatt cgcctttgtt tatttttaca tcacttacaa attgccttat
      181 ctgttgctca ctatcttttt tgcaaaccaa gagtacatta tcactttcta caacaatata
      241 atcatcgagc ccttgcaaaa ctacaagctt attgtttggc atttgtatca cacaattttt
      301 tgtttcgtat gccattacat tttttcctac aatggcattt ttattttcat cgtgatgcat
      361 gtgttcgtaa agcgagccat atgtgcccaa gtcgctccaa ccgatatctg cacttattac
      421 atgtacattg tttgcttttt ccatcacacc ataatcgata gagatattgg ggcaaatggc
      481 ataggccttt tttataaatg cgttttcttt ttcggtataa tagtgcgaca taccggcctc
      541 gaacaaatcg tttacttcgg gcaaatattg tgctagagct tttgttattg atttggcgct
      601 ccacaaaaat atgccggcat tccacaaaaa ttcgcccgac tgcaaaaaga attttgccat
      661 ttccaaatca ggcttttcgg taaacgtttt tactttagat attttgtttt tcgttttttt
      721 cttgtcgtca ataaattgta tgtagccata accggtgtct ggccggttcg gttttatgcc
      781 tatcgtaagc aatgcgtcat tggcagcagt aaattttaat cctgacaata acacatcagt
      841 aaatgctttt tcgtttttta taatgtgatc gctgggtgct actataatat tggcatcggc
      901 attgtgtgcc attattttat acgatgcaaa agcacaacag ggtgcagtat ttttacggtg
      961 cggttccgat aaaatctgat cggctttcaa ttgtggcaat tgctcctgta cttgctttac
     1021 atagctttcg tttgtaacaa caataatatt ttgcggtggg cacacttttg taaacctgtc
     1081 gaaagtttgc tgcaataatg ttttgcctat gccaagaata tcgagaaact gtttcggctt
     1141 agcgtttcgg cttagcggcc aaaaccggct gccaacacca cccgccataa taatgcaata
     1201 attatttttc atttattttt tttggtttta cagtgtgtgg ttgtttttgg taaaagtgtt
     1261 tttccacttt cggcacaaaa atattttata aactaccact gtatacgtac ctgttatatt
     1321 tttacaaaca caaaaagtgt ttatagttta aaaagttatt tgagtttttg taatgctgat
     1381 tctatagatg tgataatatt ttcaatttga tcaaccggca atgttcctac cgatattctg
     1441 taccatgaat tatcatcatc ggaaccaaat gcatagaaag gaacgatggc aactttggct
     1501 tcgtcaagaa tgtacttgta cacatcgcgg gcattgcgaa gtatattgcc ttgaggtgta
     1561 gtacagcccg caagattaaa ttgtactgta aggtaaattg caccttgtgg cgatatggca
     1621 tccactttca ttccctggct tttcaatctt acaaaaccat cataaaggcc cgttaatcgt
     1681 gtttttatgt cggctcttat tttgtgcaaa taaatatcca cctcggcatc gttcgctaaa
     1741 aattttgctg tggcaatttg ctccgccttt ggtgcccatg caccaacatg cgaaaggatg
     1801 gcacgcatac gatgtgtgat atatgccggc cctattgccc aacctacgcg aagaccggtt
     1861 gccgcaaagc acttcgaaat gccatcaaca aacacagtat aattgcgcat tgccgggcga
     1921 agcgtaacag gattgtaatg ctcggtccct tccatagtaa tagaccagta tatttgatcg
     1981 tacataacaa agaggggttt ttcgtcagca tcacgcgatg cattttcggc caacaccaaa
     2041 tcgcatattt cggcaaggcc ttcttttgta aatacggtgc cggtgggatt gaggggcgag
     2101 caaagtgata atagtgttgc gccttttaaa tgcgggcgaa tttcttcggc cgtgggcata
     2161 aagttatttt cggctgtagt tacaatttct actttttcgg cttcgctcat gtggcaatag
     2221 tgattgttgt tccacgatgg tatcggaaaa ataaccttgt cgcctttttc aaccacggtg
     2281 cggaaaatgg cgtaaataat ggggcgtgca ccacaggcta ttactatttc ggatggggca
     2341 tattctatac cttcgcgagc agtaataaat tttgttactt cttgcctcaa ctcgggaagt
     2401 ccatcgcttg gtgggtagtt ggtggcaccg gcacggtatg cgtttactat ttcatcgcaa
     2461 agcaactgtg gaattggaaa tattttcggg tcgaaatcgc caacggtgag gttttaaatt
     2521 ttttagccat tgagtatttt tttctacttt atcttttgtc atattaaagg cagtcgtatc
     2581 agcactaata ttttttacag aaagatcttt ccttagttta aataggctgt ccagttcttt
     2641 acctttttct tttgccactt tttcatcctc tttataactt tttaaattta aagaatagga
     2701 tttatcatta ttttcatcta gccactttat gccttgctgt atctttttta tattatcatc
     2761 attctctatc tggtgttgaa tataattaac aacggtttcg ttactgatgt tactcgtcca
     2821 ggttttataa tcagctttgc caatctcatc ccagggtagc gctgctgtat tatctttttc
     2881 tctgaatttt aaatactcaa gcctgtcggg gataataata tcaggggtta ctcctcttaa
     2941 ttgtgtagct cctccattta tacggtaaaa tttttgcaag gttagtttaa cagatcccag
     3001 gtcttctact ttacggttag taaataatgg attatcgcta tcaggattta atgaaatggt
     3061 tctttgtact gttccttttc cataagtaga ggtactacca atgattatac cccttttgta
     3121 atcctgaatg gcagcagcaa aaatttcaga agcactggcg ctgaattcgt caaccataac
     3181 ggttaagggt ccgctgtata aaacacttgg atccttatct ttccactggt aaggtttatc
     3241 ttccctacct tttacaagac aaaccgggcc atcgggaata aacaagccta ccattttaac
     3301 cacttcagga agtgagcctc caccattgct tcgtaaatcc ataatgatgc catctacgtt
     3361 ttctgctttt aattttttta tttcattggc cacatcttcc gagcatttgg caccattggg
     3421 atcggcaaaa tcaatataaa actcaggtaa atatatatag cctattttat gatcgccatc
     3481 attaatgatg gcactttttg cgaatgtatc atcaagcctt atttcatccc taattattgg
     3541 aattactatt acagaaccat ccatcctgcg tacggtaagt tttactacag agcctttttt
     3601 cgatcccctg attattttaa cagcatctat tacactatat ccagttacat ctacaggttc
     3661 ttcattgcct tgtgctactt taatgatttc gtcttccact tttagttcgc ctcctttcca
     3721 tgcagggccg cctgaaatta gtgaagatat ttttatcttt ccgtcatctt ctttcaactg
     3781 tgcgccaata ccataaaaac ttcctttcat atcttttatc taccggggct aaataatccg
     3841 tatgaggatc catcgaacca gtaatggcat ttataaaagt gctgaaattt tcatctatgt
     3901 tatcacttgt ttttttagta gtaaagaatc tctcaaattg cttgcgcacg gcttctcttg
     3961 cttctctttc taatgtactg tcggctttat atttataatc agccttacct ttgtttttct
     4021 ccctgtcatc
//
LOCUS       DAORQA010000159         4022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5443_length_4022_cov_9.156037, whole genome shotgun sequence.
ACCESSION   DAORQA010000159 DAORQA010000000
VERSION     DAORQA010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5443_length_4022_cov_9.156037"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            581..973
                     /locus_tag="PK736_07405"
     CDS             581..973
                     /locus_tag="PK736_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRC33254.1"
                     /translation="MNIVHASIGTNKYKTTLRTSRNSIIADEPLSLGGSDLGFSPTQL
                     LCSSLAACTSITLRMYANHKNWNVVDIFVTVEFDKNNETGISTFTRKIEIKGAITDEQ
                     KQRLLDVANKCPVHKILSNPIQINTTIG"
     gene            973..1434
                     /locus_tag="PK736_07410"
     CDS             973..1434
                     /locus_tag="PK736_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33255.1"
                     /translation="MGILKISKEIIFATCEQKLHEKINYLQKEMSDLMYGLENDTKSS
                     AGDKHETARAMMQIDYEKSLRQLTDANTQMSLLKKINHHTDANVISAGSLIYTNHGAI
                     FIAISLGKIKIEQHDVMVISPQSPLALKFMQAKKSSSISMNGIDYIIEKVE"
     gene            complement(1452..2732)
                     /locus_tag="PK736_07415"
     CDS             complement(1452..2732)
                     /locus_tag="PK736_07415"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRC33256.1"
                     /translation="MEKVIKIVCLILGGGAGSRLYPLTAIRSKPAVPIAGKYRLIDIP
                     ISNCLHSGIKRIFVLTQYNSASLNQHIKNTYHFDHFSYAFVDILAAEQRPGSEKWFQG
                     TSDAVKQSLPHLKGHDYDYILILSGDQLYQMDLREFVNKHVESGAEISIATLPVDKDD
                     APDFGIMKVNENGTINKFIEKPSRDELVNWISPVGAKQKAEGKEYLASMGIYVFNKET
                     LSRLFDQNPHFNDFGKEIIPGAINQGIKVNGYSYTGYWTDIGTIKSYFEANLDLAELI
                     PQFNLFDNEKLIYTRARMLPPSKFSSNTILKGSIISEGCIINAQLIERCIIGNRARIG
                     EGTVMKNCITFGNDFYETLHELTNSEKGFNIGIGRNCHFENCIIDKDVKIGDNVTIIG
                     NINMPDTETESYCIKDGIVIIKKDAQIGDNVNLS"
     gene            complement(2738..3784)
                     /locus_tag="PK736_07420"
     CDS             complement(2738..3784)
                     /locus_tag="PK736_07420"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRC33257.1"
                     /translation="MKNWSDKPEVLHCHDHHTGLIPFMIKHCPAYSLLAHVRTVFTIH
                     NGLYTGAFAWYNSRFFPEFSFQSKGSLDWDDTINPLACAIKCCDWLTTVSGGYLDELR
                     FQPNPLQSLYNQHWQKSKGIVNGIDADVWNPKTDKNLTVKLAKSILKFKQKSKQEILK
                     DTNLNPDLPLIVFIGRLVNEKGGELLPQAIGRYLSRVGNVNFYVLGSGSSDIENQLQR
                     VRELFTQSVATYIGYNEALAHKLYAGADFLIMPSIIEPCGLNQLYAMRYATVPMVRSV
                     GGLKDTVVDIGDTNGYGIRFTNANVEDILYSVNRAVDLYANPKLLEEIRKRLVALNFS
                     WEKAVKEYIEIYQY"
     gene            complement(3790..>4022)
                     /locus_tag="PK736_07425"
     CDS             complement(3790..>4022)
                     /locus_tag="PK736_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HRC33258.1"
                     /translation="VLLPQYNQKWVQQNTKVNVWKGNLSSSHFNFNYYINQCVDAGLG
                     FEVYLVDIPDLLFRENIYGYQDDHLRFFNRLR"
BASE COUNT         1325 a          794 c          703 g         1200 t
ORIGIN      
        1 actgcaatgt ggcttacgca tcaatcattc cttggaaaag ggatttataa tttaattttt
       61 gtgtttagct aaccagtaaa taaatattcg gctttagcca aataaaatac atcaaatacg
      121 ctgcgtcaaa atagtttcat tgccgttggc tttagccaac ggattaaaaa tattcagctt
      181 tagcctaaaa gaaaatcaag cacacactct gcgtcaaaat ggattcattg ccattggctt
      241 tagccaacgg attaaaaata ttctgattta gccgaaaaga aaatcaaccg caaaccctgc
      301 gtcaaaattg tctcattgcc gttggcttca gccaacggat taaaaatttt aagccttagc
      361 ctaaaagaaa tcaagcacaa accctgcgtc aaaatagttc cattgtcgat ggctttaacc
      421 aacggattaa aaatattcag ctttagccta aagaaaatca agcataaacc ctgcgtcaaa
      481 atagtttcat tcccgttggt ttagccaacg gattaaaaac agcaattcca tcaaccttcc
      541 aaaatgtttc ataaatttga ggcatctaat ttctaatatt atgaatatag tacatgccag
      601 tattggtacc aacaaataca aaactacatt acgcacaagt cgcaatagca taattgccga
      661 tgagcctttg agtctgggtg gtagcgattt aggattttcg ccaacacaac ttttgtgtag
      721 ttcgctggca gcatgcacaa gtatcacatt gcgtatgtat gcaaaccata aaaactggaa
      781 tgtggttgat atatttgtaa ctgtggaatt tgacaaaaat aatgaaacgg gtatttcaac
      841 ttttacaaga aaaattgaaa taaaaggcgc aataacagac gaacaaaaac aacgcttact
      901 tgatgtggcc aacaaatgcc cagtacataa aatattgagt aaccctattc agataaatac
      961 aactataggg tgatgggaat tttaaaaatt tcgaaagaaa taatatttgc aacatgcgaa
     1021 caaaagttgc atgaaaaaat aaattacctg caaaaggaaa tgagcgattt gatgtatggt
     1081 ttagaaaacg acaccaaaag ttcggcaggc gataagcatg aaacggctag ggcaatgatg
     1141 caaatagatt acgaaaaaag tttgcggcaa ttaaccgatg ctaatactca aatgtcatta
     1201 ttgaaaaaga taaaccatca taccgatgcg aatgttattt cggcaggcag tttgatttat
     1261 acaaatcacg gagcaatttt tatagcaata tcattgggca aaataaaaat tgaacaacat
     1321 gatgtgatgg ttatttcacc tcaatcgcca ctggcattaa aatttatgca agcaaaaaaa
     1381 tcatcatcaa tatcaatgaa tggtattgat tatattattg aaaaagttga gtaacttaag
     1441 gcataaatat ttcaactcaa atttacatta tcaccaattt gtgcatcttt cttaatgata
     1501 actattccat cttttatgca atagctttct gtttcggtat cgggcatatt gatattacca
     1561 ataatggtaa cgttgtcacc aatttttaca tccttatcaa tgatacaatt ttcgaaatga
     1621 caattgcggc caataccaat attaaaacct ttttcgctat tggttaactc gtgcaaagtc
     1681 tcgtaaaagt cgttaccaaa ggtgatacaa tttttcatca ctgtgccctc ccctattcgc
     1741 gcacgattac ctataatgca gcgttcaatt aattgtgcat tgataataca accttccgaa
     1801 ataatggaac ccttcaaaat agtattgctc gaaaatttgg atggtggcaa catgcgtgca
     1861 cgtgtgtaaa tcaatttttc gttatcgaac aaattgaact gcggaattaa ttctgccaaa
     1921 tcgaggttgg cttcaaaata actttttata gttccaatgt cggtccagta gcctgtatac
     1981 gaatagccgt tcacctttat gccctggtta atagcaccgg gaattatctc cttaccaaaa
     2041 tcgttgaaat gtggattttg atcgaagagt cgcgacaaag tttctttatt gaagacgtat
     2101 atgcccatag aagcaagata ctccttaccc tctgcttttt gtttagcacc aacaggcgaa
     2161 atccaattta ccaattcatc gcgcgatggc ttttcgataa atttattaat agtaccattt
     2221 tcattcactt tcattattcc aaaatcgggt gcatcgtctt tatcaacagg caaagtggct
     2281 atgctaattt cggcaccact ttcaacatgc ttgtttacaa actcgcgtaa gtccatttga
     2341 taaagttgat cgccacttag tataagtata taatcgtagt cgtgcccctt aaggtggggc
     2401 aacgattgtt tcacagcatc ggacgtaccc tgaaaccatt tctcgctgcc gggtcgttgt
     2461 tcggcagcaa gtatatcgac aaatgcataa ctgaaatgat cgaagtggta tgtgtttttt
     2521 atgtgctgat tgagtgaggc cgaattatat tgtgtaagaa caaatatgcg cttgatgccc
     2581 gagtgcaaac aattagaaat gggaatatca atcaacctgt acttgccggc tataggcaca
     2641 gcaggcttgc ttctgatagc ggtgagagga tacagacggc ttccggcacc tccacctaaa
     2701 atgagacata ctattttaat tactttttcc atatcgttta atattgataa atttctatat
     2761 attcttttac tgccttttcc caagaaaaat tcaatgccac cagccgcttg cgaatttcct
     2821 ctaatagttt tggattggca tacaaatcca ctgcgcggtt cacactgtat aaaatatcct
     2881 caacattcgc attggtaaat cgtataccgt aaccgttagt atcaccaata tcaactacag
     2941 tatctttcaa tccaccaacg gaacgcacca ttggcacagt ggcatatcgc attgcatata
     3001 attgattgag accacagggt tcaataattg atggcattat caaaaaatcg gcaccggcat
     3061 ataacttatg tgcgagtgct tcattgtaac caatataggt agctacgctt tgagtgaaca
     3121 actcccttac acgttgcaat tgattttcta tgtcggacga acccgaaccc aacacataaa
     3181 aattaacatt acccacacgt gataaatatc gtcctatagc ctgaggcaga agttcgcctc
     3241 ccttttcatt tacaagtctg cctatgaata ctattaaagg taaatcggga ttgagatttg
     3301 tgtcttttaa tatttcctgc ttactttttt gtttgaattt gaggatgctt tttgcaagtt
     3361 ttacggtcaa gtttttatcc gtttttggat tccatacatc agcatcaata ccatttacaa
     3421 ttcctttgct tttttgccaa tgttgattgt acaatgattg aagaggattg ggctgaaaac
     3481 gcaactcatc taaataacct ccacttactg tagttaacca atcgcaacat ttaatggcgc
     3541 atgcaagcgg attaatggta tcgtcccaat cgagggaacc cttcgactgg aaagaaaatt
     3601 ccggaaaaaa gcgtgaatta taccaggcaa atgcacccgt gtataaacca ttatgtatag
     3661 taaaaacagt gcgcacatgg gcaagcaaac tataagcagg gcaatgcttt atcataaaag
     3721 gaataaggcc tgtatgatga tcgtgacaat gcaaaacctc gggtttatct gaccagtttt
     3781 taatccaatc taacgcagcc tgttgaaaaa cctcaaatga tcatcctggt agccataaat
     3841 attttcgcga aagagcaaat cgggaatatc aacaagataa acttcaaagc ccaaaccggc
     3901 atctacacac tgatttatgt aataattaaa attgaaatgt gaactactaa gatttccttt
     3961 ccacacattc accttggtgt tttgctgcac ccacttttgg ttatattgag gtaataacac
     4021 ac
//
LOCUS       DAORQA010000160         4015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5452_length_4015_cov_8.720960, whole genome shotgun sequence.
ACCESSION   DAORQA010000160 DAORQA010000000
VERSION     DAORQA010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5452_length_4015_cov_8.720960"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..3891
                     /locus_tag="PK736_07430"
     CDS             133..3891
                     /locus_tag="PK736_07430"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HRC33259.1"
                     /translation="MKKHLLMFLCISAFVLNVTKTKAQATVQIVHNCGDTLLAQVDVY
                     VNGALALNDFQYRTATQFISLPSGVALNIGVAFSNSVSANDTVRNYQVTFTAGERYVV
                     FVNGVFHVAKYAPNPNNQGTGMKLEIVSGVRATALNTGEVDLLAFNGCTELQEIDFRV
                     RPNILAGDNLKFRDFSSYFSLNAAKQIIDLNPGAQAQNIIYSHVADLSTYANATGVVF
                     TSGFLNPALNENGKPFGQFIVFNNGTVIELSKLKTARLQVIHNSADSLLENVDVWING
                     DKTINNMKFRDATMYMDIPAEYPLNVGIADNSSSVVTDTLRNFEFILDSGKTYILIAQ
                     GVVNTSLFAPNPDGKNIDITLLPLPDAREQSSTAGNVEFMLVHGSTDYETVDMHVRNG
                     AQIGDNLSYGDNSAYASLPAANYILDMKDAAGAGIIASYQANLANFADSTAVIFSSAH
                     KNPQSNWNGKPFAWYIAYNSGYVIKLQEAATSRLQVIHNCADPAADAVDIYVDGTLTY
                     DDLAFRAATAFADVAPGIHQIGIAPKTSASVNDTIKNYTIDFKNGKTYIAIAMGVITP
                     ANFAVNPDAIAIDFMVNVKDTYRETANNAGNVDFVVAHGCTDAPTVDVIARNVATVAN
                     NAAYSDITGYNSLAANNYIFDVTDANNANVLFSYQANLTSLAGKSAVVFASGFINPAA
                     NQNGAAFGLFAALPDGNVIPFTPITSARLQLVHNAADPAAALVDVYVNGTLAYDDFAF
                     RNATPFSDIAAGVLTNIGIAPSTSTSVNDTIKSIDVTFDIAKTYIVFVNGVTNAANFV
                     ANPDGKNISLNAYAINDGRETGSNPAKFDVNVFHGVTDAPTIDVVVRNGGVLVDNAAY
                     GDFTGYVSVDTANYILDVTPGSNNNTILKSYRADLLPLGGQSGVVFASGFLDSLAANQ
                     TGAPFGLFMVFPAGYVIPLTEASEARIQVIHNCADPAADSVDVYVNGFLLVDNFPFRT
                     ATPFISIGAGVTYNVGIAPKNSTSANDTIRNFEAALENGKTYIAIASGVISPASFAVN
                     PDGKATDFNVWLTEGVRETSALAGNFDFITINGITDAPTVDLIARNVMTLNDNIAYSD
                     ISPYSTLPGGQYLFDVTDATNTTTFSTYNADLTSLIGKAGVVFGSGFVTPANNQNGVL
                     YGLWVAMPDGSTFPLPIVTAINEAFDNGTFAMYPNPAIDNLTITLNNNQKNVRVQVLN
                     NVGQIVLTQTNNFTTNAMSINIAKLSAGVYTVQLISENDIVSTKFVKQ"
BASE COUNT         1142 a          786 c          801 g         1286 t
ORIGIN      
        1 attgatgttt tctattttct ttttcgaaaa gatgcatcat ttttgcaagc aactattttt
       61 atcaataaac ctattattta caatggtttt tgacaatgaa tcgaaatcga aaattttaat
      121 tttatctata ttatgaaaaa acatttatta atgttcttat gtatcagtgc ttttgtgctg
      181 aatgttacca aaactaaagc acaagccacc gtgcagattg tacacaattg tggcgatact
      241 ttgcttgctc aggtagatgt gtatgtaaat ggcgctttgg cacttaacga ttttcaatac
      301 cgcactgcca cacagtttat aagcttgcct tcgggtgttg ctttaaatat tggtgtggca
      361 ttttcaaata gtgtttccgc caacgataca gtgcgcaact atcaggtgac atttactgcc
      421 ggtgagcgtt atgtggtatt tgtaaacggg gtatttcatg ttgcaaaata tgctcctaat
      481 ccaaacaatc aaggcacagg tatgaaactc gaaatagtaa gtggtgtgcg tgcaactgca
      541 ttgaacacag gtgaagttga cttgcttgca tttaatggtt gcactgaatt gcaagaaatt
      601 gactttcgtg taaggccaaa tattttggcg ggcgacaatt taaagtttcg cgatttttct
      661 tcttactttt ctttgaatgc tgccaaacaa attatagact taaatcccgg tgcgcaggca
      721 cagaacatta tttactcaca tgttgccgat ttgtctacgt atgccaatgc taccggagtt
      781 gtttttacct caggattttt gaaccctgca cttaacgaaa acggaaaacc ttttggtcag
      841 tttatagtgt ttaataacgg tactgttatc gagttatcaa aattgaaaac agcccgttta
      901 caggttattc ataacagtgc cgacagcttg ctcgaaaatg ttgatgtttg gataaatggc
      961 gataaaacaa tcaataatat gaagtttcgt gatgctacta tgtacatgga tatacctgct
     1021 gaataccctt tgaatgttgg cattgccgat aattcaagtt ctgtagtaac cgatacactg
     1081 cgcaactttg agtttatact cgatagcgga aaaacgtata ttcttatagc acagggcgta
     1141 gttaatacca gtttgtttgc tccaaatcct gatggaaaaa atatcgacat tacattattg
     1201 ccattgcccg atgcacgcga gcaatcgtca acagcaggca atgtagagtt tatgcttgtt
     1261 catggcagca ctgattacga aacggtggat atgcatgtcc gtaacggagc acaaattggc
     1321 gataatttat cttatggtga taactctgca tatgcatcgt tgccggccgc aaattatatt
     1381 ttggatatga aagatgcagc cggtgctggc attattgctt cttatcaggc caatctcgct
     1441 aactttgccg atagcactgc agttatcttt tcttcggcac acaaaaatcc acagtccaat
     1501 tggaatggaa aaccttttgc ctggtatatt gcatacaact cgggttatgt gataaagctt
     1561 caggaggcgg caacatcacg ccttcaggtt atacacaatt gtgccgaccc tgctgccgat
     1621 gcggtagata tttatgttga tggaacactc acctacgatg acctagcatt tcgcgcagct
     1681 actgcctttg ccgatgttgc tccaggaatt catcagattg gtattgcccc taaaacaagt
     1741 gcttcggtta acgatactat taagaattat accatcgatt ttaaaaatgg caaaacctat
     1801 atcgccattg caatgggtgt aattactccc gcaaattttg ctgtcaatcc cgatgctatt
     1861 gccatcgact ttatggtaaa tgtaaaagat acttatcgcg aaacagcaaa caatgctggc
     1921 aacgtagatt ttgttgtagc tcatggctgt accgatgcac ccacagtgga tgttattgcc
     1981 cgcaatgttg caacggttgc aaacaatgca gcatattccg atattaccgg atacaattcg
     2041 cttgcagcca acaattatat ttttgatgtt accgatgcca acaatgcaaa tgttttattt
     2101 tcttatcagg caaatcttac ttcgctagcc ggcaaatcgg ctgttgtatt tgcaagtggt
     2161 tttattaatc ctgctgccaa tcaaaatggt gctgcgtttg gtttatttgc tgccttgccc
     2221 gatggtaatg ttattccatt tactcctata acaagtgcac gtttgcaatt ggtgcataat
     2281 gctgccgacc ctgctgctgc gcttgtcgat gtatatgtga atggcacttt ggcttacgat
     2341 gattttgctt tccgcaatgc tactccattt agcgatatag ctgctggcgt gctcaccaac
     2401 attggtatcg ctccatcaac aagtacatct gtaaacgata ctatcaagtc tattgatgtt
     2461 actttcgaca ttgccaaaac gtatatagtg tttgttaatg gtgttaccaa tgcagcaaat
     2521 tttgttgcca accccgatgg aaaaaatatt tctttaaatg cttatgcaat aaacgatggt
     2581 cgcgaaacag gttctaaccc tgccaagttt gatgtaaatg tatttcatgg tgttaccgat
     2641 gcacctacta tagatgtagt tgtacgtaat ggcggagtgt tggttgataa tgctgcctat
     2701 ggcgacttta caggttatgt ttctgttgat acggcaaatt atattttgga tgtaactccg
     2761 ggcagcaaca acaatacaat attgaaatcg tatcgtgccg atttgttgcc acttggcgga
     2821 caaagtggag ttgtgtttgc ttccggattt ttagattcac tggctgccaa tcaaacaggc
     2881 gctccatttg gtttattcat ggtatttcct gcgggatatg ttattccttt aacagaagca
     2941 tcggaagcgc gcatacaagt aatacacaat tgtgccgacc ctgctgccga ttcggttgat
     3001 gtatacgtta atggattttt acttgtagat aatttcccat tccgcactgc cacaccattc
     3061 atttcgattg gtgcaggtgt aacttataat gtaggtatag cccctaaaaa cagtacaagt
     3121 gccaacgata ctattcgcaa ttttgaggct gcattagaaa atggtaaaac atatatcgcc
     3181 atagcttcag gtgttatttc tcctgcttcg tttgcagtga accccgatgg aaaagctaca
     3241 gattttaatg tgtggttaac cgagggtgtg cgcgaaactt ctgctcttgc cggcaatttc
     3301 gatttcatta ccattaatgg aattaccgat gcacctactg ttgatttaat tgcacgcaac
     3361 gtaatgacct tgaatgataa tattgcttac agcgatattt cgccctattc aactttaccc
     3421 ggtgggcaat atttatttga tgttaccgat gctacgaata caacaacgtt ttcaacttac
     3481 aatgctgact taacatcact cattggaaaa gccggagttg ttttcggttc gggatttgtt
     3541 acaccagcca ataaccaaaa tggtgtgctg tatggcctgt gggttgcaat gcccgatggt
     3601 agcaccttcc cattaccaat tgttactgca ataaacgagg cattcgataa tggtactttt
     3661 gcaatgtatc caaatccggc catcgataat ttgacaatca cattaaataa taatcaaaaa
     3721 aatgtgcgag tgcaagtatt gaacaacgtg ggccaaatag ttttaacaca aacaaataat
     3781 ttcaccacaa atgccatgtc aataaacatt gcaaaattat ctgctggtgt gtatacagtt
     3841 caattgataa gtgaaaatga tattgtatcg acaaaatttg tgaaacaata atttggaaaa
     3901 taattttaag aactataaag aagctgctca attgggcagc tttttttatg ggttttattt
     3961 ttttattcgg cattcattcg ttccattcat ggcatataaa attaaccata taagg
//
LOCUS       DAORQA010000161         3988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5493_length_3988_cov_10.810323, whole genome shotgun sequence.
ACCESSION   DAORQA010000161 DAORQA010000000
VERSION     DAORQA010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5493_length_3988_cov_10.810323"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PK736_07435"
     CDS             complement(<1..408)
                     /locus_tag="PK736_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33260.1"
                     /translation="MKSQGYDNLWVHGFLSGYPNFGGTKMNYINKTLDTSFFDKKVNF
                     RLTVGVICNKQGDLEFYSNGITVANAQNDTMPNGDSLGTSALMIKLYQDGLMYIQGML
                     VIPFPIDTTKYYLFHEGGDSTNLFGGGYFPTSLL"
     gene            complement(473..>3988)
                     /locus_tag="PK736_07440"
     CDS             complement(473..>3988)
                     /locus_tag="PK736_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33261.1"
                     /translation="PAANYCTGAPISIVVDNPQNFINYVWSSGCTQGSPHVAQVNPTS
                     TPTTYTVTVTDAGGFTHTTTIVVQGTQNNLSFNIPATTYNTCANTPIQITATLQNGQA
                     ANFASYALYADQSADPGFANKQSDGYKFISNGSQFTQNVTPKKTTDYYIMASDGQCAA
                     MSQKITVNVAPYADLGPDKEFCTSGTGIILNPYEEQIPPANVTYNWSTGSTSPTLSVA
                     TAGTYTVTVTDLTNTCTTTDNIVLTPISCCNNSLTNEIIPPNTIIDKGYTNANLQITT
                     TNGLTVISPKVLPAKQNLEIQGTIHIMDNVRLQNFATINCSEAAQIDVRPYSRFGIFD
                     SYVKSCTNYLWKGIDVKRFGDLVIKSVNKLCKITEAEFGVTIRKDCTIEIEDIDFVDN
                     FVGLFVPPTINFELANDVDITITGSRFYCDNNLKLAYGGLSPQISNYVPKTVSLAGMV
                     LNQTASSIGVSLQNYNQFGNSNTSKPKMNYGIIAYNSALNVQATMFQNIADIDKYGKL
                     GGTSIYSSNQYGYGSLIETGLGKQNSTPTISNSYRSIYIDHGTATIKQNFISSCDFGP
                     TLYYSPGGNSLFESNKIITRIASLWLSYNFNANLVETKLNQYLNANYVPNNIISAGIV
                     VLEPDKFTKLIMHESQYILGGAYTKYGMYISMVHGAVIGCHDIDLSLTLQTNMSLGMY
                     VARGGDNDLRRITIKGNGNYSYCNTEGMHFLSSTKNIIRCNSTNNNKYGFVFDGVCTM
                     PERFKTNEIKNAYEGLHVSSEGLMDNQTNTGNTWTGSFTNYGANNERINKYLSTTDVV
                     KYSISGTNNLPLLPANQIGWFVQSTASDATCDTASCVYIKPIIGDGETATDIAIASGT
                     IGFPQFEEEMIHAAEQQEAENLSLNQDSIDNDTLLTFLNNYRNSNNGIFQLINDTILN
                     IVDNNKIQFAFNDSIVRYNLQIGAVYDSIIVDSLSSSLTISNARIIKLKLLNEVNQLL
                     HQNQLYSQQYQNNLGSRINIALQMNNAANTFGKTYISNEKVANEMFLQSLIQQDSDYT
                     SAQWQTLKSIAAQCPHTGGKGVIKARNLILSKCDSIFWDDAPTCALEGYRRKAKKEGV
                     DDIAILSPNPAKYYTDLLYKTNDDIALTLKVLDNYGQVILVKQLNQNQNTDRINTSLF
                     PNGCYQIELFSGERKVSCQKLIILK"
BASE COUNT         1178 a          717 c          708 g         1385 t
ORIGIN      
        1 tagcaaagaa gttggaaaat aacctccacc aaataaattt gtagaatcac cgccctcatg
       61 gaataaataa tattttgtag tgtcaattgg aaatggtatt accaacattc cttgaatata
      121 cataagacca tcttgatata atttaatcat aagtgcactt gtaccaagac tatcgccatt
      181 aggcattgta tcgttttgag cgttggcaac cgtaatgcca ttgctgtaaa attccaaatc
      241 gccttgttta ttgcaaatta caccaacagt caatctaaaa ttaacttttt tatcaaaaaa
      301 acttgtatca agtgtcttat tgatgtaatt cattttggta cccccaaaat ttggataacc
      361 actcaaaaaa ccatgaaccc acaaattgtc gtatccttga cttttcattt taaaatttga
      421 caaaaccaca atgagaataa aaaaaacaag tttcgttttc attgccaaaa aattacttga
      481 ggataattaa tttttgacaa cttacttttc gttcacctga gaataattct atttggtagc
      541 atccgttagg aaataaactc gtattaattc tatcagtatt ttggttttgg ttcaactgtt
      601 ttactaaaat aacttgacca taattatcca gcacttttaa agttaaagca atatcatcgt
      661 tggttttgta caacaaatcg gtataatatt ttgccggatt tggcgataat attgctatgt
      721 catcaactcc ttctttttta gcttttcgcc gatatccttc gagcgcacag gtgggtgcat
      781 cgtcccaaaa aatgctatcg catttgctca atattaaatt gcgcgccttt attactcctt
      841 tgcctcctgt gtgtgggcat tgtgctgcaa tactttttaa tgtttgccat tgtgccgaag
      901 tataatcgct gtcttgctgt attaatgatt gcaaaaacat ttcgttcgca actttttcgt
      961 tcgatatata tgttttgcca aaagtgtttg ccgcattgtt catttgtaaa gcaatgttta
     1021 ttctgctacc taaattgttt tgatattgtt ggctatacaa ctgattttgg tggagtagtt
     1081 gatttacttc attcaacaat ttcagtttaa ttattctggc atttgaaatt gttaatgacg
     1141 atgataatga atcaacaata atgctatcat acactgcacc tatttgcaaa ttataccgca
     1201 caatactatc attaaaagca aattggattt tattattgtc aactatgttt aatatcgtat
     1261 cgttaatcaa ttgaaaaatg ccgttattag aattacggta attatttaga aaagtaagta
     1321 aggtatcgtt atcaattgaa tcctggttca ggctcaaatt ttctgcttct tgttgctctg
     1381 cagcatgtat catttcttct tcaaattgtg gaaaaccaat tgtgcccgaa gcaattgcaa
     1441 tgtcggtggc agtttcgcca tcaccaatta ttggttttat gtatacacaa cttgccgtgt
     1501 cacaggttgc atcggaagcc gtagattgaa caaaccaccc aatttgattt gcaggcaata
     1561 acggcaaatt atttgtacca ctaatgctat atttaaccac gtcagtagta ctcaggtact
     1621 tattaatcct ctcattattg gcaccgtaat tagtaaagga gcctgtccaa gtatttcctg
     1681 tattagtttg gttgtccatt aggccttcgc tcgatacatg caagccctca taagcatttt
     1741 ttatttcatt ggttttaaat cgttcgggca tggtgcatac gccatcaaat acaaaaccgt
     1801 atttattgtt gttggtggag ttgcagcgta taatattttt agtgctgcta agaaagtgca
     1861 tgccttctgt attgcaataa gaatagttgc catttccttt tatggttatt cgcctgaggt
     1921 cgttgtcgcc accacgtgcc acatacatac ccaaactcat gttggtttgt agtgttagtg
     1981 ataaatcaat atcgtggcaa ccaattacag caccgtgtac catacttata tacataccgt
     2041 attttgtata tgcacctcct aaaatgtatt gactttcgtg cattatcagc tttgtaaact
     2101 tatctggttc aagtacaact atacctgcac ttattatatt attgggtaca tagttagcgt
     2161 ttaaatattg attcaatttg gtttcaacca agtttgcatt aaaattgtaa cttaaccaaa
     2221 gtgatgctat tcgagttata attttgtttg attcgaataa agaattacca cctggcgagt
     2281 aatatagtgt tgggccgaaa tcgcaggaac tgataaagtt ttgttttatg gtagctgtac
     2341 catggtcaat atagatagag cggtagctat tgctaattgt aggtgtagaa ttttgtttgc
     2401 ccaatcccgt ttctatcaac gagccatatc catattgatt ggaagaataa atagaagtgc
     2461 cccccaattt accatactta tcaatatcag caatattttg aaacatggta gcttgaacat
     2521 tcaatgctga attataagct ataataccat aattcatttt tggtttgctt gtattgctat
     2581 taccaaactg attatagttt tgcaaagaaa caccaattga ggaagctgtt tgatttagaa
     2641 ccatgcccgc caaagacaca gttttaggaa cataattaga aatttgagga cttagtcctc
     2701 cgtatgccaa ttttaaatta ttatcgcaat aaaacctgct accggttata gtaatatcta
     2761 catcattggc taattcaaaa ttgatggttg gcggtacaaa caatccaaca aaattatcga
     2821 caaaatcaat gtcctctatt tcaatagtac aatcttttct aatcgtaaca ccaaattctg
     2881 cttcagtgat tttacatagt ttgttcacag actttataac taaatcacca aaacgcttta
     2941 catcaatgcc tttccataaa taatttgtgc aactttttac atacgaatcg aaaatcccaa
     3001 aacgtgagta aggcctcaca tcaatttgtg cagcctcact gcaatttatg gttgcaaaat
     3061 tttgcaggcg cacattatcc ataatatgta tggttccctg aatttctaaa ttttgtttgg
     3121 caggaagcac tttgggtgat attactgtta atccatttgt tgtagtaatt tgaagattgg
     3181 catttgtata tcccttatca attattgtat taggtggtat tatctcattg gtaagtgaat
     3241 tattgcaaca acttataggc gtaagcacaa tattgtcggt agtggtgcaa gtattggtta
     3301 aatcggtaac ggttacagta tacgtacctg cggtagctac acttaatgta ggtgaagttg
     3361 agccggtact ccaattataa gtaacatttg caggtggtat ttgctcctca tacggattga
     3421 ggataatgcc tgttccagag gtacaaaatt ctttatcggg gcctaagtca gcatagggtg
     3481 ccacgtttac ggttattttt tggctcatgg ctgcacattg cccatcactt gccattatgt
     3541 aataatctgt agtttttttt ggggtcacat tttgagtaaa ttggctgcca ttacttataa
     3601 atttgtagcc atcgctttgt ttgttggcaa aaccggggtc ggcagattgg tctgcataca
     3661 gcgcataaga agcgaaattt gcagcttgtc cgttctgaag ggtagctgtt atttgtatcg
     3721 gtgtatttgc acaagtatta tatgtagtgg caggtatatt aaatgataaa ttattttgag
     3781 ttccttgtac aacaattgtt gtcgtatggg taaatccgcc agcatcagta actgtaaccg
     3841 tatacgtagt tggtgtgctt gttgggttta cttgcgccac atgagggcta ccttgtgtgc
     3901 aaccacttga ccacacataa ttgataaaat tttgcggatt atcaaccaca atagaaatag
     3961 gtgcgcctgt gcaatagttt gcggcagg
//
LOCUS       DAORQA010000162         3970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5524_length_3970_cov_17.757854, whole genome shotgun sequence.
ACCESSION   DAORQA010000162 DAORQA010000000
VERSION     DAORQA010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5524_length_3970_cov_17.757854"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="PK736_07445"
     CDS             <1..1253
                     /locus_tag="PK736_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33262.1"
                     /translation="SGFFYSRRVHVLNFQLAFLLKLAAALVMTFIYTYFYPDRHVADI
                     FRYFDDSNVLIQMFENNVSDFFAFITGHDNAALYKYKITMNNWESPGNVYNNNRVMII
                     LNAAIGFISLGDYYVHLILFTFLSFVGLTAMFKVFNKIYKVYQGIVFISVFTLPSIIF
                     WTSGVLKESLVMFALGIFLYSYMRFYFNSAIWYHLFSFALGFIILMLLKFYLLIILLP
                     AMIILLLTQRILILQKMKGLTWILFSILFLLGLFMFKYINPRADALHALMNKREAFEL
                     LAHTMKAGSYFEVPQFNDIVSLLKAAPVALMEVIVRPNIFKISSPLVFLSALENMYIW
                     ILIVSALMFRKRNSVLIGQWNYFGLFVVIATFTLIGLTVPVEGAIVRYKSVVLPILMA
                     ILLCNIDYQKLFVRFPRIEKYFKL"
     gene            1253..2509
                     /locus_tag="PK736_07450"
     CDS             1253..2509
                     /locus_tag="PK736_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33263.1"
                     /translation="MQAFLFLLTAAAFFGLIFKQAFFKLNNISIKLLSILFGVKIIFI
                     FVHHYLFYKYGVNPDTYAMLRDAQSIWNFIFDDFKLALDIFFGIYDSHQQPIEVTNLE
                     VWNNYDTMYNDTRTLIRANVLLFVFTQGYYLVQCLWYSFFSFAGSIALYKTIAGVCAI
                     NDANKSKIIFAACCAWPSFLFWTSACFKETFLVAVAGFFIFSCYQYYQSRSFTKGIIA
                     LILLLLLFTIKIYYALLLIPVLLVFILLINARTIKMGQLLSIFVLGFVIFCFINYFVF
                     DVVTFMYNKRINFENMARDSHAVSLIKAPQFEPDIFSFLIHAPQALFNIICMPLKFST
                     PVFGLLSLENIFLLATIIFLILNFKWNKSYAMLSICILCFAILSLLIIGYTTPVLGAM
                     VRYKAPFIPFVLMFFALHFSSTKKAE"
     gene            complement(2877..3824)
                     /locus_tag="PK736_07455"
     CDS             complement(2877..3824)
                     /locus_tag="PK736_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRC33264.1"
                     /translation="MRYLKSLLVFALVLIFILPLCIQATGQSNENLTTTYAKADSIGI
                     FYAAQNFKTHQLATQVLISDCKTQLEKARAIFSFVAHRVKYDFNTNAYEADEVLKHKY
                     AVCQGYANLIDAMMTDAGIESKVITGYTKAQVNITHHDVTKTNHAWNAIKINNTWQLI
                     DATWGAGSYDYGTKKFTALFEENYFLADPEMMKYTHFPLDTSWQLLDKKVDAVTFINY
                     PFAWRGFEIHNITNISPDKFKLKYKAGEKFILSFTSDENVEHPLVNIEGERYSYPFEF
                     KNIKDKQYSSEIELKFKGTKQVTFFLNGTGVATYMVTVN"
BASE COUNT         1095 a          684 c          709 g         1482 t
ORIGIN      
        1 atagtggttt cttttattcg cggagggtac atgtactaaa ttttcagttg gcatttttac
       61 taaagcttgc ggctgcactc gtcatgactt ttatatatac ttatttttat cccgaccgcc
      121 atgtggccga tatattcagg tattttgatg atagcaatgt gcttattcaa atgtttgaaa
      181 acaacgtcag cgattttttt gcattcatca ccggtcacga taatgctgca ttgtacaaat
      241 ataaaattac aatgaataac tgggagtcgc ccggcaacgt ttacaataat aatcgcgtta
      301 tgattatcct taacgctgca atcggcttta tttcgttggg cgattattat gtgcacctaa
      361 ttctgtttac cttcttatct tttgttggcc ttactgctat gttcaaagtg tttaataaaa
      421 tttataaagt atatcagggc attgtattta tttctgtttt cactttgcca tctattattt
      481 tctggacatc gggagtgctg aaagaatcgc ttgtaatgtt tgctttagga atttttcttt
      541 acagctatat gcggttttat tttaatagcg ccatttggta tcatttattc tccttcgcat
      601 taggctttat tattttaatg ttgctaaagt tttatttgct catcatttta ttaccggcca
      661 tgataattct attgttaaca caacgaatat taattcttca aaaaatgaaa ggccttacat
      721 ggatactttt ttcaatacta tttttattgg gtttgtttat gttcaaatac ataaatcccc
      781 gtgccgatgc tttgcatgcg ttaatgaata aacgggaagc atttgagttg cttgcgcata
      841 caatgaaagc cggtagttat tttgaggttc cacagtttaa tgatattgta agtctgctaa
      901 aagctgcacc ggtggcactg atggaagtaa tcgtacggcc caatattttt aagataagtt
      961 cgccattggt ttttttgtcg gcattagaaa atatgtacat atggattttg atagtgtcgg
     1021 ccttaatgtt tcgcaagcgc aatagtgtgt tgattggaca gtggaattat tttggattgt
     1081 ttgtggtaat tgccacgttt acacttatag gtttaaccgt tcctgttgaa ggtgcaattg
     1141 tacgttacaa atcggtggtg cttcccatat tgatggcaat attattatgc aatatcgact
     1201 accaaaaatt gttcgtacgt tttcctcgca tcgaaaaata ttttaaactc tgatgcaggc
     1261 gtttttattt ctgcttacag ctgcggcctt ttttggcctg atattcaagc aggctttttt
     1321 taagttgaat aatatttcaa tcaaattact tagcatactg ttcggggtaa aaattatttt
     1381 cattttcgtt catcattatt tgttttataa atatggggtc aatcccgata catatgccat
     1441 gctgcgtgat gcgcaatcga tatggaattt tattttcgat gattttaaac tcgcccttga
     1501 tattttcttt ggcatttatg atagtcacca acaaccaata gaagtcacca atttagaagt
     1561 atggaataat tacgatacca tgtataacga tacccgaaca ttaattcgtg ctaatgtttt
     1621 gttgttcgtt tttactcagg gatattattt agtgcaatgc ctgtggtatt catttttttc
     1681 gtttgcggga agcatagctt tgtacaaaac tattgcaggc gtatgtgcca taaatgatgc
     1741 aaataaatca aagattattt ttgctgcatg ttgtgcatgg ccatcttttt tgttttggac
     1801 ttcagcatgt tttaaagaaa catttttggt tgccgttgca ggttttttta tttttagttg
     1861 ctaccaatat tatcagtcac gaagtttcac aaaaggtatt attgctttaa tccttttatt
     1921 gcttttgttt actatcaaga tttattatgc tctgctgttg attccggtgt tgttagtttt
     1981 tattttattg attaacgccc ggactattaa aatgggtcag ctgctttcta tttttgtatt
     2041 gggctttgtg atattttgtt tcataaatta ttttgtgttt gatgttgtta cgtttatgta
     2101 caacaagcga atcaatttcg aaaatatggc ccgcgattct catgctgttt ccttaataaa
     2161 agcaccacag tttgagcccg atattttttc ttttttaata catgcaccac aagcattgtt
     2221 caatataata tgtatgccac ttaaattttc cactccggtt tttggtttgt tatctttaga
     2281 aaatattttt ttgttggcca ctattatttt tttaatccta aattttaaat ggaataaaag
     2341 ctatgccatg ctatcaatat gtatactttg tttcgcaatt ttatctttgt tgataatagg
     2401 ttacacaaca ccagtattgg gcgctatggt aaggtacaaa gcaccattca ttccatttgt
     2461 gctgatgttt tttgctttac atttttcttc aaccaaaaag gcggaataaa aacaaatgta
     2521 ttttgcttcc ggtatttagc aactgctgta atcttttgcg aaactaattg gaattatttt
     2581 ttacctaccc gtattaggtg aggaaagagg atgaatgcta atttcagagt cctcatttat
     2641 tttttatttt tcaaaggcat gagagactct gaaattaatt aacgaagcaa agtacgaagc
     2701 agaggttgct tcgcagtttg agaatccctt cgactgcgct caggctgaca acactcactg
     2761 taacaattcg caatgacgaa ctagaaacta tgaatgccga caccttggtc ggcatcccga
     2821 ccgtagtgtc gggactaaaa actcaaaact tcaaactaga aactttaaac ttcaaactaa
     2881 ttaacagtca ccatatacgt agccacacct gttccgttca aaaaaaaagt tacttgtttt
     2941 gttcctttaa attttaattc tatttcgctg ctatattgct tgtctttgat gtttttaaat
     3001 tcgaaagggt aggagtaccg ttcaccttca atattgacaa gtgggtgttc tacgttttca
     3061 tcggaggtga acgaaagaat aaatttttca cctgccttat acttcaactt aaacttatcg
     3121 ggcgatatat tggtgatatt gtgtatttcg aaaccacgcc atgcaaaagg ataattgata
     3181 aacgttactg cgtccacttt cttatcaagt aattgccaac ttgtatcgag tggaaaatgt
     3241 gtgtacttca tcatttcggg atctgccaga aaataatttt cttcgaacaa tgcagtaaat
     3301 ttttttgtgc catagtcgta cgaacctgca ccccatgttg catctatcag ttgccaggtg
     3361 ttatttattt ttattgcatt ccatgcatga tttgttttcg ttacatcgtg gtgagttatg
     3421 ttcacctgtg ctttggtata gcctgttatt actttcgatt cgataccggc atcggtcatc
     3481 atagcatcta taaggtttgc atatccctgg cacacggcat atttatgttt cagcacttca
     3541 tcggcttcgt aagcatttgt attgaaatca tattttaccc ggtgcgcaac aaaactaaaa
     3601 atggcgcggg ctttttcaag ttgggtttta caatcgctta tcaatacttg tgttgccagt
     3661 tgatgcgttt tgaaattttg tgcagcataa aaaattccaa tgctatcagc ctttgcataa
     3721 gtagtagtca ggttttcatt tgattgaccc gtagcctgaa tacacaatgg caatatgaaa
     3781 attaacacca gggcaaaaac caacaagctt ttaagatacc tcatattttt gaaaatgata
     3841 ttgttgaaat tgaaacggca attgtattgt aaaattactg ctaatcaaat ttattttcgt
     3901 cagccttaaa taaagaaagc tgtgtaatac aaacgaggcc atttttttgc cacacaacta
     3961 ttaacaatgc
//
LOCUS       DAORQA010000163         3958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5542_length_3958_cov_10.056623, whole genome shotgun sequence.
ACCESSION   DAORQA010000163 DAORQA010000000
VERSION     DAORQA010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5542_length_3958_cov_10.056623"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>233
                     /locus_tag="PK736_07460"
     CDS             <1..>233
                     /locus_tag="PK736_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC33265.1"
                     /translation="SLLGITIGIFAIILVFTLVDGMERDVTTSIQSLGDNVIYVQKWP
                     WTFGENYPWWKYMSRPLPTYDEMESLKDRVENF"
     assembly_gap    234..243
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <244..1172
                     /locus_tag="PK736_07465"
     CDS             <244..1172
                     /locus_tag="PK736_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC33266.1"
                     /translation="IALGANTGGQLIKYKNKTIEDGSMFMVSHDYDKIRAFDLQQGRY
                     FTQIESDGGRNVCLIGYSIKQNLFENYEAVGKEISVKGRKLTVVGVFNKEGKSMLDNG
                     FDELILVPINYGRKVIDINSDRVDPFIMAKGVDGFTNEQLKEELRGAMRSIRRLRPVQ
                     EDDFALNESKLIANNITGIFDILQIVGWIIGDLSILVGGFGIANILFVSVKERTNLIG
                     IQKSLGAKNFFILLQFLFEAVLLCLIGGIIGIFLVYLLSLALEGAVPFPLSLTVSNIV
                     LGLTVSANIGIIAGFLPAYTASQLDPVEAIRS"
     gene            1192..1536
                     /locus_tag="PK736_07470"
     CDS             1192..1536
                     /locus_tag="PK736_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33267.1"
                     /translation="MNKALITLPKPLKIFAIIGLIMVIISIPSIFSPFIKKLGTFYPL
                     LFGSMVSLRFMALIGVWYMKKWGPILFLIVFLAYEITSMMVGISRDIELPLSTILCLV
                     FLTYVPRMDDNL"
     gene            1646..2908
                     /locus_tag="PK736_07475"
     CDS             1646..2908
                     /locus_tag="PK736_07475"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRC33268.1"
                     /translation="MQVFKFGGASVKDASSIKNIKSILSRYTADSLVVVVSAMGKTTN
                     ALEKVVNAHFNNDDNATQLLNEVKLFHDEIIANLFPVNHPVIIEVNNFFVEAEWMLEE
                     KPRSYNFLYDQIVSIGELVSTKIISQYLLLQQMDNTWIDARGIILTDNNYRNANIDWL
                     QTETIIVETVTSQLKKKNIVITQGFIGGTSENYTTTLGREGSDYTAAIIAYCMKAHSV
                     TIWKDVPGVLNADPRFFDDTQKLEQISYLDAIEMTYYGATVIHPKTIKPLENRGIPLY
                     VKSFLQPEGAGTIIGKDLNTKPFVPNLIVKTNQVLISISATDFSFIAEDNLSAIFNIF
                     ATQGIKINLMQNSAISFSVCLDNDAIALPTLLDALKQHFKVRYNDNLTLYTIRHYNQF
                     TLDGLLKGKTLILEQRSRNTAQIITRPN"
     gene            2914..3528
                     /locus_tag="PK736_07480"
     CDS             2914..3528
                     /locus_tag="PK736_07480"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HRC33269.1"
                     /translation="MYIPKHYKEEDMVHLVAFMNQYPFALLISQVDGKPFATHLPFII
                     EKNQNGILLQSHMALANPQWKQLAGQQVLIVFSEPHAYISPSLYEKKENVPTWNYIAV
                     HAYGHTTLIENKDKVEQLLHKTISTFEPAYVAQYKDLNSDYLRRMVKGIKAFSIEVND
                     VQGKFKLSQNKTIVEQKNIVDHLQKQNENTSVAIAQYMKNKLPE"
     gene            3574..>3958
                     /locus_tag="PK736_07485"
     CDS             3574..>3958
                     /locus_tag="PK736_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRC33270.1"
                     /translation="MKKRNRPIIIWLYSGCALVFLMVIIGGITRLTGSGLSITEWKVV
                     TGTLPPMNEAQWASEFSKYQASPQFQKVNSHFQLHDFKTIYWWEYIHRLVARLMGVVF
                     VIPLLWFLFKRKIQADLIPKLFIIIL"
BASE COUNT         1258 a          717 c          752 g         1221 t
ORIGIN      
        1 tcattactcg gaattactat aggcatattc gccatcattc ttgtttttac tttggtcgat
       61 ggtatggagc gcgatgttac taccagtata caatcgcttg gcgataatgt gatttatgta
      121 caaaaatggc cgtggacatt tggcgaaaat tacccttggt ggaaatacat gtcgcgccca
      181 ttgccaacct acgatgaaat ggaaagctta aaagaccgtg tcgaaaattt taannnnnnn
      241 nnnccatagc cctgggtgcc aacaccggtg ggcaattgat aaaatataag aacaaaacga
      301 ttgaagatgg cagcatgttt atggtatcgc acgattatga taaaattaga gctttcgatt
      361 tacaacaggg ccgttatttt acacaaatag aaagtgacgg tggccgtaat gtatgcctca
      421 ttgggtacag cataaaacaa aatctgttcg aaaattatga agctgtgggc aaagaaattt
      481 ctgtaaaagg ccgcaaactt acagtggtgg gtgtgtttaa taaagaagga aaaagtatgc
      541 tcgataatgg tttcgatgaa ttgatattgg tgccaataaa ttatggccgc aaagtaatag
      601 acattaacag cgatagagtc gatccattta taatggccaa aggcgtggat ggatttacaa
      661 acgaacaatt gaaagaagaa ttacgtggtg ccatgcgttc cattcgcaga ttgcgccctg
      721 tgcaagagga tgatttcgca ttgaacgaaa gcaaattaat agcaaataac attacaggta
      781 tattcgatat tttgcaaatt gtaggctgga ttattggcga tctttctatt ttggttggcg
      841 gttttggcat tgccaatatt ttatttgtat cggttaaaga gcgaaccaat ttaattggca
      901 ttcaaaaatc tttaggggca aaaaatttct ttatccttct tcaatttctg ttcgaagctg
      961 ttttgctttg cttaattgga ggtatcatag ggatttttct tgtttacctt ctatcacttg
     1021 cactcgaagg cgcagtacca ttcccattat cacttactgt ttccaacata gttttaggat
     1081 taacggtatc ggcaaatatt ggcatcattg cggggttttt gccggcatac accgcatcgc
     1141 aactcgaccc tgtagaagcc atccgcagtt aatttaattt gtaatcgcaa catgaataaa
     1201 gcactcatca cattacccaa gccactaaag atatttgcaa tcattggatt gataatggta
     1261 ataatttcta tcccatctat attttcacca ttcataaaaa agctcggtac gttttaccca
     1321 ttactttttg gctctatggt gtcgcttcgt tttatggcgc taataggagt ttggtatatg
     1381 aaaaaatggg gacccatttt atttcttatc gtttttttgg catatgaaat tacgagtatg
     1441 atggtgggca tttcgcgcga catagagttg ccgctctcaa ctattctgtg ccttgttttt
     1501 ttaacttatg tgccacgcat ggacgataat ttgtaattgc attttcattt cggttctaaa
     1561 atcataattt catttttttc gtgtcgatgt tttttttctt attgataaaa tagccgttac
     1621 attcgcgcaa cttttaattt ccaatatgca ggtttttaag tttggtggcg cttcggtaaa
     1681 agatgcttct tcaataaaaa atataaaatc tattctatcc cgttatactg ccgactccct
     1741 tgtggtagtg gtttcggcta tgggcaaaac caccaatgcg ctcgaaaaag tagtgaatgc
     1801 tcatttcaac aacgatgata atgccacaca attgttgaat gaggtaaagc tatttcatga
     1861 tgaaattatt gccaatctgt tccctgtcaa tcatcctgtg attattgaag taaacaattt
     1921 ttttgttgaa gccgaatgga tgctggaaga aaaaccccgc agttataatt ttttgtacga
     1981 tcaaatagta tccataggcg agttggtttc tacaaaaata attagtcaat atttattgtt
     2041 gcaacaaatg gataatacat ggatagatgc gcggggtata attcttaccg ataataatta
     2101 ccgcaatgca aatatcgatt ggctgcaaac cgaaacaata attgttgaaa cagtaacttc
     2161 tcaattaaag aaaaaaaata ttgtgattac tcagggcttc attggtggca cttccgaaaa
     2221 ttatacaact acactgggcc gcgaaggttc cgattacaca gcagctatta ttgcatattg
     2281 catgaaagcc cattcggtta caatatggaa agatgtgccg ggtgtactaa atgccgaccc
     2341 gcgctttttt gacgatactc aaaaactgga acaaataagt tatctcgatg caatagaaat
     2401 gacatattat ggcgctacag ttattcatcc taaaacaata aagcccctcg agaatcgtgg
     2461 cattccgctg tatgtaaaat cctttttgca acccgaaggt gccggcacta tcatcggtaa
     2521 agatttaaat acaaaacctt ttgtgcctaa tttgattgtg aaaactaatc aagtgctgat
     2581 aagtatttct gccaccgatt tctctttcat agccgaagat aacctgagtg caatatttaa
     2641 tatttttgca acacaaggaa taaaaatcaa cctgatgcaa aattccgcaa tcagtttttc
     2701 ggtatgctta gataacgatg caattgcttt acctacatta ctcgatgcat tgaaacagca
     2761 tttcaaagtg cgctacaacg ataatcttac attgtatacc atacgccatt acaatcagtt
     2821 tacactcgat ggtttattga aaggtaaaac actaatactt gaacaacgca gccgcaatac
     2881 agcacaaata attacacgcc caaattaatc accatgtaca ttccaaaaca ttataaagaa
     2941 gaagacatgg tgcatcttgt tgcttttatg aatcaatatc catttgcgtt gctcataagc
     3001 caagtagatg gcaaaccatt tgccacacat ttgcctttca ttatcgaaaa aaaccaaaac
     3061 ggcattttgt tgcaaagtca catggcattg gcaaacccgc agtggaagca actcgcaggg
     3121 cagcaggtgc tcattgtttt tagcgagcct catgcgtata tttcaccatc gctatatgaa
     3181 aaaaaagaaa atgtccccac ctggaattac attgccgtgc acgcatatgg ccatactacg
     3241 ttgatagaaa ataaggataa agttgaacaa ttgttacaca aaacaatttc tacatttgag
     3301 cccgcatatg ttgcacaata caaagatttg aattctgatt acttgaggcg aatggtaaaa
     3361 ggaataaaag cattttctat tgaagtgaat gatgtgcaag gcaagttcaa attgagtcaa
     3421 aataaaacaa ttgttgaaca aaaaaatatt gttgatcatt tgcagaaaca aaatgaaaac
     3481 acaagtgttg caatagcaca atacatgaaa aataaattgc cggagtaatt taattgtctt
     3541 gcaataacga aatagaagat tgatataacg taaatgaaaa aaagaaacag gcccatcata
     3601 atttggttgt acagtggatg tgcacttgtt tttcttatgg tgattattgg tggtataaca
     3661 aggcttaccg gtagcggcct ttccattacg gaatggaagg tagtaacggg aacactacct
     3721 ccaatgaacg aagcacaatg ggcctcggag ttttcaaaat atcaagcctc accacagttt
     3781 caaaaagtaa attctcattt tcaattacac gattttaaaa ccatttattg gtgggagtac
     3841 attcaccgtc ttgttgcgcg attgatgggt gttgttttcg ttattccttt gctttggttt
     3901 ttgtttaaaa gaaaaattca ggccgattta attcccaaac tgtttatcat tattttgc
//
LOCUS       DAORQA010000164         3929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5594_length_3929_cov_7.286784, whole genome shotgun sequence.
ACCESSION   DAORQA010000164 DAORQA010000000
VERSION     DAORQA010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5594_length_3929_cov_7.286784"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>966
                     /locus_tag="PK736_07490"
     CDS             <1..>966
                     /locus_tag="PK736_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33271.1"
                     /translation="IIVPPPKTLAAMEERIAETCGDTLTYVIPSIVTNTYTFNSVSFQ
                     WTTTGTGTFTNQLFGAALYNASNADIANGSTKLYFQLIDNANECSYLKDSMIVWYNPH
                     YTVDAGADTLVLCADKNAVLTGSASANVISTTWTTSGSGTFANDTMLATTYFFSPNDT
                     ANRKVTLYLTTDNPVGSCAAVIDSIIIIIDPLPNASAGTNSPVCYGNDLNITGTGGTT
                     YSWSGPNAYSSTLQNIVRSPADLTMNGTYTVTVTDTKGCSATATTVADVQVCGCIIPQ
                     VAVLKNNVNCFGGNNAFVILTISTPGNHTFNWSNGSTAQNQTNLV"
     assembly_gap    967..976
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <977..2784
                     /locus_tag="PK736_07495"
     CDS             <977..2784
                     /locus_tag="PK736_07495"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33272.1"
                     /translation="TVTVTNIPGCDTTITIQIIEPLAPLVATCNGTDISCNGSNTGVV
                     SVSVTGGTGPYTYVWNNGVTISSASGLNAGTYTVIVTDAKGCTSSCSRIIAQPAMPLS
                     VNCSGTNVTCLSANNGTAQAIAAGGTSPYTYLWSNGFTGASISSLAAGTYSLTATDTN
                     GCTATCSYTITQSGALNVTLNTTNVSCGVANSGQVEAIVSGGSSPYNYLWSNGSTTSV
                     NIGLAAGTYTVNVTDANGCTKTGSATVSNSGGAPAQPGVISGVSPVCRNSIGNIYSIV
                     AVPGATSYLWTTGAGATIMSGQGGTSISVSFSNTANANSITVAAVNLCGSSAVRVFKY
                     SIGSVPLTPGIISGQTYGLCNGTNKNYNINPVTYATSYFWTVPAGANIVSGQGTTQIA
                     VNFTNSFTTSGKIKVRAINACGQSADQQLNIVAKPSQPKILGSNYACQYQQGVTYSIT
                     PVIGATSYVWTIVSGSTLVSGQGTSTIVVNWGSINGVISCRAINACGQSVLAKHPVSF
                     TCTKEGLPFDISDLQLLPNPTNGLTIQFTSFTFDNEAYFEIYNIIGAKVYTENISATE
                     GENKYSFDANRFAPGCYTVKLKIKGSTEMLKMIVN"
     assembly_gap    2942..2964
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2978..3580)
                     /locus_tag="PK736_07500"
     CDS             complement(2978..3580)
                     /locus_tag="PK736_07500"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRC33273.1"
                     /translation="MAFTLPSLPYEFAALEPHIDARTMEIHHGKHHNAYVTNLNNAIA
                     NTPADGMSIEEICKNISQYSMPVRNNGGGHYNHSMFWQIMSPKGGGVPTGTLGDAITK
                     TFGSFDAMKEKFAAAATTRFGSGWAWLIKNDAGELAICSTPNQDNPLMDIAEVRGTPL
                     LGIDVWEHAYYLHYQNRRPDYIAAFWNVINWEEVARRGGM"
BASE COUNT         1154 a          794 c          789 g         1159 t
ORIGIN      
        1 tgattattgt gccaccacca aaaactttag cagcaatgga agagcgaata gcagaaacct
       61 gtggtgatac cttaacatat gttataccgt ccattgttac taatacatat actttcaatt
      121 cagtttcttt tcaatggaca actaccggaa ccggaacttt taccaatcaa ctatttggtg
      181 ctgcactcta taatgcttcc aatgctgata ttgcaaacgg tagcacaaaa ttgtatttcc
      241 agttgataga taatgcaaac gaatgcagtt atttaaagga cagtatgata gtatggtaca
      301 atcctcatta cacggtggat gcaggcgcag ataccttagt attatgtgca gataagaatg
      361 cagtgttgac aggtagtgcc agcgcaaatg tgatttcaac aacatggaca acaagtggta
      421 gcggaacttt tgcaaacgat actatgttag ccactactta cttcttttcg ccaaatgata
      481 ctgctaacag aaaagtaacc ttgtacctca ctactgataa tcctgtgggt tcgtgtgctg
      541 ctgtaattga tagcatcata ataattatag atcctttgcc aaacgcatct gccggtacca
      601 atagccccgt atgttatggc aacgatttga atattaccgg aacaggtggc accacttatt
      661 catggtcggg tccaaatgcc tattcatcta ctttgcaaaa tatagtccgc tctcctgccg
      721 acttaacaat gaacggtacg tatactgtta cagttaccga cactaaagga tgctcggcaa
      781 ccgccactac agttgccgat gtacaagtat gtggttgtat aatacctcag gttgcagtgc
      841 tgaaaaacaa tgtgaattgt tttggtggca acaatgcctt tgttattctt accattagca
      901 ccccgggcaa ccatacgttt aattggagca atggctctac ggcacaaaac caaacgaacc
      961 tcgtggnnnn nnnnnnatac cgtaacggtc acaaatattc cgggttgcga taccacaatt
     1021 actattcaaa taattgagcc acttgctccg cttgttgcca cttgcaacgg tacagacatt
     1081 tcttgtaatg gatccaatac cggtgtagtt tctgttagcg taacaggtgg taccggtcct
     1141 tacacttacg tttggaacaa tggagttact atttcatcgg caagtggatt gaatgcaggt
     1201 acgtatactg ttatagttac ggacgcaaaa ggatgcacaa gcagttgctc acgaattatc
     1261 gctcaaccgg caatgccttt gtcagttaat tgtagtggta caaatgtaac atgcttaagc
     1321 gccaataatg gaacagcaca ggctattgct gccggtggta cctcacccta tacttatttg
     1381 tggagcaatg gatttaccgg tgccagtatc tcatcgcttg ctgcagggac atattcactt
     1441 actgcaaccg ataccaatgg ttgtactgca acatgcagtt acacaattac acaatcgggt
     1501 gcacttaacg taactttaaa cacaaccaat gtttcctgtg gggttgctaa tagtgggcag
     1561 gttgaggcca ttgtaagcgg aggcagttca ccgtacaatt atttatggag caatggatca
     1621 acaacatcag taaatattgg gttggccgca ggaacgtata cagttaatgt tactgatgcc
     1681 aatggatgta caaaaaccgg ttctgccaca gtttcgaatt cgggcggtgc ccctgcgcag
     1741 ccgggcgtaa tttccggagt ttcgccagtt tgccgaaaca gcattggtaa catatattct
     1801 atagtagctg tgcccggagc cacgagttat ttgtggacta caggcgcagg tgctaccatt
     1861 atgagcgggc agggaggcac ttcaataagc gttagttttt ctaataccgc aaatgcaaat
     1921 tcaattacgg tagctgcagt aaacttatgt ggttcaagtg cggtgcgtgt ttttaaatat
     1981 tctattggtt cggttccgct tactcccggc attattagcg ggcaaacgta tggattgtgt
     2041 aacggcacaa ataaaaatta caatataaat cctgttacct atgccacatc atatttttgg
     2101 acagtgcccg caggtgccaa tattgtaagt ggacaaggca ccacacagat tgccgtaaac
     2161 tttacaaata gctttaccac ttcgggaaaa ataaaagtga gagcaattaa tgcatgcggc
     2221 caaagtgccg atcagcagtt aaatattgtt gcaaagccat cacagccaaa aatattggga
     2281 tcgaattacg catgtcaata ccaacaggga gttacttatt ctattactcc tgtgattggt
     2341 gctacatctt atgtttggac tattgtttcg ggtagcacgc tggtaagtgg acaaggcacc
     2401 tccactattg ttgtaaattg gggaagcatt aacggtgtaa tatcgtgccg ggcaataaac
     2461 gcttgcggcc aaagtgtatt ggccaagcat ccggtttctt ttacctgcac caaagaagga
     2521 ttgcctttcg atatttccga tttgcaattg ttacccaatc caacaaacgg attaacaatt
     2581 cagttcacat catttacatt tgataatgaa gcgtattttg aaatctacaa tatcattggc
     2641 gcaaaagttt acactgaaaa tatatcggca accgaaggag aaaacaaata ttctttcgat
     2701 gctaatcgtt ttgctccggg ttgttatacg gtgaagctga aaataaaggg ctcaacagaa
     2761 atgttgaaaa tgattgtgaa ttgatggtta tgttttttta aacctttttt gttttaaaaa
     2821 aatttaatgt aaaggtaaaa ttcaagcccg gtcatacata caaagcattc ataaatttta
     2881 atcgtttatt aaacaaacag aaaaaaaagt tccaagcata aaaaaaggtt gcccgttatt
     2941 gnnnnnnnnn nnnnnnnnnn nnnnagaata tattgaatta cattcctcct ctgcgggcca
     3001 cttcttccca gtttattaca ttccaaaatg cagcaatgta atcgggtctg cggttttgat
     3061 agtgtaaata atatgcatgt tcccatacat caatgcctaa caatggagtg ccgcgcactt
     3121 cggcaatatc catcaatgga ttgtcttgat tgggtgtgct gcaaatagcc agttcaccgg
     3181 catcattttt tatgagccat gcccatccgc taccaaaacg tgtagtagca gcagcggcaa
     3241 atttttcttt catagcatcg aaactgccaa atgtttttgt aatggcatcg cccaatgttc
     3301 cagttggtac acctccgcct tttggcgaca ttatttgcca aaacatactg tggttgtaat
     3361 gcccgccacc attattccga acaggcatac tgtattgcga aatattttta catatttctt
     3421 caatgctcat accatcagca ggggtgttgg caattgcatt gttcaggttg gtaacataag
     3481 cattgtggtg tttaccatga tgtatttcca tggtgcgcgc atcgatgtgc ggctcgagtg
     3541 ctgcaaactc ataaggcagc gaaggaagtg tgaaagccat atttatattt tttaaaattt
     3601 atcttcttgc aaaagtaatc aatgcaacgg tagtgtaaca ataaataatt attctttgtt
     3661 taccatatgg tcaaatatgc cgatactgct tttgttttca aaaatgaaaa acaaaagtgt
     3721 gcggcattac gaaaagtacg tttagttttt tttaaaacca aactactttt cgtaaacaat
     3781 acaattcata ctctcggtca ggcgaaccat cattccttcg acaaatgcat ttgatttata
     3841 ttttgtaaga aattgtttta attgttcctt atcctgaaat gtattcgcat tttctaatgt
     3901 gcccatacca taaaatttgc cttcatcaa
//
LOCUS       DAORQA010000165         3924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5602_length_3924_cov_5.853709, whole genome shotgun sequence.
ACCESSION   DAORQA010000165 DAORQA010000000
VERSION     DAORQA010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5602_length_3924_cov_5.853709"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1764
                     /locus_tag="PK736_07505"
     CDS             229..1764
                     /locus_tag="PK736_07505"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33274.1"
                     /translation="MISLFGKIYSVFLFLICINSICIAQPQKQNKVNPNGYNRFFYAN
                     GNLSSEGNMVNGKPEGYWRTYFENGNVKSEGNRKNNMIDSLWTFYFPDKKISAQYNYL
                     KGVKNGMQKDFYPNGNIRSEETLLQNIKQGTCNSYFENGRIKSKAKFDKGRQDGVTIN
                     YNSQGDIISLQTFKNGYLTADEKINRRDKFGLKQGAWKAFYDNMQIKSEGVWDDDKKN
                     GVFKEYTADGQILKTEVYKNDELLVQQLTEAVKIEVQKQYHANGKVKNSVTIVEGVKQ
                     GYSNEYDTAGNILATTVYSDNKILSQGLIDNSGKAQGSWTHFYDDGSAKAKGSYTDGK
                     KTGDWQYFYKGNITQQSGKYVNGKPHGQWIWKYDNGQILRSENYVNGKEEGQSTEYNE
                     FGKMIAQGNYTSGAKEGLWTFNNGEYIATGMYVDNEQDGIWKTYNADSTLYSEGKYLQ
                     GLQQGNFKLYHHNGQIRELQHYKDGIRIGDWKFFDEQGVLLYTITYAGDVIQKIDGVR
                     MRQ"
     gene            1827..2204
                     /locus_tag="PK736_07510"
     CDS             1827..2204
                     /locus_tag="PK736_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HRC33275.1"
                     /translation="MALELEGKVYKIGTEVTGEGKNGPWKKIDFMIEYLDGQYPKKAA
                     FSAWTDKVEMVKKLNIGDTVTVSFNIDSREYNDKFFTDLRIWKMQIGGTAGASAASST
                     QSAPQAVSAEPMSAPTADDDLPF"
     gene            2310..>2636
                     /locus_tag="PK736_07515"
     CDS             2310..>2636
                     /locus_tag="PK736_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRC33276.1"
                     /translation="MSFYTLSKIQRAQLVIEIYNDILADIRYNTTEKVLLYFNDDDTY
                     IRKAAYLNIGKIYLNHKEFAITTIALLHKLLLSEEYKIRQTVINAAGEIGKKDFAIVE
                     IFFDTAL"
     assembly_gap    2637..2646
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2647..3080
                     /locus_tag="PK736_07520"
     CDS             <2647..3080
                     /locus_tag="PK736_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRC33277.1"
                     /translation="DKHHSVRNAVIGSIKKMSEKNPLPVLQWAQKYLHHPDKEIRREI
                     CHGIELRGRTHPQDILPMLKQLQYDTTARVRNTLVHVLGQIAYKKGCLQTVIEHLNNW
                     ENKILVKQAIEEIIDVHYRYRNFATLTQEQAEEYIKHNYKN"
     gene            3084..3311
                     /locus_tag="PK736_07525"
     CDS             3084..3311
                     /locus_tag="PK736_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HRC33278.1"
                     /translation="MEVPQANPQLLIELVTMKMPFGKYKDRILCDLPVSYLEWFQRKG
                     FPAGKLGVLLTTLYEIKLNGLEDLLKPLKNK"
     gene            3334..3804
                     /locus_tag="PK736_07530"
     CDS             3334..3804
                     /locus_tag="PK736_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33279.1"
                     /translation="MKASTIAELKKELQLLPQKQLVAHCIRMAQFKVMNKELLSYLLF
                     EQNDEQQFVDNVMEEMDAMFKEINSSNSFFLLKKSIRKILRHTHKHIKFSQIKTTEIQ
                     LLMHFCQSLKNLNIGFEENKVLLNMYNAQVKKIHKAIETLHPDERLDYNADLDL"
BASE COUNT         1433 a          599 c          768 g         1114 t
ORIGIN      
        1 ttgttcttcg ttcttcgttt cgtttgttgt gaggattgtt cttcgttctt cgttttatat
       61 gcgcagcggg ttgtttttag ttttatattc atttcagagt ctctcatgcc tttgaaaaat
      121 aaatgataaa cgaggactct gaaatgtata tgttttgttc ttagttacga tttcgttgat
      181 gattattttt gacttattcc ttttgattta ttccttttaa cttatttaat gatttctctt
      241 tttggtaaaa tatattctgt ttttttattt ttgatttgta tcaattcaat ttgtattgcg
      301 caaccgcaaa agcaaaacaa agtaaaccca aatggttaca acaggttttt ttatgccaat
      361 ggaaatttga gcagcgaagg caatatggtg aatggtaaac ccgaaggtta ctggcgcaca
      421 tatttcgaaa atggaaatgt aaaatcggag ggcaaccgca aaaataatat gatcgatagt
      481 ttgtggactt tttattttcc cgacaaaaaa atatcggcac aatacaatta tttgaaaggg
      541 gtaaaaaatg gaatgcagaa agatttttat cccaatggaa atattcgcag tgaagaaact
      601 ttgttgcaaa atattaaaca aggaacatgc aattcttatt tcgaaaatgg tcgcataaaa
      661 tcgaaagcta aatttgacaa aggcaggcaa gatggtgtta ccatcaatta taattcgcaa
      721 ggtgatatta ttagtttgca aacttttaaa aatggatact taacagcgga cgaaaaaata
      781 aacaggcgcg ataagtttgg attgaagcaa ggcgcatgga aagcgtttta cgataacatg
      841 caaattaaaa gcgaaggagt gtgggacgat gataaaaaaa atggcgtgtt caaagagtat
      901 actgccgatg ggcaaatact aaaaacagaa gtttataaaa acgatgaatt gcttgtacaa
      961 caacttaccg aagctgtaaa aattgaagtg caaaaacaat atcatgcaaa tggcaaagta
     1021 aaaaacagtg ttacaatagt tgaaggggta aaacaagggt atagcaacga gtacgacacc
     1081 gcgggaaata ttttggcaac tacagtttat agcgataata aaatactgtc gcaaggcttg
     1141 attgataaca gtggtaaagc acaagggtca tggacacatt tttatgatga cggcagtgcc
     1201 aaagcaaaag gctcgtatac cgatggaaaa aaaactggcg attggcaata tttttacaaa
     1261 ggcaacatca cacaacaaag tggaaaatat gttaacggaa aaccccatgg acaatggata
     1321 tggaaatatg ataatggcca aatactacgc agcgaaaatt atgtgaatgg aaaggaagaa
     1381 ggacaaagta ccgagtacaa tgagtttggt aaaatgatag ctcagggcaa ttacacctca
     1441 ggggcaaaag aaggtttgtg gacttttaat aatggagagt atattgccac aggaatgtat
     1501 gttgacaatg agcaagatgg tatttggaaa acgtataatg ctgatagcac attgtacagc
     1561 gaaggaaaat atttgcaggg gctacaacag ggtaatttta aattgtatca ccacaatggg
     1621 caaatacgtg aattgcaaca ttacaaagat ggaataagaa taggcgactg gaagtttttt
     1681 gacgagcagg gtgtattgct ttacacaatt acttatgcag gcgatgtgat acaaaagata
     1741 gatggcgtgc gtatgcgaca ataattttca acaatagctt ttggttcgtt aaatatttat
     1801 tatttttaca aaaaaaaatc aacattatgg cattagaatt agaaggaaaa gtttacaaaa
     1861 ttggaaccga agtaacagga gaaggaaaga acggaccgtg gaaaaaaata gatttcatga
     1921 ttgaatatct tgatggacag tatccaaaaa aagcagcatt ttctgcatgg accgacaaag
     1981 tagagatggt taaaaaattg aatattggcg atactgttac agtttcgttt aatattgaca
     2041 gtcgtgagta taacgacaaa ttttttaccg acctgcgcat atggaaaatg caaataggtg
     2101 gaactgccgg agccagtgct gcaagcagta ctcaaagtgc accacaagca gttagtgcag
     2161 agcctatgtc ggcaccaacg gccgatgacg atctgccgtt ttaataaggc ctctttattt
     2221 tatatatagc tttaccggca attgatttat tatatcgatt gccggtaatt ttttttacct
     2281 gcttcaagct aaagcaataa attttttaaa tgagttttta tactttatca aaaatccaaa
     2341 gggcacaatt agttatcgaa atttacaacg atattcttgc agatataaga tacaacacaa
     2401 ccgaaaaagt attgctttat tttaatgatg acgatactta tatacgaaaa gctgcctatt
     2461 taaatattgg taaaatttat ttgaaccata aagagtttgc cattacaaca atagcactgt
     2521 tgcataaatt attactgagc gaagaatata aaataaggca aacggtaata aatgcggctg
     2581 gcgaaattgg aaagaaagat ttcgcgattg tcgaaatatt ttttgacaca gcattgnnnn
     2641 nnnnnntcga taagcatcat tcagttcgaa atgctgttat tggttctata aaaaagatga
     2701 gcgaaaaaaa tcctctgcct gttttacaat gggcacaaaa atatttgcat caccccgata
     2761 aagaaatacg cagagaaatt tgtcacggta tagaattacg agggcgtaca catccccagg
     2821 atattttacc tatgttgaaa caattgcaat atgataccac tgcccgggta cgaaatacac
     2881 ttgtgcatgt gcttggacag atagcgtata aaaaaggttg cctgcaaaca gtgattgaac
     2941 atttaaataa ttgggaaaat aaaatactgg taaagcaagc catcgaagaa ataattgatg
     3001 tgcattatcg ctatcgtaac tttgcaacgc ttacacaaga gcaagccgaa gaatatatta
     3061 agcataatta taaaaattaa ataatggaag taccacaagc caatccacag ttgttaattg
     3121 aattggtaac aatgaaaatg ccttttggca aatataagga ccgcatactt tgcgaccttc
     3181 ctgtttcata cttagaatgg tttcaacgca aaggatttcc tgcgggtaag ttaggtgtgc
     3241 tgttaaccac actgtacgaa ataaaattga acggcctcga agatttattg aagccattaa
     3301 aaaataaata gcccgcattt attttataaa agaatgaaag cctccaccat agcagaatta
     3361 aaaaaagaac tacaactgtt accacaaaaa cagcttgtag cccactgtat tcgtatggct
     3421 caatttaagg taatgaataa ggaattgttg agctatcttt tatttgagca aaacgatgag
     3481 cagcaatttg tagataatgt aatggaagaa atggatgcca tgttcaaaga aataaattcc
     3541 agtaatagtt tttttctctt gaaaaaatcg atacgcaaaa tattacgaca cacccataag
     3601 catattaaat tttcgcaaat aaaaactaca gagatacaat tgctcatgca tttttgccag
     3661 agcctaaaaa atttgaatat aggcttcgaa gaaaataaag tgctattgaa tatgtacaat
     3721 gcacaagtaa aaaaaataca caaggccatc gaaaccttgc accccgatga acggttagat
     3781 tataatgcgg atttggattt gtagttgggc ataatcaact attatttatg actttgcctg
     3841 aaacgcacga gttatatcaa aaccccgtaa aatttgtaaa tcctggcgta tttaaaatca
     3901 acataacagc ggcaaagcat agat
//
LOCUS       DAORQA010000166         3923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5604_length_3923_cov_9.057394, whole genome shotgun sequence.
ACCESSION   DAORQA010000166 DAORQA010000000
VERSION     DAORQA010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5604_length_3923_cov_9.057394"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PK736_07535"
     CDS             complement(<1..221)
                     /locus_tag="PK736_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium symporter"
                     /protein_id="HRC33280.1"
                     /translation="MTQNKEAWGSRIGLILAMAGNAVGLGNFLRFPVQAVQNGGGSFI
                     IPYLICFLLMGIPLLYVEWAMGRFGGKHG"
     gene            complement(522..998)
                     /locus_tag="PK736_07540"
     CDS             complement(522..998)
                     /locus_tag="PK736_07540"
                     /inference="COORDINATES: protein motif:HMM:NF028004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HRC33281.1"
                     /translation="MKAKKIIHAVFMISVFALIVSSCKKDKAEEDANQALIDEVNSSG
                     YTYYQNGTLLSGVSPSPHGSFKLRFNNIAKAALVGNSFPTGSIIVKELFVGTTLDQFA
                     VMKKDASNANAGSGWVWAEFKPDGTVTYSAANKGSGCIGCHSGTPNRDLIRTFDLH"
     gene            1189..2802
                     /gene="lnt"
                     /locus_tag="PK736_07545"
     CDS             1189..2802
                     /gene="lnt"
                     /locus_tag="PK736_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRC33282.1"
                     /translation="MTQQKSGFVSSMRINNSVLSTFTGVLLWAAWPANGVAPLLWVAF
                     IPLLIIEWRFYNHSDQNGKSPFRYFYFTFVLFNLFTTWWVWNASAFGVIMAIVLNSLF
                     MATVFWLFYKCHKILKSDLAYFLLPVFWLSFEYMHMDWDLSWPWLTLANGWAAWYKCV
                     QWYEFTGFSGGTIWTWAANIFLFLAIKHHAQPLAKKYFRLALSVIIFPLVLSYTIYFT
                     YTEKINPVNIVVAQPNIDPYNEKFVGDNFSHIEMMLKLASTKIDTTTDYYVTPETAIA
                     SGIWEEQLYNHRTIRLMQNLVKQYPQLSIVTGAATDKFYRKGENKSVTARKFSETDDY
                     YDSYNTALQIDATDSIQIYHKSKLVPGVEKMPFEFIFKHMEQFAIDLGGASGSLGNQN
                     DVSVFKTQKHTIAPVICYESIYGKYVGKYVRKGADLIFIITNDGWWGNTPGYRQHLQY
                     ARLRAIEMRRSIARSANTGISCFINQKGDIIQQAQWWQTDVLKQTINANTTMTVYARI
                     GDIIAIVSCLLSLIALLWCGAITFRNKAN"
     gene            complement(2838..>3923)
                     /locus_tag="PK736_07550"
     CDS             complement(2838..>3923)
                     /locus_tag="PK736_07550"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRC33283.1"
                     /translation="MVVLAIFISSYFAFLSKYYLKKAGWTAAAIWLIIFLFILESLTG
                     LFIMCVILFVLAGIQLVRSKKYIPLSLMFVLAVCTLAWSSFYFINQMNALGNCNENES
                     PPTEKYTPNGNLYQHVQSYKGCENGHRLGWYICWNELRKSWNERSKFSFDSLDNRKQP
                     LPYTLIRYLTSKNLHKDSAGVYALSNDDLKAVEQGIANCTHVNMNGLQLRFVEIAYEL
                     NQYKKTGNASGFSIAQRIEYNKGSWYILKNNLLFGVGTGDNKIAFENYYNTTNSKLGQ
                     KWRLRSHNQYMAIAVSFGMVGLALFIFALLYPVIKLNAQRHFIFLVFFSISLLSFFNE
                     DTLETQAGITFFIFFYAVILWGYLRKV"
BASE COUNT         1237 a          744 c          728 g         1214 t
ORIGIN      
        1 tggccatgct tacccccaaa ccgacccata gcccactcga catacaggag tggaataccc
       61 atcaataaaa agcaaattaa atacggaatg ataaatgagc caccgccatt ttgaactgcc
      121 tgtaccggaa atcgaagaaa gtttccaaga cccacagcat tgccggccat tgctaaaatg
      181 agacctatgc gcgaacccca cgcttctttg ttttgtgtca tacaaataaa ttttgtttag
      241 taaaaattgt gatgaagata tcgatggcga aatatagaaa taatattcga tgtgaaaata
      301 tttatctcaa tcacaaaaag aattcaaatg tggaggctag taaattgttg ggaagtaaag
      361 ctattttaat aataaaatgt atggttttgc tcaagctgtt ttataaaaat aatcgctcga
      421 taaatgcaga tgctattttt cgcaatatgt aaaatatatt gccatcatat tttgcctatg
      481 ataaaacaat tttaatttct ttaatacttt gaaaaatatc gttaatgcaa atcgaaggtg
      541 cgtataaggt cacggttagg cgttccgcta tggcacccaa tacaaccgct acctttattg
      601 gcggcactat atgttactgt tccatcaggt ttaaactcgg cccaaaccca tccacttccg
      661 gcattggcat tcgaagcatc ttttttcata actgcaaatt ggtcgagggt ggtgccaaca
      721 aataattctt ttacaataat ggaaccggta ggaaaagaat tgccaacaag cgcagctttt
      781 gcaatattat taaatcgcaa cttgaaagat ccatgtgggc taggtgatac tccgctgagc
      841 aaagttccat tctgataata agtgtagccc gatgaattta cttcatcaat cagtgcttga
      901 ttggcatctt cttcggcttt gtcttttttg cagctgctta caattaatgc gaatactgat
      961 atcataaaaa cagcgtgaat tatttttttc gctttcataa tttttattaa ttaattgcaa
     1021 gtaacgcatt tattataaaa tcgcaatgtc ttgaagatga cattattcaa tttaggtcac
     1081 aacttttaag tcgaaaatct ttaagcaata ttgcaattgc ttttaaaaag gaggaaaata
     1141 aattaccgcc atactcactc aaaaaaccat ttctttgctg cacaaaatat gacacaacaa
     1201 aaaagcggtt tcgtttcatc gatgagaata aataactccg tactaagtac ttttaccggt
     1261 gtattgctat gggcagcgtg gccggcaaat ggcgtggctc cactattgtg ggtggcattt
     1321 atccctttgc ttattatcga gtggaggttt tataaccact ccgatcaaaa tggcaaatct
     1381 ccatttcgtt atttctattt cacatttgtg ttgttcaatt tgtttaccac gtggtgggtg
     1441 tggaatgcca gtgcatttgg agtgataatg gccattgttt taaattcact ttttatggca
     1501 actgtgtttt ggttgtttta taaatgccat aaaattttga agagtgattt ggcatatttt
     1561 cttttacccg tgttttggtt atcgttcgag tatatgcaca tggattggga tttatcgtgg
     1621 ccttggctaa cccttgccaa tggctgggca gcatggtaca agtgtgtgca atggtatgag
     1681 tttacaggct ttagcggagg taccatctgg acttgggcag ccaacatttt tttattcctg
     1741 gcaataaaac atcatgcaca accactggca aaaaaatatt tccgcttagc acttagtgta
     1801 attatttttc ctcttgtttt atcatacacg atttatttca cttacaccga aaaaattaat
     1861 cctgtaaata ttgttgtagc acaacccaat atcgacccat acaacgaaaa atttgtgggc
     1921 gataattttt cacatatcga aatgatgttg aagctggcct caactaaaat tgatacaact
     1981 acagattatt atgtaacgcc cgaaacggct attgcatcgg gtatttggga agaacaatta
     2041 tataaccacc gtaccatacg gttaatgcaa aatcttgtaa agcaatatcc gcaacttagt
     2101 attgttacgg gtgcagccac cgataagttt tatcgtaaag gagaaaataa atctgtaacc
     2161 gcacgcaagt tttccgaaac ggatgattac tatgattcgt ataacactgc attgcaaatt
     2221 gatgctaccg atagcattca gatataccat aaaagcaaat tagtgccggg tgtagagaag
     2281 atgcctttcg aatttatttt caaacacatg gaacagtttg ccatcgacct cggtggagct
     2341 tcgggcagcc tgggaaatca aaatgatgta agtgtattca aaacgcaaaa gcacaccatt
     2401 gcccctgtta tttgttacga gtctatttac ggaaagtatg taggtaaata tgtacgcaaa
     2461 ggtgccgact tgatttttat aattaccaac gatggatggt gggggaatac tcccggctac
     2521 cgtcagcatt tgcagtatgc acgcttgcgc gcaattgaaa tgcgcagaag cattgcacgt
     2581 agtgccaata caggcatctc atgttttatt aatcaaaaag gcgacattat tcagcaagca
     2641 caatggtggc agacagatgt gttgaaacaa actataaacg ccaatacaac catgaccgta
     2701 tacgcacgca ttggcgatat tattgcaatt gtttcttgct tgttgagctt gattgctttg
     2761 ttatggtgtg gtgctattac ttttagaaat aaagctaact aattaggcac aattagtttt
     2821 ctattctttg acgagtatta cactttcctc aaataccccc acaaaataac agcatagaaa
     2881 aaaataaaaa atgtaatgcc ggcttgagtt tctaatgtgt cttcgttgaa aaatgagagt
     2941 aatgaaatgg aaaagaaaac aagaaaaata aaatgacgtt gtgcgtttag ttttataaca
     3001 ggatagagca gagcaaaaat aaaaagtgct aaaccaacca taccaaacga aacagcaata
     3061 gccatatatt gattgtgcga tcgcaatcgc cacttttgcc ctagtttcga atttgttgtg
     3121 ttatagtaat tttcaaatgc aattttattg tcacctgtgc ctacaccaaa aagcaaattg
     3181 tttttcaaga tgtaccacga gcctttgtta tattcaatgc gctgtgctat ggagaagcca
     3241 ctggcgttgc cggttttttt gtattggttt agttcgtaag caatttcaac aaagcgcaat
     3301 tgcaacccat tcatatttac atgagtgcag ttagcaatgc cttgttccac ggcttttaaa
     3361 tcatcgttgc taagcgcgta tacacctgca ctatctttgt gcaaattttt cgatgttaag
     3421 tatcgaatca acgtataggg taatggttgc tttctattat cgaggctatc gaacgaaaat
     3481 ttgctgcgct cgttccacga ctttcgcaat tcgttccagc aaatatacca gccaagtcgg
     3541 tgtccatttt cgcacccttt gtatgattga acatgttgat agaggtttcc gttgggcgta
     3601 tatttttcgg tagggggact ttcgttttca ttacaatttc ccaatgcatt catttgattt
     3661 atgaaataaa aactactcca ggcaagtgtg catactgcca acacaaacat caaagacaat
     3721 ggaatatatt ttttacttct taccagctga attcctgcta atacaaacaa tatcacacac
     3781 ataataaaaa gtccagtcag cgattcgaga ataaaaagaa aaataatcaa ccatattgcc
     3841 gcagccgtcc atcctgcttt ttttaaataa tactttgata aaaaagcaaa atatgacgaa
     3901 ataaaaattg ccaacaccac cat
//
LOCUS       DAORQA010000167         3918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5615_length_3918_cov_4.850116, whole genome shotgun sequence.
ACCESSION   DAORQA010000167 DAORQA010000000
VERSION     DAORQA010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5615_length_3918_cov_4.850116"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2064
                     /locus_tag="PK736_07555"
     CDS             <1..>2064
                     /locus_tag="PK736_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRC33284.1"
                     /translation="PNLILDNWMGKLGAIIGHVFVFKGFGICSLAFCVVMFFAGFGFL
                     FNQRIFSWSKIIGYTLFTTVIGSVTFGFLLQNKSDLLFIAGQYGYEVSKWLQSSLGLA
                     GEALLLLFAFAGLTIHVFDIKFAYNKTPLKDEDTKITAPIIADAPPLKQEQIIEHKVS
                     SPVEFNVKKVEPKPTANNILKVPELEIVDEIKPELELEMNVDEIELDENEIENDVIVA
                     ESDANDDEVPMTIEQTNDDNELTEINESNILEKLGAYDPTLDLSTYKFPELDLLEGYL
                     NQKNKLTPEELKAELETNKNKIVETLGHYNIGIEKIKATVGPTVTLFEIVPQAGVRIS
                     KIKNLEDDIALSLSALGIRIIAPIPGKGTIGIEVPNQTPEIVSMRSVIASEKFQNSDY
                     DLPIVLGKTISNEVYVCDLAKMPHLLMAGATGQGKSVGLNAVLVSLLYKKHPSQVKFI
                     LVDPKKVELTLFKKIERHFLAKLPGEDESIITDTKKVVNTLNSLCIEMDNRYELLKEA
                     QVRNLKEYNQKFTQRRLNPNNGHRFLPYIVLVVDEFADLIMTAGREVETPIARLAQLA
                     RAIGIHLIIATQRPSVNIITGTIKANFPARIAFRVTSKIDSRTILDAGGADQLIGRGD
                     MLLSTGADLIRIQCAFVDTPEVEKITEYIGSQRGYSSAFLLPEYVGPEGAEGGSPSDF
                     TAADR"
     assembly_gap    2065..2133
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2134..2289
                     /locus_tag="PK736_07560"
     CDS             <2134..2289
                     /locus_tag="PK736_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRC33285.1"
                     /translation="LQRRLKLGYNRAGRLVDQLEAAGIIGPFEGSKAREVRVKTEAEL
                     HELLLNQ"
     gene            2384..3775
                     /locus_tag="PK736_07565"
     CDS             2384..3775
                     /locus_tag="PK736_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRC33286.1"
                     /translation="MKIILSHGYFLKEDPRELEIMKPYVPLGILYISAWLEKNGFEND
                     VYDSTFSSFEKFCENLLTKKPQLLCLYTNLMTKLNILKIIDFVKNEPLLSNTKIILGG
                     PEVRNHDEAFLNYGAHIIVVGEGEQTMLDICLHFIDGQFNISEIESICYKEDGKIIRT
                     KERSKIRSIDELPFPNRHKVDLQKYFDAWKSKHGISSISLSTMRGCPYTCNWCSRAVY
                     GQSYRRRSPKLVVDEIIEVQKNYKFDSIWFVDDVFTISHKWLAEFNHEINSRQVKINF
                     ECITRADRLTEEALLMLKESGCMRVWIGAESGSQKIIDAMDRRVDVKQVRRMLIAAKK
                     MGIQTGTFIMLGYPGENIEDIEETIKHLTESDPDLYTITIAYPIKGTQLYNQTESNFI
                     NQLPWHNSTDRQIDFKRTHHKKFYEMAIRLIYNEVKLHKLKKENFGQTKNMMVTYAKI
                     KAARLLMKTYQLF"
BASE COUNT         1304 a          677 c          719 g         1149 t
ORIGIN      
        1 accccaatct tattcttgat aattggatgg gaaaacttgg cgccatcata ggtcatgttt
       61 ttgtatttaa aggcttcggt atttgttcgc ttgcattttg tgtggtgatg ttttttgccg
      121 ggtttggttt tttattcaat caacgaattt tttcatggtc gaaaattatt ggttacactt
      181 tgttcactac agttattggt tctgttactt ttggttttct tttgcaaaat aaaagcgatt
      241 tgctttttat tgccgggcaa tatggttatg aagttagcaa gtggttgcaa tcttcacttg
      301 gtttagctgg cgaggcttta ttgttgcttt ttgcttttgc aggtcttact atccatgttt
      361 tcgatattaa atttgcttat aacaaaacgc cattaaagga cgaagacacc aaaataaccg
      421 ctcccataat tgccgatgcg ccaccactga aacaagagca aattatagag cataaagtaa
      481 gcagccctgt cgaattcaat gtgaaaaaag ttgagcccaa accaactgca aataacattc
      541 ttaaagttcc cgagttggaa attgtagacg aaataaaacc tgagctcgaa ttggaaatga
      601 atgtggatga aatagaattg gacgaaaacg aaattgaaaa tgatgtaatt gttgctgaat
      661 cagatgctaa cgatgacgaa gtaccaatga ctattgaaca aacaaatgac gataatgaac
      721 ttacggaaat aaatgaatcc aatattttag aaaaattagg agcctatgat cctacactcg
      781 atttatctac ttacaaattt cccgaactcg atttgcttga aggatattta aatcaaaaaa
      841 ataaattaac acccgaagaa ttaaaagccg aacttgaaac aaacaaaaat aaaattgtag
      901 aaacattagg ccactacaac atcggtatcg aaaaaataaa agcaaccgtt ggcccaactg
      961 ttacattgtt cgaaatagtg ccacaggccg gtgtacgtat atccaaaata aaaaacctcg
     1021 aagatgatat cgcattaagt ctttcggcat tgggcattcg catcattgct cctattccgg
     1081 gcaaaggaac aattggtatc gaggtgccga atcaaactcc cgaaatagtt tcgatgcgct
     1141 cagtaattgc ttccgaaaaa tttcaaaact ccgattatga tttaccaatc gtattaggaa
     1201 aaactatttc gaacgaagta tatgtttgcg accttgcaaa gatgcctcac ttgcttatgg
     1261 ctggtgcaac cggacaggga aaatcggtag gattaaatgc tgtgctggtt tctcttcttt
     1321 ataaaaaaca tccttcgcaa gtaaaattta ttttggtcga tcctaaaaaa gtagagttaa
     1381 cattgttcaa aaaaatcgaa cgtcattttc ttgcaaaact tccgggcgaa gatgaatcaa
     1441 taatcaccga cactaaaaaa gttgtgaata ccttgaactc gctttgtatt gaaatggata
     1501 atagatatga gttgcttaaa gaagcacaag tgagaaattt gaaagagtac aatcaaaaat
     1561 ttacacagcg tagattgaat ccaaataatg gtcaccgctt tttaccttat attgttttag
     1621 tagttgatga atttgccgat ttaattatga ccgcaggtcg cgaagtggaa acgcctatag
     1681 ctcgccttgc acagcttgca cgcgctattg gtatacattt aataatagca actcaacgtc
     1741 cttcggtaaa tattattacc ggaactataa aagcaaattt ccctgcacgt atcgcatttc
     1801 gtgttacatc gaaaatcgat tcccgcacca ttctcgatgc cggtggtgcc gaccaactta
     1861 taggccgtgg cgatatgtta ctttcaaccg gtgctgattt aattcgtata caatgtgcat
     1921 ttgttgatac gcccgaagta gaaaaaataa ccgaatatat tggttcgcaa cgaggatatt
     1981 cttctgcatt tttattgccg gaatacgttg gccccgaagg tgcagaaggt ggaagcccca
     2041 gcgactttac tgctgccgat agagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncttcagc gaagattaaa acttggttat
     2161 aaccgtgccg gccgcttagt cgaccagtta gaagccgcag gaatcatagg accatttgaa
     2221 ggaagcaaag cgcgcgaggt acgagtaaaa accgaagccg agttgcatga gctactgtta
     2281 aatcaataaa accaattacc tgtttcatat gtttcacaat tagttttaag ctataaaaaa
     2341 ttgaagtcta atttttgaaa agcttttagc tacttttacc actatgaaaa taattttgtc
     2401 gcatggatat tttttaaaag aagatcctcg tgagcttgaa ataatgaaac cttatgtgcc
     2461 attaggaata ttatacattt ctgcctggct cgaaaaaaat ggtttcgaaa atgatgtgta
     2521 cgattctact ttttcttcct tcgaaaaatt ctgcgaaaat ttattgacta agaaaccaca
     2581 gttactttgt ctctacacca atttaatgac aaagttgaac attttaaaaa ttatagactt
     2641 tgtaaaaaat gaacccttac tttcaaatac aaaaattatt cttggtggcc ccgaagtgcg
     2701 aaatcatgat gaggcttttt tgaattatgg cgctcacatt attgttgttg gcgaaggcga
     2761 acaaactatg ctcgatatat gtttgcattt tatcgatggg caatttaata tttcggaaat
     2821 agaaagtatt tgctacaaag aagatggaaa aataatcaga acaaaggagc ggtccaaaat
     2881 tcgttcaatt gatgaacttc cttttccaaa ccgacacaaa gttgatttgc aaaaatattt
     2941 cgatgcctgg aaatcgaaac atggcataag ttcaatttcg ctttctacca tgcgtggttg
     3001 cccatacact tgtaactggt gcagccgtgc tgtttatgga caaagttatc gaaggcgatc
     3061 accaaaattg gtagtcgatg aaattattga agtgcaaaaa aattataaat tcgattctat
     3121 ttggtttgtt gatgatgttt ttacaatcag tcacaaatgg ctggctgagt ttaatcacga
     3181 aataaattca cgtcaagtga aaattaattt tgaatgcatc actcgtgcgg atagattgac
     3241 agaagaagca ctcttaatgc tcaaagaaag tggctgtatg cgtgtttgga ttggtgcaga
     3301 aagtgggtca caaaaaataa ttgacgccat ggatcgcaga gttgatgtaa agcaagtaag
     3361 gcgaatgctt attgcagcaa aaaaaatggg tattcaaaca ggcactttta ttatgctagg
     3421 ttatccggga gaaaacattg aagatattga agaaaccata aaacatttaa cggaatccga
     3481 tcccgattta tatacaataa ctattgcata tccaataaag ggaacacaat tatacaacca
     3541 aacagagtcg aactttatca atcagctacc atggcataat tcaaccgata ggcaaatcga
     3601 ttttaaaaga acccatcata aaaagtttta tgaaatggcc atcaggctaa tttataacga
     3661 agtgaaactg cataaattga aaaaagaaaa ctttggtcaa acaaaaaata tgatggtaac
     3721 ttatgccaaa attaaagctg ctcgcttatt aatgaaaaca tatcagctgt tttgaaaatt
     3781 gttttttagt ttaagaacaa ataactattt cctacttatc aaaagcccac ataaacccaa
     3841 ttctaaccat tcttctttcc atgggataat ggggagtatc aaaaaattgt tcactcatta
     3901 atccgttcaa tatattat
//
LOCUS       DAORQA010000168         3900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5654_length_3900_cov_6.917815, whole genome shotgun sequence.
ACCESSION   DAORQA010000168 DAORQA010000000
VERSION     DAORQA010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5654_length_3900_cov_6.917815"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2326
                     /locus_tag="PK736_07570"
     CDS             <1..2326
                     /locus_tag="PK736_07570"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC33287.1"
                     /translation="AEAYYRKGDYNQASVLFLEYQKTTPSLTRDDLYKIGYTYYKVEK
                     YNDAILNFQKVVNVNDTMAQKAYYHLGDCFLKTKNKQSARNAFQFAAKSDFDAAITED
                     ALFNFGKLSFELGFQPQAITTFTQYVKNYPTSHNKDICNEYLAKLYLQTKNYKEALAL
                     LESLKDKNPTAKEAFQKVAYYRGNELYNDGDYSKAIGLYEKAIVNNVDNNMAAYAIYW
                     KAEALYKQQKYEAAIKQFRTFVFYPSSVNIANHDNALYDLGYCHFKLKQYTVAGDWFR
                     KFIAAITTTADARAIDARVRIADVGFISGNYDVAREFYTAAITAKSSATDYCLFQKGM
                     IDGIQDNHEAKIFAMQNIQESFPKSNYYDDAIFESGDAYFINGDNENAVKQFETIINS
                     KKGSSYISKAYLKLGLIYFNKGENKKAYDTFKQVVETNPRSNESMEALMQMKNIAVKE
                     GNPDDFIAYMSKLPLPKASDGAQDSLYYETAENFLEKQEYEKASAAFGTYISKFPQGN
                     FALNANFYNAECLVKLNKFSEALIIFERIIDGPKNLFTEKSLFRAAAINFKNKEYSKA
                     LVQFERLEQTAEYKDNIIASYAGQMRCNYYLDKFDNAIIAAQKVLATDKLQPELANEA
                     HLIYARCAIAKDDIANAQREYKEVEKIGNSERAAEARYNLALIQYKQKKYKEAEKGAF
                     LVIRQLPSYDYWIAKSFILLGDNYIALADTFQAKSTLKSVLDNYKPSQSDKDDILKIA
                     QEKYDTIIADENTIFRKPQNDELEFDTDSLNNKQ"
     gene            2338..>3900
                     /locus_tag="PK736_07575"
     CDS             2338..>3900
                     /locus_tag="PK736_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC33288.1"
                     /translation="MKQVIKKINKSYTVMVKYMTASAIILFCGISICKAQKNEKDLGD
                     ETIVIDKGYKPMLAEGEKISESPKNDTTLVQTPVMKYEVANVQSETKYIINPVKPLRV
                     RDEYIKELYRGFVKAGYGFPSLPYMEIAYNSLRSKDFDAGVHFRHLSSKGTIKEFGYP
                     GFSNNTLNLQATKYLEHADLKVKLDYDRDLYHFYGYDAEEIFFEKKETRHIFNTLNTS
                     LEYTGRTEDADDFQYKAKVAFYNSKNNLKVTENNFNVNAYLGKNIGKGNVDGIIVASI
                     NKIEQELQSQTNTLVSISPRYNISLYPWKLSAGININVLSEFDQTEVFVFPYARFNYQ
                     LIENKLSVLGEVGNALKQNNFLTLARENPFIDNENIYHATHTLFTGTFGANAMLGKGV
                     QFHASVKYDNNIGDVFFINKETVPVKFSTIYLNDQVVRIHGDISYDPAKKLFASVSID
                     NYNYTFDNDSLMPLYKPAVRAGVALGYNLRDKITVRTDLYINSKANYFDHELNDYAKL
                     KSWVDVNLYAEYR"
BASE COUNT         1377 a          683 c          757 g         1083 t
ORIGIN      
        1 ggccgaggca tattaccgta aaggggatta taatcaggca tcggttttgt ttttggaata
       61 tcaaaaaaca acaccatcac tcacccgcga tgatttgtac aaaataggtt atacatatta
      121 caaagttgaa aagtacaacg atgcaatttt gaattttcaa aaagtagtaa acgtaaacga
      181 taccatggcg caaaaagcat attaccattt gggcgattgc tttttgaaaa caaaaaataa
      241 acaaagtgca cgcaatgcat ttcaatttgc ggccaagtcc gatttcgatg ctgcaattac
      301 cgaagatgct ttgttcaatt ttggtaagct atcattcgag cttggctttc aaccacaagc
      361 tataactacg tttacacagt acgttaaaaa ttatcccacc tcacacaaca aggacatctg
      421 caacgaatat cttgctaaat tgtatttgca aacaaaaaat tataaagagg cgctcgcact
      481 tttagagtca ctcaaagata aaaaccctac tgcaaaagag gcgtttcaaa aggtggctta
      541 ttacagaggc aatgaattgt ataacgatgg tgattattca aaggctattg gtttgtacga
      601 aaaagcaatt gtaaataatg tagataacaa catggctgca tatgccattt actggaaagc
      661 cgaagcgtta tacaaacaac agaaatatga ggcagccata aagcaattcc gcactttcgt
      721 tttttatccc tcatcggtaa atattgcaaa tcacgacaat gctttgtatg atttgggata
      781 ctgtcatttt aaacttaagc aatacacagt ggcaggcgat tggtttcgga aatttattgc
      841 tgctataact accacggccg atgcacgtgc aattgatgcg cgagtgcgta ttgcagatgt
      901 aggttttatt tcgggcaact acgatgtggc acgcgagttt tatactgctg ctattactgc
      961 caaatcatcg gcaacagatt attgcctgtt ccaaaaggga atgatagatg gaatacaaga
     1021 caaccacgaa gccaaaattt ttgccatgca aaatattcag gaatcgtttc caaagagtaa
     1081 ttactatgat gatgctattt ttgaaagtgg cgatgcatat ttcatcaatg gtgataatga
     1141 aaacgctgta aagcaatttg aaacaataat taattccaag aaaggaagtt cttatataag
     1201 caaagcgtat ttaaaactgg gattgattta ttttaataaa ggcgaaaaca aaaaagctta
     1261 cgacactttt aagcaagtag tagaaaccaa tccgaggtcg aacgaatcga tggaagcgct
     1321 tatgcaaatg aaaaatattg ctgtgaaaga aggcaacccc gatgatttta tagcctatat
     1381 gagcaagttg ccattgccca aagccagcga tggagcgcag gattcattgt attatgaaac
     1441 agcagaaaac tttctcgaaa agcaagaata cgaaaaagct tctgccgcat tcggcacgta
     1501 tatatcaaaa ttccctcaag gcaattttgc gttgaatgca aatttttata atgccgaatg
     1561 ccttgttaag ctcaacaaat ttagcgaagc attaattatt tttgaaagaa taattgacgg
     1621 acctaaaaat ttatttaccg aaaaatcttt gtttcgtgca gcagcaataa attttaagaa
     1681 caaagaatat tcaaaagcat tggtgcagtt tgaacgattg gaacaaacag cagaatacaa
     1741 ggataacatt atagcatcgt atgccggaca aatgcgctgc aactattatc tcgataaatt
     1801 tgataacgcc attatagctg cacaaaaagt attggctacc gataaacttc agcctgaatt
     1861 agcaaacgag gctcacctta tttatgcgcg atgtgcaatt gccaaagatg atattgccaa
     1921 tgcacagcga gaatataaag aagtagaaaa aattggcaat agcgagcgtg cagccgaagc
     1981 acgctataat ttagcattga tacagtacaa acaaaaaaaa tataaggaag ctgaaaaagg
     2041 agcatttctg gtaatacgcc aattgcccag ttatgattac tggatagcca aaagttttat
     2101 cctgcttggc gataattaca tcgcacttgc tgatactttt caggctaagt ctactttgaa
     2161 aagtgtgctc gataattata aaccttcaca atctgataag gatgatatac taaagattgc
     2221 acaggaaaaa tacgacacta tcatagccga tgaaaatacc atttttcgca agccgcagaa
     2281 tgatgaactc gaattcgaca ccgacagttt gaataacaaa caataatagt aacgaaaatg
     2341 aaacaagtga ttaagaaaat aaacaaatcg tatacggtca tggttaagta tatgacggct
     2401 tcggctatta ttttattttg tggcatttca atatgcaaag ctcagaaaaa cgagaaggac
     2461 cttggtgacg aaaccatagt gatagacaaa ggatacaaac ccatgttggc cgaaggagaa
     2521 aaaatcagtg agtcgccaaa gaacgatacc acattggtgc aaactcctgt gatgaaatat
     2581 gaagtagcaa atgtgcaaag cgaaacaaaa tatataatta atcctgttaa gcctttgcgt
     2641 gtacgcgatg aatatattaa agaattgtac cgtggttttg tgaaagccgg ctatggcttt
     2701 ccatcgttgc catacatgga gatagcttac aatagcctgc gttcaaagga ttttgatgcc
     2761 ggtgtgcact ttcgccattt atcatcgaaa ggtactataa aggaatttgg ttatcccggg
     2821 tttagcaaca acacgctcaa cttgcaggca acaaaatatc tggagcatgc cgatcttaaa
     2881 gtgaaacttg attatgatcg cgacctctat catttctatg gttatgatgc cgaagaaata
     2941 tttttcgaaa aaaaggaaac tcgacacatt tttaatacgc tcaacaccag tttagaatat
     3001 actggaagaa cagaggatgc cgatgatttt caatacaaag caaaagtggc attttacaat
     3061 tcgaaaaata atttaaaagt gactgaaaat aattttaatg taaatgccta tttgggaaaa
     3121 aacattggca agggcaatgt tgatggaata attgttgcat ctattaataa aatcgaacag
     3181 gaacttcaat ctcaaacaaa tacgctggtg agtatttcgc cccggtataa tatttcattg
     3241 tatccctgga aactttcggc aggtatcaat attaatgtgc tgagtgagtt tgatcaaaca
     3301 gaagtattcg tttttccata tgcccgattt aattatcaat tgattgagaa caaattgagt
     3361 gtgttgggcg aagtgggtaa tgcattaaag caaaacaatt tcttaacctt agcgcgcgaa
     3421 aaccctttta tagataacga aaatatatat catgctacac acactctatt tacaggaact
     3481 tttggtgcaa atgccatgct tggcaaaggg gtgcagtttc atgcaagtgt aaagtacgac
     3541 aataatatag gtgatgtgtt ttttataaat aaagaaaccg ttcctgtaaa gttcagcacc
     3601 atctatctca atgatcaggt tgtaaggatt catggagata tttcttacga cccggcaaaa
     3661 aaactatttg catctgttag catcgacaac tacaattata ctttcgataa cgatagtttg
     3721 atgccattat acaaaccggc agttcgtgca ggtgttgcat tagggtataa ccttcgcgat
     3781 aaaattactg tgcgcacaga tttatatatc aactcgaagg caaattattt cgaccatgaa
     3841 ctgaacgatt atgccaaatt aaaatcgtgg gtcgatgtga atttgtatgc ggaatatcgt
//
LOCUS       DAORQA010000169         3888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5677_length_3888_cov_9.216541, whole genome shotgun sequence.
ACCESSION   DAORQA010000169 DAORQA010000000
VERSION     DAORQA010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5677_length_3888_cov_9.216541"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..429
                     /locus_tag="PK736_07580"
     CDS             22..429
                     /locus_tag="PK736_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33289.1"
                     /translation="MKYNIGTKEKPMALKTPPLSSEYTMHVDEKDGKEVLVCTVGKTV
                     LLYNMNCLSDLHAMLKKHGEWMELGSADEQKPAKEGTVEAWGRSEKNPAGGWYGLKKG
                     LRGRFGMYIPPLMEALGLAEVTHDAKGNKMRAK"
     gene            531..866
                     /locus_tag="PK736_07585"
     CDS             531..866
                     /locus_tag="PK736_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33290.1"
                     /translation="MKIFISLLSLLNGGYMLIDGLYVIVNGKYIGPQKPGPWANLFYK
                     LNIDVYKLGPMFILFGIAWFAFLFALWTNNTKAYIMGMAISVLTLWYLPVGTVISIIV
                     MALIFFMLK"
     gene            1224..2090
                     /locus_tag="PK736_07590"
     CDS             1224..2090
                     /locus_tag="PK736_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33291.1"
                     /translation="MKIIHIMSIAACLGVLQSCNIINPEEPVPAYIAIDKLTLQADYT
                     TQGSSSSAFSDAWLYVDNQSIGGFEMPTIVPVIADEGNHLINIAAGVKASGFENIRSL
                     LALFTFYDTTVYLKPGVVSYFSPVLKYKEAAKFDYIEDFDDGGIEFIKYNNSSDTTFY
                     ATNANPFEGDYSAIAWLDNTRKSMAVVSKDAYVIDKNRLNIIEVNYKCSTSFNIGVSK
                     NIFGGTSEPIPFLTLYPTSVWKKIYIDLSPIIGANVNFNAINYKLYISAFLDADKTSG
                     DIQIDNLKLVHL"
     gene            2090..3511
                     /locus_tag="PK736_07595"
     CDS             2090..3511
                     /locus_tag="PK736_07595"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRC33292.1"
                     /translation="MVVSRRILTIKYILADFVAALAAWGLFYSYRKLYIESSKFGHSI
                     AINVDAKFIVGLLIIPLFWVAIYALVGSYSNVMRRSRLRELGQTAYLSVIGVLILFFT
                     LLLDDTIVNYKSYYSTFFTLLCLHFIFTATLRLILSTGVAHRIRNRQLGFDTLMVGSN
                     QNAVNLFNEFESAKQSYGNFFKGFVHVDDSNGKFFKNSLPHLGGVNDLREIIKEHKIE
                     EVIIAIESNEHEKVGKIINELEDTNVVIKIIPDMYDILTGTVKMNAIFGALLIEINPY
                     LMPVWQQSFKRLFDIVLSFITLIILSPVYIILSILVKTTSHGPIFFSQERIGLHGNPF
                     IIYKFRTMYQDAEHQGPQLSKENDTRITKLGKWMRKIRLDEIPQFFNVLMGDMSLVGP
                     RPERQFYIDQIMLRAPHYRHLQKVRPGITSWGQVKFGYAENVEQMIERLKYDIIYIEN
                     MSIALDIKIMFYTLKTIVEGRGK"
BASE COUNT         1263 a          669 c          726 g         1230 t
ORIGIN      
        1 ttaaatctaa taaattaaat tatgaaatat aatattggaa caaaagaaaa gccaatggca
       61 ttaaaaacgc caccattatc atcggagtat acgatgcatg ttgacgaaaa agatggcaaa
      121 gaagtattgg tatgtactgt aggtaaaacg gtgctacttt ataacatgaa ctgtctcagc
      181 gatttgcatg ctatgttaaa gaagcatggc gaatggatgg aactcggcag tgccgatgaa
      241 cagaaacccg ccaaagaagg cactgttgaa gcatggggcc gctctgaaaa aaaccctgcc
      301 ggtggttggt atggcttaaa aaaaggtctg cgtggcagat ttggaatgta cattccgcca
      361 ctgatggaag cattaggact tgccgaagtt acacatgatg caaaaggcaa taagatgcgc
      421 gcaaaataaa atcgcaaaat agaaaacgaa taaattttta ttcccggaca tgccatctaa
      481 atgcgtaaaa taattctctt tttattcgat agatacacgc acaaatcatt atgaaaatat
      541 tcatatcatt attaagttta ttgaatggag gatatatgtt gatagatggc ttatatgtta
      601 ttgtaaatgg aaaatatatt ggcccacaaa aaccgggacc atgggcaaat ttattttata
      661 aattaaatat tgatgtttac aaactcggcc ctatgtttat tttgtttgga attgcctggt
      721 ttgcttttct ttttgctttg tggacaaaca atacaaaagc ttacataatg ggcatggcta
      781 tttcagttct tactttgtgg taccttccag ttggtaccgt tatttctatc attgtaatgg
      841 cacttatatt ttttatgtta aaatagaaat gttgaaatcg atttttttat tgcgaaccat
      901 agttgtttta caataacatt ctgctgtaag taaatagcat aataataagt aagcagcatc
      961 ttcaacagaa tgcggaatat tagtttaaaa taaactacat attcattatc ctatttttaa
     1021 tgttaaagcc aataaaacat caaagtttat aagataaaca tgtatcatcg aaattatacc
     1081 gaaacaactt aagttttgca aaaacaagct attgaagttg ttcggcataa ctaagagtac
     1141 gttatatttt cattcgaaaa tctaactact cttagtataa atgatttgtt tacatttgcg
     1201 ctccaataat aaaaaaggtc gatttgaaaa taattcacat aatgagcatt gccgcatgcc
     1261 tgggtgtttt gcagagttgc aatattatca atcccgaaga gcctgtgccg gcatatatcg
     1321 ccatcgacaa attaacgcta caggccgact atacaaccca gggctcatct tcgagtgcct
     1381 ttagtgatgc ttggctttat gttgacaatc aatccatcgg aggtttcgaa atgccaacca
     1441 tagtgcctgt aattgcggac gagggcaacc acctcattaa tattgctgca ggagtaaagg
     1501 caagtggctt cgaaaacatt cgttcgttgc tggcattgtt cacattttac gataccacag
     1561 tttatttaaa acctggagtg gtaagctatt tttcaccggt attgaaatat aaggaagcgg
     1621 caaagttcga ttacattgaa gattttgacg atggtgggat tgaatttata aaatataaca
     1681 acagcagcga cactactttc tatgcaacaa atgccaatcc attcgagggc gattattcgg
     1741 caattgcctg gttggataat acccgaaaaa gcatggcggt ggtgagcaaa gatgcttatg
     1801 tgattgacaa aaaccgactc aatattatag aagtaaatta caaatgcagt acttcgttta
     1861 atataggagt atcgaaaaat atatttggcg gcacaagcga gcctatacct tttcttacac
     1921 tataccctac ttcggtgtgg aaaaaaatct atatcgattt atcgccaata attggtgcca
     1981 atgtaaattt caatgccatc aattacaaac tttatataag tgcttttctc gatgccgaca
     2041 aaacaagtgg cgatattcag attgacaatt taaaattggt gcatttgtaa tggtcgtttc
     2101 acgaagaata cttacaataa aatatatcct tgccgatttt gttgctgcac ttgctgcatg
     2161 gggtttgttt tacagctacc gcaagttata cattgagtcg tcaaaatttg gacattctat
     2221 tgccattaat gttgatgcaa aatttattgt tggcttactt atcattcctt tattttgggt
     2281 tgctatatat gcattggttg gcagctacag caatgttatg cgcagatcgc gcctccgcga
     2341 attagggcaa acagcatacc tttcggttat aggagtgctg atattatttt ttactctttt
     2401 gctcgatgat acaatagtca attacaaaag ttattattct acatttttta ctttgctgtg
     2461 cttacatttt atttttacag caacattgcg tcttatttta tcaaccggtg tagcacatcg
     2521 catacgcaat cgtcaattgg gtttcgatac tttgatggta gggagcaatc aaaatgcggt
     2581 aaaccttttc aacgagtttg aaagtgccaa acagagctat ggaaattttt tcaaaggctt
     2641 tgtgcatgtg gatgatagca atggaaaatt ttttaaaaac tcactgcccc atcttggcgg
     2701 tgtaaatgat ttacgcgaga taataaagga acataaaatt gaagaagtaa ttattgccat
     2761 agaatcgaac gaacatgaga aagtagggaa aataattaat gaacttgaag acaccaacgt
     2821 ggtgataaaa attattcccg atatgtacga catacttacg ggtacggtaa aaatgaacgc
     2881 catattcgga gctttactta tcgaaatcaa tccttacctg atgccggtgt ggcaacaaag
     2941 ttttaaacgg ttgttcgaca ttgtactttc gtttattacg ctcatcattc tttctccggt
     3001 ttatataata ctaagcatac ttgtaaaaac aacttcgcat gggccaatat ttttttcgca
     3061 ggaacgcatt ggcttacatg gcaatccatt tatcatttat aaatttcgta caatgtatca
     3121 agatgcagaa catcagggac cgcaattgtc gaaagaaaat gatactcgca ttacaaaact
     3181 tggcaagtgg atgcgtaaaa ttcggctgga cgaaatacca caatttttta atgtactcat
     3241 gggcgatatg agccttgttg gcccaaggcc ggagcggcaa ttttatatcg accagataat
     3301 gttgcgtgcc ccacattaca ggcatctaca aaaagtgcga cccggtatta catcgtgggg
     3361 ccaggtaaag tttggctatg ccgaaaacgt ggagcaaatg atagaacgac ttaaatacga
     3421 tatcatctat atcgaaaaca tgtccattgc ccttgatatt aaaataatgt tttacaccct
     3481 aaaaactatt gtagaaggac gaggtaaata gtgttttata acaatacttc agcactaata
     3541 ctcccataaa tatttttcaa ctaacttttt tctttttgaa ataatttgca attgatttgt
     3601 tttacaaaaa ttgttttgtg gtgatttctc atcatgcctt tagctgtttt taaatatttg
     3661 attgttatac aaattgcgaa tttcagggct ttggcgcaat gtctttaaat aaaccctttt
     3721 gcaataaaaa ttctataaac cgtagtttag ttaatcgcta ttttgaaata aacttgccat
     3781 tataaaaaac gctaaaacaa aaaagatgat ttcaacttat agatgtcccg tagggcatac
     3841 gcgtcaactt tagctcgaat cattcatttg ctcgtgatgg tttttcct
//
LOCUS       DAORQA010000170         3855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5749_length_3855_cov_8.069737, whole genome shotgun sequence.
ACCESSION   DAORQA010000170 DAORQA010000000
VERSION     DAORQA010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5749_length_3855_cov_8.069737"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..3535
                     /locus_tag="PK736_07600"
     CDS             35..3535
                     /locus_tag="PK736_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRC33293.1"
                     /translation="MFSFIYCCSTKNDDGKTENLSATKPLFNLLAVDKTGIDFNNTLN
                     ETATQNIYAYQYFYNGAGVAAGDINNDGLVDVFFAANQGSCKLYINKGDLKFQDITLQ
                     SKIATTGWCTGVAMADVNGDGWLDIYVCRSQDTLPANRANLLFINNKNNTFSEQAQQY
                     GINDKGYASQGYFFDFDADNDLDLFICNHPYTWDNKIEKQAIQNATEDPFATDRLYRN
                     DGNKFTDVTKSAGVHSFAFSLSAAIADYNSDGLPDIYVCNDYTMNDFLYINNGNGTFT
                     DKVFDYFKHTSFYSMGSDMGDINNDGLTDLITLDMLPEDNRRQHLNMMRVDYDKYMAG
                     VKWGFGYAYMHNNLQLNQGNNRFAEIGQLAGIEATDWSWTPLLQDFDNDGYLDIYITN
                     GYKRDFTNADFIAYENEQARQQSSRPVSILDLLNKMPSIKISNYLYINNGKESGGLTF
                     KNATAQNQMNIPSFSQGAACADLDNDGDMDVLVNNINDQAFVFENTSNTEPENHFVNI
                     VLKGTGKNFNAIGATVKIANASGNQIANNMPMRGFLSTSQSLIHFGLGKNSEAITATI
                     KWPDGKMQQVTDLKIDKTNTINYNPDLVAANEKPETTKKLFSDETKKTKLDFSHIENE
                     YIDFKREPLLPHKYSENGPGVTVADINGDGMEDVFIGNSKIANGKFFIQQKDGTFASS
                     TSLPIVKSKTDDMGCMLFDTDNDGDNDLYIVSGGSEYDDSTAYNDHLFLNDGKGHFTE
                     NKSSLPIFNSSGSCIVAGDYDADGDLDLFRGGRITPGIFPLAPRSFLLRNDNGKFTDV
                     TPTLAPGLVKPGLVCAALFTDFDNSGTLDLIVTGEWMPLMIFKNEKGKFTNQTELAGL
                     NETNGWWNSIAAADFNNDGLTDYIIGNYGLNTRLRADKQQPASVTYKDFDDNGSLDAI
                     FCSPYPDGKSYPLVGRDEFIDQMRIFKKRLLRYDDYSGKTLSEIFTPDEMKGAQKVYA
                     YNFSSSVLINKGNGKFEINSLPVQAQFAPVYGVITNDFDNDGNMDALLTGNFYGAEVN
                     LARNDAFDGLFLKGDGKGAFTPVSHLQSGFMADKNSKGAAAVFINAQQKYKYIICNNN
                     DKAQICQTNSDNNTSVIIYKAKPTDCKLTYIDASGKKHLQELYFGSGYLSSSSRTLAI
                     PGNAKSIEVIDYSGRKTGIK"
BASE COUNT         1249 a          658 c          759 g         1189 t
ORIGIN      
        1 tccctttatg atgaaaaaaa taatttactt attaatgttc agttttattt attgttgttc
       61 tacaaaaaac gatgatggaa aaaccgaaaa tcttagcgca acaaagccat tattcaattt
      121 gctggctgtc gataaaaccg gtatcgattt taataacacg ttgaatgaaa ccgcaacaca
      181 aaatatttat gcttatcagt atttttataa tggtgcgggc gttgctgcag gtgacataaa
      241 caacgatggt ctggtagatg ttttttttgc cgccaatcag ggatcttgca aattatacat
      301 caataagggc gatttgaaat ttcaagacat tactctacaa tcgaaaattg ccactacagg
      361 ctggtgcaca ggcgttgcaa tggctgatgt aaatggcgat ggctggctcg atatttatgt
      421 atgccgttcg caagatacat tgcctgccaa tcgcgcaaat ttacttttca tcaataataa
      481 gaataacacc tttagtgagc aggcgcagca gtatggaatt aacgataaag gttatgcaag
      541 tcagggatat ttttttgatt ttgatgctga taatgatctc gatcttttta tttgtaatca
      601 tccttatacc tgggataata aaattgagaa gcaagccata caaaatgcca ccgaagatcc
      661 ttttgccacc gacagattgt atcgtaacga tggaaataaa tttaccgatg ttacaaaaag
      721 tgcaggagtg cactctttcg catttagttt aagtgccgct attgcagatt ataatagtga
      781 cggccttccc gatatttatg tttgcaacga ttacactatg aacgactttc tatatattaa
      841 taatggcaat ggaacattta ccgataaagt tttcgattat tttaaacaca cttcgtttta
      901 ctcaatgggc agcgatatgg gcgatatcaa caacgatgga ttaaccgatc tcatcaccct
      961 cgatatgttg cccgaagaca atcgcaggca acatctgaat atgatgcgtg ttgattacga
     1021 taaatatatg gccggtgtaa aatggggttt tggatatgca tacatgcaca acaatctaca
     1081 gttaaatcaa ggtaataatc gctttgcaga aataggtcaa cttgccggca ttgaggctac
     1141 cgactggagc tggacgccac tattacagga ttttgataac gatggatacc ttgatatata
     1201 tattaccaat ggatacaaac gcgactttac aaatgcagat tttatagctt atgaaaatga
     1261 gcaggcgcgt cagcagtctt ccaggccggt aagtatttta gatttgctta ataaaatgcc
     1321 atcgataaaa ataagcaact atttatatat taataatggc aaagaatcgg gaggattaac
     1381 atttaaaaat gctactgcac aaaaccagat gaacatccca agtttttcgc aaggtgctgc
     1441 ctgtgccgat ttggataacg atggcgatat ggatgtgttg gtaaataata taaatgatca
     1501 ggcatttgtt ttcgagaaca cttcgaatac cgagcccgaa aatcacttcg taaatattgt
     1561 attgaaaggc acgggaaaaa atttcaatgc cataggtgca acagtaaaaa tagcaaatgc
     1621 atccggcaat cagatagcca ataacatgcc catgcgcggt ttcttgtcaa catcgcaatc
     1681 gcttatacat tttggtttag gaaaaaattc ggaagcaata acagcaacaa taaaatggcc
     1741 cgatggtaaa atgcaacagg ttactgattt gaaaattgac aagaccaata ccatcaatta
     1801 taatccggat ttagttgctg ccaacgaaaa accagagacc acaaaaaaac tttttagcga
     1861 tgaaacgaaa aagacaaaac tcgatttttc tcatatcgaa aatgaatata tcgattttaa
     1921 acgcgagcca ttattgccgc ataaatattc ggagaatgga cccggtgtta cagtagccga
     1981 tataaatggc gatggcatgg aagacgtttt tattggcaat tctaaaattg ctaacggcaa
     2041 attttttatt caacaaaagg atggaacatt cgcttcgtca acatcgctcc ccattgtaaa
     2101 atcaaaaacc gatgatatgg gatgcatgct attcgatacc gataatgatg gcgacaatga
     2161 tttatacatt gtaagcggtg gcagcgaata cgatgattca acagcataca acgatcattt
     2221 atttttgaat gatggtaagg gacattttac cgaaaataaa agttccttgc ccatttttaa
     2281 cagcagtggt tcatgcatag tagccggtga ttatgatgcc gatggcgacc tggatttgtt
     2341 tcgcggtgga agaattacgc ccggaatatt tcctttagca ccgcgtagtt ttttactccg
     2401 aaatgataat ggaaagttta ccgatgtaac acccacatta gcacccgggt tagttaagcc
     2461 gggtttggtt tgcgctgcct tatttaccga ttttgataat agcggcactt tagatttaat
     2521 agttaccgga gagtggatgc cattaatgat ttttaaaaat gaaaaaggga aatttactaa
     2581 tcaaaccgaa ttagccggat tgaatgaaac caacggatgg tggaatagca ttgccgctgc
     2641 cgattttaat aatgatggat taacagatta cattattggc aactatgggt tgaatacacg
     2701 tttacgagcc gataaacagc aacccgccag tgttacttat aaagattttg atgacaatgg
     2761 cagtcttgat gcaatatttt gtagccccta tcccgatgga aaaagctatc ctttggttgg
     2821 ccgagatgag tttattgatc aaatgcgaat ttttaaaaag cgtttattgc gttacgatga
     2881 ttattcgggc aaaacactca gcgaaatttt tactcccgat gaaatgaaag gtgcacaaaa
     2941 agtttatgcc tataattttt cttcttcggt tttgattaat aaaggaaatg gaaagttcga
     3001 aataaatagt cttcctgttc aggcacaatt tgcccctgta tatggagtaa tcaccaatga
     3061 ttttgataat gacggcaaca tggatgcact gttgacaggt aatttttatg gcgctgaagt
     3121 taatttggcg cgcaatgatg ccttcgatgg tttattttta aaaggagatg gtaagggtgc
     3181 ttttactcct gtaagccatt tgcaaagtgg ctttatggca gataagaatt cgaaaggcgc
     3241 tgcagcagtt tttataaacg cacaacaaaa atataaatac atcatttgca ataacaatga
     3301 taaagctcaa atctgccaaa caaattccga taataataca agtgtgatta tatacaaagc
     3361 taaacctact gattgcaagt taacatatat tgatgcttcg ggcaaaaagc atctgcaaga
     3421 gttgtatttt ggttccggct atttgtcatc ttcatcgcgg accttggcaa tacctggcaa
     3481 tgcaaaatct attgaagtta ttgattacag cggtcgaaag acaggtatta agtgataaat
     3541 gattcaggaa ttttcataaa tggagtagat gttacttgtt ttctaactat agccatatac
     3601 gttttgtttt ttttcttgct ctgaatcctt tggcagcata ttaagttcaa aaaagatgat
     3661 ttatttctct actcatttaa agctattgat tcttttatat taagcagatt ctataacccg
     3721 atgtttttta tagatcggaa tttagagtcg tgttattttt ttttaaaaaa ataattgaat
     3781 ttgtgtaagc aagagttttt actattagcg accatttgtt tgtattttca atttttttac
     3841 gggatatttt ttttt
//
LOCUS       DAORQA010000171         3855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5750_length_3855_cov_7.948421, whole genome shotgun sequence.
ACCESSION   DAORQA010000171 DAORQA010000000
VERSION     DAORQA010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5750_length_3855_cov_7.948421"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /gene="paaC"
                     /locus_tag="PK736_07605"
     CDS             <1..674
                     /gene="paaC"
                     /locus_tag="PK736_07605"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:TIGR02158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HRC33294.1"
                     /translation="HGPILEEDIAMQNIALDLVGQSRIILSYAAQIEGKGKTEDDLAY
                     LRDAFSYRNLLMAEQPNTDFAHTMVRQFFMSAYQYFLYTHLHNSADETIKGFAQKSLK
                     ETIYHLRHSTDWILRLGDGTDESNMRTQTAVDELQQFIDDLFDADEVDAILLQQKIGA
                     DMEQVKKAWHEKVTEVFALAKIKWEPANGGTRKGSRRGIHTEYLGYILAEMQWLQRVY
                     PGNQW"
     gene            793..1302
                     /gene="paaJ"
                     /locus_tag="PK736_07610"
     CDS             793..1302
                     /gene="paaJ"
                     /locus_tag="PK736_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HRC33295.1"
                     /translation="MNNISHTHTNESIMQLLCTIPDPEVPAINIVELGVVRGVEIQND
                     TVQVTITPTYSGCPAMKAIEDEIVKTLLANNITQYKIKTVLLPPWTTDWLTDEAKQKL
                     QDYGIAPPQQTTNQHLTAMLSGKKIAVTCPYCKSGDTKLSSAFGSTACKALHYCNACQ
                     QPFEEFKCH"
     gene            1314..1745
                     /gene="ybeY"
                     /locus_tag="PK736_07615"
     CDS             1314..1745
                     /gene="ybeY"
                     /locus_tag="PK736_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRC33296.1"
                     /translation="MENNDIDITYQIKNVRIKDKRKTISWLLESAANEGFKAGFIYII
                     ICDEKYLMKMNKKYLQHNTITDVITFPYDMPGKNKIAGEIYISYPQVKYNAATYNVTV
                     SNELHRVMIHGLLHLCGYGDKTEKEKKIMTKMEDFYLERIW"
     gene            2204..2731
                     /locus_tag="PK736_07620"
     CDS             2204..2731
                     /locus_tag="PK736_07620"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HRC33297.1"
                     /translation="MAVASPFNLQKWIDDNRHLLKPPVGNKQVYIDNTDFIVMIVGGP
                     NSRKDYHFEDGEELFYQIEGDITVKTIQDGKNVDVHIRQGEMFLLPPHIPHSPQRGPN
                     TIGLVIERYRSEKEMDSCQWYCEKCATKLYEEFFPLTDIVNQLKTVMEKFYTNKDLCT
                     CKNCGYTMEEPPKLS"
     gene            2761..3771
                     /locus_tag="PK736_07625"
     CDS             2761..3771
                     /locus_tag="PK736_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRC33298.1"
                     /translation="MLTIDIHTHIIPENIPAYAQKFGYGGFIQLEHHKPCCARMMIGD
                     KFFREIQDNCWSAETRMKECDNHRVNVQVLSTIPVMFSYWAQPKHCLEVSQFLNDHIA
                     DIVQRYPQRFIGLGTVPLQDPQLTIEELHRCMQIGLRGVQIGSHVNDWNLNAPELFPF
                     FEEAAKLNASIFVHPWEMMGQQDMQRYWLPWLVGMPAETSRAICSMIFGGVFERLPNI
                     KVAFAHGGGSFPSTIGRIEHGYNCRPDLVAIDNPHNPRKYLDNIYFDSLVHDKLMLQY
                     LLNFTKPNRIALGSDYPFPLGEDIPGMLIKSMWLEDDVKQQLLNGTALEWLGMDRSYF
                     EV"
BASE COUNT         1278 a          758 c          765 g         1054 t
ORIGIN      
        1 gtcacggacc aattttggaa gaagatattg ccatgcaaaa tattgcgctc gaccttgttg
       61 gacagtcgcg cattatactt tcgtatgccg cacaaattga gggcaaagga aaaaccgaag
      121 acgacctcgc ctatttgcgt gatgcattca gctatcgaaa tttactgatg gccgaacaac
      181 ccaataccga ttttgcacat actatggtgc gccaattttt tatgagtgcg taccaatatt
      241 ttttgtacac acatttacac aacagtgccg atgaaaccat aaaaggcttt gcacaaaaat
      301 cgttgaaaga aactatttat catctgcgcc acagcaccga ttggatacta cgcttaggtg
      361 atggcaccga tgaaagtaac atgcgcacac aaactgcagt ggatgaactg caacaattta
      421 ttgatgacct ttttgatgcc gatgaagtag atgcaatatt gttgcaacaa aaaataggtg
      481 ccgatatgga acaggtaaaa aaggcatggc acgaaaaagt gacggaggta tttgcacttg
      541 caaaaataaa atgggagccc gcaaacggtg gcacacgcaa gggttcgcgc agaggcatac
      601 atacagaata cttaggatac atacttgccg aaatgcaatg gctgcagcgt gtatatccgg
      661 gtaaccaatg gtagggtaat gatgagaaac actttttgca acgaatgcac tcgaatgaat
      721 tcattgcatt attcggaatt tattcgcgca ttcgcggcaa cctgcatttt ttgattagac
      781 acaaaaattg aaatgaataa tatatcacac acacacacca acgaaagcat aatgcaattg
      841 ctatgcacca tacccgaccc cgaagtgccg gcaataaata ttgttgaatt aggtgtggtg
      901 cgtggtgtgg aaattcaaaa tgatacggtt caagttacca ttacgccaac atacagtggc
      961 tgcccggcca tgaaagccat agaagacgaa attgtaaaaa cattgcttgc caataatatt
     1021 acacagtata aaataaaaac ggttttatta ccaccatgga caacagactg gcttaccgat
     1081 gaagcaaaac aaaagttgca agactatggc attgcgccac cacaacaaac caccaaccaa
     1141 catttgactg ccatgttaag cggaaaaaaa attgctgtta catgtcccta ttgcaaatcg
     1201 ggcgatacaa aacttagcag tgcattcggc agcacagcat gcaaggcgtt acattattgc
     1261 aatgcctgtc agcagccctt cgaagagttt aagtgccatt aatttttttt gcaatggaga
     1321 ataacgatat tgatatcacc tatcaaataa aaaatgttcg cattaaggac aaaagaaaaa
     1381 caatttcgtg gctactcgaa agtgctgcca acgaagggtt taaagccggc tttatttata
     1441 ttattatttg cgatgaaaag tatttgatga aaatgaacaa aaaatatttg cagcataata
     1501 ccattaccga tgtaattact tttccttatg atatgcccgg caaaaataaa atagcgggcg
     1561 aaatttatat cagctaccca caagtaaaat ataatgccgc tacgtataac gttacagtta
     1621 gcaacgaact gcaccgcgtt atgatacatg gcttactcca cctctgcggc tatggcgaca
     1681 aaaccgaaaa agaaaaaaag ataatgacga agatggagga tttttatttg gagaggattt
     1741 ggtagtgaga tttgtagtat tgagtattga gacttggaga ttgggaattc ttgtcattgg
     1801 tcagtagtca ttggtatatc agttacttgt tattgggtta tttattattg aggattttta
     1861 ttcgtcagca gtcaataaca tcattgcgag cctcatttcc taccgtcatt gcgattccga
     1921 ttactatcgg aagaagcaat ctcaggatca tgaggtcttt tctaaaaagg gattgcttca
     1981 gtagttcgag agtactcact actcctcatt cgcaatgacg gaaggaaaat ttgtctccac
     2041 ataataaatg tttgaaaaaa ataaccaaca acccaataac caagtaacaa agcaaccaat
     2101 aacgcaataa ccaataacac agtaaccaat acccaacaac aaaaaacttg catttcaaat
     2161 ctagagtttc tttatttacc ataaacttaa aaaccaaaac attatggcag tagcatcacc
     2221 tttcaattta caaaagtgga tagatgacaa tcgtcattta ctaaaaccac ctgtgggcaa
     2281 taagcaggta tatatcgaca atacagattt tatagtgatg atagtgggcg gccctaactc
     2341 gcgcaaagat tatcacttcg aagacggtga ggaattattt tatcagatag aaggcgatat
     2401 aacagtaaag accattcagg atggaaaaaa tgtagatgtg catattcgcc aaggagaaat
     2461 gtttttgttg ccgccccata tacctcactc accgcagcgc ggaccaaata ctattggcct
     2521 tgtaatagaa cgctaccgca gcgaaaaaga aatggatagc tgccaatggt attgcgaaaa
     2581 gtgcgcaaca aaattgtacg aagaattttt tcccctcacc gatattgtaa atcaattgaa
     2641 aactgtaatg gaaaaatttt acaccaacaa agatttatgt acttgcaaaa attgtggata
     2701 tacgatggag gaaccaccaa aactttcata actatttttt tcaaataaat atattctcca
     2761 atgctcacca tcgatatcca tacacacata attcccgaaa acattccggc ttatgcacaa
     2821 aaatttggct atggtggttt tatacaattg gaacatcaca aaccttgttg tgcgcggatg
     2881 atgattggcg ataagttttt tcgcgaaata caagacaact gttggagtgc cgaaacacgt
     2941 atgaaagaat gcgataacca ccgtgtgaat gttcaggtac tttctaccat tcccgtaatg
     3001 tttagttact gggcacaacc gaagcattgc ttagaagtat cgcaattttt gaacgaccac
     3061 attgccgata ttgtgcaacg gtatccgcaa cgttttattg gtttaggtac tgtgccattg
     3121 caagatccac aacttactat agaagaactt caccgctgca tgcaaatagg attgcgcggt
     3181 gtacaaattg gttcgcatgt taacgattgg aatttgaatg caccggagtt gtttccgttt
     3241 tttgaagaag ccgcaaaatt gaatgcatct atttttgtac atccttggga aatgatgggc
     3301 caacaagata tgcaacgcta ttggttacct tggcttgttg gtatgcctgc cgaaacttcg
     3361 cgcgcaattt gttccatgat ttttggtggt gtgttcgagc ggttgccaaa tattaaagtt
     3421 gcttttgcgc atggcggagg ttcgtttcca tctaccattg gtcgcataga acatggttat
     3481 aattgccgcc ccgatttggt ggcaattgat aacccccaca acccacgaaa atatttagac
     3541 aacatttatt tcgactcact tgtgcacgat aaattgatgt tgcaatattt attaaatttt
     3601 acaaaaccca accgaatagc actcggcagc gattatcctt ttccgcttgg cgaagatatt
     3661 cccggcatgc tcattaaaag tatgtggctc gaagatgatg taaaacaaca attgctaaac
     3721 ggcactgcat tggagtggct tggaatggat agaagttatt ttgaagtttg ataataaaca
     3781 cttttcaaat cggatcccat attcggaaat attagccaaa aaaaatcaag gattgtctgc
     3841 aacacttttg ctttc
//
LOCUS       DAORQA010000172         3847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5765_length_3847_cov_10.689346, whole genome shotgun sequence.
ACCESSION   DAORQA010000172 DAORQA010000000
VERSION     DAORQA010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5765_length_3847_cov_10.689346"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1336)
                     /locus_tag="PK736_07630"
     CDS             complement(143..1336)
                     /locus_tag="PK736_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33299.1"
                     /translation="MIQSVREKYNAEFNPEKYKEFLEALEKTYNKEVVFRVAETPIFV
                     DKILRQKLIDASEQIVDIICKPDFKEASQRAIPAQYNVKNENDHTHFICIDFAICKDE
                     KGELTPQLIELQGFASLYSWQWFISKMYKEYFYCPEGFSPYFSGLNDKTYLDLLRKVL
                     IGKHELENVVLLELRPHEQKTRIDFYIANAELGINIVCLSEVIQRENKLFYKIDDREV
                     EIKRVYNRLIFDDLEANKEFKYDVDLFRDIDVEWVSHPNWFYRISKYTMPMLHNEFVP
                     ETFFLNELKTIPTDLENYVLKPLFSFSGMGVIIDVTRENIEDVTDPENWILQRKVQYA
                     EALASPEGGVKCEIRMMYLWPDGDTRPTLAVNLARMSRGKMIGVRYNSNFNWVGGTVA
                     FLDEN"
     gene            complement(1333..2157)
                     /locus_tag="PK736_07635"
     CDS             complement(1333..2157)
                     /locus_tag="PK736_07635"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HRC33300.1"
                     /translation="MHQIKIAILDMYKGEPNQGMRNIHELLDSYAANKKIDVHYKVYD
                     VRGKHEVPDSSYDAYIGTGGPGDPTEKEGWEMKWVMMMNSILHHNANNESKKLVFLIC
                     HSYQVMCLHFDIGIVCPRKRESFGIFPVHKTHESNTNQFMSQLHDPFYAVDSRKYQVV
                     EDENFNNNKLNAKIMAMEKIRDHVPLQRAIMALQFTPEIFGTQFHPEADALGMIYYLE
                     GEEKKELVIKNYGIHRYHSMLNHLNDPDKIMLTQLIFLPTFLDDVLGHTLVQQSNI"
     gene            complement(2206..3318)
                     /locus_tag="PK736_07640"
     CDS             complement(2206..3318)
                     /locus_tag="PK736_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HRC33301.1"
                     /translation="MDFSNFTLGIEEEYMVIDPVTRELKSHDQKIVEAASKILPQDQV
                     KAEMHEAVVEVGTGICKNTNEARADLAKLRRTINNVATEFGLRIGASGTHPFSKWEAQ
                     LITDAPRYKEIVAEMQDTARSNLIFGLHVHVGMESRELAIHIANAMRYFLPHVYALST
                     NSPFWEGRNTGFKSFRTKVFDKFPRTGIPDYFESIEDHDNYVKLLVKTGCIDNAKKIW
                     WDLRVHPFFNTVEFRICDVPMLLDETIAIAAVFQALCAKLFKLKSQNLNFINYKRLLI
                     NENKWRASRYGIDGRMIDFGKQTEVNTRILIVELLEFIEDVVDHLGSRKEIDYIYKML
                     EGGTGADRQLAVYEKNKSLNDVVDFILEQTLVGTQA"
BASE COUNT         1147 a          745 c          645 g         1310 t
ORIGIN      
        1 ctttatatgc aagggtttga ggagactatc accaactatt tttgaccatt atatcatttt
       61 tgttcaaact tttttgtttg tattattatt cgaagcagcc tgaaaaaaaa ataatattaa
      121 acccgaaaga aaataaatga ttttaatttt cgtccaaaaa agcaaccgtg ccaccaaccc
      181 aattgaagtt gctattgtaa cgtacgccaa tcattttacc tcggctcatt cttgcaagat
      241 taacggctag ggtagggcgg gtatctccat cgggccacag gtacatcata cgtatttcgc
      301 atttcacacc accttcgggc gatgccaatg cttcggcata ttgaactttg cgttgcagta
      361 tccagttttc gggatcggtt acatcttcta tattttcgcg tgtaacatct ataattactc
      421 ccataccgga aaaactaaat agtggcttca gtacataatt ttctaaatcg gtaggaatgg
      481 tttttaattc gtttaagaaa aaagtttcgg gcacaaattc attgtgcaac attggcattg
      541 tgtatttact aatacgataa aaccagttag gatgcgatac ccattctaca tctatatcac
      601 gaaataaatc aacatcatat ttaaattctt tattggcttc gaggtcgtca aaaatcaagc
      661 gattatatac acgcttaatt tctacttcgc ggtcgtcaat tttgtagaat aatttatttt
      721 ctctttgaat aacttcgcta agacaaacta tattaattcc taattcggca tttgcaatgt
      781 aaaaatcgat gcgtgttttt tgctcatgtg gtcgtagttc aagtaagaca acattttcca
      841 attcgtgctt gccaattaaa actttgcgca ataaatcgag ataggtttta tcattcaatc
      901 cactgaagta tggcgaaaaa ccttcggggc aataaaagta ttccttatac attttggaaa
      961 tgaaccactg ccaactgtat aaacttgcaa atccctgcag ttctatcaac tgtggtgtga
     1021 gttcgccttt ttcatctttg caaatagcaa aatcgataca aataaaatgg gtgtgatcgt
     1081 tttcgttttt tacattgtat tgtgcaggta tagcccgttg tgatgcttct ttaaaatcgg
     1141 gcttgcaaat aatatcaaca atttgttcgc ttgcatctat caatttttga cgcaaaattt
     1201 tgtcaacaaa tatgggcgtt tcggcaacac gaaacactac ttctttgttg taggtttttt
     1261 ctaaagcctc taaaaattct ttatattttt ccggattaaa ttcggcatta tatttttcgc
     1321 gaacagactg tatcatatat tcgattgttg aactaaagtg tgaccaagaa catcgtcaag
     1381 gaaagttgga agaaatatta attgcgtaag cattatttta tcggggtcgt tcaagtgatt
     1441 gagcatactg tgataacgat gtattccata attttttatt accagttctt ttttctcttc
     1501 gccttcgagg taatatatca tacccaaagc atcggcctcg ggatgaaact gagtgccgaa
     1561 tatttcggga gtaaattgca gcgccataat tgcacgttgc agaggcacat gatcgcgaat
     1621 tttttccatg gccataattt tcgcgttcag tttattatta ttaaaatttt cgtcttccac
     1681 tacctgatac ttacgtgaat ccactgcata aaatggatcg tgcaattgtg acataaattg
     1741 atttgtattg ctctcatgtg ttttgtgtac aggaaatatt ccgaatgatt ctcgtttacg
     1801 tggacaaact attccaatat caaaatgtaa gcacataacc tgataggagt gacaaataag
     1861 aaaaactaat tttttacttt cgttattggc attgtgatgt aatatagaat tcatcatcat
     1921 tacccatttc atttcccacc cttctttttc ggtagggtca ccggggccgc ctgtaccaat
     1981 atatgcatcg tatgagctat cgggcacttc atgcttgcca cgcacatcat acactttgta
     2041 atgcacatca atttttttat ttgcggcata gctatccaat agctcgtgta tgttgcgcat
     2101 accctgattg ggttcgccct tgtacatatc gagtatggct atttttattt gatgcatatt
     2161 gtaaaaaatg aattaatatt ttgaattcaa cagtaaagca tgaaattacg cttgcgtacc
     2221 tacaagtgtt tgttctaaaa taaaatctac tacatcgttt aagcttttat ttttttcata
     2281 aacggccaat tgtctgtcgg cacctgtgcc accttccagc attttataaa tgtagtctat
     2341 ttcttttctg cttccaaggt ggtctaccac atcttctata aactccaaca attcgacaat
     2401 gagtatgcgc gtattcactt cagtttgttt tccaaaatca atcatacgcc cgtcaatacc
     2461 atagcgagaa gcgcgccatt tattttcatt aatcagtaaa cgtttgtaat taatgaagtt
     2521 taagttttga gatttcaatt taaataattt tgcacacaaa gcttgaaaaa cggctgcaat
     2581 agcaatggtt tcgtcaagta acatgggcac atcgcatatg cgaaattcaa cggtattgaa
     2641 aaaagggtgt acacgcaaat cccaccatat tttttttgcg ttatcaatac aacctgtttt
     2701 cacaagcaac tttacatagt tgtcgtggtc ttctatactt tcaaaataat cgggaattcc
     2761 tgtgcgggga aatttatcga atactttggt gcggaatgat ttgaatcccg tattacggcc
     2821 ttcccaaaat ggagagtttg tagacagtgc ataaacgtgt ggcaaaaaat aacgcatagc
     2881 atttgcaata tgaatggcca actcgcgcga ttccattcca acatgcacat gcaaaccaaa
     2941 aatgagattg cttcttgcag tatcttgcat ttcggcaaca atttctttat accgtggtgc
     3001 atcggtaatt aattgtgctt cccacttgct aaatggatga gtgccacttg caccaatgcg
     3061 taaaccaaat tctgtggcaa cgttatttat tgttctgcga agtttggcta agtctgcacg
     3121 ggcttcattg gtgttcttgc aaatgcctgt tcccacttcc acaaccgctt cgtgcatttc
     3181 tgctttcacc tggtcctgag gcaaaatttt tgatgctgcc tcaactattt tttgatcatg
     3241 cgattttagt tcccttgtta cagggtctat taccatgtat tcttcttcaa tacctaaagt
     3301 gaaattgctg aaatccataa atgattattt gtttttaaaa tatgtctcga ttcgtttctg
     3361 gcattaaaac caatttgcga tacaaatgcc gcgctaaaat acatatattt aaaataatgg
     3421 ttatcatatt tttacgcttt tattaactat tgaccaaaaa aaaactctta caaatattgc
     3481 agttgcaaaa tttgtaagag cctatttgtt ttaagcaagt tttgaatgcg aattaatcag
     3541 tgtttttcta attttaattt agtagaactc aaactgacca ataaatttat tttagaaatt
     3601 gttgactcaa aaccacaaac tccaagctaa aaaccagaaa ctgaaaattc cagaatagaa
     3661 actaaaaact ctaaactaaa aacttcaaac taaattaaac tttgcctgca tttaactttg
     3721 cctgcaactt gctaagttca tcccacttgc catcgctggc aagtttactt tgctctggcc
     3781 atgaagcagg attatgcatt ttgaatttgc tgcctgcacc atcaagaata tccttaatgt
     3841 gttttgc
//
LOCUS       DAORQA010000173         3827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5811_length_3827_cov_8.480647, whole genome shotgun sequence.
ACCESSION   DAORQA010000173 DAORQA010000000
VERSION     DAORQA010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5811_length_3827_cov_8.480647"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PK736_07645"
     CDS             <1..542
                     /locus_tag="PK736_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017104178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRC33302.1"
                     /translation="DNIICGIFLIDFIIRFKRAENKLHFMKWGWIDLISSIPMLDFFR
                     AGRALRLIRLLRILRAFRSTKHLVQHLFKSRTQGTFSAALIIAMLMVIFSSIAILQVE
                     TDPNSNIRNAEDAIWWAFVTITTVGYGDKFPITTEGRIIAALLMTVGVGLFGTFTGFV
                     ATWFIAHPQTNILNDEEAE"
     gene            1536..2969
                     /locus_tag="PK736_07650"
     CDS             1536..2969
                     /locus_tag="PK736_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33303.1"
                     /translation="MKKFLLFFALVLVSFQGYAQVIYGVNNYTQYHIGSLPIVISVPH
                     GGLVAPVIIPDRTCNSPTTVWDNYTIELGRQIDTALYNLTGCHPHLIICNMRRTKVDC
                     NRNIADGACGNSQAETVWTEFQHFIDTAEILAQSQFAGKAFYIDLHAHGHAIKQLELG
                     YGISGAGLTNTDNVLNTAPYIAASSIENLVATNVNGYTHAQLLRGTYGLGTLFVDAGF
                     PSVPSMQTPNPGGNPYFSGGYNTYNHTCILQGNTVNGVQIETPSTVWSSYLNRKKFAD
                     STAAVLASYLMIHQNINLITNCGIPFTPLPISLIDFSGKAAEEQNILWWTTANEINND
                     YFTLMKSSDGINFESIAKIKGAGNSTHILNYSFDDRHLTSAIHYYKLQQTDYNGQSTF
                     SKVIRINNSADEVFVSPNPVKDFIQLSFSPLENNFEVELLNTFGETMLKTNNQIRLNV
                     SHFPAGIYLLKITDCHNHVQTKIIVKQ"
     gene            3263..>3827
                     /locus_tag="PK736_07655"
     CDS             3263..>3827
                     /locus_tag="PK736_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC33304.1"
                     /translation="MKLLVTTDFSANSKGAIRFAQTLAKQSNTIEVTFYHSVHFMKPT
                     SWNDAFYNSYKREEIERLTTELKTFVNSTIEQDKDKFANIKFVIDSAISTEKQIIIYA
                     EKNKMDFICIATQGAGVLRKIMGTHTSHIVNNSRIPVLVIPSHYRIKPLKKLTYLSDF
                     ENVKKELDKVTKFSESAQCRLDVLHYSY"
BASE COUNT         1338 a          658 c          658 g         1173 t
ORIGIN      
        1 ttgataatat tatttgtggt atttttttga tcgattttat aataagattt aaaagggcag
       61 aaaataaact gcactttatg aaatgggggt ggatagattt aatttcaagc atcccaatgt
      121 tggacttttt tagggcaggc agagcattac gtttgatacg attattaaga atattaagag
      181 catttcgttc aaccaaacat ttagtacaac atttgtttaa aagtagaacc caagggacat
      241 tttctgccgc actaattatc gcaatgttaa tggttatctt ttcttcaata gcaattctgc
      301 aggtagaaac agaccctaat agtaatatta gaaatgccga agatgcaatt tggtgggcat
      361 ttgtaacaat taccacagtt ggatatggcg ataaatttcc aatcacaaca gaggggagaa
      421 taattgccgc acttctgatg acggtaggag ttggcctttt tggaacattt actggatttg
      481 tagcaacttg gtttatagca catcctcaaa ctaatatttt gaatgatgag gaagcagagt
      541 agttatactg gatgtctgtt tattgcaata tttttgtttg taggtgacct tttttttatg
      601 ttttcaaaaa agaattatca ggaattataa cagtttccga acccacaatt attcaagata
      661 cccttttaaa tttttcatta cctaagaaag atatccaaaa aacaaaaaag agaacaaaaa
      721 acaatactta atctattaca aatactggca atactaaaac aattgcaaaa agaaaggtaa
      781 aagttagaaa ggcaaagcaa agtaggaatg gtagttactc aaatacaaga acctaccaaa
      841 gccaggaatc aagtgtatat ttaagaaaat gttcaggaag aacacaaaaa ggtttaagat
      901 gcagaaactt cacaagaagc agtaatgggt tatgttggag acatggtggt taataatgat
      961 atagcaaata gtggcttacg caagtgtggc attattgttt gaaataatat ttatttttct
     1021 ttatcatttg ttgataaaca aaatttagtg cttaaattaa acgcctgact taaagcccca
     1081 aatcgtcaga ccttgaagaa tccacctttg aaaatattgc caaccaaaac aattacataa
     1141 cgacaaccat atacgataca ttccttctgc cttcagctat gtaatgcaca tttatttttt
     1201 ttgaaggcat gtctacagtg tcgttattat tttcgctttt accacacgat gttgacttgc
     1261 aaatttggtc tcgttcattt tttttgcagt ctgcatttta taattaattc ttgaaagcaa
     1321 tgcttcaaat aaaaaaggtc ataaaaactt atataaaatg cagatagcga aaccgtaatg
     1381 caaacctatt gcaattgcag gctcatgaac tcggatgccc gttcttttta aaaactgcgt
     1441 aatccaaaat tttgcgcttt ctttgcaatt gtaactatgt gttcaatcat ttatatttta
     1501 cagagtgaac tatgtttatt aataagatat ttctaatgaa aaaattctta cttttttttg
     1561 ccttagtcct tgtaagtttt caaggatatg cacaagtaat ttatggtgta aataattata
     1621 cccaatatca tattggtagc ttacctattg ttatttcggt tcctcatgga gggcttgttg
     1681 ctcctgtaat cattcccgac cgaacctgca acagtccaac tactgtgtgg gataattaca
     1741 ctattgagct tggccggcaa atagatacag cactgtataa cttaaccggt tgccatcctc
     1801 atttaataat ctgcaatatg aggcgcacta aagtagattg taatcgcaac atagctgatg
     1861 gtgcatgtgg caatagccag gcagaaactg tatggaccga atttcaacat tttatcgata
     1921 ctgcggagat tttggcgcag agccagtttg cgggcaaagc attttatatt gatttgcatg
     1981 cccatggtca cgctattaaa caattagaat tgggatatgg catttcgggt gccgggctta
     2041 ccaatacgga caatgttcta aatacagcgc catacattgc tgccagttca atcgaaaatc
     2101 ttgttgctac caatgttaat ggatatacgc atgcacaact attaagaggc acttatggat
     2161 taggcacttt gtttgtggat gccggctttc cttctgtacc aagtatgcaa acacccaatc
     2221 cgggaggtaa cccttatttt tcgggtggat acaatacata taaccatact tgtatactac
     2281 aaggcaatac ggtgaatggc gtgcaaatag aaacgccttc aacagtgtgg agcagttatt
     2341 tgaatcggaa aaagtttgct gattctactg cagccgtgct tgcaagctat ttaatgattc
     2401 atcaaaacat aaatttaata acaaattgcg gtataccttt cacaccacta ccaatttcgt
     2461 taatcgattt ttctggaaag gccgctgaag agcaaaatat tttatggtgg acaacagcaa
     2521 acgaaatcaa caacgattat tttacgctaa tgaaaagcag cgatggaata aattttgaat
     2581 ccattgcaaa aataaaagga gcaggcaaca gcacacacat attaaattat tcttttgacg
     2641 accgacatct tacttcggca atacattatt ataaactgca gcaaacagat tacaacggac
     2701 aaagcacatt ctcaaaagtt attcgaataa ataattctgc ggatgaagtt tttgtttctc
     2761 caaacccggt aaaagatttt attcaacttt ccttttcacc attggaaaat aattttgaag
     2821 tggaattgct aaataccttt ggtgaaacaa tgctgaaaac caacaaccaa atcaggttga
     2881 atgtttcgca ctttcctgca ggaatttatt tgttgaaaat aacagactgt cacaaccacg
     2941 tgcaaaccaa aataattgta aagcaatagc gaaacaacta ttattaaaag ctaatagcta
     3001 aaaccgactg taacttcttc gcaagtcatg tttatttctt aacggtcaca tttctctaaa
     3061 gaaatttcaa gtatcagtgt cgaaaaaatg agacctcaat ccaaactttt caacaccaag
     3121 catcagacat tttaaatcaa aaaagccctg caagttatta catatagggg gattgaaaat
     3181 tcatttgtat gggaacaatt tgtgatttta aaaaaaaata aaattaactt tgcaaaaagt
     3241 attaataata aaaaaaacat atatgaaact attggtaact accgattttt cggcaaattc
     3301 gaaaggggca atcaggtttg cacaaacgct ggcaaagcaa tccaatacta tagaagtaac
     3361 attctaccac tctgttcatt ttatgaaacc aacaagttgg aacgatgctt tttataacag
     3421 ctataagcgt gaagaaattg aaagactcac caccgaactg aaaacatttg taaattcaac
     3481 tatagagcaa gataaagata aatttgccaa cattaagttt gtgattgatt ctgctatttc
     3541 gaccgaaaag caaattatta tatatgccga aaaaaacaaa atggatttta tctgtattgc
     3601 aacacagggt gccggtgtat taagaaaaat aatgggcaca catacctctc atattgtgaa
     3661 taattccaga attcccgttt tggtaatccc cagtcactat cgaataaagc cattaaagaa
     3721 attaacttat ctgtcagact tcgaaaatgt gaaaaaagaa ttagacaagg tcaccaaatt
     3781 ttcagaatcg gcacaatgcc gtttagatgt attgcattat tcatata
//
LOCUS       DAORQA010000174         3822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5820_length_3822_cov_12.125830, whole genome shotgun sequence.
ACCESSION   DAORQA010000174 DAORQA010000000
VERSION     DAORQA010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5820_length_3822_cov_12.125830"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PK736_07660"
     CDS             <1..296
                     /locus_tag="PK736_07660"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33305.1"
                     /translation="FADLIEMEYRNRICDNANENYLFNLAEYETLHQKLLETTLHQKM
                     EIPGMLKPRADMIAIASIITTFVFRKINGRQMFMSRNSLREGVLQHLAEGKKL"
     gene            340..1503
                     /locus_tag="PK736_07665"
     CDS             340..1503
                     /locus_tag="PK736_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRC33306.1"
                     /translation="MAKVLVIDDEKSIRNSLREILEYESYIVDDAPDGIEGLKMAEKS
                     TYDIILCDIKMPKMDGMEVLDKLREKNIDAPVVMISGHGNIETAVEAIKKGAFDYISK
                     PLDLNRLLITIRNAMDKSTLITETKILKKKIFKTTAMIGSSESIQKVKDIIEKVAPTD
                     ARVLVTGGNGTGKELVARWIHEKSNRAALPLVEVNCAAIPSELIESELFGHEKGSFTS
                     AVKQRIGKFEQANGGTLFLDEIGDMSLSAQAKVLRALQEGKVTRVGGEKDFSVNVRVI
                     AATNKDLKAEIERNNFREDLYHRLSVILIKVPSLNERKEDIPLLVEHFIKDMADDGGM
                     PLKKFSDGAIKELQKMNWTGNIRELRNVVERLLILGDKIITDKDVQMFAGIGK"
     gene            1516..2034
                     /locus_tag="PK736_07670"
     CDS             1516..2034
                     /locus_tag="PK736_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRC33307.1"
                     /translation="MTITKPLQKDCSEYAWSYINKVEQTNLLTALEVITKSTTDLFSH
                     FHGEQLEYTYAPGKWNVKQILNHITDSERIFAYRILRFARKDSTELAGYDEDLFAKND
                     NTQNRTMDSLIDEYIHVRKSTILLLENFDDEILEIAGTANKNAFTIRTWAWMIAGHNQ
                     HHNEVVRLKYKK"
     gene            2210..>3822
                     /gene="yidC"
                     /locus_tag="PK736_07675"
     CDS             2210..>3822
                     /gene="yidC"
                     /locus_tag="PK736_07675"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRC33308.1"
                     /translation="MDKNTVTGLLLMGMVLIGYTFFMAPGKEEIAAQKQTQDSLILVQ
                     KQRSADSVEAFKTNNTIASQATDIAAVLQNDSAKNVAMASTYGAFVNAAPAAEKLVYI
                     ENEKVKITLSSVGGKVKQVQLKNLKTFDGAPLQFYDDDSVGMGLSFFSENKSINTQQL
                     HFEPQGTSFAVNGKNKKELIMRCDAGSGRHLDYIYTLEGNSYNLKFDIQLQGLNNVIA
                     ANSSYLELDWHASILKQERTVNGERQYSSVYYSFADNEVNNLSETSDEKESLNEKVKW
                     VGFKQHFFSSVLIADKMFDPAVIEARTDLGSDTIVRYMSANLRIPYDHKADEKFGMSF
                     YFGPNHFQTLKSYNLGMEKLIPLGWGILGWINRFIVIPVFNWLKNFGISFGLVILILT
                     IIIKLILLPLNYRAYLSSAKMRVLQPEVTELQTKFKEEPLKMQQEMMGLYRKAGVSPF
                     GGCWPMLMQMPILFAMLRFFPNSIELRQESFLWAKDLSTYDSILNIGFHIPGYGDHVS
                     LWTILMTISTLLFTYFNSQQLNTAANPAM"
BASE COUNT         1311 a          669 c          784 g         1058 t
ORIGIN      
        1 cctttgccga cctcatcgaa atggaataca gaaacagaat atgtgacaat gctaacgaaa
       61 actatttgtt caatttagct gaatatgaaa cactacacca gaaattactc gaaacaacgc
      121 tgcaccaaaa aatggaaata cccggtatgc taaagccccg tgccgatatg attgctattg
      181 catcaataat aactactttt gttttccgaa aaataaatgg caggcaaatg tttatgtctc
      241 gaaactcgct acgcgaaggg gttctacaac atttagcaga aggaaaaaaa ctttagtgat
      301 attttataga aaacaacaat tgaaacaaaa aaactaatta tggcaaaggt tcttgtgata
      361 gatgatgaaa aaagtattcg caactcgcta cgcgaaatat tggaatatga aagctacatt
      421 gtggacgatg cacccgatgg aattgaaggc ttgaaaatgg ctgagaaaag cacatatgat
      481 attattttgt gtgatataaa aatgcccaaa atggatggca tggaagtgct cgacaaattg
      541 cgcgaaaaaa atatcgatgc tcccgttgta atgatatcgg gtcacggcaa tattgaaaca
      601 gcagttgaag caattaaaaa aggtgcgttt gattatatct cgaaaccact cgaccttaat
      661 cgtttactta ttaccattcg caatgccatg gataagtcta cgcttattac tgaaacaaaa
      721 attttgaaaa agaagatttt taaaactact gctatgatcg gcagcagcga atcgatacaa
      781 aaggtaaagg atataataga aaaagttgca ccaaccgatg cacgggtatt ggtaacaggt
      841 ggcaatggca caggaaaaga actcgtggca aggtggatac atgaaaaaag taatcgtgct
      901 gcattgcctt tagtggaagt aaactgtgct gcaattccat cggagcttat cgagagtgaa
      961 ttatttggtc acgaaaaagg ttcgtttaca agtgcagtta aacagcgcat aggaaaattt
     1021 gaacaagcca atggcggcac tctttttctt gatgagatag gcgatatgag tttgagtgca
     1081 caagcaaaag tacttcgcgc attgcaagaa ggcaaagtaa cacgagtggg tggcgaaaaa
     1141 gatttttcgg ttaatgtgcg ggtaatagca gccaccaata aagatttgaa agctgagata
     1201 gaaagaaata attttcgcga agacttgtat catcgtttaa gtgtaatact tattaaagtg
     1261 ccatctttaa atgagcgaaa ggaagatatt ccacttttgg tagaacactt tattaaagat
     1321 atggccgatg atggtggaat gccattgaag aaattttcgg atggcgctat aaaagaattg
     1381 caaaaaatga actggactgg caatattcgc gaattgcgca atgtggtgga acgactgctt
     1441 attttaggag ataaaattat cacagataaa gatgttcaaa tgttcgcagg aattggcaaa
     1501 taaatagtaa taagaatgac aattacaaaa ccacttcaaa aagattgttc cgaatatgct
     1561 tggtcgtata ttaacaaagt ggaacaaaca aatttgctca cggcactcga ggtaataact
     1621 aaaagcacaa cggacctatt cagccatttt catggcgagc aactcgaata tacttatgca
     1681 cctggaaagt ggaatgtgaa acagatattg aaccacataa cagatagcga gcggattttt
     1741 gcttaccgta tattgcgctt tgcacgaaaa gacagtacag aacttgcagg gtatgacgaa
     1801 gacctatttg caaaaaacga caacacgcaa aatagaacaa tggatagttt aattgatgaa
     1861 tacattcatg tgcgaaaatc gaccatacta ttattggaaa actttgacga tgaaattctg
     1921 gaaattgcag gcacagccaa caaaaacgca tttaccattc gcacctgggc ctggatgatt
     1981 gcagggcaca accaacacca taatgaggtg gtgaggttaa agtataaaaa gtaacaaact
     2041 tacatttagt taattattaa gctatattca gcttacaacc ttttgctatg gccactttca
     2101 tgcaaagcct ttgctttttt acccttacaa tcaatgatga taacaataaa tgtaaaccct
     2161 ttcaatatcg aactttcgat ttatatttgc gctcccaaaa aataaatgaa tggataagaa
     2221 tacagttact ggtttgctgc tgatgggtat ggtgctaata ggctatacat tttttatggc
     2281 gcctggtaaa gaagagatag cagcacaaaa acaaacacaa gactccttaa tattagtgca
     2341 aaagcaaaga agtgctgata gtgttgaggc ctttaaaacc aacaatacta ttgcttcgca
     2401 agccactgat atagcagcag tcctgcaaaa cgactctgct aagaatgtag ctatggcatc
     2461 cacttatgga gcatttgtaa atgcagcacc cgcagccgaa aagttggttt acattgagaa
     2521 tgaaaaagta aaaatcacat tatcatctgt cggtggcaag gtgaagcaag tgcagttaaa
     2581 aaatctaaaa actttcgatg gtgctcctct acagttttat gatgacgact ctgttggcat
     2641 gggtcttagt ttttttagcg agaataaatc tataaacacg caacagttac atttcgaacc
     2701 acaaggaaca agttttgctg ttaatggtaa aaacaaaaag gagttgataa tgcgttgcga
     2761 tgccggcagt ggtcgccatc tcgattatat ctatacttta gaaggtaata gttacaactt
     2821 gaaattcgac atacaattgc agggacttaa taatgtaatt gcggcaaatt cttcttacct
     2881 cgaattagac tggcatgcat caatattaaa gcaggaacga actgtaaatg gtgaaaggca
     2941 atactcatcg gtatattata gctttgctga taatgaggta aataatttat cggagactag
     3001 cgatgaaaaa gaatcgctta acgaaaaagt gaaatgggta ggctttaagc aacatttctt
     3061 ttcatcggtt ttgattgccg ataagatgtt tgaccctgca gtaatagaag cccgcaccga
     3121 tttaggaagt gatactattg ttcgatacat gagtgccaat cttcgcatac catacgacca
     3181 taaagccgat gaaaaatttg gcatgtcatt ttattttggc cctaaccatt ttcaaacgct
     3241 aaagtcgtac aaccttggaa tggaaaagct tattccactg ggttggggaa ttttaggttg
     3301 gataaatcgc tttattgtta ttcctgtttt caactggctt aaaaacttcg gaataagttt
     3361 tggtttggta attcttattc tcaccattat tataaagctg atcctgctgc cattaaatta
     3421 tcgtgcatat ttatcgagcg ctaaaatgag ggtattgcag ccggaagtaa ccgaattgca
     3481 aaccaagttt aaagaagagc cattaaaaat gcagcaagaa atgatgggcc tttatcgaaa
     3541 agccggagta agtccttttg gtgggtgctg gcctatgctg atgcaaatgc caatactgtt
     3601 tgccatgttg cggttttttc ccaactcaat agaattgcgt caggaaagtt tcctttgggc
     3661 caaagattta tccacttatg actccattct caatatcggg tttcacattc ccggttatgg
     3721 tgaccacgtt agcttgtgga ccattttaat gacaatttca actttgttgt ttacctactt
     3781 caactcgcaa caattgaata ccgctgctaa cccggctatg aa
//
LOCUS       DAORQA010000175         3807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5847_length_3807_cov_11.419510, whole genome shotgun sequence.
ACCESSION   DAORQA010000175 DAORQA010000000
VERSION     DAORQA010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5847_length_3807_cov_11.419510"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PK736_07680"
     CDS             <1..708
                     /locus_tag="PK736_07680"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRC33309.1"
                     /translation="FKRKIQADLIPKLFIIILLGAWQGFLGWYMVKSGLVNNPHVSHL
                     RLAMHLVNAFITFGYIFWVALDLQYYKDTLTSKRPKTKATEVTIGLLIIVLLQIIYGA
                     FVAGLKAGYVYNTWPLMDGQIVADSVFKAFNYDGIFSLVNNLATVQFIHRTLALIIFF
                     GIVWFWFNRHHESFSLKAEQKQGVISCMLIVLLQVVLGICTLIFHMPMALAIIHQATA
                     FLLFGSFIFLWHRLRFA"
     gene            809..3202
                     /locus_tag="PK736_07685"
     CDS             809..3202
                     /locus_tag="PK736_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC33310.1"
                     /translation="MFPMLNDYLKRILLVFLLIPVFGTQANLFAQHQGQKIRGIIVDK
                     QTKQPLPGASVVMLDSVNFLGTVTNNLGQFRLAKVPLGRRIIKVSYVGYTESTYTVIV
                     TSGKEVVLEIALEEKVYNGNEIVVTAERQKDKAINQMASVSARSFTFEETSRYAGSLN
                     DVARMAANFAGVSGANDGRNDVIIRGNSPTGVLWRLNGIDIPNPNHFGSLGSTGGPIS
                     ILNNNHLAQSDFFTSAFPAEYGNALAGAFDLQLRPGNNEHHEFLGQVGFNGLEVGAEG
                     PVNSKTGASYLINYRYSTLGIFKSMGINFGTGAAVPQYQDINYHFNFPTKKAGRFSFF
                     GIAGKAYVELLDSEKDTTKNNLFDSEEPQNGYFGNSMGVAGVQHLYIFKNNMYLKTTM
                     AVSGISQDWKIDSLGADDYKPYPFYRNNSYSIRSTLNTQFQKKINTRNVFKAGIIVEQ
                     YNFQFADSVLDNDEFRIISKSKGGMQVYQAYVQWQHKFSDHSTLNAGVHSLLLGLNKT
                     YSIEPRLGYRFEFNPRHAISFGAALHTQMQPVYFYFIETRTGLNQFTQTNRNLSLTKS
                     AQAVVAYDFKFRKDFRIKLETYYQHLFDIPVEKRSTSYSLINEGADFALSTRDSLVNN
                     GTANNKGLEITIEKFYSHGYYFLFTSSLFQSLYKGSDKVTRNTAFNGQYIFNALAGKE
                     WTIKKDNVISINFRSTYAGGKRYTPIDLQRTIIEQETVLDPTRAYSAQYKNYFRTDVK
                     FSYRINGKKVTQEFSLDINNVFNIKNIWQQQYNPKSGKIVTEFQIGFFPIPLYRIYF"
     gene            3212..3676
                     /locus_tag="PK736_07690"
     CDS             3212..3676
                     /locus_tag="PK736_07690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC33311.1"
                     /translation="MNLSELIWRTKKFDELTLDQLYAILKLRMEVFIVEQNCFYLDLD
                     DVDKVCLHLYATDSADNIAAYARAIPQGAVYKSSSFGRVVVHPQWRGTGLGKLLTKNV
                     MEVIENNFGTTEIKISAQSHLEKFYVSFGFVKCGDEYMDAGILHIPMTILRF"
BASE COUNT         1113 a          748 c          766 g         1180 t
ORIGIN      
        1 tttaaaagaa aaattcaggc cgatttaatt cccaaactgt ttatcattat tttgctcggt
       61 gcctggcaag gctttttggg ttggtatatg gtgaaaagcg gtttggtaaa taacccacac
      121 gtaagtcatt tgcgattggc catgcatctt gttaatgcat ttataacttt tggatacata
      181 ttttgggtgg ccctcgattt acagtattat aaagacacgc tcacatccaa acgtccaaaa
      241 acaaaagcca ccgaagttac aattggcctg ctcattattg ttttgttgca aattatatat
      301 ggtgcttttg tggcgggtct taaagccggt tatgtatata atacctggcc gctaatggat
      361 ggacaaattg ttgccgactc tgttttcaaa gcatttaatt acgatggaat ttttagcctc
      421 gtaaataatc ttgctacggt gcagtttatt catcgcacat tggccttaat tatcttcttt
      481 ggtattgttt ggttttggtt caaccgccac catgaatcat tttcgttgaa agccgaacaa
      541 aagcaagggg tgatttcttg tatgctgatt gtattgctgc aagtggtttt aggaatttgt
      601 actttgattt tccatatgcc aatggctttg gcaataattc atcaggcaac ggcattttta
      661 ttgtttggct cattcatttt cctttggcat cgtcttcgat ttgcttaatt aaaatcaaca
      721 tgggctgcat tttttttatt tggactgaag agattacacc acaactcgac tttttggttt
      781 ttgcaataac tacctttgaa acgaatttat gtttcccatg ctaaacgatt atcttaagcg
      841 catactgttg gtttttctac tgattcctgt ttttggaact caagccaact tgtttgctca
      901 acatcaggga caaaaaatac gcggcataat tgtcgacaaa caaaccaagc aacctttacc
      961 cggagcaagc gttgtgatgc tcgattcagt taatttcctg ggaaccgtta ccaataacct
     1021 tgggcagttc cgtttagcaa aagtgccact tggacgaaga atcattaaag tgtcgtacgt
     1081 tggctatacc gagagtacgt atacagtaat tgttacttcg ggcaaagaag tagtactcga
     1141 aattgcactc gaagaaaaag tgtacaatgg aaacgaaatt gtagttacag ccgagcggca
     1201 aaaagataaa gccatcaatc aaatggcaag tgtaagtgca cggtcgttta cctttgaaga
     1261 aacatcgcgc tatgccggca gtctcaacga tgtagcccgc atggctgcta actttgccgg
     1321 agtaagtggt gccaacgatg gtcgcaacga tgttatcatt cgtggcaatt cgcccaccgg
     1381 tgtattgtgg cggctcaatg gtatcgatat tcccaatccc aatcattttg gatcgctggg
     1441 ttctaccggt gggcctataa gtatactcaa caataatcat ctggcacaaa gcgatttttt
     1501 tacttccgca tttcctgccg aatatggcaa tgcacttgca ggtgctttcg atttgcaact
     1561 tcggccgggc aacaacgaac atcatgagtt tttgggacag gtaggtttca atggtttgga
     1621 agttggtgcc gagggtccag ttaattctaa aacaggagct tcgtatttga tcaactacag
     1681 gtattctacg ttaggcattt tcaaatcgat gggaataaat ttcggcaccg gtgcagctgt
     1741 tcctcaatat caggatataa attaccattt caatttccct acaaagaaag ccgggcgttt
     1801 ttcttttttt ggcattgccg gcaaagccta tgttgagttg ctcgatagcg aaaaggacac
     1861 cacaaaaaac aacctcttcg attccgaaga gccgcaaaac ggatattttg gaaacagcat
     1921 gggtgtagcc ggtgtgcagc atctgtacat tttcaaaaat aatatgtacc tcaaaactac
     1981 tatggctgtt tcgggtattt cgcaagactg gaaaatagat tcgcttggtg ctgacgatta
     2041 taaaccttat cctttttacc gcaataactc ttacagtata cgtagcacat taaatacaca
     2101 atttcaaaaa aaaattaaca ccaggaatgt ttttaaagca ggaataattg tggagcaata
     2161 caattttcaa tttgccgata gtgttttgga taatgatgag tttcgtatta tctccaaatc
     2221 gaaaggtggc atgcaagtgt atcaggcata tgtacaatgg cagcacaaat tttcggacca
     2281 cagcaccctc aatgccggtg tacattcact tttgctgggc ttaaataaaa cgtattccat
     2341 cgagccccgg ttgggttaca gattcgaatt caatccacgc catgctattt cttttggtgc
     2401 agcattgcat acacaaatgc aacctgttta tttttatttt attgaaacac gtactggctt
     2461 gaatcaattt acacaaacca accgcaactt gtccttaacc aaaagtgcac aagctgtggt
     2521 ggcgtacgat tttaaattcc gaaaagattt cagaataaaa ctcgaaacct attatcagca
     2581 tcttttcgat attcctgttg aaaaaagatc aacttcttat tcgctcataa atgaaggtgc
     2641 cgactttgct ctttctacac gcgatagttt agtgaacaac ggaacggcaa acaataaagg
     2701 gttagaaatc acaatcgaaa aattttatag ccacggatat tattttcttt ttacttcttc
     2761 actattccag tcgttataca aaggaagcga taaggtgacg cgcaatacgg ctttcaacgg
     2821 gcaatatatt tttaatgccc ttgccggtaa agaatggact attaaaaagg acaatgttat
     2881 ttctataaac ttccgttcaa catatgcagg tggcaagcgt tacacaccta tcgatttgca
     2941 gcgaacaata attgaacagg aaacggtgct cgatccgacc agggcataca gtgcacaata
     3001 caaaaattac tttcgcaccg atgtaaagtt ttcgtaccgc atcaatggca agaaagtaac
     3061 acaggaattt tcgctcgaca taaacaatgt attcaatata aaaaatatct ggcaacagca
     3121 atacaatccc aaatcgggaa aaattgtaac cgagtttcag atagggtttt ttcccattcc
     3181 tttgtatcgc atttactttt aaaatattaa aatgaactta tcagaattaa tctggcgcac
     3241 aaaaaaattt gacgaactta ctttagatca actctacgca attttaaagt tacgtatgga
     3301 ggtttttatc gtagagcaaa attgttttta cctcgacctc gatgatgtgg ataaagtttg
     3361 tttgcattta tatgccaccg atagtgccga taatattgcc gcctatgcaa gggcaatacc
     3421 gcaaggtgcc gtatacaaaa gcagttcgtt cggacgagta gtagtgcatc ctcaatggcg
     3481 tggtaccggt cttggaaaat tacttaccaa aaacgtgatg gaggtaatcg aaaataattt
     3541 tggcacaaca gaaataaaaa tatctgcaca atcgcacctc gaaaaatttt atgtttcgtt
     3601 cggctttgta aaatgtggtg atgaatatat ggatgccgga attctgcata tacctatgac
     3661 tattcttcgt ttttagtttt tagttgtttg ttcttcgttt cgttttcgaa gcgccatgtt
     3721 cttcgtcccg acacttgggt cgggatgccg accaaagtgt cggcattctg cgtttcgttt
     3781 gcggggagaa ttgttcgtag ttttgtt
//
LOCUS       DAORQA010000176         3801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5864_length_3801_cov_13.838761, whole genome shotgun sequence.
ACCESSION   DAORQA010000176 DAORQA010000000
VERSION     DAORQA010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5864_length_3801_cov_13.838761"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..>795
                     /locus_tag="PK736_07695"
     CDS             30..>795
                     /locus_tag="PK736_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33312.1"
                     /translation="MQKIIKSILLFLLAFSFGTTQAQLNQIDLPVTFDLTNVDYTVTD
                     FGGNSTVLGPDPTNAANTVAITTKGATAQTWAGTTVGTNLGFANPIPFTANNRKMRVR
                     VYSPDANIPVRLKVEVHGQAGQSVETEAMTTTANAWEWLNFNFNNEAPGTAAFNAAYA
                     YDMASIFFNFGTDGATAGAKTYYFENVKLMQAPPAQIDLPVTFDLANVNYTVTDFGGN
                     STVLGPDPNNAANTVAITTKDVTAQTWAGTTIGTNLG"
     assembly_gap    796..895
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <896..2708
                     /locus_tag="PK736_07700"
     CDS             <896..2708
                     /locus_tag="PK736_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33313.1"
                     /translation="TAGQKTYLFDDVEFVSGALNQIDLPVTFDLANVDYTVTDFGGNS
                     TVLGPDPNNAANTVAITTKDVTAQTWAGTTIGTNLGFASVIPFTAVDRKMRVRVYSPD
                     ANIPVRLKVEVHGQPGQSVETEALTTGVNTWEWLTFDFDNEAPGTAAFNAAFPYDMAS
                     IFFNFGTDGATAGQKTYLFDDVEFVSGANLGLVLPITYDDSTITYTMTDFDGGITTLM
                     TNPYPGGLDTTPKVLRMIKNVGQVWGGSKLTLASALDFTTNNTFKMFVYSPRTGCPVV
                     FKLENSAGGFVEKSDTTTVANAWEELSWDFSGTASNTYTDLVFIYDLGVMGDSSANFT
                     FYQDEIRFVTGGGTGLNQINLPVYFDSLNVDYTLTDFGGNASVLGPDPNNAANKVAIT
                     TKTAAAQTWAGTTIGTNLGFANVIPFTANNHKMRVRVYSPDANIPVRLKVEVHGQPTQ
                     SVETESLTTGVNTWEYLVFDFDNEAPGTAVFNPAYLYDMASIFFNFGTDGATAGQKTY
                     LFDDVELFSSIGLSETPQSSFMLYPNPATTTMHVYVSDNSKLQNAKFEVLDLTGRVIL
                     NGQLNNGVINTSNLVKGIFTLKIITPQGVMNNTFVKQ"
     gene            complement(3178..>3801)
                     /locus_tag="PK736_07705"
     CDS             complement(3178..>3801)
                     /locus_tag="PK736_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRC33314.1"
                     /translation="VPSYIVYVVLAISIGYFLYNAFNDFKNKNMINAIKYIVFSFLAI
                     AFIMMFLNYKGLPVPVLIVLIVALVVSFVMENVPFGRYIFAIGGNREAAFYSGINIRK
                     QIFRVYILMGLLIAIAGIVYTAQLGSATSAAARNLELDVIAACVIGGCSLLGGRGNIF
                     DALCGALIMASLDNGMSLLNVQDYIQDVIKGVILVLAVGIDMLNKKN"
BASE COUNT         1211 a          764 c          667 g         1059 t
ORIGIN      
        1 attcttatca tttcataaaa aaaaaataaa tgcaaaaaat tatcaaatct attctgttgt
       61 ttcttttagc cttcagcttc ggaacaacac aggctcagtt aaatcaaatt gatttgcctg
      121 taacattcga cctcacaaat gttgattata ccgttaccga ttttggtggc aattcaaccg
      181 tgcttggtcc cgatccaacc aatgctgcca atactgttgc cataacaaca aaaggtgcaa
      241 ccgcgcaaac atgggccggc actaccgttg gtaccaatct tggttttgca aatccaattc
      301 catttacagc aaataataga aaaatgagag tgcgtgtata ctcacccgat gcaaatattc
      361 cggttcgatt aaaagtggaa gtacacggac aagcaggcca atcggtagaa acagaagcca
      421 tgacaacaac ggcaaatgca tgggagtggt taaattttaa tttcaataat gaagctccgg
      481 gcacggcagc tttcaatgca gcttacgctt atgatatggc atccatattt tttaacttcg
      541 gtaccgatgg agcaactgca ggagcaaaaa catactactt cgaaaatgta aaattgatgc
      601 aagccccacc agcacaaatt gatttgccgg taactttcga tctggctaat gtgaattata
      661 ccgttaccga ttttggtgga aactctactg tgcttggtcc cgatccaaac aatgctgcaa
      721 acactgttgc tattacaaca aaagatgtaa ccgcgcaaac atgggcaggt actaccattg
      781 gaaccaatct tggttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaactg
      901 caggacaaaa aacttatctc ttcgatgatg tagaatttgt atcgggtgca ttaaatcaaa
      961 ttgatttgcc tgtaactttt gaccttgcta atgttgatta taccgttacc gattttggtg
     1021 gcaactctac tgttcttggt cccgatccaa ataatgctgc aaacactgtt gctataacaa
     1081 ctaaagatgt aaccgcgcaa acatgggcag gtactaccat tggaaccaat cttggttttg
     1141 cttcggtaat tccttttacc gcagtcgata gaaaaatgag agtgcgtgta tactcacccg
     1201 atgcaaatat tcctgtgcga ttgaaagtgg aagtgcacgg acaacccgga caatcggtag
     1261 aaacagaagc attaacaaca ggtgtaaata catgggaatg gttaactttc gatttcgata
     1321 atgaagctcc gggtacggca gctttcaatg cagcatttcc atacgatatg gcttccatat
     1381 tttttaactt cggtaccgat ggtgcaactg caggacaaaa aacttatctc ttcgatgatg
     1441 tagaatttgt atcgggtgca aatcttggat tggtattgcc aattacttat gatgattcta
     1501 caattacata caccatgaca gacttcgatg gtggcataac aacattaatg acaaacccgt
     1561 atccgggagg attggataca acgcctaaag tgctgcgaat gataaaaaat gttggtcagg
     1621 tttggggagg aagtaaatta actcttgcat cggcattaga ctttaccacc aacaacacat
     1681 ttaaaatgtt tgtctattct cctcgcaccg gatgtcctgt tgttttcaaa ttagaaaact
     1741 ctgccggtgg atttgtcgaa aaatcggata ctactacagt agcaaatgca tgggaagaat
     1801 tgagttggga tttttcggga acagcatcaa atacttatac cgatttagtc ttcatttatg
     1861 atttaggtgt gatgggcgat agcagtgcaa actttacatt ctaccaggac gaaattcgtt
     1921 ttgttacggg tggtggcaca ggtttaaatc aaataaacct tccggtatat ttcgattcat
     1981 taaatgtaga ttataccctt accgattttg gaggaaatgc atcggtactt gggcccgacc
     2041 caaacaatgc cgcaaacaaa gtagctatca ctacaaaaac tgcagcagca caaacatggg
     2101 ccggtaccac cattggaaca aaccttggct ttgctaatgt tattcctttc acggctaata
     2161 atcacaaaat gcgtgttcgt gtttactcgc ccgatgcaaa tattccggta cggttgaaag
     2221 tggaagtgca cggacagcca actcaaagtg ttgaaacaga atccctcact acaggagtaa
     2281 atacctggga atatcttgtg ttcgattttg ataacgaagc accaggaact gctgttttca
     2341 accccgcata cttatacgac atggcctcca tattttttaa cttcggtacc gatggtgcaa
     2401 cagcgggaca aaaaacttat ctcttcgatg atgtagaatt attcagctca attggcttat
     2461 cagaaacacc acaatcttcg tttatgcttt atcctaatcc tgcaacaaca actatgcatg
     2521 tatacgtatc tgataattca aaattacaaa atgcaaaatt tgaagtgctg gatttaacag
     2581 gaagagtaat actcaatggc caacttaaca atggcgtaat aaacacaagc aacttagtta
     2641 aaggaatttt tacacttaaa ataattacgc cacaaggtgt aatgaataat actttcgtta
     2701 aacagtaatc gctactcaat aaacttgcaa ggcataaatt gtttgccttg caagtttttt
     2761 tttatttcaa cttactactc ccctatcccc aaaaacaggt tataataaat tacaagccac
     2821 taactttttt tgaaaaacaa caggtctaat tttcaaacac aaattccaaa tttagtttcg
     2881 taaagctttc gagcgtaatt cttattcgtt gtttcacatt ttatgtttgg aattactaag
     2941 ccgtttaaat tataataagc aaataaaatt acagcaccac tgttgacata acctaacaaa
     3001 aatataattt catattatca gaaagagtaa actgacgact tatagttaaa gaacccaaaa
     3061 agcaaaattg actttaacca gaagttgcat ggcttttgaa aaaagactgt ttaggtatat
     3121 aatgccgaaa agcaatcctc tcttttccag cctagcttgt ttctctttgc tggtagttta
     3181 attcttttta ttgagcatat cgattcctac tgctaataca agtataactc cttttatcac
     3241 atcctgaata taatcctgca cattgagaag gctcatgcca ttgtcgagcg atgccataat
     3301 gagtgcgcca cacaaggcat cgaaaatatt tccgcgaccg cccagcaaac tgcaaccacc
     3361 aattacacag gcagcaatca catcgagttc gaggttgcgc gctgctgccg atgttgcact
     3421 gcccagctgc gctgtgtaaa caatacctgc tatagcaatg agcaagccca tcaatatata
     3481 tacacgaaat atttgtttgc gaatatttat accggaataa aatgcagctt cgcggttgcc
     3541 acctatagca aatatgtatc gtccaaaggg aacgttttcc attacaaacg aaaccaccaa
     3601 tgccacaata agcacaatca aaaccggtac aggcaatcct ttataattta aaaacatcat
     3661 gataaaagct atggccaaaa aagaaaatac aatatacttt atggcattaa tcatattctt
     3721 gtttttaaaa tcattaaacg cattatacaa aaaataacca atacttattg ccagtacaac
     3781 atatactata tacgatggta c
//
LOCUS       DAORQA010000177         3786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5897_length_3786_cov_6.910212, whole genome shotgun sequence.
ACCESSION   DAORQA010000177 DAORQA010000000
VERSION     DAORQA010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5897_length_3786_cov_6.910212"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..289
                     /locus_tag="PK736_07710"
     CDS             113..289
                     /locus_tag="PK736_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33315.1"
                     /translation="MSRSKTGRPILGSIPKAFKRLQNKSQKMKLKENSKLMITNAEGN
                     VELRIRKTHSWHWL"
     gene            complement(286..618)
                     /locus_tag="PK736_07715"
     CDS             complement(286..618)
                     /locus_tag="PK736_07715"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRC33316.1"
                     /translation="MYKQADIVVVKFPFTDGSEFKKRPALIVSNEAVNKTGDYLIVQI
                     TSKLNNDDLSIAINDVDCLQPLPLKSYIRIHKIFTVHKSLVLSKITDAKPHFLKSVTD
                     KVCSLIKS"
     gene            complement(622..852)
                     /locus_tag="PK736_07720"
     CDS             complement(622..852)
                     /locus_tag="PK736_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33317.1"
                     /translation="MEVKAIVQEIQRLPLAKRFWVMEQTLKSIKKEELSQQVDTDFDD
                     EKDISFSALSVSEKSLAKDWLSTEDNRWDTIL"
     gene            1032..1196
                     /locus_tag="PK736_07725"
     CDS             1032..1196
                     /locus_tag="PK736_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33318.1"
                     /translation="MPDTLLQIKIGDTLITYIFASRIYNIPNSVSYKVLSATYISEGS
                     QMIAVFGNNN"
     gene            complement(1408..1481)
                     /locus_tag="PK736_07730"
     tRNA            complement(1408..1481)
                     /locus_tag="PK736_07730"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1445..1447),aa:Pro,seq:ggg)
     gene            complement(1540..2337)
                     /locus_tag="PK736_07735"
     CDS             complement(1540..2337)
                     /locus_tag="PK736_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRC33319.1"
                     /translation="MTVYVDQLNRQVNIKSSPQRIVSLVPSQTELLFDLGVADRVVGV
                     TKFCIHPAEAIATTTIVGGTKQIKHDVIKALQPDLIIANKEENTKEDLELLMQHYPVW
                     ISDINNLNEVFSMIANVGEIIGRENEAKKLNSEITKGAAILAEKATSFKTRKRVAYFI
                     WYKPWMVAANDTFIHSMLELCGWQNVFEKQERYPSVEIEQLAKLKPDIILLSSEPFPF
                     KTKHVDMLKTICPQAQVILTDGEMWSWYGSRLTKALPYLCETISRFE"
     gene            complement(2364..>2656)
                     /locus_tag="PK736_07740"
     CDS             complement(2364..>2656)
                     /locus_tag="PK736_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HRC33320.1"
                     /translation="ITENDYSQIPVTENKRIMGAISENHIFNEMVKNPDIHHQPIKNI
                     MQTALPFVDISTSLDALSAMINAENNAVLVRDFKSDSTYIITRHDIMQALAK"
     assembly_gap    2657..2703
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2704..3725)
                     /locus_tag="PK736_07745"
     CDS             complement(<2704..3725)
                     /locus_tag="PK736_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRC33321.1"
                     /translation="MKVCNNILEAIGNTPMVRLNKVTESVSATVYAKVETFNPGNSIK
                     DRMAVKMVEDAEKAGLLKPGGTIIEGTSGNTGMGLAIAAIVKGYKCVFTTTDKQSKEK
                     VDALKAFGADVIVCPTNVEPEDPRSYYSVSSRLAKEIPNSWKANQYDNLSNRMAHYEQ
                     TGPEIWDQTDGKITHLVVGAGTGGTISGTGKYLKEKNPNIKVWAIDTYGSALKKYHET
                     GVLDQNEIYPYITEGIGEDFIPENYDFKVIDHFEKVTDKDAAVMARELVAKEGIWVGY
                     SAGSAVMGVLQMKDQLKPTDVVVVIFHDHGSRYIGKLFNDNWMREKGYLDKKGFTAKD
                     LVGDSAR"
BASE COUNT         1118 a          738 c          658 g         1225 t
ORIGIN      
        1 ggtttataac cctgcaaaca aatagcatcc agatgttttg caacaaattt atgatgagac
       61 aatacaaata gtaacaaccc ggtttaatcg aatttttaca attattaaaa aaatgagcag
      121 aagtaaaaca ggaagaccaa tattagggag tattcccaag gcatttaagc gtttgcaaaa
      181 taagagccaa aagatgaagc taaaagagaa tagcaaatta atgattacaa atgccgaagg
      241 taatgttgag ttgcgtattc gtaaaaccca ctcctggcat tggctttaac tttttatcaa
      301 actacaaact ttatcggtaa ccgatttaag aaaatgaggt ttagcatccg taattttaga
      361 gagtactaag ctcttgtgaa ctgtaaatat cttgtgtatt cggatataac tttttagtgg
      421 taagggttgc aaacaatcga catcattaat agctatagaa agatcatcgt tattgagttt
      481 agaagtaatt tgtacaatca aataatcgcc agttttattt acggcttcat ttgaaacaat
      541 tagggcagga cgttttttaa attcagaacc gtcagtaaaa ggaaacttga ctactacaat
      601 atcagcttgt ttgtacatag gttataaaat tgtatcccaa cgattatctt cggtagatag
      661 ccaatctttg gctaatgatt tttcacttac tgaaagagcg gaaaaagata tgtctttttc
      721 atcgtcaaaa tcagtatcta cctgttggct caactcttct ttcttaatag actttaaagt
      781 ttgttccatt acccaaaatc gttttgctaa gggtaaccgt tgaatttctt gaactattgc
      841 ttttacttcc attgtattaa ttattaaaat gctaacttat aagcgagcgt ctgtttttat
      901 ttctgtttgc ttcatatcaa tttttcaacc cgttattttt tacaaatata caaaaataat
      961 tattacaacc tgtagagtaa tgtgttacaa catacataca agtatttgct atacattcgc
     1021 tcaatttaca aatgcccgat acgcttctac aaataaaaat tggcgacaca cttattactt
     1081 atatatttgc ctcacgaata tacaatatac caaacagcgt atcatacaaa gtgctatcag
     1141 caacatacat aagcgaaggc tcacaaatga tagcagtatt cggcaacaat aactaaatca
     1201 agaatatatc cgcaaacgaa tttattaaac acataaccta ttttcgatat tgaaatgcta
     1261 caagggttgt aacatgacta aaaatattgt tgtaaaatgg ttttactcat tgcgttgttt
     1321 gcactaacgg taataatata cgtttaaaaa caataaagta ctttttgggc aaaaaaaaat
     1381 cccgcacaag caggattaaa ttttttgtcg gggtgactgg attcgaacca gcgacctcca
     1441 gcaccccatg ctgatacgct accggactgc gctacacccc gaactattgg tgggcggcaa
     1501 aactaattat tgttatcaat aaaaaaaacc aaaaaggttt tattcgaacc tggaaattgt
     1561 ttcacataag taaggtaatg ctttggttaa cctcgaacca taccagctcc acatttcgcc
     1621 atcggtcaat attacttgag cctgagggca gatagtttta agcatgtcca cgtgttttgt
     1681 tttgaacgga aagggttcgc tcgacaaaag aataatatca ggctttaatt ttgccagttg
     1741 ttctatttcc acagaggggt aacgctcttg cttttcaaat acattttgcc aaccacatag
     1801 ttcaagcatc gaatgtatga atgtatcatt ggcggccacc atccatggtt tgtaccaaat
     1861 aaaataagca acacgctttc gtgttttgaa tgaagttgct ttctcagcta aaattgctgc
     1921 accttttgta atttccgaat tcagcttttt cgcttcattt tctctcccaa taatttcccc
     1981 aacattagca atcatagaaa acacttcatt caaattattt atatcgctta tccataccgg
     2041 ataatgttgc atcagcaatt ctaaatcttc tttggtgttt tcttctttat tagcaataat
     2101 caaatcgggc tgtaatgctt tgattacatc atgcttaatt tgttttgttc caccaactat
     2161 tgtagttgtg gcaatagctt cggcaggatg aatgcaaaat tttgttacac ccactactct
     2221 gtctgcaact cccaaatcga aaagtaattc ggtttgagat ggcactagtg atacaatccg
     2281 ctgtggtgat gacttaatgt ttacctgtcg gttaagttgg tcaacgtata cagtcatggt
     2341 taaaactaaa tttcgaaaaa taattatttt gccagtgcct gcataatatc gtggcgggta
     2401 ataatgtaag tgctgtcgga tttaaaatcg cgaacaagca cagcattatt ttcggcattt
     2461 atcatggccg acaaagcatc gaggctagta gaaatatcaa caaaaggcaa agcagtttgc
     2521 ataatatttt ttataggttg gtgatgtatg tcgggatttt ttaccatttc attaaaaata
     2581 tgattctcgc tgatagcgcc cattattcgt ttgttttcgg taacaggtat ttgcgaataa
     2641 tcgttttcgg taattannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnncgcgcgc tatcgcctac caaatccttt gcggtaaaac cttttttatc gagatatcct
     2761 ttttcgcgca tccagttatc gttaaataat ttaccaatat agcgcgaacc atgatcgtga
     2821 aaaataacaa caacaacgtc tgtaggttta agttgatctt tcatttgcaa cacgcccatc
     2881 acagcactgc cggcactgta gccaacccat atgccttctt tggccaccaa ctcgcgcgcc
     2941 ataactgctg catctttatc ggtcactttt tcgaaatggt ctatcacttt gaaatcgtaa
     3001 ttttcgggaa taaaatcttc gcctatgcct tcggttatgt aaggataaat ttcgttttga
     3061 tcgagcacac ccgtttcgtg atattttttc aaggccgaac catatgtatc tattgcccac
     3121 acttttatgt tgggattttt ttctttcaaa tattttcctg tgcccgatat agttccaccc
     3181 gttcctgcac ccacaacaag atgggtaatt ttcccatcgg tttggtccca tatctcaggg
     3241 cctgtttgct catagtgcgc catcctgttc gataaattat cgtattggtt tgccttccac
     3301 gaattgggaa tttcttttgc caaccgtgac gataccgagt aatatgaacg tggatcttcg
     3361 ggctcaacat tggtggggca aacaattaca tcggcaccaa aggctttcag cgcatcaact
     3421 ttttctttcg attgtttatc ggtggtggtg aatacgcatt tgtatccttt tacaatggca
     3481 gcaattgcaa ggcccattcc tgtattgccg cttgtgcctt ctatgatggt gccaccgggt
     3541 tttagcagtc cagccttctc ggcatcttca accattttta ctgccatgcg atcttttatc
     3601 gaattgcccg gattaaatgt ttcgaccttg gcatacaccg ttgccgatac cgattcggtt
     3661 actttgttga gacgcaccat tggtgtattt cctatcgctt cgagaatatt attacaaacc
     3721 ttcatcactt tttattttgg tcggcaaaag taaaattaat ggtggggatt gcaagggtgt
     3781 gtaaac
//
LOCUS       DAORQA010000178         3784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5898_length_3784_cov_12.744167, whole genome shotgun sequence.
ACCESSION   DAORQA010000178 DAORQA010000000
VERSION     DAORQA010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5898_length_3784_cov_12.744167"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="PK736_07750"
     CDS             <1..1291
                     /locus_tag="PK736_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HRC33322.1"
                     /translation="DSIVNYKISSFIAGNEDKTYSFTALSKFKVSLETINDTIKISRI
                     ERLEVLKFDCTNDRDNDGVLDDEDVCPYLKGDITAQGCPDFDMDGVADNKDECDYIYG
                     DVKNHGCPTNYFTNKVSFAFIVGAQANSVNMNLPELDKTGYNNLDFNESQKGKIKNPD
                     LIPSPVFGAELSYYFGKKKKTTGFSLGMLYTTFKANYQIDEPTVYTFRASDGIDDYRR
                     RITLKAGSEEEINYRILNMPLLIKWRWMKTKTKASEYKVSFEFSAGPSFMMFQNSSKY
                     NYNVDFEGLYQIDTITKNKIVYYDYFNNSSTYNVMVTANNINQQSSIPGSDIVFGQLN
                     DRQYDFASNKNIKGTDKKVNRNTIGINAKFDGYYRMAKHTSFKFGAGVVYAPLFAGPS
                     GYKAIDKTTDEFNSIYNAQAQAYYFAFALNMGFVIHW"
     gene            1657..>3784
                     /locus_tag="PK736_07755"
     CDS             1657..>3784
                     /locus_tag="PK736_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33323.1"
                     /translation="MISLRTLITTILGFGFFFANAANINWINTGGGNWSVGTNWSGGA
                     APGVNDTAVINENGTYTVTVDVNIGVAKIVVGGGTGKQKLMADNRTINVSSAIVINAG
                     HTMHCNNNNIINGAGSITNNGVLLLVYNNALNVNVLNNDSIIIQNSYNTCAGIYTCTA
                     PSVLHFDAQALTSLTFATGWTNNGKILISSPGCQTATLAITTGVLINSPSGIITINPN
                     PGCYGYYNLNCGINNQGTINFNYITNWDVSSGASVNTGVINIAAGVTVTVKNGTLSSS
                     NTGTFNFTSATSKLNFSSATYTNTGAVYNGNGTIRFDYSTANLNVDTIKNIPLYFYQS
                     TINSNKPTVVNKVRMTIEYANTINANINNNDSIIVINSSNTMGGAYTCTSNSILHFDA
                     QGFPTLTFANGWTNNGKILMTSPGCYPSTLSITTGVLINPPSGIITIDPNPGCYGYYN
                     LTCGINNQGTININYITNWDVSSGASVNTGVINIAAGVTVTVKNGTLSSSNTGTFNFA
                     SATSKLNFSYATYTNTGAVYNGNGTMRFDYSIANLNVDTLKNIPLHFYYSTINSNKPT
                     VVNKVRMTIEYANTINANINNNDSIVILNSSNTMSGAYTCTSNSILHFDAQGFPTLTF
                     ANGWTNHGKILMTSPGCYSSTLTITTGVLINPPAGIITIDPNPACYGYYNLTCGINNQ
                     GTINFNYNTNWDVSSGASVNTGVINIA"
BASE COUNT         1292 a          708 c          677 g         1107 t
ORIGIN      
        1 tgatagtata gtaaattata aaataagcag ttttattgcc ggtaacgaag ataagactta
       61 cagttttaca gcattaagta aatttaaagt aagtttagaa actataaacg ataccatcaa
      121 aatttcgaga attgaaagac tcgaagtgtt gaagtttgat tgcaccaatg atcgtgataa
      181 tgatggtgtg cttgatgatg aggatgtttg cccctacctg aaaggggata ttaccgcaca
      241 gggttgtccc gatttcgaca tggatggtgt ggccgataat aaagacgaat gtgattatat
      301 ttatggcgat gtaaaaaatc acggttgccc tacaaattat tttacaaaca aagtgagttt
      361 tgcattcata gttggtgcac aggcaaattc tgtaaacatg aatttacccg aactcgataa
      421 aacaggatat aataatttgg attttaatga atcgcaaaaa ggaaaaataa aaaatcccga
      481 ccttatacca agcccggttt ttggtgccga actctcctat tattttggca agaaaaaaaa
      541 gacaacagga ttttcgctcg gcatgcttta cacaactttt aaagcaaact accaaataga
      601 cgaacctaca gtttacacct ttagggcaag tgatggcata gatgactatc gcaggcgcat
      661 tacattgaaa gccggatcgg aagaagaaat aaattaccgt attttaaata tgcctttgct
      721 gattaaatgg cgatggatga aaactaaaac aaaagcatca gaatacaaag tgtcgttcga
      781 attcagtgcg gggccatctt tcatgatgtt tcagaatagt tcgaagtaca attacaatgt
      841 tgattttgaa ggcctgtacc aaatcgatac cattaccaaa aacaaaatag tgtattacga
      901 ttatttcaac aatagttcaa cttataacgt aatggttacg gcaaacaata tcaatcagca
      961 atcgagcatt cccggtagcg atattgtttt tggccaactc aacgataggc aatatgattt
     1021 tgcaagcaat aaaaatatta agggaaccga taaaaaagtt aatcgcaaca ccataggcat
     1081 caatgccaaa tttgatggtt attaccgtat ggccaaacat acttcgttca agtttggtgc
     1141 aggggttgtt tatgctccat tatttgccgg accatcagga tataaagcca tagataaaac
     1201 taccgatgag tttaattcta tttacaatgc acaagcgcag gcatattatt ttgcctttgc
     1261 cttaaatatg ggttttgtaa ttcactggta agcaaaaaat agtttcggtt tttttatagc
     1321 atccatggta aatattttta tcatggagta ttgaacttta catttccgtt ttttcagaac
     1381 tgaccattgt gccagctttc caataaaaac agaatagcac attgccattg attacaaaaa
     1441 aatcttgact gtgcttttta caaacgtgtt ttttgttttt tataaatgat gtccactccc
     1501 gaataatagt ctttcaactc tacattatgt atgttggtaa aattgtcgaa aagcaattca
     1561 ttttttacca tttataaaga gaaataattt aattttgaag aattggtttt agaacctttt
     1621 gccctttttt aaatatactt tttaaaacac taagagatga tttcactacg taccttaatc
     1681 acaacaattt taggtttcgg attctttttt gccaatgcgg caaatattaa ctggataaat
     1741 actggcggtg ggaactggtc ggtgggcaca aactggagtg gcggtgctgc acccggagta
     1801 aacgacacag ccgttattaa tgagaatggt acgtatacag ttaccgttga tgtaaatatc
     1861 ggagtggcta aaattgtggt tggtggcggt accggcaaac aaaaactgat ggccgacaat
     1921 cgcaccatca atgtttcaag cgccattgtt ataaatgcag gacatacaat gcactgtaat
     1981 aataacaaca taataaatgg tgcgggaagt attacaaaca atggagtgct attattggtt
     2041 tacaacaatg ccttaaatgt aaatgtgctt aacaatgatt ctataattat tcagaatagc
     2101 tataatactt gtgcaggaat ttacacatgc actgcacctt cggttttaca tttcgatgca
     2161 caggcattaa catctcttac atttgccacc ggctggacaa acaatggaaa aatattaatt
     2221 tccagtccgg gatgtcaaac ggcaacgctt gcaatcacaa caggtgtatt aataaattca
     2281 ccctcaggca ttataactat taaccctaac ccgggatgtt atggatatta caatctcaac
     2341 tgtggcatca acaatcaagg cacaataaat tttaattata ttacaaactg ggatgtaagc
     2401 tcaggggcat cagtaaatac cggtgttatt aatattgcgg caggtgtaac ggttaccgta
     2461 aaaaatggaa cgcttagcag ttcgaatacc ggcaccttta attttacaag cgcaaccagt
     2521 aaattaaatt tttccagtgc cacctacaca aataccggag ctgtgtataa tggtaatggt
     2581 actatcaggt tcgattattc aactgcaaat ttaaatgtcg acacaataaa aaatattcca
     2641 ctatactttt accaatctac aataaattcg aataaaccca ctgttgtaaa taaagtacgt
     2701 atgacaattg aatatgccaa tacaattaat gcgaacataa ataataacga ttcgataatt
     2761 gtgatcaaca gctccaatac catgggtggt gcttacacat gcacttctaa ctctattcta
     2821 catttcgatg cacagggatt cccaactcta acatttgcca acggctggac aaacaatgga
     2881 aaaatattaa tgaccagccc gggatgttac ccatcaacgc tttcaatcac cacgggtgta
     2941 ttaataaatc caccctcagg cattataact atagacccta acccgggatg ttatggatat
     3001 tacaatctca catgcggcat caacaatcaa gggacaataa atattaatta cattacaaac
     3061 tgggatgtaa gctcaggggc atcggtaaat accggtgtta ttaatattgc tgcaggcgta
     3121 acggttaccg taaaaaatgg aacgctcagc agttcgaata caggcacctt taactttgca
     3181 agcgctacga gtaaattaaa tttttcttat gccacctaca caaataccgg agctgtgtat
     3241 aatggtaatg gtactatgcg gttcgattac tccatagcaa atttaaatgt ggacacacta
     3301 aaaaatattc cattacactt ttactattct accataaatt cgaataaacc cactgttgta
     3361 aataaagtgc gtatgacaat tgaatatgcc aatacaatta atgcgaacat aaataataac
     3421 gattcgattg ttatactcaa cagctccaat accatgagtg gtgcttacac atgcacttca
     3481 aactctattt tacatttcga tgcacaggga ttcccaactc ttacatttgc caatggttgg
     3541 accaaccatg gaaaaatatt aatgaccagc ccgggatgtt actcatcaac acttacaatc
     3601 accacaggag tattaataaa tccacctgca ggcattatta ccatcgaccc caatccggca
     3661 tgctacggtt attataacct cacatgcggc atcaacaatc agggaaccat aaattttaat
     3721 tacaatacta actgggatgt aagctcaggg gcatcggtaa ataccggtgt tattaatatt
     3781 gctg
//
LOCUS       DAORQA010000179         3783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5902_length_3783_cov_6.433476, whole genome shotgun sequence.
ACCESSION   DAORQA010000179 DAORQA010000000
VERSION     DAORQA010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5902_length_3783_cov_6.433476"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..2566)
                     /locus_tag="PK736_07760"
     CDS             complement(68..2566)
                     /locus_tag="PK736_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HRC33324.1"
                     /translation="MKKILFKICVAVSMLLASNNLLTAQSNQYLDFDGVDDFVEAPNA
                     SALIAGSTAMSITGWFYDNALGYGQGMMGYRNTNAGFYLIILNNGAIECRMQNSAGTL
                     VTYQTPDFTVVPQVWQPYSWGYWGGQLQRNRNGNLVGGKPASGSIASTTTSFAIGKSI
                     LGNFNFIYNGRIDEVSVWNKELTQTEIQDMMTNELLGTEPNLQLYYKFDQGVPGGNNS
                     SISSLTTNVNGPQYNGVFNNFALNGATSNFNGTLNSSFQAISFPQVPTQLITQPSYYL
                     NATATSGLPCTYTVLSGPATISNDTAYFSGVGTVSIQADQFGNGTYDTAVAVISTFDV
                     VDPYATQAVIEPRNPVQGADIYMPTLGALQLSVVANMPYPTIFSVSNLEFVINGTTYP
                     AQSFANGRYSAWWTPNAFGAQTIQINSTSNYGVVTSVTVNVNVVQTVSTLTNVPAFSG
                     LWLNSTTGVLTADGVLPSYVGAFDTIIATLSVTCPPNGGCDPWDRVSSIDVMGMDGLW
                     YEVIRYITPYGVACNHSINMADYMSILQGKVTFRVNLGTLTNGYVYALKFDYKDGAPP
                     HKYSKVEKVWKAIYPFGDMANLQPVPAFAHTFEPSAVAAKLKLVSTGHGWGNLNTGNA
                     AEFYDATHTLWVNGANTFSQHNWTTCSPNPDACQPQNGTWTYARAGWCPGAIGRPFDF
                     DMSTFVANPAINLEYKFLSSYVDQCHPNNPNCVTGVTCTDCNDGFNPTLDVNCNLITF
                     FDDATILSVDEKDDANLIIYPNPSNGIFNLNSVKLTGETYIVTVYNIMGTTVRQFKWD
                     GEKTVLDLTNAAKGVYIVKVNNDTHTEIKQLIVR"
     gene            complement(2837..>3783)
                     /locus_tag="PK736_07765"
     CDS             complement(2837..>3783)
                     /locus_tag="PK736_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HRC33325.1"
                     /translation="KEPVLAAKIIKDIIENNRPALGVNDFGQLIIDPYTESAALYPYQ
                     LGYRNESKGWSFNQSKDVRMGTTMWHLLSKNDSADGSGIFDPRAYYFFETNNFNKWAP
                     FPNNPQSPLQPDGGIPYEYQRDVAYSIKGATCFYSPVNYYLARDMDYQPDILMTGAEV
                     LYLRSEAYLRGIGVAKDPGLATVAFLDGIQYSLNFWQHVMDNSKLPPGTSFVTNITVP
                     SNLDFISVQNNIGFFNGDEAEQLREIYAQTWIDQMRQPQEAFALARRTLNTPREGDPI
                     SVYRFPIPPSETTYNQLNWLNAIGNAGDDLTQKVWWMN"
BASE COUNT         1122 a          752 c          745 g         1164 t
ORIGIN      
        1 gtttaaggca caaaaaaaac gcagaagaaa atcctctgcg tttttttagt aaaataaaaa
       61 atctgaatta gcgcactatc agttgtttta tttcggtgtg agtatcgtta tttactttta
      121 caatgtaaac tccttttgct gcattggtaa ggtctagcac tgttttttca ccatcccatt
      181 taaactgacg aacggtagtg cccataatgt tatatacggt aactatatac gtttctccgg
      241 ttaactttac cgagttaaga ttaaatattc cgttagaagg attaggataa ataatcagat
      301 tggcatcgtc tttctcatcc actgatagaa tagttgcatc atcgaaaaat gttatcaaat
      361 tacaattaac atccagtgta ggattaaagc catcgttaca atcggtgcag gtaacaccgg
      421 ttacacaatt gggattgtta ggatgacatt ggtctacata gctcgacaaa aatttgtatt
      481 cgagattgat agcagggtta gcaacaaaag tgctcatatc aaaatcgaat gggcggccga
      541 tagcgcccgg gcaccatccg gcacgtgcat aagtccaggt gccattttgt ggttggcatg
      601 catcaggatt tgggctacac gtagtccagt tgtgttgcga aaatgtattg gcaccattca
      661 cccacaatgt atgtgtagca tcataaaatt ctgccgcatt accggtattt aaatttcccc
      721 aaccatggcc ggtacttacc aatttcaatt ttgccgctac tgcacttggt tcaaaggtat
      781 gtgcgaatgc tggcacaggc tgtaagtttg ccatatcgcc aaatggataa atggctttcc
      841 atactttttc cactttgctg tatttatgtg gtggcgcacc gtctttgtaa tcgaatttta
      901 atgcatacac ataaccgttt gtaagtgtac caaggtttac acggaaagtt actttaccct
      961 gaagtatact catatagtcg gccatattta tcgaatgatt acaagcaaca ccataaggag
     1021 tgatgtaacg tattacttca taccagagcc cgtccattcc cattacatca atgctcgata
     1081 ctctgtccca agggtcgcaa ccaccgtttg gcggacatgt tacagacaag gtagcaataa
     1141 tggtgtcgaa tgccccaaca taagaaggta atacgccatc ggccgttagg acccctgttg
     1201 tggagttaag ccataagccc gaaaatgccg gcacatttgt aagtgtcgat accgtttgta
     1261 ctacgtttac attcaccgtt acagaagtta ctacaccata attggatgta gaatttattt
     1321 gaattgtttg tgcgccaaaa gcattgggcg tccaccatgc cgagtagcgt ccgtttgcaa
     1381 aactttgcgc aggatatgtt gtaccattaa ttacaaattc aaggtttgaa acagagaata
     1441 ttgttgggta aggcatgttt gccaccaccg acaattgtaa agcacctaaa gtaggcatat
     1501 aaatatcggc tccttgcaca gggttgcgag gttcaattac tgcttgtgtg gcatagggat
     1561 ctaccacatc aaatgtgctg ataactgcaa ctgcggtatc gtaagttcca ttaccaaatt
     1621 gatcagcttg tattgaaact gtaccgactc ccgaaaaata tgctgtatcg ttacttatag
     1681 ttgcagggcc cgacaataca gtatatgtac aaggcagtcc cgaagttgca gttgcattaa
     1741 ggtaatagga tggctgagta ataagttgag ttggtacttg aggaaatgat atcgcctgaa
     1801 aagaagaatt cagtgtgcca ttgaaatttg atgtggcacc attgagtgca aagttgttaa
     1861 acactccatt atattgtggt ccgtttacat tggttgtcag gctgctgata cttgaattgt
     1921 ttccaccggg aacaccctga tcaaatttgt aatacaattg aagatttggc tctgttccca
     1981 aaagttcgtt agtcatcata tcctgaattt cggtttgtgt aagttctttg ttccaaaccg
     2041 atacttcatc tattcggccg ttatatataa agttgaaatt gcctagaata cttttgccta
     2101 ttgcaaacga agttgttgta cttgctatac taccgctagc gggctttcca ccaaccaaat
     2161 ttccatttcg atttcgttgg agttggcccc cccaataacc ccaggaataa ggttgccaca
     2221 cttgtggtac tactgtgaaa tcgggtgttt ggtaggtaac aagagtgccg gcagaatttt
     2281 gcatcctgca ctcaatggct ccgttgttca atattattaa ataaaaacct gcattggtgt
     2341 tgcggtaacc catcatgccc tgaccatagc ccaatgcatt atcataaaac caacctgtta
     2401 tactcattgc tgtagaccca gcaataagtg ccgaagcatt gggtgcctca acaaaatcgt
     2461 caacaccatc aaaatcgagg tattgatttg actgcgctgt gagcaaattg ttcgaagcta
     2521 aaagcatcga tactgcaaca caaattttaa atagaatttt tttcatttta atttattgtt
     2581 taattgattt gattattatt tatgggatat tattttgcaa atatattttt aatagttgaa
     2641 ttatttttgc ctcacttatt ccgttattga tatttaataa cacaagtata aaatcattta
     2701 aacatgaacg ctgtcaaaag atattagctt gctttagcta aaatgttgac tcagaattaa
     2761 aaaaaaactc aaactgaaaa cgatgaacaa ataactaaaa acctgaaact ttaaacgatg
     2821 aactttaaac taaaaactaa ttcatccacc atactttttg tgttaaatca tcgccagcat
     2881 ttccaattgc attaagccag ttaagctgat tgtatgttgt ttcggatggc ggaatgggaa
     2941 agcgataaac agaaatggga tcaccttcgc ggggcgtgtt taaagtcctt cttgccaaag
     3001 caaatgcttc ttgtggttgg cgcatttggt caatccaggt ttgagcatat atctcgcgca
     3061 attgttcggc ttcatcacca ttaaaaaatc caatattgtt ttgtaccgaa ataaaatcga
     3121 gattagatgg tacggttatg tttgttacaa acgatgtgcc cggaggaagt ttggagttat
     3181 ccataacgtg ttgccaaaag tttaacgaat actgtatacc atcgagaaaa gccactgtag
     3241 ccagaccggg atcttttgca acaccaatac ctcgtaagta tgcttcggat cttaaatata
     3301 atacctcagc tccggtcatt aaaatgtcgg gttgataatc catatcgcgc gccaaataat
     3361 aatttactgg cgaataaaaa cacgttgcgc cttttatact gtatgccaca tcacgttggt
     3421 actcgtatgg aattccacca tcgggttgca aaggtgattg agggttgttg ggaaatggcg
     3481 cccacttatt aaaattattt gtttcgaaaa aatagtatgc acggggatca aatattccac
     3541 tgccatcggc actatcattt ttcgaaagca aatgccacat ggttgttccc atgcgtacat
     3601 ccttcgattg attgaacgac caacctttgc tttcatttct atacccaagt tgataaggat
     3661 ataaggccgc actttctgta tacggatcga taatcaattg gccaaaatca tttactccca
     3721 atgcaggtct attattttca atgatgtctt taataatttt tgcggccagc acaggttctt
     3781 tat
//
LOCUS       DAORQA010000180         3775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5916_length_3775_cov_6.552419, whole genome shotgun sequence.
ACCESSION   DAORQA010000180 DAORQA010000000
VERSION     DAORQA010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5916_length_3775_cov_6.552419"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="PK736_07770"
     CDS             complement(<1..1065)
                     /locus_tag="PK736_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HRC33326.1"
                     /translation="MDLFERLKASDQGHLGKWSEEADGYFMFPKLEGELGPRMQFNGH
                     EVINWSINNYLGLANHPEVRAADSKAAEDWGMAATLPSNWVVATASYNTTYPIVTAGS
                     STPSTSQANGNNCYAGRASTSSSDYSILQKQATSGSTSFTFSGVNNTASILDGFVIAW
                     NVEQFTLAGRATTVDFTYRINAATYGSTGITGTTLFTAPTGTTAPAFILTSTPRSITI
                     TGLSIPASATVDFRFTIANGAGSGNNAHIGIDDFTLYATSAAVIAPSITSALTASGTC
                     GNPFNYTITATNSPTSYNAINLPAGLSVNTSTGAITGSPTATGIFNVDITATNAAGSD
                     TKTLVLTINPTISFAVSSDNI"
     gene            1231..>3775
                     /locus_tag="PK736_07775"
     CDS             1231..>3775
                     /locus_tag="PK736_07775"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HRC33327.1"
                     /translation="MSFNLENSAPNCNYDNTKKTVREAINQLNNKNIRSMRLLFTLFF
                     AIFLTTFSSAQYSITGTGAGNTYTQDFDAFAGTTITVPANWVWSWNDFTPGGYYNRSA
                     TYSNANSTHALREGASSTDVAFGLKNDNTAKTFTFSVKNNTSAPITGFELNWQVEQYS
                     AFTNATTVSFSYRLNMDTFKLKNASGSGIAAVIASTLPTAANLPSITVTAKTLSISSI
                     SLAVGENADFRFTLGNGSASNAHIGIDDFTLYATSAAVVAPSITSALTANGTCGNPFN
                     YTITATNSPTSYNAINLPTGLSVNTSTGAITGSPTATGIFNVDITATNAAGSDTKTLV
                     LTINPTISFAVSSDNISENGGSYVLTLNGCGTGAYGVNVSVTGGTAPLSDYTFVSPTT
                     VSFSGSGIQVINIPIVNNAVVDGNRTITFGLSSFTGSAVVGSNASFSLTIVDDEIPAP
                     VITHTVIPNTTSITAQVGIASVTNSPSSVIATYRRNGGGWGTVNATFVSGINYSFTIP
                     GMPQGSTIEYYITATNSGGTDLKPSPTTYYSYTVGCTPPAAGTKIIAFQGFEFPETGY
                     TGITVPNQSATPTGLQNYLPHPSLVEMNYYLRGTNGVPLPANRNSCGCMAETFVTGPP
                     PSTFYDNGVNGALNSNTICRNFSGSQRGVYKNGGGSPSTDNPAMPGSDGFARNGNYAY
                     VQQSRNDGGEGPISKIYFDAIQIPDFANATNVKIKLYVSSISTNGNGDEDDFVRAFAR
                     YGATAASATIVNNLASSSDWFNASTNSLVSTTVTGTLSNSERWDYLGNMYNTTGTITG
                     TTTKNIIELSIPAGNEYVRLMIDILNDDSNNRELWAIDDIQIIADYTTTA"
BASE COUNT         1141 a          770 c          781 g         1083 t
ORIGIN      
        1 tatgttatca cttgacacag cgaaggaaat agttggatta atggtcaata ccaatgtttt
       61 tgtatcggac cctgcggcat tggtagcagt aatatcaaca ttgaatatgc ccgttgcagt
      121 tggcgaacct gtgatagccc ctgtgctggt attgacactt aatcctgccg gcaggttaat
      181 ggcattgtag ctcgtaggtg agttggtggc ggtgatggta taattaaatg ggttaccgca
      241 ggtaccgctt gctgtcaatg cacttgtaat agagggggca attactgcag ctgaagtggc
      301 gtagagggta aagtcatcga tgccgatatg ggcattgttg ccggaaccag ctccgtttgc
      361 aatggtaaac ctaaaatcta ctgtagctga tgcgggaata gataaacctg ttatagttat
      421 agaccggggt gtagatgtca aaataaatgc aggtgcagtt gtgcctgtag gtgcagtgaa
      481 cagcgttgtt cctgtaattc ctgtagaacc ataggtagct gcatttattc tataagtaaa
      541 gtccactgta gtggccctac cagccaatgt aaattgttct acattccacg caatcacaaa
      601 tccatctaat atagaagcgg tattattaac cccactaaat gtaaaggaag tagaccctga
      661 agtagcttgt ttctgtaaaa ttgaataatc gctactgctg gtacttgccc ttccggcgta
      721 acaattattc ccattcgctt gactagtact tggtgtagat gaacctgcag ttacaattgg
      781 ataagtagtg ttataagatg ctgtggcaac aacccaattg cttggtaaag tggctgccat
      841 gccccagtcc tcagctgctt tcgaatctgc ggctcttact tcagggtgat tggctagtcc
      901 taaatagtta ttgatactcc agttgatcac ctcatgacca ttgaattgca ttcttggtcc
      961 tagttcacct tccagtttag gaaacataaa atatccatct gcttcttcag accatttacc
     1021 taaatggccc tgatcagaag cttttaatct ttcaaataaa tccatgtgtt atttatatta
     1081 atccgcaaag gtggtaaaaa atgaggaaat atggaaaaga aatagtggta ggcgctctaa
     1141 cattgatcat tgatttaata aaagattaca gaatttattt caaataagga accgcgacaa
     1201 atttatttat actcttttaa cctcaataat gtgagcttca acctcgaaaa tagtgcacca
     1261 aactgcaatt atgacaacac caaaaaaact gttagagaag ccattaatca actgaacaac
     1321 aaaaatatta gaagtatgag gttattattt acactctttt ttgccatttt cttaactaca
     1381 ttttcaagtg ctcaatattc cattaccggc acgggcgcgg gtaatacgta tacgcaggat
     1441 tttgatgcat ttgctggaac gaccataaca gtacctgcaa attgggtttg gtcttggaat
     1501 gattttactc caggtggtta ctacaataga agcgctacat atagtaatgc taattcaact
     1561 catgcgttgc gtgaaggtgc ttcttcaaca gatgttgcat tcggtttaaa aaatgacaat
     1621 accgccaaaa cattcacttt ttcagtgaaa aacaatacct ctgctcctat cacagggttt
     1681 gaactcaatt ggcaagtgga gcaatactcg gcatttacaa atgctactac cgtttctttt
     1741 tcttatagat taaacatgga tacctttaaa ttgaaaaatg caagcggaag tggaatagca
     1801 gccgttatcg caagcacatt gccaactgct gctaatttac cttctattac tgtcactgcg
     1861 aagacattga gtatttcgag tatcagcttg gctgtaggag aaaatgcgga ttttcgattc
     1921 actttaggta atggcagcgc cagcaacgcc cacatcggca tcgatgactt tactctatac
     1981 gccacttcag ctgcggtagt tgccccatct attacaagtg cattgacggc taatggaacc
     2041 tgtggcaacc cttttaatta taccatcacc gccaccaact cacctacgag ctacaatgcc
     2101 ataaatctac ccacaggatt aagtgtcaat accagcacag gggccatcac aggttcgcca
     2161 actgcaacag gtatattcaa tgttgatatt actgctacca atgccgctgg gtccgataca
     2221 aagactttgg tattgaccat taatccaact atttccttcg ctgtgtcaag tgataacata
     2281 tcagaaaatg gaggcagtta tgtgcttact cttaatggtt gtggcacggg tgcatacgga
     2341 gtaaatgtta gcgtaacagg aggtactgcc cctttatcgg attatacctt tgtttctcct
     2401 accacagtat cattcagcgg cagcggaatt caagtaatta atatccccat cgtaaataat
     2461 gcagttgtag atggcaacag aaccattacg tttggattaa gcagctttac aggaagtgca
     2521 gtcgtgggca gcaatgccag ctttagcctt acaattgtag atgatgaaat tccggcaccg
     2581 gttataacac atactgtcat tccaaacact acaagtatta cagcgcaggt tggtattgcc
     2641 agtgtgacca attcgccaag tagcgtgata gctacatacc gccgaaatgg tggtggttgg
     2701 ggtactgtaa atgcaacttt tgtaagtggt ataaattaca gttttacaat acccggtatg
     2761 ccacaaggta gtactataga atattatata acagctacca atagtggtgg aacagattta
     2821 aagcctagtc ctactactta ttattcttat acggtaggtt gcacaccacc tgctgccggt
     2881 acaaaaataa tagcttttca aggattcgaa tttccggaaa caggctatac gggtattaca
     2941 gtgcctaatc aaagtgctac accaacagga ctgcaaaact atttgccaca tccgtcgttg
     3001 gtggaaatga attattatct caggggtacc aacggtgttc ccttacccgc caatagaaac
     3061 tcttgtggct gcatggcaga aacttttgta acaggaccac ctccatctac cttttacgat
     3121 aatggagtga atggtgcttt aaattccaac actatttgta gaaactttag tggcagccaa
     3181 cgtggcgttt ataaaaacgg aggaggctca ccaagtacag acaacccagc aatgcctggt
     3241 agcgatggtt ttgcccgaaa tggcaattat gcatatgtgc aacaatcaag aaatgatggc
     3301 ggtgaggggc caatcagtaa aatttatttt gatgccatcc aaatacccga ctttgctaat
     3361 gccaccaacg ttaaaataaa gttgtatgta tcatcgatct caaccaatgg aaatggcgat
     3421 gaagatgatt ttgtaagagc ttttgcaaga tatggcgcaa cagctgcaag tgctactatc
     3481 gttaataatt tggccagttc ttctgattgg tttaatgcaa gtactaatag tctggtatct
     3541 actactgtta ccggaacatt atccaatagc gaacgttggg attatttagg caacatgtac
     3601 aatacaacgg gcactattac aggcactacc accaaaaaca ttattgaact aagcatacct
     3661 gcgggtaatg aatatgttcg cctaatgatt gatattttga atgacgattc aaacaataga
     3721 gaactttggg caattgatga tatacaaata atagcggatt acacaacaac ggcaa
//
LOCUS       DAORQA010000181         3756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5947_length_3756_cov_13.507971, whole genome shotgun sequence.
ACCESSION   DAORQA010000181 DAORQA010000000
VERSION     DAORQA010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5947_length_3756_cov_13.507971"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="PK736_07780"
     CDS             <1..1363
                     /locus_tag="PK736_07780"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRC33328.1"
                     /translation="LLSRATFGIAVNDVADLKNKIPKILLQSLMNDSQNIVPFDSVDN
                     TAEEEMQNKRDAKQDMAAIIQSKRAEILALNLAWMEKLQSDKAQLREKMTFFWHGHFA
                     CRIRFAAMAQSLNNIIRENALGNFGDLLMAVSKSPAMILFLNNQQNRKQQPNENFARE
                     VMELFTMGRGNYTEKDVKEAARAFTGWAFKRRAGEFEFRENQHDDGRKIFLGEEGNFK
                     GEDIIAIILKQKKTAQFIVNKIYKFFVNENIDSSITTKLADDFYKSNYNIEQLMFTIF
                     SADWFYDPINVANRIKSPVELATSMNKLFTPVYDNEKYMLRIQQALGQVLFFPPNVAG
                     WPGGRAWIDSSSLFFRLKLASVILNKGSFDFDEKDNMDDELFMINRKQKADKIAMLKI
                     EVDWQKILDQFSKIKNENELQILLLGKSLSPEQQKLLPEFSTANLKEYILSLLSLPEY
                     QLS"
     gene            1372..2565
                     /locus_tag="PK736_07785"
     CDS             1372..2565
                     /locus_tag="PK736_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRC33329.1"
                     /translation="MNRRDFIKQSALVSGSILLPSFLKPLSALGNSTGNKKLVIIQLS
                     GGNDGLNTIVPYRNDIYYKVRPSISIAKDTVLKLNDELGFNPALKPLKSLFDAGNMCI
                     INGVGYPNPDKSHFRSMDIWQSASSSSEVISTGWLGRYLDSNCDGCIQPHHAIETDDS
                     LSLALKGAARKGMAIKEPAQLSRVANEPFIKNVCSAVQTNMLTDDNLGYLYKTLIESE
                     QSANYVYSKVKTQKNIAEFDKEKFSQSMSLISRFILSGLSTKVYYTSIGSFDTHANQN
                     NQQDRLLGQFAQGVKSFMDDIEQSSFKDDVLIMVFSEFGRRVAQNAGQGTDHGTASNM
                     FLIGHNLKKQGLYNNPSNLQNLIDGDLIYDVDFRDVYATVLNKWLDADAKKIIGNGCN
                     VLDFL"
     gene            2716..3744
                     /gene="ruvB"
                     /locus_tag="PK736_07790"
     CDS             2716..3744
                     /gene="ruvB"
                     /locus_tag="PK736_07790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRC33330.1"
                     /translation="MNKNLDADSENLTPADREIEKALRPLSFGDFTGQFSVVENLQVF
                     VKAATLRGEALDHVLLHGPPGLGKTTLANIISNELKVNIRTTSGPVLDKPGDLAGLLT
                     NLEPNDVLFIDEIHRLSPIVEEYLYSAMEDYKIDIMIESGPNARSVQIKINPFTLIGA
                     TTRSGLLTAPLRARFGINSRLEYYDSKLLQLIVLRSSEILKMEISDEAAFEIARRSRG
                     TPRIANALLRRIRDFAQIIGNGTIDMEIAQYGLKALNVDAHGLDEMDNRILAAIIDKF
                     KGGPVGLTTISTAVGEESGTIEEVYEPFLIQEGYLMRTPRGREATEMAYKHLGRIKNA
                     AQGGLFDS"
BASE COUNT         1249 a          685 c          771 g         1051 t
ORIGIN      
        1 tctgctatcg cgtgcaactt tcggtattgc agtcaatgat gtggcagatt taaaaaataa
       61 aattcccaaa atacttttgc agtccctgat gaacgattct caaaatattg ttccgtttga
      121 cagtgtagac aatactgccg aggaagaaat gcagaataaa agagacgcaa agcaagatat
      181 ggcggccata atacaatcga aaagggcaga aatacttgca ctcaatcttg catggatgga
      241 aaaattgcaa agcgacaaag cacagttacg cgagaaaatg acattctttt ggcacggtca
      301 ttttgcatgt cgaattcgtt ttgctgctat ggcacaatca ttaaataata ttatcagaga
      361 aaatgcgctg ggcaactttg gcgatttgtt aatggcagtt tccaaaagtc cggcaatgat
      421 tttgtttcta aacaatcagc aaaatagaaa gcaacaaccc aacgaaaact ttgcgcgcga
      481 ggtgatggaa ttgtttacaa tgggccgcgg aaactatact gaaaaagatg tgaaagaagc
      541 ggcacgggca tttactggtt gggcatttaa acgcagagca ggagaatttg aattccgcga
      601 aaatcaacat gacgatggca ggaaaatctt tcttggcgaa gaaggtaatt ttaaaggcga
      661 agatattatt gcaataattt tgaagcagaa aaaaaccgca cagtttatag tgaacaaaat
      721 ttataagttt tttgtaaatg aaaatattga cagcagcata accacaaaac ttgctgatga
      781 tttttataaa tcaaactaca acattgagca actgatgttc actatttttt cggccgattg
      841 gttttacgat cccattaatg tggccaaccg aattaaatct ccggttgaac tggcaacatc
      901 gatgaataaa ttatttactc cagtatatga caatgaaaaa tacatgctgc gtatacaaca
      961 agcgttaggg caggtattat tttttcctcc taatgtggca gggtggccgg gaggtcgtgc
     1021 ctggattgat agcagttcct tatttttcag gcttaaactg gcatcggtaa tactaaacaa
     1081 aggctcgttc gattttgacg aaaaagacaa tatggacgat gaactattta tgataaaccg
     1141 aaaacaaaaa gccgacaaaa ttgctatgct gaaaatagaa gtagattggc aaaaaatact
     1201 tgatcaattt tcgaaaatta aaaacgaaaa cgaattgcaa attttattgc tcggaaaaag
     1261 tttatcccct gaacaacaaa aattattgcc cgaattttct actgccaatt tgaaagaata
     1321 tattttatca ttattgtcgt tacccgaata tcaactttca taatacagaa aatgaacaga
     1381 agagatttca taaaacaatc agcactggta tcaggatcaa ttctgctacc atcattccta
     1441 aaacccttgt cggcactggg aaacagcaca ggcaacaaaa aacttgtgat cattcagttg
     1501 tcgggtggca acgatggatt gaatactatt gtaccctatc gtaatgatat atattataaa
     1561 gtgcggccaa gtatttcgat tgcaaaagac acggttttaa agttgaatga tgaactgggt
     1621 tttaatcctg cattgaaacc tttaaaatca ttattcgatg caggcaatat gtgtatcatt
     1681 aatggtgtag ggtatcccaa tcccgacaaa tcacattttc gttcgatgga tatttggcaa
     1741 agtgccagca gcagcagtga ggtaatttct acaggatggc ttggtcgtta tctcgattcg
     1801 aattgtgatg gatgtataca gcctcatcac gctattgaaa cagacgattc gctttcgctt
     1861 gctctgaaag gcgcagctcg caagggaatg gcaataaagg aaccggcaca actgagcaga
     1921 gtggctaacg aaccctttat aaaaaatgtt tgtagcgcag tacaaacaaa tatgctaacc
     1981 gatgataatc tgggatattt gtacaagacc cttattgaaa gtgaacaaag tgccaactac
     2041 gtttattcga aagtaaaaac acaaaagaat attgctgaat ttgataagga gaaattttcg
     2101 caaagcatga gtcttatttc acgcttcatt ctttcgggat tgtctacaaa agtttactac
     2161 acttctattg gtagtttcga tacacatgca aaccaaaaca atcaacaaga ccgattgctt
     2221 ggtcaatttg cacaaggtgt aaaatctttt atggacgaca ttgaacaatc gtcatttaaa
     2281 gatgatgtgc tcataatggt gttctcagaa tttggtcgta gagtagcgca aaatgccgga
     2341 caaggaactg atcacgggac ggccagcaac atgtttttaa tagggcacaa tttaaaaaag
     2401 caagggctat acaacaaccc atcgaaccta cagaatttaa ttgatggcga tttaatatac
     2461 gatgtagatt tcagagatgt ttatgccacg gtattaaata aatggctcga tgccgatgca
     2521 aaaaaaataa ttggcaacgg atgtaatgtg ctcgactttt tatgatttat ttcacacaaa
     2581 ataatttcat tcgaatatca attgctaaaa atttctgttt ggcaagcatg ttgaattttt
     2641 cgtttcccaa aatatacaag tttgttatac attgcgtttt gtgtttttat gtaattggca
     2701 taacttcgca cgatgataaa taaaaacctt gatgccgata gtgaaaatct aaccccggcc
     2761 gatcgcgaaa tcgaaaaagc attgcggcca ttatcatttg gcgattttac cggacagttt
     2821 agtgtagtag aaaatttgca agtatttgta aaggctgcaa cattgcgcgg tgaagcttta
     2881 gatcatgttt tactgcatgg ccctccgggt ttaggaaaaa ccactttagc caatattatt
     2941 tcgaacgaac tgaaagtaaa tattcgcact acatcgggtc cggtgctcga taagccgggc
     3001 gatcttgctg gactgctcac taatctcgaa cccaacgatg ttttgtttat tgatgaaata
     3061 catcgcttgt cacccattgt cgaagaatat ctttactctg caatggaaga ttacaagata
     3121 gatattatga tagaaagcgg ccccaatgct cgcagtgttc agataaaaat aaatcctttt
     3181 acactcatag gtgctaccac tcgttcggga ttattaacgg cacctttgcg cgcacggttc
     3241 ggcataaatt cgcgactgga atattacgat tcgaaattat tacaacttat agttttgcga
     3301 agctccgaaa tattgaaaat ggaaataagc gatgaagcag cttttgaaat tgcccgcaga
     3361 agccgaggca caccacgtat tgcaaatgca ttgctcagac gcattcgcga ttttgcgcag
     3421 attattggca acggaactat cgatatggaa atagctcaat acggattgaa agcacttaat
     3481 gtagatgcac acgggttgga cgaaatggac aacagaattc ttgcagcaat catcgataaa
     3541 tttaaaggtg gtccggtagg tctcacaaca atttcgactg cagtgggcga agaaagcggc
     3601 accatcgaag aagtgtacga acctttttta attcaggagg gttatctcat gcgcacacca
     3661 cgcggtcgcg aagccaccga aatggcttac aaacatcttg gcagaataaa aaatgctgcg
     3721 caaggtgggt tgtttgattc ttaaattctt gtcatt
//
LOCUS       DAORQA010000182         3737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_5998_length_3737_cov_27.197719, whole genome shotgun sequence.
ACCESSION   DAORQA010000182 DAORQA010000000
VERSION     DAORQA010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5998_length_3737_cov_27.197719"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(313..2067)
                     /locus_tag="PK736_07795"
     CDS             complement(313..2067)
                     /locus_tag="PK736_07795"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HRC33331.1"
                     /translation="MKKQNLILAIMCIIAMPLISNAQAFKYDLIHHFGNAGCGICATQ
                     NPVFKSTILNPNTGKIHHIKVPTSFPYFDPMYAAIPVLCDSLTNYYKITSVPTAVQNG
                     NFKKSVLSSVTQSDVNTINLQSSPVTVLVSHTTSGQTRTVNVEIKNVSTATLATGYVL
                     QLAVVERVITYLVAPGGNGEKVFPDVLRAQFPANNGYTGQVITLPAPGMTSIYTFTYT
                     LNNTWVASQIEEIAYVQNTASKEVMNSGASYDCQINSVLSSTSIAICQGSTATIQTNA
                     GNGNTYVWKKSGVVIPNAISANYSTTVSGDYTVEISNGSCTAYSTKSKVTVNPIPSAV
                     TLSQTGIVNICSGKSLNVTVTNPIGTNSYSWLKNNITIPLATATNYTITTTGKYKARA
                     TNEFGCVSTSALVDAKVTSASSVNVIPAGPLTMCAGDSMLLTASNNANHTYQWKRNNV
                     NVSNATALNYYARQSGNYKVIVTNINGCTKVSNTVAITINCREANAFNNIEQGISLYP
                     NPADNRVTIEIGKLDISTFKVLNSTGQIMQLEILPVDFENGTYELNTAEMKSGFYIIQ
                     CNDQNGKTIVSKFQVIHE"
     gene            2199..3455
                     /locus_tag="PK736_07800"
     CDS             2199..3455
                     /locus_tag="PK736_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33332.1"
                     /translation="MKKYFSAFILFVFVISGMCAQSIKDETIVYNYIKLPSNPLDRNI
                     KNYQASIVANYANDNAEKKTQYDAEYKIALADFEKDKKEYPAKVKAAEDSYVKEMEEW
                     NKKSLGEKLIEKQVLNQNNKPVKQLPPVPSMRYVAEPDLKTTYDFPALCNTYFNLEGF
                     NNSPDNAINIVITFYGYDYTQPHQLSNTKDMTRYANGQTTNYKATYYYTEFTYRHAMS
                     VKVSDASGHEILNVTPQELNNYKTYKSTESEIPPQINSQQLVKSFEEKILQENLKFIN
                     NLLNDQFGYKRTERKTELYYVKSKGDEYKDLLVAFNEASSGLKTLIDDAQPARTKITS
                     ALQAWNAALQQSDVKDKKARIDKDVTIALCFNLLEAFFAMGNAENADKIFTMLNGIEI
                     SNGDRKIKESYEALFNDFKKRVKSNN"
BASE COUNT         1226 a          613 c          672 g         1226 t
ORIGIN      
        1 tataaatttc ggtagttgct ttctttttta tctattctaa aaaaatatct taaaaaaaac
       61 tacgacagat ttttgtgtgc caattgcaat ttcttttttc tctaaactca aacttctttg
      121 gtataaactt aattgttttt atttcgatgc caatcagtaa caaagaaaaa tgacattttg
      181 aaaagtgaac gattaacgtt gatagcgttt attgaaatgc aatggttttc acacatttta
      241 aatttttttt cattgcaaaa aaaaggtgca aaatgattgc acctttttaa aaatattgct
      301 gagagaaatt gcttattcgt gtattacttg aaatttggaa acaattgttt ttccattttg
      361 gtcattacat tgaataatgt aaaagccgct tttcatttct gcagtgttta attcgtatgt
      421 accattttca aaatctactg gcaatatttc cagttgcatt atttgtcctg tactatttaa
      481 aactttgaaa gtggaaatat ccaatttgcc aatttcgatt gtaactcgat tatcagcagg
      541 attggggtaa agtgatatgc cttgttcaat attattaaaa gcatttgcct cgcggcagtt
      601 aatagttata gcgacagtat ttgatacttt ggtgcatcca ttaatgtttg ttactattac
      661 tttatagttg ccactttgcc tggcataata atttagtgcc gttgcatttg aaacatttac
      721 attgtttcgt ttccactgat atgtatggtt tgcattatta ctggcagtaa gcaacatact
      781 atcacctgcg cacattgtaa gcggcccggc cggaatgaca ttcacactgc ttgcacttgt
      841 aactttggca tcaaccaaag ctgaagtgct tacacaacca aactcgtttg tggcacgtgc
      901 tttatattta ccagtggtgg tgatggtata atttgttgct gtggcaagtg gaatagtgat
      961 gttgtttttt aaccatgagt atgaatttgt tcctatagga tttgtaacgg ttacatttaa
     1021 actcttgccg ctgcaaatat ttacaatgcc ggtttgcgaa agtgtaactg cagatggtat
     1081 gggattaaca gtaacttttg attttgttga ataagccgta caagaaccat tcgatatttc
     1141 cacggtgtaa tcacctgaaa cagtagtact gtaatttgca ctaattgcat taggaataac
     1201 cactccgctt tttttccaaa cataggtatt tccatttccg gcattggttt gtattgttgc
     1261 agtgcttcct tggcaaatgg caattgatgt tgaagataaa acagaattta tttgacagtc
     1321 gtacgatgca ccactgttca ttacttcctt gcttgcagta ttttgcacat aagcaatctc
     1381 ttctatttgc gatgctaccc atgtattatt aagtgtgtaa gtaaatgtgt atatgctggt
     1441 catacctggt gcaggcaacg ttattacctg ccccgtgtat ccattgttgg ccggaaactg
     1501 tgcacgcaac acatcaggaa aaaccttttc gccattacct ccgggagcaa ctaaatatgt
     1561 tattactctt tctactactg caagttgtaa tacatagcct gttgccaaag tagcagtaga
     1621 tacatttttg atttctacat ttactgtacg cgtttggcca cttgtggtat gactcaccaa
     1681 aacagtaaca ggactgcttt gcaaattaat agtgttcaca tcgctttgag taaccgatga
     1741 taaaaccgat tttttgaaat tgccattttg caccgcagtg ggtacactcg taattttata
     1801 atagttggtg aggctatcgc aaagtacagg aattgcggca tacattggat cgaaatatgg
     1861 aaaacttgta ggtactttaa tatggtgtat tttacctgta ttgggattta aaattgttga
     1921 tttaaatacc ggattttggg tggcgcaaat tccacaaccg gcatttccaa aatgatgtat
     1981 caggtcatac ttaaacgctt gtgcattcga tattaaaggc attgctatga tgcacatgat
     2041 agcaagaatt aaattttgtt ttttcatatc cagataattt ttaatgattt ttaatgatgt
     2101 aaagaaaaca tttttaggga tattgaactg tcgtacagca gacatcttaa caaaaggaaa
     2161 cctttttttt atacattcgc gtcctcaaaa aaatcaacat gaaaaaatat ttttccgcat
     2221 ttatattgtt tgtatttgta atttcaggca tgtgtgcaca atctataaaa gatgaaacca
     2281 ttgtatataa ttatataaaa ttgccttcaa atccgttgga tagaaacata aaaaactatc
     2341 aggcaagtat agtagcgaat tatgcaaatg ataatgccga aaaaaaaaca cagtacgatg
     2401 cagaatataa aatagcactt gccgatttcg agaaggataa aaaggaatac cctgcaaaag
     2461 tgaaagctgc cgaggatagt tatgtcaaag aaatggagga atggaataag aaatcgcttg
     2521 gcgaaaaatt gatagagaaa caagtgctta atcaaaataa taaacctgta aaacaactgc
     2581 caccagtacc ttctatgcgt tatgtggctg agcccgattt gaaaaccaca tatgattttc
     2641 cggcattgtg caatacatat tttaatttgg aaggtttcaa taatagtcct gataatgcaa
     2701 ttaatattgt gattactttt tatggttatg attatacaca gccacaccaa ctatcgaaca
     2761 caaaagatat gacacgctat gccaatggac aaacaacaaa ctacaaagcc acttattatt
     2821 atacagagtt tacataccgg catgccatgt cagtaaaagt aagtgatgca tcgggccacg
     2881 aaattctgaa tgttactcca caagaattga ataattataa aacctataaa agcacagaat
     2941 ccgaaattcc gccacaaata aattcgcagc aactggttaa gtcttttgaa gaaaaaatat
     3001 tgcaggaaaa tttgaaattc atcaataacc tcctcaacga tcaatttgga tataaacgca
     3061 cagagcgcaa aacggaattg tattatgtaa aatcgaaagg tgatgaatac aaagatttat
     3121 tagtggcttt taacgaggct tcatcgggct tgaaaacact catagatgat gcacaacccg
     3181 cacgtacaaa aataacaagt gctttacaag catggaatgc tgcactgcaa caatcggatg
     3241 tgaaagataa aaaagcccga atagataaag atgtaacaat agccttatgc ttcaatttgc
     3301 ttgaagcttt ttttgctatg ggcaatgcag agaatgccga caaaatattc actatgttaa
     3361 atggaattga aatatcaaac ggtgatagaa aaataaaaga aagttacgaa gcgttgttca
     3421 acgattttaa aaaacgcgtt aagtcgaata actagttttt ggttattctg ttacatggtt
     3481 attaggttat tggttattct gttactggtt attctgttat tagttattaa gttatttggt
     3541 tattggttat taatgcgaat caaaggttga aaaaattcaa tgccagcaag gctgaaagca
     3601 atgtaaaatt caaagtaaaa aagttaaacg aaaatgtttc ttagtaaaac ttagtgcctt
     3661 cgtgccattg tggccaataa gcattttagt ttcaaaacgt tactcttggc ttgcattatt
     3721 agtttatagt tataatc
//
LOCUS       DAORQA010000183         3667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6119_length_3667_cov_7.286545, whole genome shotgun sequence.
ACCESSION   DAORQA010000183 DAORQA010000000
VERSION     DAORQA010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6119_length_3667_cov_7.286545"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1040)
                     /locus_tag="PK736_07805"
     CDS             complement(99..1040)
                     /locus_tag="PK736_07805"
                     /inference="COORDINATES: protein motif:HMM:NF015532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family outer membrane porin"
                     /protein_id="HRC33333.1"
                     /translation="MLNTPFINLQDGRMRPSEVEGAYFEINEIKKTKIEGGWLYGFSP
                     RSTTKWFRGASSIGVYSSGVNTDGTKSQYANNLTSNGVFMLGIHNEAIKNFKLHAWDV
                     FIENILNTAMLQADWQLPLKKESSIFSSAQVIRQDAIKYGGNKNQSKTYVDKEAKAMA
                     FGVRAGWKNKKIETSINYNRITADGRYLMPREWGRDPFFTFLPRERNEGFGDVNAIMA
                     KVNYNFDKVRIKTSLAGGYYNLPDVKNYALNKYGLPSYTQINADVRYSFAGVLKGLEA
                     QILVAGKINDGNIYNNDKYVINKVDMVNYNFVLNYNF"
     gene            complement(962..1516)
                     /locus_tag="PK736_07810"
     CDS             complement(962..1516)
                     /locus_tag="PK736_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33334.1"
                     /translation="MMKKIITAAVMLLTGNTIYAQHQEVNEKPATWNSKNEVVTDSTS
                     LLHAFKAGKFNGSFRYFLMATDNVTGLTDYYANAAGGGLRYETSKFYGLQFALSGYYI
                     FNIGSSDLGVPESATGQYNRYEIGLFDIQDPYNKKDINRLEELYLKYKIHPSRSVASC
                     LIRPLSIYRMAVCGLQKLKVLTLK"
     gene            complement(1539..1961)
                     /locus_tag="PK736_07815"
     CDS             complement(1539..1961)
                     /locus_tag="PK736_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRC33335.1"
                     /translation="MKKILLLVMAFAFANTITAQTKTNNNKHKIVFQLASDDTLVHKA
                     LMKQLNNIVTVAPETKIEVVVQGGGLTMLMINKTIVQEKIAQLKKKNVEFVACEFSMT
                     DKKITKEMMIPEAGFVPYGILEIVTKQEEGWSYIKSGF"
     gene            complement(1973..3664)
                     /locus_tag="PK736_07820"
     CDS             complement(1973..3664)
                     /locus_tag="PK736_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HRC33336.1"
                     /translation="MKNNRRDFIKKVGIIGGAGLLASTPIASLSKPDDATEHDETLAD
                     DKPFTLSILQTTDVHCQVHPHDELFWENEKTVFRKTGGYAHLDTYFKQERKKNQNTFI
                     IDTGDMFQGSELSVKTTGKAMVPILNELGYDLYLPGNWEVIYYKKAMQTLLGSLNAPK
                     VCANMYHDLGEGKKGELIFPPYYIWNIKGVKIGFLGYTDPLVPLRQSPNYSKGIIYTK
                     PEENLAHYVDVLRNQEQCASVLIVAHLGLSQQIALANLEECNGVDYILGGDTHERVRK
                     PIVCKYSKVVEPGAFGSFVGKLNLTVQKGKVIKDEYELVEITADKFKPSKQVSALIAE
                     NQKPFDQDINTIAGYSTIPLFRYFVVENPIDTMILDALKWKMTEVDIVLSNGFRFCPP
                     RTTPDHTGNIPITDGYIYDMFPVDSTVRTGTVTGRQIQEWLEKELNNVFAKNASERFG
                     GWVIKFKGMKVKFDAFGEKGKRVHEIIVGSTPLDPNKTYNICACERDGDPSDMLCRMK
                     NVSNAKNTTVTLHSVMKDYLAANSPVTPAPPLNADVLDAPQTLLTQVTGVDYQFR"
BASE COUNT         1084 a          733 c          608 g         1242 t
ORIGIN      
        1 aaaatataca atatggagaa tactgtattt ccttttaaaa tccagtacta ctttgaaaag
       61 aaatagttta aattaacgaa aagaaaattg ataatatttt aaaaattata attcaataca
      121 aaattgtaat tcaccatgtc cactttgtta atcacatatt tatcgttgtt ataaatgtta
      181 ccatcattaa tttttccggc taccagtatt tgcgcttcaa gtccctttaa aactcctgca
      241 aaagaatacc tcacatctgc attaatttgc gtgtaagaag gcaagccata tttattaagc
      301 gcatagtttt ttacatccgg caaattgtaa tacccgccag caagcgaagt tttaattctt
      361 actttatcga aattgtaatt cacttttgcc atgattgcat ttacatcacc aaaaccttca
      421 ttacgttcgc gaggtaagaa ggtaaaaaac gggtcgcgtc cccactcgcg cggcataagg
      481 taacgaccat cagcagttat gcgattgtag ttaatactgg tttctatttt cttgtttttc
      541 cagccagcac gcacaccaaa agccattgcc ttcgcttctt tatcaacata cgttttcgac
      601 tgatttttat ttcctccata tttgattgca tcctgacgaa tgacttgtgc tgacgagaat
      661 attgaacttt cctttttaag tggaagctgc caatcggcct gaagcattgc tgtgttcaaa
      721 atattttcga taaaaacatc ccatgcatgc aacttgaaat tttttattgc ttcgttgtga
      781 atgcccagca taaaaactcc gttagaggta aggttattgg cgtattgcga tttggttcca
      841 tcagtattta ctcctgaaga ataaacacca attgaggatg cgcctctaaa ccatttggta
      901 gtgctgcgtg gtgaaaaacc atacagccag ccaccttcaa tttttgtttt tttaatctca
      961 tttatttcaa agtaagcacc ttcaacttct gaaggccgca tacggccatc ctgtaaattg
     1021 ataaagggcg tattaagcaa ctggcgaccg aacgagaggg atgaattttg tatttgagat
     1081 ataattcctc gaggcggtta atatcctttt tattgtaagg atcctgaata tcaaacaaac
     1141 caatttcata acggttgtat tgaccagtgg cactttcagg cacacctaaa tcagaagatc
     1201 cgatattaaa tatataatag ccgctcaaag caaactgaag gccataaaat tttgatgttt
     1261 catagcgtaa tccgccaccc gcagcatttg cataataatc agtaagacct gttacattat
     1321 cagtagccat taaaaaatac ctgaagctgc cgttgaattt tcctgctttg aaagcatgca
     1381 ataacgaagt agaatctgtt actacttcat tttttgaatt ccaggtagct ggtttttcat
     1441 ttacttcctg gtgttgtgca tatatagtat ttccggtaag cagcattact gctgcagtta
     1501 tgattttttt catcatatat tttaaaagtg attagtaact aaaagcctga tttgatatag
     1561 ctccagcctt cttcctgctt agttacaatt tcgaggatgc catagggaac aaaacccgct
     1621 tcgggtatca tcatttcttt ggttatcttt ttgtcagtca ttgaaaattc gcaggcaaca
     1681 aattcaacat ttttcttttt aagctgcgca attttttcct gaacaattgt tttgttaatc
     1741 atcagcatgg ttaaaccacc gccctgaaca acaacttcaa tttttgtttc aggggcaaca
     1801 gttacaatat tgtttaattg tttcatcagt gctttgtgca ccaacgtgtc atcagatgcc
     1861 aattgaaaaa caattttatg tttattgtta tttgtttttg tttgcgctgt tatggtattg
     1921 gcaaaagcga aagccataac taataagagg atttttttca tagtatttgt ttttagcgaa
     1981 attgataatc tactcctgta acttgcgtta gcaatgtttg cggtgcatcc aacacatctg
     2041 cgtttagcgg aggtgctggt gtaacaggtg aattagctgc aaggtaatcc ttcatcactg
     2101 aatgtaaagt aacagttgtg ttttttgcat tggaaacatt tttcattctg caaagcatat
     2161 cacttgggtc tccgtctctt tcgcaggcac agatattata tgttttatta ggatcaagag
     2221 gagtgcttcc tacaattatt tcatgtactc gttttccctt ttctccaaaa gcatcaaatt
     2281 ttactttcat tcctttaaac tttataaccc agccaccaaa tcgttcggat gcattttttg
     2341 caaatacatt attcaattct ttttccagcc attcctgaat ttgcctgccc gttacagttc
     2401 ccgttctcac tgtactgtca accggaaaca tatcataaat atacccgtca gtaattggaa
     2461 tattgcctgt atgatcaggt gttgttcgcg gaggacaaaa gcgaaaaccg tttgataata
     2521 cgatatcaac ctcggtcatt ttccatttca aagcatccag tatcatggta tcaataggat
     2581 tttcaacaac aaaatatcta aacaatggaa tggtactgta acctgcaatt gtatttatat
     2641 cctgatcaaa tggtttttga ttttcggcaa tcaatgctga aacttgtttt gaaggtttga
     2701 atttatcagc tgtaatttct accaactcat attcatcttt aatcactttg cctttttgca
     2761 cagtaaggtt aagtttacca acaaaagagc caaatgcacc tggctcaaca actttgctgt
     2821 atttacaaac aattggtttg cgcacacgct catgcgtatc gccaccaagt atgtaatcaa
     2881 caccattaca ctcttccaag ttagccaatg caatttgctg cgacaatcct aagtgtgcaa
     2941 caattaatac agaagcacat tgttcctggt tacgcaacac atccacataa tgtgcaagat
     3001 tttcttcggg cttcgtataa ataatcccct tactgtaatt aggcgattga cgcaatggaa
     3061 caagcggatc ggtataacct aaaaatccga ttttaacgcc ttttatattc catatataat
     3121 atggaggaaa aatgagttcg cctttttttc cttcgcctaa atcgtgatac atattggcgc
     3181 aaacttttgg tgcatttaat gagcccaata aagtctgcat tgctttctta taatagataa
     3241 cttcccagtt tccgggcagg tataaatcat aacccaactc atttaaaata ggaaccattg
     3301 ctttgcctgt agtttttact gacaactcgc tgccctgaaa catatcaccg gtatcaatga
     3361 taaaagtatt ctgatttttt tttcgctcct gtttaaagta ggtatcaaga tgagcgtatc
     3421 cacctgtttt acgaaacaca gttttttcat tctcccaaaa caattcatca tgtggatgta
     3481 cctggcaatg cacatctgtt gtttgtaaaa tagaaagtgt aaaaggttta tcatccgcta
     3541 aagtttcatc atgttcagtc gcatcatctg gttttgacaa agatgcaata ggcgttgatg
     3601 ctaataatcc tgcaccacct attatgccaa cttttttaat gaaatctctt ctgttatttt
     3661 tcatgaa
//
LOCUS       DAORQA010000184         3628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6200_length_3628_cov_9.824797, whole genome shotgun sequence.
ACCESSION   DAORQA010000184 DAORQA010000000
VERSION     DAORQA010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6200_length_3628_cov_9.824797"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PK736_07825"
     CDS             <1..303
                     /locus_tag="PK736_07825"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRC33337.1"
                     /translation="TLGYIDLFDIDYFSKRAGISILIGEVENRGKGFASEALTAIINL
                     AKEDLKLFQLFCNIHVTNIASMKLFTSHGFEQTGILKKWSFDGNEFVDVIVMQLFL"
     gene            337..2328
                     /locus_tag="PK736_07830"
     CDS             337..2328
                     /locus_tag="PK736_07830"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC33338.1"
                     /translation="MKKLLTYTVVVTVVIANLFCNYTTTPALERTEFVSLTDSIHYAG
                     MNSCKECHQSIYDSYIQTGMGKSFEKASKKKSSADFSNSKIYDKFKDLYYQSFWNGDS
                     LQFMEYRLYKGDTIHKRIETVHYIVGSGQHTNSHMVVTNGYFNQAPMTFYTQKKKWDF
                     PPGFEDGHNTRFNRLIGLECMTCHNSYPEFVEGSVNKFESVPEGISCERCHGPGSAHV
                     AEKQAGKLVDISQKIDYSIVNPAKLSIEKQFDICQRCHVQGNAVLRKGKSFYDFLPGM
                     KLSDVMNVFMPVYSNSSEHIMASHAERLKLSQCYLQTTQKISADKNLSNQLRPYKNAL
                     TCVTCHNPHISVKSTGIDHFNNTCKNCHSEGKSMKCSADKKLLESKQNNCVSCHMPKS
                     GATDIPHVSVTEHFIRKPISEKEFSAIKNFVTISCINNPNVEQLERGRAYISYCEKFE
                     LGQMALDSAKKIFNDQTPDEIKQNFEELIHIAYLEKNAGNIIRYLNAVPDAIKTLNKK
                     SFENTDAWTCYRIAQMIEVNQPGRAEVFYKRCIELAPYHIDFLNKYGNILVTLNKQNE
                     AKDVFAKAVSLYPKNAQSLSNLGYLTLLTEGDAEKAISFYNRAIALDPDYEKAFINKA
                     GALVYQNKINDAKAVLFFFLLHHKDAVESKEVMKRLKDA"
     gene            2426..3490
                     /locus_tag="PK736_07835"
     CDS             2426..3490
                     /locus_tag="PK736_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33339.1"
                     /translation="MLFVSAKGKDDPIPVLIRASAANVVGIEEGDFVSLFNDVVYNEI
                     INNRVKLWDSFSKEIQLSGVTLQEIERNSSALFKEQKYIYVFENWKRTSKEIQSTTLG
                     FLFTTKSKTGEDVSFGYVEYNELSAALQKVKLGVSADGYYNQTLALLLNTKKFAFDII
                     QFGGEVIKSSEDNDRIKAEFIGKLGFNVVLFNASEPQKMITYTIERSVQLNDELAENS
                     TVFLDAIELYLINHPDKIAEFGNEELANKSIVDLKVNRVEIKELWVRNGDKLVYSPQQ
                     LVIYLNETPMKPMSNTRLQALSISFQGTDVFTYLKKKNFNFIINAINGQRISRSQSFF
                     YLRALQEGDWRAINEYVKNM"
     gene            3554..3627
                     /locus_tag="PK736_07840"
     tRNA            3554..3627
                     /locus_tag="PK736_07840"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3588..3590,aa:Ala,seq:ggc)
BASE COUNT         1198 a          627 c          739 g         1064 t
ORIGIN      
        1 actttggggt acatagattt attcgatatt gattatttta gtaaacgtgc aggtatttcc
       61 attttgatag gtgaagttga aaacaggggc aaaggctttg cttcggaggc gcttactgca
      121 attattaatc ttgccaagga ggatttgaaa ttgtttcaac tattttgcaa catacacgtt
      181 acaaacattg ccagcatgaa actgtttacc agccatggct ttgaacaaac cggcattctt
      241 aaaaaatgga gcttcgatgg aaatgagttt gtggatgtaa tcgttatgca attgtttttg
      301 taagatgata aatttcgttt ttactttttg tgattgatga aaaaattgct gacgtatacg
      361 gtggtagtta ctgtggttat tgcaaattta ttttgtaatt atacaaccac acctgctctt
      421 gagcgcaccg aatttgtttc attaaccgat agtatacatt atgccggaat gaattcgtgc
      481 aaagagtgcc atcaatcgat ttatgattcc tatatacaaa ccggaatggg taagtcgttc
      541 gaaaaagctt cgaagaaaaa aagcagtgcc gatttttcga attccaaaat ttacgataag
      601 tttaaagatt tgtactacca atcattttgg aatggcgata gcctgcagtt catggagtac
      661 cgattgtata aaggcgatac cattcataag cggatagaaa ctgttcatta tatagtagga
      721 tcggggcagc acacaaactc gcatatggtt gttaccaatg gctatttcaa tcaggcgcca
      781 atgacatttt atacgcagaa aaaaaaatgg gattttccac cggggtttga agatggtcac
      841 aatacgcgct tcaacaggct tataggtctt gagtgcatga cgtgtcacaa ttcatatcct
      901 gagtttgtgg aagggtcggt aaataaattc gaatcggtac ccgaaggtat ttcttgcgaa
      961 cggtgccatg gacccggaag tgcacatgtt gccgaaaagc aagccggcaa gctggtcgat
     1021 atttcgcaaa aaatagatta ctccattgtt aatcctgcaa agttatctat cgaaaagcaa
     1081 tttgatattt gccaacgttg ccacgtgcaa ggcaatgctg ttttaagaaa gggaaaatcg
     1141 ttttacgatt ttttgccagg catgaaactc agcgatgtga tgaatgtatt tatgcccgtg
     1201 tattcaaatt cgagcgaaca tattatggca tcgcatgccg agcgattgaa acttagccag
     1261 tgctatttgc aaacaacaca aaaaatatca gccgataaaa accttagcaa tcaattgagg
     1321 ccatacaaaa atgccctcac ttgtgtaaca tgtcacaatc cgcacataag tgtgaaaagt
     1381 accggcatcg accatttcaa taatacatgc aagaactgcc acagcgaagg aaagagcatg
     1441 aagtgtagtg ctgataaaaa attgctggaa tcaaaacaaa ataattgtgt ttcgtgccat
     1501 atgccaaaat cgggcgcaac agatatacca catgtgagtg taaccgaaca ttttattcgt
     1561 aagcctattt ccgaaaagga atttagcgcc ataaagaact tcgttaccat ttcatgcatc
     1621 aacaatccca atgtagagca gcttgaacgg ggtcgtgcat atatatctta ttgcgaaaaa
     1681 tttgaacttg gacagatggc attggactca gcaaaaaaaa tatttaatga ccaaacacca
     1741 gatgaaataa aacaaaattt cgaagaatta attcatattg cctatcttga aaaaaatgcc
     1801 ggcaacatta ttcgatattt aaatgctgtg cccgatgcta taaaaacact caataaaaag
     1861 tcatttgaaa ataccgatgc atggacttgc taccgtatag cacagatgat agaagtaaac
     1921 caacccggcc gtgcagaagt gttttacaaa cgttgcattg aattggctcc ttaccatatt
     1981 gattttttaa ataagtatgg aaatatttta gtgacattga ataagcaaaa cgaagcaaag
     2041 gatgtttttg caaaagctgt ttcattgtat cccaagaatg cacaatcgct aagcaatttg
     2101 gggtatctca ctttgcttac agaaggtgat gcagaaaagg caatttcatt ttataaccgg
     2161 gcaattgccc tcgaccccga ctatgaaaag gcatttataa ataaagcagg ggcattggtt
     2221 tatcaaaata agataaatga tgccaaagcg gttttatttt tctttttgtt gcaccacaag
     2281 gatgcagtag aatcaaaaga ggtgatgaaa agattaaagg atgcataata cttcgagatt
     2341 ttgtgttgca tattttctca gacttgttat ttttgccagt aaatttttat ttcattcacg
     2401 ttgagaaaat tactttttat aattcttgct ttttgtttct gctaaaggta aagacgatcc
     2461 cattcctgta ttgataagag cttcggcagc taatgtggtg ggcatagaag aaggtgactt
     2521 tgtaagcctt ttcaacgatg tagtgtacaa cgagataatt aataatcgtg taaaactgtg
     2581 ggattctttt agtaaggaaa ttcagctatc gggcgttacc ctacaggaaa tagaacgcaa
     2641 ctcatcggca ctttttaaag agcaaaaata tatttacgta ttcgaaaact ggaagcgtac
     2701 cagcaaagaa atacaatcga ccactttagg ttttctattt actacaaaaa gcaaaacggg
     2761 tgaagatgtt tcgtttggat atgttgagta caatgagttg agtgctgcat tgcaaaaggt
     2821 gaaattgggc gtaagtgccg atgggtatta caatcaaaca cttgcattgc tgctcaatac
     2881 aaaaaagttt gccttcgata ttattcagtt tggtggtgag gtaattaaat caagtgaaga
     2941 taacgacagg ataaaagctg aatttattgg caagttaggc tttaatgttg ttttgtttaa
     3001 tgcttccgag ccacaaaaaa tgataaccta taccattgag cgtagtgtac agctaaacga
     3061 tgaactggcc gaaaacagca cggtctttct tgatgccatc gaactatatt taattaacca
     3121 tcctgataaa atagcggaat ttggaaacga agaactggcc aataaaagca ttgttgattt
     3181 aaaagttaat cgcgtagaaa ttaaagaact atgggtgcgc aatggcgata aattagttta
     3241 ctcaccacag caacttgtta tttacttaaa cgaaacaccc atgaagccaa tgtctaacac
     3301 gcggttgcaa gccttaagta ttagttttca gggtacggat gtttttacct atctgaaaaa
     3361 aaagaatttc aatttcatta tcaatgctat aaacgggcag cgtatatcgc ggtcacaatc
     3421 ttttttttat ttgcgtgcgc tgcaagaagg cgattggcgc gcgataaatg aatacgtaaa
     3481 aaatatgtaa cctttctttt aatcaataag atttatttct ttgcaccgcc aaaaaaagaa
     3541 aaccaaaaat aatggggatg tagctcagtt ggctagagcg cttgcatggc atgcaagagg
     3601 tcgtgggttc gagtcccatc ttctccac
//
LOCUS       DAORQA010000185         3617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6218_length_3617_cov_4.727120, whole genome shotgun sequence.
ACCESSION   DAORQA010000185 DAORQA010000000
VERSION     DAORQA010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6218_length_3617_cov_4.727120"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PK736_07845"
     CDS             complement(<1..596)
                     /locus_tag="PK736_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HRC33340.1"
                     /translation="MKYLLEKIKLLYERSGIKTRYSVLPDFSCDKDERKFMPKDAKSQ
                     PWPEVQDRMDYYNKSAIGLAKESLMRSLASNVSKNQITHLITVSCTGMSAPGIDIELI
                     EALDLNSNIDRTSVNFMGCYAAIHGLKQADYICRADKDAVVALVCVEICTLHFQNSMD
                     KDHQTANMIFADGAASCLIIGDDVNAGNSRALHINGFYS"
     gene            1004..1954
                     /locus_tag="PK736_07850"
     CDS             1004..1954
                     /locus_tag="PK736_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33341.1"
                     /translation="MGKYLFAVLLLACSFVLHAQINLVPNPSFEQYVSCPNNTGQVDS
                     CVGWYSVLETPDYYNTCAPYPVNIPNNVCGYQNSFDGFGYMGLHTFTWIPVNYREIIG
                     AALIDTIVVGNTYNISMRVSRGNWTIQAYNCSASNKLGILFTTNPITKLTISDINNFA
                     SVYTDSIITDTLNWVMLSWSFVADSAYTGLYIGNFFDDNNTDTLVIAAPLGQFGYAYY
                     YIDSINVYCTSGNCNVGVKDITTNHISVFYNNTSNELNVIFENNSEFKVSIINTLGQI
                     CKTVSISITQKFTLPKLSQGIYYAVLQSAKNTVVKKFTIH"
     gene            1967..2908
                     /locus_tag="PK736_07855"
     CDS             1967..2908
                     /locus_tag="PK736_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33342.1"
                     /translation="MKKIIGFIILAALGAITLNAQTPWYLIGNAPVIGNFLGTTNAQS
                     LQLKTEASQPINLYTSAGVGTFNTGRLTIKGSYVGTSGNGLIGIGDMGTNCAFLPQNL
                     LHQHTTSITRTNFHQFTNPATGSGANNGFLFGQPANSLTGEIRQQEDAPIIIYTGNLN
                     AAQMLERMRFTTGLGGDGVGNWNTISNATKINISSGGIATVPFNFPVACLNIGYDLPG
                     NASGGRRPWMDIGTYYSSNSDHMYTGLLDMGSNRKDAIVNFGDDPQYNAFDNSVQNPE
                     NIRGLIYFNTISIHKKLHTTIISRRAITTRHRYIIQT"
     gene            complement(2843..3343)
                     /locus_tag="PK736_07860"
     CDS             complement(2843..3343)
                     /locus_tag="PK736_07860"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRC33343.1"
                     /translation="MTIAEIFLPDYKLELATTRKILASVDMKLAEFKPHEKSMTMGRL
                     ATHVAEIPGWMNICLVKHELDFASGDFKPVILTTTEELLEKFDKGSAEAIEILSKATD
                     DMFAENWTMRSGDQIFFTLPKLQVVRTWVMNHHVHHRAQLGVYLRLNNIPVPSSYGPS
                     ADDSGM"
BASE COUNT         1125 a          667 c          624 g         1201 t
ORIGIN      
        1 gaataaaatc catttatatg caatgcacgg gaatttcccg cattgacatc atccccgata
       61 atcaggcatg atgctgctcc atcggcaaat atcatatttg cagtttgatg atccttgtcc
      121 atggaattct gaaaatgaag ggtacatatt tctacacaca ccagggctac aactgcatct
      181 ttatcagccc tacatatata gtcagcttgt ttcaatccat gtatcgctgc ataacatccc
      241 ataaaattga ctgaggtacg atcaatattg ctgttcagat ccagcgcctc gataagttca
      301 atatcaatac cgggggcgga catgccggtg catgatacgg taattagatg ggttatttga
      361 tttttgctga cgtttgaagc cagagatcgc attaaacttt ctttagcaag tccgatagca
      421 gatttattat aataatccat cctatcctga acctccggcc atggttgact tttggcatct
      481 tttggcataa atttcctttc atctttatca caggaaaaat ccggcaacac agaatagcgg
      541 gtttttatac cgctccgttc atataataat tttatttttt ccaataaata tttcaactat
      601 gaaaactttt ttcaattcaa ttccattcat tatttacatt caggttgaac tcctatttgt
      661 actatcactt ttaagttcga gaatattagt tgcaagcttt tttagaaaca tcctttgtgc
      721 taagtttttt tccgataatt tatatttcac ttttacaaaa acaaccaaac tattttctac
      781 actacgcgta aagcaagaga tatcgacaat gctgagccat tgtgcattac ctattttata
      841 cacaacaatt tagtttcgag ttgacagctt caaacttcaa acttcaaact tttaacttca
      901 aactaaaacc atctcagcca aattttattt atctttatac caatgtagca aattgctata
      961 agtatttata taaaaatata ttctcttaat aaattctttc atcatgggta agtatttgtt
     1021 tgcagtattg ttattggctt gcagctttgt tttgcatgcg caaattaatt tagtgcctaa
     1081 tccgagtttt gaacaatatg tgagttgccc aaataataca ggacaagttg atagctgtgt
     1141 tggttggtat agtgttttag aaacaccaga ttattataat acatgtgccc cttatcctgt
     1201 taacatacca aataatgtat gtggatacca aaatagtttt gatggatttg ggtatatggg
     1261 cttacatact tttacatgga tacctgtaaa ttatagggag ataatcggtg cagctttaat
     1321 tgatacaata gttgtcggta atacctacaa tatctcaatg cgtgtgagta gaggaaattg
     1381 gactattcaa gcttacaatt gttctgcaag taataaattg gggatacttt ttacaaccaa
     1441 tccaataacc aaattaacaa ttagcgatat aaataatttt gcaagtgttt atactgatag
     1501 cattattact gacacattaa attgggttat gttgtcttgg agttttgttg ccgattctgc
     1561 atacacaggc ttgtacatcg gtaatttttt tgacgacaac aatacagata cccttgttat
     1621 tgctgcacca ttaggccagt ttggttatgc ttattactat atagatagca taaatgttta
     1681 ttgtacttct ggtaattgca atgttggagt gaaagatatt actacaaatc atataagcgt
     1741 tttttacaat aatacaagca atgaattgaa cgtaattttt gaaaataata gcgaattcaa
     1801 agtttcgatt ataaatacat tagggcaaat ttgtaaaact gttagtattt cgataaccca
     1861 aaaatttact ctgccaaaac tttctcaagg aatttattat gctgttttgc agtcagcaaa
     1921 aaatactgta gttaaaaaat ttactattca ttaaaaaata aataatatga aaaaaataat
     1981 tggatttata atattagctg cacttggggc aattacctta aatgcgcaaa caccttggta
     2041 tttgattgga aatgcgcctg ttattggcaa cttccttggc actaccaatg cccaaagcct
     2101 gcaattgaaa acggaggcaa gccaacccat caatctctac acctcggcag gggtgggcac
     2161 attcaacact ggccgtttga ccataaaagg tagctatgta ggcacgagtg gtaatggttt
     2221 aataggcata ggtgatatgg gtactaattg tgctttttta cctcaaaatt tattgcatca
     2281 acatactaca tcaatcacac gaaccaactt tcatcagttt acaaatcctg caacgggttc
     2341 aggtgctaat aatggtttct tatttggtca acctgctaat agcttaactg gagaaatacg
     2401 gcaacaagaa gatgcaccca ttattattta tacaggtaat ttaaatgcag cacaaatgct
     2461 ggagcgtatg aggtttacta ctggcttggg tggcgatggt gtaggcaatt ggaatacgat
     2521 ttcaaatgca acaaaaataa atattagtag tggcggtatc gcaactgtgc cgttcaactt
     2581 tccagttgcg tgtttaaata ttggttatga tttaccaggc aatgcaagtg gtggccgcag
     2641 gccttggatg gatattggta cttactattc ttcaaactcc gatcacatgt acacaggctt
     2701 attggatatg ggcagcaatc gcaaagatgc cattgtaaat tttggtgacg acccacaata
     2761 caatgcattt gacaatagtg tacaaaaccc cgaaaacatt cggggcttaa tttatttcaa
     2821 tacaatttca atacacaaaa aactacatac cactatcatc agccgaaggg ccataactac
     2881 tcggcaccgg tatattattc aaacgtaaat acacacccaa ttgtgcgcga tgatgtacat
     2941 gatgattcat tacccatgtg cgcaccactt gcaattttgg cagggtaaaa aatatttgat
     3001 cgccactgcg catggtccag ttttcggcaa acatatcatc ggtagctttg ctcaaaattt
     3061 cgatggcttc ggcactgcct ttatcaaatt tttctaacaa ctcctctgtg gttgtgagta
     3121 taacaggttt aaaatcgccc gaggcaaaat ccaattcatg tttcaccaaa cagatattca
     3181 tccatccggg aatttcggca acgtgtgtgg caaggcggcc cattgtcatc gatttttcgt
     3241 ggggtttaaa ttcggcaagt ttcatatcta cacttgcaag tattttgcgc gtagttgcca
     3301 attccaattt gtaatcgggc aaaaatattt ctgctattgt catctttttt ttgtttttaa
     3361 aaatttatgc gaagatgaaa aaaatcagcc atccacatgt taacaatttt gatttatgtt
     3421 ttgtaaaaaa atacatccat aaaaaaaggc tgccaaaatt tgacagcctc tttttaattt
     3481 tattttctcc caaaattact ccactgtaag tggacgggtt actggttttt cgttaggtgt
     3541 ttctattttc aaaatgtatt tgcctttact caaattcgcg tcattgatac ttacaatatt
     3601 tattcctgca ttgatgt
//
LOCUS       DAORQA010000186         3615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6223_length_3615_cov_24.013202, whole genome shotgun sequence.
ACCESSION   DAORQA010000186 DAORQA010000000
VERSION     DAORQA010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6223_length_3615_cov_24.013202"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PK736_07865"
     CDS             <1..468
                     /locus_tag="PK736_07865"
                     /inference="COORDINATES: protein motif:HMM:NF025720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HRC33344.1"
                     /translation="LLFDKKRTVDSLKASVKRGSYDGFDKRLAALGNVSAKNSTISIA
                     SIFVMLLFLAIEIAPVLVKLLSSRGPYDDLLEAHENTFHSFKQEQVSKRNQKMYEKLL
                     EMSETGNHAVNERIETNIELRKRITEAEKEIAQAMIKQWKYEQMNKTRTTADY"
     gene            complement(549..1160)
                     /locus_tag="PK736_07870"
     CDS             complement(549..1160)
                     /locus_tag="PK736_07870"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRC33345.1"
                     /translation="MSTTFNKIDSANYYNNLSMLFFTRKNSNYIYQNYDFKYRILKGQ
                     KNYAEAIKFLELSIANKDSLENRENLQKSYDIIMFASGIPVLEKSTAIDAIKGCNDII
                     QYVKLVKQNANGNDNITSFDIRIGVNSGPLIAGIIGKKKFAYDIWGDTVNVASRIEGI
                     CEPGQINISESTYNLVKTHFKCKFLNKMTLKNRSDVNVYCIGN"
     gene            1331..2164
                     /locus_tag="PK736_07875"
     CDS             1331..2164
                     /locus_tag="PK736_07875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC33346.1"
                     /translation="MNVESISTAKFISKNGILYNSSDTNLSYPSEGNEMCFQLEDNSY
                     WFKHRNRIISLMIAKYKAHRNVFADVGGGNGFVSKLLQTQLADVILIEPGPQGVINAS
                     KRGVKNVICSVLDQCKFINKSIEMIGMFDVLEHIEHPNNILQESKRVLTDDGLLFITV
                     PAFNFLWSNEDKSAGHFTRYTKSTMKNLLHTNGFEPVFMSYFFQFLTMPIFALRSIPS
                     RLGMYARQQNQQHYNKQHSSGSGISNSIVNYFCNKEEATLSAGNQLNFGSSLIVVAKK
                     K"
     gene            complement(2177..>3615)
                     /locus_tag="PK736_07880"
     CDS             complement(2177..>3615)
                     /locus_tag="PK736_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRC33347.1"
                     /translation="TNHKIDFIFPATTYDLLLLSQHKLKFETEINCTIAICDEQYVKI
                     FLDKISTNQFLIDNGLNTPPLADYNEASHYPLIAKPKMGWGGKGLHVFKSLDAFVAAK
                     INDAADYLWQKLLSDFDEYSIDFSVNRQGIISDPVVRTRDATSGGFAVVSSTQSAVPE
                     NILPQLERVQKTFSQDCFCGLYNVQIIFASDHAYVTDVNPRIGTSAVMGNVIGKNLLH
                     NFFTAVQKQTNTPITTNFKVVRFLDEIYIPKIDTSKIKGIVFDFDDTLISNKDFIIAR
                     CALLYEKEKNIFNTNVHTFLQMAIAFLNEGKAHKLIDCLCEHFNVDAHQYQILESYRD
                     CFPESLSIYNDVGHVLQQLHHKGLQLFVLTDNPVRTQEIKLNLFPHRHLFTKVYFTGA
                     MENQKPHPGCFEIICKEYKLLPEELAMVGDNQYRDIIGSLDAGYKNAFYIKRSDSIVS
                     HSNNIFTNYKNPERIITIQNLKQLLYHF"
BASE COUNT         1156 a          622 c          670 g         1167 t
ORIGIN      
        1 ttgcttttcg ataaaaaacg aacagtagat agtttgaagg caagtgtgaa gcgcggttca
       61 tacgatggct ttgataaacg gctggccgct ttgggcaatg tatcggcaaa aaacagcacc
      121 atttctattg caagtatttt tgtgatgcta ttgtttttgg caatcgagat tgcaccggta
      181 cttgtaaaat tgctttcatc gcgagggcca tacgatgatt tgctcgaagc acatgaaaat
      241 acttttcata gttttaaaca agaacaggtt tcgaagcgca atcaaaaaat gtatgaaaaa
      301 cttttggaaa tgagtgaaac cggaaatcat gctgtgaatg aaagaataga aaccaacatt
      361 gaattgcgaa agcgcataac cgaagccgaa aaagaaattg ctcaagcaat gattaaacaa
      421 tggaagtacg agcaaatgaa caaaacacga acaacggctg attactaaat ggtttgttta
      481 ttatgattgt aattttggga ttgagatttt ttctgcgttt tatttaaaat atttccactt
      541 caaaaaattc aattcccgat acaatataca tttacatcgc tgcggttttt caatgtcatc
      601 ttgtttaaaa atttacattt gaaatgtgtt tttacaagat tgtatgtaga ctccgaaata
      661 tttatttggc ccggttcgca aattccttca atacgtgatg ccacatttac cgtatcgccc
      721 catatatcat aagcaaattt tttcttaccg ataattcctg caatcaatgg gccgctgttt
      781 acaccaatgc gaatatcgaa gctggtaatg ttgtcgttgc catttgcatt ttgttttacc
      841 aattttacgt attgaataat atcgttgcaa cccttgatgg catctattgc cgttgacttt
      901 tcaagcactg ggatgcccga tgcaaacata attatgtcgt aactcttctg aagattttcg
      961 cggttttcta atgaatcttt attggcaatt gaaagctcaa gaaatttaat tgcttcggca
     1021 taattttttt gccctttcaa tattctatat ttaaaatcgt aattctgata aatgtaattg
     1081 ctgttttttc ttgtaaaaaa aagcatcgaa agattgttgt aataatttgc cgaatctatt
     1141 ttattaaaag ttgtgctcaa ttgagtatca ctgttttttc gaatcaaact tctgcatgaa
     1201 aatgttatgt ttgagcccac tataaaaaca aagaaatttt aagttaactt ttcgaatgct
     1261 gtctaaattt aaaattgaca gtatttcgat tcgaaaaaat ttaacaagaa tataattgtc
     1321 aaaccaaagc atgaatgtag aatcaatttc aactgcgaaa tttatcagca aaaatggcat
     1381 tctatacaac agtagcgata ccaatctttc gtacccgagt gaaggcaatg aaatgtgttt
     1441 tcaactcgaa gacaacagct attggttcaa acaccgcaat cgtattatca gtttaatgat
     1501 tgctaaatac aaagcgcatc gaaacgtttt tgctgatgtt ggtggtggaa atggatttgt
     1561 gagtaagctt ttgcaaacgc aacttgccga tgttatactt attgagcccg gcccgcaggg
     1621 agtaattaat gcctcaaaac gcggagtaaa aaatgtaatc tgttcggtgc tcgatcaatg
     1681 taagttcata aacaaatcca tagaaatgat tggaatgttt gatgtactag agcacattga
     1741 acatccaaat aatattttgc aggaatcgaa acgggtattg accgatgatg gtttgttatt
     1801 tattaccgta ccggctttta attttctgtg gtccaacgaa gataaatcgg cagggcattt
     1861 tacacgttac acaaaatcga caatgaaaaa tttgctccac accaatggct tcgaaccggt
     1921 ttttatgtcg tacttttttc agtttttgac catgcccatt tttgctttgc gttcaattcc
     1981 atcacgctta ggaatgtatg cccgtcagca aaatcagcag cattacaata agcaacatag
     2041 tagtggcagt ggtatatcga acagcattgt aaattatttt tgcaataagg aagaggcaac
     2101 actttcagcg ggcaatcaat tgaattttgg aagcagcttg atagtggtag caaagaagaa
     2161 ataatcttca ctcattttaa aaatgataca acagttgttt taaattttga atcgtaataa
     2221 tgcgttcagg atttttgtag tttgtaaaaa tgttattgga gtgcgataca atgctatcac
     2281 ttcgcttaat ataaaaagca tttttatatc cggcatccaa tgaaccaata atatcgcgat
     2341 attgattatc gcctaccatt gccaattctt cgggcaacaa tttgtactcc ttgcaaataa
     2401 tttcgaaaca acccggatgt ggcttttgat tttccatagc gcctgtgaaa taaactttag
     2461 taaaaagatg acggtgtgga aatagattca gttttatttc ttgtgtgcgc acaggattat
     2521 cggtaagcac aaatagctgc aaacctttgt ggtgaagttg ctgaagcaca tgacccacat
     2581 cgttataaat tgaaagcgat tcgggaaaac aatcgcgata cgattcaagt atttggtatt
     2641 gatgtgcatc aacattaaaa tgttcgcaca ggcaatctat cagtttatgt gctttgcctt
     2701 cattaagaaa tgcaatcgcc atctgcaaaa atgtatgaac gtttgtgttg aaaatgtttt
     2761 tctctttttc ataaagcaag gcacaacgag cgatgataaa atctttgttt gaaataagcg
     2821 tatcgtcaaa atcaaaaact attcctttta ttttcgatgt gtctatttta ggaatatata
     2881 tttcgtcaag aaaacgcacc accttgaagt tggtggtaat tggtgtatta gtttgttttt
     2941 gaacggcagt aaaaaagtta tgcaaaagat ttttgccaat cacgtttccc atcacagccg
     3001 aagtacctat gcgcggattt acatcggtaa cataagcatg atcactggca aaaataattt
     3061 gtacattgta caaaccacaa aaacaatctt gtgaaaatgt tttttgtact ctttctaatt
     3121 gaggcaatat attttctgga actgcacttt gtgtgcttga tacaacagca aagccgcccg
     3181 aagtagcatc gcgagtgcga acaaccggat ccgaaattat tccttgccgg ttcacactga
     3241 aatcaataga gtactcatca aaatctgata gaagtttttg ccataaataa tcagcggcat
     3301 cgttaatctt tgcggcaaca aacgcatcaa gagatttaaa aacatgaagg ccctttccgc
     3361 cccatcccat ttttggtttt gcaataagtg gataatgcga tgcttcattg taatcggcca
     3421 aagggggtgt gttcagccca ttgtcaatta gaaactgatt ggtgcttatt ttgtctaaaa
     3481 atatttttac atattgctca tcacatatgg ctatggtgca atttatttct gtttcgaatt
     3541 ttaatttgtg ctgcgacaat aaaagcaaat cataagttgt ggcaggaaaa ataaaatcaa
     3601 ttttgtgatt ggtgc
//
LOCUS       DAORQA010000187         3605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6241_length_3605_cov_9.661972, whole genome shotgun sequence.
ACCESSION   DAORQA010000187 DAORQA010000000
VERSION     DAORQA010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6241_length_3605_cov_9.661972"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1419
                     /gene="serS"
                     /locus_tag="PK736_07885"
     CDS             142..1419
                     /gene="serS"
                     /locus_tag="PK736_07885"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRC33348.1"
                     /translation="MLSLLVIREQTEEIISRLERKNFKDARAIILQVLELDNSRKAFQ
                     KQNDDNLSQQNVLANKIGDLFKAGNKDEAAGAKAEVATLKAAAEEIKAKLTQTETDIY
                     ALMVTIPNAPSMAVPIGKTPEENEVISTNVKELPVMAEGSVPHWELTTKYDIIDFELG
                     NKIAGAGFPVYKGKGARLQRALINFFLDKATDAGYTEIQPPILINSDSGFGTGNLPDK
                     EGQMYHCQIDDLFLIPTAEVPVTNIYRDVILKESDLPQKLTAYTPCFRREAGSYGKDV
                     RGLNRLHQFDKVEIVQIQHPNKSYETLETMRDYVIGLLTELELPYRVLKLCGGDMSFS
                     SALTYDLEVYSAAQQRWLEVSSVSNFESFQANRMKCRFKDADGKTKLVHTLNGSALAL
                     PRIVAALLENNQTPTGINLPKVLHKYTGFETIE"
     gene            1887..2627
                     /locus_tag="PK736_07890"
     CDS             1887..2627
                     /locus_tag="PK736_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC33349.1"
                     /translation="MNHIFEIGRYWIFMKNLMHTPEKWRMYWTQILREMESMGFGSLP
                     IVAINSVFMGAVITIQSAYNIVNPMVPLSAIGIVTRDSIIIEFAPTVMMMVLAGKIGS
                     AIASEIGTMRVTEQIDALEIMGINSSTYLVLPKLIASLFILPFVVIISMFLGIFGGWI
                     AGDATGACPSADYLKGLQDQFLAFNVYFAMIKTLVFSYIIITVSSYHGYFTEGGSLEV
                     GKSSTKAVVYSNILILLFDYILTQLFLA"
     gene            2624..3391
                     /locus_tag="PK736_07895"
     CDS             2624..3391
                     /locus_tag="PK736_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC33350.1"
                     /translation="MIEVKNISKTFSTKQVLFDISCKFEPGKINLIIGTSGSGKTVLM
                     KTLIGLFTPDSGTIIYDGRDFIQLDRDGRKDLRQDIGMVFQGGALFDSLTVEENVMFP
                     LSMLTDMSLAEKRDRVNFCLKRVNLENAHKLFPAEISGGMKKRVSIARAIATNPKYLF
                     CDEPNSGLDPYTSLVIDELIHEITLEFNITTVINTHDMNSVAESGEHIVFLHKGAKWW
                     EGTFTEIMNSDNKELHGFIFASKVMQSFKKLYLKENA"
BASE COUNT         1158 a          675 c          745 g         1027 t
ORIGIN      
        1 tttcaattgg cggggtataa gccactcctt tgaagggtat ggggaggctt cctcaacttt
       61 catttcctcc caaaaactat cttccaatta taaatttcaa aaaactacct ttggcgttcg
      121 atttttttta aaactgagta tatgttaagt cttttggtaa tccgcgaaca aacggaagaa
      181 attataagtc gcctcgagcg caaaaatttt aaagatgcgc gggcaattat tttgcaagtg
      241 ttggaattag acaattcgcg caaggcattt caaaaacaaa atgacgataa cctgtcgcag
      301 caaaatgtat tggcaaataa aattggcgat ttgtttaaag ccggcaacaa agacgaagcc
      361 gcaggtgcaa aggccgaagt ggccacgcta aaagcagctg ccgaagaaat aaaagccaaa
      421 cttacacaaa ccgagactga tatttatgca ttgatggtaa ccataccaaa tgcaccaagt
      481 atggcagtgc ccattggcaa aacacccgaa gaaaacgaag taatatccac caatgtaaag
      541 gagttgcccg ttatggcaga aggcagcgtt ccgcattggg aacttaccac caagtatgat
      601 attatagatt ttgaattggg aaataaaatt gccggtgcag ggtttccggt ttacaaaggt
      661 aaaggtgcaa gactgcaacg tgcacttatc aatttttttc tcgataaagc aactgacgca
      721 ggctataccg aaattcaacc tcccatttta attaacagcg attcgggatt tggtaccggc
      781 aacttgcccg ataaagaagg gcaaatgtac cattgccaaa tagacgattt gtttttgata
      841 cctactgccg aagttcctgt tacaaatatt tatcgcgatg tgattttaaa agagagcgat
      901 ttgccacaaa aactaactgc gtacacacca tgctttaggc gagaggcagg ttcgtatggt
      961 aaagatgtac gaggattaaa tcgtttgcat caatttgata aagtagaaat tgtacaaatt
     1021 cagcatccca ataaatcata cgaaacgctt gaaaccatgc gcgattatgt tattggttta
     1081 ttgacagagt tagaattgcc ttatagggtt ttaaaacttt gtggtggtga tatgagtttc
     1141 tcttcggcac tcacttacga tttagaagtt tacagtgccg cgcaacaacg ttggttagaa
     1201 gttagctcgg tttcaaattt cgaaagcttt caggccaacc gtatgaagtg ccgttttaaa
     1261 gatgccgatg gcaaaacaaa actggtgcac accttaaacg gaagtgcatt ggcattgccc
     1321 cgcatagttg ccgcattatt agaaaacaat caaactccaa caggaattaa tctgccaaaa
     1381 gtgttgcata agtatacagg cttcgaaacg atagagtagt tcaagagttt aagggtttaa
     1441 tagtttaatg gtttaagagt tcaagagtta tgaaagttta gaaagttgag aaggtttata
     1501 aagttcaagg gctactgaaa tttgaaattt tctttggttt atattaaagc cccttgaagg
     1561 gcctgcaccg accgaagtgt cagtgtttgg ggtgtatttc aaaaaataaa tcaaaaggat
     1621 tgagtcaaaa ttcaaaatct aaaatggcac caaaacaaaa ctaaaaaatg agaactacgc
     1681 agagattgct tctcagttcg agaatccctt cgactgcgct cagggtaaca acactcactg
     1741 tgacaattcg caatgacgga acaatgcaat gcttaccaaa ttcgaatcta aaaactaaac
     1801 gaaattttaa actaaaaact gaattccgaa actaagaact aagaacaagg ctcacagcaa
     1861 acgtaacaaa gaacaaaaaa ctaaaagtga atcacatatt tgaaatagga cgctactgga
     1921 tattcatgaa aaacctgatg cacacgcccg aaaagtggcg aatgtattgg acacaaattt
     1981 tgcgtgaaat ggaaagcatg ggctttggct cgttgcctat tgttgctatc aattctgtgt
     2041 ttatgggtgc agtaattacc atacagagtg catacaacat tgtaaaccca atggtgccat
     2101 taagtgcaat aggcatagta acccgcgaca gtatcattat agaatttgcg ccaacagtaa
     2161 tgatgatggt gcttgccggc aaaattggaa gtgccatagc atcggaaatt ggaaccatgc
     2221 gtgtaacaga acaaatagat gcgcttgaga taatgggcat caacagcagc acttatttgg
     2281 ttttgccaaa actcattgca tcacttttca tacttccatt tgtagttatc ataagtatgt
     2341 ttcttggtat ttttggtggg tggatagctg gcgatgccac gggtgcatgc ccttcggccg
     2401 attatcttaa aggattacaa gaccagtttt tggcgttcaa cgtttatttt gccatgataa
     2461 aaactttggt gttctcctat atcatcatca cggtttcgtc ttaccatggt tactttaccg
     2521 aaggcggttc gctcgaagtg ggcaagtcgt caaccaaagc agtggtgtat agcaacatcc
     2581 ttattttatt gttcgattac atacttacac aattattcct ggcatgatcg aggttaaaaa
     2641 tatttctaaa acattctcca ccaagcaagt tttgtttgac ataagctgca agtttgagcc
     2701 gggcaaaata aacctcatta ttggcaccag tggttcgggc aaaacggtat tgatgaaaac
     2761 cctgatagga ttgtttaccc ccgatagcgg cacgattatt tatgatggcc gcgattttat
     2821 acagctcgat cgtgatggcc gcaaagattt acggcaagat ataggtatgg tgtttcaggg
     2881 tggtgcatta ttcgactcac ttacggttga agagaatgta atgtttcccc tttctatgct
     2941 aaccgatatg tcgctggccg aaaaacgcga ccgggttaat ttttgtttga agcgggtgaa
     3001 cttagaaaac gcccacaaac tttttcctgc cgaaattagt ggaggaatga aaaaacgtgt
     3061 gtccattgca cgtgccattg ctaccaatcc aaagtatctt ttttgcgatg aacccaactc
     3121 cggcctcgac ccatatacat cgttggtaat agacgaactc attcacgaaa ttacactcga
     3181 gtttaatatc accacggtaa taaatactca cgatatgaat agcgttgccg agtcgggcga
     3241 gcacatcgta tttttgcaca aaggtgccaa atggtgggag ggtactttta ccgaaattat
     3301 gaatagcgat aacaaggaac ttcacggttt tattttcgca agcaaggtaa tgcagtcgtt
     3361 caaaaaattg tatttgaagg agaatgcata gtatcttttt tgatttttaa gacggcttca
     3421 ggtgtaaata ataatttcaa ttttgtgttt taatattttt tataaataat tataaatcaa
     3481 taccataatg atattgttgt aatcgatact tttatataca tttttttgac aaattaatgt
     3541 tggctcccca actatttcat tttcgctgtg aataaaacta aggcaatgaa tctaaatcca
     3601 agccc
//
LOCUS       DAORQA010000188         3551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6362_length_3551_cov_7.393593, whole genome shotgun sequence.
ACCESSION   DAORQA010000188 DAORQA010000000
VERSION     DAORQA010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6362_length_3551_cov_7.393593"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>217
                     /locus_tag="PK736_07900"
     CDS             <1..>217
                     /locus_tag="PK736_07900"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 family protein"
                     /protein_id="HRC33351.1"
                     /translation="MIMLLIAFSTSSCDTLMKTANTVLAVPTDAEVVGGLKDALSQGF
                     TNGVSVLSAAGGFNKNAAIRILLPPEAQ"
     assembly_gap    218..227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <228..696
                     /locus_tag="PK736_07905"
     CDS             <228..696
                     /locus_tag="PK736_07905"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HRC33352.1"
                     /translation="LRGLGMGAQVDRVIDKLNEGAENAVKTAKPIFVDAVKKMSFKDA
                     MGILTGGKSSATNYLKSTTTTALTGLFKPEIQKALDQVGVTKYWKDLVTTYNALPTVQ
                     NKLNPDLNGFVTEKALSALFKKVEDEENLIRANPAKRLTGLMKRAFAYADTKK"
     gene            complement(707..1165)
                     /locus_tag="PK736_07910"
     CDS             complement(707..1165)
                     /locus_tag="PK736_07910"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HRC33353.1"
                     /translation="MIVYRIAALPFCMDLSGKGAELYGGRWNNIGNAAIYTSQNISLA
                     LLEILCHTSFQKLPSNMQIVHIEIPAKIKIKNFGVDELPTDWNKSPASIVVKNFGTQL
                     LQKKEFAAYSFPSAVLPNEINFMLNPQHKLYSNIKIIKTEPLPFDNRLVK"
     gene            complement(1172..1660)
                     /locus_tag="PK736_07915"
     CDS             complement(1172..1660)
                     /locus_tag="PK736_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HRC33354.1"
                     /translation="MKPKVNEMSEPIALYAYPNTFFDLLQNILGGKKAVPHNFENEMD
                     LVRQSEKGIKMISVKSVSDFLGISLEKMSQLLNVSLRTFQRKKEQDIMSASISQKTID
                     LAEVAAKGMEVFGDKAIFNSWLTEEVKSLGNKKPIELLNTSTGTRLVMQTLGRLQYGV
                     YA"
     gene            1906..2478
                     /locus_tag="PK736_07920"
     CDS             1906..2478
                     /locus_tag="PK736_07920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRC33355.1"
                     /translation="MSYSAHVSAIIDDGCEIGEGTKIWHFTHIMPDCIIGKNCNLGQN
                     VVVSPQVTLGNNVKVQNNVSIYTGVICEDDVFLGPSMVFTNVINPRSAIARKNEYKQT
                     LVKQGASIGANATIVCGIKIGRYAFIGAGAVVTKNVPDYALLIGNPARQAGWMSEYGH
                     KLIFDKNGNALCEESGEKYKLLNNIVTKIS"
     gene            2475..3512
                     /locus_tag="PK736_07925"
     CDS             2475..3512
                     /locus_tag="PK736_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRC33356.1"
                     /translation="MNTNSNINFAIIGCGHIGKRHIEMLMRNTECKVIAACDILPEKT
                     INTQYEGVKYYADAKQMLLDNPLIDVVCICTPNGLHASQALMCLDAGKHVVIEKPMAL
                     SKADCEKIIFKSLQVSRHVFCVMQNRYSPPSVWLKEIIETKTLGDVFIVQLVCYWNRD
                     SRYYKGDTWHGTADLDGGTLFTQFSHWIDLLYWLFGDIKDILTTFADYNHATTTAFED
                     SGIVQFKFENGAMGSINYSTSVWDKNLESSLTVIGSKGSIKVGGQYMNEVEYCHIENY
                     TMPSLEPTNPANDYGAYKGSANNHHLVFSNVVNTLKGKTSATTNALEGLKVVEIIERI
                     YKHNTLNKKGK"
BASE COUNT         1180 a          640 c          693 g         1028 t
ORIGIN      
        1 atgataatgt tgctgattgc ttttagcaca agcagttgtg atactcttat gaaaacagca
       61 aatactgtat tggctgtgcc cactgatgcg gaggtagttg gagggttgaa agatgcttta
      121 tcgcagggtt tcacaaatgg tgtgagtgta ttatcagctg caggtggatt caataaaaat
      181 gccgccatac gaatattgct gccacccgaa gcacaaannn nnnnnnnttt acgcggatta
      241 ggcatgggtg cccaggtgga cagggtaata gacaaattaa atgaaggagc tgaaaacgca
      301 gtaaaaacag ccaagccaat atttgtagat gcggtaaaaa aaatgagctt taaagatgca
      361 atgggcatac ttaccggagg caaaagctca gcaactaatt atttaaaatc gacaacaaca
      421 acagcactta caggtttgtt caaacccgaa attcagaaag cactcgacca agtaggtgtt
      481 acaaaatatt ggaaagattt agttacaaca tacaatgcct tgccaaccgt acaaaataaa
      541 ttgaaccccg acctaaatgg ttttgtaacc gaaaaagcat taagcgcatt gtttaaaaaa
      601 gtggaagacg aagaaaacct cattcgtgcc aacccggcaa aacgattaac ggggttgatg
      661 aaaagggcat tcgcttatgc tgatacaaag aagtgataaa agcaaattac ttcaccaatc
      721 gattatcaaa aggcagcggt tcggttttta taatcttaat attgctatat aatttgtgtt
      781 gtggattcag cataaaattt atttcatttg gcaacactgc cgaaggaaaa ctataagcag
      841 caaattcttt tttttgtagc agctgagtgc caaagttttt tacaacaata ctggctggtg
      901 atttattcca atcagtagga agctcatcaa caccaaaatt ttttatttta atttttgcag
      961 gaatttcgat atgaactatt tgcatgttag atggcaactt ttgaaacgaa gtgtgacata
     1021 aaatctctaa caatgccaac gaaatatttt gcgaagtata aattgcagcg ttgccgatat
     1081 tgttccatcg ccctccgtat agctcagcgc ctttgcccga taaatccata caaaaaggta
     1141 acgcagcaat tcggtataca atcataaaac attaagcgta cacgccatat tgtaagcgcc
     1201 caagggtttg cattacaagc cttgtaccgg ttgatgtgtt gagtaattca atgggtttct
     1261 tattgccaag agattttact tcttctgtta accatgaatt aaatatggcc ttatcaccaa
     1321 atacttccat gccctttgcg gcaacttcgg caaggtcaat tgttttttgt gaaatacttg
     1381 cactcattat atcttgctct tttttacgtt gaaaggtgcg caacgataca ttcaaaagct
     1441 gactcatttt ttctaatgaa attcccaaaa aatcggaaac ggattttacc gagatcattt
     1501 ttatcccttt ttcggattgg cgaactaaat ccatttcatt ttcaaaattg tgaggcaccg
     1561 cttttttgcc gccaagtatg ttttgcagca aatcaaagaa tgtatttggg tatgcgtata
     1621 acgcaatagg ttcagacatt tcgttcactt ttggtttcaa agcgctgtat tttttcgttt
     1681 tttccacgca caaatataca ttcatttcga caattgtcaa ttattttacg acatttttca
     1741 cacttttttt ccattcaata tattctggtt tcttgtaaga tattttctca aatgccaaat
     1801 gctaaaacca gggagtaatt aatggctgag atttttttac ttttgcccca atgttgatag
     1861 cataaagcat gataaactat ttacagcatc aaatttgaaa tcacaatgag ttattctgca
     1921 catgtatctg ccataataga tgatggttgc gaaattggcg aaggcaccaa aatatggcac
     1981 tttactcaca ttatgcccga ttgtattata ggcaaaaatt gcaacctggg ccaaaacgtt
     2041 gttgtatcac ctcaagtaac attgggcaac aatgtaaagg tgcaaaacaa tgtaagcatt
     2101 tataccggtg taatttgcga agatgatgtc tttctgggcc caagcatggt gtttaccaat
     2161 gtgataaacc cacgcagtgc cattgcccgg aaaaacgaat acaaacaaac attggtgaag
     2221 caaggtgcaa gcataggtgc aaatgcaact atagtatgcg gtatcaagat agggcgatat
     2281 gcatttatag gtgcaggggc tgttgtaacc aaaaatgttc ctgattatgc tttattaata
     2341 ggaaacccgg cacgtcaagc aggatggatg agcgagtacg gacacaaact tatttttgac
     2401 aaaaatggaa atgctttgtg tgaagagagt ggagaaaaat ataaactact aaataatatt
     2461 gttacaaaaa tttcgtgaac acaaactcca acataaactt tgccattata ggctgcggtc
     2521 atataggcaa acgccatatc gaaatgctca tgcgcaatac tgaatgtaag gtgatagctg
     2581 catgtgatat tttaccggag aaaaccatca acacacaata tgaaggagta aaatattatg
     2641 ccgatgcaaa acaaatgctg ctcgataatc ctttgataga tgtggtttgc atttgcaccc
     2701 caaatggttt gcacgcgtca caagcattaa tgtgccttga tgcaggtaaa catgtagtaa
     2761 tagaaaaacc catggcgcta tcaaaggccg attgcgaaaa aataattttt aaatcgctac
     2821 aggtttctcg ccatgttttt tgtgtgatgc aaaaccgtta ttcacctcct agtgtatggt
     2881 tgaaagaaat aatagaaacc aaaactctgg gcgatgtttt tattgttcag cttgtatgtt
     2941 actggaaccg tgatagtaga tattacaaag gcgatacatg gcatggaact gcagatttgg
     3001 atggaggaac tttgtttaca cagttttcgc attggataga tttattgtat tggctctttg
     3061 gagatattaa agatattctc acaacctttg ccgattataa ccacgcaaca acaactgctt
     3121 ttgaggattc gggaatagta caattcaaat ttgaaaacgg ggcaatgggc agtataaatt
     3181 actcaacatc ggtatgggac aaaaatctcg aaagcagcct cacggtaatt ggcagcaaag
     3241 gaagcattaa agttggcggt caatatatga acgaagttga atattgtcat atcgaaaact
     3301 atacaatgcc aagtcttgaa cccaccaacc ctgcaaatga ttacggggca tacaaaggtt
     3361 cggcaaacaa tcatcatctt gtttttagta atgttgtaaa tacacttaaa ggcaaaacca
     3421 gtgcaactac caatgccctc gaaggactaa aggtggttga aataattgaa cgcatttaca
     3481 aacacaacac tctcaataaa aaagggaaat aataattgta aggtatacaa tttaaaaata
     3541 aacattgttt c
//
LOCUS       DAORQA010000189         3543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6383_length_3543_cov_8.772362, whole genome shotgun sequence.
ACCESSION   DAORQA010000189 DAORQA010000000
VERSION     DAORQA010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6383_length_3543_cov_8.772362"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PK736_07930"
     CDS             <1..543
                     /locus_tag="PK736_07930"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC33357.1"
                     /translation="IYNKVISLMHTHIEGFAFKAKIKKYVQQTQEIENSFWNCISRLY
                     SATFLNKQIFTGEILHIKSAEKKTITANTSEKKEKGATYVDTLNLFNEGKSLQEIAEI
                     RSIVLSTVQSHIAKWILEGAIDIHKIFPQEKVERVMKFIQDNPNDTIGNLKSHFGDAY
                     DFPELRMLMNHHIWNKKNNN"
     gene            581..2011
                     /locus_tag="PK736_07935"
     CDS             581..2011
                     /locus_tag="PK736_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRC33358.1"
                     /translation="MNLSITDKERNAILGELKTANIAFQNIYSGDKPDRQPVHTVYGG
                     ANLFKSDSIIKLGLSALKSFTTNAENFVVLAKLLETDGYKLLPKKDADIEKLISKLDK
                     MKLDERKKHPAWLPYTVYNKIITKLRIEAVEDFRIDFEDGFGNRSDAEEDATALAAAT
                     ALAKGMQEKTISPFIGIRIKPFTEDLKHRGVRTLDIFLSTLLPLTKNKLPNNFVVMLP
                     KVTITAQMETLVKFFEIIEKKHKLAPGTLKMEMMVEATQAVMDAEGKNPLLRLIQASK
                     GRCIAAHFGTYDYTASCNITAKYQTMAHPVCDFAHHMTRVALGGTGIMLSDGATNVMP
                     VGPHRGDKLTKKQLHENREVVHRAWKMAYNHTMHSLINGFYQGWDLNPAQLPMRYAAT
                     YVFFLQSYEDAALRLKHFVEKSAQATLSGDVFDDAATGQGLLNYFLKALNCGAISETE
                     ALATGLTVAEIRSRSFYTILTNRRKK"
     gene            2264..3487
                     /locus_tag="PK736_07940"
     CDS             2264..3487
                     /locus_tag="PK736_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006070611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HRC33359.1"
                     /translation="MEPHVMEWLNLILRWTHVVFGIAWIGASFYFIFLENSLNRTENL
                     RDELAGNLWAIHGGGFYYVEKYKSAPPILPKHLHWFKYEAYFTWLSGFLLLTLVYFMN
                     AKAMMLDSSVSNIDETTAVFIGLGSLVVGWLIYDVMCHTALLQKKKLFGIIGFAIVVL
                     VSYILCQFLNGRAAFILVGALMGTIMAGNVFFCIIPSQKALVAAAKANQPVNPELGKI
                     AGLRSLHNNYITLPVIFIMISNHFPTTFGSSFNWIILAALTLASVAVRHYINLYEKGI
                     YAKWMIPFATVIILSLVIVTAPKPRKLSNNSSSTKGIISAKNLPPVKFTEIQTIFKKR
                     CIVCHSANPTDDVNVIAPNGIKFDNAEEIQKYAERIMIRVVQTETMPQGNKTNITEAE
                     RETIGVWIEQGALLK"
BASE COUNT         1165 a          658 c          701 g         1019 t
ORIGIN      
        1 atatacaaca aggtaataag ccttatgcat acacacattg agggctttgc atttaaagcc
       61 aaaataaaaa agtatgttca gcaaactcag gaaatagaaa attcgttttg gaattgcata
      121 tcgcgtttgt atagtgcaac atttttgaac aaacaaattt ttaccggaga gatattgcat
      181 ataaagtcgg ccgagaaaaa aacgataacg gcaaacacat cagaaaaaaa agaaaaaggt
      241 gcaacctatg tcgacacact taatttattt aacgaaggta aatcgttgca ggagatagct
      301 gaaatacgca gcatcgtttt atcaactgtt caatcgcata ttgcaaaatg gatactcgaa
      361 ggtgctatcg atattcataa aatatttcca caagaaaaag ttgagcgtgt aatgaaattc
      421 attcaggata atcctaatga tacaattgga aatttaaaaa gccactttgg tgatgcttat
      481 gattttcctg aattacgaat gttgatgaat caccacatct ggaataaaaa aaataataat
      541 taattggttt actttacttt tgaaacaaaa aaacatcgat atgaatttat caatcaccga
      601 caaagaacgc aatgccatat taggcgaatt gaaaacagca aacattgcat ttcaaaacat
      661 atatagtggc gataaacccg acaggcagcc ggttcataca gtatatggcg gggcaaattt
      721 atttaaaagc gacagcatta ttaagttggg attatctgca ttgaaaagtt ttacaaccaa
      781 tgcagaaaat tttgtggtgc ttgccaaact tttagaaaca gatggatata agttgctgcc
      841 aaaaaaagat gccgacatag aaaagctgat ttcgaagtta gataaaatga aattggacga
      901 gcgcaaaaag catcctgcat ggttgccgta tacggtgtac aataaaatta tcaccaaact
      961 tcgcatagag gcagttgaag atttccgcat cgattttgaa gatggatttg gcaacaggtc
     1021 cgatgccgaa gaagatgcaa ctgctttggc agcagcaacc gcactggcaa aaggaatgca
     1081 agaaaaaact atttcgccct tcatagggat tcgcataaaa ccttttaccg aagatttaaa
     1141 acatcgtggc gtacgcacac tcgatatttt cctttctaca ttattaccgc tgacaaaaaa
     1201 taaattgcca aataattttg tagtgatgtt gcctaaagtg actatcaccg cacagatgga
     1261 aacactggta aaatttttcg aaataataga gaagaaacac aaactcgcac ccggcacact
     1321 caaaatggaa atgatggttg aagccacaca agctgtgatg gatgccgaag ggaaaaatcc
     1381 attattgcga ttgatacagg catcgaaagg gcgttgcatt gcagcacatt ttggcaccta
     1441 tgattatacg gcatcgtgca acatcaccgc taagtatcaa acaatggcgc atccggtttg
     1501 cgattttgca catcacatga cacgggtagc acttggtggc acaggcatta tgctcagcga
     1561 tggcgctacc aatgttatgc ccgtaggtcc gcatcgtggc gataaactta ctaaaaagca
     1621 gctgcacgaa aatcgtgaag tagtacatcg tgcatggaaa atggcttaca accatactat
     1681 gcattcactt ataaatggtt tttatcaggg ttgggatttg aatcctgcac aattgcccat
     1741 gcgatatgca gctacttatg ttttcttttt acaaagctat gaggatgccg cattgcgttt
     1801 aaaacatttc gtagaaaaat cggcacaggc aacattgagt ggcgatgtat ttgacgatgc
     1861 tgccaccggc caagggttgc taaattattt tctcaaggcg cttaactgcg gtgccataag
     1921 cgaaaccgaa gcactggcaa ctgggttgac cgtggccgaa atacgcagcc gttcgtttta
     1981 tactatacta acaaaccgca gaaaaaaata gtccaccttt tttattttat acatgccaca
     2041 caatacagat attttctttt ttaccatttt cattatcatg gctgtggcat cgcttgttct
     2101 tcgaagagta tatgtatttg tcaacaggaa tagcactatt caaaatcacg aatcccattt
     2161 ggaaatgcat gcagaactga aaacttatgc caacgtgttt tatttttttg ctttgctaac
     2221 tgtacccata tacgcagcat atattgttac tgaaaaattt caaatggagc cacatgtaat
     2281 ggaatggctc aaccttatat tgcggtggac acacgtagta ttcggcattg catggattgg
     2341 tgcatcgttt tattttatat ttctcgaaaa cagtttaaac cgtaccgaaa acctgcgcga
     2401 tgaacttgcc ggcaatttgt gggctataca cggaggtgga ttctattatg tagaaaaata
     2461 taaatcggca ccgcccattt taccaaagca cttacactgg tttaagtacg aagcatactt
     2521 cacatggcta tcgggtttct tgttacttac acttgtttat tttatgaatg ccaaagccat
     2581 gatgctcgat tcatcggtaa gtaatattga tgaaactacg gccgtgttta ttggcttagg
     2641 aagtttggta gttggttggt tgatttatga tgtaatgtgc cacaccgctt tattacagaa
     2701 gaaaaaatta tttggcatta tcggttttgc aattgtagtt cttgtctcct acatactatg
     2761 ccaatttttg aatgggcgtg cagcatttat tctagttggt gctttgatgg gcaccattat
     2821 ggcgggcaat gttttctttt gcatcatacc ctcgcaaaag gctttggtgg cggcagcaaa
     2881 agccaatcaa ccggtaaacc ctgaacttgg aaaaattgcc gggcttcgtt cgctgcataa
     2941 taattacatc acactgccgg tgatttttat aatgataagc aatcattttc caacaacatt
     3001 tggcagcagt tttaactgga taatacttgc agcacttaca cttgccagtg tggccgtgcg
     3061 gcattacatt aatctttatg aaaaaggaat ttatgccaag tggatgatac catttgcaac
     3121 agtaattatt ttatcgcttg taattgttac tgcgcccaag ccacggaagt tatctaacaa
     3181 ttccagttca acaaaaggaa taatttctgc aaaaaatttg cctccagtaa aatttactga
     3241 gatacaaacc atttttaaaa agcgttgtat agtttgccac tcagccaatc caactgatga
     3301 tgtaaacgta attgcaccaa atggaattaa gtttgataat gccgaagaaa tacaaaagta
     3361 tgctgaacgc ataatgatac gtgtagtgca aaccgaaacc atgcctcagg gaaacaaaac
     3421 aaatattacc gaggcagaac gcgaaacaat tggcgtttgg attgagcagg gtgcattatt
     3481 aaaataatca tttatattat ttagatattg gaatttttgt ggtcaatatt ttgattatag
     3541 taa
//
LOCUS       DAORQA010000190         3539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6398_length_3539_cov_11.065729, whole genome shotgun sequence.
ACCESSION   DAORQA010000190 DAORQA010000000
VERSION     DAORQA010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6398_length_3539_cov_11.065729"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PK736_07945"
     CDS             complement(<1..815)
                     /locus_tag="PK736_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33360.1"
                     /translation="MMFGQTTYYWVGGLAAATGINTGTNWNTDINGAGTSRPSSTGAT
                     DILVFDGTNLGGATTATGPATILASSSITCAQVKFVNNVNITMLRPTSGTSTITIAGE
                     LGEDFVVNAGCTFNVASPVGSLRFTFQSAVDACRVSGTVSLITPWQMRFDNGTGGSPG
                     TFVFTNGSSFTTNITSTSSSYAFGSSSQSSEKWVVFQSGAHLYYLGGWSPMGNSSTFS
                     AINFEPGSFWHHRAAVAGGSYFNNKSFGNIIVENGSTLVADGPIHRINNLTVNT"
     gene            912..2891
                     /locus_tag="PK736_07950"
     CDS             912..2891
                     /locus_tag="PK736_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRC33361.1"
                     /translation="MKKLLSLILLIAVVCTGTVSMAQKKEKSVCAKADRMMDRGNYYE
                     AVNFYKKVYNKKKKASQKAQAANKAGECYMKMGDWKQAEEWYGKAAKANPKDANAVLM
                     HAKALQGVGRYDEATAEFNAYKAIAGTDATLADAGIQSAAQAQTWKDKPTRYRVENIG
                     ALNTKYYDYGTAISPGLPNTVFFTSSRIEAVGTENDNWYGEKFYDIFKSTKDNIGKWS
                     TPVPIEGEANSKHSDGSLCLDATGLIMYYTRAERIKGKETVGKIYKSTFSNGKWSEGE
                     SFAYNNDSITNGQPCLSPDGSVLYFVSTMPGGYGLHDIWMCKNEGGNWSTPVNLGSSI
                     NTAGDEMFPVAATNGKLYFSSNGLPGMGGLDIFSATQANGTWGDAKNLKANMNSPWDD
                     HSMVWTTDTSGYLTSGREGGQGQEDIYSFVYPPLILNVSGKVYDTDTKEAIEGATVEL
                     FGSDGTTLSLKTGPDGMYTYSLKENVRYKLSASYTGYLTKFHELSTEGLEENKEFNKD
                     FDFPLKSTSKAIVLNNIYYDLDKWFLRPESKDTLGAIVTTLEQNPTIVVEFSSHTDFR
                     ATDEYNQKLSERRAKSVIDYLISKGIPKDRLAYAGKGEKEPWVMTIADGGLKIGDVLS
                     ESFINGLANDADKEKAHQYNRRTQFKVLRTDYVPH"
     gene            complement(3290..3538)
                     /gene="purS"
                     /locus_tag="PK736_07955"
     CDS             complement(3290..3538)
                     /gene="purS"
                     /locus_tag="PK736_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRC33362.1"
                     /translation="MFCYIQDVFDYLKMQQGKAVSATMKNIGLPEIENVRIGKHITFE
                     ITANSASQAQSQAEEACRKLLANLIMEDYHVVVEEMGV"
BASE COUNT         1147 a          664 c          728 g         1000 t
ORIGIN      
        1 gtattaaccg taagattgtt gattctgtgt attggcccgt cagcaactaa agttgatccg
       61 ttctctacaa taatatttcc aaaacttttg ttgttgaaat aagagccacc ggcaacggct
      121 gcacgatgat gccagaaact tccgggctca aaattaatag ccgaaaaagt ggagctgtta
      181 cccattgggc tccatccgcc caggtaatat aaatgagcgc cactttgaaa taccacccat
      241 ttttcggatg attgtgaaga acttccaaat gcataagacg aagatgttga agtgatattg
      301 gtagtaaatg aagaaccgtt agtaaataca aaagttcccg gcgaacctcc ggtgccatta
      361 tcaaaacgca tttgccaggg tgttattagt gaaactgtac cgcttaccct acatgcatca
      421 acagccgatt gaaatgtaaa acgaagacta cccaccggcg atgccacatt aaatgtgcat
      481 cctgcattaa ctacaaaatc ttctcccaac tctcctgcaa tggttattgt acttgtaccc
      541 gatgtagggc gaagcattgt aatatttaca ttgttaacaa atttaacctg tgcacaggtt
      601 atgctggaac ttgccaaaat tgttgctggc ccggtagccg ttgttgcacc acccaggtta
      661 gtaccatcaa aaactaaaat atcggttgcg cctgttgatg aaggccttga tgttccggct
      721 ccattaatat ctgtgttcca gttagtcccc gtattgatgc cggtggcagc agcaaggccg
      781 cctacccaat aataggttgt ttggccaaac atcattccgg ttgtaaataa caggaaaatt
      841 gtaaaaaata tttttttcct ttgccactta tcctaaacat cctatttaaa ataatagaaa
      901 aacagtaata tatgaaaaaa ttactctcct tgattttgtt aatcgctgtg gtttgtaccg
      961 gaacagtgag catggcgcag aaaaaagaga aaagtgtatg tgctaaagcc gatcgaatga
     1021 tggaccgtgg caactactac gaagctgtaa acttttataa aaaagtttac aacaaaaaaa
     1081 agaaggccag ccaaaaggct caagctgcaa acaaagccgg agaatgctat atgaagatgg
     1141 gcgattggaa acaagctgaa gagtggtatg gcaaagctgc aaaagcaaat cctaaagatg
     1201 ccaatgctgt gttgatgcat gccaaggcac tgcaaggagt tggtcgctat gacgaagcca
     1261 ctgccgagtt taacgcatat aaagctattg caggaaccga tgccactctt gcagatgcag
     1321 gaatacaatc ggccgcacaa gctcaaacct ggaaagacaa gccaactcgt tatcgcgtag
     1381 aaaatattgg tgcgttaaat actaaatatt acgattatgg aactgccatt tctccaggat
     1441 tgccaaatac agttttcttt acatcttcac gtatcgaagc tgttggaact gaaaatgaca
     1501 attggtatgg agaaaagttt tacgatattt tcaaatcaac aaaagacaat attggcaaat
     1561 ggagtactcc tgttcctata gaaggagaag caaactcgaa acattccgat ggctctttgt
     1621 gcctagatgc taccggattg ataatgtatt acacccgtgc tgaaagaata aaaggaaaag
     1681 aaacagtagg taagatttac aaatctactt ttagcaatgg caaatggagt gaaggcgaaa
     1741 gttttgctta caataatgat agtattacaa acggccaacc gtgcttatct ccggatggaa
     1801 gtgtgttgta ctttgtttca actatgcccg gaggatatgg tttacatgac atatggatgt
     1861 gcaaaaacga aggtggaaat tggagtacgc ctgtcaacct tggttcaagt attaatactg
     1921 ctggtgatga aatgtttcca gtagcagcta ctaatggtaa gttatacttc tcttcaaatg
     1981 gattgccagg aatgggaggc ttagatattt tttcagccac acaagcgaat ggtacttggg
     2041 gagatgcaaa aaatttgaag gcaaatatga attctccctg ggacgatcac tcaatggttt
     2101 ggactactga cacaagtggt tacttgacat caggacgcga aggtggacaa ggccaagaag
     2161 atatttactc tttcgtatat ccaccattga ttttaaatgt gtcgggtaaa gtatatgaca
     2221 ccgatacaaa agaagccatt gaaggagcta ccgtagaatt atttggtagc gatggtacaa
     2281 ctttaagttt gaagactggg cccgatggaa tgtacactta ttcattaaaa gaaaatgtac
     2341 gctataaact ttcggcctct tacacaggtt atttaacaaa attccatgag ttaagtaccg
     2401 aaggtttgga agaaaacaaa gagtttaata aagacttcga cttcccttta aaatctacat
     2461 ctaaagcgat tgtgttaaac aatatctatt acgacttaga taagtggttc ctacgccctg
     2521 aatcgaaaga tacattgggc gctattgtaa ctacattgga gcaaaatcca accatagttg
     2581 ttgagttttc gtcacatacc gattttcgtg ctactgacga gtacaaccaa aagttatcag
     2641 aaagacgtgc taaatcagtt atcgattatt tgatttcgaa aggaattcca aaagacagat
     2701 tagcttatgc cggtaaaggc gaaaaagagc cttgggtaat gacaattgct gacggagggt
     2761 tgaaaatagg tgacgtcctt tccgagtcat ttataaatgg attggctaac gatgccgaca
     2821 aagagaaggc acatcaatac aatcgtagaa cacaatttaa agtgcttcgc accgattacg
     2881 ttcctcatta ataattgaac gtataattaa taaagcacgc ctccagtttt gggggcgttt
     2941 ttttttggca gttatattca gtgccaatga ttggatcaga ttgtactaat ttagatgatt
     3001 ataatcatga agcctatcag tatattcaat gagcaattat tagaattgct cttacaagtg
     3061 aatccaatta atcccaaatt atgcagtaga aggcgaggaa cgagccgaac tatctgcatt
     3121 agttgggttt atcccgattt agaaaatcac ccatttgtaa aatgcaatga aactaatagg
     3181 gttgatttta ttaatcgctt tgcaattgaa aatttcaatt gcataatctg ggttaattga
     3241 acttatcttc aattcagaca attgctaaaa gtaagctttg cagcaaaaat tatacaccca
     3301 tctcctcaac taccacatgg taatcttcca taatgaggtt tgctaataat ttacggcagg
     3361 cttcttctgc ttgcgactga gcctgggagg ctgagttggc tgttatttcg aaggtaatat
     3421 gtttacctat tcgtacattt tctatttcgg gcagtccaat atttttcata gtggcactaa
     3481 ctgctttgcc ttgttgcatt tttaaataat caaacacgtc ttgaatataa caaaacata
//
LOCUS       DAORQA010000191         3507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6473_length_3507_cov_12.017092, whole genome shotgun sequence.
ACCESSION   DAORQA010000191 DAORQA010000000
VERSION     DAORQA010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6473_length_3507_cov_12.017092"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1701
                     /locus_tag="PK736_07960"
     CDS             <1..1701
                     /locus_tag="PK736_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33363.1"
                     /translation="IVILAIYVAGIFLQKKFFTMSIPSRDIYKLCGILFLSIASIIIN
                     PNGIKLLASPFEIFSQVQQNKYTTELSSLSELSSWTKEIYIVGLAILLLLVFLIRRII
                     LKKKTSTVDLFAHFGVSYLLTIAAFCFLAYTAFRNLAFLSFILFPVWVYFFQLVIQQI
                     MLRKKALLLPAYYVAMFTGLAMYVSVVSNQYYKITNSRDSFGLGIPCNMHPQGVAQHI
                     EQKGLQNKKCFSDYLTSSYLMWRLQPNFKTYIDLRDLDVFTSDFFNDYLRTVNDYKYF
                     LHIDSIQHFDYVVLQRTQHDVLHAYLYNDSIYACTYVDQVAAVYEKTDAFSRADIFNY
                     CTDVKQNALSKFINLALNPLYSSNTFQNSNSELIAAQYYINVGRIDLADQRIQQALVK
                     YPDEKDKTQCALGNLFARRAIFIADPKQKSDFLMQAAGAYNTALQFNNKNFEAMVGLG
                     TSQFYLQNYNDALQTFGKAIELKSDDANAISNAIECSRAIADKFPDRKIEMQNLELKY
                     CKMAYNNESQNPVNIMNLGFALYKTGDTTAAKKYLSSVKNNEMLSEDNRSLVNKLLGY
                     "
     gene            complement(2340..3284)
                     /gene="paaA"
                     /locus_tag="PK736_07965"
     CDS             complement(2340..3284)
                     /gene="paaA"
                     /locus_tag="PK736_07965"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HRC33364.1"
                     /translation="MEATKIFTESDREEQFLARIDAGEIIEPKDWMPEKYRKQLIRMM
                     SQHAHSEIVGMLPEGNWITRAPSLARKASLIAKVQDEAGHGLYIYSATETLGIDRQEL
                     VDQFLHGKAKYSSIFNYPTLNWADIGAIGWLVDGAAIVNQTALAKCSYGPYARAMVRI
                     CKEENFHHKQGYQILAIMMQGSAEQRQMVQDAINRFWWPSLMMFGPSDTDSPNSAELM
                     RWKVKLYSNDELRQRFIDRTVPQAEAIGIKLPDDKLKFNETTRHYDWGEINWDEFKQV
                     VSGNGPCNKQRMKARKDAEVNGHWVREAASAYAAKHYN"
BASE COUNT         1038 a          681 c          661 g         1127 t
ORIGIN      
        1 attgttatcc ttgctattta cgttgccgga atatttttgc agaaaaaatt cttcaccatg
       61 tcaatcccat cacgggacat ttacaaattg tgcggcattt tatttttatc cattgcttcc
      121 attattatta atcccaatgg cataaaatta ttggcaagcc cattcgaaat attttcgcag
      181 gtgcagcaaa ataaatatac taccgaactt tcatcattaa gtgagttatc atcctggaca
      241 aaagaaattt atattgttgg ccttgcaatt ttattgttgt tggttttttt aatcaggaga
      301 ataattctaa aaaagaaaac atcaacggtt gatttgtttg cacattttgg tgtgagttat
      361 ttactcacca tagctgcgtt ttgttttttg gcctatacag cttttcgtaa tttggctttt
      421 ctgtccttta ttttatttcc tgtttgggtt tacttttttc aattggtgat acaacaaatt
      481 atgcttcgca aaaaagcttt actcttgcct gcttactatg ttgctatgtt cacgggcttg
      541 gctatgtatg tttcagtggt gagcaatcaa tattacaaaa taacaaatag ccgcgatagt
      601 tttggtttgg gaattccttg taacatgcat ccacagggcg ttgcacagca catcgaacaa
      661 aaagggttgc aaaataaaaa atgcttttcc gactatctca catcatcgta tttgatgtgg
      721 cggcttcaac caaatttcaa aacgtatatc gaccttcgcg atttggatgt gtttacctcc
      781 gattttttta atgactattt gcgtactgtg aatgattata aatacttttt gcatatagac
      841 agcattcagc atttcgacta tgtggttttg caacgaactc aacacgatgt gctgcacgct
      901 tatttataca acgatagtat ttatgcctgc acttatgttg atcaggttgc ggcagtgtac
      961 gaaaaaacag atgccttttc gcgtgccgat atttttaatt attgtaccga tgtaaaacaa
     1021 aatgctttat cgaagtttat aaatctggca ttaaacccac tgtatagcag caacacattt
     1081 caaaacagca attccgaatt aattgcagca caatattaca tcaatgtggg ccgtatagat
     1141 ttagccgacc aacgtataca gcaagcgtta gtaaaatatc ctgacgaaaa agataaaact
     1201 caatgtgccc tgggcaattt gtttgccagg cgtgccattt ttattgccga cccaaaacaa
     1261 aaaagcgact ttttgatgca ggctgcaggt gcatataata cagcattgca attcaacaac
     1321 aaaaacttcg aggctatggt gggtcttgga acatcgcaat tttatttgca gaattataac
     1381 gatgcgctac aaacctttgg caaagcaata gaacttaaaa gtgatgatgc caatgcgata
     1441 agcaatgcca tagaatgtag tcgtgctatt gccgataaat ttcccgaccg taaaatagaa
     1501 atgcaaaacc tcgaattgaa atattgcaaa atggcttaca acaacgaatc gcaaaaccct
     1561 gtgaacataa tgaatcttgg atttgcttta tacaaaactg gtgatacaac agccgcaaaa
     1621 aaatacttaa gttcggtaaa aaataatgaa atgctgagtg aggataatcg gagtttggtt
     1681 aataagttat taggttattg agttgttggg gattgtcatt tggcaataat tgtcattggt
     1741 cattgtgagt tttgttattg gttattgagg atagtcattt ggcaattctt gtcattggtc
     1801 attgtgaaat agttattggt tattttgttg ttgcctttgg catgctgagg tacgaagcat
     1861 ctctgcgtat tattagtcat tggtcattgt caccctgagc gaagtcgaag gggtcattgg
     1921 tcatgagtgc gcaagccttt gtttgtgtcc tcacaaacaa ttcccaagca aagagtaacg
     1981 gcatcgcgat aatggtttgt ggggacacaa accatggctg gcgcggattg gcttacaaag
     2041 gctcaattgg atgtattgaa atccatcctt acaaaattta gttctgctta cggaaatttc
     2101 agtgacacaa cattatttta agtatacgcg tattcctttc agggttagtt gaatgttgta
     2161 tagggctaaa aaaactgtgt gagaatttag aatattcaaa ataaaaatta ataatctatt
     2221 attctacaat caagacggag caaaaaaaca gtaaccatta actcaataac ataataacca
     2281 ataacacaac aacctacaac tcaataacca aataacacaa taacataaca accaaataac
     2341 taattataat gttttgccgc gtaagctgat gcagcttcgc gtacccaatg accattcact
     2401 tcggcatctt tgcgggcttt catacgttgc ttgttgcatg gtccattacc acttactact
     2461 tgcttaaatt catcccaatt tatttcgccc caatcatagt ggcgtgtagt ttcgttgaat
     2521 ttcaatttat catcgggtag ttttatacca atggcttcgg cttgtggtac cgtgcgatct
     2581 ataaagcgtt ggcgcaactc atcgttcgaa tataatttta ctttccatcg catcagctcg
     2641 gcactgtttg gcgaatcggt atcgctaggg ccaaacatca taagcgatgg ccaccaaaaa
     2701 cgattgatgg catcctgaac catttgccgt tgctcggccg acccctgcat cataattgcc
     2761 aatatctgat acccttgttt gtgatggaaa ttttcttctt tgcatatacg caccatagca
     2821 cgtgcatatg gcccatacga acatttggcg agagccgttt ggtttacaat tgctgcacca
     2881 tctaccaacc aacctatagc gccaatatcg gcccagttaa gtgtggggta attaaaaata
     2941 gaagagtatt tagctttacc atgcaaaaac tgatcgacca attcctgacg atcgataccc
     3001 aaggtttcgg ttgcactata aatgtataat ccatgtccgg cttcatcctg cacctttgca
     3061 atgagcgatg ctttgcgcgc caaacttggt gcacgtgtta tccagtttcc ttcgggcaac
     3121 atccccacta tttcgctgtg tgcatgctga ctcatcatgc gtatcaattg cttgcgatac
     3181 ttttcgggca tccaatcttt tggttctata atttcaccgg catcaattcg tgcaagaaat
     3241 tgctcttcgc gatctgattc tgtaaatatt tttgtggctt ccatattgtt aaatttcact
     3301 tgcaaagaaa agattatttt ttgcaattat taaccaacaa actatttggc aattagtttt
     3361 ttgattgctc ggggaaaaat ctgaatttaa aaaaaaggtt ggatccctat tccgcactta
     3421 agaatgtagc ttgatcaatt gccccggcaa atcattatca aaagcttgtg atttttttta
     3481 gggtcgcaac tatgagcagg aggcgaa
//
LOCUS       DAORQA010000192         3494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6496_length_3494_cov_6.617621, whole genome shotgun sequence.
ACCESSION   DAORQA010000192 DAORQA010000000
VERSION     DAORQA010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6496_length_3494_cov_6.617621"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1198)
                     /locus_tag="PK736_07970"
     CDS             complement(170..1198)
                     /locus_tag="PK736_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRC33365.1"
                     /translation="MDNILIIYTGGTIGMVKDAITGSLKPFDFGEIYARIPELHAFDC
                     HIDFITLEPLIDSSDVTIDNWVEMALLIKKNYARYKAFIILHGTDTMAYTASALSFIL
                     EGLNKPVILTGSQLPIGAVRTDARLNLITTIEMAYQCIKKNLGLSEVCICFDDVLMRG
                     NRCEKYSSSKFYAFKSENYPPLAEAGTEIVFHQQNFLPQPKIKLRIQEQLCNDIALLK
                     IYPGMPNSLLDSYLNNKKLKAIVIETFGAGNAPVNKSFINFIEKAIANKLLVVNVSQC
                     IGGMVEPGKYAGSYRLDKTGVLNGSDMTTESAITKLMYLFGKNLSLQTIKKNMTISLR
                     GEMSVSPT"
     gene            complement(1268..2896)
                     /locus_tag="PK736_07975"
     CDS             complement(1268..2896)
                     /locus_tag="PK736_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33366.1"
                     /translation="MSYYDRPAGTMTYHPTHQHMHVDDWGIYTLRIATLDPNPLNWPI
                     VGTGAKLAFCLMDLSTCTAEFGNCVNDKNDTLVNTDFPNYGLGGGQYTCSPVVQGISS
                     GFVDYYDQTLDGMWINIPPGTCNGDYWIVTQIDPYNYFDEENENNNIVATPITLKKQT
                     PFGTGGIIANLGAQTICNGGTTTLQANLGTTYLWSNGATTQTIVVSASGTYMVTVDGF
                     CGVTTSPPYTVSMITPAVPTAQNSYFIAPASTTVSATGTGKIDWYDASSGGNFLGTGN
                     VFTTGVLNNTTTFYAENIETQIGLTAYAPPSANTIGGGANHTDQTRFLTFDAYKDFML
                     QSVKVYATGAGNRLIELRDGGGGILDSVTAMVPSGESRITLNFQVAKGSNYQLGVGST
                     PNFFRNNSGVQFPYNVSGMLDITSSSAGASYYYFFYDWEVKENDIVCNSSRVPATAFN
                     STTGITNNINSVVWNTYPNPASGYLTVELPSLPENSKLELIDVTGKKVWEQNIGSTNK
                     PSIISIDVTKMSAGTYSLKIENEKISERKILIIE"
     gene            complement(2883..3287)
                     /locus_tag="PK736_07980"
     CDS             complement(2883..3287)
                     /locus_tag="PK736_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33367.1"
                     /translation="MKKLICLIFLIISTFSSYSQCNTGNATTCVCADGSIKCKLLPDI
                     TIGRKMLMDPNTYTEYSQTGNGVENGRLRISVATPNIGHGPLTVMTTTTFVCGTDTFY
                     GTSPGTCPNGDAPKQLINQRVFSKKRKNHVLL"
BASE COUNT         1015 a          654 c          673 g         1152 t
ORIGIN      
        1 gttgattttt acctgtgact atgtcgcgaa tatagaacag caaaatattc cgataataaa
       61 tagagatttt ttaaacattg tattcttctt aaaggaatta caaaagtacc aataattgct
      121 ttggtagaaa tatatatttt aaatgagtta accctttatt aaccaatcgc taagttggag
      181 atacgctcat ttcgcctcgc aaagaaattg tcatgttttt tttaatggtt tgcaaagaca
      241 ggtttttccc gaataagtac atcagttttg tgattgccga ttctgttgtc atatcacttc
      301 catttagcac ccctgtttta tcgagtctat aacttccggc atattttccc ggttccacca
      361 tgcctccaat acattgcgaa acatttacaa caagcagttt attggcgatg gctttttcta
      421 taaaattaat gaatgacttg ttaaccggag cattacctgc accaaatgtt tctattacaa
      481 tggctttaag ttttttatta ttcaaataag agtccagtaa cgaattgggc atgccgggat
      541 aaattttcag caatgcgatg tcattgcata actgctcctg tatacggagt ttgattttgg
      601 gttgtggtaa aaaattttgc tgatgaaaaa caatttctgt accggcttcg gcaagcggag
      661 gatagttttc tgatttgaag gcataaaact ttgaagatga atatttttcg cacctgttgc
      721 cacgcattag cacatcatca aagcatatac acacttcact caaaccaagg tttttcttta
      781 tgcattgata agccatttct atggttgtaa taaggtttag ccttgcatcg gttcgcactg
      841 cacctattgg caattgcgac cctgtaagta ttaccggctt atttaaccct tccaaaataa
      901 aactcaaagc cgatgcagtg taagccatgg tgtctgttcc atgtaaaatg atgaacgcct
      961 tgtaacgggc atagtttttt ttgatgagca aagccatttc aacccagttg tcaattgtta
     1021 catcactgga gtctattaat ggctcaagtg tgatgaaatc gatatgacaa tcaaatgcat
     1081 gcaattcggg aatgcgggca tatatttcac caaaatcgaa tggtttcaaa ctacctgtta
     1141 tagcgtcttt caccatgcca atggtaccac cggtgtatat gattaaaata ttatccatta
     1201 aatgatgaaa taaaaaaagg ctgcacaaat atataatgtg cagcctttat tatcgtgtct
     1261 tattttttta ttctattata agaattttac gctcgcttat cttttcgttt tctattttca
     1321 acgaataggt tccggcactc atttttgtta catcaatact tattatagat ggtttgttcg
     1381 ttgagcctat gttttgttcc caaacttttt tcccggttac atcaattaat tctagttttg
     1441 aattttcggg taatgatggt aattcaactg ttaaatatcc acttgcagga ttagggtaag
     1501 tattccaaac aacgctgttg atgttattgg tgattccagt tgtggaatta aatgccgtag
     1561 ctggtactct cgaactattg caaacaatat cgttctcttt tacttcccaa tcgtaaaaga
     1621 aataatagta tgaagcacca gccgaagagc tagtaatatc aagcattcca cttacattat
     1681 acggaaattg cacaccggaa ttattacgga agaagtttgg ggtggaacct acgcctaatt
     1741 ggtaattgct accttttgca acttgaaaat ttagtgtgat ccggctttca ccagaaggca
     1801 ccatagctgt tacagagtca agaatgccac caccaccatc gcgtaattct attaaacgat
     1861 tgccggcacc cgttgcatat acttttactg attgtagcat aaaatcttta taagcatcaa
     1921 aagtgaggaa tcgtgtttgg tcggtgtggt ttgctcctcc tccaatagtg tttgcagatg
     1981 gtggtgcata agcagtaagg ccaatctggg tttcgatatt ttcagcataa aatgttgtag
     2041 tattgtttag cactccggtt gtaaacacat tacctgtacc taaaaaatta ccacctgacg
     2101 aagcatcata ccaatcaatc ttgcctgttc ctgtagcact aaccgttgtg ctggcaggag
     2161 caatgaagta agagttttgt gcagtaggta cagcaggggt tatcatgctt actgtgtatg
     2221 ggggtgatgt ggtaacacca caaaaaccat caacggttac catatacgtt ccagaggcag
     2281 aaactacaat tgtttgtgtt gtagcaccat tactccacaa gtatgtagta cctaaattag
     2341 cttgcagagt ggtagtgcct ccgttacaaa ttgtttgtgc acccagattg gcaatgattc
     2401 cgcctgttcc aaacggtgtt tgctttttta gtgtaatggg tgtagccact atattgttgt
     2461 tttcattttc ttcgtcaaaa taattataag gatcaatttg ggttactatc caatagtcgc
     2521 cattgcatgt acctggaggt atattaatcc acatgccatc taaagtttga tcgtaataat
     2581 caacaaatcc cgatgaaatt ccctgaacta caggcgaaca ggtatactga ccaccgccta
     2641 agccataatt ggggaaatct gtgttaacta atgtgtcatt tttatcattc acacaatttc
     2701 caaattctgc ggtgcaagta cttaaatcca tcaggcaaaa tgcaagctta gcaccagtgc
     2761 caactattgg ccagttgagt ggattggggt ccaatgtggc tattcgtaaa gtataaattc
     2821 cccagtcatc aacatgcata tgctgatgtg tgggatggta tgtcattgta ccggccggcc
     2881 gatcataata agacatggtt tttccttttt tttgagaaca ctctctgatt aatcaattgt
     2941 tttggggcat caccatttgg gcaagttcca ggcgatgtgc cataaaaagt gtcggtacca
     3001 caaacaaaag tagtggttgt cattactgtt aatggtccgt ggcctatatt tggcgtggca
     3061 acagaaatac ggagacggcc attttcaact ccattacctg tttgcgaata ttcagtatag
     3121 gtgttgggat ccataagcat ttttcgtcca atagtaatgt cgggaagtag tttacatttt
     3181 atggaaccat cagcacatac gcatgttgtg gcattgcctg tgttgcattg tgagtatgaa
     3241 ctaaaagttg aaatgattaa gaaaataagg cagatcagtt ttttcataat ttgaaatatt
     3301 tggtttgcga aatataaaaa caaaacttaa taaaaaaacc cgctctatag aagcgggttt
     3361 aaattatatt gtaactaatt gaacaacaat ggtttaagtt ttagccgatt tgcacgcgtt
     3421 tcattgcgct tattgttaag cctttgtcaa ttgactccag atattgtctt actgtttgct
     3481 tgtcagcctt tatg
//
LOCUS       DAORQA010000193         3481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6521_length_3481_cov_9.405721, whole genome shotgun sequence.
ACCESSION   DAORQA010000193 DAORQA010000000
VERSION     DAORQA010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6521_length_3481_cov_9.405721"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1884)
                     /locus_tag="PK736_07985"
     CDS             complement(<1..1884)
                     /locus_tag="PK736_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33368.1"
                     /translation="MKKIIFAFFISLTFFACRKENGNPSWETTNAVPLLRSSLSIDNL
                     VTDSLLNVDASGAISLVYQNHLYDFRLDSLFSLFDTSITRNYKLDSLSLFSQSLTYPI
                     SLGAIAQNAGLLGYAIIINNGNTAAIPAFPNVSTGAQPINVDSLFQTITLVTGFMDIT
                     LTNGLPIDITNLAFELRNQSNNSLIAVDTFPIIAAGATETRTISMAGKTIEGKMNASL
                     VLNSPGSGGNPVLIDTSDAIIAQLKVYDMHPLTATAIWPAQDLVNKAQPFKLKVEKVQ
                     LTEARLQSGYIVVDLYSTLQDSVRFDYSLPLAYFNGVPFYLQTILPPAPPGGTSHFYQ
                     QFDFTGYQLDLTGINHDTVNTMWQNIRARIDSTGIMKTIALTDSFYANIGFIDLVPEY
                     AKGYLGQDTFDINPTTVNLDIFNNVNADVFDLKDVKLNLKVNNGIGIDGRLDLKQLTS
                     YNTKKNKTVNLSGAALNNPFLINRAIDPNGWPGPVTYSPTQLSLNDNNSNVSAFISNL
                     PDKITTAATLYTNPQGNVTNYNDFIYYNDGIKLDLDFEMPLNFMADNLSLCDTVVASI
                     NKADISKIKSAVISLFADNGMPLDATLSIYMLNSNNQIIDSLLHQLNRSTVGATIYNK
                     YTLE"
     gene            complement(2025..>3481)
                     /locus_tag="PK736_07990"
     CDS             complement(2025..>3481)
                     /locus_tag="PK736_07990"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRC33369.1"
                     /translation="RGDHGFKQSFTFVKGRELGPKNALTIDKISYTVEKDFTPQPFYF
                     DTSVTGTLVNVGYGIAADSLGWNDYTKLTNLKGKIFLMDLFSPDGNNPHGKYSGFADM
                     ETKVLTALEKGAAGIIFYDKNGDGPAPEVDLNTKRNKQLIPVIVVKGELNKKIKKLVG
                     RNVTMQIDLQKIVSAGCNVIGYIDNNAAQTVVLSAHYDHLGMGISGSLHRGEQAIHNG
                     ADDNASGTAAIIELARLLINSDLKNNNYMFCAFSGEELGLLGSNYFVKNPTIMLDKLN
                     YNINLDMVGRLKAEEPVLLVNGVGTSPQWMPAMNDIHSLKIKTTESGVAPSDNTSFYL
                     NGVPAIHMFSGSHSDYHKPSDDEALINYPGEVVIIQYIFELIKNLNDDGKLTFAKTND
                     ANSDNAPRFKVTLGVVPDYAYEGEGMRIDGITDGRPAAKAGLQKGDIVIQIGEEKVYD
                     MTSYMKALSKFKKGETTIVKVKRGETLIDSNLTF"
BASE COUNT          984 a          680 c          712 g         1105 t
ORIGIN      
        1 ctcaagcgtg tatttattgt aaatggttgc acccaccgta ctgcggttca attggtgcaa
       61 cagcgaatcg attatttgat tattggaatt gagcatataa atagaaagtg tggcatcgag
      121 tggcatacca ttatcggcaa acaaacttat cacggcactt tttattttgc ttatatcggc
      181 tttgttgatt gatgcaacca cagtgtcgca caacgaaaga ttatcggcca taaagtttag
      241 cggcatttcg aaatcgaggt cgagttttat accatcatta taataaataa aatcgttata
      301 gtttgtcaca ttgccttgcg gattggtgta aagcgtagca gcagttgtaa tcttgtcggg
      361 caggttcgat ataaatgccg acacattgga gttgttatca tttaacgata attgtgttgg
      421 cgaataagta acagggccgg gccagccatt cgggtctatg gcacgattta tcaggaaagg
      481 attgttgagt gctgcaccac ttagatttac agttttgttt ttcttggtgt tgtacgatgt
      541 caattgtttt aaatctaacc gaccatcaat tccaattccg ttattcactt tcaggttaag
      601 ttttacatct ttcaaatcga acacatcggc attcacgtta ttaaaaatat cgaggtttac
      661 tgtagtagga tttatgtcga aagtatcttg tcccaaataa ccttttgcat actcgggcac
      721 caaatcgata aagccaatat tggcataaaa cgaatcggtg agtgcaatgg ttttcattat
      781 acctgtagag tcaattcgcg cacgaatatt ttgccacatg gtatttactg tatcgtgatt
      841 gatgccggtt aaatcgagct gatagccggt aaaatcgaat tgctgataaa agtgtgatgt
      901 gccacccgga ggggcaggag gtaaaatggt ttgaaggtaa aaaggaacac cattaaaata
      961 tgcaagtggc aacgagtaat cgaaacgcac actgtcttgc aaagtgctgt ataaatcaac
     1021 tacaatataa cccgattgca aacgcgcttc ggtaagttgt actttttcca ctttcagttt
     1081 aaatggttgt gctttgttta ctaaatcttg tgcaggccaa attgctgttg ctgttagcgg
     1141 atgcatatcg tacactttca actgtgcaat gatggcatcg ctggtgtcaa tcaacaccgg
     1201 attgccacca ctgccgggcg aattaagcac aagcgaggca ttcatttttc cttctatggt
     1261 ttttcctgcc atcgatattg ttcgcgtttc ggtagcacct gcagctatta tcggaaatgt
     1321 gtcaacagca ataagagagt tgttcgattg attgcgcaac tcaaaagcta agttcgtaat
     1381 gtcgataggc aatccgtttg taagtgtaat atccataaat cctgtaacga gggttatggt
     1441 ttgaaacaag ctgtcgacat ttataggttg tgcaccggta gacacattag ggaatgccgg
     1501 aatagctgcc gtgttgccat tgttgatgat aatggcataa ccaagcaggc cggcattttg
     1561 tgctatggcg ccaagcgaaa tgggatacgt taatgactgc gaaaaaaggc tgagcgaatc
     1621 gagtttataa ttccgcgtaa tgctggtatc aaataaacta aagagcgaat cgaggcgaaa
     1681 gtcatataaa tgattttgat acacaaggct gatagcaccg cttgcatcta catttaataa
     1741 cgaatcggta acaaggttgt caattgacaa actcgaccgt agcaaaggca cagcgttggt
     1801 ggtttcccac gatggatttc cattttcttt gcggcaggca aaaaaagtta gtgaaataaa
     1861 aaacgcaaaa attatttttt tcattgtgtg atattttatc atgctatttt tgaaaggctg
     1921 cagtgaaaac cagtaccaga attttatttt ttcctattaa tctatagtac gcactttaag
     1981 tactttatgt gagttttaat ttaattgtgt gcaactgcaa ttttttaaaa cgtaagatta
     2041 ctatcaatca aagtttcgcc acgttttact tttacaatgg tggtttcacc ttttttaaat
     2101 ttcgataagg ctttcatgta cgatgtcatg tcatatactt tttcttcgcc aatttgaatt
     2161 actatatcgc ctttttgtaa tccggctttt gcagcagggc ggccgtcagt aataccatct
     2221 atgcgcatac cttcaccttc gtatgcatag tcgggcacaa caccaagtgt aaccttgaat
     2281 cgaggcgcat tatcactatt ggcatcgttt gttttcgcaa atgtcagctt gccatcatcg
     2341 ttaaggtttt ttatcagttc gaaaatgtat tggataatga caacctctcc gggataattt
     2401 atcagtgcct catcgtcact tggcttgtgg taatcgctat gtgagccact aaacatgtga
     2461 atggccggca ctccattcaa atagaatgaa gtattatcgc tgggcgccac accactctca
     2521 gttgttttta tttttaatga atgaatatcg ttcattgccg gcatccattg tggtgatgta
     2581 cctacaccat ttacaagtaa tacaggttct tcagctttta atcggcctac catatcgagg
     2641 ttaatattat aattaagttt atcgagcatg atggtaggat tttttacaaa ataatttgag
     2701 ccaagcaacc ccagctcttc gccactgaac gcacaaaaca tgtagttgtt atttttcaag
     2761 tcgctattga ttagcaagcg ggctagttcg ataatggctg cagtaccact ggcattgtca
     2821 tcggcaccat tgtgtattgc ctgttcgcca cgatggagtg agcccgaaat tcccattccc
     2881 aaatggtcgt aatgtgcgct cagcacaacg gtttgtgcgg cattgttatc aatatatcca
     2941 atcacgttgc aaccagccga tactattttt tgcaaatcga tttgcatggt aacatttcgg
     3001 ccgacaagtt tttttatctt tttatttaat tcacctttta ctacaattac cggaatcaat
     3061 tgcttattgc gttttgtatt gaggtcaact tcgggtgcgg gaccatcgcc atttttatcg
     3121 taaaaaataa ttcctgcagc acctttctct aaagccgtta acaccttggt ttccatatcg
     3181 gcaaagcccg aatatttacc atgcggattg ttgccatcgg gcgaaaacaa atccatcaaa
     3241 aatatttttc cttttaaatt tgtgagctta gtataatcgt tccacccaag cgaatcggca
     3301 gcaatgccat atccaacatt tactaaagtg ccggtaacac ttgtatcgaa ataaaaaggt
     3361 tgtggcgtaa aatctttctc aacggtgtaa gaaattttat cgattgtgag agcatttttt
     3421 ggtcccaatt cgcggccttt aacaaaagta aaagattgtt taaatccatg gtcgccacgc
     3481 t
//
LOCUS       DAORQA010000194         3431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6622_length_3431_cov_11.042950, whole genome shotgun sequence.
ACCESSION   DAORQA010000194 DAORQA010000000
VERSION     DAORQA010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6622_length_3431_cov_11.042950"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..679
                     /locus_tag="PK736_07995"
     CDS             425..679
                     /locus_tag="PK736_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33370.1"
                     /translation="MYIFTTKTNHTMEEVMGAKCNPMLFNFVFVKSEDKLLEIKKLAT
                     ENKLTSTVFVFFSNGKAIRYCNVEEFVKAVENKVELRIEE"
     gene            881..1966
                     /gene="rfbB"
                     /locus_tag="PK736_08000"
     CDS             881..1966
                     /gene="rfbB"
                     /locus_tag="PK736_08000"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRC33371.1"
                     /translation="MQNKQSILITGGAGFIGSHVVRRFVNHFTDVMIYNLDKLTYAGN
                     LENLKDIEHAANYKFIKGDITDTTFINKIFAEYKFDAVIHLAAESHVDRSITSPMDFV
                     MTNIVGTVNLLNAAKTLWSNNPIDDKNTNSVRRIFYHISTDEVYGTLGSTGHFTEQTP
                     YDPHSPYSASKAGSDHLVRAYHDTYKMPVVISNCSNNYGSHHFPEKLIPLAINNIKNN
                     KPVPVYGAGLNVRDWLWVNDHASAIETIFTKGVIGETYNIGGNNEWKNIDLIHLLCEI
                     LDTKLNREKGESAKLITFVKDRAGHDLRYAIDATKLKNELGWSPSITFEEGLALTVDW
                     YLHNEEWLTHVTSGEYLKYYQGQYEQR"
     gene            1994..2995
                     /locus_tag="PK736_08005"
     CDS             1994..2995
                     /locus_tag="PK736_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC33372.1"
                     /translation="MSSIYTEVFHTPELKNKNILVTGGAGFIGSNIVEYLLQHGVKKV
                     RVLDNLSNGFMKNVDIFKSNPNYEFINGDITNYDDCVRACDGIDIITHQAALGSVPRS
                     IQFPLATHAANATGFINMINAARENNVARFVYASSSSVYGDSLASPKHEKDLGTPLSP
                     YAVSKLTNELYGNIFGLTYKIEVVGLRYFNVFGPRQDPNGPYAAAIPIFMNGLLNNTD
                     VYINGDGSNTRDFTFVVNAVQANICAMCTTHNDAFNKVYNIAFGSATSLNELYQILQK
                     VSQNNMPANYRDERPGDIKNSLADISLAKKYLSYNPKVAIDEGLKITYNWFKETFGK"
     gene            3054..>3431
                     /locus_tag="PK736_08010"
     CDS             3054..>3431
                     /locus_tag="PK736_08010"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HRC33373.1"
                     /translation="MGFFTNIFKQKEPPALLDFSSIKTDIHSHLIPGIDDGAKTMDES
                     IELIKVQYELGIRNFVTTPHIMSDFFKNTPEIILGGLNELRIVLKQENIDVTINAAAE
                     YYLDEGFVHKLDHEKLLTIGDNYL"
BASE COUNT         1148 a          618 c          653 g         1012 t
ORIGIN      
        1 tacaaaagaa aaaacagaaa tagatttgcg aaaaataaat aatggaatgt actatgtgat
       61 ggtgtatgat ggaaagaata tatttacacg gaaaataata aaggaatgaa aaaaatacgg
      121 ccagcgctca atcgtaaccc tgaaggatgc caaagtatcg tataaaagaa aattttgtta
      181 gtacttatgg ctgactacct ttaggcatgc cccggcctta gtgtcggtgg gttcggggtg
      241 caaagtgccg aatgaaactt aaaattctta aatcaaaata aaacaaaaaa atatgatgaa
      301 cacttttaaa ttccacacag cggggaaaaa cctcctcctc ttaattctcc tttttgtatc
      361 ctctttggcg atacaaaaag taaatgcaca agcatgcaac ttgccaatta tgaatatgca
      421 cacagtgtat attttcacaa caaaaacaaa ccacactatg gaagaagtga tgggtgcaaa
      481 atgtaatcct atgcttttta attttgtgtt tgtaaaatcg gaagacaaac ttttggaaat
      541 aaaaaaactg gcaacagaaa ataaattgac ctcaacagtg ttcgtttttt ttagtaacgg
      601 taaggctata cgttactgca atgtggagga gtttgtaaag gctgtagaga ataaagttga
      661 attgaggata gaggaataga gttttgagtt cctagttttt agttctatgt tctttgtctt
      721 ttgcttcagt aaaaactcct tttcataaaa atttgttgcc atgaattata ataaatcaat
      781 gagccacaag catggtcaat tactgtagca ttggctaact gaattaaatt ttttgtttgc
      841 ataactttca gtttatttgt gcattactca aaaaaaataa atgcaaaaca aacaatcaat
      901 attgataaca ggaggtgccg gatttattgg cagccatgtt gtacgcaggt ttgtaaatca
      961 ctttacagat gtgatgattt acaatctcga taaacttact tatgcgggaa atctcgaaaa
     1021 cctgaaagat atagaacatg ccgcaaacta taagtttata aaaggtgata ttaccgatac
     1081 aacttttatc aataaaattt ttgcagaata taaatttgac gcagtaatac accttgctgc
     1141 cgaatcgcat gttgaccgaa gcattacaag ccccatggat tttgtgatga caaatattgt
     1201 tggcaccgtg aatttattga acgcagcaaa aactttgtgg agcaacaatc ccatcgatga
     1261 taaaaataca aattctgttc gcagaatatt ttatcacatt tcaaccgatg aagtatatgg
     1321 cacactgggc agtacaggac attttaccga gcaaactcct tacgaccctc acagtcctta
     1381 ttcggcatcg aaggcgggca gcgaccacct tgtacgggct tatcacgata catacaaaat
     1441 gcctgtggtg attagtaact gtagcaataa ttatgggtcg catcactttc ccgaaaaatt
     1501 aattcctctt gccattaaca atattaaaaa taataaaccc gtacctgtat atggtgctgg
     1561 actcaatgtt cgcgattggt tgtgggtaaa cgatcacgct tcggccatcg aaactatttt
     1621 tacaaaaggc gtgataggtg aaacttataa tattggtggc aacaacgaat ggaaaaacat
     1681 agacctcatt catttgctgt gcgaaatttt ggacacaaaa cttaatcgtg aaaagggaga
     1741 atcggctaaa cttatcacat ttgtaaaaga ccgcgcaggt cacgatttgc gttatgctat
     1801 cgatgctaca aaactcaaaa acgaactggg gtggtcgcct tccattacgt tcgaagaagg
     1861 gctggcactc actgtggact ggtatttgca taacgaagaa tggctcacac atgttacttc
     1921 gggtgagtat ttaaaatact accagggcca gtacgagcaa cgataatttt atttcgcaaa
     1981 acagtattca cttatgtcat ccatttatac agaagtcttt catacacctg aactgaaaaa
     2041 taaaaacatt ttagttacag gtggtgcagg ttttataggc tccaatattg tagaatatct
     2101 tttgcaacac ggagtaaaaa aagtgcgcgt gctcgacaat ctttccaatg gttttatgaa
     2161 aaatgttgat atttttaaat cgaacccaaa ctacgaattt ataaatggcg atattacaaa
     2221 ctatgatgat tgtgtgcggg catgcgatgg aatcgatatt ataacacatc aggctgcatt
     2281 aggttcggtg ccgcgttcca tacaatttcc attggctaca catgcagcaa atgcaacggg
     2341 ttttataaat atgataaatg ccgcccgcga aaataatgtt gcccgctttg tgtatgcaag
     2401 ttcctcttcg gtgtatggcg acagtttggc atcgcccaag cacgaaaaag atttaggcac
     2461 tccactttcg ccttatgcag tttcgaaatt gacaaatgaa ctgtatggaa atatttttgg
     2521 cctcacttat aaaatagaag tagtggggtt gcgctatttt aatgtgtttg gcccaagaca
     2581 agaccctaat ggtccttatg cagctgccat accaattttt atgaatggct tgctcaacaa
     2641 cacagacgta tacataaacg gtgatggcag caacactcgc gactttactt ttgtagtcaa
     2701 cgctgtgcag gcaaatattt gtgccatgtg tacaacacac aatgatgcat ttaataaagt
     2761 gtacaacatt gcctttggta gtgccacttc actgaatgag ttataccaaa ttttgcaaaa
     2821 ggtgtcgcaa aataatatgc cggcaaacta tcgagacgaa cgtcccggtg acataaaaaa
     2881 ttcgctggcc gatatttctt tggctaagaa atacttatca tataacccga aagttgccat
     2941 agacgaaggt ttgaagatta catacaactg gtttaaagaa acttttggta aatgatttta
     3001 tggtttgttt aaatctgtaa attcgccaac tctcttctac caaaaattat caagtgggat
     3061 tttttacaaa tattttcaaa caaaaagaac ctccggcatt gttggatttt tcttccatca
     3121 aaacggacat acattcacat ttgattccgg gcatcgatga tggtgcaaaa acaatggatg
     3181 aatctattga gcttataaag gttcaatacg aattgggcat ccgcaatttt gtaaccacac
     3241 ctcatatcat gagcgatttt tttaaaaaca ctcccgaaat tattcttggt ggattgaatg
     3301 aattgcgaat tgttcttaag caagaaaata tcgatgtaac tataaatgct gctgctgaat
     3361 attatctcga tgaaggtttt gtacataaac tcgaccacga aaaattgctc accattggtg
     3421 acaattattt a
//
LOCUS       DAORQA010000195         3416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6662_length_3416_cov_5.612913, whole genome shotgun sequence.
ACCESSION   DAORQA010000195 DAORQA010000000
VERSION     DAORQA010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6662_length_3416_cov_5.612913"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..602)
                     /locus_tag="PK736_08015"
     CDS             complement(234..602)
                     /locus_tag="PK736_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33374.1"
                     /translation="MWGKHQPQACQMLGENTQGIYVVGRDRRMRVVGENTNHRHRVVG
                     ENTNHRHRVVGENTNHRHTPQAQWWFVGTQTTEEEENSSHRQPKISRIFAMVFEHMLQ
                     YICTCLFVVWIKKLKFEIID"
     gene            886..1116
                     /locus_tag="PK736_08020"
     CDS             886..1116
                     /locus_tag="PK736_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33375.1"
                     /translation="MKFLVDDKRVWIYGFVIMPNHLHVLWRMQESFEEKNVQRDFLKF
                     TAQKIKFDLLENHPQVLPHFKSTQNDREYQFW"
     gene            complement(1684..2331)
                     /locus_tag="PK736_08025"
     CDS             complement(1684..2331)
                     /locus_tag="PK736_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC33376.1"
                     /translation="MPEKIRVLIFEDHAQMRNMYELMLDSEPDMLCVGAQENCNNAIK
                     IIEKTLPDVILMDIQMPGKSGVEAAREIKNIFPTINIIMQTIFSDDDKIFESICNGAS
                     GYLLKNSTLEEFLSAIRQVHVGGAPMSPSVALKVLNRFRQSNTAVLPPENFNLTDREK
                     EILGLLANGQSYKLIASQLNISYYTVDSHIRKIYEKLHVRSATEAIKKAIDNRLV"
     gene            complement(2393..>3416)
                     /locus_tag="PK736_08030"
     CDS             complement(2393..>3416)
                     /locus_tag="PK736_08030"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HRC33377.1"
                     /translation="FDTDADSLLAQAKPGIHQQYNSLTFTFSYLDYVNGGTIKYLYKV
                     AGMNDEWIDNGNSNKINITNLKSGDYTLIVRAAYRDANEPAGEISYTFKVIPYWYLTW
                     WFYFLCFAVAGGGGYLLYMYRLKQVRKFYEMRNNIARDLHDDVGAALSSILIGTQLME
                     GLVPRENEKQHKIIDAMKSASSQTISNMSDIVWSLNPKNDTGLMMIEKMKKTIFELLE
                     QKQIKVEFIYAPEFENVVIPMDVRKNIFLIFKEAIHNISKYAYAREVRIDIAVDSKKL
                     QLKISDNGKGFDNKYIQAGNGLENMKRRATEINAALEIDSAPNKGTKIILQCGITNIR
                     DIKNQK"
BASE COUNT         1003 a          661 c          627 g         1125 t
ORIGIN      
        1 gtttttactt gttcgctcca tggctgcgca aacaaaagtt tactgcaaat tataaacgca
       61 aaagttagta gatgtttttt cataatgctt tttaattaat tagtactcga atttatattg
      121 acaaagaaac aatgttaaag aaagagcata aatactcaat ttgtagtagg ttgcaaagtc
      181 agttatcgta tcaaactatt atctataaat gattatttga aaaattattt tcttcaatca
      241 attatttcaa acttcaattt tttgatccaa accacaaaaa gacatgtaca aatgtattgc
      301 aacatatgtt cgaaaaccat agcgaatatt cgggatattt ttggttgacg gtggcttgag
      361 ttttcctctt cctccgtggt ttgtgtcccc acaaaccacc attgtgcctg tggcgtatgc
      421 ctgtggttgg tgttttcacc aaccacccta tgcctgtggt tggtgttttc accaaccacc
      481 ctatgcctgt ggttggtgtt ttcaccaacc accctcatcc tcctgtccct gccaaccaca
      541 tatatgccct gcgtattttc gccaagcatc tgacatgcct gtggttggtg ttttccccac
      601 accacctata tgcctatatg ctctgcgaac tttcaccaac tacctcgaca tgcccgtggt
      661 tggtgttttc accaaccacc catgcccgtg gttggtgttt tcaccaacca cctagatgac
      721 tcgaattggt attttcacca accacatttt gatataatcc caaaacattt ctatttttcg
      781 ttttaaaaaa aaatgaaaaa tgaaataaaa ttttttaccg caactatatt atattggaaa
      841 aaacttttgt accccgacaa atacaaacaa attattatta acagtatgaa atttttagtt
      901 gatgataaac gcgtttggat atatggtttt gttattatgc caaatcattt acatgtactt
      961 tggaggatgc aagagtcctt tgaagaaaaa aatgttcaac gagattttct aaaatttaca
     1021 gctcaaaaaa ttaaatttga tttactcgaa aatcaccctc aggtattgcc acattttaaa
     1081 tcaacccaaa acgaccgcga ataccaattt tggtaacgca gagcatattc atcaaccata
     1141 tacaatagat tagttttcga gcagaaatta aattatatcc ataacaaccc tcttgtagga
     1201 aaatggaaac ttgccgaaag tgctgaacta tatttttatt cgagcgctaa atattatttg
     1261 gcaggcaatt cgaatttcag ttttcttact cactatactg agcatatatg ataactgatt
     1321 ttgtggttga tgatggagga ttggctggta atggagggtt tggtggttgg tgaaaacacc
     1381 aaccacaggc atgcgagcac aacgatgatg agagaattgg tggttggtga tgggaggata
     1441 tggtggttgg tgaaaacacc aaccacagac acgagagcac atcctccatg gtttttgtcc
     1501 ccacaaacca ccatggcatt caccatagtt tatttccccc aaattacggc aattaagagg
     1561 atggtttgtg gggacacaaa ccatggggga aggagatgaa atggtttgtg tttcaatcaa
     1621 ccatggggga gggagatgaa atggtttgtg gggaaacaaa ccatggagta cggatgcagc
     1681 caactaaact aacctgttat caatggcttt tttaatagct tcggtagcgg agcgcacgtg
     1741 gagtttttcg tatattttgc ggatgtgaga atcaactgtg tagtaactta tgtttaattg
     1801 cgatgctatt aatttatagc tctggccgtt ggcaagtaaa ccaagaattt ctttttcgcg
     1861 atcggtgagg ttgaagtttt cgggtggcaa tactgctgtg tttgattgtc tgaatctgtt
     1921 gagtacttta agtgccacac ttggcgacat aggtgcaccg ccaacgtgta cctgccgtat
     1981 tgccgacaaa aattcttcga gtgtgctgtt ctttaacaga taacccgaag caccgttgca
     2041 aattgattca aagattttat cgtcatcgct aaaaatagtt tgcataatga tgttgatagt
     2101 ggggaaaata tttttgattt ctctcgcagc ctctactccc gatttgccgg gcatttgtat
     2161 atccatcaat ataacatcgg gcaaagtttt ttcaattatt ttaatagcgt tgttgcaatt
     2221 ttcctgtgca cctacacata gcatatcggg ctcactgtca agcatcagct catacatatt
     2281 acgcatttgc gcatggtctt caaaaatgag tacacgtatt ttttcaggca tttcaaatta
     2341 taattataag ttttgtttaa gaaggaatag agataaagca caaactacta cactattttt
     2401 ggtttttaat atcgcgaata ttcgtgatgc cacattgcaa aataattttt gtccctttgt
     2461 ttggtgcact gtcaatttca agtgctgcat ttatttctgt cgctctacgt ttcatatttt
     2521 cgagcccatt gcctgcttgt atatacttat tatcgaaacc ttttccgtta tcgcttattt
     2581 ttagttgcaa cttttttgag tcaacagcaa tatcaattct cacttcacgg gcatatgcat
     2641 atttcgaaat attatgaata gcctctttaa aaataagaaa aatatttttc cgcacatcca
     2701 tgggtatcac aacgttctca aactccggag cataaataaa ttcaacctta atttgttttt
     2761 gttcaagcaa ttcgaaaatg gtttttttca ttttttctat catcatcaag cctgtgtcat
     2821 ttttgggatt gagcgaccaa acgatatcac tcatattgga aatagtttgc gatgatgcgc
     2881 ttttcatggc atcaataatt ttgtgttgtt tctcgttttc acgaggaacc aagccttcca
     2941 taagttgagt gccaattaaa atactggaga gtgcggcacc aacatcatcg tgcaaatcgc
     3001 gggcaatatt atttcgcatt tcataaaatt tgcgcacttg ttttaaacga tacatataaa
     3061 gtaaatatcc tcctccacca gcaactgcaa aacacaaaaa ataaaaccac catgtaaggt
     3121 accagtatgg aattactttg aaagtgtagg atatttctcc ggccggctca tttgcatcgc
     3181 gatatgctgc acgcacaatt aaagtataat cgccactttt taaattcgta atatttattt
     3241 tgttgctgtt tccgttatct atccattcat cattcatgcc ggctactttg tacagatatt
     3301 ttatagtgcc accgttaaca taatcgagat acgaaaacgt aaacgttaag ctgttgtatt
     3361 gttgatgaat gccgggtttg gcttgcgcta ataaagaatc ggcatcagtg tcgaat
//
LOCUS       DAORQA010000196         3328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6872_length_3328_cov_8.549649, whole genome shotgun sequence.
ACCESSION   DAORQA010000196 DAORQA010000000
VERSION     DAORQA010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6872_length_3328_cov_8.549649"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3229
                     /locus_tag="PK736_08035"
     CDS             <1..3229
                     /locus_tag="PK736_08035"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRC33378.1"
                     /translation="IYSMNNALKTSYCKYRIYVISLILFNFIGINYVYSQTYPSGFTQ
                     VAVASGLSSPTNFAFAPDGRIFICEKAGKIKILKNGILLSTPFLSLNVNNAGERGLLG
                     IAFDPNFTTNKYVYVYYTISAATNNRVSRFTASGDIAISNSEVIVRTMDALSSATNHN
                     GGTIKFGPDGKLYVAVGENTNSTASQSLTTTMGKMLRMNSDGTPAVGNPFYSSTNAQK
                     KLIWCYGLRNPYSFDFDSNTGKLFINDVGSNSSEEINDGTNGNLNFGWPNVEGISGNA
                     AYTDPVFAYAHGVTNTTGCAITGGAFFNSSISNYPAAYANKYFYIDYCNQWISYINVS
                     SQSNSTVFATGISDEMVCIDQGKDGNLYFLSRIANKLYKISYNNITVPTIISQPVGLS
                     VSLGQPASFSVVASGSTPLTYVWKKNGVSIANSNSAIYTINKVTSNSAGTYSCYITNS
                     FGSVTSTGAVLVVTAFNAAPVATIITPTNNTLYSGGMTVNYSGTATDAEDGNIASANF
                     LWEIRFFHDTHNHPGPLAVAGAKSGSFNIPNVGETSENVFYRIYLYVKDSKGLYDTAF
                     VDLIPKTSIIGINSTPQGLQIKVDGQTYTTPVNIVSVEGIWREIIPVSPQTTNNGVTY
                     DFVSWSNGSSKILSVQTPVNDISYTATFSTTLRTPENPQYSVSGMHYEYFEGSWNSLP
                     DFTLLLPKKEGNAANIDLTYKEVNDNFAFNFTGYIEIPTDGNYTFYCNSDDGSRLYIG
                     NSLVVDNDGLHSKYEASGTIGLKAGKHAMRVEYFEATGSEIFTVSYSGPGITKQIIPS
                     ASLFQDAVLYELVPIADSYIRSGSYANQTFGTTDELNLLVKKSKSTSNSLRVSYLKFD
                     ISGIDNAIISAKLKLNGYLNAPYPGGVTVGVWKTPNTYWNENLITFNNAPALPSNMIN
                     STIVNATTPVDYFWDVASYLDERRSGGYDKMTLAMANVFISSTSTVQFNSKEAPSDIP
                     KLFVLLNPNLLAFAKTENNYSLIESDILIYPVPANEECMVQLKINLADEYQYTITSLK
                     GEIIAKQNVSGNIFTIPTSSFDNGIYILTIQTTKGIIHKKFQVMH"
BASE COUNT         1063 a          604 c          602 g         1059 t
ORIGIN      
        1 tatctattca atgaataatg ctctcaaaac gagttattgt aagtaccgta tatacgtcat
       61 atctttaatt ttgttcaatt ttattgggat caattatgtt tatagtcaaa cctatccttc
      121 gggctttaca caggttgcag tagcatcagg tctttcaagt ccaacaaact tcgcttttgc
      181 acccgatggc cgaattttta tttgcgagaa agcaggtaaa ataaaaattc ttaaaaatgg
      241 aattttattg tcaactcctt ttctatcatt gaacgttaat aatgcagggg aaagaggttt
      301 gttaggaatt gcctttgatc caaattttac aactaacaag tatgtttatg tttattatac
      361 catcagtgct gctaccaata acagggtatc aagattcacg gcttcgggag atattgctat
      421 cagcaacagc gaagtaatcg ttcgaacaat ggacgcgtta tcaagcgcca ctaaccataa
      481 tgggggaaca ataaaatttg gtcctgacgg aaaactatat gttgcagtag gagaaaacac
      541 caattcaact gcttcacaat cacttacaac aaccatggga aagatgttga gaatgaattc
      601 ggatggaacc cctgccgttg gaaatccttt ttatagtagt accaatgcac aaaaaaaatt
      661 aatctggtgc tatggtttgc gcaacccata ttcgtttgat tttgacagta ataccggcaa
      721 gttgtttatt aatgatgttg gaagcaattc ttcagaagaa ataaatgatg gaacaaatgg
      781 aaatttaaat tttggttggc ctaatgttga gggaatcagt ggtaatgcag catatactga
      841 tcctgttttt gcatatgctc atggcgtaac aaatacaaca gggtgcgcca ttacaggagg
      901 ggcatttttt aattcttcta tttctaatta tccggccgca tatgcaaata aatattttta
      961 tattgactac tgcaatcagt ggataagtta tatcaacgtt tcaagccagt ctaattctac
     1021 tgtttttgcc acaggcatat cggacgaaat ggtttgtata gaccaaggga aagatggtaa
     1081 tttgtatttt ttatcgcgta tcgcaaacaa actttataag atttcgtata ataacattac
     1141 agtacccaca ataatttctc agccagtagg gctaagtgta tcattaggtc agcctgcctc
     1201 tttttctgta gttgccagtg gttcaacacc tttaacatat gtttggaaaa aaaatggagt
     1261 atctatcgcc aattctaact ctgctattta tactataaac aaggttacaa gcaatagtgc
     1321 gggaacctat agttgttata ttacaaattc atttggtagt gttacaagca caggggcagt
     1381 attggttgtt acagctttta atgccgctcc tgtggccaca atcatcacac ccaccaacaa
     1441 cacactttat tcgggaggaa tgaccgtgaa ttactcgggt actgctacag atgccgaaga
     1501 tggaaacatt gcttctgcaa attttctgtg ggaaattcgg tttttccatg atacccataa
     1561 tcacccgggg cctcttgcgg tagctggtgc caaatcaggc tctttcaata ttcctaatgt
     1621 tggtgaaaca tcagaaaacg ttttttaccg catatacctt tatgtaaaag atagtaaagg
     1681 attatatgat actgcatttg ttgatcttat tcctaaaact tcaattattg gaattaacag
     1741 tactcctcaa gggcttcaaa taaaagttga tggtcaaacg tatacaaccc ctgttaacat
     1801 tgtaagtgta gaaggtattt ggcgcgaaat tattcctgta agtcctcaaa ctacgaataa
     1861 tggggtcact tatgattttg tctcctggtc caatggctct tccaaaatat tgtctgtaca
     1921 aactcccgtt aatgatattt cttatactgc aacattttcc acaacattgc gcactccaga
     1981 aaatcctcaa tactctgtta gtggcatgca ttacgaatat tttgaaggta gctggaattc
     2041 actgcccgat ttcactttgt tattaccaaa aaaagaaggt aatgctgcaa atattgattt
     2101 gacatacaaa gaagtaaatg acaatttcgc ctttaatttt accggttaca ttgagatacc
     2161 tacagatgga aactatactt tttattgcaa cagtgacgat ggtagcagat tatatatagg
     2221 aaactcgttg gttgttgaca atgatgggct acattccaaa tacgaggcca gcggaactat
     2281 tgggcttaag gctggtaaac atgctatgag ggttgaatat tttgaagcca caggtagcga
     2341 aatcttcaca gtaagttata gtgggccggg aatcacaaaa cagataattc cttcagcctc
     2401 cttatttcaa gatgctgttt tgtatgaatt agttcccatt gctgatagtt acattcgatc
     2461 aggctcttac gcaaatcaaa cgtttggaac caccgatgaa ttaaacttat tagttaaaaa
     2521 gagtaaatca acatctaata gccttcgggt atcttattta aagttcgata tttctggaat
     2581 cgataatgct attataagtg ccaagctgaa gctaaatggc tatttgaacg ctccttatcc
     2641 gggtggtgta acagttggag tatggaaaac tcccaataca tactggaatg aaaatttgat
     2701 aacttttaac aatgctcctg ccttgccttc caatatgata aacagtacaa ttgtgaacgc
     2761 aactacaccg gtagattatt tttgggatgt ggccagttac cttgatgaaa gaagaagtgg
     2821 cggttatgat aagatgacct tagctatggc caatgttttt atatcttcta cgtcaactgt
     2881 ccaattcaat tccaaggaag ctccatctga tattccaaaa ttgtttgtcc tattaaatcc
     2941 aaacttattg gcttttgcaa aaaccgaaaa caattattct ctcatcgaat cagatatttt
     3001 aatttatcct gtgccggcca acgaagaatg catggttcag ctaaaaataa atttggcaga
     3061 tgaatatcaa tacacgatta caagtttgaa aggtgaaatt attgccaaac aaaacgtaag
     3121 cggcaatatt tttacaatac caacttcaag ctttgataat ggtatttaca ttttgactat
     3181 acaaaccaca aaaggaataa ttcacaagaa attccaggtt atgcactaac taatattctt
     3241 ttatataata tgttccgttt tttttatcgt tgtctaatat aaaagaaatc aatttacata
     3301 ttatattttt acaaccctcc tattcaaa
//
LOCUS       DAORQA010000197         3315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6892_length_3315_cov_45.096319, whole genome shotgun sequence.
ACCESSION   DAORQA010000197 DAORQA010000000
VERSION     DAORQA010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6892_length_3315_cov_45.096319"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PK736_08040"
     CDS             <1..201
                     /locus_tag="PK736_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC33379.1"
                     /translation="QLNMCDTNYGWTVEMQIKAAKHKMKYAEVPVNYRKRIGVSKVSG
                     TVKGVFGAGFKILYLVLKYAGK"
     gene            305..1792
                     /locus_tag="PK736_08045"
     CDS             305..1792
                     /locus_tag="PK736_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC33380.1"
                     /translation="MKIIFILILTLYATSLFLIFIYSLFQFHLSLLYLSNRKDSATIK
                     THPDSTYTPYVTVQLPIYNEYYVAERIIKSVAALDYPKDKLEIQVLDDSTDETKIVID
                     NVVEEIKNHFGTDINIIRRKHRVGYKAGALANGLEFAKGEFIAIFDADFLPSADFLKK
                     TISHFDEPNIAVVQAAWSHINLEYSLLTQMQAFGLDAHFTVEQTGRNCGNYFINFNGT
                     AGVWRKAAIIDSGNWQSDTLTEDLDLSYRAQLRGWKFKYVENIKAPAELPIEINALKS
                     QQYRWTKGAAETASKNIGKIIASKVGLSTKLHAISHLTNSGLFACVFIISILSVPVLY
                     IKWLTPQWNTFFKMATFSLISFAILSLFYFVAHVKPSPNKLKSALRFIIDYPIFLSLS
                     MGISLHNTIAVLGGYLGIKSEFIRTPKFNIANTKKFNSKNYKYASAPISVLNFFELLM
                     ALYFAFALMLAFRLRDFGLFPFHFMLFVGYSFVTFYTLKNAWQNR"
     gene            1773..3137
                     /locus_tag="PK736_08050"
     CDS             1773..3137
                     /locus_tag="PK736_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33381.1"
                     /translation="MRGKIVEYFTKANIVTAIIFGLLAFATANIPRTSFYTFIGAYAV
                     WFIFYLLICKNHTAQIEKEIGSSSSPIGFLKSLSAYQWIAILSRMVLIFSVPLLSDDV
                     YRFIWDGQIVAAGHNPFDYTPDWYMSHAHTIKNLNTDLYNQLNSKPFYTVYPPVCQAI
                     FGVSAYIGGASIKANIFCIKLFLLAFEIATIIILPKILKQFSINKNYAIVYLLNPLVI
                     IETIGNCHFEGIMVSLFLMAIYFFKKNNLTASAILFALSILTKLITVLGLPIILLLMP
                     WCQRFLYSATIAMVILISFSPIINNTMLQHFTDSVGLYFNYLEFNASIYTLLKSWGWK
                     IFNQDITTKYGFILPLISTAVIIYIYLQKHTRNFFTLLMAIFVVYYIFARTVHPWYLL
                     PIILFGTMSGFRFPIWWSAIVAATYITYSVTPYQENYWLVLFEYITVLVYAGYELKAR
                     RSRL"
BASE COUNT         1012 a          652 c          565 g         1086 t
ORIGIN      
        1 cagctaaata tgtgcgatac caattatggc tggacagtag aaatgcaaat aaaagccgcc
       61 aagcacaaaa tgaaatatgc cgaagtaccg gtaaattatc gcaagcgcat aggggtatca
      121 aaagtatcgg gtaccgtaaa aggggtattt ggcgctggat tcaaaatttt ataccttgta
      181 ttaaagtatg ccgggaaata aatttgttga caaacaaact tctgtcacaa ttactttgtc
      241 acccacccta ccatttcaga aaaaataaaa tattactatt gcgacacaaa attaaaaccg
      301 gggcttgaaa ataatattca tacttattct tacactgtat gcaacttcgt tgtttttaat
      361 ttttatttat agcctttttc aatttcatct ttcgctttta tatttatcta accgaaaaga
      421 ttctgccaca ataaaaaccc atcctgattc aacgtataca ccttatgtta ctgttcaatt
      481 gccaatctat aacgagtatt atgtagctga gcgaataatt aaatctgtag ctgcattaga
      541 ttacccaaaa gacaaactcg aaatacaagt gctggatgat tctaccgatg aaacgaaaat
      601 agtaattgat aatgttgtag aagaaataaa aaaccatttt ggcactgata taaatattat
      661 aaggcgcaaa catcgcgtag gatacaaagc tggcgcactt gctaatggct tagagtttgc
      721 caaaggagaa ttcattgcaa ttttcgatgc ggacttttta ccatcagcgg actttctaaa
      781 aaaaacaatt tcacatttcg atgaaccaaa catagcagtt gtgcaggcgg catggagtca
      841 catcaatctt gaatattcat tgttaacaca aatgcaggct tttggccttg atgctcattt
      901 tacagtggag caaactggcc gcaactgcgg aaattatttt ataaacttta atggcactgc
      961 cggtgtatgg cgcaaagctg caataattga tagtggcaac tggcaaagcg atacattgac
     1021 cgaagatttg gatttgagtt accgtgcaca actacgaggc tggaagttta aatatgtaga
     1081 aaatataaaa gcccctgcag aattacccat cgaaattaac gcgctcaaat cgcaacaata
     1141 tcggtggact aaaggtgctg cagaaactgc aagtaaaaat atcggaaaaa ttattgcttc
     1201 caaagttggc ctcagcacaa aactgcatgc catatcgcac ctcaccaaca gtggattatt
     1261 cgcctgtgtt tttattattt caattctcag cgtgcccgtt ttgtatatca aatggttgac
     1321 accacaatgg aatacttttt tcaagatggc aactttctcc ttaatcagct ttgccatact
     1381 ttcattgttt tattttgtgg cacatgtaaa accatcacca aacaaattaa aatcagcact
     1441 caggttcatt atcgactatc caatttttct gagtctgtct atgggaattt ctttacacaa
     1501 taccattgca gttttaggag gatatttggg aataaaatcc gaatttatac gcacaccaaa
     1561 attcaatatc gctaacacaa aaaagttcaa ctcgaaaaat tataaatatg ccagcgcacc
     1621 tatatcagtt ttaaactttt ttgaattgct aatggcattg tactttgctt ttgctttaat
     1681 gcttgccttt cgtctgcgcg attttggttt attccctttc cactttatgc tttttgttgg
     1741 gtatagtttt gtaacatttt atactttaaa aaatgcgtgg caaaatcgtt gagtatttta
     1801 ctaaagcaaa tattgtaact gccataattt ttggcctgct tgcttttgca actgcaaata
     1861 taccccgcac atctttttac actttcatag gtgcttatgc tgtttggttt attttctatt
     1921 tgcttatttg caaaaatcat actgctcaaa ttgaaaaaga aattggaagt tcttcgtcac
     1981 caattggatt tttaaaatca ctttctgcct atcaatggat tgcaattttg tcgcgcatgg
     2041 ttttgatttt ttccgtaccg ttattgagtg acgatgtgta ccgttttatt tgggatgggc
     2101 aaattgtagc agcgggccac aaccctttcg attacactcc cgactggtat atgagtcatg
     2161 cacacaccat taaaaacctt aacactgatt tatacaatca acttaatagc aagccatttt
     2221 acacggttta cccgccagtg tgtcaagcaa tttttggtgt ttcggcatat attggaggcg
     2281 catcaatcaa agcaaatatt ttttgcatca aactttttct gctagcattt gaaattgcta
     2341 cgataatcat tttgccaaaa attttaaaac agtttagtat caataaaaat tatgcaattg
     2401 tttatttgct taatcctttg gttataatcg aaacaatagg aaattgccac ttcgaaggca
     2461 tcatggtttc cctttttttg atggcaattt atttcttcaa gaaaaacaat ctgactgcat
     2521 ctgcaatctt atttgctctt tcgatactta caaaacttat aactgttctt ggcctcccca
     2581 taattttatt acttatgcca tggtgccaaa gattcttata ttctgcaact attgcaatgg
     2641 tcattttgat ttcattttcc ccaatcatta acaataccat gctgcaacat ttcacagata
     2701 gtgttggatt gtactttaat tatctagagt ttaatgcttc aatatatacc cttttaaaaa
     2761 gttggggttg gaaaattttc aatcaagaca ttacaacaaa gtatggattt atattaccat
     2821 tgatttcaac agcggtcata atttatattt acttgcaaaa acatacaaga aactttttca
     2881 ctttgctcat ggcaatattt gtggtgtatt acatttttgc ccgcaccgtg catccctggt
     2941 atttattgcc aattattctg ttcggcacca tgtcgggttt ccggtttcct atttggtgga
     3001 gtgcaattgt tgcagctaca tacataacat attctgtgac accatatcaa gaaaattact
     3061 ggctggtact gttcgaatac ataacagtac ttgtatacgc tggttatgaa ttgaaagcga
     3121 gaagaagtag attgtgattt acccaaatgc gccagccagt tagtgtaagc cccctcgcgt
     3181 cagctctctc gcgtcagccg ttgttggagt tttctccaac aaccacatgc gatagcccac
     3241 atgcgccagc cctcgtgcgt cagccgttgt tggagttttc tccaacaacc acatgcgata
     3301 gcccacatgc gccag
//
LOCUS       DAORQA010000198         3309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6908_length_3309_cov_5.725876, whole genome shotgun sequence.
ACCESSION   DAORQA010000198 DAORQA010000000
VERSION     DAORQA010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6908_length_3309_cov_5.725876"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PK736_08055"
     CDS             complement(<1..741)
                     /locus_tag="PK736_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33382.1"
                     /translation="MRKHLLMATACLLWSVFASAQLNVTFRSQLTFPGKSLANIWGYT
                     DSLSREYALVGTTAGMSIVDVTNATAPALLFNIVGANSQWREVRTYGKYAYVATEGGG
                     GLQIVNLSYLPDSAPSKFWTGNGPIAGLSRIHTVHVDGDNIFLNGCRNSGGSYLFNGA
                     SLICSLTDPWNPNFVGHTSTGVSSTVDYVHDGQALGNRLYAGHIYDGKFSIYDITNKA
                     APVLLNKQITTNAFNSFSAVYLKVSISCG"
     gene            complement(1227..1952)
                     /locus_tag="PK736_08060"
     CDS             complement(1227..1952)
                     /locus_tag="PK736_08060"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRC33383.1"
                     /translation="MKLFKNVFVVFFSIVVMYTMCSFIYENVTGSGKMAQENRTVPAF
                     SGIDVCCGIEVIVKSGSQSVSVNAEDNVLPLVETEVKDNVLHIGYKNKVSLKTHKKVT
                     VYVSAASIEKLYASSGSKLVCDEVIKGNKLIAKVSSGADMKIAIGVDDFNCEASSGSN
                     MQVSGKANSLWAEASSSGDIDAKNCPVSTATTEASSGSNITVNVSENLIAKASSGGDI
                     LYKGAVTKVNKKLSSGGSVKMLN"
     gene            complement(2076..>3309)
                     /locus_tag="PK736_08065"
     CDS             complement(2076..>3309)
                     /locus_tag="PK736_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRC33384.1"
                     /translation="VIIDWLADEFKNDEGLDLRKDPMALQRLKEAAEKAKIELSSSAQ
                     TEVNLPYIMPVDGMPKHLVKTLTRAKFEQLADKLIQSTLEPCKKALADAKLNPSQIDE
                     VILVGGSTRIPAIQDLVEKFFGRKPSKGVNPDEVVAVGAAIQGAVLTGEVKDVLLLDV
                     TPLSLGIETMGGVFTKLIESNTTIPTKKSEIFSTASDSQPSVEIHVLQGERSMAKDNR
                     TIGRFHLDGIPPAPRGTPKIEVVFDIDANGILHVSAKDQGTGKSQNIRIEASSGLSDE
                     DIKRMKNEAEANAESDRKLKEETEKLNLADTAIFSSEKQLKDYGDKIPADKKAAIESA
                     LAELKTAHSARNVDALDAASANLNNAWQAASQDINNAQQAEQANNANAQNNGADNGSA
                     NQDNKEGDVKDVDYEEVK"
BASE COUNT          933 a          685 c          588 g         1103 t
ORIGIN      
        1 accgcaagat attgaaactt tcaagtacac tgccgaaaac gaattaaatg catttgttgt
       61 aatttgttta ttgagcaata ccggagcggc tttgtttgta atgtcgtaaa tagaaaattt
      121 gccatcgtaa atatgccctg catataatct gttgcccaag gcttgtccat cgtgcacata
      181 atcaactgta gaacttacac cagtacttgt gtggccaaca aaatttggat tccatggatc
      241 ggtgagagaa caaattaatg atgcaccatt gaacagatat gaaccgccac tattgcggca
      301 gccatttaaa aaaatgttat cgccatccac atgcacagta tgtatgcgcg ataatccggc
      361 tattgggcca tttcccgtcc agaattttga aggtgctgaa tcgggcagat aagaaaggtt
      421 tacaatttgc aatccgcctc cgccttctgt agctacataa gcatatttac cataagtacg
      481 cacctcgcgc cattggctgt ttgcccccac aatattaaat aataaagcag gtgcagtggc
      541 attggtaacg tcaactatcg acatacctgc ggtagtgcca acaagtgcat attctcgact
      601 aagagaatcg gtatagcccc aaatattggc taaggacttt cctggaaaag ttaattgtga
      661 acgaaatgta acgttcaatt gtgctgaggc aaatacactc cataaaaggc atgctgttgc
      721 cattagtaaa tgtttacgca taaaaaaaat gttttaaagg atttaaagat tacaagcaaa
      781 taaagtcaaa aaaatctact tgccaaatat ttttatcctt taaccacaca ttattacatt
      841 ttgttgagct ttgtatactt tgatatgctt aaacgcttca aattagcttc cctgcaattt
      901 ctggttttct aaaattttgg taacgatagt tttttagttg aaagctataa ttaataaacc
      961 tgaataatgc aacacacatc aaattgatat gccatgtttg taatttctaa aaagaacaac
     1021 aatctgacta ttaaaaaaga aacaaaactt ttttggctgt atcaaaagtt agcgaggggg
     1081 gcgggtaaca atttcctcaa aaaaacagtg accaaataaa tatgagacat tattaatttc
     1141 aaaaaaaaga aagaataatc tgtgctttaa aatgcacaag aaattgaaca attaaattcc
     1201 tcttttgctt aaaatatttt ctacaatcaa ttcaacattt ttactgagcc acccgaagat
     1261 aattttttat tcacttttgt aactgcaccc ttataaagga tatcgccacc cgatgatgcc
     1321 tttgctatta aattttcgga tacatttact gtgatgttac tgccactcga agcctctgta
     1381 gttgctgttg aaaccgggca gttttttgca tcaatatcgc cactgctcga tgcttcggcc
     1441 cacaaagaat ttgctttacc acttacttgc atattgctgc ccgaacttgc ttcgcaatta
     1501 aaatcgtcaa ccccgattgc aattttcata tcggcaccac tcgatacttt tgcaataagt
     1561 ttgttgcctt ttattacctc atcgcataca agtttgcttc cactgcttgc ataaagtttt
     1621 tcgattgatg ctgcggatac atacaccgtt actttcttat gtgttttaag cgagacctta
     1681 tttttgtatc caatatgcaa cacattatcc tttacttcag tttctaccaa aggcaataca
     1741 ttatcctcgg catttactga taccgattgg gagccggatt tcacaatcac ttcgatacca
     1801 cagcaaacat caatgcctga aaatgccggc actgtgcggt tttcttgtgc catcttacct
     1861 gaaccggtaa cgttttcata tataaagcta cacatggtgt acattacaac aatggaaaaa
     1921 aatactacaa atacattttt gaataatttc ataagaagat gtttttagga gtttatatac
     1981 atctgacagt gagcaggtat gttttgttac aacagcacaa aaaaaatgcc tttgcaaacg
     2041 caaaggcatt ttttgttcca aatcgatttc gagaattatt ttacttcttc gtaatcaaca
     2101 tcttttacat cgccctcttt attatcctgg tttgcactgc cattatcggc accattgttt
     2161 tgtgcatttg cattgttggc ctgctctgct tgttgtgcgt tgtttatatc ttgtgatgcc
     2221 gcttgccatg cattatttaa gttggctgag gcggcatcaa gtgcatcaac attgcgtgca
     2281 ctgtgtgctg tttttaattc ggccaaagcg ctttcgattg cggctttttt atcggcagga
     2341 attttatcgc cataatcttt cagttgtttt tcgctgctga atattgctgt atcggcaagg
     2401 ttcaattttt cggtttcttc tttcaactta cggtcacttt cggcattggc ttcggcttcg
     2461 tttttcatgc gcttaatgtc ttcgtcactc aagcctgacg atgcctctat tcgtatattt
     2521 tgcgatttgc ctgtgccctg gtctttggcc gatacgtgca atattccatt tgcatcaata
     2581 tcgaatacca cttctatttt tggagtaccg cgcggtgccg gtggaattcc atctaagtgg
     2641 aaacggccaa tggtacggtt gtctttagcc atagaacgtt cgccttgcag cacatgtatt
     2701 tctactgatg gctgactatc ggacgcagtt gaaaatattt ccgatttctt tgtaggtatt
     2761 gttgtgttcg attctatgag ttttgtaaac acacctccca tagtttctat tcctaatgaa
     2821 agtggtgtta catcgagcaa caaaacatct tttacttcac ctgttaacac agcaccttgt
     2881 atggcagcac ctactgcaac tacttcatcg gggtttacac ctttcgatgg tttgcggccg
     2941 aaaaactttt ctaccaaatc ttgtatagct ggaattctgg ttgaacctcc aactaaaatt
     3001 acttcatcaa tttgtgaagg attaagtttt gcatcggcca aggctttttt gcatggctct
     3061 aatgtcgact gtattaattt atcagcaagt tgttcaaatt ttgcacgtgt taatgttttt
     3121 accaaatgtt ttggcatgcc atctacaggc ataatatatg gcaggtttac ctccgtttgt
     3181 gccgaactcg ataattctat ttttgctttt tcggcagctt ctttcaagcg ttgtaatgcc
     3241 ataggatctt tacgcaaatc gaggccttcg tcattcttaa attcatcggc aagccaatca
     3301 attattacc
//
LOCUS       DAORQA010000199         3292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6950_length_3292_cov_14.009577, whole genome shotgun sequence.
ACCESSION   DAORQA010000199 DAORQA010000000
VERSION     DAORQA010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6950_length_3292_cov_14.009577"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2141)
                     /locus_tag="PK736_08070"
     CDS             complement(<1..2141)
                     /locus_tag="PK736_08070"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HRC33385.1"
                     /translation="MYVVKRDGKQESVKFDKITARIQKLCYGLNADHVSSLSVAQKVI
                     EGLYDGVTTTELDNLAAETAATLTVRHPDYALLASRIAVSNLHKNTLKSFSETMRLLY
                     DYIDIKTGSKAPLIADDVWEVIEKNADLLDSTIIYDRDFGYDYFGFKTLEKSYLLKVH
                     GVVVERPQQMLMRVAVGIHKEDIDSVIETYNLMSERWFTHATPTLFNAGTPKPQLSSC
                     FLLTMQSDSIDGIYSTLHQCAKISQSAGGIGLSIHNVRATGSYIRGTNGTSNGIVPML
                     KVFNDTARYVDQGGGKRKGSFAIYLEPWHSDVFEFLDLKKNQGKDEIRARDLFFALWI
                     SDLFMKRVKSEGQWSLFCPNEAPGLSDCFGAEFEALYTRYEEEGRARKTIPARELWLK
                     ILESQMETGTPYILYKDAANSKSNQQNLGTIKSSNLCTEIMEYTSPDEIAVCNLASIA
                     LPRFVINGKFDHNKLFDITYVATKNLNKIIDINYYPVPEARNSNMRHRPIGLGVQGLA
                     DAFILLRMPFESDEARQLNRDIFETIYYAALTASKDLAIKNGPYQSYEGSPMSKGKLQ
                     FDMWNVTPSSRWEWDVLREEIKKHGVYNSLLVAPMPTASTSQILGNNECFEPYTSNIY
                     VRRVLSGEFVIVNKHLLSDLIKLNLWNNDMKNKLIAANGSVQNIKGIPDDMKELYKTV
                     WEIKQRTIIDMAADRGAFIDQSQSMNIFIQDV"
     gene            complement(2318..3241)
                     /locus_tag="PK736_08075"
     CDS             complement(2318..3241)
                     /locus_tag="PK736_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRC33386.1"
                     /translation="MYKIAEMSFWTAEEVDLYQDLRDWKNLSADEQHFIKHVLAFFAA
                     SDGIVNENLAVNFMSEVQIPEARCFYGFQIMIENIHSEMYSLLIDTYIKDTAEKAYLF
                     NALETVPCVKKKADWALKWIETGSFAERLVAFACVEGIFFSGSFCSIFWLRKRGLMPG
                     LTFSNELISRDEGQHRDFACLLYGMLHNKLSQERIYEMIKDAVACEHEFVTDALPVSL
                     IGMNGKLMCQYIEFVADHLLAQLGYPKLYNSENPFDFMDMISLQGKTNFFEKRVSEYV
                     KASVKNKMMESINNAKNANNIVDNFNFDEDF"
BASE COUNT          951 a          695 c          593 g         1053 t
ORIGIN      
        1 acatcctgaa taaaaatatt catgctttgg ctttggtcta taaatgcgcc acggtcggca
       61 gccatatcga taatggtgcg ctgttttatt tcccacacag ttttatacaa ctctttcata
      121 tcatcgggaa tgccttttat attttgtacc gaaccatttg cggctatcaa cttgttcttc
      181 atatcgttgt tccacaagtt cagttttata aggtcgctta ataagtgttt attcacaatt
      241 acaaactcac cactcaacac gcgtcttaca tatatattac ttgtgtaagg ctcaaagcat
      301 tcgttgttgc caagtatttg tgaggtactt gctgtaggca taggtgctac aagtaatgaa
      361 ttatacacgc catgtttttt tatttcctca cgcagtacat cccactccca gcgcgaagat
      421 ggagttacat tccacatatc gaactgcagc tttcctttgc tcatagggct gccttcataa
      481 ctttgataag ggccattttt tattgccaaa tctttacttg ctgtaagtgc agcgtaataa
      541 atagtttcga aaatatcgcg attaagctga cgtgcctcat cgctttcgaa tggcatacgc
      601 aataaaataa atgcatcagc aagaccttgc acacccaagc cgatagggcg atgacgcata
      661 ttgctattac gcgcttcggg tacaggataa taattgatgt caattatctt gttcagattt
      721 tttgtggcta cataagtaat atcgaacaat ttattgtgat cgaatttgcc attgataaca
      781 aaacgtggta acgcaataga agctaaatta caaacggcaa tttcatcggg cgaggtgtac
      841 tccattattt cggtacagag gttactactt tttatagtac ctaaattttg ctgattgctt
      901 tttgagtttg cagcatcttt atataaaata tatggcgtgc cggtttccat ttggctttcg
      961 agaattttga gccacaactc gcgtgcggga attgttttac gtgcacggcc ttcttcttca
     1021 tatcgggtat acaatgcttc gaattctgca ccaaagcaat cgctcaatcc tggtgcttcg
     1081 ttagggcaaa acaaactcca ttgcccttcg ctctttacac gtttcataaa taaatccgaa
     1141 atccataaag cgaaaaataa atcgcgcgca cgtatttcat ctttaccctg attctttttt
     1201 aagtctaaaa attcgaaaac atcgctatgc cacggttcta aatatatggc gaacgatcct
     1261 ttgcgttttc ctccgccttg atctacataa cgtgctgtat cattaaaaac tttcaacatt
     1321 ggaactattc cattcgatgt gccattggta ccacgaatgt aactgcctgt tgcacgtacg
     1381 ttgtgtatgc taagaccaat gccgccagcg ctttgcgata tttttgcaca ctgatgtaag
     1441 gtgctgtata taccatcaat actgtcgctt tgcatggtga gtaaaaagca tgatgacagt
     1501 tgtggcttag gtgtaccggc attgaacaat gttggagtag catgtgtaaa ccaacgttcg
     1561 ctcataaggt tatatgtttc tataacgcta tcaatatcct ctttgtgaat acctactgcc
     1621 acgcgcatga gcatttgctg tgggcgttcc acaacaacac cgtgtacttt taataagtat
     1681 gatttttcta aagttttgaa accaaaataa tcgtaaccaa aatcgcggtc ataaataatt
     1741 gtactatcaa gaaggtcggc atttttttct attacctccc acacatcatc agcaatcaat
     1801 ggagctttgc ttccggtttt aatatcgatg taatcgtata gcaaacgcat cgtttcactg
     1861 aaagatttca atgtgttttt atgcaaatta cttaccgcaa ttcgcgatgc cagtaaagca
     1921 tagtcgggat gacgtactgt aagtgttgcg gcagtttcgg cagcaagatt gtccagctct
     1981 gtagtggtaa ccccatcgta caacccttca ataacttttt gtgctacact cagcgagctc
     2041 acgtgatctg cattgaggcc ataacacagt ttttgaatac gcgcagttat cttatcgaac
     2101 tttacgcttt cttgtttgcc atctcttttt actacataca tgatttaatc aggggtttaa
     2161 ggagtttgat ttagttatcg gacaaaggca cacagggtgt ataaactgcg tgtcttttta
     2221 ttattattta agaataacaa agcggtgcaa ttttcaaatt agaatataca agcaattgac
     2281 tgatgctttg ttgcgtaatg ataacgctta gtgagattta aaaatcttca tcgaaattaa
     2341 aattatccac aatgttatta gcattctttg cattgttgat agactccatc attttgttct
     2401 ttaccgaagc ttttacgtac tcgcttacac gcttttcgaa aaagttggtt ttgccttgca
     2461 acgatatcat atccataaaa tcgaaagggt tttcgctatt atacaattta ggatagccca
     2521 attgtgctaa taagtgatcg gccacaaact cgatgtattg acacatcaat tttccattca
     2581 ttccaatgag cgataccggc aaagcatctg taacaaactc atgctcgcat gctacggcat
     2641 cttttatcat ttcgtaaata cgctcttgcg aaagtttgtt gtgcaacatg ccataaagca
     2701 gacatgcaaa gtcgcggtgc tgtccttcat cgcgcgatat aagttcgttg ctaaatgtta
     2761 agccaggcat aaggccacgc ttacgcaacc aaaatataga acaaaacgaa ccgctaaaaa
     2821 atattccttc tacacatgca aatgcaacaa ggcgttcggc aaaagaaccg gtttcaatcc
     2881 acttcaatgc ccagtcggct tttttcttta cacaaggcac tgtttcaagg gcattaaata
     2941 gataagcctt ttcggcagtg tcctttatat atgtatcaat taacaacgaa tacatttcgc
     3001 tgtgaatatt ttctatcatt atttgaaaac cataaaaaca acgtgcttca ggaatttgca
     3061 cttcgctcat aaaatttaca gcaaggtttt cgttaactat accatcgctt gcagcaaaga
     3121 atgctaaaac gtgttttata aaatgctgct catcagcact aaggtttttc caatcacgta
     3181 agtcctgata tagatccact tcttcggcag tccaaaaact catttccgca attttataca
     3241 tttatgacat ggaattcgac acgtacacgt gagtggacac aaggtagcaa ca
//
LOCUS       DAORQA010000200         3289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6952_length_3289_cov_23.477118, whole genome shotgun sequence.
ACCESSION   DAORQA010000200 DAORQA010000000
VERSION     DAORQA010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6952_length_3289_cov_23.477118"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(465..872)
                     /locus_tag="PK736_08080"
     CDS             complement(465..872)
                     /locus_tag="PK736_08080"
                     /inference="COORDINATES: protein motif:HMM:NF025613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4348 domain-containing protein"
                     /protein_id="HRC33387.1"
                     /translation="MKSILFICIIILTGCGNKSLDKTTTDVLTEENLAEFFDQFKSDS
                     IFQIERVKFPWKIPSDGKELVINKAEWQHADFLYHDNYATREIDAYTQEIVTYGDTVK
                     IEQRGVDNGIHIDFVFAKIDNKWFLCFEKDLSN"
     gene            complement(1458..2732)
                     /locus_tag="PK736_08085"
     CDS             complement(1458..2732)
                     /locus_tag="PK736_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRC33388.1"
                     /translation="MEILIIIGLVLLNGIFSMSEMSLVSSRKFKLENAKKKGSKGAKT
                     ALELSDNPTKFLSTVQIGITLIGILLGVYSGENLTNNVSYYLVQFELIKDYAHNIATG
                     IIVVFVTYLSIVLGELLPKRIAMAFPEPIAIVLAQPMKILSVITSPFVWLLTTTNDIL
                     LKIMGIKKTTDSKVSEEEIKSIIKESAEGGEIQDIEHNIMERVFELGDRKVNTLFTHR
                     SDIVYFTIQDSWEEIKFKINQEKHSAYPVSKSNNLDDIIGIVLLKDLFTPTSDKNFNI
                     KDYLKVPIYFNEGTYAYKVLETFKKEKMHYGIVVDEFGVTQGIVTMDDVVDALVGDVT
                     EIDQDEYQLTQRDENTWLVDGQYSIIDFVKFFNIELKENYQSKYTTVAGLIIYNNNTL
                     PEIGQKLTIDNYELEIIDKDGQRIDKILVTKK"
BASE COUNT         1024 a          602 c          548 g         1115 t
ORIGIN      
        1 ctgccgcaac caaatggaaa tatcaagttt agattgcaac atcgatactg aaaatattgt
       61 acgctttatc tatgcctttg tttgttaact ccatttagtg aaattgaaat ttgaaagagg
      121 aattgttgtc gtggtatttt aaactacaag agacataaag cgtatattgt taccgttacg
      181 gaattgtttc ggctggcaaa attttccaac gtttgtgttt gcgcagtggt ggactttcga
      241 agccgcgtcc tgtccccgac acaaaaacga tgaacgaaga taacactttg aacttacacg
      301 tcaccccacc attgcgcaaa cattttgttg ggtgatgggc ggttcagtcg gtagttaatt
      361 gttccagagt ctttgtcgac cgttgaagtc ttgttagtca aacgacttgt tatcgccagg
      421 taactttgag gtcagacgga aacgtccagc ggaaataaat ttggtcaatt agaaaggtct
      481 ttctcaaaac acaaaaacca tttattgtca attttagcga acacaaaatc aatatgaatt
      541 ccgttgtcta cgccacgttg ttcaattttt actgtgtcac cataagttac aatttcttga
      601 gtgtatgcat caatttctct tgttgcgtag ttgtcatggt ataagaaatc agcatgttgc
      661 cattcagcct tgttaataac taactctttt ccgtccgatg gaattttcca tggaaatttt
      721 acacgctcaa tctgaaaaat cgaatcactt ttaaattggt caaaaaattc agcgagattc
      781 tcctcggtta aaacatctgt tgttgttttg tcaagagatt tatttccaca acccgtcaat
      841 attattatac aaatgaatag aatcgatttc atctttgttg tcaatggtag agctatttta
      901 gagtcacata aaaaacttgt caattataca gtcacttttt cgtcagcaac gccttgcacc
      961 tagcatcatc ccgtgaaaaa ccaattcatc aataactgca cgttaacaac atgtggtaaa
     1021 aataaaaatt acaacgacac cgttgacata ccttagcaaa aaaaataaat atgcatcaca
     1081 tagctgaggg ctgccggcat caaatggaaa tatcaagttt agattgcaac atcgatactg
     1141 aaaatgttgt gcgctttatt gatgcttttg tttgaaagct caatttagta aaattgaaat
     1201 ttgaacgagt aatagttgcc cctgtatttt aaattgcaaa aggagccaaa aatcgtatat
     1261 ggttaccgtt acggaattgt ttcggccgcc aaaattttcc aaaatgggtt ttttcacatc
     1321 ctgacgtttt gcagctaaaa ccggtgcggg attagaagaa ccgtctgctt agtaaatgta
     1381 ctacagaata ttagaattcc aaatgattga aaagctacac tgcccgcatt ggttattaac
     1441 ggtggttagt ggcagtgcta ttttttagta actaatattt tgtcaattct ttgcccgtct
     1501 ttgtcaatta tttcaagttc ataattgtca atagttaatt tttgaccaat ttcaggcaat
     1561 gtattgttgt tgtatattat caatcctgca actgtggtgt attttgactg atagttttct
     1621 tttaattcta tgttaaaaaa tttaacaaag tcaataattg aatattgtcc atctactaac
     1681 catgtatttt cgtctctttg ggtcaattgg tattcgtctt ggtcgatttc ggttacatca
     1741 cctactaatg cgtccactac atcatccata gtaacaattc cttgcgttac accaaattca
     1801 tcaactacta ttccatagtg cattttttct tttttaaatg tttcaagcac tttgtaagca
     1861 taagtacctt cgttaaaata gattggcact tttaaataat ctttaatgtt gaaatttttg
     1921 tcagaggttg gtgtaaataa gtcttttaaa agtacaatac caattatatc atctaagtta
     1981 ttgcttttag atactggata tgctgaatgt ttttcttgat tgattttgaa tttaatttct
     2041 tcccaagagt cttgaattgt aaaataaact atatcacttc tatgtgtaaa tagtgtgttt
     2101 acttttctgt cacccaattc aaaaacacgt tccataatgt tgtgttcaat gtcttgaatt
     2161 tcgccacctt cagcactttc cttaataatt gattttattt cttcttccga aactttgctg
     2221 tctgttgttt ttttaattcc cattattttc agcaaaatat cattggtggt tgtcaaaagc
     2281 caaacaaagg gtgaagtaat tacagataga attttcattg gttgtgccaa gacaatagca
     2341 ataggttcag gaaaagccat tgcaattctt ttgggtaata attcgcctaa gacaatagat
     2401 aaataggtta caaaaactac aattatccct gtagctatat tatgagcata gtccttaatt
     2461 aattcaaatt gtactaaata ataagaaaca ttgtttgtta aattttcgcc actgtacaca
     2521 ccaagcaaaa tcccaattaa ggtaatccca atttgcacgg tgcttaaaaa tttagttgga
     2581 ttatctgaaa gttctaaggc tgttttcgcg cctttactgc ctttcttttt tgcattttca
     2641 agtttaaatt ttcttgatga aactaatgac atttctgaca ttgagaatat tccgttaagt
     2701 aaaactaaac ctattattat taaaatttcc attttctgtt tgtcatatta attaatcttt
     2761 tttaagggtt ctacggcatt gccactaacg tcagcctgtg aaaaaaccat ttcctccaca
     2821 actgcacgtt agcaacatga ggtaaaaata aaaattacaa cgacactgct gacatacctt
     2881 agcaaaaaaa aatatacatc acatggctga gggaagccgg cataaataga aatatcaagt
     2941 ttagattgca acatcgatac agaaatgttg tatgctttat tgatgctttt gtttgcaagc
     3001 tcaatttagt aaaattgaaa tttggagtgg aattgttggc cttatatatt agagagctaa
     3061 aaacgccaca aatcgtatac agccatagtt aaacaatcgc gaaaaacaaa attgctttac
     3121 taatacacct aagcaattta gtgcgccctc ctcagcaaag ccactcaatt atgacgacac
     3181 cccataccgg tgtcgctgaa caccccgtta gagtacacgg ggcaggccta tagtcaaaag
     3241 cccctcgtat tcgactcgcc cgggggcttt cgtctatacg gctaaatgt
//
LOCUS       DAORQA010000201         3287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6964_length_3287_cov_6.143564, whole genome shotgun sequence.
ACCESSION   DAORQA010000201 DAORQA010000000
VERSION     DAORQA010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6964_length_3287_cov_6.143564"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3287
                     /locus_tag="PK736_08090"
     CDS             <1..>3287
                     /locus_tag="PK736_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33389.1"
                     /translation="VYNLSSANCSDIFSNPSLEVACNFAGTPGVTGPNGLPGAQNEPP
                     IPVLIGETYVINVSQFTVSPNGYTISFGLSTASILDITPPAIDSVNTNFVCGQDSFQV
                     TFTENIQCSSVQISDFVLTDPQGNPMTITSVTSPNCGSVCGYARTFIFKFTPGANFLG
                     AYTLDIISNIDDVCSNSCLIPTSIIFNIGSYVYNNVVTNVTCPGGADGAINVSVTSPP
                     GPYTYLWSNGNTTANPTGLSAGTYTVTVSKGGGCPDIKTFIITAPTAWAYVTASTTSA
                     CGATTGSASVTVNAGGTGPYTYLWSSAATTSNATGLGAGTYTVTVKDANNCTVTATVA
                     VNQTAGVVASIPANVNVNCFGASTGTATGSGANGSGGYTYLWSNAQTNATAVGLAAGT
                     YTVTVTDNVGGCTSTATVVITQPATAVTAVTNTQTTTCGLNNGLAYVTGGGGTGPYTY
                     LWSNANSNDTISPVASGTYTITVTDSKGCTKTATAVVGASSSVTASIPNSVNVSCNGG
                     TNGTATAGCANCTGAMTYLWSNAQTIQTATGLASGTYTVTVTDANNCTSTVSVNITQP
                     TVLSIINALNDTICLSQFSSITPAGSGGTGPYGYSWSNGNTNALLSVSPAVTTSYTVT
                     VTDNKGCTKTQSVTVYVSPALTVTASNDTTICSGSAVNVSCLAGGGNTTYTYNWNGGL
                     NITSGFIVNPTKDTTLIIKLTDNCGTPAVYDTVHIYVITPMVLNFPTVVNVSCNGGSN
                     GSATVAIVGGVPVFVYAWSNGQTNATSTGLASGTYTVTITDGNTCSMSSSVVITQPTA
                     LAITGMPNATICLSQNATITPVVNGGTPTYTYIWSSAQTTSTITVSPTTATTYTVTVT
                     DSKGCTKTQSVTITVNPALTVTAPKDTIICSGAGVTVTASPGGGNGAFIYSWNGGLNS
                     TNSFTINPTADTTLIIKLTDNCGSPAVYDTMHITVVTPMVLTFPIVVNVSCNGGNNGS
                     AQVSVNGGIPAYAYLWSNGQTTNTATGLAAGNYTVTLTDGNNCTMSATVNITQPTALT
                     ITGMANVTICLSQNTTITPVYGGGTPAYSYSWSSGSTAASITVTPNSTTSYTVTVTDA
                     KGCTLTQ"
BASE COUNT          927 a          745 c          677 g          938 t
ORIGIN      
        1 gtttataatt tatcaagtgc caattgctcc gatatttttt ctaaccccag ccttgaagta
       61 gcttgcaatt ttgctggaac tcctggtgta acaggaccta atggattacc aggtgcacaa
      121 aatgaacctc ccattcctgt attgataggc gaaacctatg ttatcaatgt aagtcagttt
      181 actgtgtcgc ctaatggtta tactattagc ttcgggcttt caacggcaag tattttggat
      241 atcacgcctc ccgcaatcga cagtgtaaat acaaactttg tttgtggtca ggactccttt
      301 caagtaacat ttactgaaaa catccaatgt tcttcggttc agatttccga tttcgttttg
      361 actgatccac aaggaaaccc tatgacaatt acaagtgtta catctcctaa ttgcggttcg
      421 gtttgtggct atgcccgtac attcattttt aaatttactc cgggagcaaa tttccttgga
      481 gcatacacac tcgatattat tagcaacatt gatgatgtgt gttccaattc gtgcttgatt
      541 cctactagta tcatttttaa tattggctca tatgtttata ataatgtcgt cacgaatgtt
      601 acttgcccgg gtggtgccga tggtgccata aatgtttcgg taacttctcc gccaggtcca
      661 tacacttact tatggtcgaa tggcaatacc accgcaaacc caacaggatt atcagccggc
      721 acgtataccg ttacagtttc gaaaggtgga ggttgccccg atattaaaac atttatcatt
      781 accgccccta ctgcctgggc ttatgttact gcctccacca cttctgcatg tggagcaacc
      841 acaggttcgg caagcgttac agttaatgct gggggaactg gcccttatac ttatttatgg
      901 tcatcggctg ctactacttc aaatgctacc ggccttggcg ctggcacgta tacagttaca
      961 gttaaggatg ccaacaactg tacagtcact gcaactgtgg cggtgaatca aacagcaggt
     1021 gtggtagctt caatacctgc taatgtaaat gtcaattgct ttggagcctc cacaggtaca
     1081 gcaaccggca gtggagccaa tggttccgga ggatacactt atctatggtc taatgcacaa
     1141 accaatgcaa cggcagtagg tttggctgct ggtacctata cagtaactgt aaccgacaat
     1201 gtgggtggat gcacttcaac tgccactgta gttattacgc aaccggctac agcggtaact
     1261 gctgtaacaa atacacaaac caccacctgt ggattgaata atggcctggc ctatgtcaca
     1321 ggaggcggtg gaacaggccc ttatacatat ttatggtcaa atgcaaacag taacgacact
     1381 atttctcccg tagcttctgg tacgtatact attactgtaa cggatagtaa aggctgtact
     1441 aaaactgcaa cggcagttgt tggtgcatct tcttcggtaa cagcaagtat acccaatagc
     1501 gtaaacgttt cgtgcaatgg tggtacaaat ggtacagcca cagcaggttg cgccaattgt
     1561 accggtgcta tgacttattt gtggagcaat gctcaaacca ttcaaactgc aacagggctt
     1621 gcctcgggca catataccgt cactgtaacc gatgccaaca attgcacttc aaccgtttcg
     1681 gttaacatca ctcaaccaac agttctgtca attattaatg cattaaatga caccatttgc
     1741 ctaagccagt tttcgagcat tacacctgcc ggaagtggag gaacagggcc atacggctat
     1801 tcgtggagta atggaaatac gaatgcatta ttgagcgtaa gcccggcagt tactacatcg
     1861 tatacagtta ctgttaccga taataaaggt tgcacaaaaa cacaatcggt tactgtctat
     1921 gtttcacctg cgctcactgt taccgcatca aacgacacta ctatttgttc gggcagtgct
     1981 gtaaatgtga gttgccttgc aggtggaggt aatacaactt acacctacaa ctggaatgga
     2041 ggactcaata tcacatccgg ttttattgtt aacccaacaa aagatacaac cttaattata
     2101 aagcttacag ataactgtgg cacgcctgcg gtgtacgata cagttcacat ttatgttatt
     2161 actcctatgg ttttaaattt cccaactgtg gtaaatgtat catgcaatgg tggaagcaat
     2221 ggttcagcaa ccgtggctat tgttggtgga gttccggttt ttgtttatgc atggagcaat
     2281 ggtcaaacca atgctacatc aactggctta gcaagtggca catacactgt tactataaca
     2341 gacgggaaca cctgttcaat gtcttcatct gtcgtaataa cacaaccaac agcattggct
     2401 ataactggta tgcctaatgc cacaatttgc ttgtcacaaa atgcaactat tacccctgta
     2461 gtgaatggtg gtactcctac ttatacttac atatggagtt ctgcgcaaac aacaagtacc
     2521 ataacagtaa gccccacaac tgccacaacg tatactgtaa cagttaccga ttcgaagggt
     2581 tgtaccaaaa cccaatcggt tactataacc gttaatcctg cattaactgt taccgctcct
     2641 aaggatacca ttatttgcag tggcgcaggt gttacagtta cagcttctcc gggcggagga
     2701 aatggcgcat ttatttatag ctggaatggg ggattaaatt caacaaactc gtttacaatt
     2761 aatccaacag ctgatacaac tttgattata aagttaaccg ataactgtgg tagtcctgcg
     2821 gtttacgata ctatgcacat tacagttgtt actcccatgg tattaacttt tccgattgta
     2881 gttaatgttt cttgcaatgg cggaaataat ggttcggcac aagtaagcgt aaatggtggt
     2941 ataccggcat acgcctacct ttggagcaat ggacaaacca caaatactgc tacaggactt
     3001 gcggcaggaa attatacggt aaccttaacc gatggcaata attgtactat gagtgctaca
     3061 gttaatatca ctcaacctac tgcacttacc attacaggta tggccaacgt gaccatttgc
     3121 ttatcacaaa acactactat cactccggtg tatggtggtg gtactcctgc atattcatat
     3181 tcatggagct cgggctccac tgctgcaagt attacagtta ctcccaatag cactacatcg
     3241 tatacggtaa ctgttaccga tgctaaagga tgtacgctaa cccaaag
//
LOCUS       DAORQA010000202         3286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_6966_length_3286_cov_15.223460, whole genome shotgun sequence.
ACCESSION   DAORQA010000202 DAORQA010000000
VERSION     DAORQA010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6966_length_3286_cov_15.223460"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PK736_08095"
     CDS             complement(<1..754)
                     /locus_tag="PK736_08095"
                     /inference="COORDINATES: protein motif:HMM:NF025720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HRC33390.1"
                     /translation="MNRLQNFFWFCSGANIALLKKTPTEQTKYAGIGATVFFTGLFAT
                     VSGAYALYFIFNDANSTVRITASLLFGLIWGAMIFNLDRYIVSSMKKNGSTLSEFKLA
                     APRFLLAAIIAFVISKPLELQIFHTSIEYELELMKQEEIKKQEVALTSRYQNDMNLLD
                     SEIKNLQKDIDTKTNYRNKADEEARKEADGSGGTMKRGAATIYNLKKADANKAQQELE
                     ELTTTNKPLIDAKQKLLFDKKRTVDSLKASVKR"
     gene            1171..3084
                     /locus_tag="PK736_08100"
     CDS             1171..3084
                     /locus_tag="PK736_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRC33391.1"
                     /translation="MERLQKLRELLKIERDEDYEQYKEHFSRNNINHRKQHGVTWYPI
                     KINNTEIGFGDYISIEIERTNGLNESHKFSGGKVASLFSNVHTQATPINGTIKMLGQN
                     KMRLSLNIDELPDWCSDGKLGINLLFDETGYKEMDIALEKVIKADNNRLAHLRDVMYG
                     ITVPQFEKEIDSLQITGLNESQNRAVKKIIAAKDIAIIHGPPGTGKTTTLVKAICEVL
                     KTEKQVLVCSPSNTAVDLITEKLHREKINVLRLGNPARISDEVMMNTLDAKISSHASY
                     KEIKNFKKLAEEYFRMAGKYKRVFGKEDREQRTLLYKEARKIMQEARSLEEYITNDQF
                     EKAQVITCTPVVSANKTMRDKQFTTLFIDEAAQALEPVCWIPISRCDRVIFAGDHFQL
                     PPTVKSKKAEAQGLSETLFEHCMNIENVSVMLNTQYRMHQHIMDYSNEKFYGGNLIAH
                     QSVQEARLINDSDEPILSTALTFIDTAGCGYTEQMNSESMSICNHDEAVLLINHLKML
                     LTQYHEKSKRKITVGIISPYKEQVQYINSLIAADAELQNSHTKIVVKTVDGFQGQERT
                     VIYISLVRSNDKKEIGFLNDTRRMNVAMTRAREKLVVIGDSATLANHPFYKNFIDYAE
                     AIGAYVSAWEFIA"
BASE COUNT         1081 a          624 c          645 g          936 t
ORIGIN      
        1 cgcgcttcac acttgccttc aaactatcta ctgttcgttt tttatcgaaa agcaatttct
       61 gctttgcatc aattagcggt ttgttggttg ttgtaagttc ttcaagttcc tgctgtgctt
      121 tattggcatc ggcttttttc aaattataaa tagttgccgc accacgtttc attgtgcctc
      181 cgcttccatc ggcttctttg cgggcttctt cgtctgcttt gtttctgtaa ttcgttttag
      241 tatcaatatc tttttgcaaa ttttttattt ctgaatccag caagttcata tcattttgat
      301 aacggcttgt gagtgccact tcctgctttt ttatttcttc ctgcttcatc aattccaact
      361 cgtattcgat tgaagtatga aaaatctgca actccaatgg tttcgaaatt acaaatgcaa
      421 tgatggcagc aagcagaaat cgtggcgctg ctaatttaaa ttcgcttaat gtgctgccat
      481 tttttttcat gctcgatact atgtatctgt cgagattaaa aatcatagcg ccccatatta
      541 aaccaaataa taaagatgct gtaatacgaa cggtggaatt cgcatcgtta aaaataaaat
      601 acaatgcata tgcccccgaa actgttgcaa acaatccggt aaaaaaaacc gttgctccta
      661 taccggcata tttagtttgt tcggtgggtg ttttttttag taaagcaata tttgcccccg
      721 aacaaaacca gaaaaaattt tgcagtcgat tcattgatat attttggttt aaagtacctt
      781 tcttttcggt gatgaatagt taatttatac tcatccaaag tattgacgca gaaagtttca
      841 atttgtttcg ctttttcata aatttatttg aaaggaattt acaaagcatt gattgctccg
      901 ttccgctcat aatgaccatt atgaccattg actagaatgc cgacactttg gtcggcatcc
      961 cgaccgaagt gtcggggttt aaaatgacca atgacaatcc tcaatgactg gaagagattg
     1021 cttcgcagtt cgggaatact cactgtgaca attcgcaatg acgataatgc ccaatgacaa
     1081 gaattcataa taactaaata accagtaaca aaataactac accaaaattt tccttaattt
     1141 acatttcgaa acgaaacaca aattcaacaa ttggagcgat tacaaaaact acgcgagcta
     1201 ttaaaaatag aacgtgacga agattacgaa cagtataaag aacatttttc gcgtaacaac
     1261 attaaccacc gcaaacaaca tggtgtaaca tggtatccta taaaaataaa taataccgaa
     1321 ataggttttg gcgattatat ttcaattgaa atagagcgca caaatggtct taatgaaagt
     1381 cataaatttt cgggaggcaa agtggcatca cttttttcga atgtgcatac acaagccaca
     1441 cccatcaatg gcacaataaa aatgcttggg caaaataaaa tgcggctgtc gttgaacatc
     1501 gatgaacttc ccgattggtg cagcgatggt aagcttggca tcaatttact gtttgacgaa
     1561 acgggctata aggaaatgga tattgccctc gaaaaagtaa taaaggccga caacaatcgc
     1621 ctggcacatt tgcgcgatgt gatgtatggt ataacagtac cacaatttga aaaagaaatt
     1681 gactctttac aaataacagg tttgaacgaa tcgcaaaacc gtgcggtaaa aaaaataata
     1741 gcagcaaaag atattgctat catacatggc ccacccggca ctggcaaaac cacaacactt
     1801 gtaaaagcaa tatgcgaagt attgaaaacc gaaaagcaag ttttggtatg cagcccaagt
     1861 aacaccgctg tagatttaat aaccgaaaaa ttgcatcgcg aaaaaataaa tgttttgcgg
     1921 ttgggcaacc ctgctcgtat ttcggacgag gtgatgatga acactttgga tgcaaaaata
     1981 tcaagtcatg cttcctataa agagataaaa aattttaaaa aactggccga agaatatttt
     2041 cgcatggcag gaaaatacaa acgtgtattt ggaaaagaag atcgcgagca acgtacccta
     2101 ctttataaag aagcacgcaa aataatgcaa gaagcacgct cgctcgaaga atatattacc
     2161 aacgatcaat ttgaaaaagc acaggtgata acatgcaccc ccgtagtgag tgcaaacaaa
     2221 actatgcgcg ataagcaatt tacaactttg tttatagatg aggccgcgca ggctttggag
     2281 cccgtttgct ggatacctat aagccgatgc gacagagtga tttttgccgg agatcatttt
     2341 caattgccac caacagtgaa atcgaaaaag gccgaagcac aggggctgag cgaaacatta
     2401 tttgagcatt gtatgaatat agaaaatgta tcggtgatgt tgaacacgca atacagaatg
     2461 caccaacata tcatggatta ttctaacgaa aagttttatg gcggaaattt aattgcacac
     2521 caatcggtac aggaggctcg tttgataaat gatagtgatg aacctatttt aagcaccgcc
     2581 ctcactttta ttgatactgc agggtgtgga tatacggagc aaatgaattc cgaaagcatg
     2641 agcatttgca atcatgatga agcagtgcta ttgataaatc atttgaaaat gttgctcact
     2701 caatatcacg aaaaaagtaa aaggaaaatt acggtgggaa ttatatcacc ttacaaagag
     2761 caagtgcaat acattaattc gctgattgca gccgatgcgg aattacaaaa ttcacacaca
     2821 aaaattgttg taaaaacagt tgatggattt cagggacagg aacgaaccgt aatttatatt
     2881 agccttgtac gcagtaacga taaaaaagaa attggttttt taaatgatac cagaagaatg
     2941 aatgttgcta tgacccgtgc ccgcgaaaaa ttggtggtga taggtgatag tgccacactt
     3001 gccaatcatc cgttttacaa aaattttatt gattatgccg aagctatagg tgcttatgtt
     3061 tcggcttggg agtttattgc atagtcgatt ttttttgcca cgcctgccgc gaccgcagtg
     3121 tcggggaatg cacgaatgaa tttcactttc ttcccgtcat tgcgaaggag gtacgactga
     3181 agcaatctca gcggtcacaa gatgttgtgg cgcagagatt gcttcgttgc acttacaatg
     3241 acatcctcgt aaaagacaga ttatgaggca attgcgtgtg acaaaa
//
LOCUS       DAORQA010000203         3263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7015_length_3263_cov_8.563903, whole genome shotgun sequence.
ACCESSION   DAORQA010000203 DAORQA010000000
VERSION     DAORQA010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7015_length_3263_cov_8.563903"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PK736_08105"
     CDS             complement(<1..749)
                     /locus_tag="PK736_08105"
                     /inference="COORDINATES: protein motif:HMM:NF015441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HRC33392.1"
                     /translation="MKVDPVLSRITIYPVKSLDGIEIQSALITEGGCLLHDREYALSD
                     EDGNFIIGKSNQLVHTLRSQVDFEKETISLRPESETAWRHFHLQKEKLALQSFLTDYF
                     GVKTVLLQNKNGRFIDIPDISGVTVVSTSSLKEVSTWFDNMDLEETRRRFRATLEIEG
                     VPAFWEDSLFTKAGSGIEFKVGDVTVFGMSPRARCVVPTRNTQTGEVLHGFPKSFARH
                     RAATLPQWSMLEQFGHHYHLSVDCYIPATEIG"
     gene            complement(997..3249)
                     /locus_tag="PK736_08110"
     CDS             complement(997..3249)
                     /locus_tag="PK736_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC33393.1"
                     /translation="MKNFVLFLLTIATITAKAQNRSISGSVRDRQTTEALPYSNVQLF
                     GTAIGSTTDNNGYFKLEIPKAHENGKLIATYLGYKTDTIQLTATKTIYKFNLNISAGT
                     MKEVVVSGTMKETSKLDSPIPVEVYNPVFFKKNPTANIFEALTMVNGVQPQLNCNVCS
                     TGDIHINGMEGPYTMVLIDGMPIVSSLSTVYGLAGIPNSMVKRIEIVKGPASALYGSE
                     AVGGLINIITKDPVTAPVLKVDIAGTTWNEYNADVTAKFKVKKASSLLGINGFYYNTI
                     HDINNDGFTDVTLQKRISIFNKWNFERKSGKPVSIAARYIYENRWGGQATWTPEFRGT
                     DSIYGESIYTNRAEVIGVYGLPLKHQNIRMEYSYNYHYQDSYYGTIKYKASQHTAFSQ
                     LLWDKTIDKHSLLFGVPFRFISYDDNSPATATADTLNPKNKPQLTILPGLFVQDEWTI
                     NAKLTTLVGMRYDHNNEHGNIFTPRLSFKFRPNTNHTIRLSGGNGYRVVNLFTEDHAA
                     LTGAREVVIAEKLKPEQSWNVNLNYVAQIDHKNGFIGIDMSTFYTYFTNKIVGDFNTD
                     PDKIIYDNLKGYAISKGATLNLDFAFTNGLKIILGGTLMDVYQIESDSAGVNVKMPQQ
                     FAPKFSSTFTASYTLSKLGLSFDLTGLVKSPMYLPVLPNDFRPEQSPWFCLVNLQATK
                     KVGNDIEFYGGVKNVLNFVPKNPIMRPHDPFDKDLTVNNPNGYTFDPSYNYAAVQGAR
                     IFFGVRWTLQ"
BASE COUNT          966 a          617 c          607 g         1073 t
ORIGIN      
        1 ccaatttccg ttgcaggaat gtaacaatca acagaaaggt gataatgatg gccaaattgc
       61 tcaagcatcg accattgcgg aagtgtagca gcacggtgtc gtgcaaaaga ttttggaaaa
      121 ccatgcaaca cttcgcccgt ttgtgtatta cgggtgggga caacacatct tgcacggggg
      181 ctcattccaa acacagtaac atcaccaact ttaaattcaa tgccgcttcc tgctttggta
      241 aataaactat cctcccaaaa tgcaggaaca ccttcaattt ctaaagttgc tctgaaacgc
      301 cttctggttt cttctaaatc catattatca aaccaagttg aaacttcttt taaactggaa
      361 gtagaaacaa cagttacacc actaatatcg ggaatgtcaa taaatcgtcc atttttattt
      421 tgcagtagta cagtctttac tccgaaataa tctgtgagga atgattgtaa tgccaatttt
      481 tctttctgca aatgaaaatg cctccatgca gtttcagatt cggggcgaag tgaaatggtt
      541 tccttttcaa aatcaacttg cgatcgtaaa gtatgaacca gttggtttga ttttccaata
      601 ataaaatttc catcctcatc gctaagcgca tattctctgt catgcaaaag acaaccaccc
      661 tccgttataa gggcactctg tatttcaatc ccatcaagcg attttacagg ataaatagtt
      721 attctactca aaacaggatc aaccttcata atgatttaaa aatgattttg catgtgacaa
      781 atgtcaatat atattttgat ttacgatagt ttatattttt gaggtagcag gagaaattat
      841 tttcgtttcg aaaagtaaga ttgatttatt gcaaaaggat gaaaagaaat gttgcccaca
      901 ttgatttgtt tacaacaaag gctaaaacaa aaattaataa tacaatggtt cataagccaa
      961 gatgaaaata aattgtgctt gcatatacgt tggtgtttat tgcaatgtcc accgcacacc
     1021 aaagaaaatt ctggctccct gcacagcagc ataattataa cttgggtcaa aagtataacc
     1081 attaggattg tttacagtta agtctttgtc gaacggatcg tgaggtcgca tgattggatt
     1141 tttaggaacg aagtttaaaa cgttttttac tccgccatag aactcaatat cgtttccaac
     1201 tttttttgta gcctgtaaat tcaccaagca aaaccatgga gattgctccg gtctgaaatc
     1261 gtttggaagc acgggtaagt acatggggct ttttactaaa ccggttaaat cgaaactcaa
     1321 acccaattta ctcaaagtat aacttgctgt aaaagttgat gaaaattttg gtgcaaattg
     1381 ttgtggcatt tttacgttca cacctgcact atcactttcg atttgataaa catccatcag
     1441 tgttcctccc aaaataattt tcagtccgtt tgtaaaagca aaatctaaat tcaaagtggc
     1501 tcctttcgaa atagcgtagc cttttaaatt gtcgtaaatt attttgtcag gatcggtatt
     1561 gaagtcgcca acaattttgt tggtgaagta ggtataaaaa gtgctcatgt caattccaat
     1621 aaacccattt ttatgatcta tttgcgcaac ataattcagg tttacattcc aactttgttc
     1681 gggttttaat ttttctgcta tcactacttc tcgtgctcct gtaagtgctg catggtcttc
     1741 ggtaaaaaga ttgactactc tatagccatt gccaccactt aagcgaatgg tgtggttggt
     1801 attggggcga aatttgaaac ttaatcgtgg agtaaaaata tttccatgtt cgttgttgtg
     1861 gtcgtagcgc ataccaacaa gtgttgtgag ttttgcattt attgtccatt cgtcttgcac
     1921 aaacaaaccg ggtaaaatag ttagctgcgg tttatttttc ggattcaaag tatcggctgt
     1981 ggcagttgcc ggtgagttat catcatacga aatgaaacga aaaggaacac caaaaagtaa
     2041 actgtgctta tcaatagttt tatcccaaag caattgagaa aaagccgtgt gttgcgatgc
     2101 cttatatttt atggttccat aatagctatc ctgataatgg tagttgtaag aatattccat
     2161 acgaatattt tgatgcttaa gcggcaaacc atacacacca attacttcgg cccggttggt
     2221 ataaatactc tcgccatata tactgtcggt tcctctaaat tcgggtgtcc aggtggcttg
     2281 tccgccccac cggttttcat aaatatatct tgctgcaatt gaaacaggct ttccactttt
     2341 gcgttcgaaa ttccatttat taaaaattga aattcttttt tgcagcgtta catctgtaaa
     2401 gccgtcatta tttatatcgt gaatggtgtt ataataaaaa ccattgatgc ctaacaacga
     2461 acttgctttt ttgactttaa attttgctgt cacatcagcg ttgtattcgt tccaggttgt
     2521 gcctgcaata tcaactttca aaacaggtgc tgtaacaggg tctttggtaa tgatgtttat
     2581 taaaccgcca acagcttcgc ttccatataa tgcagatgca gggcctttta caatttcaat
     2641 tcgctttacc atgctgttag gaattccagc caaaccataa actgttgaaa gggaagaaac
     2701 aattggcata ccatcaataa gcaccattgt atatgggcct tccatcccat taatgtgtat
     2761 gtcgcctgta ctgcatacat tgcaattgag ctgaggctgc acaccgttta ccattgtaag
     2821 ggcttcaaaa atatttgctg tcggattttt tttgaagaaa accggattat acacttcaac
     2881 aggaatggga ctatccaatt tagaagtttc tttcatggtg cctgaaacca ctacttcttt
     2941 cattgtgcct gcacttatat ttaagttgaa tttgtaaata gtttttgtgg ctgtgagttg
     3001 aatggtgtcg gttttatatc ctaaataagt agcaataagt tttccgtttt cgtgagcctt
     3061 tggtatttcc aatttgaaat aaccgttgtt gtcggtagtt gatcctatgg ccgttccaaa
     3121 taattgtaca ttgctgtatg gtaaagcttc ggtggtttgc ctgtccctta cgcttccact
     3181 tatgcttcgg ttttgggcct tagcagttat ggttgcgata gtaagtaaaa agagtacaaa
     3241 atttttcatc aacaatattt gtt
//
LOCUS       DAORQA010000204         3238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7071_length_3238_cov_5.446434, whole genome shotgun sequence.
ACCESSION   DAORQA010000204 DAORQA010000000
VERSION     DAORQA010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7071_length_3238_cov_5.446434"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PK736_08115"
     CDS             <1..821
                     /locus_tag="PK736_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33394.1"
                     /translation="ISTRINYLSCLNKIKAANIFSFMRLMVQLQIMHPMRALQSNNFD
                     ERTPNHFVLFCKHNITLYQYYYYIQSKTISIVGNPQYDAFNLKLKNETQEAPYIVYID
                     ADYLESDVYGWDKDFHQKVARNLEEFAVSEKIKMYIKLHPRSDEALWASYNLNSEYIT
                     ILKQGNFIELYLKAELILAHYTSLLMGLVPAQKNIVLLGWRPSNPQVAGTDFDKYDLM
                     HQSMYCEDLKEKYSYWIINNKTLLNQKQFHDFLDEFNSPFDGKATQRIIDIINK"
     gene            856..>1431
                     /locus_tag="PK736_08120"
     CDS             856..>1431
                     /locus_tag="PK736_08120"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33395.1"
                     /translation="MNIRKVFGTYMVATMYQAGLSFLIFPYLTHYLSTGDFGIIALVN
                     TYVTLIGAVAGMGCEGYFYINFFKAKPEEKSDYFLSSYYIPLISFLSIAIILVIFSVP
                     LAKIVELTPLAIVLIALLSLANFLYEQTLGLLIYNKKSLIYSIVIITKTSLEVLLTAF
                     AVIYFKEHWQGRIFAWLISIAIFGFWGLSYIY"
     assembly_gap    1432..1482
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1483..2134
                     /locus_tag="PK736_08125"
     CDS             <1483..2134
                     /locus_tag="PK736_08125"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRC33396.1"
                     /translation="LNKQITKESFRYGFPFVFHQLSKFSMNQSDRIFLSKLTTITQTG
                     IYNVAYQIGSVVNMVNLPFNNFFAPHMMEGLANPTPEKKKQIVKIAYSYILFLIVIVG
                     LTCVLAEIMYAFFINPNFIEGKKIVPLVGLAYFFWGIYLIFSGILQFEKKSKLISTIS
                     IVAFAINLILNYFMIKEYGYMGAAWATLITFAFQAASTAYYAHQTTHLPWLSFFRK"
     gene            complement(2145..>3238)
                     /locus_tag="PK736_08130"
     CDS             complement(2145..>3238)
                     /locus_tag="PK736_08130"
                     /inference="COORDINATES: protein motif:HMM:NF040307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6044 family protein"
                     /protein_id="HRC33397.1"
                     /translation="YIIQNRNFNFKYVFATMVLLLVYLIVEFPLVHSILFPTNYVSHR
                     EQFDITMYGEPVTIVSSMHKIIDALFVTQYHAGRFYTAAILISVVIVGLYKKLTKLHW
                     YLIAAIIVVAFTSVVNDYVLNAFQSQFKILKIWTSDRFFYLAPLLWFTLWAALLSQFN
                     WSQTKVRLIALFLFTIQFGATLITNSEMIRNVWQLMHKDFGEPDYNNFYATKLFDEIK
                     TDIGNDITSYRVGSVGLHPAVAQYNGFYTIDGYQNNYPLAYKSQFRKAISGELEKDQS
                     LKNYFDGWGSRAYLYSSEVLPNSLISKNQNTNHITNLQLSSESLKFLNAKYIFAALPI
                     QNADTSGLQLLKTYETPTSFYKIYVYKVM"
BASE COUNT         1089 a          558 c          519 g         1021 t
ORIGIN      
        1 gaataagtac gcgaataaat tatctcagct gtttaaataa aataaaagct gcgaatattt
       61 tttcgtttat gagattaatg gtgcagctgc aaataatgca tccaatgagg gctttgcaga
      121 gcaataattt tgacgagcgt acgcccaatc actttgttct gttttgtaaa cacaacatta
      181 ctttgtatca atactattat tatattcaat cgaaaaccat ttctatagtt ggcaatcctc
      241 agtacgatgc gtttaatttg aaattaaaaa acgaaacaca agaagcgcca tatattgtat
      301 acatagatgc cgattatctg gaaagcgatg tgtatggatg ggataaagac tttcatcaaa
      361 aagtggcacg caaccttgaa gaatttgccg tgagcgagaa aataaaaatg tatataaaac
      421 ttcatccccg cagcgatgag gctttgtggg caagttacaa tttgaacagt gaatatatta
      481 ccattctcaa acaaggaaac tttattgaac tttatcttaa agcagaatta atattggcac
      541 actacacctc gctgctaatg ggacttgtac cggcacaaaa aaacattgta ttgcttggtt
      601 ggcggccatc aaatccacaa gtagccggca ccgattttga caaatatgat ttgatgcatc
      661 agtcaatgta ctgtgaagat ttaaaagaaa aatattccta ttggataata aacaataaga
      721 ccttactaaa ccaaaaacag tttcatgatt tcttggatga atttaattct ccattcgatg
      781 gaaaagcaac acagcgaata atagatatta tcaataagta gaaatgtaat tacattgcac
      841 caataagtat tattgatgaa cataaggaaa gtattcggaa catatatggt agccacaatg
      901 tatcaggccg ggttatcgtt tctgatattt ccttacctga cacactacct aagtactggc
      961 gactttggaa ttattgcatt agtaaatact tatgtaacat taattggagc tgttgcaggc
     1021 atgggttgcg agggatattt ttacattaac tttttcaaag caaagcccga agaaaaaagc
     1081 gattacttct tatcatcata ttatattccg ttaatttcat ttttatcaat agcaataatt
     1141 cttgttattt tttcggtacc gctggccaaa atagttgagc tcacaccttt ggccattgtg
     1201 ttaatcgcat tattgagttt agctaacttt ctatatgagc aaactttggg actgctcatc
     1261 tacaataaaa aatctttgat ttatagtatt gtcatcatca caaaaaccag tctcgaagta
     1321 ttgttaacag catttgcagt tatctatttt aaagaacact ggcaaggccg catttttgca
     1381 tggcttataa gcattgccat tttcggcttt tggggtttaa gttatatata cnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnactcaaca agcaaatcac
     1501 aaaagaaagt ttccggtacg ggttcccctt tgtttttcac caactgtcca aattcagcat
     1561 gaaccaatct gacagaatat ttttatctaa acttacgacc atcactcaaa cgggaattta
     1621 caatgtagca tatcaaattg gaagtgttgt gaatatggtg aatctgccat tcaataattt
     1681 ttttgctcca catatgatgg aaggccttgc aaatccaact ccagagaaaa aaaaacaaat
     1741 tgtaaaaata gcttactcct atattttatt tctgattgta attgttggat taacttgtgt
     1801 acttgccgaa ataatgtatg ccttttttat caacccaaat tttattgaag gcaaaaaaat
     1861 agtcccactt gttggtttag cttatttctt ctggggtatt tatctcatat tctctggcat
     1921 attacaattc gaaaaaaaat cgaaattaat ttctaccatc agtattgttg cttttgcaat
     1981 taaccttata ctaaattatt ttatgataaa agaatatggt tacatgggcg ctgcatgggc
     2041 tacgctaatt acatttgcct ttcaagctgc aagcactgct tactatgcac atcaaactac
     2101 acatttgccc tggttgtcat ttttcagaaa atagtttttt aaaatcacat cactttataa
     2161 acataaattt tataaaacga tgttggcgtt tcgtaagtct ttaaaagttg taacccgctt
     2221 gtatctgcat tttggattgg caatgcagcg aaaatatatt ttgcatttaa aaatttcaaa
     2281 gattcactac tcaattgcaa attcgtaata tgatttgtgt tttgattttt actaatcagt
     2341 gaattaggca acacttctga tgagtataaa tacgcccggc ttccccaacc atcaaaataa
     2401 tttttaagtg attggtcctt ttccaattcc cctgatatag cttttcggaa ttgtgactta
     2461 tatgctaaag gataattatt ctggtaacca tcaattgtat aaaaaccatt gtactgcgca
     2521 acagccggat gcaatccaac cgaacccaca cgatatgatg taatatcatt tccaatgtca
     2581 gttttaattt catcaaacaa ttttgtagca tagaaattat tgtaatcggg ctctccaaag
     2641 tccttatgca tcaactgcca aacgtttctt atcatttcgg aatttgtaat taaggtggca
     2701 ccaaattgaa tagtgaacag aaacaaagca atcaatctta ccttagtttg actccagttg
     2761 aattgactta aaagagctgc ccaaagcgta aaccataaca gtggtgctaa ataaaaaaat
     2821 ctgtcggaag tccatatttt taaaatctta aattgcgatt gaaaggcatt caaaacataa
     2881 tcgtttacaa ccgaagtgaa agctactacg ataattgcag caattaaata ccaatgtagt
     2941 ttcgtcaatt ttttataaag tccaactatt accactgata ttaaaattgc agcagtataa
     3001 aacctgcctg catgatactg agtgacaaac aaggcatcga taattttatg catagaagaa
     3061 acaatggtca caggttcgcc atacattgtt atatcaaatt gctcgcggtg tgaaacataa
     3121 ttggtgggaa acaatataga atgaaccaac ggaaactcaa caattaaata cactaacaaa
     3181 agaaccatgg tagcaaaaac atatttaaaa ttgaagttgc ggttttgaat aatataga
//
LOCUS       DAORQA010000205         3230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7087_length_3230_cov_5.090394, whole genome shotgun sequence.
ACCESSION   DAORQA010000205 DAORQA010000000
VERSION     DAORQA010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7087_length_3230_cov_5.090394"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..>1485)
                     /gene="porU"
                     /locus_tag="PK736_08135"
     CDS             complement(74..>1485)
                     /gene="porU"
                     /locus_tag="PK736_08135"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HRC33398.1"
                     /translation="RGWKNANRLPIFVTATCEFSRFDDPGRTSAGELILINSENGGIG
                     LFTTTRVAYTNTNLDLNKNLIQSFFNFTNGKPRLGDIMRYTKALSAQDQNRNFCLLGD
                     PAVVPAFPEYKLSVTQINDQPAALFNDTLKALSNVKIKGVIKDASNNIITNYKGVVYP
                     TIYDKTQKITTLSNDPASPATQFLLRKNVLYKGKAEIKNGEFEFTFMVPKDIDYTVGL
                     GRISLYAQDGKTDAMGVYDSAKIGAINPSPPSDSKGPSVELFMNNENFNMGGTTDANP
                     RIYAVLNDEYGINTVGNGIGHDITATLDNQTQNIFTLNDYYQGDLNNSASGKVIYPLY
                     NIANGNHTLTFKVWDIFNNSSEAKTEFVVASEANIALNHVLNYPNPFTTNTRFMFDHN
                     QACNTLLVEIKVFTVSGKVVKQFNQIINCEGYHEGIQWDGRDDFGDRLGRGVYLYKVK
                     ISDVDGKSAEKIEKLVLLR"
     assembly_gap    1486..1495
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1496..>2289)
                     /locus_tag="PK736_08140"
     CDS             complement(<1496..>2289)
                     /locus_tag="PK736_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRC33399.1"
                     /translation="FIDEAEQLAKFHRDNDGLSTLVVTTEQVYNEFSSGAPDVSAIRD
                     MMRMFYKKSIGPQDMPKYLLLIGDGSYDNKNREYSTNNLILTWQSASGCSFTGSYVTD
                     DFFGLLDDDEGNVNSGEEVDIGIGRFPVFTNEQAQVCVDKVINYSTEPGYIVEEASCN
                     NGVRSPLGDWRNTICYVGDDEDSNTHLQHNEIMVSISQQLANEFTVDKIYFDAFQQIS
                     TPGGKRYPSVKDAITKRVERGSLLINYAGHGGETGWSEERVLEVSD"
     assembly_gap    2290..2299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2300..>3230)
                     /locus_tag="PK736_08145"
     CDS             complement(<2300..>3230)
                     /locus_tag="PK736_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33400.1"
                     /translation="GKFDKNDFIAFYGQGPVKWKLNTADNRFHHQLNLYSDSTFYFIN
                     IDGAAGKRILPQAGSSLSPSNTATTFDDYQFYEDERQNFIKSGREWFGKQFDVDPDQS
                     FKFNFPHLTKTVPVYVQSEFAGRTFNTNVFFDLSYNGTPINKLKVTSFFSNYLSDFCA
                     LASSIQGNSISKTFNATTDDVAIDVKFTRTSSNANGWLNYIEMNATRDLIFTGSQMFF
                     RNKESAGAGKVTNFIITTSSSNNIRLWDVTSHDNVFEQQFNSNGGNINFVVATDSLRE
                     YVLFSESEYKEPLFGRKVENQNIHAEAIPKMVIV"
BASE COUNT          917 a          666 c          576 g         1051 t
ORIGIN      
        1 ggtttgagga gactatcacc aactattttt gaccattata tcctaatttt caattcttaa
       61 ttccatcctc tgattatcgc agcaaaacaa gcttctcaat tttctctgca ctcttgccat
      121 ccacatccga tattttcact ttgtataagt acactcctcg tccgaggcgg tcgccaaagt
      181 catcgcggcc atcccattgt ataccttcgt gataaccttc gcaatttatt atttggttaa
      241 actgcttcac cactttgccc gaaacggtaa acacttttat ttcgacaagc agtgtattgc
      301 atgcctgatt gtgatcgaac ataaagcgtg tgttggtggt aaacgggttg ggataattga
      361 gcacatgatt gagtgcaata tttgcctctg atgctactac aaattccgtt ttagcttcac
      421 tcgaattatt aaatatatcc cacactttaa aagtaagcgt gtggttaccg tttgcaatat
      481 tgtacaaagg ataaattact ttaccacttg ccgaattgtt caagtcgccc tgataataat
      541 cgtttagagt aaatatattc tgcgtttgat tatcaagggt tgctgtaata tcgtggccaa
      601 taccgttgcc tacagtattg ataccatatt catcgtttaa cactgcatat atacgtgggt
      661 tggcatcggt agttccgccc atattaaagt tttcgttatt cataaacaac tctaccgatg
      721 ggcctttact atcggaaggt ggcgatggat taatggcccc aatttttgca ctgtcataca
      781 ctcccattgc atctgttttg ccatcttgcg catacaaact gatacggcca agtcccacag
      841 tataatcaat atctttgggt accatgaaag taaattcaaa ttcgccattt tttatttctg
      901 ctttaccttt atacaataca ttcttgcgca acaaaaattg tgtagcaggc gatgcaggat
      961 cattagataa tgttgttatt ttttgtgtct tgtcataaat agttggatac accacacctt
     1021 tgtaatttgt tataatatta ttggaggcat cttttattac tcctttaatt ttcacattgc
     1081 tcaaagcctt cagcgtatca ttaaacaggg cggcaggttg atcgtttatt tgagtaactg
     1141 ataatttata ttcggggaaa gcaggcacaa cggccgggtc gccaagcaaa caaaaattac
     1201 gattttgatc ctgtgccgaa agcgctttgg tatatcgcat tatatctcct aacctaggct
     1261 ttccattcgt aaaattgaaa aaactttgaa taagattttt attcaaatct aagtttgtat
     1321 ttgtatacgc aacacgcgta gtagtgaaca acccaatgcc gccattttcg ctgtttatta
     1381 aaattagttc accggccgaa gtacgccccg gatcatcgaa acgactgaac tcgcaagtgg
     1441 ctgttacgaa tatgggaaga cgattggcat ttttccaacc ccttannnnn nnnnncatcc
     1501 gatacttcta aaacccgttc ttcgctccaa ccggtttcac caccatggcc ggcatagttt
     1561 attaaaagtg aaccacgttc tactctttta gtaatagcat ctttcacgct tgggtagcgt
     1621 ttgccaccgg gagtacttat ttgctgaaat gcatcgaagt aaatcttatc gactgtaaat
     1681 tcattggcca gttgttggct aatggaaacc ataatttcat tgtgctgcaa atgagtgttt
     1741 gaatcttcgt catcgccaac atagcatatg gtattgcgcc aatcgccaag agggcttcgt
     1801 acaccattat tgcacgatgc ttcttcaact atgtatcccg gttcggtgga ataatttatc
     1861 actttatcaa cacacacctg tgcttgttca tttgtaaaaa caggaaaacg tccaatgcca
     1921 atatctactt cttcaccact atttacattg ccttcatcgt catcgagcaa accaaaaaaa
     1981 tcatcggtta catacgaacc tgtgaacgaa catccgctgg ccgattgcca tgtgagtatt
     2041 aaattattgg tagagtattc gcggttttta ttatcgtatg agccatcacc aattagcaac
     2101 aaatattttg gcatatcctg agggcctata gattttttgt aaaacatccg catcatatcg
     2161 cgtatggcgc ttacatccgg tgctcccgat gaaaattcat tatacacttg ttcggttgta
     2221 actaccaagg ttgataagcc atcattatcg cgatggaatt ttgccaactg ttcggcttca
     2281 tctataaatn nnnnnnnnna ctatcaccat ttttggaata gcttcggcat ggatattttg
     2341 gttttctact tttcttccaa agagtggctc tttgtattcg ctttcgctga acaaaacgta
     2401 ctcgcgtaaa ctatcggtag ctaccacaaa attaatattg cctccatttg agttaaactg
     2461 ttgttcgaaa acattgtcgt gtgatgtaac atcccacaag cgaatattat ttgacgatga
     2521 agtagtaata ataaaattag tgactttacc tgctccagcg ctttctttgt tgcgaaaaaa
     2581 catttgcgag ccggtaaaaa ttaaatcgcg tgtggcattc atttcaatat aattgagcca
     2641 tccatttgca tttgaactgg tgcgtgtaaa ctttacatct atagcaacat catctgtagt
     2701 ggcattaaaa gttttcgaaa tgctattgcc ttgtatggat gatgccaatg cgcaaaagtc
     2761 gctcaggtaa tttgaaaaga atgatgttac tttcaactta ttgataggtg taccgttgta
     2821 acttaaatcg aaaaaaacat tggtgttaaa tgtccggcct gcaaattcgc tttgtacgta
     2881 tacaggtacc gttttagtaa gatgtggaaa attaaacttg aaactttgat cgggatcaac
     2941 atcaaactgt tttccaaacc actcgcgccc cgattttata aaattctgac gctcgtcttc
     3001 ataaaactga taatcgtcaa atgtggtggc tgtattgcta ggagataaag atgaacctgc
     3061 ttgaggtaaa attcttttcc ctgcagcacc gtcaatattt ataaaataaa atgtactgtc
     3121 gctgtataaa ttcaattgat ggtgaaaacg attgtcagct gtattcaatt tccatttcac
     3181 agggccttgt ccataaaaag ctataaaatc atttttatca aactttccat
//
LOCUS       DAORQA010000206         3227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7097_length_3227_cov_6.996217, whole genome shotgun sequence.
ACCESSION   DAORQA010000206 DAORQA010000000
VERSION     DAORQA010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7097_length_3227_cov_6.996217"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1055)
                     /gene="dapA"
                     /locus_tag="PK736_08150"
     CDS             complement(180..1055)
                     /gene="dapA"
                     /locus_tag="PK736_08150"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRC33401.1"
                     /translation="MKSLKGSGVALITPFKKDGSIDFDGLTAIVNHMIKGKMDYIVIL
                     GTTGEAATLNAEERNEVIKHIIDTNKKKLPLVLGIGSNNTADVVHHLQTDNLKGINAV
                     LSVTPYYNKPNQQGLYTHYKTIAAASPLPLILYNVPGRTGVNMTAETQLHLAHECENI
                     IATKEASGKMEQVMEIIKSKPKNFTVISGDDNLTLPMIACGAEGVISVSANAFPRQMS
                     DLVRSAMLHQWEKAKKIHYSIFNITNMFFEEGSPAGVKECLAHLSITKNYVRLPLTNV
                     SENLSQRILNEIAGL"
     gene            complement(1042..1608)
                     /locus_tag="PK736_08155"
     CDS             complement(1042..1608)
                     /locus_tag="PK736_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33402.1"
                     /translation="MSVHKKKIQNRIADYLLQREIKHHKRNAKTVSFVHARTIGILYD
                     ATHEEDYETIKEYVKHFRDQKKEVLALGYVDLKEMPAMRFSKLGLDFFTFKNLNWVLK
                     PAHPMVDKFIHANFDILINLNIQKCFPLKYLAAITKASFKIGRYESRHQQYLDFMIKT
                     NESVTLKQFIELTNKYIKIIGNGTNQIA"
     gene            complement(2064..>3227)
                     /gene="ligA"
                     /locus_tag="PK736_08160"
     CDS             complement(2064..>3227)
                     /gene="ligA"
                     /locus_tag="PK736_08160"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRC33403.1"
                     /translation="VIKVNEYQNQQELGYTAKSPRWAIAYKYKAENASTQLLSVSYQV
                     GRTGAITPVANLQPVVLAGTVVKRASLHNADIIEKLGLYENDFVFVEKGGEIIPKVTG
                     VDLAKRAIDALPIKYITHCPECGTELIRQEGESAHYCPNETGCKPQIVGRIEHFASRK
                     AMNIDGLGSETVELLVENKLVTNIADLYDLKKEDLLALDRFAEKSATNLIEGIAASKK
                     IPFEKVLFAIGIRYVGDTVAKKLALQFNNIENLQAADNAALVATPEIGEKIAISVQQY
                     FAEEKNLSALQRLKNAGLQFQISQEQLALRGDKLKGLIIVATGTLAHYKRDEIKAVIE
                     QNGAKAASGVSSKTSFVIAGTDPGQSKIDKANELKIKIISEEEFMEMLDGKGL"
BASE COUNT          863 a          654 c          570 g         1140 t
ORIGIN      
        1 agaacaatcc tcaccgcaaa agaaactaag aactaagaac aatcctcacc gcaaaagaaa
       61 ctaagaacta agaacaatcc tcaccgcaaa agaaactaag aactaagaac aatcctcacc
      121 gcaaaagaaa ctaagaacta agaacaatcc tcaccgcaaa cgaaactaag aactaagaat
      181 tacagccccg caatttcatt gagtattctc tgcgataaat tttcgctcac attggttaaa
      241 ggcaggcgca cataattttt tgtgatgctt agatgtgcca aacattcttt tacgcccgct
      301 ggcgaacctt cttcgaaaaa catatttgta atattgaata ttgagtaatg tattttttta
      361 gccttctccc attggtgcaa catagcactg cgaacaaggt cactcatttg ccggggaaat
      421 gcattggccg atacacttat tactccttcg gcaccacaag caatcatagg cagtgtaagg
      481 ttatcatcgc ccgatataac ggtaaaattt ttcggcttcg attttataat ttccattacc
      541 tgttccattt tgccgctggc ttctttggtg gcaattatat tttcgcactc gtgtgcaaga
      601 tgtaattgtg tttcggcagt catgtttact cctgtgcggc ccggtacatt gtatagtata
      661 agcggaaggg gagaggcagc cgctattgtt ttatagtgcg tatacagtcc ctgttgattc
      721 ggcttgttat agtatggtgt tacggaaagt acagcattta tgcctttaag attatccgtt
      781 tgtaaatgat gaaccacatc ggctgtattg ttgctgccaa tacctaatac cagaggcagc
      841 ttttttttat ttgtgtcgat gatgtgtttt ataacttcgt ttctttcttc ggcatttaac
      901 gtggcagctt ctcccgttgt gcccagtatt actatgtaat ccatcttgcc ttttatcata
      961 tggtttacaa tagctgtgag gccatcgaaa tcgatgctgc catctttttt gaatggcgta
     1021 atgagcgcca cgcccgatcc tttaagcgat ttgattggtg ccattgccaa ttatttttat
     1081 gtatttattt gttaactcta taaattgttt cagtgtaacg ctttcgtttg ttttaatcat
     1141 aaaatcgaga tactgctgat ggcgactttc gtagcggcct atcttgaaac ttgctttggt
     1201 aatagctgcc aaatatttaa gagggaaaca tttttgaatg ttcagattaa taagaatgtc
     1261 gaaatttgca tgtataaatt tatcaaccat agggtgtgca ggttttagta cccaattcaa
     1321 atttttaaag gtgaaaaaat ctaaacccag tttactaaac cgcatggcgg gcatttcttt
     1381 taagtctaca taacccagtg caagcacttc ctttttttgg tcgcgaaagt gctttacata
     1441 ttccttaata gtttcataat cctcttcgtg tgtggcatca tatagtatgc ctatagtacg
     1501 tgcgtgcaca aacgatactg ttttggcatt acgcttatga tgttttattt cgcgttgcag
     1561 taaataatct gcaatccggt tttgtatttt tttcttgtgt accgacatgg ttttggttta
     1621 tctttctact cgcaattcga ctttgctaaa ctaattatta tttttatttt tagttcactc
     1681 tttttagttt tttaattctt agtttttaat tcttagtata tagtcccgac acttcggtcg
     1741 ggatgtcgac cagtgtgtca gcatttatta ttttttgttt tggctactag ataatagttt
     1801 caaattatgg ttttttgttt ttggctccaa cattcggttg gtgctggaat ttaaagtcaa
     1861 aaaaaggtga atgtggaatt cggtgaaaat tatttgctat ctgcaaatct gcctgtttgt
     1921 tgaaaaatta caagcataat tttaatacac catttattac ggcagtgatg cttcgttact
     1981 cagcattgca aaacagtaac atcaaaatat cgcaagaata aacaacttaa caactcaata
     2041 accaataaca atgcaaccaa caactacaat cccttcccat caagcatttc cataaactcc
     2101 tcttcgctaa ttatttttat tttcaattcg ttggctttat caattttact ttggcccgga
     2161 tcagtaccgg caattacaaa cgatgttttg ctgctaactc cacttgcagc ctttgcacca
     2221 ttttgctcta tcaccgcttt tatttcatcg cgcttataat gtgcaagtgt accggttgca
     2281 acaataatta atcctttcaa tttatcgcca cgcaaagcaa gttgttcctg acttatttga
     2341 aattgcaaac cggcattttt taatctttgc aatgcgctca agtttttttc ttcagcaaaa
     2401 tattgttgca cactgatagc aattttttct cctatttcgg gtgtggcaac caatgcagca
     2461 ttatcggctg cttgcaaatt ttctatgtta ttaaattgca gtgcgagttt tttggcaact
     2521 gtatcgccca catatcttat tccaattgca aacaaaactt tttcgaaagg aatttttttc
     2581 gatgccgcaa taccttctat taaattggtt gccgattttt cggcaaagcg atcgagtgcg
     2641 agtaaatctt ctttttttaa atcatataaa tctgcaatgt tggttaccag tttattttct
     2701 accagtaact ccactgtttc gcttccaagg ccatctatgt tcatggcttt gcgtgatgca
     2761 aaatgttcta tgcggccaac tatttgcggt ttgcaacctg tttcgttggg gcagtagtgc
     2821 gcactttcac cttcttgccg tatcagttcc gtgccacatt cagggcaatg tgttatgtat
     2881 tttattggta aggcatctat tgctcttttt gctaaatcta caccggttac tttgggaata
     2941 atttctcctc ctttttctac aaacacaaaa tcgttttcgt acaaaccaag tttttcaatt
     3001 atatcggcat tgtgcaacga tgctcttttt actactgtac ctgcaagcac cacaggttgc
     3061 aaatttgcta ccggagttat ggcacctgtg cggcctacct gatacgatac gcttaataat
     3121 tgtgtggatg cattttctgc tttatactta taggcaattg cccaacgtgg cgacttggca
     3181 gtatatccta actcctgttg gttttggtat tcatttactt ttataac
//
LOCUS       DAORQA010000207         3219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7115_length_3219_cov_14.331226, whole genome shotgun sequence.
ACCESSION   DAORQA010000207 DAORQA010000000
VERSION     DAORQA010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7115_length_3219_cov_14.331226"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3219
                     /locus_tag="PK736_08165"
     CDS             <1..>3219
                     /locus_tag="PK736_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33404.1"
                     /translation="SGNIVSNCNISNYFSATLATNGILLTATGNSGWSITNNRLFQTA
                     NRTYTTGNAHNGISVQSGSGYIIIGNTIGFANSGGTLTTNMVGAAAPISGFPGNYAAV
                     VGIATTYTGINATFTSGGINSLIQSNIIAGFALFTSTGGGAAFTAINVTTGNVTIGGA
                     SAVLGNTIGSTSGLSSIYLATTANGGAFFGIRTSPAAGNTATVQNNTIGAVDAIGTTA
                     LLTGSFVGLDGFGAGNIEHINNTIGNATADNIRTGYTTLSGVTATNLSNTGIFVAAGT
                     TGTTATMSAIRSTNSGQSYNASFNTIRGLAMNATVSTALGITSSGAITGAVPSVTINN
                     NAIGTSGLGWMRYVAANSGALTGISMTNTVAILHSIQSNDFRGIIHNVASTNSLTFIN
                     LTGATANGNAATISDNTFTNLNVNTSGSVTFISHSYVVSSSGRQTINNNAIVTAFNKG
                     TAGGTITLATSGSSSVAGAIINHTNNNFSNITVTGATIIAGWISSDGGTANKTYSNNT
                     FSNWIGGTSSITAMSCNFGGGAGGNGNVLTNNTITNITGQGAITGIVLGASGTTHSAI
                     TNTITGLSSTGVGGNVTGITSAAPTAGNINGNIISNLSSTAASGIVTGIAVTGGTLTT
                     VNDNRISGLSGTVLATTSPISNGISVSGGTTININRNKICDIAENGVSTLNLVNGISL
                     SGGTTVTAANNIIGDLRAPVSNNADAIRGIAVTSTTAATSYNLYNNTVYLNASSTGTP
                     FGTTGVFHAANATATTAKLDLRNNIIVNNSTPAGAGISSALRRSVAAILGNYAITSNN
                     NMYYAGVPSAVNAILNDNGTVYGTAAAAFAFGPVGTAGSFQNFVTASRESASFTEVVS
                     NAPGVYFQSVTCSDPTFLHIVNGLTTQLESSGISPNIVTFATDYDGDIRAGSAGYAGT
                     GTAPDVGADEFSGTTPAPSITAVAITPSGSQCVASNRLVSATVTTVIGAITGVVINYS
                     YNGVPQTAITMTNTSGNIWEGTIPAAAPTNATVTWSILATNSLSITSTFNGISYSDDP
                     LLSSCSFLTSSSSTVCSGSPATLTASVFSPSSNVQTYVNHAGA"
BASE COUNT          956 a          662 c          660 g          941 t
ORIGIN      
        1 cagtggcaac attgtttcta attgtaatat ctcaaactac tttagtgcaa ccttagcaac
       61 caatggaatt ttattgacag ccacaggaaa ctcaggatgg tcaattacta acaaccgttt
      121 gtttcaaaca gccaacagaa cttatacaac cggtaatgct cataatggaa taagtgtaca
      181 gtcaggttca ggttatatta ttatcggaaa cactatcggt tttgcgaatt caggcggtac
      241 attaacaaca aatatggttg gtgctgctgc acctatttcc ggatttccgg gtaattacgc
      301 agccgtagtc ggtattgcta ctacttacac cggcataaat gcaacattta catcaggagg
      361 tattaattca ttaatacaat ccaacatcat tgcaggattt gctttgttca cctcaacggg
      421 tgggggtgct gcttttaccg cgataaatgt tacaacagga aatgttacta ttggaggcgc
      481 ttcagctgtt ttaggaaaca ctattggttc aacatcaggg ctaagctcaa tttatctggc
      541 tactacagct aatggtggag cattttttgg tatcagaaca tcgcctgcag caggtaatac
      601 agccacagtt caaaacaaca ccattggggc agttgatgct attggaacca cagcactttt
      661 aactggttcc tttgtgggac ttgatggttt tggagcagga aacattgaac acattaataa
      721 cacgatagga aatgcaacag cggataatat tcgcaccggt tataccacat taagcggggt
      781 tacagctaca aatttatcca atacagggat ttttgttgca gcaggtacta caggaacaac
      841 cgccaccatg tcggcaatca gaagcaccaa ttcaggtcaa agctataatg catcttttaa
      901 tactatcaga ggtttggcaa tgaatgccac tgtaagtaca gcattaggaa ttacatcttc
      961 tggcgcaatt actggtgcag taccttcagt aaccatcaac aataatgcta taggtacatc
     1021 aggtcttgga tggatgaggt atgtagccgc taactcaggt gctttaacag gtatctcaat
     1081 gactaatacc gttgctattt tacattctat acaatcaaac gatttcagag gcatcattca
     1141 taatgttgct agtactaact ccttaacatt cattaatctt accggagcta cagctaatgg
     1201 taatgcagca actatatccg ataacacttt tactaattta aatgtcaata caagtggtag
     1261 tgttaccttt atttcgcaca gttatgtagt ctcgtcaagt ggaaggcaaa ccataaataa
     1321 caatgcaatt gttacagctt ttaacaaagg cacagcgggt ggcaccataa cgcttgcaac
     1381 ctctggatct tcatctgttg caggtgctat tattaatcac acgaataata atttctccaa
     1441 tattactgta accggtgcta caataattgc aggttggatt agctcggatg gtggcacagc
     1501 aaataaaaca tatagtaaca atacttttag taattggatt ggaggaacaa gttctattac
     1561 tgccatgagt tgtaattttg gcgggggtgc aggtggtaat ggaaatgtat tgactaataa
     1621 tacaattaca aacattaccg gacaaggtgc tattactggt atagtacttg gtgccagtgg
     1681 aaccacacat agtgcaatca caaataccat cacgggtttg tcatcaactg gagtgggtgg
     1741 taatgttacc ggtattacct ctgcagcacc tacagccgga aatattaatg gcaatataat
     1801 aagtaatttg tcatctaccg ctgcttcggg tattgtaaca ggaattgccg ttacaggcgg
     1861 tacgcttacc accgtaaatg ataatcgcat ttcgggttta tcgggaacgg tattagctac
     1921 aacctcaccg atttccaacg gtatttcggt gagtggtgga acaaccataa atatcaaccg
     1981 caataagatt tgcgatattg ctgaaaatgg cgtttcaact cttaatttag taaatggtat
     2041 ttcgctttca ggtggaacta ctgttactgc tgccaacaat ataattggtg atttgcgggc
     2101 acctgtatca aataatgccg atgccataag gggcattgca gtaaccagta caaccgcagc
     2161 aacatcatac aatttatata acaatacagt ttatctaaat gccagttcta ccggtactcc
     2221 ttttggaacg accggtgttt tccatgcagc caatgcaaca gcaacaactg ccaaacttga
     2281 tttaagaaat aatataattg taaataattc gacccctgcc ggtgcaggta tttcatcggc
     2341 attaagaagg tcggtggctg ccatcttggg taattatgcc atcacctcga acaataatat
     2401 gtattatgct ggtgtaccaa gtgcagtgaa tgccatttta aatgataacg gaacagttta
     2461 tggtaccgct gctgctgcat ttgcttttgg cccggtaggc accgcgggtt ctttccagaa
     2521 ttttgttacg gcctcacgcg agtcagcatc atttaccgaa gtggtttcta atgcacctgg
     2581 tgtttatttt caaagtgtta catgtagtga cccaaccttc cttcatatag ttaatggatt
     2641 aactactcaa cttgaaagta gtggtataag ccctaacatt gtaacgtttg caaccgacta
     2701 cgatggcgac atacgtgcag gaagtgcagg ctatgccggc acaggaacag cacctgatgt
     2761 gggtgcagat gagttttcgg gcaccactcc cgcaccttca attactgctg tagccattac
     2821 accaagcggt tctcaatgtg tggcttccaa tcgtttagta tcggccacag taactactgt
     2881 cataggtgca attacgggtg tagtgattaa ctatagctat aatggtgttc cgcaaacagc
     2941 aataacaatg actaatacaa gcggcaatat ttgggaaggt actattcctg ctgctgcacc
     3001 aacaaatgct actgttacat ggagcatttt agctaccaat tcattgtcta taacaagtac
     3061 ttttaatggc atatcatata gcgatgaccc tttgttaagt tcatgcagtt ttttaacatc
     3121 tagcagttca acagtatgtt caggttcgcc tgcaacatta acagcaagtg tttttagccc
     3181 ttcttccaat gttcaaactt atgttaatca tgccggagc
//
LOCUS       DAORQA010000208         3203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7154_length_3203_cov_10.486023, whole genome shotgun sequence.
ACCESSION   DAORQA010000208 DAORQA010000000
VERSION     DAORQA010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7154_length_3203_cov_10.486023"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..2155)
                     /locus_tag="PK736_08170"
     CDS             complement(14..2155)
                     /locus_tag="PK736_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC33405.1"
                     /translation="MIANTYTTATTPKLFLAAILFCSAFTFYPTNLFAQSHCDNSDFI
                     NGNFDNWLGSIGFCCPINTGTNVLVQGRHTIMSGTGMDLNTNNAIPVVAPGDLYSARL
                     GNDIAGSEAEKLSFSFNVTTDRELFVYKYAVVLEDPGHGAADQPRFEIRVFDSLNQQI
                     GCGFYNVVASANIAGFTNIHNQFGADIVYKDWSSVGVDLSSYSGQNITIEFATGDCAF
                     GGHYGYAYISAYCSPLSLEAYYCDGFYDATIQAPEGFYAYQWSSGETTSSIHVQNPVN
                     GSSYSCVLTSFTGCTVAISSILTPTKVTADFDAQSRCIDHVGFLDKTIVSNSTVKKWN
                     WNFGDNTFDSVQHPTHTYTTIGVYDVQLLVTTKGGCVDSITKQITVHDYPEIKFTHTL
                     GCVGIPLHFENQSVASTSPNTGLVWNINNVLYTNASSAIYTHNTTDSFPATLIVTNDA
                     GCTDSITEIIPMLKSPRPAFDFQNNCALSPLQFFDKTVSDFSIIKYVWDLGDGKNSHE
                     QNPIHKYTKPGNYLITLLAENDKHCVGDTSLVIKVLDTLIADFIYTPDKITTLTADVS
                     FINYSLKNLTFYHWDFGDGYTSDYFNETHRYAEAGRYPVQLWVTNNDGCRDTMSKEIL
                     VTPMPGIHIPNTFTPNDDAKNEMFFPLSENIKDYRILIYNRWGQVVFNGEDNKPWDGK
                     TSNEGVYVYKISGTDFFNQPFEKYGEVTLLK"
     gene            2607..>3203
                     /locus_tag="PK736_08175"
     CDS             2607..>3203
                     /locus_tag="PK736_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33406.1"
                     /translation="MSKYWMFGDSAGIEFNGTNTPNVINSHANGRGSSVSIADTNGAL
                     LFYVTTNGDNYRTVPMNKFNDTLFNGVDITGRNLYNDLTIVPFPNHANQFILFHVSVS
                     AFNDTLYYSIIDMQCNNNQGCVIAKNVPFDIYRSGDCLTAIKHGNGRDWWLLSKYSDV
                     NTPMNNRFFVYSVDSSGINLQSTQDFNDATDGDFQKIIV"
BASE COUNT         1002 a          594 c          606 g         1001 t
ORIGIN      
        1 atcctatggt ttcttatttt aataaagtaa cctcaccata cttttcgaat ggctgattga
       61 aaaaatcggt gcccgaaatt ttgtatacat acacaccttc gttagaagtt ttgccatccc
      121 acggtttgtt atcttcgcca ttaaatacaa cttgccccca acggttatat atcaggatgc
      181 gataatcttt tatgttttcg ctcagaggaa aaaacatttc gttttttgca tcgtcatttg
      241 gtgtaaacgt attggggatg tggatgccag gcatgggtgt taccaaaatt tctttgctca
      301 tggtatcgcg acaaccatcg ttatttgtta cccacagttg aacaggataa cggcccgctt
      361 cggcatatcg atgcgtttcg ttaaagtaat cggaagtata tccatcgcca aaatcccaat
      421 gatagaaagt aagatttttc aacgaataat ttataaacga aacatcggct gtgagcgtag
      481 taattttatc gggtgtgtaa ataaaatctg caattagtgt atcaagaact ttaataacca
      541 aacttgtatc gcccacacaa tgtttatcgt tttcggcaag aagtgtaata aggtaattgc
      601 ccggtttggt atatttgtga attgggtttt gctcatgact atttttacca tcgcccaagt
      661 cccacacata ttttataatg gaaaaatcgc tcacagtttt gtcaaaaaat tgtagcggac
      721 tcagcgcaca attattttga aaatcgaagg cgggccgtgg actttttagc atgggaatta
      781 tttcggtaat ggaatcggta catccggcat cgttcgttac aatgagcgta gccggaaacg
      841 aatcggttgt gttatgtgtg taaatggcac tgcttgcatt tgtataaaga acattattta
      901 tattccacac taagcctgtg ttgggcgatg tagaagcaac actttggttt tcgaaatgta
      961 aaggaatacc aacacatccc agtgtatgcg taaattttat ttcgggataa tcgtgcaccg
     1021 taatttgttt tgtgatagaa tcaacacaac cgcctttagt tgtaactaaa agttgcacat
     1081 cgtatacacc tattgttgta taagtatgcg taggatgctg cacactatca aaagtattgt
     1141 cgccaaaatt ccaattccat ttttttacgg tgctattgct cacaatagtt ttgtctaaaa
     1201 aacctacatg atcaatacac cgagattgtg catcgaaatc ggcagtaact tttgttggcg
     1261 tgagtatact gctgatggca acagtacatc cggtaaagct tgtaagcaca cacgaatacg
     1321 aactgccatt tacaggattt tgaacatgaa tgctgctggt ggtttcgccc gaactccatt
     1381 gataggcata aaaaccctcg ggtgcttgta tggtggcatc gtaaaatcca tcacaataat
     1441 atgcttctaa cgaaagtgga ctgcaatagg ctgatatgta tgcataacca taatgtccac
     1501 caaatgcaca atcgcccgtt gcaaattcta tggtaatatt ttgcccgcta tacgatgata
     1561 aatccacacc taccgatgac caatctttgt aaactatatc ggcaccaaat tgattgtgaa
     1621 tatttgtaaa ccctgcaatg tttgccgatg ctaccacatt ataaaaacca catcctattt
     1681 gttgattgag cgaatcgaaa acacgaattt cgaaacgagg ttgatcggca gcaccatgcc
     1741 cggggtcttc gagcactacg gcatatttat atacaaacaa ttcgcggtcg gtagttacat
     1801 taaaactaaa cgataatttt tcggcttccg atcctgcaat atcattaccc aaccttgccg
     1861 aatataaatc accgggagct accaccggta ttgcattgtt ggtatttaaa tccatgcctg
     1921 tgccgctcat tatagtatgc cgcccttgca ccaacacatt tgtgcccgtg ttgataggac
     1981 aacaaaaacc tatagaacca agccagttat cgaaattgcc atttataaaa tccgaattat
     2041 cgcaatggct ttgtgcaaat aaatttgtag gataaaatgt aaacgcactg caaaataata
     2101 ttgcggcaag aaataattta ggggtggtgg cggtagtgta agtgtttgct atcatctgaa
     2161 tttgttttta tccattcaga tataaaagca ttattttatt ttgctatttt attttccata
     2221 cccttagcaa tactcaacat acttctaatt ctgaatttct ttttagtaac tgtgagtgac
     2281 tggattaatt caatagcgcg ctgatttaat tgattcaaca tctgtgccaa gatgaatggg
     2341 gtgtgaatca aaaaataatt gtgctttaaa tttaaagcag aaaacacatg atgatgcatt
     2401 cattacatta gcacatgtat aatatgttgt acatttgtaa tcgtagaaaa aaatgttgag
     2461 taaaacattt gtgaaggaat aaatagaatg tatcaaaatc tcaaaccaaa gcgtttgtca
     2521 gaggcgcaaa ccaaagacag aaaataatga aactctttat tttattttta ctgctctgct
     2581 tgcaattatc aagcaaagct cagaacatga gtaaatactg gatgtttggc gattctgcgg
     2641 gtattgaatt taacggaaca aatacgccca atgtaataaa ttcacatgca aacgggaggg
     2701 gtagtagtgt ttctattgca gatacaaatg gcgctctttt attttatgta actacaaatg
     2761 gagataatta ccgcacggtg ccgatgaata agtttaacga cactttattt aatggcgtgg
     2821 atataacagg tcgtaacttg tataatgatt taactatcgt accatttcca aatcatgcaa
     2881 atcaatttat tttatttcat gtttcagttt cggcattcaa cgatacttta tattattcaa
     2941 taattgacat gcaatgcaat aacaaccaag gctgcgtaat tgcgaaaaat gtaccattcg
     3001 atatttatcg ttcaggcgat tgcctcacgg ccataaaaca tggcaatggg cgcgattggt
     3061 ggttgcttag taaatatagc gatgtgaaca cacctatgaa caaccgcttt tttgtttatt
     3121 cagttgatag tagcggtatt aatttacaaa gcacacaaga tttcaatgat gcaacagatg
     3181 gtgattttca aaaaataatt gtg
//
LOCUS       DAORQA010000209         3156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7272_length_3156_cov_4.976782, whole genome shotgun sequence.
ACCESSION   DAORQA010000209 DAORQA010000000
VERSION     DAORQA010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7272_length_3156_cov_4.976782"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..>3156
                     /locus_tag="PK736_08180"
     CDS             58..>3156
                     /locus_tag="PK736_08180"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33407.1"
                     /translation="MSISTPIPADYMGLNSANTVRTDADFPYIDDNWLVDSLDKMSIG
                     NLRFPPGTYGNFWDWHLGSIKDESKLPHDDDFLLDLGNLKYKDNSFDRLKNTIEKTGA
                     AHIYAMNILNSDKYYEAAGLYYLNSLNLTPKYIEMGDELYNDFDSYVGSFPTSKSYAD
                     KVNDWSSYIKGLPGLSNIKICAVGATNRSSQPDYSRRNTWNKEVCSFLSNDVDAIALH
                     FYLGANLRGQPLTTANMNDFMAAPFIGFSKLQEELTTVKAAGKEAWITEFNIYDRNTC
                     VNGSMAHALYLGSLSLCLLEDPNITKYCAHALTGNATWALLFNNSTGFETESYYYPGI
                     SCNPNPPATVAHDFTAPGTVIKMIGNAMSNATSRKKLSFPACPKIGDDYDAVYGWMFD
                     GPLAEEAIILNLSATQATININSNQWNLSNATYEQVYPGSGGVMDFVTGSPTKGSVAG
                     RPRTTGVLSAGQTLILKPYSITRISKKKNTITLKAVDSKICSGTTTTLIANGGTSYTF
                     NGSGLVGLVSKNVIKFSANVTAQKTYTITVTDINGASATASITVYPSPQLTVGISKSV
                     VCAKGTPVNLTASLTGGNSNNVASYLWIPNQDITNPNSGNTVAKPSKTTTYTVYATDG
                     QCYTAYDSVKVVVSNQAPPATFYNVCSNALPYQIKIDNPQSGFTYKWYEGNNLVYTGT
                     SFSANPQTLVTKYKVEMVSNSNSGCIDSTYATLNNYKCCTAPVPSLNFQPGAHMKDIR
                     ETIFDYCELTGKGVADEFGVSNLQEEILFNGVIYVDANYSFTNCKNIKFGEDAELRLI
                     DFAYDLEFHTCSLSNATCATSMWKGISITEFDQQVVLDNCYITNAKCVINAERDPILY
                     ITNTTFDANYVSICLNKNDKEFRGTIEGCTFTQSHSMMPPFLGKKPCAHIKINNVSSM
                     KVGNPLGAINNFEKAEYGIYAINSNLTVYNNLFNHIRSSDQINEGKASCIYLENSNGY
                     GNLTDINQLWKLYNINAGKSDNSPNYNNVFKNSRNGIYGVNCGINSYYNQFDNCKRGI
                     KVI"
BASE COUNT         1047 a          577 c          583 g          949 t
ORIGIN      
        1 attctggcgc tatttatatg cgcaaccaac tcgaatggtc aaaacattaa cgttggaatg
       61 tcaatttcaa cacccatacc cgcagattat atgggtctga atagtgccaa tacagtgcgc
      121 actgatgctg attttcccta cattgatgac aattggttag ttgattcatt ggataagatg
      181 agcattggaa acttgcgatt cccaccgggt acatatggga atttttggga ttggcattta
      241 ggctcaatta aggatgaatc aaagttaccc catgatgacg attttctcct cgatttgggt
      301 aatctcaaat ataaagacaa cagttttgac cgtttaaaga atacaattga aaaaaccggt
      361 gcagctcata tttatgcaat gaacatactt aattctgaca aatactatga agcagcagga
      421 ttgtattatc taaattcttt aaatcttaca cccaaatata ttgaaatggg tgatgagttg
      481 tataatgatt ttgattcata tgttgggtct tttccaacat cgaaaagcta tgctgacaaa
      541 gtgaatgact ggtcttcata tattaaaggt ttgccaggac tgagcaatat aaagatatgt
      601 gccgtaggtg caacaaatcg ctcatcacag ccagattaca gccgcagaaa tacttggaac
      661 aaagaagttt gcagttttct ttcaaatgat gttgacgcta tagctttaca tttttatctg
      721 ggagcaaatt tgcgaggcca accattgaca actgccaata tgaatgattt tatggctgcg
      781 ccttttatcg ggttttcgaa acttcaagaa gaactcacca ctgtaaaagc tgcaggaaaa
      841 gaagcatgga tcacagagtt taatatttat gatagaaata cttgtgtaaa tggttcaatg
      901 gctcatgccc tttaccttgg tagtttatcg ctttgcttgt tggaagatcc caatattaca
      961 aagtattgtg ctcacgccct tacaggaaat gctacttggg cattattgtt taataatagc
     1021 acaggtttcg aaacggaatc ttattactat cctggtattt cctgcaatcc aaacccacca
     1081 gcaactgtgg cccacgattt tactgcaccg ggaactgtga taaaaatgat aggaaatgca
     1141 atgtcaaacg caacgtcacg taaaaaatta tcattccctg cttgccctaa aataggggat
     1201 gattacgatg cagtttatgg atggatgttc gatggcccat tggcggaaga agcaataatt
     1261 ttgaaccttt ctgccactca agcaacaatc aacattaatt ccaaccaatg gaacctaagt
     1321 aatgctactt acgagcaagt ttatccgggt agtgggggcg taatggattt tgtaacagga
     1381 agcccaacca aaggtagtgt tgccggacgc ccaagaacaa ccggtgtttt gtctgccggc
     1441 caaaccttga tattgaagcc atattctatt acacgtattt caaagaagaa aaacactatc
     1501 acactgaaag ccgttgatag caaaatttgt tctggcacaa ccacaacttt aattgcaaat
     1561 ggtggtacat catatacatt taacgggtct ggacttgtag gtttagtttc aaaaaatgta
     1621 ataaaatttt cggcaaatgt aactgcgcaa aaaacctaca caataacggt aacagatatt
     1681 aatggtgctt cggctactgc aagcattaca gtatatccaa gtccgcagtt aactgttggg
     1741 atatcaaaat ctgtagtatg cgcaaaagga acacctgtta accttaccgc ttcattaaca
     1801 ggtggaaaca gtaataatgt tgcttcatac ctttggatac ctaatcagga tattacaaat
     1861 ccaaacagtg gcaataccgt tgcaaagcca agcaaaacaa caacgtacac agtttatgct
     1921 accgatggtc aatgctatac tgcctatgac tcagtaaaag ttgttgtttc aaatcaagca
     1981 cctcccgcta cattttacaa tgtttgctca aatgcattac cataccaaat aaaaatagac
     2041 aatccccaat ctggttttac ttataaatgg tatgaaggaa acaatcttgt atatacggga
     2101 acttcttttt cagctaatcc acaaacccta gttacaaaat ataaggttga aatggtaagt
     2161 aattcaaact caggctgtat tgacagtact tatgcaacat tgaataatta taagtgctgt
     2221 acggcacctg ttccatcact taactttcaa cctggtgctc atatgaagga tattagagaa
     2281 accatttttg attattgtga attaaccggc aaaggggttg ctgatgaatt cggtgtatcg
     2341 aatctgcaag aagagatatt atttaatggt gttatttatg ttgatgctaa ttattctttt
     2401 acaaattgta agaatatcaa atttggcgaa gatgctgaac ttagacttat tgattttgct
     2461 tacgacttgg aatttcacac ttgctctctt tctaatgcca cttgcgccac aagcatgtgg
     2521 aagggcattt cgatcacaga attcgatcag caggttgttt tagacaactg ctatattaca
     2581 aatgcgaaat gtgtaattaa tgccgaaaga gatcctatac tttatatcac caatactaca
     2641 ttcgatgcaa attatgtttc aatatgtttg aacaagaatg acaaagaatt tcgtggtacc
     2701 atagaaggtt gcaccttcac tcaaagtcat tcaatgatgc caccattttt aggtaaaaaa
     2761 ccatgtgctc atattaagat aaacaatgta tcgagtatga aagttggcaa tccacttggg
     2821 gctataaata attttgaaaa agccgagtat ggtatatatg caataaattc aaatctaacg
     2881 gtttataata atttattcaa ccacattcgt tcttctgatc aaatcaatga aggcaaagca
     2941 tcttgtattt atctcgaaaa ctcaaatggt tacggcaact tgaccgacat aaatcagtta
     3001 tggaagttat ataatataaa tgccgggaaa tctgacaatt ctcctaatta taataatgtt
     3061 ttcaaaaatt cccgaaatgg aatttacgga gtgaattgtg gtatcaactc atattacaac
     3121 caatttgata attgtaaaag agggataaaa gttatt
//
LOCUS       DAORQA010000210         3100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7439_length_3100_cov_11.553038, whole genome shotgun sequence.
ACCESSION   DAORQA010000210 DAORQA010000000
VERSION     DAORQA010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7439_length_3100_cov_11.553038"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PK736_08185"
     CDS             <1..1011
                     /locus_tag="PK736_08185"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRC33408.1"
                     /translation="VTHVAFGAAIGEILMGHKLGKKAMLIGAIACTVPDLDVFLTMGN
                     HDPLAYVQMHRSYSHAPFTHFFLALPMAYITYLLYKKKQDFLPLYGLWLVAMLLHSTV
                     DLCTTFGTQFLLPFTNQLVAFNSINIIDPLYTLPMLVWFFTAWVRKRSNFNSRKFAIV
                     AMVVSHLYMLSVLAVKGVAHRQFTNQLQQQHIPYTELSTTPAILNGMLWAGIAYNDSM
                     MSVGEYSIWDKDKNTRFVTFNRNRNLEEQYEGDKLNTVIWFSQGKYLMEAVTDTTARF
                     YIVKWGRFDFSKNTAEQTFKFYYTLTKSQGEVTITVHDPGDDDFKFSEAFAQLWQRIF
                     YN"
     gene            complement(1085..2365)
                     /gene="purD"
                     /locus_tag="PK736_08190"
     CDS             complement(1085..2365)
                     /gene="purD"
                     /locus_tag="PK736_08190"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009640678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRC33409.1"
                     /translation="MNVLLIGSGGREHALAWRISQSKLLTKLFIAPGNGGTRNHGENV
                     NIGINDFDGIANFCLQNNITMIVVGPEEPLVNGIWDYFHQNEKLRHISVIGPSKEGAQ
                     LEGSKHFAKEFMINNNIPTARFKTFDTLNIDSAKEFLQTLQAPFVLKADGLAAGKGVL
                     ILDSMEEALNELQLMIQQNKFGNAGTTVVIEEFLPGIELSMFVFTNGVDYIILPEAKD
                     YKRIGDNDTGLNTGGMGAVSPVPFANNEFKNKVEQQIIVPTIKGLQKEKINYCGFVFI
                     GLMNVKDNPYVIEYNVRMGDPETEVVMPRITSDLLAMFVAATNNTLKEYELTIDANYA
                     TTVMMVSQGYPEDYEKGKLITGNTTGEGIFHAGTAIKEGNLITNGGRVIAATGMAPTL
                     QAALQLSYEAVDSIQYENKYYRKDIGQDLMGFLK"
     gene            2539..>2845
                     /locus_tag="PK736_08195"
     CDS             2539..>2845
                     /locus_tag="PK736_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC33410.1"
                     /translation="MKRIFFLVLFCFCFINVQSQNKFTISGYVNEKGSKESLPGASIY
                     LPDLKSGTVTNNYGFYSITIPANDSLRIVISFIGYQPQIKILKLTQNISLNVELSSSI
                     "
     assembly_gap    2846..2855
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2856..>3100
                     /locus_tag="PK736_08200"
     CDS             <2856..>3100
                     /locus_tag="PK736_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HRC33411.1"
                     /translation="SEITVVEARSEKVSQSTQMSVIDIPIQQIKEIPALFGEKDVLKV
                     LQLMPGVQKGSEGSSGIYVRGGGSDQNLIILDDAIVY"
BASE COUNT          891 a          590 c          590 g         1019 t
ORIGIN      
        1 gtaacacacg ttgcatttgg tgctgccatt ggcgaaatat taatgggaca caagcttggt
       61 aaaaaagcga tgttgatagg tgctattgcc tgcacagtgc ccgatttgga tgtgttttta
      121 acaatgggca atcacgaccc gctggcatat gtacaaatgc atcgcagtta ttcgcatgct
      181 ccgtttacgc atttttttct ggcactgcca atggcataca tcacctattt gttgtacaaa
      241 aagaaacagg attttttgcc gctgtatggt ctttggctgg tggccatgtt gcttcattcc
      301 actgtcgact tgtgcactac tttcggaaca caatttttat tgccttttac caatcagctg
      361 gttgcgttta atagcatcaa cattatagac ccactttata ctttgccaat gcttgtatgg
      421 ttttttactg catgggttcg aaagcgaagt aatttcaact cgcggaaatt tgcaatagta
      481 gcaatggttg taagccattt atatatgtta agtgtattgg cagtaaaagg agtggcgcac
      541 cgtcaattta caaatcaatt gcaacagcaa catataccgt atacggagct gtctaccaca
      601 ccagcaattt tgaatggcat gctatgggca ggcattgctt ataacgatag catgatgagt
      661 gtgggcgaat attctatttg ggataaagac aaaaacaccc gttttgtaac tttcaacagg
      721 aatagaaatt tggaagagca gtatgagggt gataaattaa atactgtaat ttggtttagc
      781 cagggtaagt atttgatgga agcggtaacc gataccacag cgcgttttta tatagtaaag
      841 tggggtcgct tcgattttag taaaaacact gccgagcaaa cttttaagtt ttattatacg
      901 cttacaaaat cgcagggcga agtaaccatc actgtacacg acccgggcga tgatgatttt
      961 aaatttagcg aagccttcgc acaactgtgg caaaggattt tttataatta aaaacgaaat
     1021 atgttttaat tgtaacccgc aatgttttgt ttgcagattc gattaagcta ataattgcga
     1081 gaagttattt caaaaaaccc atcaaatcct gccctatatc tttcctgtaa tatttgtttt
     1141 cgtattgaat tgaatcaact gcttcatagc ttaattgtaa agctgcttgc aatgttgggg
     1201 ccatgcctgt tgcagctatc acacgtccgc cattggttat caaatttcct tctttaattg
     1261 cagtaccggc atgaaaaatt ccttcgccag ttgtattgcc ggtaatcaat ttcccttttt
     1321 cataatcctc ggggtagcct tgcgaaacca tcatcactgt agttgcataa ttggcatcga
     1381 tggtaagctc atattctttc agtgtgttat ttgttgcagc cacaaacatg gcaagtaaat
     1441 cgcttgttat gcgcggcatc actacttcgg tttcggggtc gcccatgcgc acattatatt
     1501 ctatcacata tggattatct tttacattca tcaatccaat aaaaacaaaa ccgcagtaat
     1561 ttattttttc tttttgcaac cctttaatag ttgggacaat aatttgttgc tctactttgt
     1621 ttttaaattc attgttagcg aatggcacag gcgatactgc acccatgcct cctgtgttta
     1681 agcctgtatc gttatcacca attcgtttat aatcttttgc ttcgggcaat atgatataat
     1741 ccacaccatt agtaaaaaca aacatcgata gttcgatgcc gggtaaaaat tcttcaatca
     1801 caactgtggt tccggcattg ccaaatttat tttgttgtat catcagttgc aattcgttca
     1861 gtgcctcttc catcgaatct aaaatcaaca caccttttcc agctgcaagt ccatcagctt
     1921 ttaaaacaaa tggtgcttgt aatgtttgca aaaattcttt tgcgctgtca atatttagtg
     1981 tatcgaaagt tttgaaacgg gcagtaggaa tattgttgtt gatcataaac tcttttgcaa
     2041 aatgtttgct gccttcaagt tgtgcccctt cttttgatgg ccctattact gatatgtggc
     2101 ggagtttttc attttggtga aaataatccc aaatcccatt tacaagaggc tcttcgggac
     2161 ctaccacaat cattgtaata ttattttgca ggcagaaatt tgcaatgcca tcaaaatcgt
     2221 taatgccaat atttacattt tcgccatgat tgcgcgtgcc accattacct ggagcaataa
     2281 ataattttgt gagtaattta ctttgcgaaa tgcgccatgc caatgcatgt tcgcggccac
     2341 cacttcctat aagtaataca ttcattgtga aattattttt tcgattgttt gcgaatgcaa
     2401 atcaccacaa aactttataa tgcgcaactt tttttatttg ttctgagaaa ttattttttc
     2461 cacttacgca tgtctaagca atttttacca tggtatttcg ttacgaaatt ttacttttgt
     2521 aatcattaca tggaatcaat gaaaagaata ttttttctcg ttttgttttg cttctgcttt
     2581 ataaatgtac aatcgcaaaa taaatttacc atcagtggat atgtaaatga aaagggcagc
     2641 aaagagtcgt tgcccggggc aagtatatat ttgcccgatt taaaatcggg aacagttacc
     2701 aacaactatg gattttatag tattacaatt cctgccaacg attcgctacg catagtaatt
     2761 tcctttattg gatatcagcc gcagataaaa atattaaagc ttacacaaaa tatttcgctc
     2821 aacgtagagc taagttcgag tatcgnnnnn nnnnnttagt gaaattacag ttgtagaagc
     2881 acgatccgaa aaagtgagcc aaagcacaca aatgagtgtg attgatattc ccatccaaca
     2941 gataaaagaa attcctgctt tgtttggcga gaaggatgtg cttaaagttt tgcaactaat
     3001 gcccggtgtg caaaaaggta gtgaaggcag ttcgggtatt tatgtgcgcg gtggtggcag
     3061 cgatcaaaat cttataattc ttgacgatgc cattgtgtat
//
LOCUS       DAORQA010000211         3077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7499_length_3077_cov_12.800132, whole genome shotgun sequence.
ACCESSION   DAORQA010000211 DAORQA010000000
VERSION     DAORQA010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7499_length_3077_cov_12.800132"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..665)
                     /locus_tag="PK736_08205"
     CDS             complement(75..665)
                     /locus_tag="PK736_08205"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HRC33412.1"
                     /translation="MAKPISKREITRLPKANYEDIRSSLKTGDLVFCSGNYYFSKVIQ
                     YFTKSVWSHVGVIYKDEQLGRVLILESEVGIGVRLAPVSKYLKDYHGRNKPYKGNIVV
                     ANINPHVDVKQVNNAISFGLDELTKPYDNFEILRIMIRILFRITRRTRDRKYICSELI
                     DACYHKAGLKFSIFDNYISPYDIWRDSKVEMVARIL"
     gene            complement(743..>3077)
                     /locus_tag="PK736_08210"
     CDS             complement(743..>3077)
                     /locus_tag="PK736_08210"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRC33413.1"
                     /translation="FQGEQVDGHDNDTWLGGAKEINDFREANGTEPLWTNRMFSGMPA
                     YQISMESKNNLLLYVYKGLLKICPFPAFFIFAGLISFYVLLITLKFDYRLAIAGGISF
                     AMCSFNFYIIQAGHNSQALAIALMPLVVAGILQTFRGNYLLGAGLTAFGMAFEIMANH
                     VQISYYLGLMLMVFFISELYFATKNKTMVKYFTACAIIGVASLIGVMSNSTSLLTTYE
                     YGKDSTRGKSELTKVNAPASSGLDIDYAFQWCYGKAETFNLLVPNILGGPSQSDIGTN
                     SKTYDYIAKNFGADQALSFSQKAPAYWGDQPSVNGADYMGAMAIFFFAVGMFLVQGRK
                     KWWLFAATILSFVLSWGKNFMAFNEFWYNNFPLYNKFRSVSFILIIASFTIPFLSMLT
                     LKKLFDAKVALAEKQKALMRGLYVVGGLLVLILIGSAGFSYSGPVDTNFAQNPDLLVA
                     LKADRAKLLRMDTLRSLFFVLLAFGAIWFYLKKKLDMKYVLIALPILFLIDMWPISKR
                     YLNDSHFKPKQQTDNPFTKSAADEQILQDKELGYRVINTTVSTFNDASTSYYHNSIGG
                     YHGAKLERYQELIENHISKNNMSVLNMLNTKYFIVSPKEGQSPMAQMNPEHCGSAWFV
                     PEFKIVANADEESKSLEKFNPRVTAFIDQRFKDKIGNFKINYDSVATIQLTKYDANYL
                     VYKSKCATDQLCVMSEIYYEKGWNAYVDGKLTPHWRCNYVLRSMIVPAGNHTIEFKFE
                     PVIYKTGETISLASSSIMLLLFFGALIIPFFRKQKSA"
BASE COUNT          991 a          628 c          526 g          932 t
ORIGIN      
        1 gcattatata aatatttttt gtcaattcca ccaactattt ttgagctcat taccacaaat
       61 ccaattctta acccttacag tatacgtgcc accatttcta ctttactatc gcgccaaata
      121 tcgtaaggac ttatataatt atcgaatatg gaaaatttca aacctgcttt atggtaacaa
      181 gcatcaatta gttcgctgca aatatatttg cggtcgcggg ttctacgtgt aatgcgaaaa
      241 agtatgcgaa tcataatgcg caaaatttcg aagttatcat aaggctttgt aagttcatcg
      301 agcccaaacg aaatggcatt atttacctgc ttaacatcaa catgtggatt aatgtttgca
      361 acaacgatat tgcctttgta tggtttgttg cgaccgtggt agtctttcag gtatttcgaa
      421 accggagcaa gtctcactcc tattcccact tcgctttcga gtataagaac gcggccaagt
      481 tgctcgtctt tatatatcac tcccacatga ctccaaaccg acttagtaaa atattgaatg
      541 actttcgaaa aataataatt gcccgaacaa aaaaccagat cgccagtttt caagctacta
      601 cgtatatcct catagttagc ctttggcaag cgggtaattt ctcttttact tattggctta
      661 gccattactt tacaggatat tatttattcc gaataccaag gatttccatc ataaaaacaa
      721 aacaataaaa tcacttgcaa ttttatgcag atttctgttt tctgaaaaat ggaattatta
      781 aagcaccaaa aaataacaac aacattatgg atgacgaagc aagcgaaatg gtttcgcctg
      841 ttttataaat cacaggttcg aatttaaatt ctatagtatg attccctgca ggaacaatca
      901 tactacgcaa aacataattg cagcgccagt gtggcgtaag ctttccatca acataagcat
      961 tccaaccttt ttcgtaatat atttcactca tcacacaaag ttgatctgta gcacatttcg
     1021 atttatatac aagatagttg gcatcgtatt ttgttaactg aatggtagct acactatcgt
     1081 agttaatttt aaagtttcca attttatctt taaacctttg gtcgataaaa gcggtaacac
     1141 gagggttaaa cttttcgagc gatttcgact cttcatcggc atttgcaact attttaaatt
     1201 cgggcacaaa ccaagctgaa ccgcaatgtt ccggattcat ttgtgccatt ggagattgtc
     1261 cttcttttgg tgacacgata aaatattttg tattgagcat attaagcaca ctcatgttgt
     1321 ttttcgaaat gtgattttct atcaattcct gataccgctc gagttttgct ccatgatatc
     1381 caccaataga attatgatag taggaagtgc ttgcatcgtt gaaggtgctt acagtggtgt
     1441 tgattacacg ataacctaat tctttatctt gaagaatctg ctcatcggct gcgctttttg
     1501 taaatggatt atcggtttgt tgctttggtt taaagtgtga gtcgtttaag tatcttttac
     1561 ttataggcca catatctatt aaaaacaaaa tgggtaatgc aatcaaaacg tatttcatat
     1621 cgagtttttt cttcaggtaa aaccaaatgg caccaaaagc aagaagaaca aaaaacaaac
     1681 tgcgcaatgt gtccatgcga agcaatttag cacgatcggc tttcaatgca acaagcaaat
     1741 cggggttctg cgcaaagttt gtatcaacag gtcctgagta actaaacccg gcagagccaa
     1801 ttaatatcaa aaccaaaaga cctccaacaa catataagcc acgcatcaaa gccttttgtt
     1861 tttcggcaag tgctactttg gcatcgaaca attttttcaa tgttagcatt gataaaaatg
     1921 gaatggtgaa tgaagcaatg ataagtatga atgataccga acgaaactta ttatacaatg
     1981 gaaaattatt gtaccaaaac tcgttgaagg ccatgaaatt ttttccccac gataaaacaa
     2041 aactcaggat ggtagctgca aagagccacc atttttttcg gccctgcact aaaaacattc
     2101 ctacagcaaa aaagaaaatg gccattgcac ccatataatc tgccccattt acacttggtt
     2161 gatcgcccca gtatgcaggc gccttttgcg aaaatgataa tgcctgatcg gcaccaaaat
     2221 ttttagctat ataatcatat gttttcgagt tggtacctat atcactttgc gaagggccgc
     2281 ctaaaatatt tggaaccaat aaattgaatg tttcagcttt accataacac cattgaaatg
     2341 cataatcaat atcaagtccc gatgatgccg gggcgtttac ttttgtcaac tcactcttac
     2401 cacgtgtgct atctttacca tattcgtagg tagtgagcaa cgatgtactg ttcgacatta
     2461 caccaataag cgaggccaca ccaataattg cacatgctgt aaaatacttt accatggttt
     2521 tgttcttagt ggcaaaatat aattcggaga taaaaaacac cataagcatc aatccaaggt
     2581 aataagatat ttgaacatgg ttagccataa tttcaaatgc cataccaaat gcggtgagtc
     2641 cggcacccaa caaataattg ccacgaaatg tttgcaatat acctgctacc acaagtggca
     2701 tcaatgcaat ggctaatgct tgcgaattgt gccccgcctg tatgatataa aaattgaaag
     2761 agcacatggc aaaacttatt ccaccggcta ttgcaagtcg ataatcgaac ttgagggtga
     2821 tgagtaatac ataaaaactt ataagcccgg caaagatgaa aaatgccgga aagggacaaa
     2881 tttttaataa acctttataa acatacaata acaaattatt tttcgactcc attgaaattt
     2941 gataagccgg cataccgcta aacatgcggt ttgtccaaag cggttcggtg ccattggcct
     3001 cacgaaaatc attaatttct tttgctccac ccaaccatgt atcgttatcg tgcccatcta
     3061 cttgttcccc ctgaaac
//
LOCUS       DAORQA010000212         3070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7519_length_3070_cov_5.667993, whole genome shotgun sequence.
ACCESSION   DAORQA010000212 DAORQA010000000
VERSION     DAORQA010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7519_length_3070_cov_5.667993"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PK736_08215"
     CDS             <1..425
                     /locus_tag="PK736_08215"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC33414.1"
                     /translation="KAMDDLLLYCQKAFVQADIEADIKEGKAFYAICKLDERDCGYIK
                     LRSDRTLDSLPKLRCIELERIYVGRDYWRHHIGKFLMDEAVSFGRKNNFEYLWLGVWQ
                     LNHRANAFYKNYGFEIIGTKKFYVGTEENDDYVLGIRI"
     gene            834..2189
                     /locus_tag="PK736_08220"
     CDS             834..2189
                     /locus_tag="PK736_08220"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRC33415.1"
                     /translation="MRIFISYSHCEDFKGHMKRVFEYLNTVFQKEEVNGFYYDKDNFW
                     LDENEIQAGNNYDKLIKDGINNSDYFILFFSNPYLQSTYVSETELPEIIKLCKERGTL
                     NNIIPVRFAKTIDKFWTTAEHKYFLEEKISFAIGNKTMYSTAMEFENNLTDFVNEIKD
                     RLFPGVLKPNTIPPPVLTANSEKIKNNRFRINRENQFNNFKDSILFRLKILKKPAADK
                     LKSGILFFSGSFDQLINNFVLRIPRSLGDYLKNENYNFSKLDRTQNLKNHLKGEARIV
                     DEGLSADIAIDMIVDRMSATAQKFLKDESIKAGEKIIIPLFLWFKDDVSYVIALFDRL
                     KTDLHQKVCEGDHDIFTKFESICYFISVIYDDDLKGSSFEKNLLSAINSAANKDCVWH
                     ISGFDDVSKDDFSNWLDLLSEDSIDTASIKNNYPSLFANAQTISMNKIDDYIFNEKYL
                     S"
     gene            2202..>3070
                     /locus_tag="PK736_08225"
     CDS             2202..>3070
                     /locus_tag="PK736_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRC33416.1"
                     /translation="MASPFTTSSTTQILREKLSGFVNKPTDDNPYVLTQELADAIDVA
                     LLLGQPLLITGEPGTGKTVLAQCLQKLFDLELYVFNTKTTSLANDLLYQYDMLRHFVD
                     AQIQKDKLTDIENYISMGMLGRAFEKALINKQNAMSADTASKLKMRSIVLIDEIDKAP
                     RDFPNDLLNELDRYRFTVKETQKEVACDPDLKPIIIVTSNTEKNLPEPFLRRCVFHHI
                     SFEKDRYKMLDDIVRAWFGAGNDGLYQKAKAMFIDLRNNNNMQKKPATAEFLGWFHLL
                     IEMSKQNKEINEG"
BASE COUNT         1066 a          508 c          577 g          919 t
ORIGIN      
        1 aaaaggccat ggacgacctt cttttgtatt gccaaaaggc atttgtacaa gccgatattg
       61 aagccgatat aaaagaaggc aaagcatttt acgccatatg caaactagat gaaagagatt
      121 gcggatatat aaagctccgc agcgatagaa ccctcgatag tttgccaaaa ctccgatgca
      181 tagaacttga acgcatatat gtaggccgcg attattggcg acaccacatt ggcaaattcc
      241 tgatggatga agctgtttcg tttggccgca aaaataattt cgaataccta tggttgggcg
      301 tttggcaatt gaaccatcgt gcaaatgcct tctacaaaaa ttatggcttt gaaataattg
      361 ggactaaaaa attttatgtt gggactgaag agaatgacga ttatgttttg ggaataagga
      421 tttaagattt tattttaatg caagggtaaa cccccttgac atgagtaatg ctgttgtgtt
      481 actttgaatt tatatggatc gtaaaaatgg taattacaaa ttgaagaatt tgtaatgtta
      541 cgattgagag aatatagtgt atttcaagtt ttatgtggcg ggtttttaaa tttattttga
      601 aatggaaatt tctgctgaac aaggatggaa aaatattttg tttcaagtga aaataatatc
      661 ctatttttac aaatgccgat aatgaacata cctattcgtt tttagggcac atgttgtaga
      721 aaccaaaaca tagagacctt gacttttttt agaaatgatg ctttgaaaag tgcgtacaac
      781 aaaatgatag cgataatgca aaagaaaatt caacagaagt aatatccggg cttatgcgta
      841 tttttatttc ttattctcac tgcgaagatt ttaaaggcca tatgaaaagg gtgttcgaat
      901 atttgaatac cgtttttcag aaggaagaag tcaatggatt ttattacgat aaggataatt
      961 tttggctcga tgaaaacgaa attcaagctg gtaacaacta cgataaactg attaaagatg
     1021 gaataaataa cagcgattat ttcatattgt ttttttctaa tccctatctg caatcgacct
     1081 atgtgagtga aactgaatta cccgaaatta taaaactttg taaggaaaga ggtactctca
     1141 acaatattat tcctgtgagg tttgcaaaaa ccatcgataa attttggacc actgccgagc
     1201 ataaatattt tttagaagag aaaatttctt ttgcaattgg taacaaaaca atgtattcga
     1261 ccgcaatgga attcgaaaat aatttaaccg attttgtaaa tgaaataaaa gaccgtttat
     1321 ttccgggtgt actcaagcca aacaccatac cgcctcccgt tcttaccgca aatagtgaaa
     1381 aaataaaaaa taacagattt cgaattaaca gagaaaatca gtttaataat tttaaagaca
     1441 gcatactctt tcgcttaaaa atactgaaaa agcctgctgc cgataaatta aaaagtggga
     1501 tactcttttt ttcgggttca ttcgaccaat tgatcaataa ttttgtttta cgcattcctc
     1561 gaagtttggg tgactattta aaaaatgaga actacaactt ttcgaaactt gaccgcaccc
     1621 aaaatttaaa aaaccatctc aaaggcgaag cacggatagt tgacgagggc ttgtctgccg
     1681 atattgccat agatatgata gtagacagaa tgagcgcaac ggctcaaaaa tttctgaaag
     1741 acgaaagtat aaaagccgga gaaaaaatta tcattccact gttcctttgg tttaaggatg
     1801 atgtaagtta tgtcattgca cttttcgata gattgaaaac cgacctgcac caaaaagtat
     1861 gcgaaggcga ccatgatatt tttacaaaat tcgaaagcat ttgctatttc atttcggtta
     1921 tttacgatga cgatttaaaa ggaagctcat tcgaaaaaaa cctgctaagc gctattaatt
     1981 ctgctgccaa taaggattgt gtttggcata tttcgggttt cgatgatgtg agtaaagatg
     2041 attttagtaa ctggctcgat ttgttgagtg aagatagtat cgatactgca tccattaaaa
     2101 acaattatcc atctttattt gctaatgctc aaactataag catgaataag attgacgatt
     2161 acattttcaa cgaaaaatat ttatcataaa aataaaatac aatggcatca ccattcacta
     2221 catcatccac cacgcaaata ctgcgcgaaa aactttcggg gtttgtaaat aaacctacag
     2281 atgataaccc ttatgtgctt actcaggaac tggcagatgc catagatgtt gctttgttac
     2341 tggggcaacc cctgttaata acaggcgagc ccggcactgg caaaactgtt ttggcgcaat
     2401 gccttcaaaa acttttcgat cttgaactat atgtttttaa tacgaaaaca acttcgcttg
     2461 ccaacgactt gctgtatcag tatgatatgt tgcggcattt tgttgatgcc caaattcaaa
     2521 aagacaaatt aacggatatt gaaaattaca tttcgatggg tatgcttggc agggcatttg
     2581 aaaaagcgct tatcaataaa caaaatgcaa tgtctgccga cacagcatcg aaattaaaaa
     2641 tgcgaagtat agtgcttata gatgaaattg ataaagcacc gcgcgatttc cccaacgatt
     2701 tattgaatga attagaccgg taccgtttta ccgttaaaga aacacaaaag gaagttgcat
     2761 gcgatcccga tttaaagccc ataataattg taacaagtaa taccgaaaaa aatcttcccg
     2821 aacctttttt gaggcgttgt gttttccatc acatttcttt cgaaaaagac agatacaaaa
     2881 tgcttgatga cattgttcgt gcctggtttg gcgctggtaa cgatggacta taccaaaaag
     2941 caaaagctat gtttatagat ttgcgcaata acaataacat gcaaaaaaaa cctgccactg
     3001 ctgagtttct tggttggttt catttgctga ttgaaatgag taaacagaac aaagaaatta
     3061 atgagggaaa
//
LOCUS       DAORQA010000213         3032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7612_length_3032_cov_6.505878, whole genome shotgun sequence.
ACCESSION   DAORQA010000213 DAORQA010000000
VERSION     DAORQA010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7612_length_3032_cov_6.505878"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PK736_08230"
     CDS             <1..770
                     /locus_tag="PK736_08230"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRC33417.1"
                     /translation="IYNQQNRQTMFVVTGCMPFEFTEYKWTFPMIGEVTYKGFFDSVA
                     ANNEMMELQKQGYETNIGNASGWSTLGWLRDPVLSTMLNKSEGALAELIIHELTHATI
                     YQSSSVVYNENLATFVGENGALKFLIAHYGDTNVYIDRYRNRLSDELLYNNHIQNGRL
                     QLQSLYLKLKNEKDVTLKTKKKTDLINNIMTSIDTITLKNKLRYTWDYKIDSLPNNTF
                     FMSTDTYHSMQNIFMAEFKNKYANDMRKFIEGHSGEK"
     gene            complement(799..1194)
                     /locus_tag="PK736_08235"
     CDS             complement(799..1194)
                     /locus_tag="PK736_08235"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRC33418.1"
                     /translation="MKKIKRGFFIIFCAVVVFALYNYKMTFYLLSQLKGQLTIVYKAE
                     SIDEAIASKKYDSLQTQKLILIKAIKQFAEDSLGLSKTKNYSTIYNQQNRLVHAYDSL
                     DNSIVWAILNNHLKALEDEIRGLSQFGDL"
     gene            complement(1275..>1564)
                     /locus_tag="PK736_08240"
     CDS             complement(1275..>1564)
                     /locus_tag="PK736_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33419.1"
                     /translation="LLTYPLQLFVIKNPIGLLKNIFKVLFKNTNWVGPDYNNNSNERV
                     HKSILHPSHQIGLQKLDNATIDRLNMLYAKNFNIEYDINMVMKNYRMLGSV"
     assembly_gap    1565..1623
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1624..3024)
                     /locus_tag="PK736_08245"
     CDS             complement(<1624..3024)
                     /locus_tag="PK736_08245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC33420.1"
                     /translation="MSKGEYVLLLNPDTLVEEDTFTSTVNFMDAHSDAGGLGVYMIDG
                     KGNFLPESKRGLPSPSVAFYKIFGLSALFPKSKLFGRYHLGFLDKNQIHEVDVLSGAF
                     MLMRKSVLDKVGLLDETFFMYGEDIDLSYRITQGGYKNYYFPETKIIHYKGESTKKTS
                     VNYVRVFYKAMVIFARKHFNKQHAWLFGSLINFAVYLRALMAVAVQFLKRIAIPLLDS
                     LIMYGGYIALKYYWELKIKVFHYPSFYETVIIPVYVLCWLIGIYLAGGYDKPVRYIKV
                     IRGITFATIAILVVYALMPEEYRFSRALILMGTLWSAGALVVYRWICQVFKIPLFSFA
                     GNIKKRVVIVGGETECHRILLLLQNSNSNFEYVGFTDIAKSELPQSNQNPNYLGDYKR
                     LQDICEVYKIDEVIFSSKDIPSQNIISTMRENAASAIEYKIAPEESSYIIGSNSIHSQ
                     GSLYFVEMNTLNRIENI"
BASE COUNT          959 a          567 c          521 g          926 t
ORIGIN      
        1 ccatttacaa tcagcaaaac aggcaaacca tgtttgtagt tacaggctgt atgccctttg
       61 agtttaccga atacaagtgg acatttccca tgattggcga agtaacctac aaaggttttt
      121 ttgatagtgt ggctgctaac aacgaaatga tggaattgca aaagcaaggt tatgaaacca
      181 atattggcaa tgcatcgggc tggagtacgc ttggttggct gcgcgaccct gtgctgagta
      241 caatgctcaa caaaagcgaa ggagcacttg ccgaattgat aattcatgag ttgacacatg
      301 ccacaattta ccagtcgagc agtgtggtgt acaacgagaa cctcgcaact tttgttggag
      361 agaatggcgc cttaaaattt ttgattgctc actatggtga taccaacgta tacatcgacc
      421 gttacagaaa tcgattaagc gatgaattgc tttataacaa tcacatacaa aatgggcggc
      481 tgcaattgca aagtctttat ttaaaattga agaatgaaaa agatgtcact ctcaaaacaa
      541 aaaagaaaac tgatttgatt aataatatca tgacatcaat tgatactatt acattgaaaa
      601 acaagttacg gtatacatgg gattacaaaa tagattcgtt acccaataac acttttttta
      661 tgagtacgga tacttaccac agcatgcaaa atatttttat ggctgagttt aagaataagt
      721 atgcgaatga tatgaggaag tttattgagg ggcattctgg tgaaaaatga tttattctct
      781 tcacaacgca cattctttct ataaatcacc aaattgactt aatcctctta tttcgtcttc
      841 taaagctttt agatgattat ttaatatagc ccaaacaata gagttgtcca aactgtcgta
      901 agcatgaacc aacctgtttt gctgattgta aatggtggaa taatttttgg ttttcgataa
      961 gcctaacgag tcttcggcaa attgttttat tgcctttatt aaaataagtt tttgggtttg
     1021 aaggctatcg tattttttgc tggcaatggc ctcatcaata ctttctgctt tgtaaacaat
     1081 agttagttgt cccttcaatt gtgagagcaa ataaaaggtc attttgtaat tgtacaatgc
     1141 aaacacaaca acggcacaaa atattatgaa gaaaccacgc tttatttttt tcatttaacg
     1201 taaacacata tttacttaac ggctcaagtt taatatggtg gttaaaaagt attttaaatc
     1261 agtaaatctc atgtttaaac actgcccaac atacgatagt ttttcattac catattgata
     1321 tcgtactcta tgttgaaatt ttttgcatac aacatgttca atctatctat ggttgcatta
     1381 tccagttttt gcaagcctat ttggtgcgaa ggatgcaaaa tgcttttatg taccctttcg
     1441 ttgctgttgt tattataatc gggcccaacc cagttggtat ttttaaataa aactttgaat
     1501 atgtttttca ataagccaat aggatttttt atcacgaaaa gctgaagagg ataggttagg
     1561 agcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnaatattt tctattctgt tcaaggtgtt catctctaca aaatacaaac tgccctgtga
     1681 atgaatagag ttggaaccaa tgatatacga actttcttca ggcgcaattt tgtattcgat
     1741 ggccgatgca gcattttcgc gcatggtact tataatgttt tgcgatggta tatccttcga
     1801 tgaaaatata acctcatcta ttttgtacac ttcgcatata tcctgaaggc gtttatagtc
     1861 gccaaggtaa ttggggtttt gattgctttg tggcaattcg cttttagcaa tatctgtaaa
     1921 accaacatat tcgaaattcg aattactgtt ttgtaacaat aacagtatac gatggcactc
     1981 tgtttcacca ccaactatta ccacacgttt tttaatgttg ccggcaaacg agaacaatgg
     2041 aatcttaaaa acctggcata tccatctgta cacaaccaat gcacctgccg accacagtgt
     2101 acccatcaaa atcaaagcgc gcgaaaagcg gtattcttct ggcatcaaag catacaccac
     2161 caatatggca atagttgcaa aggtaatacc gcgaatcact tttatatatc gtacaggttt
     2221 gtcgtaacca ccggccaaat aaattccaat taaccaacat agcacgtaca caggaataat
     2281 taccgtttcg taaaacgatg gataatggaa aacttttatc ttcaattccc agtagtactt
     2341 taatgcaatg tatcctccat acattatcaa tgaatcgagc agaggaatgg caattcgttt
     2401 tagaaattga actgcaactg ccatcaaagc acgaaggtaa accgcaaaat ttatgagcga
     2461 tccaaatagc catgcatgtt gcttgttgaa atgtttacgg gcaaaaatga ccatggcttt
     2521 gtaaaaaaca cgcacataat tcacgctcgt cttctttgta ctctcgcctt tgtaatgaat
     2581 aatttttgtt tcgggaaaat agtaattttt atatcctccc tgtgttatgc ggtacgataa
     2641 atcgatatcc tcaccataca taaaaaaagt ttcatcgagc aaaccaactt tatcgagcac
     2701 acttttacgc atgagcataa atgcacccga taatacatcc acttcgtgaa tttggttttt
     2761 atcaagaaac cccaaatgat accgtccaaa taatttagac tttggaaaca aggccgacaa
     2821 gccaaatatt ttgtaaaaag ctacagaagg acttggcaaa ccacgtttcg actccggcaa
     2881 aaagtttcct ttaccatcta tcatgtatac acccaggcct ccggcatcgg aatgtgcatc
     2941 cataaaatta acggtgcttg taaacgtatc ttcttctacc aatgtatcgg gattaagcaa
     3001 tagtacatac tcgcctttgc tcaattttat gc
//
LOCUS       DAORQA010000214         2993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7743_length_2993_cov_7.074881, whole genome shotgun sequence.
ACCESSION   DAORQA010000214 DAORQA010000000
VERSION     DAORQA010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7743_length_2993_cov_7.074881"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1078
                     /locus_tag="PK736_08250"
     CDS             <1..>1078
                     /locus_tag="PK736_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33421.1"
                     /translation="AHSFTDFPFALARYNTNGSLDSTFDTDGKVTTNFGLACQANSMA
                     IQADNKILVAGSSNNGNKNDFALVRYNTNGSLDTSFDDDGIVTTQIGLYDDVGSSVAL
                     QTDGKILVSGYTYDSVYCNFALVRYNINGSLDTSFGTDGIVLTTISSYNDFCNSLTIQ
                     GDGKIILAGSSDNGNAYDIALARYNTNGSLDTTFEGDGIVTTSIDTTSAFGSAIGIQS
                     DGKIVVSGGHHNGTYSDFALVRYNNNGSIDSTFDSDGIVVTDFGNFAQIKSLVLQTDD
                     KIVVAGYSYVSPNFYFAIARYNINGSLDTSFDSDGIVTTDFGTINNVARAVAIQSDGK
                     ILAGGNTNPLIFAIARYNVCIPVLS"
     assembly_gap    1079..1113
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1114..1859
                     /locus_tag="PK736_08255"
     CDS             <1114..1859
                     /locus_tag="PK736_08255"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33422.1"
                     /translation="TVGINNYTVSGIFIDTLISYSNGCDSIVTTNLNVYPLTTFTQSF
                     TICVGQTITIGNNTYSVSGTYIDTLSQISNCDSIVTTNIMVTPMPLITTTVNGIIIIA
                     NQTAATYQWIDCINNNMPIANATNQTYTPIVNGSYAVAVSFGNCVDTSACVMISTVGV
                     SPMPNKEAQISILPNPFTTTTTLQTNINLNDATVIFYNLFAQAVKKVNNITGNSISIK
                     RDNLPSGFYFVQLIQDNKIIVTSKIVIAD"
     gene            complement(2191..2868)
                     /locus_tag="PK736_08260"
     CDS             complement(2191..2868)
                     /locus_tag="PK736_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33423.1"
                     /translation="MKKNTSVYTYSYVALILLIFVANVMMAQRIQSGKIIYTAAYIKA
                     NEKDKLPGGLPTQCVLLFNDKLYRTETRYENDSSIAVMITDEAAGLITQLLNFPGNKL
                     AVQSKIKKYYKGQSFKLQMMTDTKKIAGYECTKIIITQDDSLITEAWYTKSIVRTGKP
                     NKAFHNVDGAFLEYSVALDKNGTMMKYTATEVVEQKIADDVFQVPSTYKVCTQKKLNR
                     MLGARFF"
BASE COUNT          934 a          494 c          528 g         1002 t
ORIGIN      
        1 gcacatagtt tcactgattt tccttttgct ttagcacgtt acaacacaaa tggcagcttg
       61 gattccacat ttgatactga tgggaaagtg acgacaaatt ttggcttagc ttgtcaggca
      121 aactcaatgg ccatacaagc ggataataaa attttggtgg cgggttcaag taacaacggg
      181 aataaaaatg attttgcttt agtacgctac aacacaaatg gaagtttaga tacttccttt
      241 gatgatgatg gaatagtcac aactcaaatt ggactttatg atgatgtagg ttcttctgtt
      301 gctttacaaa ctgatggcaa aattttggtg tctggatata catacgatag tgtgtattgt
      361 aattttgctt tagtgcgtta caatataaat ggtagtttag acacttcgtt tggcacagat
      421 ggtattgtac ttaccactat aagctcctac aacgattttt gcaattcgct gacaattcaa
      481 ggtgatggta aaattatatt agctggaagt agcgataatg gaaatgcgta tgatattgct
      541 ttagctcggt acaacactaa cggtagctta gacacgacat ttgaaggtga tggaattgtg
      601 acaacctcaa ttgacacaac ttcggctttt ggaagtgcta ttggaattca aagcgatggt
      661 aaaatagtgg tgtcaggagg acaccataat ggcacttata gtgattttgc tttagttcga
      721 tacaataaca atggtagcat agacagcact tttgacagcg atggaattgt tgttactgat
      781 tttggaaatt ttgcgcaaat caaatcctta gttttgcaaa ctgatgataa aattgtagtt
      841 gctggttata gttatgttag tcctaacttc tattttgcaa tagcacgtta taacattaat
      901 ggcagcttag ataccagctt cgactcagat ggtattgtta ctactgactt tggaacaata
      961 aataatgtag ctagagcagt tgcaatacaa agcgatggta aaattcttgc tggaggaaac
     1021 actaacccat tgatttttgc tatcgcacgg tataatgttt gtattccagt tttatcatnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntaactgt tggaattaat aactatacag
     1141 taagtggcat ttttatagat acattaattt cttactcaaa tggatgtgat agtattgtaa
     1201 ctactaattt gaatgtatat cctttaacca catttactca atcatttaca atttgcgttg
     1261 gccaaacaat aaccataggt aacaatacat attcagttag tggaacctat attgatacac
     1321 tctcacaaat tagtaattgt gatagtattg tcacaacaaa cataatggta actccaatgc
     1381 ctcttataac cacaactgta aatggaatta taattatagc aaaccaaacc gcagctactt
     1441 atcaatggat tgattgtatc aataataata tgccaattgc caatgcaact aaccaaacgt
     1501 atacacctat agttaatgga agttatgctg ttgccgtttc gtttggcaat tgtgttgata
     1561 ccagtgcatg tgtaatgatt tcaactgttg gcgtttctcc aatgccaaat aaagaagcgc
     1621 aaatttcaat actacccaat cctttcacca ccaccaccac tttgcaaacc aacattaatt
     1681 taaatgatgc aacagtaatc ttttataatt tatttgcaca agcagtaaaa aaagtaaata
     1741 atattacggg taactccatc tcaattaaac gcgacaattt accaagcgga ttttattttg
     1801 tacaactgat acaagacaat aaaattattg ttactagtaa aattgtaatt gcggattgaa
     1861 ttggttagtt ggcaggtgat tgcaacaagt aatctcgaat tgttttgaat ttcccttttt
     1921 ccacccagtc acagaggctt caaaatgtaa gatatttaac aactcgtaaa aaatttatgg
     1981 gaaataattc gtaaacttgt gcaatagttt tgcttcctta actccaacga aaatccatgt
     2041 gtgcaaggga gaaatttgca aatgccattg agtataaaaa gattttgaga aataaggagt
     2101 gaagcttttc aaacaatact tgttctgttt tttcttgcat gtaaaaacac aaattccaaa
     2161 caaaacgaat caggggcaaa atattcactt ttaaaaaaac cgtgcgccaa gcattctatt
     2221 caatttcttt tgggtgcata ctttataagt tgatggcact tgaaaaacat cgtcagctat
     2281 tttttgttca acaacttctg tggctgtgta tttcatcatt gttccgtttt tatcgagtgc
     2341 aacagaatac tctaaaaaag caccgtccac attatgaaac gctttattgg gtttgcccgt
     2401 acgcacaata gatttagtgt accacgcctc ggtaattaac gaatcgtcct gagtaatgat
     2461 tatttttgtg cattcgtaac cggcaatttt ttttgtgtcg gtcatcattt gcaatttgaa
     2521 agattggcct ttgtaatact tttttatttt cgattgcaca gcaagtttat ttcccggaaa
     2581 gtttagcaat tgcgttatca atcctgcagc ttcatcggta atcatcacag caattgacga
     2641 atcgttttcg taccttgttt cggtgcggta tagtttatca ttaaacaaaa gcacgcattg
     2701 tgttggcaaa ccacctggca gtttatcctt ttcatttgcc tttatataag cggctgtata
     2761 aataattttt cccgattgaa ttcgttgtgc catcattaca ttggcaacaa aaataagaag
     2821 gataagggca acataactat atgtgtatac gcttgtattt tttttcatct ggttattttg
     2881 ttattcagtt cttgtgttat tttgttactg gttattgcgt tattcagtta ttggttattt
     2941 tgttattacg ttattaagtt atagaacaat tcaaagagag aacgagatta aaa
//
LOCUS       DAORQA010000215         2965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7834_length_2965_cov_6.426117, whole genome shotgun sequence.
ACCESSION   DAORQA010000215 DAORQA010000000
VERSION     DAORQA010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7834_length_2965_cov_6.426117"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..311
                     /locus_tag="PK736_08265"
                     /pseudo
     CDS             182..311
                     /locus_tag="PK736_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582763.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     assembly_gap    312..341
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <342..566
                     /locus_tag="PK736_08270"
     CDS             <342..566
                     /locus_tag="PK736_08270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC33424.1"
                     /translation="LFVDPNFDKRGIGKRLHDMMLDWYFLRTTKTVWLGTAPGTRAEK
                     FYRTAGWREIGMHGTKEIKFEMCFEDWTRK"
     gene            complement(649..2838)
                     /locus_tag="PK736_08275"
     CDS             complement(649..2838)
                     /locus_tag="PK736_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRC33425.1"
                     /translation="MSIIRFKAVEECNSRMPVSFTAPGEKISDFFDCNVFNKEAMHKC
                     LSKEIYNQMNAATETGQRIDRKVAEQVATAMKTWAMGKGATHYTHWFQPLTGNTAEKH
                     DAFFELHDGRPMENFSSSALVQQEPDASSFPSGGIRNTFEARGYTAWDPSSPAFIIES
                     QSGKTLCIPTIFVSYTGETLDYKAPLMKALHAIDKAAVDVCQLFDKNVSKVSPTLGIE
                     QEYFLIDTALFNARPDLKSTGRTVFGHAPAKGQQMEDHYFGSIPSRVYAFMVEFETEA
                     FKLGIPLKTRHNEVAPSQFECAPIFEEINLAVDHNQLLMDMMDKVARRHHFKVLLHEK
                     PYAGINGSGKHNNWSLATNTGKNLFSPGGNPKSNLQFLTFFINTIKAVYEHADLLRAS
                     IASSSNDHRLGANEAPPAIMSIFAGAQLTAVLNDLEKKVTKEKMTPEEKTDLKIGIGK
                     LPEILLDNTDRNRTSPFAFTGNKFEFRAVGSSANCSHAMMVLNTIVANQLTSFKKEVD
                     ALINKNVNKDEAILKVLREYIVASKRILFEGNGYSEEWVKEAKKRGLSNITTTPLALD
                     AMVSKESLKLFETTGIFSHREAEARHEILLEDYIKKMEIESRVIEDLAINHIIPAAIK
                     YQTQLAQNVEAMHDIGLKANNTKVQMDMIKEISAGLMTIKTNIDGMVAERNKANNITT
                     ARAKAIAYCDKVKKYFGEIRDAADNMEVLVSDELWPLPKYREMLHIR"
BASE COUNT          798 a          554 c          575 g         1008 t
ORIGIN      
        1 aatgtgttca tttatttctt gaattagaaa aaatatgctg attggctttg attgacattc
       61 cgcattttaa ttacaatatt tatttagaac aaaatttatg atagaacaaa cagcaaaaga
      121 aaacacacca aaattgattt ttcgcgaagc taccattgaa gatattccac aagtacaaat
      181 agtgcgcaat gctgtaaaag agaatatgct ttcgaatcca aatttagtga ccgatgctga
      241 ttgtgttgaa tttattaccg tccgtggtaa gggttgggtt tgtgaaattg aaaataaaat
      301 tgttggcttt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttgtttgtt gatcccaatt
      361 ttgataagcg cggaattgga aaacgcttgc atgatatgat gctcgattgg tattttttac
      421 gaactactaa aactgtttgg cttggtactg caccgggtac acgtgccgaa aaattttatc
      481 gcaccgcagg ctggagagaa attggtatgc atggtaccaa agaaataaag tttgaaatgt
      541 gttttgagga ttggaccaga aaataaatta aatgtgtgag taatatttgt tttcataaaa
      601 aaaagccgca atcacttgcg gctttttgtc ttataaatat tttttcgttt atctgatatg
      661 caacatttcg cggtactttg gcaatggcca cagttcgtca ctcacaagta cttccatatt
      721 atcggcagca tcacgaattt cgccaaaata tttttttact ttatcgcaat aggcaattgc
      781 ttttgcacgt gcggtagtta tgttgtttgc tttattacgt tcggcaacca taccatcgat
      841 atttgttttt atggtcatca atcctgctga tatttctttt atcatatcca tttgtacttt
      901 agtattattt gctttcaatc ctatatcgtg catggcttct acattttgag caagttgtgt
      961 ttgatatttt atagcggcag gaattatgtg attgattgcg aggtcttcaa taacgcgtga
     1021 ttcaatttcc attttcttaa tataatcttc taacaaaatt tcgtgacgtg cttcggcctc
     1081 acggtgcgaa aatatcccgg tagtctcaaa tagttttaat gactctttac tcaccatagc
     1141 atctaatgca agtggggtag tggtgatgtt tgatagtcca cgtttcttgg cttcttttac
     1201 ccactcttcg ctatagccat tgccttcgaa taaaatgcgc ttggatgcta caatatattc
     1261 gcgcagcact tttaaaatag cttcatcttt attaacattt ttattgatga gtgcatccac
     1321 ttctttttta aatgatgtaa gttggttggc gactattgta tttaaaacca tcatagcatg
     1381 cgaacaattt gcagacgaac ccacagcacg gaactcgaat ttatttcctg taaatgcaaa
     1441 tggcgatgtg cgattacgat cggtgttgtc taataaaatt tcgggcaact tgccaatgcc
     1501 tattttaaga tctgtttttt cttcgggtgt cattttttct tttgtcacct ttttttccaa
     1561 atcgttgagc actgcagtta attgcgcacc tgcaaatatc gacatgatgg ccggtggtgc
     1621 ttcgttagct cctaagcgat gatcgttgct tgatgatgca atggatgcac gcaacaaatc
     1681 ggcatgttca taaacggctt taattgtatt gataaaaaaa gttagaaact gcaaattgct
     1741 ttttggattt ccgcccggtg aaaaaagatt ttttcccgtg ttggttgcaa gactccagtt
     1801 gttgtgcttg ccacttccat ttatacctgc ataaggtttt tcgtgaagca atactttgaa
     1861 gtgatgtctg cgtgccactt tatccatcat atccatcagc aactgattat gatctacagc
     1921 caaatttatt tcttcgaaaa taggtgcaca ctcaaactga cttggtgcta cttcgttgtg
     1981 gcgcgttttt aatggaatgc cgagtttgaa agcctctgtt tcgaactcta ccataaatgc
     2041 atatacgcgc gatggaatgc ttccaaaata atggtcttcc atttgttgcc cttttgcagg
     2101 tgcatgtcca aacacggtgc ggcctgttga tttcaaatcg ggacgcgcat taaacaatgc
     2161 agtatctatc aaaaaatatt cttgctctat tcctaaagtc ggtgatactt tactcacatt
     2221 tttatcgaac aactgacaca catcaactgc ggctttatct atggcgtgca gtgctttcat
     2281 caatggtgct ttgtagtcca aagtttcgcc cgtgtacgat acaaatatgg taggaatgca
     2341 aagtgttttt cccgattggc tttcaattat aaatgcgggt gatgaaggat cccaggctgt
     2401 gtacccacga gcttcaaaag tattgcgaat gccaccactt ggaaaactcg aagcatcggg
     2461 ttcttgttga acaagtgcac tactcgaaaa attttccatc ggccggccat cgtgcaactc
     2521 gaaaaaggca tcgtgctttt cggcagtgtt gccggtgagt ggttgaaacc aatgtgtgta
     2581 atgtgtagca cctttgccca ttgcccaggt tttcattgcg gtagcaactt gctccgccac
     2641 tttgcggtcg atgcgttggc cggtttcggt ggcggcattc atctggttat aaatttcttt
     2701 cgataagcat ttgtgcattg cttctttatt gaaaacgtta caatcaaaaa agtccgaaat
     2761 cttttcgccc ggggccgtga atgaaaccgg catacgcgaa ttgcattctt ctacagcttt
     2821 aaatctaatg atactcattt ttataatttt aaataatttt ggtgcaataa tactatttta
     2881 tggggggtac ttccaaatat attataatta ttttaaattt acccaccttt tttttaacgc
     2941 taggggtcat aaaatatgaa aattt
//
LOCUS       DAORQA010000216         2960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7851_length_2960_cov_5.543201, whole genome shotgun sequence.
ACCESSION   DAORQA010000216 DAORQA010000000
VERSION     DAORQA010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7851_length_2960_cov_5.543201"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2803
                     /locus_tag="PK736_08280"
     CDS             <1..2803
                     /locus_tag="PK736_08280"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33426.1"
                     /translation="ICNGSTNILDAGTFASYNWSTGSTLQTTSITLPGTYTVTVTDAN
                     GCTNSATKTVVSLLAITPVITLSPNTPVCPGSSVTLTLDSTITFSQTNPITIPNTLVN
                     ATPYPAPLVVSGFPVSGVTVKSVKLNGVSHTFPDDLDILLQSPTGTNVILMSDVGGGT
                     DIVSQNFTFKDGFPALSNNGPITGGTYAPTNVGSPDTWSAPGPGAFTQAAPALSMFNG
                     DMNGTWNLLVADGFAQDGGTINSWSITFESSNTNITYAWSPATGLSNTTQLITTATPA
                     TTTTYTVTINDGISGFTTSLSATVNVHDAPAPSISGALTFCSGSSTTLDAGTGYSGYN
                     WSTGATTQTISANTAGTFMVTVTDGNGCTGSASVSTTVNSLPTPSISGTLAFCAGSST
                     TLDAGVYSGYSWSTGATTQSISVNTAGTFTVTVTDVNGCTGSTSVTTTVNANPTPSIN
                     GTLSFCSGGSTSIDAGSYAGYNWSTGATTQTISVNTAGTFTVTVTDANGCTGSSSVTA
                     IVNANPAPSITGNLSICTGVSTTLDAGTFASYLWSDGSTTNTITTSTLGAYTVTVTNV
                     NGCSATASAIVVFGGLTPAQPGIISGPGVVCKNSAGNIYSIVAVPLATSYTWTVGPGG
                     TINSGQGSLSISVSFSNSTTASMVSVTANNNCGSSSAKTKTYTVSAIPSSPLAISGQT
                     DGVCRKINLQYSTKPVLGATSYFWTVPSGVTLNSGQGGTSIIVTYGNSFTGTGLISVN
                     AVNGCGMSAARSITVNAKPKQLVISGLNTACQFQQGLVYSVAPVNGATSYTWTPVAGS
                     SIVSGQGTTSIVYNWGSINGVISCKANNACGSSTKANFSVSFTCKESSSPFEISEVTL
                     FPNPASNITTLQFDGFAEGDATIAVYDLLGKQTMATNFSVITGVNQFPIKLDNFAKGI
                     YTVEVTLNGYSRMEKLVVQ"
BASE COUNT          884 a          629 c          613 g          834 t
ORIGIN      
        1 aatttgcaat ggttctacca atattttaga tgccggaact tttgcaagtt acaattggtc
       61 aacagggtcc actttacaaa caacatccat taccctacca ggaacctaca cagttacagt
      121 tactgatgct aacggatgta caaattcggc aacaaaaacg gtagtttcat tattagcgat
      181 aactcccgtt attacgttaa gtccaaatac accagtttgc cctggcagca gcgtaacact
      241 tactttagat agcaccatta catttagtca aacaaatcca attacaattc caaacacatt
      301 ggttaatgca acaccttatc ctgcaccact tgtggtttca gggtttcctg tttcaggtgt
      361 tacggtgaaa tcggtaaagt taaatggtgt ttcacatacc tttcctgatg acttagacat
      421 tttattgcaa tctcctaccg gtaccaatgt gattttaatg tctgatgttg gtggcggtac
      481 ggatattgta agtcagaatt ttactttcaa agatgggttc cctgctttga gtaataacgg
      541 tccaattact ggtggaacgt atgctccaac aaatgttggt tcaccagaca cctggtcggc
      601 acccggtcca ggtgcattta cacaggcagc ccctgcatta agcatgttta acggggatat
      661 gaatggaacc tggaatcttt tggtagctga tggatttgcg caagatggtg gtactataaa
      721 ttcatggtca attacttttg aaagcagcaa taccaatatc acttatgcat ggtcgcctgc
      781 aacagggtta agtaatacta cccagttaat tacaacagca actcctgcaa ctactactac
      841 ctacaccgta acaatcaatg atggtattag tggcttcaca acatcgttaa gtgctactgt
      901 aaatgtacac gatgcaccag ctccatctat tagtggtgca ttaacattct gttcaggtag
      961 ttcaacaact cttgatgcag gaaccggata cagcggttac aactggtcaa caggagcaac
     1021 cacccaaact atatcagcta acacagccgg aacctttatg gttacagtta ccgatggtaa
     1081 cggttgcacc ggttcagcat cggtttcaac tacagtaaat tcattgccaa caccatcaat
     1141 cagcggaact ttagcattct gtgcaggcag ttcaacaaca ttggatgcag gagtttattc
     1201 aggttacagt tggtcaacag gcgcaactac acaatccata tcagtaaata cagcaggtac
     1261 atttacggtt actgttactg atgtaaacgg atgtacagga agcacttcgg ttactacaac
     1321 agtaaatgca aatccaactc catctatcaa cggaacatta tcattctgct caggcggctc
     1381 aacttctata gatgcaggca gttatgcagg ttacaactgg tcaacaggag caaccacaca
     1441 aaccatttct gtaaacacag caggaacgtt tactgttacg gttactgatg caaacggatg
     1501 tacaggttct tcttcggtta ctgcaattgt gaatgcaaat ccagctcctt caattaccgg
     1561 aaatttatct atttgtaccg gagtatcgac aacactcgat gcgggtacct ttgcaagtta
     1621 tttgtggtct gacgggtcaa caactaatac aataactacc agcacattag gagcgtatac
     1681 tgttaccgtt accaatgtaa atggctgctc cgccacggca agtgccatag ttgtttttgg
     1741 tggactaaca cctgcgcaac ccggcataat cagtggcccg ggagttgttt gcaaaaactc
     1801 agcgggaaat atatacagca tagttgctgt gccgcttgct acaagttata catggacagt
     1861 agggcctggt ggcactatta actcaggaca aggttctttg tcaataagtg tgagttttag
     1921 caactcaaca acggcctcca tggtaagtgt tacggccaac aataattgtg gcagtagtag
     1981 tgcaaaaact aaaacgtata ctgtttctgc aataccttcg tcacctcttg ccatcagcgg
     2041 acaaactgat ggagtatgcc gtaagataaa tttgcagtat agtacaaaac ctgttttggg
     2101 tgccacttca tacttttgga cagtgccctc aggtgtcact ttaaactcag gccagggcgg
     2161 tacaagtatt atagttacat atggaaattc ttttactggc acaggattga tatcggtaaa
     2221 tgcggtgaac ggttgcggca tgagcgctgc aagatcaata acggtaaatg ccaagccaaa
     2281 gcaattggtc ataagcggac taaacacagc atgtcagttt cagcagggat tagtgtattc
     2341 cgtagcacct gtaaacggag ccaccagtta tacatggaca ccagttgccg gcagcagcat
     2401 tgtaagcggg cagggcacca catctatagt ttataattgg ggaagcataa atggcgtaat
     2461 ctcatgtaaa gcaaacaatg catgtggaag cagtaccaaa gccaatttta gcgtgtcatt
     2521 cacgtgtaag gaatcgtcat cgcctttcga aatttcggaa gttactttat tcccaaatcc
     2581 tgcaagcaat ataaccacac tgcaattcga tggctttgcc gagggtgatg caactatagc
     2641 tgtatacgat ttgttgggta agcaaacaat ggcaactaat tttagtgtga taacaggagt
     2701 aaatcaattt ccaattaagc tcgataactt tgccaagggt atatacactg tagaagttac
     2761 tctcaacgga tactcaagaa tggagaagtt ggtggtgcag taaataattg cgttaaattt
     2821 aataaagccg cttcttttca gaggcggctt ttttattgaa attaatattt tatttgtgcc
     2881 cgaaaaatca aaatgagact caccattttt cttatttatt tattattact gaattttaat
     2941 ctttttgcgc aggtgcctga
//
LOCUS       DAORQA010000217         2923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7948_length_2923_cov_10.705021, whole genome shotgun sequence.
ACCESSION   DAORQA010000217 DAORQA010000000
VERSION     DAORQA010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7948_length_2923_cov_10.705021"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..2786
                     /locus_tag="PK736_08285"
     CDS             48..2786
                     /locus_tag="PK736_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRC33427.1"
                     /translation="MKNLFSRLLMVMALFVITHTASASHIAGMDLTYKYISPNVYEVT
                     LKFYRDCYGIPMSGSSSVCYGSNSLGITNYVNLPAVDTNIVTPICVGQLTTCEGGTAY
                     GVEEHVYRGNVTLPQAATDWVFSFTTCCRNQAITTLQNAQAEGIWVGATLDNINFPTN
                     SSPSFTSLPVTQFCINSLNYFPQYASDIDGDSLYFALVDPYSDGGTCTTPGNPVLFNS
                     PFSGINPLTTSAPFTVLDPFTGLITLTPILFEIGVMALDLMEYDSVGTLKSTIRRDIQ
                     VSVVQCNFAQFQTTIVNGVPKAYAANCGDSCVTLQLTDSIQCSTVAPNGSDFRAVRPN
                     GQPNPIYSATPLNCFNGLTTQIQICFYKPLTQGTSKFWLKTGNDLNTLLPSCGNALPE
                     YDTINIVVFDPSIMALPVIDTVPSCAFTTYTITMPELITCASIAANGSDFILKDATGT
                     PYPVTSASGVNCSFQNEFTNQITITLNAPAQGTPPYYLIATNGNDGNTITNECDKPID
                     QNDTIAILYTTNTVTVSLGTDTTLCQNDPAPVLDATLTLATYTWYLNGAPVGGNTQTL
                     TANQSGTYICQVSYGPNCFGSDTVVVNYVLAPVVALGNDLAVCNAAPFPTLDAGNPGS
                     TYAWTDINGNPLATTQTYTPTAAGTYIVTVSGGSCSSSDTINVTVSNATAFTLGNDDV
                     ICETASTTITSSVAGSSYVWFKDGTNLNTNAQTITVNAAGTYVCELTNTDGCKSTDTI
                     IVTTEALPAKPQVFCGVATGTGFSIVYTWAPIAGATGYAVSVNGAPFVPNNLLPVSHG
                     LNSSPASFIVHALGTECEFGPNSDPAFCDISIPNIVTPNNDGKNDAFVILNLEQFSNA
                     RMKIYNRWGNLIYENANYKNDFNFQDQPDGVYYYVLTISEVSKDYTGNVTVYKEKK"
BASE COUNT          880 a          622 c          553 g          868 t
ORIGIN      
        1 cctcactata aaaaaataaa atttaataaa ctgatataat taaaactatg aagaatttat
       61 tttctcgatt gctaatggta atggcgttat ttgtaataac ccacaccgcg tctgcctcgc
      121 atattgcagg tatggactta acctacaaat acattagtcc taatgtgtat gaggtaacac
      181 ttaaatttta ccgcgattgt tatggtatcc caatgtcggg gtcgtcaagc gtttgttatg
      241 gttcaaactc tttgggtata acaaactatg tgaacttacc tgcggttgat acaaatattg
      301 taacgccaat atgtgtgggt caacttacaa cttgtgaagg aggaactgca tacggtgtag
      361 aagaacacgt atacagaggg aatgtaactt tgccacaggc cgctaccgat tgggtatttt
      421 ctttcaccac ttgctgccgc aatcaagcca ttactacttt gcaaaatgcg caagccgaag
      481 gtatatgggt tggtgctact ttggataata taaattttcc taccaatagc tcaccatcct
      541 ttacttcgtt gccggtaacg caattttgca taaatagctt aaactacttt ccacagtatg
      601 cttccgatat tgatggcgac tctctttact ttgcattggt agatccttat agcgatggtg
      661 gtacttgcac cactccggga aatccagtat tgttcaattc acctttttcg ggcataaatc
      721 cgttaactac atcagcgcct ttcaccgtgc tcgatccatt tacaggtctt atcaccttaa
      781 cacctattct tttcgaaatt ggggtaatgg cacttgacct gatggagtat gacagtgtag
      841 gaacattgaa aagcaccatt agacgcgaca ttcaggtatc agtagtgcaa tgtaattttg
      901 cacaattcca aactacaata gtaaatggtg tgccaaaagc ttatgctgca aattgcggtg
      961 attcatgtgt gactttacaa ttaactgata gtatacaatg cagtactgtg gctccaaacg
     1021 gttccgactt tagagctgtg cgtccaaacg gacaaccaaa ccctatttat agtgctacac
     1081 cacttaactg cttcaatggc cttacaacac aaatacaaat ttgtttttac aagcccctta
     1141 cgcaaggcac atcaaaattc tggctcaaaa caggaaatga tttaaataca ttattgccat
     1201 cgtgtggtaa tgccttaccg gagtacgata caataaatat tgtggtgttt gatccttcca
     1261 taatggcttt acctgttatt gataccgtgc cctcgtgcgc atttacaact tatactatca
     1321 ctatgcctga gttaattact tgtgcatcta ttgctgcaaa tggttctgac ttcattttga
     1381 aagatgctac cggaactcct tacccggtta catcggcaag tggcgttaac tgctcattcc
     1441 aaaatgaatt tacaaatcaa attactatta ccttaaatgc accggcacaa ggaactcctc
     1501 cttattacct tattgctacc aatggaaatg acggtaatac tattaccaat gaatgtgata
     1561 aaccgataga ccaaaacgac actattgcca ttctttacac taccaataca gttacagtaa
     1621 gtttaggcac cgatactaca ctatgccaaa acgatccggc tccggtttta gatgctacat
     1681 taacactggc aacttatacc tggtatttga acggtgcacc tgttggaggc aatactcaaa
     1741 cattaacggc taaccaatct ggtacgtaca tttgtcaggt ttcttatggc cctaattgct
     1801 ttggctccga tacagtggta gtaaattacg tattagcgcc tgtggtcgcg cttggcaatg
     1861 atttagcagt gtgtaatgcg gctccattcc caactttaga tgccggtaat cccggatcta
     1921 cttacgcatg gacagacatt aatggtaatc cattggcaac tacacaaacc tatacaccaa
     1981 ctgcagccgg tacgtatatt gttaccgtta gcggaggctc ttgctcttcg agcgatacaa
     2041 taaatgtaac tgtgagcaat gcaactgcgt tcaccctggg caacgatgat gtaatttgcg
     2101 aaacagcatc tacaacaata acgtcaagtg tagcaggttc ttcttacgtt tggtttaaag
     2161 atggaaccaa cctcaacaca aatgcgcaaa caattacagt aaatgccgca ggcacatatg
     2221 tatgtgaatt gacaaataca gatggttgca aatcgaccga tacaataatt gttactacag
     2281 aggcattgcc ggcaaaacct caagtgtttt gcggtgttgc caccggaaca ggatttagca
     2341 ttgtatatac atgggcgcct atagccggtg ctacaggtta tgcagtaagt gtaaatggag
     2401 caccatttgt tccaaacaac ttattgccgg taagccatgg tttaaatagc tcacctgcct
     2461 cgtttattgt acatgcctta ggcacagagt gcgagtttgg accaaacagc gatcctgcct
     2521 tctgcgatat cagtattcca aatattgtaa caccaaacaa tgatggcaag aacgatgcat
     2581 ttgtaatatt aaatcttgag cagttttcta atgccagaat gaaaatatac aatcgttggg
     2641 gtaatttgat ttacgaaaat gccaactaca aaaacgattt caacttccag gatcagcctg
     2701 atggagttta ctattacgtg ttaactattt cggaagtgag taaagactac accggaaatg
     2761 taaccgtata taaagagaag aaataaacct cgctccagta ttttttaaaa tactgattac
     2821 aaaaaacgct tgcagatata aactgcaagc gttttttttt gatagatgcc ttatctaccg
     2881 gttctttttc atcttacgtt tggtctaata attcagcaaa ctc
//
LOCUS       DAORQA010000218         2922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7952_length_2922_cov_10.633764, whole genome shotgun sequence.
ACCESSION   DAORQA010000218 DAORQA010000000
VERSION     DAORQA010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7952_length_2922_cov_10.633764"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2922)
                     /locus_tag="PK736_08290"
     CDS             complement(<1..>2922)
                     /locus_tag="PK736_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33428.1"
                     /translation="VFLECIIPSKDGGFVISGSSNSNISGDKTENNWDTTLATRDYWV
                     MKIDSIGNIVWQNTIGGTMEDFTASINATTDGGYIIGGTSKSDSSGDKTENGCNTFNG
                     SWDYWVVKIDSIGNILWQNTITANFTDRMYGISQTTDGGFICGGTSHSNISCDKIENN
                     LDYPHNSTSDYWVVKLDSIGNILWQNTIGGGQWCSDHLSSIELTKDGGYICGGSSDGT
                     WAGNDKSEMTKGGYDYWVVKLDSVGNVEWENTIGGASVDYLYSVKQLIDGSYICGGTS
                     KSNVSSDKANPSIGGYDYWIVKLDAFGDIVWQKTIGGNSDDDLDYITPTIDNGFICGG
                     ASISSISGLKTEVNLGSGDNWIIKLDSNGNIQWQNTIGGSGNDGLARVFPSYDGNSII
                     CGATSNSGISGDKTENSKGGYDFWIYKINDIEYGSLITGNAFVDNNANSIQDSSENNL
                     QYKRIDLLSHNLWTYTAANGFYNLPVPDSGNFTITPANVNYFTPNPNFHTATFTGTPK
                     LDSLNDFPFQPIGIVNDLQIAVTPISAFRANSNCNYKLDYSNKGNTTLNASIIFYLGD
                     TNYNYVSASVAPSLINSDSIVWNNITLTPWQQENIVVTVYVKNGVAIGTQIFSLCTIN
                     PIVGDSVPGDNYGSWKVITTGSFDPNNILVDEDTLITNQLADGASLNYIINFQNTGND
                     TAFYVKVLNPIDTNRFDLNTFEIVSSSHPFILNYIPWENNMEFKFENILLPDSNIDEA
                     NSHGYLCYRIKPKTTLPPNDSLTNFASIYFDSNSPIRTNNAVTRILPPNVYIHLYATV
                     CDNYVSPSGKYTWANSGIYLDTIPVGIGLDSIFIVHLTLNKSFAVIDTNDCENYVSPS
                     GNYLWTTSGIYSDTLINALGCDSIITIKLTINKSNSIINVTKCDSLTSQISNYVWTTS
                     GTYFDTIPNAKGCDSLITINLTIQQPFQINSTILMCQGDSTFLSGSWQTIAGFYFDSL
                     QS"
BASE COUNT          951 a          548 c          479 g          944 t
ORIGIN      
        1 gattgtaagg aatcaaaata aaatccagca attgtttgcc atgagccgga gaggaaagta
       61 ctatcacctt ggcacattaa aatagttgaa ttaatttgaa agggttgttg aatagtaaga
      121 ttaattgtaa ttaatgaatc acaaccttta gcattaggaa tggtatcaaa ataagtaccg
      181 cttgttgtcc aaacatagtt actaatttgc gatgtcaagc tatcgcactt ggttacattg
      241 ataatagaat ttgatttatt tattgtaagc tttattgtaa taatagaatc gcaacccaag
      301 gcatttatta atgtatcaga ataaattcca gaggttgtcc ataaataatt tccgcttggt
      361 gaaacataat tttcgcaatc gttcgtgtca ataacagcga acgatttatt caaagtaaga
      421 tgcactataa atattgaatc caacccaatc cccaccggaa tggtatctaa ataaattccc
      481 gaatttgccc aagtatattt acccgatgga gaaacgtaat tgtcacaaac agtagcgtag
      541 agatgaatat aaacatttgg aggtaaaatt cttgtcacag cattatttgt tcttattggg
      601 ctgttagaat cgaaatatat acttgcaaaa tttgttagac tatcatttgg aggcaaggtt
      661 gttttgggct taatgcgata gcataaatac ccatgactat tggcctcatc aatattacta
      721 tcgggtaaaa gaatattttc gaacttaaat tccatgttgt tttcccaagg tatataattc
      781 aaaataaaag gatgcgatga cgacacaatt tcaaaagtgt ttaagtcaaa tcggttggta
      841 tcaatagggt tcagaacttt cacataaaat gctgtgtcat taccggtatt ttgaaagttg
      901 attatgtaat taagagaagc accatcagca agttgattgg taattaatgt atcctcatca
      961 acaagaatat tatttggatc aaaactgccg gttgtaatta ctttccacga cccatagtta
     1021 tcaccgggaa cactatcacc aacaattgga ttgatagtgc aaagtgaaaa tatttgagtg
     1081 ccaatagcta caccattttt tacatagacg gtgactacaa tattttcttg ttgccatgga
     1141 gttaatgtaa tgttgttcca cacaatcgaa tcagagttga tgagcgaagg ggcaacactt
     1201 gcacttacat aattataatt tgtatcgcca agataaaata ttatagatgc atttaatgta
     1261 gtgttacctt tgtttgaata atctaatttg tagttacaat tactgtttgc gcgaaaagcg
     1321 cttataggtg ttacagcaat ttgcaaatca ttcactatgc caatgggctg aaaaggaaaa
     1381 tcgttcaatg aatctaattt aggcgtacca gtaaatgttg cagtgtgaaa atttggattg
     1441 ggagtaaaat aatttacgtt agccggtgta atcgtaaaat taccactatc agggactggc
     1501 aaattataaa aaccgtttgc agcggtatat gtccacagat tatggcttaa taagtcgata
     1561 cgtttatact gcaaattatt ttctgaagaa tcttgaatgc tgttggcgtt attgtcaaca
     1621 aaagcattgc cggttatcaa actaccgtat tctatatcat ttattttata aatccaaaaa
     1681 tcgtaaccac ccttgctgtt ttcagtttta tcaccggaaa taccggaatt agaagtcgca
     1741 ccacaaataa tgctgttacc atcataagaa ggaaatacac gtgccagacc atcattgccg
     1801 ctaccaccaa ttgtattttg ccattgaata tttccgtttg aatctaattt aataatccag
     1861 ttatccccgc tacctaaatt gacttctgtt ttcaggcccg aaatggatga aattgaagcg
     1921 cctccgcaaa taaacccatt atcaattgta ggtgttatat aatccaaatc atcatcgcta
     1981 tttccgccaa tagttttttg ccaaactatg tcaccaaaag catctaattt cactatccaa
     2041 taatcataac caccaattga tggattcgct ttatcgcttg atacattgga tttagatgtg
     2101 cccccacaaa tataacttcc atcaataagt tgttttacgc tatataaata atctacgcta
     2161 gcaccaccaa tagtgttttc ccattctaca tttccaacag aatcaagctt aacaacccaa
     2221 taatcgtatc ctcctttagt catttcagat ttatcatttc ctgcccaggt tccatcagaa
     2281 gaaccaccgc atatataacc gccatctttt gttagctcga tagaactcaa atggtcgctg
     2341 caccattgtc caccacctat agtattttgc catagaatat ttccaatcga atcaagtttt
     2401 acaacccaat aatcacttgt gctattgtgt gggtagtcta aattgttttc tattttatca
     2461 caggaaatat ttgagtggga tgtgccacca caaataaagc ctccatctgt tgtttgagaa
     2521 atgccataca ttctatccgt aaaattagcc gtaattgtat tttgccatag aatattacct
     2581 attgaatcaa tctttactac ccaataatcc caagaaccat tgaaagtgtt gcaaccattt
     2641 tcggttttat ctccacttga atcagatttt gaagtgcctc caataatgta accaccatct
     2701 gtggtagcat ttatcgatgc ggtaaaatcc tccattgttc cacctatagt attttgccaa
     2761 acaatgttac caatcgaatc aattttcatg acccaataat cgcgtgtggc aagggtggta
     2821 tcccaattgt tttctgtttt atcaccagat atatttgagt ttgaactacc gctgattaca
     2881 aaacctccat cttttgatgg tattatacat tcgagaaaaa ca
//
LOCUS       DAORQA010000219         2916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_7975_length_2916_cov_9.200979, whole genome shotgun sequence.
ACCESSION   DAORQA010000219 DAORQA010000000
VERSION     DAORQA010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7975_length_2916_cov_9.200979"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PK736_08295"
     CDS             complement(<1..912)
                     /locus_tag="PK736_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019990721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC33429.1"
                     /translation="MTENIKLGLKENWKQFTILVIVNAFVGGMIGMERTIFPQFAELE
                     FGVASKTAILSFITAFGITKAIANYFTGRLANKFGRKNLLLFGWLIAIPIPFILIYAP
                     SWGWVIFANVLLGVSQGLTWSSTVVMKIDLVGEKDRGFAMGLNEFAGYFAVGIVAFLT
                     GYIANTYGITPYPFYIGIFISIVGFILTALWVKDTRSFVQKESATENTSQLDNVFLET
                     TLKNKTLSSVTQAGLVNNLNDGMIWGLLPIVLFSLNFDNANIGIITAIYPTVWGIGQL
                     FTGKMSDHYSKKAMLFWGMLLQGVAILL"
     gene            1723..2853
                     /gene="dprA"
                     /locus_tag="PK736_08300"
     CDS             1723..2853
                     /gene="dprA"
                     /locus_tag="PK736_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRC33430.1"
                     /translation="MTTHHQDTNTEELKYKIATTLIAGVGNMLAKTLVSYCGSFEAIF
                     KEKKSHLEKIPGVGPVTCKNILAFKDFDRTEKEITFINKYKIQAWSYLDKQYPDKLRQ
                     CFDSPVMIYFKGNCDFNLQPCLAFVGTRKATDYGKQLCEEIIEALIPYNVNIISGLAY
                     GIDICAHKQALKSDITTVGVLAHGIDKIYPPAHRSVAEKMLHNGGLITEYISETNPDR
                     ENFPTRNRIIAGLCNATIVIEAGERGGALITAEIANSYNRDVFAIPGRSTDEYSKGCN
                     RLIKLQKAQMVESAADICYQLGWQNDAKAEKEKHKQVKLFIDLTPDEKTIYDFLINND
                     QNGIDNICIACEMSTSRTAAALLSLEFSGLVKSMPGKIFKSL"
BASE COUNT          927 a          589 c          541 g          859 t
ORIGIN      
        1 aagtaaaatt gctacccctt gtaatagcat tccccaaaat agcattgctt tttttgagta
       61 atggtcagac attttgcctg taaaaagttg tccgataccc caaacagttg gataaattgc
      121 tgtgattatt ccaatatttg cattgtcaaa atttagcgag aaaagaacta ttggaagcaa
      181 gccccaaatc attccgtcat ttagattgtt tacaagtcct gcctgtgtta cagaacttaa
      241 tgttttgttt tttaatgtcg tttctaaaaa cacattgtca agttgtgatg tattttcagt
      301 tgcactttcc ttttgaacaa atgaccttgt gtcttttacc caaagtgcag ttaagataaa
      361 gccaacaatg gaaatgaaaa ttcctatgta aaatggataa ggtgttatac cataagtgtt
      421 ggcaatataa cctgtcaaga atgcgacaat accaactgcg aaatatcctg caaattcatt
      481 taatcccatt gcaaatccac ggtctttttc gccaacaagg tcaattttca ttactacggt
      541 gctactccaa gtcagtcctt gactaacacc taaaagcaca ttggcaaaaa ttacccaacc
      601 ccaacttggg gcataaatga gtataaacgg aattggaata gctatgagcc aaccaaatag
      661 aagtaagttc tttcgtccga atttattggc aagtcgtccg gtgaaataat ttgcaattgc
      721 tttggtaatt ccaaacgctg taataaaaga aagaatagct gtctttgaag ctacgccaaa
      781 ttccaattcg gcaaactgcg gaaaaatagt ccgttccatt cctatcattc cgccaacaaa
      841 agcgtttacg attaccaaaa tggtaaattg cttccagttt tctttaagtc cgagttttat
      901 attttctgtc attccttttt attgtaaatt tccattttaa aatcctttta gtttgttaca
      961 aaagtcactg tttatttttg ggttgtgtcg ttcgttacgc ttgcacctga cggcagcctg
     1021 tgaaaaaacc atttcctcaa cagttgcacg ctaacagcat gtggtaaaaa taaaaattac
     1081 aacgacactg ttgacatacc ttagcaaaaa aaatagttat gcatcgcata gctgagggct
     1141 gtaggccaat gtcattaagt taagccttcc gcgcaccctg aatccctaaa gggagccagt
     1201 ccgttgtaac gaaacctgtg tggaggcgcc ctttagggct acgggtgagc gcaagcaatt
     1261 ggatttaatt ccttaactta atgacattgg gctaaagcct gatgcaaaat tatttatacc
     1321 gtccgataaa tcggacggca atgatttgct tacacaaaaa ggtttgcgac tttgagtctt
     1381 ggtggcaaaa aaaatatccg gcattcattc gcgtattccc cgacactgtg gtcggggcag
     1441 gcgtggcaaa aaagtattta ggctaaagcc agtataaatt atttaaaccg tccgataaat
     1501 cggacggcaa tgaatgcttg cgcacgaaga acatgacccg ctttagagtc ctcattagct
     1561 tttgtttttt tttggtatga gagactcgaa agctagacta aaaactcggc attcattcgt
     1621 cctattcgcg gcaagaaaat attatgtgtt ttcagtggca ttaaattttt attttggttc
     1681 ggcatacaat tattctttta ttcgcaatcg aagcattatc aaatgacaac tcatcatcag
     1741 gatacaaaca ccgaagaatt aaaatacaaa atagcaacaa cactaattgc aggcgtaggt
     1801 aatatgcttg caaaaacatt ggtgagctat tgtggaagtt tcgaagcaat attcaaagaa
     1861 aaaaaatctc atctcgaaaa aattcccgga gttggcccgg ttacttgtaa aaatattttg
     1921 gctttcaaag atttcgatcg taccgaaaaa gaaataacct ttatcaacaa atataaaata
     1981 caggcatgga gttatctcga caaacaatat cctgataagc ttcgtcaatg cttcgattca
     2041 ccggtgatga tttattttaa aggcaattgc gatttcaatt tacaaccatg ccttgcattt
     2101 gtaggtacgc gcaaagccac cgactatggc aaacaacttt gcgaagaaat tattgaagca
     2161 ctcattcctt acaatgtaaa tattattagc ggccttgctt acggcataga tatttgcgca
     2221 cacaagcaag cactaaaaag tgatatcact actgttggtg tgcttgcaca tggcatcgat
     2281 aaaatttacc ctccggcaca tcgtagtgtt gccgaaaaga tgctgcataa tggtggcctc
     2341 atcaccgagt acataagcga aacaaatccc gatcgcgaaa attttcctac gcgtaaccga
     2401 atcattgccg gcctatgcaa tgccactatt gtaattgaag ctggcgaacg tggtggcgca
     2461 cttatcactg ccgaaattgc taattcctat aaccgcgatg tatttgcaat tcccggccgc
     2521 agcaccgatg aatatagtaa agggtgtaac cgccttatca aattgcaaaa agcgcaaatg
     2581 gtagaatcgg ctgccgatat ttgttatcag ctcggttggc aaaacgatgc caaagccgaa
     2641 aaagaaaaac acaaacaagt aaaacttttt atcgacctca ctcccgatga aaaaactatt
     2701 tatgatttcc tcatcaacaa cgaccaaaat ggaattgaca acatttgcat tgcatgcgaa
     2761 atgagtacga gtcgcaccgc agccgccctg ttaagtttag agttttccgg tctggtaaaa
     2821 agtatgccgg gtaaaatttt caaaagtttg tagctatttc tttttatttt aaaattcttg
     2881 tcattgttta atacatgtca ttggtcattt taaacc
//
LOCUS       DAORQA010000220         2901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8030_length_2901_cov_4.921644, whole genome shotgun sequence.
ACCESSION   DAORQA010000220 DAORQA010000000
VERSION     DAORQA010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8030_length_2901_cov_4.921644"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PK736_08305"
     CDS             <1..1033
                     /locus_tag="PK736_08305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HRC33431.1"
                     /translation="DSKIHARFSLYPPTNALLLFPLNTFSIYAAKNIWLAVSVACTFL
                     SILFFSRISLLNISSSAVIVLLSGFNLCNDFYLGQIYSFLLMLTLCGLYYMTRKPFLS
                     AIFISLVASLKLITLGFLLLLVRKKYIPSAILFAIILTGITFLSAYMGGFQVFANYVS
                     QRLIPLSFGNIFNELPFSVQYQSVISLLSNCFVYNAEYNPNPVFPEYNNLFLPCKYLF
                     YGIMVCVLGLNLRWFISKKIDGSAFISLSILILLLLESGSATYHLLFLLPVLSKVLAD
                     VNFIASQKIILIVLLAAIGFAPTMINSVFDLSYLPFILKYPRLLLMITYFCFVQYLYF
                     QRNKKSIII"
     gene            1037..2182
                     /locus_tag="PK736_08310"
     CDS             1037..2182
                     /locus_tag="PK736_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33432.1"
                     /translation="MKTYLKICILFGSILIFLSSYREDDTTIKLKWNKAYAGETRDTV
                     TTGLLWSLSLLGAELPLGCKDDGIEWNDSNLITLHLHKMGFAETSLKFLKVLCDSIKI
                     SDEYKLTGGIDIGRWIVLTLHSPWHYYKITGTAPTLQEFYNRHSIDSNIFFVVCNSGV
                     AKKPRRIKMEVGSKINDLCFVAEQSNDSNCNVNFTAIEYETFDVMPNSQLRFAIYDRN
                     GNLKDASDTTNSHAGKPGKCMWCHESKVQPLFFRTEETNSNLTLKDFTKMVDSANAII
                     DTYRKNNLCEINFDNKQDHTLHELLYISFMEPSAGRTSLEWLNFNSDKNLQSLANTQN
                     HIYEEFPFLGKLYWRSTIDSISPFKVMAVPNSVREPSVYEPNYFKNY"
     gene            2191..>2901
                     /locus_tag="PK736_08315"
     CDS             2191..>2901
                     /locus_tag="PK736_08315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC33433.1"
                     /translation="MKYHVLHIARWYPNLTDKQIGSFVKKHIEAISLKNKSTLIYVQR
                     DPLLQTAFKIEKQQQENFISYILYFRKTNFSILNLLRYFFYHFKLLKIVIKEQGKPNI
                     VHLHAMYGMSLITYLLSQKLHVPFFITEHWNGFTNDKFSKKDFVFRWVTKYVSKKAKG
                     ITAVSSFLQKGLKENNISESIVVIPNVVEKFEGAPKLKFDNTKINILTIADLDDDVKN
                     ISAIITALSNIKNETKNNF"
BASE COUNT          913 a          505 c          472 g         1011 t
ORIGIN      
        1 agattcaaag attcatgccc gattttcttt gtatcctcct accaatgccc ttctgttgtt
       61 tccactaaat actttttcca tttatgcagc aaaaaatatt tggctggcag tgtcagttgc
      121 atgcacattc ttgagcatcc tttttttctc acgtatatca cttttaaata ttagctcaag
      181 tgcggttatt gttttgctga gtggtttcaa tttgtgtaac gatttttatc tcggtcaaat
      241 ttattctttc ttgcttatgc taaccttatg cggattatat tacatgaccc gaaagccatt
      301 tctttctgcc atctttattt cattggtggc atcgttaaaa ttaatcactt tgggattttt
      361 gcttttatta gttcgcaaaa aatatattcc atctgctatt ttatttgcaa tcattcttac
      421 tggcattact tttctttcgg cttacatggg cggttttcag gtttttgcaa actatgtttc
      481 acaaagactt attccacttt cttttggcaa tatttttaat gagctaccat tttctgttca
      541 atatcaatcg gtaatttctt tgctttcaaa ttgtttcgtt tacaatgctg aatacaatcc
      601 caatccggta tttcctgaat acaataattt atttttgcct tgtaaatatt tgttttacgg
      661 cataatggtt tgcgttcttg gtctaaattt gcggtggttt atttcaaaaa aaattgatgg
      721 ctctgcattt atatcgctta gcattttgat tttactttta ttagagtcag gctcggcaac
      781 ttaccatctg ttgttcctac ttcctgttct ttctaaggtt ttggctgatg ttaatttcat
      841 tgcttcccaa aaaatcatac ttatagttct gcttgctgca attggttttg ctcctacaat
      901 gattaattcc gtttttgatt tatcttattt gccattcatt ttaaaatacc ctcgcctact
      961 attgatgatt acttattttt gctttgtgca atatttgtac tttcaacgca acaaaaaatc
     1021 tatcattatt tagtgtatga aaacatactt gaaaatttgt attctatttg gttcaattct
     1081 tatatttctt tcatcgtacc gcgaagatga cacaaccata aaattaaaat ggaacaaagc
     1141 atatgcaggt gaaacaaggg atacagtcac taccggatta ttgtggtcac tttctctgct
     1201 aggcgctgag ttacctttag gttgcaaaga tgatggcatc gaatggaatg attcaaattt
     1261 aataacactg catttgcata aaatgggatt tgccgaaacg agcttaaaat tcttgaaagt
     1321 gctttgcgat tcgataaaaa tatcggatga atataaatta actggcggca tcgatattgg
     1381 cagatggata gtgcttacat tgcacagccc gtggcactat tataaaatta ccgggaccgc
     1441 accaactttg caggaatttt ataatcgcca ttcaatcgat tcaaatattt tctttgtggt
     1501 gtgcaactcg ggtgtggcaa aaaaaccacg aagaataaaa atggaagttg gaagtaaaat
     1561 aaatgatttg tgttttgttg ctgaacaatc aaacgatagt aattgcaatg taaactttac
     1621 cgctatagaa tatgaaacat ttgatgtgat gccgaactct caattacgtt ttgctattta
     1681 cgatcgaaat ggcaatctga aagatgcatc ggacaccaca aactcacatg caggcaagcc
     1741 cggaaaatgt atgtggtgcc acgaaagcaa agtgcaacca ttattttttc ggacagaaga
     1801 aaccaattcg aatttaactt tgaaagactt taccaaaatg gtagattcgg caaatgcaat
     1861 tattgataca tacagaaaaa ataatttgtg cgagattaat tttgataata aacaggacca
     1921 tactttgcac gaattacttt atatttcttt tatggaacct tctgctggaa gaacttcgtt
     1981 ggagtggttg aattttaatt cagataaaaa tctgcaatcc cttgcaaaca ctcaaaacca
     2041 tatatatgag gaatttccat tcttaggcaa attatattgg cgcagcacta ttgatagtat
     2101 atccccgttt aaagttatgg ctgttccaaa ctcagttcgc gaaccatcgg tgtatgagcc
     2161 aaactatttc aaaaattatt aaatttaaaa atgaaatatc atgttttaca tattgcccga
     2221 tggtatccca atttgactga taagcagatt ggaagctttg taaagaaaca catcgaggct
     2281 ataagcctta aaaataaaag cactttaatt tatgttcaac gcgatccatt attgcaaaca
     2341 gcttttaaaa tagagaagca acaacaagaa aattttattt catacatctt atattttcgc
     2401 aaaaccaatt tttcaatttt aaacttgcta cgctattttt tttatcactt taagctgttg
     2461 aaaattgtta taaaagagca aggcaagcct aacatagttc acttacatgc aatgtatggc
     2521 atgtcgctta taacttatct tttatcacaa aaattgcacg tgccgttttt cattaccgaa
     2581 cactggaacg gatttacaaa cgacaagttt tcaaagaagg atttcgtatt ccgttgggta
     2641 acaaagtatg tgagtaaaaa agcaaaagga attactgctg tgtcatcttt tttacaaaag
     2701 ggattgaaag aaaataatat ctccgaaagc atagttgtaa ttcccaatgt tgtcgaaaaa
     2761 ttcgaaggcg cgcccaaatt gaaatttgac aacacaaaaa taaacattct cactattgct
     2821 gatttagacg atgatgtaaa aaatatttcg gcaatcataa cagcgttaag caacattaaa
     2881 aacgaaacga aaaataattt t
//
LOCUS       DAORQA010000221         2892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8054_length_2892_cov_9.344025, whole genome shotgun sequence.
ACCESSION   DAORQA010000221 DAORQA010000000
VERSION     DAORQA010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8054_length_2892_cov_9.344025"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2892)
                     /locus_tag="PK736_08320"
     CDS             complement(<1..>2892)
                     /locus_tag="PK736_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33434.1"
                     /translation="IKVNGEVNVVISDGAGGYYVGGSFTAGGKPNLIRVNSSKAINAT
                     FTPNPNGAVYDLKLVGNRLYVCGAFTIMSGVTRRYIAAINKSTSAALPTDPNLNNAAY
                     DLKLIGDTLYFGGSFTTVGTTQQSGIGALIASTGANVTINSIDGPVYSIAADTINKEL
                     YFGGYFNSIGQNKRGCAKISSTTGLSNPSFPGVVGTIYTCITDSNGGWYIGGSFSSTV
                     GASNLAHITSGNVIDAAFKPNPNSTVYALALTGSKLYAGGSFTSIGGQARLRLVQLNK
                     TSGVVQTWDPGCDGTVYSLTLNDTLIYATGQFNTVNAVTVLNYAAINVANAKVYRSVG
                     TNGIVRTSIQDANNLYLGGAFSLIGKPTAGIASYTTTSSNPNLNFYGINGTINTIISD
                     GGTGWYVGGYFTAPNGYTHLVHILASGAVDAAFDPNPNYTVNCLLLNGNNLYVGGSFN
                     QIDLQARSYIAAVNKATGALIPAFNVTLNNNPNCFLLSGTNLLIGGEFTTINGTTRNR
                     IGSVALSNGSVQTWDPNSNSAVYTLVLNGTTVYAGGAFTNIGGAARNYIAALSTTTNN
                     ATAWNPNCNSYVYTLALSGTTLYAGGIFTTINGLGRNRAAGININNASITAFNPDLNN
                     YVRAINISGTTIFIGGQFTTAGGQPRYYLASFSTAGAVQAWSPNVDNYVYTIGINGTN
                     VLAGGSFRLSNLLAQNALVKIAKANYTATTWNPNANVGADVYATALNGTVLYVGGTFS
                     SLGGVLRSNLGAVNTNNNNATTWNPAPNSSVYSLHFKSNLIYVGGAFTIINNTARNYV
                     AAVNNTNVLQTWNPAPNYYVRSIGTYTTDVALGGEFTFVNNIFRTNLLAMKHSDFSIK
                     SYNAAMPSGQIYELWHDGSKLYVGGTFSQIGGQARANLASLNLTNGNASSWNPIVNSP
                     VYDLVRQGNYIYIGGPFTLVNNVARSYAAAIGISGTGTLSNWMPQYNNT"
BASE COUNT          842 a          627 c          560 g          863 t
ORIGIN      
        1 cagtattatt atattgaggc atccaattgg ataaagtgcc tgtgcctgaa ataccaattg
       61 ccgcagcata actacgtgca acattattta ccaatgtgaa aggcccacct atgtaaatgt
      121 agttgccctg ccttaccaaa tcgtatacgg ggctatttac tattggattc catgacgaag
      181 catttccatt tgtaaggttg agcgatgcta aattggcgcg tgcctgtcca ccaatttggc
      241 taaatgtacc gccaacatat agtttactgc catcgtgcca tagctcatat atttgtccac
      301 ttggcattgc agcgttgtaa ctttttatgg aaaaatcgct gtgcttcata gccaataaat
      361 ttgtgcggaa aatattattt acaaaagtga attcgccacc aagcgcaaca tcggtagtgt
      421 atgttccgat agaacgcacg taataatttg gagccggatt ccacgtttgc aatacattgg
      481 tattatttac tgcggccaca taatttcgag ctgtgttatt gataatggta aaagccccac
      541 ctacatatat caaattgctt ttaaaatgca aactatacac actgctgtta ggtgccgggt
      601 tccatgtggt agcattgttg ttgtttgtat ttactgcacc taagttactg cgcaacacac
      661 caccaaggct cgaaaatgtt ccacctacgt ataacaccgt accgtttaac gctgttgcat
      721 aaacatcagc acctacattg gcatttgggt tccaggtagt tgcagtgtaa tttgctttgg
      781 caatttttac caacgcgttt tgcgccaata aattcgacaa tctaaaactg ccaccagcca
      841 gcacattagt tccatttatt cctatagtat aaacgtaatt atccacattt gggctccatg
      901 cctgaacagc accggcagta gaaaacgagg ctaaataata tcgtggctgc ccacctgcag
      961 tggtaaattg tccgccaata aaaatagttg tacctgaaat atttatagcg cgtacataat
     1021 tatttaaatc gggattgaaa gccgtgatac ttgcattgtt aatgttgata cctgctgcgc
     1081 gattacggcc aagtccgttt atggttgtaa atattcctcc ggcatataaa gtagtgccac
     1141 taagcgccaa tgtataaaca tatgaattgc aatttggatt ccatgctgtt gcattgtttg
     1201 tggtagtgct caatgcggca atataattac gtgcagcacc tccaatatta gtaaacgctc
     1261 cgcctgcata tacagttgta ccattcaata ccaaagtata taccgcgctg ttgctgttag
     1321 ggtcccatgt ttgtacactt ccattgctca atgccaccga acctattctg ttgcgggttg
     1381 taccattgat ggtagtaaat tctcctccta ttaataagtt tgtgcccgat aataaaaagc
     1441 aattcggatt attatttaaa gtaacattaa acgcaggaat caatgccccc gtggctttgt
     1501 taactgctgc tatatacgac cgtgcctgta aatctatttg attaaaactt cccccaacat
     1561 acaagttgtt tccattaagc aataaacaat ttacagtgta gtttggattg ggatcgaatg
     1621 cagcatccac agcaccactt gctaatatgt gcacaaggtg cgtatatcca ttaggggcag
     1681 taaaatatcc gcccacgtac caacctgtgc cgccatcact tatgatagta tttatagttc
     1741 cattgatgcc ataaaaatta agatttggat ttgagctggt agttgtatat gatgctatac
     1801 ctgctgttgg cttgcctatc aaagaaaacg caccgcccaa atataagttg ttagcatctt
     1861 gaattgatgt acgaacaatg ccgttagtac ccaccgacct gtaaaccttt gcgttggcaa
     1921 catttatagc ggcatagttg agaaccgtta ctgcattcac agtattaaac tgcccggtag
     1981 cataaatcaa agtgtcattt agtgtaagag aatatacggt accatcgcaa cccggatccc
     2041 aggtttgaac cacccccgaa gttttgttta attgaactaa ccgcaaacgg gcttgcccac
     2101 caatggatgt aaacgaacct ccggcataaa gttttgagcc tgttaacgca agcgcataaa
     2161 cagtgctgtt tggattgggc ttaaatgctg catcaataac attgcccgaa gtaatgtgag
     2221 ccagattact tgcaccaaca gtagacgaaa aactgcctcc aatgtaccaa cctccattac
     2281 tgtcggtgat acaagtatat atggttccta ctacacccgg gaaagatgga tttgaaagac
     2341 ctgtggtaga agatattttt gcacaaccac gtttattttg tcctatacta ttaaagtatc
     2401 caccaaaata caactctttg ttgatagtat ctgcggctat ggaatataca gggccatcga
     2461 tagagttaat tgtaacattg gcccctgtac ttgcaattaa agcacctata cccgattgtt
     2521 gtgtagtacc aacggtagta aagcttccgc caaagtatag ggtatcacca attaatttta
     2581 aatcgtaagc agcattattt aaattgggat cggtaggtag ggctgcactt gtcgatttgt
     2641 tgatggcggc aatgtatctg cgtgttacac ccgacattat tgtaaatgct ccacagacat
     2701 acaagcggtt gcctaccaac ttcaaatcat atacggcacc atttgggttg ggagtaaatg
     2761 tggcattaat agctttcgaa gaatttacac gtataagatt tggttttcct ccggcagtaa
     2821 aacttcctcc tacataatat cctcctgcac catcggaaat aacaacattt acttcgccat
     2881 ttacttttat tg
//
LOCUS       DAORQA010000222         2885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8080_length_2885_cov_8.437102, whole genome shotgun sequence.
ACCESSION   DAORQA010000222 DAORQA010000000
VERSION     DAORQA010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8080_length_2885_cov_8.437102"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..1267)
                     /locus_tag="PK736_08325"
     CDS             complement(278..1267)
                     /locus_tag="PK736_08325"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRC33435.1"
                     /translation="MKVAVVGATGLVGTKILEVLAERNFPVTELLPVASAKSAGKKIT
                     FKNKEYDVITPEQAIEMKPALALFSAGGNTSLHYAPLFAAAGTTVVDNSSAWRMDPAK
                     KLVVPEVNADALEATDKIIANPNCSTIQMVVALKPLHDAYKIKRVVVSTYQSVTGTGK
                     AAVDQLMQERNNLPVTMVYPHTIDLNVIPQVDVFLDNDYTKEEMKMVLETQKIMGDNN
                     IKVTATTVRIPVMGGHSESVNVEFENEFEISKIKEILSKADGVILQDNPSEKIYPMPK
                     YAHESDAVFVGRVRRDETQPRTLNMWIVSDNLRKGAATNAVQIAEVLLKNGWL"
     gene            complement(1278..1796)
                     /locus_tag="PK736_08330"
     CDS             complement(1278..1796)
                     /locus_tag="PK736_08330"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRC33436.1"
                     /translation="MIRNKVFAKETLSIQTELVLPNDTNVLGNLMGGRLLHWMDIVAA
                     ISAHRHCHRVVVTASVNNVSFNQPIKLGQIVTLHGKVSRSFNSSMEVFIDVWVEDNAA
                     DKKIKCNEAIYTFVAVDQLGYPIAVPELIPETDEEKDRHQGALRRKQLSLILAGKMKP
                     ADATELKALFTI"
     assembly_gap    1964..1973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2179..2510
                     /locus_tag="PK736_08335"
                     /pseudo
     CDS             2179..2510
                     /locus_tag="PK736_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            2529..>2885
                     /locus_tag="PK736_08340"
     CDS             2529..>2885
                     /locus_tag="PK736_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6428 family protein"
                     /protein_id="HRC33437.1"
                     /translation="MKLSEFKSQLRNTESVTFSLPNGKDVPAHFHITEAGVVTKDFID
                     CGGTVRSEKFITMQLWTSIDFYHRLSSRKLLGILSKAEKLFASDDLEVEIEYQQETIG
                     RYGIEAGTNKFVLTSKH"
BASE COUNT          849 a          585 c          516 g          925 t
ORIGIN      
        1 catggttacc atatccacgg ccatcaaact caattctttc tactacccca tcgccttaca
       61 ctcctggtcg catgcccgaa tggattaaga aaaattagag gtttcgatat tgtgaacttc
      121 ttgcatcatt gattttattg taaatcggaa acaatattat aaagcagtta aatcaaaaat
      181 ataaaatctc tttgtaggtt tattaacttg ataaactata actgtatacg aagctacgcc
      241 ttataaaaag aaatcacgtt attggaataa caataaacta aagccagcca tttttcaaca
      301 acacttcggc tatttgcacc gcgttggtgg ctgcaccttt gcggaggtta tcgctcacta
      361 tccacatgtt taacgtgcga ggttgagttt catcgcgcct tacgcgtcct acaaaaacgg
      421 catcgctctc gtgtgcatac ttgggcatgg ggtatatttt ttcggatgga ttgtcttgca
      481 gtattacccc atcggcttta gataatattt cctttatttt actaatctca aattcgtttt
      541 caaattccac atttacggat tcgctgtggc cccccattac agggatacgc actgttgtag
      601 cagtaacttt aatattatta tcgcccatta ttttttgggt ttcgagcacc attttcattt
      661 cttctttggt gtaatcgtta tctaaaaaaa catcaacctg tggtatcaca ttcaaatcga
      721 tggtatgcgg atataccatt gttaccggca agttattgcg ctcttgcatt aattgatcta
      781 cagcggcctt tcctgttccc gttacacttt ggtatgtaga cacaacaaca cgttttattt
      841 tgtacgcatc gtgcaaaggc ttaagtgcca ccaccatttg tatggtactg caattaggat
      901 tggcaattat tttatcggtg gcttccagag cgtctgcatt cacttcgggc actaccagtt
      961 tttttgcagg gtccatacgc catgccgatg aattgtcgac aactgtggtg cccgctgctg
     1021 caaacaaagg tgcatagtgc aacgatgtat tgcccccggc cgaaaacaat gccaaagcag
     1081 gcttcatttc aattgcctgt tcgggagtga taacatcata ctctttattc ttaaaagtaa
     1141 ttttctttcc tgccgatttt gctgatgcta cgggcaacaa ttcggtcacc ggaaaattgc
     1201 gttcggcaag tacttccaaa atttttgtgc ccaccagtcc ggtagcacct actactgcaa
     1261 ctttcatttt tgttttatta aattgtaaat aatgctttca attcggtcgc atcggcaggt
     1321 ttcatttttc ctgccagaat aaggctcaat tgcttgcgtc tgagtgcccc ttgatggcgg
     1381 tccttttctt catcggtttc gggtatcagt tcgggcacgg caataggata acccagttga
     1441 tcaacggcca caaatgtata aatggcttcg ttgcatttta tttttttgtc ggcagcgttg
     1501 tcttctaccc acacatcaat aaatacttcc atgcttgagt tgaacgaccg cgaaacctta
     1561 ccatgcagag ttactatctg tccaagtttt attggttggt taaacgatac attgtttaca
     1621 ctcgcagtta ccactacacg atggcaatgc ctgtgtgcac ttatggcggc tactatatcc
     1681 atccaatgca gcaatcgccc acccatcaaa tttcccaaca cgttagtatc attgggcaat
     1741 accaattctg tttgaatgct caatgtttct tttgcaaaaa ctttatttcg aatcatacaa
     1801 tatttttgcg caaaggtaac gcagccaatt tcattgacaa cttaattttg tatttaaaaa
     1861 ttgggtaggc cgtttttcga atttgaagaa tatttgagca ataatgtcaa atacatatcg
     1921 atgtaaagtt gtaacgaatc gggttaatgc atagtttttt tagnnnnnnn nnntacaacc
     1981 agaagttata tgtattcgtc caccaagtca gaaataagtt aaattaaaaa gtggttttgg
     2041 gaatgttttc gttttcaaat agtgagacat ttttttcaat gtttaatggc agtaacaaaa
     2101 tgaaattatt gttaaattct caatccaatg ttttttgtat taatatctat cgtaatattg
     2161 caatattata atatacaaat gggcgccact aaaaccgatc actttaccga caaacaaaat
     2221 gctattgcct ctattgctaa agcgatggga catccggcac gtattgctat catcgattac
     2281 ttactcaaaa ataattcatg tatctgtgtg atatagtgga ttccttgccg cttgctcagc
     2341 ctactgtatc gcaacatttg aaagaactaa agcatgccgg cataataaaa ggcaatgtag
     2401 aaggcactgc tatctgttat tgtatcgatg agaaatttat acaaaaactg catacctatt
     2461 gccaaacaat atttactaaa ctaaatgaga agaaacataa ttgttgttaa acattaataa
     2521 ataaacatat gaaattatca gaatttaaaa gtcaattaag aaataccgaa agtgtaacgt
     2581 tttctttgcc aaacggaaaa gatgttccgg cacatttcca cattaccgaa gccggtgtgg
     2641 taacaaaaga ttttatcgac tgcggaggaa cggtacgcag cgaaaaattt attaccatgc
     2701 aattatggac ctctatcgat ttctatcatc gcctttcatc aaggaaatta ttgggcattc
     2761 tatccaaagc cgaaaaatta tttgcatccg atgatttgga agtagaaata gaatatcagc
     2821 aagaaaccat tggtcgctat ggaatagaag ctggcaccaa taaatttgta ttgacatcta
     2881 aacat
//
LOCUS       DAORQA010000223         2880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8097_length_2880_cov_15.241062, whole genome shotgun sequence.
ACCESSION   DAORQA010000223 DAORQA010000000
VERSION     DAORQA010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8097_length_2880_cov_15.241062"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..323)
                     /locus_tag="PK736_08345"
     CDS             complement(30..323)
                     /locus_tag="PK736_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33438.1"
                     /translation="MDHKNIISFEGKLQYQILLVFLIAFHSYFGIYKGINNVKSDFPN
                     YYVSAHLLQTGDLAVAYNDSLFQIEAQKKDSKIHARFSLYPPTNALLVVASLF"
     gene            complement(326..1744)
                     /locus_tag="PK736_08350"
     CDS             complement(326..1744)
                     /locus_tag="PK736_08350"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRC33439.1"
                     /translation="MLIAVVYTRMHSLTLLVWLLIAGSFFTLAYIASYTTLLSMYIVT
                     VVSMLFSVEMQIPNTAAILRLPSEPLLIIMAIAFFLKQLRVYSFSSNEFNKADNIFIL
                     LFILVMCITCYTSSMLSVSIKASLVKILYLFNFYFVVKYFVALDKTIITKWFNLISVL
                     SVPLIIYYLWKHSSYDFSKGAAGDIVKPLFNDHTIYSCFLAFLAPYNFLKTLFTPITE
                     KPFTKLFHGFLFLLIMIAITASGCRAAWLSMIAVLIAGIIFKFKINYKWIIASVVAVS
                     IYVAFNFDNYVAESKRNRHDSSAANADITTQAQSVTNISSDNSNLERLNRWSCAWRMF
                     LDKPLTGFGPGTYQFQYLDYQLKEEITPISVTSPYNIRQGHGGTAHQEYLLVLSEMGI
                     FGFLFFIWLIILTWKRACNMLTSKINTEEKIIIAAMFFAMLTYVVHAFFNNFLDTDKT
                     AFLFLGSMAFINQIYASKKIVL"
     gene            complement(1816..2790)
                     /locus_tag="PK736_08355"
     CDS             complement(1816..2790)
                     /locus_tag="PK736_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33440.1"
                     /translation="MINDESKHSEMTNDTNLVWQLLVKKRKFVLLTTLLITITVYVFT
                     SPWVIKPKYKSEIIVYPPGTNSAKIFIERDPRFGADKEIDEHIQIFESVKLRDSIIHK
                     YDLVTHYNIDTAGPTKAYQLHAEYNDNIKIDRTRYNSIMVTVYDTDPVLAAAIANDII
                     TIGDKVKNEIIHTNLMQIFYSIEREFKLKSNALDEMALKVNAATKSTIAEGAKIATLN
                     TTDIIRQQANIQSYIKVYTEQRKNDLARLLQQYLNLMESYFLLQQNYLQAFTNANSDV
                     PSCYIISPAEVSYKKAFPPRLLLTLAGGAFGFFTAWFIVIVINKFNQP"
BASE COUNT          956 a          475 c          540 g          909 t
ORIGIN      
        1 tcaacgcgat ccattattgc aaacagcttt taaaatagag aagcaacaac aagaagggca
       61 ttggtaggag gatacaaaga aaatcgggca tgaatctttg aatctttttt ttgtgcttcg
      121 atttggaaaa ggctatcgtt gtaagccaca gctaaatcgc ccgtttgtag caagtgtgcc
      181 gaaacatagt aatttgggaa atcgcttttt acattattga tgcctttgta aattccaaaa
      241 tatgaatgga atgcaattaa aaagactaac aatatttgat attgtaattt gccctcaaac
      301 gaaataatgt ttttatgatc catagctaca aaacaatttt ttttgaagca taaatctgat
      361 tgataaaagc catagaccct aaaaacaaaa atgctgtttt gtcggtgtcc aaaaaattat
      421 taaaaaaagc atgaaccaca tatgtaagca ttgcaaagaa cattgctgca ataataattt
      481 tttcttcagt atttatttta gaggttaaca tattgcaagc acgtttccat gttaaaataa
      541 tgagccaaat aaaaaataga aatccgaaaa tacccatttc ggaaagtacc aataaatatt
      601 cctgatgagc ggtgccacca tgaccctgtc gaatattata tggcgaagta acgcttatag
      661 gagtaatttc ttccttaagc tgatagtcta aatattgaaa ctgatatgtg ccggggccaa
      721 agccggttaa aggtttgtcg agaaacattc gccaagcaca actccagcga ttgaggcgtt
      781 ccagattgct attgtccgat gaaatgttgg ttacagattg tgcctgagtt gtgatgtcag
      841 catttgcagc acttgagtcg tgacgattgc gtttgctttc ggctacatag ttatcaaaat
      901 taaaggctac gtatatactt accgctacaa cagaagctat tatccatttg taattaattt
      961 tgaacttgaa aataatgccg gcaattaaca cagcaatcat actaagccat gcagcacggc
     1021 aacccgaagc agttatggct atcattatga gcaagaataa aaatccatgg aaaagtttcg
     1081 taaagggctt ttctgttatg ggtgtaaata gtgttttcaa aaaattgtat ggcgccaaaa
     1141 aagctaaaaa gcaactataa atcgtgtggt cgttgaataa aggttttaca atgtcgcctg
     1201 cagcgccctt cgagaaatca tatgatgaat gtttccataa ataataaatt attagtggaa
     1261 cacttaaaac agaaatcaga ttgaaccatt ttgtgatgat ggttttatcc agtgcaacaa
     1321 aatattttac tacaaaatag aaattgaaca ggtaaaggat ttttaccaaa gatgccttta
     1381 ttgaaacgga aagcattgat gaagtatagc aagtgataca catcaccaaa ataaaaagaa
     1441 ggataaaaat attatctgct ttattaaatt cattagaaga gaatgagtag acccgcaact
     1501 gtttcaaaaa gaaagcgatc gccataatta taagaagtgg ttcggatggt aagcgaagta
     1561 tagccgctgt atttggaatt tgcatttcga cagaaaatag catactgaca acggtgacaa
     1621 tatacattga caataatgtt gtataacttg caatataagc caaagtgaag aaactaccgg
     1681 ctatcaacaa ccaaacaagt aaagttaagc tatgcattct cgtataaaca acggctatca
     1741 acataagaaa agccagtaaa tattggacta tccacgctgt cttattttga ataatcaaat
     1801 gacccggcat gaatactaag gttgattaaa tttgttgata acaatgacaa taaaccatgc
     1861 tgtaaaaaag ccgaaagctc ctccagccag tgtcaatagc aaacgtggcg gaaatgcttt
     1921 tttgtatgat acttcggcag gagaaataat ataacacgat ggaacatcgc tgttggcatt
     1981 agtaaaggct tgcaggtaat tttgttgcaa caaaaaataa ctttccatta aattaagata
     2041 ttgctgcaat aatctcgcga ggtcattttt tcgttgctcg gtatatactt ttatataaga
     2101 ttgaatattc gcctgctgcc ttattatatc ggtggtgttg agtgtcgcaa tttttgctcc
     2161 ttcggcaatg gtgctttttg tagctgcatt tactttcaac gccatctcat ccagtgcatt
     2221 ggactttagt ttaaattcac gttcgataga ataaaatatc tgcataaggt tggtatgtat
     2281 aatttcgttt ttcaccttgt cgccaattgt aataatatca ttagcaatgg cggcagccaa
     2341 tacagggtct gtatcgtaaa ccgttaccat aattgaattg tatcgtgtgc gatctatttt
     2401 aatattgtcg ttatattcag catgcagttg gtatgctttt gtggggcctg ctgtatcaat
     2461 attgtaatgc gttacaaggt cgtatttatg aatgatagaa tcgcgcaatt ttaccgattc
     2521 gaaaatttga atgtgctcgt caatttcttt atcggcccca aatcgtgggt cgcgctcaat
     2581 aaatattttt gcgctgttgg tgccgggtgg gtaaacaata atttccgatt tgtattttgg
     2641 ttttattacc caaggcgaag taaatacgta tacagttatg gtaataagta atgttgtaag
     2701 caaaacaaat tttcgctttt tcaccaacag ttgccaaacc aaatttgtat catttgtcat
     2761 ttctgaatgt ttgctttcat cgtttatcat aattaaaatc cttctttttc tggttgacgg
     2821 tatttaaata atttttgtaa ggctttaatt tcgtatggct tgctatacaa ttttttgttg
//
LOCUS       DAORQA010000224         2879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8101_length_2879_cov_7.978045, whole genome shotgun sequence.
ACCESSION   DAORQA010000224 DAORQA010000000
VERSION     DAORQA010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8101_length_2879_cov_7.978045"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2108
                     /gene="mutS"
                     /locus_tag="PK736_08360"
     CDS             <1..2108
                     /gene="mutS"
                     /locus_tag="PK736_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRC33441.1"
                     /translation="HFNPSEIIFQKRKSKEFFDRYGKKYFTFQLEEWIFQEAYAVELL
                     TKQFQSKSIKGFGIEQMPAAIIAAGVAMHYLNETQQKALNHISTISRIEEDKYVWLDK
                     FTVRNLELIASPHDKAHKFIDVIDKTVSPMGGRLLRRWVLLPLKEINDIKQRQDVVSC
                     FVNNQTPSQEIISLLKSIGDLERLVSKAAMGKLGPREAVQLRKSLQSIVPIKNLCAQL
                     NNETLSRILSQLDACESLTILLEKTLLTDPSALITKGGVIAEGVHAELDELRTISKGG
                     KDFLLEIQRRESELTQIPSLKVAFNNVFGYYIEVTNVHKSKVPPEWMRKQTLTNAERY
                     ITEELKVYEEKILGAEEKILTFEEKIYAELLQSIVPHITAIQRNAYILAQLDCLLSMS
                     QVAIQNNYVKPLIDDSFVLDIKAGRHPVLELQMNPGEEYIANDLYLDNEQQQIIMITG
                     PNMSGKSALLRQTALIVLLAQMGSYVPARQAHIGVVDKIFTRVGASDNISSGESTFMV
                     EMNETASILNNISARSLILLDEIGRGTATYDGISIAWAIAEFIHEYSKTKAKTLFATH
                     YHELNEMTTSFARIKNFNVSIKETNNSILFLRKLVPGGIEHSFGIHVAKMAGMPASVI
                     TRAQELLAQLEKAHSSTEMVKQIQHKEKNDAYQLSFFQLDDPVLQQIREQLLSIDVNT
                     LTPVEALMKLNEIKKMLGR"
     gene            complement(2112..>2879)
                     /locus_tag="PK736_08365"
     CDS             complement(2112..>2879)
                     /locus_tag="PK736_08365"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HRC33442.1"
                     /translation="FYNLSAFISHLLNFIIMYKFFPIAILLFIVANLAYAQPAVYAKN
                     DSIKTPIEGLKNGVLVEIKNESNETSEIGILIDGKKDGAWKKYFLNGPMQRLEEYRKG
                     VLFGSVIELNRGGTLELDENYVNGKLNGRRIKYHYGMQSIVENYKMGILDGSRTTYYE
                     NNQKQEESFYINAKRDGAVKWYNQEGKLITEFTYKNGRLDGPAKNLFPDGKLQSEGMH
                     KNDVEEGEWKEYDEQGTLIKTTIYKAGVVVKEIPAKK"
BASE COUNT          928 a          555 c          549 g          847 t
ORIGIN      
        1 aacattttaa tcccagcgaa atcatttttc aaaaacgtaa atcgaaagaa tttttcgacc
       61 gctacggaaa aaaatatttc accttccaat tagaagaatg gatttttcag gaagcatatg
      121 cagtagaatt gctcaccaaa caatttcaaa gtaaatcgat aaaaggattt ggaatagaac
      181 aaatgccggc agcaattatt gcggctggcg tagctatgca ttaccttaac gaaacacaac
      241 aaaaagcact caatcatatt tccaccattt cacgaataga agaagataag tatgtatggc
      301 tcgataaatt tacggtgcgc aacctggagt tgattgccag cccgcacgat aaagcgcaca
      361 agtttatcga tgtgatagat aagaccgtgt cgcctatggg tgggaggctg cttcgcagat
      421 gggtattgtt gcctttaaag gaaataaatg atataaaaca aaggcaagat gtagtttcat
      481 gttttgtaaa taaccaaacc ccatcacagg aaattatttc gttattaaaa agcattggcg
      541 atctggaacg tctggtatca aaggctgcca tgggcaaact tggcccgcgc gaagcagtgc
      601 aattgcgcaa atcactgcaa agcattgtgc caataaaaaa cctatgtgct caactaaaca
      661 acgaaacgct tagtcgtata ctgtcacagt tagatgcttg cgagagtctc accattttat
      721 tggaaaaaac attacttacc gatccctcgg ccctaattac caaaggtgga gtaatagctg
      781 aaggcgttca tgctgagttg gatgagttgc gaactatatc gaaagggggc aaggactttt
      841 tactcgaaat acaaaggcgc gaaagtgaac ttacacaaat tccatctcta aaagtagcat
      901 tcaacaatgt atttggatat tacattgaag taaccaatgt gcataaaagc aaagtgccac
      961 ccgagtggat gcgcaagcaa actcttacca atgccgagcg ctacatcaca gaagagttga
     1021 aagtatatga agaaaaaatt cttggtgctg aagaaaagat tctcacgttc gaagaaaaaa
     1081 tttatgctga attattacag tcaattgttc ctcacataac tgccatacaa cgcaatgcct
     1141 atatattggc acagctcgat tgtttgctca gcatgtcgca ggtggctatt caaaacaatt
     1201 atgtaaagcc acttatcgat gattcgtttg tgcttgatat aaaagccgga cgccatcctg
     1261 tactcgaatt gcaaatgaat cccggtgaag aatatattgc caatgattta tatcttgata
     1321 atgaacaaca gcaaataatt atgattaccg gccccaacat gtctggaaaa tcggcattgc
     1381 tgcggcaaac agctttgatt gttttacttg ctcaaatggg tagttacgta ccggcaagac
     1441 aagcacatat tggtgtagtc gataaaattt ttacccgcgt aggtgcaagc gataacatat
     1501 cgagtggcga atcgaccttt atggtggaga tgaatgaaac agcaagtatt cttaataata
     1561 taagtgcgcg cagtttaatt ttgcttgacg aaattgggcg tggtacagca acatacgatg
     1621 gcatatctat tgcgtgggct attgcagaat ttattcatga atattcaaag accaaagcaa
     1681 aaactttatt tgcaacacac taccatgagt tgaacgaaat gacaacatcg tttgcacgca
     1741 tcaaaaattt taatgtaagt attaaagaaa ccaacaacag cattttgttt ttacgcaaac
     1801 tggtgccggg tggaatagaa catagttttg gaatacatgt tgcaaaaatg gcgggcatgc
     1861 ctgcttcggt aattacgcgt gcacaggagt tgcttgcaca attagaaaaa gcccacagca
     1921 gcaccgaaat ggtaaagcaa attcaacaca aagaaaaaaa cgatgcttat cagttaagtt
     1981 tttttcaact tgacgatccg gttttgcaac aaatacgcga acaattatta agcatagatg
     2041 taaacaccct tacccctgtt gaagcattga tgaaattgaa tgagataaaa aaaatgcttg
     2101 ggcgttagcg attatttttt tgcgggaatt tcttttacaa ctactcctgc tttgtaaata
     2161 gttgttttta ttaaagtgcc ctgctcatcg tattctttcc attccccttc ttccacatca
     2221 tttttatgca tgccttcgct ttgtaatttg ccatcgggaa ataaattttt tgccgggcca
     2281 tcgagccttc catttttgta agtgaattca gtaattaatt tgccttcctg attgtaccat
     2341 tttactgcac cgtcacgttt agcattgatg taaaaactct cttcctgctt ttgattgttt
     2401 tcataataag ttgtgcggga accatcgaga attcccattt tataattctc cacaatgctt
     2461 tgcattccat aatggtattt tattctgcga ccgttcaatt ttccattcac ataattttcg
     2521 tcaagctcga gtgtacctcc gcggttgagt tctattacag aaccaaaaag aacacctttg
     2581 cgatattctt ctaaacgttg catagggccg ttgaggaaat attttttcca tgcaccatct
     2641 tttttgccgt caatcaaaat ccctatttca ctggtttcgt tgctttcatt ttttatttca
     2701 accagcactc cgttttttaa tccttcaatc ggtgttttaa tagaatcgtt tttagcatac
     2761 acagcaggtt gcgcatatgc aagatttgca actataaata aaagaatagc gataggaaaa
     2821 aatttgtaca taattataaa gtttaataag tgcgaaataa acgctgataa gttatagaa
//
LOCUS       DAORQA010000225         2868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8141_length_2868_cov_6.775329, whole genome shotgun sequence.
ACCESSION   DAORQA010000225 DAORQA010000000
VERSION     DAORQA010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8141_length_2868_cov_6.775329"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1391
                     /locus_tag="PK736_08370"
     CDS             3..1391
                     /locus_tag="PK736_08370"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HRC33443.1"
                     /translation="MGIGPSSSHTLGPWRAAERFIFDLKSKTSLHNIQRIQVWLYGSL
                     AKTGIGHGTDVAVLLGLMGADPVTFDTTLVNDTIEKIKRNKTLSILDEIKVPFYYEKD
                     LVFLKQESLPFHPNAMKIVATLKDTQTEEAIYYSIGGGFVVKDGEDNSTHKTVVLKYD
                     VSNADELMQNCMRAGLNISALVLQNEQAWRTEEKTLQGILQIWNTMSECMYKGCHTVG
                     LLPGGLHVTRRAATINKKLLSASEYANIEEWKQAIKKSPHDFSSILNWVSCFALAINE
                     ENASFGRVVTAPTNGAAGVVPAVLMYFHLFAGGDADEMIIRFLLTASEIGSMFKKGST
                     ISAAMGGCQAEIGVSSAMAAGALTECLGGNPRQVMQAAEIAMEHHLGMTCDPIGGLVQ
                     IPCIERNTMGAIKAITASQLAMQSDPDAAKVSLDAVIKTMWETALDMNSKYKETADGG
                     LAIHIPLGLSEC"
     gene            complement(1458..2510)
                     /gene="queA"
                     /locus_tag="PK736_08375"
     CDS             complement(1458..2510)
                     /gene="queA"
                     /locus_tag="PK736_08375"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRC33444.1"
                     /translation="MKLSQFKFNFNENLLATHPAKNRDESRLMVVNRTTGKIEHKVFK
                     DILEYFDDKDVMILNNTRVFPARLYGNKEKTGAKIEVFLLRELSKDSRLWDVLVDPAR
                     KIRIGNKLYFGDNDLLVAEVIDNTTSRGRTLRFLFDGTHEEFKAAVYNLGHTPLPKYI
                     KRKAEAEDVHRYQTVYAKNEGAVAAPTAGLHFSRELLKRLEIKGVNFAEVTLHVGLGT
                     FRSVEVEDLTKHKMDSEQFEVNAEACDIVNAAKAAKRRICAVGTTTMRAIESTVSTNG
                     ELKPLAGWTNKFIFPPYDFSIANCMVTNFHMPESTLLMMTCAFGGFENIMNAYKVALK
                     EKYKFYSYGDAMLIID"
BASE COUNT          857 a          588 c          606 g          817 t
ORIGIN      
        1 aaattgggat aggcccatcg agctcccata ccctcgggcc atggcgcgct gccgaaagat
       61 ttattttcga tttaaaatcc aaaacctcat tacacaacat acaacgtata caagtatggt
      121 tgtatggctc gctggcaaaa accggtatag gccatggcac tgatgttgct gtcctgcttg
      181 gtttgatggg tgccgacccc gttacatttg acacaacatt ggtaaacgac accatcgaaa
      241 aaattaaacg caacaaaaca ctttcaattc ttgatgaaat aaaagtgccg ttttattatg
      301 aaaaagattt ggttttttta aaacaagaat cgttgccatt tcaccccaat gcaatgaaga
      361 tagtggcaac attgaaagat acacaaaccg aagaggctat ttattattcg ataggtggag
      421 gctttgttgt aaaagatggc gaagataatt ctacacataa aactgttgtt ctaaaatacg
      481 atgtaagtaa tgccgatgaa cttatgcaaa attgtatgcg ggcgggcctg aatatttctg
      541 cactggtact tcaaaacgaa caagcctggc gcacggaaga aaaaacccta cagggcattt
      601 tgcaaatttg gaacaccatg agcgagtgca tgtacaaagg ttgtcacacc gttggattgt
      661 tgcccggagg gttgcacgta acgcgaaggg ccgccacaat aaataaaaaa ctactgagtg
      721 ctagtgagta tgcaaatatt gaagagtgga agcaagctat aaaaaaatcg ccacacgatt
      781 ttagctccat tttgaattgg gtgagctgct ttgcgctagc tattaatgaa gagaatgcct
      841 cgtttggccg tgtagtaaca gcaccaacaa atggcgctgc gggagtggtg ccggctgtgt
      901 taatgtactt tcatttattt gccggtggcg atgccgatga aatgataatt cggtttttac
      961 tcactgcctc cgaaatagga agtatgttta aaaaaggctc taccatttct gcggcaatgg
     1021 gcggttgcca ggccgagatt ggcgtttcat cggcaatggc tgccggtgca ttaacggaat
     1081 gcttaggtgg aaatccgcgg caagtaatgc aagcggccga aattgcaatg gagcatcatt
     1141 tgggaatgac atgcgaccct attggtggtt tggtgcaaat tccttgtatt gaacgaaata
     1201 caatgggcgc cataaaagca attacagcat cgcaacttgc catgcaaagt gaccccgatg
     1261 ctgctaaagt ttcgctcgat gcggtaataa aaaccatgtg ggaaacggca ctcgatatga
     1321 atagcaaata caaagaaact gccgatggcg gactggccat tcatatccca ttaggattga
     1381 gcgagtgcta aaaaaaatgc cgctaaccac atcagattat gtagttggcg gcattcccat
     1441 ttcaaacaat acgatggtta gtctatgata agcatagcat caccataact ataaaatttg
     1501 tatttttctt tcaaagctac tttgtaagca ttcataatgt tttcgaaacc accaaatgcg
     1561 caagtcatca ttagcaaagt agactcgggc atgtggaaat ttgttaccat acaattggca
     1621 atactaaaat cgtacggagg aaaaataaat ttatttgtcc agccggctaa cggtttcaat
     1681 tcaccatttg tagaaactgt agattctata gcacgcatag tggtagttcc caccgcacaa
     1741 attctgcgct ttgcggcttt ggcagcgttc actatatcac aggcttcggc atttacttca
     1801 aattgttcgc tatccatttt atgtttggta aggtcttcaa cttctaccga acggaaagtg
     1861 cccaaaccta cgtgaagcgt aacttcggca aagtttaccc ctttaatttc gaggcgctta
     1921 agcaattcgc ggctaaagtg taaccctgcc gttggcgctg ccacagcacc ttcgtttttg
     1981 gcatacaccg tttggtagcg atgcacatct tcggcttcgg ccttacgttt tatgtatttg
     2041 ggaagaggtg tatgaccaag attataaacg gcagctttaa actcctcgtg tgtaccatcg
     2101 aaaaggaaac gaagtgtacg cccacgcgat gtggtgttgt ctattacttc ggccaccaat
     2161 aaatcgttat cgccaaaata tagtttattg cctatgcgga ttttacgtgc agggtctact
     2221 aatacgtccc aaagacgact atccttgctc aactcgcgca acaaaaacac ttctattttg
     2281 gcccctgttt tttctttgtt accatacaat cgtgcaggaa atacgcgggt gttattcaat
     2341 atcattacat ccttatcgtc aaaatattcg agtatgtctt taaacacttt gtgttctatt
     2401 ttgccggtgg tacggtttac caccatcaga cgcgattcgt cacgattttt ggccgggtga
     2461 gtagccagga ggttttcatt gaaattgaat ttaaactgcg agagtttcat tttacactta
     2521 catttatggg caacatgcct tgttttaaag caaattgcta ttgttaatat tatccttcat
     2581 tcggctgcca acacattgat tatcaggggg aaaaccaata aaatacgcaa ccttttttta
     2641 taaacggggc gcaaatataa tatcatgata tactgttttc gacatggagg gcaaaataaa
     2701 tattaccaat ttataattga tctctaaagc gttgcattaa taagtgttac agagatatct
     2761 atgctatcaa aataattaag cccaagttgc aaatcatatt tttttaggat cttctttttt
     2821 aagaaaatac gctgcaacag cagaaattat taaaccccct gcaagcac
//
LOCUS       DAORQA010000226         2852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8191_length_2852_cov_7.537004, whole genome shotgun sequence.
ACCESSION   DAORQA010000226 DAORQA010000000
VERSION     DAORQA010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8191_length_2852_cov_7.537004"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="PK736_08380"
     CDS             <1..1531
                     /locus_tag="PK736_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33445.1"
                     /translation="SRNDQAQKIITDDVNPLITYVVGLTNIGNGDVAVHVRKYDQFGN
                     LMASVSGPQFGAPSPNGGQVKKLLWGNDGNLYVFYNQDDSILPQSAKIVFKFNKSLNL
                     IKQLYLNANMTDAVKISSNDFISVSNNYNGINYLVYARINSNLNLVYNFWWDYDNNDL
                     TKYDESPAQMVYSNGYIYVAGTIRNTLNNRTARFVQKFDTNMTSSNPIIWTYVSSFAT
                     KKRSYSTLSVIGNFVYCAGKDADSVSFIAKHNLSTGAVVNSRTLKTAGAQNEYVKILN
                     DNTGGVKVVGYSRNNSLSFYKVNTVKYSADLVTQNYNQSYTLSYNSKIADVINGSYFP
                     GSSYITGYTTLGGRRKLMILTHNAPNYSIDTLFAANSEGLAITTNYDPSFSLERPVSC
                     GYYNPVTSIFLSADNQFVARGYYGTINRMAATNIDNFGVRTYPNPSAQYLNFEFENPL
                     NESVRIFITNALGVLVLGPIGVSSSNVPDPINIATLSAGTYFYSIQANNLNLRGSFVK
                     Q"
     gene            complement(1726..>2852)
                     /gene="odhB"
                     /locus_tag="PK736_08385"
     CDS             complement(1726..>2852)
                     /gene="odhB"
                     /locus_tag="PK736_08385"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HRC33446.1"
                     /translation="VEKDEEIAEIDSDKATLSLVAEASGKLTVTVNEGESVAVGSVVA
                     TIDETVAAPATKEKTTTAEPKKEVVAEKKMEAVVQEKTASPAAKKLMADNAITTEQLT
                     ATGKGGRITKQDVLAASQNGNGLKTSAAAPRESKREKMTTLRKKLSQRLVAVKNQTAM
                     LTTFNEIDMSTVMAIRTKYKDTFKEQHGVGLGFMSFFTKAVCEALQAFPMVNAYIDND
                     EIELHNYCDVAIAVSAPKGLMVPVIRDAQNLSLAQIEKEILALATKARDGKLTIDEMT
                     GGTFTITNGGVFGSLMSTPILNPPQSAILGMHNIVERPVAVNGAVVIRPMMYVALSYD
                     HRIIDGKESVGFLVKIKQMIEDPVKMFFAGEDPVKKLLQL"
BASE COUNT          853 a          541 c          519 g          939 t
ORIGIN      
        1 tagtaggaat gatcaagctc aaaaaataat aaccgatgat gttaatccat tgataaccta
       61 tgtagttgga ctaacaaata taggaaatgg agacgtggcg gtgcatgtgc gcaaatacga
      121 tcagtttggc aatcttatgg caagtgtcag cggcccccaa tttggagctc catcaccaaa
      181 tggtgggcaa gtaaaaaaat tactttgggg taacgatggc aatttgtatg tattttataa
      241 tcaagacgat agtattttac cgcagtcagc taagattgtt tttaaattca acaaatcatt
      301 aaatttgata aaacaattat atttaaatgc taatatgact gatgctgtta aaatttcaag
      361 caatgatttt atttcggttt caaataatta taatggcata aactatctgg tatatgcacg
      421 cataaattct aatttaaatt tagtatataa tttctggtgg gattacgata ataatgattt
      481 gacgaaatat gacgaaagcc ctgcacaaat ggtatatagt aatggttata tatatgtagc
      541 cggcactatt cgcaatactt taaataatag aacggcaaga tttgtgcaaa aatttgatac
      601 aaatatgacc tccagcaatc ccataatttg gacatacgtt tcaagctttg ctacaaaaaa
      661 aagaagctat agtacactgt cagttatagg caactttgtt tactgtgccg gaaaggatgc
      721 tgatagtgta tcttttattg caaagcataa tttgagcaca ggtgctgttg ttaattcgcg
      781 aactttaaaa actgctggtg cacaaaacga atatgtgaaa atattaaatg ataataccgg
      841 tggcgtaaaa gtagttgggt acagtcgcaa taacagcttg tctttttata aagttaatac
      901 tgtaaaatat agtgccgatt tagtaaccca aaattataat caatcatata cactaagtta
      961 taattcaaaa attgccgatg ttataaacgg aagttatttt cccggcagtt catatattac
     1021 tggctacaca actttaggtg gacgtagaaa attaatgatt cttacacaca acgcaccaaa
     1081 ctattctatc gacactttat ttgccgccaa ttcagaaggc ctggcaataa ccacaaacta
     1141 tgatccatca ttttcgcttg aacggcctgt ttcttgtggc tattataatc ctgttacatc
     1201 aatattttta tcggccgata atcaatttgt tgcccgaggg tattatggaa ctataaatcg
     1261 aatggctgcc actaacattg acaattttgg tgtacgcact taccctaatc caagcgcgca
     1321 atatctcaac ttcgagttcg aaaatccatt aaacgaaagt gtgagaatat ttattaccaa
     1381 tgccttagga gtattggtac taggacctat tggtgtttca agtagtaatg ttccagaccc
     1441 aattaatatt gccacattga gcgcaggaac ttatttttat agcatacaag ccaataatct
     1501 aaacttacga ggatcgtttg tgaagcagta attaggcgca aactttacaa tcaatgtacc
     1561 aatactcaaa taaaaataaa aaccgatact tgattgtttt ttttgcaggt aaaaaaatcg
     1621 agtattagct tatctatcgt atatggttat tgttactaaa aaatgaatct tctaaattct
     1681 ccgtttgtaa atcataatta ctttcggaag taaaatgcca aatagttata attgcaataa
     1741 tttttttaca ggatcttctc cggcaaaaaa catcttaaca ggatcttcta tcatctgttt
     1801 tatttttaca agaaatccca cactttcttt gccatcaatg atgcggtgat cgtagcttag
     1861 agcaacgtac atcataggac gtataactac agcgccattt actgcaacag ggcgttctac
     1921 aatattgtgc attccaagta tggcagattg cggagggttc aatataggtg ttgacatgag
     1981 gctgccaaac acaccaccgt ttgtaatggt aaatgtgcct ccggtcattt catctattgt
     2041 caatttccca tcgcgtgctt tggttgcaag ggcaagaatt tctttttcaa tttgtgccaa
     2101 tgataaattt tgtgcatcgc gaattacagg caccatcaac cctttgggcg cacttacggc
     2161 aatggcaaca tcgcagtaat tatgtaattc tatttcgtca ttatcgatat aggcattcac
     2221 cataggaaat gcttgcaatg cctcacacac cgcttttgta aaaaacgaca taaaccctaa
     2281 acctacacca tgctgttctt taaaagtatc cttgtatttt gtacgtattg ccatcaccgt
     2341 gctcatatca atctcgttga aagtagttag catagcagtt tgatttttaa ctgctaccaa
     2401 ccgttgcgat aattttttgc gcagggtagt catcttttcg cgtttgcttt cgcggggtgc
     2461 tgcggcactg gtttttaagc cattaccgtt ttgcgatgct gcaagtacat cttgctttgt
     2521 tatgcgccct cctttgccag tggctgtcaa ttgttcggtg gtgatggcat tatcggccat
     2581 gagttttttt gctgccggtg atgctgtttt ttcctgaacc actgcttcca ttttcttttc
     2641 ggcaactact tcttttttag gctcggctgt tgttgttttt tctttggtgg caggtgctgc
     2701 tacagtttca tctatggttg caactacact tcctactgca acactttcgc cttcgtttac
     2761 agttaccgtt aactttccgc ttgcttcggc cactagcgat agtgtagctt tgtcagaatc
     2821 tatttcggca atctcttcgt ccttttccac at
//
LOCUS       DAORQA010000227         2831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8267_length_2831_cov_7.253242, whole genome shotgun sequence.
ACCESSION   DAORQA010000227 DAORQA010000000
VERSION     DAORQA010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8267_length_2831_cov_7.253242"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(499..>2831)
                     /locus_tag="PK736_08390"
     CDS             complement(499..>2831)
                     /locus_tag="PK736_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33447.1"
                     /translation="MTGDKTDYCNGSDDYWVVKIDSIGNILWQNTIGGGNDDMLLSMV
                     QSQNGGYVLGGYSTSDISGDKTKNSHGGMDYWLLQLDSLGNIVWQNTIGGDANDLPYS
                     ITPSNDGGYILGGTSTSNISGDKTENSNGLSDYWVVKIDSIGNIVWQNTIGGIGTDML
                     LSITNSLDGGYILGGHSWSSISGDKSENCIGSEDYWVVKIDSVGNILWQNTIGGSESD
                     RFAFVSHAPDAGFILGGWSFSNISGDKTENKKGVCDYWIVKIDSLGNIDWQNTIGGSS
                     IEKLSCATLSLDGGIVLSGNSISNISGDKEENAIGSYDFWILKLSHSSRITGKLFADI
                     NQNNIQDNGEANMIGLKITEVNTGRSTFSQSDGKYAIGVHDTGVFVLNPSTQFYYASN
                     PPTHTATFTAIQQIDSLNDFAFQPTIVTNDLLISIHPTTCFRSNFTGMYNVHYKNIGT
                     TTLWPTIYFFNDSNIAFSTSSIAPNNVYADSIIWNLPPIVPYMEYDFTITVGVNIGLQ
                     NGTQINSSIKIFPFTNDTDITNNFASWVLQTIGSYDPNEIIVSRDSLDIMELNPVPPF
                     LDYVIYFQNTGNDTAFNVKVENIIPPQLELGSFEYLSSSSPVNISLNSQSRLASFEFP
                     NIQLPDSNIDEPGSHGYIRYRIQPLNTLQVNDSILSTAAIYFDFNAPVITNTAHTIIG
                     MNVLSAQSEIQLTNNFSVMPNPAKNNLTIAFKNQTQANEQLEIISATGKLLKKIKVQP
                     HTTKMEIDISKLPNGFYLLKYNQAERVESIKFVVER"
BASE COUNT          880 a          552 c          481 g          918 t
ORIGIN      
        1 attcactact acacaaactt acacctacat tggcaaaatg ctcgaattct tttttattaa
       61 cgggggcggt ggcccctcgt gtgcaaccgc gccttgcgcg gttgcaataa attcgcttcg
      121 cttgtggcaa tcatgcacaa tcgattttta agtcaatcgt aaaacccgca gttacaatat
      181 gctgcagttc ttatgaatca ttggggaaaa ggccttgttg tatagacatt aatgccatag
      241 tctccgctca attttacatt ccaaacccgc aaggttttta aaaccttgcg ggttttactg
      301 cgcgaaaaaa ttcagcgtaa gcaatagagc cgtaggctcg actcaatatt gtaaggatgg
      361 atttcaatcc atcacattaa agcgtgaagc gaattaaatc ctatagggtt tttaaaaccc
      421 gtcaggattt taaacatgtg caaacaaaat atcccattac caaatactat atactcaata
      481 cctaataccc aatactcatt acctctctac tacaaatttt atcgactcta ctctttccgc
      541 ttgattatac ttcaacaaat aaaatccatt tggcagttta ctaatgtcaa tttccatttt
      601 agttgtgtgg ggttgcactt ttattttttt caataatttt ccggtggcag aaataatttc
      661 caattgctcg tttgcttgtg tttgattttt gaatgcaatg gtcaaattat tttttgccgg
      721 attgggcatc acactaaaat tgtttgtaag ttgaatttca ctttgagcgc ttagtacatt
      781 catgccaatt attgtgtggg cagtgtttgt aattacaggt gcattgaaat caaaataaat
      841 agcagcagtg cttaaaattg aatcgttaac ctgtaaagta tttagtggct gaatacgata
      901 gcgaatataa ccatgactgc ccggttcatc aatattacta tcgggcaatt ggatatttgg
      961 aaactcaaaa gaagcaaggc ggctttggct attgaggcta atgtttacag gcgatgacga
     1021 tgataaatac tcaaacgaac caagttctaa ttgtggtgga ataatattct ctactttcac
     1081 attgaacgca gtatcgttgc ccgtgttttg aaagtagatt acataatcca aaaatggagg
     1141 cacaggattt aattccataa tatcgagcga gtcacgactc acaataattt cgttggggtc
     1201 gtatgaacct atagtttgca acacccaact ggcaaaatta tttgtaatgt cagtgtcgtt
     1261 tgtaaaagga aatattttta tacttgagtt tatttgtgtg ccattttgta aacctatatt
     1321 tacgcctacg gtaattgtaa aatcatattc catataagga acgattggtg gcaaattcca
     1381 aatgatagaa tcagcataaa cattatttgg agcaatactt gaagtagaaa atgcgatgtt
     1441 ggaatcatta aaaaaataaa tagttggcca taaagtagta gtgccaatat ttttgtaatg
     1501 cacattgtac atgcccgtaa agttgcttct aaaacaagtg gtagggtgaa tgctaattaa
     1561 caaatcgtta gtaactatag ttggctgaaa agcgaaatcg tttaaagaat caatttgttg
     1621 tatagctgta aatgttgctg tatgagttgg tggattgctg gcgtaataaa attgtgtcga
     1681 tgggttcaaa acaaagaccc ctgtatcgtg cacaccaatt gcatatttac catcggattg
     1741 tgaaaatgta cttcgcccag tatttacttc tgttattttt agtccaatca tatttgcctc
     1801 gccattatct tggatattat tctggtttat gtctgcaaat aattttccgg tgattcggct
     1861 tgaatgggag agttttaaaa tccaaaaatc ataacttccg atagcgtttt cttctttatc
     1921 gccagaaata ttagatatgg aattgccact caaaacaata cctccatcaa gtgaaagggt
     1981 agcgcaagat aatttttcta tgctactgcc tccaattgtg ttttgccaat ctatatttcc
     2041 aagcgaatca atttttacta tccaataatc gcaaactcct tttttgtttt ctgttttatc
     2101 gcctgaaata tttgaaaacg accatccgcc caaaatgaat ccagcatctg gagcatgtga
     2161 aacaaaagca aagcgatcgg attcgctccc gccaatggta ttttgccata aaatgttacc
     2221 aacgctatcg atttttacaa cccaataatc ttcagaacca atacaatttt ccgatttgtc
     2281 tccagaaata cttgaccagg aatgcccacc aagaatatag cctccatcta aggaatttgt
     2341 aatcgaaagt aacatatcag tacctattcc accaattgta ttttgccaaa caatattacc
     2401 aatgctatca atttttacta cccaatagtc ggaaagcccg ttactatttt ctgttttatc
     2461 tccggaaata tttgacgttg atgttcctcc caaaatgtac cctccatcat ttgaaggagt
     2521 aatggaatat ggcaaatcat ttgcatcacc gccaatggta ttttgccaaa cgatgttacc
     2581 aagactgtct aattgtaaaa gccaataatc catgccgcca tgactatttt ttgttttgtc
     2641 accagatata tcagaagtag aataccctcc caaaacataa cccccatttt gtgattgaac
     2701 catggataac aacatgtcat cattaccccc tccaatagtg ttttgccata aaatgttgcc
     2761 tatgctatca atttttacta cccaataatc atcactacca ttacaataat ctgttttatc
     2821 gccagtcata t
//
LOCUS       DAORQA010000228         2806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8353_length_2806_cov_5.356234, whole genome shotgun sequence.
ACCESSION   DAORQA010000228 DAORQA010000000
VERSION     DAORQA010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8353_length_2806_cov_5.356234"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..532)
                     /gene="ssb"
                     /locus_tag="PK736_08395"
     CDS             complement(107..532)
                     /gene="ssb"
                     /locus_tag="PK736_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRC33448.1"
                     /translation="MSGLNKVMLIGNIGKDPEIRHLEGGNMMAKFPLATNEIYKTKDG
                     LRREITEWHNIVVWQRLAETIEKLQLKKGQLLYVEGRIRSRTWDDKDGHKHYATEIVA
                     DGMTILGRKITDGQPDDHHITNDSDPINGIQAEVGDLPF"
     gene            959..>2806
                     /gene="metG"
                     /locus_tag="PK736_08400"
     CDS             959..>2806
                     /gene="metG"
                     /locus_tag="PK736_08400"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRC33449.1"
                     /translation="MTKEFKRTLVTAALPYANGPVHIGHLAGCYLPADIYVRFMRSMG
                     EDILFICGSDEHGVPITIKARNENTTPQLVVDKYNALMKVAFDGIGISFDHYSRTSSP
                     LHHQTASEFFTDLYNKGVFIEEVTKQYYDEEVNQFLADRYIEGTCPRCGNNRAYGDQC
                     ENCGTSLQPTDLIEPRSKLSGSKPVLKETRNWFLPLDKYQERIQQWLLTKQDTWKGNV
                     YGQCMSWINQGLQPRAMTRDLDWGVKVPLPDTDGKVLYVWFDAPIGYISATKEYFENT
                     EVQKKHGDATWKNYWCDESTRLVHFIGKDNIVFHCIIFPAMLMAHGEYVLPDNVPANE
                     FLNLEGDKISTSRNWAVWLHEYLLDFPNKQDVLRYALCASMPETKDNDFTWKEFQTRN
                     NSELVGILGNFVNRTLVLTHKFYDGLVPTRNQLLDIDKNVLETIAATPQRIQQSLTGY
                     KFREALMHVMDLARCGNKYLADTEPWKLIKTDAERVKTILNISLELTACLAAVAEPFM
                     PYTSATMFGMMNIDAVSFRELNKTLVAPGHKINEAKLLFEKIEDDAVQAQVDKLHATQ
                     TATASTTIEPQKAEVSYDDFTKMDIRIAEILEAERVPKTDKLLKLKLDTG"
BASE COUNT          821 a          554 c          633 g          798 t
ORIGIN      
        1 ggtaaggggt ccaaatctta attaatatta tttgttgctg gtaaaatcat gtgacaaaga
       61 aaggcaaaaa aaaaacaaaa tgaaaaaggg agtttacagc ccttatttaa aatggtaaat
      121 cgcccacttc ggcctgtata ccattgatag gatcactatc gtttgttatg tggtgatcat
      181 cgggttggcc atcggttatt ttgcggccca atattgtcat gccatcggct acaatctctg
      241 ttgcgtaatg tttatggccg tccttgtcgt cccaggtgcg gcttcgtatg cggccttcca
      301 catatagcag ctgtcccttt ttaagttgga gtttttcgat ggtttcggca aggcgttgcc
      361 ataccacaat gttgtgccat tcggtaattt cgcgccttag gccgtctttt gtcttatata
      421 tttcgttggt ggccaaagga aattttgcca tcatatttcc gccttcgagg tggcgaattt
      481 cgggatcctt gcctatattg cctataagca ttactttatt taatccagac atagcacaaa
      541 gttttttgac atgtgaaagg gaatgcaata gtagtaatat ttcgatcgcc ataaaaataa
      601 tgtggtgaat ggcgattttg tggtggcgta attttgtgtt acgatttaat tattaaagac
      661 ctacaatttg ttttctggtt aagggtcaac acatttttcc tcaacgtata tggtgattgt
      721 tgttgttttt attttgtaat aatgatatcc atttgttgag taatgcaaat gttgtaaagc
      781 ccttttacag aggcttaagc aggtggggca agatgcgtaa acgtattgta ccgggcgcaa
      841 ggcggccggt accggagagg ccaccgcctc ccgcaataaa aacagtattg atattactac
      901 ccttgcttgg gcaataactg caaccgatga ttatatttgc acactttgaa aaacggatat
      961 gacgaaagaa tttaagcgca ctcttgttac agctgcattg ccttatgcca atgggcccgt
     1021 gcatataggt caccttgcgg ggtgttattt accagccgat atttatgtac ggtttatgcg
     1081 cagcatgggt gaggatattt tgtttatctg tggcagcgat gaacatggtg tgcccattac
     1141 tataaaggcg cgtaacgaaa atactacacc tcaattggta gtagataaat ataatgcact
     1201 gatgaaagtg gcttttgatg gcattggcat ttcgttcgat cattactcgc gtacatcatc
     1261 gcccttgcat caccaaacgg catcggaatt ttttaccgat ttgtacaaca agggtgtgtt
     1321 tatagaagaa gttacaaaac aatattacga tgaggaggta aatcagtttt tggccgaccg
     1381 ctatatagaa ggcacctgcc cgcgttgtgg aaacaaccgt gcctatggcg atcaatgcga
     1441 aaattgcgga acatcgctac aacccaccga ccttattgaa ccacgaagca agctaagtgg
     1501 cagcaagcct gtgctcaaag aaacacgtaa ttggtttttg ccattggata aatatcagga
     1561 gcgtatacag caatggttac ttacaaaaca ggatacctgg aaagggaacg tgtatgggca
     1621 atgtatgagt tggataaatc agggattgca accacgtgcc atgacccgcg acctcgactg
     1681 gggtgtaaaa gtgccactac ccgacactga cggaaaggtg ctgtatgtgt ggttcgatgc
     1741 gcctatcggt tacataagcg ccaccaaaga atattttgaa aataccgaag tacaaaaaaa
     1801 gcatggcgat gccacatgga aaaattactg gtgcgatgaa tcgacacgcc tagtgcattt
     1861 tataggtaag gataatattg tttttcactg cattattttt cctgcaatgt tgatggcaca
     1921 tggcgaatat gttttgcccg ataatgttcc ggccaacgaa tttttgaatc ttgagggcga
     1981 taaaatttct acatcgcgca actgggcggt gtggctgcac gaatatttac tcgattttcc
     2041 taacaagcaa gatgtgctgc gttatgcttt gtgtgcatcg atgcctgaaa caaaggacaa
     2101 tgattttaca tggaaagaat ttcaaacccg caacaatagc gagctggtag gtatacttgg
     2161 caattttgta aaccgcaccc tggtacttac gcataaattt tacgatggct tggtgccaac
     2221 acgaaaccaa ttactcgata tagataaaaa tgtattggaa accattgccg ccactccaca
     2281 acgtatacaa caatcgttaa cgggttataa atttcgtgag gcattgatgc atgtgatgga
     2341 ccttgcccgt tgcggaaata aataccttgc cgataccgaa ccgtggaaac tgataaaaac
     2401 cgatgcagag cgcgtaaaga ctattttgaa tatttcgctc gaacttacag cttgccttgc
     2461 cgcagtggcc gaaccattta tgccttatac atcggctaca atgtttggta tgatgaatat
     2521 tgatgccgtt tcttttcgcg aattgaataa gacattggtg gcaccgggac ataaaataaa
     2581 cgaagccaaa ctgttgttcg aaaaaataga ggacgatgcc gtacaggcac aagttgataa
     2641 actgcatgcc acacaaaccg caactgcatc aactacaatt gaaccacaaa aagcggaggt
     2701 gagttatgac gattttacaa aaatggatat acgtattgcc gaaatactcg aggccgagcg
     2761 tgtacctaaa accgataaat tgctaaaact aaaattagat accggt
//
LOCUS       DAORQA010000229         2767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8472_length_2767_cov_5.558628, whole genome shotgun sequence.
ACCESSION   DAORQA010000229 DAORQA010000000
VERSION     DAORQA010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8472_length_2767_cov_5.558628"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1314)
                     /gene="gpmI"
                     /locus_tag="PK736_08405"
     CDS             complement(58..1314)
                     /gene="gpmI"
                     /locus_tag="PK736_08405"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRC33450.1"
                     /translation="MKKKVVLIIMDGWGEAPATHANAVSEAHTPFIDNCYKKYPHAQL
                     LTDGENVGLPAGQMGNSEVGHMNLGAGRIVYQDLVKLNKEVRDNLLKENVEWKKTVSY
                     CNENNKPLHLMGLVSNGGVHSHIEHLKHLCFLAHEAGIAKVFIHAFNYGRDTDPKSGL
                     AFIADLENYISQKNLKAKIATVCGRYYAMDRDKRWERIAIAYNAMVNSEGKKFSSATD
                     AIQSSYTDTVTDEFILPCIITEAGNAIAKIAEGDAVLCFNFRTDRCREITVALTQTDM
                     HEQNMHTMNLHYVTMTRYDESFNGVNVMYEKDNLNKTLGEVLSENGLHQLRMAETEKY
                     PHVTFFFNGGREAAFEKEDRIMEPSPKVATYDLQPEMSAVPLKNAFLKAIENKDYDFV
                     CLNFANPDMVGHTGVYDAVKKSRGNC"
     gene            complement(1326..2285)
                     /locus_tag="PK736_08410"
     CDS             complement(1326..2285)
                     /locus_tag="PK736_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRC33451.1"
                     /translation="MKRLQLLFIVLTTYILLQFGWWAYMLITLNNEVYENKVELVHLH
                     GNSNEALLKSDLEKKLRSKWWMIAGEGFVFIGLLSYGIYQTFLAFKKEYALAKMQKNF
                     LLSVTHEFKSPLAAVKLNIQTLAKHKLSAEQTKGIIESTLSETDRLNDLVENALTAAR
                     IDTHSFSLVYSQINLSNLVQSIVQMKQMTDNGYRKVKCNIQDNVMVLGDDMALSSVVL
                     NLIENAIKYSFDNVDVLISLQQQNENAILKIADKGIGMKQSEREKVFEKFFRIGNEET
                     RNTKGTGLGLYIVKNIVELHKGSISVTDNVPQGCVFTVTLPCK"
     gene            complement(2345..2417)
                     /locus_tag="PK736_08415"
     tRNA            complement(2345..2417)
                     /locus_tag="PK736_08415"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2382..2384),aa:Phe,seq:gaa)
     gene            complement(2575..>2767)
                     /locus_tag="PK736_08420"
     CDS             complement(2575..>2767)
                     /locus_tag="PK736_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33452.1"
                     /translation="YVTFHYAHSNRVIEELTSIPTCLCNKMQGHCCFLAVMQAGCFLL
                     STNIFVIDKVINSPSFERK"
BASE COUNT          805 a          575 c          481 g          906 t
ORIGIN      
        1 atgataaatg aataaccacg cgcaccggct tttgaaacca tttctgcaac gcaggcgtca
       61 acagtttcca cggctttttt ttacagcatc gtatacaccg gtatgtccta ccatatcggg
      121 gttagcaaaa ttcaaacaca caaagtcgta atccttattt tcaatagctt ttaagaaagc
      181 atttttcagc ggcacggcac tcatttcggg ttgcaaatcg taagtggcca cttttggtga
      241 aggttccata atacgatctt ctttttcaaa agcagcttcg cggccaccat taaaaaagaa
      301 agtaacatga ggatactttt cggtttcggc catccgcagt tgatgcaatc cgttttcact
      361 caatacttca cccagggttt tgttgaggtt atctttttca tacattacat tgacgccatt
      421 aaacgattca tcgtagcggg tcatggtaac ataatgcaaa ttcatagtat gcatattttg
      481 ctcatgcata tcggtttgtg taagtgctac agttatttcg cggcaacggt cggttctgaa
      541 attaaaacat aaaacagcat caccttcagc aattttagca atggcatttc ccgcttcagt
      601 aattatgcaa ggtaaaataa actcatcggt aacggtatct gtatacgatg actgtatggc
      661 atcggttgca ctgctgaatt ttttgccttc actatttacc atagcattgt aagcaatagc
      721 aatccgctcc catcgcttat cacgatccat ggcataatac ctgccgcaaa ctgttgctat
      781 ttttgctttg aggttttttt gcgaaatata attttccaaa tcggcaataa aggccagccc
      841 gcttttgggg tcggtgtcgc gcccatagtt aaacgcatga ataaagactt ttgcaattcc
      901 ggcttcatgg gctaaaaaac acaaatgctt aagatgttcg atgtgagagt gaacacctcc
      961 gttacttacc aagcccatca agtgtaatgg tttattattt tcgttgcaat aggaaaccgt
     1021 ttttttccat tccacattct ctttgagcaa attatcgcgc acctctttat tcaactttac
     1081 aaggtcttga tacacaattc ttccggcacc aagattcatg tgtccaactt cactattacc
     1141 catttgtccg gcaggcaacc ctacattttc gccatcggta agtaattgtg catgaggata
     1201 ttttttataa caattatcta taaatggggt atgtgcttca ctcactgcat ttgcatgtgt
     1261 ggcgggtgct tcaccccaac catccattat tataagtaca actttctttt tcatcgttaa
     1321 acattttatt tgcaaggtaa agttaccgta aatacacatc cctgaggtac gttatccgtt
     1381 accgaaatag aacctttgtg caattcaaca atatttttta caatatataa acccagtcct
     1441 gtgcctttgg tattgcgtgt ttcttcatta ccaatgcgga aaaacttttc aaatactttt
     1501 tcgcgttcac tttgtttcat acctattcct ttatcggcta ttttaagaat agcattttcg
     1561 ttttgttgtt gtaatgatat caaaacatca acattatcaa aagagtattt aatggcattc
     1621 tcaattaaat tgagcactac cgatgagagt gccatgtcat ctcccagcac cattacatta
     1681 tcttgtatgt tacatttcac tttacggtag ccattatctg tcatttgttt catctgtaca
     1741 atcgattgca ccaaattaga aaggtttatc tgtgaatata caagcgagaa agagtgagta
     1801 tcaattcgcg ctgctgtaag ggcattttcg accaggtcat tcagcctgtc ggtttcggac
     1861 agcgtagatt ctattatgcc ttttgtttgc tctgcactta gtttgtgttt ggccagtgtt
     1921 tgaatattga gctttacggc agccaatggc gatttaaatt catgggttac actcagcaga
     1981 aaattctttt gcattttggc cagggcatat tcttttttaa atgccaaaaa agtctgataa
     2041 ataccgtagc tgagcaaacc gataaaaacg aaaccttcac cggcaatcat ccaccatttg
     2101 gaccgcaatt ttttttcaag atcacttttt agcagcgctt cgttgctatt tccatgcaaa
     2161 tgaacgagtt caaccttgtt ttcatacact tcattattaa gggtgataag catgtatgcc
     2221 caccaaccaa attggagcag gatatacgtt gtgagcacaa taaacagaag ttgaagcctt
     2281 ttcattttga aaggtaaaaa taaaaaaaaa ggacagatat gttaatctgt cccttggatt
     2341 attgtggtaa tagctggaat cgaaccagcg acacaaggat tttcagtcct ttgctctacc
     2401 aactgagcta tattaccgcc ttaattgcga ggcgcaaaag tatattttat aattaattta
     2461 ccaaaagaaa tatgtttcgt tttcatttac actatttcaa ttgataagaa tacatcatgc
     2521 tcataggtta atggttgctt cctgaatcat caattccatc ataaatatct tccatcattt
     2581 tctttcaaag gagggggaat taatcacttt atcaatcaca aaaatgttag tgctcaaaag
     2641 aaaacaacca gcttgcataa cagccaaaaa acaacaatgc ccctgcattt tattgcagag
     2701 gcatgttggt attgaggtca attcttctat tactctatta ctatgcgcat agtgaaatgt
     2761 aacatac
//
LOCUS       DAORQA010000230         2765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8478_length_2765_cov_7.417343, whole genome shotgun sequence.
ACCESSION   DAORQA010000230 DAORQA010000000
VERSION     DAORQA010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8478_length_2765_cov_7.417343"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PK736_08425"
     CDS             complement(<1..414)
                     /locus_tag="PK736_08425"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRC33453.1"
                     /translation="MNIPELNHFFLVGGTCLSLRFGHRKSVDLDLFATNDFDNESLAQ
                     ILENNFKGLSYSSTKNKIGFFCFIDNIKIDFVKHHHFNTIEEPIVEEGIRMFGDKDIL
                     AMKVFAILQRAQKKDFWDIAELLKHYSIVDLIKSYQ"
     gene            complement(423..725)
                     /locus_tag="PK736_08430"
     CDS             complement(423..725)
                     /locus_tag="PK736_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33454.1"
                     /translation="MNSKPKPIFDKRIFWDVNFENIDYDAKAIFVIERVFERGDVDDI
                     RQCRRYYGDEIIKDALINAKWLRLETVYLACAIFDNEPNEYRCYTQAQLSNTPWIY"
     gene            complement(879..1766)
                     /locus_tag="PK736_08435"
     CDS             complement(879..1766)
                     /locus_tag="PK736_08435"
                     /inference="COORDINATES: protein motif:HMM:NF025007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HRC33455.1"
                     /translation="MMATAEKINTMLPGATVDRRENITRKELINEYIIPSKPVILVGA
                     TKNWAAMGKFTPEFFKKNYGHLKKEVKGVTYTIAEFIDLMLASTPEKTAPYPFNLNME
                     TYFPDLMPDIMPDVIYGKSDRIHHPLMPKLMLKGTEVYELFLGGNGSFFPFLHWDALF
                     LHTQITQLYGSKEFILYGPDQTEYMYPRADNTKISSVNIFDPDYEKYPLFRKAKPIRV
                     MVNEGETILFPTKWWHATQMYGPCISLGRVQLNASNWDDFVSDNYKLWQKYHPKMAPL
                     MNGYAKFVGGIMSLQEKFV"
     gene            complement(1791..2576)
                     /locus_tag="PK736_08440"
     CDS             complement(1791..2576)
                     /locus_tag="PK736_08440"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRC33456.1"
                     /translation="MQRIKKIIKKALKIDHTSYLKPLKKVQYIGSHYHGYYIPENLLN
                     ENSVCYCVGAGEDVTFDTELVVNYNAKVFIFDPAPEGINHFAKLNTMVKNGETLSVGS
                     TPFTYRISAPQLDKITYIETGVWSEKTLIKFYKPTVENYASHSIELFNASQEYIEVPV
                     DRLSNLMKDLKHKSIDLFKMEIEGAEYTVIKTIVEDKPDIKIILVEFDEVYHPKDSRY
                     VFRIKEATKSLIEAGYTLAHSTPHYKKLFVRNDVFEALKAKEN"
BASE COUNT          832 a          499 c          447 g          987 t
ORIGIN      
        1 ctgataagat tttatcaaat ccactattga ataatgcttt aacaattctg caatatccca
       61 aaaatctttt ttctgcgctc tttgtaatat agcgaatact ttcatagcta aaatatcttt
      121 atcgccaaac attctaattc cttcttcaac tattggttct tcaattgtat taaaatggtg
      181 atgcttaaca aaatctattt tgatgttatc gataaaacag aaaaagccga ttttattttt
      241 cgtgctactg tacgacaatc ctttaaaatt attttcaaga atttgtgcaa ggctttcatt
      301 gtcgaaatca ttggtggcaa acaaatccaa gtcaacagat tttcggtgac caaaccttaa
      361 tgataaacat gtgccgccaa ccaaaaaaaa atgattgagt tccggaatgt tcattaattc
      421 ttttaatata tccaaggtgt gttgctcaac tgtgcttgtg tgtaacatct gtattcattt
      481 ggttcattat caaaaatagc acatgcaaga taaacagttt cgaggcgcaa ccatttcgca
      541 tttatgagtg catccttaat tatttcatcg ccataatacc tcctgcattg ccttatatca
      601 tccacatcgc cacgttcaaa tacgcgttcg attacaaata ttgctttggc atcgtagtcg
      661 atgttttcga aatttacatc ccaaaaaata cgtttatcaa aaattggttt tggcttgctg
      721 ttcatactac aaagatattg aaaattacag cgtctttgaa ttttgcggtt atgaaaattt
      781 aatgtgcaag aatataagca atgtaaaaaa cttaaccggc aataaagacg atcaactaaa
      841 atagaaaaat caaaagtgaa aatccggaag aaaaatcact aaacgaattt ttcctgaagg
      901 ctcattattc cacctacaaa ttttgcatag ccattcatta aaggagccat tttggggtga
      961 tacttttgcc ataacttgta attgtcgctc acaaaatcgt cccagttaga tgcattaagt
     1021 tgcactctgc caagcgaaat acaagggcca tacatttgtg tagcatgcca ccattttgta
     1081 ggaaacaata ttgtttcgcc ttcattcacc attacacgaa taggcttagc ttttctaaac
     1141 aagggatatt tttcgtaatc gggatcgaaa atatttaccg atgatatttt tgtgttatcg
     1201 gcacggggat acatgtattc agtttgatcg ggaccataga gaataaattc tttgctgcca
     1261 tacaattgtg taatttgtgt gtggagaaaa agtgcgtccc aatgaagaaa agggaaaaaa
     1321 gaaccattac cacccaaaaa taattcatat acttctgtac ccttgagcat taattttggc
     1381 atcagtggat gatgaatcct atccgattta ccataaatta catcgggcat tatatcgggc
     1441 atcaaatcgg ggaaatatgt ttccatattt aagttgaaag gataaggtgc tgttttttcg
     1501 ggagtagaag caagcatcaa atcgataaat tcagctatgg tatatgttac tccttttact
     1561 tcctttttaa ggtgaccgta attcttttta aaaaactccg gtgtgaattt tcccatggct
     1621 gcccagttct ttgtggcacc aaccaatatt acaggttttg aaggaattat gtattcattt
     1681 attaattcct ttcgtgttat attttctctc ctgtctacgg ttgcacccgg tagcatggta
     1741 tttatttttt ctgctgttgc catcatattt tataaagttt aaaatggtta ttaattttct
     1801 tttgccttca atgcttcgaa cacatcgttg cgaacaaata attttttgta atgcggtgtt
     1861 gagtgtgcaa gtgtgtaacc ggcctctatc aaactttttg ttgcttcttt tattctgaaa
     1921 acatacctcg aatctttagg atgatacacc tcgtcaaact ccaccaatat tatttttata
     1981 tccggcttat cttctactat cgtttttatc acagtatact ctgcaccttc aatttccatc
     2041 ttaaacaaat caatagattt atgttttaaa tctttcatca aattcgaaag cctgtcaacc
     2101 ggtacttcta tatattcttg cgatgcatta aataattcta tggaatgcga agcataattt
     2161 tctacagtag gtttgtaaaa tttaatcaat gttttttcgc tccatactcc tgtttcaatg
     2221 taagtaattt tatcgagctg tggtgccgat attctgtaag tgaaaggtgt agatcctacg
     2281 gagagcgttt ctccgttttt taccatcgta ttgagttttg caaaatgatt tattccttcg
     2341 ggcgcagggt caaaaataaa tactttggca ttgtaattta ccaccaactc ggtatcgaaa
     2401 gtaacatctt cgccagcacc tacgcaatag caaacagaat tttcgtttaa taaattttca
     2461 gggatataat aaccgtgata atgcgaacct atgtattgca ctttctttaa tggcttgagg
     2521 tacgatgtgt ggtctatctt aagcgccttc tttattattt tttttatcct ttgcatacaa
     2581 tatttttgca ataataaaca atttaatctt tgccactatg ccatttatcc ttatgtgtta
     2641 tgcgattttt aaacaatgta ggttgcgggt gttttttttt ataattatcg aaatgcaaag
     2701 cttaacattg tttttgtttt ttcgtgccat atacatttat agagttaagg tgtcatttta
     2761 ctgct
//
LOCUS       DAORQA010000231         2758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8508_length_2758_cov_6.049945, whole genome shotgun sequence.
ACCESSION   DAORQA010000231 DAORQA010000000
VERSION     DAORQA010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8508_length_2758_cov_6.049945"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PK736_08445"
     CDS             complement(<1..500)
                     /locus_tag="PK736_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRC33457.1"
                     /translation="MTAKFSLSKIGNVRSLTLIAALACICIVFHFATDEVFLSARNFS
                     NLMRQASVVGIVACGMVMIIVSREIDLSAGSAVALAGGLAAIANVQFQMHPAMAIGVA
                     LAAGLLIGAINGLLVAYTRIPAFIVTLGGLLVYRGLIKGFTQGETIGPVSEGFQMFGT
                     GFVPSY"
     gene            complement(500..>920)
                     /locus_tag="PK736_08450"
     CDS             complement(500..>920)
                     /locus_tag="PK736_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC33458.1"
                     /translation="LKSFTANNLMQKKEMESYANENIQQLRIKTNDAATKTGSLSGGN
                     QQKVVFSKWMKKLPHLLLADEPTRGIDVGGKAEIYKEIYRLARQGTAVLVVSSDLLEL
                     IGICDRMYVMANGEIKVCLNRNEFDEKIILKHALNIS"
     assembly_gap    921..957
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<958..1995)
                     /locus_tag="PK736_08455"
     CDS             complement(<958..1995)
                     /locus_tag="PK736_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC33459.1"
                     /translation="MYLQIKNINKFYGNTHALSNVSFDVRKGTIHAICGENGAGKSTL
                     IKILCGLIPHGQYEGSFTIDGVEKKFFTINDSRKSRIAVIAQELALVPEMTVAENIFL
                     GNEPRKNFIVDKDKMYDLASQAMLQLQCDIGTHTQVKELGIGKQQMIEIAKAISKNAE
                     ILILDEPTAALTEQESTTLLNLLLQLKDKGVTCILISHKLKEILKVSDDITVLRDGST
                     VCTMKISETTEIDLVSKMVGRTMVDFFSKEKIDIGKEVLQIKNLSSTLRNKKVMHSNV
                     NVNVHAGEIVGIAGLMGSGRTELLQILFGAHEFIVDGSIVLNGDKILPQNPQQAIEKG
                     IALITEDRKQFG"
     gene            complement(2005..>2758)
                     /locus_tag="PK736_08460"
     CDS             complement(2005..>2758)
                     /locus_tag="PK736_08460"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRC33460.1"
                     /translation="ILIVPHNSKTCATAVKQANEANIPIIAYDRMIKDCDISMYISFD
                     NEKVGEMQATYATQVAPKGNYLLAGGSATDENAILYRKGQMNILQPFVDKGEIKILID
                     QFCKDWLPVEALKLTQNALTLSNNTIAAIVVANDGLAGGCIQALTEQQLQGKVPVTGQ
                     DAEISAIQRILDGTQSMTVYKPVKILAETAAEVAMGLVKKTSTIAETKKMNNGVKDVP
                     SILLTPVMVTKENIEATVIKDGYIDKSKLKFQ"
BASE COUNT          720 a          595 c          485 g          921 t
ORIGIN      
        1 atatacgatg gtacaaaccc ggttccaaac atttgaaaac cctcgctcac cgggccaatg
       61 gtttcgccct gtgtaaaacc ttttataaga cctctgtaaa ccagtaaacc gccaagggta
      121 acaataaatg cagggatgcg cgtataggcc accaatagtc cgtttatagc gccaataagc
      181 aatccggcag ctaaggctac tcctatggcc atggcgggat gcatttgaaa ttgcacattt
      241 gcaatggccg ccaatcctcc ggcaagtgct actgccgaac ctgcagaaag gtcaatttcg
      301 cgcgatacaa ttatcatcac cattccgcaa gcaacaatgc ccacaacgga tgcctgtcgc
      361 atgaggttcg aaaaatttcg tgccgataga aatacttcat cggtggcaaa gtgaaatacg
      421 atacatatac aagccaatgc tgctattaaa gttaacgagc gtacattgcc aattttactg
      481 agagaaaatt ttgctgtcat tatgaaatat ttaaagcgtg ttttaaaatt attttttcat
      541 caaattcatt ccggttgagg caaactttta tttcaccatt tgccattaca tacattcggt
      601 cgcatatgcc tatcaactcc agtaggtcgg acgatactac caaaaccgca gttccctgcc
      661 gcgccaaccg ataaatctct ttataaattt cggccttgcc gcctacatct attccacgtg
      721 tgggttcatc ggcaagcaat agatgaggca gttttttcat ccatttacta aacacaactt
      781 tctgctgatt gccaccgcta agacttccgg ttttggtggc ggcatcattt gtttttattc
      841 ttagttgctg tatgttttcg ttggcataac tttccatttc ctttttttgc atcaggttgt
      901 ttgcagtaaa gctttttaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacc
      961 aaactgctta cggtcttcgg taattaatgc tattcctttt tcgatggctt gttgcggatt
     1021 ttgtggtaga attttatctc cgttcaaaac tatactgcca tccacaatga actcatgggc
     1081 accaaataat atttgcaaaa gttcggtgcg gccgcttccc atcaggcctg caatgcctac
     1141 tatttcacct gcatgtacat tcacattcac atttgaatgc atcacttttt tattgcgcaa
     1201 agtggagctc aaatttttta tttgcaatac ttctttacca atgtcaattt tttctttact
     1261 gaaaaaatcg accatagtac gacccaccat ttttgatacc aaatctattt cggtggtttc
     1321 acttattttc atggtgcaca ctgttgaacc atcgcgcagc acagtgatat catccgatac
     1381 ttttaatatt tctttgagct tatgagaaat aagaatgcac gtaacacctt tgtcttttaa
     1441 ttgtaaaagt aaattcaaaa gcgttgtcga ttcctgttct gtaagtgcag cagtaggttc
     1501 gtcaagaatt aatatttcgg catttttcga aatggccttt gcaatttcaa tcatctgttg
     1561 cttaccaatt cccaattctt ttacctgtgt atgtgtacct atatcgcatt gcaattgaag
     1621 catagcttgc gaagccaggt catacatttt atctttatcg acaataaaat ttttacgggg
     1681 ttcgttgcca agaaaaatat tttcggccac cgtcatctcg ggcaccaatg caagttcctg
     1741 tgcgataaca gctatgcggc ttttacgcga atcatttatg gtaaagaatt ttttttctac
     1801 accatcaatc gtaaaacttc cttcatactg gccatgaggt ataaggccgc acaagatttt
     1861 tatcaacgta gatttgccgg caccattctc accacatata gcatgtatgg ttcctttgcg
     1921 cacatcgaac gaaacattgc ttaatgcatg tgtattgccg taaaacttat ttatattttt
     1981 tatctgtaag tacatgacat ttaattattg aaacttcaat ttcgatttgt ctatatatcc
     2041 atctttaatt acggtggctt caatgttttc ttttgttacc attaccggtg ttaacaaaat
     2101 gcttggtaca tctttcacac cattgttcat tttttttgtt tcggcaattg ttgatgtttt
     2161 tttcactaag cccattgcaa cttcggctgc tgtttcggca agtatcttta caggcttata
     2221 tacagtcata ctttgtgtac catccaatat gcgttgtatg gccgaaattt ctgcatcctg
     2281 tccagtaaca ggtactttgc cttgcaactg ctgctcagtg agggcttgta tgcatccacc
     2341 tgccaggcca tcgtttgcaa ctactattgc agcaattgta ttattggaaa gtgtaagtgc
     2401 attttgtgta agtttcaaag cctcaacagg caaccaatct ttgcaaaatt gatctatcaa
     2461 tatttttatt tcgcctttat cgacaaatgg ttgtaaaata ttcatttgac ctttgcggta
     2521 taatattgca ttctcgtccg tggccgatcc tccggccaat aaataatttc cttttggcgc
     2581 aacctgtgtt gcgtatgttg cctgcatttc gccaaccttt tcattatcga aagatatgta
     2641 catacttata tcgcaatctt ttatcattct gtcgtaagca atgatgggta tattggcttc
     2701 gttggcttgt tttactgcag tagcgcatgt cttgctgttg tgaggcacta tcagaata
//
LOCUS       DAORQA010000232         2757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8512_length_2757_cov_5.796077, whole genome shotgun sequence.
ACCESSION   DAORQA010000232 DAORQA010000000
VERSION     DAORQA010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8512_length_2757_cov_5.796077"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..579
                     /locus_tag="PK736_08465"
     CDS             22..579
                     /locus_tag="PK736_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HRC33461.1"
                     /translation="MEWKQIVLSLLMGLGLSACCGLRVFVPLLLAGIAHRMGWLSVND
                     QFMWLSNYTSIAGFGIASVVEILAYKIPFIDHLLDTITTPASIVAGTILSASTLFNID
                     PMMQWTLALLVGGGSAGLLQTATTLLRGTSTATTGGTANPFFAMVESTIAIVASIISI
                     ILPLIAGIIFLVVCFFVFRRIRRKR"
     gene            complement(736..806)
                     /locus_tag="PK736_08470"
     tRNA            complement(736..806)
                     /locus_tag="PK736_08470"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(772..774),aa:Gln,seq:ttg)
     gene            complement(853..1239)
                     /locus_tag="PK736_08475"
     CDS             complement(853..1239)
                     /locus_tag="PK736_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33462.1"
                     /translation="MKDELIGYAKKLVLTGLLATLIIYLVQMVLPAKWQVPAPWVLVV
                     FFFIMCLGFYAGLLFSSEGNPKGFVRFYMVGTFLKLLIFLGVIIFFGLIKPDFALGFT
                     FNFFIIYLVFTVFEVAFLYQRFKKNE"
     gene            complement(1271..2515)
                     /locus_tag="PK736_08480"
     CDS             complement(1271..2515)
                     /locus_tag="PK736_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRC33463.1"
                     /translation="MRSGDRIVKIEDEVVAGIKIKNNDVIKRLRGDKGTKVKVSILRR
                     GERELLDYVITRDKIPLFSVDASYVIAPKTGYIKISRFADSTVEEFKTALDSLKQQGI
                     ENLILDLQGNGGGYLSRAIELADEFLSDKTLVVYTEGRNNPRQENLSTSIGGWEKGRL
                     AILVDESSASASEIVSGAIQDWDRGLIVGRRSFGKGLVQKPFGLPDGSAVRLTIARYY
                     TPSGRCIQKSYSNGDEDYDLELAERTKRGELFYADSLPKIDSLKKFTNNKRLVYGGGG
                     ITPDVFVPLDTNMNSKYYRDLVRKGLINEYTLDYMDSHRKELVAAYSDVNTFKNQFVV
                     DDKLMDDFFAYCDKHDLKKDEEGYKISKVIIQASIKGLIARNLWNLNAYFVILNDEND
                     AAQAAIKAMTDKSFEKNKIAWK"
     gene            complement(2525..>2757)
                     /locus_tag="PK736_08485"
     CDS             complement(2525..>2757)
                     /locus_tag="PK736_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HRC33464.1"
                     /translation="QMVDDYYVDTLNESKIVDDGIRAILKDLDPHSVYIPSDELKQTN
                     EPLVGKFEGVGIQFNILYDTILVSNTVPGGPS"
BASE COUNT          832 a          550 c          511 g          864 t
ORIGIN      
        1 ctttgaaata aaatatacac tatggagtgg aaacaaattg tcttaagttt attgatgggt
       61 ttaggtctga gcgcatgttg tggactgcgt gtatttgtgc ctttgctact tgccggcatt
      121 gcgcaccgca tgggatggct gagtgtaaat gaccagttta tgtggcttag caattataca
      181 agtattgcgg gttttggcat tgcatctgtt gtagaaatat tagcatacaa aattccattt
      241 atcgatcact tgctcgatac cattactaca ccagccagta tagttgcagg cacaattttg
      301 agtgccagta cactattcaa tatcgacccg atgatgcagt ggacacttgc attgcttgtg
      361 ggtggtggca gtgcaggatt gctacaaacg gcaactacat tgcttcgagg aacatcgaca
      421 gcaacaacgg gaggaactgc caatccgttt tttgcaatgg tagaaagcac aattgccatt
      481 gttgctagta ttatttcaat catacttcct ttaattgcag gaattatttt ccttgtggtt
      541 tgcttttttg tttttcggag gattaggcgg aaaagataaa gtatcatagc ttgattgatt
      601 gcaagtttgt gaaatttgta aatcaatttg aaaagagaat aacgaaaacc agttgatttg
      661 ggaaggcaaa aaacaaatca caatttaaaa caaaaaatcc cctgataatt tatatcaagg
      721 gacttttata ttttgttgac cgactagggc tcgaacctag aatgacagaa tcaaaatctg
      781 ctgtgttacc attacaccat cggtcaataa aacggagcgc gaaagtacaa ataaaagtaa
      841 aaaacaaaaa ctctattcgt tttttttaaa acgttgatac aaaaatgcca cttcaaaaac
      901 tgtaaatacc agataaatga taaagaagtt gaaagtgaaa cccagggcaa agtcaggttt
      961 tataagacca aagaagataa ttacgcctaa aaatattaat agctttaaaa acgtgcctac
     1021 catgtaaaac cgcacaaatc cttttgggtt gccctcggag ctaaataata aacctgcata
     1081 aaagcctaag cacataataa agaaaaaaac caccaatacc catggtgcag gtacttgcca
     1141 ttttgcaggt aataccattt gcacaaggta aattatgagt gtggccaaca ggccggtgag
     1201 cacgagtttt ttcgcgtagc ctataagttc gtctttcaat gtttttggtt ttaggagcgt
     1261 gcttaaaaaa ttatttccag gcaattttat tcttttcgaa actcttatca gtcatggctt
     1321 ttatagcggc ctgtgcagca tcattttcat catttagtat cacaaaataa gcattcaaat
     1381 tccataggtt acgtgcaata aggcccttga tggatgcttg aataattact ttcgaaattt
     1441 tatatccttc ctcatctttt ttcaaatcat gcttatcgca ataggcaaaa aaatcgtcca
     1501 tgagtttatc atctactaca aattgatttt tgaaagtatt gacatcgcta taagctgcta
     1561 ctaactcttt gcggtggcta tccatgtagt cgagtgtgta ctcattgatg aggcctttac
     1621 gtaccagatc gcgataatac ttgctgttca tattggtatc gagtggtaca aatacatctg
     1681 gcgtaattcc accgccacca taaactaatc gtttattatt tgtaaacttt tttaaagaat
     1741 cgatttttgg aagactatca gcataaaata attcaccacg ctttgtacgc tctgccaatt
     1801 caaggtcgta atcctcatcg ccattggaat aagatttttg tatacaacga cccgatggag
     1861 tataatatcg ggcaatggtt aatcgcactg ccgaaccatc aggcaagcca aaaggctttt
     1921 gaaccaatcc tttaccaaac gatctacggc caacaataag tccacgatcc caatcttgaa
     1981 tggcgcccga aactatctca cttgccgatg ccgatgattc gtcaaccagt attgctaatc
     2041 gtcctttttc ccagccgcct atgcttgtag aaagattttc ttgccgtggg ttgttgcgcc
     2101 cttcggtata tactacgagt gttttgtcgc tcaaaaactc atctgcaagc tctatagcac
     2161 gtgataaata tccaccaccg tttccttgta aatcaagaat gaggttttct ataccttgtt
     2221 gtttcaatga gtcgagcgca gttttaaatt cttcaacagt agaatcggca aatcgcgaaa
     2281 tttttatgta tccagttttt ggtgctatga catacgaggc atcaacacta aataatggaa
     2341 ttttatcgcg agtaataaca taatccaata actcgcgttc gcctctgcgc aaaatactta
     2401 cttttacttt tgtgccttta tcgccacgca gtctttttat tacatcattg tttttaatct
     2461 ttattccagc caccacttca tcttcaattt ttacaatgcg gtcgccactg cgtattccaa
     2521 gcttttagga cgggccaccc ggaactgtgt tgcttactaa aatggtatca tacaggatat
     2581 taaactgaat gcctactcct tcaaatttgc ctacaagagg ttcgtttgtt tgtttaagtt
     2641 catcggatgg aatgtatact gagtgagggt cgaggtcttt caaaattgcc cgaatgccat
     2701 cgtccacaat tttgctttca tttaatgtat caacataata gtcatccacc atttgaa
//
LOCUS       DAORQA010000233         2739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8581_length_2739_cov_16.004098, whole genome shotgun sequence.
ACCESSION   DAORQA010000233 DAORQA010000000
VERSION     DAORQA010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8581_length_2739_cov_16.004098"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="PK736_08490"
     CDS             <1..1000
                     /locus_tag="PK736_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRC33465.1"
                     /translation="NGAKIFFNHRVTNYDINNNIITIEDATNKKTFQISPGLTIGSDG
                     AFSSSRLGIQFTDRCNYSQTYLEHGYKELVIEAGPDNSFLIEKNALHIWPRGGYMLIA
                     LPNPEGSFTCTLFFPFEGNPSFSMLKTEADVLQFFEQQFPDALKLMPNLAHDFFANPT
                     GSLVTVRCKPWSFNNKLLLIGDAAHAIVPFYGQGMNCGFEDCFTLNEMITKHAHNWSA
                     IIHEFEALRKPAADGIAELALMNFIEMRDLVGSAKFLLRKKIEGAFYTKYPEKWQPLY
                     TMVTFSHLPYHEALAMGKFQDTIMDKVMELPDIENIWNTQKVEDMMLQLLAESKKS"
     gene            complement(1012..1719)
                     /gene="lipB"
                     /locus_tag="PK736_08495"
     CDS             complement(1012..1719)
                     /gene="lipB"
                     /locus_tag="PK736_08495"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRC33466.1"
                     /translation="MQQIQYIDWGLIDYKEAWEQQEQLMQNIIAIKMHNRNLENAAHE
                     ILPHKFIMCEHPHVYTLGKSGSEKNLLRTTDELEKIDASFYKINRGGDITYHGPGQLV
                     GYPILDLDCFFNDIHKYMRCLEEVIILTLKNYNIIGTRIAGLTGVWLQAGGGKPERKI
                     CAMGVRLSRWVTMHGFALNANNDLSYFNNIIPCGIDDKAVTSIMEETGESVDIEKLKK
                     QILQNFANVFEAEITLA"
     gene            1849..2619
                     /locus_tag="PK736_08500"
     CDS             1849..2619
                     /locus_tag="PK736_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRC33467.1"
                     /translation="MTNKIQALIIEDEIQSANTLRLIVNEYCPNVEIVGHATSVKEAI
                     ELTDSLRPQLLFLDVEIQGGTAFDVLVKIEHKKLNIIFITAYDSYALQAIKYSAIDYL
                     LKPIDIEELKNAIGKLQSHTVGDNDSTIISQFIDVLKNNARNPSSFIPIPFNDGFSFI
                     PISEIVRLEASGSYTYIFVNGKDKILASKNLKEYESMLPEDTFIRVHNSHLLNKAHIR
                     KYVKGSGGYCIMSDGTTVSIATRKKEWFLRMMGIGGAE"
BASE COUNT          833 a          548 c          524 g          834 t
ORIGIN      
        1 gaatggcgca aaaatatttt tcaatcaccg cgtaacaaat tacgatatca acaataatat
       61 tatcaccatt gaagatgcca ccaataaaaa gacatttcaa atttcgccag gcctcacaat
      121 aggcagcgat ggcgctttct catcatcgcg attaggaatt cagtttaccg acaggtgcaa
      181 ttattcgcaa acctatttag aacatggtta caaagagttg gttatagaag ccggtcccga
      241 caattctttt ttgatagaaa aaaatgcctt gcacatttgg cctcgtggtg gttacatgct
      301 tattgcattg cccaatcccg aaggaagttt tacctgcaca ttgttttttc ctttcgaagg
      361 caatccgtca ttttcaatgc ttaaaactga agccgatgtg ttgcagtttt tcgaacagca
      421 atttcccgat gcattaaaat tgatgcccaa tttagcgcat gattttttcg caaatcctac
      481 cggttcgctg gtaactgtgc gttgcaaacc atggtcgttc aataacaaac ttctgttgat
      541 tggcgatgcg gcacatgcca ttgtaccttt ttatgggcag ggaatgaatt gtggtttcga
      601 ggattgtttt acgcttaatg agatgattac aaaacatgca cataactgga gtgccattat
      661 tcatgaattt gaagccttgc gcaaacccgc tgccgatggt attgccgaac ttgctttgat
      721 gaattttatt gaaatgcgtg accttgttgg cagtgcaaaa tttttgttgc gcaagaaaat
      781 tgaaggagca ttctatacta aatatcccga aaaatggcag cctctttata ccatggtaac
      841 cttcagtcat ttaccttatc atgaagcatt ggcaatggga aaatttcaag ataccatcat
      901 ggacaaagtg atggaattac ccgatatcga aaatatttgg aatacacaaa aggtagaaga
      961 tatgatgttg caattgctgg cggaaagcaa aaaaagctaa taaaaaattc gctaggctaa
     1021 agtaatttca gcttcgaaaa cgttggcaaa gttttgaagt atttgttttt ttagtttttc
     1081 tatatcaaca ctttctccgg tttcttccat tattgatgtt accgctttat cgtcaatacc
     1141 acagggaata atattattaa aatacgataa atcgttgttg gcattcaatg caaaaccgtg
     1201 catggttacc catcgcgaaa ggcgaacacc catagcgcaa attttacgtt cgggcttgcc
     1261 accaccggct tgcagccaca caccggttaa gcctgcaatg cgggttccaa taatattata
     1321 atttttcagg gtgagaataa tcacttcttc aaggcaccgc atgtacttat gtatatcgtt
     1381 aaagaagcaa tccaaatcca aaatgggata acccaccaat tggcccggcc cgtgataagt
     1441 aatatcgcca ccacgattta ttttataaaa tgaagcatca attttttcaa gttcatcggt
     1501 tgttcgcaaa agattttttt cgctaccgct tttacccaac gtatacacat gtggatgttc
     1561 gcacataata aatttgtggg gtaatatttc atgggctgca ttttccaaat tgcggttgtg
     1621 catttttata gcaataatgt tctgcatgag ttgttcctgt tgttcccaag cttctttata
     1681 atctattaaa ccccaatcga tgtattgtat ctgctgcact atactttatt ttgcggcaaa
     1741 actaatttta ttcgtccggt gttttacaaa aagatattgt tgggtaaaat atttttttag
     1801 ttttgtaaca tctttctgta accgtgtaat cgcacatgaa cattaataat gaccaataaa
     1861 atccaggcac tgataattga agacgaaata caatcggcca acacactcag gcttatagta
     1921 aacgaatatt gccccaatgt tgaaattgtg ggccatgcca catcagtaaa ggaagccatt
     1981 gaacttaccg atagtttgcg tccgcagtta ttgtttctcg atgtcgaaat tcagggcgga
     2041 acagccttcg atgtccttgt taaaatagaa cacaaaaaac tcaacatcat ttttattacc
     2101 gcctacgaca gttatgcatt gcaggccata aaatattctg ccatcgatta tttgctaaaa
     2161 cccattgata ttgaagagct aaaaaatgcc atcggcaaat tgcaaagtca tactgtgggc
     2221 gataacgata gcaccatcat ttcgcagttt atcgatgtgc tgaaaaataa tgcacgcaat
     2281 ccttcgtctt ttattcccat tccttttaat gatggattta gttttattcc cattagcgaa
     2341 attgtgcgcc tcgaagcatc gggaagttac acgtatattt ttgtgaatgg taaagataaa
     2401 attctcgcga gcaaaaacct gaaagaatac gaatcgatgt tgcccgaaga cactttcatt
     2461 cgtgtacaca attcgcattt acttaacaaa gcacacatca gaaaatatgt gaaaggaagc
     2521 ggaggatatt gcatcatgag tgatggtaca acggtatcga tagcaacccg taaaaaagaa
     2581 tggttcctgc gcatgatggg cattggtggt gctgaatgac gtgtgacgtg ttttttgtcc
     2641 actgttgacc cggataaaaa ccccacaaaa aaaccggcaa aatttttatg tcctcggttg
     2701 acgtccccgc caaccgctat cgattcgcgt gataattgt
//
LOCUS       DAORQA010000234         2712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8679_length_2712_cov_4.522770, whole genome shotgun sequence.
ACCESSION   DAORQA010000234 DAORQA010000000
VERSION     DAORQA010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8679_length_2712_cov_4.522770"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..2332
                     /locus_tag="PK736_08505"
     CDS             77..2332
                     /locus_tag="PK736_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HRC33468.1"
                     /translation="MKKVNHIKKIIALITIIASMQISVAQQRKATTLDDIAETNLHTA
                     WIHFKPESKIEAATIFEKYTFNFGLSAQDEMKLFRTNKMPFEGLVINRYQQYHNGYKV
                     IGATINIHERDGIAQKANGRLLKNFNSSISVPAYTETNALNNALQFIPAKDYAWLDTR
                     KVAKVRAKKNDPTITLYPKGELVYVQPKGKKDFEAADFILCWKFDVAMSRSNSQRVFI
                     NANTGSVVNSYPLDATCSGTTIDLPYNGNRTVYTSQQGRCANGNYYEMWDDCQSAYIH
                     VEDYDTGDPKCNYVPGNTWTGLIGTTSDAAQINWGMRQTYNYHLNNFNWTSYDDASGS
                     IDCTSGFNFIDDNGQVSGNNARWTWVDEDFDFGYGASGIADDDSYTTLDIVAHEFTHG
                     VDEYSANLDYSGESGALDESFADIFGEIVEQSIENNWATPSWTLAEDLTNKDYIRSFI
                     APKIKGDPDTYLGTNWYTGSDGNKEVHTNSGVQNHFFYVLVEGKSGVNDLGNQYNVSG
                     IGINDAKDIAWLAHQYLFDAAQYIDSRDAWLEAAGYLFGSCSNQAIQVGNAWHAVGVG
                     AQSPFYTKFATGTLTAGVSNKLYEAISHVYSVALTTVNSTSGAKEVRFYAGDEVTLNT
                     GFTAPSGCAFVAKINPCSVTLHSRNSNAANAEVNETPANFESPNPMSVVPNPFQNSFT
                     CRLTSVNDNENVTITIYDAMGKSAILQKRIIAQGRNELLVDASTLLPGIYHVQITTDS
                     GNTYQQKILKQ"
BASE COUNT          893 a          541 c          545 g          733 t
ORIGIN      
        1 tccttccccc tctccaatgg agagggccgg ggtgaggttc ttaaaattct aaaaaaacgc
       61 atctaaaaaa aacaaaatga aaaaagtaaa tcacatcaaa aaaataatcg cgctcatcac
      121 cattattgca agtatgcaaa tatcggtggc acaacagcgc aaggcaacta cactcgatga
      181 tattgccgaa acaaatctcc acacagcatg gatacatttt aaacctgaat cgaaaataga
      241 agccgccacc atattcgaaa aatatacatt taactttggc ttatctgcac aggatgaaat
      301 gaaattattt cgtaccaaca aaatgccatt cgaaggactt gttatcaacc ggtaccaaca
      361 ataccataac ggctacaaag taataggtgc aaccatcaat atacatgagc gcgatggtat
      421 agcacaaaaa gcaaatggac gtctgttaaa aaacttcaac agcagcatta gcgtgccagc
      481 gtatacagaa accaatgcac tgaacaatgc tttacaattt attcctgcaa aagactatgc
      541 atggctcgat acaaggaagg ttgcaaaagt acgtgcaaag aaaaacgacc ccacaataac
      601 gttgtatcca aaaggcgaac tggtatatgt gcagccaaaa ggcaaaaagg atttcgaagc
      661 cgcagatttt attttgtgct ggaaatttga tgtagccatg agtcgcagta atagccaacg
      721 cgtttttatt aatgcaaata ccggcagtgt tgtaaacagc tatccactcg atgctacctg
      781 cagcggcaca actatcgact tgccttacaa cggtaatcgt actgtttata catcgcaaca
      841 aggcagatgt gccaatggaa actattatga aatgtgggac gattgtcagt cagcgtatat
      901 ccacgtggaa gattacgaca cgggcgatcc caaatgcaat tacgtacccg gcaatacatg
      961 gacagggttg attggtacaa caagcgatgc agcgcaaata aactggggta tgcgccaaac
     1021 gtataactac catctcaata attttaactg gacaagctat gatgatgcaa gtggcagtat
     1081 cgactgcaca tcgggcttca attttattga cgacaatggg caggtgagcg gcaataatgc
     1141 gcggtggaca tgggtagatg aagattttga ttttggttat ggggcgtcag gtattgctga
     1201 tgacgattcg tatacaacgc tcgatatagt tgcacatgag tttactcacg gagtagatga
     1261 gtactctgca aacctcgatt acagtggtga gtcgggcgca ttagacgaat cgtttgccga
     1321 tatatttggc gaaattgtag agcagtctat agaaaataac tgggccacac catcgtggac
     1381 attggccgaa gaccttacta acaaagacta tatacgttcg ttcattgcac ccaaaataaa
     1441 aggagacccc gatacttacc ttggcactaa ctggtacaca ggcagcgatg gcaataaaga
     1501 agtgcatacc aatagcggtg ttcaaaatca ttttttctat gtactggttg aaggcaaaag
     1561 cggagtaaat gatttaggaa atcaatataa tgtatcgggc attggcataa atgatgcaaa
     1621 agatattgca tggcttgccc accaatattt atttgatgcc gcacagtata tcgattcgcg
     1681 cgatgcatgg ctcgaagctg ctggttattt gtttggttct tgttccaatc aagccattca
     1741 ggttggcaat gcatggcatg ctgtaggcgt gggtgcgcaa agtccttttt atacaaagtt
     1801 tgcaacaggc acactcaccg caggtgtatc aaacaaatta tatgaggcca tcagtcatgt
     1861 ttattctgtt gctttaacaa cggtaaattc aacttcggga gcaaaggaag taaggtttta
     1921 tgccggtgac gaggttacat tgaacacagg ctttacggca ccaagtggct gcgcttttgt
     1981 tgcaaaaata aatccttgca gcgttactct tcactcgcgc aatagcaatg ctgccaacgc
     2041 tgaagttaat gagactcctg caaatttcga aagcccaaat ccaatgtccg ttgtacccaa
     2101 tccgtttcaa aatagtttca cttgtaggct tacatcagtc aacgacaatg aaaatgttac
     2161 cataactata tacgatgcta tgggtaaaag tgctatttta caaaaaagaa tcattgcaca
     2221 aggccgcaac gaattgttgg tcgatgcctc tactctatta ccgggcatat accatgtgca
     2281 aataacaact gatagtggca acacctatca acaaaaaata ctcaaacaat aaacgtccac
     2341 acgaaaacat tttttcattt acagaaaggc cggatttatt ccggtctttt tttttacact
     2401 aatatttgtt cttacaatgt taaaacctgc tacatataat aattcgaaac aaaaatttgt
     2461 attacttcct tagcattgct tggcgaaatt tcttatcgaa aaacaaaaga ctcttgtatg
     2521 taacaaatat ttttttggag gttcataaat attagagcat atgattattt gttcttacat
     2581 atattataaa aacaatcttt tgattttcta tgtctgcagt gtcgtagcaa tttctatttt
     2641 taccacatga aattgactaa caggttagga ttgattggtt ttttaggaga ctgacattac
     2701 gcacaataaa at
//
LOCUS       DAORQA010000235         2701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8720_length_2701_cov_4.179138, whole genome shotgun sequence.
ACCESSION   DAORQA010000235 DAORQA010000000
VERSION     DAORQA010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8720_length_2701_cov_4.179138"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1782
                     /locus_tag="PK736_08510"
     CDS             151..1782
                     /locus_tag="PK736_08510"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HRC33469.1"
                     /translation="MKLKLKFASVTLLFAMSNILISQAQSVPAGIHGNFQSDVQYYRV
                     DSSIGAPIVPQKVLTNGFANINYTNNNFSCGLRYESYLNVLQGYDARFKGNGILYRYA
                     QYNKDNLDVTVGSFYEQFGSGMILRSYEERGLGYDNAFDGVRVKYNPTPGLYMKGFIA
                     RQRAYFDYGPGIVRGFDTELNFAELMPKASEKGWQLSLGGSFVSKYQKDEDALKKLPE
                     NVGAFSTRANLGYKGFTFNAEYAYKYNDPSAVNTFIYKEGQSFLTSFGYTKKGLGINL
                     TAKRIDNMDYRSDRTETVSTLLINFMPAIARQYTYRLLTLYPYATQPMGEMGAMAELY
                     YTFKPNTGLGGKTGMYMAINSTYVNNLKATPDTNGYGYTSNFLAIGKEHYYADVNVEL
                     TKKLSKKAKITLNYVYIEYNKNVLEGKLDYGVIYSHTGILDFTYKFNHTKSIRTELQH
                     LYTKQDLGSWAMALVEFSMAPHWSVAAFAEYNYGNKKPDDRFFYPNVSSTYVHNSNRF
                     SLSYGKQRAGLLCVGGVCRFVPASNGVSFTVSSSF"
     gene            1801..>2330
                     /locus_tag="PK736_08515"
     CDS             1801..>2330
                     /locus_tag="PK736_08515"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33470.1"
                     /translation="MTNKIKFSFFFAIMVLATIIASCDKVSPPFDETGSGGNPNPDVT
                     VQKVLIEDFTGHTCKNCPAASKIAENIQKAYPGRVVVMAIHSGFYADTGATGNFTYNF
                     RIPEGQQISDDFGISSWPNGMVNRRKYNGSPITGKDSWSSHTGLWLQETPKASIKISN
                     VYNDATRNITTTITSKA"
     assembly_gap    2331..2378
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2379..2631
                     /locus_tag="PK736_08520"
     CDS             <2379..2631
                     /locus_tag="PK736_08520"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HRC33471.1"
                     /translation="IIKPQIVDNTTVLDYVHMHALRGAFNGAYGEVVMNNPATNDSIV
                     KTYSLTLRADAVAKNCHVVAYIIDETSKEIIQVEEKEMK"
BASE COUNT          849 a          464 c          546 g          794 t
ORIGIN      
        1 gagtaatacc acactaaatt atttttagcc ggttttcttt tatataaaaa taatattgca
       61 aaaaaagctc atctttcaaa agatacactt tgttttctgt tttgcttatt caaaaaatat
      121 atatttacac aaaatatttt ccaccttttt atgaaactta aactaaaatt tgcttcggtt
      181 acattgttat ttgcgatgag taacattttg atttcccaag cccagtcggt gcctgcaggc
      241 atacatggta attttcaaag cgatgttcaa tattacagag tcgactcgag tattggcgct
      301 cccattgtgc cacaaaaagt gctgacaaat ggttttgcaa atatcaatta tacaaacaat
      361 aatttttcgt gcggccttcg ttacgagtca tatttaaatg tgttgcaagg gtatgatgcc
      421 cgctttaaag gcaatggtat tttgtatcgc tatgcacagt ataacaaaga taatttagat
      481 gtaactgtag gcagttttta tgagcagttt ggtagcggaa tgatattgcg cagctatgaa
      541 gaacgtggcc ttggttatga taatgctttt gatggtgtgc gtgtaaaata caatcctact
      601 ccgggccttt atatgaaagg ttttattgcc cgccaacgtg cgtacttcga ttatgggccg
      661 ggcattgtac gtgggtttga tactgaatta aactttgccg aactgatgcc aaaggcgagt
      721 gaaaagggtt ggcaattaag ccttggtggt agctttgtaa gtaagtatca aaaagatgag
      781 gatgcattga aaaaattgcc cgaaaatgtt ggtgcttttt ctacaagagc aaatcttggg
      841 tacaaagggt ttacattcaa tgccgagtat gcctataaat acaacgaccc ttcggcagta
      901 aatactttta tttataaaga aggacaatct tttcttactt catttggtta tactaagaag
      961 ggattgggaa ttaatctcac cgcaaaacgc atcgataata tggattatcg cagcgaccgc
     1021 accgaaacgg tgagtacttt attaattaat tttatgccgg ctattgcgcg tcaatatact
     1081 tacaggttgc ttacacttta tccttatgca acacagccaa tgggcgaaat gggagccatg
     1141 gccgagttgt attatacctt caaacctaat acaggccttg gtggtaaaac tggaatgtac
     1201 atggccatca acagcacata tgtaaataat ttgaaagcta cacccgatac aaatggttat
     1261 ggatatacgt ccaatttttt agcaatagga aaggagcatt attatgcaga tgtaaatgta
     1321 gaattgacaa aaaaattatc gaaaaaggcc aagattactt tgaactatgt gtacatagaa
     1381 tataataaaa atgtgctcga aggcaaactg gattatggtg tgatttattc acatacagga
     1441 attttggatt ttacatacaa atttaatcat accaaaagta tccgcaccga gttgcagcat
     1501 ttgtatacaa agcaagacct tggtagttgg gcaatggcgt tagtagaatt ttctatggcg
     1561 ccacattggt cggtggctgc atttgccgag tacaattacg gcaacaaaaa acccgatgat
     1621 cggttttttt atccaaatgt ttcatcaacg tatgtgcata acagcaacag gttttcgcta
     1681 agttatggaa agcaacgtgc aggcttgtta tgtgttggag gagtttgtag atttgtacct
     1741 gcaagtaatg gcgtttcatt tacggtatca agtagttttt aatttataaa aatattaatc
     1801 atgacgaata aaattaaatt cagtttcttt tttgcgataa tggttctggc cactatcata
     1861 gcgagttgcg ataaggtgtc accacctttt gatgaaacag gaagcggagg caatcccaat
     1921 cctgatgtca ccgttcagaa agttttgata gaagatttta caggtcatac atgtaagaat
     1981 tgccctgctg catcaaaaat tgccgaaaat attcaaaaag cttatccggg tcgggtagtg
     2041 gtaatggcaa ttcactccgg cttttatgcc gatacaggcg caacaggaaa tttcacttac
     2101 aactttcgta ttcccgaagg gcaacaaata tcggatgact ttggcatttc ttcgtggccc
     2161 aatggcatgg tgaaccgcag aaaatataat ggctcaccta taacaggtaa agatagttgg
     2221 tcatcgcaca ccgggctgtg gttgcaagaa acacctaagg catcaattaa aataagtaat
     2281 gtgtataacg atgccacacg caacattacc actaccatta caagtaaagc nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta taataaaacc acaaatagta
     2401 gacaacacca ccgtgctcga ctatgtgcac atgcatgcat tgcgcggtgc tttcaatggt
     2461 gcctatggcg aagtggtgat gaataatcct gcaacaaacg attctatagt aaaaacatat
     2521 tcgctcacct tacgtgccga tgccgttgca aaaaactgcc atgtagtagc ctatataatt
     2581 gatgaaactt cgaaagaaat tattcaggta gaagagaagg aaatgaagtg agttggggaa
     2641 gtttaagagt tcaatagttc aaagttcaat agtttaggag ttcaatagtt caaagttcaa
     2701 t
//
LOCUS       DAORQA010000236         2686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8783_length_2686_cov_7.521475, whole genome shotgun sequence.
ACCESSION   DAORQA010000236 DAORQA010000000
VERSION     DAORQA010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8783_length_2686_cov_7.521475"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PK736_08525"
     CDS             complement(<1..307)
                     /locus_tag="PK736_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33472.1"
                     /translation="MKDKINTRYYTLFIFAVTSFVAAILLNGGYEDSQKNYSAKTKQF
                     QTILTSQEKKTLAFNTLISNNIEQQNFKNIDQCIPPLKLHHIDNKWYSILIFKNDSLV
                     "
     gene            complement(664..1287)
                     /locus_tag="PK736_08530"
     CDS             complement(664..1287)
                     /locus_tag="PK736_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33473.1"
                     /translation="MITQTMFCRFVLMLTLTVYATQGCNKKINPASSTKVETTNTSST
                     EKEKEPVLESSKNIKGSVMFTYDYCGGAAPSDEMIKRLRTPRAISNYTLYIKAGVKNN
                     DTPILDSSNCNEQGQFSFKVKPGKYVILTPDQIKTFSTKNYTSKYLQVDDEQCLKQWW
                     EDGLHKVNVANDNVTLADTILHQRCNGLGLVPCMSFTGPLPPMMRRE"
     gene            complement(1294..>2357)
                     /locus_tag="PK736_08535"
     CDS             complement(1294..>2357)
                     /locus_tag="PK736_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRC33474.1"
                     /translation="MEIYGKHVYPGLIGMNTNLGLSEIEAARATNDIYETGNINPSSR
                     SAIAYNTDSKIIPTVRSNGILIAQVVPSGGLVSGTSSVMQLDAWNWEDAVIKMDEGLH
                     INWPGMRIFKAWWAPTEEEQKDRMAKGLENIKKLLRESQSYCNEGAHQESNLNLQAMC
                     GLFNGTKKLYIHANYVKEIVAAVNVCKEYKIKMVLVGGADAWLIPQLLKENNIPVIIE
                     RTQTLPGREDDDIESSYRLPAMLQAAGVVYCISDAGFWQQRNLPFQAGNAVSYGITKE
                     QALESITLTPAKILGIEEQAGSLEQNKDATLIISDGDILDMKSNNIIAAYINGRKIDL
                     DNIQKQLYLKYCNKYGIKP"
     assembly_gap    2358..2367
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2368..2606)
                     /locus_tag="PK736_08540"
     CDS             complement(<2368..2606)
                     /locus_tag="PK736_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRC33475.1"
                     /translation="MNNKIKIIFILLTTITIYVAKAQNPAPSAFNQKSMLLRGGIAHI
                     GNGTVIENSLIGITKGKIDFVYDATTVKLQPMNYDT"
BASE COUNT          744 a          516 c          463 g          953 t
ORIGIN      
        1 aaaccaaact gtcgtttttg aaaattaata ttgagtacca tttgttatca atatgatgta
       61 gtttcagcgg gggaatacat tggtctatat tcttaaaatt ttgttgctca atattatttg
      121 atatcaaggt attaaaagca agtgtttttt tctcctgact tgttaatatt gtttgaaact
      181 gttttgtttt ggcgctataa tttttttgag agtcctcata tccaccattc aataagattg
      241 ctgccacaaa tgatgtaaca gcaaaaataa acagggtata atatcgggta ttgattttat
      301 ccttcatttt ttttggtgtg tcaggcatac ttaaaagttc aacagaaaaa acatttaatg
      361 cttcaccatt cttattattt aagattgagc tttgctaaaa attagttatg cgcaaggtaa
      421 ttgattttgg ataaacaatc attcttttta aagcaaacgg attaaatata accactttat
      481 tcaatatggc atcaattgct attattttct aaatatgaac aatcaacatc gtcagcccta
      541 aaatcctttt ttgtcaagca tatttattaa ttgcaattca tgcagtgaag catggcaatt
      601 gctataatgg taataaaagg atgccaccac tcaaaaaaac ctgcttgttt aactacctgg
      661 cttctactcc ctccgcatca taggaggcaa tgggccagtg aacgacatgc aaggaacaag
      721 ccccaaaccg ttgcagcgtt gatgcaaaat ggtgtcggcc aatgttacgt tatcatttgc
      781 aacattcact ttgtgcaatc cgtcttccca ccattgtttc aggcattgct catcgtcaac
      841 ctgcaaatat tttgaagtgt agttttttgt cgaaaaagtt ttgatttggt ctggagtaag
      901 aataacatat tttcccggtt ttactttaaa gctaaactgg ccttgctcgt tacaatttga
      961 actgtcaagt atgggagtat cgttattttt tactccggct tttatataca aagtatagtt
     1021 tgaaatagcc cggggtgtac gtagcctttt aatcatttca tcggaaggag cagcaccgcc
     1081 acaataatcg tacgtaaaca taacgctgcc ctttatattt tttgacgact ccaacaccgg
     1141 ctctttctct ttttcggttg aagatgtatt agtggtttct acttttgtgc tggatgctgg
     1201 attaattttt ttattacatc cttgtgtagc gtatacagtt agagttaaca ttaatacaaa
     1261 tctgcaaaac attgtttgtg ttatcatatt taattatggt tttatgccgt acttgttaca
     1321 atattttaag tacaactgtt tttgaatatt atctaaatct atttttcggc cgttaatata
     1381 tgcagcaata atattattgc ttttcatatc aagtatgtcg ccatcactaa ttatcaatgt
     1441 agcatcttta ttttgttcga gcgagcctgc ttgttcttca atacctaata tttttgcagg
     1501 agttaatgtg atggattcca atgcttgttc cttagtaata ccgtagctta cagcattgcc
     1561 tgcctggaat ggcaggttgc gctgttgcca aaaacctgca tcgctaatac aataaaccac
     1621 accggcagct tgcagcatgg ccggcaacct gtagctactt tcaatatcat catcctcgcg
     1681 accaggcaaa gtttgtgtac gttctattat tacaggtata ttgttttctt tcagcagttg
     1741 aggaataagc catgcatccg caccacccac cagtaccatt tttattttgt actctttaca
     1801 aacatttact gctgcaacta tttcctttac atagttagca tggatatata attttttggt
     1861 gccgttaaat aaaccacaca tagcttgcag gtttaaattc gattcctgat gtgcaccttc
     1921 gttgcaatat gattgtgatt cgcgcaaaag cttttttatg ttttcaaggc cttttgccat
     1981 gcggtctttt tgttcttctt cggtaggcgc ccaccatgct ttaaatattc gcatgcccgg
     2041 ccagtttata tgtagtcctt catccatttt tattacagca tcttcccagt tccaggcatc
     2101 gagctgcata accgatgagg tgccgcttac caatccccca ctgggaacca cttgcgctat
     2161 caatattcca ttgctgcgca cagtaggaat aattttcgaa tcggtattgt atgctatagc
     2221 cgatcgtgaa cttggattaa tatttccggt ttcataaata tcgttggtgg cgcgggcggc
     2281 ttctatttca ctcaatccca aattggtgtt catacctatc aatcccgggt atacatgctt
     2341 gccatatatt tccatcannn nnnnnnngta tcataattca tgggctgcaa cttcacagtt
     2401 gtagcatcat acacaaaatc tattttgcct tttgtaattc ctatgagcga attttcgata
     2461 acagtaccat tgccaatgtg cgctatgcca ccacgcaaaa gcatactctt ttgattgaaa
     2521 gccgaaggtg caggattttg tgcttttgct acgtatatag ttatagttgt aagcagtatg
     2581 aaaataattt taattttgtt gttcattgtt ttataataaa aattatggaa aattaatttt
     2641 gaaaaggcat atgctcaata tcggtacaat gtttttcttc atatgc
//
LOCUS       DAORQA010000237         2669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8854_length_2669_cov_10.425019, whole genome shotgun sequence.
ACCESSION   DAORQA010000237 DAORQA010000000
VERSION     DAORQA010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8854_length_2669_cov_10.425019"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..972)
                     /locus_tag="PK736_08545"
     CDS             complement(34..972)
                     /locus_tag="PK736_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRC33476.1"
                     /translation="MAVATKKIVKKILATIGEAKLNGSSTIGNRIPVVKTYKLYIDGK
                     FVRSESGRYMQINNKENFGANICRASRKDIRDAVVVARKAQSDWSKKTAYNKGQILYR
                     IGEMLEGRRAQLIHTMMLDGATETQSTNEINNAVDCFIYFAGWSDKYIQMFSSVNPVE
                     SAHFNFSYPEPTGVVTAICPDESPLFALANIIAPVIVGGNTIVVLSSQQYSRTACELA
                     EVLHSSDVSGGVVNILNGSKKELISNLASHKDVNAMIYVGTTGDENKIIETNCAINVK
                     RAIKLSATESNSNAGLHPYRIMSTQEIKTTWHPVGL"
     gene            complement(1002..2483)
                     /locus_tag="PK736_08550"
     CDS             complement(1002..2483)
                     /locus_tag="PK736_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRC33477.1"
                     /translation="MAKKNSSAGLNLAFDNGWQYAAAPESADHIKLKEKYDLFIDGKF
                     VAPTEGKYFATINPATEQKIAMVAEASQKDVDKAVRAANDAHINVWSKMPAKERGKYI
                     YRIARMIQERGRELAIIESMDGGKAIRESRDVDVPLAAAHFFYYAGWADKLQYAFAGR
                     NPKSLGVAGQIIPWNFPLLMAAWKIAPALACGNTVVLKPAETTSLTAMKLAEIIQDCD
                     LPPGVVNIISGAGATGAAIVNHPGINKIAFTGSTDVGKIIQRALAGTDKKYTLELGGK
                     AANIVFEDAAIDQAVEGIVNGIFFNQGHVCCAGSRLFVQENIKDTLLKKLKDRMDTLI
                     VGDPLDKNTDIGAINSKEQLKKIQNYIKIGTNEGGNCYQSNCVLPTKGFWCKPTLFMD
                     VAQSSRIVQEEIFGPVLAVQTFRTAEEAIEKANNTPFGLSSGVWTDKGSKIFKVTSKI
                     RAGVVWANTYNKFDPTSPFGGYKESGVGREGGMHGLDAYLKFN"
BASE COUNT          743 a          576 c          470 g          880 t
ORIGIN      
        1 aaccccaacc atatacgtac ctaagaacaa aaattataaa ccaactggat gccaggtagt
       61 ttttatttcc tgggtgctca taattcggta tgggtgtagg ccggcatttg aattactttc
      121 tgtggccgat aattttattg cacgcttaac atttattgca caattggttt ctataatttt
      181 attttcatca cccgtggtac cgacatagat catggcgttt acatccttgt gcgatgcgag
      241 attacttatc aattcttttt tgctgccatt caaaatattt actacaccgc cactcacatc
      301 agatgaatgc aatacttcgg caagttcgca tgccgtgcgc gaatattgtt gcgatgacaa
      361 taccactatt gtattgccgc caacaatcac cggagcaata atatttgcca gtgcaaacaa
      421 tggcgattca tcggggcaaa tagctgtaac aactccagta ggttcgggat acgagaaatt
      481 gaaatgagca ctctcaacag gatttacact gctaaacatt tgaatatatt tatcgctcca
      541 tccggcaaag tatataaagc aatcaacagc attatttatt tcgtttgttg attgagtttc
      601 tgttgcacca tcgagcatca tggtgtgtat gagttgtgct ctgcgtcctt caagcatttc
      661 gccaatacga tacaagatct gtcctttatt gtaagcagtt tttttactcc agtcgctttg
      721 cgccttgcgt gcaactacta cagcatcgcg aatgtctttg cgtgaagcac gacaaatgtt
      781 agcgccaaaa ttttctttgt tattgatctg catatatcgc cccgattcgc tgcgcacaaa
      841 cttaccatcg atgtataact tgtatgtttt tacaaccgga atacgatttc ctattgttga
      901 tgacccattc aattttgctt caccaatagt tgctaatatt ttctttacta ttttttttgt
      961 tgctactgcc atttttataa aactaaaatg atttaaaaaa attaattgaa ttttaaatat
     1021 gcatctaaac catgcatacc gccttcgcgg ccaacaccac tttctttgta accaccaaat
     1081 ggtgatgtag gatcgaattt gttatatgta tttgcccaca caacacccgc acgaattttt
     1141 gatgttactt taaatatttt cgacccttta tcggtccaca cgccactgct taatccgaag
     1201 ggagtgttat ttgctttttc gattgcttct tcggcagtgc gaaatgtttg tacggcaagc
     1261 acagggccaa atatttcttc ctgcactatt cggctcgact gtgctacatc cataaataaa
     1321 gttggcttac accagaaacc ttttgttggt aacacacaat tcgattgata gcaattgcca
     1381 ccttcgttag tgccaatttt aatgtaattc tgtatttttt ttaattgctc cttagagttg
     1441 atagcaccta tatctgtatt cttatccagt ggatcgccaa caattaaggt gtccatgcgg
     1501 tctttcaatt tttttagtaa agtgtcttta atattttcct gtacaaataa tcgcgacccg
     1561 gcacaacata catgaccctg attaaaaaat attccgttta caattccttc aactgcctga
     1621 tcaattgcag catcttcgaa tacaatattt gctgctttgc cgccaagttc aagcgtatat
     1681 tttttatcgg tgcctgccag tgccctttga attattttgc ctacatcggt cgaaccggta
     1741 aacgctattt tatttattcc cggatgattt acaatagcag cacctgtagc accggcaccc
     1801 gaaataatat ttaccacgcc cggtggcaaa tcgcaatcct gaataatttc ggcaagtttc
     1861 attgctgtaa gcgaagtggt ttcggcaggt tttaaaacaa cagtattacc acaagcaagg
     1921 gcaggtgcta ttttccaagc cgccataagc aaaggaaaat tccatggaat aatttgtcct
     1981 gcaacaccaa gcgatttggg attgcgtccg gcaaaagcat attgtaattt atcggcccag
     2041 ccggcatagt aaaaaaagtg agcagcagct aagggcacat caacatcgcg cgattcgcga
     2101 atggcttttc caccatccat actttctata attgccaatt cgcggccgcg ttcttgtatc
     2161 attctggcaa tgcgataaat atatttgcca cgttctttag caggcatctt gctccacaca
     2221 ttaatatgtg catcgtttgc agctctcacc gctttgtcca catccttttg tgatgcttcg
     2281 gctaccatcg ctattttttg ttctgttgcc ggatttatgg ttgcaaaata ttttccttcg
     2341 gttggagcaa caaattttcc atcgataaag agatcgtact tttcttttag cttaatatgg
     2401 tcggcacttt cgggggccgc tgcatattgc catccattat caaatgctaa attcaatcct
     2461 gcggagctgt tcttttttgc cattatattt atctttgaat aagcgaaagt aataaaaaac
     2521 ttttgtatca ttcttgtgaa aaaattttaa gtgctcacaa gggaaagtaa tttcagaaac
     2581 aaacattaat gcgttaacat aatgggacac tttattcaaa aaaataagtt ccattatcaa
     2641 gaaggttttt tttcaaaata aaaattaag
//
LOCUS       DAORQA010000238         2658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8895_length_2658_cov_17.568575, whole genome shotgun sequence.
ACCESSION   DAORQA010000238 DAORQA010000000
VERSION     DAORQA010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8895_length_2658_cov_17.568575"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..500
                     /gene="ytxJ"
                     /locus_tag="PK736_08555"
     CDS             162..500
                     /gene="ytxJ"
                     /locus_tag="PK736_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HRC33478.1"
                     /translation="MINWKSLTTKEDISEAIKSSFDENSVTCIFKHSTRCSVSRMVLK
                     QFEAEALPEVNGNFYFLDLLSYREISDAIACEFSVQHQSPQLLILKNGKAIHAESHEN
                     ISFNNILINN"
     gene            514..948
                     /locus_tag="PK736_08560"
     CDS             514..948
                     /locus_tag="PK736_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33479.1"
                     /translation="MKKILILFFAMAMLQIVANGQAKKKPVAKIKAPTKCGFFMDGKM
                     ANKVTAEQMIAWCDQEKLYVMCDNGKKYLLKSFEISVIQKEPYAMKDFGIGEGGMPIM
                     ARREIKNIKEGDSLFLKNLLGLDDNMLEVKLPVLVVGVYNQK"
     gene            1012..>2658
                     /locus_tag="PK736_08565"
     CDS             1012..>2658
                     /locus_tag="PK736_08565"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HRC33480.1"
                     /translation="MKNYSVILLSIFLGIKVNAQNAHIVVSDSKANTALLEYSAGKIV
                     PCYITFKDNYNLAPQMAFIELSDELSIRNEDTWKILRSEQDDIGYTHHRYQQYYKGIK
                     VEQGEFIIHEKNNRTVSANGDFIDNIDLSIIPGVTRTAALNFALADINATTYKWQFAG
                     EENWYKQIMNNPKATLYPKAELVIIRLNNNIATKEKQRLAWKFDIYTHDPNGRWFVYV
                     DANTGDILDKVDRICEFVAPGIANTKYSGVQPIVSDSLGPNNFRLRDYSRGAPIATYD
                     MNKGTSYGSAVDFTDDNNVWDSITNQDNAAYDAHHGAEKTWDYFNSVHGRNSYDNFGS
                     PLISYIHYSNNYNNAFWNGSVMTYGDGNGSTFSPLTTIDICGHELTHGVTGNTSNLVY
                     SYESGALNESFSDIFGTCIDFFAFPGSANWKLGEQCYTPGTSGDALRHLDNPNAASDP
                     DTYLGTFWWTSSGDNGGVHTNSGVQNFWFYLLSQGGTGTNDVGFNYNVSAIGMANARL
                     IAYRNNAYYLTSGSVYADARRYSIIAAADIFGPCSNEMIQT"
BASE COUNT          857 a          452 c          553 g          796 t
ORIGIN      
        1 aagttcctca tcgggctatt taacaatgcc tttttacact tctgttgtta cagttcaagg
       61 tggtggattt gctgacgaga aagaattttt ggcttttctt gaattatttg attggaattt
      121 acttgccaag aatatgggat atataaaaga gtaattcatt aatgataaac tggaaatcgc
      181 tcaccacaaa agaagatata tccgaagcca taaaaagcag ttttgacgag aatagtgtaa
      241 cctgtatttt taagcacagc acacgttgca gtgtgagtcg catggtatta aagcagtttg
      301 aagcagaagc gttgccggag gtgaatggca atttttattt tcttgatctt ctcagctacc
      361 gcgaaatttc ggatgccatt gcatgcgaat tttctgtaca acatcaatca ccacaacttt
      421 taattttaaa aaacggtaaa gcgattcatg ctgagtcgca tgagaatatt tcgttcaata
      481 atattttaat aaataattaa acaaaagatg actatgaaga aaatattgat tttatttttt
      541 gcaatggcga tgttgcaaat tgtagcaaat ggacaagcaa aaaagaaacc cgttgccaaa
      601 ataaaagcac caacaaaatg cggtttcttt atggatggta aaatggcaaa taaagttact
      661 gccgagcaaa tgatagcctg gtgcgatcag gaaaaacttt atgtaatgtg cgataacggt
      721 aaaaaatatt tacttaaaag ttttgaaata agtgttattc aaaaggaacc ttacgctatg
      781 aaggattttg gaattggcga aggcggtatg cctattatgg cgcgtagaga aataaagaat
      841 attaaagaag gcgattcgct ttttcttaaa aatttacttg gccttgacga caatatgctc
      901 gaagtaaaac tacctgtact tgtggtaggt gtttacaatc aaaaatagtt ttataaaaag
      961 cacagttttt gcatctgtag ctataatcac aaattattta ttaaaatgaa tatgaaaaat
     1021 tactcagtta ttttgttgtc aatttttttg ggaataaaag taaatgcaca aaatgcgcac
     1081 atagtggtga gcgattcgaa agcaaatacc gctttgcttg aatattctgc aggtaagatt
     1141 gttccgtgtt acattacctt taaagacaat tacaaccttg caccacaaat ggctttcatt
     1201 gaacttagtg atgaattgag tattcgcaac gaggatacct ggaaaatact gcgttcggaa
     1261 caggacgata ttggatatac gcatcaccgg tatcaacaat attacaaagg aataaaagta
     1321 gagcagggcg aatttatcat tcacgaaaaa aataatagaa cagtatcggc taatggcgat
     1381 tttattgaca atatcgattt aagtattatt ccgggagtca cccgcacggc agcattaaat
     1441 tttgctttag ctgatatcaa tgccacaact tataaatggc aatttgccgg tgaagaaaac
     1501 tggtacaagc aaattatgaa taatcccaaa gctactttgt acccaaaggc agaattggtt
     1561 attataagac tcaacaataa tattgctact aaagaaaagc agcgtttggc gtggaagttt
     1621 gatatttaca cgcatgatcc caatggccgc tggtttgttt atgtagatgc aaacaccgga
     1681 gatattttgg acaaagtgga caggatatgt gaatttgttg cacccggtat tgccaatacg
     1741 aaatatagcg gagtacaacc aatagtttct gatagccttg ggccaaataa ctttcgcttg
     1801 cgcgattaca gccgcggtgc gcctattgca acatacgaca tgaataaagg aacgagctat
     1861 gggagtgctg tagattttac tgacgacaac aatgtgtggg actctatcac caaccaggat
     1921 aatgctgcat atgatgctca ccatggtgcc gaaaagacat gggattattt caattcggtg
     1981 catggtcgca acagttatga taattttggc tctccattaa tttcttacat tcattattca
     2041 aacaactata acaatgcttt ttggaatggt agcgtaatga cttatggtga tggcaatggt
     2101 tctacttttt caccgctgac tactattgat atttgtggac atgaattaac acatggcgtt
     2161 acaggtaata cttccaatct tgtgtattct tatgagtcgg gtgccctaaa cgaatcattc
     2221 agcgacattt ttggaacatg catcgatttc tttgcatttc cgggaagtgc aaactggaaa
     2281 ttgggagaac aatgctatac tcccggcaca agtggcgatg ccttgcggca ccttgataac
     2341 cccaatgcag ccagcgatcc cgacacatat ttgggaacat tttggtggac cagttcaggc
     2401 gataacggtg gggtgcatac gaatagcgga gttcagaatt tttggtttta tttattaagc
     2461 cagggtggaa ccggtaccaa tgatgtaggt tttaattaca atgtaagcgc tataggaatg
     2521 gccaatgcac gtttaatcgc atataggaat aatgcttatt atttaacttc gggctctgtt
     2581 tatgccgatg ctcgcagata ttcgattata gccgctgccg atatttttgg cccttgcagc
     2641 aatgagatga ttcaaact
//
LOCUS       DAORQA010000239         2653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8922_length_2653_cov_5.107775, whole genome shotgun sequence.
ACCESSION   DAORQA010000239 DAORQA010000000
VERSION     DAORQA010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8922_length_2653_cov_5.107775"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..423
                     /locus_tag="PK736_08570"
                     /pseudo
     CDS             1..423
                     /locus_tag="PK736_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095271.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
     gene            546..2168
                     /locus_tag="PK736_08575"
     CDS             546..2168
                     /locus_tag="PK736_08575"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRC33481.1"
                     /translation="MKVQFYYLVTLFFIVACTFAMSQNNIIETIVDTDPRIIKSFTLK
                     DLQPNQFYLAAPFAKAMLLRNPEMDKIKDFAIYKVELVYTTYKESKNFDQRELNINRL
                     KNLKKTYPEIFENILAEWSQIAIDGATTRQEGKEMFHGFIITYRLPPSKEKTETEIGE
                     IEKILAGIKTKKDFEADSIMKLIKKPKKTLPVYEMPRFIGGNDSLQKYIDRKIKYPKK
                     ALQDKKQGLVKIAFKIDSRGKVVSPYLVSGIESKCNDVALDLFDKMPNWIPGKRDGKP
                     VVYEFQIALRFDADKNKIYCDTLKFEGASKDNKGGGKYEDISEMIAKEQEEEISDWMR
                     EQDSIVFNSLIRNNFGEALLLCDVTGSMSPYTGQTLLCIKNNIAKNNIKQIVFFNDGD
                     NMLDYEKKMGEVGGIYITDKINIDTIIQLAYTAMRNGNGGDNPENNIEAALAGTAKYP
                     ATKSIIMIADNYATPRDMELLSQLRVPVHVILCGASHFINPNYLAIARRTKGSLHTMQ
                     HDLLNLDKMQEMETIKINGETYRLVRGKFMRL"
BASE COUNT          956 a          436 c          489 g          772 t
ORIGIN      
        1 tatggggatg gagttatggg ggttacatga cttcgttgtg tatggccaag ggtgccgatg
       61 tattcaaggc cggtattgca gttgctccgg taacaaattg gcggtattac gattccattt
      121 atacagaaag atacttgcgc actccacaag aaaatgcttc ggggtacgat gataattcgc
      181 caataaattt tgccgaaaaa attaaaggta aattcctgct ggttcatggt gtagcggacg
      241 acaatgttca ttattcaaaa ctcgatgatg tttgtaaata aattgataaa aaacaatatt
      301 caattcgaaa ccatgaatta cccaaacaga aatcatggta ttagtggcgg tggggcaaga
      361 ctgcacttgt atacgatgat gacaaagttt attgaagatt atttattatc aatatcgcta
      421 taggcgataa gattgatagc cctattctgc ttttttttcg ttttggctga tataaaattg
      481 taagcatttg tcgccttaat taagtctaca ataaaacact tttcctatcc gtttataact
      541 aactgatgaa agtacaattc tactatttgg tcactttatt ttttattgtt gcatgcacat
      601 ttgcaatgtc gcaaaataac attatagaaa caatagtaga taccgacccg cgcattataa
      661 aatcattcac cttgaaagat ttacaaccaa atcaatttta tttggccgca ccatttgcaa
      721 aagcaatgtt attgcgcaat cctgaaatgg acaagataaa ggattttgct atttataaag
      781 tagaattggt gtacactact tataaagaaa gtaaaaactt cgaccagcga gaattgaaca
      841 taaaccgttt aaaaaattta aaaaaaacat accccgaaat atttgaaaac atacttgcag
      901 agtggagtca aattgcaatt gatggagcaa caacccggca agaaggaaaa gaaatgtttc
      961 atggatttat tatcacatac cgattgccac cgagcaaaga aaaaaccgaa accgaaatag
     1021 gagaaataga aaaaattctt gccggcataa aaacgaaaaa agattttgaa gctgattcga
     1081 taatgaagct gataaaaaag ccaaaaaaaa cattaccggt ttatgaaatg ccaaggttta
     1141 ttggcggtaa cgattcgttg caaaagtaca ttgacaggaa aataaaatat cctaaaaaag
     1201 cattacaaga taaaaaacaa gggctggtga aaattgcctt caaaattgat tctcgtggca
     1261 aggttgtttc gccatattta gtgagtggta tagaaagcaa atgcaatgat gttgcgctgg
     1321 atttatttga caaaatgccc aactggattc caggcaaacg cgatggaaaa ccggtagtat
     1381 acgaatttca aattgcattg agatttgatg ctgataaaaa caaaatttat tgcgacacat
     1441 taaagtttga aggtgcttcg aaagataaca aagggggtgg caaatacgaa gatatatcag
     1501 aaatgattgc caaagaacaa gaagaagaaa taagtgattg gatgcgagaa caagattcaa
     1561 ttgttttcaa ttcgttgata agaaataatt ttggtgaagc tttgttgttg tgcgatgtta
     1621 caggaagcat gtcaccatac actggtcaaa ctttgctttg cattaaaaac aatattgcca
     1681 aaaataacat taagcaaata gtctttttta atgatggtga caatatgctt gactatgaaa
     1741 aaaaaatggg tgaagttggc ggcatttata ttactgataa aataaacatc gataccatta
     1801 tccaacttgc atacactgcc atgcgaaatg gaaatggtgg cgacaaccct gaaaacaata
     1861 ttgaagcagc gcttgcaggc acggcaaaat atccggcaac aaaaagcatt attatgattg
     1921 ccgacaacta tgcaacacca cgcgatatgg aattgctatc gcaactgagg gttccggtac
     1981 atgttatttt atgtggcgca tcacatttta taaaccccaa ctatttagca attgcccgaa
     2041 ggacaaaagg ctccttacat acaatgcaac acgacttact caacctcgat aaaatgcaag
     2101 aaatggaaac tattaaaatt aatggcgaaa catacaggct ggttcgaggt aaatttatga
     2161 ggctataatt ttcccttttt tacaaaagct ttctcgacat atttttttag tgataaaaca
     2221 ttcactatta aactaaagat attgacaaca aacaattaat attgccgatg taaaataaat
     2281 cgaaaacaat catgcatttt gttctggcaa tattagtgtt taccatacta ctactatgca
     2341 tgttgatgta tcggcttatt ttttattttc aatttgatat ctcaagccaa atacatgacg
     2401 cgaatatatg gtggcaatca ataatcacag gtgtacgatt tgatattgtt actgctgcat
     2461 atttaaccat tttaccatta ctttttattt taatccattg ttggttaaat ccgaaaagaa
     2521 aagcaattgc cataagtaca aaaattgtat tgagcataat tgccataact tgttcgctca
     2581 tcatggcagc cgattacgca ttttttaggc agtaccataa tcgcattaca aaggcgattc
     2641 acttatggaa cga
//
LOCUS       DAORQA010000240         2648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8938_length_2648_cov_6.283455, whole genome shotgun sequence.
ACCESSION   DAORQA010000240 DAORQA010000000
VERSION     DAORQA010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8938_length_2648_cov_6.283455"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PK736_08580"
     CDS             <1..503
                     /locus_tag="PK736_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRC33482.1"
                     /translation="YKMPPSVYTLTRAIACYERSLFSGESRYDEYLYEKNTAALTPSE
                     INGMNIFFGEQGECFHCHGEFNFTDYSFKNNGLYLNYADSGRARITALPSDVGKFKVP
                     SLRNIELTAPYMHDGSLATLTDVIEHYNSGGKAHPNKSGLIQPLNLSAQEKQDIINFL
                     NALTDK"
     gene            complement(647..>1452)
                     /locus_tag="PK736_08585"
     CDS             complement(647..>1452)
                     /locus_tag="PK736_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRC33483.1"
                     /translation="FVGSEGTLGIITKIVVRLIPLPKHDLLMLVPFASAEKACEAVAA
                     VFKAGITPSAMEFMERDAIDFTMKYIQTSMVIKDHIKAHLLIEVDGNYEDVLMQDCEK
                     ISEIMYAFDCDDVLFAQSSAEKANLWKLRRSVGEAVKSHSIYKEEDTVVPRAHLPVLL
                     RGVKSIGEKYGFKSVCYGHAGDGNLHVNIIKDNLSDEEWNKKLPLGIREIFELCVSLR
                     GTISGEHGIGYVQKSYMDIAFNKIEIELQKNIKTVFDPHHILNPGKMFL"
     assembly_gap    1453..1467
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1468..1983)
                     /locus_tag="PK736_08590"
     CDS             complement(<1468..1983)
                     /locus_tag="PK736_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRC33484.1"
                     /translation="MNDAESLAHYAHDYTEDLHFEPQLVLRPKTVEQVQNIMAYCHQH
                     HIAVTPRAAGTGLSGGALPVHGGILLSVERMNRIIEIDENNLQATIEPGVINQVLRNE
                     VESKGLFYPPDPASKGSCMMGGNVAENSGGPKAVKYGVTKDYILNLEVVLPTGELIWT
                     GANVLKNSTGYK"
     gene            complement(2062..>2648)
                     /locus_tag="PK736_08595"
     CDS             complement(2062..>2648)
                     /locus_tag="PK736_08595"
                     /inference="COORDINATES: protein motif:HMM:NF027450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family zinc-binding domain-containing
                     protein"
                     /protein_id="HRC33485.1"
                     /translation="FIKVLLRSYEGLFDEYVSIYESEIAKRSNTTIEKVVQRLNYLNK
                     LQVLDYIPFTDKPQLTLLRERLPIANVDISTETLANRKKRYVMRLSAIENYVTSHEHC
                     RNMLLLKYFDEIKTERCGTCDYCRERNKLELKDKILDDLKGRVISLIEATPLTVEEIE
                     QKVFTVSKNKIVAIMRWLLETNAVKLIENKYYLK"
BASE COUNT          760 a          551 c          474 g          848 t
ORIGIN      
        1 cttataaaat gccacctagc gtatacacac ttacgcgtgc cattgcatgt tatgagcgtt
       61 cacttttttc gggcgaatcg cgctacgatg aatacctgta tgaaaaaaat accgcagcat
      121 taacaccatc ggaaataaat ggtatgaata ttttttttgg tgagcaaggg gaatgttttc
      181 attgtcatgg agaatttaat tttaccgatt acagttttaa aaacaacggc ctttatctta
      241 actatgccga tagcggcagg gcaagaatta cggccctacc atccgatgtt ggcaaattta
      301 aagtgccctc gcttcgcaat attgagctta ccgcaccata tatgcacgat ggttcgttag
      361 caacgctaac cgatgttata gaacattata atagtggtgg taaggcacat ccaaataaga
      421 gcggattaat acagccgttg aatttaagtg cgcaagagaa gcaagatatt attaattttc
      481 tcaatgcatt gacagacaaa taggcgcgtt gacaaaaaaa tcctcttaaa atcaaaagat
      541 ttttttttgc cttttggtac aaggcgagtt aaaggcaaaa ttgttttttg cactgctttt
      601 cgatattgct tttgggtttt actgcaaatc gatttaatga gaactgttac aaaaacattt
      661 tacccggatt taaaatgtga tgtggatcga aaactgtttt tatgtttttc tgcagttcga
      721 tttcgatttt attgaaagca atgtccatgt aagatttttg cacatagcct attccatgct
      781 cgcccgaaat ggtaccgcgc aacgatacac ataactcaaa tatttcgcga atgcctaatg
      841 gcaatttctt attccactct tcatcgctga gattatcttt tataatattt acatgtagat
      901 tgccatcgcc tgcatggcca tagcaaacac ttttgaaacc atatttttcg ccaatcgatt
      961 ttacaccgcg aagcaaaaca ggaagatgtg cacgtggcac cacagtatct tcttctttat
     1021 aaatggagtg tgattttacg gcctcgccaa ctgacctgcg caatttccac aggttggctt
     1081 tttcggccga tgattgcgca aataaaacat catcgcaatc gaaggcatac attatttccg
     1141 atattttttc gcaatcctgc atcagcacat cttcgtaatt gccatctact tcaataagta
     1201 aatgtgcttt gatatgatct ttaattacca tgcttgtttg aatatacttc atggtaaaat
     1261 caatagcatc gcgttccata aattccatgg ccgatggagt aatgcctgct ttaaaaaccg
     1321 cagccacggc ctcacacgcc ttttcggccg aagcaaaagg aacaagcata agcaaatcgt
     1381 gtttcggtaa cggaattaaa cgcactacaa tttttgtaat tattcctagc gtgccttcgc
     1441 tgccaacaaa cannnnnnnn nnnnnnnttt gtatcctgta gaatttttta aaacgtttgc
     1501 acctgtccat attaattctc cggtaggtaa taccacttca agatttaaaa tataatcttt
     1561 agtaacgcca tacttcacag ctttgggtcc gcccgaattt tcggcaacat taccacccat
     1621 catgcacgag cctttacttg caggatcggg tggataaaac aatcctttac tctccacctc
     1681 gttgcgcaat acctgattga taacacccgg ttcgatggtt gcctgcaaat tattttcatc
     1741 aatttctata atgcgattca tacgctctac cgacaataaa attccaccat gcacaggcag
     1801 tgcacctccg cttaggcctg tgcctgcagc gcgtggagtt actgcaatat gatgttgatg
     1861 gcaatatgcc attatgtttt gcacttgctc cactgttttg ggacgcaata caagttgtgg
     1921 ttcgaaatgt aaatcttcgg tatagtcatg tgcataatgc gcgagcgatt cggcatcgtt
     1981 catcacatgt tctgtggcta atagtgttaa aaaaaattgc cggtcggcag tatcaatatc
     2041 cttatacatt gtatagaaat tttattttaa ataatatttg ttctcaatta atttcacggc
     2101 atttgtttca agtagccagc gcattattgc aacgatttta tttttcgaaa cagtaaaaac
     2161 cttttgttct atttcttcaa cggtcaaagg tgttgcttca attaaagaaa ttactctgcc
     2221 ttttaagtca tcaagaattt tatctttcaa ctcaagttta tttctttcgc ggcaataatc
     2281 gcaagtgccg caccgctcgg tttttatttc atcgaaatat tttagcaaca acatattacg
     2341 acaatgctcg tgcgaagtaa cgtagttttc tattgccgac aatcgcataa catatctttt
     2401 tttgcggtta gccaatgttt cggtgctgat gtcaacattg gctattggca accgttcgcg
     2461 cagcaaagta agttgaggct tatcggtaaa cgggatataa tccaacactt gaagtttgtt
     2521 taaatagttt agtcgctgca ccactttttc gattgttgta ttactacgct ttgctatttc
     2581 actttcgtaa atagaaacat actcgtcaaa caatccttcg tacgaacgta acagcacttt
     2641 tataaaac
//
LOCUS       DAORQA010000241         2642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8954_length_2642_cov_34.175493, whole genome shotgun sequence.
ACCESSION   DAORQA010000241 DAORQA010000000
VERSION     DAORQA010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8954_length_2642_cov_34.175493"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="PK736_08600"
     CDS             <1..1540
                     /locus_tag="PK736_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33486.1"
                     /translation="CGDYLNNGTLTAAATSTVIFNNGSVNQSMDGNLVGASAFGNVTI
                     TKTGGTVTTLDNVEMRGNFTVSNATSSFNANAKYHKVGGHFINSGTYTPGVAGTLELN
                     GSATQDYQNTGAINNCIINKTSNIVNLLSNMLLGNLGNLTLTWGNINTSPSYMVIVTN
                     RNVGSCTPGHNNSFVAGFLRRYINPTGSYDFPVGSIAKSYQRANVNFAYPGNPTIIDY
                     LTASFYAFTVLPPGIGGNECSTSYTQPALNNGYWTISASNNPTSGNYDMTLYNQAFTN
                     ASSGWTVMKNPGGGWGLYNGTCVICTPNAVMRLGMNGFSDFATAQSPSPLPLQLLQFE
                     AFGKLNQVNVYWTTTSEKEVSHFELERSLNETDFEKIASIDAHNNPAAINHYMYEDKN
                     VSSNVVYYYRLKQMDVNGFYKYSDIKTAELNENNDVFTLSPNPYTKSTSVQYYLDFEA
                     DITIDVIDPTGNIVKNIVHQNQQKGFYMFNLNLHEVTTAEGIYTVRFSNNGKVWSKKL
                     VYKW"
     gene            1678..1923
                     /locus_tag="PK736_08605"
     CDS             1678..1923
                     /locus_tag="PK736_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33487.1"
                     /translation="MKKIFVIFCFLSLFTINESNAQCAMCKATAESNMKDGKSTVGRG
                     LNSGILFLMAIPYMMAATGFGIWYYNKKKRQKLVLAE"
     gene            complement(1976..2311)
                     /locus_tag="PK736_08610"
     CDS             complement(1976..2311)
                     /locus_tag="PK736_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRC33488.1"
                     /translation="MPYKEKEIEKIFFSIGEVAALFNVNPSLIRFYEKEFDILRPAKT
                     STGNRQFTKTDIRNFELIFHLVREKGFTIDGAKNFLKTNNQSERKKLMLRESLLDLKK
                     FLQELSSKI"
     gene            complement(2322..>2642)
                     /locus_tag="PK736_08615"
     CDS             complement(2322..>2642)
                     /locus_tag="PK736_08615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRC33489.1"
                     /translation="GDGVVERIEFDGRGYGNHVVIRHGYGYETLYGHMSKILVRSGMK
                     IKRGDRIGLVGSTGASTAPHCHYEVMKDGKKIDPVNFFYNDLTPQQYQMVIELASQAN
                     QSFD"
BASE COUNT          797 a          524 c          507 g          814 t
ORIGIN      
        1 gtgtggcgac tacctcaaca acggaacgct gacagctgcg gcaacttcaa cagttatttt
       61 caataatggc tctgtcaatc aatcgatgga tggtaaccta gtaggtgcaa gtgcttttgg
      121 caatgtaact attactaaaa caggtggtac tgttactacg cttgataacg ttgaaatgcg
      181 cggcaatttt acggtgagca atgctaccag ttcattcaat gccaatgcaa agtatcataa
      241 agttggtggt cattttataa atagtggaac ttacactccg ggtgtggccg gaacattaga
      301 attgaatggc agtgcaactc aggattacca aaacacaggc gcaataaata attgcataat
      361 caacaagacg agcaacattg taaacctgct atcaaatatg ttgctgggca atttgggtaa
      421 cctcacttta acatggggaa atattaacac atctccatcg tatatggtta tagttacaaa
      481 ccgcaatgtg ggctcatgca ctcccgggca taacaacagt tttgttgccg gatttttgcg
      541 cagatatatt aaccctaccg gaagttatga ttttcctgtt ggaagtatcg ccaaaagcta
      601 tcagcgggca aatgtaaatt ttgcatatcc cggcaaccca accatcatcg attatcttac
      661 agcaagcttt tatgccttta ccgttttgcc tccgggaatt ggtggcaacg agtgcagcac
      721 atcgtataca cagcctgcgc ttaacaatgg ctattggacc atcagtgcaa gtaacaatcc
      781 tacatcgggc aattatgata tgactttata caatcaggca tttaccaatg cttcatcggg
      841 ctggacggta atgaaaaacc ccggaggtgg atggggatta tataatggca cctgcgttat
      901 atgcacaccc aatgcagtaa tgcgtttggg tatgaatggc ttttcggatt ttgccaccgc
      961 acaatcacca tcgccattac ccttgcaatt attgcagttt gaggcgtttg gaaaattgaa
     1021 tcaggtaaat gtctattgga ccactacttc cgaaaaagag gtaagtcatt ttgagttaga
     1081 acgtagccta aatgaaaccg atttcgaaaa aattgcctca atagacgctc acaataatcc
     1141 ggcagcaata aaccattata tgtatgaaga taaaaatgta agttccaatg tggtttacta
     1201 ttatcgatta aagcaaatgg atgtgaacgg tttttacaag tattcggata ttaaaactgc
     1261 agagttaaat gaaaataacg atgtttttac attgtcgcca aacccttata caaaaagcac
     1321 ctcggtgcaa tactatctcg attttgaagc cgatattact attgatgtaa tagaccctac
     1381 aggaaatatt gtaaaaaaca ttgtacacca aaatcagcaa aagggttttt acatgttcaa
     1441 cctcaatttg cacgaagtta caacggcaga agggatttat accgtgcggt ttagcaataa
     1501 cggtaaagta tggagtaaga agttggtgta caaatggtaa tccattttaa tgcttcggtt
     1561 tcatttttgt cgtttgttat tgaagcgttt taaaaagcta tcagcttgat acttcttaaa
     1621 aaaaaacttt aatatgcatg gtttgtttta attgacagca tcatttacct tcgcaccatg
     1681 aaaaagatat tcgttatatt ttgttttctt tcgcttttta caattaatga aagtaatgcg
     1741 cagtgtgcca tgtgtaaggc tactgccgaa tcgaacatga aagacggcaa aagcactgtg
     1801 ggccgtggac ttaacagtgg cattttgttc ctgatggcca ttccatacat gatggctgct
     1861 accggtttcg gtatatggta ttacaacaaa aagaagagac agaaattagt tttggcagaa
     1921 taatttcgtt gcgaatagac ttttatacca tagcgaataa cctaatggtc atctcttaaa
     1981 ttttactgct cagctcctgc aaaaattttt tcaaatcaag taacgactcc ctcagcataa
     2041 gttttttgcg ttcgctttga ttgttggttt tcaaaaaatt ttttgcccca tcaatggtaa
     2101 atcctttttc acgtacgaga tgaaaaatga gttcaaaatt gcggatatca gtctttgtaa
     2161 attgtcggtt tccggttgat gtttttgccg gacgaaggat atcaaactcc ttttcataaa
     2221 aacgtattag tgaaggattc acattgaaaa gcgcggccac ttctcctatc gaaaaaaata
     2281 tcttttctat ctccttttcc ttatacggca tgttttactg tttaatcgaa cgattgattt
     2341 gcctgacttg caagttcaat aaccatttga tattgttgtg gtgttaaatc gttataaaag
     2401 aaatttaccg ggtctatttt ttttccatct ttcatcactt cgtagtggca atgcggtgca
     2461 gttgaggcgc ctgtacttcc taccaaacct atacgatcgc cccgctttat tttcattccc
     2521 gaacgcacca gtattttact catatgtcca tacaaagttt cgtaaccgta gccatgtctt
     2581 atcactacat ggttaccata tccacggcca tcaaactcaa ttctttctac taccccatcg
     2641 cc
//
LOCUS       DAORQA010000242         2634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8984_length_2634_cov_7.377278, whole genome shotgun sequence.
ACCESSION   DAORQA010000242 DAORQA010000000
VERSION     DAORQA010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8984_length_2634_cov_7.377278"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..2225
                     /gene="recQ"
                     /locus_tag="PK736_08620"
     CDS             6..2225
                     /gene="recQ"
                     /locus_tag="PK736_08620"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRC33490.1"
                     /translation="MPSGLYIELSSKELHQLLNKYFGFDKFKGEQEEIIQNLLQGKDS
                     FVIMPTGGGKSMCYQLPALITPGTAIIVSPLIALMKNQVDAIRGFNTDDAIAHFLNSS
                     LSKTEIAQVRKDIKSGVTKLLYVAPESLTKEENVEFFKDIDISFFAIDEAHCISEWGH
                     DFRPEYRRLRPIMDAIGEKIPVIALTATATAKVQQDIVKNLGIPEARLFKASFNRANL
                     YYEVRPKQDVIKEIIKYVKNHANKSGIIYCLSRKKVEEVAETLSVNGIRALPYHAGLE
                     AAMRADHQDKFLMEKYDIIVATIAFGMGIDKPDVRFVIHHDIPKSLEGYYQETGRAGR
                     DGGEGNCITFYSYKDIEKLEKFMKGKPVAEQEIGKQLLLETVSFAESSVCRRKMLLHY
                     FGEEYTQENCGNCDNCLHPKSKIEAQDEVITMLETVLAVKEKHVAEHVINVIMGVSEK
                     SVKDYGHDKLDCFADGADYDARFWMAVIRQALVAGFMYKEVENYGLLKLTDKGKKFIA
                     SPHSFMITKDHEYNNTDEEEDDEMGGGGAGGGGGADPQMFSMLKDLRKKIAKQKNVPP
                     FVVFQDPSLEEMSIQYPITIDELKNITGVGAGKAVKYGKPFIELIAKYVEENEIERAV
                     DMVVKSVVNKSGLKVYIIQNIDRKIALDDMAESKGITMSDLITEIESIVGSGTKIDIN
                     YYLNEVIDIDKQQEVFDFFRAAETDSVRDALTELGEDEYTEEEIRLMRIRFMSELGN"
BASE COUNT          881 a          468 c          574 g          711 t
ORIGIN      
        1 aaattatgcc gagtgggtta tacatcgaat tatcgtcaaa agagttgcat caacttttga
       61 ataaatattt tggattcgat aagtttaaag gcgagcaaga agaaattatt cagaatttgt
      121 tgcagggcaa agattcattt gtgattatgc ccacaggtgg tggtaaaagc atgtgctatc
      181 agttaccggc acttatcact cccggcacag ccatcattgt atcgccactc atagcactga
      241 tgaaaaatca ggtagatgcc attcgcggat tcaacaccga tgatgccata gcacattttt
      301 taaattcatc gttatcgaaa accgaaatag cacaagtgcg gaaggacatt aaaagtggtg
      361 ttaccaaact attatatgtt gcacccgagt cgttaaccaa agaagaaaat gtagaatttt
      421 ttaaagacat cgatatctca ttttttgcca ttgatgaagc acattgtata tcggagtggg
      481 gtcacgattt ccgccccgag tatcgcaggt tgcgtcctat aatggatgcc ataggcgaaa
      541 aaattcctgt aattgcttta accgccactg ccactgcaaa agtgcagcag gacattgtaa
      601 aaaatcttgg cattcccgag gcccgtttat tcaaagcatc gtttaaccgt gctaacttat
      661 attacgaagt acgacctaag caagatgtaa taaaggagat aataaagtat gtaaaaaacc
      721 acgctaataa atcgggaatt atctattgcc tcagccgtaa aaaagtagaa gaagtagccg
      781 aaacattaag cgtaaatgga atacgtgccc tcccctatca tgcaggcctc gaagccgcta
      841 tgcgtgccga ccatcaggat aaattcctca tggaaaagta cgacattatt gttgcgacta
      901 ttgcctttgg aatgggaata gataaacccg atgtacggtt tgtaattcac cacgacattc
      961 caaaatcgct cgaaggatat tatcaggaaa caggacgtgc cggtcgcgat ggtggcgaag
     1021 gaaattgcat tactttttat tcttacaaag atattgaaaa gctcgaaaaa tttatgaaag
     1081 gcaagcccgt tgccgagcag gaaataggaa agcaactttt actcgaaaca gtatcgtttg
     1141 ccgaatcatc tgtttgtcgc agaaaaatgc tgctacatta ttttggtgaa gaatacactc
     1201 aggaaaattg tggcaattgc gataattgtt tacatccaaa atcaaagata gaagcacagg
     1261 acgaagtcat aaccatgctc gaaacagttt tggctgtgaa agaaaaacat gtagcagagc
     1321 atgtgataaa tgtaataatg ggcgtgagcg aaaagagtgt gaaagactat ggtcacgaca
     1381 aactcgattg ctttgccgat ggtgccgatt atgatgcacg tttttggatg gctgttatca
     1441 gacaagcgtt ggtggcaggt tttatgtaca aagaagtaga aaattacggc ttactgaaac
     1501 ttaccgacaa gggcaagaaa tttattgcct cgccacattc attcatgatt accaaagacc
     1561 atgaatacaa caacaccgat gaagaggaag atgatgaaat gggcggtggt ggtgccggtg
     1621 gtggcggtgg tgccgatcca caaatgtttt cgatgctgaa agatttgcgc aagaaaattg
     1681 ccaagcaaaa aaatgtgcct ccatttgtgg tgtttcagga tccttcgctc gaagaaatgt
     1741 ctattcaata tcctatcacc attgatgaat taaaaaacat taccggtgta ggtgcaggca
     1801 aagcggtgaa atacggtaag ccatttattg agctcattgc aaaatatgtg gaagaaaatg
     1861 aaattgaacg cgctgtagat atggtggtga aaagtgtggt aaacaaatct ggattaaagg
     1921 tttatatcat acaaaatatc gaccgcaaaa ttgccctcga tgatatggcc gaatccaaag
     1981 gtattacaat gagcgattta attacagaga ttgaaagcat tgtaggtagc ggaacaaaaa
     2041 tagacatcaa ctattacctc aacgaagtaa tagatataga taaacaacaa gaggtattcg
     2101 actttttccg tgcagccgaa accgactcgg tacgcgatgc ccttaccgaa ctgggcgaag
     2161 atgaatacac cgaagaagaa atacgattga tgcgcattcg atttatgagt gagttgggta
     2221 attaattcac ttatggatct aataccaagc cccgaaatgt ttcggggctt tttttatgac
     2281 gacttttgtt tgttattgac tcgaagaaaa tgtgcgccta tccttgaatt aaattcctca
     2341 actttgttcg atatagtaat tgttataact gaaaataagg aacactattt attggctttg
     2401 attacacaat tgcaaaatca aatagcagag ttgagagaga aatttgaata gttgagaatg
     2461 tttagaaagt ttagaaagtg ctacggtgca aatgttgtgc agcgccaatt gcgaaatgga
     2521 atatgatgga gatacatttg taaaaagaat atagcattgc ttcttttgca cagcgacact
     2581 gtttcaacat ttaaaaaata atatttatcg caaacgcatt tgttttgcca accc
//
LOCUS       DAORQA010000243         2632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_8990_length_2632_cov_11.229724, whole genome shotgun sequence.
ACCESSION   DAORQA010000243 DAORQA010000000
VERSION     DAORQA010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8990_length_2632_cov_11.229724"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2347)
                     /locus_tag="PK736_08625"
     CDS             complement(<1..2347)
                     /locus_tag="PK736_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HRC33491.1"
                     /translation="MKKIILSLLLSCIGFTTMHAQKNMVFKSKKTYNGALSNIGGIAI
                     NGAEYALVGWEFGLSIVDVTDPNNIFEVVNIPGSQSIWREVKVWNNHAYVTTEAGGGL
                     QIINLNSLPSPNLPYKNWTGNNAIFGQIDNIHALHIEAGYVYLFGSNLFNGAALICDL
                     NDPWNPNYLGHSQGTYIHDGYVRNDTLFSCHIYDGYFSIINVANKANPVTINTQNTPD
                     NFTHNSWLNDAGNALFTTDEVDNSVLASYDISDPNNIILLDKFQTAPGSQAIVHNTHT
                     LNDYAVVSWYKEGVVVVDISHPDNMIEVGHYDTSPLSGGGFDGCWGVYPFLPSGNIVA
                     SDISEGLFVLAPTYVRGCYLEGTATDANTSAPLSSVQVEILSTSVTKFSKTTGVYKTG
                     YSNAGTYSVKYSKLGYISQTINNVVLQNGVVTIQDVQLVPISTVSISGSVIDGSTNLP
                     IPNAQVNLTDGAYNYDLVTDNAGNFNVSNFVTGTYTVTAGMWGYVTDCSLTTTINGGN
                     NVVVTLQPGWYDDFVFDFGWTKTSTASTGDWVREEPVGTFNNNGSLANPELDKSNDCN
                     DKCYMSGNGGGGSGSDDIDDGGVGLSSPIFDLTNIISPKIYYSRWIYNGGGNGTPNDT
                     LYFYLSNGTQEVLLEKVTISNINASKWTDVNFLISNYITPTATMKLRVYAEDFGQGNV
                     FEVALDKFFVTFGNVGVNENGTLANALSVFPNPSNSSFNITYNLSELSNAFLQIEDIT
                     GRVVEAKNIASIAGNIEIGSELENGAYTLRLINSDKIIRTVK"
BASE COUNT          828 a          552 c          445 g          807 t
ORIGIN      
        1 ttttaacagt acgaatgatt ttatcactgt tgattaaccg tagcgtataa gcaccatttt
       61 cgagttcact gccaatttca atgtttccgg caattgatgc aatatttttt gcttccacta
      121 ccctacctgt aatgtcttca atttgcaaaa atgcatttga caattcactc aaattataag
      181 tgatattgaa agatgaatta cttggatttg gaaacacaga taaagcattt gcaagggtac
      241 cattttcatt aactcccaca ttgccaaagg ttacaaaaaa cttatccaat gccacttcaa
      301 aaacattacc ctgtccaaaa tcttcggcat acacacgtaa cttcattgta gctgttggag
      361 taatataatt gcttatcaaa aaatttacat cggtccattt cgaagcattg atatttgaaa
      421 tagttacttt ttctaataac acttcctgtg ttccgttcga caaataaaaa tacaacgtgt
      481 cgttaggagt accattacca ccaccattat atatccatcg tgagtaatat atttttgggc
      541 tgataatatt tgtcaaatca aaaataggag atgaaagtcc aacaccgcca tcatcaatat
      601 catcggaacc ggaaccaccg ccaccatttc cactcatgta acatttatca ttacaatcgt
      661 ttgatttatc gagttcagga tttgcaagac taccgttatt gttgaatgta ccaacgggct
      721 cttcacgcac ccaatcgcca gtgctagctg tagaggtttt tgtccatcca aaatcaaaaa
      781 caaaatcatc gtaccaaccc ggttgcaaag taaccaccac attatttcca ccgttgatag
      841 ttgttgtaag tgagcaatcg gtaacataac cccacatacc ggcagtaacg gtataagtac
      901 cggtcacaaa atttgacaca ttaaaattgc ctgcattgtc tgtcactaaa tcataattgt
      961 atgcaccatc agtcaggtta acctgtgcgt tgggtatagg caaatttgta gaaccatcaa
     1021 tcacacttcc actgatagaa acggttgaaa taggcaccaa ttgaacatcc tgaatagtta
     1081 caacaccatt ttgcaaaacc acattattta tagtttgtga tatataacca agctttgaat
     1141 atttcacgct ataagtgccg gcattagaat aacctgtttt atatactccg gtagtcttcg
     1201 aaaattttgt aaccgaagta cttagaattt caacctgcac actgcttaat ggtgccgaag
     1261 tatttgcatc ggttgccgtt ccttcgaggt aacatcccct tacatatgtt ggcgcaagca
     1321 caaacaatcc ttcgctaata tcgctggcaa caatattgcc cgaaggcaaa aacggataaa
     1381 ctccccagca accatcaaaa cctccaccac tcaatggtga agtatcgtaa tgcccaacct
     1441 caatcatatt atcgggatgc gaaatatcaa ctaccaccac tccttctttg taccatgata
     1501 ctacagcata atcgttaagt gtatgtgtgt tgtgcacaat ggcttgcgag cccggtgcag
     1561 tttgaaattt atcaagcaaa ataatattgt tggggtccga aatatcgtag cttgccaata
     1621 cagaattatc aacttcgtcc gttgtaaaaa gtgcattgcc ggcatcgttc aaccaactgt
     1681 tgtgtgtaaa attatcagga gtgttttgtg tattgatggt tacaggattt gctttgttgg
     1741 caacattaat tatagaaaaa tatccgtcat aaatatggca cgaaaacaag gtatcattcc
     1801 gcacataacc atcgtggata tacgtacctt gtgagtgtcc taaataattg ggattccaag
     1861 ggtcattcaa atcgcaaatc aatgcagcac cattaaataa attactgcca aaaagataca
     1921 cataaccagc ttcgatatgc aaggcgtgaa tgttatctat ctgcccgaaa atagcattat
     1981 taccagtcca gtttttgtat ggtaagtttg gtgatggcaa agagttgagg ttaataattt
     2041 gcaagcctcc ccccgcttcg gtggtaacat aagcatgatt gttccatact tttacctcac
     2101 gccatatact ttgcgagccc ggtatattca ccacttcgaa aatattgttg gggtctgtta
     2161 catctactat tgataaacca aattcccagc ccaccagtgc atattcagca ccattaatgg
     2221 caatgccacc aatgttcgac aaggcaccat tgtaggtttt cttcgattta aacaccatgt
     2281 ttttttgcgc atgcatagtt gtaaaaccaa tacagctaag caatagcgat aagataattt
     2341 ttttcattaa gttttgtttt ttgttctaaa attttcagcc tgcgaagata aaatttattc
     2401 ctttagataa taggtattta gtagattatt ttgaatgacg tttgtggcaa aatatttcgg
     2461 cattctttgt ggcacttatg tccattttaa taatacaaaa catttcggct aaagccaaat
     2521 acaaattatt tattccgtcc gataaatcgg acggcaatga tgggctgacg cgaataacaa
     2581 ctttgtgcta aaaataatat ggtaagcttc gcccgaatac gttttcagtg ct
//
LOCUS       DAORQA010000244         2619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9047_length_2619_cov_7.906786, whole genome shotgun sequence.
ACCESSION   DAORQA010000244 DAORQA010000000
VERSION     DAORQA010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9047_length_2619_cov_7.906786"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..2321
                     /locus_tag="PK736_08630"
     CDS             114..2321
                     /locus_tag="PK736_08630"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HRC33492.1"
                     /translation="MGKLRSNWKSIYHIDLDNLVQPLLFRILSSYLDQGISIWDFPMH
                     EDGFLASLIALEKNSFTSFFKTNRARQLLFKANITINELLEIVVGDERYYEQYIFDQQ
                     FSHRGWSGMVAALEANPQSLLDRKKITLSDLLFFELLLEIDNLENQLGKNFQPLCSAI
                     NQAPINLFDNVENTELHEVLKIWQNAFEWSYYDEMLAGIEQNISLQSQKEITPAVKSF
                     QALFCIDERECSLRTHVESNDKNAVTFGCPGFFGVEFYFQAEHAKFYDKLCPAPVTPK
                     YLIKEYDVSEKRKHDLFYTNKTHQIASGYFSALIFGFWAGFRLIKNLFKPEMGPAISN
                     AFAHMNEQGKLTIENKNIADTENGLQIGFTILEMTNRVQALLNGIGLINDFAPIIYVV
                     AHGSSSANNPHHGAHDCGACSGRPGSVNSRVFASFANHTEVRKLLKERGIDIPLTTQF
                     VGALHDTAADEIEYYDVAILNEKNKIAHSQHQQTFESALDLNAKERSRRFASINTKAE
                     LKSIRKAIKERSVSLFEPRPELGHGTNTLCIVGNREMTRGVFLDRRSFLNSYNYKTDP
                     SGQYLTGVMAPLGPVCGGINLEYYFSRVDNYKLGAGTKLPHNVVGLFGVANSSDGDLR
                     PGLPWQMIEVHDPLRLLIIVEHYPELILKSIQSTPAMYEWYLNEWVHLISINPDTKAL
                     AYFKDGAFTPYKTLAKEIHFYKDVHSLIEEAREMKTNHIVHATQENLPVYLHN"
     gene            2329..>2619
                     /locus_tag="PK736_08635"
     CDS             2329..>2619
                     /locus_tag="PK736_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33493.1"
                     /translation="MEIILQLFIFLPFAGFIVSLFFNNTQEQLISRLSFICVGLQLIG
                     ITLFNIYWLWQQSPVINLKHFVFFKEDTIEIFLSFYFDKTTAVFSFLGALVTF"
BASE COUNT          843 a          441 c          516 g          819 t
ORIGIN      
        1 gtcgggtgtt gtgcgcgaaa aatcgtgtcg tataatttgc caaccatcgc cacctgcatc
       61 attggaaaaa cattgttcta tcaaaacaat atgatgaaca caatgatcag cgaattggca
      121 agctaagatc aaattggaaa tctatttatc acattgattt agataatttg gtgcaaccac
      181 ttttatttag aatactaagt agctatttgg atcaaggtat atccatttgg gatttcccta
      241 tgcatgaaga tggattttta gcttcgctta tcgccttaga aaaaaatagc tttacaagtt
      301 tcttcaaaac caacagggcc aggcaattat tgtttaaggc aaacattacc ataaacgaat
      361 tattggaaat agtggttggt gatgaaaggt attatgagca atatattttc gatcagcagt
      421 ttagccatag aggatggagt ggtatggtgg ctgcactgga agcgaatcct caaagtttgt
      481 tggatagaaa aaaaataaca cttagcgatt tacttttctt tgaattgttg cttgaaatag
      541 acaaccttga aaatcaatta gggaaaaatt ttcaacccct ttgcagcgcg ataaatcaag
      601 caccaataaa tttatttgac aatgtagaaa ataccgaact acatgaggta ttaaagatat
      661 ggcaaaatgc atttgagtgg agttattatg atgaaatgct tgccggaata gaacaaaata
      721 tttctcttca atcacaaaaa gaaattacgc ctgccgtcaa gtcgtttcaa gcattatttt
      781 gtattgatga gcgtgagtgc agtttgagaa cgcatgttga aagtaatgat aaaaacgcgg
      841 taacctttgg ttgcccgggc ttttttggtg tagaatttta tttccaggca gagcacgcaa
      901 agttttacga taagctttgc ccggctcccg ttacacccaa gtacttaatt aaggagtatg
      961 atgtttcaga aaaaaggaaa cacgatttat tttataccaa caaaacacat caaatagcat
     1021 ctggctattt tagtgcatta atttttggtt tttgggccgg atttcgttta attaaaaact
     1081 tattcaagcc cgaaatgggg ccggcaattt ccaatgcatt tgcgcatatg aatgagcaag
     1141 gtaaattgac aatagaaaat aaaaatattg ctgatacaga aaatggtttg caaataggtt
     1201 ttactatcct tgaaatgact aaccgggtgc aagcactgtt aaatggtatt ggattgatta
     1261 atgattttgc acctattatt tatgttgttg cacatggaag cagcagtgcc aataatccgc
     1321 atcatggtgc gcacgactgt ggcgcttgca gtggccgccc cggctctgtt aattcaagag
     1381 tatttgcatc ttttgccaat cataccgaag taagaaaact attgaaagag cgaggaattg
     1441 atattccgtt aaccactcaa tttgtaggcg cattgcatga tactgctgct gatgaaattg
     1501 aatattatga tgtagcaatc ttaaatgaaa aaaacaaaat tgcgcatagt caacatcagc
     1561 aaacatttga aagtgcactg gatttgaatg ccaaggaacg ttcgcgcaga tttgcgtcta
     1621 tcaatacaaa ggcagaatta aaatcaattc gcaaagccat taaagaaagg tcggtttcgt
     1681 tatttgaacc acgtcctgaa ttagggcacg gcaccaacac gctttgcata gttggcaata
     1741 gagaaatgac gcgtggcgtg tttttggata gacgctcttt tttaaattca tacaattata
     1801 aaaccgaccc aagtggtcaa tatttaactg gtgtaatggc gccattaggc ccagtttgtg
     1861 gtggtattaa tctcgagtat tattttagca gagttgataa ttataaatta ggagcgggaa
     1921 caaagttgcc gcataatgtt gtaggtttgt ttggtgttgc caacagcagt gatggtgatt
     1981 tgcggcctgg tttgccatgg caaatgatag aagtgcatga tccacttcgc ctattaataa
     2041 tagtagagca ttaccctgaa ctgatattaa aatctataca atctacacct gcaatgtacg
     2101 agtggtattt gaacgaatgg gtgcatttaa tttcaataaa cccagataca aaagcgctcg
     2161 catattttaa ggatggtgca tttactcctt acaaaacgtt ggcaaaggaa attcattttt
     2221 acaaagatgt gcactcgcta atagaggaag ccagggaaat gaaaacaaac catattgtac
     2281 acgccactca agaaaatttg cccgtttacc ttcacaatta atctaacaat ggaaatcata
     2341 cttcaattat ttattttttt accatttgct ggttttattg tcagtttgtt tttcaataat
     2401 acacaagaac aattaatttc ccgactatcg tttatatgtg taggccttca attaattggt
     2461 atcactttgt tcaacattta ttggctatgg cagcaatcac ctgttataaa tttaaaacac
     2521 tttgtgtttt ttaaagaaga tacgattgaa atatttctta gtttttattt tgataaaacc
     2581 accgcagtat tttcgtttct tggcgcactt gttactttt
//
LOCUS       DAORQA010000245         2591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9161_length_2591_cov_5.156940, whole genome shotgun sequence.
ACCESSION   DAORQA010000245 DAORQA010000000
VERSION     DAORQA010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9161_length_2591_cov_5.156940"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>825)
                     /locus_tag="PK736_08640"
     CDS             complement(<1..>825)
                     /locus_tag="PK736_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRC33494.1"
                     /translation="RQWLYIILPLFPLVMGYNIFARSFNIIIKDEVETNRPKINLLTY
                     NTRLFDLYNWSHNLETRAKMFNYIKDTKSDIICLQEFYTDDNPKSKFSNIDTLKQITG
                     LKYETHHYTTTLHKTEHWGIVTYSRFPIINSGVFDFDGPNNNSCIFSDFIVRGDTFRV
                     YNLHLESIRFAKNDYTFIDNIEKEKTENFNWRYAGTIAKKLMTASVARGKQAERIHQH
                     ILKCPYPILVCGDFNDTPASYTYSVISPYLKDAFHSGKIGFGATYNGPFPAFRIDQV"
     assembly_gap    826..837
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1056..1748)
                     /locus_tag="PK736_08645"
     CDS             complement(1056..1748)
                     /locus_tag="PK736_08645"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRC33495.1"
                     /translation="MFKPWSYFTYMFFHTDVFHILFNMLALYWIGNLFEEYLGSKKLV
                     AVYIVSGLCGGLLFQLAYNTLPLFSSVAAGSQLIGASAGIVGLMVATAVLIPNHEVML
                     ILLGQVKLKWLVIVLVLLFYFNIPKSNAGGEISHLGGALFGYLFMIYLRKGTDLTKGM
                     QNILGIFTNERKTKMRILEKTKLNDEQYNQRKKIMEETVDSILDKINKSGYNSLTKEE
                     KDILYRASNRKN"
     gene            complement(1751..1939)
                     /locus_tag="PK736_08650"
     CDS             complement(1751..1939)
                     /locus_tag="PK736_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33496.1"
                     /translation="MSKWLNELKITFSTNNATMKLLMINVAVFVVYNIIFVTELFFNS
                     NAPVSSLISNNLSLPSSF"
     assembly_gap    2144..2155
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2156..>2591)
                     /locus_tag="PK736_08655"
     CDS             complement(<2156..>2591)
                     /locus_tag="PK736_08655"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF020140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRC33497.1"
                     /translation="SIDLWHMMGNMFGLYFFGSHLERFWGTRRFVIFYFVCLLGAVII
                     YWGYNMYSQSAMVSETKMTIDNITPFDFYNYISKHEEVKESIGKENLDKFYSAWKQHP
                     NDTSFENEAKGYLNNILDSLTNGSRIVGASGAVFEILLAFAML"
BASE COUNT          867 a          468 c          433 g          799 t
ORIGIN      
        1 cacctgatcg attcggaagg ctgggaatgg accattgtaa gtggcaccaa agccaatttt
       61 accactgtgg aaagcatctt tcaagtatgg agaaataaca ctgtaagtat acgatgcggg
      121 agtatcgtta aaatcgccac aaactaatat cgggtaaggg catttcaata tatgttgatg
      181 tatacgttcg gcttgcttgc ctcgtgctac cgatgcagtc attaattttt tagctattgt
      241 gccggcatat ctccaattaa agttttcggt tttttctttt tcaatgttgt caataaaagt
      301 ataatcgttt tttgcaaacc gtatgctttc taaatgcaaa ttataaacac gaaatgtatc
      361 gccccgaaca ataaaatcag aaaaaataca actgttatta ttgggtccat caaaatcaaa
      421 cactcctgag ttgataatgg gaaaacgaga gtaagttact atgccccaat gttcggtttt
      481 gtgcaatgtg gttgtataat gatgtgtttc atatttcaat cctgtgattt gtttcaaagt
      541 atcaatattg gaaaatttcg acttcggatt atcatcggta taaaattcct ggaggcaaat
      601 aatatccgat tttgtatcct ttatatagtt aaacatttta gcacgcgttt ctaaattgtg
      661 cgaccagtta tacaaatcga aaaggcgtgt attgtaagta agcaaattta tcttcggccg
      721 attggtttcc acttcatctt ttatgataat attgaaactg cgtgcaaaaa tattgtaccc
      781 cattaccaat ggaaaaagag gaagtatgat gtaaagccat tgccgnnnnn nnnnnnncca
      841 accagtaaat ggcaagaata aaattgatga taaaaaaata ttgaaagaat aaagaaaaca
      901 aaatagcaat gggccatttt tcgggattga gtgatggcgc cattatagat aatgtaagca
      961 gcacagcaaa tagaattgac attattacaa gtgtaatatg gcaggctctt cgtattttaa
     1021 taatgatatt actttgcaat ggtttgggta ttggttcaat ttttacggtt actggcgcgg
     1081 tacaaaatat ctttctcttc ctttgtcaaa ctattgtatc ccgacttatt tattttatct
     1141 aaaatagaat caaccgtttc ttccattatt ttttttcgtt gattatattg ttcgtcattc
     1201 aattttgttt tttcaagaat gcgcattttt gttttgcgct catttgtaaa aatacctaaa
     1261 atattttgca tccctttagt gagatcggtt cctttgcgta aataaatcat aaatagataa
     1321 ccgaacaaag cgccacctaa atgcgatatt tcgccaccgg cattactctt cggaatattg
     1381 aaataaaaca atagtaccaa aacaataacc agccatttta attttacctg accaagtagt
     1441 atcaacatca cttcatggtt gggaataagc actgccgtgg caaccattaa ccctactatg
     1501 cctgccgatg cgcctattaa ttgcgaaccc gctgccacac ttgaaaacaa aggcaaggta
     1561 ttatatgcca actgaaataa caaccctccg cacaagccac taacaatgta tacagcaact
     1621 aattttttac tgccgagata ttcttcaaat aaatttccta tccaatatag tgcaagcata
     1681 ttgaatagaa tgtggaaaac atcggtatga aaaaacatat aagtaaagta gctccagggt
     1741 ttaaaaatta ctaaaaagaa gagggcaaac ttaaattgtt tgaaatcaat gatgataccg
     1801 gggcattgga attaaaaaat aattcggtca caaaaataat gttgtacaca acaaaaacag
     1861 ctacattaat catgagcaat ttcatggtag cattattggt agaaaaagta attttaagtt
     1921 cgttcaacca cttactcata agtctgttgc gattgatatt tttttagaat aattagttat
     1981 cgatgccata gttttaccag cacaaatgca gttagcatac cacccaaatg tgctatatgc
     2041 gcaacactgt cgcccggaac atttgcaatt acagaataga attcgacaat gccatataaa
     2101 agaacaaaat attttgcttt gataggaaaa ccaattaaag gagnnnnnnn nnnnncaaca
     2161 tggcaaatgc cagcaatatt tcaaatacgg cacccgaagc acctactatt cttgagccgt
     2221 tggtcaacga atcgagtatg ttgtttaaat atcctttagc ttcattttca aacgaagtat
     2281 catttggatg ttgtttccat gcagagtaaa acttatcaag gttttctttt ccaatagatt
     2341 ctttaacctc ttcgtgcttc gaaatataat tgtaaaaatc gaatggtgta atattgtcaa
     2401 ttgtcatttt ggtttcgcta accatggcac tttgcgaata catattgtat ccccagtaaa
     2461 taataaccgc tcccaacaag cacacaaaat aaaaaattac aaacctcctt gtgccccaaa
     2521 aacgttcgag gtggctgcca aaaaaataga ggccaaacat attacccatc atgtgccaca
     2581 aatctatgct t
//
LOCUS       DAORQA010000246         2581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9195_length_2581_cov_4.223674, whole genome shotgun sequence.
ACCESSION   DAORQA010000246 DAORQA010000000
VERSION     DAORQA010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9195_length_2581_cov_4.223674"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2290)
                     /locus_tag="PK736_08660"
     CDS             complement(<1..>2290)
                     /locus_tag="PK736_08660"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC33498.1"
                     /translation="SQMIQSINGKILRIDPNTGNGVPSNPYYDPNNPASAQSKVWCLG
                     LRNPYTISLKPGTGSTNPADGNPGTLYIGDVGWDTWEELNIADKPKLNFGWPIFEGLD
                     YCPGYGPLITPNYLATNPLFGMGGCTQQYFNFQELLKQATLDTTITFRNPCDTSQIIP
                     DSINTFFHARTALEFRHHYDSTQVGIFNGNNAAIISVDDSLSPCKGEPFGGDCIIDGM
                     FYQGDSFPSWYKGKYFFGEFEDEWIRAMTLDTNDKPTAVNLFAESTGYITHITENPFD
                     GTLVYVKFPDEIHKICYACMPDMPPVAIALADTTLGGDTLTVNFDGSKSSDPDNSLLS
                     YSWNFGDNTTSTLMNPQHFYTASAPGVSTTYIAVLTVTDDSSHTSSDSIYITLNNTAP
                     VVNISSFNDGDLFSSNNTSALPLSANVFDAEQDSTQLFYEWKVILHRNGNDSVVMIDN
                     NKISTVLLIPQVCPAVDVYYKIIFTVTDIYGETGIDSGSVYPDCNIPAAQISFANVGL
                     CQNDSVYFIDASLHFPIAWLWTFTGGSPAASTLQSPVVYYTQPGIYSVTLVVTNILGI
                     DSITQNYTVNTNPTVNLGNDTAVCANTYLMLNASNVGATYLWSTGNTIQTININVAGT
                     YTVTVVDTNGCKANDEIVISTYTLPVVSISTLNDTVCVQAGVVNIAGLPAGGIFNGSG
                     MTGNVFDPAAAGLGSHTAQYTYTDSLGCSNFDSVAIWVDACLAIPVTDNNGVKIYPNP
                     THDKIYISTGNISSGFNFVLSEM"
     assembly_gap    2291..2317
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2318..>2581)
                     /locus_tag="PK736_08665"
     CDS             complement(<2318..>2581)
                     /locus_tag="PK736_08665"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33499.1"
                     /translation="YNATNARVTRYTADPSSGFNKVVPGSRKVIIGSTKFNGIPVTHK
                     SHGSCAMVFGTDGTLLIATGDGAAYGAADNGAPGVTYSVQALL"
BASE COUNT          790 a          524 c          476 g          764 t
ORIGIN      
        1 catttcactc agcacaaaat taaatccgga gctaatatta cctgtgctga tataaatttt
       61 atcgtgagtt ggatttggat aaattttaac accattgtta tcagtaaccg gtatagccag
      121 gcaagcatca acccatatgg caactgagtc gaaattgctg catcctaaac tatcagtata
      181 agtgtattgc gcagtatgag aaccaagtcc tgcagcagca gggtcaaaaa cattacctgt
      241 cattccgctg ccattgaata ttccacctgc tggcaagcct gcaatattta caactcccgc
      301 ctgaacacaa actgtatcgt ttaatgttga tattgaaacc acaggcaaag tataagtact
      361 tattacaatt tcatcatttg ctttgcagcc atttgtatca acaacagtaa ccgtatacgt
      421 accggctaca tttatattaa ttgtttgaat ggtgttaccc gtactccata aataagttgc
      481 gcccacattg gatgcattca acatcaaata tgtattggca caaacagcag tatcgttgcc
      541 caggtttact gttgggttag tatttactgt ataattttgc gttatggaat cgatacccaa
      601 aatattggta acaactaatg tgacagaata tatacctggc tgggtgtagt aaactaccgg
      661 agattgtaac gttgatgcag cagggcttcc tcctgtaaaa gtccaaagcc atgcaattgg
      721 aaaatgcaac gatgcatcaa taaaataaac agaatcgttt tgacacaggc caacatttgc
      781 aaatgaaatt tgtgctgccg gaatattgca atcgggatat acactgccac tatcaattcc
      841 tgtttctcca tagatgtcgg taactgtaaa aattatttta taatacacat ctacagccgg
      901 gcacacttgt ggtattagca acactgttga aattttatta ttatcaatca tcacaaccga
      961 atcgtttcca ttgcgatgca aaattacttt ccactcataa aataattgtg ttgaatcttg
     1021 ttccgcatca aaaacatttg cacttaaagg caatgcgctt gtgttattac ttgaaaacaa
     1081 atcgccatcg ttaaagctcg aaatatttac aaccggtgca gtgttattaa gtgtgatata
     1141 aatagaatcg gatgatgtgt gactactatc atcggtaacg gtaagcacag caatataagt
     1201 tgtagaaact cccggtgccg aagcagtgta aaaatgttgc gggttcatta atgtacttgt
     1261 tgtattgtcg ccaaaattcc atgagtacga taaaagtgaa ttgtcgggat cggacgattt
     1321 gctgccatca aaatttactg ttagtgtatc gccacccaaa gtagtatcgg ccaaagctat
     1381 tgccacaggc ggcatatcgg gcatgcatgc ataacatatc ttatgtattt catcaggaaa
     1441 ctttacataa accaaggtac catcaaaagg attttcggta atatgtgtta tatatccggt
     1501 actttctgca aataaattaa cggctgtagg tttatcattt gtatctaatg tcatggcacg
     1561 aatccattca tcttcgaact caccaaaaaa atattttccc ttataccatg aggggaaact
     1621 atcgccctga taaaacattc catcaataat acaatcgcct ccaaaaggtt cacctttaca
     1681 aggcgataac gaatcatcaa cactaataat tgcagcgttg tttccattaa aaattcctac
     1741 ctgggtacta tcataatgat gccggaattc taacgcagta cgtgcatgaa aaaatgtatt
     1801 tattgagtca ggaattattt gcgaagtgtc gcagggattt cggaaagtga tggttgtgtc
     1861 taatgttgcc tgcttcaaca attcttgaaa attaaaatac tgttgcgtgc atccacccat
     1921 tccaaacaat ggatttgttg ccaaataatt tggtgtaatt aatgggccat aacctgggca
     1981 ataatcaagt ccttcgaaaa taggccaacc aaaattcaac ttaggtttat ctgcaatgtt
     2041 caactcttcc catgtatccc aacctacatc acctatgtat aatgtaccgg gatttccatc
     2101 ggctggattt gtacttccgg tgccgggttt caaactaatt gtgtatgggt ttcttaatcc
     2161 aaggcaccac acttttgatt gtgccgatgc aggattattt ggatcataat atggatttga
     2221 tggaactccg tttccggtat ttggatcgat acgaaggatt ttaccattta tactttgtat
     2281 catctgagag nnnnnnnnnn nnnnnnnnnn nnnnnnntcg agcaatgcct gaactgaata
     2341 agtgactcct ggcgcaccat tatcggcagc gccatatgca gcaccatcac cggtggcaat
     2401 caacaaagta ccatcggtgc caaacaccat tgcacatgaa ccatgcgatt tgtgtgtaac
     2461 cggaatacca ttaaacttgg ttgaacctat gataactttt ctgctacccg gaaccacttt
     2521 attaaatccg ctcgaaggat cggcagtata tctggtaaca cgcgcattag ttgcattata
     2581 g
//
LOCUS       DAORQA010000247         2565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9259_length_2565_cov_4.810359, whole genome shotgun sequence.
ACCESSION   DAORQA010000247 DAORQA010000000
VERSION     DAORQA010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9259_length_2565_cov_4.810359"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..368)
                     /gene="mraW"
                     /locus_tag="PK736_08670"
                     /pseudo
     CDS             complement(1..368)
                     /gene="mraW"
                     /locus_tag="PK736_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944693.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
     gene            complement(370..837)
                     /locus_tag="PK736_08675"
     CDS             complement(370..837)
                     /locus_tag="PK736_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33500.1"
                     /translation="MTSMVGEYLVKMDGKSRIQFPSAFRRQLTEADMEKFYFRKGDDG
                     CLELYPATNFEAICAQINQMPPHREKTKNLQRRFNKGAVEAFLDSTNRILIPKELLKY
                     AEITDECSLFARGLFIEVWDKDKFEKISAGGDEEEYLEAMDMIHSNLLETMKK"
     gene            complement(1228..2382)
                     /locus_tag="PK736_08680"
     CDS             complement(1228..2382)
                     /locus_tag="PK736_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HRC33501.1"
                     /translation="MDLSYILNHLGENRDEYFGAVAPPVIQSSNFYFKTVEQMRNAFV
                     NEQSTSIYTRGNNPTIAMTAQKIAAMEGAEDALLLASGSAAIAMAVLSQVKAGDHIVC
                     VNHPYSWTHKLLTQFLPRFNISATFIDGRDVDNFKKATHPNTTLYYLESPNTMYFELQ
                     DLPAVTSLAKQNNIKTIIDNSYCTMLVQQPIKLGVDIVVYSATKYLNGHSDVVAGVIC
                     SNSETIKKIFHNEFMTLGAIISPWNAWLLMRGLRTLPLRINHISNSVVKIINYLKQHP
                     AVEKIYWPFDESNSQLTLAKKQMSKPLGLFSILLKTKETKQIQAFCESLKYFLMAVSW
                     GGHESLIIPAIAFHPPHDYPLNFVRFYIGLEETDVLISDLKDALTCIQTD"
BASE COUNT          806 a          492 c          417 g          850 t
ORIGIN      
        1 attcattcgc atatcaagtg ctccttcgaa ccgaatgcta aaaccacgat tcgcagtgtc
       61 aaactgatga gacgaaacgc ccaggtctgc aagtattcca tcaacttgtg gtgctgcatg
      121 aaattgcaga aactgcttca gataactaaa atttgcatgt acaagaacga atctatcatc
      181 ttcaatttta ttggcgattg catctttgtc ctgatcgaat gcaattaact ttccattgcc
      241 aatatttttt attatctctt tgctatgtcg cccgcctcca aatgttacat ctcgtatata
      301 cgccctgctg ttatacttag tccttctata ctttcgtgca acagaacagg ttgatggtaa
      361 ttgctcattt tactttttca tcgtttcgag caaattgctg tgtatcatat ccatagcttc
      421 caaatactcc tcttcatcgc cacctgcaga aatcttctcg aacttatctt tatcccaaac
      481 ctcaataaac aatcctcttg caaacaatga acactcatca gtaatttcgg catacttgag
      541 taattctttt ggtatgagaa ttctgttagt tgaatcgagg aatgcttcta ctgcaccttt
      601 attaaatctc ctttgcagat tttttgtttt ctcacgatgc ggaggcattt gatttatttg
      661 tgcgcatata gcttcaaagt ttgtagccgg atacaattca aggcaaccat catctccttt
      721 acgaaaataa aatttctcca tatcggcttc ggttagctgt ctgcgaaaag cacttggaaa
      781 ctggatgcgc gatttgccat ccatttttac caaatattca ccaaccattg aagtcattta
      841 tgtgcagaat tattttttta tgctctgcaa agaaaaaaca aaaaatggga atccaatgca
      901 cttcattgca cttttgttga aaacttttgt gaaattagtt ttaccacatt gattttgcta
      961 tacaataaaa acagaaaaac aattctttaa aaacttaaaa ggcctataaa taggcgatta
     1021 tagaccactt ttcacaaact accatttata accatgataa aaacgactag tttgattgag
     1081 caataatttt gtgcaacaaa gtgcagcaaa atattatttt ttatcggatt tgggttaaat
     1141 tttatcattt ttttgttttg ggtaacaaaa tttaacgtca atttttggca aataaacacc
     1201 acggtttatt gtcaatattt atcgatttta atctgtttga atacatgtta atgcatcctt
     1261 caaatctgaa attaaaacat ctgtttcttc gaggcctata taaaagcgaa caaagtttaa
     1321 tggataatca tgtggtggat gaaatgcaat tgcaggaata attaacgatt cgtgaccacc
     1381 ccaggaaaca gccatcagaa aatatttaag cgattcgcaa aaagcctgaa tctgttttgt
     1441 ttcttttgtt ttcaataaaa tagaaaacaa gccaaggggt tttgacattt gttttttagc
     1501 caatgtcaat tgcgaattac tctcatcgaa aggccaataa attttttcta cagcagggtg
     1561 ttgctttaaa tagtttataa tttttaccac agaatttgaa atatgattta tcctcaaagg
     1621 cagagtgcgc aaacctcgca taagcaacca cgcattccat ggagaaatga tggcgcctaa
     1681 agtcataaac tcgttatgaa atattttttt tatagtttca ctattactgc aaataacacc
     1741 tgccaccaca tcgctgtgcc cgttcaaata ttttgttgca gagtacacca ctatatcgac
     1801 acctaacttt attggctgtt gtaccagcat ggtgcagtaa ctattatcaa taatggtttt
     1861 aatattattt tgcttcgcaa gagaagtcac agccggcaaa tcctgcaatt cgaaatacat
     1921 ggtgtttgga ctttcgagat agtacagcgt agtatttgga tgtgtggcct ttttaaaatt
     1981 gtctacatct ctaccatcaa taaatgttgc tgatatatta aaacgtggca aaaattgtgt
     2041 cagtaactta tgtgtccaac tataaggatg atttacacaa actatatgat caccggcctt
     2101 aacttgcgac agcactgcca tagcaattgc agccgaacca ctggccaaca ataaagcatc
     2161 ttctgcgcct tccattgctg caattttttg tgcagtcatt gcaatagtag gattattgcc
     2221 acgggtgtaa atacttgtgg attgttcatt gacaaatgcg ttgcgcattt gttcaactgt
     2281 tttaaaatag aaattactac tttgaattac cggtggggca acagcaccaa aatattcatc
     2341 acgattttcg ccaagatgat tcagtatgta acttaaatcc atattcgctt ttgattaata
     2401 tgtgtgggct tttctaaaat caatgtatca gaattaaaag gagtaactaa aacctgccat
     2461 tgcattaaat ggaaatacag gataattata gtagcgctga tacttggcaa agccaacatt
     2521 atttatgtca acaaaaaaac tcagcaattt tgtataacga tattc
//
LOCUS       DAORQA010000248         2552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9314_length_2552_cov_12.365639, whole genome shotgun sequence.
ACCESSION   DAORQA010000248 DAORQA010000000
VERSION     DAORQA010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9314_length_2552_cov_12.365639"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PK736_08685"
     CDS             <1..1167
                     /locus_tag="PK736_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRC33502.1"
                     /translation="AENTVAIAMGYGRTASGRASNDVGKNAYPFVRIANGSMAYYTAG
                     AITKTIDDDYTLVTTQTHHTMMGREIVKESDLKSWQKNAKAGNPDLLVNTPDGPQKPI
                     DIDLWATKDQPGHPKPNHHWQMGIDLNSCIGCGACVVACQAENNVAVVGKTEIGNARE
                     MHWIRIDRYYSSNMTKAKAEKEGTGVVQMYGDMEIPTDDNPQVLFQPVMCQHCNHAPC
                     ETVCPVAATTHSSEGLNMMAYNRCIGTRYCANNCPYKVRRFNWFNYTENEKFPYTMYD
                     ATSRMVLNPDVSVRSREVMEKCTMCVQRIQDGKLQSKKESRRPADGEIKTACMQTCPT
                     DAITFGDGNNNQSKVTGMFAEERSYWLLEELNVQPSVFYQTKIWNREETAGAAE"
     gene            1249..>2552
                     /gene="nrfD"
                     /locus_tag="PK736_08690"
     CDS             1249..>2552
                     /gene="nrfD"
                     /locus_tag="PK736_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRC33503.1"
                     /translation="MHAVESPLRPALITGDKTMHQITEDICRPIEGKANKYWWMAFTV
                     SCAALLWWIICIGYTFGTGIGVWGLNKTVGWAWDITNFVWWVGIGHAGTLISAILLLF
                     RQKWRLSINRSAEAMTIFAVICAASFILGHMGRPWLMYWPFPFANQFGSLWPNFNSPL
                     VWDVFAISTYFTVSLVFWYSGLLPDFAAVRDRAKTKAMKFWYTILSFGWTGKAKNWQR
                     FEEMSLVLAGLSTPLVLSVHSVVSMDFATSVIPGWHTTIFPPYFVAGAIFSGFAMVQT
                     LLIITRKVLNMEDYITIYHIDMMNKIIVLTGSIVGVAYITEFFIAWYSGVEFEQYCFL
                     NRATGPYWWAYWSMMSCNVISPQLFWSASLRRNITFTFFMSIIVNIGMWFERFVIIVT
                     SLHRDYVPSSWVYYRATWVELGIFIGSLGLFFVPFLLFTRFFP"
BASE COUNT          750 a          488 c          561 g          753 t
ORIGIN      
        1 gccgaaaata ctgttgctat tgcaatggga tacggacgaa ctgcatcggg acgtgcttct
       61 aacgatgttg gaaaaaatgc ttatccattt gttagaattg ctaacggctc aatggcatat
      121 tatactgcag gagcaattac aaaaacaatt gatgatgatt atactttagt tacaacacaa
      181 acgcaccaca caatgatggg tcgtgaaatt gtaaaagagt cggatttgaa atcatggcaa
      241 aaaaatgcta aagctgggaa tcctgattta ttggtaaata caccagatgg ccctcaaaag
      301 ccaattgata tagatttatg ggcaacaaaa gatcaaccgg gtcacccgaa accaaatcat
      361 cattggcaaa tgggtatcga ccttaactca tgcataggtt gtggcgcatg tgttgttgct
      421 tgccaggcag aaaataatgt tgcagtagta gggaaaacag aaattggtaa tgcacgcgaa
      481 atgcactgga ttcgaataga ccgctactat tcatcgaaca tgacaaaggc caaagctgaa
      541 aaggagggaa caggtgtagt gcagatgtat ggcgatatgg aaattccaac tgatgataat
      601 cctcaagtat tgtttcagcc ggtaatgtgc cagcattgca atcatgcgcc ttgcgaaaca
      661 gtttgtccag ttgcagcaac tacacatagc agtgagggtt taaacatgat ggcatacaac
      721 cgttgtatag gtactcgtta ttgtgctaat aactgtccat acaaagttcg tagattcaat
      781 tggttcaatt ataccgaaaa cgaaaagttc ccttatacaa tgtatgacgc tacatcacgc
      841 atggtgctta accccgatgt atcggtgcgt tcgcgcgaag taatggaaaa atgtactatg
      901 tgtgttcaac ggattcagga cggcaagctg caatcgaaaa aagaaagtcg cagacctgca
      961 gacggtgaaa ttaaaaccgc atgtatgcaa acatgcccaa ccgatgcaat cacctttggc
     1021 gatggcaaca ataaccaaag taaagtaaca ggaatgtttg ccgaagagcg tagctattgg
     1081 ttactcgaag aattgaacgt gcagccatct gtattttatc aaacaaaaat ttggaatcgt
     1141 gaagaaaccg caggcgcagc cgagtaattt ttttacaaaa tcaaattgaa tcaataaaag
     1201 gaaataaaga catcagataa gtaataacga caaagaagta gaaaatatat gcatgcagta
     1261 gaatctccct taaggcccgc gttgattaca ggtgacaaga caatgcacca aatcaccgag
     1321 gatatttgcc gccctataga aggcaaggcg aataaatatt ggtggatggc cttcaccgtt
     1381 tcttgcgcag ctttgctatg gtggataatt tgtataggat acacatttgg cactggaata
     1441 ggcgtgtggg gcttaaataa aactgtcggt tgggcatggg atatcaccaa ctttgtatgg
     1501 tgggtaggta ttggacatgc cggaaccttg atttcggcca tcttattatt attccgtcaa
     1561 aaatggaggt tgagcatcaa ccgtagtgct gaggccatga ccatttttgc tgtaatatgc
     1621 gcagcatcat ttattcttgg ccatatgggt cgtccttggt tgatgtattg gccgttccct
     1681 tttgcaaatc aatttggttc gctgtggcct aactttaaca gcccgcttgt ttgggacgta
     1741 tttgccatat ctacttactt tactgtttcg ttggtatttt ggtatagtgg cttattaccc
     1801 gattttgccg ctgttcgcga ccgcgcaaaa accaaggcca tgaaattctg gtataccatt
     1861 ctttcatttg gttggactgg aaaagctaaa aactggcagc gtttcgaaga aatgtcattg
     1921 gtgcttgccg ggctttcaac tcctcttgta ttgtcggttc actcagtagt atccatggac
     1981 tttgccacat ctgttattcc gggatggcat acaaccatat tccctcctta tttcgttgcc
     2041 ggtgccattt tctctggatt tgccatggtg caaacgcttt tgataattac ccgcaaagta
     2101 ttaaacatgg aagattacat tacaatttac cacatcgata tgatgaataa aatcattgtg
     2161 ttaacgggtt caattgtagg tgtagcatat attacagaat tttttatcgc atggtattca
     2221 ggcgtagagt ttgaacaata ttgctttctc aatcgtgcta ccgggcctta ttggtgggct
     2281 tattggagta tgatgagttg caatgttatt tcccctcaat tattttggtc tgcttcatta
     2341 cgcagaaaca tcactttcac attctttatg tcaatcatag taaacatagg tatgtggttc
     2401 gagcgatttg taattatcgt aacatcactt caccgcgatt atgttccatc tagctgggtt
     2461 tattatcgtg ccacctgggt tgaattagga atatttatcg gctcactggg gttatttttt
     2521 gtaccatttt tattattcac aagatttttt cc
//
LOCUS       DAORQA010000249         2546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9341_length_2546_cov_7.759936, whole genome shotgun sequence.
ACCESSION   DAORQA010000249 DAORQA010000000
VERSION     DAORQA010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9341_length_2546_cov_7.759936"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PK736_08695"
     CDS             complement(<1..1087)
                     /locus_tag="PK736_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC33504.1"
                     /translation="MNFNTDENQQMIVQTVKDFAEKNIRPHVMEWDETQEFPIHIFKK
                     MGELGLMGILVPTEYGGAGLGYNEYVAAIVETARVCGSIGLSLAAHNSLCTGHIMQFG
                     NEEQKKKWLPKLASAEWIGAWGLTEPNTGSDAGNMMCVAKEDGDHYVINGAKCWITHG
                     KSSNIAVVIVRTGEKGDSRGMTAFAIEKGTPGFSAGRKENKLGMRASETTELIFDNCR
                     VHKDNMLGKVGEGFIQSMKILDGGRISIAALGLGIAKGAFDASVKYSKERQQFGKPIS
                     EFQGISFKLADMATEIEAAELLIMQACDLKNRGEKVTKLSAMAKYYSSEVAVRCATEA
                     VQIFGGYGYTKDFPVEKFYRDAKLCTIG"
     gene            1444..2445
                     /locus_tag="PK736_08700"
     CDS             1444..2445
                     /locus_tag="PK736_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HRC33505.1"
                     /translation="MQNSIGSVQQFKTSLAKLRVLVVGDVMMDAYTWGRVERISPEAP
                     VPVVNVLHREDRPGGAANVAINLNAMGVRTFLCGIVGNDANGKKLTSLIKKQKINTQG
                     MVSLSTHVTTVKTRIIGNNHQLLRIDDERHNELSNVQNEQFIKAIISTAKEHKVDAII
                     FEDYDKGALNKTVIEGVIEFALANNITTAVDPKKKNFFSYKNVTLFKPNLHELENGLQ
                     QAHISTNEIAALAKAFAKKQRIQSMLVTLSDKGIFYGDAKTSGIIPAHIRNIADVSGA
                     GDTVISVATCCLALQMPASQIAAIANIAGGLVCEKSGVVPILLNDLLQEIKHHSILA"
BASE COUNT          751 a          542 c          483 g          770 t
ORIGIN      
        1 cgccaatggt acaaagtttt gcatcgcgat aaaatttctc caccggaaaa tctttggtgt
       61 atccataacc gccaaatatt tgtaccgctt ctgtagcaca acgtaccgcc acttccgatg
      121 aataatattt tgccatagca ctaagtttag taactttttc gccacggttt ttcaaatcac
      181 aagcctgcat aatcaatagt tctgcagctt ctatctcggt ggccatatcg gcaagtttaa
      241 acgatatgcc ttgaaattcg gaaatgggtt tgccaaattg ctggcgctct ttagagtact
      301 ttactgatgc atcgaatgct cctttggcaa ttcctaaacc caatgccgca atggatatac
      361 gaccgccatc gagaattttc atcgactgta tgaaaccttc tcctacttta ccaagcatat
      421 tatctttatg aacacggcaa ttatcaaaaa tcaattcggt ggtttccgat gcacgcatac
      481 caagtttgtt ttctttgcgg cctgcgctaa agcccggtgt gcctttctct atggcaaatg
      541 ctgtcatacc acgcgagtcg cctttttcac ctgtacgcac tattactaca gcaatgtttg
      601 acgatttgcc atgcgttatc cagcacttgg caccatttat tacataatgg tcgccatctt
      661 cttttgctac gcacatcata ttaccggcat cgctccctgt gtttggttcg gtaagtcccc
      721 aggcaccaat ccattcggca ctggcaagtt ttggcaacca ttttttcttt tgctcttcgt
      781 taccaaattg catgatatgg cctgtacaaa gtgagttatg tgctgccagg ctcaaaccta
      841 tgctgccaca cactcttgcg gtttctacta tcgctgctac atattcatta taaccaagtc
      901 cggcaccgcc atattcggta ggaacaagta ttcccatcaa accaagttcg cccatttttt
      961 tgaaaatatg aatcgggaat tcttgagttt cgtcccattc catcacatga ggtcgaatat
     1021 ttttttcggc aaaatctttt accgtttgca ctatcatttg ctggttttcg tctgtattga
     1081 aattcataag tcttatttat aatcttgaaa atttgcgtgc gaaaataact tttttgacat
     1141 cattcttggc aatttttatt taagccataa aacatttttt tcgtttacat tactaagtgt
     1201 ccaattcaat ttgcgaaaag ccttattgat tgtaccttaa attctttctg agatgcttcg
     1261 ttcttcacta attcaaatcg aaaggctgct ctgcttaata acaataaacc agcaacccaa
     1321 taacaaataa cccaataaca aaataaccaa taaccaaata acccaatcat cccaccaaca
     1381 taattttcat atcaattgta aacaagtact tttgcgccac aatttttttg aaagaaataa
     1441 tacgtgcaaa attccatcgg ttcggtacaa caatttaaaa catcacttgc gaaattacgg
     1501 gtattggtag tgggcgatgt tatgatggat gcttatacat gggggagggt agaacgcata
     1561 tcgcccgaag cccctgttcc ggttgtaaat gttttgcatc gcgaagacag gccgggaggt
     1621 gcagccaatg tggccatcaa tttaaatgca atgggagtgc gcactttttt atgcggcatt
     1681 gttggtaacg atgctaacgg aaaaaagcta acatctctta tcaaaaagca aaaaataaat
     1741 acgcagggaa tggtatcgct tagcactcat gtaactaccg tgaaaacccg gattatagga
     1801 aacaaccatc agttgctgcg tatagatgat gagcgccaca atgaactttc taacgtgcaa
     1861 aatgaacagt ttataaaagc tataatcagc actgcaaagg agcataaagt agatgccatc
     1921 atatttgaag attatgataa gggcgcattg aataaaactg ttattgaagg cgtaattgag
     1981 tttgccctgg caaataatat tactactgcg gtagatccta aaaagaagaa ttttttcagc
     2041 tataaaaatg taaccctttt taagccgaac ctgcacgaac ttgaaaatgg tttgcagcaa
     2101 gcacatatat caacaaatga aatagcagca ttggcaaaag cgtttgccaa aaagcaacgt
     2161 atacagtcta tgttagttac actgagcgac aaagggattt tttatggcga tgcaaaaaca
     2221 tcgggcataa ttcctgccca tattcgcaat attgccgatg tgtcgggtgc cggtgatact
     2281 gtaataagtg tggctacatg ttgccttgcg ttgcaaatgc cggcaagcca gatagcagcc
     2341 atcgccaata ttgccggagg attggtttgc gaaaaatccg gtgttgttcc catattgttg
     2401 aacgaccttt tgcaggaaat aaaacatcat tcaattttag cataacatgt cgatagaaag
     2461 ttttcgcgaa tgggttaatt tgcgcatatt caaaagcaag tcgatggtga tgaatgtttt
     2521 tgatgtgctg agcattgtgg ttgcat
//
LOCUS       DAORQA010000250         2545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9349_length_2545_cov_5.856225, whole genome shotgun sequence.
ACCESSION   DAORQA010000250 DAORQA010000000
VERSION     DAORQA010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9349_length_2545_cov_5.856225"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2516
                     /locus_tag="PK736_08705"
     CDS             <1..2516
                     /locus_tag="PK736_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRC33506.1"
                     /translation="IEGPIKKDTSSFMISGRRTFIDLFLREPFIKKDAPAAGNSYFFY
                     DLNAKVNYRFSDKDRLFLSGYFGRDVFSFNSPSGGFKADIPWGNATGTLRWNHLFSDK
                     LFVNTSAIFTDYNFSFGAGQDDFEFKLFSGVRDWSLKSDFDYFPSILHHVKFGANYVY
                     HIFTPSSASAKQGDVEFDLGEPIKLRAHDFSAYITDDWSIDDKWTVTGGVRLTNFMFV
                     GPFDRYIVNEFFVVTDTIHYGKNTIVKNYPNAEPRFLAKYSLNSRSSLKASFTQNYQY
                     IHLASVSSVSLPTDVWVPSSDKVKPQKSTQYALGYFRNFLDDMFESSVEVYYKDMRNQ
                     IEYRDGASQDEDVKNNQDNNFVFGKGWSYGAEFFLKKVYGDFNGWIGYTLAYTNRQFD
                     EKNLGKAFPAKYDRRHDVSIVLIYSPNKKWTFGATWVYATGNATTLPQDRYLFASRIN
                     NIPNSLSYIVLSQTYISEGSQMIDVLDTYGETMSISIDELMQGSNLLSTYNYTGTNDP
                     FYEPYTFYTTLCNNENTYYVYDHLGNTRITYTPTVMPDGLKFTLNGVYDYYPYGKILH
                     EYVQGPQEKYLTTHHQRDEETGLDYRGARFYDCDVMRFLSSDPLARVAPAWNTYRAYY
                     CNPLIFIDPTGLLESTEVTDNGDGTYNVVNGNTKDNDKGIYVVDKNGVRTGEKIGESL
                     FIESFHAPGKDGGDGKWLGKIDTRSTEGVDFLNDEIIDPMMIITDYLAQNKKMDFKSR
                     GDQGTKSEQNANHYRGSEYSKGVYASARDFGNIGAGIVAGMSDYSWPAAKAAFEFREG
                     VKQSQYFQRVPEPACTVEAEKFGYDIGSKIKRPQQHRDW"
BASE COUNT          804 a          445 c          547 g          749 t
ORIGIN      
        1 ccattgaagg gccaataaaa aaagacacat cgtcatttat gatttcgggg cgcagaacat
       61 ttatcgattt atttttgcgc gagcctttta taaaaaagga tgcaccggct gccggcaatt
      121 cgtatttttt ttatgattta aatgcaaaag ttaattaccg tttttcggac aaagaccgtt
      181 tgtttttgag cggatatttt ggccgcgatg tattttcttt caatagtcca tcaggaggat
      241 ttaaagccga tattccctgg ggcaatgcta ctggaacttt gcgatggaat catttattca
      301 gcgataaatt atttgtaaat acttcagcta tttttacgga ctataatttt tcgtttggtg
      361 ccgggcaaga tgatttcgaa ttcaaattat tctccggtgt gcgcgactgg agcttaaaat
      421 ccgacttcga ttattttcct tctattcttc atcatgtaaa atttggtgct aattatgtgt
      481 atcatatttt tacaccaagt agtgcatcgg ctaagcaggg tgacgtagag ttcgatttgg
      541 gcgaacctat aaaattgcgg gcgcatgatt ttagcgcata cattacagac gactggagta
      601 tagacgataa atggactgta accggtggtg tgcgcctcac caattttatg tttgtgggcc
      661 cattcgacag atacattgta aatgaatttt ttgttgtaac agatactata cattacggca
      721 agaatacaat tgtaaaaaac taccctaatg ccgagccacg tttcttagca aaatactcgc
      781 tcaattcgcg ctcgagttta aaagcatcgt tcacacaaaa ttatcaatac attcatcttg
      841 catcggtgtc gagtgtttct ttacctaccg atgtgtgggt gccaagtagt gataaggtga
      901 aaccgcagaa aagcacgcaa tatgcactgg gttatttccg caattttttg gacgatatgt
      961 ttgagtcatc ggtagaggtt tactacaaag atatgcgaaa tcaaattgag tatcgcgatg
     1021 gtgcttcaca agatgaggat gtaaaaaata atcaggataa taattttgtt tttggtaaag
     1081 ggtggagtta cggtgcggag ttttttttga aaaaagttta tggcgatttc aatggatgga
     1141 ttggatatac gttagcatac accaacaggc agtttgacga aaaaaatctg ggaaaagcgt
     1201 ttcctgcaaa atatgacagg cgacatgatg tgagtatcgt attgatttac tctccaaata
     1261 agaaatggac atttggcgct acatgggtat acgccactgg caatgcaact acattgcctc
     1321 aggatcgtta tttatttgcc tcacgtataa acaacatacc aaacagctta tcatacatag
     1381 tgctatcaca aacatacata agcgaaggct cacaaatgat agatgtactc gatacctatg
     1441 gcgaaacaat gagcatatct attgacgaat tgatgcaagg cagcaacctt ttatctacat
     1501 acaactacac aggcaccaac gacccatttt atgagccgta tactttttac accacacttt
     1561 gtaataacga aaatacctac tacgtgtacg accatttagg aaatacacgc ataacgtata
     1621 cacccaccgt tatgcccgat ggtttaaaat ttactcttaa tggtgtttac gattattacc
     1681 cttacggcaa aatactgcac gagtatgtgc aaggcccgca agaaaagtac ctcaccaccc
     1741 accaccaacg tgacgaagaa accggcctcg attaccgcgg tgcacgtttt tatgattgcg
     1801 atgttatgag gtttttgagt tcggatccat tggcacgggt agcacctgct tggaatacgt
     1861 atagagcgta ttattgtaac cctttaatat ttattgaccc gaccggcctt ttagaaagta
     1921 cagaagtaac agacaatggt gatggcactt ataatgttgt taatggcaac acaaaagata
     1981 atgacaaagg catttatgta gttgataaaa atggggtaag aactggagaa aaaataggag
     2041 aaagtttatt tatagaatca tttcatgctc ctggaaagga tggaggtgat ggaaaatggc
     2101 taggtaaaat agatactaga tcaacagaag gagttgattt tttgaatgat gaaattattg
     2161 accccatgat gattataacg gattatttgg cgcaaaataa gaaaatggat tttaaatcac
     2221 gaggcgatca aggaactaag agtgaacaaa atgcaaacca ttatagagga agtgaataca
     2281 gtaaaggtgt ttatgcaagt gcccgagatt ttggcaatat tggagcagga attgttgcag
     2341 gaatgagtga ctattcttgg cctgctgcaa aagcggcctt tgaatttcgt gaaggtgtta
     2401 aacaatcaca gtatttccaa agagttcctg agcctgcatg tactgtagag gcggaaaagt
     2461 ttggttatga tattggaagc aagattaaac gccctcaaca acatcgagat tggtagtgat
     2521 cttttaagat gaaattgttg gaaat
//
LOCUS       DAORQA010000251         2542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9365_length_2542_cov_8.190189, whole genome shotgun sequence.
ACCESSION   DAORQA010000251 DAORQA010000000
VERSION     DAORQA010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9365_length_2542_cov_8.190189"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..>2542
                     /gene="sprA"
                     /locus_tag="PK736_08710"
     CDS             25..>2542
                     /gene="sprA"
                     /locus_tag="PK736_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRC33507.1"
                     /translation="MYFVKSFNDPSNITTTIDYDPTTNEYNINERVGNQFYRNPNYMT
                     FQEYVDDQSKKSTQAYWKQLAEGANIVSQKKGFNPKLYVPGKLFETIFGGNSIDIRPQ
                     GSVELRFGLQSNRNDNPALPEKQRKVTTFDFNQKIQLNVVGNIGTKLKTAINYNTEAS
                     FEFENRMKLEFTGGEDDILKKLEAGNVTLPLNGTLIQGSQSLFGIKAQLQFGHLTVTS
                     IFSQQKGKTSTIEVKGGAQTQQYELTCDNYEANRHFFLAQYFRDNYDAAMRNIPVIQS
                     PVTITKIEVWIVKPGQIDTRALIGLADAGESNPSNTNFLTPGVSPLPGNDANSLYSTL
                     NAPPYNQLRNVGFANFNILNQLNMVAPKDYERIDNAVKLNPNEYTFNPRLGFISLRSS
                     LNPNQVLAVAYEYQVNGKLYRVGDLTNSGVNPQDRMYVKMLKSSNLNVTLPIWDLMMK
                     NVYNIGAYGVQPKDFKLNILFFDDSTGTNINFLPVSATQPKLFGKPLLRVLNLDRLNS
                     QNDPQPDGVFDYVEGLTINSSDGRVYIPVVEPFGKSLQALFTDSASASKYMYQELYDQ
                     TKVAAQQITEKNKYRLKGTYSSSVSSDIALNQINIAQGSVKVSSGGIPLTENVDFTVD
                     YNLGRVKIINEAYLNSATPLQIRVESNSLFALQTRRLMGTHLDYQFSKDFQIGATIMN
                     LSERPITQKVNFGDEPISNTIWGLDGNYRTDSRFITKLIDKLPFISTKDISTVTISGE
                     FAHFIPGHPKVIGKGGVSYIDDFEGSQSSLDIRNAGNWYLASTPDKQPNLFPESLVLT
                     DSLASGKNRAKLAWYTIDRSVFYNGNNLKPAAVTDVELSKH"
BASE COUNT          825 a          541 c          496 g          680 t
ORIGIN      
        1 tcatgattgc tgatgtattc ataattgtat tttgtaaaat cattcaatga cccatcgaac
       61 ataacaacca ctatagacta tgaccccacc accaacgaat acaatataaa cgaacgagtt
      121 ggcaatcagt tttaccgtaa cccaaattat atgacatttc aggagtatgt ggacgatcaa
      181 agtaaaaaat cgacacaagc ctactggaaa cagcttgccg aaggggccaa tatcgtatca
      241 caaaaaaaag gctttaatcc taagttatat gtgccgggca agttattcga aaccattttt
      301 ggcggtaact caatcgatat tcgtccacaa ggttcggtag aattgcggtt tggcttgcaa
      361 agtaaccgta acgataatcc ggcattgccc gagaaacaac gcaaagtaac taccttcgat
      421 tttaatcaaa aaatacaatt gaacgttgta ggtaacattg gtacaaaatt aaaaacggct
      481 ataaattaca ataccgaagc gagtttcgaa tttgagaata gaatgaaact tgaatttacc
      541 ggtggtgaag acgacattct taaaaaactt gaagccggta atgttacttt acctctcaac
      601 ggaaccttga tacaaggcag ccaaagcctt tttggaataa aagcacaatt acaatttggc
      661 catcttacgg ttacatccat tttttcgcaa cagaaaggaa aaaccagcac tatagaagta
      721 aaaggcggag cccaaactca gcaatatgaa cttacttgtg ataactacga ggccaatcgc
      781 cacttttttc ttgcacaata ttttcgcgac aattacgatg ctgccatgcg taatattcct
      841 gtaattcaat cgcctgtaac tattacaaaa attgaagtat ggatagtgaa acctgggcag
      901 attgataccc gcgcattgat aggtttagcc gatgcgggtg agagcaatcc cagcaatact
      961 aattttttaa cccctggcgt ttcaccactt ccgggcaacg atgccaattc actttattca
     1021 acgcttaacg caccgccata caaccaattg cgcaatgttg gctttgcaaa tttcaatatt
     1081 ctcaaccagt tgaatatggt tgctcccaaa gactacgaac ggatagataa cgcagtaaag
     1141 ctaaatccaa acgaatatac attcaacccc cggttaggat tcatttcgtt acgttcgagc
     1201 ctcaacccca atcaagtatt ggcggtggct tacgagtatc aggtaaacgg aaaattatac
     1261 cgtgtgggcg atttaacaaa ctcaggagta aatccgcaag accgcatgta tgtaaaaatg
     1321 cttaaaagtt ccaatttgaa cgtgacattg cccatatggg acttgatgat gaaaaacgtt
     1381 tacaacattg gcgcttatgg ggtacagcca aaagatttta aattgaacat tttatttttt
     1441 gatgattcta cgggtaccaa catcaatttt cttccggttt cagcaacaca acctaaatta
     1501 tttggcaagc cattattaag ggttttgaat ctggacaggc taaactctca aaacgacccg
     1561 cagcccgatg gtgtgtttga ttatgttgaa ggcctcacca ttaactcctc tgacggtcgt
     1621 gtgtatatac ctgtagttga accatttggc aaatcgttac aggctttgtt taccgatagt
     1681 gccagtgctt caaagtatat gtaccaggaa ttatacgacc aaacaaaagt agccgcgcaa
     1741 caaatcaccg aaaaaaataa ataccggctc aaaggcacct actccagttc ggtaagtagc
     1801 gatatagccc tcaatcaaat aaatattgct cagggttcgg taaaagtaag ttcgggcgga
     1861 atacccctta ccgagaatgt tgactttacg gttgattata atttagggcg ggtaaaaata
     1921 attaacgaag cgtacctaaa ctctgctacg ccattgcaga ttagggtaga aagtaattcg
     1981 ctatttgcat tgcaaacaag gcgcttgatg ggcacacatc tcgattatca gtttagcaaa
     2041 gattttcaga ttggcgctac catcatgaat cttagcgaaa ggccaatcac acaaaaagtg
     2101 aactttggcg atgaaccaat ttctaacacc atttggggat tggacggcaa ctatcgcacc
     2161 gactcgcgct ttattaccaa actcatcgat aagctgccat ttatcagcac gaaggatatt
     2221 tctacagtaa ccatcagtgg agaatttgca cattttattc ccggccatcc aaaggtgatt
     2281 ggtaagggag gcgtatcata tattgacgat tttgaaggta gccaaagcag tcttgatatt
     2341 cgcaatgccg gcaattggta tttggccagt acacccgaca agcaacccaa tttatttccc
     2401 gaatcgcttg tgttgaccga cagccttgcc tcaggaaaaa acagagctaa gcttgcatgg
     2461 tacaccatag accgttcggt gttttataat ggtaacaatt taaaacccgc agccgttacc
     2521 gatgttgaat tatcaaaaca tg
//
LOCUS       DAORQA010000252         2530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9399_length_2530_cov_14.281616, whole genome shotgun sequence.
ACCESSION   DAORQA010000252 DAORQA010000000
VERSION     DAORQA010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9399_length_2530_cov_14.281616"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..2407)
                     /locus_tag="PK736_08715"
     CDS             complement(71..2407)
                     /locus_tag="PK736_08715"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRC33508.1"
                     /translation="MLKKTLQIIVFAICIHQASMSQGLPKEMYLSPDLRTLYTGGKPD
                     NGLYDQTQIKQVYLNFPQANYWTLLIQNYANKIELAAGMTVDGVLYDSVGVRFKGQTS
                     YSQTQNSQKKSFNITTDNFKPGQDIDGYNILNLNNCFDDPSFTREYFYLNQIKKHIPV
                     AKCAYVHLYINGQNWGLYPNVQQLNKDFYTEWFMSNNGTNWRADKPAGSPGNPGWGDG
                     TAALNFLGNDTSLYKQYYQLKSTNSATPWNDLRNGCSRLNKPSLANLPTMLPDSMDVD
                     RALWHLASETAFSDDDSYIFKGKMDYYVYYEPETHRITPIEYDGNSVMRISALNWSPF
                     YNETKVNYPLMNRLYAVPEWRQRYLAHLRTIISESFDTAVCNLFLNQNKAFIDSVVLN
                     DPKKLYSYTAFNTENTALKTWMNNRKNYLMSNIEVNQTGPAISNVSFATNSVTWTYPT
                     SADSVGVNATVTSGNGINKVTLFYATGIVGNFTSSLMYDDGMHGDASAADGIYGAYIP
                     AQNNSQWVRYYIEAKAGNSYKTAMYSPVGAEHDVYVYFVTPVLSSDTTITINEVMASN
                     SNTAMDNAGEYDDWIELYNKSGNPVDISGYFITDDTLNLEKFELPQGTIINPNNYLIL
                     WADEDGSQGPLHLNFKLSSAGERVWLLNPNRELVDKVEFGQQITDQGYARVPNGTGNF
                     VIQNPTFGYNNNLFSSVNENIIAPSIVNVFPNPANEFINIVTNQVSDLPVTIVNAIGQ
                     IKISLQGQPNYFVDTQSWQNGIYFIRYGENVVKIVVSH"
BASE COUNT          734 a          506 c          468 g          822 t
ORIGIN      
        1 ttgaatgacc ctaaaaaacc ctcataaagc cttgccatat tagggtttga agtaacactt
       61 aaaaatttta tcaatggctt actacaatct tcactacatt ttcgccatag cgaataaaat
      121 aaattccatt ctgccatgat tgggtatcaa caaaataatt tggttggccc tgcaacgaaa
      181 tttttatttg ccctattgca ttcacaattg taaccggtaa atcgcttacc tgattggtca
      241 caatattgat aaactcatta gcaggatttg gaaaaacatt tacaatactc ggagctatta
      301 tgttttcgtt taccgatgag aataaattat tgttgtaacc aaaagtagga ttttgaatta
      361 caaaatttcc tgtgccattt ggaacgcgtg cataaccctg atcggtaatt tgctgaccaa
      421 actcaacttt atcaaccaac tcgcgatttg ggtttagcaa ccatacacgt tcgcctgccg
      481 atgaaagttt gaaatttaaa tgcagtggcc cttgcgatcc atcttcatcg gcccaaagta
      541 ttaaatagtt atttggatta attatagtgc cttgaggcag ctcaaatttc tccagattca
      601 atgtatcatc ggtaataaaa tatccactga tgtcaaccgg gtttcccgat ttattataca
      661 actctatcca gtcatcatac tcaccggcat tgtccattgc agtattgctg ttacttgcca
      721 taacttcgtt tatggttata gttgtatcgg acgataacac cggagtaaca aaataaacat
      781 acacatcgtg ctcggcacct acaggactgt acattgctgt tttatacgaa tttccagctt
      841 ttgcttcaat gtaatatcgt acccattgcg aattgttttg tgccggaatg tatgccccat
      901 aaattccatc ggcagcactt gcatcgccat gcataccatc atcgtacata agtgaagaag
      961 taaaatttcc cacaataccg gtagcataaa ataatgttac tttatttatt ccattgccag
     1021 atgttactgt tgcattaaca cccacactat cggccgaagt aggataagtc caggttacac
     1081 tattggtagc aaatgaaaca tttgaaattg cagggcccgt ttgatttacc tctatattac
     1141 tcatgagata atttttacgg ttattcatcc atgtttttaa tgcagtattt tcggtgttga
     1201 acgctgtata ggagtatagt tttttagggt cattcaatac aacagaatcg atgaatgctt
     1261 tgttttgatt taaaaataaa ttgcacactg ccgtatcgaa ggactcggaa ataatagtgc
     1321 gtaaatgcgc aagataacgc tgacgccatt cgggcactgc atacaatctg ttcatgagcg
     1381 gatagtttac cttggtttcg ttataaaacg gactccagtt aagcgcactt atacgcatca
     1441 cactatttcc atcgtattct ataggtgtaa tgcgatgcgt ttcgggttca taatatacat
     1501 agtaatccat tttgcctttg aagatataac tgtcatcatc gctaaaggcg gtttcgcttg
     1561 caaggtgcca caatgcacga tccacatcca tactatcggg caacattgtt ggcaggttag
     1621 caagcgaagg cttattcaat ctgctgcaac cattgcgcaa gtcgttccat ggtgtagcgc
     1681 tgtttgtact tttcaattga tagtattgtt tgtacaatga agtatcgttg ccaagaaaat
     1741 tgagcgctgc cgtaccatcg ccccaccccg gattgccggg actaccggca ggtttatcgg
     1801 cacgccagtt ggttccgtta ttactcatga accactccgt gtaaaaatct ttattcagtt
     1861 gctgcacatt cggatacaag ccccagtttt gtccgttaat atacaagtgt acataggcac
     1921 actttgcaac aggtatatgc ttttttattt gattgagata aaaatactcg cgggtgaatg
     1981 atggatcatc gaaacaatta ttgaggttga gaatattgta accatcaata tcctgccccg
     2041 gtttgaaatt gtctgtggta atattaaacg atttcttttg agagttttga gtctgcgaat
     2101 aagatgtttg acccttgaaa cgcacaccca cactgtcgta cagcacacca tctaccgtca
     2161 tgccggcagc aagttcaatt ttgtttgcgt agttttggat aagcaatgtc caataatttg
     2221 cctgtggaaa attgagatac acctgcttga tttgggtttg gtcgtacaat ccattatcgg
     2281 gttttcctcc tgtatacaat gttcgcaaat cgggtgatag atacatttct tttggcagcc
     2341 cttgcgacat tgaggcttga tgtatgcata tagcaaatac tataatctgt aatgttttct
     2401 tcagcatatt aatttgatta tttttaattc ttgagaggca tatttgaaac taaagtttaa
     2461 tgtgaaagta atttttttct gcaaataata tccgtcatta ataaaataca tattgtatac
     2521 tcattaaaag
//
LOCUS       DAORQA010000253         2523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9434_length_2523_cov_568.440032, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000253 DAORQA010000000
VERSION     DAORQA010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9434_length_2523_cov_568.440032"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(326..1546)
                     /locus_tag="PK736_08720"
     CDS             complement(326..1546)
                     /locus_tag="PK736_08720"
                     /inference="COORDINATES: protein motif:HMM:NF033538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HRC33509.1"
                     /translation="MDSQQSLCERRQLLQQKIFSHPSISNFNVYSRAAFDKLSKCHTS
                     GIGVHQYKCNNAACAIVHQQYHSCGNRHCPNCGGMRREQWIQERMNELLPTTYFHVVF
                     TLPQELRSLAMGNMREMYSLLFDAAHYAINKLSADKKWMGAKPGIISVLHTWGQDVGY
                     HPHVHCIVTGGGISQEQMWVKEKRGNGKFLFPKTSITKIYKAYFVKQMQKRIAENKIK
                     IEDVPAHEAAINTADKKIWNVYAKAPFAGPAQVIEYIGRYTHKVAITAHRIQHIGDSD
                     ITFTYKDYNDGDKIKQMTLSHTEFTRRFEKHILPKRFVRIRHAGFLCHRNKTERLQRI
                     HTQLLLPPPPPRVTVPLHIQVMQRTGNDITICPVCKKGKLELMGSYKYINGKMINVND
                     LRNRGSPLKQNINR"
     gene            complement(1521..2096)
                     /locus_tag="PK736_08725"
     CDS             complement(1521..2096)
                     /locus_tag="PK736_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRC33510.1"
                     /translation="MTEDEVFKLISAPLTLKEYCVICLLYGCGMRISEVANLRIQDID
                     SVEKRIKVVLGKGGKDRFTLLPDDLLPNLRKYYVEAGRPAEYLFTSKQTKKAMHVRSM
                     QEVVNSGMAKAGFGDKSYTAHTLRHSFATHMLNNGSNLHVIKTLLGHSKIETTMVYLH
                     LQKHTQLGIQSPLDAMRQLHTPQDGFAAKSV"
     gene            complement(2162..2446)
                     /locus_tag="PK736_08730"
     CDS             complement(2162..2446)
                     /locus_tag="PK736_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33511.1"
                     /translation="MQKNEIGKKPLSADAQEWIEKMHRTMTIKEYGAGSVKSYVNEMT
                     LLFKHCNHLPVSSIRQETIEDYLLYIKEVHKVGRAKCRSVAQACSFFLSG"
BASE COUNT          603 a          549 c          529 g          842 t
ORIGIN      
        1 taacatttag ccgtatagac gaaagccccc cggcatcgta gcaaacgagg ggcttttgtc
       61 tatacggtgt tcagcgacac cggcatgggg tgtcgtcata attgagtggc tttgctggag
      121 gggggcgtgc taaattgctt aggtgtaata gtaaatcaat tttacttttt ggggcgttta
      181 actatgagtg tatacgttgg tgtattgcat tacttttgct tttagtcaat agtgccccta
      241 cccaaaattg agcgtacttt attaccatac agtttttttt cattgcgatt ccgattacca
      301 acggagtaag caatttttaa tgttttcatc gattaatgtt ttgtttaaga ggtgaacccc
      361 ggttgcgcaa gtcatttaca tttatcattt ttccatttat gtacttataa gagcccatta
      421 attctagctt tccctttttg caaacagggc aaatagtaat gtcgttacca gtgcgttgca
      481 ttacttgtat gtgcagtggc actgttactc tgggcggtgg tggtggcaat agtaactgtg
      541 tgtgtatacg ttgaagccgt tccgttttat tacgatggca aaggaaaccg gcatggcgta
      601 tgcgcacaaa gcgctttggc agtatatgct tctcaaacct tcgtgtaaac tcggtatggc
      661 ttagtgtcat ttgctttatc ttatcgccat cgttataatc tttgtaggtg aaggtgatgt
      721 cgctatcgcc aatgtgttgt atacgatgtg cagtaattgc tactttgtgt gtgtatcggc
      781 caatatattc tataacttgt gcagggcctg caaatggtgc tttggcatat acgttccata
      841 tttttttgtc ggcagtattt attgccgctt cgtgtgctgg cacatcttct atttttattt
      901 tattctctgc aatgcgcttt tgcatttgct ttacaaaata tgctttgtat atttttgtta
      961 tgctcgtttt tggaaataga aatttgccat tgccacgttt ttctttcacc cacatttgct
     1021 cctggcttat gccgccacct gttacaatgc agtgcacatg cgggtggtag cccacgtcct
     1081 gaccccaagt atgcagcaca ctgatgatgc ccggcttggc tcccatccat ttcttgtcgg
     1141 ccgataattt attaatcgca taatgtgcag catcaaaaag cagcgaatac atttcgcgca
     1201 tgttgcccat ggcaaggctg cgcaactctt gcggcagggt aaacactaca tgaaaatatg
     1261 tggtgggcag caactcattc atgcgctctt gtatccattg ctctctgcgc atgccaccac
     1321 agttagggca atgccggttg ccgcaactat ggtattgctg atgcactatt gcacatgcgg
     1381 cattgttgca tttatattga tgcacgccaa taccagaagt atgacacttg cttagtttat
     1441 caaatgccgc acgactatac acattgaagt tactgatact ggggtgtgaa aatatttttt
     1501 gttgcaacaa ctgccgcctc tcacacagac tttgctgcga atccatcttg tggtgtatgc
     1561 aattggcgca tagcatctaa cggtgactgt ataccaagct gcgtatgctt ttgtaaatgc
     1621 aaatacacca tcgttgtttc tattttgctg tgccccagca atgtctttat tacatgtaaa
     1681 ttgctgccat tgttgagcat gtgtgtagca aagctatgcc gcaaagtgtg cgctgtatat
     1741 gacttgtcgc caaagcctgc cttagccatg ccgctgttta ctacttcctg catgctgcgc
     1801 acatgcatgg cttttttagt ttgtttgctg gtaaataaat attcggcagg ccgtccggct
     1861 tctacataat atttgcgcaa gtttggcaac aagtcatcgg gcagcaaagt gaatcggtct
     1921 ttgccacctt tgccaagcac cacctttatg cgtttctcaa cactatcaat atcttgtatg
     1981 cgcaaattcg ccacttcact tatacgcata ccacagccat agagcaagca tattacacaa
     2041 tactctttta acgtgagtgg tgccgaaatt aatttgaaca cttcatcctc ggtcataatg
     2101 ttaggaagta taaattgttt tttgggatag agattgctcg gcactatgta gggcgaaggc
     2161 attacccgct taaaaaaaaa ctgcatgcct gagcaacact ccggcatttt gcccgcccta
     2221 ctttatgcac ctctttaatg tacaataaat aatcctctat ggtctcttgc ctgatactcg
     2281 ataccggtaa atgattgcaa tgcttaaata gcaacgtcat ttcatttaca taacttttta
     2341 cacttcctgc accatactcc ttgatggtca tggtgcggtg cattttttca atccactctt
     2401 gtgcatcagc actcaatggc tttttgccaa tttcattttt ttgcatttct ttactttttt
     2461 aaatttaagt atgcaaggta atggtatttg cgcgcagtag tcaccgcagg tgtcgcttga
     2521 aca
//
LOCUS       DAORQA010000254         2517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9464_length_2517_cov_3.984565, whole genome shotgun sequence.
ACCESSION   DAORQA010000254 DAORQA010000000
VERSION     DAORQA010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9464_length_2517_cov_3.984565"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..>1490
                     /locus_tag="PK736_08735"
     CDS             246..>1490
                     /locus_tag="PK736_08735"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRC33512.1"
                     /translation="MKKISLTILLFLTLVYASKAQSITVTGTVTSATDALPMPFVTVA
                     IKGTTNAVATNMDGKFSLSNVSATDSLLFSFVGYTQQVIVVGNQTAINVKMVPSSKQL
                     SEVTVTALGINRQKRAIGYSTQKIDGAEIQQSNTPNILSALSGKAAGVQIANADGVEG
                     GTTRITIRGNNSINGNNQPLIVVDGIPIDNDPGLTNVGRGRDWGSAINNINMVDVEDI
                     TILKGGAASALYGARGANGVVLITMKKGAKQKGIGVSYNMMYKNVHPSRYRDVQNTYG
                     GGAPNANVAIPKFELAPDGTPLFPILSTDPKFGYPGSSVSWGPKMEGQMIRWWNGEMA
                     PWEAQPDNLSIPFRDGKTITHNVSAEGGGEIGTMRVSLTRTKSTPIIYNSDYNQTAIN
                     TNSTINVSKKVSLGLSATYTDYN"
     assembly_gap    1491..1500
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1501..>2517
                     /locus_tag="PK736_08740"
     CDS             <1501..>2517
                     /locus_tag="PK736_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33513.1"
                     /translation="RLNSPMLGEDAASFNKGLLYSWPRSYRGEDLVNYQMKDGTRDPL
                     TGYPYQYIDQYLWWNNYNNNTTLHRSVLLGGISLAYKITDWLTFTGRTGIDFSNNMYE
                     TKNKPVDLIGLQNGYYNNEFLFSLSKKSFLKSNFDVNFNLGGSSWSRNLHGISAHSGT
                     WYYPNWYSLSNFTPTTYGTDANGNVTVITKGDDPASLIAANTFYKKKINSFYGFLNVG
                     FKEYLFVELTGRNDFTSTLPSDANSYFYPGMSVSYIASEALKFNKDWFSFCKIRAGAS
                     QTATDTDPYQTEFYYNTSLHGGQQSSNYPSVIPPITLKPQRVNSYEAGTNLGFFKDVM
                     TVDFT"
BASE COUNT          802 a          494 c          494 g          717 t
ORIGIN      
        1 gtattaaaac aatctattct aatccaccca tccgtttctt tatagaaacc cacattccct
       61 tttttctttt tttgactaat tgcgtaaaac gaaatagagc aatatttttt gtttagaaga
      121 tggaaccctg ttttaatttt ttcaggatgc tgaattatca aacagaaaaa tcaatatttg
      181 aagcaagaat atttttacat tgcgtcacct tttgctgagt tcttcacatt gaattttatt
      241 ttattatgaa aaaaatttct ctcacaatac ttttatttct cacgttagtt tatgcttcca
      301 aagcacaaag cattacggtt acaggcacag ttacatctgc taccgatgcc ttgcctatgc
      361 cctttgtaac tgtagcaatc aaaggcacta caaatgctgt ggcaacaaat atggacggaa
      421 agttttcgtt gtcgaatgtg agtgctaccg attcgttact ttttagtttt gttggatata
      481 cgcagcaagt aattgttgtt ggtaatcaaa ctgctatcaa cgtaaaaatg gtaccaagtt
      541 caaaacaatt gagtgaagtt acagttaccg cactaggcat caaccggcaa aaacgcgcca
      601 taggatattc gacacaaaaa atagatggtg ccgaaattca acaatccaat acaccaaata
      661 ttctaagtgc attatcgggc aaagctgccg gtgtgcaaat agcaaatgcc gatggtgtgg
      721 aaggtggtac aacacgaata acaataagag gcaacaacag tataaatggc aacaaccaac
      781 cacttattgt ggtagatggt attcctatcg acaacgaccc gggacttacc aatgtggggc
      841 gtgggcgtga ttggggctcg gccatcaaca atattaatat ggtcgatgta gaagacatta
      901 ccatacttaa aggcggtgca gcctcagcat tatacggtgc acgcggtgct aacggtgtag
      961 ttttgataac gatgaaaaaa ggcgccaaac aaaaagggat tggcgtatcg tataatatga
     1021 tgtataaaaa tgtgcatcct tcccgctatc gcgatgtgca aaatacttat ggcggaggtg
     1081 ctccaaatgc aaacgtggct attccaaaat ttgaattggc gcccgatggc acacctttgt
     1141 ttcccatttt atctaccgat ccaaagtttg gctacccggg ctcatcagtt tcgtggggac
     1201 caaaaatgga agggcaaatg attcgttggt ggaatggtga aatggcacca tgggaagcac
     1261 aacccgataa tttatctata ccatttagag atggaaaaac aattacacac aatgtgtcgg
     1321 cagagggcgg aggcgaaata ggcacaatgc gtgtttcact tacaagaaca aaaagcacac
     1381 ccataattta caacagcgat tacaatcaaa cggctatcaa taccaattca acaataaatg
     1441 tgtcgaaaaa agtaagcctt ggcttgtcgg ccacatatac agattacaac nnnnnnnnnn
     1501 aggcttaaca gcccaatgct tggcgaagat gctgcatcat tcaacaaagg attattatac
     1561 tcatggccgc gtagttatcg tggcgaagat ttggtaaatt atcaaatgaa agatggtacg
     1621 cgcgatccgc ttacaggtta tccttatcaa tatatcgatc aatatttatg gtggaataat
     1681 tacaacaaca atactacact acaccgttct gttttattgg ggggtatttc gctggcatat
     1741 aaaattacgg actggttaac ctttacaggc cgaaccggaa tcgacttttc gaacaatatg
     1801 tatgaaacaa aaaacaaacc ggtcgatctt atcgggttgc aaaatggata ttacaacaac
     1861 gaatttttat tttcgctttc gaagaaatca tttttgaaat cgaattttga tgtgaatttt
     1921 aatctcggtg gttcgagctg gtcgcgcaac ttacatggca taagcgcaca ttcgggcacc
     1981 tggtattatc caaactggta cagcttatcg aacttcactc ctaccactta tggcaccgat
     2041 gcaaatggaa atgttaccgt aataactaaa ggtgacgacc ctgcatcatt aatagcagcg
     2101 aatacttttt ataagaaaaa aataaactct ttttatggat ttttaaatgt tggctttaaa
     2161 gaatatttat ttgttgagct taccggccgt aacgatttta catcaacttt gccatcggat
     2221 gccaactcgt atttttatcc cggaatgtcg gtaagctata ttgcctccga agcattaaaa
     2281 tttaataaag actggtttag cttttgtaaa atacgtgcag gtgcatcgca aacagccacc
     2341 gataccgatc cgtatcaaac agaattttac tacaacactt cgttgcatgg tggtcagcag
     2401 tcatcaaatt acccatcggt aatacctcca ataacattaa agccacagcg ggtaaattcg
     2461 tatgaggcag gtacaaatct tggatttttt aaagacgtga tgacggttga ttttact
//
LOCUS       DAORQA010000255         2509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9495_length_2509_cov_5.916055, whole genome shotgun sequence.
ACCESSION   DAORQA010000255 DAORQA010000000
VERSION     DAORQA010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9495_length_2509_cov_5.916055"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2486)
                     /locus_tag="PK736_08745"
     CDS             complement(<1..2486)
                     /locus_tag="PK736_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33514.1"
                     /translation="MNPSDANCSNVCIELGTKNGINHFIFNNIFSNKTPNQTSPLYHS
                     IYYSPGNYSMGTGGFNYNSFYFENNTGAFFGVTPIGNFPSKADWDGNITGPGPEQQTI
                     ECNPQFASDKNDLHTANLSLNSTGTTPSVWITTDIDCNARTAPHDIGATLITTCNGTP
                     TAGTAVASSLNPCAKSNVQLSLIGNNFGPGISFQWQESLNGTIGTFSNIAGATNNAYQ
                     IDSISMDKFYRCYIICNSSGIKDSTNILNILVRALPSVNIIPSTASICQPNASAQPLT
                     AFGGATYSWAPAIGLSSTVNKQTLASPSVSTTYTVTATATNGCTKSVICPVIVSRRIY
                     INDATATPTQVCSGGSSQLSIAAGTTSNYAVQQMPYTNYIGTKGKIIFPINTNNDYKE
                     INLPSKFLFDFFGKTYDKLFIQVNGFVSFGAGSTFKNAQTLPNSNKPNNVIALCQSDL
                     LLSTGSVDTFTIGTFPNRKFVISYDSLRFYINGNPSGYIDGQLVLLETQNIIESHLAY
                     VSNGTISVRSKTMGIENETGTVGIAPPSRNDQNFQITTAEGWRYVPSGGNPTYSWSPA
                     TNIVNGTTSNPTATNITANTTYIATATESGGCKTQKLFNITVSNINAAIVNTANYVCE
                     GNSILINAGASSGIPPYNYLWSNGSIAQSLNLTLVGTSSYTVTVTDAIGCTRSASQKI
                     TFVPSPQVSLSSNSPVCAGTSLIVNGSGALNFDWTGPQGFATNTSDFEIQNIDVANAG
                     DYIVTCTDSLGCSTEEVLPISVLQNDMPLAGSNSPVIAGDSIFLNCTVADSYFWEGPE
                     GFTSTLQNPVLENASQNLAGTYTVTVTKANG"
BASE COUNT          713 a          471 c          541 g          784 t
ORIGIN      
        1 ccatttgctt ttgtgacagt aacagtatac gttcctgcta aattttgtga agcattttcc
       61 aatactggat tttgtaacgt tgaagtaaaa ccctcaggcc cttcccaaaa ataactatca
      121 gctacggtac aatttaggaa aatactatcg cctgcaataa cagggctgtt gctgcccgca
      181 agcggcatgt cgttttgcaa aaccgatatt ggtaaaactt cctcggtgct gcaacctaaa
      241 ctatctgtgc aagtaacaat gtagtcacct gcattggcaa cgtcaatatt ttgaatttcg
      301 aagtccgaag tgttggtcgc aaaaccttgt ggccctgtcc aatcaaagtt taaagccccg
      361 ctaccattta caatgagcga agtgccggca cataccggac tatttgaact aagtgatacc
      421 tgaggggacg gcacaaaagt aattttctgt gaagcagacc tggtacaacc aatggcatca
      481 gtaacagtaa ctgtatacga tgaagtacca accaaagtta aatttaatga ttgagctatt
      541 gatccattgc tccacaagta attgtatggc ggtattccgg aacttgctcc ggcatttatt
      601 aatatgctat tgccttcgca aacgtaattg gctgtattta caatagctgc atttatattg
      661 ctcacggtaa tattgaatag cttttgcgtt ttacaacctc cactttcagt tgcagttgca
      721 atgtatgtgg tattagctgt aatgttagtg gcagtaggat tcgatgtagt gccattaaca
      781 atattagttg ccggtgacca tgagtaggtt ggattaccac ccgaaggaac atatctccac
      841 ccttcggcag tagtgatttg aaaattttga tcgttgcgcg atggtggtgc tatgcctaca
      901 gtgcccgttt cattttcaat acccatagtt ttggaacgta ctgatattgt accattgcta
      961 acatatgcaa ggtgactctc tataatattt tgggtttcga gcaaaaccaa ttggccatcg
     1021 atataacccg aagggtttcc attgatgtag aaacgaaggc tatcatagct gataacaaat
     1081 ttacgattgg gaaatgttcc aatggtaaaa gtatcgaccg aacctgttga taataacaaa
     1141 tcagactgac ataaggcaat cacattgttg ggcttgttac tgtttggaag tgtttgtgca
     1201 tttttaaatg ttgaacctgc gccaaaagaa acaaagccgt taacctgaat aaaaagcttg
     1261 tcgtatgttt ttccaaaaaa atcgaaaagg aatttcgagg gcaaattgat ctctttgtaa
     1321 tcgttattag tatttattgg aaagataatt tttcccttag taccaatgta gttagtgtaa
     1381 ggcatttgtt gcacagcata gttggaagtt gtaccggctg caatgctgag ttgtgatgag
     1441 ccgccactgc atacttgtgt tggagttgca gtagcatcgt taatgtaaat tctgcggctt
     1501 actataacag ggcaaattac cgatttggtg cacccatttg tagctgtagc ggtaacagta
     1561 tacgtagtac taaccgatgg agaagcaagt gtttgcttat ttacggtgct cgacaatcct
     1621 attgcggggg cccacgaata tgttgcgcca ccaaaagctg tcaatggttg tgctgaggca
     1681 tttggctgac aaatggaagc tgtgcttggt atgatgttta cacttggcaa tgcccttacc
     1741 agtatattca aaatatttgt agagtctttt attccggatg aattgcagat aatataacag
     1801 cgataaaatt tatccattga tattgaatca atctggtaag cattgtttgt agcgcctgca
     1861 atatttgaga atgttcctat tgttccattc agcgactctt gccactgaaa tgaaatgccg
     1921 gggccaaaat tatttccgat aagtgaaagc tgtacattac tttttgcgca agggtttaaa
     1981 ctgctggcaa cggcagttcc tgctgtggga gttccattac aggtagtaat taatgtagca
     2041 ccaatatcgt gcggtgccgt gcgcgcattg caatctatgt cggttgtaat ccaaaccgaa
     2101 ggtgttgttc ctgttgaatt aagactcagg ttagcagtgt gtaaatcgtt tttatcactt
     2161 gcaaactgcg gattgcattc tatagtttgt tgctcgggcc cggggcctgt aatgttacca
     2221 tcccaatcgg ccttagaagg gaaatttcca atgggcgtaa cgccaaaaaa tgcgcctgta
     2281 ttattttcga aataaaagct gttgtaatta aatcctcctg ttcccatgct ataatttccg
     2341 ggcgaataat aaattgaatg atacagtggg cttgtttggt tcggtgtttt attcgaaaaa
     2401 atattattga aaataaaatg atttatgcca ttcttagttc caagttctat acacacgttt
     2461 gagcagttgg catcggatgg gttcaaatta atattattat gttcaatat
//
LOCUS       DAORQA010000256         2491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9574_length_2491_cov_15.024631, whole genome shotgun sequence.
ACCESSION   DAORQA010000256 DAORQA010000000
VERSION     DAORQA010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9574_length_2491_cov_15.024631"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="PK736_08750"
     CDS             complement(<1..1020)
                     /locus_tag="PK736_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRC33515.1"
                     /translation="MVIKKERKIFVIDTSVILYDHNVINNFKEHDVAIPITVLEEVDR
                     FKKGNDVINYEAREFIRALDKLSGAYGLTEWIPLNGPTKGKLKVQMTEHGARIDANIV
                     FGEKKADHNIINAALVLAETEPKRKVVLVSKDINLRLKAKSLNIFSEDYQTGKVQDIE
                     GLYTGKTIVPNLARKIIDTIYQVGYCPRKTIFKNKPVANHYYILQSKEGDTSALAFYN
                     PGTDCIERIQKESVSGIKPRNAEQAFALHAIMNPDVKLVTLQGVAGTGKTLIALASAL
                     EQKSKYRQIFLARPIVPLSNKDIGYLPGDIKSKINPYMEPLWDNLKFIQNQYAETDKE
                     FKKIND"
     gene            1240..>2491
                     /locus_tag="PK736_08755"
     CDS             1240..>2491
                     /locus_tag="PK736_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRC33516.1"
                     /translation="MVKIKGYIFCICVLLYCSLISAQQTIVTKPVHDGSQITQYSYVQ
                     SYELMLKDYQQLPQLKFWCDVVQCVSDSSIINIASSRTGLYKVCTAQWQLQTEAQKLV
                     YKDSVKKFFQIDSLSSLYVTAGKREFYEFKKAAPLISDGIKAFKKIGVDPWYAQAILL
                     IESPGKLHAVSSAGARGPFQLMPEVARAFGLKVNRKVDERTNFERSAMAASKFIKVNC
                     IPFSKRLCESSGITYNENDLWFKLLTMHIYNAGFGNVKAAVDKLCPGTGGVELIKGLW
                     QTEAAQFKNQSQNYSQIVLACMNQYQKMMHQSDTIFLSDGDALMTAASKLPPALKVIT
                     LKKCIKAYELDLIDGIITADQFKGKLECIQNEMQYYSKAEASTITAMDLNRVGNKLMS
                     KRNMKEAIEIFKYNENLYPENKMVY"
BASE COUNT          814 a          440 c          469 g          768 t
ORIGIN      
        1 gtcattaatt ttcttaaatt ctttatcggt ttcagcatat tggttttgta taaatttcaa
       61 attatcccaa agtggttcca tgtaaggatt gattttcgat tttatatctc cgggcaaata
      121 tccaatgtct ttattactta gtggaacaat agggcgagct aaaaatattt ggcggtactt
      181 gcttttttgt tccaatgccg atgcaagtgc tatcaatgtt ttgccggtac cagccacacc
      241 ttgtaaagtc acaagtttaa catcgggatt cataatagca tgcagggcaa aagcttgttc
      301 agcattacga ggctttatgc cgcttaccga ttctttttga atacgctcta tacaatctgt
      361 accaggatta taaaatgcaa gtgccgaagt atcaccttct ttagattgta gtatatagta
      421 atggttagct acaggtttgt ttttaaaaat agttttacgg ggacagtaac ccacctgata
      481 aatagtatca ataattttac gcgcaagatt gggaacaata gttttgcctg tgtaaagacc
      541 ttctatgtcc tgcacttttc cggtttgata atcttccgaa aaaatattca gtgatttggc
      601 tttcaatctt aggtttatat ccttcgatac caaaaccact tttcgcttgg gctcagtttc
      661 ggcaagcacc aaggctgcat tgataatatt atgatctgcc tttttttctc cgaacacaat
      721 gtttgcatcg atgcgtgcgc catgctcggt catttgcact tttaactttc cttttgtagg
      781 accattgagt ggtatccatt cggtaaggcc atatgcacca ctcaatttat cgagggcacg
      841 gataaactca cgtgcttcgt aattgataac atcatttcct tttttaaaac gatcaacctc
      901 ttcgagaacc gttattggaa tagcgacatc atgttccttg aaattattta taacattatg
      961 gtcgtataga attaccgatg tatcgataac gaaaattttt ctttcttttt taattaccat
     1021 ttgtaaaaag tttgaaacaa aaatagattc acaacctatc cttattcatt attaaattgt
     1081 gttctatgaa cataacgatg ttagtaaaac aaaaagtgtt aattaatgaa atctttacaa
     1141 aactattttt agatttgttg catcgaataa ttcccgcata ataaaaggaa aacaaaagcg
     1201 taattaaatt aaagtttaaa ggaagtacat caatagagaa tggtaaaaat aaagggctat
     1261 atattttgta tttgtgtcct gctatattgc agtctaatat cagcgcaaca aaccatagtt
     1321 acaaaacctg tgcacgatgg ttcgcaaatt acccaatatt cgtatgtgca atcttacgag
     1381 ttgatgttga aagattacca acagttgcca caattaaaat tttggtgcga tgtagtgcaa
     1441 tgtgtgagcg actcgagtat tataaatatt gcatcgagcc gaaccgggtt gtataaagta
     1501 tgcactgcac aatggcaatt gcaaacagag gcacaaaaat tagtttacaa agatagtgta
     1561 aagaaatttt ttcaaattga ttcactttcg agtttgtatg ttacagcagg caagcgcgag
     1621 ttttacgaat ttaaaaaagc tgcaccttta attagtgatg gaataaaagc tttcaaaaag
     1681 atcggtgtcg acccttggta cgcgcaggca atattactta tcgaaagtcc cggcaaattg
     1741 catgcagtat caagcgcagg cgcaagagga ccatttcagt tgatgcccga ggtagccagg
     1801 gcattcggat taaaagtaaa ccgcaaagta gatgaacgca caaactttga gcgcagtgca
     1861 atggcagcat ctaaatttat aaaagtaaat tgcattccat tctcaaaaag attatgcgaa
     1921 tcatcgggca ttacttacaa tgaaaatgat ttgtggttta agctattaac aatgcatata
     1981 tacaatgccg gttttgggaa tgtaaaagcc gctgtcgaca aattgtgtcc gggcacagga
     2041 ggtgttgaat taataaaagg cctgtggcaa acagaagccg cgcagttcaa aaaccaatcg
     2101 cagaactatt ctcaaattgt tttggcttgc atgaatcagt atcaaaaaat gatgcatcaa
     2161 agtgatacaa tatttcttag tgatggcgat gctttgatga ctgcagcaag taaattgcct
     2221 ccggcactga aagtaattac acttaaaaaa tgcatcaagg cttatgagtt ggatttgatt
     2281 gatggcatta tcactgccga ccagttcaaa ggtaaattag aatgcataca aaacgaaatg
     2341 caatattact caaaggccga agcttcaacc attactgcta tggacctgaa cagagttggc
     2401 aataaattga tgagtaaacg taacatgaaa gaagccatcg aaatttttaa gtacaacgaa
     2461 aatctatacc ccgaaaataa aatggtatac g
//
LOCUS       DAORQA010000257         2489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9583_length_2489_cov_9.691044, whole genome shotgun sequence.
ACCESSION   DAORQA010000257 DAORQA010000000
VERSION     DAORQA010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9583_length_2489_cov_9.691044"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..2085
                     /locus_tag="PK736_08760"
     CDS             22..2085
                     /locus_tag="PK736_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRC33517.1"
                     /translation="MYAPNTLNINDSIELTVYYHGKPLTDASGWGGFYFSTGYVFNLG
                     VGFAADPHTFGRAWFPCVDEFTDKATYDFYIATEPNKKAFCNGILIDSSLQANTNNLI
                     WHWKMNQPIPTYLACMAVSSYYTLEQNINGIKAQFATSILDTTKVKNTFVHLPDIMTT
                     YINSFGPYPFDKIGFSMVPFGSGAMEHASQITIGSAFIDGSLTYESLWAHELSHMWWG
                     DKVTCETAGDMWLNEGWASFNEAYTREFLYGKQSYKNYVSANHKTVLQFAHVDDGGYL
                     SFINVPHAYTYGSTVYKKGADAAHTLRNFLGDSLFKIGMQFYMNNRAYGNANSYQFRD
                     DIIASTGVDVTRFFDDWIFTEGFPHFSIDSVVFVPGGLDHYFVYTRQRSKGNNHLYSM
                     PVDITLSNGTTDTTYTIVIDSATQMFHIATLGVFDWFALDRSEKISDAITDDEITVNA
                     VATTNLPETFTSINSIAVPVSGSLVRVEHNWVAPDGFKNSNPGIRLSNYRYWTINGHF
                     KPGYHAKATFTYDGRTIANTGHLDNTLGIIIEDSLVLVYRNGSGDDWKIIPQTTNFNG
                     SHVDRFGTITIDSLMRGEYALGYYDFTVGVPSISQAAKTYTLTVSPNPTNKICNIAFT
                     LNNLKQATIAITNMLGAVVKVYTVYAHQEQVSWDAADMPAGTYNICLTSGDKILNSEK
                     LILTK"
BASE COUNT          786 a          488 c          483 g          732 t
ORIGIN      
        1 taaaatgata caaccataag tatatacgca cccaatacat taaatattaa cgacagcatt
       61 gagcttacag tttactatca tggcaagcca cttaccgatg catcgggatg gggagggttt
      121 tacttttcaa ccggttatgt tttcaatctt ggtgtaggtt ttgctgccga cccacatacg
      181 tttggccgtg catggtttcc atgtgtggat gaatttacag acaaggccac ttacgatttt
      241 tatattgcca ccgagccaaa taaaaaagca ttttgcaacg gtattcttat cgacagttcg
      301 ctgcaggcca acacaaataa tttaatatgg cattggaaaa tgaatcaacc tataccaacc
      361 tatttagcat gtatggctgt ttcttcatac tacacattag agcaaaatat aaatggaata
      421 aaagcacagt ttgcaacatc tatactcgat acaacaaaag taaaaaacac cttcgtgcat
      481 ttgcctgata ttatgaccac atacatcaac tcttttgggc cttatccttt cgataaaatt
      541 ggattcagca tggtgccctt tggttcaggt gctatggaac atgcatcgca aattactatt
      601 ggctctgcgt ttattgatgg ttcacttact tacgaatcgt tatgggcgca tgaactatcg
      661 catatgtggt ggggcgataa agttacatgc gaaacggcag gcgatatgtg gctcaacgaa
      721 ggatgggcat cttttaacga agcatacacg cgcgaatttt tatatggcaa acaatcttac
      781 aaaaattatg tatcggccaa tcataaaact gttttgcaat ttgctcatgt ggatgatggg
      841 ggatatttat cattcatcaa tgtgcctcat gcatatacat atggaagtac agtttataaa
      901 aaaggggctg atgctgcgca cacccttcgc aattttttgg gtgatagtct ttttaaaatt
      961 ggcatgcaat tttatatgaa taaccgtgct tatggcaatg caaattcata tcagtttcgc
     1021 gatgatatta ttgcatcaac cggagtagat gttacacgtt tttttgatga ctggattttt
     1081 accgaaggct ttccgcattt cagtatcgac tccgttgtat ttgttcctgg tggtctcgat
     1141 cattattttg tttatacacg gcagcgcagc aaaggcaaca atcacctgta ctctatgccc
     1201 gtggatatta cgcttagcaa tggcactacc gacacaactt atactatagt gatagacagc
     1261 gcaacacaaa tgtttcatat tgccaccctg ggtgtattcg attggtttgc actcgaccgt
     1321 agcgaaaaaa taagcgatgc cataaccgat gatgagatta cagttaatgc agtggctaca
     1381 accaatttgc ccgaaacatt tacatcaata aattctattg ctgttcctgt tagcggatcg
     1441 cttgtacgtg tagaacacaa ttgggtagca cccgatggtt ttaagaatag taatcccgga
     1501 atacgtttat ctaattatag atactggacc atcaatggcc actttaaacc cggttaccac
     1561 gcaaaggcaa ctttcactta cgatggccga acaatagcaa ataccgggca tctcgataat
     1621 acattgggaa ttattatcga agacagttta gtgttggttt accgaaatgg cagtggcgat
     1681 gattggaaaa taatacctca aactacaaac ttcaacggct cgcatgtaga caggtttggt
     1741 acaataacca tcgactcgct catgcgcggt gaatatgcac tgggatacta tgattttact
     1801 gttggtgtgc cctcaatttc acaggcagcc aaaacgtata cccttacagt tagccctaac
     1861 ccaacaaata aaatttgtaa catcgcattc acgcttaata atttgaagca agcaactata
     1921 gcaataacta acatgctggg tgctgttgta aaagtctaca ccgtttatgc acatcaggag
     1981 caagtatcgt gggatgctgc cgatatgccg gcaggcacat ataatatctg cctcaccagt
     2041 ggcgacaaaa ttcttaatag cgaaaagctt attcttacta agtaagataa agcattgccg
     2101 ttgcatcaat tagtttaata aacttattta ttgaaggagg ttaatggaac ttagatgttc
     2161 atattgcctg aaagcaacta cggttacttt taaaaagctc tgttgttact tttaaaaatt
     2221 aaaaactcca tcatagaatt gaatatatga acacagtaaa tatatttgta gcaaaacctt
     2281 cctattgcta aacacattgg acagattgga gaaagttaag gattaataaa ttctccaagc
     2341 tgctagttat gcaccatatt agacgacaga aatgactgac ataaaagaaa tagcagacga
     2401 catttatagc aaagagaaaa caaaatctca tcgcgacctt atcgacaatt ttgagcggca
     2461 tcaaaagagt tgatgtatgt ggtcataat
//
LOCUS       DAORQA010000258         2482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9613_length_2482_cov_24.082406, whole genome shotgun sequence.
ACCESSION   DAORQA010000258 DAORQA010000000
VERSION     DAORQA010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9613_length_2482_cov_24.082406"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PK736_08765"
     CDS             <1..421
                     /locus_tag="PK736_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33518.1"
                     /translation="NPHVVSTLLTGLDPNTVYNYNIKPNCGSSTNQSITYNFTTLANK
                     PLSKLFWDDIVSSSLINIYPNPNSGRFTMQFESDIVQEGRVQIIDVSGRIVYTSNIDI
                     IEGMNEMKIDVTNVTAGVYAAQLIIAGETITTKMIIE"
     gene            complement(519..1613)
                     /gene="hppD"
                     /locus_tag="PK736_08770"
     CDS             complement(519..1613)
                     /gene="hppD"
                     /locus_tag="PK736_08770"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRC33519.1"
                     /translation="MSEDFLPLNGTDHIEFYCGNAKQSAYFYQHAFGFELVAYSGPET
                     GVRDRASYVLQQNKIRFVLTTALSPDHEITHHVAKHGDGVKVLALWVDDAEKAFYETM
                     ARGAKAHTQPRVITDEYGEVKIAAIHTYGETIHKFIERKNYSGPFLPGYKAAKSKFKI
                     SPIGLEVVDHCVGNVELGKMNDWVKFYEDVMGFKMIITFDDKDISTEYSALMSKVVSN
                     GNGFVKFPINEPAEGKKKSQIDEYLEFYNGAGVQHMALLTNDVIGTVTELQSRGIDFL
                     TVPTTYYDELEGRVGKIDEDVATLKKLGILVDRDDEGYLLQIFTKPVEDRPTVFFEII
                     QRKGAKSFGKGNFKALFEAIEREQAIRGNL"
     gene            complement(1657..2163)
                     /locus_tag="PK736_08775"
     CDS             complement(1657..2163)
                     /locus_tag="PK736_08775"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HRC33520.1"
                     /translation="MIQFQKHFLLIFSFCFSLLVSCKVKDNNSVSKSVSNDSLFLSID
                     KSPCFGACPTYTMVIYSNGGKSTLSGKKFTKNLGKFEYVFSNDQIKDLKVKITEADFF
                     NLKDSYDDERVMDLPMSTIDVFMGGVRKKVVGRYETPKEFRVFETYLDSLASLPVWQK
                     VKPPVGDD"
     gene            complement(2169..>2482)
                     /locus_tag="PK736_08780"
     CDS             complement(2169..>2482)
                     /locus_tag="PK736_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRC33521.1"
                     /translation="FTYFNSQQLNTAANPAMKWMMYLMPVLFLGFLNDFPAGLNYYYF
                     LGNIISIGTQLLFKSFVDQDKIHLQIQENKKKPDTKKKSSFQERLEKMAKERGYQPPK
                     K"
BASE COUNT          763 a          471 c          434 g          814 t
ORIGIN      
        1 taatccacat gtagtaagca ctttacttac aggccttgat cccaatactg tttacaatta
       61 caacataaaa ccaaactgtg gttcttcaac aaatcagagt atcacttata atttcacaac
      121 gcttgcaaat aagccattga gtaaattgtt ttgggatgat attgtatcaa gttcattgat
      181 taatatttat ccgaatccaa attcggggag atttacaatg cagttcgaaa gtgatattgt
      241 gcaagaaggc agagtacaaa ttatagatgt ttcgggacga atagtttata catcaaatat
      301 tgacataata gaaggaatga atgaaatgaa gattgatgtt acaaatgtaa cggcaggtgt
      361 atacgctgct cagttaatta ttgccggaga aacaataaca acgaaaatga taattgaata
      421 aatatcgata agaaccagag aaaagggaaa aagagaaaag ggtgctcttt accgccaaca
      481 cttaacatgt tggcggtttt tttttgagct tttgtttttt ataaattacc tcgaatggct
      541 tgttcgcgct cgatagcctc gaacagtgcc ttgaagtttc ctttgccaaa agatttagcg
      601 cctttgcgct gaataatttc gaaaaatact gttggacgat cttctacagg ctttgtaaaa
      661 atttgaagta aatatccttc gtcatcacga tctacaagta ttccaagttt ttttaatgtg
      721 gcaacatctt cgtctatttt gcctacacgt ccttctaact catcatagta tgtagtaggt
      781 acagttaaaa agtcaatgcc acgcgattgc aattcggtaa cggtgccaat aacatcgttt
      841 gttaataagg ccatgtgctg cacaccggca ccattataaa attcgaggta ctcgtcaatt
      901 tgtgatttct tttttccttc ggcaggttcg tttatgggaa attttacaaa tccatttccg
      961 ttagaaacaa ctttactcat caacgctgaa tattcagttg agatatcttt gtcatcaaaa
     1021 gtgattatca ttttgaagcc cattacatct tcgtaaaact tcacccaatc gttcatcttt
     1081 ccaagttcca catttccaac acagtgatca accacttcta aaccaattgg agaaattttg
     1141 aacttcgact tagctgcttt gtaccccggt agaaatggcc ctgaataatt tttgcgctct
     1201 ataaatttgt gaatggtttc gccatatgta tggatggcag caatttttac ttcgccatat
     1261 tcgtcagtaa taacacgtgg ttgagtatgt gcttttgcac cgcgtgccat agtttcgtaa
     1321 aatgcttttt cggcatcatc aacccaaagt gccaatacct ttactccatc gccatgttta
     1381 gccacatgat gtgtaatttc atgatcgggc gatagagctg ttgtgagcac aaaacgaatc
     1441 ttgttttgtt gcaacacata cgaagcgcgg tcgcgtacac cagtttcggg acctgagtat
     1501 gctaccaatt cgaacccgaa ggcatgttga tagaaatagg ccgattgctt ggcattgcca
     1561 cagtagaatt caatatggtc ggtgccgttt aatggtaaaa aatcttctga catggtatta
     1621 tttattttaa gctattacaa aattatagaa atatttttaa tcatcaccta ccggtggttt
     1681 tactttttgc cacacaggca atgatgccag gctatcgaga tatgtttcga atactcgaaa
     1741 ttcttttggg gtttcgtacc tgccaacaac ttttttacgg actccaccca tgaatacatc
     1801 tatagttgac ataggtaaat ccattactct ctcgtcatca taactgtctt tcaagttaaa
     1861 aaaatcagcc tcagtaatct ttactttcag atctttgatt tggtcatttg aaaaaacgta
     1921 ttcaaatttc cctaagtttt tagtaaactt tttacccgat aatgttgact taccaccgtt
     1981 tgaataaatt accatagtgt aagtaggaca agcaccaaaa caggggcttt tatcaatact
     2041 caaaaacaat gaatcgttgc ttacgctttt acttacgcta ttattatctt tcaccttgca
     2101 ggaaaccaat aatgaaaaac aaaaagaaaa tatcagcaag aaatgttttt gaaactgaat
     2161 cattcgtgtt atttttttgg tggttgataa ccgcgctcct ttgccatttt ttctaacctt
     2221 tcctgaaaac ttgatttctt ttttgtgtcg ggtttctttt tgttttcctg tatctgcaaa
     2281 tgtattttat cctgatcaac aaacgatttg aaaagcaatt gtgtgcctat gctaataata
     2341 tttccaagga aataatagta attcaatcct gcaggaaaat catttaagaa acctaagaat
     2401 aataccggca taagatacat catccatttc atagccgggt tagcagcggt attcaattgt
     2461 tgcgagttga agtaggtaaa ca
//
LOCUS       DAORQA010000259         2480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9629_length_2480_cov_5.530309, whole genome shotgun sequence.
ACCESSION   DAORQA010000259 DAORQA010000000
VERSION     DAORQA010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9629_length_2480_cov_5.530309"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="PK736_08785"
     CDS             <1..905
                     /locus_tag="PK736_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33522.1"
                     /translation="VGITFQWYKGNIPISGATSPLYYAKSAGTYKVIVASPPCSSTSP
                     NISLTENSLPTSTITVNGLSTFCYGDSTQLSANAGVGFAYQWRKNGVNIPGATSKNLV
                     VKTTGNYRVIVTNANGCKATSATKTITVKPLPTATITALGPLSFCNGDSVKLVANSLV
                     GATYKWKKYSNFIANATSINYTAKTSGVYKAQITDINGCSKVSNTLTVNVNCREQNDL
                     AAQSNILIEPNPFNENTTITWPGYFGKNIDIKIYNTTGSLMKDESIKQTDNSAKITIK
                     PAGLYFVVITTANEAIVKRIMKVD"
     gene            complement(1051..1323)
                     /locus_tag="PK736_08790"
     CDS             complement(1051..1323)
                     /locus_tag="PK736_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33523.1"
                     /translation="MKKKFLVAIISFLMAGVAFAQDQTKPVVKAAQATATKKAEQAKA
                     VAQDAAKPTVKAAEAKVKKVKTDAQKALKKVKLKKHHKGQKASGKK"
     gene            complement(1562..2308)
                     /gene="fabG"
                     /locus_tag="PK736_08795"
     CDS             complement(1562..2308)
                     /gene="fabG"
                     /locus_tag="PK736_08795"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015782609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRC33524.1"
                     /translation="MKLLAGKVALITGASRGIGRGIALALAEQGANIAFTYNSSTEQA
                     MLLQDEIKHFGVTGIGFQSNAADYTAAENLVAGVLAQFPTIDIVVNNAGITRDNLLMR
                     MSEQHFDEVINTNLKSVFNITKAVQRQLLKQRSGSIINISSVVGVKGNAGQANYAASK
                     AGIIGFTKSIALELGSRNIRCNAIAPGFIETEMTAALNQETVKQWREAIPLKRGGTPQ
                     DVANLVVFLASDNSTYITGQVINVDGGMLT"
BASE COUNT          742 a          456 c          465 g          817 t
ORIGIN      
        1 gtgtaggcat tacatttcaa tggtataaag gtaatattcc tataagtggt gctacttcac
       61 cgttgtatta tgcaaaaagt gcaggtacgt ataaggtgat agttgcatcg ccaccttgct
      121 cgtccacatc tcctaacata agtttaaccg aaaactcttt gccaacatca acaattactg
      181 tgaatggtct ttccacattt tgttatggtg acagtacgca gctttcggca aatgccggtg
      241 taggttttgc atatcagtgg agaaaaaatg gtgtaaatat tccaggtgcc acaagcaaaa
      301 accttgtagt gaaaacaaca ggaaactacc gggttatagt aaccaatgca aacggttgta
      361 aagccacctc ggccacaaaa accattacag taaaaccgct gccaacagca acaattacag
      421 cattagggcc tttaagcttt tgcaatggcg atagtgtaaa acttgtcgct aacagtttgg
      481 taggcgccac atataagtgg aaaaagtatt ccaattttat agcaaatgct acatcaataa
      541 attataccgc aaaaacaagc ggtgtatata aagcgcagat aactgatatt aatggttgca
      601 gcaaagtatc taatacttta acggtgaatg tgaattgcag ggagcaaaat gatttggcag
      661 cccaaagtaa catacttatt gaaccaaatc cgtttaatga aaacacaact attacatggc
      721 ccggttattt tggaaaaaac attgacatta aaatttataa tactaccgga agtttaatga
      781 aagatgaaag cattaagcaa actgataaca gtgctaaaat aactataaag ccagcaggac
      841 tatattttgt tgtgattaca actgccaatg aggccatagt aaaacgtatc atgaaagtag
      901 attaacattg tttccttttg ctgagtgtag ctttttggtt tcacttaaat tcagcaatca
      961 tatggatagg aattagtttg ttttgatttt tcggaagcga ataaaaaaag ccatctcatc
     1021 ttgaaatggc ttttgtgatt tctacatctt ttatttttta ccggatgctt tttgtccttt
     1081 atggtgcttt ttgagtttta ctttttttaa tgctttttgc gcatctgttt ttactttttt
     1141 aactttagct tctgctgctt ttacagtagg tttagctgcg tcttgtgcca ccgcttttgc
     1201 ctgctctgct ttttttgttg cggtagcctg tgccgctttt acaacaggtt ttgtttgatc
     1261 ttgtgcaaat gcaacacctg ccatcaagaa ggatataatt gcaactagaa actttttttt
     1321 catatctgtt tgttttaaat tgttggagcg aatttgggtt taaatttatg aatcgcgtat
     1381 gaatgtgctt ttatagctat tttgagtgca cgttttttag caattattta tcatcgggta
     1441 atcaaattgt ttttgaaata tataatcact ataacgataa caatatactt tagtgaaaat
     1501 tttgcagcct taaatttcag aaaaccgaat agagtaaaat gatccaaatt gtacctacaa
     1561 attatgttaa cattcctcca tctacattta taacctggcc ggtaatatat gtcgaattat
     1621 cgctggcaag aaaaacaaca aggttggcta catcttgtgg agtgccacca cgctttagcg
     1681 gtatagcttc acgccattgt tttactgttt cctgattgag tgctgctgtc atttcggttt
     1741 caataaaacc cggtgctatg gcattgcatc gtatgttgcg cgaaccaagc tcgagtgcaa
     1801 tactttttgt aaagcctata atgccagcct tacttgcagc ataatttgcc tggccagcat
     1861 tgcctttaac gcctaccaca ctacttatat ttattatgct tccactacgt tgttttagca
     1921 gttgcctttg aacggctttg gtaatattga atacactttt gagattcgta ttgatcactt
     1981 cgtcaaaatg ttgttcgctc attcgcatta gcaagttgtc gcgtgttatg cccgcattgt
     2041 ttacaacaat atctatagtt ggaaattgtg caagtactcc ggccaccaga ttttcggcag
     2101 cggtgtaatc ggcagcattg gactggaatc caattcctgt aacaccaaag tgttttattt
     2161 cgtcctgcaa caacatggct tgctcggttg acgagttgta ggtaaaagca atatttgctc
     2221 cctgctcggc caatgccagt gctatgccac gcccaatgcc gcgcgatgct ccggtaataa
     2281 gtgccacttt gcctgctaat aatttcatat gtatgtttta ttttaaggtt aaaaaaaatt
     2341 atatttgcca aatatattta atctaaaata aaaataaaat atgaaaagaa ttattatttg
     2401 tgtaatggta ctgatggcaa cgcaacttgt gcaaagccaa tcgatgatga taggtgtaaa
     2461 aggtgcatac aatacaacat
//
LOCUS       DAORQA010000260         2471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9676_length_2471_cov_8.557119, whole genome shotgun sequence.
ACCESSION   DAORQA010000260 DAORQA010000000
VERSION     DAORQA010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9676_length_2471_cov_8.557119"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PK736_08800"
     CDS             <1..843
                     /locus_tag="PK736_08800"
                     /inference="COORDINATES: protein motif:HMM:NF040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5913 domain-containing protein"
                     /protein_id="HRC33525.1"
                     /translation="EGWLDFVITAKAKSKIKQTIKEEFRLKSDQGREIFERKLKRLKL
                     NKGHFNINYLLEYFKIKSANDLYFRIANESLDFAEIKDYLLNKTQIDAQLQLKQKEAM
                     TFEDLLRAKRGNDDMLVIGDNLDKIDYKLSVCCNPIPGDDVFGFITINEGIKIHRTTC
                     TNAIQLMSNYAYRIVKARWNNQKELAFLVGIKIKGVDDVGVVNNITKVISSELKVNMR
                     SISIDGAEGLFNGTIMLFVHDTVQLNKLMSKLSQISGILSVIRFDMENELEFGRKMPN
                     KDLN"
     gene            1589..>2471
                     /locus_tag="PK736_08805"
     CDS             1589..>2471
                     /locus_tag="PK736_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33526.1"
                     /translation="MKNYKKTTVLIAYAIIILNSQKINAQWNYDLSINNPISLAANDQ
                     QDARLVSNGDKGAIIAWVDYRSNVTVADIFVQRIDKNGYIKWAVNGLPICTSNNDQKE
                     VNITEADNGSAIVVWSDSRNGFEKDVYAQKIDSNGTILWTANGVSLSSLASTKQNPKV
                     LHDGNGGAIVTWQDSSVAANDFDIKAQHVDMNGVITWSTTGENICAFTNKQINPRLET
                     DNANGAIITWQDKRFNNFYDIYVQHITAAGTVAWTANGFIICNANEGQLNPKIEPDGS
                     GGAIIAWQDKRNNNYDVY"
BASE COUNT          888 a          399 c          483 g          701 t
ORIGIN      
        1 gaaggatggc tcgattttgt tattactgca aaagcaaaaa gtaaaataaa gcaaaccatt
       61 aaagaagagt ttcgtttgaa aagcgatcag ggtcgcgaaa tttttgaacg caaattgaaa
      121 cgacttaaac tgaataaagg ccattttaat ataaactatt tgctcgaata ttttaaaata
      181 aaaagcgcca acgatttata cttccgcatt gcgaatgagt cgctcgactt tgccgagata
      241 aaagactacc tgttaaacaa aactcagata gatgcgcaac tgcaactcaa gcaaaaagag
      301 gctatgacgt ttgaagattt gttgcgtgca aaacgtggta acgatgacat gttggtaata
      361 ggcgacaatc tcgataaaat agattacaaa ctttcggtgt gttgcaatcc tataccgggc
      421 gatgacgtgt ttggttttat taccattaac gaaggaataa aaattcaccg caccacctgc
      481 accaatgcca tacagctgat gagtaattat gcttaccgca tagtaaaagc aagatggaac
      541 aatcaaaaag agcttgcgtt tttagttgga ataaaaataa aaggtgtaga tgatgtaggc
      601 gttgtaaata atattacaaa agtaatatcg agcgaattga aagtgaatat gcgcagtata
      661 agtattgatg gtgcagaagg attattcaat ggcaccatta tgttgtttgt gcacgataca
      721 gtgcagttga ataagttgat gagcaagctt agtcagatat cgggcatact cagtgttata
      781 cgtttcgaca tggaaaatga acttgagttt ggccgtaaaa tgccgaacaa agatctgaat
      841 tgaggaagtt gatttaacag ctttatatgc catattggta aggtgataat ctgaagcatc
      901 tcttaccacc tcatgaagtt gctgtttgcc aataaacaaa tcaaggtaaa gtatacatct
      961 taaaatatgg aactaaaata cgttatctta aatgcaatca ttttaatttt tgtaacgcaa
     1021 tatttttttc atactgtaaa gttcgccctt gcttttaaca agaatatttt tttcgtgggt
     1081 tggaagaaaa ttttccattc agtgatgata tggttagtgc cttatttatg aatattttta
     1141 ctcaaaggat tcatgactcc attacccggc actgctactt cgcgaaaaaa tttctacaat
     1201 tcaaaaataa aaaatacagg agtgggttta tgagttcttt ttggactaag caaccatgaa
     1261 gctaataata ctgaccaaca tgttggcgat aatgctaaaa actacaatga tggttatttt
     1321 gatagtaatg atgctggtga ttatggcaga agcgatagca gttctgctga tgctgacatc
     1381 acttgaacta actaaattta aggccttact ttttacttca aaaaaaaaat tcgtttataa
     1441 aacttttacc aaataaaagt attcaaacaa ttctactatt ttatcaaagg cttactagaa
     1501 atgttgtaaa taatttgaac attaatagga aaatttaata catattcgca gcctgaaaca
     1561 aacttaacaa tttgataata tagtaaaaat gaaaaactat aaaaagacaa ccgtgcttat
     1621 tgcgtatgca attataatat taaatagcca aaaaataaat gcacagtgga attacgattt
     1681 aagcataaac aatcctattt cgttagccgc taacgatcaa caggatgccc gcctggtaag
     1741 caatggcgat aaaggtgcaa tcattgcctg ggtcgattac agatcgaatg ttactgtagc
     1801 agatattttt gtgcaacgaa tagataaaaa cggatacata aaatgggcag tgaatggttt
     1861 gccgatatgc acatcaaata acgatcaaaa agaagtgaat attaccgaag ccgataatgg
     1921 ttcggcaata gtggtgtggt ccgattcgcg caatggattt gaaaaagatg tttatgccca
     1981 aaaaatagat tcgaatggaa caatactgtg gaccgctaat ggtgtttcgc tttcgtcact
     2041 ggcatccaca aaacaaaatc ccaaagtgtt gcacgatggt aatggcggtg caatagttac
     2101 ctggcaagat agcagcgttg ccgcaaacga cttcgatatt aaagcccagc atgttgatat
     2161 gaacggagta ataacatgga gtacaacagg agaaaatatt tgcgctttta caaataaaca
     2221 aattaatcca cggctcgaaa ctgacaatgc taatggtgca ataattacct ggcaggataa
     2281 acgcttcaat aatttttatg atatatatgt acaacatatc accgctgcag gtacagttgc
     2341 atggacagca aatggattta tcatttgcaa tgcaaacgaa ggtcagctaa atccaaaaat
     2401 agaacccgat ggcagtggtg gggcaataat tgcctggcaa gataaacgca acaataatta
     2461 cgatgtgtat g
//
LOCUS       DAORQA010000261         2449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9774_length_2449_cov_6.073935, whole genome shotgun sequence.
ACCESSION   DAORQA010000261 DAORQA010000000
VERSION     DAORQA010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9774_length_2449_cov_6.073935"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..2338
                     /locus_tag="PK736_08810"
     CDS             113..2338
                     /locus_tag="PK736_08810"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRC33527.1"
                     /translation="MSFKSACVIAILFMIALTNAQAQLISIANARALPVGSVVNVEGV
                     CLNGFELGSIRYMQDATGGIAVFSTLLSTTTTGDEVNVTGVTVQYSNLLEIQPVNSWA
                     LNSSGNALPAPALITPVQMDEPYESQLVRIDDATFANGGSLFQANTNYQINANGQTGV
                     MYVRTGSALVGTLIPFTSVDLIGICSQYQTQYQLLLRSPADMISNSAVSILNTPYQTN
                     IVTTGFDVSWQTNVNSTSQLLYGKTPALELGILTGTGNVTSHTVTVTGVNPSDVIYVQ
                     AISVLGTDTAFSEKRIFITASGSTGEIKTYFNRTVDINFANPANNIATQLNYTIDDTL
                     KAYMDRAVSTIDIAIYSFDDFNIAKITQGINDAYNRGVQIRIIADGQNVNAGLSTLNA
                     NIPILHSPDTPANYYSIMHNKFVIIDAASSDVNLPIVWTGSTNWSDNQLYTDANNVII
                     IQDKSLAKAYTIEFEEMWGSSGAQYNLNNIKFGPKKTNNTPHEFLINGKRIENYFSPS
                     DNVNAQIERTIQTANSSLQFATFVFTRTDLAYAVQDRVWNNVDANGMVDDSSSGGGFA
                     FSIMENVMNNKIILYDHNSLQGILHHKYLIVDQDNPASDALVLTGSHNWSTTANTKND
                     ENTLMVHDASIANQYYQEFVKRFNESGGGLYFYENSESSPVSFYPNPTTENLYFNWEA
                     TSWDDVTITIYDVHGKVVFTKNDSVKQFVNINVSSFMPGLYQLVLQNDKRRICTKFVK
                     Q"
BASE COUNT          770 a          455 c          498 g          726 t
ORIGIN      
        1 aatacagacc atgcatttcg aaagggatgc atggtttttt tttactttaa taatttgaat
       61 catgtgtaat caaaataaaa tttatacgac aactcttaga atacatcact gtgtgagctt
      121 caaaagtgca tgtgttattg caatattgtt tatgatagca ctaaccaatg cccaagcgca
      181 attaataagc attgctaacg cacgggcatt gcctgttggc agtgtggtaa atgttgaagg
      241 tgtttgcctc aacggattcg aattaggaag cattcgatac atgcaagatg caactggtgg
      301 catagcagtt tttagcacgt tactatctac caccaccacc ggtgacgaag tgaatgtgac
      361 tggtgtaact gttcagtatt caaacttgct cgaaatacaa cctgtcaatt cttgggcact
      421 caattcatcg ggaaatgcac ttcccgctcc agcacttatt actcctgtgc aaatggacga
      481 accttatgag tcgcagttgg tacgaattga cgatgctaca tttgcaaatg gcggatcatt
      541 gtttcaggca aacacaaatt atcagattaa tgccaacgga caaacgggcg taatgtatgt
      601 ccgcacgggg agtgcactgg taggaacgct gattcctttt acgagtgttg atttgattgg
      661 tatatgttct caatatcaaa cccaatacca attgcttttg cgcagcccgg ccgatatgat
      721 ttcaaattcg gcagtaagta ttttaaacac tccataccaa accaatattg ttaccacagg
      781 cttcgatgta agctggcaaa caaatgtaaa ttcaacctcg cagttattgt atggaaaaac
      841 acctgcactc gaattaggaa ttttaaccgg cacaggcaat gttacttcgc atacggttac
      901 cgttacagga gtaaatcctt ccgatgtgat ttatgtacaa gctatttcgg ttttgggcac
      961 cgatactgca tttagtgaga agagaatatt tattactgct tcgggctcta cgggcgaaat
     1021 taaaacttac tttaaccgta ccgttgatat taactttgct aaccctgcaa ataatattgc
     1081 cacacaactt aactacacaa ttgacgatac tttgaaagct tacatggacc gtgcggtttc
     1141 cactatcgat attgccatct acagtttcga tgattttaat attgctaaaa ttactcaagg
     1201 cattaatgat gcatacaacc gtggtgtaca gattcgaatt attgccgatg gacaaaatgt
     1261 aaatgccgga ttaagtacac tcaatgccaa tattccaatt ttacattcgc ccgatactcc
     1321 tgcaaattac tatagcataa tgcataataa atttgtgatt attgatgcgg catcttccga
     1381 tgttaatctc cctatagtgt ggacgggttc tacaaactgg agcgacaatc aattgtacac
     1441 cgatgcaaac aatgtaatta tcattcaaga caagtcgctg gcaaaggctt acaccattga
     1501 atttgaagag atgtggggca gcagtggcgc acagtacaat ttaaataata ttaagtttgg
     1561 gccaaagaaa acgaataaca ctccccacga atttcttatc aatggcaagc gtatagaaaa
     1621 ttatttttct ccatcagata atgtgaatgc tcagatagaa cgtaccattc aaacggctaa
     1681 cagttcattg caatttgcaa cttttgtttt tacgcgcacc gacttagctt atgccgttca
     1741 ggatcgcgtt tggaataatg ttgatgcaaa tgggatggtg gatgactctt caagtggagg
     1801 gggttttgca tttagtataa tggaaaacgt gatgaacaat aaaattatat tgtatgatca
     1861 taatagcttg cagggaatat tacaccataa atacctgatt gtagatcagg ataatcccgc
     1921 ctcggatgca ttggtgctta ccggctcgca taactggagt acaactgcaa atacgaagaa
     1981 tgatgaaaat acattgatgg tgcacgatgc atcaattgcc aatcaatact atcaggagtt
     2041 tgtaaaacga tttaacgaga gtgggggagg attatatttt tatgaaaata gtgagagttc
     2101 acctgttagt ttttatccta atcccactac agaaaattta tatttcaatt gggaagcaac
     2161 atcttgggat gatgtaacaa taacaatata cgatgtacac ggtaaagtgg tatttactaa
     2221 gaacgattca gtaaagcagt ttgtaaatat taatgttagc agtttcatgc cgggtttgta
     2281 tcaattggtt ttgcaaaacg ataaaagaag gatttgtact aaattcgtga aacagtaact
     2341 tgtatttttt gctgcacctc cattgccaat ggagattttt aaatggatgc aaaagctggc
     2401 aaaggcttac accattgaat ttgaagagat gtggggcagc agtggcgca
//
LOCUS       DAORQA010000262         2448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9775_length_2448_cov_11.218554, whole genome shotgun sequence.
ACCESSION   DAORQA010000262 DAORQA010000000
VERSION     DAORQA010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9775_length_2448_cov_11.218554"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PK736_08815"
     CDS             complement(<1..753)
                     /locus_tag="PK736_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33528.1"
                     /translation="MKSINSVVKSKYFQSANVLSLHRFNILLAFACMVFCTDAKHKSL
                     GGSSRLLLEDDKYSYANFKMLFTKVDLPFCFDTDSLRDDFVYSRDGELYDSIGNHKSA
                     SLIPKEFVLKWMIDTTGKHKATLLDTFINKQNFESYFNDKFYWNTIEPFIMISKGVDD
                     VFFVLIYNQTSAMNVGYTEVLALTYTQDGLLKKVKSIGRYGVYIRTETKDRLHIFRWT
                     KFIDAVSLQIQIKTANKVIVTIINKTEKEVQEI"
     gene            complement(761..>1817)
                     /locus_tag="PK736_08820"
     CDS             complement(761..>1817)
                     /locus_tag="PK736_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33529.1"
                     /translation="KIFTGMLPTEMNVANTSVTWNGRDWAMIMLPLPSNYHDRINLFA
                     HELFHVAQPSLQLKFMNVDNNHLDKKAGRVYLRLELEALIKTVQATNERDINNHLTNA
                     LSFRMYRQSLFSGSDTTENLLELNEGLAEYTGTVISNRTTAVAKQHFENDISGFFSNP
                     TFVRSFAYHTTPVYGYLLSKYKGNWIKQISNRTNLTAFFINEFGISLSGDLMKFVSDI
                     SEDYNGTKIISEENEREEKTNKLIADYIDKFVTQPHFEITFEQMQVSFDPGNIMPLNE
                     HGTVYPNMRIVDKWGILTVSNGALMVPNWDRIVVTIPKNSGAKKLNGDGWTLEINDGY
                     SVIKNSNTGNYELEKIK"
     assembly_gap    1818..1841
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1842..2064)
                     /locus_tag="PK736_08825"
     CDS             complement(<1842..2064)
                     /locus_tag="PK736_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33530.1"
                     /translation="MKKITTSFLLLFYTIITSAQQKKLSPDSAAEYFEDIKTETKKHQ
                     NLWNKDLYGPILLVDPQTRELIANFADTAG"
     gene            complement(2238..>2448)
                     /locus_tag="PK736_08830"
     CDS             complement(2238..>2448)
                     /locus_tag="PK736_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33531.1"
                     /translation="YLPFADEISCEAPQPTKTANTKTFASQNGKSKINARYIKVVANN
                     LKTCPRWHIGAGYNCWIFCDEIVVR"
BASE COUNT          746 a          469 c          392 g          817 t
ORIGIN      
        1 aatttcttgc acttctttct ctgtcttgtt aatgattgtt acaattacct tgttggcagt
       61 tttaatttgt atttgtaacg atacagcatc aataaacttt gtccaacgaa aaatgtgcaa
      121 cctatcttta gtttcggtac gtatatatac accgtatcgg ccaatagatt ttactttttt
      181 caacaaacca tcttgtgtat aggtcaatgc cagcacttcg gtatagccaa cgttcattgc
      241 acttgtttgg ttataaatca atacaaaaaa tacatcgtct actcctttgc taatcataat
      301 gaaaggctca atggtattcc aataaaactt atcgttaaaa taactttcga aattttgctt
      361 attaataaaa gtatcaagta aagtggcctt atgcttgcca gttgtatcta tcatccattt
      421 taaaacaaat tcttttggta ttaagcttgc acttttatgg ttgccaatac tatcgtacaa
      481 ttctccatca cgtgaataaa caaaatcatc cctcaacgaa tctgtatcga aacaaaatgg
      541 aagatctact tttgtaaaaa gcatcttgaa attagcatag gaatatttat cgtcctctaa
      601 aagtaaacgc gaactaccgc ctaagctttt gtgctttgca tctgtacaaa ataccatgca
      661 tgcgaacgca agaagtatgt tgaatcgatg cagcgataat acatttgcac tttgaaaata
      721 cttagacttc acaacagagt ttattgattt caactggtag ctacttaatt ttttctagtt
      781 cataatttcc agtatttgag tttttgataa cactgtatcc atcattaatt tccagtgtcc
      841 atccatcacc attcaatttt ttcgcaccac tatttttagg aattgtgaca actattctat
      901 cccagttagg caccattagc gcgccattcg aaaccgttag tattccccac ttatcaacta
      961 tccgcatatt agggtaaacg gtaccatgtt cattcaaagg cataatattt cccgggtcga
     1021 aagaaacttg catttgctca aatgtaattt cgaaatgagg ctgtgttaca aatttatcaa
     1081 tatagtccgc aataagttta ttcgtttttt cctcacgctc attttcttct gaaataattt
     1141 ttgtgccatt gtaatcttct gaaatatcgc taacaaactt catcaaatca ccggacaaag
     1201 aaattccaaa ttcgttaata aaaaaggcag taagattagt gcgatttgaa atttgcttga
     1261 tccagttgcc tttatatttc gaaagtaaat agccataaac cggagtggta tgatatgcaa
     1321 acgagcgcac aaaagttgga ttactaaaga acccgcttat atcattttcg aaatgttgct
     1381 ttgccactgc cgtagttctg ttactgataa ctgtgcctgt atactctgca agtccctcgt
     1441 taagttcaag caaattttcg gttgtatcgg agccggaaaa taatgattga cggtacattc
     1501 gaaatgacaa tgcattggtt aagtgattat ttatatccct ttcatttgtt gcctgtactg
     1561 tcttaatcaa tgcttctaac tccagacgca aataaactct gccagccttt ttgtcgaggt
     1621 gattattatc gacattcata aatttcaact gcaatgaagg ctgtgcaaca tgaaataact
     1681 cgtgcgcaaa caaattaatt ctatcgtggt aattagaagg caaaggcaac attatcattg
     1741 cccaatctcg gccattccat gtaacgctgg tattggcaac attcatttca gttggcaaca
     1801 tcccagtaaa tattttcnnn nnnnnnnnnn nnnnnnnnnn ncacctgctg tatcagcaaa
     1861 gtttgcaatc aactcacgag tttgcggatc caccagcagt attggcccat ataaatcttt
     1921 gttccataaa ttctggtgct tttttgtttc cgtttttata tcttcgaaat actctgcagc
     1981 actatcgggc gatagttttt tctgttgcgc agaagtgatg attgtgtaaa ataacaataa
     2041 aaatgaggtg gtgatttttt tcatttttta attcaacgtt aaattaaaca tggagaattc
     2101 aaattggtta ccgcactttt ctatcaaaaa agtaaccttt caaaagtaac aaagaccgat
     2161 cgaaaatcgg gatctacaaa agttgttttt tcgttaaaac ttataattga aaactaaagt
     2221 gatgaatgca acacaattta tcgcacaact atttcatcgc aaaaaatcca gcaattgtat
     2281 ccggcaccaa tgtgccagcg aggacacgtt ttcaaattat ttgccactac tttaatgtat
     2341 cgtgcgttga ttttggactt gccattttgc gaagcgaatg ttttagtatt cgcagtttta
     2401 gttggttgtg gtgcttcgca acttatctca tcggcaaagg gcaaataa
//
LOCUS       DAORQA010000263         2439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9811_length_2439_cov_5.429950, whole genome shotgun sequence.
ACCESSION   DAORQA010000263 DAORQA010000000
VERSION     DAORQA010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9811_length_2439_cov_5.429950"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /gene="fusA"
                     /locus_tag="PK736_08835"
     CDS             <1..872
                     /gene="fusA"
                     /locus_tag="PK736_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780772.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRC33532.1"
                     /translation="QTDMDKLGMALNKLAEEDPTFKVHTDHETGQTVISGMGELHLEI
                     IVDRLKREFKVECNQGAPQVAYKEALTGSSTHREVYKKQTGGRGKFADIQFEISPADQ
                     EWQEKGNTGLQFVNEVTGGNIPREFIPAVQKGFDASMINGGLAGYQLESMKIRLFDGS
                     YHAVDSDALSFEICAKVAFREVYTKARPVLMEPIMKVEVVTPEENMGDVIGDLNKRRG
                     QLEGMDSRAGAQVIRAKVPLSEMFGYVTSLRTLSSGRASSTMEFSHYAEAPRNVAEEV
                     MAKAKGKKLASQD"
     gene            913..1218
                     /gene="rpsJ"
                     /locus_tag="PK736_08840"
     CDS             913..1218
                     /gene="rpsJ"
                     /locus_tag="PK736_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRC33533.1"
                     /translation="MSQRIRIKLKSYDYNLVDKSAEKIVKTVKSTGAMVSGPIPLPTE
                     KQTYTVLRSPHVNKKAREQFQLCSYKRLLDIHSSTAKTVDALMKLELPSGVEVEIKV"
     gene            1303..1920
                     /gene="rplC"
                     /locus_tag="PK736_08845"
     CDS             1303..1920
                     /gene="rplC"
                     /locus_tag="PK736_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRC33534.1"
                     /translation="MSGIIGKKIGMTSMFDSEGKNIPCTVIEAGPCVVTHVKTKEKDG
                     YSAIQLAFSEKKEKSTTMAMKGHFKKAGTTPKRKLVEFLGFENPLNLGDIIKCDLFAE
                     GEFCDVVGTSKGKGFQGVVKRHNFGGVGGQTHGQHNRLRAPGSLGASSWPSRVMPGMR
                     MAGHMGHARIKVQNLKIVKVLADQNLILIKGAVPGHNGSFVLLEK"
     gene            1924..>2439
                     /gene="rplD"
                     /locus_tag="PK736_08850"
     CDS             1924..>2439
                     /gene="rplD"
                     /locus_tag="PK736_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRC33535.1"
                     /translation="MQVSVINTSGSETGRTVTLNESIFNAEPNQHAIWLDVKQFLANQ
                     RQGTHKTKERAEIARTTKKLKRQKGTGGARAGSMKSPLFRGGGRVFGPQPRDYSFKLN
                     KKLKRLARVSALSSKAKNGGILVLEDFNYSAPKTKDFAGMLNKLSVTEKKSLLVLSET
                     NKNIYLSSRNLQ"
BASE COUNT          831 a          406 c          539 g          663 t
ORIGIN      
        1 ctcaaaccga tatggacaaa ttgggaatgg ctcttaataa attggccgaa gaagatccta
       61 cgttcaaagt gcataccgat catgaaactg gacaaactgt tatcagtgga atgggtgagt
      121 tacatttgga gattattgtt gaccgtctta agcgcgaatt taaagttgag tgtaatcaag
      181 gtgcacctca ggtggcatac aaagaggcat taacaggaag ttctactcat cgtgaagtat
      241 acaaaaagca aaccggtggt cgtggtaagt ttgccgatat tcaatttgaa ataagtcctg
      301 ccgatcaaga atggcaagaa aaaggtaata caggattaca gttcgtaaac gaagttaccg
      361 gtggaaatat tcctcgtgaa tttattcctg ctgtacaaaa gggattcgat gcatctatga
      421 taaatggtgg tttggcaggt tatcaattag aaagcatgaa gattagattg tttgatggtt
      481 cttaccatgc agttgactcg gatgcattat cctttgaaat atgtgccaaa gtggccttcc
      541 gtgaggttta tactaaagct cgcccagttt tgatggagcc aattatgaaa gttgaagttg
      601 ttacacctga agaaaatatg ggggatgtaa taggtgactt gaacaaacgc agaggccaac
      661 tagaaggaat ggattcgcgt gcaggggctc aagttataag agcaaaagtg cctttatcgg
      721 aaatgtttgg ttacgtaaca tcattgcgta cattatcatc gggccgtgca tcatctacaa
      781 tggagttttc acactatgcc gaggcaccac gtaatgttgc tgaagaggta atggccaaag
      841 caaaaggtaa gaaattagca agtcaagatt aagttttgtt caaattaaat aacgttcttt
      901 aataataaat caatgagtca gcgtatacgc atcaaactaa aatcttacga ttataattta
      961 gttgataaat cagcagagaa aattgtaaaa actgtaaaga gtacaggcgc tatggtaagt
     1021 ggaccaattc cattaccaac cgaaaagcaa acatacactg ttttgcgttc gccacacgtt
     1081 aacaaaaaag ccagagagca atttcaatta tgctcctaca agcgtttgct tgatatacat
     1141 agctcaactg caaaaactgt agatgcattg atgaaacttg aattgccaag cggtgttgaa
     1201 gtagaaatta aagtctaagt tttaagaaat attagatttt aagcatataa atacgttctt
     1261 aaataaagtt ggtaggaata tcaacctctt ataaaaaata ttatgtcagg aattatagga
     1321 aaaaaaatcg gaatgacgag tatgttcgat tccgaaggga aaaatatccc ttgcacggta
     1381 atagaagccg ggccatgtgt agtaacacat gtaaaaacaa aagaaaaaga tggctattca
     1441 gctattcaat tggccttctc tgagaaaaag gagaagagta caactatggc aatgaaaggt
     1501 catttcaaga aagcaggtac tactcccaaa cgtaaacttg ttgagttttt gggatttgaa
     1561 aatcctctca acttaggtga tataatcaaa tgcgatttat ttgctgaggg cgaattttgc
     1621 gatgtcgttg gcacctccaa aggaaaagga tttcagggtg tggtaaaacg ccacaatttt
     1681 ggaggagtag gtggtcaaac tcacggtcag cataatcgac ttagagctcc cggttctttg
     1741 ggtgcttcgt catggccttc gagggtaatg ccaggaatgc gtatggccgg ccatatgggt
     1801 catgcccgta taaaagtgca aaacttgaaa atagtaaagg ttttagccga tcagaattta
     1861 atattaatta aaggagctgt tcccggacat aacggtagct tcgtattatt agaaaaataa
     1921 atcatgcaag tatcagttat aaatacctcc ggaagcgaaa cagggcgcac agtaacccta
     1981 aatgaaagca tttttaatgc tgagcctaat caacatgcga tttggttgga tgttaagcag
     2041 ttccttgcaa atcaacgtca gggaactcat aagacaaagg aacgtgcaga aattgcacgt
     2101 actacaaaaa aattgaaacg tcaaaaagga accggtggag ctcgtgcagg aagtatgaag
     2161 tctcctttgt ttagaggagg aggtcgtgtt tttggtcctc agccacgtga ctatagcttt
     2221 aaattgaata aaaagttaaa gcgtttagcc cgtgtttcgg cactatcatc taaagctaaa
     2281 aatggtggca tattagtgct tgaagatttt aattattcgg cacccaaaac caaagatttt
     2341 gcaggcatgc taaataaact ttcggttaca gaaaaaaaat ctttgttagt gttgtcagaa
     2401 accaacaaaa acatatattt gtcctcccga aatttgcag
//
LOCUS       DAORQA010000264         2436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9823_length_2436_cov_7.679966, whole genome shotgun sequence.
ACCESSION   DAORQA010000264 DAORQA010000000
VERSION     DAORQA010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9823_length_2436_cov_7.679966"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..1056)
                     /locus_tag="PK736_08855"
     CDS             complement(247..1056)
                     /locus_tag="PK736_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC33536.1"
                     /translation="MKPITTIIVDDQQANINTLQTDLYEHADKIEILATFTEPTKALI
                     AIKELKPDVLFLDIEMPVIDGITLARQTSGYYHEVVFVTNFNKYMRDAFKVHAFEFLI
                     KPIKDADLAEVIESLATKALKGWNSKSEERIDALARNNKENRPIPDALYCQRFSRDNW
                     DLIKWSDIVMFEMDTNSELGIFTNNGQHYFRNTTLALIEKFIDEQPENIATQFIKINR
                     VHIVNYNYIKAFNPVDSMLKLKDEIKIHDLKPLKVNDNVKAMFRKMMIPAR"
     gene            complement(1053..>2436)
                     /locus_tag="PK736_08860"
     CDS             complement(1053..>2436)
                     /locus_tag="PK736_08860"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HRC33537.1"
                     /translation="IHTNHHFHLQTEVESLQSILNAINYNRELKYFNLIFVGALSMFA
                     LFFFCQYLQQRSEHTFLIYVCYVTCTVLHALREFVRFQGLPGLHATFTNWYYFSEIPL
                     AYSIYTLYTLFFIFLLDLKRLNGFAFKYARFVVVLFMLNLLLDIYLKIFHSIHLSYTF
                     FYFLRTPLLILTLPILIFSYKSRSTSAKYFFYGSLALLIGVLFNLAMSTLQRFGQFEY
                     LFNTSKESIFWKSYIVYSRIGMLIEVVFFSLGLAHKSKLEYSTASNDALKSNFASMIN
                     HSVSNALNKLKGMIKRKEEGVIPFIDETGAMMQNMLSVVRNQISLSQELILAQQFFDF
                     RNENSQAINFKINRSNCNTDNYFVPPMLLQPFIENCVEHAWSEDDIEKKIQINIVVQN
                     KFIAIEIIDNGNGIVENNLKEKSHGMFLIAERINYFNTIFGCNISCQIKNNSSTKGTT
                     VTIKNIPLKK"
BASE COUNT          784 a          396 c          408 g          848 t
ORIGIN      
        1 tatgttaaac ttgttacttt atcatttata tccaaaactc tttaacatta ttatagaata
       61 gttgttgaca aatttatagc attatcaaat aacataacaa cattttgggt ttagatgcaa
      121 cgaacctcaa aaaaggtgca gcgaaccact tactctgtaa tggagttttg gagttgattt
      181 agaaaacaag acgttaaatg gcttgtagat tttgtaatat acggatgtaa ataaccgttt
      241 gcaaaatcac cttgctggaa tcatcatttt tcgaaacatg gcttttacat tatcgtttac
      301 ttttaatggt ttgaggtcgt gtatttttat ttcatctttt agtttcagca tgctatcaac
      361 agggttaaaa gcttttatgt aattgtaatt tactatgtgc acacggttta ttttaatgaa
      421 ttgtgtggca atgttttcgg gttgttcatc aataaatttt tcaatcaagg caagtgtagt
      481 attacgaaaa taatgttggc cattgtttgt aaaaatgccc agttcgctat tggtatccat
      541 ttcaaacatt acaatatcgc tccattttat caagtcccag ttatcgcgcg aaaatcgttg
      601 gcaatacaag gcatcgggta tgggtctgtt ctctttgttg ttgcgtgcta atgcatctat
      661 tcgttcttcg cttttgctat tccaaccctt aagcgcctta gtggcaaggc tttctatcac
      721 ttcggctagg tcggcatctt ttatgggttt tatcaaaaac tcaaatgcat gcaccttaaa
      781 agcatcgcgc atgtacttat taaaatttgt tacaaacacc acctcgtggt aatatcccga
      841 tgtttgcctg gcaagggtaa tgccatctat caccggcatt tctatatcaa gaaaaagcac
      901 atctggtttc aattctttta tagcaattag tgcttttgta ggttctgtaa aagttgctaa
      961 aatttcaatc ttatccgcat gttcataaag gtcggtttgt aacgtgttta tgtttgcttg
     1021 ttggtcgtca acaataattg ttgttatagg cttcattttt ttagtggtat attttttatg
     1081 gttacagtag ttcctttggt gctcgaattg ttttttattt ggcaagaaat attgcaccca
     1141 aaaatagtat taaaataatt aatacgctcg gcaattaaga acataccgtg tgatttttct
     1201 tttaaattat tttctacaat gccattacca ttatctataa tttcgattgc gataaattta
     1261 ttttgcacaa cgatatttat ttgtattttc ttttcaatat cgtcttcgct ccaggcatgt
     1321 tctacacaat tctcgataaa aggttgcagc aacattggag gcacaaaata attatcggtg
     1381 ttgcagttgc ttctattaat tttaaaattt attgcttgcg aattttcgtt tctaaaatcg
     1441 aaaaactgtt gtgccaaaat taattcctgt gaaagcgata tttgattacg caccacactt
     1501 aacatatttt gcatcattgc cccggtttca tctataaagg gtattacacc ttcttccttt
     1561 cgttttatca ttcctttcaa tttgttgagg gcgttactaa cagagtgatt tatcatggat
     1621 gcaaaattac tttttagtgc atcgttgcta gctgtggagt attctaattt agatttgtgc
     1681 gctagtccaa gactaaaaaa aacaacttct attaacattc caatgcggct gtatacgata
     1741 tagcttttcc aaaaaatact ttctttactc gtgttgaata agtattcaaa ttgtccaaat
     1801 cgttgcaaag tgctcattgc taaattaaag agtacgccaa ttagcaatgc aagcgaacca
     1861 taaaaaaaat actttgcaga agtactacgt gacttgtaag aaaagattag aattggcaaa
     1921 gtgagaatca aaagtggtgt acgcaaaaag taaaagaaag tataactcaa atgtatggaa
     1981 tgaaatattt ttaaataaat atctaatagt aaatttaaca taaataacac cacaacaaaa
     2041 cgggcatatt taaatgcaaa tccatttagt ctttttaaat cgagaagaaa aataaaaaac
     2101 aaggtatata gagtatagat gctgtaagca agtggtattt cactaaaata ataccaattg
     2161 gtaaatgtgg catgtaaacc aggcaaacct tgaaagcgaa caaactcacg caaagcatgc
     2221 aagactgtgc aagttacata acatacatag atgagaaaag tatgttctga cctttgttgc
     2281 aaatattggc aaaagaaaaa tagagcaaac attgacaacg caccaacaaa tattagatta
     2341 aaatatttca attccctatt atagtttatt gcgttgagga tactttgcaa actttcaacc
     2401 tcggtttgca aatggaaatg atgattagta tggatt
//
LOCUS       DAORQA010000265         2431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9859_length_2431_cov_5.497896, whole genome shotgun sequence.
ACCESSION   DAORQA010000265 DAORQA010000000
VERSION     DAORQA010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9859_length_2431_cov_5.497896"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..>2431)
                     /locus_tag="PK736_08865"
     CDS             complement(310..>2431)
                     /locus_tag="PK736_08865"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRC33538.1"
                     /translation="NTSFNDVFAFFLSGPGIVGLKNIALIPNTTLPVSINNINNGNAN
                     AGPCMNCTYFYDNFGGTTVQYDAFTTVLTAQSAVIACDTFHIKLAIADVADGVLDSGV
                     LLEAGSFTGGIPNVLAQFSSAYAANDSSIIEGCTDVTVTFSYPNPVPNDVYIKLNISG
                     TAVNGVDYNQLPDSVKIAAGDSSGFISIYPTPDGLTEGTETIIFTQTNTPFCLVPPPP
                     LLIKIKDKVPFTVSVSNDTTICPGQSINLIATYLSAQQPVTYNWDNGADLDSIYSVSP
                     TSTTTYNVTITDACGQPLNETVTVYVLPNGPVLTLSDTTICPGQSVTLSAGIAQGAAP
                     FIYNWNNGAANTPTYTVSPNTTTTYSISVTDNCNQQSTGSITVNVSSGPPALTLTPGD
                     TAICPGQSLTLTVNASGAGPYFYNWNNGAAQTASYNVTPANTTTYIITVTDNCAQQVS
                     DTVTVSVLASAPIVAITPNDTTLCKGTTLNLSGTAVNGAGTLIYNWNNGSGNAANYSI
                     TATTSSTVMLAITDQCGNTGMDSIKVTSNDPPQGTVTSADSVCNDNPVLFQFVGTAGS
                     NAAYSWSFVNASPATATGAGPINTTWASAGLQTYTVTVNDGVCPKLELSGEVQINPCA
                     IVIPNVFTPNGDLLNETFFIKNLESFPNTKVKIFNRWGRILLDSNNYKNDWKATDAPD
                     GVYYYTVTIYNGTEFNGTVSILRK"
BASE COUNT          746 a          432 c          511 g          742 t
ORIGIN      
        1 agaaatttga acaaaaatat tttttatagc gcttttcata tattgaatta aagagtgtaa
       61 ataaagggat aaattttttt aaaaaggtga attaataatt atctatagta agagcagtta
      121 gattttcgaa tgaaaatata acttactctt agtaataccg aacaacttca tagcttgttt
      181 ctccaaaacc aaagttgttt cggtataaat gctaaattgt tgaatcgcct aaatcataaa
      241 ctatagtata aaaaaaaaca agcttaactt ttgtgttaag cttgcttcta aaaattggaa
      301 cgaaaaaatt tattttctca aaatagaaac tgttccgtta aactcggttc cgttataaat
      361 tgttaccgtg taatagtaaa ccccatcagg tgcatcggtt gccttccagt cgtttttgta
      421 attatttgaa tcaagtaata ttcttcccca gcgattaaat attttcacct ttgtatttgg
      481 gaagctttcg agatttttaa taaaaaacgt ttcgtttaat aaatcgccat ttggagtaaa
      541 tacatttgga ataacaatgg cacatggatt tatttgaact tcaccgctca actcaagttt
      601 tgggcaaaca ccatcgttaa cggttacagt atacgtttgc aagccggctg aagcccatgt
      661 tgtatttata ggtccggcac ctgttgcagt ggcaggagat gcatttacaa aactccaaga
      721 ataagcagca ttgctgccgg cagttcctac aaattgaaac agaaccggat tatcgttaca
      781 aacagaatcg gccgaggtaa ctgttccttg cggaggatcg ttagatgtaa ccttaatgct
      841 atccatacct gtattgccac attgatcggt aattgcaagc ataactgttg aagatgtagt
      901 tgctgttata gaataatttg ccgcattgcc actaccattg ttccagttat agataagtgt
      961 tcctgcacca tttactgctg ttccgctaag attgagtgtt gtgcctttac ataacgtagt
     1021 gtcgttgggg gtgatagcca ctataggtgc agaagctaat acggaaacag ttacagtgtc
     1081 ggatacttgc tgtgcgcaat tatctgtaac agtaattata tacgttgtag tgttggccgg
     1141 tgtaacatta tacgaagccg tttgtgctgc gccattgttc caattgtaaa aatatggccc
     1201 tgcacctgaa gcatttacag ttaaggtaag cgattgtccc ggacaaattg ctgtatcgcc
     1261 aggagtgagc gtaagagccg gtgggcccga tgacacattt acagtgatac tgccggtact
     1321 ttgttggttg cagttatccg ttaccgaaat agaataagtt gttgttgtat ttgggctaac
     1381 agtgtaagtg ggtgtattgg cagcgccatt gttccagtta taaatgaacg gtgcagcacc
     1441 ttgagcaatt ccagcactta atgtaacact ttggccgggg caaatagtgg tgtcgctaag
     1501 agttaaaacc ggtccattag gcagtacgta taccgtaacc gtttcgttta gtggttgccc
     1561 gcatgcatcg gtaattgtta cgttataagt agtagttgat gtgggcgaaa cactataaat
     1621 tgaatctaaa tcggcaccgt tatcccaatt gtaagtcacg ggttgttgag ccgataaata
     1681 ggtagctatc agattaattg attgtcccgg gcaaatagta gtatcattag aaacagacac
     1741 cgtaaaagga actttatctt ttatttttat gagaagtggt ggaggtggca ctaaacaaaa
     1801 tggggtattg gtttgagtaa aaattatagt ttcggtacct tctgttaaac catcaggagt
     1861 agggtaaatg cttatgaatc ctgaagaatc tccggcagca atttttacac tatcgggtaa
     1921 ttggttgtaa tcaacaccat tcactgctgt accacttata ttcaatttta tatacacatc
     1981 gttaggcaca ggattgggat acgaaaatgt aaccgtcaca tctgtacagc cttctataat
     2041 tgaagaatcg ttggctgcgt atgccgatga aaattgtgcc aatacattgg gaataccacc
     2101 tgtaaaactg ccggcttcga gcaacacgcc cgaatctaaa acaccatcgg ctacatcggc
     2161 aatggctagt tttatatgaa aggtatcaca ggctataaca gcagattgtg cagtaagtac
     2221 agttgtaaat gcatcatatt gcacagtagt gccaccaaag ttatcgtaaa aatatgtgca
     2281 gttcatacaa ggccctgcgt tagcattgcc gttgtttata ttgttgatag aaacaggaag
     2341 ggttgtattg ggaataagtg caatattttt taacccaaca ataccagggc cgctaagaaa
     2401 aaaagcaaac acatcattga aggaagtgtt t
//
LOCUS       DAORQA010000266         2431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9864_length_2431_cov_2.574916, whole genome shotgun sequence.
ACCESSION   DAORQA010000266 DAORQA010000000
VERSION     DAORQA010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9864_length_2431_cov_2.574916"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="PK736_08870"
     CDS             <1..1182
                     /locus_tag="PK736_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33539.1"
                     /translation="GSYKDWKFHAVVLLAAMACFLLFVNNFLPTEIYRTYDYTTFILA
                     VVVIILLYFFRSQNRKLFSFVSAFTVIVVFSIYTAYLVNNLNHEKELQQRILVASKIA
                     EKKDPVAEYLFSDNNEKQLHDKILISYFDELRSESEPGKVFAPQRISQRINQLYFNDA
                     YWSKYEMETYLFNENKQPISENNSFQLYSYNQLVEMIDTLGNSTTAKNFFQINNFSSR
                     NAYLATITITDPDLNNAVNGYAFVLMHEKLGGSKKGFPQLLEKENANAVKINSFDKYS
                     WAFYKNNSLIDKGGKFAFEDNLLIYTNNPPVKNIYYVEQNNVQHLVYKTGANDVLLVT
                     LTDNLRFKLFTEATYWFAFFFMLFLFFWLMMKILNRNLNIEFSFKNRIQFLLWPLCFY
                     R"
     gene            1158..>2431
                     /locus_tag="PK736_08875"
     CDS             1158..>2431
                     /locus_tag="PK736_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC33540.1"
                     /translation="MAIVLLSMILIGSFTFYFINSNYKKNQRLQLLEKLNSLQLVIEN
                     EYDNALVNDFNNDTLSPIFSRTSNALDCDFIIYDKQGVKSYSTKPTLFSLNIIGNIIN
                     RDAYNKLSLNASSSFVQNEMIGNLKYLSSYTGLYDNNNKLLGYINLPYITDPDELKNQ
                     ISEFIITLVNVYVPLFLLCLLLTFLSANQLAKPLQTLQKSMAKVRLGKATEPIVWDKA
                     DEIGALIKQYNKMVDELALSANKLARSERESAWREMAKQVAHEIKNPLTPMKLSVQHL
                     QRAWQDQSPNLMQIVPRLSASIIEQIDTLSAIATEFSNFAKMPTAQFSVVDIQSVLKS
                     SADLFAKEGNSTIEYLVPENLECKVFADKEQLLRVFSNLLKNALQAIPEETNGVIKLN
                     IEKLKTHFLVTIKDNGKGISESEKSKIFVPYFT"
BASE COUNT          815 a          445 c          401 g          770 t
ORIGIN      
        1 ggatcataca aagactggaa atttcatgct gttgttttgc ttgctgccat ggcgtgtttt
       61 ttacttttcg taaataattt tttaccaaca gaaatctatc gcacctacga ctatactact
      121 tttattcttg cagtagtagt tataatcctt ttatattttt tcagatcgca aaaccggaaa
      181 cttttttctt ttgtaagtgc gtttacagtt attgttgtgt tttctattta tactgcatat
      241 ttggtcaaca atctcaatca cgaaaaagaa ttgcagcaac gtattttagt ggcatcgaaa
      301 attgctgaaa aaaaagaccc cgttgccgaa tatttattta gcgataacaa cgaaaagcag
      361 ttgcacgata aaattttgat atcctatttt gatgaattgc gctccgaatc ggaacccggt
      421 aaagtgttcg ccccacaacg catttcgcag cggataaacc agttatattt caatgatgct
      481 tattggagta aatatgaaat ggaaacttac cttttcaatg aaaacaaaca gcctatttcc
      541 gaaaataatt ctttccagtt atattcctac aatcagcttg tagaaatgat agatacgctt
      601 ggaaatagca caacagcgaa aaatttcttt caaataaata atttttcttc ccgcaatgca
      661 tatttagcaa ctattaccat taccgacccc gatttaaata atgctgtaaa cggttatgca
      721 tttgtgttga tgcacgaaaa acttggagga tcgaaaaagg gatttcctca attgctggaa
      781 aaggaaaatg ccaatgctgt taaaataaat tctttcgata aatattcctg ggccttctac
      841 aaaaataatt cgctgattga taaaggcggc aaatttgctt ttgaagacaa tcttcttatt
      901 tatacaaata atccaccggt aaaaaatatt tattatgtcg agcaaaacaa tgttcaacat
      961 cttgtataca aaacaggggc aaatgatgtt ttgctcgtaa cattaacgga caaccttcga
     1021 ttcaaattat ttaccgaagc tacctattgg tttgcatttt ttttcatgtt gtttttgttt
     1081 ttctggctga tgatgaaaat ccttaaccgc aacctcaaca tcgaatttag tttcaaaaac
     1141 cgtatacaat ttctgttatg gccattgtgc ttttatcgat gatactgata ggaagtttta
     1201 ccttttattt tattaatagc aattacaaaa aaaatcaacg tttgcaattg ttagagaaac
     1261 tcaattcact gcaactggtt attgagaatg aatatgacaa tgcccttgtc aacgatttta
     1321 ataacgatac actttcacct attttttcgc gaacatctaa tgccctcgat tgcgatttta
     1381 ttatttatga taaacagggt gtaaaatcat attctacaaa acccacattg tttagtttaa
     1441 atattattgg caatataata aaccgtgacg cttataataa attatcgctc aatgcttcga
     1501 gtagttttgt gcaaaacgaa atgataggaa acttaaaata cctttcttct tataccggtt
     1561 tatatgacaa caacaataaa cttttagggt acatcaattt gccttatata accgaccccg
     1621 atgaattaaa aaatcaaatt tccgaattta tcattaccct tgtaaatgtc tatgtgccgt
     1681 tgtttttgtt gtgcttgttg ctcacctttt tgtcggcaaa tcaattggca aaacccttgc
     1741 aaacattgca aaaaagtatg gccaaagtaa ggttaggaaa agctaccgaa cccatagtat
     1801 gggataaagc cgatgaaata ggggcgctca taaaacaata caacaaaatg gttgatgaac
     1861 tggcattgag tgccaacaaa cttgcacgca gcgaacgcga aagtgcatgg cgtgaaatgg
     1921 caaagcaagt ggcgcacgaa ataaaaaacc cacttacacc tatgaagttg agtgtgcaac
     1981 atttgcaacg tgcatggcaa gaccaaagcc ctaatcttat gcaaatagtg ccacgacttt
     2041 cggcttcaat aatcgaacaa atcgatacgc ttagtgccat tgctaccgag ttttcgaatt
     2101 tcgctaaaat gcccacagct caattttctg ttgtcgatat tcaaagtgta ttaaaatcat
     2161 ctgccgatct ttttgctaaa gaaggcaatt ctacaattga atatcttgtt cctgaaaatt
     2221 tggagtgcaa agtttttgct gataaagaac aactgctacg tgtgttttcc aatctattga
     2281 aaaacgccct gcaagccatt cccgaagaaa caaatggagt gataaaatta aatatcgaaa
     2341 aattgaaaac tcattttttg gtgacaataa aagacaacgg caaaggaatc tcggaatccg
     2401 aaaaatcaaa aatatttgtg ccatacttta c
//
LOCUS       DAORQA010000267         2429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9872_length_2429_cov_4.444398, whole genome shotgun sequence.
ACCESSION   DAORQA010000267 DAORQA010000000
VERSION     DAORQA010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9872_length_2429_cov_4.444398"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1009
                     /locus_tag="PK736_08880"
     CDS             134..1009
                     /locus_tag="PK736_08880"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRC33541.1"
                     /translation="MAESKDNILNRTIRSTYISSIISTSLVLFLLGMLGILLLQANRV
                     KNYVKENHLITIYLQPNLAEADVQAFLTELKQNEAVRTTKYISKEEAAANLQEEIGDD
                     FVTFLGYNPLSACVDVYFKADYVDNSMIDHFKNVMKLKKQVKEVQAQQSLVEQINKNL
                     ESISLVVLIVSCILGFIAVILINNTIRLTLYSKRMIIRSMQLVGATKSFIYKPFLTKS
                     IITGIISAIIAALLLLTFVVLLKKYYSDLIVVNDLQLWSTVVGGLILFGVLLSLICTF
                     FAVGKWLDKPADKVF"
     gene            1049..1318
                     /locus_tag="PK736_08885"
     CDS             1049..1318
                     /locus_tag="PK736_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HRC33542.1"
                     /translation="MVAPLKSTQASTKPAVTNDFAFGKENYMLMIIGIVVLVIGYMLM
                     VGGNPEDPTAFSTDIFSFRRITLAPVVIMIGFAIEVAAIVYKSKE"
     gene            1332..2113
                     /locus_tag="PK736_08890"
                     /pseudo
     CDS             1332..2113
                     /locus_tag="PK736_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584341.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
     gene            2131..>2429
                     /locus_tag="PK736_08895"
     CDS             2131..>2429
                     /locus_tag="PK736_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33543.1"
                     /translation="MSATTPFSLQQFFAGYTLAINKPLRWTSHDVVNKIKIAANNLKL
                     KNEEGQPMKLKIGHAGTLDPLATGLLIICTGRHTKTIDLIQAQTKIYTGTITLGAT"
BASE COUNT          724 a          439 c          455 g          811 t
ORIGIN      
        1 ctcattttca atccttaatt tttaatttct cccccggagt tgttgttcat ttctttttag
       61 atcgtgcatt tttatgagta aatatttttg attcaaaaaa ctatgtttgc cgcttataaa
      121 aaacaatacg tccatggccg aaagtaaaga caacatattg aatcgcacca ttcgctccac
      181 atatatttcg agtatcatca gtacttcgtt ggttttattt ttattgggca tgttgggcat
      241 acttttgttg caagccaatc gtgtaaaaaa ttatgtgaaa gaaaatcacc tcattactat
      301 ttatttacaa cccaatctgg ccgaagccga tgtgcaagcc ttccttaccg aactaaagca
      361 aaacgaagct gtgcgtacta caaagtatat ttcgaaagaa gaagctgctg ccaatttgca
      421 ggaagaaata ggtgacgact ttgttacttt tcttggttac aatccgctga gtgcctgtgt
      481 tgatgtgtat ttcaaagccg actatgtcga caattcgatg attgatcatt ttaaaaatgt
      541 gatgaaacta aaaaagcaag tgaaagaagt gcaggcacaa caatcgctgg ttgagcaaat
      601 aaataaaaac cttgaatcta tttcgctcgt agtgcttatt gtaagttgca tacttggttt
      661 tattgctgtt atacttatta ataataccat caggcttaca ttgtattcca agcggatgat
      721 tattcgcagc atgcaattgg tgggcgccac caagtcgttt atttacaaac catttttaac
      781 aaaatctatt atcaccggca tcataagcgc aataattgct gcccttttat tattgacatt
      841 tgtagtgctg ttaaaaaaat attattccga tttaattgta gtaaacgacc ttcagttatg
      901 gtcgacagtg gtagggggat taattttatt tggtgtttta ctttcgctaa tttgtacttt
      961 ctttgcggtt ggcaaatggt tagacaaacc tgccgataaa gtattttaaa acacaaataa
     1021 aatggctaca caacaaggca aaaaaaatat agttgcacca ttaaaatcta cgcaagcaag
     1081 cactaagcct gcggtaacaa acgatttcgc ttttggcaaa gaaaattata tgctaatgat
     1141 tatcggcatt gtagttcttg ttattggcta tatgcttatg gtgggtggca atcctgaaga
     1201 ccctacagca ttcagtaccg atattttttc ttttcgtaga attactttag cgcctgttgt
     1261 tattatgatt ggttttgcaa tagaagttgc tgccatcgtt tacaaatcga aagaataact
     1321 cccgtttttt tatgacttat ttagaggcta ttattattgc cattgtagaa ggcattaccg
     1381 aatttttgcc catatcgtcc accggccata tgattattgc cgaggcattt ttgaaagtag
     1441 acgatttgca atttgccaaa ctctttaccg tttgcattca gtttggagcc atactttcgg
     1501 ttgtggtttt atactggaag cgattttttc aaacactcga cttttattac aaactatttg
     1561 tagcttttat tcctgctgtt gtttgcggat tattatttaa cgatgccatc gatggtttgt
     1621 taggcaatgt tactgtggtg gcattcacat tatttattgg cggtattgtt ttattgtttg
     1681 tagataaatg gttcaaaacg cgggaggcat taccagaaat gccctggtaa taggattgtt
     1741 tcaatgcata agcatggtgc ccggagtgtc gcgttcggct gcaactatta taggaggaat
     1801 gacgcagaag cttacacgaa cacaagctgc ggagttttcg ttttttcttg ctgttccaac
     1861 aatgtttgct gccacagcaa aaaaactact cgattattat caggaaggaa tttcattaac
     1921 aggtaatcag ataaatttgc ttatcgtagg aaatattgtt gcgttcatag ttgcattgct
     1981 ggccataaaa tcattcatag gttttttatc gcgcaacggt ttcaaagcat ttggatatta
     2041 ccgcattatt gttggcgctg ccttattgat tttgattttt gttttcaaag tagatttaaa
     2101 attggttgat taattcaaag gaaaaatctc ttgtcagcca caacaccatt ttcgttacaa
     2161 caattttttg ccgggtacac acttgccatt aataaaccgc tgcgttggac atcgcacgat
     2221 gtggtaaaca aaataaaaat tgccgccaat aatttaaaac ttaaaaacga agaaggccaa
     2281 cctatgaaac tgaaaatagg acatgccgga accctcgatc ctttggcaac aggattactt
     2341 attatttgta ccggcaggca caccaaaacc atcgatctca ttcaagcaca aacaaaaatt
     2401 tatacaggta caataacatt gggcgctac
//
LOCUS       DAORQA010000268         2426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9887_length_2426_cov_13.350907, whole genome shotgun sequence.
ACCESSION   DAORQA010000268 DAORQA010000000
VERSION     DAORQA010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9887_length_2426_cov_13.350907"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PK736_08900"
     CDS             <1..723
                     /locus_tag="PK736_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33544.1"
                     /translation="CAGPVAALASSDTLWCDKTCIDFFDLSQNNPTSWTWYFQGASPS
                     TSTDQNPTGICYNNYGSFDVTLVACNALSCDSLYLDDFVTEFQLPAPSVVTFSNDTLY
                     STPAFSYQWYNTNNINLVLGTLNYFVPVVDGSYFVLTSDSNGCSVPSNTVGFYTGINN
                     ISHSQQFTLSPNPANNILQITTEQTGTYQCSIIDYTGRQVMNFQSPAQIQKVDISKLS
                     KGIYSVLITTNNQVVQLSFVKQ"
     gene            739..>2426
                     /locus_tag="PK736_08905"
     CDS             739..>2426
                     /locus_tag="PK736_08905"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC33545.1"
                     /translation="MYKSLTVLVIVFCIHTFSIAQKQYNHWFFGTNCGLDFSAGAPVF
                     QAGGQISTDEGCSAMSDKNGNLMMYTDGLKVWNANHVIMPNGSGLMGTSSSTQAALIV
                     PAPGSTTLFYIFTTATGGDAAYNVVDMTLNGGLGDVTTKNVVLFSNTTERVCAIKHAN
                     GVDYWIVFHGLNTNEYKAFLLTSAGVNAVPVNTNIGTAHSGGGDEIGYMKSNYQGTKI
                     CIGVRYQDLYDLLDFDNATGVLANLITLTPYPYAYGVEFSLDGKILYVSNGLAAGTIH
                     QFDLTSNNAATIIASDYLVANISSGTNGGIQMGPDLKIYQTAWYNYDLGCINNPDVLG
                     AGCNFVGNQIVMPNQGRGGLPNCVPDAFKQVSVTNLCFGDTTFFTLNDTGGILVASWN
                     FNDPNSGNNTSSNINTSHLFSAPGNYSVQFITVNQSLASDTQYIDVTIIAPPVISLGN
                     DTTLCNGQSLLLNAGNGFSNYLWQNGNTTATYTATVSGNYNVNVISVDGCKASDSIDV
                     TFIVCAGPVAALSSSDTLWCDKTCIDFFDLSQNNPTSWTWYFQGASPSTSTDQNP"
BASE COUNT          695 a          498 c          485 g          748 t
ORIGIN      
        1 tgtgcaggcc ctgtggcggc attagcatca agcgatacat tgtggtgcga taaaacgtgc
       61 atcgactttt tcgacctttc gcaaaataat cctacttcat ggacatggta ctttcagggg
      121 gcttcgcctt ccacttctac cgaccaaaac cctaccggaa tttgttacaa taactatggc
      181 agtttcgatg ttactttggt agcgtgcaat gctttaagtt gcgattcgct ttacctcgat
      241 gattttgtaa ccgagtttca attacctgcg ccatctgtag taacttttag taacgacacc
      301 ttgtattcca ctcctgcttt ttcatatcaa tggtacaata caaataatat aaatcttgta
      361 ctgggcacgc tcaattattt tgtgcctgta gtagacggaa gctatttcgt tttgacaagc
      421 gatagtaatg gatgcagtgt tccttctaac accgtaggtt tttataccgg aataaacaac
      481 atatcgcact cacagcaatt tactttaagt cctaatccgg caaacaacat tttgcaaatt
      541 actactgaac aaacaggcac gtatcaatgt tcaattattg attataccgg ccgccaggta
      601 atgaattttc aatcgcctgc acaaatccaa aaagtagaca tatcgaaact ttctaaaggg
      661 atttattctg tacttattac taccaataat caggtagttc aattgtcatt cgtaaaacaa
      721 taaataattt ttttagccat gtataaatca ttaaccgttc tcgtcattgt attttgtatc
      781 cacacatttt caattgctca aaagcaatac aaccattggt ttttcggcac taactgcggg
      841 ttagattttt cggccggagc accggtattt caagccgggg gacaaatttc taccgatgaa
      901 ggatgctcgg caatgtcgga caaaaatggt aacttaatga tgtatactga cggcctgaag
      961 gtttggaatg cgaaccatgt tataatgcca aatggctctg gtttgatggg aacatcatcg
     1021 tccacacaag cggcattaat agttcctgcg cccggcagca caacactatt ctatattttt
     1081 actactgcca ctggtggcga tgcagcttat aacgttgttg atatgaccct caatggagga
     1141 ttgggcgatg tgacgacaaa aaatgtggtt ttgttttcga acactaccga gcgggtgtgt
     1201 gccataaaac atgctaatgg tgttgactat tggattgttt ttcatggtct taataccaat
     1261 gagtataaag cctttttgct aacatcggcc ggagtgaatg ctgtaccggt aaatacaaat
     1321 attggtactg cacatagcgg aggtggcgat gaaattggat atatgaagtc caattatcaa
     1381 ggcacaaaaa tttgtattgg agtgcgctat caggatttat atgacttact cgattttgat
     1441 aatgctacag gcgttttagc gaacttaatt accttgacac cttaccctta cgcatatggc
     1501 gtggagtttt cgctcgatgg taaaatttta tatgtcagca atggcctggc tgccggcact
     1561 attcatcaat tcgatttaac ttcaaataat gctgcaacaa ttattgcatc cgattatttg
     1621 gtggccaata tttcttcagg aacaaatggt ggaatacaaa tgggtcccga tttaaaaata
     1681 tatcaaacag cgtggtacaa ttatgacctt ggatgtataa acaaccccga tgtgttgggt
     1741 gcaggatgta actttgttgg caaccaaatt gtaatgccta atcagggacg aggcggattg
     1801 cccaattgtg tacccgatgc attcaaacaa gtaagtgtta ccaatctttg ttttggcgat
     1861 accacttttt tcaccttaaa cgataccgga ggcatacttg tagcatcgtg gaattttaat
     1921 gatcccaata gtggcaacaa tacttcttct aacattaata catcgcactt gttttcggct
     1981 cccggcaatt acagcgtgca gtttattact gtaaaccaaa gccttgccag cgacacgcag
     2041 tatattgatg ttactattat tgcccctcct gtaattagtt taggaaacga tacaacgttg
     2101 tgtaatggcc aatcgctttt gttaaatgcc ggaaatggat tttcaaacta tctttggcaa
     2161 aatggaaata caactgctac gtatacagct acagttagcg gcaactataa tgtaaatgtt
     2221 atttctgtag atggatgtaa agccagcgat tcgatagatg tgacctttat agtttgtgcc
     2281 ggccctgttg cggcattatc atcgagcgat acattgtggt gcgataaaac ctgtatcgac
     2341 tttttcgacc tctcacaaaa taatcctaca tcgtggacat ggtactttca gggtgcttcg
     2401 ccatcaacat ctaccgacca aaaccc
//
LOCUS       DAORQA010000269         2404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_9993_length_2404_cov_7.420179, whole genome shotgun sequence.
ACCESSION   DAORQA010000269 DAORQA010000000
VERSION     DAORQA010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9993_length_2404_cov_7.420179"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>298
                     /locus_tag="PK736_08910"
     CDS             <1..>298
                     /locus_tag="PK736_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33546.1"
                     /translation="CAVINLNLQFCFGQTNISGIVNTYVEATSVTCNTISVASTAGFF
                     PNDRVLIIQMQGAIIDTTQTANFGNITNMANCGNYEFANIAIVNGNNISLKRSLK"
     assembly_gap    299..325
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <326..>2404
                     /locus_tag="PK736_08915"
     CDS             <326..>2404
                     /locus_tag="PK736_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRC33547.1"
                     /translation="QIIKVPHYTSANVTGLVTSPPWNGATGGVVVFEVDNTLSLASNI
                     DVSGAGFRGGSATNNPDNNCGTGSNLYAYDVNQGGAIWNEGGAQKGEGIAILPNTQNA
                     GRGKAGTGGGGGNKHNAGGGGGSNYTEGGTGGNDNCANAVGGSGGASVATNFFQNKIF
                     FGGGGGCGDANDGAGTPGTNGGGIIIIKAADISGNGFAIKANGADQLTSAAGPGDGGG
                     GGGGAGTILLDVPTYTSIVVVEAKGGKGGDVNPSTAACFGPGGGGGSGLIWIVPGFVP
                     PNLSPNTSAGLPGNDLAAISPCFNTTNNATAGTNNAQNNFLNNLSLQESFVNYAGIIN
                     LGNDTTLCNGDTIILNGGLNANLFLWQDGSTNMTYTVTTPGIYILNVVDGNYCAASDT
                     IDIATPNSFIYDLGNDTTLCNGQSLVFDLTSLAANYQWSNGSTNPIFTISTAGSYTVT
                     VTQNGYCTLIDSIKVIFINGFNVDIGNDTTLCKNETLTLDAGNPGLTYNWSTGEATQQ
                     ITITAPGNYFVEVTDASCLIIDSININYRFLNIELGTDTFVCNGSSIPFAFDTTYKYK
                     WSDGSTKSTFNITTAGTYWVVAKDSTCAETDTIQIEEYSSTEVFVPNAFTPNDDATNE
                     LYKPATDYLEKYLFRIFDRYGHMVFETADPLAGWDGTFLDQPAPEGMYAYTLTYYSPC
                     SQYKIKTCEKF"
BASE COUNT          700 a          492 c          529 g          656 t
ORIGIN      
        1 tgtgcagtta ttaatcttaa tctgcagttt tgttttggac aaacaaatat ttcgggcatt
       61 gtaaatactt atgttgaggc taccagtgtt acatgcaata ccatctctgt agcatcaact
      121 gcgggctttt ttcctaacga ccgtgtactt attatacaaa tgcaaggtgc tattatcgat
      181 actacccaaa cagcaaactt tggcaacatt accaacatgg ccaattgcgg aaactacgaa
      241 tttgcaaata ttgcaattgt aaatggcaac aatatttcac tcaagcgatc attaaaaann
      301 nnnnnnnnnn nnnnnnnnnn nnnnncaaat aataaaggtg ccacattaca ccagtgccaa
      361 cgttaccggc cttgtaacca gtccaccttg gaatggcgca accggtggtg tggtagtttt
      421 cgaggttgac aatacgcttt cgctggcttc aaatattgat gtaagcggtg caggttttcg
      481 tggtggaagc gcaaccaata atcctgataa taactgtggc accggcagta atttatatgc
      541 atatgatgtt aaccagggag gagcaatatg gaacgaaggc ggtgcccaaa aaggagaagg
      601 cattgccata ttaccaaaca cacaaaatgc agggcgaggt aaagccggaa ctggtggtgg
      661 cggtggtaac aagcacaatg caggtggtgg cggtggcagc aattacacag aaggcggtac
      721 aggtggaaac gataattgcg ccaatgcagt aggtggaagt ggtggtgcaa gtgtggcaac
      781 aaattttttt caaaataaaa tatttttcgg tggtggtggt ggctgtggcg atgccaatga
      841 cggtgccgga actcccggta caaacggtgg tggcatcatc ataattaaag ccgctgatat
      901 ttccggtaat ggctttgcca taaaggcaaa tggtgccgat caattaactt ctgcggccgg
      961 ccctggcgat ggtggcggtg gtggtggcgg tgccggcact attttgctcg atgtgcctac
     1021 gtatacatct atagttgttg tagaagctaa aggcggaaaa ggtggcgatg ttaacccttc
     1081 aactgctgcg tgctttggcc ccggtggtgg tggtggcagc gggctaatat ggattgtacc
     1141 cggttttgta ccgcccaatt tgtcgcctaa taccagtgcg ggtttgcccg gtaatgatct
     1201 ggcagctatt tctccatgct tcaatactac caacaatgca acagccggaa caaacaatgc
     1261 acaaaacaat tttttgaata atctatcgct gcaagaaagc tttgtaaact atgccggcat
     1321 catcaatctt ggtaacgata ctacactttg caatggcgat acaattattt taaacggtgg
     1381 attgaatgct aatttatttc tatggcaaga tggttctaca aatatgacat acacagttac
     1441 cactccggga atctatattt tgaatgtggt agatggaaac tattgtgctg cctccgatac
     1501 tattgatatt gcaaccccta acagttttat atacgacctt ggtaacgata caacattatg
     1561 caatggtcaa agtttggttt tcgatttaac atcgcttgcc gcaaattacc aatggagcaa
     1621 tggaagtaca aatccaattt ttactatttc tactgccggt tcgtatacgg ttactgttac
     1681 ccaaaatgga tattgcacac ttatcgattc tattaaagtg atttttataa atggatttaa
     1741 tgttgatata ggaaacgata ctacactgtg caaaaatgaa accctcacac tggatgcggg
     1801 caacccggga ttaacctaca actggagcac gggcgaggcc acacaacaaa ttactataac
     1861 tgcaccggga aattattttg tagaggtgac cgatgccagt tgtcttatta ttgattctat
     1921 aaatatcaat tatcgttttc ttaatatcga attgggtacc gatacttttg tgtgtaacgg
     1981 tagtagtata ccctttgctt ttgataccac ttacaaatac aaatggagcg atggcagcac
     2041 aaaatcaacc ttcaacatca ccacagcagg cacttattgg gtagtggcaa aagatagtac
     2101 ttgtgccgaa accgatacca tacaaataga agaatactcg agtacagaag tttttgtgcc
     2161 caatgcattt acaccaaacg atgatgccac caacgaatta tataaaccgg caaccgatta
     2221 tttagaaaaa tatttgttcc gcattttcga ccgttatggc cacatggttt ttgaaactgc
     2281 tgacccgctt gcaggctggg atggcacttt cctcgaccaa cccgcccccg aaggaatgta
     2341 tgcatacacc ctcacctatt actctccttg cagccaatac aaaatcaaga cctgtgaaaa
     2401 gttt
//
LOCUS       DAORQA010000270         2397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10019_length_2397_cov_6.839880, whole genome shotgun sequence.
ACCESSION   DAORQA010000270 DAORQA010000000
VERSION     DAORQA010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10019_length_2397_cov_6.839880"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(358..1884)
                     /locus_tag="PK736_08920"
     CDS             complement(358..1884)
                     /locus_tag="PK736_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC33548.1"
                     /translation="MFQQNIPFKEITTAVAGRKKYKRAEFNICISDVDHIFARHLIEF
                     YLRMCGCTKFGWLDDNKKFLPASNLISNALCLFRDVLIWPFQLLRYKMLLNKLEQTAF
                     KKYLPFNKSQVVINYLRTDHWFNLRSGGSVGHVSGVINAMTKAGKLNTIFSSDNLFEV
                     QQTELLVKISPRYNVGSSIPELPQMNYNFQLIDKLKNEERFKGSAIYQRYSQYNVTGV
                     YLKYHFAIPLILEFNGSEVWAIKNWGSGKILHFNFLVRCEKINLMHADLIVVVSQNLK
                     DSLMTEGVDASKILVNPNGVSLQKFNEQVNGNVIREKYSLQNHKVIGFIGTFGEWHGV
                     IEMAKAIVIFFDAHPEEISKVKFLLIGDGNLLESVRGIIYQSSYRDTVIFTGKVNQNE
                     SPQYLAACDILLSPHIGNPDGSKFFGSPTKLFEYMAMRKPIIASNLEQIGDILTHSQS
                     AFLVPPGDVAALANAFFHLLKNETLQQTLAQGAYNKVIENYTWDMHVNKILEAGGGEL
                     "
     gene            complement(1959..>2397)
                     /locus_tag="PK736_08925"
     CDS             complement(1959..>2397)
                     /locus_tag="PK736_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HRC33549.1"
                     /translation="IGKRNVSTDGYNIGMQYQCVEFVKRYYYEYYHHKMPDSYGNAKD
                     FFDNTLADGVYNGNRDLLQYRNSSKAKPQQGDLVIMDATRFNPYGHVAIVSNVTNNSV
                     EIIQQNPGPAALSRVKYDLQYRKNLWRIDNARILGWLRVKTKN"
BASE COUNT          740 a          492 c          397 g          768 t
ORIGIN      
        1 gttattggtt attgagttat tgggttattg gttgtgactt tgttatgctt aggaacgaag
       61 catctctgca aagaatggag taaggtcatc gtcattctga gcgaagtcga agaagcattg
      121 gtcatttttt atttgcgtca gccattattt gtaaccccaa aaacaatggt attctatttc
      181 gtttatacta ataatcaatt ctaaattcca aatttctgtc tcccaattgt aaatacaaaa
      241 ttcacaactc accattccca aattcacaaa ttcacaactc acaactcaca attcacaatt
      301 cacaactcac aattcccaaa ttcacaaatt cacaactcac aattcaccat tcaccattca
      361 caattccccc cctccggctt caagaatttt attcacatgc atatcccacg tgtagttttc
      421 aattactttg ttgtaagcac cttgtgctaa agtttgctgt aaagtttcgt ttttgagcaa
      481 atgaaaaaag gcatttgcca aagcagcaac atcgccaggt ggtacaagga atgccgattg
      541 gctatgcgta agaatatcac caatctgttc gaggttactg gcaataatgg gtttgcgcat
      601 ggccatatac tcgaacagtt ttgtggggct gccaaaaaat ttacttccat cgggattgcc
      661 aatgtgtgga gacaataaaa tatcgcaagc cgccagatat tgtggacttt cgttttgatt
      721 caccttgccg gtaaatataa ctgtatccct atacgatgat tgataaataa tgccacgaac
      781 cgattcgagc aaattgccat cgccaatgag taaaaatttc actttagata tttcttcagg
      841 atgagcatcg aaaaaaataa caatagcttt ggccatttct attacgccat gccactcgcc
      901 aaatgtgcca atgaaaccaa ttactttgtg attttgtaaa ctatattttt cgcgaataac
      961 atttccgttc acctgctcat taaatttttg caacgaaaca ccattaggat tcacaagaat
     1021 ttttgatgca tcaactcctt ctgtcataag gctatcttta aggttttgcg ataccaccac
     1081 aatcaaatcg gcatgcatta aatttatttt ttcgcaacgc accaaaaagt tgaaatgcaa
     1141 aatcttacca ctaccccaat tttttattgc ccacacttcg ctgccattaa actcaagaat
     1201 caaaggaatt gcaaaatgat attttaaata cactccggtt acgttatatt gactatatcg
     1261 ctgataaatg gcactgcctt taaaccgctc ttcgtttttt aatttatcga tgagttgaaa
     1321 attgtaattc atttggggca attcgggaat ggatgaacca acattgtaac ggggagaaat
     1381 ttttacaagc aattcagttt gttgaacttc aaacaaattg tcggacgaaa aaatggtatt
     1441 caactttcct gcttttgtca tggcattaat tactccgctt acatgaccaa cactgccacc
     1501 actgcgcaaa ttgaaccaat gatcggtgcg gagataattt attacaactt gcgatttgtt
     1561 gaagggtaaa tattttttga aagcagtttg ctcaagctta tttaaaagca ttttataacg
     1621 aagcaattga aacggccaaa tcaaaacatc gcgaaaaaga caaagagcat ttgaaattaa
     1681 atttgatgca gggaggaatt tcttattgtc atcgagccag ccaaattttg tgcaaccaca
     1741 catgcgcaaa taaaactcta taaggtggcg ggcaaaaata tggtctacat cgcttatgca
     1801 aatattaaac tctgcacgtt tatatttttt gcgccctgct acggcagttg taatttcttt
     1861 gaaaggtata ttttgctgaa acaatcttta atttctttgg cgcgaattta taaacatttt
     1921 caatggacac atattgatgc tgaagcaaaa tgagttaatt aatttttggt tttcactcgc
     1981 agccatccca atatgcgtgc attatctata cgccataaat tttttcgata ttgcaaatca
     2041 tactttactc tcgataacgc tgcaggccct ggattttgtt gtattatttc cacgctgtta
     2101 ttggtaacat tcgatactat agcaacatga ccataaggat tgaacctggt ggcatccatt
     2161 atcaccaaat cgccttgctg tggctttgcc ttactgctat tgcggtattg caacaaatcg
     2221 cgattgccat tatatacacc atcggcaaga gtattgtcga aaaaatcttt tgcattgcca
     2281 taactgtccg gcattttatg atgatagtac tcgtagtaat atctttttac aaattccaca
     2341 cattgatact gcattccaat attgtagcca tccgtgctta cattccgttt gccaatg
//
LOCUS       DAORQA010000271         2394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10039_length_2394_cov_7.663959, whole genome shotgun sequence.
ACCESSION   DAORQA010000271 DAORQA010000000
VERSION     DAORQA010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10039_length_2394_cov_7.663959"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PK736_08930"
     CDS             <1..737
                     /locus_tag="PK736_08930"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRC33550.1"
                     /translation="INGLIGTRNSPPLFNLAFQKDFMWDGGVNNLEVQPLLPITNPVE
                     MDEDINNVVAKLDKLPTYRQLFKSAFGDETINSQRVLRALSQFMVMMISANAKYDKVK
                     LGKETFSTAEQSGYDVFVQKCSACHQEPLFTDLSFRNNGLDLFPKDPGRAMITFDPAD
                     SGKVKVPSLRNLKYTYPYMHDGRFQNLQEVIMHYTAGVKPCATLDPLLSNGITLSNTE
                     IDDLLTFLKTLDDVDFTKDKKLSEYQ"
     gene            743..1519
                     /locus_tag="PK736_08935"
     CDS             743..1519
                     /locus_tag="PK736_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33551.1"
                     /translation="MTKSNNNISTFLYLVMLFIFVGGCKKKEEEPPADEKTYGNIVLQ
                     FKHLVGDSALVYPSKEYINVLGQPYSVSKLKYYVSNIKLVKPAGGKTEVSGYYLLEHS
                     NTSTSNIYIGSVATGTYVGVEFNIGVDSTTTVSGNHTGALSHTNGMFWNSTDGFIFLK
                     MEGSSVESPTGNFQFDIGGYSAPVNNFKKIIPPFNGKTIEVQKEHTSYVNFKTDVAEI
                     LSNPYSINFYYTYNILAPNGDSQKIAKNYMDMFSVMSVIN"
     gene            1767..1856
                     /locus_tag="PK736_08940"
     tRNA            1767..1856
                     /locus_tag="PK736_08940"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1801..1803,aa:Ser,seq:tga)
     gene            complement(1992..>2394)
                     /locus_tag="PK736_08945"
     CDS             complement(1992..>2394)
                     /locus_tag="PK736_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33552.1"
                     /translation="TNAVIHDIPGGRFFISRDSRFVFSVYDSDRNGFAVFDMRLCKTA
                     YLNDSLATPVAQWYFQGGKYFSIDEEADDEADDESESFNPNGLMQFTIQTFDFAKRSL
                     KQEKLPFENLKEESKIKLFPEIKNITDCQCK"
BASE COUNT          756 a          437 c          446 g          755 t
ORIGIN      
        1 gtattaatgg tttaatagga acacgcaatt cgccaccact tttcaattta gctttccaaa
       61 aagattttat gtgggatggc ggtgtaaata atctcgaagt acagccttta ctgcctatta
      121 caaatcctgt agaaatggat gaggacataa ataacgttgt tgctaaactt gataagttac
      181 ccacttacag acaattattt aagagcgctt ttggtgacga aaccatcaac tcgcaaaggg
      241 tattgcgggc actgtcgcaa tttatggtaa tgatgatttc ggcaaatgca aaatacgata
      301 aagtgaaact tggaaaagaa acattttcta cggctgagca atcgggatac gatgtatttg
      361 tacaaaaatg ttcagcatgt catcaggaac cattattcac cgatttaagt tttcgtaata
      421 atggtttaga tttatttcct aaagatcccg gtagagcaat gattactttc gatcctgccg
      481 attcgggcaa agttaaagtg ccttcgttgc gcaatctaaa gtatacttat ccatatatgc
      541 atgatggacg ttttcaaaat ctacaggaag ttattatgca ttacactgca ggtgtaaagc
      601 cctgtgccac gttggatcct ctgctttcaa atggcataac attatcaaat accgaaattg
      661 atgatctgct aacttttcta aaaacattag atgatgttga tttcaccaag gacaaaaaac
      721 tatcagaata tcaataaata ttatgacaaa aagcaataac aatatttcaa ctttcttgta
      781 tttagtaatg ctgtttattt ttgtgggcgg gtgcaaaaaa aaggaagaag aaccgcctgc
      841 cgatgaaaaa acatacggta atattgtgtt gcaattcaaa catttagttg gtgatagtgc
      901 cttggtttac ccttcgaaag aatatatcaa tgtgcttggg caaccttatt cagtttcgaa
      961 actgaagtat tatgtaagta atattaaact ggttaaaccg gcaggtggaa aaacagaagt
     1021 aagcggttat tatttactcg aacattccaa cacttcaact tcaaatattt acataggctc
     1081 ggtagctacc ggaacgtatg ttggtgtaga attcaatatt ggagtggata gtacaactac
     1141 agttagcggt aatcataccg gagccttaag ccatacaaat ggaatgttct ggaacagtac
     1201 ggatggtttt atttttttga aaatggaagg ctcatcagtg gagtcgccaa caggaaattt
     1261 tcaatttgat attggtgggt acagcgctcc ggtaaataat ttcaaaaaaa taattcctcc
     1321 tttcaatggc aaaacaattg aagttcagaa agagcatact tcctatgtta acttcaaaac
     1381 agatgtagct gaaatcctgt caaaccccta ctctatcaac ttttattata catacaatat
     1441 tttggcgcca aatggtgatt ctcaaaaaat tgctaaaaat tatatggata tgttctctgt
     1501 gatgtctgtg atcaattagt attttgttgt cacgcggtga ctaatattta atttatccct
     1561 ccaagtgtct ttctccagtc tccttcagtc tgcgcaagct ttgtcatcga aacccgctaa
     1621 gaagtttgga atcatgtttt tcaaaattac tttgtaactg aaaggacata ttttaaatca
     1681 gcactctaat ttaaactctc tgaaaagtag aatattaagg gtgatatttt ttttcgaatt
     1741 attttggcac tacaatcttc gcccccggag agttggcaga gcggtcgatt gcggcagtct
     1801 tgaaaactgt tgaggtgtta cagcctcctg gggttcgaat ccctaactct ccgccaaaaa
     1861 tttagccttg cttttgcaag gcttttttgt tttcagtgat ttggttgatg tttggaatgg
     1921 ataaataagt aaagcatgac ccagcgcatt tgtttattac ctttcaatat ataatagcac
     1981 caaagacttt ttcacttaca ttgacaatct gtaatattct taatctcggg gaaaagtttt
     2041 attttacttt cttctttcaa attttcgaat ggcagttttt cctgtttcaa actgcgcttg
     2101 gcaaaatcga aagtctgaat ggtaaattgc ataagcccat tagggttaaa agattcgctt
     2161 tcatcatcgg cttcatcatc ggcttcttca tcaatgctaa aatattttcc gccctgaaag
     2221 taccattgcg caaccggagt tgctaaagaa tcgttcaaat atgcagtctt gcacaaccgc
     2281 atatcgaaaa cagcgaagcc atttctatcg ctatcataaa ccgaaaaaac aaaacgacta
     2341 tcgcgcgaaa taaaaaatct tccaccggga atgtcgtgaa tcacggcatt ggtg
//
LOCUS       DAORQA010000272         2390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10067_length_2390_cov_6.284368, whole genome shotgun sequence.
ACCESSION   DAORQA010000272 DAORQA010000000
VERSION     DAORQA010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10067_length_2390_cov_6.284368"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PK736_08950"
     CDS             <1..769
                     /locus_tag="PK736_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33553.1"
                     /translation="FGCDSFVTTQLIVNQNYNIISSASICSGDSIFIGGAWQFNAGNF
                     VDNYSTLFGCDSIITTVVTVNPIYYSISDISICTGDSIFIGGAWQNVQGNYYDIFSTK
                     LGCDSIFETALNINPVFNFTDTITILLGDSVFAGGAWQTTAGVYTDYYITQNSCDSIV
                     TTVLNVVTGIAEYLSSTFINIKPNPGNGIFTIIFTSSEIKPSQLLIKNIQGQQLFIDV
                     IISNQAKLIDITSLANGVYILQVNTLTRSYYYKLIKK"
     gene            complement(731..985)
                     /locus_tag="PK736_08955"
     CDS             complement(731..985)
                     /locus_tag="PK736_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33554.1"
                     /translation="MPIKKTKENEDSKAGSYWFFAFAFGKCFHWPKNSPTTAIQAEPF
                     CENENIVLKKILPLVGFSLTKNVTIITDSLLNQLIIIASR"
     gene            complement(1040..>2047)
                     /locus_tag="PK736_08960"
     CDS             complement(1040..>2047)
                     /locus_tag="PK736_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRC33555.1"
                     /translation="GFTAKDLVTDQDIRWCPGCGDYSILKQAQTVIPELGIPRENIVF
                     ISGIGCSSRFPYYMETFGMHSIHGRATAVASGLKATRPELSVWIITGDGDALSIGGNH
                     FIHMLRRNFNVNVLLFNNQIYGLTKGQYSPTSEEGKITKSTPYGSLDHPFNPIALAMG
                     ADGTYIARSMDRDPVHLKDTLRKANAHKGTSFVEIYQNCNVFNDGAFEIFTEKSSKKI
                     ETLMVENGKPFIFGANNEKGIKLDGFKPVIVDLTDGNSANDLWVHDETDRVKAGILSR
                     FFDNPAKEGHFPRPFGVFYKEERNCYEDDLNQQVADVLAKRGEGNLDALLRGREVWEI
                     K"
     assembly_gap    2048..2078
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2132..>2390)
                     /locus_tag="PK736_08965"
     CDS             complement(2132..>2390)
                     /locus_tag="PK736_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HRC33556.1"
                     /translation="MAVIQARAKGYDVSHAQVRYMNPFPKNLGEILYGFEKILVPEMN
                     TGQFLSMLRSKYLVPAEGLNKIKGIPFSSVEIMEKIESMIA"
BASE COUNT          699 a          461 c          419 g          780 t
ORIGIN      
        1 atttggatgt gattcgtttg ttactacaca actaatcgta aatcaaaatt ataacattat
       61 ttcatcggct tctatttgct cgggcgatag catttttatt ggtggtgctt ggcaatttaa
      121 tgcaggcaac tttgtagata attattcgac actttttggt tgcgactcca tcatcacaac
      181 tgtagtaaca gttaatccaa tttactattc catttcagat atttctattt gcactggaga
      241 tagtattttt attggaggtg cttggcaaaa tgtgcaagga aattattacg atatattttc
      301 aaccaaatta ggttgcgatt caatatttga aactgcatta aacataaatc ctgtttttaa
      361 ttttacagat acaataacta ttttgttagg cgatagtgtt tttgctggtg gagcttggca
      421 aactactgca ggtgtgtata cagattatta cataacccaa aatagttgtg atagtattgt
      481 aaccacggtg ctcaatgttg taacaggaat agcggaatat ttgagctcaa catttatcaa
      541 cattaagcct aatcctggca atggcatttt tacaataatt tttacttctt ccgaaataaa
      601 accttctcag ctattaataa agaacatcca aggccaacaa ttattcattg atgttataat
      661 atcgaatcaa gcaaaactaa ttgacattac ttcgctcgcc aatggtgttt atattctgca
      721 agtaaacact ttaacgagaa gctattatta taagttgatt aagaagtgag tcggttatta
      781 ttgtgacatt tttagtaaga gaaaaaccga ccaaaggcaa aatttttttc aaaacgatat
      841 tttcgttttc acaaaaaggt tccgcctgaa tggctgttgt cggtgaattt tttggccagt
      901 gaaaacactt accaaaggca aaagcaaaaa accaataaga acccgcttta gagtcctcat
      961 tttcttttgt ttttttaatt ggcatgaaag actgtaaagt taactaaaaa ctcagaacta
     1021 aaaactaaaa acttcctccc tattttatct cccaaacttc tctgcctctt aataaagcat
     1081 cgagatttcc ttcgccacgt tttgctaaaa catctgccac ttgttggttc aaatcgtctt
     1141 cgtagcaatt gcgttcttct ttataaaaca caccaaaggg gcgtgggaag tgcccttctt
     1201 ttgcagggtt atcaaagaag cgagagagga ttccggcctt tactctatct gtttcatcgt
     1261 gcacccacag gtcgttggcg ctgttgccat cggtaaggtc aactatcaca ggtttaaaac
     1321 catcgagctt aattcctttt tcgttattgg caccaaaaat aaaaggctta ccattttcaa
     1381 ccataagggt ttcgattttc ttcgaagatt tttcggtaaa tatttcgaaa gcgccatcat
     1441 taaacacatt gcagttttga tatatttcga caaacgatgt gcctttgtgt gcattggctt
     1501 ttcgcagggt atctttcaag tgtaccgggt cgcggtccat actacgtgca atgtaagtac
     1561 catcggcacc catggctaaa gcaataggat taaaaggatg atcgagcgaa ccataaggag
     1621 tagattttgt aatttttcct tcttcgctcg ttggagaata ttgccctttt gttaacccat
     1681 aaatctggtt gttgaacaac aaaacattga cattaaaatt tctgcgcaac atgtgaataa
     1741 aatgattgcc gcctattgat agtgcatcgc catcaccggt aataatccac acactcaatt
     1801 cgggacgagt ggccttgagg cccgatgcca ctgcagttgc gcgcccatgt atactgtgca
     1861 tgccaaatgt ttccatatag tatgggaagc gcgatgaaca tccaatgccc gatataaaaa
     1921 caatattttc gcgtggaatt cccaattcgg gaattacagt ttgcgcttgt ttcaatatcg
     1981 aataatcgcc acagccggga caccaacgta tatcctgatc ggttactaaa tctttggctg
     2041 taaatccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt attgttgttg acatatcttt
     2101 ttgttttttt atttttaatt ttaattcaaa atcaggcaat catcgattct attttttcca
     2161 ttatctcaac cgaagaaaat ggaatgccct ttattttatt taatccttcg gccggtacca
     2221 aatatttact gcgcaacatg gataaaaatt gtccggtatt catttcggga accaatattt
     2281 tttcgaaacc gtataaaatt tcgcccaggt tttttggaaa tggattcatg taacgcactt
     2341 gtgcatggct aacatcatat cccttggcac gcgcctgtat caccgccatt
//
LOCUS       DAORQA010000273         2373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10135_length_2373_cov_6.714840, whole genome shotgun sequence.
ACCESSION   DAORQA010000273 DAORQA010000000
VERSION     DAORQA010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10135_length_2373_cov_6.714840"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>876
                     /locus_tag="PK736_08970"
     CDS             <1..>876
                     /locus_tag="PK736_08970"
                     /inference="COORDINATES: protein motif:HMM:NF037155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRC33557.1"
                     /translation="MASDVFIELANTITALGDGSIREAFYIDAGNYNRGLLQGKLFTV
                     VFDESGRPVNRENYVSIHTQPYYVGLKTNDSYVGLDLPLQFAYCAVDVNDKPVINLKT
                     KFEIIKRDWYTVMEKYGNGYRYNSQQRERVVYSTNVAINKNGTATYIPKLNGEYEARL
                     YTPGSSAYVSSYFYAYRSGYSENNSFETNNEGNVEIESDKDEYNTNDEVKLLFKTPFA
                     GKLIVTLEQDRVIKYYSLKTDNKSAVLNFKLANQHVPNVHIGVTLIRAQDDGSNPLIV
                     AHGYKNIIVKNPEKK"
     assembly_gap    877..910
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <911..>1208
                     /locus_tag="PK736_08975"
     CDS             <911..>1208
                     /locus_tag="PK736_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33558.1"
                     /translation="INIKTKPSTAVAVAVVDEGILQIKNYESPDPYKYFYQPRALEIQ
                     SYDIYPSLFPEVMLKKSYSGGDGFDMAKRVNPITNKRVKLVALWSGLMKSDASG"
     assembly_gap    1209..1241
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1242..2357
                     /locus_tag="PK736_08980"
     CDS             <1242..2357
                     /locus_tag="PK736_08980"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRC33559.1"
                     /translation="IPQFNGNLRIMAVAYTEDKFGAADTNMIVADPIVINSGLPRFAS
                     PGDSIIMPITLSNTTKKDLQAIVTTTVSGPFAVSTSKQNVTIKANNELRAFNKITALK
                     NIGAGKITVTVNAGSETFIEETDMTGRPPSSLQKISGSGILAAGKSVDLNVAEDLLAQ
                     ASSSKIIVSRSPVCQLGKSIEYLIQYPYGCVEQTTSAAFPQLYYNDLAREILQKGQSD
                     PNPNYNVMQAIAKLQSMQMSSGALTYWPGGDYESWFGTVYALHFLIEAKKAGFDVDKD
                     VQNRMTNFIIRKLGEKITFKYYYNSNQVKDIAPKEVAYSLYVLALAGKPQTSIMNHYK
                     SNLQLLSLDSKYLLASAYSITGNKMAFSQILTPAFSG"
BASE COUNT          776 a          436 c          466 g          628 t
ORIGIN      
        1 catggcatcc gatgttttta ttgaattggc aaataccatt actgcacttg gcgatggatc
       61 tattcgtgag gcattttata ttgatgcagg taattacaac agaggattgt tgcagggtaa
      121 attatttacc gtagtgtttg atgaatcggg aagacctgta aatcgtgaga attatgtttc
      181 gatccacact caaccatatt atgtgggatt aaaaacaaac gactcatatg taggcctcga
      241 tttgccttta caatttgcct attgtgcagt tgatgtaaac gataaacctg ttattaattt
      301 aaaaacgaaa ttcgaaataa taaaaagaga ttggtacacg gtgatggaaa aatatggcaa
      361 tggctataga tataattcac aacaacgcga gcgtgtagtt tacagcacca atgttgccat
      421 taacaaaaat ggaacggcaa catatattcc aaaattgaat ggtgaatatg aagcgcgttt
      481 gtatactccc ggttcaagtg catatgtatc ctcatacttc tatgcgtatc gcagcggcta
      541 cagcgaaaac aattctttcg aaaccaacaa cgaaggaaat gtagaaatag agtcggacaa
      601 agacgaatac aataccaatg acgaagtaaa gttgctcttc aaaactcctt ttgcaggcaa
      661 gcttatagtt acacttgaac aggacagagt gatcaaatat tattcactaa aaacagataa
      721 caagtcggct gtgcttaatt tcaaattggc aaaccaacat gtgccaaacg tgcatattgg
      781 ggtaacatta atacgcgctc aagacgatgg cagcaatccg cttattgttg cacatggtta
      841 caaaaatata attgtaaaaa atcccgagaa aaaattnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn ccatcaacat aaaaaccaaa ccaagtactg ctgtggctgt tgcagtggta
      961 gacgaaggta ttttgcaaat taagaattat gagtcgcccg acccgtacaa atatttttat
     1021 cagcctcgcg cgttagaaat acagtcgtac gatatatatc cttcactgtt tcccgaagtt
     1081 atgctaaaaa aatcgtactc gggtggtgat ggttttgata tggctaagcg agtaaatcct
     1141 attacgaaca aacgcgttaa acttgttgca ttgtggagtg gcttgatgaa atccgatgca
     1201 tcgggcaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattcctcaa tttaacggca
     1261 acttacgcat tatggctgtg gcatatactg aagataaatt tggtgcggcc gacaccaata
     1321 tgattgtggc cgatcccatt gttatcaatt cgggattgcc aaggtttgca agcccgggcg
     1381 atagtatcat aatgccaata acattaagta atactaccaa aaaagactta caggcaatag
     1441 taaccacaac tgtaagcgga ccatttgcgg tttctacatc gaaacaaaat gtgactataa
     1501 aagccaacaa cgaattacgt gcgtttaata aaatcactgc attaaaaaat attggcgctg
     1561 gtaaaataac ggtaactgta aacgcaggca gcgaaacatt tattgaagaa actgatatga
     1621 cagggaggcc tccatcatca ttgcaaaaaa taagcggaag cgggattttg gcagccggta
     1681 aaagtgttga ccttaatgtt gccgaagact tattggcaca ggcatcatca tccaaaataa
     1741 ttgtaagtcg ttcacctgtt tgtcagttgg gcaaaagcat cgaatactta atacaatatc
     1801 cgtatggatg cgtagaacaa actaccagtg cggcttttcc acaattgtat tataatgatc
     1861 tggcacgcga aattttgcaa aaaggccagt cggatcctaa tccaaattac aatgtaatgc
     1921 aggcgattgc caagttacaa agtatgcaaa tgtcatcggg tgctttgact tactggcccg
     1981 gtggcgatta cgaaagttgg ttcggaacag tgtatgcttt acatttttta attgaagcaa
     2041 agaaagccgg tttcgatgtt gacaaggatg tgcaaaaccg aatgacaaat ttcataattc
     2101 ggaaacttgg cgaaaaaatc actttcaagt attactacaa tagcaatcag gtaaaagaca
     2161 tcgcacccaa agaagttgct tactctttgt atgtgctggc attggctggc aagccacaaa
     2221 cgtcaatcat gaaccattac aaatcgaatt tgcaattgct cagtctcgat tcaaaatatc
     2281 tgcttgcctc agcatactct atcaccggaa ataaaatggc attctctcaa atactgactc
     2341 ctgcatttag tggctaagta tcgaaaactg tta
//
LOCUS       DAORQA010000274         2368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10157_length_2368_cov_7.894509, whole genome shotgun sequence.
ACCESSION   DAORQA010000274 DAORQA010000000
VERSION     DAORQA010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10157_length_2368_cov_7.894509"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..405)
                     /locus_tag="PK736_08985"
     CDS             complement(181..405)
                     /locus_tag="PK736_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33560.1"
                     /translation="MKKFAGIFLFLISIAGWYAIYKMYNRWTEVKLYGNKGSDYVSGY
                     GVGILIMAIIVALMMIIGYRMANPFSKKNN"
     gene            591..1481
                     /locus_tag="PK736_08990"
     CDS             591..1481
                     /locus_tag="PK736_08990"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRC33561.1"
                     /translation="MNLLNNILYYIIVKPLSLLPLKVLYLVSDFFYVMIYYVLPYRKK
                     VVYNNLRKSFPDYTDPQIKAVSKQYYKHLCDIIIETVKAFSLSKEELLHRFVCTSPDV
                     INQLNRTDKSIILITGHYNNWEWGGLAFPLHFTHKGYGIYTELSSNYWDIKVKAMRQH
                     INLSMIPMKDTLKCFRNNQTEKKVVAFIADQTPVSVDKCYWIKFLHQDTPVHKGAARF
                     AKMFDCVVVYGDIQKLKRGKYEVSYRVLCDNTKDIAEDDITKLHVKILEQKIIDKPQY
                     WLWTHKRWKRAPPPGTIIND"
     gene            complement(1487..1951)
                     /locus_tag="PK736_08995"
     CDS             complement(1487..1951)
                     /locus_tag="PK736_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33562.1"
                     /translation="MSQEQEQGKEQESSWFDSVKKTVGNLADKADELKDQLEDKVKDL
                     AEQAEAKAGELKKQFDESDIKEKMQEKFADLKEDVKEMADKAEAKVDELKHKAQESGI
                     MDKAEGIMDSIKHKAGELADKAESTFENLSEAAKAKLKDAKDSISGDDEKKA"
BASE COUNT          757 a          437 c          403 g          771 t
ORIGIN      
        1 attataaaaa accaccacgc catggaggct gtccaattta tcacctattt tgtgtgagcg
       61 taaaaatact tttggagcta ttgtttcttt gtgattgata aacaaaaaca caccagcaat
      121 tattacaaca gcaaaaaata aaaatttata cttacgcatt tctataaaca atcgaactca
      181 ttaattattt ttttttgaaa agggatttgc catgcggtaa ccaataatca tcatgagcgc
      241 aactataatt gccattatta atattcccac cccatagccc gaaacgtaat cgctaccttt
      301 gtttccatat agtttcactt ctgtccaacg gttatacatt ttataaattg cataccaccc
      361 tgctatgctg atgagaaaca gaaatattcc ggcaaatttt ttcactacta ataaaatgag
      421 ttattgaagt ttgcaaaata ataaattata ccgtaataac tgacgttaca taattgcatt
      481 cggtttgatg tttaaaaatg caagcagaaa atagttttac ttcaaaagtc tgtcaacttc
      541 tggtatactt gcatcataat tccaaatact taaaacggca ttcgaattta atgaacttat
      601 taaacaatat actttactac atcattgtaa agcctttgtc gttgcttcct ttgaaggtgc
      661 tctatcttgt gagcgatttt ttctatgtga tgatttacta cgtactacca taccgcaaaa
      721 aggtagttta caacaatctt cgcaaatcat ttcccgatta taccgacccg caaataaaag
      781 cggtttcgaa acagtactac aaacacttat gcgatataat tatcgaaacc gtaaaagcat
      841 tttcgttgag taaggaagaa ctgctccacc gctttgtttg cacttcgccc gatgtaatca
      901 accaattaaa tcgaactgat aaatcaatca tactaattac aggacattac aacaattggg
      961 aatggggcgg cttggctttt cctttgcatt ttacacataa gggttatggt atatatacag
     1021 aactgagcag caattattgg gatataaaag taaaggccat gcgtcaacat attaacctaa
     1081 gcatgatacc tatgaaagat actttgaaat gtttccgcaa taatcaaaca gaaaaaaaag
     1141 tggtagcttt tattgccgat caaactccgg tttcggtaga taaatgttac tggataaaat
     1201 ttttacatca ggacactcct gtgcataaag gagctgcgcg gtttgcaaaa atgtttgatt
     1261 gtgtagtagt gtatggcgat attcaaaaat tgaagcgggg taaatacgaa gtttcgtaca
     1321 gggtgttgtg tgataataca aaagacattg ccgaagatga tattacaaaa ttgcatgtaa
     1381 aaattcttga gcaaaaaata attgacaagc ctcaatattg gttatggaca cacaagaggt
     1441 ggaaacgtgc tccacctccc ggtaccatta ttaacgatta aattgcttag gcctttttct
     1501 catcatcgcc actaatactg tctttggcat cttttaattt tgccttggca gcctcgctca
     1561 gattttcgaa agtactttct gctttgtcgg ccaattcgcc cgctttatgt tttatactat
     1621 ccataatacc ttcggcttta tccattatgc ccgactcctg cgctttgtgt tttagttcat
     1681 ccacttttgc ttccgctttg tctgccattt cttttacatc ctcttttaag tcggcaaatt
     1741 tctcttgcat tttttccttg atgtcgcttt catcaaattg ctttttcaac tctccggctt
     1801 tggcttcggc ttgctcggca aggtctttta ctttatcttc aagttgatct ttcaactcat
     1861 cggctttgtc ggcaaggtta cctacagttt tttttacgct atcgaaccat gatgattcct
     1921 gttctttgcc ttgttcttgt tcttgactca tttcgttttg ttttaaataa tttatgtgcg
     1981 aagataaaaa taaattcagt aaggattaag gcaaaagtta aggagataat tcaaaagaga
     2041 aaagtgaatg gtcaaatgtg agtggtgaat tgtgaatagt gaattgtgag ttgtgaattg
     2101 ggtattgagt attgagtatt gagtatttgc aattgggagg cagaaattta aaatttagaa
     2161 ttgcttatta atgtggacga aatagaacac aattgtttgt tgggacacaa acaatggctg
     2221 gcgcaaacaa aaaacccaaa acgaagaatg caaactcttt tgtcaccaac cggactgaag
     2281 tgccgcgact aaaaacgtca aactaagaac tataaactaa gaactataaa ctaagaacta
     2341 taaactaaga actataaact aagaacta
//
LOCUS       DAORQA010000275         2368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10161_length_2368_cov_4.213143, whole genome shotgun sequence.
ACCESSION   DAORQA010000275 DAORQA010000000
VERSION     DAORQA010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10161_length_2368_cov_4.213143"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PK736_09000"
     CDS             <1..843
                     /locus_tag="PK736_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33563.1"
                     /translation="SLVPGVHFIFKPKNIKSVSKHVLTLQSFMVWSNNNFVVSNDNVA
                     QKSTNEIYPQIKYNYDNKRTLDPFDFEACLEMVNGDFTWRPKLTAAFRYYLSYGGMRK
                     AFTARIFAGITSNGTKYAQQGYASGLYMSGTNTYLDYRREYLFLGRNEYGSSNIASHQ
                     MVANEGGFKMYTATANSGKWITSLNLSARIPKLKFIGVFADVGTYYNAGNTFEGSKEV
                     VFDAGASLIIVQNAFEIYFPIKWSSDIEQFYEVNNSKKYSDKIRFQLNLHLVKPYKYL
                     SQLF"
     gene            862..1612
                     /locus_tag="PK736_09005"
                     /pseudo
     CDS             862..1612
                     /locus_tag="PK736_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
     gene            1884..>2368
                     /locus_tag="PK736_09010"
     CDS             1884..>2368
                     /locus_tag="PK736_09010"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HRC33564.1"
                     /translation="MGFKFTNKLFINIVVLGIMSIAATLRRDDFTHTQIAADNIAWLQ
                     GTWMHLSDDGRVYAQFSKDKNDVLNGMVFTLTENGDTNLTTKYKINFKRRNFLMQCID
                     YRNQASNITNYQMVHGNNNKILFEFKNETDIKLLEMRHTNNNAMVVMKTDSYSQNKSE
                     ET"
BASE COUNT          777 a          392 c          452 g          747 t
ORIGIN      
        1 tcattggtgc ctggtgtgca ttttattttt aaacctaaaa atataaaatc tgtaagcaaa
       61 catgtattga ctttgcaatc gttcatggtt tggagtaata ataattttgt agtaagtaac
      121 gataatgtag cgcaaaaaag caccaatgaa atttaccccc aaataaaata taactatgat
      181 aataaacgta ccctcgaccc attcgatttt gaagcgtgtt tagaaatggt aaacggtgat
      241 tttacctggc gaccaaaact aacagctgcg tttcgatact atttgtcgta tggtggtatg
      301 cgcaaagcat ttactgcccg aatatttgcc ggcattactt ctaacggaac taaatatgct
      361 caacagggtt atgcttcggg tttatatatg agtggaacaa acacttattt agattacagg
      421 cgcgagtatt tgtttttagg ccgaaacgaa tatggcagtt caaacattgc atcgcatcaa
      481 atggttgcaa acgaaggtgg ttttaaaatg tacaccgcaa ctgccaacag tggtaaatgg
      541 ataacatcgc tcaatttatc tgcacgcatt cctaagttga agtttatagg cgtgtttgcc
      601 gatgtaggaa cttattacaa tgcaggcaat acgttcgaag gttcgaagga agtggtgttt
      661 gatgccgggg cttcgcttat tattgtacaa aatgctttcg aaatttattt ccctataaaa
      721 tggtcgagtg atattgagca attttatgaa gtgaacaatt cgaaaaagta cagcgataaa
      781 atccggtttc aacttaattt gcatttggta aaaccttaca agtatctttc gcaattattt
      841 tagcaaaatt attttcgtct tttgaaaaaa atatatttca tttccgattt tcatcttggt
      901 gtacccgatc acgaatcgag ccttgcccgc gaaaaaaaaa atagtccgtt ggctcgatat
      961 gataaaagcc gactgcgacc aactttattt gcttggcgat gtgttcgatt tttggttcga
     1021 atataaacat gctgtgccta aaggttatgt gcggctgttg ggtaagcttg ccgagctaag
     1081 tgacagtgga atacaaatac attactttac aggcaatcac gatatgtggg ttttcgatta
     1141 tttgccacga gaaataaatt gcaaaatata tcgcgagccg gttgtactta atttagatgg
     1201 taaaaaattt tttataggtc atggcgatgg acttggcccg ggcgatacag gatataaatt
     1261 tataaaaagt gttttcgctt cacgcatttc gcagtggttg tttgctcgtt ttcatcccaa
     1321 ttttggatat tggcttgcaa atctaagtag ccgcaaaagc cgctcgatga caggcagtaa
     1381 cgatgaaatt tatcttggtg atgagaaaga aatgctggtg caattttgta aatccacact
     1441 aaaaaaggag caaatagatt tttttatttt cggtcaccgc catttgaaaa tagacatgat
     1501 gattgatgga aaagcccgct acataaacct tggcgaatgg atgcatcaaa gtaactatgc
     1561 tgtttggaat ggatcaagct tgaattttac aaattttgat gacgaaaaat agaggactaa
     1621 atcttttcaa cattattaag aatttcaaaa ctaatttgtt gataactgta acatccattg
     1681 ttaattacac atttgcattt ttcgaacact taccgtaata attcaagcat tctgcctata
     1741 tttttctata actttacgtg gtaatttcac ttgtttaccc tatataaaca ctaagatgtt
     1801 agtaaccaac atgttgtgta aaactttgaa gctgttaaca tgacgtattt tcaccattca
     1861 agattagcac tcaaaactga agaatgggat ttaaatttac caataagtta tttatcaata
     1921 ttgtagtatt gggaataatg tcgattgctg caactttaag gcgcgatgat tttactcaca
     1981 cccaaattgc tgccgataat attgcatggt tgcagggaac ctggatgcac ctttctgatg
     2041 atggacgtgt ttatgcccag ttttcgaaag acaaaaatga tgttctgaat ggaatggtgt
     2101 ttaccttaac tgaaaatggt gatacaaacc ttacaactaa atataagata aactttaaaa
     2161 gacgcaattt tttaatgcaa tgtatcgact atcgcaatca agcttcaaac attaccaact
     2221 accaaatggt acatggaaac aataataaaa ttttgtttga gtttaaaaat gaaacggata
     2281 ttaaattgtt ggaaatgcgc catacaaata acaatgcaat ggtagttatg aaaaccgata
     2341 gctactcgca aaacaaaagt gaagaaac
//
LOCUS       DAORQA010000276         2346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10271_length_2346_cov_7.337844, whole genome shotgun sequence.
ACCESSION   DAORQA010000276 DAORQA010000000
VERSION     DAORQA010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10271_length_2346_cov_7.337844"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..935
                     /locus_tag="PK736_09015"
     CDS             438..935
                     /locus_tag="PK736_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRC33565.1"
                     /translation="MLPAYKQILQQSISQKEETKKFLHKLKRTKPNDLDNNCNTLHYK
                     AFENIDCLQCANCCITTGPLLLHKDIERLSQSLSMRQAAFTNQYLRQDEDGDLVFKNM
                     PCPFLDEHSYCNVYEARPNACREYPHTQQRKIINKLDITFHNSTICPAVAKVVEGLKK
                     IYGNT"
     gene            970..1602
                     /locus_tag="PK736_09020"
     CDS             970..1602
                     /locus_tag="PK736_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33566.1"
                     /translation="MIKNFSVKIIGIAISVLCFFSNCATDEKPAATNSNATPVETQQT
                     GNAPLVTPNFGATPADVNATPNPTASAQPAPAPAPAVNTNIPNYEKFAHFICPKACKG
                     SGSEVFGGKCPVCATAYVHNENSDGHRAEMARVKTVDDAKVNMQFTDTVVSINKPSNY
                     EKTAHYSCPKACKGSGSEAPGKCPTCNSEYAHNNNSLHHEYEEKLKLKKH"
     gene            complement(1736..>2346)
                     /locus_tag="PK736_09025"
     CDS             complement(1736..>2346)
                     /locus_tag="PK736_09025"
                     /inference="COORDINATES: protein motif:HMM:NF023503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HRC33567.1"
                     /translation="TYMAAKSAAVISPVAMNIIYIGLENPVSISVPGIAPEKLSVTTT
                     GGGLTLQKVSGSNYIAKATSVTQIASVNCYAEIDGRKVLMGTQNFRTREVPTPIATVG
                     KIKGGRIATSLLLAQSGIVPLLENFDFNNVFYRITSFTFSLYGKRVQVSGLQSTSNAL
                     TQPMKDIIKKCAYNDKVIFENIKAVDPAGKTRTLPPVSFDLQ"
BASE COUNT          739 a          461 c          449 g          697 t
ORIGIN      
        1 tagtttagga gttcaatagt tcaaagttca atagtttagg agttcaatag ttcaatagtt
       61 ttttgcgaag cattcattgc cgttcggctt tagccaacgg aattattagt tcaatagttt
      121 ataaggttga gtccactccg aaaaggtatt tttgccacaa aaacacgaag gcacaaaatt
      181 tcactaaata gtaaacaact catttataat gttttgtggg atttggtgtt ttggtgaatt
      241 agtggcagtt ttccttttta gacttttcgg aggagactct aaggtttaat agttcaaaag
      301 tttagaaagt tgcgaagatt tagaaagtga aaagcgcaat aactgtaagc aatgaaaagt
      361 cctttgtgtg gttaaaatat tttgagtaca agaaactgaa aactccaaac ttcttaaact
      421 aaaaacttcc caaaaaaatg ctccctgcat acaaacaaat attacagcaa tctatttcgc
      481 aaaaggagga aacaaaaaaa tttcttcaca aattgaagcg tactaaaccc aacgacctcg
      541 ataataattg caacacattg cattacaaag cattcgaaaa tatcgattgt ttacaatgtg
      601 caaattgttg tattaccaca ggtccgctac ttttacataa ggatatcgaa cgcttgtcgc
      661 aaagtttaag tatgaggcag gcggcattta caaatcaata tttgcggcaa gatgaggatg
      721 gcgatttggt ttttaaaaat atgccgtgcc cgtttttgga tgaacatagc tattgcaatg
      781 tgtatgaagc ccggccgaat gcatgtcgcg agtatccaca cacacaacag cggaaaataa
      841 tcaacaaact cgatatcact tttcacaatt caacaatttg ccctgccgtt gcaaaagttg
      901 tggaaggctt aaaaaaaata tatggcaata catagtttat gtttactttt gaaaaaaaat
      961 aataaaacga tgataaaaaa ttttagcgtt aaaataattg gcattgcaat aagtgtgtta
     1021 tgcttttttt cgaattgtgc taccgatgaa aaacctgcgg ctaccaattc aaatgccaca
     1081 ccggttgaaa ctcaacaaac tggaaatgca ccattagtaa caccaaattt tggcgccaca
     1141 cctgccgatg ttaatgcaac accaaaccca actgccagtg ctcaacctgc accggcaccg
     1201 gctcctgctg taaataccaa tattcccaac tacgaaaagt ttgcgcattt tatttgcccc
     1261 aaagcatgca aaggtagcgg cagtgaagtg tttggtggta agtgccctgt atgtgctaca
     1321 gcatatgttc acaacgaaaa ttcggatgga catcgtgccg aaatggcgcg tgtaaagaca
     1381 gtagatgatg ccaaggttaa catgcaattt actgataccg tagtgagtat taacaaacca
     1441 tcgaactacg aaaaaacagc acactatagt tgccctaagg cctgtaaagg tagcggtagt
     1501 gaggctcccg gcaaatgccc aacttgcaat agcgaatatg cccacaacaa caattcgtta
     1561 caccacgagt acgaggagaa actcaaactg aagaaacact agattttttt gtattcattt
     1621 ggcgattgac ttcatcataa caattgctaa agctttaatc ggctcgccta cgtatacatt
     1681 caccgttgtt tatctatttt cggtgctcga ctattagttc tttcttgtag aacatttatt
     1741 gtaaatcgaa acttaccggt ggcagcgtac gggtttttcc ggccgggtca accgctttga
     1801 tgttttcgaa aataacttta tcgttataag cgcatttttt tattatatct ttcattggct
     1861 gcgtaagcgc attacttgtt gattgaaggc cactcacctg cactcttttt ccatacaaac
     1921 tgaatgtaaa gctggtaatg cgataaaata cattattgaa gtcgaaattt tcgagtaagg
     1981 gaacgatgcc actttgtgca agcaataaac tcgtggcgat tcttcctcct tttattttac
     2041 caactgttgc aatgggtgtt ggtacttcgc gtgtgcggaa attttgtgta cccatcaaca
     2101 ctttgcgtcc atcaatttcg gcatagcaat ttacacttgc gatttgtgtt acacttgttg
     2161 ctttagcaat gtaattgctg ccacttactt tttgtaatgt aagaccacct ccagtggttg
     2221 ttaccgataa tttttcaggc gcaataccag gcaccgaaat tgaaacagga ttttccagcc
     2281 caatgtaaat aatgttcatg gccacaggcg atatcacagc tgccgatttt gcagccatgt
     2341 atgttt
//
LOCUS       DAORQA010000277         2342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10293_length_2342_cov_4.087888, whole genome shotgun sequence.
ACCESSION   DAORQA010000277 DAORQA010000000
VERSION     DAORQA010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10293_length_2342_cov_4.087888"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..2314)
                     /locus_tag="PK736_09030"
     CDS             complement(128..2314)
                     /locus_tag="PK736_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRC33568.1"
                     /translation="MRKIFIIALAALFVSQVKAQHKANFDYNISKKITRTDNRGEWMS
                     VFVQGDVNTIKQTALSLQAKIKYTAGDIVAMEIQVGKIATLITTEGVQRLESRPAHNV
                     TLNDTMRVNNNINEIHQGLSPLTQPYTGKNVVMGFIDTGIDYLHPDFLDTAGKTRIAF
                     IWDHSLPANSNTPAPYTYGQEFTAADIDGSACTQVAADGHGTYVAGVGAGNGLATGTN
                     KGVAPDADIIMVKYDFSSSAPGRIADAVAYIFAKAALMGKPCVINASLGDYYGSHDGR
                     DLEAQAIKNLITTQPGQLMVGAIGNFGNRPYHLGYTATSDTNFTWFKFDNALGSFIPG
                     LGVYIQAWGDSADMKNIQFSIGADANKATYSYVGNTAYKFIPAILNQSFYDTLKNAQG
                     QRVGIIERFLSKQSNTYSMEFYIFPDSTSYLWRLNATGNGRFDLWDLLYTNYQTGMEN
                     SGLPSSAINPEIVNYVLPDTLMTNVSSFACLDEVITVGDYLNRNSHRNCQDSLVSLVG
                     FSPKGSIISNSSHGPTRDNRTKPEVCASGSYNMAAFPLAYFAANATSNKNLKGCYHYG
                     SGGSSFAAPVVAGIGALFLEKNPQLTAAQFRQYIICAAKTDTSTGLALPNNTWGYGKV
                     NGFNSLLMCGVGINENIAANYYVTVFPNPFSNFTQIKIGGLNENDFMQLQVMDVTGKI
                     LINQSRKAINTNELFTVSKNNLSAGIYFYKVVIDGGKIFTGKLVVE"
BASE COUNT          669 a          510 c          472 g          691 t
ORIGIN      
        1 aacaaaacta agaatgccga cacttcggtc ggcatcccga ccaaagtgtc gggactagga
       61 caatccactc cgcaaacgaa actaaaaatg ctggcatccc gaccgaagtg tcggggctaa
      121 gaaaaaatca ctccaccacc aacttcccgg taaagatttt tccgccatca atcaccactt
      181 tataaaaata aataccggcc gaaagattat ttttcgacac tgtaaatagc tcattggtat
      241 tgattgcttt acgcgattga ttgattaata tttttccggt tacatccatt acttgcagtt
      301 gcataaaatc gttttcgttt aaaccaccaa tttttatttg tgtaaaattt gaaaatggat
      361 ttggaaatac ggttacataa taattggcag caatgttttc attaatgcca acaccacaca
      421 taagcaaact attaaagcca tttaccttac catatcccca cgtgttgttc ggtaatgcta
      481 agccggtact tgtatcggtt ttagcagcgc aaataatgta ttgcctgaac tgtgcagcgg
      541 tgagttgcgg atttttttct aagaacaatg caccaatgcc ggcaacaaca ggtgcagcaa
      601 acgatgaacc acccgaacca taatgataac atccctttaa gtttttatta ctggttgcat
      661 tggctgcaaa gtaagcaagt ggaaatgctg ccatattata cgatcccgaa gcacacactt
      721 cgggcttagt gcgattgtcg cgtgtggggc catggctact gttgcttatg atactaccct
      781 ttggcgaaaa gcctacaaga cttaccaacg aatcctgaca attgcggtga ctgttgcgat
      841 tgagataatc gcccacggta atcacttcat cgaggcatgc aaaactgctt acgtttgtca
      901 tcagtgtatc gggcaaaaca taatttacaa tttcgggatt aattgctgaa gaaggtaatc
      961 cactgttttc cattcctgtt tggtaatttg tataaagcaa atcccacaaa tcgaaacggc
     1021 cgttgcccgt agcattcaat cgccataagt aagaagtgga atcgggaaaa atataaaact
     1081 ccatcgagta tgtgttgctt tgtttcgaca aaaatctttc aataattcca acccgttggc
     1141 cttgcgcatt ttttagtgta tcgtaaaaag actgatttaa aatagcagga ataaatttgt
     1201 aggctgtatt acctacataa gaataagttg ctttattagc atcggcacca atggagaatt
     1261 gaatattttt catgtcggca ctatcgcccc atgcctgaat gtaaacacca agccctggaa
     1321 tgaaacttcc caatgcatta tcaaatttaa accaggtaaa atttgtatcg ctcgttgccg
     1381 tgtatcctaa atgataaggg cgattaccaa aatttccaat tgcacctacc atcaattgtc
     1441 cgggctgcgt ggtgataaga ttctttatcg cttgtgcttc gaggtcgcgg ccatcgtgcg
     1501 aaccatagta atcgccaaga cttgcattaa taacacaagg cttgcccatg agcgcggctt
     1561 ttgcaaatat gtatgcaact gcatcggcaa tgcggccagg ggcactacta ctaaaatcat
     1621 atttcaccat aattatatcg gcatcgggtg ccacgccttt gttggtgccc gtggcaaggc
     1681 catttcctgc accaacgccc gctacataag ttccatgccc atcggctgca acttgagtac
     1741 aagcactgcc atcaatatca gcagctgtaa attcttgtcc gtacgtgtat ggggcaggtg
     1801 tattgctatt tgctggcaaa gagtggtccc aaataaatgc gatgcgagtt ttgccggcag
     1861 tgtccaaaaa atcaggatgc aaataatcga tacccgtatc aataaaaccc atcacaacat
     1921 tttttccggt atagggttgc gtaagcgggc tcaaaccttg atgtatttcg tttatattat
     1981 tgttcacacg catggtgtcg ttcaatgtca cattgtgggc ggggcggctt tcaaggcgtt
     2041 gcaccccttc agtggttatt agtgttgcaa tttttccaac ttgtatttcc atggcaacta
     2101 tgtctcctgc agtgtatttt atttttgcct gaagcgatag tgctgtctgc ttaatagtat
     2161 tcacatcgcc ttgcacaaaa acactcatcc attcgccacg attgtcggtg cgggtaattt
     2221 ttttgctgat gttataatcg aaatttgctt tgtgctgagc tttcacttgc gaaacaaata
     2281 gtgcagctaa agcgatgata aaaatttttc tcattgaatt ttgtttttat gtgatgtaaa
     2341 ga
//
LOCUS       DAORQA010000278         2340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10299_length_2340_cov_6.892779, whole genome shotgun sequence.
ACCESSION   DAORQA010000278 DAORQA010000000
VERSION     DAORQA010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10299_length_2340_cov_6.892779"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..735)
                     /gene="kdsB"
                     /locus_tag="PK736_09035"
     CDS             complement(10..735)
                     /gene="kdsB"
                     /locus_tag="PK736_09035"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRC33569.1"
                     /translation="MNIIGIIPARYASTRFPGKPLVDINGKTMIQRVYEQALGCELLN
                     DVIVATDDERIANHVKQFGGKVEMTSPNHQSGTDRCCEVIQLLPYTPDAIINIQGDEP
                     FIQPEQISMLAQLMSNPNVEIGTLIKEITDATEITNENVVKVVADNFGKALYFSRCAI
                     PFQRNKSQNVPSYYKHIGMYGYKREVLQKIAGLKPSPAEISESLEQLRWLQNGYIINT
                     AITNFETFAVDSPDDLKKFNLSN"
     gene            complement(748..1386)
                     /locus_tag="PK736_09040"
     CDS             complement(748..1386)
                     /locus_tag="PK736_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRC33570.1"
                     /translation="MEEPRTVVVYMEQTPNPAALKFVTNAALLVQGSAEFTNKQDALA
                     SPLAYSLFDFTGVRSVFIAENFVTVTKTEEIEWWDIQNIVREYIRSYLVSGDDIFDKE
                     LMAQLQSKPRKTEIHAAAVPVEKPTPQHYSDAENNAISEKIMATLDEYIKPAVAQDGG
                     AIDFKSFEDGIVTLVLKGSCSGCPSSTITLKHGIERVLKSMIPEVSEVIAEQ"
     gene            complement(1397..>2340)
                     /gene="lhgO"
                     /locus_tag="PK736_09045"
     CDS             complement(1397..>2340)
                     /gene="lhgO"
                     /locus_tag="PK736_09045"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HRC33571.1"
                     /translation="IIVATQKSEIEQLHKVYNNGLANGVEGIERISGLQIKDIEPHCH
                     GIEGLWVPCTGIIDFAQVARTYVSLLQNKFADCNIAMGEEVKDFEKQDTYTTIVTNKS
                     QYKAKYIINCTGLHCDRVAKKDGVNPEMKIVGFRGDYYELTPEAKYKVKNLIYPVPNP
                     EFPFLGVHFTRMVNGETECGPNAVFTFKREGYGKTDFNLRDTFEALTYGGTWKLFFKH
                     WRFGLDEYRRAFSKKLFLSRLQLLVPSLTMDDIKPGRAGVRAMALDSGGNMIDDFKIV
                     NSANALHVLNAPSPAATASLAIGNEIRMMAEKHFQLN"
BASE COUNT          604 a          491 c          441 g          804 t
ORIGIN      
        1 gatttacaat tagttgctta aattaaattt tttaagatca tcaggactat caacagcaaa
       61 ggtttcgaaa ttggtgatgg cagtgttaat aatatagcca ttttgtaacc accgaagttg
      121 ctccaacgac tccgaaattt cggccggaga tggttttagt cctgcaattt tttgaagtac
      181 ttccctctta tatccataca ttcctatatg cttataatag gaaggtacgt tctgcgattt
      241 gtttctttga aatggtatgg cacaccggct gaaataaaga gctttgccaa aattatctgc
      301 caccactttt actacattct cattggtaat ttctgtggca tcggttattt cttttatcaa
      361 tgtgcctatt tctacattcg ggttactcat taattgtgca agcatcgata tttgttcggg
      421 ctgaataaat ggttcatcac cttgtatatt aataattgca tcgggcgtat atggtaatag
      481 ttgtatcact tcgcagcagc ggtcggtgcc actttggtga ttgggcgatg tcatttctac
      541 tttgccgcca aattgtttta cgtgattcgc aatacgttca tcatccgtgg caactattac
      601 atcattaagt aattcgcacc cgagcgcctg ctcatataca cgctgtatca tcgttttgcc
      661 gtttatatca actaacggtt tgccgggaaa ccgggttgaa gcatatcgag caggaattat
      721 tccaatgata ttcattcgtt ggtaatatta ttgttcggca attacttcgc tcacttcggg
      781 tatcatactt ttcagcactc tttctatacc atgtttcaat gttatggttg acgaagggca
      841 accactgcac gaccctttaa gcacaagtgt tactattcca tcttcgaacg atttgaagtc
      901 gatagcccca ccatcctgtg ccactgcagg ttttatatat tcatcaagtg tggccattat
      961 tttttcggat atagcattgt tctccgcgtc cgaataatgt tgtggagttg gtttttcgac
     1021 cggtacggct gccgcatgaa tttctgtttt acgtggtttc gattgtaatt gtgccatcaa
     1081 ttctttatcg aaaatatcat cgccacttac aaggtaactg cgtatatatt cgcgtacaat
     1141 attttgaata tcccaccatt ctatttcttc ggtcttggtt actgtgacaa aattttcggc
     1201 aataaaaaca ctgcgtacac ctgtaaagtc gaaaaggcta taggctaatg gacttgccaa
     1261 tgcatcttgt ttattggtaa attcggcact gccttgaaca agcaatgcag cgttggttac
     1321 aaatttcagt gcagccggat ttggggtttg ttccatataa accaccactg tgcgaggttc
     1381 ctccattttt ttatttttaa ttaagttgaa aatgtttttc ggccatcatt cttatttcgt
     1441 ttcctattgc cagcgaggcc gtagccgcag gcgaaggtgc atttaataca tgcagtgcat
     1501 tggcactatt tactatttta aaatcgtcaa tcatattgcc accactatcg agtgccatag
     1561 cacgcacgcc agcgcggcca ggttttatat cgtccatagt aagcgagggt acaagtaact
     1621 gtaaccgact gagaaacaat ttttttgaaa atgccctacg gtattcgtca agtccaaatc
     1681 gccagtgttt aaaaaataat ttccatgttc cgccataggt caatgcttca aaagtatcgc
     1741 gaagattgaa atctgtttta ccatatccct cacgtttaaa tgtaaatacc gcattgggac
     1801 cacattctgt ttcgccattt accattcgtg taaagtgtac acctaaaaat ggaaattcag
     1861 gattgggaac agggtaaatg agatttttca ctttgtactt tgcttccggt gtaagctcat
     1921 aataatcgcc acggaaacct acaattttca tttcagggtt tacgccatct ttttttgcca
     1981 cacggtcgca atgcaagcct gtgcaattaa taatgtattt ggctttgtat tgcgatttgt
     2041 ttgtaactat agttgtatac gtatcttgtt tttcaaaatc ctttacttct tcgcccatag
     2101 caatgttgca gtcggcaaat ttattttgca ataatgatac atacgtgcgt gccacttgtg
     2161 caaaatctat gatgcctgtg cacggcaccc ataagccttc tattccatgg caatgtggtt
     2221 caatgtcttt tatttgcaat cccgatatac gttctatccc ttctacgcca tttgccagcc
     2281 cattgttata cactttgtgc aattgttcaa tttccgattt ttgagtggca actattattt
//
LOCUS       DAORQA010000279         2335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10328_length_2335_cov_4.178070, whole genome shotgun sequence.
ACCESSION   DAORQA010000279 DAORQA010000000
VERSION     DAORQA010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10328_length_2335_cov_4.178070"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PK736_09050"
     CDS             complement(<1..217)
                     /locus_tag="PK736_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRC33572.1"
                     /translation="MKRAYAYMHIAIFLWGFTGILGRAISLDYGQLVWYRMLISALSL
                     GLYIWYKKERVAPPKNEIVKMLPTGSIV"
     gene            complement(241..825)
                     /locus_tag="PK736_09055"
     CDS             complement(241..825)
                     /locus_tag="PK736_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HRC33573.1"
                     /translation="MLLNEKLKPYKIILGSQSPRRSELLHLLGINFEVKTQNAEEDFP
                     SNIDAKDVPQFLAQKKAAAYINELNNNTLVITADTIVYLNGEIINKPTDRNDAINILQ
                     KLSNNRHTVYSGVCITSAHKTISFTVASNVYFKKLTFEEIEYYIDNFKPYDKAGAYGV
                     QDWIGLVGIEKIDGSFHNIMGLPVKELYEQLMLF"
     gene            complement(832..1749)
                     /locus_tag="PK736_09060"
     CDS             complement(832..1749)
                     /locus_tag="PK736_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HRC33574.1"
                     /translation="MAYLKIIRFGNLLMAALTQWLVQLMLINPFLQGYNLPVTQWWIV
                     ACCIVATFLLGAGGNIINDIVDYNIDMINHPDSVILNKSISVNAANKLYLIVTGMGIF
                     IGSIPCVIYKYYGLILILGFMAALMWYYSYKFKSIMLFGNIVVAFVISFSILLPMFYN
                     LDTFTIKPLVQIITYYTLFSFIIVLAREIIKCMEDMHGDNEYGIRTLPIVAGINTSKT
                     IVGILFLLITVGIGYFQYIQMQGEDFFNRNCFSSLYLLLFVQLPLIYVCFTIIKAQTP
                     KQYNRLSTITKFVMLSGIMSLVVFYYLMN"
     gene            complement(1759..2247)
                     /locus_tag="PK736_09065"
     CDS             complement(1759..2247)
                     /locus_tag="PK736_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HRC33575.1"
                     /translation="MKINFKEKLQDIKAFFFDVDGVLTNGQLVIMPGGELLRSMNIKD
                     GYAMQLAVKKNYVVAIISGGKSEGVPHRLSNLVITEIHMQVKDKMEVYNLTDHNILMM
                     GDDMPDIDLLTTAGVATCPADACQQVKQCCIYQSQIKGGEGCVRDIIEQTLTLHGNWN
                     EA"
BASE COUNT          735 a          438 c          386 g          776 t
ORIGIN      
        1 ctacaataga acctgttgga agcattttta ctatttcgtt ttttggtggt gctacccttt
       61 cttttttata ccaaatatac aaacccaaac tgagcgcact tataagcatt cgataccaaa
      121 ccagctgtcc ataatcgagc gaaatagcac gaccaagtat accggtaaat ccccaaagga
      181 aaatagcaat gtgcatgtat gcataggcac gcttcattta tttattctaa aatatttttt
      241 ttaaaaaagc atgagttgct catacaattc cttcaccggc agtcccataa tattgtgaaa
      301 cgagccatca attttttcta ttccaactaa tccaatccag tcttgcacgc cataggcacc
      361 ggctttatca taaggcttaa aattgtcgat gtaatattca atttcttcga aggtcaattt
      421 tttgaaatac acattgctgg ccacagtaaa tgaaatagtt ttgtgtgcac tggtaatgca
      481 aacgccactg tacactgtat gtctgttatt actaagcttt tgcaaaatat ttatggcatc
      541 gttgcggtcg gtgggtttgt ttattatttc gccatttaaa tacacaattg tgtcggccgt
      601 aattactaat gtgttattgt taagttcatt gatgtatgct gcggcttttt tttgtgctaa
      661 aaattgggga acatctttag catcaatgtt cgaaggaaaa tcttcttctg cattttgtgt
      721 tttcacttca aaatttattc ccagcaaatg gagcaactca cttcttcgtg gcgattgcga
      781 acctaaaatt attttgtatg gttttaattt ttcgtttaac aacatattgt attaattcat
      841 aaggtaataa aaaaccacca acgacattat tcccgaaagc attacaaatt ttgtaatagt
      901 tgaaagtctg ttatactgtt ttggtgtttg tgcttttatt attgtaaagc aaacatatat
      961 taagggtaac tgcacaaata aaagcaagta tagtgatgaa aaacaattgc ggttaaaaaa
     1021 atcttcgccc tgcatttgaa tatattgaaa atagcctatg ccaactgtta tcaataagaa
     1081 taagatgcca acaatagttt ttgaagtatt tatgcctgca acaataggca aagtacgtat
     1141 gccatattca ttgtcgccat gcatgtcttc catgcatttt attatttcgc gtgccagcac
     1201 aataatgaac gagaatagcg tgtaataagt tataatttgt acaagaggtt ttatggtaaa
     1261 tgtatcgagg ttgtaaaaca ttggcaataa aatgctgaaa gagattacaa acgcaacaac
     1321 aatatttcca aataacatta tgcttttaaa cttataggaa taataccaca ttaatgcagc
     1381 cataaacccc aggataagaa tgaggccgta gtacttataa attacgcaag gaatactgcc
     1441 aataaaaatt cccattccgg taacaataag atacaatttg ttagctgcat taaccgaaat
     1501 agatttgttc aatatcacac tatcgggatg gtttatcata tcgatgttat aatctacaat
     1561 atcgttgatg atattgccac cggctccaag caaaaatgtg gcaacaatac aacaagcaac
     1621 aatccaccat tgtgtaacag gtaaattgta cccttgcaaa aatggattga ttaacatcaa
     1681 ctgcaccagc cattgggtaa gtgctgccat taataaattg ccgaatcgta taatttttaa
     1741 atatgccata taaaattttt atgcttcgtt ccagttgcca tgcaaggtga gtgtttgttc
     1801 tattatatcg cgcacacagc cttcaccgcc tttaatttgc gactggtaaa tacaacattg
     1861 ctttacctgt tggcaagcat cggcaggaca tgtggccact cctgcagttg ttaataaatc
     1921 tatatctggc atatcgtcac ccatcatcaa tatgttgtgg tcggtgagat tgtaaacttc
     1981 cattttatct tttacctgca tgtgtatttc ggtaatgaca aggttagata atcgatgtgg
     2041 cacaccttcc gatttccccc ccgaaataat ggcaacaaca taatttttct ttacagcaag
     2101 ctgcattgcg tagccatctt ttatattcat gctgcgcaat aattcacctc ccggcattat
     2161 cactaattgc ccgtttgtaa gcactccatc cacatcaaaa aagaatgctt ttatatcctg
     2221 aagtttttct ttgaaattta ttttcataac ggagaggcgt ctaaaatcta tttaaatttt
     2281 gtgtttttat tcgttacata aatatcatcg cttatgattt tgtaaagtgc tttgc
//
LOCUS       DAORQA010000280         2327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10371_length_2327_cov_4.180898, whole genome shotgun sequence.
ACCESSION   DAORQA010000280 DAORQA010000000
VERSION     DAORQA010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10371_length_2327_cov_4.180898"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2327)
                     /locus_tag="PK736_09070"
     CDS             complement(<1..>2327)
                     /locus_tag="PK736_09070"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRC33576.1"
                     /translation="LNPARIESEGFAVVVEGTNHKVLNNNITTSNIGVQEQGGAHPNS
                     GYVANNAGDGNQSDNFSANYFGRGNAPMACGNIISGNTFSGNTTNQRTSTGSGSFGLV
                     TNTNTGKLFCSIQAAIDDAQTLNGHIITVGAGTYNEQVLVNKEVTIKNATNVRPVINF
                     TGTPALVSGAKTLFEVTAPNVTIDSLEFSVDVTNLGSAILASSATFNNLTVTDNSINP
                     YRSAVGTVSFGLRNAISVNVSPRVNSNNPTMLIQNNTITYNVGVDLTPGTADDAGFRS
                     GVATDEGGGTITLNTITTISHDVLVRFGGNPAGMSITSNNFNGGGVELSDQNAACGPM
                     VVSNNTFTGAGAPNTAVLRIKNNYDGITHTISSNTFTNHEWAISSENMKAVTFNANTF
                     NTASPTAHALVINTKSISTNSAAIVQIAIAATITNNNFNGTGNAITFLNHDSDNDTYG
                     TISMGTAGNENNFAATLSTFVVFDSQTGVSSGSVFPNYTATIGAGAGALTTMACWDVN
                     LNAQNNKYDVGAGLQLPIAMSFAGRTTLETKLNHKPDNSCLGSLLYYLPVHNLNQNTY
                     YLTIQEGVNAAVAGEVLECDAWTFNERVTVDKSLTIQGVSKVNTIVSGTGLAGFGSGF
                     YINNGVTNVTIKDLTIQNFVGNSPNGFAGVYAVGGNNNLTVSNTIIKDNVGGSGIYAN
                     GPVNVVLLDSNTISGHTNVAGAARGIVIWNGLKSNITITNNEVYNNNCCGIELQDGTA
                     SGVNIYNNNVHDNSDNGLGLSGMNDATGANLVRKK"
BASE COUNT          605 a          503 c          504 g          715 t
ORIGIN      
        1 gttttttacg tacaagattg gctcccgttg cgtcattcat gccgctaagg ccgagtccgt
       61 tgtcgctatt gtcatgtaca ttgttattat aaatgttaac acccgaagca gtaccatcct
      121 gcaactcgat accgcaacag ttgttgttat acacttcgtt attagtaatg gtgatattgg
      181 atttcaaccc attccaaatt acaattccac gggcagcacc cgcaacattt gtatggcccg
      241 aaatggtatt gctatctaaa agcacaacat tgacaggtcc attggcataa attccgctgc
      301 cgcctacatt atctttgata atggtattgg aaactgtaag gttattgttg cctccaacag
      361 cataaacacc ggcaaagcca ttggggctat tgcctacaaa gttttgaatg gtaaggtctt
      421 ttatagtaac attggtaaca ccattattaa tatagaaacc ggagccaaag cctgccaatc
      481 ctgttcctga aacaatggtg tttactttgc ttactccttg tatagtaaga gatttgtcaa
      541 cagtaactct ttcgttaaaa gtccatgcat cgcactccag tacttcaccg gcaacggcag
      601 catttacacc ttcttgtatg gttaggtaat aagtgttttg atttaaattg tgtaccggca
      661 aataatataa taacgaaccc aggcaagaat tgtcgggctt gtggttcagt ttagtttcta
      721 aagtagtgcg accggcaaag ctcatcgcta taggcaattg taagccggca cccacatcat
      781 acttattgtt ttgggcattg aggtttacat cccagcaagc catagtagta agtgcacctg
      841 ctcctgctcc aatggttgcg gtgtaatttg gaaacaccga cccgcttgat acacctgttt
      901 gtgaatcgaa cactacgaac gtagagagtg tggcagcaaa attattttcg ttgccggcag
      961 tacccattga aatggtacca tacgtatcgt tatcactatc gtggtttaag aacgtaatgg
     1021 cgttgcctgt accgttaaaa ttattatttg ttatggttgc agcaattgca atttgcacaa
     1081 tagcggctga gttggtgctg atagattttg tatttataac caatgcatgt gctgtaggcg
     1141 aagctgtatt aaatgtgtta gcgttgaaag tcacagcctt catgttttcg gaacttattg
     1201 cccactcatg attggtaaat gtgttggatg aaatggtatg tgtgatacca tcgtaattgt
     1261 ttttaatgcg caatacagca gtgttgggtg ctcctgctcc ggtaaatgtg ttgtttgaaa
     1321 ctaccattgg gccacaggca gcattctggt cgctcaattc tacccctccg ccattaaaat
     1381 tatttgatgt tatactcata cctgcagggt taccgccaaa ccgcactaat acatcgtgac
     1441 tgatagttgt tattgtattt aatgtgatgg ttcctccgcc ttcgtctgtt gctacacccg
     1501 agcggaagcc ggcatcatcg gcagtacccg gagtcaaatc cactcctaca ttataagtaa
     1561 tggtgttatt ctgaatcaac attgttgggt tattgctgtt tacacgtggc gatacgttta
     1621 cgctaatggc attgcgcaag ccaaacgaaa cagtgccaac agctgaacgg tatggattga
     1681 tgctgttatc ggtaacggta agattgttga atgtggccga gctggcaaga atggcgctac
     1741 caaggttggt aacgtctaca ctaaactcaa gactatcaat agttacattg ggagccgtaa
     1801 cttcgaataa tgtttttgcg ccacttacca atgccggtgt gccggtaaag tttattacag
     1861 ggcgcacatt ggtggcattt tttatcgtca cttctttatt tactaatact tgctcgttat
     1921 atgtacccgc acctaccgta ataatatggc cgttcaaagt ttgggcatca tctattgccg
     1981 cttgtatgga gcagaacaat tttccggtat tggtattggt tactaatcca aacgaaccac
     2041 tgccggtaga agtacgctga ttggtggtgt tgccactaaa tgtattgccg ctaataatgt
     2101 taccgcaagc catcggggca ttaccacgac caaaataatt cgctgaaaag ttatcgctct
     2161 gatttccatc gcccgcatta ttggccacat atcccgaatt gggatgtgcc ccgccttgtt
     2221 cctgtactcc tatattgctg gtggtgatat tattgttgag cactttgtgg ttggtgcctt
     2281 ctaccacaac cgcaaacccc tcgctttcaa tacgtgcagg attgagg
//
LOCUS       DAORQA010000281         2321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10398_length_2321_cov_5.367167, whole genome shotgun sequence.
ACCESSION   DAORQA010000281 DAORQA010000000
VERSION     DAORQA010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10398_length_2321_cov_5.367167"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2112)
                     /locus_tag="PK736_09075"
     CDS             complement(<1..2112)
                     /locus_tag="PK736_09075"
                     /inference="COORDINATES: protein motif:HMM:NF026223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRC33577.1"
                     /translation="MANSYQNIFIDNVEIDEYPTASTLISATIFNQSTLKVGVNSVNQ
                     DIMGIRIYQKGSLGNLYLDTILFNNLGCTNFSQDVLQAKVFYTGSDSIFSTTTQFGAI
                     FNNPLATTMKFFGAGTCNTASSLKLLNGYNYLWIAYDIKPTAFVGHLVDAQFEGIKTN
                     CSPAVQGTAGTLPGSREVGASYAIPLWVYGTNNGVPGISYARRTYIGRVHIDGLNTTL
                     DNCFHDPVASPPLGYANTQTMEYAPHPNSYTYFAPVDKGVGSKKRRSVDLLRNSNYSL
                     LISSGTWIGPNLTGLNMETNNIGAWIDFNGDGDFLDTYGTISEQLGLFYNMLQGDTSF
                     NVLPSGVGYVTFPLNIPDTGIAPNGCPGCPKTKAAYVRLRISAKCSSGNPVLHPFNAV
                     PTYFHGETEDYDVRLLADVCPDSSLHICKWIAGSATDPTNWHLIENWCPRIPTIVDEA
                     WVQPNIAGNYPVIHTDSVAVCSSLKIFAPCTVTADAPDPHLVKSKASLTMAKDVMLGV
                     TPNTNGRLIVNSSYQDTVRIPNVTQVNYTLQSATNTPFAGHRTDCRYQILLSASQLTS
                     LYGLAPGDKIESIIFPIKKNSTGSYSNFRIRYYLINPASFSFVTTVTNGQEMLVNSAG
                     TTNPLGLVPVTNGPFNYFNSVSFSTTGTSTTWTDNVFPNQFGDLIWDGVKDIIFDISF
                     NNAGGTYASVNSSGFPDYVPLI"
BASE COUNT          725 a          441 c          463 g          692 t
ORIGIN      
        1 tattaaaggt acataatctg gaaagccgct agaatttacc gaagcatatg tccctccggc
       61 attgttaaag cttatatcga aaataatgtc ctttacccca tcccaaatta aatcgccaaa
      121 ctgatttgga aaaacattat cggtccaggt agtagatgta ccggttgtgg agaatgagac
      181 agaattgaaa taattgaatg ggccgttagt aactggcact aaacccaatg gatttgttgt
      241 gcctgctgag ttaactaaca tttcctgacc attggttaca gtagtcacaa aactgaatga
      301 tgccggatta attaagtaat accttattct aaagttacta taactaccag tactattctt
      361 cttgattgga aatattatgc tttctatttt atcgccaggt gctagtccgt ataacgaagt
      421 taattgtgat gcgcttagca aaatctgata gcggcaatcg gtacgatgcc ctgcaaatgg
      481 tgtattggtg gcactttgta aggtatagtt tacttgtgta acatttggta ttcgcaccgt
      541 atcttgataa ctactgttta ctattagtcg gccatttgtg tttggcgtta ctcctaacat
      601 gacatccttg gccattgtaa ggcttgcttt acttttaact aaatgtggat caggggcatc
      661 ggcagtaact gtacaaggag caaatatttt caagctacta caaactgcta ctgagtcggt
      721 gtgaattacc ggatagttac cagcaatatt tggttgtacc caagcctcat caacgatggt
      781 aggaatgcgg ggacaccaat tttcgatgag atgccagttg gtcggatctg tggcagaccc
      841 ggctatccat ttacaaatat gcaacgatga atcgggacaa acatcggcca ataaacggac
      901 atcataatct tcggtttcgc catgaaaata tgttggaaca gcgttgaaag gatgtaatac
      961 tgggttacca gatgagcatt ttgcacttat tcttagacga acataagctg cttttgtttt
     1021 agggcaacca gggcaaccat ttggagcaat acctgtatca ggaatattta atggaaaggt
     1081 tacatagccc acacccgatg gcaaaacatt aaatgatgta tcgccctgaa gcatattata
     1141 aaacaaacca agctgctctg atattgtacc atacgtatcg agaaaatcgc catcaccatt
     1201 aaaatctatc cacgctccaa tattattggt ttccatgttt agaccggtta aatttggacc
     1261 tatccaagtg cccgatgaaa taagaagtga ataattactg ttgcgcaaca aatctactga
     1321 gcgcctcttt tttgagccca cacctttatc aacaggagca aaataagtgt agctatttgg
     1381 atgtggtgca tattccattg tttgcgtatt ggcatatcct agtggaggag aagcaacagg
     1441 atcatgaaaa cagttatcca aagtagtatt taacccatca atatgaacac ggcctatgta
     1501 tgtccttctt gcatatgata taccaggtac cccattattg gtcccgtaaa cccatagtgg
     1561 aatagcgtag ctggcaccta cttcgcggct acctggcaat gttccggcag tgccttgcac
     1621 ggcaggactg caattggttt taatgccttc aaattgagca tccactaaat gacctacaaa
     1681 agctgttggc tttatatcat aagctatcca cagataatta tagccgttaa gcaatttcaa
     1741 ggaggatgca gtgttgcaag ttcctgcgcc aaaaaatttc attgttgtgg caagaggatt
     1801 attaaaaata gccccaaact gagtggtggt tgaaaaaatt gaatcgcttc cagtataaaa
     1861 tacttttgcc tgcaatacat cctgcgaaaa attggtgcat cccaaattgt tgaagagtat
     1921 agtatcgaga tataaattgc ccaaagaacc tttttggtaa atacgaattc ccattatatc
     1981 ttgatttaca gaattaacac caactttaag cgtgctttga ttgaatatgg ttgccgagat
     2041 taaagtggag gccgtagggt attcatctat ttctacatta tcgataaaaa tattttggta
     2101 tgaattagcc aatttaaaaa aacgcgaaca aaatattgat gcgcgtttta tataatcaag
     2161 taaagattgc tgtatgcctc tcaaaaaaaa ccttaaagtt gttggaatga ttgaaatcac
     2221 aaaattggaa tggcttatca gtttgctatc agccatgtgt ttgaaagaag aataaatatc
     2281 acaataataa gttggcgcat ttagttcatc cactatctga t
//
LOCUS       DAORQA010000282         2314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10429_length_2314_cov_8.892873, whole genome shotgun sequence.
ACCESSION   DAORQA010000282 DAORQA010000000
VERSION     DAORQA010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10429_length_2314_cov_8.892873"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PK736_09080"
     CDS             <1..672
                     /locus_tag="PK736_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC33578.1"
                     /translation="VQNVCKKNNAVFIVDEMITGFRWHLQGAQTYFGINPDITLFGKG
                     MANGFSLSCIAGKKEIMNLGGIHQEGMERVFLLSTTHGAEMPALAAFLETVKLYKEEN
                     IIHHLWQYGDKLIGIFENAIAENKLEEYFKIEGGCVALNFVTLNIEKKADMKFRTLFA
                     QEMIKNNILIPCIAQSFAHSDTELQLTSDALHKSLKTYKQAIENGIDNYLVGPAIKPV
                     FRKYN"
     gene            677..1411
                     /locus_tag="PK736_09085"
     CDS             677..1411
                     /locus_tag="PK736_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC33579.1"
                     /translation="MNLSNNNIIVTGSEGLLGKAIIACITQNGGNAICIDIADSSANE
                     NYFKCNVTDEAEVNSVFDTIINKYEKIDGLVNCAYPRTSDWGKYFDNESLESWNKNIE
                     LQTSNVFLTIKKLAPHFVKNKNGSVVNIASIYGVVGNNFSLYENTTITSPSAYTLIKG
                     GIINFTKYLASFYGKHNIRFNCVSPGGIYNNQNEQFVKNYNSMVPLQRMGTPADIAPS
                     VIFLLSPATAYITGHNLIVDGGWTII"
     gene            1424..>2314
                     /locus_tag="PK736_09090"
     CDS             1424..>2314
                     /locus_tag="PK736_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC33580.1"
                     /translation="MKENNNKIKVVVTGADGFIGSHLTEHLIDCGYNVTAMVLYNSFG
                     YNGWLENLAPEKLSQIKIVSGDVRDYHSVLQATRNADIIFHLAALIAIPYSYTAPDSY
                     VDTNIKGTLNIVQAAREHKTSRVIVTSTSEVYGTAKYVPIDEVHPLQGQSPYSASKIG
                     ADKIAESFYRSFQLPLTIVRPFNTYGPRQSARAIIPTIISQLLNNETEIKIGNITPTR
                     DFVFVKDTATGFEKIANANDAIGEEINIATQQEISIGNLAQMLITKINPEAKIITDEQ
                     RLRPNNSEVERLLGSAVKLLK"
BASE COUNT          819 a          423 c          423 g          649 t
ORIGIN      
        1 gttcaaaatg tatgcaaaaa aaacaacgca gtttttattg tagatgaaat gataacagga
       61 tttaggtggc atttgcaagg tgctcaaacc tactttggta taaatcccga catcaccctt
      121 tttggcaaag gcatggcaaa tggtttttct ttatcgtgca ttgccggcaa aaaagaaatt
      181 atgaatcttg gtggaataca tcaggaagga atggagcgtg tatttttact atcgaccaca
      241 catggtgccg aaatgcctgc attggcagcc tttctcgaaa ctgtaaaact atataaagaa
      301 gaaaatatta ttcatcatct atggcaatat ggcgataaac ttattggtat tttcgaaaac
      361 gctattgccg aaaataaatt agaagaatat tttaaaatcg aaggtggttg tgtagcatta
      421 aattttgtta cactaaatat tgaaaaaaag gcggacatga aattcagaac attatttgca
      481 caagaaatga ttaaaaataa tattctcatt ccctgcatag cgcaatcatt tgctcactcc
      541 gatacagaat tgcaattgac aagcgatgcc ttgcacaaaa gtttgaaaac ttataagcag
      601 gcaatagaaa atggcattga taattatttg gtaggtccgg caataaaacc tgtttttaga
      661 aaatacaatt agcaaaatga atctatccaa taataatatt atagtcaccg gcagcgaagg
      721 tttgctcggc aaagccatta tagcttgtat cacacaaaat ggcggcaatg ccatctgtat
      781 agatatcgct gacagcagtg cgaatgaaaa ttatttcaaa tgcaatgtta ccgatgaggc
      841 agaagtaaat tctgttttcg acactataat taataaatat gaaaaaattg acggacttgt
      901 aaattgcgca tatccgagaa ccagtgattg gggaaaatat ttcgacaatg aatcgctgga
      961 gagctggaat aaaaatattg aactgcaaac gtcaaatgtt tttctcacaa taaaaaaact
     1021 ggcacctcat ttcgtcaaaa ataaaaatgg cagtgttgtg aacattgctt cgatatatgg
     1081 tgtggtagga aataattttt cactctatga aaatactact atcacttcgc catccgctta
     1141 tactttaata aaaggtggca ttataaattt cacaaagtac cttgcatcct tttacggcaa
     1201 acataatata cgattcaact gtgtttcgcc cggaggaatt tacaataacc aaaacgagca
     1261 gtttgtaaaa aactataatt ccatggttcc attacaaaga atgggcactc ctgcagacat
     1321 tgcgccatcg gtaatttttt tattgagccc tgcaacagca tacatcactg ggcacaattt
     1381 aattgtagat ggcggctgga ctattatata atgccacaat aaaatgaaag aaaataataa
     1441 caaaataaaa gtagtagtta caggtgccga tggttttatt ggcagccacc ttaccgaaca
     1501 tttaatagat tgcggatata atgttacagc catggtgttg tacaattcgt ttggctataa
     1561 tgggtggctc gaaaatttag cacccgaaaa attatcacag ataaaaattg taagtggcga
     1621 tgtgcgcgat taccatagtg tgctgcaagc cacacgaaat gccgatatca tttttcatct
     1681 tgctgcatta attgcaatac catatagcta caccgcaccc gatagttatg ttgatacaaa
     1741 tattaaaggt actcttaata ttgtgcaagc agcgcgcgag cataaaacat cgcgtgtaat
     1801 agtaacttcc acttccgaag tttatggaac agcaaagtac gttcccatag atgaggttca
     1861 cccgttgcaa ggtcagtcac catattctgc atctaaaatt ggtgccgata aaattgccga
     1921 atcattttat cgctcatttc aattgccact tactatagta aggcctttca atacttacgg
     1981 accacgtcaa tcggcacgcg ccattatacc aacaattatt tctcaattac taaataacga
     2041 aacagaaata aaaattggca acatcacccc tacccgcgat tttgtttttg taaaagacac
     2101 tgcaacagga tttgaaaaaa ttgctaacgc gaatgatgcc ataggtgaag aaataaacat
     2161 tgctactcaa caggaaataa gcattggcaa tctggcacaa atgcttatta caaaaataaa
     2221 tcctgaggca aaaataatta ccgatgaaca aagacttcgt cccaacaaca gcgaagtaga
     2281 acgattgctg ggcagcgctg taaaactttt aaaa
//
LOCUS       DAORQA010000283         2314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10430_length_2314_cov_8.644976, whole genome shotgun sequence.
ACCESSION   DAORQA010000283 DAORQA010000000
VERSION     DAORQA010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10430_length_2314_cov_8.644976"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(385..795)
                     /locus_tag="PK736_09095"
     CDS             complement(385..795)
                     /locus_tag="PK736_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010179861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HRC33581.1"
                     /translation="MSEKNNQTFTDTVSKVKEFHAVFGLEFHEQPIASVDEKIIALRH
                     RLMAEENDEYLEAAQQGNLELIADALGDKLYILCGTILAHGLQHKIIDVFNEIHRSNM
                     SKLDDEGNPIYREDGKIMKSNNYFLPDIKAMLGS"
     gene            complement(816..>2083)
                     /locus_tag="PK736_09100"
     CDS             complement(816..>2083)
                     /locus_tag="PK736_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRC33582.1"
                     /translation="ISIENKLPIVYLVDSAGVYLPMQDEIFPDKEHFGRIFRNNAIMS
                     GMGIIQIAAIMGSCVAGGAYLPIMSDEAMIVDKTGSVFLAGSYLVKSAIGEDIDNETL
                     GGATSQCEISGVTDNKFENDEACLNYIRDIFSKLGGFTKAGFNRAPAMKPKTDIKSVY
                     GILPSERDKSYDMHELLQCLIDNGDFQEYKEKYGQSILCGYARVDGWSVGIVANQRKV
                     VKSKKGEMQFGGVIYSDSADKAARFIMNCNQRKIPLVFIQDVTGFMVGSRSEQGGIIK
                     DGAKLVNAMSNSVVPKFTIVVGNSYGAGNYAMCGKAYDPRLMVAWPTARIAVMGGAQA
                     AKVLLQIQVQALKSQGKTELTTDEENEMLAKITEKYNEQLSPYYAASRLWVDNIIDPV
                     ETRTWISMGIEAADHNPITKAYNVGVIQT"
     assembly_gap    2084..2099
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2100..>2314)
                     /locus_tag="PK736_09105"
     CDS             complement(<2100..>2314)
                     /locus_tag="PK736_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRC33583.1"
                     /translation="SYLLDKDTPQIEIGTFAADGMYKEHGGCPCAGVVVVMGYISGRQ
                     AIVVANDATVKAGAWFPMTGKKNLRAQ"
BASE COUNT          669 a          492 c          405 g          732 t
ORIGIN      
        1 gtaattatct atttctacca aagggtaaaa tgttaacgga ataaatttct taatattgtg
       61 ttaagaataa acgtggctca atatgcctac ccatcaacca aaattactgc ataatggttg
      121 taagtgaagt tggggaggca tatagctaga aattaaaatt aaaaaaagaa tcacattgct
      181 gtgatatttt atgagcaaaa aaaagtgaga acaaaatgtg gaacaaaagg gactttgatg
      241 ctgacggttt ttgattgtta ccaaaatgtc ttcacactta gacttttgaa cgaaatatta
      301 aaagttaaat taacgagcat gcttgctggc tttatccttt gtcatattaa tttagagtga
      361 tataaacgga aataaaatct gttgctatga acccaacatt gccttaatat ccggcagaaa
      421 ataattattc gatttcatta tttttccgtc ttcgcgataa atgggatttc cttcgtcatc
      481 gagtttgctc atgttgctac ggtgtatttc gttaaacaca tcaatgattt tatgttgcaa
      541 cccatgtgct aatattgttc cgcaaaggat atacaattta tcgccaagtg catcggctat
      601 taattctaag tttccctgtt gtgcggcttc gaggtattca tcattttctt cggccatgag
      661 gcgatgacgc agcgcaatta ttttttcatc aacacttgca attggctgct catgaaactc
      721 caaaccaaaa acggcatgaa actcttttac tttactaacg gtatctgtaa acgtttgatt
      781 atttttttct gacatgatct ttttacaaat aaattttaag tttgaataac gccaacgtta
      841 tatgcttttg ttattggatt atggtcggca gcttctatgc ccatcgaaat ccaggtgcgg
      901 gtttcaacag ggtcaattat attatcgacc cacaagcgcg atgcagcata gtagggcgat
      961 aattgttcat tatatttctc ggtgattttt gccagcattt cgttttcttc atcagttgtc
     1021 agttcggttt tgccttgcga cttaagtgcc tgcacttgta tttgcaataa tacttttgct
     1081 gcttgtgcac cacccattac tgctatgcgg gctgtgggcc atgccaccat caatcgtggg
     1141 tcgtaagctt tgccacacat tgcataatta ccggcaccgt atgaattgcc aacaacaatt
     1201 gtaaacttgg gcacaacact attactcatt gcatttacaa gttttgcacc gtcttttatt
     1261 atgccgcctt gttcgctgcg cgagccaacc ataaagccgg taacatcttg tataaacact
     1321 aaaggaattt ttcgctgatt gcaattcatg ataaatcgcg cagctttatc ggcactatcc
     1381 gaataaatta caccaccaaa ttgcatttcg ccttttttac tttttactac tttgcgttga
     1441 ttggcaacta taccaacact ccaaccatca acacgtgcat aaccacataa aattgattgg
     1501 ccatattttt ctttatattc ctgaaaatca ccgttatcaa tcagacattg tagcaactca
     1561 tgcatatcgt acgatttatc gcgttcagaa gggagtatac cataaacgct tttaatatcc
     1621 gttttgggtt tcattgcagg tgcgcggtta aatccggctt tagtaaaacc acccaacttc
     1681 gaaaaaatgt cgcgaatgta attgaggcaa gcttcatcgt tttcaaattt attatctgta
     1741 acgcccgata tttcgcattg cgaagtagcg ccaccaagcg tttcattatc tatgtcttca
     1801 cctatggctg attttacaag atagctaccc gcaaggaaaa ccgaccctgt cttatcaaca
     1861 atcatagcct catcgctcat aataggaagg taagcaccac ctgcaacaca gctacccata
     1921 attgcagcta tctgtataat tcccattccc gacatgattg cattgttacg aaaaatgcgg
     1981 ccaaaatgtt ctttgtcggg aaaaatttcg tcctgcatgg gcagataaac accggcacta
     2041 tcaacaagat aaacaatggg caatttattt tctatagaaa tttnnnnnnn nnnnnnnnnc
     2101 tgagcacgaa gatttttttt gcccgtcatg ggaaaccatg caccggcttt cactgtagca
     2161 tcgttagcaa ctacaattgc ctgtcttccg ctaatataac ccatcaccac aacaacgcct
     2221 gcacatgggc atccaccgtg ctccttatac ataccatcgg cagcaaatgt tcctatttca
     2281 atttgaggtg tatccttgtc gagcaggtaa ctta
//
LOCUS       DAORQA010000284         2301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10514_length_2301_cov_5.060107, whole genome shotgun sequence.
ACCESSION   DAORQA010000284 DAORQA010000000
VERSION     DAORQA010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10514_length_2301_cov_5.060107"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1403
                     /locus_tag="PK736_09110"
     CDS             213..1403
                     /locus_tag="PK736_09110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HRC33584.1"
                     /translation="MIKERFIKIKNLLLQENAVAIAFFTITLLATFAQLLKPTHAGEY
                     TRYNNYVIFQQSFFHFIHHQDLYQLYPAEHWDYYKYSPTFALLMAPLALLPTRLGFIL
                     WNMLNAIVLFFSIRALPIAKLNQSKILWFIIPEMVISLQNSQSNALIAGLLIFTFAFL
                     EKGKFAFAALCVALSFYIKIFGILAVILFLFYTKWWQHIFKIVIIFLLLAGIPLLVIN
                     WQQFVFLYKSWLHLLAMDESASIGYSVRGWLFSWIGVDIPNIILVVTGMVLLLFPLVK
                     KSLFSQFQFRLFYLSAIMIWIIIFNHKAESPTFIIAVAGFALFYFNNEKLHINEILFW
                     AVLIFTQLAHSDLFPPTVKEHFMMPYVIKAVPCIAAWAIILFRLMQMPDNKEIGNAMQ
                     VHSK"
     gene            1472..1792
                     /locus_tag="PK736_09115"
     CDS             1472..1792
                     /locus_tag="PK736_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRC33585.1"
                     /translation="MMKRIPAFLKNKYLLSIIVLLVWLTFFDKNNFISQYEYQQELNK
                     LEAEKGYYQSEIENNKRALTILQNDSVALEKYARETFLFKKANEDLFVIVPEHGSETT
                     DYKE"
     gene            1890..>2301
                     /locus_tag="PK736_09120"
     CDS             1890..>2301
                     /locus_tag="PK736_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33586.1"
                     /translation="MKQYLLIIFISSFVYSGSCYGQNLFTPRSYDRSISKQTRDTNGI
                     PGNNYWQNTANYNLNISIIPAAKLLTGKATITYYNNSPDTLKRILIKLFQNLHTPYSA
                     KAHDVDTDYFTGGMEITKCLVGGKNIYINNDSLLQ"
BASE COUNT          715 a          395 c          387 g          804 t
ORIGIN      
        1 aagtaatgcg atggacatta aaaagaattg tctcttatta ataaattaaa aaaatttcgg
       61 ctacaaaagt tttcatcata ctttataaaa attaatggca ttgagaattt ctcaatcaaa
      121 tttttgcgcc atagatttat tatgttgatt tgtgcttata aatattttgg ttcagtttaa
      181 agcataaagt gttttaactg cactttcgta ttatgataaa agaacgcttc attaaaatta
      241 aaaatttgtt gctgcaggaa aatgctgtgg ccatcgcttt ttttaccatc acactgttgg
      301 caacatttgc ccaattacta aaacccactc atgccggtga atatacccgc tataacaatt
      361 atgttatttt tcagcagtcg ttttttcact ttatacatca tcaggatttg tatcaactgt
      421 atcctgccga gcattgggat tattataaat acagccccac gtttgctttg ctgatggcac
      481 cattggcatt attaccaaca aggcttgggt ttattttgtg gaatatgctc aacgccattg
      541 ttttgttttt tagcattcgt gcattaccaa ttgcaaaact aaaccaatcg aaaatattgt
      601 ggttcattat tcccgaaatg gtcattagct tacaaaattc gcaaagcaat gcgcttattg
      661 ccgggctgtt gatttttact tttgcgtttc tcgaaaaagg aaaattcgcc tttgcagcct
      721 tatgtgtggc attaagtttt tacatcaaaa tattcggcat acttgctgta attttgtttt
      781 tattttatac caaatggtgg caacacattt ttaaaatcgt aatcattttt ttgttgctgg
      841 ctggtattcc attgttggta ataaactggc agcagtttgt gtttttgtat aaaagctggt
      901 tgcatttgct tgccatggac gaaagtgcct caatcggata ctcggtgcgc ggttggctat
      961 tttcatggat tggtgtcgat attccaaata taattttggt ggtaaccgga atggtgctcc
     1021 ttttgtttcc tttggtgaaa aaatctttgt tttcacaatt tcaatttcgg ttgttttatt
     1081 tatcggcaat tatgatttgg ataataattt ttaatcacaa agccgagtcg ccaactttta
     1141 ttatagccgt tgcagggttt gctttatttt atttcaacaa cgaaaaattg cacatcaatg
     1201 aaattttgtt ttgggcagtt ttaattttca ctcaacttgc acattccgat ttgtttccgc
     1261 ccacggtgaa agaacatttt atgatgcctt atgttattaa agctgtacca tgtattgctg
     1321 catgggctat tattcttttt cgcctgatgc aaatgcccga taataaagag attggcaatg
     1381 ccatgcaagt acacagtaag taacatgatt gtgttgaaaa caacatatga taattttagg
     1441 atagtatcac ataaaagatt ttatttgtaa catgatgaaa cgtattccgg catttttaaa
     1501 aaacaaatac ctgctatcga taattgtgtt attggtatgg ctcacttttt tcgacaagaa
     1561 taattttatt tctcaatacg aataccagca agaattaaat aaactcgaag ccgagaaagg
     1621 atattaccaa agtgaaattg aaaacaataa gcgtgcactt accattttgc aaaatgattc
     1681 ggtagcattg gaaaaatatg cccgtgaaac ttttctgttt aaaaaagcca acgaagattt
     1741 gtttgttatt gttcccgagc atggtagcga aaccaccgac tataaagagt aatcttcgcc
     1801 aactttagaa taggttttga gcctttgttt ttttataaat tagcaacatg accatttata
     1861 aaaacctgtt tccggaaatt aaaaatgctt tgaaacagta tcttctaatt attttcatct
     1921 catccttcgt atacagtggt agttgctatg gtcaaaattt atttactcca cgctcttacg
     1981 accgaagcat atcaaaacaa acacgcgata ctaacggcat acccggaaat aattactggc
     2041 aaaatactgc aaattacaat ttgaatattt cgattatacc tgccgcgaaa ttgcttacag
     2101 gcaaagcaac tattacatat tacaacaata gtcccgatac attgaaaaga attttgataa
     2161 aactgtttca aaatttacac acgccatatt cggctaaagc acatgatgta gataccgatt
     2221 attttaccgg tggcatggaa attactaaat gccttgttgg cggaaaaaat atttacatca
     2281 ataacgattc gcttttacaa t
//
LOCUS       DAORQA010000285         2291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10562_length_2291_cov_13.753131, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000285 DAORQA010000000
VERSION     DAORQA010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10562_length_2291_cov_13.753131"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..675
                     /locus_tag="PK736_09125"
     CDS             286..675
                     /locus_tag="PK736_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33587.1"
                     /translation="MKGICTKMKTTKLFSVIVFHLIILSFSVRAQTGMDCDSFCVESI
                     IIDTNVTQGFIVAIKNNNTEYVNYPTIDVIDKTTNTVIASGMFIYFANIQGQSLAYQV
                     FSNTSIIPDSAIASITDNIWNITYQLS"
     gene            1281..>2291
                     /locus_tag="PK736_09130"
     CDS             1281..>2291
                     /locus_tag="PK736_09130"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC33588.1"
                     /translation="MITRNIYSTVAKALKRFPAVAILGPRQVGKTTLVKQVSKSLKGE
                     VLFLDLEKDSDYNKLNRDAEDFLSQYVKSTVVLDEIQRMPSLFVLLRPLIDENRKPAR
                     YLITGSASPELLKGATESLAGRISYLHLYPINVMELPEKISIHQHWLRGGFPVALTAK
                     SNDFAFDWLGNFITNYIERDLPNLFDVQFSPILMKKMWQMMAHWQSQIINLEDISRSL
                     SVGATTVKRYFDFLEGAFIIHRLPPFYVNINKRLVKSPKLYILDSGMLHRLLHITSNK
                     ELAGHPFSGASWEAYVVSQLIYNMPGHLTAYFYRTHTGAECDVVLAQGHIIKYCIEIK
                     LGK"
BASE COUNT          805 a          388 c          423 g          675 t
ORIGIN      
        1 acaattattt gtgaggtatg cgatatatca gggcgaatta tttttcacaa aattgttgtg
       61 gcaaaaaaag ggtttaacga gtttgaaatt gatttaaccg aaaacacaaa aggaatgtac
      121 attctacatt tgcatgcaga agaagaatcg agagctataa agttagtttt ggaataacat
      181 gcaatggtat aaagaagtta acatatataa ctaactgtta agtcaacaaa catttttttg
      241 tacagggtaa tgaagatggt aaaacaatta aaaaaagaaa tataaatgaa aggaatatgt
      301 acaaagatga aaacaacaaa attattttcc gtcatcgtct tccatcttat aatacttagc
      361 ttttcggttc gagcacaaac aggaatggat tgcgattcgt tttgtgtaga atcgataata
      421 atagatacca atgtaacaca gggatttatt gtcgccataa aaaataacaa tacagaatat
      481 gtaaactatc caacaattga tgtgatagat aaaaccacaa atacggttat agccagcggc
      541 atgttcattt actttgcaaa tattcagggt caaagtttag catatcaggt tttttcaaac
      601 acttcaataa ttcccgattc tgccattgca tctataaccg acaatatttg gaatataact
      661 tatcaactgt cttagccttg cacagttaca aatgcagttc aagaaataaa tgcaaacaaa
      721 ttgcaatgtt agtgaagcgg aatttttttg atattgagcc aacaatcgaa aaatgaaatt
      781 aaggaaataa aattatttga tctcaatggc aatgtaattt ccacagacat gaaagtgata
      841 aataactata ctcaaatagg cattagagaa aataccgcat aaggaatatt tattgccgga
      901 agacttgtgc aagaattagc gttttatatt gaaaagaatg gaaacttcat tcttgaagat
      961 tcaaattgta aaaaagcaga atctaattat cagcaaaact aaaaaacaga tacatgaacc
     1021 aatagatatg acagcgcaac aacaaaggct atactttata aaattgcaaa caactcaagg
     1081 aaattgggta aagaaaatta cggtaataaa ataagagaat aatttatttt ccaaaaaaag
     1141 ccatcttaaa ggatggctct ttttgtttca atatgcaggt tggttcaaaa cactgcgtca
     1201 ataatttaaa atcacaacct tgaaaatcca ctttcaaata gtggattttc aatgttggca
     1261 aaataatata tatttgcgca atgattacta gaaatatata ttcgacagta gccaaagcgc
     1321 tcaaacgttt ccctgctgtg gctatattag gcccacggca ggttggtaaa accaccttgg
     1381 taaaacaggt ttctaaatcg ctgaagggcg aagtgctttt cctcgacctt gaaaaagatt
     1441 ccgattataa taaacttaac cgcgatgccg aagatttctt atcgcaatat gtaaaaagca
     1501 ctgtagtgct tgacgagatt caacgcatgc catcgctatt tgttttgttg cggccattga
     1561 tagatgaaaa ccgcaagcct gctcgttatc ttatcactgg ttcggcatcg cccgagttgc
     1621 ttaaaggtgc gaccgaaagt ttagcaggtc gtatttctta tctccactta tatcccataa
     1681 acgttatgga attgccggaa aaaatcagca tccatcagca ttggttgcgt gggggatttc
     1741 cggtagccct tacagcaaag tcaaatgatt tcgccttcga ttggcttggc aactttatta
     1801 ccaattacat agagcgcgac ttgccaaatt tatttgatgt gcaattttca ccgatactga
     1861 tgaaaaaaat gtggcaaatg atggcgcatt ggcaaagtca aattattaat ttggaggata
     1921 tttcgcgttc tctttcggta ggtgctacta ctgtaaagcg ctattttgat tttcttgaag
     1981 gtgcatttat cattcacagg ctgccaccat tttatgtaaa tattaataaa cgcttagtaa
     2041 agtcacctaa attgtatatc ctcgattcgg gtatgctgca ccgccttttg cacattacaa
     2101 gcaataaaga attggccggc catccctttt cgggggcatc gtgggaggct tatgtagtaa
     2161 gccaattgat atacaatatg ccgggtcatc ttacagcata tttctatcgc actcacacag
     2221 gtgccgagtg tgatgtggtg ctggcgcaag gccacataat aaaatattgt atagaaatta
     2281 aattaggaaa a
//
LOCUS       DAORQA010000286         2288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10585_length_2288_cov_6.371697, whole genome shotgun sequence.
ACCESSION   DAORQA010000286 DAORQA010000000
VERSION     DAORQA010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10585_length_2288_cov_6.371697"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="PK736_09135"
     CDS             complement(<1..1261)
                     /locus_tag="PK736_09135"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRC33589.1"
                     /translation="MVMQHINLQNIKYVYMLGIGGIGMSALARYFHAMGMQVAGYDKT
                     ETTLTQELISENIAVQYDDDVKELPAFLSNDNHESLLVIYTPAMPSDLKLLNYLKLQQ
                     TLYKRSQVLGMIAANTSTVAVAGTHGKTTTSCMVTHLLKTKGIQCSAFLGGISKNYNT
                     NFIAGSFNQTPHTVVVEADEFDRSFLTLFPKYAIITSTDADHLDIYNTADELLKSYNQ
                     FASQVAPDGKLVCRLGLKVNVPNMITYAINEPADARGYNIRIENHEYVFDYSYNGNNI
                     NNIRSGMPGRHNVENVVAAITIALLHGAGVDEIKQGVSSFTGVKRRFDYQIKSKDVVY
                     IDDYAHHPSEIEAAIKSSKELYPGMHTTVIFQPHLYSRTRDFADGFASSLSLANKTIL
                     LPIYPARELPIANVNSEMIKNKMTGECE"
     assembly_gap    1391..1407
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1408..2235)
                     /locus_tag="PK736_09140"
     CDS             complement(<1408..2235)
                     /locus_tag="PK736_09140"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRC33590.1"
                     /translation="MEKVPAAGFKIIGLNIAGLQRSFSLKNFLFPFKLISSLLQAKKI
                     IKDFKPDAAVGVGGYASGPLLYMAASANVPCIIQEQNSYPGITNKMLAKKASRICVAY
                     QGMEKYFPADKLMLSGNPVREDLLNITNKSAEAFEYFGLDKNIKTILIVGGSLGARTI
                     NQSIQQSLDKLFEAGVQVIWQTGKAFYDSAVESARKYESKIKVHAFISKMDYAFSVAH
                     VIVSRAGASTISELCIVGKPCILIPSPNVAEDHQTKNAMALVNTNAALMVTDAACQND
                     "
BASE COUNT          645 a          459 c          432 g          735 t
ORIGIN      
        1 gctcacattc gcctgtcatt ttatttttta tcatttccga atttacattt gctattggca
       61 attcgcgtgc aggatatatt ggcaataaaa tagttttgtt ggcaagcgat aaacttgagg
      121 caaacccatc ggcaaaatcg cgtgtgcgtg aatacaaatg cggttgaaaa attacggtag
      181 tatgcatacc gggataaagt tcctttgaac ttttaatagc ggcttctatt tcggaaggat
      241 ggtgtgcata gtcatcaatg taaactacat cttttgattt tatctgataa tcaaatcgtc
      301 ttttaacacc tgtaaacgaa ctcacaccct gctttatttc atcgacaccg gcaccatgca
      361 gcagcgcaat agtgattgca gccaccacat tttccacatt atgacggccg ggcattccac
      421 tacgtatatt gtttatgttg ttgccattat agctatagtc aaaaacatac tcatggtttt
      481 caattctaat attatatcca cgggcatcgg caggttcgtt tatagcatag gttatcatgt
      541 taggaacatt aaccttcaaa cccaaacggc atacaagttt accatcgggg gctacctgtg
      601 aagcaaattg gttgtagctt ttcaataact catctgcagt gttgtaaatg tcaagatgat
      661 cggcatcggt cgaagtaatg attgcgtatt ttggaaacaa tgtcagaaac gaacgatcaa
      721 attcatcggc ttctactact acagtatgtg gagtttgatt aaaggaaccg gcaataaaat
      781 tggtattata atttttcgaa atgcctccca ggaaagcaga acattgtatg cctttggttt
      841 taagcaaatg tgttaccatg cacgaagtag ttgttttgcc atgtgttccg gcaacagcta
      901 cagtacttgt gtttgccgct atcattccca atacctgcga gcgcttatat aatgtttgtt
      961 gtaacttaag atagttcagc aattttaaat cggacggcat ggccggagta taaatcacca
     1021 ataaagattc atgattatca tttgataaaa atgccggtaa ttcttttaca tcatcgtcat
     1081 attgcactgc aatgttttcg gatatgagtt cctgagtaag tgtagtttcg gttttatcgt
     1141 aacctgccac ttgcataccc atagcatgaa agtaacgggc aagcgcactc atccctatac
     1201 cgccaatacc aagcatgtac acatatttta tattttgcag attgatatgt tgcatcacca
     1261 tttttgtatg atagaaatta cttcgtttgc aatagcgttt gcacttccgg gcaaagcaag
     1321 ttttttaata tttgttgcca gttgagtttg cagattgata tcgttcaaga gacttttcaa
     1381 ttccgctatt nnnnnnnnnn nnnnnnnatc attttggcaa gcagcatccg ttaccatcaa
     1441 tgctgcattt gtatttacaa gcgccatggc attttttgtt tgatgatctt cggccacatt
     1501 gggcgatggt ataagtatac aaggcttgcc aacaatgcac agttccgaaa ttgtacttgc
     1561 tccagcacgc gatacaatta catgggcaac actaaatgca taatccattt tacttataaa
     1621 tgcatgcact ttaattttgc tttcatattt cctggcactc tcaactgccg aatcataaaa
     1681 agctttaccg gtttgccata ttacctgcac tccggcttcg aataatttat ctaacgattg
     1741 ctgtatactt tggttgatgg ttcgcgcgcc aaggctgccg ccaacaataa gaatggtttt
     1801 aatattttta tcaaggccga aatattcaaa tgcttcagca cttttattag taatgtttaa
     1861 taaatcttcg cgaacggggt ttcccgaaag cattaactta tcggcaggaa aatatttttc
     1921 cattccctga tatgcgacac aaattcttga tgctttcttt gcgagcattt tatttgtaat
     1981 gcctgggtag gagttttgct cctgaataat gcacggcaca ttagctgaag cagccatgta
     2041 taataacggt ccgcttgcgt aaccgccaac accaacagcg gcatcaggtt taaaatcttt
     2101 tattattttt tttgcctgta ataaactgct gattaatttg aaagggaaaa gaaaattttt
     2161 taacgaaaac gaacgttgca aaccggcaat gttgagtcca ataattttga aacctgcggc
     2221 cggcactttc tccatttcct gtgaccggac aacctttgcc acttgtaagc atgggaggaa
     2281 cttctatg
//
LOCUS       DAORQA010000287         2276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10651_length_2276_cov_4.820351, whole genome shotgun sequence.
ACCESSION   DAORQA010000287 DAORQA010000000
VERSION     DAORQA010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10651_length_2276_cov_4.820351"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>665
                     /locus_tag="PK736_09145"
     CDS             <1..>665
                     /locus_tag="PK736_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="G-D-S-L family lipolytic protein"
                     /protein_id="HRC33591.1"
                     /translation="AYSAGTANFTTYVAVGNSLTAGYQDNALSLAGQQNAYPAILSSV
                     FSKVGGATEFNTPYLTGVDASNGVNPAPSGPNFFRTLSKLILKKDVMDCLGNKSLAPS
                     LFAAPIDNNQAFYFTRVDSTGKKHFHNMGVPGAKVRDVINTLYGNALLGGNPFYGRFA
                     TNPGTSSMLSDAASANPTFFTLWIGNNDVLGYATAGGTGAIGGLLPSDITPVDSFESA
                     YAN"
     assembly_gap    666..675
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <676..1351
                     /locus_tag="PK736_09150"
     CDS             <676..1351
                     /locus_tag="PK736_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33592.1"
                     /translation="VLTSNGAKGACANIPDVASVPFFTTVPYNAITLTSQAQVDGLTA
                     AWAGYNQAATGAGLPLITWKLGPNPMVIKDASFPISGIRQIKPTELVCLTIPQDSLKC
                     ANWGTAVPIESFYVLTDAEIANINTYTNQYNTIIKKYADMKGLAYVDMRNYFKTFVDG
                     NIIFNGVTYTTEFVKGGVFSLDGVHPNQRGYSLIANEWIRNINSKFGSNLPGVDVNNY
                     AGVLFP"
     gene            1408..>2276
                     /locus_tag="PK736_09155"
     CDS             1408..>2276
                     /locus_tag="PK736_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HRC33593.1"
                     /translation="MNIDINNNTVLVAPLNWGLGHAARCVPVINKLLANNCTVIIASD
                     GNALQFLKTAFPLLQYYELPGYNIQYPANKKMTTSLLWQFLFVPNTIKKEHNAITKIV
                     AIEKPQYIISDNRYGVFHTDCTNIIITHQLSFKFEGIEKTFETLANSTVQHYVNKFNE
                     CWIPDFENEKLNFTGFLSSPKLKITKKYIGVLSSLYDASFDENTIPLYDFCIIITGPE
                     KQRTLFENMILEKLISTTYTAIVVRSSDLPLLPKYKTKKIQYINFANSNEVAQCMQQS
                     QFVVSRAGYTTIM"
BASE COUNT          707 a          426 c          427 g          706 t
ORIGIN      
        1 gcctatagtg cgggcacggc aaattttaca acctatgttg cagtaggaaa ttcgcttaca
       61 gccggctatc aggataatgc attatcattg gcagggcaac aaaatgctta tcctgctatt
      121 ctcagttcgg tgtttagtaa agtgggaggt gcaacagaat ttaatacacc ctatcttaca
      181 ggtgttgatg caagcaatgg agtaaatcct gcaccttccg gtccaaattt tttccgcact
      241 ttatcgaaac ttattttgaa aaaagatgtg atggattgtc ttggaaacaa atctttagcc
      301 ccttcattat ttgctgcccc aattgacaat aatcaggcat tttattttac acgtgttgat
      361 tcaacaggca aaaaacattt tcacaatatg ggtgtacccg gtgcaaaggt tcgcgatgta
      421 attaatacat tatatggcaa tgcattgtta ggtggtaatc ctttctatgg aaggtttgca
      481 accaatcccg gcacttcatc tatgttatcg gatgcagcat ctgccaaccc cacatttttt
      541 actttatgga ttggcaataa cgatgtgctt ggttatgcca ctgcaggtgg tacaggtgcc
      601 attggtggac ttttaccaag cgatataaca cctgtagatt ccttcgaaag tgcttatgct
      661 aatatnnnnn nnnnntgtat taacatctaa tggtgctaaa ggtgcatgtg caaatattcc
      721 cgatgtagcc tcggtgccat tttttacaac tgtaccttac aatgcaatta cgcttacaag
      781 tcaggcacag gtggatggtc ttactgctgc atgggcaggt tataatcagg ctgcgactgg
      841 tgctggtctt cctttaatta catggaagtt aggaccaaac ccaatggtga taaaagatgc
      901 cagtttccct atatcgggaa tacgccaaat aaaaccaaca gaattggttt gcttaacaat
      961 acctcaagac tcgcttaaat gcgccaattg gggaactgca gtacccatag aaagttttta
     1021 tgttttaaca gatgccgaaa ttgcaaacat caatacttac acaaatcaat acaacaccat
     1081 cataaaaaaa tatgctgata tgaaaggatt ggcttatgtt gatatgcgaa attatttcaa
     1141 aacttttgtg gatggtaata ttattttcaa tggcgtaact tatactacag agtttgtaaa
     1201 aggtggtgtt ttttcgctcg atggtgttca tccaaatcaa cgcggttatt cgcttattgc
     1261 caatgaatgg atacgtaata taaattccaa atttggttct aacttaccgg gtgtagatgt
     1321 aaataactat gcaggagttt tgtttccgta atatcagtta aatataattt atagccatgc
     1381 cggttttcgg tgtggctttt tttttgaatg aatattgaca taaataacaa caccgtatta
     1441 gttgctccac tcaactgggg tcttggtcat gctgcgcggt gtgtgcccgt tataaataaa
     1501 ttgctggcaa ataattgcac cgtcattatt gcttcggacg gtaatgcatt acaatttctg
     1561 aaaactgcat tcccgctttt acagtattac gaattgccgg gttacaacat acagtatcct
     1621 gcaaataaaa aaatgacgac ctccctttta tggcaatttt tgtttgtgcc aaatacaatt
     1681 aaaaaagagc acaatgccat tacaaaaatt gttgcaattg aaaaaccaca atacattatt
     1741 tcggataacc gatatggagt gtttcataca gattgtacca atatcattat cacacatcaa
     1801 ttaagtttca agtttgaagg tatcgaaaaa actttcgaaa cacttgccaa tagtacagtg
     1861 cagcattatg taaataaatt caacgagtgt tggattccgg attttgagaa cgaaaaacta
     1921 aattttacag gatttctttc gtcaccaaaa ttaaaaatca ccaaaaaata tattggcgtg
     1981 ctttcatcgt tatacgatgc aagttttgat gaaaatacaa ttccgcttta cgatttctgc
     2041 atcataatta caggccccga aaagcaacga acattgttcg aaaatatgat tctcgaaaaa
     2101 ttgatttcta ctacgtatac agctattgtt gttcgaagca gcgatttgcc attgttgcca
     2161 aaatataaaa ccaaaaaaat tcaatacata aactttgcaa actcgaatga ggttgcccaa
     2221 tgtatgcagc agtcacaatt tgtggttagc cgtgcgggct ataccactat tatgga
//
LOCUS       DAORQA010000288         2266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10696_length_2266_cov_8.936680, whole genome shotgun sequence.
ACCESSION   DAORQA010000288 DAORQA010000000
VERSION     DAORQA010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10696_length_2266_cov_8.936680"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="PK736_09160"
     CDS             <1..1094
                     /locus_tag="PK736_09160"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRC33594.1"
                     /translation="SFIGVILPILLAIAFPAKYPHWVSQYKILGYLSSIGFSLYPLTA
                     AYVIGKRSGINQKQLLAHVNQIEKLSQENLQKEQEKKKILEEQNSQLELKVKERTGEL
                     AAKNDLITLKNKEITDSLIYAKRIQAAILPDKKMMLNSFDDAFILYLPKDIVSGDFYS
                     FMRKENKIIIAAADCTGHGVAGAFMSMIGTSLLNQIINEKNITAPASILEHLNDGIIT
                     ALKQKESDTNDGMDISMCTFDLDAMTGEFAGANRPLLLLRKGELTTYKPNKLPIGGLQ
                     IVADKKYTTTQIDLVKDDHIYFYTDGYADQFGGTMGKKLMIKKFKESLLAIENKTMSE
                     QKDHLRRFFYEWKGHHDQVDDVLVIGIKI"
     gene            1175..>2266
                     /locus_tag="PK736_09165"
     CDS             1175..>2266
                     /locus_tag="PK736_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33595.1"
                     /translation="MRKQIFYTFFFIVFFVLKSMGQNEDEINDRSSVPVNINLGDDDD
                     DTTDTDKPAVLLLDSLWKTKKGDDMLWASPTFDDSSWKKKDQDSILRIDNMNAITWFR
                     IPFTADSVACSEPLALSMTHYGTAAEIYLDGKLIANYGKVGNSKNSEEAVLTVESKPV
                     PFLIQQPGRHVFAIRFSNFHASEAKIVGITMGKKFTFSVDKLSNDYDDITDPSRYSAF
                     VFYASIFITLAVVHFLFFIFYRQNIINLYYSLYCLGIFVLIYFIFVVLTSSDFQTIMN
                     FSKPFRFIMGALFIPLVAMLHKIFYGYFRRIFWVIVIIYAAALIAAIFGFYSLAKGLM
                     IATLFTTFVEIIRVIFVSVRKRKDGAWIFA"
BASE COUNT          730 a          378 c          434 g          724 t
ORIGIN      
        1 gttcctttat tggagttata ttgcccattc tacttgctat tgcttttcca gcaaaatatc
       61 ctcattgggt ttcgcaatat aaaattctgg gttatcttag ttcaatcggg ttttcacttt
      121 atcccttaac ggcagcatat gtaataggta aaagaagcgg catcaatcaa aaacaattgc
      181 tggcacatgt aaatcaaatt gaaaaattat cgcaagagaa tttacaaaaa gaacaagaga
      241 agaaaaaaat actcgaagag caaaactccc aattagaatt gaaagtgaaa gaacgcacag
      301 gtgagttggc agcaaaaaac gatttgataa ctttgaagaa taaagaaatt actgatagcc
      361 ttatttatgc caagcgtata caagctgcca tactccccga caaaaaaatg atgctgaata
      421 gctttgatga tgcattcatt ttgtacttgc ctaaggatat agtgagtggc gatttttaca
      481 gctttatgcg gaaagaaaat aaaataataa ttgccgctgc cgattgcaca gggcatggtg
      541 tggccggtgc ttttatgagt atgataggta catcgttgct caatcaaatt attaacgaaa
      601 aaaatattac tgcccctgct tccattctcg aacatcttaa tgacgggatt atcacagccc
      661 tcaaacaaaa agaaagcgac accaatgatg gtatggatat aagcatgtgc actttcgatt
      721 tagatgcaat gacaggagag tttgccgggg ccaacaggcc attattacta ttgcgcaaag
      781 gagaattaac aacttacaaa ccaaacaaac ttcctatagg tggcttgcaa atagtggccg
      841 ataaaaagta caccaccaca caaatagatt tggtaaaaga cgatcacatt tatttttata
      901 cggatggtta tgccgatcag tttggtggta cgatgggcaa aaaattgatg ataaagaaat
      961 ttaaagaatc gttgcttgcc atcgaaaata aaacaatgag tgaacagaag gatcatttga
     1021 gacgtttttt ttatgaatgg aaaggtcatc atgatcaggt tgatgatgta ttggtaatag
     1081 gtataaaaat atgatgtttg cggtttgata gtataccgta ataaatgtaa aataatatgt
     1141 cgacaaatgt aattgtagaa acaaagttat ccttatgaga aaacaaattt tctatacttt
     1201 ctttttcatc gttttttttg tgcttaaatc aatggggcaa aatgaagatg aaataaacga
     1261 tagaagtagc gtgcctgtaa atattaattt gggtgatgat gatgatgata ctactgacac
     1321 agataaaccc gctgttttgc ttttagattc gttatggaaa acaaaaaaag gcgatgatat
     1381 gctgtgggct tcaccaacat ttgatgatag cagctggaaa aaaaaggacc aggattctat
     1441 tttacgtatc gataatatga atgcgattac ctggtttcgt attcctttca ctgccgacag
     1501 cgttgcatgc agcgaaccac tcgcattgtc aatgacacat tatggtacgg cagcagaaat
     1561 ttatctagac ggaaaactta ttgcaaacta tggcaaagta ggcaacagca aaaatagcga
     1621 ggaagcggtg cttactgtag aaagtaaacc tgttcctttt ttaatacaac aaccgggtcg
     1681 ccatgttttt gctattcggt tttctaattt tcatgcgagc gaggccaaaa ttgttggcat
     1741 caccatgggc aaaaaattta cttttagtgt agataaattg agcaacgact acgatgacat
     1801 taccgaccct tcgcgttatt cagctttcgt tttttatgcc tccattttta ttacgttggc
     1861 agtagtgcat tttctttttt ttatttttta ccgccaaaat attattaacc tctattatag
     1921 tttgtattgt ttgggaatat ttgttttaat ctatttcata tttgtggtgc tcacctcatc
     1981 cgattttcaa accataatga atttctctaa accatttcga tttattatgg gtgcattatt
     2041 tattcctctt gttgccatgt tgcacaaaat attctatggc tatttcagac gtatattctg
     2101 ggttattgtt attatttatg ctgccgctct tattgccgct atattcggtt tttattctct
     2161 tgccaaagga ttaatgatag ccacactttt tacaacattt gtagaaataa ttcgtgtcat
     2221 atttgtttcg gtgcgtaaac gcaaagatgg tgcatggata tttgct
//
LOCUS       DAORQA010000289         2256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10755_length_2256_cov_4.622444, whole genome shotgun sequence.
ACCESSION   DAORQA010000289 DAORQA010000000
VERSION     DAORQA010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10755_length_2256_cov_4.622444"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1180)
                     /locus_tag="PK736_09170"
     CDS             complement(188..1180)
                     /locus_tag="PK736_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HRC33596.1"
                     /translation="MYVNRNIPFEIIWRFGWLNIVLFILYSFVVFSMHNFLLINYQFQ
                     MHIPFALIGPVGVAVAIYTSFKNGQSYDRTWEARKNWGALAGLSKIFANQVMVYVNQK
                     DDSAAKILLHRQLAILNAHKLQLRVKSLHSKKYAPWLHKYYFGTATETDWKNEVASFL
                     EDKEYANIMSKTSKAYHILLMQNQHLAAMRNNNVINEFQHMEMIKVLDGCIENMGKNE
                     RIKTTPFPRQYAFFSKSFIVIFILMLPFGFLDLYNNHYSLASYITMGFIFTIVAWLYI
                     TMEIVGSYSEDPFEHFATDIPMNVICRNVQIDYAEILDEKIIPEKVVPVDGILL"
     assembly_gap    1435..1444
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1445..>2256)
                     /locus_tag="PK736_09175"
     CDS             complement(<1445..>2256)
                     /locus_tag="PK736_09175"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC33597.1"
                     /translation="LLKGNNILNQSAVRGTKGVPNINNRPEGRSGSVAFLDTLQRFYI
                     FGGAVTGNIQGINDVWRLELDPGCVIPTCSSALPVAAFSSSDTLLCEKGSIDFFDNSA
                     GNPTAWNWTFTGGAPSTSTLQNPTGIFFANYGTFNVSLTVTNGAGSNTTSFTVTVVAN
                     PAQPVVTFNGSTICSSPAVSYQWYFNNAPISNATSQCYTPTLLGNYYVIISDSNGCNS
                     ASNIFVANAINNTPAKESGFTLIPNPASDAVQLSFTHAYMGTVNIELYDEKG"
BASE COUNT          701 a          447 c          421 g          677 t
ORIGIN      
        1 tcccttggct gctgataaca ttcgcgcttg gcgcaaaccc caacaccaat ataatttcca
       61 taacctccat ccgctcacct cacatcaaaa tgttatttgc atttgcaaag caaatcaggt
      121 tttattagag taaatgacac aacgtatatt gttacagtta taaatttccg cttttgataa
      181 taggtaatta cagcaatatc ccatcaacag gcacaacttt ttcagggata attttctcgt
      241 ccaatatttc ggcataatca atttgaacat ttcggcaaat tacattcatg ggtatatcgg
      301 tggcaaaatg ttcaaatggg tcttcgctgt aagagcctac aatttccatt gttatataaa
      361 gccaggcaac aattgtaaaa ataaaaccca tagtaatgta acttgccaat gagtaatgat
      421 tgttatacag atcgagaaag ccaaatggca gcatcaaaat aaaaatcaca ataaacgatt
      481 tactgaagaa tgcgtattgg cgtggaaacg gagttgtctt aatgcgttca tttttgccca
      541 tattttcaat acagccatca agcactttta tcatttccat atgctgaaat tcgtttatca
      601 cattattatt tcgcatggca gccaaatgtt ggttttgcat caataagata tgatatgcct
      661 tgcttgtttt gctcataata tttgcatact ccttatcctc aaggaaagag gcaacctcat
      721 ttttccaatc ggtttcagta gccgttccaa aataatattt atgcaaccat ggagcgtact
      781 ttttgctgtg caggcttttt acacgcagtt gcaatttatg cgcattaaga atggcaagtt
      841 gcctgtggag taaaattttg gcagcactat catccttttg atttacataa accataacct
      901 gattggcaaa tatttttgac agccctgcca gtgcacccca attcttacgt gcctcccatg
      961 tacgatcata cgactgccca tttttgaaac tggtataaat ggcaacagct acacccacag
     1021 ggccaatcaa tgcaaagggt atatgcattt ggaattgata gtttataagc aagaaattgt
     1081 gcatggaaaa aacaacaaaa gagtacaaaa taaacagcac aatatttagc cagccaaacc
     1141 gccagattat ttcgaagggt atattccggt taacatacat cgctttgtgg tttttattat
     1201 ttcactacac gagataaatt attagttggt ttttgcgttt tttagaattt atttttcaac
     1261 atgtagtata ccgtaatagt tgaacataaa atactacttt accgagagtt ttttatccat
     1321 taccactttg ccaaacttta tcattacaat gtaatttcct tttggtaaat gtttcaattc
     1381 caattcgtgg ttgaacgaaa aagaatcctt ttgaattttc ttgcttaatg cgatnnnnnn
     1441 nnnncacctt tttcatcgta gagttcaata ttaactgtac ccatatacgc atgagtgaat
     1501 gaaagttgca cagcatcgct ggcgggatta ggtatcaaag taaatcccga ttctttagcc
     1561 ggagtattat ttatggcatt ggctacaaaa atattactgg cactgttgca tccattggaa
     1621 tcggatatga taacatagta attgccaagc agagtagggg tatagcattg cgaagtggca
     1681 ttgcttatag gtgcattatt aaaataccac tgatacgaaa cggcaggtga actgcaaatg
     1741 gtactgccat tgaaagttac aacaggttgg gcggggttgg ctacaacggt aactgtaaac
     1801 gatgtagtat tgctgcccgc accattggtt acagtgagcg aaacattgaa tgtaccatag
     1861 ttggcaaaaa atattcctgt aggattttgc aaagtagaag tggaaggagc gcctcccgta
     1921 aaagtccagt tccatgcagt aggattgccg gcagaattat caaaaaaatc tatcgaacct
     1981 ttctcgcaaa gcaaagtatc gctgctcgaa aatgccgcca caggcaatgc gctgctacaa
     2041 gttggtataa cgcaaccggg gtcaagttcc aatcgccaga catcatttat gccttgtatg
     2101 ttgccggtaa cagcaccacc aaaaatgtaa aaacgctgca aggtatcgag gaatgcaacc
     2161 gaaccagacc ggccttcggg acgattatta atattgggta cgccctttgt tccgcgcaca
     2221 gccgattgat tgaggatatt atttcctttc aataaa
//
LOCUS       DAORQA010000290         2251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10782_length_2251_cov_4.735428, whole genome shotgun sequence.
ACCESSION   DAORQA010000290 DAORQA010000000
VERSION     DAORQA010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10782_length_2251_cov_4.735428"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1138)
                     /locus_tag="PK736_09180"
     CDS             complement(17..1138)
                     /locus_tag="PK736_09180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC33598.1"
                     /translation="MKVVLLGSAHPLRGGLASYNERLATEFQAMGHEVIIYTFSLQYP
                     SFLFPGTSQISDSPAPQHLNIKIKVNSINPFNWIMVGNEIKNLRPDLLIVKFWLPFMG
                     PCFGTICRIVKWNKKTKIVSVLDNVIPHEKRFGDTLFTKYFLKPVDAFVAMSKSVKDD
                     LFTFVTTKKCALNPHPLFDNFGNPVSKNDALDFLHLEKNVPYILFFGFIRDYKGLDWL
                     LQACGSNTLRNANFKLIVAGEFYSDSEKYLQLIKDLNLESKVVLKTDFIKDEEVKYYF
                     CAADMVVQPYKHATQSGVTQICYHFDKPMLVTDVGGLAEIVPHMQAGYVVQPNVEDIA
                     LAIKDFYDNNRQPSFEAFVKNEKQKYAWSKMVKAIESVM"
     gene            complement(1148..2098)
                     /locus_tag="PK736_09185"
     CDS             complement(1148..2098)
                     /locus_tag="PK736_09185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC33599.1"
                     /translation="MDISVVIPLFNEDESLPELFPWIAKVMQANKYSYEVIFIDDGSK
                     DKSWQVIENLAQQNAEVRAIKFRRNYGKSAALQKGFEIAQGDVVITMDADLQDSPDEI
                     PGLYKMIMNDGYDLVSGWKRKRYDPIMKTIPSKLFNKATKIMSGIHNLHDFNCGLKAY
                     RKEVVKNIEVYGEMHRYIPVIAKWQGFAKIGEKEVQHQARKYGVTKFGLDRFINGFLD
                     LLSIFFIGKFGKRPMHFFGTLGTLMFFIGFVFIVLLGVGKMWAVYNLQDARLLTQRPS
                     FYISLTCMVMGTLLFLAGFLGELISRMGVHRNQYQIEKTL"
BASE COUNT          671 a          459 c          400 g          721 t
ORIGIN      
        1 aaaaaacttc aactaattac atcacacttt ctatagcctt caccatttta ctccaggcgt
       61 atttttgttt ttcgtttttt acaaaggctt cgaaacttgg ttgcctgttg ttgtcgtaaa
      121 aatctttaat tgcaagtgca atgtcttcta cgttgggttg caccacgtag cctgcctgca
      181 tgtgaggcac tatttcggca aggccgccta catcggtaac tagcataggt ttgtcgaagt
      241 gataacaaat ttgggttaca ccactttgtg ttgcatgttt gtatggttgc accaccatat
      301 cggcagcgca gaaataatat ttcacttcct catctttaat aaaatcagtt ttaagaacca
      361 ctttgctttc gaggtttaaa tcttttatca gttgcaaata tttttcggag tcggaataaa
      421 attctccggc cactatcagt ttgaaatttg cattgcgcaa tgtattgctg ccacatgctt
      481 gcaacaacca atcgaggcct ttgtaatcgc gtataaagcc aaagaataaa atgtatggaa
      541 catttttttc aagatgtaaa aaatctaaag catcgttttt cgaaaccggg ttaccaaaat
      601 tatcgaacaa aggatgagga ttgagtgcgc acttttttgt tgttacaaat gtgaacaaat
      661 catcttttac cgatttactc atggcaacaa atgcatcaac aggcttgagg aagtattttg
      721 taaaaagggt atcgccaaat cttttttcgt gaggaataac attatcgagc acgcttacga
      781 tttttgtttt tttattccac ttcactattc ggcaaatagt accaaaacaa ggacccataa
      841 acggcagcca aaattttact atcaataaat cgggtctgag attttttatt tcgttaccca
      901 ccattatcca gttgaatggg tttatggagt taacttttat tttaatattc aaatgttgcg
      961 gtgccggtga atcggaaatt tgcgatgtgc ccggaaataa aaatgatgga tactgcaaac
     1021 taaaagtgta gataataacc tcgtgcccca tagcctgaaa ctcggtagca aggcgttcgt
     1081 tgtacgatgc caatcctccg cgtaagggat gtgccgaacc cagcaatact actttcatcg
     1141 ggcagaatta caaagttttt tcaatttgat actggttgcg atgtacgccc atacgcgaaa
     1201 tcagttcgcc aagaaaacct gctaaaaaaa gcagtgtgcc cattaccata caagtaagcg
     1261 aaatataaaa tgagggacgc tgagtgagta ggcgcgcatc ttgcagattg tatactgccc
     1321 acatttttcc tactccaagc agcacaataa aaacaaagcc tataaaaaac ataagtgtac
     1381 caagcgtacc gaaaaagtgc atgggtcgtt ttccaaattt gccaataaaa aatatagaca
     1441 gcaaatcgag aaatccattt ataaaacggt cgaggccaaa ttttgtaaca ccgtatttac
     1501 gtgcctgatg ttgtacttct ttttcaccaa tttttgcaaa gccctgccac ttggcaatta
     1561 ccggaatgta acgatgcatt tcgccataaa cttcaatatt ttttactact tcttttcggt
     1621 aagcttttag tccacaatta aaatcgtgaa ggttgtgtat gccactcatt atttttgttg
     1681 ccttgttgaa aagcttgcta ggtatggttt tcataatggg gtcgtagcgt ttacgtttcc
     1741 atcccgatac caaatcataa ccatcgttca ttatcatttt atacaatccg ggtatttcat
     1801 cggggctatc ctgcaggtcg gcatccattg ttataacaac atcgccctga gcaatttcaa
     1861 aacctttttg cagcgctgca ctcttaccat aattacgtct gaattttatg gcacgtactt
     1921 cggcattttg ttgtgctaaa ttttctatca cctgccacga tttatcttta ctgccatcgt
     1981 caataaaaat tacttcgtac gaatatttat ttgcttgcat cactttcgct atccacggaa
     2041 ataattcggg caacgattcg tcttcattaa aaagtggtat cactacagaa atatccatac
     2101 ttattctttt ggtgtaaaaa tactattatc ctttttataa atggctgacg ttatcaatac
     2161 tataacaaag ccgccaagaa ttttattaaa catatcgcct tgtgtaatgg agtaagccga
     2221 taaatattcg ctttgcgaaa tcatgctatc c
//
LOCUS       DAORQA010000291         2243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10825_length_2243_cov_5.260055, whole genome shotgun sequence.
ACCESSION   DAORQA010000291 DAORQA010000000
VERSION     DAORQA010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10825_length_2243_cov_5.260055"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>883)
                     /locus_tag="PK736_09190"
     CDS             complement(<1..>883)
                     /locus_tag="PK736_09190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HRC33600.1"
                     /translation="SFQTGRAHFAAKQYYVYGDRDELINLIEKNNPKLSSTYQHKGAT
                     PAVAFMFPGQGSQYVNMGKALYDDEIIFRQHADMCFDILKTQLQIDLKAILYPADGNE
                     AAAEENLKQTQYTQPALFVIGYSLAQLYMSWGIKPAKLIGHSIGEFVAACIAGVFSLD
                     DAIMLVGSRAKLMQAMPKGSMLSVRMPYQTVQPLLNKNCAVAAINGAELCVVSGPTED
                     IVSLQTELEKEEIACKLLHTSHAFHSPMMDPVLPDFEKVVRKVKLNAPQIPIVSTVNA
                     NILTDEQATDIQYWTKHL"
     assembly_gap    884..893
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<894..>2243)
                     /locus_tag="PK736_09195"
     CDS             complement(<894..>2243)
                     /locus_tag="PK736_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HRC33601.1"
                     /translation="VEQLWNNLVEGKESVTFFKNEELDPSVNDEQRNDPSYIKARGII
                     NGAENFDAAFFGINPKVADLTDPQHRILLQLSVEALERASINPNTYNGLIGIYAGTGN
                     NTYYQKNVVHYPKLIEQVGEFLTMTSNEKDYIATRIAYELNLKGPALSIHTACSTSLV
                     SIVTATDQLLEHKCDVAIAGGVAITSPIMSGYTYNEGSMNSADGHCRPFDKDARGTMF
                     SDGAGLVVLKRYKEAVRDGDTIYAVIRGGALNNDGGGKASFTAPSVDGQAQVIALAHA
                     KANVNADTISYVEAHGTATPIGDPIEVEGLTKAFNLENKLVHTSGLGSIKGNFGHLTA
                     AAGVAGLIKVVLQLQHKKLVPTINYQSPNPQIKFENTPFHVIDKLQDWTNENNPLRAG
                     VSSFGVGGTNAHLILEEAPKPQPSGIARPVHTLMLSAKNNEALQRYKTKVLTELKSNE
                     "
BASE COUNT          608 a          481 c          429 g          715 t
ORIGIN      
        1 taaatgtttt gtccagtatt gaatatcggt cgcctgttca tcggttaaaa tatttgcatt
       61 cactgtcgat acaattggaa tttgcggtgc attcaacttt acttttcgta ctactttttc
      121 aaaatcggga agcacaggat ccatcattgg agagtgaaac gcatgtgatg tatgcaatag
      181 tttgcaggca atctcttctt tttctaattc tgtttgtaac gaaacaatat cttcagtagg
      241 gcccgacacc acacataatt ccgcaccatt aattgcagca acggcacaat ttttattcag
      301 taaaggttgc acagtttggt aaggcatacg caccgacaac atacttccct ttggcatggc
      361 ttgcattaat tttgcacgcg aacctacaag cataattgca tcatcgagtg aaaacactcc
      421 ggcaatgcaa gccgcaacaa actcgccaat gctgtgtcct ataagtttag ccggttttat
      481 tccccaactc atatataact gtgcaagcga gtatcctatc acaaaaagtg caggttgtgt
      541 gtattgtgtt tgttttaagt tttcctcggc tgcagcttcg tttccatcgg caggataaag
      601 aatggctttt aaatctattt gcaattgtgt ttttaaaata tcgaaacaca tatcagcatg
      661 ttggcgaaaa ataatttcat cgtcatacaa tgccttgccc atatttacat attgcgaacc
      721 ctgccccgga aacataaatg ccacagccgg agttgcacct ttgtgctgat aggtagacga
      781 taatttagga ttattttttt cgattaaatt tataagctca tcgcggtcac cgtatacgta
      841 gtattgtttt gcagcaaaat gggcgcgccc tgtttgaaac gaannnnnnn nnnttcgttg
      901 cttttcaatt ctgtcagcac ttttgtttta taccgttgta atgcttcatt attttttgcc
      961 gatagcatta aagtatgaac cggccttgca atgcccgatg gctgaggctt tggtgcttct
     1021 tctaaaatta aatgtgcatt ggtgccacct acaccaaacg atgatactcc ggcacgtaaa
     1081 ggattatttt cgttggtcca gtcctgcaat ttatcaataa catgaaatgg agtattttca
     1141 aattttatct gcggattggg cgattggtaa ttgatggttg gcaccaattt tttatgttgc
     1201 aattgcagca ctacttttat cagcccggcc acaccggcag cagcagtaag gtgtccaaaa
     1261 tttcctttta ttgaacccaa tcctgatgta tgcaccaatt tattttcaag attaaatgcc
     1321 tttgttagac cttcaacttc tataggatcg ccaatgggag ttgccgtacc atgtgcttct
     1381 acataactta tagtatcggc atttacattt gctttggcat gtgccaatgc aattacctgt
     1441 gcttgtccat ccacactggg cgcggtaaaa cttgctttgc caccaccatc attattcaat
     1501 gcaccaccgc gtattacagc ataaatagta tcgccatcgc gtactgcttc tttatatctt
     1561 tttaaaacaa caagtccggc accatcgcta aacatggtac cacgggcatc tttatcgaaa
     1621 gggcggcaat gcccatcggc actattcatg cttccttcgt tgtacgtata tccactcatg
     1681 atgggcgatg tgattgcaac accaccggca atggcaacat cgcatttatg ctcgagtaat
     1741 tgatcggtag cagtgacaat agaaacaagt gaggtgctgc aggcagtgtg aatgctcagg
     1801 gcagggcctt tcaaatttaa ttcgtaagca atgcgtgtag cgatataatc tttttcgttt
     1861 gatgtcatgg taagaaactc gccaacctgt tcaattaact ttgggtaatg cactacgttc
     1921 ttttggtagt aggtattgtt tccggttccg gcataaatac ctatcaaacc attataagta
     1981 tttggattta ttgatgcacg ctccaaagct tctaccgata actgtaatag tatacgatgt
     2041 tgcggatcgg taaggtcggc tactttagga ttgattccaa aaaacgcagc atcgaaattt
     2101 tcggcaccat taattattcc acgtgcttta atataagaag gatcatttct ttgctcgtca
     2161 ttcacagaag gatctaattc ttcattctta aaaaatgtaa cactctcttt gccctccact
     2221 aaattattcc aaagttgttc aac
//
LOCUS       DAORQA010000292         2226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10908_length_2226_cov_11.824965, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000292 DAORQA010000000
VERSION     DAORQA010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10908_length_2226_cov_11.824965"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..1839
                     /gene="typA"
                     /locus_tag="PK736_09200"
     CDS             28..1839
                     /gene="typA"
                     /locus_tag="PK736_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRC33602.1"
                     /translation="MQSIRNIAIIAHVDHGKTTLVDKILHQTNIFRDNQASGELILDN
                     NDLERERGITITAKNVSVTYKGVKINIIDTPGHADFGGEVERVMSMADGVLLLVDAFE
                     GPMPQTRFVTEKALTAGLRPILVINKVDKENCRPDEVHEAVFDLFFNLGATQEQLDFP
                     TLYGSSKQGWMNTDWKVKTDNITPLLDAILEHVPEAPRNEGTPQLLISSLDYSSFVGR
                     IAIGRLLRGELKENMPVSLCKRDGQITKTRIKELMTFEALGKKKIAHAYAGDIVALTG
                     IENFDIGDSVADFENPEPLIAVKVDEPTMSMLFTINNSPFFGKEGKFVTSRHLRDRLF
                     KEIEKNLAMRVEETASPDSYLVFGRGILHVSILIETMRREGYEFQVGQPQVLIKEIDG
                     VKCEPYETLSVDVPEESAGKVIELATMRKGDMMIMEPKGDLQHLEFEIPARGLIGLRN
                     QALTATQGRAVMNHRFKEYKPWRGDIPGRINGVLIAHEPGVTTPYSIDALQDRGVFFI
                     ESNENVYSGQIIGEQNKPGDLVVNVTRPKKLTNMRASGSDDNVRLTPKINMSLEENLE
                     YIQGDEMVEITPLSIRIRKLYLNHEDRKRYEKSGTAQ"
BASE COUNT          691 a          390 c          520 g          625 t
ORIGIN      
        1 cccgaaaaaa aaataaggga acagaatatg caaagtataa ggaatattgc tataatagca
       61 cacgttgacc atggcaaaac aacattggta gataaaatat tacatcaaac aaatattttt
      121 cgcgacaatc aggcttcggg cgagttgata ctggacaata acgacctgga acgcgaacgt
      181 ggaattacca taactgcaaa gaatgtaagt gtgacttaca agggtgtgaa gattaatatt
      241 atcgacacac cgggtcacgc ggactttggt ggcgaagtgg aacgtgtaat gagtatggcc
      301 gatggggtgc tgttgcttgt agatgctttt gaagggccta tgcctcaaac gcgttttgtg
      361 accgagaagg ctttgactgc agggctgcga ccaatattag ttatcaataa agtagataaa
      421 gaaaactgcc gccccgatga agtgcacgaa gcggtattcg atttgttttt taatttaggt
      481 gcgactcagg agcagttaga tttccctaca ttatacggct cgagcaaaca aggctggatg
      541 aataccgact ggaaagtgaa gacagataat attactccat tgcttgatgc tatattggag
      601 catgtgcccg aagcgccacg caatgaaggt actccacagc ttttaatttc ttcgttagat
      661 tactcatcgt ttgtgggtcg tattgccata ggccgattgt tgcgtggcga actaaaagag
      721 aacatgcctg tgagtttgtg taagcgcgat gggcaaataa cgaaaacgcg aattaaggaa
      781 ttgatgacct ttgaagcatt gggcaagaaa aaaattgcgc atgcttatgc aggcgatatt
      841 gtagcactta ccggtatcga gaattttgat attggcgatt cggtggcaga ttttgaaaac
      901 cccgaacccc ttattgccgt aaaggttgat gagcctacta tgagtatgtt gtttactata
      961 aacaactccc cattttttgg caaggaagga aagtttgtaa catcgcgtca cttgcgcgat
     1021 agattattta aggagataga aaaaaatctt gctatgcgtg tggaagaaac ggcatcgccc
     1081 gattcgtatt tggtgtttgg acgtggcata ttgcacgttt ctattttgat agaaaccatg
     1141 aggcgtgagg gctatgagtt tcaggttggc caaccacagg tattgataaa agaaattgat
     1201 ggtgtgaagt gcgaacctta cgagacttta agtgtagatg tgcccgaaga aagtgccgga
     1261 aaggttattg aacttgcaac tatgcgtaaa ggcgatatga tgataatgga gcctaaaggc
     1321 gacttgcaac atttggaatt tgagatacct gcacgaggcc tgataggttt acgtaatcag
     1381 gcgcttactg ccacacaggg ccgcgcggtt atgaatcatc gatttaagga atacaagcca
     1441 tggcgtggcg atataccggg ccgcataaat ggtgtgttga ttgctcacga accgggagtt
     1501 acaacaccct actcgataga tgccttgcaa gatcgtggtg tattttttat agagtcgaac
     1561 gagaatgtat acagtggaca aataataggt gagcaaaata aacctggcga cctggtggta
     1621 aatgttacac gcccgaaaaa attgaccaat atgcgcgcat cgggtagtga tgataatgtg
     1681 cgattgacac caaaaataaa catgtcgctc gaagaaaacc tcgagtatat acaaggtgat
     1741 gaaatggttg aaattactcc cctgtcaata cgcattcgca agttgtattt gaatcacgaa
     1801 gatagaaaga gatacgaaaa atcgggtact gcacaataaa tataatctgc ttttgaacgg
     1861 gcattgcaaa attgataatc attttcctgt ttcagtaatt taggttttat taagaaggca
     1921 atattatttt ttgtcttgat aaatattaat gcaataaatc atttttgatt attgcatttt
     1981 tttttgaatg tgaaatattg caaactcttt gaatattctg tgataaaacc aaacttgcat
     2041 agcagacgct accttaattt ctttaagtta agagaattgt ttttatcccg tagggattac
     2101 cgcttggtaa taaaccaaaa cactgtgaat tatgttccgg aggaactacc catttttcaa
     2161 tctttattta ccatcattgg gtagtcctta caggcatacg cgtcaacttt agctcgaatc
     2221 attcat
//
LOCUS       DAORQA010000293         2214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_10983_length_2214_cov_7.485873, whole genome shotgun sequence.
ACCESSION   DAORQA010000293 DAORQA010000000
VERSION     DAORQA010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10983_length_2214_cov_7.485873"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1887)
                     /locus_tag="PK736_09205"
     CDS             complement(<1..1887)
                     /locus_tag="PK736_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRC33603.1"
                     /translation="MHFSLSGQSNILLDSNLAYLQTITNDSLRVKTLLQIAKIYSNTD
                     PDSALFFLNKCESLSKSLAYKKNYNEALLLKANILTETNQFEKSEIVLNELLKIISND
                     AAQKKYERYVYNEYISYGRKTGNYKLALDYAFKLVKIAQTEKDEKLECSTLGNIAGIF
                     FDMNDLQKSLYYDQQTLALIVKNKDWKNYSLVNFNIGSVLGEMGEHDSALYYYNIADS
                     VALALNDKQMQAEINSNIAFEYFDAGDDANAKIFFDKAIKLYDELQIKDDSYAMLNCN
                     LGQLYMRQNDLAKAEQYLLRADELGNTSGFLSDKVEYKKQLALLYSKLKIWDKAFIYN
                     QQLMQLKDSLAKDENLRISHDLENKYNVAQKEKQILILNKDKQLQQKEIQRQRVLQYA
                     TLIIAAIFLLFGSLFLFQRIRIAKEKRKSDSLLLNILPKQTAEELKASGKTEAKYYES
                     CSILFTDFENFTTASELLSPQQLVSEIDMCYSAFDKILLKYNIEKIKTIGDSYMCVAG
                     LPAPNKNHAADILNAALEIRNFMNDLYEERKRKNEMCFKIRIGINTGPLVAGVVGSSK
                     FAYDVWGDAVNIASRMESSSETGKVNISGSTYELVKDNFACTYRGKIAVKNKGEIDMY
                     FAEKL"
BASE COUNT          675 a          417 c          344 g          778 t
ORIGIN      
        1 taatttctct gcaaagtaca tatcgatttc tcctttattc ttcacagcta ttttaccgcg
       61 gtaggtgcaa gcaaaattat cttttacaag ttcataagtg ctgccgctaa tatttacttt
      121 tcctgtttcg ctgctgcttt ccattcgcga tgcaatattc acagcatcgc cccacacatc
      181 ataagcaaac ttcgaactac ctacaacccc tgccaccagc ggccctgtgt tgataccaat
      241 gcgaatttta aaacacattt cgtttttcct ttttcgctct tcgtataagt cattcataaa
      301 attgcgaatc tctaacgctg catttaaaat atctgccgcg tggtttttat ttggtgcagg
      361 caagcctgcc acacacatat acgaatcgcc tattgttttt attttttcaa tattatactt
      421 caaaagtatt ttatcgaatg cactatagca catgtcaatt tctgatacca attgctgagg
      481 agaaagtaat tccgaagcgg tggtgaagtt ctcgaaatcg gtaaatagaa tcgaacatga
      541 ttcgtaatac ttagcttctg ttttgcccga tgcttttaat tcttcagcag tttgtttggg
      601 aagaatattg agcaataaac tatcactttt ccttttctct tttgcaattc gtatgcgttg
      661 aaataaaaac aaactgccaa acaaaagaaa aattgctgca atgataaggg tagcatattg
      721 caacactctt tggcgctgaa tttccttttg ctgcaattgc ttatccttat tcaaaattaa
      781 aatttgcttt tccttttgcg ccacattgta tttgttttcg aggtcgtgcg atatgcgcag
      841 gttttcatct tttgctaaag aatcttttaa ctgcattagt tgttgattat atataaatgc
      901 tttatcccaa atttttaatt tcgaatacaa caatgcaagc tgctttttgt attccacctt
      961 atccgacaaa aaaccggatg tgtttcccaa ttcatcggca cgcaacaaat attgctcggc
     1021 ttttgctaaa tcgttttgcc gcatgtataa ctgacctaaa ttgcaattga gcatggcata
     1081 cgaatcatct tttatttgaa gttcatcgta tagctttatg gccttatcaa aaaatatttt
     1141 tgcattggca tcgtcaccgg catcaaaata ttcgaaagca atattcgaat ttatttctgc
     1201 ctgcatttgc ttatcgttta gtgccagggc aacgctatca gcaatgttat aatagtacaa
     1261 tgcgctatcg tgctcaccca tttcgcccaa caccgaacca atgttgaaat ttactaatga
     1321 ataatttttc cagtctttat tttttacaat caatgccagc gtttgctgat cataatacag
     1381 cgacttttga aggtcattca tatcgaaaaa tattccggca atatttccaa gtgttgaaca
     1441 ttctaatttc tcatcctttt ctgtttgagc aatcttcaca agtttaaaag catagtcaag
     1501 cgccagctta taatttcctg ttttgcgacc atatgatatg tactcattat aaacataacg
     1561 ttcgtatttt ttttgtgctg catcgttcga aataattttc aataattcat tcaatactat
     1621 ttccgatttc tcaaattggt tagtttcggt gaggatattt gcctttagca gcaaagcctc
     1681 attataattt tttttgtaag cgagcgattt tgacaatgat tcgcatttgt ttaaaaaaaa
     1741 taaagccgaa tcgggatctg tgttgctgta tatttttgca atctgtaata aagttttaac
     1801 ccttaacgaa tcgttggtta tagtttgcaa atatgccaaa ttagaatcga ggagtatatt
     1861 actttgtcct gataaactga aatgcattat gaatgccatc gccaaaatcg gccatgtttt
     1921 catttgtaaa aagttgatgg acaagacacg tagcattagt attttatgat taatacaatg
     1981 gcacaatgta gaaaattgtt tttcaaaaat gcgatttgaa ttgtgcgatt tgatatttcc
     2041 gaaaattaca ttttcgtaaa tcgcttaaaa gatgaatgcg atgagtgatt tacagtaaca
     2101 atatacgaac cagcattcca atttgaaaca gaaattgatt ttataattac gtcagcataa
     2161 ccatgccaaa aaaattactt aattaaattt ataaaaaaaa aaaaaaaaaa aaaa
//
LOCUS       DAORQA010000294         2195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11065_length_2195_cov_8.716822, whole genome shotgun sequence.
ACCESSION   DAORQA010000294 DAORQA010000000
VERSION     DAORQA010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11065_length_2195_cov_8.716822"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PK736_09210"
     CDS             complement(<1..784)
                     /locus_tag="PK736_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC33604.1"
                     /translation="MSKNYATDVEAVDDLKKSYHRLRDEIGKVIIGQDEIVRDVLISI
                     FTNGHCLLVGVPGLAKTLLVNTIARVLGLTYNRIQFTPDLMPSDIIGTEILNDQRHFE
                     FIKGPLFANIILADEINRTPPKTQAALLEAMQERAVTTAGKRYELASPFFVLATQNPI
                     EQEGTYPLPEAQLDRFMFNVMLTYPTFDEEVTVVKNTTSSYNPTLDKILDAEEILYFQ
                     QLIKRIPIPDNVLNYAVSLASKTRSGTKTAADISNQYIAWGAG"
     gene            complement(951..>2195)
                     /locus_tag="PK736_09215"
     CDS             complement(951..>2195)
                     /locus_tag="PK736_09215"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRC33605.1"
                     /translation="LKSNIACIALVCITCIGFAQPKTVDKIIGVAGNKIILLSELEQQ
                     YAQYIAQGNYANPAMRSQIFNQLMTNKMLLYHAMLDSVEVSESQVEEQLDRRFAYFIG
                     QFGGEKELEKYYNKSIIELKEEFRPTIKDQLLVQQMQSKVTKNSSVTPEDVRNYFESI
                     PKDSLPLINTEVEYAQITATVAYNEESKTTCKAKLEEYRQRVLKGEDFGALAVLYSLD
                     KGSAKNSGELGFMNRGDLVPEYEAAAFKLKPGEVSAIVESKFGYHIIQMIERRGEKFN
                     TRHILLRPQITDDDNIRVANFLDSIAALIKNDSMTFSEAAIKFSDDNETRFNGGKIVN
                     PQSGSTRFETDQVEPSTFFQLDQLQPGQMTKVLTYTAADGKTGYRILMLLNRTVPHVA
                     NLKDDYQRLQGVASSEKETKAL"
BASE COUNT          581 a          439 c          439 g          736 t
ORIGIN      
        1 gtcctgcacc ccatgcaata tattggttcg aaatatcggc agcagttttt gttcccgagc
       61 gggtttttga tgctaatgaa acagcgtagt taagcacatt atcaggaata ggaatccttt
      121 ttatcaattg ctgaaaatag agaatctctt cggcatccaa tattttatct aatgttggat
      181 tgtatgatga tgtagtgttt tttaccacag taacttcctc gtcaaaggtt gggtaagtca
      241 acattacatt aaacataaag cggtcgagtt gtgcttctgg caaaggatag gttccctcct
      301 gctctatcgg attttgtgtt gctaaaacaa agaaaggcga ggcaagttcg tagcgcttgc
      361 cggcagtagt tacggcacgt tcctgcatgg cttcgagcag tgccgcttgt gttttgggag
      421 gagtacggtt tatttcatcg gccagaatta tattggcaaa caaggggcct tttataaatt
      481 caaaatgccg ttggtcattc agtatttcgg taccaataat atcgcttggc ataaggtcgg
      541 gcgtaaattg aatacggttg tacgttaatc ccaacacccg tgctatggta tttaccagca
      601 atgtttttgc aaggcccggc acacccacga gcaaacagtg tccattagta aaaatagaaa
      661 taagcacatc gcgtactatt tcatcctggc caataattac tttacctatt tcgtcacgta
      721 atcgatgata ggattttttt aaatcgtcta cagcttccac atcggtggca taattcttac
      781 tcataaaaat attttatctt aaaaaatgtt gcaaattaaa tcaattgaag tgatgaaaaa
      841 ccaaagattt ggttaaccta tttgtttagc cattttgcac aggcgctatt atttttaaat
      901 tcgtcataca tgagaaagta tgttgagcct tttttacgag ctatccattc ttacaaagct
      961 ttggtttctt tttcggatga agccacgcct tgcagccgtt gatagtcgtc tttcaggttt
     1021 gctacatgtg gcacagttct gttgagcagc atcaaaattc tataaccagt ttttccatct
     1081 gctgctgtat aggttaaaac ctttgtcatt tgtccgggct gtaactgatc gagttgaaag
     1141 aaagtagaag gttctacctg atcggtctcg aagcgtgtac tgccactttg gggattcact
     1201 atttttccac cgttaaatct tgtttcgtta tcgtccgaaa attttattgc tgcttcacta
     1261 aaagtcatcg agtcgttttt tatcagggcg gcaatactat caaggaaatt ggccacacga
     1321 atattgtcgt catcggttat ttgtgggcgc aataatatgt ggcgggtatt aaatttctcg
     1381 cctctgcgtt ctatcatttg aataatgtgg taaccaaatt tcgactcaac tattgccgaa
     1441 acctcaccgg gcttaagttt gaatgcggca gcttcgtatt cgggcaccag gtcaccacga
     1501 ttcataaagc ccagttcgcc actatttttt gctgatcctt tgtcgaggct gtataacact
     1561 gcaagtgccc caaagtcttc gcctttcaac acccgttgac ggtattcttc gagcttggct
     1621 ttgcatgttg ttttggattc ttcgttataa gccaccgtag ctgtaatttg tgcatattca
     1681 acttcggtat ttattaaagg taaactatct tttggaatcg actcaaaata attgcgcacg
     1741 tcttcgggag ttacagagga attttttgtc actttacttt gcatctgttg caccaatagc
     1801 tgatctttta tagtgggtcg aaattcttct ttaagttcga taattgattt gttgtaatat
     1861 ttttctaatt ccttttcgcc accaaattgt ccaataaaat aggcaaacct gcggtcgagt
     1921 tgctcttcca cctgcgattc cgatacttca acactatcga gcatggcatg ataaagcaac
     1981 attttattgg tcatcagttg attaaagatt tgacttcgca tggcagggtt agcataattt
     2041 ccctgagcta tatactgtgc atattgttgc tcgagttcgc tcaataaaat tattttattt
     2101 cctgcaacac ctattatttt atcaacggtt ttgggttgag caaaacctat gcaggtaata
     2161 caaaccaatg cgatgcaggc aatgttactt tttaa
//
LOCUS       DAORQA010000295         2195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11068_length_2195_cov_7.884112, whole genome shotgun sequence.
ACCESSION   DAORQA010000295 DAORQA010000000
VERSION     DAORQA010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11068_length_2195_cov_7.884112"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..>918
                     /locus_tag="PK736_09220"
     CDS             58..>918
                     /locus_tag="PK736_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRC33606.1"
                     /translation="MKSYDEKHIKNVVLIGAAKTGKTTLCETMLYEAKLIQRRGSVEE
                     KNTVSDYHDIEHERGGSVYATAMHTEWRDYKINIIDTPGLDDFMGEVASSIRVCDTAI
                     MLLNAQHGVEIGTELIWDYCDKYRKPTLFAINQCDHEKADFWNAVEEAKNSFGKAVTV
                     MQYPVNQGAGFNQIIDLLKMVMYQFGKDGGKPEKLAIPESELERANELHNALVEKAAE
                     NDEKLMELFFEKGNLDEDELRQGIKLGMMHHDVFPVFCLSAKRDMGSGRLMGFIDNVA
                     PSAIEMPPEIT"
     assembly_gap    919..928
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <929..>2195
                     /locus_tag="PK736_09225"
     CDS             <929..>2195
                     /locus_tag="PK736_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRC33607.1"
                     /translation="DGQTVACDPSKPASVFVFKTLIEPHLGRLSFFKVTSGEVKAGMD
                     FTNSTTDSVEKFTQLFIMDGKNRNPIDKLSAGDIGATLKLKNTSTNDTLCAKGASFSI
                     APIEFPQSRVRVALETKNKNDDERISTVLHEIHAQDPTLIVQYSPELKQIILQAQGDL
                     HLTVTKWRLHHIYKLEVEFTKARIPYRETIHKMALATYRHKKQSGGNGQFGEVHIRIE
                     PYYEGMPPLKEYPVRDTEEHKLDWGGKLVYNNCITGGVIDTRFMPSILKGVMEKMTEG
                     PITGSHVRDIRVSVFDGKMHAVDSNDLSFKIAGMMAFKEAFHNAEPQLLEPVHNVEIA
                     VPEKMLGDVMTELTTRRSTIMGMDSDNNIQIIKAQTPLAEIDKLAASLRSITQGKGKM
                     KNSFFDYAPVSSDMQKKLHEEYLKVAVDN"
BASE COUNT          726 a          407 c          481 g          571 t
ORIGIN      
        1 gtaatagtta ggcaaatttg tgtatcttaa tttctaacaa tttaaaattt atgatgtatg
       61 aaatcttatg acgaaaaaca cattaaaaac gtagtgctta ttggtgccgc taaaaccggt
      121 aagactacgt tatgcgaaac catgctctac gaggctaagc taatacaaag gcggggcagc
      181 gtagaggaaa aaaatactgt gagcgattat cacgatattg aacacgaacg tggcgggagt
      241 gtatatgcta ctgccatgca taccgaatgg cgcgattata aaataaatat tattgacacc
      301 cccggactcg atgattttat gggtgaagta gcatcttcta ttcgtgtttg cgatactgcc
      361 ataatgttac ttaatgcaca acatggtgtt gaaataggta ctgagttaat ttgggactat
      421 tgtgataaat accgtaagcc aacattgttt gccataaacc aatgcgacca cgagaaggcc
      481 gatttttgga atgcagtaga ggaagcaaaa aatagttttg gcaaagcggt aactgtaatg
      541 cagtatcctg ttaatcaagg tgcaggtttc aatcaaatta tcgatttgct aaaaatggtg
      601 atgtatcaat ttggcaaaga tggtggtaag cccgaaaaac tggcaattcc cgaaagcgag
      661 ttagagcgtg ccaatgaatt gcataatgca ctggtagaga aagcagctga gaacgatgag
      721 aagctaatgg aattattttt cgaaaaagga aatcttgacg aagatgaatt gcggcaagga
      781 ataaaattgg gcatgatgca ccacgatgta tttcctgttt tttgtttatc ggcaaaacgc
      841 gacatgggtt cggggcgact aatgggcttt atagataatg ttgcgccatc ggccattgag
      901 atgccacccg aaataaccnn nnnnnnnnat gacggacaaa ctgttgcctg cgatccttcg
      961 aaacctgcat cggtatttgt tttcaaaacg ttgatagaac cgcaccttgg ccgcttatca
     1021 ttttttaaag ttacaagtgg cgaagtaaaa gccggtatgg attttacaaa ctctacaacc
     1081 gatagtgtgg agaaatttac acaacttttc attatggatg gaaaaaaccg caaccctatt
     1141 gataagttga gtgcaggtga tattggtgca actttaaaat taaaaaacac ttcgaccaac
     1201 gatacgctct gtgcaaaagg ggcttcgttt tcaattgcac ccatcgagtt tcctcaatcg
     1261 cgtgtgcgtg ttgcattaga aacaaaaaat aaaaatgatg atgagcgcat cagcactgtg
     1321 cttcacgaaa tacatgcaca agacccaaca ctcatagtgc aatattcacc cgaactgaaa
     1381 caaattattt tacaagctca aggcgatttg catttgacag taactaaatg gcgattacac
     1441 catatttata aacttgaagt ggaatttaca aaagctcgca taccttatcg cgaaactatt
     1501 cataaaatgg cattggctac ttaccgccac aaaaagcaat cgggtggcaa cgggcaattt
     1561 ggcgaagtgc acatccgcat tgagccatac tacgaaggta tgccaccact caaagaatat
     1621 cccgtgcgcg ataccgaaga acataaattg gactggggcg gaaagttggt gtacaacaat
     1681 tgtattactg gcggagtaat cgacacgcgc tttatgcctt cgatacttaa aggtgtaatg
     1741 gagaaaatga ctgaaggccc tatcaccggt tcgcatgtgc gcgatatacg cgtgagtgtg
     1801 ttcgatggaa aaatgcatgc agtagattcc aacgatttat cattcaaaat tgccggcatg
     1861 atggccttta aagaagcgtt tcacaatgcc gaaccacaat tgcttgaacc tgttcacaat
     1921 gtggaaattg cagtgcccga aaaaatgttg ggcgatgtaa tgacagaact aactacacgc
     1981 agaagcacta ttatgggaat ggatagtgac aataatatac agataataaa agcgcaaaca
     2041 ccgcttgccg aaatagataa acttgccgca tcgttgcgaa gtattacgca aggcaaaggc
     2101 aaaatgaaaa atagtttttt tgattatgct ccggtatctt ccgatatgca gaaaaaactt
     2161 cacgaagaat atttaaaagt tgctgtagat aatca
//
LOCUS       DAORQA010000296         2194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11074_length_2194_cov_11.560542, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000296 DAORQA010000000
VERSION     DAORQA010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11074_length_2194_cov_11.560542"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="PK736_09230"
     CDS             <1..1056
                     /locus_tag="PK736_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33608.1"
                     /translation="TSDTAELCNNMVYLNGHFYLAGAYYYKLNNGGQTGPLPLLYDID
                     SQGNLIKTFYEDTFGLISSIGFDSYNNLLMGADIYYPYFQLYKYTLNSGLAVLDTCYI
                     SETGNNNYQINLGQNKNASVFDGINNSFTHTQTKIQIIDEQCQNTFKLDFGNPNEDSR
                     CAYFGAIDTISKNRIIVGGTANFIYGTSLYMPVQSQFHCAVFDDSLNVIWESHAGDDA
                     YHVMMRAKPTLDGGVVCVGYRYDYLNNFPTENRDPIVMKFDSLGNIQGSYDNNNQYQS
                     MFNVYPNPASDILNIEALDNLEYDYEVYNITGDRLMQGELKSHTKINILKFSSGVYYL
                     KIVSKKGSSFKKFLVTH"
     gene            complement(1046..2146)
                     /gene="ychF"
                     /locus_tag="PK736_09235"
     CDS             complement(1046..2146)
                     /gene="ychF"
                     /locus_tag="PK736_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRC33609.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNCLSNAKAVAANFPFCTIEPNIGVI
                     TVPDERLNELERLVKPQRVVPTTIEIVDIAGLVKGASKGEGLGNQFLANIRETDAIIH
                     VLRCFENDNIIHVDGSINPIRDKEIIDTELQLKDLETVEKKIQRIEKVAKAGNDKEAK
                     KGYDVLCTYKSHLESGQSARSAPVAIEDKKYLSDCFLLTDKPVLYVCNVDEQALKAGN
                     KFTEQVREAVKNENAEVLIIAAAIEADIAQLESYDDRVLFLSDLGLAESGVSLLIKAA
                     YRLLDLYTYFTAGEKEVRAWTIHKGMTAPQAAGVIHTDFERGFIRAEVIAYENFVKFG
                     SESACRDNGKLSIEGKEYIVKDGDLMHFRFNV"
BASE COUNT          676 a          410 c          404 g          704 t
ORIGIN      
        1 acaagtgata cggctgagct ttgtaacaac atggtttatt tgaatggaca tttttatctg
       61 gcgggtgcat actattacaa attaaataat ggcgggcaaa ccggcccact accattattg
      121 tatgatattg atagccaagg aaatttgata aaaacatttt acgaagatac atttggttta
      181 ataagttcta tcggttttga ctcctacaat aacttgttga tgggtgctga tatttattat
      241 ccatattttc aactttataa atatactttg aacagtggct tagctgtttt agatacttgt
      301 tacatatcag aaaccggcaa caataactat caaattaatt tagggcaaaa taaaaatgca
      361 agcgtatttg atggtataaa taatagtttt actcatacac aaacaaaaat tcaaattatt
      421 gatgagcagt gtcaaaatac atttaagttg gatttcggga acccaaatga ggactcccgt
      481 tgtgcttatt ttggagcaat tgacacaatt tctaaaaata gaattattgt tggcggcacc
      541 gcaaatttta tttatggtac atctttgtat atgcctgtac aaagccaatt tcattgtgct
      601 gtttttgatg atagtttgaa tgttatttgg gagtcacatg ccggagacga tgcttatcat
      661 gtaatgatga gggcaaagcc aacattagat gggggtgtag tttgtgtagg atatagatac
      721 gattatctaa ataatttccc aacagaaaac cgcgacccca tagtaatgaa attcgattcg
      781 cttggaaata ttcaaggaag ctatgacaat aataatcagt atcaaagtat gttcaacgta
      841 tatccaaatc ctgcaagtga cattttaaat attgaggcac tcgataattt ggaatatgat
      901 tatgaggtgt acaatattac cggagataga ctgatgcagg gggaattgaa atcacacaca
      961 aagattaata ttttgaaatt tagtagcgga gtttattatt tgaaaattgt ttcaaagaaa
     1021 ggaagttcat tcaaaaaatt tctcgttaca cattaaatcg aaagtgcatt aagtcgccat
     1081 cttttacaat atattcttta ccttctatag ataattttcc attatcgcgg caagccgatt
     1141 cggagccaaa ctttacaaaa ttttcgtaag caattacttc ggcacgaata aatccacgtt
     1201 cgaaatcggt gtggataaca cccgcagcct gcggtgctgt catgcctttg tggatagtcc
     1261 atgcgcgcac ttctttttcg cctgcggtaa aataagtgta taaatcaagc aagcgataag
     1321 cagcttttat caataaactt acaccacttt cggctaatcc caggtcactt aaaaataaaa
     1381 cgcggtcgtc ataactttcg agttgggcaa tatcggcttc tatagctgcg gctataatta
     1441 gcacttcggc attttcattt tttactgcct cgcgtacctg ctcggtaaat ttatttcctg
     1501 ctttgagggc ttgttcatct acattgcaaa catacaatac gggcttgtcg gtgagtaaaa
     1561 agcaatcgga caaatatttt ttatcttcaa tggccacagg tgcagagcgt gccgattgtc
     1621 cgctttcgag atgcgattta taagtgcaca aaacatcgta accttttttc gcttctttat
     1681 cgttaccggc tttggcaact ttttcaatgc gttgaatttt cttttctacc gtttccaaat
     1741 ctttcaattg caattcggta tcgataattt ctttatcacg aattggatta atgctgccat
     1801 ccacatgaat aatattatcg ttttcgaaac accgcaacac atgaatgatg gcatccgttt
     1861 cgcgtatgtt agccaaaaac tgattgccca atccttcgcc cttactggca ccttttacca
     1921 gcccggcaat gtcaacaatt tcaatagttg tgggcaccac acgttgtggc ttaacgagac
     1981 gttccagttc attgaggcgc tcatcgggca ctgtaattac accaatattg ggctctattg
     2041 tacaaaaagg aaaatttgcc gcaaccgcct tggcattcga caaacaattg aagagggttg
     2101 atttacctac gtttggcaat cctactatac cacattttaa actcataaac taatattatt
     2161 tttttgaaaa acggctgcaa atatagtatt gcca
//
LOCUS       DAORQA010000297         2183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11141_length_2183_cov_5.577538, whole genome shotgun sequence.
ACCESSION   DAORQA010000297 DAORQA010000000
VERSION     DAORQA010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11141_length_2183_cov_5.577538"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1955
                     /locus_tag="PK736_09240"
     CDS             <1..1955
                     /locus_tag="PK736_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33610.1"
                     /translation="IEQSTYTNIEGNYSFDTDTGLYTIEVDTANLPFGVVCPSTFYHT
                     AYIAKNDTTSENKNFAMGCKPGFDVGTTTIVRDSGIFRPANFANVRILAGDMSNHYGL
                     HCAAGTSGAVQVIITGPATYIGTVNGALTPIVNGDTLTYTIADFGLVDFDNDFQIQVQ
                     TDTLAQLGQQICFDVIVTPILGDNNPANNSLAHCFTVVNSYDPNLKSVYPINRIDNAD
                     GKLTYTIQFQNTGNAPAQHIYIMDTLDAALDASTFELLAYSVQPQVQLIGNNLRFNFP
                     NINLPDSTNDEPNSHGYVQFSIKLKDSLPLGTTINNTSFIYFDFNPPVQTNAVTDTVT
                     DCDNLIANAWFAKTSLCKNDTLFAAMELQYPYNNIKWLVDNVLAATGDSVSISNLSLG
                     THIIKVVIKTPYCLSKTYCTITVNDLPQPSLGNDTTACAGLVLDGGAGFVNYLWNNGA
                     TTQTIIANNSATYTVTVTDANGCKASDVLVALINPNPQILFNAFAIDTLCKNAGIQTI
                     AAPSPIGGTLTGNGLIGLNFDPTAVANGWNYFMYTYTDSNSCSKTAIDSVWVDDCLGI
                     NSANGNVYFMIAPNPTTDFITITTNLQSNNCKAMLYDMLGKELLSRKINSNTTQLNLQ
                     RLSKGNYLLKVFDGDALVGVERVVKM"
     gene            complement(1971..>2183)
                     /locus_tag="PK736_09245"
     CDS             complement(1971..>2183)
                     /locus_tag="PK736_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRC33611.1"
                     /translation="GYKIQLTFNDKKTRVVDFEKFLKTRPHPQYNKYNELHHFKKFKI
                     EWGNIVWGKDWDLIFPVDQLYKGKIH"
BASE COUNT          693 a          429 c          408 g          653 t
ORIGIN      
        1 atattgaaca atccacatat acgaatattg aaggaaatta ttcgttcgat actgatacag
       61 gtttatatac cattgaagtg gatactgcaa atttgccatt tggagtagtt tgtccatcca
      121 ccttttacca taccgcctac attgcaaaaa atgacaccac aagcgaaaat aaaaattttg
      181 caatgggatg caaacccggt ttcgatgttg gcaccacaac tattgtgcgc gactccggca
      241 tcttccgccc cgccaacttt gccaatgtaa gaatacttgc aggtgatatg agtaatcatt
      301 acggcttgca ctgtgccgca ggcacaagcg gtgctgtgca agtaataatt accggcccgg
      361 caacgtatat aggcactgtt aatggtgcgc tcaccccaat tgtaaatggt gatacgctaa
      421 cctacaccat tgcggatttt ggtttggtag attttgataa tgattttcaa atacaagtgc
      481 aaacggatac acttgcgcag ctcgggcaac aaatttgttt tgatgttatt gtgacgccaa
      541 ttttaggaga taataatcct gcaaataatt ctcttgcaca ttgctttaca gttgtaaata
      601 gttatgaccc aaatttaaaa tcggtttatc caattaatag aattgataat gctgatggca
      661 aattaacata cactatacaa tttcaaaata cgggtaatgc gcctgcccaa catatatata
      721 ttatggatac gcttgatgcc gcgcttgatg caagcacgtt cgagttgttg gcatattcgg
      781 tgcaaccaca agtgcagttg ataggaaata atttacgttt caattttcca aatatcaatt
      841 tgccagatag cacaaacgat gaacccaata gtcatggtta tgtgcagttt agtataaagt
      901 tgaaagatag tttgccactt ggcacaacaa taaataatac cagttttatt tatttcgatt
      961 ttaatcctcc ggtacaaact aatgccgtaa ccgatactgt aaccgattgc gataatctga
     1021 ttgccaatgc atggtttgca aaaacttcgc tttgcaaaaa cgatacacta tttgctgcca
     1081 tggaattgca gtacccctac aacaatataa aatggttagt tgataatgta cttgcagcaa
     1141 cgggagattc tgtttctatt tcaaatttaa gtcttggcac gcacattata aaagtggtaa
     1201 taaaaacacc ctactgttta tcgaaaacat attgtaccat cactgtaaac gatttaccgc
     1261 agccatcgct cggcaacgat acaacagcat gtgcgggatt ggttttagat ggcggtgcgg
     1321 gttttgtaaa ttatttatgg aacaatggcg caactacaca aacaataatt gcaaacaaca
     1381 gcgcaacgta tactgttaca gttactgatg ccaatggatg taaagcaagc gatgtgttgg
     1441 tggcattaat aaacccgaat ccacaaatat tattcaatgc atttgcaata gatacgcttt
     1501 gtaaaaatgc cggcatacaa accatagccg ccccaagccc aattggcggc acactcaccg
     1561 gcaacggatt aataggcttg aacttcgacc ctaccgcagt tgccaatggc tggaattatt
     1621 ttatgtacac ctacacagat agcaacagct gcagcaagac tgccatagat agtgtatggg
     1681 tagatgattg cctcggcata aattctgcaa atggaaatgt ctattttatg atagccccaa
     1741 acccaaccac cgattttatc accatcacca caaatttgca aagcaataat tgcaaagcaa
     1801 tgttatatga tatgcttggc aaagaattgc tttcacgcaa aataaattca aatactactc
     1861 aactaaattt gcaaaggctg agcaagggga attatttgct taaagtattt gatggtgatg
     1921 ctttggtggg agttgagcgg gtggtgaaga tgtgaacaat gaaatttgtt ttaatgaatt
     1981 ttgcctttat ataattggtc tacaggaaaa atcaaatccc aatcttttcc ccatacaatg
     2041 ttcccccatt cgattttaaa tttcttaaaa tggtgcaatt cattatactt gttatattgt
     2101 ggatgtggtc ttgtttttag aaacttttca aaatctacaa ctcttgtttt cttgtcatta
     2161 aaagttaatt gtatcttata ccc
//
LOCUS       DAORQA010000298         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11277_length_2159_cov_13.924905, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000298 DAORQA010000000
VERSION     DAORQA010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11277_length_2159_cov_13.924905"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..725)
                     /gene="aat"
                     /locus_tag="PK736_09250"
     CDS             complement(120..725)
                     /gene="aat"
                     /locus_tag="PK736_09250"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRC33612.1"
                     /translation="MRQFPKLTNDLKECAIVRTFELSEKLIMDGYSRGFFPWYEEEGC
                     FYWFAYNPRFVIFPQKIKVSKSMRQLIHKNKYRITFNTAFEKVMMKCALTERGDDNHT
                     WINQSFLDMYCDLHSKGICKSVEVWNGDELVGGLYGTVINNVFCGESMFHTEPNTSKL
                     ALIWLCQNKNFTLIDCQVETQHLISMGAEMIDLETYLAFVG"
     gene            complement(869..1963)
                     /locus_tag="PK736_09255"
     CDS             complement(869..1963)
                     /locus_tag="PK736_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33613.1"
                     /translation="MKADNSNSEILNHLSADEMLALQAGNMKGEQAQRCRNHIALCAL
                     CSEAMEGVSLMPFPIHIKDIKTQWTARTSKSLKHKPLKPTHNLPVVVIITALLIFCCA
                     YFWYFINQRQKIKSANPTNVSEQIIKPQGEKFVAPTFTTIENKNETIITYLEYNAAKK
                     DVHKHIESISPLSAVNPARIEISDATIPDLKNMDATYNNIIYMSGLKTIDYTNLYSDE
                     ESAKINSGLQAQFKNRDNGNAKNDILANTNTVDYRILLARAMQAIHENENTNAIAMFD
                     DILVVKPMDANCIFYKGIALENLNDHSNAILWFDKIIEMQTNPFLEEATFHKAVCLWN
                     TDNKSAAKELFLQIVESKTFYSNQAAAYLK"
     gene            complement(1950..>2159)
                     /locus_tag="PK736_09260"
     CDS             complement(1950..>2159)
                     /locus_tag="PK736_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRC33614.1"
                     /translation="YAHLPTLPEAIESINEQQRICIQLFYLEKKSYMEISTQTGYDLN
                     QVKSFIQNGKRNLKNYLLNKKNESR"
BASE COUNT          627 a          407 c          378 g          747 t
ORIGIN      
        1 acaacaaatc tctataccca atacaaaata cccaatacaa caagtctcaa tactcactac
       61 tataaatctc aatactcaat acaaaaatac tcaatacaaa aatacccaat acaaaagcac
      121 taacccacaa atgccaaata cgtttcgagg tcaatcatct ctgcacccat cgaaattaaa
      181 tgttgtgttt ctacctgaca atcgataagg gtaaaatttt tattttgaca caaccatatc
      241 aacgcaagtt tcgaagtatt gggttcggta tgaaacatac tttcgccaca aaaaacattg
      301 ttgattacag tgccatataa accaccaacg agttcatcgc cattccacac ttcaaccgac
      361 ttacaaattc ccttgctgtg taaatcgcaa tacatatcga gaaaagattg gtttatccag
      421 gtgtggttat catcaccacg ttcggtaagt gcacatttca tcatcacctt ttcgaaggca
      481 gtattaaaag taatgcgata tttattttta tggataagct gccgcatcga tttcgacact
      541 tttatttttt gcggaaaaat tacaaaacgt gggttgtatg caaaccagta aaaacagcct
      601 tcctcttcgt accaaggaaa aaacccgcgc gaatacccgt ccattatcaa tttttcggaa
      661 agctcaaacg tacgcacaat agcgcattct ttgaggtcgt tggtaagttt gggaaactgc
      721 cgcattcaat tatttatttg cgtttcaaat agtgcaatgc aaataaaata atttataccg
      781 aaacatctca ggtaaagaaa aaaatcagtt gctgtatttc ggattgtcgg aagatgattt
      841 taaaaataaa agcatccgtg ctttttgttt actttaaata tgctgctgcc tgattgctgt
      901 agaatgtctt tgactcaact atttgtaaaa acaattcctt agcagcagac ttattatcgg
      961 tgttccataa acatacagct ttatgaaacg tggcttcttc caaaaacgga ttggtttgca
     1021 tttctataat tttatcgaac cataatattg catttgaatg gtcgttcaaa ttttcgagcg
     1081 caatgccttt gtaaaaaata caatttgcat ccattggttt tactacgagt atatcatcga
     1141 acatggcaat ggcattggtg ttttcatttt cgtgaatggc ttgcatagcg cgagccagca
     1201 aaattctata gtcaacagta ttggtattgg ccagtatatc atttttggca ttgccgttat
     1261 cacggttttt gaattgtgcc tgcaagcctg aatttatttt tgccgattct tcatcgctgt
     1321 aaaggtttgt ataatcaata gtttttagcc cgctcatgta aatgatgtta ttgtacgtag
     1381 catccatatt ttttaaatcg ggaattgtcg catccgaaat ttcaattctt gcagggttca
     1441 ctgcacttaa tggcgatatg ctttcgatgt gtttgtgtac atcttttttt gctgcattgt
     1501 actcgaggta agtgattatg gtttcgtttt tattttcgat ggttgtaaaa gttggcgcca
     1561 caaatttttc gccttgaggt tttataattt gctcacttac attggttgga tttgcagatt
     1621 ttattttttg cctttgatta ataaaatacc agaagtaagc acagcaaaaa ataagcaatg
     1681 ctgtaataat caccactacc ggtagattgt gtgttggctt aagtggtttg tgttttagcg
     1741 attttgatgt gcgggctgtc cattgggttt ttatatcttt tatatgaatg ggaaatggca
     1801 taagtgaaac cccttccata gcctcgctgc acagtgcgca caatgcaata tgattgcgac
     1861 acctttgcgc ttgttctcct ttcatattgc ccgcttgcaa tgccagcatt tcatcggcac
     1921 tcaggtggtt caatatttca ctattgctat tatcggcttt catttttttt gttgagtaaa
     1981 taatttttca aattcctttt tccgttttgt ataaaacttt ttacctgatt taagtcgtat
     2041 ccggtttgtg tacttatttc catataagat tttttttcga gataaaataa ttgaatacag
     2101 attcgttgtt gttcatttat cgattctatg gcttcgggca gagttggcag gtgagcata
//
LOCUS       DAORQA010000299         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11278_length_2159_cov_8.214829, whole genome shotgun sequence.
ACCESSION   DAORQA010000299 DAORQA010000000
VERSION     DAORQA010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11278_length_2159_cov_8.214829"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PK736_09265"
     CDS             complement(<1..329)
                     /locus_tag="PK736_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33615.1"
                     /translation="MIKKILLLILLFVSLQAIQKATAQPCNLPTMNMHTVYIFTTKTK
                     HTMEEVMGAKLNPMLFNFVFVKSNEQLATIKQQAIDKKLSSTVFVFFGNGKAIRYCNV
                     EEFVKAV"
     gene            complement(530..2077)
                     /locus_tag="PK736_09270"
     CDS             complement(530..2077)
                     /locus_tag="PK736_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33616.1"
                     /translation="MKTIIILMCICSFEFTNAQNKNINWTFGDSAGINFSNFNQPTTF
                     ITSAKSRGSCASISDSAGSLLFYYAYDPNALLGGSLYNGNVYDKNNQIMQNGDYIVTL
                     AWYYEGIIIPIPNETDKYYLFNIGVATKFGLYYSIIDMSLNNGLGAVTQKNIPINNLD
                     ANDGLTAVQHGNGRDWWLLFRKYDGVWDNTFYKYLIDINGVTCVDTQNIGFVYSTNST
                     RYVFNKLGNKLAGIYYNGHSETFDFDRCTGQLSNNIVLKTNGTTLPPDENLSCEFSDN
                     GRFLYIATSGYYNAAYLIQVDLLNPQLYAAADTIDSINYVLEPGGQLKRGPDDKIYWA
                     CGYTDGFNFPYPYPDTLYNTYNTYLSVINSPDSLGAACNFTPYSFYLGGARTYWGLPN
                     IPDYDLGPLKGSPCDTLQWVGTPETPKPPLGGLNAGLLQCTYISAWEKLFVNASGLKG
                     KNVTVSIYDATGKQKYVAGKSPKGDLGVHSGYATVDVDCAGWSDGLYVVHLQTEKEVL
                     STKFVKE"
BASE COUNT          647 a          436 c          386 g          690 t
ORIGIN      
        1 tctacagcct ttacaaactc ctccacattg cagtaacgta tagccttacc gttaccgaaa
       61 aaaacaaaaa ctgtgctcga aagttttttg tcgatagctt gttgttttat tgtagcaagt
      121 tgctcgttac tttttacaaa cacaaaatta aaaagcatgg ggtttaattt tgctcccatc
      181 acttcttcca tagtgtgttt cgtttttgtg gtaaaaatat acactgtgtg catattcata
      241 gtgggtaagt tgcatggttg tgctgttgct ttttgaatgg cttgtagcga tacaaaaagg
      301 agaattagaa ggaggatttt ttttatcact gcgcgggaac ttattgtgct caacatagtt
      361 ttattttaat atattaattt ttagtttcat ttggcacttc gcacccctgt ccaccgatac
      421 tgcggacggt gcaggcctaa agggagtctg ccacaagtgc taacaaaatt ttcttttaaa
      481 cgatacattg gcacccttca gggttacggg tgagcgttgg tcgtatttct tattctttaa
      541 caaacttagt actcaacact tccttttccg tttgtaaatg tacaacatac aacccatcac
      601 tccaacctgc acaatcaaca tctactgtgg cgtaaccaga atgcacccct aaatcccctt
      661 taggggactt gcccgctaca tacttttgtt tgccggtagc atcgtatata ctcaccgtta
      721 cattcttccc tttcaatcca cttgcattaa caaacaactt ttcccaagcc gaaatatacg
      781 tgcattgcaa aagccccgca tttaagcccc ctaaaggggg tttgggggtt tcgggcgtgc
      841 caacccactg caaagtatca caaggactcc ccttcaacgg ccccaaatca taatccggaa
      901 tatttggcaa accccaatac gtacgtgcgc ctccaagata aaaactataa ggtgtaaagt
      961 tgcatgcagc gccaagacta tcgggtgagt ttattacact taggtaggtg ttataggtat
     1021 tatacaaagt atcagggtaa gggtaaggga aattaaaacc atctgtataa ccgcaagccc
     1081 aataaatttt atcatcgggg ccgcgtttta attgaccacc cggttctaaa acatagttga
     1141 tactatcaat ggtgtcggcc gctgcatata attgtggatt aagcaaatct acttgtatta
     1201 aatatgcggc attgtagtat ccgcttgtgg cgatgtataa gaacctgcca ttatctgaga
     1261 attcgcaact caaattttca tccggtggga gtgtagtgcc attagttttt agcactatgt
     1321 tattggagag ctgacctgta catctatcga aatcaaatgt ttcggagtga ccattgtaat
     1381 aaatacctgc caatttgtta ccaagtttat taaatacata tcttgttgaa ttggtgctgt
     1441 aaacaaaacc aatattttgt gtatcaacac aagtaacgcc atttatgtca attaaatatt
     1501 tataaaaagt attatcccat acaccatcgt attttctaaa cagcagccac caatctctgc
     1561 catttccatg ctgcacagcg gtgaggccat cattggcatc caaattgttt attggaatat
     1621 ttttttgtgt tactgcacct aagccattgt tgaggctcat gtctatgatt gagtagtaga
     1681 ggccaaattt cgttgctaca cctatattga ataaatagta tttgtccgtt tcatttggga
     1741 taggaataat aattccttca taataccaag caagcgtaac aatgtaatcg ccattttgca
     1801 ttatttgatt atttttatca taaacattgc cattatataa actaccgcca agcaatgcat
     1861 tcgggtcata agcgtaataa aacaataaac tgcccgctga gtcactaatt gaagcacaac
     1921 tacctcgcga ctttgcactt gtaataaatg tagttggttg attaaaatta ctaaaattga
     1981 tgccagccga atctccaaac gtccaattaa tatttttgtt ttgggcgtta gtaaattcaa
     2041 aggaacaaat gcacattagg attattattg ttttcatatt taagcaaata aaaaagcgca
     2101 cattttactg tgcgcttttg tggataatca taatttaata accgtagaat taaatttaa
//
LOCUS       DAORQA010000300         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11284_length_2159_cov_3.798954, whole genome shotgun sequence.
ACCESSION   DAORQA010000300 DAORQA010000000
VERSION     DAORQA010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11284_length_2159_cov_3.798954"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>531)
                     /locus_tag="PK736_09275"
     CDS             complement(<1..>531)
                     /locus_tag="PK736_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HRC33617.1"
                     /translation="STNWITETMACYQHDTNAVLGYGAYEKKPGFLNALIRFDTAANA
                     SLFLSQAIRGKAYMGVGRNLSYHKKIFFDNKGFANHQHLLSGDDDLFINDISSQSKIE
                     VCVNPDAFTYSVPKQTFGAWFTQKVRHVSTSKSYKTRDQRFLATYHLTHILFWLCAIV
                     LLAFQYQWQLVLAIIG"
     assembly_gap    532..592
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<593..1149)
                     /locus_tag="PK736_09280"
     CDS             complement(<593..1149)
                     /locus_tag="PK736_09280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC33618.1"
                     /translation="MMINVEDENFCIACKVIQTHFANKIIHNYHFINYGKLAQQYHIV
                     NFTFAPSIHNMHSQLESLPYFSQALLYGPVAILALLALIQLFYFLFVQLRLAMYKSTF
                     TASTKPPVSVIVCARNEFDNLEKHLPSLLNQEYPQYQVVVVNDCSWDNSAKYLEQMAA
                     THSHLKIVTIKEQEKYRHGKKFALT"
     gene            1189..>2159
                     /gene="tgt"
                     /locus_tag="PK736_09285"
     CDS             1189..>2159
                     /gene="tgt"
                     /locus_tag="PK736_09285"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRC33619.1"
                     /translation="MQFEVKVHDRDTKARAGLITTDHGTIETPIFMPVGTAGTVKGMS
                     QRNLTDDVQAQIILGNTYHLSLRPGMDVMQQAGGLHKFMNWQRPILTDSGGYQVYSLA
                     ANRKIKEEGVKFRSHIDGSPQFFSPESAIEIQRQIGADIIMAFDECTPYPCDIRTARK
                     SMELTHRWLLRCVQYFDSNDPLYGHSQTLFPIIQGSTYPELRKISAEFVLQTNREGYA
                     IGGLSVGEPVEDMYAMTDMVCDILPKDKPRYLMGVGTPANILECIALGIDMFDCVMPT
                     RNARNGMLFTTQGIINIRNEKWKSDFSPIDPAGLCYADTHYTKAYLR"
BASE COUNT          607 a          422 c          465 g          604 t
ORIGIN      
        1 aacctataat ggccagtacc aattgccatt gatattgaaa tgccagcaac acaatagcac
       61 aaagccaaaa taaaatatgt gtaagatgat atgttgccaa aaaccgctgg tcgcgcgtct
      121 tgtacgattt cgatgtgctc acgtgcctca ccttttgtgt gaaccaggca ccaaaagttt
      181 gcttgggaac cgagtaagta aatgcatcgg gatttacaca cacttcaatt ttcgattgcg
      241 atgaaatgtc atttataaat aaatcatcat cgccagaaag taaatgttga tgattggcaa
      301 atcctttgtt atcgaaaaat atttttttgt gatacgatag attgcggccc acacccatgt
      361 atgctttacc acgaatggct tgcgataaaa ataaacttgc attggctgca gtatcaaagc
      421 ggataagtgc attgaggaat cccggttttt tttcgtacgc accataaccc aacacagcat
      481 tggtatcgtg ttggtagcag gccatggttt cggtaatcca attggtagaa gnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgtgtaag
      601 tgcgaatttt ttaccatgac gatatttttc ctgctccttg atagttacaa tttttaaatg
      661 cgaatgtgtg gccgccattt gctccagata ttttgcactg ttatcccatg agcaatcgtt
      721 tacaactaca acctgatatt gaggatattc ctgatttagc aaagagggca aatgtttttc
      781 gagattatca aattcgttgc gggcacaaac gataaccgat accggtggtt tagtacttgc
      841 cgtaaaagtg cttttataca ttgccaaccg caattgcaca aacagaaaat aaaataattg
      901 tataagtgct aataatgcaa gtatggcaac gggaccgtaa agcaatgctt gcgaaaagta
      961 aggtaatgat tctaactggc tatgcatatt gtgtattgag ggcgcaaatg taaaatttac
     1021 aatgtgatat tgctgtgcaa gttttccata gtttataaag tggtaattgt ggataatttt
     1081 atttgcaaaa tgtgtttgta tcactttaca tgcgatacaa aaattttcat cttccacgtt
     1141 tatcatcatg cataatccgt ttgtgcaata ttgctacttt tgcgcgctat gcaatttgaa
     1201 gttaaagttc acgatagaga taccaaagcc cgcgcagggc ttatcaccac cgatcatggc
     1261 actatagaaa cgcccatttt tatgcccgtt ggtacggcag gaaccgttaa aggtatgagt
     1321 cagcgcaatc ttacagacga tgtgcaggcg caaataattc ttggcaatac ttatcatcta
     1381 agtttgcggc ccggtatgga tgtaatgcag caggccggag ggttgcacaa atttatgaac
     1441 tggcagcggc ctattcttac cgacagcgga ggctatcagg tgtattcgct ggcagcaaac
     1501 agaaaaataa aagaagaggg agtaaagttc cgttcgcaca tcgatgggtc gccacagttt
     1561 ttttctccgg agagcgctat cgaaattcaa cggcaaatag gtgccgacat tattatggcc
     1621 tttgatgaat gtacacctta cccttgcgat atacgtactg cccgcaagtc gatggaactt
     1681 acacacaggt ggttgctgcg ttgtgtgcaa tatttcgata gcaacgatcc tttgtatgga
     1741 cattcgcaaa cattgtttcc cattattcaa ggcagcactt atcccgagtt gcgcaaaata
     1801 tcggccgagt ttgtactgca aaccaatcgc gaagggtatg ccattggcgg ccttagcgta
     1861 ggcgaacctg ttgaagatat gtatgccatg accgatatgg tgtgcgatat tttgccaaag
     1921 gataaacccc gctacctgat gggcgtgggc actccggcaa atattcttga atgcattgct
     1981 ttgggcattg atatgtttga ttgtgtaatg cctacacgca atgcgcgcaa cggtatgttg
     2041 tttaccacac aaggcattat caatattcgc aacgaaaaat ggaagagcga tttttcgccc
     2101 atcgatccgg cagggttgtg ctatgccgat acacactata caaaggcgta tttgcggca
//
LOCUS       DAORQA010000301         2158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11288_length_2158_cov_4.398003, whole genome shotgun sequence.
ACCESSION   DAORQA010000301 DAORQA010000000
VERSION     DAORQA010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11288_length_2158_cov_4.398003"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(767..840)
                     /locus_tag="PK736_09290"
     tRNA            complement(767..840)
                     /locus_tag="PK736_09290"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(804..806),aa:Asn,seq:gtt)
     gene            1069..>2158
                     /locus_tag="PK736_09295"
     CDS             1069..>2158
                     /locus_tag="PK736_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRC33620.1"
                     /translation="MQYIYFDNNSTTPLHAKALEAMMPYFTEKYGNAASATHRFGWQA
                     AAAVDIAQSQVAESLGVEPAEITFTSGATESINMALRGLFESYKRKGNHIIVCKTEHK
                     AVLDTCAFLQTCGASISYIDVHRDGIIDLAQLESELSDHTIMVVVMLANNETGVIQPI
                     EKIAELAHSKGAIMVCDTTQAIGKMRCSIPDLNVDVAFISAHKFYGPKGTGAIYLRRK
                     NPRVTIPPLLYGGGDEKHLRPGTMNVPAIVGFGKACTLAIDNLWEYGSHTSRLRTLFE
                     QIFTEDDVAFINGSTRERLPNTTNLCFQNVTAQQIIKHIDNVAVSTGSACTSALAQPS
                     HVLLAMGLSQTQAQNSIRFSFGIQNTLEE"
BASE COUNT          675 a          402 c          404 g          677 t
ORIGIN      
        1 ttacaaagac ataatataaa tgttgcgtaa aactaaatat cagtttcacc accaaagcgt
       61 taatgatgtt tgttttctat aaagttatgc aacacttttc tattttattc tactcaattt
      121 tatgtgctgg caattccgta ttcattaagc atatccgaaa atggaaacga tttcaaaacc
      181 ttctacttcc tcattaaact atggaaaaat catttccgtt agcggtacct gcttcattgc
      241 ctatgtttga atttttcaca ctattttatt aaaagtaaaa taaaaaaaat gaaaattcaa
      301 acaataggtt attcgttaat taaatcagtg gtgggaataa tttatttatt gatggtgatt
      361 ttatttgttg cattagtgta agtattttag aagatgtaag agagcaattt catttgttta
      421 ttttttatat taaaggtgtt tttagtggtt aaggtataat tttaaaatag cagtatcaaa
      481 ttgattaaag tgatactgat ttaaaatatt agttatttta aaggatgtaa gttatttata
      541 ctgtacatcc ttttctttca acatgaaaag cacttgataa caagatggaa atcctttttc
      601 aattaatcct gaaaagattg tggtgtgctt cgcacactta tattaattcc tttggttgtt
      661 ttcaggtttc ttttttcggc tcttggtaca ttgaaataaa gtcacatctc cgttcgcaaa
      721 agcaaaaggg gtcacaacct ttcggtgtaa cccctacata atagtgctcc ccctcctgga
      781 ctcgaaccag ggaccccatg attaacagtc atgtgctcta accagctgag ctaaggagga
      841 atttagtttt ttcggataaa tcgtgtctta aatcacggtt tacccgacta ttcctctttc
      901 ttatctcaaa cccatttcaa caacagttga aaccagaaca cgtgtaatca cgtttttatt
      961 tcaaagggag cgcaaaagta aaacttttat ataaatacta aaaaaaaata taaaataaat
     1021 attttatccc tcgtgtgcct cgatttttga ttgttacttt gaggcaatat gcaatacatt
     1081 tattttgata ataattctac cacaccgctt catgcaaagg cgctcgaagc catgatgcct
     1141 tattttactg agaagtacgg caatgcggcc agcgccacac ataggtttgg atggcaggca
     1201 gcagcagcgg ttgatatagc ccaaagccag gttgctgaaa gccttggcgt tgagccagcc
     1261 gaaattacct tcacatcagg cgctaccgag tctataaata tggcgcttcg cggcttgttt
     1321 gaatcttaca aacgaaaagg aaatcacatt atagtatgca aaacagagca taaagcagtg
     1381 ttggatactt gcgctttttt gcaaacatgt ggagcttcca tttcatatat tgatgtgcat
     1441 cgcgatggca ttattgacct tgcccaactt gaaagtgagc tatcggacca tacaataatg
     1501 gtggtggtaa tgcttgccaa taacgaaaca ggtgttattc aacccatcga gaaaattgcc
     1561 gaattggctc attcgaaagg ggcaataatg gtttgcgata ctacacaagc tatcggaaaa
     1621 atgagatgtt ccatccccga tttaaatgtt gatgtggctt tcatatcggc acataaattt
     1681 tatggcccaa aaggaacagg cgcaatatat ctgcgcagga aaaatccccg ggtaacaatt
     1741 ccacctttat tgtatggtgg cggtgatgaa aaacacctgc gcccgggaac aatgaatgtg
     1801 ccagcaatag tcggatttgg aaaagcatgc acattggcca tcgacaactt gtgggagtat
     1861 gggtcacaca cttcgcgctt gcgtacatta ttcgagcaaa tattcaccga agatgatgtt
     1921 gcatttatta atggttccac aagagagcga ttacctaata ccacaaactt gtgttttcaa
     1981 aatgtaactg cgcagcaaat aattaagcac atcgacaacg ttgcagtttc tacagggtcg
     2041 gcatgtactt cggctttggc acaaccttcg catgtgttgt tagctatggg gctatcccaa
     2101 actcaggcac aaaactctat tcgctttagt tttggaattc aaaatacatt agaagaaa
//
LOCUS       DAORQA010000302         2154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11302_length_2154_cov_12.865650, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000302 DAORQA010000000
VERSION     DAORQA010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11302_length_2154_cov_12.865650"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>865
                     /locus_tag="PK736_09300"
     CDS             <1..>865
                     /locus_tag="PK736_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRC33621.1"
                     /translation="QMWQEFFGRGIVKTSGDFGMQGELPTHPELLDWLAVDFMQNGWN
                     IKRLVKQVALSATYRQSVKTNNEKQKRDPDNIYLSRGPRNRLPAEFIRDLVLSSSGLL
                     IKTIGGPSVKPYQPKGLWEGATSGRGELATYKQDKGDDLYRRGMYTFIKLTLPPPGMA
                     LFDASNRDQCEVKRSKTNTPLQALMMMNDPTVLEASRVLAQKLMDEKSSTNEKIKKAF
                     RLIICRKPNAKEDEILTAYFNEQLQFFKTNKLKADSTIKVGEFPFTKIADQTSFASLM
                     KTINMIYNMEEA"
     assembly_gap    866..875
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            892..>2154
                     /locus_tag="PK736_09305"
     CDS             892..>2154
                     /locus_tag="PK736_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRC33622.1"
                     /translation="MKKETLEYGLNLNRRKFLSRLSLGIGSVALGSLLIPDLFGGEHT
                     EETILNHLPQFAPKAKRIIYLFQNGAPSQLDLFDYKPKLQEMFGQELPASIRNGQRLT
                     GMTADQTKFPLAGSLFDFKQHGKAGAWFSNLLPYTAAIADDLCIVKTMFTEAINHDPA
                     LTFFQTGAQVGNRPSMGSWLSYGLGSENKNLPAFCVLLSKGKGNGQGVYSKLWTNGFL
                     DSIHQGVQFSSGDNPVLYLNNPSSINTTDRRKMIDKLNDLNEASFTEFGDPEITTKIQ
                     QYEMAFRMQTAVPEITDMSKEPESIVKLYGPECLIPGTYAANCLLARKLSENGVRFVQ
                     LYHQGWDGHNNLPSEIRTQCMDVDQASAALITDLKQRGLLDETLVIWGGEFGRTNYCQ
                     GTLTKDNYGRDHHPRCFTIWMAGGGVKPG"
BASE COUNT          721 a          398 c          454 g          571 t
ORIGIN      
        1 tcaaatgtgg caggaatttt ttggacgtgg cattgtaaaa acttcgggcg actttggcat
       61 gcaaggtgaa ctacccacac atcccgaatt gttggattgg cttgctgttg attttatgca
      121 aaacggatgg aacataaaac gactggtaaa acaagttgca ctatcggcaa catacaggca
      181 atcggtaaaa acaaataacg aaaaacaaaa aagagatcct gataatattt acttatcacg
      241 cggaccaaga aatcgtttgc cggcagaatt tataagagat ttagttttaa gcagtagtgg
      301 tttacttata aaaacaattg gtggcccaag tgtaaaacca tatcagccca aaggcttgtg
      361 ggaaggcgct acatcgggac gcggtgaact ggcaacttat aaacaagata agggcgatga
      421 tttatatcgc agaggaatgt atacgtttat caaactaacc ttgccacctc cgggtatggc
      481 attgttcgat gccagcaacc gcgatcagtg tgaagtaaag cgctcaaaaa caaacacacc
      541 attacaagca ttgatgatga tgaatgaccc tacagtactg gaagcatcgc gtgtattggc
      601 acaaaaacta atggacgaaa aatcatcgac caatgaaaaa ataaaaaaag cttttcgatt
      661 aattatttgc cgcaagccaa acgcaaaaga agacgaaatt cttacggcat actttaacga
      721 gcaattacag ttttttaaga caaacaaatt gaaagccgac tctacaataa aagttggtga
      781 gtttccattt acaaaaattg ctgaccaaac atcttttgca tctttgatga aaaccataaa
      841 catgatttat aatatggaag aagcannnnn nnnnnttaca aaaacatagt gatgaaaaaa
      901 gaaacgttag aatatggtct aaatttaaac cgcagaaaat ttttgtcgcg gttaagtttg
      961 ggaattggta gtgttgcatt gggttcatta ctaatacccg atttatttgg tggcgaacat
     1021 accgaagaaa caatattaaa tcacttgcct caatttgctc caaaggcaaa acgcataatt
     1081 tatttatttc aaaatggtgc accatcgcaa ttagatttat ttgattacaa acccaagctg
     1141 caagaaatgt tcggacaaga acttccggca tctatacgca atgggcaacg cttaacaggc
     1201 atgactgccg accaaacaaa atttcctttg gcaggaagcc ttttcgattt taagcaacat
     1261 ggaaaagccg gtgcctggtt tagtaattta ctgccttaca cggccgctat tgctgatgat
     1321 ttatgtattg taaaaacaat gtttaccgag gccataaatc acgaccctgc attaactttt
     1381 tttcaaaccg gtgcgcaagt tggtaatcgt cccagtatgg gttcgtggct gagttatgga
     1441 cttggcagcg aaaataaaaa ccttccggca ttttgtgttt tactttcaaa aggaaaagga
     1501 aacgggcaag gcgtatattc taaattatgg acaaatggat ttttagattc cattcatcag
     1561 ggtgtgcaat ttagtagtgg cgataatccg gtgttgtatc tcaacaatcc ttcgagcata
     1621 aatacaacag accgcagaaa gatgattgat aaattaaatg atttaaacga ggcttcattt
     1681 acagaatttg gtgacccgga aatcactaca aaaattcaac aatatgaaat ggcttttcga
     1741 atgcaaactg cagttcctga aattactgat atgagcaagg aacccgaaag cattgtaaag
     1801 ttatatggcc ccgaatgttt gattcccggc acgtatgcag ccaactgttt gctggcgcga
     1861 aagctatcag aaaacggtgt gcgctttgta caattatatc accaagggtg ggatggacat
     1921 aataatttac cgtccgaaat tcgtacacaa tgtatggatg ttgatcaggc aagcgcagca
     1981 ctaataactg atttaaaaca acgtgggttg cttgacgaaa cacttgtgat ttggggaggc
     2041 gaatttggcc gtacaaatta ttgtcagggc actttaacaa aagataatta tggccgggac
     2101 catcacccgc gttgctttac tatctggatg gcaggcggtg gcgtgaaacc ggga
//
LOCUS       DAORQA010000303         2152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11314_length_2152_cov_5.993324, whole genome shotgun sequence.
ACCESSION   DAORQA010000303 DAORQA010000000
VERSION     DAORQA010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11314_length_2152_cov_5.993324"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="PK736_09310"
     CDS             <1..1264
                     /locus_tag="PK736_09310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRC33623.1"
                     /translation="IKVAHIMVKVPTGVSDTDSASITAKSKIMEIAAKLKAGESFASL
                     AEQFSDDKGSAKNGGELPFFGTGRMVPEFEKAAFALKADGDVTEPIKSSYGWHIIKRV
                     EKKAVQTFEEKKADLKQQITRDSRSEVSKMSMINKIKKEYSFNENTAAREEVLLSLDS
                     NVVKGDYAPDSAKSWSKNICTIGTKTLTQQDFAEYIVTHQIRRTSGEPYAIGYNLYEG
                     FVNDACIEYEEGRLEQKHDEFKNLMQEYRDGILLFDLTDKMVWSKAVKDTVGLKEFYE
                     KNKNNYMWGERTKATVYTCENAVVAEAVRKLIKKKMADADILTEINKDSQLNLTIKDG
                     KFAKGENENVDAAKVSKGLSPNVEKNNQVVFVNIIENMPAMPKAMDEAKGLITADYQN
                     YLEKNWIGELRKKYPVTVNEQLLQTIIK"
     gene            1362..>2152
                     /locus_tag="PK736_09315"
     CDS             1362..>2152
                     /locus_tag="PK736_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33624.1"
                     /translation="MKLIKILFLTILVPVICSINGCGMLDDKSSKKNEGRQLARAFDD
                     YLYEIDIVDLVPPNTKADDSLMIVKSYIDNWIKEKVLLHKAQESIGDYEKQVESQVSQ
                     YRNSLITYAYQKAIIKKQLDTLVNEAEITKYYEDNKNNFLLKENITKCIYAKFYSNTQ
                     DLKKLKQWMVSSVEADREKVDNFCLQYAFDYFLNDDTWLSFADLMLRTGIKTYNQEQF
                     LQSTKVIEVPDSGSVTIVRFYDFKIKESLSPLSFENNNIRNLIIN"
BASE COUNT          789 a          347 c          444 g          572 t
ORIGIN      
        1 aataaaggtt gcacacataa tggtgaaagt gcctacaggt gttagcgata ctgacagcgc
       61 aagcataact gcaaaatcaa aaataatgga aattgccgca aaattgaaag caggagaaag
      121 ttttgcaagt ttggcagagc aattttctga cgataaaggc tctgcaaaaa atggtggtga
      181 attacctttc tttggaacag gccgtatggt tcctgaattt gaaaaagctg cattcgcttt
      241 aaaggccgat ggtgatgtta ccgaacctat aaaatcttca tacggttggc atataattaa
      301 aagggttgaa aagaaagccg tacaaacttt cgaagaaaag aaggccgatt tgaaacagca
      361 gattacgcgc gatagccgca gcgaggtgag taaaatgagc atgatcaata aaataaaaaa
      421 ggaatatagc tttaatgaaa atactgccgc gcgcgaagaa gtattgcttt cgctcgattc
      481 aaatgtagtg aagggcgatt atgctcctga tagtgctaaa tcatggtcga aaaatatatg
      541 tacgatagga actaaaaccc tcacacagca agattttgct gaatatattg taacacatca
      601 aatacgcaga accagtggcg aaccttatgc tataggatac aacctgtacg aaggatttgt
      661 aaacgatgcc tgcattgaat acgaagaagg ccgccttgaa caaaagcacg atgaatttaa
      721 aaacctgatg caggaatatc gcgatggtat tttacttttc gatcttactg ataaaatggt
      781 gtggagcaaa gccgtaaaag acactgttgg gcttaaagag ttttacgaaa aaaataagaa
      841 taactatatg tggggcgagc gcaccaaagc aactgtttac acttgcgaaa atgccgtggt
      901 tgccgaggcg gtgcgtaagt tgatcaagaa aaaaatggct gatgccgata tccttaccga
      961 aatcaataaa gattcgcaac tcaaccttac aatcaaagat ggtaaatttg caaaaggcga
     1021 aaatgaaaac gtagatgctg caaaagtttc taaaggattg tcgccaaatg tggaaaaaaa
     1081 taatcaagtg gtgtttgtaa acattattga aaatatgcct gcaatgccaa aagctatgga
     1141 cgaagcaaag ggattaataa ctgccgatta tcaaaattat ttagagaaaa actggattgg
     1201 tgaattgcgc aaaaagtatc ctgttactgt taatgagcag ttgcttcaaa ccattatcaa
     1261 gtagtcgata aaatctcatg tttttattgg ataaagcgaa gcatatttgt ttcgctttat
     1321 ttgtttaaaa cctttcgatg taattttgaa aagattgaaa attgaaacta attaaaatat
     1381 tatttcttac aatattggtt cctgttatct gcagcataaa cggatgtggt atgcttgacg
     1441 ataaaagcag caaaaaaaat gaaggccgcc aactggcgcg tgcttttgat gattatctgt
     1501 acgaaattga tattgttgat ttagttccgc ccaatacaaa agctgacgac agcctgatga
     1561 ttgtaaaatc atacattgat aactggataa aagaaaaagt gctgctgcac aaagcgcagg
     1621 agagtattgg cgactacgaa aaacaggttg aaagtcaggt aagccaatac cgcaattcgc
     1681 ttattactta tgcttatcaa aaagccatta tcaaaaagca actcgataca ctggtgaatg
     1741 aggcagaaat aaccaagtat tatgaagata ataaaaacaa tttcctgctt aaagaaaata
     1801 ttacaaagtg tatatacgca aagttctata gcaatacgca agatttaaaa aagctgaagc
     1861 aatggatggt atcatctgta gaagcagaca gagaaaaagt agacaatttt tgccttcaat
     1921 atgcgttcga ttattttttg aatgatgata catggctttc gtttgccgac ctcatgctac
     1981 gtaccggaat taagacatac aatcaggaac agtttctgca aagcaccaaa gtaattgaag
     2041 tgcccgactc gggaagtgtt actattgtac gtttttacga ttttaaaatt aaggaaagtc
     2101 tttcaccatt aagttttgaa aataacaata ttcgaaacct gataataaat aa
//
LOCUS       DAORQA010000304         2143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11370_length_2143_cov_9.575192, whole genome shotgun sequence.
ACCESSION   DAORQA010000304 DAORQA010000000
VERSION     DAORQA010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11370_length_2143_cov_9.575192"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2143)
                     /locus_tag="PK736_09320"
     CDS             complement(<1..>2143)
                     /locus_tag="PK736_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRC33625.1"
                     /translation="NKIYYMDFWVMVSKDGGRTFNKIGEKYKHVDNHALWIDPNDTQH
                     LLVGCDGGVYETYDNGANWNFKSNLPVTQFYKVTVDNATPFYNVYGGTQDNNSLGGPS
                     RTKSANGISNADWFVTQSGDGFESQVDPVDPNIVYAQAQYGALGRYDKKSGEVVDISV
                     VQNDNEPALRWNWDSPLLISNFSNTRLYFGANKLFRSDDRGNTWKAISGDLSRQIDRN
                     KLPLMGKVWSMDAVAKNSSTDFYGQLVTVAESYFDENNIVAGTDDGLIQVTTDGGKNW
                     KQIDNIPGVPARTYVNQIIASPHDKNIFYATFNHHRYGDFKPYIFKSIDGGISWSPIQ
                     NNLPARGTVYSIAEDHVDRNLLFAGTEFGVYFSNTGGQQWIQLKGGMPTIAVRDIAIQ
                     KRENDLVLATFGRSFYVLDDYSSLRNATAETFKGDAHIFPIKEALSYIEETPVGYPLK
                     GFQGESYYATPNPAVGAVFCYYLKDDILTIKEARQKAEKEKIKNGEPPFYPSADSIRM
                     EDAQPAPYLLFTITDESGGVVRKIKAPAKKGMNRIKWDLRYAAPSPVSFQSPDATNPY
                     DQAPIGYMTMPGNYKVSLSKVVDDKITEIVAPQSFVVKALNAATLVASDKKALDAFCK
                     KVNDLFRATSAADAYRSELVSKIKYMKQAVIDAPQPMAGVTQQIQAVEKRLREVDIKL
                     NGDASIARREFETPPGINGRVGNVAFNILNAT"
BASE COUNT          562 a          479 c          420 g          682 t
ORIGIN      
        1 ttgtagcatt caaaatattg aaggccacat tgccaactcg tccatttatt cccggtggtg
       61 tttcaaattc tcgtcttgct atacttgcat cgccattgag ttttatatcc acttcgcgaa
      121 gtcgcttttc aactgcctgt atttgttgtg taactcctgc catgggttgt ggtgcatcaa
      181 tcaccgcttg cttcatatat tttattttac ttacaagttc gctgcggtaa gcatcggcag
      241 ccgatgtggc acgaaacaga tcgttcactt ttttgcaaaa tgcatcaagt gcttttttat
      301 ccgatgcaac aagcgtagcg gcattaaggg ctttcaccac aaacgattgt ggcgctacaa
      361 tttctgttat tttatcatcg acaactttgc ttagcgatac tttataattg cctggcatgg
      421 tcatgtatcc tattggtgcc tgatcatatg ggttggtggc atcgggcgat tgaaaactta
      481 cgggcgatgg cgctgcatag cgtaaatccc atttgattct gttcatgccc ttttttgccg
      541 gagcttttat tttgcgaacc actcccccac tttcatcggt gatggtaaac aaaagatatg
      601 gtgcaggttg tgcatcttcc attctaatcg aatcggcact gggataaaaa ggcggctcgc
      661 cattttttat tttttctttt tctgcttttt gccgtgcttc ttttatagtg agtatatcat
      721 ctttcaaata ataacagaaa acagccccaa cagcgggatt gggagtagca taataacttt
      781 cgccctgaaa ccctttcaac ggatatccca caggtgtttc ttcaatataa gacagggcct
      841 cctttattgg aaaaatatgg gcatcgcctt taaatgtttc ggcagtggca ttgcgcaacg
      901 atgaataatc gtcaagcaca taaaaagatc gtccaaatgt tgcaagcact aaatcgtttt
      961 cgcgtttttg tatcgcaata tcgcgaacag caattgttgg cattccccct ttaagttgta
     1021 tccattgttg cccaccggtg ttggaaaaat aaacgccaaa ttctgttccg gcaaaaagca
     1081 aattgcgatc aacatgatct tcggcaatgg aatacactgt gccacgtgcc ggaagattat
     1141 tttgtattgg tgaccatgaa attccaccat cgatgctttt aaaaatatat ggtttaaaat
     1201 cgccatagcg gtggtgatta aacgtagcat aaaaaatatt tttgtcgtgt ggcgatgcaa
     1261 taatttgatt gacatacgtg cgtgccggca caccgggaat gttgtcaatc tgtttccagt
     1321 ttttgccacc atctgttgtt acctgtatca atccatcatc ggtacccgcc acaatattat
     1381 tttcgtcaaa gtagctctcg gcaacggtta caagctgtcc gtaaaaatct gtggaggaat
     1441 tttttgccac tgcatccata ctccacactt tgcccattaa aggtaattta ttgcgatcaa
     1501 tctgtcgcga taaatcgcca cttattgcct tccatgtatt accgcggtcg tcactgcgaa
     1561 ataatttgtt ggcaccaaaa tataaacggg tgttgctaaa attcgagatc aataatggcg
     1621 aatcccagtt ccaacgcagt gcaggttcat tgtcgttttg cacaacagaa atatcaacca
     1681 cttcgccact ttttttatcg taacggccga gtgcgccata ttgtgcctgt gcatatacaa
     1741 tgttgggatc gacagggtca acctgacttt cgaagccatc gccactttgt gttacaaacc
     1801 aatcggcatt agaaattcca tttgcacttt tggtgcgcga tgggccacca agcgaattgt
     1861 tatcctgtgt gcctccatat acattgtaaa atggtgttgc attatcaact gtaactttgt
     1921 aaaattgtgt taccggcaag ttcgatttaa aattccagtt agcaccatta tcatatgttt
     1981 cgtaaacacc accatcgcag cccacaagca gatgttgtgt atcattgggg tcaatccata
     2041 atgcatgatt atcaacatgc ttatactttt cgccaatctt attaaaagtg cgtccaccat
     2101 ctttcgacac catcacccaa aaatccatgt agtatatttt gtt
//
LOCUS       DAORQA010000305         2141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11380_length_2141_cov_19.488974, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000305 DAORQA010000000
VERSION     DAORQA010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11380_length_2141_cov_19.488974"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..2020
                     /locus_tag="PK736_09325"
     CDS             143..2020
                     /locus_tag="PK736_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HRC33626.1"
                     /translation="MFLKPYRKSRTLTGDQRLYFKLCESYRTADSVRHQVILDLGMLE
                     ALPDVEDKKKLGIRISELVQESRTGMGKLFPPETDVIEKLANAFFDEIKLKKRLDVSA
                     NNKIQAIDTESIENKDVVEVGAEWLCSQALDQLGIGSFLQQKKWSDELIRLAYTHIIS
                     RAVYPASEFKTAKWIKQNSGICEITNYPIQKITKDKLYTISHRLYEQKDDLEKYLSTK
                     TNELFDLEDKILLYDLTNTYFEGAMRESNIAKFGRSKEKRSDARLIVLALVVNTQGFP
                     KYSHIFEGNMSDSKSLGEIIETLSERTSATTRKPTVVMDAGIATEENLKLIISHGYEY
                     ICVSRKAQKHYEIDQNAPEEVLINNNKKLLTVQSVKQKIGTDHVVKVYSSAKEKKEKS
                     MDESFSKKFELGLQNIKDALSKKSGVKQKEKVHERIGRLKQKYPSIHKHYNIDVAYKG
                     NIVIDLNWAIIQHPNTHGIYFIRTSLNKKDNATLWQIYNSIREIESTFRCLKTDIDLR
                     PIYHKTDAASMAHIHLALLAYWTVNAIRHQLKANNINTQWCDIVRTMNTQKLVTTTMQ
                     NQYKQTIVIRQCSEPIKEVQHIYQALNYKSRPFARKKSVVTPANSLSEVAPNNQSSLS
                     G"
BASE COUNT          796 a          438 c          402 g          505 t
ORIGIN      
        1 attgccaatt tggcgaatgg gtggaggtaa aaaatgcaaa ccgttgctaa tactacaaag
       61 gagttgtcat gttttttaaa aagtaaaatg tagtccccct aacttgtttt tataacaaaa
      121 aatttgcaca tagatttgcc gcgtgttcct caaaccatat agaaaatcac gtacacttac
      181 tggcgaccag cgtttgtact ttaaactgtg cgagagttat cgtactgcag atagtgttcg
      241 ccatcaggtg atattagatt taggaatgtt ggaggcgcta cctgatgtag aagacaagaa
      301 aaaacttggc atacgcatta gcgaattggt gcaggagagt cgaacaggca tgggcaaatt
      361 atttccgccc gaaacggatg ttattgaaaa attagcgaac gcattttttg acgagataaa
      421 attaaaaaag cgattggatg tatcggccaa caataaaata caagccatcg atactgagtc
      481 catagaaaac aaagatgtgg tagaagtagg agcggaatgg ctctgctcgc aagcattgga
      541 ccaattggga ataggtagtt ttttacaaca aaagaaatgg agcgatgagc ttatacggtt
      601 agcatatacg cacatcatca gtcgcgcagt atacccagca tcagaattta aaactgcaaa
      661 atggataaaa caaaattcgg gcatatgtga aatcacaaat tatccaatac aaaaaatcac
      721 aaaagataaa ctatacacca tcagccatcg cttatatgag caaaaggatg acctcgaaaa
      781 atatctaagt acaaagacca atgaactatt tgaccttgaa gataaaatat tgttgtacga
      841 cctcaccaat acctacttcg aaggggctat gcgtgagagt aacattgcca aatttggaag
      901 gagcaaagag aagcgcagcg atgcacgatt gatagtgctc gcattggttg taaacacaca
      961 aggctttcca aaatactcgc acatatttga aggcaacatg agcgatagca aaagcctggg
     1021 cgaaattatt gaaaccctat cagagcgcac atcggcaacc acccgtaagc ctacagtggt
     1081 aatggatgca ggcattgcaa cagaagaaaa cttaaaacta atcatcagcc atggatacga
     1141 atatatatgc gtatcgcgca aagcacaaaa gcattatgag atagaccaaa acgcacccga
     1201 agaagtactc ataaacaata acaaaaaatt actcacagta caaagtgtga agcaaaaaat
     1261 cggcacagac catgttgtaa aagtgtactc gagtgctaaa gaaaaaaaag aaaaaagcat
     1321 ggacgagagc ttctctaaaa aatttgaact cggcttgcaa aatataaaag atgctctatc
     1381 aaaaaaaagt ggcgtaaaac aaaaagaaaa agtacacgaa cgcatcggcc gactcaaaca
     1441 aaagtatcct tccatacaca agcactacaa tattgatgtt gcatacaaag gcaacattgt
     1501 tatcgattta aattgggcta tcatacaaca ccccaacaca catggcattt attttatacg
     1561 cacatcgctc aataaaaaag acaatgccac actatggcaa atctacaaca gcattcgcga
     1621 aatagaatcc accttccgtt gtcttaaaac agatatagac ctgcgcccta tataccacaa
     1681 aactgatgcg gcatcaatgg cgcacattca tcttgcacta cttgcatact ggacagtaaa
     1741 tgccattcgc catcaactta aagcaaacaa tataaataca caatggtgcg acattgtgcg
     1801 cactatgaat acgcaaaagc tggtgaccac caccatgcaa aatcaataca aacaaactat
     1861 cgtaattcgc caatgctccg agccaataaa agaagtacaa catatttacc aagcgctcaa
     1921 ttataaatcc cggcccttcg cgcgcaaaaa atctgtagtc accccagcca actctttaag
     1981 tgaggttgct cccaataatc agtcttcttt atcagggtag ctgcaatgtg ggttaaatca
     2041 cttacttata tgcttataat aaatgacgat ggagtttata tccagtcttt gccttttcct
     2101 aataactcaa tagtcaatgg tcaatagtca atagtcagta g
//
LOCUS       DAORQA010000306         2134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11414_length_2134_cov_13.222222, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000306 DAORQA010000000
VERSION     DAORQA010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11414_length_2134_cov_13.222222"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="PK736_09330"
     CDS             <1..1452
                     /locus_tag="PK736_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33627.1"
                     /translation="NRNNAWIFGNNAKLLFTNNSVTVDSAICNTRGTCSAICDTSGDL
                     LFYTYNLTAVPNFYGTYIRNKMDSAIQNSDSIYSRAWYKQHVIIPLPNNDSMYYLIHA
                     PVTGNNGFYYSIIDMSQDSGRGAVIQKNVQLQGANFYANDGITAIKHGNGRDCWVMVK
                     DCYGIKNNLYFFYLVSPTGIQFDHVQAIGDSIKTNILRIEASDDGSKVALITAMGYIS
                     IFNYDRCTGMLSNEISIEKDALLGSLAPWYWDCELSPNKRYLYVSQAYSDFDTTNYLL
                     RFDLQAPNIAASRDTLYAGEESAVAGLIELAPDGKIYWACAYNPPNTFFYPYPDSTHN
                     YITDNLSVINYPDSSFCDFQPFSFYLGGKRTYYGLPNNPNYGLGRLYGSPCDTLQWTN
                     LTPALSKGEGVMQITYISAWEKLFVNASGLKGKNVTITIYDGRGSAIKNYELKIINSY
                     ATLDVDCSGWSDGLYVVHLQTEQEMLSSKFVKE"
     gene            complement(1537..>2134)
                     /locus_tag="PK736_09335"
     CDS             complement(1537..>2134)
                     /locus_tag="PK736_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HRC33628.1"
                     /translation="PIKHISCYALTIEERTALSHFVKTKKINIPTDEIVITQFKELVD
                     WAMNNNFDHYEISNFCQPGFHSRHNSNYWSGKNYIGIGPSAHSYNGNTRQWNIANNNA
                     YIDSVTNSKSYFEAETLTQENKYNEYVMIHLRTKAGVDILQLQQQFGNEYTSHFEKKI
                     AAYVTQNQVVVMQNNYVLTMEGKLFADAIAGDCFVEVK"
BASE COUNT          645 a          361 c          423 g          705 t
ORIGIN      
        1 aacaggaata atgcatggat ttttggcaac aatgcaaagt tgctttttac taacaatagc
       61 gttacagtag atagtgccat ttgcaataca aggggaactt gttcagctat ttgtgataca
      121 agcggtgatt tgctttttta cacatataac ttaactgctg tgcctaattt ttatggaact
      181 tatatacgca acaaaatgga ttctgcaatt caaaatagtg actcgatcta tagtagagca
      241 tggtataaac agcatgtaat tattccattg cctaataatg atagcatgta ttatttgatt
      301 catgcaccag taactggcaa caatggtttt tactactcca taatagatat gagtcaagac
      361 agcgggcgtg gtgcagtaat acaaaaaaat gtacaattac aaggtgctaa tttttatgct
      421 aatgatggca tcaccgccat taaacatggc aatggaagag attgttgggt gatggtgaag
      481 gattgctatg gcatcaaaaa taatttatac tttttttatt tggtttctcc aactggtatt
      541 cagttcgacc atgttcaggc aattggtgat tcaatcaaaa caaatatact tagaattgaa
      601 gcctcagatg atggaagtaa agttgcttta attacagcta tgggctacat tagtatattt
      661 aattatgata ggtgcacagg aatgttatca aatgaaattt caattgaaaa agatgcttta
      721 cttggaagcc ttgccccgtg gtattgggat tgcgaactat ctcccaacaa acgctactta
      781 tatgtaagcc aagcatacag cgatttcgat actactaatt atttattgcg gtttgatttg
      841 caagctccta atattgctgc aagtagagat acattgtatg ccggagaaga aagtgccgtt
      901 gcaggcttaa tagagcttgc tcccgatggt aaaatatatt gggcttgtgc atacaatcct
      961 cccaatacat ttttttatcc ttaccccgat agcacacata attacataac agataattta
     1021 agtgttatca attatcccga tagtagtttt tgcgattttc agcccttctc tttttacctc
     1081 ggtggcaagc gtacgtatta tggcttgccc aataatccta attatgggtt agggcggctt
     1141 tacggttcac cttgtgatac tttgcagtgg acaaacctca ccccggccct ctccaaagga
     1201 gagggagtaa tgcaaattac atacatttcg gcttgggaga agttgtttgt aaatgcaagt
     1261 gggttgaaag ggaagaatgt aactataacc atatacgatg ggcgcggtag cgcaattaaa
     1321 aattatgaat taaaaattat aaatagctac gcaactttgg atgtagattg cagtggttgg
     1381 agtgatggct tgtatgttgt acatttacaa acggagcaag aaatgttgag ctctaagttt
     1441 gtgaaggagt aaaatatctt tatcaatttt tgtaaaataa tgctttgggt tgaggcccta
     1501 accacaggca gaaactggca agtatttata ctaaagctat ttcacctcta caaagcagtc
     1561 gccagcaatg gcatcggcaa ataattttcc ttccatagta agtacataat tattttgcat
     1621 aaccacaacc tggttttgtg ttacataggc cgctatcttt ttttcaaaat ggctggtata
     1681 ttcgttgccg aattgttgtt gcaattgcaa aatatcaact cccgcttttg ttcgcaggtg
     1741 aatcatcaca tactcgttgt atttattttc ttgagttagt gtctctgctt cgaaataaga
     1801 ttttgaattt gttacactat caatgtaagc attgttattg gcaatattcc actgcctggt
     1861 attgccgtta taagaatgtg ccgaaggacc aatgccaata taatttttcc cactccagta
     1921 attgctattg tgccgcgaat gaaaaccggg ctggcaaaaa ttagaaattt cgtaatggtc
     1981 gaaattgtta ttcattgccc aatcaacaag ttctttaaat tgtgttatta caatttcgtc
     2041 tgttgggatg tttatttttt ttgtttttac aaaatgtgat agtgccgttc gttcttcaat
     2101 ggtaagtgca taacatgaaa tatgctttat gggt
//
LOCUS       DAORQA010000307         2134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11419_length_2134_cov_5.825878, whole genome shotgun sequence.
ACCESSION   DAORQA010000307 DAORQA010000000
VERSION     DAORQA010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11419_length_2134_cov_5.825878"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PK736_09340"
     CDS             <1..578
                     /locus_tag="PK736_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33629.1"
                     /translation="YKMKRLFFIMTLAFAWSCTANSQTKKASTRSSLTVSLDEADGAK
                     KEEYVIDRNDSTIFFKMENSQLMFLKINGDKIPTADYAKYMPLVNTIRTEYKANENER
                     AERVKERIADMHNTIRKSDGTSYSISYSPNNKGMLTDDTQTRYYGEKYEIVVEQDKII
                     KLYYEGKEVAADEIPNHKEACDRVMRNAMKK"
     gene            611..1861
                     /locus_tag="PK736_09345"
     CDS             611..1861
                     /locus_tag="PK736_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRC33630.1"
                     /translation="MDRVIEKKKWPASRIAFIAGILLFAAFVLYTFLFSSGGKKLNVE
                     GERISISEVKQDDFREYIPVNGLVLPVTSIYLDALEGGRVEEKFVEDGAQLKKGDAIL
                     RLSNTDLELSLANEQTAVFNVLTQMQNTKNNAEQNSINRQTNMADIDNALKEAERIYN
                     TNKKLFDDKVIAEQDFKSTENLYNYQVTRKKLAEDIMKQDGNSMKQQVVQMKESYERM
                     QHTLALMQRKVGDLIVRAPVDGQITSLDAEYGQSINKGQRLGQIDVMSGFKVRAEIDE
                     HYISRVFPDLQGEILLGEKTYHLKIKKVYSQVSNGRFQVDLVFIDAVPQNIRRGQTLQ
                     IRLALGDETKALLVNKGGFYQQTGGNWAFKLSADGKKAYKVDMQVGRQNPDYYEILSG
                     LKSGDKIITSSYETFGDMEELEIK"
BASE COUNT          758 a          371 c          454 g          551 t
ORIGIN      
        1 tttataaaat gaaaagatta ttttttatca tgacattagc ttttgcatgg agttgcactg
       61 caaatagcca aacaaaaaaa gccagcacca gatcaagttt aacagttagc cttgatgagg
      121 ctgatggcgc taaaaaagaa gaatatgtta ttgacagaaa tgacagtact atattcttca
      181 agatggaaaa ttcgcaattg atgtttttga aaatcaatgg cgacaaaatt ccaactgctg
      241 attatgccaa gtatatgcct ttggtaaata ctataagaac tgagtataaa gcaaacgaaa
      301 atgaaagggc tgaaagggta aaagaaagaa ttgctgatat gcacaacacc atacgcaaga
      361 gtgatggtac ttcttacagc atatcgtatt cgccaaacaa taagggaatg ttaaccgatg
      421 atacacaaac acgctactat ggcgagaaat atgaaatagt agtagagcag gataaaatta
      481 ttaagctgta ctacgaaggc aaagaagtgg ctgcagatga aataccaaat cataaagaag
      541 cttgcgaccg tgtgatgcgc aatgccatga aaaaataaat tttcgtaaaa caaaataaaa
      601 acaaaccacc gtggatagag taatagaaaa aaagaaatgg cctgcatcgc gcattgcatt
      661 tattgcaggc atacttcttt ttgctgcatt tgtcctttat acttttcttt tttcctcagg
      721 aggaaaaaaa ttaaatgttg aaggcgaacg tatatccatc agcgaagtga agcaagatga
      781 ttttcgtgaa tacattcctg tgaacggact tgtgttgccg gtaacttcca tttatctcga
      841 tgcacttgaa ggtggccgcg tggaagaaaa atttgtggaa gatggtgctc aactaaaaaa
      901 aggtgatgcc attttacgtt tatcaaatac cgatttggaa ttgagtttgg ccaacgaaca
      961 aacagcagta ttcaatgtgc ttacgcaaat gcaaaacaca aaaaacaatg ccgaacaaaa
     1021 ttctatcaac cgccaaacca atatggccga tattgataat gcattgaaag aagccgaacg
     1081 gatttataac acaaacaaaa aattgtttga cgataaagtt atagcagagc aagattttaa
     1141 gtccaccgaa aatttgtaca actaccaggt tacgcgaaaa aaacttgccg aagatattat
     1201 gaaacaagat ggcaacagca tgaaacaaca agtggtacaa atgaaagaaa gttacgagcg
     1261 catgcaacac acacttgcat tgatgcagcg taaagtgggt gaccttattg ttcgtgctcc
     1321 tgtcgatgga caaataactt cgctcgatgc cgagtatgga caatcgataa acaaagggca
     1381 acgcctggga cagatagatg taatgagcgg atttaaagtg cgtgccgaaa tagatgagca
     1441 ttatataagt cgtgtgttcc ccgatttgca aggcgaaatt ttgcttggcg aaaaaacata
     1501 tcacttgaaa attaaaaaag tttattcgca agtaagcaac ggccgctttc aggttgattt
     1561 ggtttttatc gatgctgttc cacaaaatat tcgcagagga caaacacttc aaatacgcct
     1621 tgcgcttggc gatgagacta aagcattgct tgtaaacaaa ggtggcttct accaacaaac
     1681 aggaggcaac tgggctttta aattaagtgc cgatggaaaa aaagcttaca aagtagatat
     1741 gcaggttggc cgccaaaatc ccgattacta cgaaattctt tcgggcctaa aatccggtga
     1801 taaaattata acaagcagct atgaaacatt tggcgatatg gaggagttgg agataaagta
     1861 gagttgggaa agttcaatag ttcaatagtt caatagtttt gaaagttgag aaggttttga
     1921 aagttgagaa agttttcgcg aagcaatcat tgctgttcgg ctttagccaa cggaattttt
     1981 tctcctcccg tcattgtaag cgtagcgaag caatctctgc atcaataatt gcttcagtgg
     2041 tccccctttc gcaataatgg aaagatgaga aataattatt cggtattcat tcgtgcattc
     2101 cccgacactg cggtcgaggc aggcgtggca aaaa
//
LOCUS       DAORQA010000308         2125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11466_length_2125_cov_22.789372, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000308 DAORQA010000000
VERSION     DAORQA010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11466_length_2125_cov_22.789372"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PK736_09350"
     CDS             <1..202
                     /locus_tag="PK736_09350"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRC33631.1"
                     /translation="FVNQVHQTIFTICSNPNQFRNYYKDFYELGVKKFPFSLIYIINE
                     LQNEILITSIFHHKRNPKKKYK"
     gene            321..581
                     /locus_tag="PK736_09355"
     CDS             321..581
                     /locus_tag="PK736_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33632.1"
                     /translation="MKYFWLAAIIDTRIPIQNNSPDVASIGTINLLNKLYVNNQQYVF
                     YGAKVAIPITKKFGFNAGMNIAALSRNAAAALAFSASIYYKR"
     gene            804..>2125
                     /locus_tag="PK736_09360"
     CDS             804..>2125
                     /locus_tag="PK736_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRC33633.1"
                     /translation="MKAKNSFSVKKTRNAKFLQIKFSVFILCTFFFFNMQGQNNPVLN
                     FTVLNTAPINIGDTVFVQNTSIGFTQQTSFVWNDGKMCYFPINIDTAFNPALCNDTII
                     GQTQIMKFVYYQTKPFYVTLNATDTAGNFYSNGKYVIVGPICTDGNMSCENYVSNGMF
                     DHIDFCPEFGGKLGSYYNSANGQWIYEASSCWYATNNICTPDLFLTTNCILPNGTPIS
                     LNDLPGNIFYCELDHSTNALLPGCANVITGYSPNWQTNTGREYIQQKLSTPLSAGAYD
                     VSFWVSLSDWVNYASNIGMLISDTPPLYFPNPNWDVLDATPSISAISQLGGVVTNANG
                     WTQIKGVFNAVGGEEFVTLGNFGINSYLTYNGITNGQCPPTQPPFELCHYLIDDVVIT
                     KHLDVSATITQNCQPFFGSIDLTVNGLNGPYTFLWSNGAVTQDIFNLIG"
BASE COUNT          696 a          370 c          342 g          717 t
ORIGIN      
        1 ttttgtaaat caagtgcatc aaaccatttt tactatttgc tcaaatccca atcaattcag
       61 aaattattac aaagattttt acgaattagg tgtcaaaaaa tttcctttca gccttattta
      121 tatcatcaac gaattgcaaa acgaaatatt aattacatct atatttcatc ataaaagaaa
      181 ccctaaaaaa aaatataaat aattaaaaaa aatccacacc ctgcactttc actcgaacct
      241 ctcgatagaa tgcaatttcg ttttagtacc atgaataaag ttcatgctcc aaggttgatg
      301 ccgaatttgg tattaaagta ttgaaatatt tttggcttgc ggcaattatc gacacacgaa
      361 ttcctatcca aaataacagt cccgatgtag cctcaattgg cacaattaat ttactcaaca
      421 aactttatgt aaacaatcag caatatgtat tctatggtgc aaaagtggca atccctatca
      481 caaaaaaatt tggcttcaat gccggaatga acattgctgc attatcgcgt aatgccgctg
      541 cggcactcgc tttttcggct agtatttact ataagcgttg atgttgcttc aaagctaatc
      601 tcaacaatct ttaaaaatat atattattca accttgctaa ttacattaat ttaacttcat
      661 tctttaactc aactattgag gccatgtttg atgtaaattg acgataaagt tttgtgtttt
      721 attttttaaa tttgcttgcc ataccaaatt aataggctat gtttgttatg tcatttattt
      781 taatcatcta aaaacaaaaa attatgaaag ctaaaaactc gttttccgta aaaaaaactc
      841 gaaatgcaaa atttcttcaa ataaaattta gcgtttttat tttatgcact ttcttctttt
      901 tcaacatgca aggccaaaat aatccagtgt tgaattttac tgttttaaat actgcaccaa
      961 ttaacatagg tgatactgta tttgtgcaga atacttcaat tggatttaca caacaaacat
     1021 cctttgtttg gaacgatggc aaaatgtgtt atttccctat caatatagac acggcattta
     1081 atcccgcact gtgcaacgat acaataattg ggcaaacaca aattatgaaa tttgtttatt
     1141 accagacgaa accattttat gttacattaa acgctaccga tactgctggt aatttttatt
     1201 caaatggcaa atatgtaatt gtagggccta tatgcaccga tggaaacatg tcgtgcgaaa
     1261 attacgtaag taacggtatg tttgatcaca tagatttctg cccagaattc ggtggaaaat
     1321 taggtagtta ttataatagt gccaatggac agtggattta tgaagcttct tcatgctggt
     1381 atgcaacaaa taatatttgt acacctgatt tatttcttac cacaaattgt attttgccaa
     1441 atggaacacc tattagttta aatgatttgc cgggtaatat tttttattgt gaattggatc
     1501 attcaacaaa tgctcttttg cctgggtgcg cgaatgttat tactggttat agcccaaatt
     1561 ggcaaacaaa tactggccga gagtacattc aacagaagtt aagcacacct ttaagtgctg
     1621 gggcttatga tgtttctttt tgggtttcgt tgtctgattg ggttaactat gcaagtaata
     1681 ttggcatgtt aatttctgac acacctcctt tatattttcc aaatcctaac tgggatgtac
     1741 ttgatgcaac accatcaata agtgccataa gtcaattagg cggagtggtt acaaacgcaa
     1801 atggatggac ccaaataaaa ggagtattca atgctgttgg aggagaggag tttgtcactc
     1861 taggaaattt cggcattaat agttacctga catacaatgg aattaccaat gggcaatgtc
     1921 caccaacaca gcctcctttt gaattgtgtc attatctgat tgatgatgtt gttataacca
     1981 agcatttaga tgttagtgca actattaccc aaaattgcca accattcttt ggtagtatag
     2041 acctaacagt aaatggtcta aatggtccgt acactttttt atggtcgaat ggtgctgtta
     2101 cacaagatat tttcaatttg attgg
//
LOCUS       DAORQA010000309         2116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11522_length_2116_cov_4.044153, whole genome shotgun sequence.
ACCESSION   DAORQA010000309 DAORQA010000000
VERSION     DAORQA010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11522_length_2116_cov_4.044153"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PK736_09365"
     CDS             complement(<1..559)
                     /locus_tag="PK736_09365"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC33634.1"
                     /translation="MANKTKARLENEIEHGKKLSAGGAEDIWNWASPAGQVRADRRAN
                     LLTKAGNIKSTSKVLEIGCGTGLFSGKVYSATKADITAIDISEILLDEARLKNKGITF
                     QIGDAMNLDFKDNTFDAVFGSSVLHHLEMDKAVKEIYRVLKPGGRMAFAEPNMINPQI
                     MVMKNVPFVKRMLGDSPDETAIVRWK"
     gene            complement(690..1430)
                     /locus_tag="PK736_09370"
     CDS             complement(690..1430)
                     /locus_tag="PK736_09370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRC33635.1"
                     /translation="MKESFSIQAGDFLFKNAFPIYDFIYPYFKRKQDKTEIDLLKQHI
                     KPGDIVLDIGANIGFYTSILSQLAGNKGQVHAFEPDITNFKYLKRKTSHLKNITINAA
                     AVNSNGGKITVYVSPMLNVDHRTYEPENYASKYEMDAVSIDEYLQKNKKVDFIKMDIQ
                     GFEFEALKGMEKTLAFNNEIKILSEFWPYGFTKAGCDALTVAQWIWDKGFTITLMHDV
                     HQTLMNEKSIDELPVEEKKYFNIFIQRR"
     gene            complement(1444..>1827)
                     /locus_tag="PK736_09375"
     CDS             complement(1444..>1827)
                     /locus_tag="PK736_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HRC33636.1"
                     /translation="PMIKDGVKALKYFSERGIRTNCTLVFSAGQALLAAKAGATYVSP
                     FLGRLDDISSDGMQLIRDIRLIYDNYAYETLILAASIRHPMHIIDCAKIGADVVTTPL
                     NVITDLLKHPLTDIGLAKFLADHNK"
     assembly_gap    1828..1899
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1900..2102)
                     /locus_tag="PK736_09380"
     CDS             complement(<1900..2102)
                     /locus_tag="PK736_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HRC33637.1"
                     /translation="MLFFIDTANLAQIKEAHDMGVLDGVTTNPSLMAKEGIKGKDKVL
                     KHYVDICNIVNADVSAEVIATDY"
BASE COUNT          571 a          419 c          366 g          688 t
ORIGIN      
        1 atttccatcg tacaattgca gtttcatcgg gcgagtcgcc aagcatacgt tttacaaacg
       61 gcacattttt catcaccatt atttgggggt taatcatatt aggttcagca aaagccatgc
      121 ggccaccggg ttttaacaca cggtaaattt cttttacggc cttgtccatt tccaaatgat
      181 gcaatacact gctgccgaat actgcatcaa aagtattgtc cttaaaatct aaattcatag
      241 catcgccaat ttgaaaagta atgcccttgt tttttagtcg tgcttcgtca agtaatattt
      301 ccgaaatatc gatagcagta atatcggcct tggttgcaga ataaacttta ccgctaaaca
      361 aacctgtacc gcatcctatt tcgagcacct tgcttgtgct ttttatgttg ccggctttgg
      421 tcaataaatt ggctctacgg tcggcacgta cttgccccgc tggcgatgcc cagttccaaa
      481 tatcttcggc accacctgcc gacaattttt tgccgtgttc tatttcgttt tctaaacgcg
      541 ctttggtttt attagccatt atatactttg taattttatt tggtcaaaaa tataaacacc
      601 ttctgtatac gtatgtatac ttgccaaaag ttttaaacat taaattttat gaaacagtca
      661 tcaacatcat gggccataat gttggattgc tacctccttt gaataaaaat attgaagtat
      721 tttttttcct caaccggcaa ttcatctatg cttttttcat tcatcaatgt ttgatgaaca
      781 tcgtgcatta atgtgatagt aaatccttta tcccaaatcc actgtgcaac ggtaagcgca
      841 tcgcagcctg ccttggtaaa tccgtagggc caaaattcac tgagtatttt tatttcatta
      901 ttaaaagcca aagttttttc catccctttc aaggcttcga actcgaatcc ctgaatatcc
      961 atttttataa aatctacttt tttgtttttt tgcaaatact catctatact tacagcatcc
     1021 atttcatatt tcgatgcata attttcaggc tcataagtgc gatggtctac attcaacatg
     1081 ggcgatacat acactgttat ttttcctcca ttactattta ctgctgcggc attgatagtt
     1141 atgtttttca aatgcgaagt cttgcgtttc aaatatttga aattggtaat atcaggttca
     1201 aaagcatgca cttgaccttt gttgcctgca agttgcgaaa gtatacttgt gtaaaagcca
     1261 atattggcgc caatatcgag cacaatatcg cccggcttta tgtgctgttt taataaatcg
     1321 atttcggttt tgtcttgctt gcgcttaaag taaggataga taaaatcgta aatgggaaaa
     1381 gcatttttga agagaaaatc tccggcttgt atggaaaaag actctttcat ttacaattaa
     1441 aaattacttg ttgtggtcgg ccaaaaattt cgccaaacct atatctgtga gcgggtgctt
     1501 tagcaaatct gttattacat ttagtggagt agtaactaca tcggcaccaa tttttgcaca
     1561 atcaataatg tgcatagggt ggcgtataga tgcagcaagt attaaagttt cgtatgcata
     1621 gttgtcgtat atcaatctta tatcgcgaat aagttgcatt ccatcgctcg atatatcatc
     1681 taagcgacca agaaatggcg aaacgtaagt agcaccggcc tttgcagcca gcaaggcttg
     1741 cccagccgaa aacaccaatg tacagtttgt gcgtatgcca cgttccgaaa aatattttaa
     1801 tgcctttaca ccatctttta tcatgggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cgtagtcggt ggcaatcact
     1921 tcggcactta catcagcatt tacaatattg caaatatcaa cataatgttt cagtactttg
     1981 tctttgcctt tgatgccttc tttggccatc agcgaagggt tggtggttac gccatcgaga
     2041 acgcccatat cgtgggcttc tttaatttgt gcaaggtttg cggtgtcgat aaagaataac
     2101 ataatctata taaagt
//
LOCUS       DAORQA010000310         2108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11578_length_2108_cov_5.615197, whole genome shotgun sequence.
ACCESSION   DAORQA010000310 DAORQA010000000
VERSION     DAORQA010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11578_length_2108_cov_5.615197"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2037
                     /locus_tag="PK736_09385"
     CDS             <1..2037
                     /locus_tag="PK736_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33638.1"
                     /translation="LNMVAAIITLAAIGFKSQAQVAALPFTASLDSFYEITGTTVDAL
                     YADDVVYQNIPIGFDFYLGGTLHDKMSINTNGYIELDSTGTSTFINIMNGSQNNLVAP
                     FAADLQNKSSNASLQYLTVGTAPNRVCYIQWLHYSYFAGQGDINFQIRLYESSNCISF
                     VYGANTLVQNPLQTQIGLRGTTNLDFVVLGDTSCNWASAYPYPTITTYFPVSFSCNMP
                     SGFAFNFGSCINKGTANFGFITGKVFDDTNGNGTLDSAEAGIKNQLVNITPGNYYVST
                     NATGSYSFFFVDSTITYSLTVAPITYWNQTNIPNVITCQPSTQACSGLNFGFQIIPNI
                     HEVEIHCPNWGAKPLQPEPMPIWYVNNGTATESDTITFEMDSLYSFISAIPAPTSVNG
                     QTIKWAYSNLMPNQSGSIMLHLLPSGQAVLGNYLDSKLSIAPLNDTIPTNNIINLHQL
                     ITNSWDPNDKLAEPSGMIKAGTEIDYTIHFQNTGNAAADNVVLKDTLDSNLDLMSFKL
                     IGASHNVNFAMEGNGIATFTFYNIQLPDSGTDMAGSNGYVSYKVKTKQGLAPLTVINN
                     TAGIYFDFNPAIITNMTADTIEIPLGIITTTEKVFVIHASPNPSVGNIVFAFSKNINE
                     QANLTITDANGKIILQQNNITSTQNIDLKNLAAGIYIATISSQRGTETVKIVKE"
BASE COUNT          689 a          434 c          369 g          616 t
ORIGIN      
        1 cttaacatgg tagctgccat cattacttta gctgccatcg gttttaaatc gcaggcacaa
       61 gttgctgcct tgccattcac tgcatcgctc gattcatttt atgaaataac aggtacaaca
      121 gtagatgctt tgtatgccga tgatgttgtt tatcaaaaca ttcccatagg tttcgatttt
      181 tatttgggcg gcaccctgca cgataaaatg tcgataaata ccaatggtta cattgagctc
      241 gacagcaccg gtacaagtac gtttataaat attatgaatg gctcgcaaaa taatttagtt
      301 gctccatttg ctgccgactt acaaaacaaa agcagcaatg caagtctgca atatttaacc
      361 gtaggaacag cacctaacag agtgtgttat attcagtggt tgcactactc gtactttgca
      421 gggcagggcg atattaattt tcaaattcgg ttatacgaat cgtcaaattg tatcagcttt
      481 gtttacggtg caaacacatt ggttcaaaac ccacttcaaa cacaaattgg tttgcgtggc
      541 actactaatc tcgattttgt tgtgcttggc gatacctctt gcaattgggc aagtgcttat
      601 ccttatccaa caattactac ttattttcct gtaagttttt cgtgcaatat gccatcgggt
      661 tttgcattta attttggctc gtgcatcaat aaaggcaccg ctaattttgg ttttataaca
      721 ggtaaagttt ttgacgatac caacggcaat ggcacactcg actcagccga ggcgggaata
      781 aaaaatcaat tagtaaatat tactcccggt aactattatg tatctaccaa tgccacagga
      841 agttattctt ttttctttgt tgattctacc attacatact cgctcaccgt agcaccaatt
      901 acttattgga accaaacaaa tattccaaat gtaataactt gtcagccatc aacacaagca
      961 tgcagcggat tgaattttgg ttttcaaatt attccaaata tacatgaggt agaaatacat
     1021 tgccctaact ggggcgccaa accgttgcaa ccagaaccaa tgccaatatg gtatgtaaat
     1081 aatggcacag caacagaaag cgataccatt acgttcgaaa tggattcgct ctattcattt
     1141 attagtgcca taccagcacc tacttctgta aacggacaaa ctataaaatg ggcgtatagt
     1201 aatttaatgc caaaccaaag tggcagtatc atgttgcact tgttgccttc gggccaagcg
     1261 gtattaggca attatcttga ttctaaattg agcatagctc cattgaatga taccattcct
     1321 acaaacaaca ttataaattt acatcagtta attaccaact cgtgggatcc caacgataaa
     1381 cttgccgagc catcgggtat gataaaagcc ggaaccgaaa ttgactacac cattcatttt
     1441 caaaacaccg gaaatgctgc tgccgataat gtagtgctaa aagatacgct cgattcaaat
     1501 ttagatttaa tgtccttcaa actaataggc gcatcacaca atgtaaattt tgcaatggag
     1561 ggaaatggca ttgcaacgtt tactttttac aatattcaat tgcccgatag cggcaccgat
     1621 atggccggaa gcaatggtta tgtatcgtac aaagtgaaaa ccaaacaagg ccttgcacca
     1681 ttaactgtta taaataatac tgccggcatt tatttcgatt ttaacccggc aataattacc
     1741 aacatgacag ccgatactat cgaaattcca cttggtataa ttaccaccac cgaaaaagtt
     1801 tttgtaatac atgcatcgcc aaatccatcg gttggcaata tagtttttgc attttcgaaa
     1861 aacataaatg agcaagccaa cctcaccata accgatgcta atggaaaaat aattttacag
     1921 caaaacaaca ttacatcgac acaaaatatc gatttgaaaa atcttgctgc cggtatttac
     1981 attgccacta taagttcaca acgcggcacc gaaacagtga aaattgtgaa ggagtaaatt
     2041 taatggaata aataatttgt agaaaaaata cctaattaaa tttacaaaag cctgataagt
     2101 gataaatg
//
LOCUS       DAORQA010000311         2102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11619_length_2102_cov_19.909624, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000311 DAORQA010000000
VERSION     DAORQA010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11619_length_2102_cov_19.909624"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PK736_09390"
     CDS             complement(<1..1016)
                     /locus_tag="PK736_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33639.1"
                     /translation="MSSTEKIIANTIKEKILPGGISLTVKLLSPNKNVSSILKDQKIN
                     ASYTDTVNIERSLQSLLVKLYDEGYYTASIDTIEYAEKKVIATLNAGPAFAYFILNKN
                     ANMAFADKQLRGDEVLKAGQLKKYFSSILQQLENNGYPFAQVQLVNIKMRDSIIQGDV
                     SITRNEKIIIDSLIVNGNARISNKFLEQYLNIKAGNVYDERLIRGISRKLKNLNFIRE
                     TKAVQVTFTKTLCRINVFVESKRASNADGIVGILPPAKAGDKTIVTGDLKLLLSNSAG
                     RGEIIDINWRQPSAKTQRFSGIVSYPYLFHSPVGIDGKIEIFKKDTTFLDVIKVLGVN
                     YILGA"
     gene            complement(1136..1831)
                     /gene="ung"
                     /locus_tag="PK736_09395"
     CDS             complement(1136..1831)
                     /gene="ung"
                     /locus_tag="PK736_09395"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRC33640.1"
                     /translation="MTDNKPNNKTIDIEASWYNALQDEFGKEYFTQLRAFLREEKNKG
                     LRIFPPGKQIFSAFNYTPFDKVKVVIIGQDPYHGEGQANGLCFSVADGIKQPPSLQNI
                     FKELNVDVGMEKPTTGNLEPWAKQGVFLLNAILTVRANEPASHSKAGWQQFTDAVIST
                     ISKKREGVIFLLWGNFAKQKAQLIDSTKHHILTAAHPSPLAGGAFFGCKHFSATNNIL
                     IKHGLPPINWALK"
     gene            complement(1851..>2102)
                     /locus_tag="PK736_09400"
     CDS             complement(1851..>2102)
                     /locus_tag="PK736_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33641.1"
                     /translation="KGGVSFGLDNFASPIMNFKSEYKGDDFTSVSDNKILFFGARISL
                     DYRFGKMEFKNSEKKKRIKNDDLKQGEGDGMGGGMGGGK"
BASE COUNT          577 a          422 c          386 g          717 t
ORIGIN      
        1 gctccgagaa tataattaac gcccagtact ttaattacat caagaaaggt ggtgtccttt
       61 ttaaatattt caattttacc atctatacct accggagaat ggaataaata agggtaactc
      121 acaatgccgc taaaacgttg tgtttttgca cttggttgcc gccagtttat atcaatgatt
      181 tcgccacgcc cggctgagtt gcttagtaat aattttaaat ctcctgtaac aatagtttta
      241 tcacctgctt ttgccggggg aagtattcct acaataccat ctgcattact cgctcgctta
      301 ctctctacaa aaacatttat ccggcacaat gtttttgtaa aagttacctg caccgctttt
      361 gtttcgcgaa taaaattaag gttttttagt ttgcgcgaaa tcccgcgaat caatctttcg
      421 tcatacacat tgccggcttt gatattgagg tattgctcca aaaacttatt gcttatacgt
      481 gcattaccat ttactatcag cgaatcgatt attatttttt cgttgcgggt aatagaaaca
      541 tcaccttgta tgatactatc gcgcatttta atatttacca gttgcacctg agcaaacgga
      601 tagccgttgt tttcgagttg ctgtaatatc gaggaaaaat attttttcaa ctgccctgct
      661 tttagcactt catcgccacg cagttgttta tctgcaaacg ccatgttcgc atttttgttc
      721 aatataaaat atgcaaaggc cggtcctgca tttaaagtcg ctatgacttt tttttcagcg
      781 tattcgatag tatctataga ggcagtgtaa tacccttcgt catatagttt cactaagagg
      841 ctttgcaaac ttcgttcgat attaacggta tcggtatacg atgcatttat tttttggtct
      901 ttaagaattg atgaaacatt tttgttgggc gatagcaatt ttaccgtaag cgaaattccg
      961 ccaggaagta ttttttcctt aatggtattc gcaattattt tttcggttga actcatattt
     1021 ccgatcaaag gaatgacatt ttgcgccaca gaaactgtac aaagcattgt cagaaagcaa
     1081 agagaaagca catgcttgaa ggccaataga attgaaatat tttttttttg cgcaatcatt
     1141 ttaatgccca gtttattggc ggtaatccat gcttgatcaa aatgttattt gtagccgaaa
     1201 aatgcttgca gccaaagaaa gccccacctg caagtggtga cggatgtgcc gcagtaagga
     1261 tgtgatgttt ggttgaatcg ataagttgtg ctttttgttt ggcaaagttt ccccacaata
     1321 aaaaaataac gccttcgcgc tttttcgaaa tagtagaaat aacggcatcg gtaaattgtt
     1381 gccaaccggc tttgctgtgt gaggcaggtt cattggcacg cacagtgaga atagcgttga
     1441 gtaaaaacac tccttgtttt gcccatggtt caagattgcc tgttgttggt ttttccatgc
     1501 ctacatcaac gttgagttct ttaaaaatat tttgcagcga tggtggttgt ttaattccat
     1561 cagccaccga aaagcataac ccgtttgcct gtccttcgcc atgatatggg tcttggccta
     1621 tgattaccac ttttacttta tcgaacggag tataattgaa ggcggaaaat atttgtttac
     1681 ccggaggaaa aattcgaagg cctttatttt tttcttcacg tagaaacgcc cttagttggg
     1741 tgaaatattc tttaccgaat tcatcctgca aggcattgta ccacgatgct tcaatatcta
     1801 tggttttatt atttggtttg ttgtctgtca aaatattttt ttaaaataaa ctattttcct
     1861 ccgcccatgc ctccgcccat accatcgcct tcgccttgtt tgaggtcgtc attttttatg
     1921 cgctttttct tttcgctatt tttaaattcc attttgccaa agcgataatc taaactaata
     1981 cgggcaccaa agaataaaat tttattgtcg ctcaccgaag taaaatcatc acctttatat
     2041 tcgcttttaa aattcatgat gggcgaagca aaattatcga gcccaaaact cacaccacct
     2101 tt
//
LOCUS       DAORQA010000312         2101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11631_length_2101_cov_6.435973, whole genome shotgun sequence.
ACCESSION   DAORQA010000312 DAORQA010000000
VERSION     DAORQA010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11631_length_2101_cov_6.435973"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..1219
                     /gene="trxB"
                     /locus_tag="PK736_09405"
     CDS             269..1219
                     /gene="trxB"
                     /locus_tag="PK736_09405"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRC33642.1"
                     /translation="MMSEIEKVNCLIIGSGPAGYTAAIYAARADMKPVMIAGLQPGGQ
                     LTITTEVENYPGYPEGTQGPEMMEDFRKQAQRFGTDVRWGYVSAIDLSSKKPPYMVTV
                     DEKHKIEAQSVIISTGASAKWLGLESEKKFNGFGVSACAVCDGFFFKKQDVAIVGAGD
                     TAAEEATYLAKLCNKVYMIVRKDEMRASKAMQHRVMNTPNIEILYNHETVEILGDQAV
                     TGATMLNNKTNEKVHLNVTGFFIAIGHQPNTDLFKGQLDLDTDGYIITQPGTSRTNVE
                     GVFAAGDVQDKTYRQAVTAAGTGCMAALDAERYLAAKGVH"
     assembly_gap    1478..1487
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          664 a          361 c          429 g          637 t
ORIGIN      
        1 tcggccttag gtaataattt gctattgtgg ttttcaatca gcactatttt gttttatttt
       61 taaaagtaag catctattgt tattgaaatg aattgctgca aacaacaatt tagtaaaacg
      121 taaagcgatt atttgaggca caaaaaaaag aagtactttt gtgctcccaa tttatttgtt
      181 tggtaagcac aaataagata agggcggtca ttgggccgct tttattgttt ggttaagtat
      241 tgtgtattaa ttttaaaaaa aaggaaatat tatgtcagaa atagaaaaag taaattgcct
      301 tattataggc agtggcccag cgggttatac tgcagccata tatgcagcac gtgccgatat
      361 gaaaccggtt atgatagccg gactgcagcc tggtggccag ctcacaatca ctaccgaagt
      421 tgaaaactat cccggttatc ccgaaggcac acaaggcccc gaaatgatgg aagattttcg
      481 taagcaggct caacgctttg gcaccgatgt gcgatggggt tatgttagcg ccattgattt
      541 aagttcgaag aaacctccgt atatggttac cgttgatgag aagcataaaa ttgaagcgca
      601 atcggtaatc atttctactg gagcatctgc aaaatggtta ggattggagt cggaaaaaaa
      661 atttaatggc tttggtgttt cggcatgtgc tgtttgcgat ggtttctttt tcaaaaaaca
      721 agatgttgcc attgtagggg caggtgatac tgctgccgaa gaagcaacat accttgccaa
      781 gttatgcaac aaagtgtata tgattgtgcg taaagacgaa atgcgcgctt caaaagccat
      841 gcagcaccgt gttatgaata cacccaatat agaaatattg tacaatcatg aaacagtaga
      901 aattcttggc gaccaagcgg taaccggagc aactatgttg aacaacaaaa ccaacgaaaa
      961 agtacatctt aatgttaccg gattttttat tgctatcggc caccaaccca atactgactt
     1021 atttaaggga caattggatt tagataccga tggttatatc ataacccaac cgggtacttc
     1081 acgcacaaat gtcgagggtg tttttgcagc aggcgatgtg caggataaaa cttaccggca
     1141 agcagtgact gcagcaggaa ctggatgcat ggccgcatta gatgcagagc gttatttagc
     1201 agcaaaaggt gtacattaat tttagttctt agtttttttt agttcttagt tttttagttc
     1261 ttagttctta gttcttagtt cttagttctt agtttttttt agttcttggt ttctttgtaa
     1321 ttggtttgtt ggttcttgtt ttttttgttt tggttctttg ttcctaattc ttatttttta
     1381 gttcttgctt acagttttta ttgttatttg aattagcttt tcttttacga aaactaattt
     1441 cgaaataatg ataggctgat atcaaaaagt atgtcgannn nnnnnnntaa cttggattaa
     1501 aaaaaacacg aaaatttttg ttggaaaaca atgtactcat caaaataaaa tatgtaagaa
     1561 gagttgctgc gactgtaaaa atacatcgga agaaaaacat tcttaggcaa attagataac
     1621 tacttaccaa actcaatcca aggaagaata acatggttca acgaaaacat aactaaaaaa
     1681 gtttgcaccc cgtccgaagt gtcttgacta agaacaaacc tacttgtaaa cgaagtaaag
     1741 tgctgcgcag agattgcttc accactcgca atgacgaact aagagtagtc ctctccacaa
     1801 acggaacata gagctggaaa tttggctcat agcaaactta acaaacaact aagaacttgg
     1861 cttaacgcaa acgttactaa gaactgggct tatcgcaaac gtaactatga actcggctct
     1921 cagcaaacga aactaagaac aaaaattatt tgcttttccc tacatgcttt agatgcgaaa
     1981 tgtgcgaagc tatagctgtg cgaacacggg cgtattcgat ataaggcaca ttatattcca
     2041 ggcatacttg tttcacaatt tggctaatgg ccgggtagtg tacatgtgaa atacttggga
     2101 a
//
LOCUS       DAORQA010000313         2092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11674_length_2092_cov_8.257732, whole genome shotgun sequence.
ACCESSION   DAORQA010000313 DAORQA010000000
VERSION     DAORQA010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11674_length_2092_cov_8.257732"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..582
                     /locus_tag="PK736_09410"
     CDS             211..582
                     /locus_tag="PK736_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC33643.1"
                     /translation="MNLNQVTVAVNNIEQSINFYQKLGLTLIVKSPHYARFECPKGDA
                     TFSIIQNENIAKENATHIYFECDDLDNRVVELTKKGIEFIELPNDKTWLWREAHLNDL
                     DGNHLILYYAGVNRKNPPWRI"
     gene            complement(630..1562)
                     /locus_tag="PK736_09415"
     CDS             complement(630..1562)
                     /locus_tag="PK736_09415"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HRC33644.1"
                     /translation="MKNLIKAFCVGSLLISAFQVHAQDDLTKMLDEQPQEKQKVLATF
                     KTTKLINSQTNETVHKRVLDFRVGHRFGNIGGNSGGGVHTLYGLDQSADIRIAFDYGI
                     TERLTVGFSRSKVKENLEGSAKFRILEQTTNNKMPIALTWFSNMAFTPESNDDIYLVS
                     ENGSLKNRDVRRLSYTHQLIIARKFSSAFSFQLVPTLMHRNYSPDYKDDNNIMALGGA
                     ARIKVTRSMTVIADYFYVMNKYHRTVKNADGNLMYNDPLGIGIELETGGHVFSLMFTN
                     ASSIIETEMLANTTDSWGKGGFKFSFNISRNFSL"
     gene            complement(1598..>2092)
                     /locus_tag="PK736_09420"
     CDS             complement(1598..>2092)
                     /locus_tag="PK736_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRC33645.1"
                     /translation="QNIYMTSGGKASFFSATPVEDIDAHSSSLNCILNTATNDVVYSV
                     LMTSFKFVKPLMQEHFNEKYVESDKYPKAVFKGKINEKIDYTKDGTYDITATGNLEVH
                     GVAKPYTEKGKLTIKDGVISIEGSFDVKLVDHKIEVPTLVATNIAETINIKHAATLAV
                     FKKK"
BASE COUNT          641 a          381 c          385 g          685 t
ORIGIN      
        1 ataatatccg taagtattga acaactcttc tatatatttt tcgttttgtt ttttcgtgcc
       61 atatacattt atagagttaa ggtgtcattt tactgcttat gatttttttt ggataatgct
      121 gcatacaaaa aagactaaaa aaaagtacat aaggcaaatc atttttccca aaccaatcat
      181 ttcattagca ttgctgttta aaattaaaaa atgaatctca atcaagtaac tgttgctgta
      241 aacaatatag aacaatctat taatttttat caaaaacttg gactaacact tatagtaaaa
      301 tcgccacact acgcccgttt cgaatgtcct aaaggcgatg ctacattttc gattatacaa
      361 aatgaaaaca ttgcaaagga aaacgcaaca catatctatt tcgaatgtga tgatctcgat
      421 aatcgcgttg ttgaattaac aaaaaaagga atagaattta ttgaattgcc aaatgataaa
      481 acctggctat ggcgcgaagc acatttaaac gatttggatg gcaatcacct cattttatat
      541 tatgccggag tgaatagaaa aaatcctcca tggagaattt aatgggaatt tatttttaca
      601 ggcaagtgtg atttcccaat gagcaatttt tacaaactaa aattgcgcga aatattgaag
      661 ctgaatttaa agcctccttt gccccaactg tcggttgtat tggcaagcat ttcggtttcg
      721 ataatggacg aggcattggt aaacataagg ctaaaaacat gtccgcccgt ttcgagttca
      781 atgccaattc caagtggatc attatacatc aaatttccgt ctgcgttttt caccgtgcga
      841 tgatatttat tcatcacata aaaatagtcg gcaattacag tcatactgcg tgtaaccttt
      901 atgcgcgctg caccacccaa cgccataatg ttgttgtcgt ccttatagtc gggcgaataa
      961 ttgcggtgca ttaaggttgg tactaattga aatgaaaatg cactggaaaa ctttcgtgca
     1021 ataattaatt gatgtgtgta tgaaagtctg cgcacatcac gattttttag tgagccattt
     1081 tcggaaacaa ggtaaatatc atcgttgctt tcgggagtaa atgccatgtt cgaaaaccag
     1141 gtgagtgcaa ttggcatttt gttatttgta gtttgttcaa ggatgcgaaa ctttgccgac
     1201 ccttcaagat tctcttttac cttggagcga gagaaaccaa ctgtgagtcg ctcggtaata
     1261 ccgtaatcaa aagctatgcg tatgtcggca ctttggtcca aaccatacaa tgtatgaact
     1321 ccacctccac tattgccacc tatattgcca aacctgtgtc caacgcgaaa atcgagtacg
     1381 cgcttatgca cagtttcgtt ggtttgcgag tttataagtt ttgtggtttt aaaagtagca
     1441 agcacttttt gtttttcctg aggttgttca tcgagcattt tagtaagatc gtcctgtgcg
     1501 tgaacctgaa atgcgctgat gagtaatgag ccaacacaaa atgcttttat taaatttttc
     1561 atatcgatta ggggtattaa aaatgtgata gttaatttta ttttttctta aacactgcaa
     1621 gggtagcagc atgcttaatg tttattgttt cggcaatgtt tgtggcaacc aatgtaggta
     1681 cttctatttt gtggtcaaca agttttacat caaacgaacc ttcgatagaa ataacgccat
     1741 cttttattgt gagcttaccc ttttcggtat aaggcttggc aacaccatgc acctcgagat
     1801 ttccagtagc agtgatgtca taagttccat ctttggtata atctattttc tcgtttattt
     1861 ttcccttgaa aactgctttg ggatatttat cactctccac atatttttcg ttgaagtgtt
     1921 cctgcatcag cggctttaca aatttaaaag aagtcatcaa cacagaatat acaacatcgt
     1981 tggttgcagt attaagtata caatttaaag atgaggaatg tgcatcaata tcctcgacag
     2041 gcgttgccga aaagaaagaa gctttgccgc cacttgtcat ataaatgttt tg
//
LOCUS       DAORQA010000314         2075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11765_length_2075_cov_4.411881, whole genome shotgun sequence.
ACCESSION   DAORQA010000314 DAORQA010000000
VERSION     DAORQA010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11765_length_2075_cov_4.411881"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    278..336
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<337..817)
                     /locus_tag="PK736_09425"
     CDS             complement(<337..817)
                     /locus_tag="PK736_09425"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HRC33646.1"
                     /translation="MNYLAHIFLSDNDNELMIGNFIADHIKPKERAKFSPQINAGIKL
                     HYAIDTFTDNHNVVLKSKERLRPHLHKYAPVVVDVIYDHFLAKYWHLYHHQDLLEYTI
                     KFYNMLHAHRELLPDKTKYILNYMEPRNWLYNYQHLHELEKIFGGMGRRANFDNNMAL
                     "
     gene            complement(1064..1900)
                     /locus_tag="PK736_09430"
     CDS             complement(1064..1900)
                     /locus_tag="PK736_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33647.1"
                     /translation="MKKHFFRLMALTSIVALTFSACRKNEEADNDTTAGEANAEAERV
                     YDDVDRSISEAACMSKYLDNTGGNMMNTTCPTITIDYPDSTTWPKTVTIDFGSTNCTG
                     WGNIPRRGKIISVFTGKFRNPGTVITTTFDNYYVNDNHVEGTKTITNLGTNGAGNLYY
                     SVVVSNAKITRTDGTFMTWNSTRTREWTQGSNTIGNTTDDIFLITGSGSGTNFGGNAF
                     TVNITSPLQVQRGCKWIESGVIEITPANKPMRTIDFGNGNCDNQATITIKNKTYTITL
                     KG"
BASE COUNT          602 a          341 c          389 g          684 t
ORIGIN      
        1 accaatttta ttgacacagg ctttttgttt tgaacaaaac cgaggttgat aatttatttg
       61 gtaaaatttt tttgaaagta taaaattttg attaatggga tgtaccccaa cgtgcaaaat
      121 tggcaagtaa aattcgttaa ccatgcttaa cagtgtgttt acaatttcga aacattaaaa
      181 aacaagggta caaaaaacca aaatgcattt ttgcaataaa aagaaattaa caattgcact
      241 tttggcacaa tttatttttt gtacccatga cagaaatnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntaag agccatatta ttgtcgaaat
      361 tggctctgcg ccccattccg ccaaatattt tttccaactc atgcaaatgt tgatagttat
      421 aaagccaatt gcgcggctcc atatagttga gtatatattt ggttttgtcg ggcaatagtt
      481 cacgatgggc atgcagcata ttatagaact ttatagtgta ttccaataaa tcctggtgat
      541 ggtataagtg ccaatatttt gcaagaaaat gatcgtagat tacatccacc accaccgggg
      601 catacttatg caaatgaggg cgcaatcgct ctttactttt caataccacg ttatgattat
      661 cggtaaatgt atcgatggca taatgcaatt taattccggc atttatttgt ggtgaaaatt
      721 ttgcccgctc ctttggcttt atatgatcgg caataaaatt tccaatcatc aattcgttat
      781 cattatcgga tagaaatatg tgtgctaaat aattcatttg ttgttgacaa acatacgtaa
      841 aataggatgt agaaaaatat caaggtttgt gggacgaatt attcaaggtt tgtggggaca
      901 caaaccttgg aggatagttg aaattaaaaa ggtttttggg gacacaaacc ttggagacag
      961 gggacacaaa ccttggacga aaaaaaaatg ccacaagtat aaacctgtgg catttttaaa
     1021 atataacgaa gcttctttat tgaaacttat ttcacaaaaa catttatcct ttcagcgtga
     1081 tagtatatgt tttgtttttt atagtaatgg ttgcctgatt atcgcaattg ccatttccaa
     1141 aatcgatggt gcgcattggt ttgtttgcag gagtaatttc aatcactcca ctttcaatcc
     1201 atttacaacc gcgctgcacc tgtagtggcg aagtaatatt tacggtaaat gcattgccgc
     1261 caaaatttgt tccgctacca cttccggtaa ttaagaaaat atcatcggta gtattaccaa
     1321 ttgtgttgct accttgtgtc cactcacgtg tacgtgtcga attccatgtc ataaatgtgc
     1381 catcggtacg ggtgattttt gcattgctca caactacact atagtataaa ttgccggcac
     1441 catttgttcc caaattagta attgtctttg tgccttccac atggttatca tttacatagt
     1501 agttgtcgaa tgtggtggta ataactgttc cgggattgcg gaacttacct gtaaatacag
     1561 aaattatttt tcctctgcgt ggaatattac cccagcctgt gcagttggtt gagccaaaat
     1621 cgatggtaac agtttttggc catgtggtgc tatcaggata gtcgatggtg atggttggac
     1681 aagtggtgtt catcatgttt cctccggtat tatcgaggta tttgctcata catgcagctt
     1741 cgctaatgga gcggtctaca tcatcgtaaa ctctttcggc ttcggcattt gcttcgcctg
     1801 ctgtggtgtc gttatcggct tcttcgtttt tgcggcaagc cgaaaatgtt aatgcaacta
     1861 ttgatgtgag tgccatcaat ctaaaaaaat gttttttcat attatttgtt tttaaaattg
     1921 aactacggac ttatgacaat gtgcgtttaa aaaggtttaa aatttttatt tattattttt
     1981 atgcaataat tttttgaaag ttgcagatga tgtttaatgt tgggttcaac ggtttataaa
     2041 aagatatact acacgcagtt aatattgaag agaat
//
LOCUS       DAORQA010000315         2070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11785_length_2070_cov_9.411911, whole genome shotgun sequence.
ACCESSION   DAORQA010000315 DAORQA010000000
VERSION     DAORQA010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11785_length_2070_cov_9.411911"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PK736_09435"
     CDS             complement(<1..705)
                     /locus_tag="PK736_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRC33648.1"
                     /translation="MIKANLYLIPCSLSDDYAIDQIPASTLNIIKTIKYFIVEREKSA
                     RKFLKHCESQYEQSEFVFLEIDKHNSEQSYTAFLQYCLQGNDVGLISESGVPAVADPG
                     AKVVEQAHQLHIRVVPMVGPSSILLALMASGLNGQQFCFNGYLPKDKLALQQKIKTLE
                     KESQIKNQTQIFIETNYRNEQTFQAMLQSLQPSTKLCVATNITLPHEKIRTLTVAEWK
                     KTSIKLQDQYCVFCFLA"
     gene            complement(710..>1185)
                     /locus_tag="PK736_09440"
     CDS             complement(710..>1185)
                     /locus_tag="PK736_09440"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRC33649.1"
                     /translation="MDIKILMICLGNICRSPMAEGIMKAKIKEHKLKWKVDSAGTSSW
                     HAGDKPDKRAIAISKANGVDISDQRSRAFTPADFSEYNVIFVMDESNQSDIVEMALTE
                     RQGKRVKLLLEYAEMDMKSVPDPFYGEEKGFQATFDLLNEACEKALLKLKAEYAD"
     assembly_gap    1186..1213
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1404..>2070)
                     /locus_tag="PK736_09445"
     CDS             complement(1404..>2070)
                     /locus_tag="PK736_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33650.1"
                     /translation="STGNPNMWQWYFPGASPDTSTMQNPAGIYFPSYGTYPVTLVAYN
                     SFGQDSLTITSFITVVANPPKPIVTFNGSQICCSQAQSYQWYFNNAPITIGGTSACYT
                     PSLLGNYYVIITDSNGCSSPSDVFIVNGINENALNSLGYNLSPNPANNILYITNSIHN
                     MANVTITIYDASLRTIKEINTNNTFNQKQINIPCDDLSDGIYFIRINEQKHFGVEKFI
                     VRH"
BASE COUNT          596 a          382 c          388 g          676 t
ORIGIN      
        1 tgccaaaaaa cagaatacac aatactggtc ttgcaatttg atggaagttt ttttccattc
       61 tgctactgtt aatgttctta ttttttcgtg gggtaatgtg atattggtgg ccacacataa
      121 ttttgtagat ggttgtaatg attgtaacat tgcctgaaaa gtttgctcgt tgcggtagtt
      181 tgtttcaata aaaatttgcg tttgattttt tatctgcgat tccttttcaa gcgtttttat
      241 tttttgttgc aatgccagtt tatcttttgg caaatatcca ttgaaacaaa attgctgacc
      301 atttaaaccc gaagccatta aagcgagtag gatagaagat gggccaacca ttggcacaac
      361 ccttatatgt aattgatgtg cttgttctac aacttttgca ccggggtcgg caactgcagg
      421 cacgcccgat tccgagatta atccaacatc gttgccctgc aagcaatatt gtaaaaaggc
      481 agtatacgat tgttcactgt tgtgtttgtc aatttctaaa aaaacaaatt cgctttgttc
      541 gtattgtgat tcgcaatgct ttaaaaattt tcgtgccgat ttttcgcgtt caacaataaa
      601 atattttatc gttttaataa tattgagggt cgatgccggt atctggtcta ttgcataatc
      661 atcgctgaga ctgcatggta taaggtatag gtttgcttta atcattttat taatcagcgt
      721 attctgcttt aagtttcagc aaagcttttt cgcaagcttc gtttagtaaa tcgaacgtgg
      781 cctgaaaacc tttttcttca ccataaaatg gatcgggaac acttttcata tccatttcgg
      841 catattcaag caaaagcttc acacgtttac cctgccgttc agttaacgcc atttctacta
      901 tatcgctttg gttcgattcg tccattacaa aaataacatt gtactccgaa aagtcggctg
      961 gagtaaatgc ccggctgcgt tgatcactta tatccactcc attggctttg ctgattgcaa
     1021 tagcacgctt atcgggctta tcgcccgcat gccaagatga tgttccggcc gaatcgacct
     1081 tccacttgag tttgtgttct tttatttttg ctttcattat accctcggcc ataggcgagc
     1141 ggcatatatt tcccaaacat atcattagta tcttaatatc cataannnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnncgcaaat atatataacc cttaatggat aactaataaa gcggtgatga
     1261 aaaaatgatt gtttgatcaa ggatggcttt tgtttgataa tccaacaatg agtccgcccc
     1321 gaaaaaataa tttttgccac caaaactcta aggcacaaaa tttcactaag tcggaaaaaa
     1381 ataattccga aaaatatttt ttctcaatgc cgcacaataa acttctccac accaaaatgt
     1441 ttttgttcgt ttattctgat gaaataaatc ccatctgata aatcatcaca aggaatatta
     1501 atttgttttt gattgaaagt attattggta tttatctctt tgatggttcg cagtgaggca
     1561 tcgtatattg ttatggttac attggccatg ttgtgtatgc tatttgtgat gtataaaata
     1621 ttatttgccg gatttggcga taaattgtat cccaaagaat tcaatgcatt ttcgttaatt
     1681 ccatttacaa taaacacatc gctcgggctg ctacagccat tgctgtcggt aatgatgaca
     1741 taataattac caagcagcga aggagtataa catgccgaag tgccaccaat agtaatgggt
     1801 gcattgttaa aataccattg ataggactgg gcttgtgagc aacatatttg tgagccattg
     1861 aaagtaacaa taggctttgg aggattggca acaaccgtga tgaagcttgt aattgttaat
     1921 gaatcctgtc caaaagaatt gtaagcaacc aacgttacag gataagtccc atacgaagga
     1981 aaataaattc ccgcagggtt ttgcatagta gaagtgtcgg gcgaagcacc gggaaaatac
     2041 cattgccaca tattgggatt gccagtgctg
//
LOCUS       DAORQA010000316         2057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11891_length_2057_cov_2.856643, whole genome shotgun sequence.
ACCESSION   DAORQA010000316 DAORQA010000000
VERSION     DAORQA010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11891_length_2057_cov_2.856643"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PK736_09450"
     CDS             complement(<1..794)
                     /locus_tag="PK736_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33651.1"
                     /translation="MRKFTLITLFVLIGIFSTKGQMQIIPLMVDSAISVSDIDIISQD
                     TIIFSSRSKIYRTTNGGMSWDSSETNLPNVQKLDFIDFDHGVVVHPIGMKRTYNGGLT
                     WDSIGFVPAYSYGDCHMIDTTHFIVTTDSGYIRHIYSNYYINDTLVDPLAIGYPVVIF
                     NFDFLDANEGFGGGVVNGFSAVIHTVDNGLTWDVRANSGFLYLYMHEISFFNPLIGAA
                     IEFGPGSFEKIVVTDDGGYTWQKSAYFDLTKVTSIDFNENGKGLAIT"
     gene            complement(791..1294)
                     /locus_tag="PK736_09455"
     CDS             complement(791..1294)
                     /locus_tag="PK736_09455"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33652.1"
                     /translation="MKIEKAQNNIVVANSCNYEMLNGSDIRMLAGNLIQMKPGFHAAN
                     GSIIHTKINSDCVPENVQRAADNISNGSKYINAQEKAKLTLSPNPAIEELNISLSNVN
                     AEKVTLIIFDNKGKQQKVLLENFSINNLSTAHFYFSINKLNLKRGAYVVKAQLGCEIL
                     TTKLVIQ"
     gene            complement(1347..>2057)
                     /locus_tag="PK736_09460"
     CDS             complement(1347..>2057)
                     /locus_tag="PK736_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33653.1"
                     /translation="IFRRGDYWLVKIDSVGNQIWDKDYGGVDAENKIGNIEQTLDGGF
                     IITGNSYSNQGGDKTEDNNAKIDAPWLVKTDSNGNIMWDKTIKTQPGWWHDELAFTIQ
                     NHDSCYTTVVSTQSDIGGYKTQPNWDTTGQTKDIWIIKHCDTTSNIGYATSHNHQNVL
                     MIYPNPNKGIFWIKTFIDNSISKSMFIYTITGKLIYKKENMIENIVQVDLSSEARGIY
                     FVKVVQQNNVLVGKVVVE"
BASE COUNT          623 a          400 c          289 g          745 t
ORIGIN      
        1 gtgattgcca aacctttacc attttcatta aaatcaatag acgttacttt agtcaaatcg
       61 aaataggcac tcttttgcca agtgtaacct ccatcatcag taacgacaat tttttcaaaa
      121 ctacctggcc caaattcaat ggcagcgcca ataagtggat tgaaaaatga aatttcgtgc
      181 atatacaaat ataaaaaacc actattggct cgtacatccc aagtaaggcc attgtccact
      241 gtatgtatca cagccgaaaa accattaacc acaccacctc caaaaccttc gtttgcatcc
      301 aaaaaatcga aattgaatat tacaactgga taaccaattg caagagggtc aactaatgtg
      361 tcgttaatgt aataattact gtaaatgtga cgaatataac cactatctgt cgtaacaata
      421 aagtgggtgg tgtcaatcat gtggcaatca ccatatgaat aagccggaac gaaacctatt
      481 gaatcccaag taaggcctcc attatatgtt cttttcattc ctattgggtg gactactaca
      541 ccatgatcaa aatctataaa atcaagtttc tgtacatttg gcaaattggt ttcacttgaa
      601 tcccaactca tgcctccatt agtagtacga taaattttgg acctcgaact aaagattata
      661 gtatcttgac taattatatc tatatccgaa accgatattg cactatcaac cataagcggt
      721 attatttgca tttggccttt tgtgctaaat atgcctatta acacaaacaa tgttattaat
      781 gtaaattttc tcattgtata actaatttgg ttgttaatat ttcacagcct aattgagctt
      841 ttacaacgta tgcacctcgt tttaaattta atttattaat tgaaaaatag aaatgtgcag
      901 tagaaaggtt gtttatcgaa aaattttcca acagaacttt ttgctgcttc cctttgttat
      961 cgaaaattat taaagttacc ttttcagcat ttacattgga taaggaaata tttaattcct
     1021 ctatggcagg gttaggactt agcgttaact ttgctttctc ttgtgcattt atatatttgc
     1081 ttccgttaga aatattatcc gcggctcttt gcacattttc aggaacacaa tcactattta
     1141 tttttgtgtg tataatgctg ccatttgctg catgaaagcc tggtttcatt tgtatcaagt
     1201 tgcccgcaag catacgtata tcgctaccgt taagcatttc atagttacaa ctatttgcaa
     1261 ccacaatatt attttgtgct ttttcaattt tcattttttg cggtaacatt tatttaatgc
     1321 tattgcaatc tcatttatat gttcttttat tctaccacca ccttacccac cagcacatta
     1381 ttttgttgaa ctacttttac aaaataaatt cctcttgctt cactacttaa atctacttgt
     1441 acaatattct caatcatgtt ttcttttttg taaattaatt ttcctgtgat ggtgtaaatg
     1501 aacatagatt ttgaaattga attatctata aatgttttta tccaaaaaat acctttgttt
     1561 gggttaggat aaatcatcaa tacattttga tgattatgcg aagtagcata tccaatatta
     1621 gaagtagtat cacaatgctt aatgatccaa atatcttttg tttggcccgt tgtatcccaa
     1681 ttcggctgtg ttttatagcc tcctatatca ctttgtgttg atacaactgt tgtataacaa
     1741 ctatcgtggt tttgtattgt aaatgccaac tcatcgtgcc accaacccgg ctgagttttt
     1801 atggttttgt cccacatgat atttccattc gaatcagttt ttaccaacca aggggcatct
     1861 atttttgcgt tgttgtcttc tgttttatca ccaccttggt ttgagtatga gttgcctgta
     1921 ataataaatc caccatccaa tgtttgttct atatttccta ttttattttc agcatctaca
     1981 ccaccataat ctttatccca tatttgatta ccaaccgaat ctatttttac caaccaataa
     2041 tcgcctctac gaaatat
//
LOCUS       DAORQA010000317         2048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11948_length_2048_cov_8.397893, whole genome shotgun sequence.
ACCESSION   DAORQA010000317 DAORQA010000000
VERSION     DAORQA010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11948_length_2048_cov_8.397893"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..>2048
                     /gene="uvrB"
                     /locus_tag="PK736_09465"
     CDS             89..>2048
                     /gene="uvrB"
                     /locus_tag="PK736_09465"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRC33654.1"
                     /translation="MEFKLTSEFKPTGDQPDAIAQLVQGLNKGDKAQTLLGVTGSGKT
                     FTVANVIAQVNRPTLILSHNKTLAAQLYGEFKTFFPQNAVEYFVSYYDYYQPEAYLPT
                     TNTYIEKDLAINSEIEKMRLSTTTALLSGRRDVIVVSSVSCIYGMGNPADFTESIIRI
                     HKGMRVSRNTFLHRLVTSLYSRTVIEFKHGTFRVKGDTIDIFLAYADYAIRVVFFGDE
                     IEEIESIDPISNGRIERLEHVAIFPGNIFVTNQERQLEAIFTIQADMMKQVEFFKDQG
                     RHLEAKRLEDRVTYDLEMIRELGYCSGIENYSRYFDGRVVGSRPFCLLDYFPEDYIMV
                     IDESHVTVSQIRAMYGGDRSRKVNLVDYGFRLPAAMDNRPLKFEEFDSLLHQVIYVSA
                     TPADYELEQSGGVIVEQLIRPTGLLDPVIEVRPSGNQVDDLLDEIELVVKNKERVLVT
                     TLTKRMAEELAKYLAKVGVKCRYIHSDVETLDRVEILRDLRLGVFDVLIGINLLREGL
                     DLPEVSLVAILDADKEGFLRSNRALVQTSGRAARNVNGKVIMYADRITESMRKTIEET
                     NRRREKQTKYNIENNITPTQIVRSTESVFGEQKKNANANGGKTYIEKDEVNVAADPLV
                     KFMTKEDLQKQLQKSKRNMEQAAKELDFM"
BASE COUNT          671 a          368 c          447 g          562 t
ORIGIN      
        1 gtgcagagta ttgcattaac aacaaattat tattcacaac caaaaaataa attacaaaac
       61 ttgcttcaac gtattgtaac tttgcaaaat ggaattcaaa ctcacatctg aatttaagcc
      121 cacaggcgat cagcccgatg ctattgcaca actggtgcaa ggattaaata aaggtgacaa
      181 agcgcaaaca cttttgggcg ttaccggatc gggtaaaacg ttcactgtag caaatgtaat
      241 tgcacaggta aatcgcccta cattaatttt gagtcacaac aaaactctag cagctcaatt
      301 gtatggtgaa ttcaaaacat tttttcctca aaatgctgtt gaatattttg tttcctatta
      361 tgattattat caacccgagg cctatctgcc tactacaaat acttacatcg aaaaagacct
      421 ggccatcaat agcgaaattg aaaaaatgcg actgagcacc actactgcct tgctatcggg
      481 caggcgcgat gttattgtgg tatcaagtgt atcgtgcatt tatggtatgg gaaatcctgc
      541 ggattttaca gagagtatta tacgtataca caaaggcatg cgcgtgtcgc gcaatacttt
      601 tttgcatcgt cttgttacca gtttatactc gcgcaccgtt atcgaattta agcatggtac
      661 atttagagtg aaaggcgaca caatcgatat ttttttagcc tatgccgatt atgcgatccg
      721 ggtggttttt tttggtgacg aaattgaaga aattgaaagt atcgatccta ttagcaatgg
      781 gcgcatagaa aggttagaac acgttgcaat atttcccgga aacatttttg taacaaacca
      841 agaacgtcaa ttggaagcca ttttcaccat tcaggctgac atgatgaagc aagtagaatt
      901 ttttaaagat caaggccgac atcttgaagc taagcggctt gaagaccgcg tgacttatga
      961 tttggaaatg atacgtgagt tgggatattg cagcggtatc gaaaattatt cgcgctattt
     1021 cgatggacgg gttgttggaa gccgaccttt ctgtttgctc gattattttc ccgaagatta
     1081 tataatggtg attgatgaaa gtcacgttac tgtttcgcaa atacgggcca tgtatggtgg
     1141 cgatagatcg cgcaaagtaa atttggtaga ttatgggttt cgactgcctg ctgccatgga
     1201 taaccgcccg ttgaaatttg aagagtttga tagtttatta catcaggtaa tttatgtgag
     1261 tgctaccccg gccgattacg aactcgaaca atcaggcgga gtaattgttg agcaacttat
     1321 acgtcctaca ggtttgttgg atcctgtaat tgaagtgcgc ccaagcggaa atcaagtgga
     1381 tgatttgctt gacgaaatag aacttgttgt aaaaaataaa gaacgcgttt tggtaacaac
     1441 gcttacaaaa cgcatggccg aagaactagc aaagtactta gccaaagtag gtgttaaatg
     1501 cagatacatt cacagtgatg ttgaaacatt ggatcgcgta gaaatattgc gagacctgcg
     1561 cttgggagtg ttcgatgtgt tgataggaat aaatttattg cgcgaaggat tagacttacc
     1621 cgaagtatcg ttagtcgcaa ttcttgatgc agacaaagaa ggatttttgc gcagcaatcg
     1681 cgcacttgta caaacatcag ggcgggcagc acgcaatgta aatggcaaag taattatgta
     1741 tgccgataga attaccgagt cgatgcgcaa gacaattgaa gaaacaaatc gcaggcgcga
     1801 aaaacaaaca aagtataata tcgaaaacaa tattacacca acacaaattg ttcgttccac
     1861 cgaatcagtt tttggagaac aaaagaaaaa tgcaaatgct aatggaggaa aaacctatat
     1921 tgaaaaagat gaagtaaatg ttgctgcaga tccattagtg aaatttatga ctaaagaaga
     1981 tttgcaaaag caattacaaa aatcgaaacg caatatggaa caggcagcca aagaactcga
     2041 ctttatgg
//
LOCUS       DAORQA010000318         2047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11958_length_2047_cov_6.245984, whole genome shotgun sequence.
ACCESSION   DAORQA010000318 DAORQA010000000
VERSION     DAORQA010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11958_length_2047_cov_6.245984"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PK736_09470"
     CDS             <1..1115
                     /locus_tag="PK736_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRC33655.1"
                     /translation="YKPNRTCYPGTEYFRMYIVVNVLQTFVLDAWGFPFWATVLIFIL
                     LILLYTYEGGVKTIIYTDTLQTTFMLAALVYCLYIIITQLNLSVDGLFTQMHASNYST
                     IFVTDKLSANYFLKNIFGGMAIAIAMTGLDQEMMQKNLSCKNIKEAQKNMMSFAAVLL
                     VVNILFLILGGALCLYATSQGITIDPTKTDNLFPTVALHYLGVVPAVIFLIGLISAAY
                     PSADGALTALTTAFCFDFLGIQKNKNLTEAQRKKTRYIVHGSFALVLLLTIVLFRVIN
                     NDAVIRNVFTAASLTYGPLLGLYAFGFFTKYTIRDNYAWIPCVISPILCWILRQNAAE
                     WLGGYKIGFELLIINGLITYLGLWLLANGKNELGDR"
     gene            complement(1275..>2047)
                     /locus_tag="PK736_09475"
     CDS             complement(1275..>2047)
                     /locus_tag="PK736_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33656.1"
                     /translation="HAILSIENTLQWIVMPADTESINNGMVEYGIANGLNCKLYFVLS
                     RSVNNHLQNIGVGQILENSFVTIAKFERAELLSFNLLKLEILFFDMESKKYRKPIITD
                     LSIELPGRDTVDLKLKGRFAFAKRKMIVNENIKELSTSELQHLVNTKTIVSKEPVVVN
                     KPAIAEIVVDLHTEAMEMKTHHLSNEAILEIQLDSFRRSLNDAIFKRYWRIIFIHGVG
                     EGVLKNKIRDELKHYKDLKVSDADFKEFGVGATAVILS"
BASE COUNT          618 a          376 c          354 g          699 t
ORIGIN      
        1 gctataaacc aaatcgaacg tgttatccgg gtactgaata ttttcgcatg tatatagttg
       61 taaatgtgct gcaaacattt gtgctcgatg catggggttt cccattttgg gcaacagtat
      121 taatttttat tttacttatt ctattatata cttacgaagg cggagtaaag accattattt
      181 acaccgacac attgcaaact acatttatgc tggcggcact tgtttactgt ttgtatatca
      241 ttataacgca attaaattta agtgttgatg ggttgtttac tcaaatgcat gcaagcaatt
      301 attcgacaat atttgttacg gataaattat cggcaaatta ttttctgaaa aatatttttg
      361 gtggcatggc catagcaatt gcaatgacag ggctggatca ggagatgatg caaaaaaatc
      421 ttagctgcaa aaacataaag gaagcacaaa aaaacatgat gtcgtttgca gcagtgttgt
      481 tggttgtaaa tattttattc ctcattcttg gtggtgcatt atgtttgtat gctacaagcc
      541 aaggcattac tattgaccct accaaaacag acaatttatt tcctacagtt gcattacatt
      601 atttaggagt ggtgccggcc gtaatttttt tgataggatt aatttcggct gcatacccaa
      661 gtgccgatgg tgcactcaca gcattgacaa cagctttttg ttttgacttt cttggcattc
      721 aaaaaaataa aaacctcaca gaagcgcagc gtaaaaaaac acgttacatc gtacacggct
      781 cgtttgcact tgttttgtta ttgaccattg tactgtttcg tgtaataaat aacgatgccg
      841 ttatacgtaa tgtatttaca gcagccagtt taacctacgg cccattgtta ggcttatatg
      901 cttttggctt tttcactaaa tataccatac gcgataacta tgcatggata ccatgtgtaa
      961 tctcccccat tttatgctgg atactgcgac aaaatgctgc cgaatggctt ggtggttaca
     1021 aaatcggatt tgaattgctg ataataaacg ggctaatcac ttatttgggt ttgtggttat
     1081 tagccaatgg caagaatgaa ttaggggaca gatgaacaca gacattttgg gataaaaaat
     1141 aattgccact aaatacacga aaggcactga ataatttctt acctctaaca atgaattatt
     1201 tagtattatt ccgtgatttt ggtggcaaaa aattattcaa ctttcattcg cgcatttgtg
     1261 gcaaaaaaaa ttcactacga taaaataaca gctgttgccc ctactccaaa ttctttgaaa
     1321 tctgcatccg atactttcaa atctttgtaa tgttttagct catcacgaat tttatttttc
     1381 agtactccct cccctactcc atgaatgaat ataatgcgcc agtatctttt aaaaattgca
     1441 tcattcaaac ttcgtctgaa actatccaat tgaatttcaa gtattgcctc gttgcttaga
     1501 tggtgtgttt tcatttccat tgcttcggtg tgcagatcaa ctactatttc ggcaatggca
     1561 ggtttattta ctacaacggg ttctttactt acgatagttt ttgtattcac taaatgttga
     1621 agttcgctgg tcgaaagttc ttttatattt tcgttcacaa tcattttcct ttttgcaaag
     1681 gcaaatcgtc ctttcaattt taaatcaact gtatcgcggc cgggcaactc aatagataaa
     1741 tcagtaataa ttggcttgcg atacttttta ctttccatat caaaaaacaa aatttccaat
     1801 ttcaaaagat tgaatgaaag caattcggct ctttcaaatt tggcaattgt tacaaaacta
     1861 ttttccaata tttgccctac accaatattt tgaagatgat tattaaccga gcgcgatagc
     1921 acaaaataaa gtttgcagtt caatccattt gcaattccat attctaccat accgttatta
     1981 atgctttcgg tatcggcagg cattactatc cactgcaaag tattttcgat ggataaaatt
     2041 gcatgtg
//
LOCUS       DAORQA010000319         2046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_11965_length_2046_cov_6.186339, whole genome shotgun sequence.
ACCESSION   DAORQA010000319 DAORQA010000000
VERSION     DAORQA010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11965_length_2046_cov_6.186339"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /gene="argS"
                     /locus_tag="PK736_09480"
     CDS             <1..938
                     /gene="argS"
                     /locus_tag="PK736_09480"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009410661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRC33657.1"
                     /translation="QNEWITSFETVKGFLNITIDATLYMHELNSDELLIEKNNVDVNP
                     VVVEYSSPNTNKPLHLGHIRNNLLGYSLAKIIEAAGNKVAKVNIVNDRGIHICKSMLA
                     WQLFGNGETPQSSGLKGDKLVGKYYVEFDKKYNEEAKQLLQQWNENKFDNSNNSIDVK
                     AKVEILKQNIVNATKPEAKEKAETELKDFAKANTTIFKQVQQMLRSWEAGDEATIALW
                     KTMNGWVYDGFAITYKNLGVDFEKIYYESETYLLGKKIVEEGLQKNIFYKKEDGSVWK
                     ASTINCFCVAMAHRFISRKIWERHTYVTMIFCLAE"
     gene            851..1684
                     /gene="argS"
                     /locus_tag="PK736_09485"
     CDS             851..1684
                     /gene="argS"
                     /locus_tag="PK736_09485"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRC33658.1"
                     /translation="MRGDGTSVYITQDLGTAYLRYDDFLFSRMIYVVGNEQDYHFKVL
                     KLILKKIGVEWWNRLQHFNYNMVDLPTGKMKSREGTVVDADDLIEETITNAQNMTREL
                     GKLEGVDDATAKQLFFDIGMGALKFYILKVDPEKRMLFNPEESIDINGCTGPFVQYTY
                     ARIQSLRRKALSSNSLKEYANMPWQQHMPDAKELELIKQVISYKETVMLAAEKLSPAI
                     VANYAYETAKIFNQFYHDHVAVDEQNIQTSAFRILLSTKTSNVLKHAFGLLGINMPER
                     M"
     gene            1734..>2046
                     /locus_tag="PK736_09490"
     CDS             1734..>2046
                     /locus_tag="PK736_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33659.1"
                     /translation="MKKILILAVSLIAFLFNAHAQNCQLQKQLFNQLQNSNSQKLMPT
                     AGDSIHVVHYDITIDSIINNSVKAIYANTKLKVKARVNNVNVISLSLLKLSVDSVKSN
                     ST"
BASE COUNT          747 a          353 c          395 g          551 t
ORIGIN      
        1 aacaaaatga atggattacc tctttcgaaa ccgtaaaagg atttctcaac atcactatcg
       61 atgctacact ttatatgcac gaattgaata gcgatgaatt acttatcgaa aaaaataatg
      121 ttgatgtaaa tcctgtcgtt gtagaatatt catcgcccaa taccaacaag cctttacacc
      181 ttgggcatat tcgcaataac cttttaggat atagccttgc aaaaattatt gaagcggccg
      241 gaaataaagt tgccaaagta aatattgtaa acgaccgcgg tattcatatc tgcaaatcga
      301 tgttggcatg gcaattattc ggcaatggcg aaacgccaca atcatcgggc ttgaaaggcg
      361 ataaactcgt agggaaatat tatgtggagt tcgataaaaa atacaacgaa gaggccaaac
      421 aacttttaca acaatggaac gaaaataaat ttgataacag caataattct atagatgtca
      481 aagccaaggt tgaaatactg aaacaaaata ttgttaacgc cacaaagccc gaagcaaaag
      541 aaaaagccga aactgagctg aaagattttg ccaaagccaa cactacaata tttaaacagg
      601 tgcaacaaat gttgcgttca tgggaagctg gtgacgaagc tacaattgca ttatggaaaa
      661 caatgaatgg atgggtgtat gatggatttg caattacata caaaaatctt ggtgtcgatt
      721 tcgaaaaaat atattacgaa agtgaaacat atttgcttgg aaaaaaaata gtagaagaag
      781 gtttgcaaaa aaacattttc tataaaaaag aagatggctc ggtgtggaag gcctcgacca
      841 taaattgctt ttgcgtggcg atggcacatc ggtttatatc acgcaagatt tgggaacggc
      901 atacttacgt tacgatgatt ttctgtttag ccgaatgatt tacgttgttg gtaacgaaca
      961 ggattatcat ttcaaagtgt tgaaactgat attgaaaaaa attggtgtgg aatggtggaa
     1021 tcgtttgcaa cattttaatt acaatatggt ggacctgcct acaggaaaaa tgaaatcgcg
     1081 cgaaggtacc gtagtggatg ccgatgacct tattgaagaa accatcacca atgcacaaaa
     1141 catgacacgc gaattgggga aactcgaagg agtagatgat gcaacggcaa aacaattatt
     1201 tttcgatatt ggtatgggag cattgaagtt ttacatacta aaagtggacc ccgaaaaacg
     1261 tatgctcttc aatcccgaag agtcgattga tattaacggt tgcaccggac catttgttca
     1321 atacacctat gcacgtatac aatcgctcag gcgaaaagcc ttatccagca atagtttgaa
     1381 ggaatatgca aacatgccat ggcaacaaca tatgcccgat gcaaaagaac ttgaattgat
     1441 aaagcaagta ataagttaca aagaaactgt tatgcttgct gccgaaaaat tgagccccgc
     1501 aatagttgca aactatgcat atgaaacggc aaaaatattc aatcagtttt atcatgatca
     1561 tgtagctgtt gatgagcaaa atattcaaac aagcgcattc agaattttac tctctaccaa
     1621 aacctcaaac gtgctgaaac atgctttcgg gttgctggga ataaatatgc cggagaggat
     1681 gtaaatgatt ttaatttcac atcgaaataa tttatatttt tgaaaaaaaa acaatgaaaa
     1741 aaatattaat acttgctgta agtctcatcg catttttatt taatgctcat gctcaaaatt
     1801 gtcaattgca aaagcaactg ttcaatcaat tgcaaaatag caattctcaa aaattgatgc
     1861 caaccgcagg cgattcaata catgtggtgc attatgatat aactatcgat tccattatca
     1921 acaactccgt gaaggcaatt tatgccaaca caaaattgaa agttaaagcc cgggttaata
     1981 acgtaaatgt catctcattg agtttactaa agctttcagt ggattctgta aagagtaatt
     2041 ccactt
//
LOCUS       DAORQA010000320         2039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_12002_length_2039_cov_7.307460, whole genome shotgun sequence.
ACCESSION   DAORQA010000320 DAORQA010000000
VERSION     DAORQA010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12002_length_2039_cov_7.307460"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1927)
                     /locus_tag="PK736_09495"
     CDS             complement(<1..1927)
                     /locus_tag="PK736_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017655601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRC33660.1"
                     /translation="MDFNYSSISYNHFKHDSGWYGIGNTCETARVKICVNVNAPITVT
                     ASSNAPICAGGNLNLFATNGANSYTWSGPNTFGSTLQNPTITGVTTAATGTYTVTVVD
                     VNGCTSTSQVGVVINATPDLQPLAPLANNCPTTTVDLTAITLVDNNGTTGTLSYFKDA
                     NLTISETNPSAVAVSGMYYIVKTTTSSPACTDVDSVQVTISPCLCTPPSMVPNTTHVS
                     CFGGNNGAIDIATSGGTASYTYLWSNGNTNEDLTGLTAGTYTVTVTDAQPCSTQLVVT
                     VNQPSAIVLSSTHTNASCFGLSDGSIDLTVSGGTSGYTYSWSSGASTEDVTGLAAGTY
                     TVVVTDFNLCTTTQSIVITSPPAIDGNVAVTNNQCYGNTSGVVNLTIYSDTIGEFARP
                     GLLISEVMANPVGTDSSYEFVELIATKTINFATTPYSVIFTNNGTATANGWKQGLSIS
                     YGFDISTGIVNKGDIVYVGGNQMVPTGTKIRSKSIGTAGDGGLGTATPANGVLGNGGA
                     NADGVAVFKGNIASVTSSTVPLDAIFYGAGPTPGSAVVSAGAAGYQLPVNDKYPGGKL
                     QSTSFVGYDATLAGNTLRATGTFNIATNSFSINRNWDSLFYVNNNLSQVSLIGSSTFL
                     WSNGATTEDIINLAAGSY"
BASE COUNT          581 a          435 c          423 g          600 t
ORIGIN      
        1 tataagaacc ggcagccaaa ttgattatat cttcggttgt tgcaccattc gaccataaga
       61 aagttgaact gccaatgaga gaaacctggc taaggttatt gttcacatag aataatgaat
      121 cccaatttct gttaatagaa aacgaattag ttgcaatatt aaatgttcct gttgcacgaa
      181 gtgtatttcc cgccaacgtt gcatcgtacc caacaaatga tgttgactgc aatttaccac
      241 cagggtattt atcgttaacc ggaagttgat atcctgcagc accggctgat actacagctg
      301 agccaggggt tggacctgct ccatagaaga tggcatcgag cggcactgtt gacgaggtta
      361 cacttgcaat gttgcctttg aatactgcaa caccatctgc attcgcgcca ccatttccca
      421 agacaccgtt agctggagtt gcagttccta agccaccatc accggcagta ccaatactct
      481 tagaacgtat ttttgttccg gtaggaacca tttgatttcc tcccacatat actatatcgc
      541 ccttgttcac aattcctgta ctaatatcaa aaccatacga aatcgacaat ccttgcttcc
      601 atccatttgc tgttgctgtt ccgttatttg taaaaattac tgaataaggt gttgtagcaa
      661 aattgatggt tttggtagca atcaattcaa caaactcata agaagaatcg gtgcctacag
      721 ggttggccat cacttccgaa atcaacaatc cagggcgagc aaattcccct attgtatcgg
      781 agtatatcgt aaggttcaca acaccgctgg tattaccata gcactggtta ttggttactg
      841 ccacattacc gtcaatggca ggaggagaag taattactat tgattgtgtc gttgtacaaa
      901 gattaaaatc ggtaaccacc acagtatatg ttcctgcggc caggcctgtt acatcttctg
      961 tagaagcacc gctactccat gaatacgtat aacctgatgt gccacccgat actgttaaat
     1021 ctattgagcc atcggataag ccgaagcacg atgcatttgt atgtgtgctc gaaagcacta
     1081 ttgctgacgg ttgattaact gtaacaacta attgagtaga acaaggttgt gcatcggtaa
     1141 ccgtaactgt atacgtaccg gctgtcaacc cggttaaatc ttcatttgta tttccattac
     1201 tccataaata agtatacgaa gcagtaccgc ccgatgttgc aatatcaata gcaccgttgt
     1261 tgccaccaaa acacgacaca tgagttgtat ttggaaccat agaaggtgga gtgcataaac
     1321 aaggtgatat ggtaacctga acagaatcaa catcggtaca ggcaggtgat gatgtagtag
     1381 tttttacaat ataatacata ccactcactg caactgcgga tggatttgtt tctgagatag
     1441 taagatttgc atctttaaaa tatgaaagtg tgcctgttgt tccgttatta tcaaccaatg
     1501 taattgctgt taagtcaaca gttgttgttg ggcaattatt tgcaagcggt gctaaaggct
     1561 gcaaatcggg tgtggcattt attactacac ctacctgaga agtgctggtg catccgttta
     1621 catcaacaac tgtaactgta tacgttcctg ttgctgcagt tgttactcct gttatcgttg
     1681 ggttttgaag tgttgaccca aaagtatttg gccccgacca ggtatagctg ttggctccgt
     1741 tagttgcaaa taaatttaaa ttgcctccag cacatatagg tgcatttgaa gaagccgtta
     1801 cggtgattgg ggcatttaca tttacacaaa tttttacacg ggcggtttcg caagtatttc
     1861 ctattccgta ccacccggag tcatgcttaa agtggttgta ggaaatactg ctgtagttaa
     1921 agtccaatta gataccaagg cattatatgc cagccattgt gccgctgacc tggcagtacc
     1981 atcattattc attttcgaag tacccgtagt tgcgttacta aatgtgatag cagtagtac
//
LOCUS       DAORQA010000321         2022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_12118_length_2022_cov_23.823081, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000321 DAORQA010000000
VERSION     DAORQA010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12118_length_2022_cov_23.823081"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PK736_09500"
     CDS             complement(<1..882)
                     /locus_tag="PK736_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33661.1"
                     /translation="MTKKLLLIFFFLSKSLLYAQTNINYFLSDTNCPSLPVPFYIDSN
                     QVLDVQWDFCPGDLDVTPLVTSYNNLNMAPRQVQLIKVNEKFYGFTVAISGVVSRINF
                     GKSMSNLPTQTDLQSNALPVTTLTGIDIEKRGNKFYCFIGDYNTSKIYLSILDSINQN
                     NIQFNELLVNNVGFYNVEIYAQYLFVANFAFNIITKIEFANGFENLPTSTTTINTNGL
                     PAGIKFTYDTYNQTYHAFCVKNGSKEITRFDFGNSLNNVPAETLVFTLSSEPYAVEVV
                     NDNNSWYVYAALLGENKI"
     gene            complement(888..>2022)
                     /locus_tag="PK736_09505"
     CDS             complement(888..>2022)
                     /locus_tag="PK736_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRC33662.1"
                     /translation="STFVKGYWSLGKNIGKANHPKQWDAQPCITPDGLTLYFTTYRDS
                     VNGTADIYYTQKNKNGEFGEAKPLIQVNTNGNEKSPFMHPDNRTFYFSSDSLPGMGGY
                     DIFMIKIDDSGQWGKPVNLGYPINTEADEVGFFVSTDGSRGYFASNKLKGGKGSWDIY
                     QFDLYEKIKPEKVLFLKGELKDENNDVISDAKLELKNLQTKETVNIDLDSATGKYSHV
                     VLFDEDYVMTVKKKEYVFESNYFSKTDSSLNEPKKLDIDMKRIKVGSSYQLNHVLFKT
                     KSTELDSSSQEMINNLAQFLQENKSIKISIEGHTDNVGNANDNMTLSNNRAKVVHDNL
                     IGLGISASRLSYKGFGASRPIKTNDTEEGRKENRRTQFVITEK"
BASE COUNT          583 a          370 c          336 g          733 t
ORIGIN      
        1 aattttattt tcgccaagca atgctgcata tacataccac gagttattat catttactac
       61 ttcaacagca taaggctcac tcgaaagtgt aaatacaagt gtttcggcag ggacattatt
      121 aaggctatta ccaaaatcaa atcttgttat ttctttcgac ccgtttttta cgcaaaaagc
      181 atgataggtt tgattataag tatcgtaagt aaattttata cctgcaggca gcccatttgt
      241 atttattgtt gttgtgcttg ttggtagatt ttcaaatccg ttagcgaatt caattttggt
      301 aattatgttg aatgcaaaat ttgctacaaa caggtattga gcataaattt ccacattata
      361 gaagccgaca ttatttacaa gtaattcatt aaattgtatg ttattttgat tgatagaatc
      421 taaaatggaa agatatattt tgcttgtatt ataatcacca atgaaacagt aaaatttgtt
      481 gcctcttttc tcaatatcta taccggtaag ggtagtaaca gggagcgcat tactttgtaa
      541 atctgtttga gttggtaagt tactcatgct cttcccaaaa ttgatacgcg atactacacc
      601 gcttatagca acagtaaatc cgtaaaattt ttcattaact tttattagtt gaacctgacg
      661 aggtgccata ttcaaattgt tgtaagaagt cactaaagga gttacatcca aatcgcccgg
      721 gcaaaaatcc cattgcacat caagcacctg gttgctatct atataaaacg gaacaggaag
      781 tgaagggcaa ttggtatcgg ataaaaaata attgatattt gtttgtgcgt ataacaaaga
      841 cttactcagg aagaagaaaa tcaaaagtag tttcttagtc atacaaatta tttttcggta
      901 attacaaatt gtgtacgtct gttttctttg cggccttctt cggtatcgtt tgttttaata
      961 ggtctgcttg caccaaatcc tttatatgat aacctcgaag ccgaaatgcc caagccaatt
     1021 aaattgtcgt gtactacttt tgctctgttg tttgatagtg tcatattatc atttgcattt
     1081 cctacattat cggtatggcc ttctatcgat atttttatac tcttattttc ttgcaaaaac
     1141 tgtgcgaggt tatttatcat ttcctgtgat gaagaatcga gttcagttga tttggtttta
     1201 aatagaacat gattcaactg ataagagctg ccaactttta ttcgcttcat atctatatcg
     1261 agttttttag gctcgtttag tgatgaatcg gttttgctaa aataatttga ctcgaaaaca
     1321 tattctttct tttttactgt cattacataa tcctcatcga ataataccac atgcgagtat
     1381 tttcctgtag cgctgtcgag gtctatattt actgtttctt ttgtttgtaa atttttcaat
     1441 tcgagttttg catcgcttat cacatcattg ttttcatcct tcaattctcc tttaagaaac
     1501 agtacttttt cgggttttat tttttcgtac aaatcaaatt gataaatatc ccaactgcct
     1561 ttaccgccct tcaatttgtt agaagcaaaa tagcctctgc ttccatcggt gcttacaaaa
     1621 aatcctactt catcggcctc ggtattaata ggatagccaa ggtttactgg cttgccccat
     1681 tgtccgctat catctatctt aatcataaaa atatcatagc ctcccatacc gggcaggcta
     1741 tcgctgctaa agtaaaatgt gcgattgtcg ggatgcataa acgggctttt ttcattgcca
     1801 tttgtattta cctgtatcaa aggtttggcc tcgccaaatt caccattttt gtttttttgt
     1861 gtataataaa tatccgctgt tccattcaca ctatcacggt aagtagtaaa gtataaagtc
     1921 aatccatcgg gcgtaatgca gggttgtgca tcccattgtt ttggatggtt ggccttgcca
     1981 atattttttc ctaacgacca atatcccttt acaaatgtac tt
//
LOCUS       DAORQA010000322         2020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_12138_length_2020_cov_3.333842, whole genome shotgun sequence.
ACCESSION   DAORQA010000322 DAORQA010000000
VERSION     DAORQA010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12138_length_2020_cov_3.333842"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..622)
                     /locus_tag="PK736_09510"
     CDS             complement(152..622)
                     /locus_tag="PK736_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33663.1"
                     /translation="MLKINSTKNLIAKAFLYFMFFAIAFSSCKKNSDEVSTTPSITIQ
                     SLTPGTIKQFSDTLTMIIAYEDGDGDLGENNSTAKNLFVPDKRINITTAYRISELAPQ
                     GSAINIKGTLKLYLSNIPVLNGNNSESYNYDVYVKDRAGNTSNVVTSSSVTVVK"
     gene            complement(653..1585)
                     /locus_tag="PK736_09515"
     CDS             complement(653..1585)
                     /locus_tag="PK736_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33664.1"
                     /translation="MKNNNQFIKIVLGMGIILIALILQQCKENDLPDNPYDGLISNVT
                     PPVVDTTSAFSIQGLHKNIFSPQCANPGCHDGTFEPDYRTIQSTYSTLVYHSVVKNNQ
                     GGTFKYRVVPYDTVNSWLHERLITGDSVLGQMPIYSTPLEQKEIDNVNKWIMNGAKDM
                     FGVPAVKAWLPNAPPVVAGYAAFIGNVQIDTNRINGINYNPFIVSSGTVMNVAFFVTD
                     DSTDVQNFTINKVKISTSPDDFSSAIVYNCIYLFIPGSGGVWYTPIDCSALPQNKVLY
                     MRYYVGDGIANNNVEFPTNASPNFYKTYFAFFIN"
     gene            complement(1602..>2020)
                     /locus_tag="PK736_09520"
     CDS             complement(1602..>2020)
                     /locus_tag="PK736_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33665.1"
                     /translation="LVVNMQDVDACHSDFTGYCNNLRKADYAVAHLWETIQNTPGMAN
                     DTIMIVAPEHGRNLVPNSIVDAYGRKALDHSSDATSREIFCLVLGPPNVVKQNNVINS
                     IKGESIDIVPTIANILGFDNSIAGQLQGSLLVDAFY"
BASE COUNT          612 a          365 c          357 g          686 t
ORIGIN      
        1 tcaaaatatt tttttatgtt taaaatcaaa gataaaaatt gtttttgatt caattttgat
       61 ttaaaaatgt ctgcaacaat ttatcaataa cgattaagaa aaaaagcaaa atcaaaaaac
      121 aactcattta aattgaacac actgcttatt actacttcac cacagttacg cttgagcttg
      181 taaccacatt acttgtattg ccggctctgt cttttacata cacatcataa ttatacgatt
      241 cgctgttgtt accattcagc acaggaatat ttgaaaggta aagttttaat gtgcctttaa
      301 tattgatagc agagccttga ggtgcaagct cacttatacg ataggcagtt gttatattta
      361 ttcgtttatc gggtacaaat aaattttttg cagtgctatt attttcacca aggtcgccat
      421 cgccatcttc gtatgcaata atcatagtga gggtatcgct aaattgtttt atggtgcctg
      481 gtgttaaact ttggatggta atgcttggcg tggtacttac ttcgtcacta ttttttttgc
      541 atgatgaaaa tgcaattgca aaaaacataa aatacaaaaa cgcttttgca atcaggtttt
      601 ttgtgctatt tattttcagc atagatttta aatttctatt tatcatcact atttaattta
      661 taaaaaatgc aaaataagtt ttgtaaaaat tgggcgatgc atttgttgga aattctacat
      721 tgttgtttgc aattccatcg ccaacgtaat agcgcatgta taacactttg ttttgtggta
      781 atgcactgca atcgatagga gtataccaca caccgcccga gccggggata aataaatata
      841 tacaattgta tactatggca ctcgaaaaat catcgggaga agtagagatt tttactttgt
      901 tgattgtgaa gttttgcaca tcggtactat catcggttac aaaaaatgcc acattcatta
      961 ccgtacctga cgatacaata aatggattat aatttattcc attaatgcgg ttagtatcta
     1021 tttgtacatt gcctataaat gcagcataac ctgcaacaac tggtggtgca ttgggcaacc
     1081 acgcttttac agccggcaca ccaaacatat ctttggcgcc attcattatc cacttattta
     1141 cattatcaat ttctttttgt tcgagtggtg tggagtatat tggcatttgc ccaagcacac
     1201 tgtcaccagt aattaaccgt tcgtgcagcc agctatttac cgtatcgtaa ggtactacgc
     1261 gatatttaaa agtgcctccc tgattatttt ttactactga atgatatacc agagtagaat
     1321 atgttgattg aatagttcga taatcgggtt cgaatgttcc atcatgacag ccgggattag
     1381 cacactgtgg cgaaaaaata tttttgtgta atccctgtat cgaaaacgca cttgttgtat
     1441 ccactacagg aggtgttaca ttgcttatca atccatcata cggattatcg ggcaggtcat
     1501 tctctttaca ttgttgtagt attaatgcaa tgagtattat gcccattccc aaaactattt
     1561 ttataaactg attattattt ttcattgtaa gaatttaatg attaataaaa tgcatcgacc
     1621 agcaaactgc cttgcaattg cccggcaata ctattatcga agcctaaaat atttgcaata
     1681 gttggcacta tgtcgatgct ttctccttta atggaattga ttacattatt ttgctttaca
     1741 acatttggtg ggccgagcac caaacaaaat atttcgcgcg atgtagcatc actggagtgg
     1801 tcgagtgctt tgcgaccata agcatcaact atagagttag gtacaaggtt tctaccatgt
     1861 tcgggtgcta ctatcataat agtatcgttg gccattccgg gagtattttg tatggtttcc
     1921 cataaatgtg caacagcata atcggccttg cgtaagttat tgcaatatcc ggtaaagtcg
     1981 gaatggcagg catctacatc ctgcatattt actacaagca
//
LOCUS       DAORQA010000323         2017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_12154_length_2017_cov_7.084608, whole genome shotgun sequence.
ACCESSION   DAORQA010000323 DAORQA010000000
VERSION     DAORQA010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12154_length_2017_cov_7.084608"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PK736_09525"
     CDS             complement(<1..378)
                     /locus_tag="PK736_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HRC33666.1"
                     /translation="MENYEIADKFKLLSQLMELHNENSFKIRSYQNASFKIDKMNDVK
                     LMECDLPTLEQMDGIGKSVASKIAELQQTGTINEIEKLQAVTPQGVLHMMKIKGIGPK
                     KVAVLWKELEVETLGELLYACNEN"
     gene            complement(455..1024)
                     /gene="purN"
                     /locus_tag="PK736_09530"
     CDS             complement(455..1024)
                     /gene="purN"
                     /locus_tag="PK736_09530"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRC33667.1"
                     /translation="MKRLILFASGSGTNAENIMKHFRHSNVVKVVAVMTDNPQAGVIE
                     RANKFDVPVLIFDKAQLNNPDHLLQKCKLLKPDLIVLAGFLKLIPRHFVNQFSKKIIN
                     IHPALLPKYGGKGMYGMHVHESVIANSENKSGITIHFVNEHFDEGEIIMQQSVDVSND
                     DAESLAKKIHHLEYKYLPLAIEEVLQQQN"
     gene            1155..>2017
                     /gene="rsgA"
                     /locus_tag="PK736_09535"
     CDS             1155..>2017
                     /gene="rsgA"
                     /locus_tag="PK736_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRC33668.1"
                     /translation="MTAKVLKSVGSLYTVCNDNNEIVTCKLKGKLRLKDFDSTNPIAV
                     GDNIEYEWDEVAGHGLITDVLPRHNYIVRKSVNLSKQKQILASNIDLAFLIVTPLYPR
                     TSTGFIDRFIATAEAYHITPVLVFNKADLFEGDGELLMEEYFKIYKPLDYKCIVASAI
                     NGKGIEEIKTLMHNKTSLFAGHSGVGKTTLINAIQPALNLRTGKISDQHAKGKHTTTF
                     AEMFLLDFGAYIIDTPGIRDLGTVDFVPQEISHYFVEMRERIHDCKFNNCLHENENNC
                     AIKQAVADGEI"
BASE COUNT          596 a          378 c          384 g          659 t
ORIGIN      
        1 gttttcgttg caggcataaa gcaattcgcc tagtgtttct acttcaagtt ctttccacaa
       61 cacagcaact ttttttgggc ctatgccttt tattttcatc atgtgcagca cgccctgcgg
      121 agttacggct tgcagctttt ctatttcgtt tatagtgccg gtttgttgca actctgcaat
      181 ttttgatgct acacttttgc caataccatc catttgttca agtgttggca agtcgcactc
      241 catcagtttt acgtcattca ttttatctat tttgaacgag gcattttgat acgagcgtat
      301 tttaaagcta ttttcgttgt gtagttccat cagttgcgaa agcaacttaa acttatcggc
      361 tatttcgtaa ttttccattt ttgtataata ggtggatagg agaaaaaact cggtccgaag
      421 aaaatacttt tgggattgca ataaaaattt ttggtcaatt ttgttgttgc aacacttctt
      481 caattgcaag cggcagatat ttatattcaa ggtggtgaat tttttttgcc agcgattcgg
      541 catcatcatt cgatacatcc acactttgtt gcataataat ttcgccttcg tcaaaatgtt
      601 catttacaaa gtgaattgta atacctgatt tgttctcgct atttgcaata accgactcat
      661 gcacgtgcat accgtacatt cctttgcctc catactttgg caaaagtgcc ggatgaatat
      721 tgataatttt ttttgaaaat tgatttacaa aatgccgcgg tatgagtttc aaaaatcctg
      781 ctagtacaat caaatcgggt ttcaacaatt tgcacttttg caataaatga tccggattat
      841 taagctgtgc tttatcgaaa attaataccg gtacatcaaa tttatttgca cgttcaatta
      901 caccggcctg cggattatca gtcattaccg caactacttt taccacattg ctgtgtcgaa
      961 agtgcttcat aatattttct gcattggttc cacttcccga ggcaaaaaga ataagacgtt
     1021 tcattgttgt gtgcaattaa attaaaggca aagttatatt aaggtattac atgctgctat
     1081 ttttttgtag tgggcattat ttttttttaa tacaaaatga agtgagataa aaattttcta
     1141 accatattcg ccacatgact gcaaaggttt taaaatcggt tggtagcttg tatacggtat
     1201 gcaacgataa taacgagata gttacatgca aactaaaggg gaagttgcgc ctcaaagatt
     1261 ttgacagtac taatccaata gcggtgggcg ataatattga gtacgaatgg gacgaggtgg
     1321 ccgggcatgg attgataacg gatgtgctgc caaggcataa ttatattgtt cgcaagtcgg
     1381 taaatttatc gaagcaaaag caaattctgg cttcaaatat cgacctcgct tttcttattg
     1441 ttactcctct ttacccgcgt acctcaacag gatttataga taggtttatt gccactgccg
     1501 aggcatacca cattacacct gttttggttt ttaataaagc cgatttgttt gaaggcgatg
     1561 gcgaattatt gatggaagaa tattttaaaa tttacaaacc actcgattac aaatgcatag
     1621 ttgcaagtgc tatcaatggc aagggtatcg aagaaataaa aacactgatg cacaataaaa
     1681 catccttgtt tgccgggcat agtggcgtgg gaaaaacaac acttatcaat gcaattcaac
     1741 cggcattaaa tttacgtaca ggaaaaattt cggaccaaca tgcaaagggc aaacacacta
     1801 ccacatttgc cgaaatgttt ttgctcgatt ttggtgctta cattatcgat acacccggta
     1861 ttcgcgattt gggtacagta gattttgtgc ctcaagaaat atcacattat tttgtggaaa
     1921 tgcgcgagcg aatacacgat tgcaaattca ataattgcct gcacgaaaac gaaaataatt
     1981 gcgcaataaa acaagccgtg gccgatggcg aaattca
//
LOCUS       DAORQA010000324         2001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_12259_length_2001_cov_18.597122, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000324 DAORQA010000000
VERSION     DAORQA010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12259_length_2001_cov_18.597122"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1529)
                     /locus_tag="PK736_09540"
     CDS             complement(237..1529)
                     /locus_tag="PK736_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HRC33669.1"
                     /translation="MRYASIPPEVFIDNRRRFTAMLKPQSVAVFNSNDILPTNADGSM
                     AFRQNNDLFYLSGIDQEESILCIFPDCVDEKHREILFLKETSETIAIWEGEKHSKKSA
                     YEASGIRTVYWLQDFHRILKALVCESENVYLNSNEHLRASIETETRDMRFVKWMKDNY
                     PLHNYQRSAPVMHQLRSVKSNMELELLQTAIDITEKGLRRILKFIKPDVWEHEIEAEF
                     AHEFLRNRSRGFAYTPIIASGYSACVLHYIENNKQCKAGDVILLDVGAEYANYNADMT
                     RCLPVSGKFTPRQKDVYNAVHRVMEAAKKMLRPGTMMADYHREVGKLMTEELITLKLI
                     TREDVKKENADWPAYKKYFMHGTSHFLGLDVHDVGLWHKPIQPGMVFTVEPGIYIREE
                     NLGIRLENNVMVTMGEPLDMFKNIPVQAEEIEQLMNEA"
     gene            complement(1710..1895)
                     /locus_tag="PK736_09545"
     CDS             complement(1710..1895)
                     /locus_tag="PK736_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33670.1"
                     /translation="MFEYTKEILQKVSFDKELFGKELKKAVKWLKYDERKLLMMWCIA
                     TFGNQYIDIIHQVFKVK"
BASE COUNT          600 a          437 c          353 g          611 t
ORIGIN      
        1 gattgtttcg cagttcgaga atcccttcga ctgcgctcag ggtgacaaga ctcacagtga
       61 caaatcgcaa taacgttaat ggctaatgac cccttcgact tcgctcagtg tgacaatgac
      121 caatgagtgg cagagatgtt cctttcctca gcatgacaaa gtcaacaacc aaacaactca
      181 ataatcaata actatttcac aatgaccaat gaccatcctc aaatgaccaa tgaccattac
      241 gcttcattca tcaactgctc tatctcctca gcctgcacag gaatattttt aaacatatcg
      301 agtggttcgc ccatggttac catcacattg ttttcgaggc gaattccaag attttcttcg
      361 cgaatataaa tgccgggttc aactgtaaaa accataccgg gttgaatggg tttatgccac
      421 agtcctacat cgtgaacatc gagaccaaga aagtgcgaag tgccgtgcat aaaatatttt
      481 ttgtatgccg gccaatcagc gttttccttc ttcacatctt cgcgcgttat cagttttagt
      541 gttattaatt cttcggtcat taatttacct acttcacgat gatagtcggc catcatagtg
      601 ccgggacgca acattttttt tgctgcttcc attacacgat gcacagcatt atacacatct
      661 ttttgtcgtg gtgtaaattt gccacttaca ggtagacagc gtgtcatgtc ggcattgtag
      721 tttgcatact ctgcacctac atcgagcaaa ataacatcgc ccgctttaca ctgcttatta
      781 ttttcgatgt aatgcaatac acatgcactg tagcccgaag caataatagg agtgtaagca
      841 aaaccccgcg agcggttacg taaaaattcg tgggcaaact cggcttctat ttcatgctcc
      901 cacacatcgg gttttataaa ttttaaaatt ctgcgtaatc ccttttcggt tatatcaatg
      961 gcagtttgca aaagctccaa ttccatatta gattttaccg aacgcaattg atgcattaca
     1021 ggagcgctgc gctgatagtt gtgcaaagga taattatctt tcatccattt tacaaagcgc
     1081 atatcgcgcg tttcggtttc gatagatgca cgcaaatgtt cgttgctatt gaggtacaca
     1141 ttttcacttt cgcacaccaa agcctttagt attcggtgaa aatcctgcaa ccaatataca
     1201 gtgcgaatac ccgaagcttc gtaagcactt ttcttgctgt gtttttcgcc ttcccatatg
     1261 gctatggttt cgctggtttc tttcaggaat aaaatttcgc ggtgcttttc gtcaacacaa
     1321 tcgggaaaaa tacaaaggat gctttcttct tgatcgatac ccgagaggta aaacaaatcg
     1381 ttattttggc gaaaagccat acttccatcg gcattggtag gtaaaatatc gttactgttg
     1441 aaaaccgcca cagattgtgg tttcagcatg gcagtaaatc gtctgcggtt atctataaat
     1501 acttccggtg gaatgcttgc gtagcgcata gtgttttgtt ttttattgtt aaagttttca
     1561 aatgtatgga ttaattgaaa aacaaaacca acccatcaat aaaaatcgat ggcttcaatt
     1621 cgcactaaaa caaaacaagt tggataatat taaaaccggg taaaaactgt tgtaagaaat
     1681 aatgtatatc cccccccaaa aaaaagaaat cacttcacct taaaaacctg atgaataata
     1741 tcgatgtatt gattgccgaa tgtggctatg caccacatca ttagcaattt gcgctcgtca
     1801 tattttagcc actttacggc tttcttcaat tctttaccaa acagttcctt atcgaaactt
     1861 accttttgca atatttcttt tgtatactca aacataaaat gaacgattaa atgtgaaacc
     1921 aaaaataact attagtacgc cctaaaaaaa aatttattgt gccgaaacgg gataatttcc
     1981 taacaaacga ttctttataa a
//
LOCUS       DAORQA010000325         2001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_12264_length_2001_cov_5.136177, whole genome shotgun sequence.
ACCESSION   DAORQA010000325 DAORQA010000000
VERSION     DAORQA010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12264_length_2001_cov_5.136177"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /gene="mnmA"
                     /locus_tag="PK736_09550"
     CDS             complement(<1..836)
                     /gene="mnmA"
                     /locus_tag="PK736_09550"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRC33671.1"
                     /translation="MSKNGRILVAMSGGIDSSVTAMLLHEQGYEVIGITMKTWDYAGA
                     GTSKKETGCCSLDSINDARSIAVNKGFPHYILDIRSEFGDFVIDNFVDEYIAGRTPNP
                     CVLCNTYIKWDALVRRADMLNCEFIATGHYANIRNENGRYVISKGIDENKDQSYVLWG
                     LNQESMARTKFPVGQFRKQEIRDMAFEMGFYELSKKSESYEICFVPDNDYRSFLKHKK
                     PELIDQLEGGSFVATDGKILGKHRGYPFYTIGQRKGLEVAFGEPMYVTNINPKNNTVT
                     LG"
     gene            complement(999..1913)
                     /locus_tag="PK736_09555"
     CDS             complement(999..1913)
                     /locus_tag="PK736_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRC33672.1"
                     /translation="MQHKSLIDLKQNDRFNFELKALLSDEIEESGINLFMACEVTQHP
                     YINGNKFYKLKYNIEEFFRTGAKTVLTFGGAYSNHIAATAAMCHEQHIESIGIIRGQE
                     LNAESNKTLIMAANHGMQLIFVNRGEYSRRNDVTYNNELKNRFENIYIIPEGGANVMG
                     AKGSSEMVTMLEQKFDTYIIPVGTGTTLAGIANELLAQQKALGIMVHNGQKGVEDFLK
                     QLQFQMMKNWGDKIIFSNDYTFGGYGKTTAELEKFCHAFYLKYAIKIEPVYTGKMMFA
                     VFDLIKKGFFKRGQKIVCFHTGGLQYLQ"
BASE COUNT          601 a          418 c          323 g          659 t
ORIGIN      
        1 aagcccagtg taactgtatt atttttagga tttatgttgg taacatacat aggctcgcca
       61 aaagccactt ccaaaccttt gcgttgaccg attgtgtaaa atggataacc tcgatgctta
      121 ccaagtattt tgccatcggt ggctacaaaa ctacctcctt cgagttggtc aataagttca
      181 ggttttttgt gctttaaaaa agagcgatag tcgttgtcgg gcacaaaaca aatctcataa
      241 ctttcgcttt ttttcgatag ttcataaaag cccatttcga aggccatatc acgaatttct
      301 tgtttgcgaa attgcccaac cggaaattta gtgcgtgcca tactttcctg attcaatccc
      361 cacaaaacgt agctttggtc cttgttttcg tctattcctt ttgaaatcac atagcgccca
      421 ttttcgttac gaatattggc ataatggcct gtggctataa actcacaatt aagcatatcg
      481 gccctgcgta ccagtgcatc ccatttgatg taagtattgc aaagtacgca agggttgggc
      541 gttcgtcctg ctatatactc atccacaaag ttatctatta caaagtcgcc aaactcgctg
      601 cgtatatcta agatatagtg tggaaaacct ttatttacag caatactacg cgcatcattt
      661 atcgagtcga gcgagcaaca gccggtttcc tttttcgaag tacctgcacc ggcatagtcc
      721 catgttttca tggtaatccc tataacttcg tacccttgct cgtgcaataa cattgcagta
      781 acactgctgt cgatgccgcc actcattgca accaatatgc gcccgttttt actcatgacg
      841 caaaaatagt gttttccctt gtaatagcat taaatgtttg tgatgtctct caaaaagcaa
      901 atctccttga gccgtaaaaa taagatgtaa aaaaagaata attgattaaa agaatattga
      961 acaaaaaacc ctgtcaaatt cctttttgca agactccttc attgcaaata ctgcaatcct
     1021 ccagtatgaa aacaaacgat tttttggcca cgtttaaaga atcccttttt tatcaaatca
     1081 aaaacagcaa acatcatttt accggtgtaa acaggctcta tctttatagc atatttaagg
     1141 taaaacgcat ggcaaaattt ttcaagttca gcagtggttt ttccataacc tccaaaagta
     1201 taatcattag aaaatataat tttatcaccc cagtttttca tcatttgaaa ttgcaattgt
     1261 ttcaaaaaat cttccactcc tttttggcca ttatgaacca ttatgcctaa tgctttttgc
     1321 tgggctaaca actcattagc aattccggca agggtggttc cggtacctac gggaataata
     1381 taagtgtcaa atttttgttc aagcatggta accatttcac tgcttccctt tgcacccata
     1441 acattggctc caccttcggg aattatataa atattttcga acctgttttt caattcattg
     1501 ttataggtga catcatttcg ccttgaatat tctcccctgt taacaaaaat taattgcatg
     1561 ccatggtttg cagccattat caatgttttg ttcgattcag catttaactc ctggcctctg
     1621 attataccta tagattctat atgttgctcg tggcacatag cagcagttgc agcaatgtgg
     1681 tttgaataag caccaccaaa agtcaaaaca gtttttgctc ctgttcgaaa aaattcttcg
     1741 atattatatt taagtttata aaatttattg ccattaatat aaggatgttg tgttacttcg
     1801 cacgccatga ataaatttat tccgctttcc tcaatttcat cagatagcag tgctttaagt
     1861 tcaaaattaa atctatcgtt ttgcttcaaa tctattaaac ttttgtgctg caaattactc
     1921 taaaaaaaat cgaatgcatg agttggttag aaaaatacga taaagaagat tactatctta
     1981 ccgaagaagg atatcttgta t
//
LOCUS       DAORQA010000326         1990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_12333_length_1990_cov_6.002584, whole genome shotgun sequence.
ACCESSION   DAORQA010000326 DAORQA010000000
VERSION     DAORQA010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12333_length_1990_cov_6.002584"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1990)
                     /locus_tag="PK736_09560"
     CDS             complement(<1..>1990)
                     /locus_tag="PK736_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33673.1"
                     /translation="AVFRTGVSMTSGSGTFSFCAGDSLTLSAAAGFNAYLWSNGKSTQ
                     SIIVKTGGTYTVTVTGTGGCQTSTQRAVTVNPLPTVTFTNPYAATANKVCYTDAGVVL
                     NSYGTPAGGGFTGTGVYYNGFGTYSFVPSTTYGARTLVYTYTNTNGCTKTASTVITVD
                     SNTIVNAGGDQIKCGGSNAVQLAATKGGKSTATTWSTSGTGTFNNVNALNAIYTLSSA
                     DINAGSVTLTITTADPVGTCGPVNDNMVITIYQNAVANAGSNVPVCVPNVVGLNGSIT
                     GYPLATPLWSSTGSGTFANATALNTTYTASTADINAGKVFLILRPIDPNGQCSFTGDT
                     LEVWFDKYTVTAGANVVACGTTTVAITASKSTAVTSTVWTTNGTGTFGNANALNTNYT
                     LSNADKALGGVTLTITTNDPNGPCGAKSSSLFATYKAATADAGPAIICGDSIWQLNGT
                     YSAVPGTFCNASWSSLGTGTFNNANIFNPVYTASTADKTAGKVKLVLSLIDQAGQCSN
                     FIKDTIEVWFNPHYTVNAGPDSAFICGDKVLTLNGSKQKATSTVWSTSGTGTFANANA
                     LSTTYTFSNADTAANIITLYLTSNDPTGPCFVVVDSMKVVIKDVPKATAGTNSPVCIT
                     ANLNLTASGGSTYVWSGPNAYSSTLQNIVRANASLQMAG"
BASE COUNT          548 a          427 c          415 g          600 t
ORIGIN      
        1 cctgccattt gtaaacttgc attggcacga acaatatttt gcaaagtgga tgaatatgca
       61 tttggcccac tccaaacata agtactaccg ccactggcag ttaaatttaa attcgctgtt
      121 atgcacacag gagagttggt gccagctgta gctttaggca catctttgat caccactttc
      181 atactatcaa ctacaacaaa gcaaggtcct gtggggtcgt tcgatgtaag atatagtgta
      241 atgatattag ctgctgtatc ggcattggaa aaagtgtaag tagttgataa ggcgttggca
      301 ttcgcaaaag tgccggtacc gcttgtactc caaacagtgc ttgttgcttt ttgctttgag
      361 ccattcaatg ttaatacttt atcgccacaa ataaacgctg aatcgggccc ggcatttacc
      421 gtataatgag ggttaaacca aacttcaatt gtatctttta taaagttact gcattgcccg
      481 gcttgatcaa tcaagcttaa aaccaacttc actttgcctg cagttttatc ggcagtggaa
      541 gcagtataaa ccgggttgaa aatatttgcg ttattaaatg taccggttcc taatgaagac
      601 catgatgcat tacaaaaagt gccgggtact gcagagtatg ttccattcaa ttgccagata
      661 gaatcgccac aaataattgc cgggcctgca tcggcagtgg ctgctttgta tgttgcaaat
      721 aaactgctgc tttttgcacc acatggaccg ttgggatcgt ttgttgttat tgttaatgtt
      781 actccgccaa gggctttgtc agcatttgat aatgtgtaat ttgtattcaa ggcatttgca
      841 ttgccgaatg ttcccgttcc atttgttgtc cacactgttg aagttaccgc agtagatttt
      901 gaagcggtaa tcgctacagt agtagttcca catgctacca cattagcacc tgcagtaaca
      961 gtatatttat cgaaccaaac ttcgagtgta tcgccagtga atgaacattg cccatttgga
     1021 tctatcggcc tgagaattaa aaatacttta cctgcattta tatcggctgt tgatgctgta
     1081 taagttgtgt tgagcgcagt agcatttgca aatgtgccgc tgcctgttga tgaccaaagt
     1141 ggtgtagcaa gcggataacc tgtgatagag ccatttaatc caaccacatt tggcacacac
     1201 accggaacat ttgagcccgc attggccacg gcattttgat aaattgtaat aaccatattg
     1261 tcgttaacgg gtccacacgt accaacagga tccgctgttg taattgtcaa tgttacactg
     1321 cccgcattta tatcggcaga ggataaagta taaatggcat tgagtgcatt tacattattg
     1381 aaagtgcctg taccactagt actccatgta gttgctgttg atttaccacc ttttgtagcg
     1441 gctaattgca cagcgttgct tccaccacat tttatctgat cgccaccggc atttacgata
     1501 gtattactat ccacagtaat aacagtactt gctgtttttg tacaaccatt ggtattggtg
     1561 taagtgtata ccaaagtacg cgcaccgtat gtagtagaag gaacaaaact atatgtgcca
     1621 aatccattgt agtacacgcc agtgcctgta aatccgccac cggcaggcgt tccataactg
     1681 ttcagaacta ctcccgcatc ggtatagcaa actttatttg cagttgcagc ataaggattt
     1741 gtaaatgtta ccgttggcaa tggatttact gttaccgctc gttgagttga agtttgacat
     1801 cctccggttc cggtaacagt aacagtatac gtaccaccgg tttttacaat tattgattgg
     1861 gttgattttc cattgctcca taaataagca ttgaaacctg cagcagcact aagtgttaaa
     1921 ctatcaccgg cacaaaatga aaacgtgcca ctgcccgaag tcatgctcac tcctgtgcga
     1981 aatactgctt
//
LOCUS       DAORQA010000327         1974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_12435_length_1974_cov_8.672225, whole genome shotgun sequence.
ACCESSION   DAORQA010000327 DAORQA010000000
VERSION     DAORQA010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12435_length_1974_cov_8.672225"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1956)
                     /locus_tag="PK736_09565"
     CDS             complement(<1..1956)
                     /locus_tag="PK736_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33674.1"
                     /translation="MNSIYFINSSTAYIGGNNKMAFTNNDFASVTAINFLVDIQGNVV
                     PYFPNVINDLHFTSVNDGFCTGIFNLQNEYDVFKTDNGAANWTFQLNDNNGGLPRYLS
                     AFDFKGSIAVSVGSAGRMYRSSDGGDNWTGVNSNTFEDLLDVTFLQGNNVISCGDGVV
                     SRSSDNGLTWAADTSFNNDFIYALSKAPNSNTVFMNSSSIVYKSTNGGTTFNSYTFPF
                     SNMQHIFAINTDTVLVGTGAGVYISYDGALHWQQFATTQNFIVNKVAYNAPYMYALCD
                     SSTLLRADINTTPAEPFASFDYIEDNLCGVTHVTCTNNSDPGYTMAWYLNGAFVSSSY
                     NYNIDYTVTTPNQEIKLIVTSPSGTDTAIVKNSIYVKKLAVAIAQPDEYNCYGQEMIL
                     SGKDTLGQYMAWLPQSKLNLLYFGLNADSVLTKPILQQQDTIIFQTKNNDNCIALDTV
                     FIFTTPPAFEPFKKVVPGLKDFCSNAPCNFISGLDFINNQEGFGFANTTEFLKTTDGG
                     ATYSKLFLQPLFSQMNGPNFSSCDIDFIDQNIGYIGWGPLKTTDGGTTWNKIQNFLSI
                     TDGQLSFLNADTGIFSKGAGELYVTDDGGVSYSTIYSSVANGYMLIKDIFMYDDNHLF
                     IAGAKSAFGYNTEFKKSTDGGATWIALN"
BASE COUNT          637 a          378 c          337 g          622 t
ORIGIN      
        1 atttagtgct atccatgttg caccgccatc agttgatttt ttaaattcgg tattatatcc
       61 aaaagctgat tttgctcccg caataaataa atgattatca tcatacataa aaatatcttt
      121 aataagcatg tagccgtttg ccacactcga ataaattgtg gagtaactta cgccaccatc
      181 atcagtaaca taaagttcac cagcgccctt gctaaaaata cctgtatcgg cattcaaaaa
      241 actcaattgc ccatcggtga tgcttaaaaa attttgaatt ttattccatg tggtgccgcc
      301 atctgtagtt tttagtggtc cccagcctat gtacccaata ttttgatcaa taaaatctat
      361 atcgcacgat gagaaatttg gcccgttcat ttgtgaaaac aaaggctgca aaaaaagttt
      421 tgaataagta gcgccaccat cagtggtttt taaaaattct gttgtgttgg caaaaccaaa
      481 accttcctgg ttatttataa aatccaatcc cgaaataaaa ttacatggcg cattgctgca
      541 gaaatctttt aagcccggta ctactttttt gaaaggttcg aaagccggtg gagtagtaaa
      601 aataaaaaca gtatcgagcg caatgcagtt atcattgttt tttgtttgaa agattatggt
      661 atcctgttgt tgcaatattg gcttcgtaag aacgctatct gcatttaatc caaaatacaa
      721 aagattcaat ttgctttgcg gtaaccatgc catgtattgc ccaagggtat ccttgccaga
      781 taaaatcatt tcttgtccgt agcaattgta ttcatcgggc tgtgcaattg ctacagctaa
      841 ttttttcaca taaatactat ttttaactat agctgtatcc gtacccgatg gtgatgttac
      901 tataagttta atttcctgat tgggtgtagt aactgtataa tcaatattgt aattgtagct
      961 gctgcttaca aaagcaccat ttaggtacca ggccattgta taaccagggt ccgaattatt
     1021 tgtacaagta acatgcgtta cgccacacaa attatcttca atataatcga acgatgcaaa
     1081 aggctcagca ggcgtggtat ttatatcggc acgcaataaa gtactgctat cgcataatgc
     1141 atacatgtat ggagcattgt atgcaacttt atttacaata aaattttgtg ttgtggcgaa
     1201 ttgttgccaa tgcaatgcac catcatacga aatgtaaacg cctgctcctg taccaactaa
     1261 aacagtatca gtatttattg caaaaatatg ttgcatgttc gaaaatggaa atgtgtatga
     1321 attaaaagta gtaccaccat tagttgattt ataaacaatg gaagatgaat tcataaaaac
     1381 agtattcgaa ttgggtgcct tactcaatgc ataaataaaa tcgttgttga atgatgtgtc
     1441 agcagcccat gttaagccat tgtcgctcga acgcgataca acaccatcgc cacatgaaat
     1501 tacgttattg ccttgcaaaa atgttacatc aagcaaatct tcaaaagtgt tgctgtttac
     1561 acctgtccag ttatcgccac cgtcactgct acgatacatc ctacctgccg aaccaacact
     1621 tacggcaata gatcctttaa aatcgaaagc acttaaataa cgtggcaagc ctccattgtt
     1681 atcattcaat tgaaaagtcc aatttgcggc accgttgtct gttttaaaaa catcatattc
     1741 attttgcaaa ttgaaaatgc ctgtacaaaa tccatcgttt accgaagtga agtgcaaatc
     1801 gttaattaca tttgggaaat acggtactac atttccctgt atatcaacca aaaaatttat
     1861 ggcagttacg cttgcaaaat cattattggt gaaagccatt ttattattgc ctccaatata
     1921 tgcagtgctg ctatttataa aataaataga attcaaatat tgattgttga tggg
//
LOCUS       DAORQA010000328         1968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_12481_length_1968_cov_10.239937, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000328 DAORQA010000000
VERSION     DAORQA010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12481_length_1968_cov_10.239937"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1968)
                     /locus_tag="PK736_09570"
     CDS             complement(<1..>1968)
                     /locus_tag="PK736_09570"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRC33675.1"
                     /translation="IYIQKADATEAAKQLTKNADTYKYGFIWSPDSKKILWNDQQKRL
                     NCLDVVSGNMMEIAKSVTGEINDFTWSSDSKWVSYSDSKVGQKNILYLYEMATAKITP
                     ITTQWYDSYSPSFSSDGKYLFFISDRDFNPTYSNTEWNHSYSDMSKIYFVTLAANTTS
                     PFAPVNDAVAITNSEESKPATDDKTKDAKKKDEPKVAASKDVKIDLDGIQNRIGVLPI
                     SASGYFGLSIIDNSVYYMKRGNGDEATALKLFDLKDKKETDLGEIDGYEIGNNNKKML
                     VRKTKSYYVIDLPKGKLDLKESINTSDVKVLVDRKAEWKQIFDESWRQMRYFFYDSNM
                     HGVDWPAMKAKYEVLLPYVNHRADLSYIIGEMIGELSIGHAYVGGGDLPKAERIQLGL
                     LGADLVREANGYYRIAKILKGENWTGNLRCPLTDIGVNAKEGDYIVAVNGMPTNTLND
                     INVAFVNTVGKQVELQINSQASATGARKVLVTPISTQAPLEYYTWVQKNIDRVSAATD
                     GKVGYLHIPNMGVEGLNEFAKYFYPQLNKEALIIDDRGNGGGNVSPMIIERLNRELAM
                     INVARNTAPYTNPFQMVNGPKVVLINEYSASDGDLFPYRFKKYNMGKVIGKRSWGGVV
                     GIRGTMFFVDGGYLNRPEFGPYDIEGKQWII"
BASE COUNT          502 a          455 c          345 g          666 t
ORIGIN      
        1 ctataatcca ctgtttgcct tcgatgtcgt aaggcccgaa ttcaggacga ttgaggtaac
       61 caccatcaac aaaaaacatg gtgccacgaa ttccaactac accgccccaa ctgcgtttgc
      121 caattacttt tcccatatta tattttttaa aacgataagg aaacaaatcg ccatcgcttg
      181 cactgtattc atttatcaaa actactttgg ggccattcac catttgaaat ggattggtgt
      241 acggtgcggt gtttcgtgct acatttatca tggccaattc gcggttcaat cgttcgataa
      301 tcattggcga tacattgcca ccaccattgc ctcggtcgtc aattatcagt gcttctttgt
      361 ttaattgcgg atagaaatat tttgcaaatt cgttaaggcc ttctacaccc atattaggaa
      421 tatgtaagta accaaccttg ccatcggtag cagcacttac gcggtctata tttttttgca
      481 cccatgtata atattccaac ggtgcttgcg tgctaatggg cgttaccagt actttgcgag
      541 ctcccgttgc cgatgcttgc gaatttattt gcagctcaac ctgcttgcca actgtattca
      601 caaatgccac atttatatcg ttcagggtat ttgttggcat accattcacg gcaacaatgt
      661 agtcgccttc ttttgcattc acaccaatat cggtaagcgg gcagcgcaga ttgccggtcc
      721 agttttcgcc tttcaatatt tttgcaatgc gataatatcc atttgcttcg cgcaccaaat
      781 cggcacccaa caaccctagc tgtatacgtt cggcctttgg caaatcgcca ccgccaacat
      841 atgcatgccc aatgctgagc tcgccaatca tttcgccaat gatataagat aaatcggcac
      901 gatgatttac atatggcagc aatacttcgt acttagcttt catggcaggc cagtcaacac
      961 catgcatgtt gctatcgtaa aagaagtagc gcatttgtcg ccagctttca tcaaatattt
     1021 gtttccactc tgccttgcga tctacaagca cttttacatc acttgtattt atcgattctt
     1081 ttaaatccag tttccctttt ggcaaatcaa ttacataata agatttggtt ttacgtacca
     1141 acattttttt gttgttgttg cctatttcat aaccatcaat ttcgccaagg tctgtttcct
     1201 ttttatcttt aaggtcaaat aattttaacg cagttgcctc atctccattt ccgcgtttca
     1261 tgtaataaac gctattgtct ataattgaca atccaaaata acctgaagca gaaattggta
     1321 atacacctat gcggttttga attccatcga ggtcaatttt tacatctttg cttgcggcaa
     1381 ccttaggttc atctttcttt tttgcatcct tagttttgtc gtctgttgcc ggcttgctct
     1441 cttcagaatt tgtaattgct actgcatcgt tcaccggtgc aaacgggctt gtggtatttg
     1501 cagcaagtgt tacaaaatat attttactca tatcgctgta cgagtgattc cattcggtgt
     1561 tgctatatgt aggattgaaa tcgcgatcgg agataaagaa taaatatttg ccatcgctcg
     1621 aaaagcttgg cgaataacta tcgtaccact gcgtagttat gggagtaatt tttgcagttg
     1681 ccatttcgta caaatacaaa atgttttttt gcccaacttt actgtcgcta tagcttaccc
     1741 atttgctatc gctgctccat gtaaaatcat ttatttctcc tgtaacactt ttggcaattt
     1801 ccatcatatt tcctgacacc acatctaagc agttcaaccg cttttgttga tcgttccaca
     1861 atattttttt gctatcgggc gaccaaataa atccatactt atacgtgtcg gcattttttg
     1921 taagttgctt ggccgcctca gtagcatcag ctttttgaat atatattt
//
LOCUS       DAORQA010000329         1967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_12498_length_1967_cov_4.865063, whole genome shotgun sequence.
ACCESSION   DAORQA010000329 DAORQA010000000
VERSION     DAORQA010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12498_length_1967_cov_4.865063"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="PK736_09575"
     CDS             <1..1593
                     /locus_tag="PK736_09575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33676.1"
                     /translation="GINFTGTNAADFALLNAPAFPVNIAAGSFITITVKLTPSVLGTT
                     TAQLHIMNSDANESDYDVTLSGTGVCPVFASSISAGGPLTFCNGGSVNLTASNATSYL
                     WSNGATTQSIVASTSGSYYVIVSNTLGCTSQSPSTIVTITNTITITPAVIAVSCNGGS
                     NGVVALTVAGGTSPYTYLWSNSNTSATISSLTAGTYTVIVTDSLGCTSSLAVLVAQPA
                     VLATNFDGTQPASCGTSIGLGVANPSGGTSPYSYLWSNGVTTKTNPALSPDTYTVTVT
                     DSRGCTVSKDTTLADTKIYSATVNIVRPSCNGASDGSACVTGIVGGVGPFTYSWNTVP
                     IQTTACATGLVKGSYFVKITSAEGCNYISYITVTQPYVLIAAVNVVGKRATTLAQGGT
                     KAYSYLWSNGKTTKVAYNLPNGLYTVTVTDIKGCTTTATAVISVGVKESAFVQATENA
                     LNVFPNPTANEVSFSFASENDDTYNLTIIDVTGKEIYNENFATTQGMNEYRFDFAPYA
                     RGIYAIRISNKLNTMTSRVIVK"
     gene            1730..>1967
                     /locus_tag="PK736_09580"
     CDS             1730..>1967
                     /locus_tag="PK736_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33677.1"
                     /translation="MRASSKALITLVMAATTILFAAKFVSSETGISAITENQFIRDLK
                     SRVDKFMDFIPQDRVYLQFDKTLYQPGETIWFSAY"
BASE COUNT          595 a          406 c          395 g          571 t
ORIGIN      
        1 ggaataaact ttacaggcac caacgctgct gattttgctt tattgaatgc tccagcattt
       61 ccggttaaca tagctgcagg tagttttata actataacag tgaagttaac gccatctgta
      121 ttaggaacaa caactgcaca gctacacata atgaatagtg atgcaaacga atctgattat
      181 gatgttactt tatcgggaac aggggtatgc cctgtttttg caagcagcat ttcggcaggc
      241 gggccactta cattttgcaa tggcggaagt gtaaatctta cagcatctaa tgctacttca
      301 tatctatggt caaatggtgc cactacccaa tcaatagttg ccagcacatc gggtagttat
      361 tatgtaattg tttcaaatac ccttggatgt acatcgcaat cgccaagtac tattgtaact
      421 attactaata ctatcactat tactccggca gttattgcag tatcgtgcaa tggtggtagt
      481 aacggtgtag ttgccttaac ggtagcagga ggtacatcgc cttatacata cttatggagc
      541 aactcaaaca caagcgccac tatttcatca cttaccgctg gtacgtatac agtaattgta
      601 acagatagtt taggttgcac ttcgtcactt gcggtattgg ttgctcaacc tgctgtatta
      661 gctacaaatt ttgatggcac tcaaccggca tcgtgcggca ctagcatagg attgggtgtg
      721 gccaatccaa gtggaggtac ttcgccttat tcatacttat ggagcaatgg tgtaactacc
      781 aaaaccaatc ccgcactttc acccgatacg tatactgtta cagttacaga ctcgcgtggt
      841 tgcaccgttt cgaaagacac tacactggcc gatactaaaa tatatagcgc aactgtcaac
      901 attgtgagac cttcgtgcaa tggtgccagc gatggtagcg cttgtgtaac aggtattgtt
      961 ggtggagtag gaccttttac ttactcatgg aacacagttc ctattcaaac tacggcatgt
     1021 gcaacaggat tggtgaaggg ttcatatttt gtaaaaataa cttcggccga gggttgcaat
     1081 tatataagct acattacggt aacacaacct tatgttttaa tagcagcagt aaatgttgta
     1141 ggaaagcgtg caactacttt agcacaaggc ggaacaaaag cgtattccta cctatggtcg
     1201 aatggaaaaa ccaccaaggt agcgtataac cttcctaatg gattgtatac agttacagtt
     1261 accgatatca aaggttgcac cactacagcc actgctgtaa tatcagttgg agtgaaggag
     1321 tcggcttttg tacaagcaac tgaaaacgct ttaaatgtat ttcctaatcc tacagccaat
     1381 gaagtaagtt tctcgttcgc ctcggaaaat gatgatacat ataacctgac gataatagat
     1441 gtgacaggca aagaaatata caacgaaaac tttgccacca cacaaggtat gaatgaatac
     1501 agattcgatt ttgcacctta tgcccgaggt atatatgcaa taagaatttc gaacaaacta
     1561 aatactatga caagccgggt tattgtaaaa taataagcct tgtccatttt tagcagacct
     1621 gcctcttgat tgaggcaggt ttttttttgc tttcttttat ggaaacagaa taatcttttc
     1681 atctctctgt tgtaatcaca attttattaa tcaaataaaa cgctacaata tgagagcatc
     1741 atccaaagca ttaatcacct tagtaatggc ggccaccaca attttgtttg ctgccaaatt
     1801 tgtttcgagc gaaacaggca tttcggccat caccgaaaat caatttatac gcgacttaaa
     1861 aagccgtgtc gataaattta tggattttat tccccaagac agggtttacc tgcaatttga
     1921 caaaacgctt tatcagccgg gcgaaaccat ttggttcagc gcatatg
//
LOCUS       DAORQA010000330         1950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_12624_length_1950_cov_3.817942, whole genome shotgun sequence.
ACCESSION   DAORQA010000330 DAORQA010000000
VERSION     DAORQA010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12624_length_1950_cov_3.817942"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1087
                     /locus_tag="PK736_09585"
     CDS             <1..>1087
                     /locus_tag="PK736_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33678.1"
                     /translation="EQNVKSFADFNLHISLLGAQSQVPNIKADANLKTFEPYIYTGTD
                     TVWNELHEQAGLSFPISAKVLSPKVNVYELPKSGSTKLFVWKIKTEVEITEMEGDYFK
                     VRLRNESQTNVNEEANKYESAAEIPAPNAESRRNTQNDENVEQGWCKKSDLLLQGETV
                     TKEKILPPAIERVKIFFNKKLQNANRSFAKLSYRALLYIIKKEWRLPVIILLLVISIA
                     PLFIHQKEVVTEIYKNKEYLIDDDNKRAHFYLKKGYDCIIEKNAGAAFSWFVKSDSLI
                     KSDTIFNNLNVIAADAYDTDTINNVIQLYGDIEPNSIVGCRAWAYLKALSIGAKSMMQ
                     SMDKYSLLQQEMNAESKCFIKDFVLDS"
     assembly_gap    1088..1115
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1116..>1950
                     /locus_tag="PK736_09590"
     CDS             <1116..>1950
                     /locus_tag="PK736_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33679.1"
                     /translation="INWYGYFLNAYTSTHYALYQNGVDTNAFSQIATNFAIAYHKLLM
                     AYNADTGHFLAGYYNMNDYKIYVINTFPKIYTDSLKIIFGYTNPPVDNVAKVIYACNT
                     ITIKEFMKRFKATTEINGWDRQKRFINIAANKSMLYYATRNKSIYETYSISSICTNGI
                     SYRIIGKRNNRYKTFFVTPNQISRIGNNYISLTVCDNDVYPTMSDAELSDGSNCPVLS
                     IYPYKFKSNARACVYDNLNKRELSSEEETMLDQFILQMKESAASFISIKEFVFTPGVI
                     ST"
BASE COUNT          690 a          307 c          373 g          552 t
ORIGIN      
        1 cagaacaaaa cgtaaaatcc tttgccgatt ttaatttgca cattagtttg ctgggcgcac
       61 aatcgcaagt gccaaatata aaagcagatg caaatttaaa aacttttgag ccttacatct
      121 ataccggcac cgatacagtt tggaatgaat tgcatgagca ggcagggcta tcgtttccta
      181 ttagtgcaaa ggtgctgtcg ccaaaagtga atgtatatga gttgccaaaa tcgggaagta
      241 cgaaattgtt tgtatggaaa ataaaaaccg aagtagaaat aacggaaatg gagggcgact
      301 atttcaaagt gcggttgaga aatgaaagtc aaaccaatgt taatgaagaa gcaaacaaat
      361 atgaatcagc tgccgaaatt ccagcaccga atgctgaaag cagacgcaac acacagaatg
      421 atgaaaatgt agaacaaggt tggtgtaaga aaagtgattt gctgttgcag ggagaaaccg
      481 tgacaaaaga aaaaattctt ccaccggcta ttgaacgggt taaaatattt ttcaataaaa
      541 aattgcagaa tgccaatcgc agtttcgcaa aattaagtta tcgggcttta ctttatatca
      601 taaaaaaaga atggcggtta cctgttatta ttctactgct tgttatttcg attgcgcctt
      661 tattcataca tcaaaaagaa gtggttaccg aaatttataa aaacaaagaa tatttgattg
      721 atgatgataa taagcgggcg catttttatc tcaagaaggg ttatgattgc attatagaaa
      781 aaaacgctgg tgctgcattt agctggtttg tgaaatcgga tagcctcata aaaagcgaca
      841 ctatttttaa taacctgaat gtgatagccg ctgatgcata cgatactgat actataaaca
      901 atgtgataca attgtatggc gatatagaac cgaacagcat agtaggttgc cgcgcatggg
      961 cttatctcaa ggcattgagc ataggtgcaa aaagtatgat gcaatcgatg gataagtata
     1021 gtttgctgca gcaagaaatg aatgctgaaa gcaaatgctt cattaaagat tttgtattag
     1081 atagtaannn nnnnnnnnnn nnnnnnnnnn nnnnnatatt aactggtatg gatacttttt
     1141 gaatgcttac acatcaactc attatgcatt gtatcaaaat ggcgtagaca caaatgcatt
     1201 ttcacagatt gcaacaaatt tcgccattgc ataccataaa ttattgatgg cttataatgc
     1261 agacacgggt cattttctgg cagggtacta caatatgaat gattataaaa tttatgtgat
     1321 aaatactttt cctaaaattt ataccgactc attgaaaatt atttttggtt acacaaatcc
     1381 tcctgtcgac aatgtcgcaa aagtaattta tgcatgtaat acaattacaa taaaggaatt
     1441 tatgaaaaga tttaaggcca caaccgaaat taatggttgg gacaggcaaa agcgtttcat
     1501 aaacattgct gcaaataaaa gtatgctgta ttatgcaact agaaacaaaa gtatttacga
     1561 aacatattct ataagttcca tttgtaccaa tggaataagt tatagaataa tagggaaacg
     1621 aaataacaga tacaaaacat tttttgtgac tccaaatcag atttcacgaa ttggcaacaa
     1681 ctacatttct ttgacggtat gcgataatga tgtgtatcca actatgtccg atgccgaatt
     1741 gagcgatggc agtaattgtc cggttcttag catctatcca tataaattta aatcgaatgc
     1801 ccgtgcatgc gtttacgata atttgaacaa aagagagctt agcagcgaag aggaaaccat
     1861 gctcgatcaa tttattttac aaatgaaaga atctgccgca tcatttattt cgattaaaga
     1921 attcgtattt acgcccggtg ttatcagtac
//
LOCUS       DAORQA010000331         1938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_12721_length_1938_cov_3.899097, whole genome shotgun sequence.
ACCESSION   DAORQA010000331 DAORQA010000000
VERSION     DAORQA010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12721_length_1938_cov_3.899097"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>355
                     /locus_tag="PK736_09595"
     CDS             <1..>355
                     /locus_tag="PK736_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRC33680.1"
                     /translation="DEIGNITLQQQSKLLSVLQNRQVTKLGSNQASSIDIRLICATNL
                     PLSELANETRFRKDLIYRINTISITAPPLRERGDDAVLLAKHFISLYGKKYFKTDLQL
                     DDKSIAKLKSYTFPG"
     assembly_gap    356..408
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <409..628
                     /locus_tag="PK736_09600"
     CDS             <409..628
                     /locus_tag="PK736_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC33681.1"
                     /translation="EGNTITPGDLLFSPIENVNQNSVQSDDLKLSSLEKNTIMHVIEK
                     NNGNISKAAKELGITRTALYRRLSKYDI"
     gene            618..>1938
                     /locus_tag="PK736_09605"
     CDS             618..>1938
                     /locus_tag="PK736_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRC33682.1"
                     /translation="MIFKNFKHRFLLAIFLLALVIIGACLIYFAWKKWWAMLFIPLIA
                     YQIYDLYQYLNKAYEDVDEFVQSVHYRDFTKNFNLKRSPLELQQLRSGFNEINSAFKV
                     ISLEKETQFQYLQKVLEIVNTGIISFNIETTEVNWVNDTFKKMINVPYLKTINSLEKR
                     NEKLYKSIINIKAGESSLLNVEHQNHTLKLLLSATVFISEEKTNKIIAFQKVNQAIDE
                     TEADAWKKLLSVMTHEIMNSIAPISSLADTLKGNIANAPEALRKTYTDLDDIEIGLET
                     IKRRSEGLLRFAEVYRNLNKITQLNLKKIFVREMFSNIHNLMLPTLEQKNIELEVILP
                     NPQLSIELDPHLIEQVLINLILNASEAVKEQHKAKITLSAYQENNNNIIYVRDNGIGI
                     SAEVLEKIFVPFFSTKKSGSGIGLSLSKQIMLLHKGSIYVNSKVDDGT"
BASE COUNT          698 a          325 c          349 g          513 t
ORIGIN      
        1 tagatgaaat tggcaacatc acgttgcaac agcaatcgaa attgttatcg gtattacaaa
       61 acagacaagt taccaaactg ggcagcaatc aagcatcaag tatagacata cgtttgatat
      121 gtgccaccaa tttaccactg agcgaactgg caaacgaaac acgttttcgc aaagatttga
      181 tttaccgcat caacactata agtataacgg caccaccact gcgcgagcgt ggcgatgatg
      241 cagtgctatt ggcaaaacat tttatcagct tgtatggaaa aaaatatttc aaaaccgatt
      301 tgcaattaga tgataaaagt attgcaaaat taaagtcgta tacattcccc ggtaannnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg aaggaaacac
      421 aatcaccccg ggcgatttac ttttttcacc tatcgaaaac gtaaatcaaa acagtgtgca
      481 aagcgatgat ttgaaattga gctcactcga aaaaaatact atcatgcatg tgatagaaaa
      541 aaataatggt aatatatcga aggccgccaa agaacttggt attacacgaa cagcactgta
      601 tcgcagacta agtaaatatg atatttaaaa atttcaaaca tcggttcctg cttgccattt
      661 ttcttttggc actggtaatt attggagcct gcttaattta ttttgcgtgg aagaaatggt
      721 gggccatgct ttttattccg ctcattgctt atcaaattta tgatttgtat caatacctta
      781 ataaggctta tgaagatgta gatgagtttg tgcaatctgt tcactatcgt gactttacaa
      841 aaaactttaa tttgaaaagg tcgccactcg agttgcaaca actgcgtagc gggttcaacg
      901 aaattaattc ggcattcaaa gttatcagtc tcgaaaagga aacacaattt caatacctgc
      961 aaaaagtttt agagattgta aacaccggaa tcatttcgtt caatatagaa actaccgaag
     1021 tgaactgggt taacgataca ttcaaaaaaa tgataaatgt gccatatttg aaaactataa
     1081 attcgcttga aaaacgaaat gagaaacttt ataaaagcat tatcaatata aaagccggag
     1141 aaagtagttt gctgaatgta gaacatcaaa accacacatt aaaattactt ctatcggcaa
     1201 cagtatttat atctgaagaa aaaacaaata aaataatcgc atttcaaaaa gtgaatcagg
     1261 ccatagatga aaccgaagcc gatgcgtgga aaaaattatt gagtgtaatg acacatgaaa
     1321 taatgaatag cattgcgccc atatcatcgt tggccgatac actgaaaggc aatattgcca
     1381 atgcacccga agcattgcgc aaaacctata ccgatttgga tgatatagaa attgggctcg
     1441 aaacaataaa gcgaagaagc gaagggttgc tacgttttgc cgaagtgtac cgcaatctta
     1501 ataaaatcac acaactcaat ttgaagaaaa tatttgtgcg cgaaatgttt tcaaatattc
     1561 ataatttaat gttgccaact ttggaacaaa aaaatattga gctcgaagta attttaccaa
     1621 atccacaact aagcattgag ctggacccgc atcttattga gcaagtgctt ataaatttaa
     1681 tactcaatgc aagcgaagca gtgaaggaac agcacaaggc caaaattact ttaagtgctt
     1741 atcaggaaaa caacaacaat ataatttatg tgcgtgataa tggtattgga ataagtgccg
     1801 aagtattgga aaaaatattc gtgccttttt tctctacaaa gaaaagtgga agtggcattg
     1861 gattaagttt gtcgaaacaa attatgttgc tgcacaaagg ttcaatttat gtaaacagca
     1921 aagtggatga tggtacag
//
LOCUS       DAORQA010000332         1937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_12724_length_1937_cov_5.649841, whole genome shotgun sequence.
ACCESSION   DAORQA010000332 DAORQA010000000
VERSION     DAORQA010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12724_length_1937_cov_5.649841"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..>1063)
                     /locus_tag="PK736_09610"
     CDS             complement(59..>1063)
                     /locus_tag="PK736_09610"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HRC33683.1"
                     /translation="KVVGSAADIPDEDTIEKGNIIYVVITIEGAHSFGVSPNPESKPT
                     IDTTTVLANIAAVKKWKQRPFFVTLSHHFYNELAGHARSLKIPKEILGIKLSGIDQEF
                     GINTGITAFGFEVIDAMLDNKNNKRILVDIKHMSRLSRTMYFNYLDEHYNTDDSKIPI
                     VVSHGGVAGIKSDGSETIAGAKKRFFNDDINIYDDELIYVCKSGGILGIQLDERRIAS
                     ADVLHGFAIKYLDNDAEEWSRLVWAQIQHAAEVFDNAGLPAWRTLCIGSDFDGIINPI
                     GPFATCAEMPLLYDPLIKYATQFMKGNTLKKNFNKVSPRDIVDAFMYGNAFNFLKMHY
                     "
     assembly_gap    1064..1073
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1074..1513)
                     /locus_tag="PK736_09615"
     CDS             complement(<1074..1513)
                     /locus_tag="PK736_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33684.1"
                     /translation="MAKLIFDIHCHPSFKAFGQSFKNGASEGNTTNAKKKNSVWYKSK
                     LNRRRELFFGATPYTQSDFTSVSQGITKLIVASLYPFEKRFVVDKNGVNTTDLGMQNF
                     IIGIGKERIRYLRNLDDYFFDLNQEYNYLCAIEKQPVTIDGKEY"
     gene            complement(1595..>1937)
                     /locus_tag="PK736_09620"
     CDS             complement(1595..>1937)
                     /locus_tag="PK736_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="patatin"
                     /protein_id="HRC33685.1"
                     /translation="MLMQDASWANQTILQWLSRSPTAKSIDGEVGDLTEDLISPEPIL
                     HYLRYNVTVAAAELEKITGKQYTTEHVTRLTDMSDAKNCAELYEIGYAAGQKEILDSH
                     IPAAFDLKISA"
BASE COUNT          530 a          384 c          360 g          653 t
ORIGIN      
        1 tttatatgca agggtttgag gagactatca ccaactattt ttgaccatta tatcgttttt
       61 aataatgcat tttcaaaaaa ttaaatgcat tgccatacat aaaagcatca acaatatcgc
      121 gcgggcttac tttattgaaa tttttcttta aagtatttcc tttcatgaat tgagtagcgt
      181 attttattaa tggatcgtaa agcagaggca tttcggcaca ggtggcaaag ggtcctatag
      241 gatttattat tccatcgaaa tcggagccta tgcacagtgt gcgccaagcc ggaaggccgg
      301 cattgtcgaa cacttcggca gcatgttgta tttgtgccca cacaagcctg ctccattctt
      361 cggcatcgtt gtcgaggtat tttatagcaa agccatgcag cacatcggca ctggcaattc
      421 gtctttcgtc aagttgtata ccgagtatgc caccactttt acaaacgtat atcaactcat
      481 cgtcataaat atttatgtca tcgttgaaga atcttttctt tgcacctgca attgtttcgc
      541 tgccatcgct ttttattccg gcaacaccac catgacttac tacaatcgga attttactgt
      601 cgtcagtatt gtagtgctcg tcaagataat tgaaatacat ggtgcgcgat aaacggctca
      661 tgtgttttat atctaccaaa atgcgtttgt tgtttttatt atcgagcatg gcatctatca
      721 cttcgaaacc aaaagcagta attccggtat taataccaaa ctcctgatcg atacccgaca
      781 attttattcc caaaatttct ttgggaattt ttaaacttcg tgcatggcct gcaagctcat
      841 tataaaagtg atgcgatagc gtaacaaaaa atggacgttg cttccacttt tttacggcag
      901 caatatttgc caatactgtt gttgtatcga tagtcggttt actttcggga ttgggactca
      961 caccaaagga atgcgcaccc tcaattgtaa tcaccacata aattatattt cctttttcaa
     1021 tagtgtcttc atcgggaata tcggcagcac tgcccaccac tttnnnnnnn nnnatatatt
     1081 ctttgccatc tattgttaca ggttgctttt cgatagcgca taaataattg tattcctgat
     1141 tgagatcgaa aaaataatca tcaagattgc gcaagtaacg gatgcgttct ttaccaatac
     1201 caattataaa attctgcatg ccgaggtcgg tggtgttcac accattttta tctacaacaa
     1261 atcttttttc gaaagggtac aaagaggcca caatcaattt tgtgatgcct tgacttaccg
     1321 aagtaaaatc gctttgtgtg tagggcgtag cgccaaaaaa taattcacgc ctgcggttga
     1381 gcttggattt gtaccaaacg gaatttttct tttttgcatt ggtggtattg ccttccgatg
     1441 caccgttttt aaaactttgt ccaaatgctt tgaacgatgg atgacaatga atgtcgaaga
     1501 tgagtttagc catggttatt tttttttatt ggttagaaat gtgaatatga gcgttttgtt
     1561 ctttactttt ttttaaacaa taatttcata attattatgc acttattttc aaatcgaaag
     1621 cagcaggtat gtgactatcg agtatttctt tttgccctgc ggcatatcct atttcgtaaa
     1681 gttcagcaca gttttttgca tcactcatat cggttagcct ggttacatgt tcggtagtat
     1741 attgtttgcc ggtaattttt tccagctcgg cagcagccac tgtaacattg taacgtaagt
     1801 aatgtaaaat tggttcgggt gaaattaaat cttcagttaa atcacctacc tcaccatcta
     1861 tactttttgc tgtgggtgag cgcgataacc attgcaaaat tgtctggttg gcccagcttg
     1921 catcctgcat caacata
//
LOCUS       DAORQA010000333         1931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_12766_length_1931_cov_6.082623, whole genome shotgun sequence.
ACCESSION   DAORQA010000333 DAORQA010000000
VERSION     DAORQA010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12766_length_1931_cov_6.082623"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PK736_09625"
     CDS             complement(<1..863)
                     /locus_tag="PK736_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRC33686.1"
                     /translation="MILVYTENTNGKFKKSTYEALSYAKAIGQKLNVDVAAISFGTTD
                     NDELKNLAQYGANKIFQVINSKFQSFNGQTYASAIAEAALQNNCSVVVIGNSFSGKGL
                     APRVAIKLNAGFVDGAISLPDTSNGFVVRKGAYSGKAFADVNVLAATKVISLVPNSYK
                     VETNPCETNIEEFNPTVKESDFKLTIKETVRAGDKISLPEAELVVSAGRGLKGPENWG
                     MVEELAGLLGAATACSKPVSDSHWRPHSEHVGQTGIAISPNLYIAIGISGAIQHLAGV
                     SSSKTIVVINK"
     gene            complement(899..1636)
                     /locus_tag="PK736_09630"
     CDS             complement(899..1636)
                     /locus_tag="PK736_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRC33687.1"
                     /translation="MNILVCISNVPDTTSKINFNDDNKGLNTAGLQFIINPYDEIALT
                     RALELTEKAGGTVTVINVGDASTEPTIRKALAIGATDAVRINAAPRDAFFVASQIAHF
                     AKGKNYDLILCGRESIDYNGAQVTAMIAELLDLPAITIVKMLEINGNTATLEREIEGG
                     KEIVDCPLPLVAGASEGMAEPRIPNMRGIMSARTKPLVAVEPIAIDQLTEIVGYDKPK
                     PRSTVNLISSDDVDKLVQLLHTEAKVI"
BASE COUNT          551 a          386 c          364 g          630 t
ORIGIN      
        1 tctttattta ttaccactat tgttttggaa gaacttaccc cggctaaatg ttgaatcgct
       61 cccgaaattc caatagcaat atatagattc gggcttattg caataccggt ttgcccaacg
      121 tgctcactat gtggtcgcca atgcgaatcg ctcacgggtt tggaacatgc cgttgcggca
      181 cccaacaaac cggccagttc ttcaaccata ccccaatttt cgggaccttt aagtcctcgc
      241 ccggcactta ctaccagttc ggcctcgggc agcgatattt tatcgccagc gcgcacggtt
      301 tcttttattg ttaatttaaa atcgctttct tttactgttg gattaaattc ctcaatgttt
      361 gtctcacagg gattggtttc taccttatat gaatttggaa cgaggcttat aactttagta
      421 gctgccagca catttacatc agcaaatgct ttgccggaat atgccccttt gcgcactaca
      481 aaaccattcg aagtatcggg taatgaaata gcaccatcaa caaacccggc atttagtttt
      541 attgcaacac gtggtgctaa cccttttccc gaaaacgaat tgccaatgac taccacactg
      601 caattatttt gcaaggctgc ttctgcaatt gctgatgcat aggtctgacc attgaatgat
      661 tgaaatttcg aattgatgac ttgaaaaatt ttattggcac catattgtgc taagtttttt
      721 aactcgtcat tatctgttgt accaaacgaa atagctgcaa catcaacatt taacttttgg
      781 cctattgctt ttgcatatga aagcgcttca taggtcgatt ttttaaattt cccgtttgtg
      841 ttttctgtat atacaagtat cattttattt tttttgtaaa atctgtttta aataattact
      901 aaataacctt ggcttcggta tgaagaagtt gcacaagttt gtctacatca tcagaagaaa
      961 ttaagttaac ggttgaacgt ggcttaggtt tatcataacc tacaatttct gttaactgat
     1021 cgatggcaat tggttcaact gcaaccaagg gtttagtacg tgcactcatt attccacgca
     1081 tatttggtat acgcggttcg gccattcctt ctgaagcacc tgccaccaat ggtaaagggc
     1141 agtcgacaat ttctttgccg ccttctattt cgcgttcaag agttgctgtg ttgccattta
     1201 tttcgagcat ttttacaata gtaatcgcag gcaaatcgag cagttcggca atcatggcag
     1261 taacctgagc gccattataa tctatcgatt cgcgaccaca taaaatgaga tcgtaatttt
     1321 ttcctttggc aaaatgagca atttgcgaag ccacaaaaaa tgcatcgcgt ggtgcagcat
     1381 ttattcttac tgcatcggtt gctccaatgg ctaatgcttt gcgtatggtg ggttcggtag
     1441 aggcatcgcc aacattaata acggttacag tgccaccggc tttttcggta agttccaatg
     1501 cgcgggtgag tgcaatttca tcgtatgggt taatgataaa ctgcaaacct gcggtgttca
     1561 ggcctttatt atcgtcatta aaattaattt ttgaagtggt atcgggcaca ttgctgatac
     1621 aaactaaaat attcattgtt aaaaaaaatt ggtcggcaaa gctaacctaa atgtttgagt
     1681 aagtcaaagg aacaagctgc tatatttata ttattttagc taggtgtata actataagaa
     1741 attgctttaa ccataaatgt agtaaacaag caaaatttaa ttttttaatt caatgggttc
     1801 ctgtttttac aaattatgtg aagcaatcga taataaataa aatcggtgtg aagtgtcttc
     1861 tatcaattga aataaaaaaa atcaatatta cacctcgcgc ttcataagtt ggcttgcgaa
     1921 tagctgtaaa a
//
LOCUS       DAORQA010000334         1928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_12782_length_1928_cov_5.096636, whole genome shotgun sequence.
ACCESSION   DAORQA010000334 DAORQA010000000
VERSION     DAORQA010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12782_length_1928_cov_5.096636"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>377
                     /locus_tag="PK736_09635"
     CDS             <1..>377
                     /locus_tag="PK736_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33688.1"
                     /translation="QIPVGIQIKNMGASSTNSMQIKYELDGQLLNTYNYNSVLMYNEY
                     QYLGLPSFTTTAGPHVFKVYIDWPSDSNHNNDTLVLNFNAVNATLQGLPYQTDFETTV
                     GTWYNANFSFANNNWQLGTPAYG"
     assembly_gap    378..387
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <388..>1928
                     /locus_tag="PK736_09640"
     CDS             <388..>1928
                     /locus_tag="PK736_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33689.1"
                     /translation="TSGAHSGTNSWDINLNSPYVPDGLSCLYSPVFDFTNAITPKLKF
                     WLNYNMDFTDGAFLQYSTNGTTWNMLGILNDPNGINWYNQQLYYFNNIPGWALQTTGW
                     TFHQYDLYGLNLSAPVQFRYGFYSSQYGNGDGISLDDFEIELALQNDAQLKTINEPGA
                     IVTSGIALNPVVTIRNNGSVPLTSLLLNYDVNNVYTNSYTWTGNLPYDSVATVTLPAY
                     TPSPGLSNIKVYSTWNADLNAYNDTLKNNYTSIVSANAPYIDNFDNAQFSWIPTSTPI
                     YFNEWQYGTPNYGSTMGCFTGSNCWDLNLNLAYNNLANAKLFSPYFNYSTIVNSKLGI
                     YLNFDTEYGADGLRIEYTTNGTTWSVLGSINEPGSGNWYNSNLSDGKPGWSYQSQGWK
                     HAYISTPYLDGKNLVQFAFVFKSDFSITGIGATIDDFEISPAAGILENTKFNANIFPN
                     PFNNKLNINLPAYIQHGNIVVKDLFGKTVEVKNITSTNIEFDTTEWNAQTYFIHITSD
                     ANTSV"
BASE COUNT          600 a          397 c          352 g          569 t
ORIGIN      
        1 gacaaatacc tgttggcata caaataaaaa atatgggtgc aagcagtacc aatagcatgc
       61 aaataaaata cgagctcgat ggtcaacttt taaatactta caactacaat agtgtcttaa
      121 tgtataatga gtatcagtac ttaggcttgc cttcatttac tactacagca ggtcctcatg
      181 tttttaaagt atatatcgat tggccatccg attcgaacca taacaacgat acactggttt
      241 taaattttaa tgccgtaaat gcaacattgc aagggttgcc gtatcaaaca gattttgaaa
      301 ctacagttgg tacgtggtac aatgccaatt ttagttttgc taataataac tggcaattgg
      361 gaactccggc ttatggcnnn nnnnnnnaac aagcggtgca cactcaggca ctaatagttg
      421 ggatataaat cttaattcgc cttacgtgcc cgatggcttg agttgcttat actctcccgt
      481 tttcgatttt accaatgcca ttacgcccaa gttaaagttt tggctcaact acaacatgga
      541 ttttaccgat ggagcatttt tacaatattc taccaatggc actacatgga atatgctggg
      601 catattaaat gaccctaacg gaatcaactg gtacaatcaa cagttatatt atttcaataa
      661 catacccgga tgggcgttgc aaactaccgg atggactttt catcagtacg atttatatgg
      721 tctcaattta agcgcgccgg tacaatttcg ttatggtttt tatagctcac agtacggtaa
      781 cggtgatggc atttcgcttg acgatttcga aattgaactt gccctgcaaa acgatgcaca
      841 gctaaaaacc attaatgaac ccggtgctat tgttacttcg ggcatagcat tgaaccctgt
      901 ggtaaccatc cgcaacaatg gcagtgtgcc cttaacatcg ctattgctaa actacgatgt
      961 gaacaacgta tacacaaata gttatacatg gactggcaac ctgccttacg attcggtggc
     1021 taccgttact ttgccggcat acacaccttc gcccgggctt agcaatatta aagtttactc
     1081 tacatggaat gccgatttaa atgcctacaa cgataccctc aaaaataatt atacttctat
     1141 tgtatcggca aatgcacctt atatagataa tttcgataat gcacaatttt cgtggatacc
     1201 tacaagtaca cctatatact ttaatgaatg gcaatacggc acaccaaact atggcagcac
     1261 tatgggttgc tttacaggct ccaattgttg ggatttgaat ttaaaccttg cctataataa
     1321 tctggcaaat gcaaaactgt tttcgcctta ctttaattac agtactatag taaatagtaa
     1381 gttgggaatt tacctcaact tcgataccga atatggtgcc gatggtttac gcatagagta
     1441 caccaccaat ggcaccacat ggagtgtgct tggcagtata aacgagccag gtagtggtaa
     1501 ttggtacaac tcaaatttaa gtgatggcaa accaggatgg tcgtatcaat cgcagggatg
     1561 gaaacatgct tacatcagca caccttatct cgatggaaaa aatttggtgc agtttgcttt
     1621 cgtttttaaa tccgattttt ctattacagg cattggtgcc acaatagacg atttcgaaat
     1681 ttcgcccgct gccggtattt tggaaaatac aaaatttaat gccaacattt tccccaatcc
     1741 attcaacaac aaattgaata ttaacttacc tgcgtatatc caacatggta atattgtagt
     1801 gaaagatttg tttggcaaaa cggttgaggt aaaaaacatc acctcaacca atatagaatt
     1861 tgataccaca gaatggaatg cccaaactta ttttattcac atcacttccg atgcaaatac
     1921 cagtgtat
//
LOCUS       DAORQA010000335         1902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_12993_length_1902_cov_5.492691, whole genome shotgun sequence.
ACCESSION   DAORQA010000335 DAORQA010000000
VERSION     DAORQA010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12993_length_1902_cov_5.492691"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PK736_09645"
     CDS             complement(<1..516)
                     /locus_tag="PK736_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33690.1"
                     /translation="MTVFLSIVRFSKFLKVLTACVILWCCLISIADAQLNYSRSTFDA
                     AYVPITLANGAVQIVGGSGANNFSTTTGASNLDDGTAYISLPFNFNYAGTIFTSSHFL
                     AISTNGFLYFSTVNTNFSKISIFDNTRIFGTLAPNNCLAPYFDDLKLGAVGTNAAGEV
                     LYKIFNATATNE"
     gene            complement(741..1640)
                     /locus_tag="PK736_09650"
     CDS             complement(741..1640)
                     /locus_tag="PK736_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRC33691.1"
                     /translation="MLQKFNPQNENIFVFVKDKLYQRNEAKVSVFDSSVQGGDAVWEG
                     LRVYNNKIMSLNEHLDRIEASAHALQFNNVPSREFIIDAIKQTLAANKMDNNVHIRLT
                     LTRGEKITSGMDPRLNTNGCCLIVLAEWKPPVYDNETGVSLVTAHIRRNGPQFLDSKI
                     HHNNLLNNILAKIQANLMNADDALMLDNNGFVSETNACNIFMIKNDVVYTPHADSCLP
                     GITRSTVINICNKNKIMCVERNLSLTEFYNADVVFTTGTMGELTKVNLIDGRNIVNKK
                     SPGILLQLQELFKEVISQNAVAI"
     gene            complement(1644..>1902)
                     /locus_tag="PK736_09655"
     CDS             complement(1644..>1902)
                     /locus_tag="PK736_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRC33692.1"
                     /translation="ENPEHALTQLCNALDIPFDNAMLKWPVGPKKEDGCWAPYWYTNI
                     HKSKGFEKSENKNITVLPQHELLLEECMMHYNKLKSYEIKL"
BASE COUNT          591 a          349 c          351 g          611 t
ORIGIN      
        1 ttcattggtg gctgttgcat taaatatttt gtataatact tcacctgccg cgttggtacc
       61 aactgcacct aattttaagt cgtcaaaata tggtgcaagg caattgttgg gagccaatgt
      121 gccaaaaatt cgggtattat caaatataga aattttcgaa aaatttgtat ttacggtaga
      181 gaagtataaa aatccattgg tgcttattgc caaaaaatgg gatgaagtaa atatagtacc
      241 ggcataatta aaattgaatg gcaatgaaat gtatgccgtt ccatcatcga ggttagaagc
      301 acctgtagtg gttgaaaagt tatttgcacc actaccacct acaatttgca cagcaccatt
      361 ggctaaagtg ataggaacgt atgccgcatc gaaggtgcta cgtgaatagt ttagctgtgc
      421 atcagcaatg gaaattaaac aacaccagag tataacacaa gctgttaaga ctttgaggaa
      481 tttcgaaaac cggacaatgg ataaaaatac tgtcatgaca aaaaataatt acaaaagcag
      541 cgcgaaaaat cttactgcgg ttaaaaaaaa tagcttctac aaattagaga agagtacaaa
      601 ttatgcaatc tgaaaaattt actgattgct tcataaaaaa ttgaaacaca aagaaaggtt
      661 tataaatcaa aagtgtaaag cttcaagtct taaatcttgt ttgctttttg ggcgaaattt
      721 cggatttgat aaattaaata tcaaatcgcc actgcatttt ggctaatgac ttccttaaat
      781 aattcctgaa gttgtaataa tatgccgggc gattttttat ttacaatatt ccttccatca
      841 atcaggttaa cctttgtcaa ctcgcccatg gtaccggttg taaataccac atcggcatta
      901 taaaattcgg tgagcgacaa atttctctcc acacacatta ttttattttt attgcaaatg
      961 tttatcaccg ttgagcgtgt aatgcccggc aaacagctat cagcatgtgg agtatatacc
     1021 acatcgttct taatcatgaa aatgttgcat gcattggttt cagatacaaa gccattatta
     1081 tcaagcatca aagcatcatc ggcattcatg aggttggcct gaatctttgc aagaatatta
     1141 ttcaataaat tattgtggtg aattttcgaa tccaaaaatt gtggtccatt acgtcttatg
     1201 tgagccgtta caaggcttac acctgtttca ttatcataca caggaggctt ccattcggca
     1261 agtacaataa ggcaacaacc gttagtgttt aatcgcgggt ccatgcccga ggtaattttt
     1321 tcgccacggg taagtgtcaa tctgatgtga acattattat ccattttatt ggcggccaat
     1381 gtttgcttta tggcatcaat gatgaattcg cgcgatggaa cattattaaa ctgtaatgca
     1441 tgtgccgaag cctcaattct atctaggtgt tcgttcagcg acattatttt attgttgtac
     1501 acacgtaatc cttcccacac ggcatcgcct ccctgcacac tgctatcgaa taccgaaacc
     1561 tttgcttcat tgcgctggta taatttgtct tttacaaaca cgaaaatgtt ttcgttttga
     1621 ggattaaatt tctgcaacat gttttataat tttatttcgt acgattttaa tttattgtag
     1681 tgcatcatac attcttccaa cagcaattcg tgttgaggta aaacggtgat gtttttattt
     1741 tcgctctttt cgaaaccctt cgatttatga atattagtgt accagtatgg tgcccagcaa
     1801 ccgtcttctt tttttgggcc aacaggccat ttcagcatgg cattatcgaa tggaatatca
     1861 agggcattgc ataattgcgt taatgcatgt tcaggatttt ca
//
LOCUS       DAORQA010000336         1895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_13053_length_1895_cov_8.415217, whole genome shotgun sequence.
ACCESSION   DAORQA010000336 DAORQA010000000
VERSION     DAORQA010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13053_length_1895_cov_8.415217"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1732
                     /locus_tag="PK736_09660"
     CDS             <1..1732
                     /locus_tag="PK736_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33693.1"
                     /translation="TWIALNIPDLGGIESISIPHPDTLFAITNTNFIISSFDGGQTFT
                     SYFLEIGNGNAERRISMLNSQVGYIGATQGHIFKTTNSGSCWQKVFTVANQQCNAIAV
                     TPTKDQIYFAYGYDDSTAVIYNFQSYHKLIATVDAAQCSFQPVCTHNLSTGYTSFKWY
                     LDNVFITNSRDTCITFTNAGAHILKLVADSANIIVDSISFPINVLNGSSSVKPLTGDS
                     AICSNYQILSGSNHDYFVQGNASIVTYHWYSDLPNAIFYGLQGETDSLASVYYAPNPN
                     FTNTLNIFVYGETAAGCLSDTASIHVVLNHALPPDATNLTLADDCLSELTSSFSSGLP
                     VDSIFCTTTNQLSKFALYYNFSGLPPTTQPSVYLPVYAQCPGQQSVALYLANGCGIST
                     NPKVANYNLEYLPQSIVNTPDTIVKGGAMFDILFYPQYDADVMINCTTPDIVWFKDGM
                     PTGNFGNTYPIPYASQFDSAMYTAAIINLCDTIFTNILIVVDVAASTNEITESNFKII
                     DDGKIVRIQNPYFETFNLQLINMLGDKIGSYKINYGMNTFDLSALAPQLYILQANSKS
                     DFIKYKLLRR"
BASE COUNT          597 a          365 c          324 g          609 t
ORIGIN      
        1 aacatggata gcactaaata tacccgactt aggtggaatt gagagtattt cgataccaca
       61 tcccgatact ttatttgcta tcaccaacac aaattttata atttcaagct tcgatggtgg
      121 gcaaacattt acgagttatt ttttggaaat aggaaacggt aacgcggaaa ggagaatttc
      181 tatgcttaat tcgcaagttg gttacatagg agcaacacaa ggccatattt ttaaaacaac
      241 aaattctggt tcatgttggc aaaaagtatt tacggttgcg aatcaacaat gcaatgcgat
      301 tgcagttaca cctacaaaag accaaatata ttttgcttat ggttatgacg actcaactgc
      361 agttatctac aattttcaat cttatcacaa attaattgcc acagttgatg ctgcacaatg
      421 ttcttttcag cctgtttgca cacacaacct aagcacaggt tatacgtcat tcaaatggta
      481 tcttgacaat gtgttcatca ccaattcgcg cgatacatgt attacattta ctaatgctgg
      541 cgcacatata ttaaagcttg ttgccgacag tgcaaatatt attgtcgata gtatttcgtt
      601 tcctatcaat gttttgaatg gttcatcatc cgtaaagcca cttaccggtg attctgctat
      661 ttgtagtaat tatcaaattt tatcgggatc aaatcatgat tattttgtcc aaggtaatgc
      721 atcaatcgta acttatcatt ggtacagcga tttgccaaat gcaatatttt atggtttgca
      781 aggtgaaaca gactcgttgg catctgtgta ttatgcaccc aacccaaact ttaccaatac
      841 attaaatatt tttgtctatg gcgaaacagc agcgggatgt ttgagcgaca ctgcatcaat
      901 acatgttgtt ctcaatcatg ctttacctcc cgatgcaacc aaccttacat tagccgatga
      961 ttgcttgtcg gagcttacaa gcagttttag ttcggggctg ccagtcgatt ctatcttttg
     1021 tacaacaaca aatcagcttt cgaaatttgc tttgtactac aacttttcag gattgccgcc
     1081 cacaactcaa ccgagcgttt atcttcctgt atacgcgcaa tgccccggcc agcaaagtgt
     1141 tgcgctgtat ctcgcaaatg gctgcggtat atcaaccaac cccaaagttg caaactacaa
     1201 tttggaatat ttgccgcaaa gcattgtaaa cactcccgat acaatagtaa agggtggcgc
     1261 catgttcgat attttatttt acccccaata tgatgcagat gtaatgatca attgcacaac
     1321 accagacatt gtttggttta aagatggaat gccaacagga aattttggca atacttatcc
     1381 aataccttat gcatcgcaat tcgactctgc tatgtatacc gcagcaatca tcaatctttg
     1441 cgataccata ttcacaaata ttttgatagt ggttgatgtg gctgcatcaa caaacgaaat
     1501 aacagaatcg aattttaaaa ttattgacga tgggaaaatt gtgcgaatac aaaatcccta
     1561 cttcgaaaca tttaatttac aactgataaa tatgttgggg gataaaatag gctcgtacaa
     1621 aattaattat ggaatgaata cgtttgactt atcggctctt gcaccacaac tctacatctt
     1681 acaggcaaat tcgaaaagtg attttataaa gtataagtta ttgaggaggt gatttttttt
     1741 gaagaattta acgcagcgtt ttattgttcg actgtagctt gaattctttt tattaaataa
     1801 ttttgattgc taaaaaagat atatttgtaa aaacatttac atcatgcaaa acaaaattat
     1861 tttaatcttc attcttttaa gccacacatt atttt
//
LOCUS       DAORQA010000337         1886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_13126_length_1886_cov_4.351174, whole genome shotgun sequence.
ACCESSION   DAORQA010000337 DAORQA010000000
VERSION     DAORQA010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13126_length_1886_cov_4.351174"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /gene="tyrS"
                     /locus_tag="PK736_09665"
     CDS             <1..1232
                     /gene="tyrS"
                     /locus_tag="PK736_09665"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRC33694.1"
                     /translation="RNMLHDVMPGTEEQLNKEMTTGYIGFDPTADSLGVGNLVQVMTL
                     LHFQRCGHKPIALVGGATGMVGDPSGKSAERNLLSEDILNHNLDSQKKQLEKFLNFDC
                     GANSAEIVNNYDWFKDFTFLDFIRDVGKHITVNYMMAKDSVKSRLDTGMSFTEFSYQL
                     VQGYDFVHLHQHKNCMLQMGGSDQWGNIVTGTELLRRKTGASAFALTTPLIKKADGTK
                     FGKTEGGNIWLNAARTSPYKFYQFWLNASDDDVKNYIRIFSIRNREEIEVLEKQHNEN
                     AAARILQKTLAQEITTRVHSEADYNSAVEASEILFGKAATESLKALPEATLLDVMEGV
                     PQTNVSHSSIAAGINTQILPSRSEAKKLIQAGGISINKEKVDDTTVTINVDRLLNNKY
                     LLIQKGKKNYYLVICED"
BASE COUNT          601 a          314 c          406 g          565 t
ORIGIN      
        1 ggcgtaatat gttgcacgat gttatgcccg gcaccgaaga gcaactcaat aaagaaatga
       61 caacgggtta cataggtttc gaccctacgg ccgatagcct tggtgtgggc aatcttgtgc
      121 aagtgatgac cttgttgcat tttcaaaggt gcggccataa gcctattgcg ttggtaggtg
      181 gtgcaacagg tatggtgggc gacccaagtg gcaaaagtgc cgaacgcaat ttactgagcg
      241 aagatatttt gaaccataat cttgacagcc agaaaaagca attggaaaaa tttctcaatt
      301 tcgattgtgg tgccaactct gccgagattg taaataacta cgattggttt aaggatttta
      361 cttttctcga ttttattcgt gatgtaggca aacacattac agtaaattat atgatggcaa
      421 aggatagcgt aaaatcgcgc ctcgatactg gtatgtcgtt tacagaattt agttaccagc
      481 ttgtgcaggg ttacgatttt gtgcacctgc atcaacataa aaattgcatg ttgcaaatgg
      541 gcggtagcga ccagtgggga aatattgtaa ccggcaccga attgcttcgc agaaaaacgg
      601 gggcttcggc atttgcactc actacaccat tgattaaaaa ggctgatggc accaagtttg
      661 gcaaaaccga aggtggcaat atttggctca atgctgcgcg aacatctcca tataaatttt
      721 atcagttttg gctcaacgcc agtgacgatg atgtaaaaaa ttatatccgc attttttcga
      781 ttcgtaaccg cgaagagatt gaagttttag aaaaacaaca taacgaaaat gctgccgcac
      841 gcatacttca aaaaacattg gcacaagaaa ttactacgag agtacatagc gaggccgatt
      901 ataatagtgc ggtggaggcg tcagaaatat tatttggcaa agcagctacc gaatcgctaa
      961 aagcattgcc cgaagccact ttgctcgatg taatggaagg cgtgccgcaa acaaatgtta
     1021 gccacagtag tattgctgcc ggaataaata cccaaatatt accatcgcgc agcgaagcaa
     1081 aaaaattgat acaagccgga ggcatcagca tcaacaaaga aaaagtggac gacacaactg
     1141 taaccataaa cgtagataga ttactcaaca ataaatatct gcttattcaa aaaggtaaaa
     1201 agaattatta tttggttata tgtgaagatt agtcatttgg ggatagtcat tgaggatggt
     1261 cattggtcat tgaggatggt cattgaggat tgtattttta attttcaatt aataattttt
     1321 aatttccgtg tcattggtca tttcaatact tttcatttgt aaattcttag tgggacatat
     1381 aattggaaat gaaattatcg atattttatt tgtgattgaa tttatttagc ctcaattcag
     1441 taacaccctt ctcgtaaaaa cgaaaaattc gttttgtatt aactcgccta aagttgaaaa
     1501 aagttatcta ctgaatgaat tgccttgagt cgttgttttc accggccaaa aatttcatca
     1561 acaacgtctt tcaggaggaa tcttattgtg taaacgaaaa tgtcgtttag aattaattta
     1621 tccaaaattg aaataaggtt cctcctgaat gacttgaggt ggttttgctt ttgaattaat
     1681 ttatctaaag tagaaataag tttcgtcctg aatgacttga ggtggttttg gctttgaatt
     1741 aatttatatg aagtagtgat aaggttcttc ctgaatgact tggggttgtg cacaaattta
     1801 tctaaagtag aaataagttt cgtcctgaat gactttgggt ggttgtgatg ttgaagtaac
     1861 ttagatataa tattgataca gataca
//
LOCUS       DAORQA010000338         1874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_13210_length_1874_cov_13.014294, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000338 DAORQA010000000
VERSION     DAORQA010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13210_length_1874_cov_13.014294"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /gene="glmS"
                     /locus_tag="PK736_09670"
     CDS             <1..1504
                     /gene="glmS"
                     /locus_tag="PK736_09670"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRC33695.1"
                     /translation="LLLKGHTFQSDTDTEVLIHLIEEIYDTENVELEHAVRIALLEVV
                     GAYAIVIISKKHNNQLIAARKGSPIVLGIGEGEYFIASDATPIVEYTKNVVYVDDNEM
                     VIVTDGELTIKTIQNELKTPFIHELELKMEAIEKGGYDHFMMKEIFEQPHSIWDSMRG
                     RFDAKTGRLRLGGVAQYEQKFMNAKRIIIAACGTSWHAGLVAEYLIEDLARIPVEVEY
                     ASEFRYRNPVIHEDDVVIAISQSGETADTLAAIELAKQKGATIFGVCNVVGSSIPRAT
                     HAGAYTHAGPEIGVASTKAFTAQVTVLTLMALQLAYKKGTITESKLREYLYELESIPK
                     KIEKVLLLNDSIKELAAKYKDARNFLYLGRGYGFPVALEGALKLKEISYIHAEGYPAA
                     EMKHGPIALIDEHMPVVVIATKNSSYEKVVSNIQEVKARKGNIIAIVTEGDDVVKQFA
                     DYVIEIPETDDILVPLVAVVPLQLLSYHIAVMRGCNVDQPRNLAKSVTVE"
BASE COUNT          597 a          356 c          421 g          500 t
ORIGIN      
        1 attgttgcta aaaggtcaca cttttcaaag cgatactgat accgaagtgc tgatacattt
       61 aatagaggaa atttacgaca ccgaaaatgt agaacttgaa catgcagtgc gtatagcatt
      121 gctcgaagtg gtaggggctt atgccattgt tatcatttcg aaaaaacaca acaaccaact
      181 tattgcagca cgtaaaggca gcccaatagt tttgggaatt ggcgaaggcg aatattttat
      241 tgccagcgat gccacaccta ttgtggagta cacaaaaaat gttgtgtatg ttgacgataa
      301 cgaaatggta atagttaccg atggtgaatt aaccataaag acaattcaga acgaattgaa
      361 aacccctttt attcacgagt tggaattgaa aatggaagcc atagaaaaag gtggttacga
      421 tcactttatg atgaaagaaa tttttgagca gccacattct atatgggaca gtatgcgtgg
      481 ccggttcgat gcaaaaacag ggcgtttgcg acttggtggt gtggctcagt acgagcaaaa
      541 atttatgaat gccaagcgta ttatcattgc agcatgtggc acatcgtggc atgcgggcct
      601 tgttgccgag tacttgattg aagacctggc acggattcct gttgaagtgg aatatgcttc
      661 ggagtttcgt tatcgcaatc ctgtaataca cgaagacgat gtggtgatag ccatttcgca
      721 gtcgggcgaa actgccgata cactggccgc catagaactt gcaaaacaaa aaggcgctac
      781 aatatttggt gtgtgcaatg tggtgggttc ttctattccg cgtgccacac atgcaggtgc
      841 gtatacacat gccggccccg aaattggtgt ggcatctacc aaggcattta ctgcacaagt
      901 tactgttctt acattaatgg ctttacaact cgcatataaa aaaggcacta ttaccgaatc
      961 gaaattgcgc gagtatttgt atgagttgga aagcattcca aagaaaatag aaaaagtttt
     1021 gttgctaaac gattcgataa aagaacttgc tgcaaaatat aaagatgcac gcaacttttt
     1081 atacctcggc aggggatatg gttttccggt cgcactcgaa ggcgcattaa aattaaaaga
     1141 aatatcgtac atacatgccg aaggttatcc tgcagccgaa atgaaacatg gaccaattgc
     1201 cctgatagat gaacatatgc ctgtggttgt tattgccacg aaaaattctt cgtacgaaaa
     1261 agtagtaagc aacatacagg aagtaaaagc gcgcaaagga aatattattg ctattgttac
     1321 cgagggcgat gatgtggtaa aacaatttgc cgattacgta atcgaaattc ccgaaacaga
     1381 tgatatactt gttccgctcg tggctgtagt tccattacaa ttactatcgt accacattgc
     1441 cgtaatgcgc ggttgcaatg ttgaccaacc tcgtaattta gccaaatcgg ttacggtgga
     1501 ataatttttt tcataagaca ttagaggtaa ggcaaaaggg gtaagtcaaa aggagtaagt
     1561 caaaaaaata tttggattta ttcggaattc attcgtgcat tcgcggcaaa aaaattacag
     1621 caatttcttt ttgtgcaaca cgtcattgcg agtgcaacga agcaatctct gcgacacagc
     1681 atcttgtcac cgctgagatt gcttcagtcg tacctccttc gcaatgacgg gaagaaagtg
     1741 aaattcattc gcgcattcgc ggcaaaaaaa attacagtaa tttctttaag cgatcacacg
     1801 tcattgcgag tggcgaaccc aaaacaggat aacaaataaa tgatgacatt tgctttcgca
     1861 taatatcatc gcag
//
LOCUS       DAORQA010000339         1836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_13494_length_1836_cov_7.455362, whole genome shotgun sequence.
ACCESSION   DAORQA010000339 DAORQA010000000
VERSION     DAORQA010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13494_length_1836_cov_7.455362"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PK736_09675"
     CDS             complement(<1..866)
                     /locus_tag="PK736_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC33696.1"
                     /translation="MWNNYFKTAWRNLLKSKGYSFINIMGLTMGLTCCFLILLFVKHE
                     MSYDKFQKNYDRIYRATYNPTFIDLPAPFAVLPPVAATLFKQSFPEMEEVARMYKRNV
                     SLSADGENGNKKYFEEEKFYFADPSILKIFTFDFVEGNPTSSLNNPFDVILTEDNAHK
                     YFGDKSAMGKTILFAGKYPMKVSGVVKSFPDNSHLKFNFIANYETMLATEAAAARKNF
                     EKNWVISHSYTYVLLKQNQSAANVNSRFAKFLLSNAPADYAKDIIYELQPMKNFHLHS
                     NLTEEPEPAGSIT"
     gene            complement(878..>1595)
                     /locus_tag="PK736_09680"
     CDS             complement(878..>1595)
                     /locus_tag="PK736_09680"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRC33697.1"
                     /translation="VNQAFVKEVGWKGSAIGKTIKELNGMEQNLKVVGVVRDYHFESL
                     HEKIIPQVFTCDKMVALQRFLIRIDEKNKSKTLAGIEQVYNQLLPYHPFKYEFIDDVN
                     KQYYKAEDKWKQIISMAAILTIFISCIGLFGLSSLAVQKRVKEIGVRKVLGASVMQIS
                     NLLTRNFISLIAIAFVIAVPAAWYAGNMWLQNYAYRMPFNASIFLLALVIILVVSLAT
                     ISFQSIKAAITNPVKSLRSE"
     assembly_gap    1596..1610
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1611..1827)
                     /locus_tag="PK736_09685"
     CDS             complement(<1611..1827)
                     /locus_tag="PK736_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33698.1"
                     /translation="MALLRQFQDKVAALPGVESVAPTMDGDWVTSGKVDGKNIEAKYD
                     FIGEEFLPTMKVPLTEGRNFSKEFPGDT"
BASE COUNT          549 a          370 c          310 g          592 t
ORIGIN      
        1 gttatactgc ctgcgggttc aggttcttca gttaagttag agtgtaaatg aaaatttttc
       61 atgggctgca attcatatat aatatccttt gcataatcgg ccggtgcatt cgaaagtaaa
      121 aattttgcaa agcgtgaatt tacatttgca gcactttgat tttgcttcaa caacacataa
      181 gtatatgagt gactgataac ccaatttttt tcgaaatttt ttcttgcagc tgcggcttcg
      241 gttgcaagca tggtttcgta attggcaatg aaattaaatt tcaggtgcga gttatcggga
      301 aacgatttta ctacacccga gactttcata ggatacttac cagcaaataa aattgtttta
      361 cccatagcgc ttttatcgcc aaaatattta tgtgcattgt cctcagtaag tataacatca
      421 aaaggattgt tgagcgaaga agtggggttg ccttcgacaa aatcgaaagt gaatattttt
      481 aaaattgatg ggtcggcaaa ataaaatttc tcttcttcaa aatatttctt gttgccattt
      541 tctccatctg ccgaaaggct aacattgcgc ttatacatgc gtgccacttc ttccatctcg
      601 ggaaaacttt gcttgaacaa tgtagccgca acaggtggca acactgcaaa cggtgctggc
      661 aaatcgataa acgttggatt gtaggttgca cgataaattc ggtcgtagtt cttctgaaat
      721 ttatcataac tcatttcatg ttttacaaaa agcaaaatga gaaagcaaca ggtaagcccc
      781 attgttaaac ccataatgtt tataaacgag taacctttgc ttttgagcaa atttcgccag
      841 gcagttttaa aataattatt ccacatcgta ttaattatta ttctgaacgc aaactcttaa
      901 caggatttgt aatggcagct tttatacttt gaaagcttat tgtagccaac gatacaacta
      961 aaataataac gagtgccaat aagaatattg aggcattaaa tggcatgcgg taagcgtaat
     1021 tttgcaacca catattgccg gcataccagg cggcaggcac tgcaataaca aatgcaattg
     1081 ctatcaatga aataaaatta cgcgttagta aattcgaaat ttgcatcacg cttgcaccta
     1141 acactttgcg tacgccaatt tcttttaccc gtttttgtac agccaacgag ctcaggccaa
     1201 acaacccaat gcacgaaata aaaatggtga gtatggccgc catcgaaata atctgtttcc
     1261 atttatcttc ggctttataa tattgtttgt ttacatcatc aataaactcg tatttaaatg
     1321 gatgataggg cagcaattga ttatacactt gctctatacc ggctaatgtt ttcgatttat
     1381 ttttttcgtc aatacgaatt aaaaaccttt gcagtgctac cattttatca catgtaaaaa
     1441 cctgtgggat tattttttca tggagcgatt cgaaatgata atcgcgcacc acacctacta
     1501 cttttaaatt ttgctccatg ccatttaact cttttatagt tttgccaata gcacttcctt
     1561 tccatcctac ttcttttaca aaagcttgat ttaccnnnnn nnnnnnnnnn tagtgtcacc
     1621 agggaattct ttcgaaaaat ttctgccttc agtcagcggt actttcatag ttggcaaaaa
     1681 ctcttcgcct ataaaatcat acttcgcctc aatatttttc ccatccactt ttccgcttgt
     1741 tacccaatcg ccatccatag taggtgctac actttcaact ccgggcaatg cggcaacttt
     1801 atcctgaaac tgtcttaata aagccatgtt atcgcc
//
LOCUS       DAORQA010000340         1835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_13499_length_1835_cov_10.929775, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000340 DAORQA010000000
VERSION     DAORQA010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13499_length_1835_cov_10.929775"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>419
                     /locus_tag="PK736_09690"
     CDS             <1..>419
                     /locus_tag="PK736_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33699.1"
                     /translation="GLVVGTYTVTVTDANGCTKTCVIELADPAELQAGAIPTDETCEY
                     LNNGMVEVQAIGGTGLFTVNIVGGVVNETVTGISFNYIFDSLAAGNYIITVTDANGCS
                     TQFSTDVFEPAPLAVTQTITQVACNGAATGAIDITVAG"
     assembly_gap    420..429
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <430..>1835
                     /locus_tag="PK736_09695"
     CDS             <430..>1835
                     /locus_tag="PK736_09695"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33700.1"
                     /translation="GTPSYTFAWSNSTTTEDQTNLSAGNYSVVVTDAAGCTTSASFVI
                     TEPTLLVATATSTNVTCNGANNGTLNASASGGVPTYTYSWSNSITGSSQTGLGSGTYT
                     VTVTDGNGCSKTSSTTIAEPAALAIILTPTNTTCALSNGSISASVSGGTVNYNYSWSN
                     GASMQNLSSIAAGTYTVNVTDANGCTNSGSITITNTGAIPAQPGAISGSSTICKNTSQ
                     SFSIIAVPGANSYTWSAGPGATITLGQGTTNVTIAFSNLATASSVSVFASNGCGNGLP
                     RTKAYTILNGTPATPTSILGPLWGNCRKVNVGYSCTLSAGATTYTWTVPAGVTINSGQ
                     GTNAIIVTYGLAFTGSGNISVTASNSCGTSTARTVVTNAKPQMLVISGLNYACQYQSG
                     LVYTVAAVPGAAGYTWTVVAGSTILSGQGTTSITVKWGVINGVIKCVANNACGSSIQA
                     QFPVSFVCKEDGTPFDLS"
BASE COUNT          532 a          373 c          406 g          514 t
ORIGIN      
        1 ggtttagtag tcggtacgta tactgttacg gttaccgatg caaatggatg tacaaaaacc
       61 tgcgttattg aattggccga cccggcagaa ctacaggcag gagcaatacc aaccgatgaa
      121 acttgtgaat acctcaataa tggtatggtt gaagtgcaag caataggagg aacaggatta
      181 tttacagtaa atattgttgg tggtgtagtg aatgaaactg ttaccggtat atcgtttaat
      241 tacattttcg atagccttgc agccggtaat tatattatta ctgttaccga tgccaatgga
      301 tgtagcacac agttttctac cgatgtattt gaacctgcac cacttgcagt tactcaaaca
      361 ataactcagg tagcttgtaa tggagcagca acaggtgcta tagatattac tgtcgcaggn
      421 nnnnnnnnnc ggtacgccat cttatacttt cgcatggagc aatagcacaa caacggaaga
      481 ccaaactaat ttgagtgcag gaaattattc tgtagttgtt accgatgcag caggttgtac
      541 tacatcggcc tcttttgtca ttacagaacc aaccctattg gtggcgacag caacatcaac
      601 aaatgtaacg tgtaatggtg caaacaatgg aacactcaat gcatctgcaa gtggaggtgt
      661 accaacatac acttattcat ggagtaatag cattacaggc agttcgcaaa ccggacttgg
      721 cagcggtaca tatactgtaa cagttaccga tggaaatggt tgcagcaaga cttcttcaac
      781 aacaattgca gagccagccg cgcttgctat tatacttaca ccaaccaaca caacctgtgc
      841 acttagtaat ggttcaataa gtgcaagtgt gtcaggtgga actgtgaact acaattacag
      901 ttggagcaat ggtgcctcaa tgcaaaattt atcttctatt gctgctggta cgtatacagt
      961 taatgttacc gatgcaaatg gatgtactaa ttctggaagt attaccatca ctaatacagg
     1021 ggcaatacct gcgcaacctg gtgctatttc aggttcctca accatatgta aaaatacttc
     1081 gcagtcgttt tctattattg cggtacctgg cgctaattca tatacatggt cggcagggcc
     1141 cggtgctaca ataacattag gccaaggtac taccaacgtt accattgcat tttccaattt
     1201 ggcaacagca tcttccgttt ctgtttttgc atcgaatggc tgtggaaatg gtttgccacg
     1261 aacaaaagca tacacaattt taaatggcac accggcaaca cctacttcaa tattaggacc
     1321 gctgtggggt aattgcagaa aagtaaatgt tggttactca tgtactttgt ctgcaggtgc
     1381 taccacatat acatggaccg tacctgctgg cgttactatc aattcgggtc agggaacaaa
     1441 tgccataatt gttacatacg gattagcgtt tacaggaagt ggtaacattt cggtaactgc
     1501 ctcgaacagt tgtggtacaa gtactgcacg taccgtggta acaaatgcta aaccacaaat
     1561 gcttgttatt tcaggattga attatgcctg tcaatatcaa tcgggcttag tttatactgt
     1621 ggccgcggtg ccgggtgctg ctggctatac ttggactgtg gttgcaggca gcacaatact
     1681 gagtggacaa ggtactacct ctatcactgt aaagtgggga gtaattaatg gtgttataaa
     1741 atgcgttgcc aacaatgctt gtggtagcag tatacaagct caattccctg tatcatttgt
     1801 ttgcaaagaa gatggtacgc ctttcgattt atccg
//
LOCUS       DAORQA010000341         1821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_13635_length_1821_cov_6.966025, whole genome shotgun sequence.
ACCESSION   DAORQA010000341 DAORQA010000000
VERSION     DAORQA010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13635_length_1821_cov_6.966025"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="PK736_09700"
     CDS             <1..96
                     /locus_tag="PK736_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRC33701.1"
                     /translation="QQCRSFEEATDNAVEALRKQITRHKEKVRGV"
     gene            complement(234..>1821)
                     /locus_tag="PK736_09705"
     CDS             complement(234..>1821)
                     /locus_tag="PK736_09705"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRC33702.1"
                     /translation="VQFPPGASNVVFTTDEVSNSYLTAYDVSNPNNIQYLDKIQSQNP
                     GSGSIIHNTYIKNEYAVCSYYRDGFIIVDASRPGNLVLTGWYDTSPSFAGNGFNGAWG
                     VYPYFPSGNVVVSDIENGLFVLTPNYVRACHIEGVVTDQSTSLAINGASVSISGPATL
                     AETSAANGQYKTGTSTAGTYTVTVSKAGYVTNVTSGVVLTNGILTTLNIALVPVGSCG
                     TPTGLSATSITQTTATLNWGAVSGAVSYNVQYRAVGAGTWTNGASASTSLAISGLVAS
                     TNYEFQVQAVCSGTSGTYSTLGTFTTLAPGGNCTSDIYETNNTSSTTKTIAVNTDIYA
                     LIGVAGDLDWFRFTTVSPNTKLKATLTQVPFDYDLRLYSNSNSLLTTSQNGGTTSETI
                     IRNATTAATYKLRVYGYNGAFSATSCYKLRVDVSSVNYVKTIPQDIETFKYTAENELN
                     AFPNPATDAVAFIFESDIETTGSISIFDLTGRMINNTQINVIEGQNMADLNIAYLANG
                     VYIVEVFDGTETIKVKVVKE"
BASE COUNT          544 a          365 c          354 g          558 t
ORIGIN      
        1 caacaatgtc gatcgtttga agaagctacc gacaatgctg tagaggcgtt gcgaaagcaa
       61 ataaccaggc ataaagaaaa agtacggggt gtataaatta aaaaaggctg tcacaaaaat
      121 gacagccttt ttatttggaa ccttatttta ttatttagtg atttattgaa tatttaaagg
      181 tatcaaaatc gcgttccaaa ctgattacag ttttcacaaa atcaactttg aatctactct
      241 tttacaacct tcacttttat agtttcagtt ccgtcaaaca cttcaactat gtaaactcca
      301 tttgcaaggt atgcgatgtt taagtcagcc atattttgtc cttcaatcac atttatttgc
      361 gtgttattta tcattctgcc cgttaagtca aatatcgaaa tgctgcctgt ggtttcaata
      421 tcgctttcaa atataaaagc aacagcatcg gtagcaggat ttggaaatgc atttaattcg
      481 ttttcggcag tgtacttgaa agtttcaata tcttgcggta ttgtttttac atagtttaca
      541 ctactcacat cgacccgcaa tttataacaa cttgtggcgc tgaatgcacc attgtaacca
      601 tataccctga gcttatatgt agcagctgtt gtcgcatttc gtattatagt ttcgcttgtt
      661 gtacctccat tttgcgatgt ggttaaaagt gaatttgaat tactgtacaa tcgcaaatca
      721 taatcgaaag gcacttgtgt taaagtggct tttaatttgg tatttggcga tacggttgta
      781 aatctaaacc aatcaagatc gcccgctact cctattaaag cataaatatc ggtattgaca
      841 gcaatggttt ttgttgtgga tgaagtgttg ttggtttcgt aaatatcgct tgtgcaattt
      901 ccacctggtg ccaaagtagt gaatgtgccc aaagtactat acgtacccga tgtaccgctg
      961 caaactgcct gtacctgaaa ctcgtaattg gtgctggcca ctagccctga tatggcaagg
     1021 cttgtagatg cagatgcacc gttagtccag gttcctgctc ctactgcacg atattgcaca
     1081 ttgtaagaaa cagctccact caccgcaccc cagttgaggg ttgcagtagt ttgtgttata
     1141 gaggtggcag acaaacctgt aggcgtaccg caacttccaa ctggaactaa tgcaatatta
     1201 agagtagtaa gaataccgtt ggttagcacc actcccgaag taacattagt aacataacct
     1261 gctttgctaa cagtaacagt atacgtacca gcagttgaag ttccggtttt atactggcca
     1321 tttgctgcag atgtttcggc aagtgtagca ggacccgaaa tgctaaccga tgcaccatta
     1381 atagccaaac ttgtagattg atctgtaact actccttcga tatggcaggc acgaacataa
     1441 tttggtgtaa gaacaaacaa tccattttca atatcgctta ccaccacatt tcctgaagga
     1501 aaataaggat atactcccca tgccccatta aagccattgc cggcaaatga tggagaagta
     1561 tcgtaccagc cggtgagtac aaggtttccg ggacgcgatg catcaactat aataaaacca
     1621 tcgcgataat atgaacatac agcatactca tttttaatat aagtattatg tataattgaa
     1681 cctgaccccg ggttttgcga ttgaatttta tcaaggtatt gaatgttatt tgggttcgat
     1741 acatcataag ctgttagata agagttactt acttcatcgg ttgtaaacac aacattgctg
     1801 gcaccaggtg gaaattgcac a
//
LOCUS       DAORQA010000342         1815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_13685_length_1815_cov_5.228409, whole genome shotgun sequence.
ACCESSION   DAORQA010000342 DAORQA010000000
VERSION     DAORQA010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13685_length_1815_cov_5.228409"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PK736_09710"
     CDS             <1..240
                     /locus_tag="PK736_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRC33703.1"
                     /translation="EITDLEDRIREFQQSAQENISKKKEEIYAPILKKAEEAIKGIAK
                     EKGYTYIFDAGVGVLLYSQDSDDILPLVKAKLGLK"
     gene            complement(329..904)
                     /gene="nadD"
                     /locus_tag="PK736_09715"
     CDS             complement(329..904)
                     /gene="nadD"
                     /locus_tag="PK736_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HRC33704.1"
                     /translation="MLIGLFFGSFNPVHNGHMIIAQHMCENTDMAEVWMVVSPHNPLK
                     PFGSLLQDYHRLQLVRIAIGDYKKIKASNIEFTLPKPSYTIHTLAYLKDRHPEHEFAL
                     IMGMDNLCTLNKWKNYEQILEQYSIYVYPRLGSDDCEFATHPKVRITTAPIMELSSTV
                     IRNSIKAKKDVRYMMPESVYNYIDEMNFYRK"
     gene            complement(924..1484)
                     /gene="gmk"
                     /locus_tag="PK736_09720"
     CDS             complement(924..1484)
                     /gene="gmk"
                     /locus_tag="PK736_09720"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRC33705.1"
                     /translation="MKLIVFCGPSGSGKTSIKNYLMQRIEVLSFSVSATTRAMRTNEH
                     NGVDYYFLTVEEFKNKINNHEFVEWEEVYTNGFYGTLKSEVERLASLNKVVIFDVDVE
                     GGLNIKKAYGHQCLALFVCPPSIEELRKRLTKRGTETDESLQKRITKAELELTYKNKF
                     DGVIVNENFETACDDSEKLVLQFIHN"
BASE COUNT          535 a          335 c          336 g          609 t
ORIGIN      
        1 gaaattaccg acctcgaaga tcgtatacgt gagtttcaac aatcggctca ggaaaatatc
       61 agcaaaaaga aagaagaaat ttacgcaccg attttgaaaa aagccgaaga agccataaaa
      121 ggaattgcaa aagaaaaagg ctacacttat atttttgatg ccggtgtggg cgtattactt
      181 tactcacaag acagcgatga tattcttcca ttagtaaaag caaaactcgg attgaaataa
      241 tttttctgaa tcgataatat aaaaaaagcg catcggttgg tgcgcttttt ttttggagtt
      301 tttttcggga tggaaagctc aaccatttct acttcctgta aaagttcatt tcatcaatat
      361 aattatacac gctttcgggc atcatgtacc gcacatcttt tttggctttt atagaattac
      421 gtatcactgt tgacgaaagt tccataatgg gtgcagtagt aattcttact ttgggatggg
      481 ttgcaaattc gcaatcgtca gagcctaaac gaggataaac atatatgctg tattgctcca
      541 gtatttgctc atagtttttc catttgttta aggtgcacag gttgtccatt cccattataa
      601 gcgcaaactc atgttcgggg tgcctgtctt ttaaatatgc cagggtgtga atagtataag
      661 atggttttgg taatgtgaat tcgatattgg aggcttttat ttttttgtaa tcgcctatgg
      721 caatgcgcac aagttgcagg cggtggtaat cttgcaatag tgagccaaac ggctttagtg
      781 gattgtgcgg acttactacc atccacactt cggccatgtc ggtattttca cacatgtgtt
      841 gtgcaataat catatgtccg ttatgtacgg ggttaaacga gccaaaaaac aaacctataa
      901 gcattgtacg tatactgtta tggttaatta tgtataaatt gcaaaactaa cttttcggaa
      961 tcgtcacagg cggtttcaaa attttcgttc actatcacgc catcaaattt gtttttgtac
     1021 gttaattcca attcggcttt agttattcgc ttttgcaggc tctcatcagt ttcggtacca
     1081 cgtttggtta atcgcttacg cagttcttca atcgaaggtg ggcaaacaaa taatgccaga
     1141 cattgatggc cataggcttt ttttatattc aatccgcctt ccacatcaac atcaaaaatc
     1201 actactttgt tcaatgaggc aagccgttca acttccgatt ttaatgtgcc ataaaatcca
     1261 ttggtataaa cttcttccca ctctacaaat tcgtggttgt ttattttgtt tttaaattcc
     1321 tcaaccgtta gaaagtaata gtctacacca ttgtgttcgt tggttcgcat agcacgtgta
     1381 gttgccgata ccgaaaagct caaaacttct atgcgttgca tcaagtaatt ttttatactc
     1441 gttttcccac tccccgatgg tccgcaaaac actattaact tcatacaact attttagatt
     1501 ttgtgagaaa aagtttccaa aacaattttt ggttaccaga ttgaaatact tttcatgccg
     1561 tcaaatatac taagagaagt tagattttcg aaccaaaata tatcttacac ctagtaatgc
     1621 cgaacaactt caatagcttg tttttgcaaa acctaagttg tttcgttata acgtggtttt
     1681 ttagcgaaac ataaaatgtt gctcaaagtg aaaatcgaga aaagatttac agaattttgg
     1741 aagtggtact atttcaattc tttgagcaaa ttggctgctt cgtctttttt gctttgttgg
     1801 aggttaacaa ctttt
//
LOCUS       DAORQA010000343         1815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_13686_length_1815_cov_4.770455, whole genome shotgun sequence.
ACCESSION   DAORQA010000343 DAORQA010000000
VERSION     DAORQA010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13686_length_1815_cov_4.770455"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PK736_09725"
     CDS             <1..616
                     /locus_tag="PK736_09725"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HRC33706.1"
                     /translation="TTDSLVEIFPGQGYLYVATTMKGIEGRTYTLTVNTEGKTVTATT
                     TLPKRIALDSLWFKVETNLDSLGFVWAHMKDPDTLGNAYRWFAKRITKDDNYIPPFGS
                     VFEDKFINGTEFDFAFPRGEMPNSANPDTTIERGYYKFGDTVITKFCTIDYPSFKFWR
                     SAENQAGSNGNPFAAPAFLLSNVIGGAGVFCGYSASYDTLIIKP"
     gene            1077..>1815
                     /locus_tag="PK736_09730"
     CDS             1077..>1815
                     /locus_tag="PK736_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33707.1"
                     /translation="MLMVLFVSQIEKGNAQDSINGKPSPILTFFGNGDMQKSLDEGEK
                     LPANTGLGVSYRQLYAPGVGKGLLWKFVDRLELEATINVASNVDTIIAAYDSTFVTNN
                     SEFGSSILTPLNSGQAIKIGLRLNFDRSRLVRLIDGVKAKYVGSNRNWMAMEGTTPKV
                     IKATNNCLRVGLFHEFIPKNLLDDYSINLAVNLAYNSVKGDVGLEQNGALRQSLMGSS
                     AKKFLGPEVSLEIRLRNLKAEFAYSWLQ"
BASE COUNT          539 a          335 c          359 g          582 t
ORIGIN      
        1 aacaaccgat tcattggtcg aaatatttcc cggacaggga tatttgtatg tagcaacaac
       61 catgaaaggt atagaaggcc gaacgtatac gcttacggtt aatactgaag gtaaaacagt
      121 tactgcaacc accacattgc ccaagaggat agctttggat agtttatggt ttaaagttga
      181 gactaatttg gatagcctgg gttttgtttg ggcacatatg aaagaccccg acacacttgg
      241 caatgcttat cgatggtttg caaaaagaat taccaaagat gataactaca ttccaccatt
      301 cgggtcggta tttgaagata aatttattaa cggtaccgaa ttcgattttg cttttccccg
      361 tggcgaaatg cccaatagtg caaatcccga taccactatc gaacgtggtt attataagtt
      421 tggtgataca gtaattacaa aattttgcac catcgattat ccatctttta aattttggcg
      481 cagtgccgaa aatcaagcag gaagcaacgg caatcctttt gcagcgcccg catttttatt
      541 gtcgaatgta attggcggtg ccggtgtatt ttgcggatac agtgcatcgt acgatacttt
      601 gatcataaaa ccataatgtt ggttttgcaa tcagccggct aaaaattgca aaacaattac
      661 ctatgtaaaa acggtggcgg catatatttt tcatacctaa aatctcagtt tgatttgctg
      721 caaaaatctg cagttttatt ttgtggcttt acgttatacc gtttgcttat tttgttctca
      781 tgcgtaaatc aaaaaaaaat aatttttatt ttttttattc aagttcaaat atgtttgcta
      841 ctttcatcgc gtctatttaa agcattacct ttatatcctt agcgttcaac atttaaagtt
      901 ctcgattttt tacttgtaat tgacttcaat gtttgataga aattatacct gaaaacgtat
      961 tctaaactta cgattttaat taacggagtt tgccgaaaat ccggtgaaga gtaggcgcat
     1021 cattttttac ttttatgcta acaaaattta cctcgcacta catcaaaact tttttgatgc
     1081 tcatggtgtt gttcgtttct caaattgaaa agggtaacgc acaggactcc atcaacggga
     1141 aacctagtcc cattcttacc ttctttggta atggcgatat gcagaagtcg ctcgatgagg
     1201 gcgaaaaact tcctgccaac actgggctcg gtgttagtta ccgccagttg tatgctcctg
     1261 gtgtagggaa gggattgcta tggaaatttg ttgatcgttt ggagttggaa gcaaccatta
     1321 acgttgcatc aaatgtagat acaattattg cagcttacga ttcaactttt gttaccaata
     1381 attcggaatt tggtagttct attcttactc cactcaattc agggcaggcc ataaaaattg
     1441 gcctgagact taattttgac cggtcacgtt tagtgcgttt gatagatggt gttaaagcga
     1501 aatatgtagg ctctaatcga aactggatgg caatggaagg cacaacacca aaagtaataa
     1561 aggctacaaa caactgcctt cgcgttggtt tattccacga atttattcct aaaaatttac
     1621 ttgatgatta ttccataaac ctggctgtga accttgctta caactcagta aaaggagatg
     1681 ttggtttaga acaaaacggt gcactgcgtc aaagtttaat gggctcttca gccaaaaagt
     1741 ttttgggacc cgaagtatcg cttgaaattc gattgcgaaa tttgaaagcc gaatttgctt
     1801 actcatggtt gcaat
//
LOCUS       DAORQA010000344         1787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_13944_length_1787_cov_5.218245, whole genome shotgun sequence.
ACCESSION   DAORQA010000344 DAORQA010000000
VERSION     DAORQA010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13944_length_1787_cov_5.218245"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>276
                     /locus_tag="PK736_09735"
     CDS             <1..>276
                     /locus_tag="PK736_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33708.1"
                     /translation="LASPFTIVAQKLVHPTGIFAVSDSIDAAIEDDIYTLDYVDGVVL
                     RYNWAILQPGKSTFNFAPVLSEISKAKFYGKKVSLGIVCGAGIPHWIY"
     assembly_gap    277..300
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <301..1341
                     /locus_tag="PK736_09740"
     CDS             <301..1341
                     /locus_tag="PK736_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33709.1"
                     /translation="KLDFKEIPYQGESDKVISFSMCAPWDKTYLKYFKDFIIQLNNAL
                     TADTSIYNNIVQVKVGGVNRYSLELALPSEVNLTNGKDTSTNATEIWQSAGYRPYFVL
                     DAFSQIVRHYRSVFKTKALNIPFIPDPYSLPLIGSAGQAIGKNKQTLMAQLIDKSVLI
                     AGNKLMVQWNALNKNFVPVALDSAMQNGAYGGLQISFKYYGNPPGDKSDSLMYLFDYA
                     FAKNINYMELHCGALRNLPEAAKYGYDLFHTRDGQITEDILVPHLYTNAPNPFVDFTY
                     IKLTAKAPRKPMMQLFDCNGIFIREVSPVKVDEYEYLYFISGENLPSGTYTVYVKVRE
                     KQLGMKILKINK"
     gene            complement(1345..>1787)
                     /locus_tag="PK736_09745"
     CDS             complement(1345..>1787)
                     /locus_tag="PK736_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC33710.1"
                     /translation="GHALNNLGDAYYKLGQIPQAEENFNKAIKYNPNYAQAWFNRANC
                     YGQTGKYQAALDDLNKAISLDQKYTDAYTKRGQAYSMLNQPEAAKADYAKSLQLKPNN
                     PEIYYNLGVMYINMNNKQEACVNFGKAASMNFDAAIKAYNEVCK"
BASE COUNT          503 a          340 c          353 g          567 t
ORIGIN      
        1 cttgcatcac cttttactat tgtggcgcaa aaattggtgc accctaccgg tatttttgct
       61 gtgagcgatt caattgacgc agcaatcgag gatgatattt atacccttga ttatgttgat
      121 ggtgtagtac taaggtataa ttgggctata cttcaacctg gtaagagcac ttttaatttt
      181 gctcctgtgc ttagcgaaat atctaaggct aaattttatg gcaaaaaagt ttcattggga
      241 atagtgtgtg gtgccggtat tccccattgg atatatnnnn nnnnnnnnnn nnnnnnnnnn
      301 aagcttgatt ttaaagagat tccatatcaa ggtgaaagcg acaaggtcat ttctttcagt
      361 atgtgcgctc catgggataa aacatatttg aaatatttta aagactttat catccaattg
      421 aacaatgcgc ttacagcaga tacaagcata tataataaca ttgttcaggt aaaagtggga
      481 ggtgtaaacc gttattcgct tgaactggca ttgcccagcg aagttaatct caccaatggc
      541 aaagatacat caaccaatgc aacagagata tggcaaagtg ccggctaccg tccatatttt
      601 gtgcttgatg ctttttcgca aattgtgaga cattaccgtt ccgttttcaa aacaaaagcg
      661 ctgaacattc cctttattcc cgatccatac agtttgcctt taattggaag tgccggacaa
      721 gccattggca aaaacaagca aacacttatg gcacagttga ttgataaatc tgttttaatt
      781 gccggcaata aactgatggt gcaatggaat gcattgaata aaaattttgt gcctgtagcc
      841 cttgattctg ccatgcaaaa cggagcatat ggcggattgc agatttcttt taaatattat
      901 ggaaatcctc caggtgataa gagcgattcg ttgatgtact tattcgatta tgcatttgcc
      961 aaaaacatta actacatgga attgcattgc ggtgctttgc gcaatctgcc cgaagcagca
     1021 aaatatggct acgacctttt tcatacccgc gatggccaga taaccgaaga catattagta
     1081 ccccatcttt acaccaatgc gccaaatcct tttgttgatt ttacatatat taagctaaca
     1141 gcaaaagcac cacgcaaacc tatgatgcaa cttttcgact gtaatggaat tttcattcgc
     1201 gaagtttctc ctgtaaaagt tgacgagtac gaatatctgt attttatatc gggcgagaat
     1261 ttgccttccg gtacgtatac ggtgtatgtt aaagttcgtg aaaaacaact gggaatgaaa
     1321 attcttaaaa tcaacaagtg atttttattt acatacttcg ttgtatgctt ttattgctgc
     1381 atcgaaattc atagatgcag ctttgccaaa atttacacag gcttcctgct tattattcat
     1441 gtttatgtac atcacgccaa ggttgtaata aatctcagga ttattcggtt ttagctgaag
     1501 cgattttgca taatctgcct ttgcggcttc gggctgattg agcatagagt atgcctgtcc
     1561 acgtttggta tatgcatcgg tatatttttg gtcgagcgat atggctttat ttaagtcgtc
     1621 aagtgcggcc tgatacttac ccgtttgtcc atagcagttt gctctattaa accaagcctg
     1681 cgcataattt ggattgtact ttatggcctt gttgaaattt tcttctgcct gcggaatttg
     1741 tcccagctta taataagcgt cacctaaatt attcagcgca tggccgg
//
LOCUS       DAORQA010000345         1780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_14006_length_1780_cov_3.744348, whole genome shotgun sequence.
ACCESSION   DAORQA010000345 DAORQA010000000
VERSION     DAORQA010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14006_length_1780_cov_3.744348"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /gene="purB"
                     /locus_tag="PK736_09750"
     CDS             complement(<1..1335)
                     /gene="purB"
                     /locus_tag="PK736_09750"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRC33711.1"
                     /translation="MDRLKSISPIDGRYFANCMELSPFFSEYALIRYRVLVEVEYFIA
                     LCKLPLPQLAGVQEKALLSLKAIAENFGDNDAIRIKEIERTTNHDVKAVEYFLREEFD
                     KINLGQYKEFIHFGLTSQDINNTALPMMMMHCMNYHYTTLLNNLLALLQSNALAWKNI
                     PMLAYTHGQPASPTLLGKEIYVFYERLRVQLLQLENIQYSAKFGGATGNFNAHMVAYP
                     HIDWHAFADNFVTTLGLSRSRHTTQIEHYDNIAAWCDNLKRINTILIDCCRDVWAYIG
                     MNYFVLEVKAGEVGSSAMPHKVNPIDFENAEGNLSFANALYEFFAAKLPISRLQRDLT
                     DSTVLRNIGVPVAHSFIAIKSIIRGLNKLTVNEKTLAHDLDNNWAVVAEAIQTILRRE
                     NYPNPYQKLKELTRGNLQIDEASIKDFISTLDVSDTVRNELQAITPHNYTGVF"
BASE COUNT          548 a          345 c          332 g          555 t
ORIGIN      
        1 gaatacaccg gtataattat ggggtgtgat agcttgcaat tcatttctta cggtatcgct
       61 tacatcgaga gtagaaataa aatctttgat ggatgcttca tctatttgca aattacctcg
      121 cgttagctct ttcaattttt gatatggatt gggatagttt tctctgcgca aaatcgtttg
      181 aatagcctcg gcaactacgg cccaattatt atcgagatcg tgggctaatg ttttttcatt
      241 tacagtgagt ttattcaacc cgcggataat tgattttata gcaataaagc tgtgagctac
      301 aggcacacca atatttcgca aaacggtgct gtctgtaagg tcgcgctgca agcgcgatat
      361 aggaagtttt gcagcgaaaa attcgtataa tgcattggca aaagaaaggt tgccctccgc
      421 attttcgaag tcgataggat ttactttatg tggcatggcc gatgacccta cttcgcccgc
      481 ttttacttcg agcacaaaat aattcatacc tatgtaggcc cacacatcgc gacagcaatc
      541 aatcaatata gtgttgatac gtttaagatt atcgcaccac gcggcaatat tatcgtaatg
      601 ttctatttga gtagtatgcc tcgaacgcga aagtcctaaa gtggtaacaa aattatcggc
      661 aaaggcatgc cagtctatgt gtgggtaggc caccatatgc gcattgaagt tgccggtagc
      721 accaccaaat tttgcactgt actgtatatt ttcaagttgt aataactgta cccgtaaacg
      781 ttcgtaaaaa acataaattt ccttaccaag caaggtgggc gaagccggtt gtccatgtgt
      841 ataggcaagc attggtatat ttttccatgc aagtgcattc gattgcaata gtgccaagag
      901 attattgagc aatgttgtgt aatgatagtt catgcagtgc atcatcatca tgggcagtgc
      961 agtattatta atgtcttggg aagtaagacc aaagtgtata aactctttat actgaccaag
     1021 gtttattttg tcaaattcct cacgcaaaaa atattctaca gcttttacat catggttggt
     1081 ggtacgctct atttctttta ttcggatggc atcattatcg ccaaaatttt cggcaatggc
     1141 tttcaacgac aacaaggctt tttcctgcac tcctgccaat tgaggcaaag gcaatttaca
     1201 caaggcaata aaatattcta cctccaccaa tacccggtag cgaataagtg catactccga
     1261 aaaaaatggc gacaactcca tacaatttgc aaagtaccgt ccatctattg gcgatatgct
     1321 tttcaatcta tccatgctta gaatttcatt aaaaaaagaa gtgcgaattt aatattttac
     1381 ttgttgtggc ggttaataca aaaaaaataa ttgggaagtg gttttattgt ggttagcatc
     1441 gttcatgaga aatgacaaga aaagctaaca atgttgaatt ccaaaccagc aatttagaaa
     1501 taaattaaac agcccttcag gtttcgacac tttgccaaaa aatcagtcta tttttcagtc
     1561 aggaaatctt gccaatccga aaataaattc tattattgtt tcgaatttat ttttggaatt
     1621 aaaactttgc aaaaaatatt tttccaagaa gtaattagat cactccttaa aacctcacat
     1681 tatgtaccgc atcaaaattt tgaaatatca acattgtagt tcatcattac tatgatgccg
     1741 gggcttttca ctattatttt cccccggcca aacctaatct
//
LOCUS       DAORQA010000346         1778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_14019_length_1778_cov_4.311085, whole genome shotgun sequence.
ACCESSION   DAORQA010000346 DAORQA010000000
VERSION     DAORQA010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14019_length_1778_cov_4.311085"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="PK736_09755"
     CDS             complement(<1..1448)
                     /locus_tag="PK736_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33712.1"
                     /translation="MIIDLSCTLNKANGLHSNNSGASITIAASQTLTIQNSRFDYNAG
                     SNTVYNLAVANFPSVYHTFYNSKVEATGVVGNSTTLQFLYNDSIKASINNKATGVIRV
                     SHYYNYMTGTFTNVAGSKITIDGANYTSDLYVQNGFTNKGTIELTSLTGYGSGLYIQN
                     GVLTNDTNATIDVIIGVGGARTLNGKITNNGSLKIGYELSSTSSSFINNAVGTVSGLY
                     NLTLTGTRFLNNGTITPGPITGVLGISGDAKNSATAIYNMQIGGLTPGTQHDQYNVTG
                     ADTLNGALNVSLINGYVPTLGDSFIVCNYGTRVGTFSSVTGNIPGGYSWNIFYRNNYA
                     VIRAGNPSNLTIVASAGPNGSISPSGNVSVVYNANQIFTITPNSNYVIDSVIVDGAYA
                     GKQSTYTFTNVTANHTIVAKFSIPGYTITASKTGNGTITPSGAVNVNNGGSQTFNFAP
                     ANTCSAIDSIIVDGSFIGTPASYTFNNVTANHT"
     assembly_gap    1519..1532
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1533..>1778)
                     /locus_tag="PK736_09760"
     CDS             complement(<1533..>1778)
                     /locus_tag="PK736_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33713.1"
                     /translation="LINNYGNLQLIYGNTIYANLKNKNTGTIRLSHYANTMHGKFSNE
                     AGSTLSLDGANYTTDITFDSAFVNNGNIDLRGVTGYG"
BASE COUNT          524 a          333 c          331 g          576 t
ORIGIN      
        1 gtatgatttg ctgtcacatt attgaaagtg taacttgcag gtgtaccgat aaaactaccg
       61 tcaacaatta tactatcgat ggcactacac gtattggcag gagcaaaatt aaatgtttgt
      121 gaaccaccat tatttacatt cacagctcca ctgggtgtaa tggtaccatt gccggtttta
      181 ctggcagtaa tggtataacc cggaatagaa aattttgcaa caatggtatg gtttgctgtc
      241 acattagtaa aggtataagt ggattgcttg ccagcatatg cgccatctac aataacagaa
      301 tcgataacat aattcgaatt tggtgtaatg gtaaatattt ggtttgcatt gtacaccacc
      361 gacacattgc ccgatggcga tatagatccg ttaggccctg cacttgcaac tatggtaagg
      421 ttagaaggat tgccggcacg tataactgcg tagttgtttc tataaaaaat attccatgaa
      481 tagcctcccg gtatatttcc agtcacactc gaaaatgttc caacacgagt accgtagttg
      541 caaacaataa aactatcgcc aagagtaggc acataaccat tgatgagcga aacattcaaa
      601 gcaccattta atgtatcggc accggtaacg ttatattgat cgtgttgggt gccgggggtt
      661 aagcctccta tttgcatatt gtaaattgca gttgctgaat tttttgcatc gccacttatt
      721 cctaatactc cggtaattgg ccctggagta atggttccat tatttaaaaa gcgtgtgcct
      781 gttaatgtca aattatacaa accacttaca gtgccaacgg cattattaat aaatgaggaa
      841 ctcgtgctgc tcaattcata tccaattttg agtgaaccat tgtttgttat ttttccgttc
      901 aaagttcgtg caccacctac tcctattatt acatctatag ttgcattggt atcatttgtt
      961 agcacaccat tttgaatata caatcccgag ccatatcctg taagtgacgt aagttctata
     1021 gtacccttgt ttgtaaatcc attctgaacg tataaatcgg aagtataatt tgcgccatca
     1081 atagtaattt tagagccggc aacatttgta aaagtacctg tcatataatt ataataatgc
     1141 gatacacgta taacacccgt tgctttatta tttattgatg ctttaatgct atcgttatat
     1201 aaaaattgta atgtggtact attaccaacc actccggtag cctctacttt cgaattataa
     1261 aaggtatgat ataccgaagg gaaatttgca acggcaaggt tataaactgt attactgcct
     1321 gcattatagt caaatcttga attttgtatt gttagtgttt ggcttgcggc tattgttata
     1381 ctcgcacctg agttattgct atgcaatccg ttggctttat taagagtgca acttaaatcg
     1441 atgatcatag tacctttatt attgatgctt gcagtaatgt aatgcggccc gtattcgaaa
     1501 gaatgtattg tgccagtgnn nnnnnnnnnn nnaaccataa cctgtaacgc cccgtaaatc
     1561 tatattgcca ttatttacaa aggcactgtc gaatgttata tcggttgtat agttagcacc
     1621 atcgagcgac aatgtgctgc cggcctcgtt gctaaattta ccatgcattg tattagcata
     1681 atgcgataga cgaatagttc cggtgttttt atttttcaaa tttgcataaa tggtattgcc
     1741 ataaatcaat tgaagatttc cataattatt tatcaaag
//
LOCUS       DAORQA010000347         1777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_14027_length_1777_cov_6.234611, whole genome shotgun sequence.
ACCESSION   DAORQA010000347 DAORQA010000000
VERSION     DAORQA010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14027_length_1777_cov_6.234611"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PK736_09765"
     CDS             <1..714
                     /locus_tag="PK736_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRC33714.1"
                     /translation="GMNVAMNKTGYESFDKVFNQGMKALSVDEFETVVETAGALILDT
                     RTNKDFAKGFIPQSINIGIDGDFAPWVGALIADVKQPIVLITNQGSEEESVTRLSRVG
                     FDNILGHLKGGFEAWKNNGEEVDSVNRITAEQFANEVKIGESKIIDVRKESEYAAEHI
                     EDAYSKPLAYINDWVKDINPDEHFFMHCAGGYRSMIAASILQARGYRNFTEVEGGFNA
                     ISKTSLPKTDFICQSKILK"
     gene            766..1080
                     /locus_tag="PK736_09770"
     CDS             766..1080
                     /locus_tag="PK736_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRC33715.1"
                     /translation="MGFFSNLFGGGSNNDVLSTMVNNGAYLVDVRSESEFAQGSVKGA
                     VNIPLDKLTNQLAKFKNKEQVVVFCRSGMRSEQAKSILNQYGITNVTNGGTWQNVAKL
                     LS"
     gene            1083..1319
                     /locus_tag="PK736_09775"
     CDS             1083..1319
                     /locus_tag="PK736_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33716.1"
                     /translation="MKASTIFIKWNFMRVLQFILGSIVLIQALIERDFLFGVGGLLFI
                     LMAIFNLGCCNNACYTPTKKNKTPQDVVIYEEVV"
     gene            1303..>1498
                     /locus_tag="PK736_09780"
     CDS             1303..>1498
                     /locus_tag="PK736_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HRC33717.1"
                     /translation="MKKWFSDQLLYIIGAIVGAIAGYFYWQQVGCDSGTCMITSKPIN
                     STLYGALLGALLFSMFKHEEI"
     assembly_gap    1499..1529
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1560..>1777
                     /gene="trxA"
                     /locus_tag="PK736_09785"
     CDS             1560..>1777
                     /gene="trxA"
                     /locus_tag="PK736_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRC33718.1"
                     /translation="MTFNQIIQSDKPVLIDFFAEWCGPCKTMAPILKQVKDTIGDTAK
                     IIKIDVDKNQQTASNYQIQGVPTLMLFK"
BASE COUNT          593 a          277 c          364 g          512 t
ORIGIN      
        1 ggaatgaacg tagcaatgaa taaaaccggc tacgaaagtt tcgataaagt ttttaatcag
       61 ggtatgaaag ccttgtcggt tgatgaattc gaaacggtag tggaaactgc tggcgcatta
      121 attcttgata cacgaaccaa taaagatttt gccaaaggtt ttattcctca atctataaac
      181 attggtatcg atggagactt tgctccgtgg gttggtgctt tgatagccga tgtgaagcag
      241 cctattgttt taataacaaa tcaaggcagc gaagaagaat ctgtaacacg attaagccgt
      301 gtgggtttcg ataatattct cggccatttg aaaggtggct tcgaagcatg gaaaaataat
      361 ggcgaagaag tggacagcgt aaatcgaatt actgcggagc agtttgccaa cgaagtaaaa
      421 attggcgaga gcaaaattat agatgttaga aaagaaagtg agtatgctgc cgaacatatt
      481 gaagatgctt atagcaaacc attagcttac atcaatgatt gggtgaaaga tataaatccg
      541 gatgaacatt tttttatgca ttgtgccgga ggatacagaa gcatgattgc agcatccatt
      601 ttgcaagctc gtggatatcg aaattttacc gaagtggagg gagggtttaa tgcaatttca
      661 aaaacatcac ttcctaaaac cgattttatt tgtcaatcga aaatattaaa ataaaaagta
      721 ctgatacatt agttataatt tcaacttgta atacatacaa acaacatggg attttttagt
      781 aatttatttg gtggtggcag caataatgat gtgttgtcaa caatggttaa taatggcgca
      841 tatttagtag acgtgcgcag cgagagcgag tttgctcaag gtagtgtaaa aggtgcggta
      901 aatattcctt tggataaatt gactaatcaa ctagccaagt ttaaaaacaa agaacaagta
      961 gttgtttttt gccgaagtgg tatgcgtagt gagcaggcaa aatctatttt aaatcaatat
     1021 ggaattacca atgtcactaa tggtggcaca tggcaaaacg tggcaaaatt actatcttaa
     1081 aaatgaaagc ttcaaccata tttatcaagt ggaattttat gcgtgtgctg caattcattc
     1141 ttgggtctat cgtcttaatt caagcactca tcgaacgcga ttttctgttc ggtgttggcg
     1201 ggcttttatt tattcttatg gcaattttca atttgggctg ctgtaataat gcatgttata
     1261 ctccaacaaa aaaaaataaa acacctcaag atgttgttat ctatgaagaa gtggtttagt
     1321 gatcaactgc tgtatataat tggtgctatc gttggcgcta ttgccggata tttttattgg
     1381 cagcaagttg gttgcgattc gggcacgtgt atgattacat cgaagccaat taacagcaca
     1441 ctatatggtg ccttattagg tgctctgtta tttagcatgt ttaaacacga agaaataann
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aacataaaac aaaataatac tgaacagcaa
     1561 tgactttcaa ccaaattata caatcggaca aaccggtact catcgatttt tttgccgaat
     1621 ggtgtggccc atgcaaaacc atggcaccca ttttaaaaca agtaaaagac accataggtg
     1681 atacagcaaa aattataaaa attgatgtcg ataaaaatca acaaaccgca agcaattatc
     1741 aaatacaggg agtacctacg ctcatgctat ttaaaaa
//
LOCUS       DAORQA010000348         1762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_14166_length_1762_cov_6.641476, whole genome shotgun sequence.
ACCESSION   DAORQA010000348 DAORQA010000000
VERSION     DAORQA010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14166_length_1762_cov_6.641476"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1461)
                     /locus_tag="PK736_09790"
     CDS             complement(4..1461)
                     /locus_tag="PK736_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRC33719.1"
                     /translation="MKILVTHSYFLRFDAKQWKQQQPYAPLATLYAVSVLENLNHDVV
                     FTDMMFAKSADEIIPHLQRHKPDIVLIYDDGFNYLTKMCLTNMREACFSMINYSKQSQ
                     CKVIVSSSDSTDHSDEYLKHGADFILVGEGETTLKLLVEKIENNDLDFTEVAGLRFLN
                     DGKMLATPPRDVIRELDSIPLPAWDKIDMQSYKHAWQKKWGYFSINMTTTRGCPFKCN
                     WCAKPIYGNRYNVRSAALVVDELVMLKKKLNYNHVWFCDDIFGLKPGWIKQFAEAIKS
                     RNLKIKFKIQCRADLLLEENNIQWLAQAGCDEVWMGAESGSQKILDAMDKGTKIEQIE
                     LATALLKKHGIKPCFFLQFGYPGEIKEDILLTLNMVNKLQPFDIGVSVSYPLPGTKFY
                     ETVKSQLSQKSNWSDSDELALMFKNTYPSSFYKELHRYLHRKFRGRQGVSAWQKIASF
                     KRINLGQLKSAVLMPYHFVQQFYHQIQLQKFDFKV"
     gene            complement(1458..>1762)
                     /locus_tag="PK736_09795"
     CDS             complement(1458..>1762)
                     /locus_tag="PK736_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33720.1"
                     /translation="EKVNEVRPAKFLLTIKNGFEFVLKQNVFNGLENFCKKQTTSFWQ
                     RKFKNEGPAWFKNQLQNTAQISKHHPNGYRNKVLEMYEMRMAKFSKNDSIPEGSSE"
BASE COUNT          513 a          352 c          286 g          611 t
ORIGIN      
        1 atactaaact ttaaaatcga atttttgcaa ttgaatctga tgataaaatt gttgcacaaa
       61 atggtatggc attaacactg cgctctttag ttgccctaag tttattcttt taaatgaagc
      121 tattttttgc caggcgctca caccctgccg tccacgaaat tttcgatgta aatacctgtg
      181 aagttcttta taaaaagatg atgggtaagt atttttaaac atcaatgcaa gttcatctga
      241 atcggaccag tttgattttt gcgataattg agatttcact gtttcataaa atttagtgcc
      301 cggtagcgga taacttaccg atacgccaat atcaaaaggc tgcagcttgt tcaccatgtt
      361 taatgtcaac aaaatatctt cttttatttc gcccggataa ccaaactgta aaaagaagca
      421 aggttttatt ccatgctttt ttaataaggc agttgccaat tctatttgtt caattttcgt
      481 tcccttatcc atggcatcca atattttttg tgaaccactt tcggcaccca tccatacttc
      541 atcgcaacct gcctgtgcca gccattgtat attattttct tcgagaagca aatcggcccg
      601 gcattgaatt ttaaatttga tttttaaatt gcggcttttt attgcttcgg caaattgctt
      661 tatccatccg ggttttaaac caaaaatatc gtcacaaaac catacatgat tgtagtttaa
      721 tttcttcttg agcattacca attcatcaac aaccaatgca gcactacgaa cgttataacg
      781 gttaccataa ataggtttgg cgcaccagtt acatttaaat ggacaccctc gggtggtggt
      841 catattaatt gaaaaatatc cccatttctt ttgccatgca tgtttatatg actgcatatc
      901 aattttatcc caagccggaa gtggaattga atccagttct ctaattacat cgcgaggtgg
      961 tgtggctaac attttgccat cgttcaaaaa ccgaagacca gccacctcag taaaatctaa
     1021 atcgttattt tcaatttttt caaccaataa cttcaatgtt gtttcgcctt cgcccactaa
     1081 aataaaatcg gcaccatgtt tcaaatattc gtcactatga tcggttgagt cgctgctact
     1141 tactattact ttacattgcg actgctttga ataattaatc atcgaaaaac atgcttcacg
     1201 catattggtt aggcacatct tggtaaggta attaaagcca tcatcgtata tcaatacaat
     1261 gtcaggttta tgcctttgta agtgcggtat aatctcatcg gcacttttgg caaacatcat
     1321 atcagtaaaa actacatcat gatttaaatt ttccaaaacg cttactgcat ataatgttgc
     1381 aagcggagca taaggctgtt gctgcttcca ttgtttggca tcgaaacgta aaaagtatga
     1441 gtgagttact aatattttca ttctgaactt ccttctggaa ttgaatcgtt tttagaaaat
     1501 tttgccatgc gcatttcata catttccaac actttgttgc ggtatccatt tggatgatgt
     1561 ttcgaaattt gcgcggtatt ttgcaattga tttttgaacc atgcaggacc ttcgttctta
     1621 aatttccgct gccaaaagga tgttgtttgc tttttacaaa aattttcgag tccattaaat
     1681 acattttgct tcagcacaaa ttcaaagcca tttttaatgg tcaataaaaa tttggcaggt
     1741 cgcacttcgt ttactttttc ac
//
LOCUS       DAORQA010000349         1758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_14199_length_1758_cov_6.901938, whole genome shotgun sequence.
ACCESSION   DAORQA010000349 DAORQA010000000
VERSION     DAORQA010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14199_length_1758_cov_6.901938"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..1642)
                     /locus_tag="PK736_09800"
     CDS             complement(230..1642)
                     /locus_tag="PK736_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRC33721.1"
                     /translation="MQKITLIIVLFLNTAFISNAQIKTYYYPEDTLVAKNLEDWKKLK
                     FGLLMHWGTYTQWGIVESWSICSEDEGWCERRGPYANDYTEYKKQYEGLQKTFNPINF
                     APDKWATAAQYAGMKYVVFTTKHHDGFCMFDTKYTDYKITSDNCPFAKNSKSNVTKEV
                     FDSFRNKGFKVGAYFSKPDWHCPDYWWPRFATPNRCSNYDTKKYPQKWQAFEDFTYNQ
                     LNELTTQYGKVDLLWLDGGQVRPDSTVTEEERPWIKVPYTQGINMHRIANMARANQPG
                     ILMVDRSVYGYYQNYLTPEQQIPDKPLDYPWETCLTMAGSWSHVPGDKYKTTNNLIHT
                     LVDIVAKGGNLLLNIGPDASGNWDDTAYTRLQEIGNWMKINSSCIYNSTAVAPYKSGR
                     FCFTQVGKTIYAIYLLNEGETTATTLNISNYIPSSKSKLTLLGLKTKLNFKTENGNTI
                     IEVPPLDKNIFAHALVFKFE"
BASE COUNT          464 a          314 c          371 g          609 t
ORIGIN      
        1 ggattatagg cagtgattgt ttataaaaaa taatttttag aacatgttta tttaggattt
       61 atgtaacccc acccacaaag acggaaacca ttgcccatag ccccgattgc agcggttagc
      121 gtatgccatg caaataaaaa acaatatgat atggcataac tatgagcgga aagcgggacc
      181 gcacctttgg cagaatgata aaccgttgtg ctacaaaaaa agtattatgt tattcgaatt
      241 tgaaaacgag tgcgtgtgca aaaatgtttt tatcgagcgg aggtacttca attattgtat
      301 ttccattttc ggttttgaag ttgagttttg tttttaatcc caacagcgtg agtttcgatt
      361 ttgatgatgg aatgtaattg cttatgttaa gtgtggtagc agttgtttcg ccttcgttca
      421 ataaatatat ggcgtaaatt gttttaccaa cttgtgtaaa acaaaaccgg ccgcttttgt
      481 atggtgcaac tgcggtgctg ttgtatatgc atgaggaatt tattttcatc caattgccaa
      541 tttcttgcag gcgtgtatac gcagtgtcgt cccaatttcc gctagcatcg gggcctatgt
      601 tgagtaaaag atttccgcct ttggccacaa tatcgaccaa tgtatgaatg agattgttgg
      661 tggttttgta tttgtcaccg ggcacatgac tccagctgcc ggccatggtc aggcacgttt
      721 cccagggata atcgagtggc ttgtccggaa tttgttgttc gggagttaag tagttttggt
      781 agtagccgta tacgctgcga tccaccatta aaataccagg ttgatttgca cgtgccatgt
      841 ttgcaattcg atgcatattt attccctgtg tgtaaggcac ttttatccag gggcgttctt
      901 cttccgtcac tgtactatcg gggcgcactt gcccgccatc gagccacagc aaatcgacct
      961 tgccatattg tgtggtgagt tcgttaagtt gattgtaagt aaagtcttca aatgcctgcc
     1021 acttttgagg atattttttt gtatcgtaat tgctgcatct gttcggtgtt gcaaaacgtg
     1081 gccaccagta atcggggcaa tgccaatcgg gtttactaaa atatgcacct actttaaaac
     1141 ctttgtttcg aaaagaatca aaaacttctt tggtcacatt cgatttgctg ttttttgcaa
     1201 atgggcaatt atcggaagtg attttataat cggtatattt tgtatcgaac atgcaaaaac
     1261 catcgtgatg cttggtagta aacaccacat atttcattcc ggcatattgt gctgcagtag
     1321 cccatttatc gggtgcaaaa tttatggggt tgaatgtttt ttgcaatccc tcgtattgct
     1381 ttttatattc tgtataatcg ttggcatatg gccctctgcg ttcgcaccag ccttcgtctt
     1441 cgctgcatat actccaactt tcgacaatgc cccattgggt gtatgtgccc cagtgcatga
     1501 gcaaaccaaa ttttaatttt ttccagtctt ctaaattttt tgcgactaaa gtatcttcag
     1561 gataatagta tgtttttatt tgtgcattgc ttataaatgc tgtatttaaa aagagaacga
     1621 ttatgagtgt tattttttgc attttatatt tttttaagaa tatgaaatta aattttgttg
     1681 ttagaaaatg ttgtgagcaa aatatatttt atgttttgaa ttttaattgt gcattgagag
     1741 atataccaga acctgaaa
//
LOCUS       DAORQA010000350         1757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_14206_length_1757_cov_11.135723, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000350 DAORQA010000000
VERSION     DAORQA010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14206_length_1757_cov_11.135723"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..457
                     /locus_tag="PK736_09805"
     CDS             68..457
                     /locus_tag="PK736_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRC33722.1"
                     /translation="MYSEDFFSQWLGIKVVAIRQGYCLLQLTIVKQMLNGFGIAHGGI
                     SYSLADSALAFASNSYGIQSVSIETSISHTAPLKEGDVINAEATEVSRTNKIAIYNVH
                     VTNQLNKTVALFKGTVYITHKEWFPKE"
     gene            complement(647..>1757)
                     /locus_tag="PK736_09810"
     CDS             complement(647..>1757)
                     /locus_tag="PK736_09810"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33723.1"
                     /translation="GGDSTNLFGGGYFPTSLLYSVVDMTLDNNKGDVTLKNQIIITDT
                     LGGAINAVKHGNGRDWWVFNFRYNSNTIYGLLITPNGIQGPYTQNFGDSIIGAVGQGC
                     FSPDGSQFARYNKYFPYNDLDVYDFDRCTGLFSNWRNVKINDSTSAGGVAFSPNGIYI
                     YVSSLTKVYQFCTDSLNLANSMDTVAIYDGFVDPITPLPTTFFLAHLAPDAKIYFNCG
                     YSVTYMHVINNPDTVAKFCGMVQHGIKVPTFNAWTMPNLPNYHLGPKYGSVCDSLSTD
                     LTPNPSPFGEGNSLRINPNPSAGNFYVNYTLPPNTNGVLHIYDLVGNEVMQKNVYWYF
                     GYLQIDATTLQNGMYIASLEIGGRHWAGGKIVISK"
BASE COUNT          581 a          347 c          331 g          498 t
ORIGIN      
        1 ccataaaatc aagagtaaga ttaatatgag taaagaacac aatattccgc aaagggttgt
       61 agagaaaatg tacagcgaag atttttttag ccaatggctg ggaataaagg tggtagccat
      121 tcgccagggg tattgcttac ttcaacttac cattgtaaaa caaatgctca atggatttgg
      181 tattgctcat ggaggaattt cttatagttt ggccgacagc gctttagctt ttgccagcaa
      241 ctcgtacggt attcaaagtg tttctataga gacttctatt tcgcatactg caccattaaa
      301 agaaggcgat gtgataaatg ccgaagcaac cgaagtaagc aggactaata aaattgctat
      361 ttacaatgtg catgttacca atcaattgaa taaaaccgtg gccctgttta aaggaactgt
      421 ttacattaca cacaaagagt ggtttccaaa ggagtaaaag tttttcttgc tttgaaaaaa
      481 ggcaattctc ttaaaaccat caataacttc ttgcgtttaa agtttagaga aaattacaac
      541 cttactcaaa atgccgtgcc ccagtaacaa gcagagattt ctacttccaa tgacaatcgg
      601 aatggcattg acgataatga caatggcaag aatgcctcaa tgacaattat ttactaataa
      661 ctatttttcc cccagcccaa tgtctgccac ctatttctaa agaggcaatg tacattccgt
      721 tttgtaaggt ggtggcatct atttgtaaat agccaaaata ccagtacaca tttttttgca
      781 tcacctcgtt accaaccaaa tcataaatat gcaaaacacc atttgtattg ggcggcaatg
      841 tatagttcac ataaaaattg cccgcacttg ggttaggatt tatgcgcaag ctattcccct
      901 ctccaaaggg agaggggtta ggggtgaggt ccgttgacaa actatcgcat acactgccat
      961 actttggccc aaggtggtag ttgggtaggt tgggcattgt ccaggcatta aatgttggaa
     1021 ccttaatgcc gtgttgaacc ataccacaaa atttagcaac agtatccgga ttatttatta
     1081 catgcatata tgtaacacta taaccgcaat tgaaatatat tttagcatcg ggcgctaaat
     1141 gcgccaaaaa gaatgtggtt ggcaaaggtg taatggggtc aacaaaacca tcgtaaattg
     1201 caactgtatc cattgaattt gctaaattta aagagtcagt acaaaactga tacacctttg
     1261 tcagggaaga tacataaata taaataccat ttggcgaaaa cgccacaccc ccagccgaag
     1321 ttgaatcgtt tattttaaca tttcgccaat tactaaatag cccagtgcat ctatcaaaat
     1381 cgtaaacatc caaatcgtta taagggaaat atttgttgta acgagcaaat tgactaccat
     1441 cgggcgaaaa gcaaccttgg cctacggcac caataataga atcgccaaaa ttttgtgtgt
     1501 atggcccttg aatgccgttt ggggttatga gcaaaccata tatggtattt gaattgtacc
     1561 taaaattaaa aacccaccaa tcgcgaccat tgccgtgctt tactgcattg atggcacctc
     1621 ctaaagtatc ggtaataatg atttggtttt ttaacgtaac atcccctttg ttgttatcca
     1681 atgtcatatc gacaacacta tatagcaaag aagttggaaa ataacctcca ccaaataaat
     1741 ttgtagaatc accgccc
//
LOCUS       DAORQA010000351         1756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_14226_length_1756_cov_5.883010, whole genome shotgun sequence.
ACCESSION   DAORQA010000351 DAORQA010000000
VERSION     DAORQA010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14226_length_1756_cov_5.883010"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PK736_09815"
     CDS             complement(<1..847)
                     /locus_tag="PK736_09815"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRC33724.1"
                     /translation="MAQQIKVFAPATVANLGSGFDLLGLAVYGAGDEIIVTENNLNKL
                     FIEKITGDENKLPLEAIENTCTVAMQAMLDELKIKTGFNVTINKQMPLGSGMGSSAAS
                     AVGGVFALNELLNKPFTKAELIKFALEGEYIASRGYHADNIAPCMLGGITFISSTQPL
                     KVHALPCPDNFYITLLHQPVQILTAEARKILPPHYTRDIVIKQNAAIASFVNGLHQND
                     FESISFGLQDFIAEPYRKNLIPHYSELKNIAMQTGAVAITTTGAKLFFASFNILVAAI
                     ATAVWG"
     gene            complement(847..>1756)
                     /locus_tag="PK736_09820"
     CDS             complement(847..>1756)
                     /locus_tag="PK736_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HRC33725.1"
                     /translation="IKVQNAKQMLQDSGEAMNMSKYVKRMLELNLANSVFVDCTANDT
                     VAKHYEKILTSAISIVTPNKVANSGSYADYKLLKDTADKHHVKYLYETNVGAGLPVIN
                     TLKDLIISGDRIIRIDAVLSGTLSFIFNTFNSNLKFSDVVMQAKEKGFTEPDPRDDLS
                     CKDFARKLLILARESGTQIELDEIVIHDWLPAECMKPKDVAGFFEVLKKYDDHFSKLA
                     LKAEKENKVLRMIGSYIHGKAEMKLIAVDATNPFYNLSGSDNLIAFTTQRYHSQPLVI
                     KGPGAGAEVTAAGVLAEVISISNYLL"
BASE COUNT          492 a          367 c          319 g          578 t
ORIGIN      
        1 atccccatac tgctgtagca attgcagcaa caagaatatt gaacgaagcg aaaaataatt
       61 tagcaccggt agttgttatg gccactgcac ctgtttgcat agcaatattt tttagttcac
      121 tgtaatgcgg aatcaaattc tttcgatatg gttcggcaat aaaatcctgc aaaccgaaac
      181 ttatactttc aaaatcgttc tggtgcagtc catttacaaa acttgcaatg gcagcatttt
      241 gttttattac aatatcgcgg gtatagtgtg gtggtaaaat ttttcgcgct tctgctgtta
      301 atatttgtac aggttgatgc aacaaggtga tataaaaatt atcgggacaa ggcaatgcat
      361 gcactttcag cggttgtgtg ctacttataa aagtgatgcc gcccaacatg catggtgcta
      421 tgttatctgc atgatatcct cggctggcaa tgtattcgcc ttcgagggca aacttaatta
      481 actctgcttt tgtaaacggt ttgttcaaca attcattcaa tgcaaacact ccaccaaccg
      541 cacttgcagc actgctcccc attccgctac cgagtggcat ttgtttattg atagtaacat
      601 tgaatcctgt ttttattttc agttcatcga gcatagcttg catggcaacc gtacacgtat
      661 tttcgattgc ttcaagcgga agtttatttt catcgcccgt aattttttca ataaataatt
      721 tgttgaggtt gttttcggtt acaattattt cgtcaccggc accatacacg gccaaaccta
      781 gaaggtcgaa accacttcct aaatttgcga ctgttgccgg agcaaaaact tttatctgtt
      841 gcgccattac aataaataat tactgatgct tattacttcg gcaagcacac ctgctgcagt
      901 aacttcagcc ccggctcccg gccctttaat taccaatggt tgcgaatgat atcgttgtgt
      961 ggtaaatgct ataaggttat cgctaccgct gagattgtaa aatggattgg tggcatctac
     1021 agcaataagt ttcatctccg ctttgccatg gatatacgaa cctatcatgc gcaaaacttt
     1081 attttctttt tcagctttca aagcaagctt cgaaaaatga tcgtcatact ttttaagcac
     1141 ttcaaaaaat ccggccacat cttttggttt catacattct gccggcaacc aatcgtgaat
     1201 tactatttca tccaactcaa tttgagtacc cgattcgcga gcgagtatta aaagtttgcg
     1261 ggcaaaatct ttacacgata aatcgtcacg gggatcgggc tcagtaaatc ctttttcttt
     1321 tgcctgcatt accacatcac taaatttcag attgctattg aacgtattaa aaataaatga
     1381 tagagtacca gataacacag catcgatgcg gataattcta tcgccactta taattaaatc
     1441 tttaagggta ttaattactg gcaaacctgc ccctacatta gtttcgtata aatattttac
     1501 atgatgttta tcggctgtgt ctttcaataa tttgtaatct gcatacgagc ctgagttggc
     1561 aactttgtta ggcgtaacaa tactgatggc actggttaaa attttttcgt aatgcttagc
     1621 aactgtatcg tttgcagtgc agtcaacaaa aacactgttt gctaaattta gttcgagcat
     1681 gcgctttaca tacttgctca tattcattgc ttcgccacta tcttgtaaca tttgtttggc
     1741 attttgaact ttgata
//
LOCUS       DAORQA010000352         1754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_14244_length_1754_cov_6.316657, whole genome shotgun sequence.
ACCESSION   DAORQA010000352 DAORQA010000000
VERSION     DAORQA010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14244_length_1754_cov_6.316657"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PK736_09825"
     CDS             <1..333
                     /locus_tag="PK736_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRC33726.1"
                     /translation="ALGATETVTWKYFADTADAINELKSQQYFIISIEQAKGSIMLNN
                     FVPEQGQKIALVFGHEVQGVQQSIIDVSDACIEIPQIGTKHSLNVSVCSGIVVWDIWS
                     KMNKHQSA"
     gene            complement(382..>1754)
                     /locus_tag="PK736_09830"
     CDS             complement(382..>1754)
                     /locus_tag="PK736_09830"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRC33727.1"
                     /translation="HRSVDFGLDREKYLGDGVITAYGNVNGRLVYAYSQGFTVFGGSL
                     SEAHATKICRIMDLAMQNGAPIVGLNDSGGARIQEGVVSLGGYADIFYRNTIASGVVP
                     QLSAIMGPCAGGAVYSPAITDFILMVEQTSYMFVTGPNVVKTVTHEEVTSEELGGAIT
                     HATKSGVTHFACANEIECIQTLKKLLSYMPQNCEDDAPALNYTLQNEDRPKLESIIPA
                     NPNQPYDMRDVIKQIVDDEFLEVHALYAENMVVGFARLAGKSIGIVANQPAVLAGVLD
                     INASVKAARFVRFCDCFNIPLLVLEDVPGFLPGTDQEWKGIITNGAKLLFAFSEATVP
                     RITVITRKAYGGAYDVMNSKHIGADMNYAWPSAEIAVMGAKGAAEIIFKKEISEAKVP
                     AEKLKEKELEYIEHFANPYRAAERGYIDEVILPSQTRAKLITAFGMLQNKVAKLPRKK
                     HANIPL"
BASE COUNT          497 a          404 c          357 g          496 t
ORIGIN      
        1 gcactgggtg ctaccgaaac agtgacctgg aaatattttg ctgataccgc agatgcaata
       61 aatgaattga agtcacaaca atatttcatc ataagcattg aacaggcaaa aggcagcatt
      121 atgctcaata actttgtgcc ggagcaagga caaaaaattg ctttggtgtt tggtcacgaa
      181 gtacaaggcg tgcagcaaag tataattgat gtaagcgatg catgtattga aattccgcag
      241 attggcacca agcactcact caatgtttcg gtgtgcagtg gaattgttgt ttgggatatt
      301 tggtccaaaa tgaataagca ccaatctgcc tagcattttc atttcacaga aattcaaatt
      361 tcacccttct ataaaaaaaa attacaaggg aatattagcg tgcttctttc ggggaagctt
      421 ggcaactttg ttttgcaaca ttccaaatgc tgtaataagt ttagcacgcg tttgcgaagg
      481 taaaattact tcatcgatat aaccccgctc ggcagcccga taggggttgg caaaatgttc
      541 tatatattcc aattcttttt ccttcaattt ttcggcaggt acttttgctt cgcttatttc
      601 ttttttgaaa ataatttccg cagcgccttt tgcgcccatc actgcaattt cggcacttgg
      661 ccatgcgtaa ttcatatcgg cacctatgtg tttgctattc atcacatcgt aggcgccacc
      721 ataagctttg cgtgtaatta cagtaatgcg tggcacagta gcttcgctaa atgcaaacaa
      781 taattttgca ccattggtaa taattccttt ccactcctga tcggtacccg gcaaaaaacc
      841 cggcacatct tcgagcacca gtaatggaat attaaaacaa tcgcaaaaac gcacaaagcg
      901 tgcagccttt accgaagcat taatgtcgag cactccggca agcactgcag gctgattagc
      961 aactattcca atgcttttgc ctgcaaggcg ggcaaaaccc accaccatat tttcggcata
     1021 taaggcatgc acttcaagaa actcatcgtc cactatttgc tttatcacat cgcgcatatc
     1081 gtatggctga ttgggatttg caggaattat actttctagt ttggggcggt cttcattttg
     1141 caaagtatag tttaaggcgg gcgcatcgtc ctcacaattt tgtggcatgt aactcaacaa
     1201 tttttttagc gtttgtatgc attcaatttc gttggcacat gcaaaatgtg ttacacccga
     1261 ttttgttgca tgcgtaatgg cgcctcccaa ttcttcggaa gtaacttctt cgtgggttac
     1321 cgttttcaca acattagggc cggtaacaaa catataagat gtttgctcaa ccattaaaat
     1381 aaaatcggta atggcaggag aatacacagc accaccggca cacggcccca ttatggcgct
     1441 aagttgtggc accacgcccg aagcaatcgt attgcgataa aaaatatcgg cataacctcc
     1501 caacgatact accccttcct gtatacgtgc accgcccgaa tcgttgaggc ctacaatggg
     1561 cgcaccattt tgcatagcaa gatccataat gcggcatatt ttagtggcat gtgcttcgga
     1621 aagtgaaccg ccaaaaacag taaagccctg cgagtaggca tacaccaatc tgccgtttac
     1681 atttccatag gctgtgatta caccatcgcc taaatatttt tcgcggtcga ggccaaagtc
     1741 cacactgcga tgtg
//
LOCUS       DAORQA010000353         1745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_14324_length_1745_cov_4.412426, whole genome shotgun sequence.
ACCESSION   DAORQA010000353 DAORQA010000000
VERSION     DAORQA010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14324_length_1745_cov_4.412426"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="PK736_09835"
     CDS             <1..1144
                     /locus_tag="PK736_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33728.1"
                     /translation="GYRDVYVAKLDTGGNMIWAKTYGTDETVDEAFDIVESTNGGYAV
                     TGRYIEQGTFFCMLLKTDTAGTMEYIRAYGDTNQHTVGYGIVALANGGFAITGSTTLT
                     KTSYQDYGDEFIFRINALGDTLWCHSYHGTNIDGSETGSSIVDIQGQGFAIAVPTFSY
                     PSTGFVPNKHVILRTDITGNMTMVRGYNTGSSHYNYLTKTGDLLGYIMSGFSNNYTTP
                     FANNLLRMNNNFETGCNETNLLSLTVQENPPVKIRIPPVVIASGNNVMNNTIQGLLPY
                     SIQVLCENIIDSCTTAASITQFIADENNWMQVFYNSEAQQLIVNHNSAQSNFSFNIID
                     MSGRNIESGVLQNNTINLPSNIHGIHIVQIYNKTDMHTFKIFLPNK"
     gene            complement(1151..>1478)
                     /locus_tag="PK736_09840"
     CDS             complement(1151..>1478)
                     /locus_tag="PK736_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HRC33729.1"
                     /translation="LVKRGVPDSVIISDYAGLRTLDSVLRCRDIFGQTNFTIISQQFQ
                     NERALFIAQHHNINAIAFNAKDVTAEQGLKTSLREYFARVKMFVDLYITNQQPHHSGC
                     QIAIGS"
     assembly_gap    1479..1488
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1489..>1745)
                     /locus_tag="PK736_09845"
     CDS             complement(<1489..>1745)
                     /locus_tag="PK736_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33730.1"
                     /translation="SLIICNYITIRTTQSFIYNQSQIIPYNKVALLPGTSHSLGRGIA
                     NPFFENRMDAAWQLYASGKISYIVVSGDNRTLSYNEPAQMR"
BASE COUNT          553 a          307 c          351 g          524 t
ORIGIN      
        1 cggttatcgc gatgtatatg ttgcaaaatt agataccggt ggcaatatga tttgggcaaa
       61 aacttatggt accgatgaaa ctgtggacga agcatttgac attgtggaat cgactaatgg
      121 tggttatgca gtaacaggaa ggtacattga acaaggcaca tttttttgca tgttgcttaa
      181 aacagataca gccggaacta tggaatacat tcgtgcttat ggcgatacca atcagcacac
      241 tgttggatat ggaattgttg cacttgcgaa tggcggattt gcaatcactg gttctactac
      301 attaacaaaa acaagttatc aggattatgg cgatgaattt atttttagaa taaatgccct
      361 gggcgatact ttatggtgcc acagttatca cggaaccaat atagatggta gcgaaacagg
      421 ttcgtccatt gtagatattc aggggcaggg atttgctatt gctgttccta cattttctta
      481 tccatcaaca ggttttgtac ccaacaagca cgttatttta cgaacggata taactggaaa
      541 tatgactatg gtgcggggtt acaacacagg aagttcgcat tataattatt tgacaaaaac
      601 gggagatttg ctgggttaca tcatgtcggg tttttcaaat aattacacca caccgtttgc
      661 taataatttg ttgcgtatga ataataattt cgaaaccggt tgcaacgaaa ccaatttatt
      721 gagtttaaca gtacaagaaa atcctccggt aaaaatacgt atacctccgg ttgtaattgc
      781 ttcgggtaat aatgtgatga acaatactat tcagggtttg ctgccttata gcatacaggt
      841 tttgtgtgaa aacataattg acagttgcac aaccgcagca tctataacac aatttattgc
      901 agacgaaaat aattggatgc aggtttttta caatagcgag gcacaacaat taatagtgaa
      961 tcataactca gctcaaagca attttagttt caatattatt gacatgagtg ggcgcaatat
     1021 tgaaagcggg gtgttgcaaa acaataccat caacttgcct tctaatatac atggaataca
     1081 tattgtgcag atttataaca aaactgatat gcacactttt aaaatatttt tgcctaacaa
     1141 ataaaatcaa tcaactgcct atagcaatct ggcaaccact gtgatgcggc tgttgattgg
     1201 tgatatataa atcgacaaac atttttacac gggcaaaata ttcgcgcagc gatgtcttta
     1261 atccttgttc ggccgttaca tcttttgcat tgaatgcaat ggcatttatg ttatgatgtt
     1321 gggcaataaa caatgcccgt tcgttttgaa attgctgaga aataattgta aagttggttt
     1381 ggccaaaaat atcgcgacag cgcaacacgg aatctaaagt acgtaaccct gcatagtctg
     1441 aaataattac cgaatcgggc acaccgcgtt ttacaagtnn nnnnnnnnca cgcatttgtg
     1501 ccggctcgtt ataactcaat gtacgattat cgccactaac aactatgtac gaaatttttc
     1561 cacttgcata taattgccac gctgcgtcca ttctattttc gaaaaaagga tttgcaatac
     1621 ctctgcccaa gctgtgtgag gtgcccggta acaatgctac tttattgtaa ggaataattt
     1681 gcgactgatt ataaataaaa gactgtgtgg ttcgaatagt aatgtaattg caaatgatta
     1741 aagag
//
LOCUS       DAORQA010000354         1722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_14521_length_1722_cov_5.889022, whole genome shotgun sequence.
ACCESSION   DAORQA010000354 DAORQA010000000
VERSION     DAORQA010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14521_length_1722_cov_5.889022"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PK736_09850"
     CDS             <1..469
                     /locus_tag="PK736_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33731.1"
                     /translation="QITEISLKSQRAVNRIKLFTQSSIGDNLQYLKAKMHNADDDRKH
                     MEELLNKDKMAEEYGNESAAYFNKMEDACYTAMKNYKIEPYPGKVTLFRAIDGFYNVE
                     YDEDLGWGFFAQGGCDVVNVPGTHNGIFEHPHVVELAKAMQQVLDNTYTKLPK"
     gene            481..1257
                     /locus_tag="PK736_09855"
     CDS             481..1257
                     /locus_tag="PK736_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRC33732.1"
                     /translation="MITHSTMLHCYELDDIHLQNNLDFTLNKNNTAAWLFCIDDFDLM
                     PNELLQLLSTEEKNKMLRYRQQEDRCRFLFAHAAKRILCNAYTGIGNTELEFAESQNK
                     KPYLVDSELKFNLSHSGKWIALIFQNGNDCGIDTEKIRPDFDFDFFASKNYHLRERQS
                     LSNNDGSQSRKLFFKYWARKEAWLKITGMGVFGGLPFIDTSDGNNFITIPVNEVNVSI
                     ASQYYLHSFEYENNYFCLCTTFAESNINIMNLNLLLKEYY"
     gene            1267..>1722
                     /locus_tag="PK736_09860"
     CDS             1267..>1722
                     /locus_tag="PK736_09860"
                     /inference="COORDINATES: protein motif:HMM:NF040370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HRC33733.1"
                     /translation="MNTRINLWSGPRNISTAMMYSFAQRADTTAIDEPLYGYYLKQTE
                     FIHPGYFQILESMECDLDKLKIQFTLHEYPTPLVFFKQMCHHILYNDYSWIKGMKNIM
                     LIRHPALVINSYIKVIEEISIDRIGIAAQMDLYNHLSNTNEHVVVISSQS"
BASE COUNT          593 a          281 c          320 g          528 t
ORIGIN      
        1 acagattacc gaaatttcgc taaaatcgca acgggctgta aaccggataa aattgtttac
       61 acaatcgagt attggtgaca acttacaata cttgaaagca aaaatgcaca atgccgatga
      121 cgaccgcaag cacatggaag aattgcttaa caaagataaa atggccgaag aatatggtaa
      181 tgagagtgca gcatatttta ataaaatgga agatgcatgt tatacggcca tgaaaaatta
      241 taagattgaa ccttacccgg gtaaagtaac tttgtttcgc gctatcgatg gcttttataa
      301 tgttgaatac gatgaagatt taggatgggg attttttgca caaggcggtt gcgatgtagt
      361 aaatgtgccc ggcactcata atggaatttt cgagcatcca catgtagtgg aactggcaaa
      421 agcaatgcag caggttttgg ataacacgta cacaaaactg ccaaaataat ttttctatta
      481 atgattacac attccacaat gttgcattgt tatgagttgg atgatattca tttacaaaac
      541 aatttagatt ttactttaaa caaaaataac actgcagcgt ggctgttttg cattgatgat
      601 tttgatttga tgcctaatga actacttcag ttgttatcga ccgaagagaa aaataaaatg
      661 ctgagataca ggcaacaaga agatcgatgt cgatttttat ttgctcatgc tgctaaacga
      721 attctttgca atgcgtatac aggcataggt aatactgaat tagaatttgc cgaaagccaa
      781 aataagaaac catatttagt tgacagcgaa ctgaaattta atctttcaca ttcaggaaag
      841 tggattgcat tgatttttca aaatggcaac gattgcggaa ttgacacgga aaaaattcgc
      901 cccgattttg attttgattt ttttgcatcg aaaaactatc accttcgcga gcgtcagagt
      961 ctatcaaaca atgatggctc acaatcgcgc aaattgtttt ttaaatattg ggcacgtaaa
     1021 gaagcatggc ttaaaataac aggtatgggt gtgtttggtg gccttccatt tattgacact
     1081 tccgatggca ataactttat aactatacct gtaaacgaag ttaatgtaag tattgcatct
     1141 caatattatt tacattcttt tgaatatgaa aacaactatt tttgcctgtg cacaacattt
     1201 gccgaaagta atatcaacat catgaatctt aatttacttt taaaagaata ctactaattc
     1261 aaagctatga atacaagaat aaacttatgg tcgggcccgc gaaatattag cacagctatg
     1321 atgtattctt ttgcgcaacg tgcagatacc actgctattg atgagccatt gtatggttac
     1381 tatttaaaac aaaccgaatt tattcatccc ggatattttc aaatactcga atcgatggag
     1441 tgtgatttag ataaattgaa aatacagttt acgctacatg agtaccctac tccactggtc
     1501 ttttttaagc aaatgtgtca tcatattttg tacaatgatt atagctggat aaaaggcatg
     1561 aaaaatataa tgttgattag gcatcctgct cttgtaataa attcatacat aaaagtgata
     1621 gaagaaattt ccattgatcg tataggcatt gccgcacaaa tggatttata taatcacctt
     1681 tcaaatacga acgaacatgt tgtggtaata agtagtcaaa gt
//
LOCUS       DAORQA010000355         1722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_14523_length_1722_cov_4.652070, whole genome shotgun sequence.
ACCESSION   DAORQA010000355 DAORQA010000000
VERSION     DAORQA010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14523_length_1722_cov_4.652070"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /gene="dnaB"
                     /locus_tag="PK736_09865"
     CDS             <1..1470
                     /gene="dnaB"
                     /locus_tag="PK736_09865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRC33734.1"
                     /translation="HFLFNFDSDKLLSYKRIGKTFSICWKGLFHFPDATLYNCWNTNW
                     LVNYSKKMAGGASLIAWLVPFFILKDNRKTVLLFYASALQLIGGPYYISTLTNRVASA
                     ANIEFHARIIIQKHIQRELIRISTETIKDAFEDSTDVLELLDKAERNLFAIAEGNLRK
                     QYDNMQTLIGKAIKEYERAAEQPDGVIGVPSGFSDLDRITSGWQKSDLIILAARPGMG
                     KTAFVLSLARNASVQFRKPVALFSLEMSSVQLVNRLISSETEIGGEKLRKGDLTDAEW
                     QQLNKKIQPLENAPLYIDDTPAITIFELRAKCRRLVAERKVELIIVDYLQLMQAGSEN
                     GRGNREQEISTISRSLKSIAKELEVPIICLSQLSRAVESRGGVKKPMLSDLRESGAIE
                     QDADMVMFIYRPEYYENTQDAQGMSTAGLAEIIVAKNRNGSLSTVNLRFIGHLTKFAD
                     WDNYAMVDDGAQFATMGADQPRVSKFSRMDDLPNEDAPF"
BASE COUNT          523 a          354 c          364 g          481 t
ORIGIN      
        1 catttcttat tcaatttcga ttcggataag ttgctttcat ataaaagaat aggaaaaaca
       61 ttttctattt gttggaaggg gctgttccat tttcctgatg ctactcttta taattgttgg
      121 aataccaatt ggcttgtaaa ctattctaaa aaaatggcgg gtggtgcatc gcttattgca
      181 tggcttgttc cgttttttat tttgaaagat aatcgcaaaa ctgttttgct tttttatgct
      241 tcggcattgc agcttatagg aggaccatat tatatatcca cactcaccaa cagagttgca
      301 tcggccgcca atatcgaatt tcatgcgcgt ataattattc aaaaacatat tcagcgcgag
      361 ttaatacgca tttctaccga aactataaaa gacgccttcg aagactcgac cgatgtactt
      421 gaattactag ataaagccga aagaaatctt tttgcaattg ccgaaggaaa tttgcgcaag
      481 caatacgaca acatgcaaac ccttattggc aaagccataa aagaatacga acgcgcagcc
      541 gaacagcccg atggtgtgat tggtgtgcca agtggattca gcgatttgga tcgcattaca
      601 tcaggttggc aaaagagcga tttaataatc ttggccgcac gccccggtat gggaaaaaca
      661 gcgtttgtac tttcgcttgc ccgcaatgcc tcggtacaat ttcgtaagcc tgttgcattg
      721 ttctctctgg aaatgtcatc ggtgcaattg gtaaatcgtt taatatcgag cgaaaccgaa
      781 attggtggcg aaaaactgcg taagggcgac ctcaccgatg ccgaatggca acaactaaat
      841 aaaaaaatac aaccgctcga aaatgctccg ctttatattg acgatactcc ggccattact
      901 atttttgagt tgcgtgcaaa atgccgaaga cttgtggcag aacgtaaagt tgaattaata
      961 atagttgact atttacaatt gatgcaagcc ggcagcgaaa acggacgtgg caatcgtgag
     1021 caggaaataa gtaccatatc gcgttcgcta aagagtatag ccaaagaatt ggaagtgcct
     1081 ataatctgcc tttcgcaatt gagtcgtgcg gtggaatcgc gcggtggcgt aaaaaagcct
     1141 atgctgagcg acttacgcga atcgggtgcc atagagcaag atgccgatat ggtaatgttt
     1201 atttaccgac ccgagtatta cgaaaacact caagatgctc aaggtatgtc gactgcaggc
     1261 ctggccgaaa taatagttgc caaaaaccgc aatggctcgc taagcacagt aaacctccgc
     1321 tttataggcc accttacaaa atttgccgac tgggataact atgcaatggt tgatgatgga
     1381 gcacaatttg caactatggg tgccgaccaa cctcgtgtat caaaattctc ccgcatggac
     1441 gatttgccaa atgaggatgc acctttctaa tttaataaaa aaattggatt ttatcaaaaa
     1501 aacgcttggc ctcgcgcacc cgtaacccca ataacattaa gttaagagaa tttgattttt
     1561 atcccgtagg gattaccgct tggtaaaaat aaatgcaaaa cggaattttg ttccgtagga
     1621 actacccttt cttcaatcat cattttgcat cattgggttg tccttacagg catacacgtc
     1681 aacttaagga atacttagct cgttattttt ttttaagagg ca
//
LOCUS       DAORQA010000356         1708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_14646_length_1708_cov_21.045372, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000356 DAORQA010000000
VERSION     DAORQA010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14646_length_1708_cov_21.045372"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            451..>1708
                     /gene="atpD"
                     /locus_tag="PK736_09870"
     CDS             451..>1708
                     /gene="atpD"
                     /locus_tag="PK736_09870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRC33735.1"
                     /translation="MSKGKVAQVIGPVVDVSFDTTTSKLPNILEALEVTKGNGQVVVL
                     ECQRHVGEDTIRAIAMDSTDGLVRGMEVTATGTTIAMPGGEGIKGRLFNVVGEAVDGM
                     RTVTKDIKLSIHRDPPKFEDLSTETQVLFTGIKVIDLLEPYAKGGKIGLFGGAGVGKT
                     VLIMELVNNIAKAYSGLSVFAGVGERTREGNDLLREFLESGVINYGDEFKHSMESGGW
                     DLSKVDYEKLKESKATLVFGQMNEPPGARARVALSGLTVAEHFRDGDGTSTGGRDILF
                     FIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLASEMGAMQERITSTKRGSITSVQAVY
                     VPADDLTDPAPATTFAHLDATTVLSRKIAELGIYPAVDPLDSTSRILSAAVLGNEHYD
                     CAQRVKLTLQRYKELQDIIAILGMDEL"
BASE COUNT          499 a          328 c          380 g          501 t
ORIGIN      
        1 acgttgattt tgctatcgca aaatttatgc atgcccaatt gttatttatt aattgtaatg
       61 ctattaagtc ctttgcaaaa ttgaaatgca ccagattcat tgcacaaata ttttttgaga
      121 aagacctctt tgataaattg attccattaa agcataagta aataacaatc taaaatttag
      181 aaatacttct acggtttaat caaagggaga aatcaacttg attacacttt tcaagccatt
      241 tttactatca tattatttat gtcgtcttgc tcaaaaatgg gcttttcaac taatgtttat
      301 aaaaaacgaa agcatttcga aattttatta atagcagttg tacaactaaa gcaacaatta
      361 ttaccttcgc gcccgatttg aggggcattg cctgataaat acagttgaag ccttcggata
      421 cttaaataaa aacaattatt tttcacaaat atgagcaaag gaaaagtagc acaggtaatt
      481 ggtcctgtag tggatgtatc attcgataca acaacatcaa aacttccaaa cattttagaa
      541 gcccttgagg tgacaaaagg aaatggtcag gtagtagtac ttgagtgtca gcgtcacgtt
      601 ggcgaggaca caatacgtgc aattgccatg gactctaccg atggtttggt gcgtggtatg
      661 gaagttactg caacaggaac aaccatagca atgccgggtg gcgaaggtat caaaggtcga
      721 ttgtttaatg ttgtgggcga agctgtggat ggcatgcgca ctgttaccaa agacataaaa
      781 ctttctattc atcgcgaccc acctaagttt gaggaccttt ctacggaaac acaggtatta
      841 tttacaggta ttaaagttat cgatttgctc gagccatatg caaagggtgg taaaattggt
      901 ttgtttggtg gtgccggtgt aggtaaaacc gttttgatta tggagctggt aaataatatt
      961 gccaaagctt attcaggttt gtcggtgttt gccggtgtgg gtgagcgtac ccgcgaagga
     1021 aacgacttat tgcgcgagtt ccttgagtca ggcgtaatta actacggtga cgaatttaaa
     1081 cacagcatgg aaagtggcgg ttgggatttg agcaaagtag attacgaaaa acttaaagaa
     1141 tcaaaagcta cacttgtatt cggacaaatg aacgagcctc cgggagcacg tgcacgtgtg
     1201 gcgttgagcg gacttacagt tgccgaacat ttccgcgatg gcgatggtac atcaaccggt
     1261 ggccgcgata tccttttctt tatcgacaat atattccgct tcactcaggc aggttccgag
     1321 gtatcggccc ttcttggtcg tatgccatca gccgtgggtt atcaacctac attggcttcg
     1381 gaaatgggtg ccatgcaaga gcgtatcaca tctaccaaac gtggttctat cacatcggta
     1441 caagcggtgt acgtacctgc cgatgactta actgaccctg ctccggctac tacatttgcc
     1501 cacttagatg ccactacggt attgagccgt aagattgccg agttgggtat ttatcccgct
     1561 gtggatcctt tggattctac atcacgtatc ctttctgctg cagtattggg caacgaacat
     1621 tatgattgtg ctcaacgcgt gaaattaact ttgcaacgtt acaaagaact tcaagatatt
     1681 attgccatcc ttggtatgga tgaattga
//
LOCUS       DAORQA010000357         1708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_14648_length_1708_cov_7.183303, whole genome shotgun sequence.
ACCESSION   DAORQA010000357 DAORQA010000000
VERSION     DAORQA010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14648_length_1708_cov_7.183303"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..>1708
                     /locus_tag="PK736_09875"
     CDS             72..>1708
                     /locus_tag="PK736_09875"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRC33736.1"
                     /translation="MALPQKLKLSGQINSAETHLPVPDALIMLQPGEHKAVTDEKGNF
                     QFNNLSAYNYQLHVYRFGYSKMEQNISLHADTTINFDMKFSGIDLAEIKVNGANENNS
                     KETITDIDKLLRPVNTTQDLLTLVPGLFIAQHAGGGKAEQIFLRGFDSDHGTDFNISI
                     DGLPVNMVSHAHGQGYADFHFVIPETVEKLNVYKGPYNAKFGDFSTSGTGEFFTKNAI
                     ANNEIKLEAGMFDTYRCMTMIKLLDKKHLLTKKKENAFVAGEYVFTNSYFLAKQKFNR
                     YNLFGKYNAMLNDKNQFTFSASTFSAHWNASGQVPQRAVESGDISIYGSIDPSEGGQT
                     NRSNVNATISTTLSNNMLIKNQLYYVNYYFNLYSNFSFFLNDSINGDEINQTDKRNIF
                     GYSGTLQNEYLIGDKKLMFNAGLGSRNDIANIMLRNAVKREITDTVVTGKVIQSNINS
                     FVDFTLDISKQFSINTGLRFDYFRFQFNDNKVQENSGTKSIIRGSPKLNFFYSVKDGL
                     QLFVKSGIGFHSNDARSVVINKINNSLPRAYGAEFGS"
BASE COUNT          600 a          287 c          316 g          505 t
ORIGIN      
        1 atttgaaagt ggatacaata cttaagcata aaaagtcgat agcattattt gtgttaatgt
       61 gcatttgcac aatggcattg ccacaaaaat taaaactgag tgggcaaata aattctgccg
      121 aaacacattt gcccgttccc gatgcattga ttatgttaca acctggcgag cacaaagctg
      181 taacagatga aaagggaaat tttcaattta ataatttatc agcttataat tatcagcttc
      241 atgtatatcg ttttgggtat agtaaaatgg aacaaaatat ttcgctccat gctgacacaa
      301 ctattaattt cgatatgaaa ttttcgggaa tagatttagc agaaataaaa gtaaatggtg
      361 caaatgaaaa caacagcaaa gaaacaataa ctgatataga caagttattg cgaccggtaa
      421 atactacaca ggatttacta acgctggtgc caggtttatt tattgcacaa catgcaggtg
      481 gtggcaaagc agaacaaata tttcttcgtg gatttgattc tgaccatggt acagatttta
      541 atatttcgat tgatggctta cctgtgaata tggtatcgca tgctcatggg cagggctatg
      601 ccgattttca ttttgtgatt cccgaaacgg tagaaaaact aaatgtttac aaaggcccat
      661 acaatgccaa gtttggcgat ttttccacat cgggcaccgg agagtttttt acaaaaaatg
      721 ctatagccaa taatgaaata aaattagaag caggtatgtt tgatacttac aggtgcatga
      781 ccatgataaa attgctcgac aagaaacatc ttcttacaaa gaaaaaagaa aatgcttttg
      841 tggccggaga gtatgtattt acaaattcat attttttagc aaaacaaaaa ttcaatcgct
      901 ataatttatt tggaaaatat aatgccatgt tgaatgataa aaaccaattc acattctcgg
      961 catctacttt ttcggcccac tggaatgcat cggggcaggt accacaacgt gcagtagaat
     1021 cgggtgatat aagcatttac ggcagcattg atcccagcga aggtggacaa acaaatcgca
     1081 gtaatgttaa tgcaactatt tccaccacac tcagcaataa tatgctgata aaaaatcaac
     1141 tttattatgt gaattattat ttcaatctct attcaaactt ttctttcttt ctaaatgata
     1201 gcataaatgg tgatgaaata aatcaaactg ataagcgtaa tatttttggt tatagcggca
     1261 cattgcaaaa cgaatattta attggcgata aaaaattaat gttcaatgcc ggattgggat
     1321 cgcgaaatga tattgcaaat attatgttgc gcaatgccgt gaaaagagaa attaccgaca
     1381 ctgttgttac aggcaaagtc attcaaagca acataaattc ctttgtagat tttacgctcg
     1441 atataagcaa gcaattttcc attaatacag gtttgcgatt cgattatttt cgcttccagt
     1501 ttaacgacaa taaagttcag gaaaattcgg gaactaaatc aataatacga ggtagtccaa
     1561 aattaaattt cttctatagt gtgaaagatg ggttacaact tttcgtaaaa tcaggaatag
     1621 gatttcactc caacgatgcc cgttcggtag taataaacaa aataaacaac agccttccgc
     1681 gcgcctatgg tgccgaattt ggcagcga
//
LOCUS       DAORQA010000358         1707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_14662_length_1707_cov_5.338378, whole genome shotgun sequence.
ACCESSION   DAORQA010000358 DAORQA010000000
VERSION     DAORQA010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14662_length_1707_cov_5.338378"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1432
                     /locus_tag="PK736_09880"
     CDS             38..1432
                     /locus_tag="PK736_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HRC33737.1"
                     /translation="MKNRYIDLIKQTFDFPQEGFDVIDDCLHFNGIPVMDIIEQYGTP
                     LKISYLPKIGAQINKAKRIFNVAMARNDYKGSYTYCYCTKSSHFSFVMEEALKNQIHL
                     ETSSGFDIQIIRNLHEQGLVNKEMFILCNGFKRDNYIQGINDLIKDDFVNVIPILDNM
                     AELDGYEKNIKKKFKIGLRIAADEEPNFEFYTSRLGIRYKDIIPFVKDRIKNNQYTEL
                     KMLHFFINTGIKDNAYYWSELSKGIDMYCELKQICPELDSLDIGGGWPIRTSLSFDYD
                     YDYMANEIIKQIKSTCEKNNVAEPNIFTEFGSFTVGESGAVLFKTINQKTQNDKETWN
                     MIDSSFITTLPDTWGINQKFILLAVNHWDKDYQRVNLGGLTCDSEDFYSFESQTNRVF
                     MPRAGDSEEPLYLGFFHTGAYQEALGGYGGIQHCLIPAPKHVLIDRDANGEYTTRLFA
                     KEQSAKSMLKILGY"
BASE COUNT          523 a          280 c          362 g          542 t
ORIGIN      
        1 atctaagtta tttacattta atattatttt gtctcttatg aagaatcgct acatagattt
       61 aattaaacaa accttcgatt ttcctcagga aggcttcgat gtgattgatg attgcttaca
      121 ttttaatggt ataccggtga tggatatcat tgagcaatat ggcactccgt taaaaatttc
      181 ttaccttcct aaaataggtg ctcaaataaa caaagccaag cgaatattta atgtggctat
      241 ggcacgaaac gattacaaag gtagttatac ttattgttat tgtaccaaaa gttctcattt
      301 ttcattcgtt atggaagagg cattgaaaaa ccaaattcac ctcgagactt cttcaggatt
      361 tgatattcag attattcgca atctacatga gcaaggtttg gtaaacaagg aaatgtttat
      421 tctgtgcaat ggatttaagc gcgataatta tattcagggc ataaatgatt tgataaaaga
      481 tgactttgta aatgtgattc cgatactcga taatatggcc gagcttgatg gctatgaaaa
      541 aaatataaaa aagaaattta aaataggttt gcgcatagct gccgatgaag aaccaaactt
      601 cgagttttat acttcgcgcc tgggcattcg atataaagat attattccgt ttgtaaaaga
      661 ccgcatcaag aataatcaat atacagaatt gaaaatgttg cactttttta tcaataccgg
      721 tataaaagac aatgcatatt actggagcga attgtcgaaa gggatagata tgtattgcga
      781 gttgaaacaa atttgccccg agctcgattc gctcgatatt ggcggaggat ggcccattcg
      841 cacttcactc agtttcgatt atgattacga ttatatggcc aacgaaatca taaagcaaat
      901 aaaatcgacc tgcgaaaaaa ataatgtggc cgagccaaat atttttacag aatttggtag
      961 ttttacagtc ggggagagtg gcgctgtttt gtttaaaacc atcaaccaaa aaacgcaaaa
     1021 cgataaggaa acatggaaca tgatagatag ttctttcatt accacattgc ccgatacatg
     1081 gggcataaat cagaaattta ttttattggc ggtgaatcat tgggataaag attatcaacg
     1141 tgtaaacctt ggcgggttaa cttgcgatag cgaagatttt tatagtttcg agtcgcaaac
     1201 aaaccgtgtg tttatgcccc gtgcgggcga tagtgaagag cctttgtact tagggttttt
     1261 tcataccggt gcctatcagg aagcgctggg tggttatggt ggtatacaac attgccttat
     1321 acctgcacca aagcatgtgc tgatagatcg cgatgccaat ggtgaatata ctacgcgcct
     1381 ttttgcaaaa gaacaaagtg ctaaatcgat gctgaaaatt ttggggtact aggggggagg
     1441 ggattagttt atagttttta gttcttagtt cttagttttt agtttatagt ttttagttct
     1501 tagtttcgtt tgcggagagg attgttctta gtcccgacac tacggtcggg atgccgacca
     1561 aagtgtcggc attcttagtt acgtttgcgc agaggaattt tcttcgatcg gcattgcgaa
     1621 ttgtcgcagt gagtattctc gaactgcgag gcaatctctg cgtagaaatt agtttttgtt
     1681 tttaatctcg ttctctcaca aattagt
//
LOCUS       DAORQA010000359         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_14871_length_1687_cov_5.204657, whole genome shotgun sequence.
ACCESSION   DAORQA010000359 DAORQA010000000
VERSION     DAORQA010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14871_length_1687_cov_5.204657"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="PK736_09885"
     CDS             complement(<1..1368)
                     /locus_tag="PK736_09885"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC33738.1"
                     /translation="MKKILLFMCSLMCCAMLHAQTGTITGVVANEATGAGLSGLNVTV
                     IENGATTVTNVDGGYSFSNLPYNTYTIQISADGFEATTLSVKVDKATVDAGRTTLHVD
                     DNKGAIDNIPTVTISESDLKETSSAAISSALSASRDVFIAAISFNFSALRFRFRGYDD
                     ENFITLMNGAPMTDLTNGRTAYFTWSGLNDVMRSREANLGLNAASYTYGNIGGSYFID
                     SRASKQRKQIQASYSLSNRSYDNRFMLTYGSGWMKGGWAVSGSVSRRWANEGFIRGTF
                     SDGYSYFLAVDKKINNKHMLSLTTFAAPNKTGRSAPATQEQIDLAGDVFYNSAWGYQN
                     GKVRNINVAKNFEPTTILTHEWKISDRTQLVTAASMLMGTTSRTAFDWYNAPDPRPEY
                     YKNLPSYYETPAIAQELAAYLKANPDAMQIQWDELYEANRLNNVEFKNVDGIAGNNIT
                     GKRSLY"
BASE COUNT          500 a          333 c          333 g          521 t
ORIGIN      
        1 atatagtgaa cgcttacccg taatattatt tccggctatg ccatctacat ttttaaattc
       61 aacattatta agtctgttag cttcgtacaa ttcatcccat tgtatttgca tggcatcggg
      121 gttggctttt aagtaagctg caagttcctg tgctattgca ggggtttcat aatagctggg
      181 taaattttta taatactcag ggcggggatc aggagcattg taccaatcga aagccgtgcg
      241 cgaagtagtt cccatcagca tagatgcggc agttaccagc tgtgttctgt cactaatttt
      301 ccattcatgt gtaaggatcg tggtaggttc aaaattcttg gccacattta tattgcgaac
      361 tttaccattt tgatatcccc acgctgaatt gtaaaacaca tcgcctgcca aatcaatttg
      421 ctcctgagta gcaggtgccg aacgtcctgt tttgtttggt gctgcaaaag tggttaagct
      481 cagcatgtgc ttgttattta tttttttgtc cacagcaaga aaataactgt acccgtcaga
      541 gaacgttccg cgaataaatc cttcgttggc ccatctgcgc gacacgctgc ccgatactgc
      601 ccatccacct ttcatccagc ctgagccata tgttagcata aagcggttat cgtaagagcg
      661 attgcttaac gagtatgaag cctgaatttg cttgcgttgt ttagatgcac ggctatcaat
      721 aaaatacgaa cctccaatgt taccatatgt atatgatgca gcattcaaac ccaggttggc
      781 ctcacgactg cgcattacat cattcaatcc actccatgtg aagtatgctg tacggccgtt
      841 ggtaaggtct gtcatgggcg caccattcat aagggtgata aaattctcgt catcgtatcc
      901 gcgaaagcga aatcttagtg cactaaagtt aaaagaaatg gcggcaataa aaacatcgcg
      961 cgatgcactc aatgcactgg aaatggctgc cgatgaagtt tcttttaaat cactctccga
     1021 tatggttacc gtaggaatat tatcgatggc gcctttgtta tcatctacat gcaacgtggt
     1081 gcggccggca tcaacagtgg ctttatctac cttaactgaa agggttgtag cttcaaatcc
     1141 atcggcactt atttgtattg tataagtgtt gtagggcaag ttgctaaagg aatagccacc
     1201 atcaacattg gttacagtag tggcgccatt ttctataacg gttacattta gtccactcaa
     1261 accagcgcct gtagcctcat tagccactac tccggtgata gtgccggttt gtgcatgcaa
     1321 catagcacaa cacataaggc tgcacataaa aagtaaaatt ttcttcatct tttgttttta
     1381 tcttagggat ggcaaatgta aaaaaataat gtagccataa aagactttgt ttattaactt
     1441 aatgtgtaca aaagtttgtg aatactatgg tattattttg ctaatgaaaa gataacaaaa
     1501 cgaaagagta tggtatgttt caaaacaaaa taaacatata tagttattgt tatgtcaaaa
     1561 gaatatttga ggaaaataat ccggaactaa aatttgattt caaactatat ttacctgaac
     1621 atttttgtaa taatagttaa aaagccatta catgatttaa aatattcgta tggaaaaatt
     1681 tcattta
//
LOCUS       DAORQA010000360         1675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_14992_length_1675_cov_8.434568, whole genome shotgun sequence.
ACCESSION   DAORQA010000360 DAORQA010000000
VERSION     DAORQA010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14992_length_1675_cov_8.434568"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1675
                     /locus_tag="PK736_09890"
     CDS             <1..>1675
                     /locus_tag="PK736_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33739.1"
                     /translation="RFVFNSDGVITRDGLLIDDFSISDYSNTSMDITPQLYAPPCNSP
                     QDIGIIFTASTFDTVNTTGTVDSLKIFFGDGTDTTLSFNYDSNYVYVKIMHKYTTTGN
                     FSLQAICGVDGLPAITTTLLNAIYIDPFCGNISGKVYLDKNADCIFNNNDDSLANQLV
                     RIKYSTGQLYYASTDSSGNYAFTVPNSLSYEVTLNNAQLPLYSIVCPNSGMYALSTLP
                     ATNIDFSLTCNTGYDLASIIHTQNVRPLIPTIIYLSFIQYSCYDSIATGKMILDSNLN
                     FVSADVIPDSINGDTLLWQLPVSGQVLLKFIEVTVLPDSILQLGDTVCNTLIIEPIAG
                     DIDPANNTYISCGAVTNSFDPNAKEVIPDGDIDAGQLLLYTIYFQNTGTAPALNVRIV
                     DTLSNNLEIGSLQIINSSHSMHMNILPSRVAHFVFDNINLPDSNTNEPQSHGFVTYAV
                     KAKTSLIGGDEIKNTAHIYFDFNSPIVTNTTYNFLDTIIALSPNLISQKQMVLFPNPA
                     STSVNFYLPNNAIARKVTIQNILMQQTVIMQNNDNTIDVSGMVSGFYFVN"
BASE COUNT          529 a          321 c          285 g          540 t
ORIGIN      
        1 cgtttcgtgt ttaattccga tggagttata acacgtgatg gcttgttgat agacgatttt
       61 tccatcagtg attacagtaa tacatcgatg gacatcacac cacaattata tgcacctcca
      121 tgcaattctc cacaggacat tggtataata tttaccgctt caaccttcga tactgtaaat
      181 acaacaggaa ctgttgattc actaaaaata ttttttggcg atggtaccga tactactttg
      241 agttttaact acgattccaa ttatgtgtat gtgaaaatta tgcataagta tacaacaacg
      301 ggaaattttt ctttgcaagc tatttgtggt gttgatggtt tgcctgctat aacaactact
      361 ttactgaatg ctatttatat cgatccattt tgcggcaaca tttcagggaa agtatattta
      421 gataaaaatg ccgattgcat ttttaataac aatgatgatt cacttgccaa tcaacttgta
      481 agaataaaat acagcacggg acaattgtat tatgcaagca ccgatagctc gggtaattat
      541 gcctttactg ttccaaactc attgagttat gaagtaacat taaacaatgc acaactgcct
      601 ttgtacagta ttgtttgtcc caattcgggc atgtatgcat tatcaacgct acctgctact
      661 aatattgact tttcacttac gtgcaacact ggttacgacc ttgcatcgat cattcacaca
      721 caaaatgttc ggccattaat tcccacaatt atatatttaa gttttattca atacagttgc
      781 tacgattcta ttgccactgg aaaaatgata ctcgattcaa atttaaattt tgtatcggcc
      841 gatgtgatac cagacagcat aaatggcgat acgctattgt ggcaactgcc tgtttcgggt
      901 caggtacttt taaaatttat tgaagtcact gtattgccag attcaatttt gcaattgggc
      961 gatactgttt gtaatacgct tattattgag ccaatagccg gtgacattga tcctgctaat
     1021 aacacttata tctcttgcgg tgctgttaca aattctttcg acccaaatgc taaggaagta
     1081 atacctgatg gagatattga tgcaggacaa ctattactgt ataccatcta ttttcaaaat
     1141 acaggaactg caccggcact caatgttcgc attgtagata cactgagcaa caacttagaa
     1201 attggcagtt tgcaaataat aaattccagt cacagcatgc atatgaacat ccttccctcg
     1261 cgggtggcgc attttgtttt cgacaatatt aatttgcccg attcaaacac gaatgaacct
     1321 caaagccatg gttttgttac ctatgcagtg aaagcaaaaa ccagtttgat tggtggtgat
     1381 gaaataaaga atactgcaca tatttacttt gatttcaata gccctatagt tacaaataca
     1441 acatataatt ttctggacac tattattgcg ttgtcaccaa atttaatttc tcaaaagcaa
     1501 atggtgctgt ttccaaaccc tgctagcaca agtgtaaact tctatttgcc aaataatgca
     1561 atagcaagaa aagttaccat tcaaaacata ctcatgcaac aaacagtaat tatgcagaac
     1621 aacgacaata ctatcgatgt atccggaatg gtgagtggtt tttattttgt aaatg
//
LOCUS       DAORQA010000361         1674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_15005_length_1674_cov_6.606547, whole genome shotgun sequence.
ACCESSION   DAORQA010000361 DAORQA010000000
VERSION     DAORQA010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15005_length_1674_cov_6.606547"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1674
                     /locus_tag="PK736_09895"
     CDS             <1..>1674
                     /locus_tag="PK736_09895"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC33740.1"
                     /translation="SGIITDGNKNAIVGASIQLEPTMYATTSDASGKFYLNKIRPGTY
                     TVKVFRLGYKSYEAVIEILQAYEIQIVLQPVLYINNEITVTALRKGNSDMMTSVTINK
                     EEIEERNTGVDIPFLLEQTPSVIATSDAGTGIGYTGLRIRGTDATRINVTLNGVPLND
                     AESQQLYWVDLPDVASSMQNIQVQRGVGTSTNGGSAFGGSVNMLLQRETEKPYSTVQL
                     ASGSFNTFKRNISFGTGLIKNHFYFNGRLSKINSDGFVDRSSADLQSYMLQTGYAGQR
                     FSLHVMMMDGKEKTYQAWNGIPESRWNNDTQGMLAYIIRNGLDSTEAFNLLNSGTTYN
                     YFDYKNQTDNYRQTHYQLQTVFDINAKNLLQVSLHYTKGRGYYEEYKKQQTLADYSIT
                     NTVYDGDTILESDIIRQKWLENDFYGFIFSISSQVNTKLELINGGGYNIYDGKHFGYV
                     IWYQFVNPKLDPVRYYSDYAIKHDYHYFLKAIYTYNKWKALIDLQYRGVKYSFEGKNE
                     LLMLSQQKANLHFFNPKAGLSYAPNQRETGFVSVSVGNKEPSRDDYVNSS"
BASE COUNT          551 a          293 c          346 g          484 t
ORIGIN      
        1 tcgggaataa ttactgatgg gaacaagaat gcaattgttg gtgcatccat ccaattggaa
       61 cctactatgt atgcaaccac aagtgatgct tcgggaaaat tttatctcaa caaaattaga
      121 cccggtacct atactgtaaa agtttttagg ctgggatata aatcctatga agcagttatt
      181 gaaatattgc aagcatacga aatacaaata gtattgcaac ctgtattata cataaataat
      241 gaaattactg taacagcgct gcgcaaagga aacagtgata tgatgacttc ggtaacaatt
      301 aataaagagg aaattgaaga acgaaataca ggagttgata ttcctttttt actggagcaa
      361 actccatctg taattgctac ctccgatgca ggcaccggaa ttggttatac cggattgcgc
      421 attcgcggaa ctgatgccac acgtattaat gttacactca atggtgtacc cttgaacgat
      481 gctgagtcgc aacaattgta ttgggtcgat ttgcccgatg tggcttcttc tatgcaaaac
      541 atacaggtgc aacgcggtgt gggaacatct accaacggag gttcggcatt tggtggctcg
      601 gtaaatatgt tgttgcagcg cgaaaccgaa aagccatatt caacggttca acttgcaagt
      661 ggatcgttta atactttcaa acgcaatatt tcatttggta cgggcttgat aaaaaatcat
      721 ttttatttta atggtcggtt gtcgaaaata aatagcgatg gatttgtaga tagaagtagt
      781 gctgacttgc aatcgtacat gctgcaaaca gggtatgccg gtcaacgttt ttctttacat
      841 gtaatgatga tggatggaaa ggaaaaaaca tatcaggcct ggaatggtat tcccgagtcg
      901 cgctggaaca atgatactca aggaatgctg gcgtacatta tccgtaatgg actcgatagc
      961 accgaggctt tcaacttgct aaacagcggt actacttaca attatttcga ttataaaaac
     1021 caaaccgata attaccgtca aacacattat caattgcaaa cggtatttga tattaatgca
     1081 aaaaaccttt tacaggtatc cctgcattat accaaaggac gtggatatta tgaggagtat
     1141 aaaaaacagc aaacattagc tgactacagt ataaccaata ctgtatacga tggagatacg
     1201 attttggaaa gcgatattat caggcagaaa tggctcgaaa acgattttta tggttttatt
     1261 ttttcgatta gttcgcaggt taataccaaa ctcgaattga taaatggcgg agggtataat
     1321 atttatgatg gcaaacattt tggctatgtc atttggtatc agtttgtaaa tcccaaattg
     1381 gaccctgtgc gatattatag cgattatgca ataaagcatg attaccatta tttcttaaaa
     1441 gcaatttata cttacaataa atggaaagca cttattgatt tacaatatcg tggtgtgaaa
     1501 tattctttcg aaggcaaaaa cgagttgtta atgttatcgc agcaaaaggc aaacctccat
     1561 tttttcaacc caaaagccgg attaagttat gcgcccaacc aaagggaaac cggttttgta
     1621 agtgtgagtg ttggcaacaa agaaccatcg cgcgatgatt atgtaaactc ctct
//
LOCUS       DAORQA010000362         1672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_15029_length_1672_cov_5.294991, whole genome shotgun sequence.
ACCESSION   DAORQA010000362 DAORQA010000000
VERSION     DAORQA010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15029_length_1672_cov_5.294991"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>307
                     /locus_tag="PK736_09900"
     CDS             <1..>307
                     /locus_tag="PK736_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRC33741.1"
                     /translation="IAAGNECLIVGDVKQSIYRWRGGNMHLLATQVVKDLQHFDGLIT
                     TKQLNTNYRSAENIVSFNNFLFGASAFVLQNEVENTTLELIKSTYGEDFVTQQLSPT"
     assembly_gap    308..328
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <329..1385
                     /locus_tag="PK736_09905"
     CDS             <329..1385
                     /locus_tag="PK736_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRC33742.1"
                     /translation="DWIENKRKKADEKNNENDVEIEDENFNTLTLNNLHQDIQLVIEK
                     GFKYKDITILVRTKANGATVADYLQQHGIINLITPESFYLASSIKISFIIAYLEFCNN
                     AHNVIAKHTMLRCYNQLFQLENDNTDFEILFSQFIETGKHNAHGVDEMAIALINHFKI
                     NTTPDAYITRFMDVLHQLRNTNIITLPAILQWWADNKYSDAASVILPEDYNAISIMTI
                     HKSKGLQFPIVMMPFLNWSVKPKSNEVIWVQTPDEPFNNSIIPVNISEKKMLLSSFAY
                     DYNIEKDNTIIDSLNTLYVAFTRAEQALFIYTQQLAESPTINKINTLLSNLINISELR
                     VHHQRAVVQLQHPCIYR"
     gene            complement(1312..>1672)
                     /locus_tag="PK736_09910"
     CDS             complement(1312..>1672)
                     /locus_tag="PK736_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRC33743.1"
                     /translation="KDVEIIAGGNYDKSKGYFIEPTILLAKKPDYITMCEEIFGPVLT
                     IYVYDEKKYEETLDLVDRTGTYALTGAIIAHDRYAIELATRKLEHAAGNFYINDKCTG
                     AVVGQQPFGGGRAIQKY"
BASE COUNT          539 a          315 c          288 g          509 t
ORIGIN      
        1 ttattgctgc cggcaacgaa tgtttaatag tgggggatgt gaagcaaagt atataccgct
       61 ggcgcggagg aaatatgcat ttgctcgcaa cacaggtagt taaagatttg cagcattttg
      121 atgggttgat tactaccaaa caactaaaca ccaattatcg cagtgccgaa aatattgtaa
      181 gctttaataa ttttttattt ggtgcatccg catttgtgtt gcaaaacgag gttgaaaaca
      241 ctacccttga attgataaaa tctacttatg gcgaagattt tgtcacccaa caattatctc
      301 cgacaaannn nnnnnnnnnn nnnnnnnntg actggattga aaacaaacga aaaaaggccg
      361 atgaaaaaaa taatgaaaac gatgttgaaa ttgaagatga aaatttcaac acgcttacac
      421 taaataattt acatcaagat attcaacttg taattgaaaa aggattcaag tataaagaca
      481 ttaccattct tgttagaaca aaagcaaatg gcgccaccgt tgccgactat ttgcaacagc
      541 atggtattat aaatttaatt actcccgaat ctttttacct ggcatcatca ataaaaatta
      601 gtttcataat tgcttatctc gaattttgta acaatgcgca taatgtaatt gcgaagcata
      661 caatgttgcg ttgctataat caactcttcc aattagaaaa tgacaacaca gattttgaaa
      721 tcctgttttc gcaatttata gaaaccggta aacacaatgc acacggagta gatgaaatgg
      781 ctattgcact tatcaaccat tttaaaataa acactacacc cgatgcatac attactcgtt
      841 ttatggatgt attgcaccag ttgcgcaata caaatattat taccctgccg gcaatattgc
      901 aatggtgggc cgataataaa tacagtgatg cagcttcggt aattttacct gaagattata
      961 atgctatttc tattatgacc atccacaaat cgaaaggttt acagtttcct attgtgatga
     1021 tgccgttttt aaactggagt gtaaagccaa aatcgaatga agtgatatgg gtgcaaacac
     1081 ccgatgagcc attcaataat tctatcattc cggtaaatat ttctgagaaa aaaatgttgc
     1141 tttcatcatt tgcctacgat tataatattg aaaaggataa taccattatc gattcgctta
     1201 atacccttta tgttgcattt acgcgtgccg aacaagcatt atttatttat actcagcaac
     1261 ttgccgaatc gccaacaatt aataaaatca atacgctcct ttccaatctc attaatattt
     1321 ctgaattgcg cgtccaccac caaagggctg ttgtccaact acagcacccg tgcatttatc
     1381 gttgatataa aaattaccgg cagcatgttc cagcttacgt gtcgcaagtt ctatagcata
     1441 acggtcgtgg gctatgattg cgcctgtgag ggcataggtt ccggtgcggt caacaaggtc
     1501 gagggtttct tcgtattttt tttcatcgta tacatatatg gttaatacag gtccaaaaat
     1561 ctcttcgcac attgttatgt agtcaggttt ttttgccagc aatatggtag gctcaataaa
     1621 atatcctttc gatttatcgt aattccctcc ggcaataatt tcaacatcct ta
//
LOCUS       DAORQA010000363         1667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_15075_length_1667_cov_3.068859, whole genome shotgun sequence.
ACCESSION   DAORQA010000363 DAORQA010000000
VERSION     DAORQA010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15075_length_1667_cov_3.068859"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /gene="ade"
                     /locus_tag="PK736_09915"
     CDS             complement(<1..1309)
                     /gene="ade"
                     /locus_tag="PK736_09915"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HRC33744.1"
                     /translation="MEVRGNIVDVINRRIYKGKIKYEGPKIINITEQENNEDVFIMPG
                     FVDAHIHVESSMLSPHYFSQEAVKHGTVATISDPHEIANVNGMQGVEYMLENAKTSAL
                     KIHFGAPSCVPATSFETAGAILDADAVQALLQRPDIWYLSEMMNYPGVLYNDAEVMAK
                     IKHAHKIGKPVDGHAPGLRGEQAAKYIAAGITTDHECFTLDEAIDKLKLQMKVLIREG
                     SAAKNFEALHPVISSYTDMVMLCSDDKHPDDLLLGHINKVAARAIASGHKLFDVLRCA
                     CVNPVLHYKVPVGLLRAGDAADFIVVSDLKNFDVIQTVINGNVVYKKNETQLVKAQQS
                     IINNFNTTYKNTNDFALEPYSDTQKKYYCIEALEGQLITNKLLFEPTVHQNNLVSNTT
                     TDILKIVVVNRYSNTKPAVAFIKNFGLKEGAIASTVAHDSHNII"
BASE COUNT          459 a          352 c          294 g          562 t
ORIGIN      
        1 caataatgtt gtgcgaatcg tgcgcaactg tcgatgctat tgcgccttct ttcaggccaa
       61 agttttttat aaacgccacg gcaggtttgg tgttgctata tctgttcacc acaacaattt
      121 ttaaaatatc ggttgtagta ttgctcacaa gattattttg atgtactgtt ggttcaaaca
      181 ataatttatt ggtaatcaat tgcccttcta atgcttcaat gcaataatat tttttttgcg
      241 tatcgctata aggttccaat gcaaagtcgt ttgtgttttt atatgttgta ttgaaattat
      301 ttatgatgct ttgttgtgct tttacaagtt gtgtttcatt ttttttgtaa accacatttc
      361 cattaatcac agtctgtatc acatcaaaat ttttcaaatc gctcactaca ataaaatctg
      421 cagcatcgcc cgcacgcaat aaacctaccg gcactttata atgcaacaca ggatttacgc
      481 aggcacatcg caacacatcg aacaacttgt ggcccgaagc aatggcacgt gccgccactt
      541 tatttatatg gcccagcaac aaatcatcgg ggtgcttatc gtcactgcat agcataacca
      601 tatctgtata cgatgaaatg acaggatgca gggcttcgaa attttttgcc gcactgcctt
      661 cgcgtatcaa cactttcatt tgcagtttca acttgtcgat ggcttcgtcc aatgtaaaac
      721 attcgtggtc ggtggtgatg cctgcggcaa tatattttgc agcttgctcg ccccgcaaac
      781 caggtgcatg gccatcaaca ggcttaccta ttttatgtgc atgttttatt ttcgccatca
      841 cctcggcatc gttgtacaat actccgggat agttcatcat ttcgctcagg taccaaatat
      901 cggggcgctg caaaagtgct tgtacagcat cggcatcgag tatagcacca gccgtttcga
      961 acgaagtggc cggcacacaa gatggcgcac caaaatgtat tttgagtgcc gaagtttttg
     1021 cattttccag catgtactcc acaccttgca ttccgtttac attcgctatt tcgtgtggat
     1081 cgcttatggt ggctactgtg ccgtgcttca ctgcctcttg tgaaaaataa tgaggtgaaa
     1141 gcatactgct ttctacatgg atatgcgcat ctacaaaacc gggcattata aaaacatcct
     1201 cgttattttc ttgttcagta atattaataa tttttggccc ttcgtatttt atcttgccct
     1261 tataaattct gcggttaatt acatcaacta tatttcctct tacttccatt gtgttttatt
     1321 tatttgcaaa taaagtgaaa aggaatggtt ggtgttatag aaaaatttag taggaatggt
     1381 tatatatctt tgccactgag gcaaaaagct tagccaaaag ttcttagcct atgctggtac
     1441 ggttttttaa aagcactcaa atagaacccc aataaaaaaa atcttcttta caaaacgaaa
     1501 acatttttta ctccaccacc accttaccca ccatcacatt attttgttca atcactttta
     1561 caaagtaaac tccttttgct gctttagata aatctacttg caaaatatta tcaataatat
     1621 tttccttttt gtaaattaat tcgcttgtag tggagtagat gtaaatg
//
LOCUS       DAORQA010000364         1661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_15128_length_1661_cov_7.177460, whole genome shotgun sequence.
ACCESSION   DAORQA010000364 DAORQA010000000
VERSION     DAORQA010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15128_length_1661_cov_7.177460"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..>1661)
                     /locus_tag="PK736_09920"
     CDS             complement(95..>1661)
                     /locus_tag="PK736_09920"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33745.1"
                     /translation="TYTVTVTDDCPVSQTLTIVISEPAMLMATCTVTNNVSCNGGNNG
                     FVNVAVVGGTPPYSTSPSTTFLTAGTYTFTVTDANGCSASCGATITQPAALMPNITTT
                     QSTCVNFPNGSATSSPSNGTPPYTYAWSNGSTDATVMNLTGGTYTVIVTDANGCSVIG
                     SATVTSVSVIPAQPGAISGNASVCLSSTGNVYSIVAVPGATSYTWSTGSTTATITSGQ
                     GSTSIMVSFSGTAVASTISVTANNVCGSSIARFRSYKVTAALKIPGPITGQTTGLCRK
                     INVPYSIGPVSGAISYFWTVPSGASIVSGQGTTAILVTYGNAFTGTGNVTVAAVNSCA
                     MGPVKSIFVNAKAAKPVIIGSNTTCMFQTGVVYSVAPVVGAASYTWTVVPGSSIVSGQ
                     GTTSIVVNWGNINGVISCKANNLCGSGLSGTLPVSFTCKEDGSAFDISELSLYPNPTD
                     NLATLDFDAFVDGNATLIITDMVGRVVSTENFDVTEGNNKRTIDVNQFAKGTYLVKVS
                     CEGSTRIVRLMVQ"
BASE COUNT          484 a          355 c          324 g          498 t
ORIGIN      
        1 ctttggatga atgaattcaa ttttaaaaca tagtaaaaca aaaaaagggg ctgcattgct
       61 acaacccctt ttaataaaat tatattctaa ttaactattg aaccatcaac cgaacaatac
      121 gcgttgaacc ttcgcacgaa acttttacaa ggtaagttcc ttttgcaaat tgattcacat
      181 caattgtgcg tttgttgttt ccttctgtta catcaaagtt ttctgtagaa actaccctgc
      241 ctaccatatc tgtaattatc aatgttgcat taccatcaac aaaggcatcg aagtctaacg
      301 ttgctaaatt gtcggtagga tttggataca atgataactc tgaaatatca aatgcacttc
      361 catcttcctt acatgtaaac gacacaggta atgtaccgct caatcctgaa ccacagagat
      421 tattggcctt gcaactgata actccgttta tattgcccca gtttacaaca atcgaggtgg
      481 ttccttgacc acttacaatt gagctaccag gtactactgt ccaggtatac gatgcagctc
      541 caaccacagg cgctacagaa tatacaactc ctgtttggaa catacatgta gtattcgagc
      601 ctatgattac tggcttggct gctttagcat ttacaaatat tgattttact ggacccatgg
      661 cgcatgaatt tactgctgcc acagttacat ttcctgttcc tgtaaaggca ttaccatagg
      721 taaccagtat ggctgtagtt ccttgtccag aaactataga cgctccactt ggcacagtcc
      781 agaaatatga aatcgcaccc gacacaggac caatactata tggaacattt atttttctgc
      841 ataatcccgt tgtttgtccg gtaataggac caggtatttt taatgcagct gttactttgt
      901 aagatctaaa tcgtgcaata cttgaaccgc aaacattatt tgctgttact gagattgtgc
      961 tggcaactgc ggtgccacta aagcttacca taatgcttgt gcttccttgc cctgatgtga
     1021 tagttgcagt ggttgaacct gttgaccacg tatatgatgt agccccgggt actgcaacta
     1081 tgctgtaaac attacctgtt gaacttaagc aaaccgatgc attgccgcta atagcaccag
     1141 gctgtgcagg aatcacagat acagaagtta ctgttgctga gccaattaca gaacagccat
     1201 tagcatcggt aacaattact gtatacgtac ctcctgtcag attcattact gttgcatcgg
     1261 tacttccatt gctccatgca taagtatacg gaggggtgcc attgcttggg cttgatgttg
     1321 cacttccatt tgggaaatta acacaagttg actgtgttgt agtgatattt ggcatcaaag
     1381 cagcaggttg tgttatggta gcaccgcatg aagcagaaca tccattagca tctgtcacag
     1441 taaaggtata tgttccggca gtcaggaatg ttgtggatgg tgacgtagag taaggtggag
     1501 tacctccaac aactgcaaca tttacgaaac cattatttcc accattacat gaaacattat
     1561 ttgtaactgt acatgtggcc attagcatcg cgggttcact aattacaatg gtgagtgttt
     1621 gtgaaacagg gcaatcatca gtaacagtaa cagtatacgt t
//
LOCUS       DAORQA010000365         1659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_15159_length_1659_cov_4.650249, whole genome shotgun sequence.
ACCESSION   DAORQA010000365 DAORQA010000000
VERSION     DAORQA010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15159_length_1659_cov_4.650249"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="PK736_09925"
     CDS             <1..1145
                     /locus_tag="PK736_09925"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008504277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC33746.1"
                     /translation="VNGVQALIVVPSRELALQIEQVFKQMGTTYKVNCCYGGHATRTE
                     KNNLIQPPAVLIGTPGRLAFHIRNENIDTSSIHFLVLDEFDKAMEYGFQDDMTFIVNS
                     LRKLKKRMLTSATGMKEIPKFARLNNAIEINYLSHTPVQAEGLKLKVVKATTKDKIDS
                     LFSLICKLGNKATLIFCNHRDAVDRISELLNRKGLIHGTFHGKMEQEDRERALIKFRN
                     GTYQILITTDLASRGLDIPEIENIIHFQLPATEDILIHRNGRTARMNATGTAYIIIGP
                     DEHLPKFIKEIPEEETMPSKNKIPDNTPWCTLYIGAGKKDKINKMDIVGMLLQKGKLH
                     KDDLGLIEVLDFSAYAAVKRNALTGVLKLIKDEKIKNRKVKIEESK"
     gene            complement(1160..>1659)
                     /locus_tag="PK736_09930"
     CDS             complement(1160..>1659)
                     /locus_tag="PK736_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRC33747.1"
                     /translation="NIIDYINHPQRIFPIGRLDKDSEGLIFLTNDGDIVNKILRAGNN
                     HEKEYIVTVDKSLTKTFLNDMVSGVKIMETITQKCFVEQEGRNRFRIILTQGLNRQIR
                     RMCEALDYKVVKLKRTRIMNINLNGLNYGQWRYLNDDEVKAINDLVATSSKTEEASVL
                     KEIVD"
BASE COUNT          553 a          304 c          312 g          490 t
ORIGIN      
        1 atgtaaatgg tgtgcaggca ttgatagtgg ttccatcgcg cgagttggca ttgcaaatag
       61 agcaggtgtt taaacaaatg ggcactacct acaaagtaaa ttgctgctat ggtggacatg
      121 caacccgcac cgaaaaaaat aatttgatac aaccaccggc agtgttgata ggtactcccg
      181 gccgtttggc ttttcacatt cgaaatgaaa atattgacac atcatccatc cattttctgg
      241 tgcttgacga atttgataaa gcaatggaat atggttttca agatgacatg acttttatag
      301 tgaacagctt gcgaaaatta aaaaagcgaa tgcttacctc tgcaaccgga atgaaagaga
      361 ttccaaaatt cgccagatta aataatgcga tagaaataaa ttatttgagc cacacgcctg
      421 tgcaagccga aggtttgaaa ctgaaagtag taaaagcaac tacgaaagat aaaattgatt
      481 cgttgttttc gttgatttgt aaattaggaa ataaggcgac acttattttt tgcaatcatc
      541 gcgatgctgt ggatcgtata agcgaattgc taaaccgaaa aggattgata catggaacat
      601 ttcatggtaa aatggaacag gaagacagag agcgtgcatt gataaaattt agaaatggca
      661 cgtaccaaat tttaatcacc accgaccttg catcgcgcgg gctcgacatt cccgaaatag
      721 aaaacattat tcattttcaa ttgcctgcaa ccgaagatat acttatacac cgcaatggcc
      781 gcactgcgcg catgaatgca acaggtacag cttacattat tattggcccc gatgaacatt
      841 tgccaaaatt tataaaggaa attcccgagg aagaaactat gccatcgaaa aataaaattc
      901 ccgacaatac tccttggtgt accttataca ttggtgccgg taaaaaagat aaaataaaca
      961 agatggatat tgttggcatg ctgctgcaaa aaggaaaatt gcataaagac gatttgggct
     1021 tgattgaagt gttggatttt tcggcttatg cagcggtgaa aagaaatgcg ttaaccggtg
     1081 tattgaaatt aattaaagac gaaaaaataa aaaacaggaa agtaaagatc gaagaatcga
     1141 aatgatgttg acacaatatc taatcaacaa tttctttcaa taccgaagct tcttctgttt
     1201 tacttgatgt ggctactaaa tcatttatcg cttttacttc atcatcatta agatatcgcc
     1261 actggccata atttaatccg tttaaattaa tattcattat tcgagtgcgt tttagtttta
     1321 caactttata gtcaagggcc tcacacattc ttcgtatttg ccggtttaat ccctgagtta
     1381 aaataatccg aaaccggttt ctaccttctt gttcaacaaa acacttttgt gtgatggttt
     1441 ccataatttt tactccgctt accatatcat tcaaaaatgt tttggtcaac gatttatcta
     1501 ccgtaacaat atattctttt tcatgattgt tgccggcccg caaaatctta tttactatat
     1561 cgccatcgtt tgtcaaaaat attaaccctt ccgaatcttt atcgaggcgg ccaatcggga
     1621 aaatacgctg aggatgattg atgtaatcaa taatattat
//
LOCUS       DAORQA010000366         1651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_15236_length_1651_cov_7.387218, whole genome shotgun sequence.
ACCESSION   DAORQA010000366 DAORQA010000000
VERSION     DAORQA010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15236_length_1651_cov_7.387218"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PK736_09935"
     CDS             complement(<1..911)
                     /locus_tag="PK736_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33748.1"
                     /translation="MYIVHSVFYFWHVRLKTIIYTLSIIAFIIVAGFFLCRNYLLHIA
                     FEKIQTKLTKEVNLNLTAKEINFQGLFRVEASDVNLYAEGADTLATINKAACDIGFLT
                     LIKGRIGLDFLSLKGVNIQLIDSVGHNNYKSLLLRKGKVIDNDTIIATTGYQEQMTTL
                     FDRVFKLLETSFELNDISIKYSSEKENQCFTIPSARYDLRALNAIIYASGLNQTDTFK
                     IKFNVVENRKKYMAEFNNITSMQHYLPFFSSDSSLKIKLQNMVCHFNIEKNYSNKMVM
                     NCDLQIKNLSLHHFQISPDDITIYDAGV"
     gene            complement(941..>1651)
                     /gene="cas6"
                     /locus_tag="PK736_09940"
     CDS             complement(941..>1651)
                     /gene="cas6"
                     /locus_tag="PK736_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HRC33749.1"
                     /translation="DQNSSNSIPLHHQKLLSSALHELVSSFALEPLPFNFSSIKGTSR
                     IQNGYMRFLSTKVTMVVSAADADYCNKIVDEIFSRPYLTIGKLNIMPRFKEIIADPPI
                     TKRMRYICISPLILFDPKIDQAKAQELINPNTKEFADALYNVVMDQMEKAGYTEEQLA
                     QYAEFESQPDKEYVEKVQDSGKKFARFYKCSEGFTMLGYLLPFTLYAHPDVHDFIWKN
                     GIGVLNNEGYGMVDVVTN"
BASE COUNT          486 a          279 c          318 g          568 t
ORIGIN      
        1 actccggcat cgtaaatggt tatgtcatcg ggcgaaattt ggaaatgatg gagtgaaaga
       61 tttttgattt gtaaatcgca attcattacc attttgttac tgtagttttt ctcaatattg
      121 aaatggcaaa ccatgttttg tagttttatt tttaatgaac tatcgctact aaaaaatggt
      181 aaataatgtt gcatgcttgt tatgttatta aattctgcca tatacttttt tcgattttca
      241 accacgttga attttatttt gaaagtatct gtttggttta aaccggaagc ataaattatg
      301 gcattgagtg ctcttaaatc atacctggca gatggaattg taaagcattg gttttccttc
      361 tcgcttgaat attttataga aatatcgtta agttcaaagc tggtttcgag cagtttaaaa
      421 accctgtcga atagggtcgt catttgttct tgatatccgg ttgttgcaat gattgtgtcg
      481 ttatctatca ctttgccttt tctaagcaac agcgatttgt aattattatg gcccacggaa
      541 tcaatcaatt gaatatttac acccttgagg cttaagaaat cgaggcctat cctacctttt
      601 attagtgtaa ggaaacctat atcgcaagct gctttattta tggtggcaag tgtatccgca
      661 ccttcggcat ataaattcac atcagatgct tcaacacgaa ataatccttg aaaattaatt
      721 tctttggctg taagattgag gtttacttct ttggtaagtt tggtttgtat tttttcgaat
      781 gctatgtgca gtaagtaatt gcggcataag aaaaagcctg caacaatgat gaaggctatt
      841 attgataaag tatagatgat ggtcttgagt cgcacatgcc aaaaataaaa aacagaatgc
      901 acaatgtaca ttctgtttca aaataaatat ttattatgta ttagttagtt acaacatcta
      961 ccataccata accttcgttg tttaacacac caataccgtt tttccatata aaatcatgca
     1021 catcgggatg ggcgtacaaa gtaaatggaa gcagatagcc tagcattgta aaaccttcgc
     1081 tgcacttata aaagcgggca aactttttac cggagtcctg cactttctcc acatattcct
     1141 tatcgggttg cgattcgaat tcggcatatt gtgcaagttg ttcttcggtg tagccggctt
     1201 tttccatttg atccattacc acattgtaaa gtgcatcggc aaattctttt gtgtttggat
     1261 ttattaattc ctgggctttg gcctgatcaa tctttggatc gaaaagaata agtggcgaaa
     1321 tgcatatata acgcatacgc ttagttatag gcgggtcagc aattatttct ttgaaccgcg
     1381 gcataatatt cagcttgcca attgttaagt atggtcggct aaaaatttcg tctactatct
     1441 tattgcaata gtctgcatcg gctgcagata ctaccatagt aacttttgtt gacaagaacc
     1501 tcatgtagcc gttttgaata cgtgatgtgc ctttaataga ggaaaaattg aaaggcaaag
     1561 gttccaaagc gaacgaactt acaagttcat gtaatgctga tgataacagc ttttgatggt
     1621 gcaagggaat agagttagat gagttctgat c
//
LOCUS       DAORQA010000367         1651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_15237_length_1651_cov_6.960526, whole genome shotgun sequence.
ACCESSION   DAORQA010000367 DAORQA010000000
VERSION     DAORQA010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15237_length_1651_cov_6.960526"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1651)
                     /locus_tag="PK736_09945"
     CDS             complement(<1..>1651)
                     /locus_tag="PK736_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRC33750.1"
                     /translation="DVFTKDLLISSVHIRESDPEKAQLYANQSLALAQKNSNKKGQAD
                     AYHNMAAIQYNNGDYDKSLELYNLSLTLRNQIGNKKDIAGSYLNIALVYWDKGNFPEA
                     LSNNFKALKLYETMNDKTLTAGCYNNLGMIYDDQGNQAQALKNYLFALKLFEDANDAK
                     GISIATFNIGGIYDSKGAFDKALENYTRSLKLNEEANDKKNMAQCFNNIGETNAKLKL
                     FDKALENFNRALKLNNEINNRNSITYNKLNIARTYIEQKKYSAAFPFLTDALSMAQEL
                     DDADNIMECHHAMSILDSAKGNFAQSMEHYKLYIDYRDKLFNETNTKKLVETQMLYEF
                     DKKESFAKAQQEKKDAVALKELQRQKIVRNSFVGGFVFVSLFAGIFFSQRNRIRKEKK
                     RSEDLLLNILPEEVAQELKDKGSADAKQFDEVTVMFTDFKGFTQIAEKLSAKELVAEI
                     DTCFKAFDNIITKYNIEKIKTIGDAYMCAGGLPVANKTHAEDVVKAAIEILQFMEGHL
                     KASPLGRFGGARIGIHTGPVVAGIVGIKKFAYDIWGDTVNIA"
BASE COUNT          429 a          315 c          296 g          611 t
ORIGIN      
        1 gcaatgttca ctgtatcgcc ccatatatcg tatgcgaatt ttttaatgcc gacaattcct
       61 gcaacaacgg gtccggtatg aatgcctatt cgcgcccctc caaacctccc caaaggggag
      121 gcttttaaat gcccctccat aaattgaaga atttcaattg ctgctttcac aacatcttcg
      181 gcatgtgttt tatttgcaac aggcaagcca cctgcacaca tgtatgcatc gccaattgtt
      241 ttaatttttt cgatgttgta ctttgtgata atgttgtcaa atgctttgaa gcaagtatca
      301 atttcggcaa ccaattcttt ggcagaaagt ttttcggcaa tttgtgtaaa acctttgaag
      361 tcggtaaaca ttacagttac ctcatcaaat tgttttgcat cggcactgcc tttatctttt
      421 agttcttgtg ctacttcttc gggcaggata tttaagagta aatcttcgct gcgttttttt
      481 tcttttctaa ttctattgcg ttgactgaaa aatattccgg caaaaagcga aacaaataca
      541 aatcctccca caaaactatt tcgcactatt ttttgccgct gtaattcttt caaagccacg
      601 gcatcttttt tttcctgctg tgcttttgca aaagattctt ttttgtcgaa ctcatatagc
      661 atttgtgttt ccactaattt cttagtgttt gtttcattaa acagcttatc acgataatct
      721 atataaagtt tataatgttc catcgactgt gcaaagtttc ctttcgcact atcgagtatg
      781 gacattgcat gatggcattc cataatgttg tcggcatcgt caagttcctg tgccattgac
      841 aatgcatcag taagaaaagg aaatgcagca ctatattttt tttgttcgat ataggtgcgt
      901 gcaatattta gtttattata ggtgatggag tttctattat ttatttcgtt gtttaatttt
      961 aatgcacggt taaaattttc aagagccttg tcaaagagtt ttaattttgc atttgtttct
     1021 ccaatattat taaaacactg agccatgttt tttttatcgt tagcttcttc attaagtttt
     1081 agagagcgtg tatagttttc aagtgctttg tcaaacgctc cctttgagtc gtatattcca
     1141 cctatattaa aagttgcaat cgaaattcct ttagcatcgt tcgcatcttc aaatagtttc
     1201 aatgcaaaca aataattttt taaagcctgt gcctgattac cctgatcatc gtatatcatg
     1261 cccaggttat tgtaacaacc ggcagtgagg gttttatcat tcatggtttc gtacaacttt
     1321 agtgctttaa aattatttga aagtgcttca ggaaagtttc ctttatccca atacactaaa
     1381 gcaatgttga ggtacgaacc cgcaatatct tttttgttgc caatttgatt gcgtaatgtg
     1441 agcgacaggt tgtaaagttc caatgattta tcgtaatcgc cattgttgta ttgtatggcg
     1501 gccatattgt ggtaggcatc ggcctggcct tttttgttgc tgtttttttg tgccaaagcc
     1561 aatgattgat tggcatatag ctgtgctttc tcggggtcgc tttcgcgaat gtgtaccgaa
     1621 gagataagta aatcttttgt gaaaacatct t
//
LOCUS       DAORQA010000368         1627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_15486_length_1627_cov_5.160941, whole genome shotgun sequence.
ACCESSION   DAORQA010000368 DAORQA010000000
VERSION     DAORQA010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15486_length_1627_cov_5.160941"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..639)
                     /locus_tag="PK736_09950"
     CDS             complement(208..639)
                     /locus_tag="PK736_09950"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HRC33751.1"
                     /translation="MKYYFALLISLVFISCNNKPQPIKYDMDECIECKMTIVDKKYGA
                     EIISTKGKIYKFDDVICMTDFMNASKSEENDIKKILVINFEKENDFLDVNQASFYVSD
                     DLHSPMNGNAAAFVNAQQAILFQAGKQGIIMDWKELKENLK"
     gene            complement(645..1220)
                     /locus_tag="PK736_09955"
     CDS             complement(645..1220)
                     /locus_tag="PK736_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33752.1"
                     /translation="MKKLHILSRILIGIASLTIIIAYFFPVWRIDLFAPQYPEGLVIK
                     IWLNNITGDVDVVNGLNHYIGMKKISVEMFPEFSFLIYIVALFICYGLTIAFTGSRKL
                     LFSYLVVSAIGGVAALYDFYLWGYDYGHNLNPDAPIKVPGMGYQPPVIGHKQLLNFDA
                     YSMPDVGGYIVIVAVVVAGIIWIVEKRLDKK"
     gene            complement(1280..>1627)
                     /locus_tag="PK736_09960"
     CDS             complement(1280..>1627)
                     /locus_tag="PK736_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019991170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRC33753.1"
                     /translation="EYVNDIANAGPFTVFAPTNAAFEKLPAGTVESLMKPENKDKLRE
                     ILEYHVSVGGYKQDYLTDGQTINQANLKNITINIKDGKMMVNGTVNVVATVPASNGIV
                     YVIDQVLLPPAEK"
BASE COUNT          496 a          314 c          267 g          550 t
ORIGIN      
        1 actaatagca gatgcagacc agtcactcat ttgttcaatc actttgtcaa tcactccctt
       61 ttctataaaa acagtatcgt tttcatttgc atcctttatt gcttttgtga ttgttttgta
      121 tgcacgattt gcacctacat aaatatgccc tgcataaata agttagatgc aaaaattaaa
      181 aatatggtta atgaggtgcg cacaatttta cttaaggttt tcttttaatt ctttccaatc
      241 cataataatc ccttgcttgc ctgcttgaaa tagaatagct tgttgtgcat tcacaaacgc
      301 tgcagcattg ccattcatgg gcgaatgtaa atcgtcactt acgtaaaacg aggcttgatt
      361 cacatcaaga aaatcatttt ctttttcgaa attgattacg agtatttttt taatatcgtt
      421 ttcttctgac ttagatgcat tcataaaatc agtcatgcaa attacatcgt caaacttata
      481 tatttttcct ttggtggaaa taatttcggc tccatatttt ttatccacaa tagtcatttt
      541 gcactcaata cattcatcca tgtcgtactt aatgggttgt ggtttgttgt tgcatgaaat
      601 aaacacaagc gaaattaata aggcgaaata gtatttcatt ttttttattt tttatccaaa
      661 cgtttttcta ctatccaaat aatgccggca acaaccacag caactataac tatataccca
      721 cctacatcgg gcatcgaata tgcatcgaag ttgagcagct gtttgtggcc tattaccgga
      781 ggctgataac ccatgccggg aactttaatt ggtgcatcgg gatttaagtt atgcccataa
      841 tcgtaacccc ataaatagaa atcgtatagg gcggcaacac caccaatggc cgataccaca
      901 agatagctga ataataattt gcggctgccg gtaaaagcaa ttgttaaccc atagcaaata
      961 aacaaggcaa caatgtaaat taaaaacgaa aattcgggaa acatctcgac acttattttt
     1021 ttcatgccta tgtagtggtt caggccattt acaacatcaa cgtcaccggt aatattattc
     1081 agccatattt ttattactaa tccttcggga tactgtggtg caaataaatc gatgcgccat
     1141 actggaaaaa aataggcgat aattattgtt agtgaagcaa taccaattaa tatcctagag
     1201 aggatgtgaa gttttttcat tttgttttta tttaatttaa aaaccccttt acaatgaaag
     1261 gggtttttaa aaatatattt tatttctcgg ctggaggcag cagcacctga tctatcacat
     1321 atacaattcc atttgaagcc ggaactgtag caacaacatt tactgttcca ttcaccatca
     1381 tcttaccatc tttaatattg atggtaatgt tttttaaatt ggcttgattt attgtttggc
     1441 catcggtaag ataatcttgt ttgtatccac caaccgaaac gtggtactct aaaatttcgc
     1501 gcagcttatc tttattctca ggcttcatta aactttctac agtgcctgca ggtaattttt
     1561 cgaaggctgc attggttggg gcaaacaccg tgaacggtcc ggcattggca atatcattta
     1621 catattc
//
LOCUS       DAORQA010000369         1617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_15593_length_1617_cov_5.886684, whole genome shotgun sequence.
ACCESSION   DAORQA010000369 DAORQA010000000
VERSION     DAORQA010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15593_length_1617_cov_5.886684"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="PK736_09965"
     CDS             <1..1142
                     /locus_tag="PK736_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC33754.1"
                     /translation="DTLNEILSQPEFKKHLQGILDKNQIRINTNDDTKMFLPSALKKN
                     NHPLFEKIKTAYNNFVALILEKHQVDKTQPEIQKILNQIANPDVSNNTDGIPEDDTLE
                     KVLAELQAMIGLEEVKKDINDLVNLIKVQKTREQQGLKNPDTSLHIVFLGPPGTGKTS
                     VARLLGRIYKHLGMLDKGHLVETDREGMVAGYVGQTAIKVDEIVERSIEGVLFIDEAY
                     SLSQNVMGNDFGKEAIDALVKRMEDNRKDLAVIMAGYTEPMKELIESNPGLRSRFNRY
                     FKFDHFTPEQLLEIFKTFCNKSDFIIDDNASEKLHDTFKLLYEKRDEGFGNARVVRNL
                     FEKCVQSHANRIVSIKEQSPEVLKTLTEADIPEPKKTVAQVYFSV"
     gene            complement(1254..>1617)
                     /locus_tag="PK736_09970"
     CDS             complement(1254..>1617)
                     /locus_tag="PK736_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33755.1"
                     /translation="YTAMDNNVGYKKDIDAYTAYFKKQFAMQSDVIGFIAVSGDKILG
                     CDLFATNALFTQQLDNLINSFATEAITSGKKPAVAYNKIIAYYNDFLADETKQDEKIK
                     NNGTQLKIKQTKLHIAHY"
BASE COUNT          540 a          282 c          308 g          487 t
ORIGIN      
        1 tcgatacact gaacgaaatt ttatcgcagc ccgaattcaa aaaacatttg cagggaatat
       61 tggataaaaa tcaaatcagg ataaatacca atgacgatac aaaaatgttt ttgccatcgg
      121 cattgaaaaa aaataatcat ccgctattcg aaaaaataaa aaccgcttac aataattttg
      181 ttgccttgat tttagaaaaa catcaggtag ataaaacaca accagaaatt caaaaaatat
      241 taaaccaaat tgcaaacccc gatgtaagca ataatacgga tggtataccc gaagatgaca
      301 cacttgaaaa agtgttggcc gaattgcaag ccatgattgg tttagaagaa gtgaaaaagg
      361 atatcaacga tttagtgaat cttataaagg ttcaaaaaac acgcgagcaa caaggcttaa
      421 aaaatcctga cacttcttta catattgttt ttttaggacc tcccggcacc ggcaagactt
      481 ctgttgcacg tttgcttggc cgcatataca aacacctggg aatgcttgat aaaggccatc
      541 ttgtagaaac agatcgcgaa ggaatggtgg caggttatgt tggacaaact gctattaaag
      601 ttgacgaaat agtggagcga agtatagaag gtgttttgtt tattgatgag gcttattctc
      661 tttcgcaaaa tgtgatgggc aacgattttg gcaaggaagc aattgatgca cttgtaaaac
      721 gtatggaaga taaccgcaag gacctcgcag taattatggc cggctatacc gaaccaatga
      781 aagaattaat cgaaagcaat cctggtttac gttcgcgctt caaccggtat tttaagttcg
      841 accattttac tcccgaacag ttgctcgaaa tttttaaaac gttttgcaac aaatccgatt
      901 ttataattga tgataatgct tccgaaaaac tccacgatac atttaaactg ctttacgaaa
      961 agcgcgatga aggatttggt aatgcacgtg tggtgcgcaa cctgtttgaa aaatgtgtac
     1021 aaagccatgc caacagaatt gtatcgataa aagaacaatc gcccgaagta ttaaaaaccc
     1081 tcaccgaagc cgatattccc gaacctaaaa aaacagtggc gcaggtttat ttttcggtgt
     1141 aagaagtttt gcaatgttga tgcgtttttc ttaaaacaat ctaacaatcc caacttttga
     1201 atttaactga ttgctgttat aaaagtaaaa caatatacct tgcagtctgt gcatcaataa
     1261 tgagcaatat gcaatttggt ttgttttatt tttaattggg ttccgttgtt ttttattttt
     1321 tcgtcttgct tagtttcatc agctaaaaaa tcgttgtagt atgctattat cttgttgtat
     1381 gcaacggctg gtttttttcc tgaggtaatt gcttcggtag cgaaagagtt tataagatta
     1441 tcgagttgct gtgtaaacaa agcgttggtg gcaaaaaggt cgcagcctaa tattttatcg
     1501 cccgatacag ctataaaacc tatgacatct gattgcattg caaattgttt tttaaagtaa
     1561 gcagtgtatg catctatatc ttttttatat ccaacattat tatccattgc tgtatac
//
LOCUS       DAORQA010000370         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_15610_length_1616_cov_3.714286, whole genome shotgun sequence.
ACCESSION   DAORQA010000370 DAORQA010000000
VERSION     DAORQA010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15610_length_1616_cov_3.714286"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PK736_09975"
     CDS             <1..271
                     /locus_tag="PK736_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRC33756.1"
                     /translation="DYTILEELVKDKVKAIVCLGTDNKKIIKAFGKYVDIIVETASAE
                     EAVRQANHISKKGDVVLLSPACASFDLFKNYEERGRLFKAAVKSL"
     gene            282..1436
                     /locus_tag="PK736_09980"
     CDS             282..1436
                     /locus_tag="PK736_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008203145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRC33757.1"
                     /translation="MVKEWIENNLRGDKMIWFIVAVLSVFSLLAVYSSTGTLAYKYQS
                     GNTEYYFLKHFFLLALGLVIMFFAHKITYTAYSKVARVGLFVVIPMLLFTLLGGTNLN
                     DASRWLTLPVVGLTFQTSDFAKLALILFVARALSRKQDDIANFKEAFLPIIVPVVVVC
                     GLIFPANFSTAAILFTTCLFLMFIGRVRAGFIFGLLGIGVVALALFLGIAKLVNYEGR
                     ISTWTNRIENFRNGDSEDNYQAEQAKIAIAKGGVMGLGPGNSETRNFLPHPYSDSIFP
                     IIAEEYGLIGPFIVLLMYILLYYRAVVMVRKSPKAFATLLAFGCSFSLVFQAMINMAV
                     ATNIFPVTGQPLPLVSMGGTSMWFTSLSIGILLSVSHAIQKEGGEKVATV"
     gene            1423..>1616
                     /locus_tag="PK736_09985"
     CDS             1423..>1616
                     /locus_tag="PK736_09985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC33758.1"
                     /translation="MQQSNYKIIISGGGTGGHIFPAIAIAHALKAINQRHDILFVGAN
                     GRMEMEKVPAAGFKIIGLNIA"
BASE COUNT          464 a          294 c          354 g          504 t
ORIGIN      
        1 cgattatacc attctcgaag aattggtaaa agataaagta aaagcaattg tttgcttagg
       61 caccgataat aaaaaaataa taaaagcctt tggaaaatat gtggatataa ttgtagaaac
      121 agcatcggcc gaagaggctg tgagacaagc caaccatatt tcgaaaaagg gggatgtggt
      181 tttattatcg cctgcatgtg caagtttcga tttgtttaaa aattacgaag agcgaggccg
      241 cttattcaaa gctgcagtaa aatcattata atataattgt catggtaaaa gagtggatag
      301 aaaataatct tcgtggcgat aaaatgattt ggttcattgt agccgtgctt tctgtatttt
      361 cgttgttagc cgtatattcc tctacgggta cactggctta taaatatcaa agcggaaaca
      421 cagaatatta ttttctaaaa catttcttct tgcttgcatt aggtttggtg ataatgtttt
      481 ttgctcacaa aataacttat actgcctatt cgaaagtggc gcgtgttggt ttgtttgtag
      541 taattccaat gttgctgttc actttgcttg gtggcacaaa cctcaatgat gcaagccgtt
      601 ggttaacgct gccggtggta ggattaactt ttcaaacctc cgactttgca aagctggcat
      661 tgattctttt tgttgcccgt gcactatcgc gtaagcaaga tgatattgca aattttaaag
      721 aagcctttct tcctattatt gtaccggtag tagttgtttg cggattaata tttccggcaa
      781 acttttctac agcagccata ttatttacca catgtttatt cctaatgttt attggccggg
      841 tgcgtgcagg ttttattttc ggattgttag gcattggagt tgttgcactc gctttgtttt
      901 taggaatagc aaaattggta aactacgaag gacgtatatc cacatggact aaccgcatcg
      961 aaaatttccg gaatggcgat agtgaagata actatcaggc agagcaagca aaaatagcca
     1021 ttgccaaagg cggagtaatg ggccttggac ccggcaacag cgaaacccgc aactttctgc
     1081 ctcatcctta ttccgattct atatttccaa ttattgccga ggaatatggt ttgatagggc
     1141 cattcattgt tttactaatg tacatattac tctattaccg ggcagtggtg atggtccgaa
     1201 aaagccccaa agcatttgct acactccttg cgtttggttg tagtttcagt ctagtgtttc
     1261 aggcaatgat aaacatggcc gtagctacaa atatatttcc tgtgaccgga caacctttgc
     1321 cacttgtaag catgggagga acttctatgt ggtttacaag tttatctatc ggtatattat
     1381 taagtgtgag tcacgcaata caaaaagaag gaggcgaaaa agttgcaaca gtctaactat
     1441 aaaataatca tcagtggtgg tggcaccgga gggcatatct ttccggccat tgctattgct
     1501 catgcgctga aggctatcaa tcagcgccat gatattttat ttgttggcgc caacggccga
     1561 atggaaatgg agaaagtgcc ggccgcaggt ttcaaaatta ttggactcaa cattgc
//
LOCUS       DAORQA010000371         1609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_15671_length_1609_cov_4.959459, whole genome shotgun sequence.
ACCESSION   DAORQA010000371 DAORQA010000000
VERSION     DAORQA010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15671_length_1609_cov_4.959459"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>595
                     /locus_tag="PK736_09990"
     CDS             <1..>595
                     /locus_tag="PK736_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33759.1"
                     /translation="QYTGGKYKLFAEALSNFELSDSTEVNLFTPIEEPKKDSVKTETK
                     STGEIIKTEPVEKKDSSKVDINNYTFQSEFSKKKKKKDAAFVVDSSAVTIDSTKTSTS
                     STTAITPEPYVMTSGDTIYIFPKQRNYDVAFNSQYFVSQLDNQLLNATYQTFTGGSPF
                     FDPGLNGLFKIGLTDLMEDYRITAGIRLAGNFNSNEYI"
     assembly_gap    596..634
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <635..1592
                     /locus_tag="PK736_09995"
     CDS             <635..1592
                     /locus_tag="PK736_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33760.1"
                     /translation="NRIDQSVMVYRQSRQDFTQFSLLKITTHEAKYINKYPLNDLTAV
                     RATVSLRNDRLVALSTDYFNLAEKNQDKYWGSGKVEYVYDNTIATGLNLKNGFRYKFF
                     AEVFDQISEKKNLLIVFGFDARHYLKVYKQMIWANRIAGSTGAGDAKLIYYLGGVDNA
                     ITPVVNFDNTIRIDNTKNYVFQTLATNMRGFKQNIRNGSSFTLWNSELRIPIFQVLSG
                     RPLRSDFFRSFQLIAFTDVGTAWSGTSPWSPDNNLNLDIIQTGPLTITVKKNTDPIVA
                     GFGWGMHARVLGYFMRADWGYGYADNVLAKKPLFNFSLGLDF"
BASE COUNT          493 a          303 c          316 g          458 t
ORIGIN      
        1 caatacaccg gaggcaaata taaactattt gccgaagcgc tttccaactt tgaattgtcg
       61 gatagtactg aagtaaattt atttacccca atcgaagagc ctaaaaaaga tagtgtcaaa
      121 accgaaacaa aaagcacagg agaaattata aaaactgaac ctgtagagaa aaaagattct
      181 tctaaagttg atatcaataa ttatacgttt caatcggagt ttagcaaaaa gaaaaaaaag
      241 aaagatgcag cctttgtggt tgatagttca gctgtaacaa ttgactcaac taaaacttct
      301 acatcatcca caactgccat tacacctgag ccttacgtga tgacatcggg cgataccatt
      361 tacatttttc ccaaacaacg aaactatgat gtggcattca actcgcagta ttttgtaagc
      421 caactcgaca atcaattgtt gaatgcaacg tatcaaactt ttaccggagg ctcaccattt
      481 ttcgatccgg gtttgaatgg tttgttcaaa ataggattga cagatttgat ggaagactat
      541 cgcataacag ccggcataag gcttgcaggg aatttcaaca gcaacgaata tattcnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncaatcg cattgatcaa tcggtgatgg
      661 tttacagaca atcgcgccag gattttacgc aattttcctt gttaaaaatt acaacacacg
      721 aagcaaaata cattaacaaa tatccgctga atgatttaac cgctgtgcgg gctacagtat
      781 ctttgcgcaa cgataggctg gtagctttga gcaccgacta ttttaacctt gccgaaaaaa
      841 atcaggacaa atactggggc tcgggcaagg tggagtatgt gtatgataat actattgcaa
      901 ccggcctgaa tttaaaaaat ggttttcgtt ataagttttt tgccgaagta ttcgatcaga
      961 taagcgagaa gaaaaacctt cttattgttt ttggtttcga tgcacgccat tatcttaaag
     1021 tgtataagca aatgatatgg gctaaccgca tagcaggtag cacaggtgcg ggcgatgcca
     1081 aactcattta ctatttgggt ggtgttgata atgctatcac tccggttgtg aatttcgaca
     1141 ataccattcg gattgataat acaaaaaatt atgtatttca aactttggca accaatatgc
     1201 ggggcttcaa acaaaatatt cgcaatggaa gttctttcac tctttggaac agtgaattgc
     1261 gtattcccat atttcaagtg ttgagcggaa ggccgctgcg ttccgatttt ttccgtagtt
     1321 ttcaacttat agcattcacc gatgtgggca ctgcatggtc gggtacttca ccttggtcgc
     1381 ccgacaataa tctcaatctc gatattattc aaacaggacc tctcaccatt acggtaaaga
     1441 aaaataccga ccctattgtt gcaggatttg gatggggaat gcatgcacgt gtattaggat
     1501 actttatgcg tgccgactgg ggctatggtt atgccgacaa tgtgctggcg aaaaaacctt
     1561 tgttcaattt ttcgctcggc ttagattttt aattttatta tttttaacg
//
LOCUS       DAORQA010000372         1599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_15792_length_1599_cov_3.987694, whole genome shotgun sequence.
ACCESSION   DAORQA010000372 DAORQA010000000
VERSION     DAORQA010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15792_length_1599_cov_3.987694"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PK736_10000"
     CDS             <1..448
                     /locus_tag="PK736_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRC33761.1"
                     /translation="MIPLTDVLSINDEEARLLSKEYSLRKAAKIIMAMGPKTLIIKKG
                     EHGALLFNGNNVFFAPALPLEEVFDPTGAGDTFAGGFIGYLAKTSDISFDNMKRAIIF
                     GSAMASFCVEKFGPERLLSLTDKEITARVNDFYDLVEFDLIDALNI"
     gene            complement(465..>1599)
                     /locus_tag="PK736_10005"
     CDS             complement(465..>1599)
                     /locus_tag="PK736_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33762.1"
                     /translation="TKTVTSNDFPNDGAIVLSDDITHDTILINHKQGVDYRIAICMKV
                     TNGARLIIQPGVTIEMTPGSGIEISDAASIDWQGNANEKINILGLEKTRGSWRGIYLY
                     SKGVTQIMKHCIIQHAGSDIYKIEKRSCINVLGASVSMENCTIKDNFANGLYLDSAAV
                     NIGIHNNTFTGNYAEPVMIDHVNCDILTNDNLYTGNTFDRIYIRTIRNLQTYRTLNIP
                     KLDVPYTVDSTFNIGTTVIIQPGTKFIMQKLSLFYANDSLQNGASFTAIGTTNNRIEF
                     VGQLGKKGYWQGIGLDIGATCHFENCDIKYCGFANNYSSSNPEAMIIIGGNTNTNCEI
                     RNCIFSYSAANGIAIDSPSVNFNADIYTVNSFDSIDFANVLIY"
BASE COUNT          476 a          301 c          297 g          525 t
ORIGIN      
        1 aatgattcct cttaccgatg tgttgagtat aaatgacgaa gaagcacgtt tattatcaaa
       61 agaatattcg ttgcgtaaag cagcaaaaat tattatggct atgggcccca aaaccctaat
      121 cataaaaaaa ggcgaacacg gagcactatt atttaatgga aacaatgttt tcttcgctcc
      181 cgcacttccg ttagaagagg tgtttgaccc tacaggtgca ggcgatacat ttgccggtgg
      241 tttcatcggt taccttgcaa aaacaagcga catttcattc gacaacatga aacgcgcaat
      301 tattttcggt tctgctatgg cttctttttg tgttgaaaaa tttggtcccg aaagattgtt
      361 atcgcttacc gataaagaaa taactgcacg tgtaaacgac ttttacgatt tggtagagtt
      421 tgatttgatt gatgcactca atatttaagc gctgattttt tttgctaata aattaaaaca
      481 ttagcaaagt caatagagtc gaacgaattg acagtataaa tatcagcatt gaaatttaca
      541 gaaggcgaat cgatggcaat tccatttgca gcactataag aaaaaataca atttcgtatt
      601 tcgcagtttg tatttgtatt accaccaata ataatcattg cttcgggatt cgatgagctg
      661 taattatttg caaagccaca atatttaata tcgcaatttt cgaaatgaca tgtggcacct
      721 atgtcgagcc caatcccttg ccaatagccc tttttgccta gctgaccaac aaactcaata
      781 cgattgttag ttgttccaat tgccgtaaaa cttgcaccat tttgcaagct atcatttgca
      841 taaaaaagtg aaagcttttg cattataaat tttgtgccgg gttgtattat taccgttgtg
      901 ccaatattaa atgtggagtc tactgtataa ggcacatcta actttggaat gttcagggtg
      961 cggtaagttt gcaggttacg tattgtgcga atataaatcc ggtcgaaggt gttaccggtg
     1021 tataaattat cattggtaag tatatcgcaa tttacatggt ctatcatcac cggttcggca
     1081 tagttgccgg taaatgtgtt gttgtgtatg ccaatattta cagccgcact atcgaggtat
     1141 aatccattag caaaattatc tttaatggtg caattttcca tcgacaccga tgcccctaac
     1201 acatttatgc atgagcgttt ttcaatttta taaatatcgg aacctgcatg ttgtatgatg
     1261 caatgtttca ttatttgtgt tacacctttc gagtataaat aaatgccacg ccacgagcca
     1321 cgcgtttttt ccaatcccag aatatttatt ttctcgtttg catttccttg ccaatcaatt
     1381 gatgcagcat ctgaaatttc aatgcccgaa cccggggtca tttctattgt tactcccggt
     1441 tgtattatca atcgtgcacc attagttact ttcatgcaaa tagcaatgcg ataatcaaca
     1501 ccttgcttat gatttattaa aatggtatcg tgggtaatat catcactcaa gactattgca
     1561 ccatcattgg ggaaatcatt ggaagtaaca gttttggtt
//
LOCUS       DAORQA010000373         1595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_15838_length_1595_cov_5.101299, whole genome shotgun sequence.
ACCESSION   DAORQA010000373 DAORQA010000000
VERSION     DAORQA010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15838_length_1595_cov_5.101299"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1595
                     /locus_tag="PK736_10010"
     CDS             <1..>1595
                     /locus_tag="PK736_10010"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRC33763.1"
                     /translation="YNSSGVCQWVKTGGSYQDEYGNAIAVDKAGNAYVVGLFMNNAVF
                     SGVTISAPAGRPDGFIATYGTAGTLTSLKKITSTDYESCKGITLDGQGHFYITGITDG
                     NTLFDSIQIQNNGNYDMFLAKYTINGTVRWVRTTQGDQWEEGRQVCCDAEGNVYIAGD
                     FYAFGTTPFPPYPFPGVGSFDILIAKYNRHGEFQWAQRGGSVQDDYAFAIATTDSGKV
                     YFTGSYEDNLTLGNTYLPNYTGPGGWTDMFYAKVRTDITTGNPPSSTYCAGTSFSLTF
                     VAHQAYKLTNVFTAQISDANGRFDNAINIGTLNANISSSIFCTIPTTVPFGIGYRVRV
                     IASDTLSFGNDNGTDITINTAPVVSLTANDFDICTGDSATLTTPHYNGNNYKWYYNNA
                     FVNGAVTNVYFAKQTGNYKVRVTNSFGCSVASPNKLITVNNYPTASITASGPLTYCST
                     DSVILTASSGVGYAYQWRKGTATVANATKITFKPATSGTYRVLVTNAAGCSTLSSSKK
                     ITILKPVAEVSIASITFSLMLVS"
BASE COUNT          474 a          325 c          335 g          461 t
ORIGIN      
        1 gtataatagt agtggcgtgt gtcaatgggt aaaaaccgga ggtagttacc aggatgaata
       61 tggcaatgca attgcagttg acaaagccgg caatgcatat gttgtaggtt tgtttatgaa
      121 taatgcagta ttcagcgggg ttactatttc agctcctgcc ggtcgtcccg atgggtttat
      181 agcaacatat ggcacagccg gtacacttac atcgctcaaa aaaattacca gtaccgatta
      241 cgagtcttgt aaaggaataa cacttgatgg gcaaggacat ttttacataa ctggtatcac
      301 ggatggcaat acactatttg attccataca aattcaaaac aatggcaatt acgatatgtt
      361 cttagccaaa tatacaatta atggcacagt gcgttgggtg cgcaccacac aaggcgatca
      421 atgggaagag ggcagacagg tgtgttgcga tgcagaaggc aatgtgtata tagcaggaga
      481 tttttacgcg tttggaacta cgccattccc accctaccca tttcctggtg taggtagctt
      541 tgatattttg attgcaaagt ataacaggca tggtgagttt caatgggctc aacggggagg
      601 cagtgtgcaa gacgattatg catttgccat agcaactact gactcgggaa aagtttattt
      661 taccggcagc tacgaagata accttacact tggcaatact tatttaccaa attataccgg
      721 accgggtggc tggacggata tgttttatgc taaagttcga acagatatta caacaggtaa
      781 tccaccctca agcacatatt gcgctggtac aagtttttca ttaacgttcg tagctcacca
      841 ggcttataag cttaccaatg tatttacagc acagattagc gatgccaacg gaagatttga
      901 caatgcaatc aatattggaa ctttaaatgc aaatatatca agcagtatct tctgcacaat
      961 tccaaccaca gtgccgtttg gcattggtta ccgtgtgcgc gtaatagcca gtgatacgct
     1021 ctcattcggt aatgataatg gcactgatat tactattaat actgctcctg tagtttcatt
     1081 aactgcaaac gactttgaca tttgcacagg agatagtgca acacttacca caccgcatta
     1141 taatggtaat aattataaat ggtattataa caatgccttt gtaaatggag cagttactaa
     1201 tgtgtacttt gctaagcaaa ccggaaacta taaagttcgt gttaccaatt cttttggttg
     1261 ttctgttgca tcgcccaaca aattaattac agttaataat tatcccacag cttcaattac
     1321 cgcttcggga cctcttactt attgttccac cgatagtgta atactcactg catcgagcgg
     1381 tgtgggttat gcttatcaat ggcgaaaggg aacagccact gtagctaatg ccaccaaaat
     1441 cacctttaag ccggctactt ctggaacgta tagggtgtta gttacaaatg ctgccggctg
     1501 cagtaccctc agttcttcaa aaaaaataac catactgaaa cctgttgctg aagtcagtat
     1561 tgcctccatt accttttcgc taatgcttgt ttcta
//
LOCUS       DAORQA010000374         1580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_16009_length_1580_cov_32.041967, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000374 DAORQA010000000
VERSION     DAORQA010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16009_length_1580_cov_32.041967"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..1382
                     /gene="phaC"
                     /locus_tag="PK736_10015"
     CDS             324..1382
                     /gene="phaC"
                     /locus_tag="PK736_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HRC33764.1"
                     /translation="MTVNANEVMEDVSRVNQKLMKGYQTLQTIDEVDVAITPKTLVWQ
                     SDKVKLYHYDRATPAKCKVPVLVSFAIMNRHDVLDLQQDRSLMKKLLDEGLDIYIMDW
                     GYPGKMDRYLTMEDYILGYMNDAVDFIREAHGVEKIHKMGICQSGTFSTIYAALYPEK
                     LLSLTVYVAPFDFSDNNNMLYKWTKYIDIDTMVDTLGVIPADMLNMAFGMLKPSMDIS
                     KYLGMMDSLEDREKILNFLRMEKWKNDCPDLAGEMFRKYIKDLFRDNLLVQGKMELGG
                     QKIDLKNVTMPFLNVFATEDNIIPNDSTTAVQKHIGSKDKELYAFPGGHIGVFVGAKS
                     QRELAPKVAQWVMSKTKK"
BASE COUNT          490 a          282 c          334 g          474 t
ORIGIN      
        1 ccccgaccgc agtgtcgggg aatgcacgaa tgaatgccga ataatacttt gccactttgc
       61 cgcgcctgcc ccgatcgcag tgtcggggaa tgcaagaatg aatgcctaat aatattttgc
      121 cacgaaaaac actgaatgca ctaaagaatt ctgaaaaaat tcaaatgctt tttgcataaa
      181 tgcattcaat gaaacacaga taattgcgac tgaattgtat ttgtttttca ccataaactt
      241 tccattcacc ctcaattttg aatggtattt tttgctgctc aaaacatatt tctaccttcg
      301 cggcaaatta aaataaaaac acaatgacag taaatgcaaa tgaagtgatg gaagacgtaa
      361 gccgcgtgaa tcaaaaattg atgaagggct accaaacatt gcaaactata gatgaagtag
      421 atgtagccat tacccctaaa acactggtgt ggcagagcga taaagttaag ttgtaccatt
      481 atgaccgcgc tacgcctgcc aaatgtaagg tgccggtttt ggtatcgttt gccataatga
      541 acaggcacga tgtgcttgat ttacagcagg accgcagcct gatgaaaaaa ttgctggacg
      601 aaggtctcga tatctatatc atggattggg gctatccggg caaaatggac cgttacctta
      661 ctatggaaga ttatatactg gggtatatga acgatgctgt tgattttatt cgtgaagcac
      721 atggagtaga aaaaatacat aaaatgggca tttgccagtc gggcacattc tccacaattt
      781 atgctgcact gtatcccgaa aaattattat ccctaactgt atacgtagct ccgttcgatt
      841 tttcggacaa caacaatatg ttgtacaaat ggacaaagta catcgatata gatacaatgg
      901 ttgacaccct tggagtaata ccagccgata tgctcaatat ggcttttgga atgctgaagc
      961 catcaatgga tataagtaaa tatttgggta tgatggattc gctggaagac cgtgagaaga
     1021 ttttgaactt tttgcgtatg gaaaaatgga aaaacgattg tcctgatttg gccggagaaa
     1081 tgtttcgtaa gtatataaaa gatttgtttc gcgataacct gcttgttcag ggtaaaatgg
     1141 aactgggcgg acaaaaaata gatttgaaaa atgtcacgat gccgttttta aatgtattcg
     1201 ccaccgaaga taatattatt cccaacgact ctaccactgc agttcaaaag cacattggaa
     1261 gtaaggataa ggagttgtat gcatttcccg gtggacatat aggtgtgttt gttggcgcta
     1321 agagtcagcg cgaattagca cctaaagttg cacagtgggt gatgtcgaaa actaaaaagt
     1381 aattaccctt cacatatttt attagccatt gtaatgttta tcattgcaat ggctttgtta
     1441 tttttaccaa tctttttttc cttgtatatg actttaatat tgtaccgttt tctgtggata
     1501 ctgttttgcc ttattattat aggcaatagt ggtttctttt attcgcggag ggtacatgta
     1561 ctaaattttc agttggcatt
//
LOCUS       DAORQA010000375         1573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_16099_length_1573_cov_15.716733, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000375 DAORQA010000000
VERSION     DAORQA010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16099_length_1573_cov_15.716733"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PK736_10020"
     CDS             complement(<1..995)
                     /locus_tag="PK736_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33765.1"
                     /translation="MKRILFLCLLLIAGSQISHAQNCNSICNGDFEDSIVVPGPNLIL
                     IDSTEMPCWKTTASNGIFEVWGTGFQNVPSYSGQQFVELNAQFVSTLYQNITANPGSL
                     INIAFAHRGRWGLDSLSVSVGPVGGPYVTLGTFGDDSSAWGYYTLNYALPNVGTNFSL
                     RFNSVYAAGGNPTVGNFLDDVSVTTSCIVIEDTCFSDVTKFTMADSLGFVAFQWSFDD
                     PSSGPLNNAFDASVSHLFTAPGLYNVQLIRVFNFAPYSDTATYKVNINPSPVVNIGND
                     TTICSGQTIIVQCTNVGTYIWQNGSTNFYINVNATGTYTVTVTTLAGCAGTDSISV"
     gene            complement(1178..>1573)
                     /locus_tag="PK736_10025"
     CDS             complement(1178..>1573)
                     /locus_tag="PK736_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33766.1"
                     /translation="NVNNPTTILGTNYFFVPSIAGQYYVVVVDSIGCDVSSSIYNSNV
                     GMNENLDGNIQVLVNQNENTIFINCENSMSQIFVVKLYDGIGREVSKIENTSNTKSLK
                     LQMPTQAGTYILSIETNNKIYRSKIVVGK"
BASE COUNT          495 a          309 c          285 g          484 t
ORIGIN      
        1 acacttatgc tatcggtgcc ggcacaacct gcaagtgtag taacagtaac agtatacgta
       61 ccggtagcat ttacattaat ataaaaattt gttgacccgt tttgccatat atacgttccc
      121 acatttgtac attgaacgat gattgtttgt ccgctgcaaa tagtagtatc gttaccaata
      181 tttactacag gcgatgggtt gatgtttact ttgtatgtgg ctgtgtcgct ataaggcgca
      241 aaattaaaaa cacgaataag ctgcacgtta tataagccgg gcgcagtaaa taaatgacta
      301 acagaggcat cgaaggcatt gttaagtggc cctgatgatg ggtcatcgaa actccattga
      361 aaagccacaa accccaatga atcggccatg gtaaattttg taacatcgct aaagcaagta
      421 tcttctataa ctatacatga agtagtaaca cttacatcat ctaaaaaatt gccaacagta
      481 ggattaccgc ctgcagcata taccgagttg aaccgcaagc taaaattggt accaacattc
      541 ggcaaagcat aattaagtgt gtagtagccc catgcagaac tgtcatcgcc aaaagttccc
      601 aaagttacat atggcccacc aacaggacca accgaaacac tcaatgaatc gagaccccac
      661 cgaccgcgat gtgcaaaggc gatattaatt aacgacccgg gatttgctgt aatgttttga
      721 tataaagtgg aaacaaactg cgcgttgagt tctacaaatt gttggccact gtatgaaggc
      781 acattctgaa aaccggtacc ccaaacttcg aatattccat ttgaggccgt agttttccaa
      841 catggcattt ctgtactatc aatcaatatt aaattggggc cgggcaccac gattgaatct
      901 tcgaaatcgc cattgcaaat actgttgcaa ttttgtgcat gcgatatctg cgagcctgca
      961 atcaacaaca agcacaggaa tagaattctt ttcatttttg agttttgttt atttttcatt
     1021 tacgaaacaa tagccagtgt aatgctgaga tattggttcg tgcattttgc gccaaagcta
     1081 attaattttt ttatggaaga attaattgaa tttagtctca ttcgaaaagc ccattttcta
     1141 ttaagttttg tcttgataat tttgcagtaa gaactattta tttcccaacc acaatcttac
     1201 tcctgtatat tttattattt gtttcaatcg ataaaatgta agtgccggcc tgtgttggca
     1261 tttgcagttt tagacttttc gtgtttgaag tattttcgat tttgctcacc tcacgtccta
     1321 taccatcata gagtttaaca acaaaaattt gcgacattga attttcgcaa tttataaata
     1381 ttgtattttc attttggtta accagtactt gaatatttcc gtctaaattt tcgttcatac
     1441 ccacattaga attatagata gaggacgaaa catcacaacc aatagaatcg accaccacta
     1501 cataatattg tccggcaata gatggtacga aaaaataatt ggtgccaaga atggttgtag
     1561 gattatttac att
//
LOCUS       DAORQA010000376         1570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_16151_length_1570_cov_4.807921, whole genome shotgun sequence.
ACCESSION   DAORQA010000376 DAORQA010000000
VERSION     DAORQA010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16151_length_1570_cov_4.807921"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1102
                     /locus_tag="PK736_10030"
     CDS             83..1102
                     /locus_tag="PK736_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33767.1"
                     /translation="MRLPLFIIKYIYYEYWPWWLFYVPMLPLWFYLSLRNRSFAYFSV
                     TNPGIYLGGFFGESKKDIMALIDNAYMAQSVYIENEIDFESVKSKIKNMPYPLIAKPD
                     VGERGNDVEKIIDAVQLQQYHSKINAPYIIQEFITYDIELGILYSRMPNEEKGIVSSV
                     TFKKFMSVMGDGVNTVLQLMQKNTQSRFQIKRMLSEQSTLMQSIPAKGETILLEPIGN
                     HCRGTAFVNFNHIINDDLHRVFDNICKPIQGFYYGRFDLKVKSIEDLYKGENIKIMEL
                     NGASSEPGHIYDKHYTLLKAYKDLSQHWIRLSNISRYNMRNGYKPVSFSTIVKTYFKF
                     IVFKK"
     gene            complement(1365..>1570)
                     /locus_tag="PK736_10035"
     CDS             complement(1365..>1570)
                     /locus_tag="PK736_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33768.1"
                     /translation="GIGLMKYFSQHLFLTADATFRYFPARWYSKKFDNNIDPDTGLRT
                     AVSYHVVDKPWSVGLQIALNYHF"
BASE COUNT          502 a          304 c          296 g          468 t
ORIGIN      
        1 cataaaacta atgccggcag cgaaataatt tacgaagatg tgattcaaaa aaaattgcat
       61 cgcaaccttg tgatataaaa ctatgcgcct tccacttttt attattaaat atatctatta
      121 cgaatattgg ccgtggtggc tgttttatgt tcctatgttg ccgctgtggt tttacctttc
      181 gttgcgcaac cgctcatttg cttattttag tgttaccaat ccgggcatat accttggtgg
      241 tttttttggt gagagtaaaa aagatattat ggcccttatc gataatgctt acatggcaca
      301 atcggtgtac atcgaaaacg aaatagactt tgaaagtgta aaaagcaaaa ttaaaaacat
      361 gccatatccg ctaattgcta aacccgatgt aggagaacgt ggcaatgatg tagaaaaaat
      421 aattgatgct gttcaacttc aacagtatca ttcaaaaata aatgctcctt acattataca
      481 ggaatttata acttatgata ttgagttggg aatactttat tcacgtatgc ccaatgagga
      541 aaaaggcata gtgagttcgg ttactttcaa aaagtttatg tctgtgatgg gcgatggagt
      601 aaatactgtt ttacaattga tgcagaaaaa tacacagtca cgatttcaaa taaaacgaat
      661 gctatccgag caaagtactt taatgcaatc cattcctgcc aaaggtgaaa cgattttgtt
      721 agagcccatt ggcaatcatt gccgcggaac agcgtttgta aattttaatc atataattaa
      781 tgatgatttg catcgtgttt tcgataacat ttgcaaaccg atacaagggt tttattacgg
      841 ccgcttcgac ctcaaagtaa aaagcattga ggatttatat aaaggcgaaa acataaaaat
      901 tatggagctt aatggcgcca gttccgagcc gggccatatt tacgataagc attacacatt
      961 gctaaaagcc tataaagatt tatcgcaaca ttggatacgt cttagcaata tttcgcgcta
     1021 taatatgcgg aatggatata agcctgtttc attttcaaca atagtaaaaa cctatttcaa
     1081 gtttattgtt tttaaaaaat aggttaccca aacagttaga atctattcgg ctttcattcg
     1141 tgcattcccc aacactgtgg tcggggcagg cgtggcaaac gaaatgagcc gcaaagcatt
     1201 tctctttccg tcattgcgat cctctttcct ctcgtcattg cgagcgcagc gaagcaatct
     1261 cagcgtcaac aagtccactt cacgaggagg ttgcttcagt cgtacctcct tcgcaatgac
     1321 ggaaggcaat taaccattcg tggcaaaaaa gcatttgcat tttatcaaaa atgataattc
     1381 aatgcaattt gcaaacccac cgaccagggc ttatctacca catgatagct caccgcagtg
     1441 cgcaacccag tatcggggtc aatattatta tcgaattttt ttgaatacca ccgcgcagga
     1501 aaatatctaa aagtagcatc ggctgttaga aataaatgtt ggctgaaata tttcatcaac
     1561 cctattccgc
//
LOCUS       DAORQA010000377         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_16310_length_1557_cov_8.063249, whole genome shotgun sequence.
ACCESSION   DAORQA010000377 DAORQA010000000
VERSION     DAORQA010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16310_length_1557_cov_8.063249"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>1557
                     /locus_tag="PK736_10040"
     CDS             21..>1557
                     /locus_tag="PK736_10040"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRC33769.1"
                     /translation="MDRTGHQLGYGQYGLNSNDITNIYSGFGGGDVIRVFNNACTYEV
                     ESNGVAGPITTSGNQNYQGLGGGEFYWEDNYIFWHDETSFGSLGMQPGDSVVTVAVAN
                     PQTFFGAGGISHFSNIDGSKSYGFQVYDPSIAGTCANANGLGDQEYGFDAEDIEIGNI
                     VWDDTHNFGIQDADEKGISGVNIQLIRVSDNAVIGNATSNSSGQWYFNKSNVNLNGAS
                     GLLPNTSYKLRVAAAQFNNTGSGPLANKDITNSGMVGNGEPGMSDNDATLVSGKAEIA
                     FTTTKNGASNHNLDFGFKSSLGCPLVATCSSTNTTCNGGADGEVSVTTVGAVGAVDYL
                     WSTASTNAAVNGLAAGTYTVTVTDSQGCSDMCSVTIGQPTAVSASCSGTNVTINGGNN
                     GSVSVTANGGTPGYTYLWNNGNTNATQSGLAASTYTVTVVDANACTGTCSITISQPLP
                     LSGSVVITDINCNGGNDGEIALTILGGVTPYSYLWSNGATTQDINNLFAGNYDVTVTD
                     NAGA"
BASE COUNT          474 a          259 c          366 g          458 t
ORIGIN      
        1 gttcaatgat attaggtttt atggatcgta ccggtcatca gttgggttac ggtcaatatg
       61 ggcttaactc aaatgacatt acaaatattt acagtggttt tggtggtggt gacgtaataa
      121 gggttttcaa taacgcttgt acgtatgaag tggaaagtaa tggagtagcc ggaccaatta
      181 ctactagcgg aaatcaaaat tatcaaggct taggtggtgg tgaattttat tgggaagata
      241 attatatttt ctggcatgat gaaacctctt ttggctcatt gggaatgcaa ccgggagatt
      301 cggtagtgac tgttgcagtt gcaaatccac aaacgttttt tggcgctggt ggtatcagcc
      361 atttttcaaa tattgatggt tctaaatcat atggttttca agtttatgac ccttcaattg
      421 caggtacttg tgccaatgca aacggattgg gcgatcagga atatggcttt gatgccgaag
      481 atattgaaat tggaaatatt gtttgggacg atacgcacaa ctttggtata caggatgctg
      541 acgaaaaagg aatatcaggg gttaatatac aacttatacg tgtttctgat aatgccgtta
      601 ttggtaatgc aacttcaaac tcaagtgggc aatggtattt taataaatcg aatgtcaatt
      661 taaatggagc ctcaggatta ttaccaaata caagttataa attacgtgta gctgctgcac
      721 aatttaataa cacaggtagt ggacctttag caaataaaga tattacaaac tcaggtatgg
      781 ttggaaatgg cgagcccggt atgagcgaca atgacgcaac actggtgagt ggaaaagcag
      841 aaatagcttt caccacaact aaaaacggtg ccagcaatca caatctcgat tttggtttta
      901 agtcatcttt aggatgtcct ttagttgcca catgctcttc aaccaataca acatgtaatg
      961 gtggtgccga tggcgaagta agtgtaacaa cagtgggtgc tgtcggtgct gtagattatt
     1021 tatggagcac cgcctctaca aatgcagcag ttaacggttt agcagcaggt acgtataccg
     1081 taacagttac cgatagccaa ggatgttcgg atatgtgcag tgtaactatt ggccaaccta
     1141 cagcggttag cgcaagttgc tcaggcacaa atgttacaat aaatggagga aacaatggta
     1201 gtgtgagtgt tacagcaaat ggtggtactc ccggatatac atatttatgg aacaacggta
     1261 acacaaatgc tacgcaatcc ggattggcag cttctacgta tactgttact gttgtagatg
     1321 caaacgcttg cacaggaaca tgttctatta caattagtca acctttaccg ctaagtggat
     1381 cggttgtgat tactgacatt aattgtaatg gtggtaacga tggcgaaatt gctttaacca
     1441 tattaggtgg tgtaactcca tattcatact tatggagtaa tggggctacg acacaggata
     1501 tcaacaattt atttgccggc aattatgatg taactgtaac tgataatgca ggtgcaa
//
LOCUS       DAORQA010000378         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_16314_length_1557_cov_4.625166, whole genome shotgun sequence.
ACCESSION   DAORQA010000378 DAORQA010000000
VERSION     DAORQA010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16314_length_1557_cov_4.625166"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>876
                     /locus_tag="PK736_10045"
     CDS             <1..>876
                     /locus_tag="PK736_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRC33770.1"
                     /translation="SIYNSNGKLLVADDALLGTQVWNDEITTYKKMHSDEKYIGLGEK
                     TGPLNKRGYSFTNWNTDDFAYTTEADPLYKTIPFYIGIHDSVCYGLFLDNSYKSNFNF
                     GGSTDERMMSFTIESGEMNYYILCGDNIERVIKNYTQLTGTMPMPPLWSLGYQQCRWS
                     YYPDKEVLTVARTFRDKKIPCDVIYNDIDYMDRYRIFTFNKEKFPEPKRYLDSLAAMG
                     FKLAVIVDPGIAIADDYAVHKDGLTKSVYVKYPTGDLYIGNVWPGRCNFPDFTLPMAR
                     QWWGANFKTLTQAGVR"
     assembly_gap    877..898
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <899..>1557
                     /locus_tag="PK736_10050"
     CDS             <899..>1557
                     /locus_tag="PK736_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRC33771.1"
                     /translation="NEPAAWGQNIPNQIIFDYDGNKTTMKQARNVYGMQMSRATFEGI
                     RKILPNERVFNLTRATYAGGQRYSAVWTGDNDASDDHMMMSSRMIQSLNLSGFSFAGA
                     DIGGFANEPSKELFIRWLSMAAYTPFFRNHTMINTRDKEPWALGEETESMARECINTR
                     YKLLPYIYTAFYESHAYGKPLVRSLCMENMNDDNVYNENYQNQFMLGNALLICPVSSK
                     D"
BASE COUNT          482 a          298 c          333 g          422 t
ORIGIN      
        1 tccatataca attcaaatgg aaaattattg gtggccgatg atgctttgct gggcacacaa
       61 gtgtggaacg atgaaataac tacctacaaa aaaatgcaca gcgatgaaaa atatatcgga
      121 ctgggcgaaa agacagggcc actaaacaaa cgtggatatt cctttacaaa ctggaatacc
      181 gatgattttg catacaccac cgaagccgat ccactttaca aaaccatacc attttatatt
      241 ggcattcacg atagtgtttg ttatggtttg tttctcgaca attcatataa atcgaatttt
      301 aatttcggtg gctccacaga cgaacgcatg atgagtttta cgatcgaaag tggcgaaatg
      361 aattactata tattgtgtgg cgataatatt gaaagagtaa taaaaaatta cacgcagctt
      421 acgggcacca tgcctatgcc gcctctatgg tcgttgggtt atcagcaatg ccggtggagt
      481 tattaccccg ataaagaagt gttgacagtg gcccgtactt tccgcgataa aaaaataccc
      541 tgcgatgtaa tctacaacga tattgattat atggacaggt acagaatttt tactttcaat
      601 aaagagaaat ttcctgaacc taaaagatac ttggattctc tcgctgctat gggctttaaa
      661 cttgccgtta tagttgaccc cggcattgcc attgccgatg attatgctgt gcataaagat
      721 ggactaacta agagtgtata cgttaagtat cctaccggtg atttatatat aggaaatgta
      781 tggccgggcc ggtgcaactt tcccgatttt actttgccta tggcacgaca atggtggggg
      841 gcaaatttca aaacccttac tcaggctggt gtacgtnnnn nnnnnnnnnn nnnnnnnnga
      901 atgagcctgc agcatgggga caaaatattc ccaaccaaat tatttttgat tacgatggaa
      961 ataaaaccac aatgaaacag gcacgaaatg tttatggtat gcaaatgtcg cgcgctacat
     1021 tcgaaggcat acgtaaaatt ttgcccaacg aaagagtatt caatcttacg cgtgctactt
     1081 atgccggtgg tcaacgttac agtgctgttt ggacaggcga taacgatgcc agcgatgacc
     1141 acatgatgat gagtagccgc atgattcaaa gtctaaatct tagtggattt tcgtttgccg
     1201 gtgctgatat aggaggtttt gcaaacgaac caagcaaaga attattcata cggtggttgt
     1261 cgatggcggc atacacgcca ttttttagaa accacaccat gataaatacg cgcgataagg
     1321 aaccatgggc gcttggcgaa gaaaccgaaa gcatggcacg agaatgcatc aatacacgtt
     1381 ataaattatt accctatatt tatactgcat tttacgaatc gcatgcttac ggtaaaccgt
     1441 tagttcgcag tttatgtatg gaaaatatga acgatgacaa cgtgtacaat gagaattatc
     1501 aaaaccaatt catgttgggg aatgctttgc tcatttgccc cgtaagcagt aaagatc
//
LOCUS       DAORQA010000379         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_16323_length_1556_cov_6.590273, whole genome shotgun sequence.
ACCESSION   DAORQA010000379 DAORQA010000000
VERSION     DAORQA010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16323_length_1556_cov_6.590273"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="PK736_10055"
     CDS             <1..1468
                     /locus_tag="PK736_10055"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33772.1"
                     /translation="FNSIATAQQGVNNLWYLGYSNVVGPPNYGGITIDFFTSTPTIFA
                     QQRKMDLLHTHSNISDSLGNILFYTNGTFICDATDSIMQNGDSISPTNLIPVYPNALW
                     IPQACLTIPYPGYANKYLLFHSTIDKAWTTCLHLYYSEIDMTLNNGLGAVTTDKNVAL
                     INDTLNLGKITACKHGNGRDWWVVCFQGNSDTIYKFLVTPFGIDGPFKQAIGTKREVS
                     YGQIKFSSDGNKFAYFFAQGPPNSTNISQFEILDFDRCSGIFSNAKNIFIGASYGLGG
                     GVEFSPNSQYVYVSNIDSVYQYDVTASNIDSTKIVVAVWDSFYDGGPPFAVLFDPIEL
                     APDGKIYICSGNGTHYLSVINNPDSQGLACNLTQHSVVLPAYYANTLPNHPNYFLGAN
                     GLCNNLSYESLESWKVKKVTVFGNPTHDKFTLWFPVDKDVGWLEIYDVNGSCIRRERV
                     SQWSQYKTVDISLFSEGVYFCRMVWPDGEGSVKVVRLE"
BASE COUNT          474 a          250 c          332 g          500 t
ORIGIN      
        1 gttcaatagt atagccacag cacaacaagg tgtaaataat ttatggtatt tggggtattc
       61 aaatgtagtt ggccctccaa attatggagg cataactatt gattttttta caagcacacc
      121 aaccatattt gctcaacagc gcaaaatgga tttactacat acacattcaa atataagcga
      181 tagtttgggt aatatattat tctatactaa tggtactttt atatgcgatg ccacagatag
      241 tataatgcaa aatggcgatt ctattagccc tacaaattta attccggttt acccaaatgc
      301 actctggatt ccccaggcat gtttgacaat tccatatccc gggtatgcta acaaatatct
      361 actttttcat tctacaattg ataaagcatg gactacgtgc ttgcatttgt attacagcga
      421 aatagatatg acattgaata atggattggg cgcagtaact acagataaaa atgttgcctt
      481 aataaatgac accttaaact taggtaaaat aacagcctgc aaacatggca atggtagaga
      541 ttggtgggtg gtatgctttc agggaaatag tgatacaatt tacaaatttc tggtaacacc
      601 ttttggtatt gatgggccgt ttaagcaagc aataggtact aaacgagaag ttagctatgg
      661 gcaaataaag ttttcaagtg atggtaataa gtttgcgtac ttttttgctc aaggtcctcc
      721 aaactcaaca aacatttcac aattcgagat tttggatttc gatcgttgta gtggtatttt
      781 tagtaatgca aaaaatattt ttatcggtgc aagttatggt ttaggtggcg gtgtagaatt
      841 ctcccccaac tcacaatatg tgtatgtatc aaatatagat tcagtttatc aatatgatgt
      901 taccgcaagc aatatagatt caacaaaaat tgtagtagct gtttgggata gtttttatga
      961 tggcggccct ccctttgctg tattatttga ccctattgaa cttgcgcctg atggtaaaat
     1021 ttatatttgc tcaggaaatg gtactcatta tttgagtgtt ataaataacc ctgatagcca
     1081 gggtttagca tgtaacttaa cgcagcactc agttgtatta cctgcatact atgctaatac
     1141 cctccccaac caccccaatt actttcttgg tgcaaatgga ttgtgcaata atttgagtta
     1201 tgaaagttta gaaagttgga aggttaagaa agttacggtg tttggtaacc ctacgcatga
     1261 taaatttaca ttgtggtttc cggtagataa ggatgtgggt tggttggaga tatatgatgt
     1321 taacggtagt tgtatacgta gggagagagt aagccaatgg agccaatata agactgtgga
     1381 tattagtttg ttttcagagg gggtttattt ttgtaggatg gtttggcctg atggtgaggg
     1441 gagtgtgaaa gtggttaggt tggagtagtt cgtatgataa gcaaatagaa gatacggaaa
     1501 ttgccctcgc tcacccctga ccctaaaggg cgcaaccgca cttatcttgt ttacag
//
LOCUS       DAORQA010000380         1544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_16457_length_1544_cov_8.046340, whole genome shotgun sequence.
ACCESSION   DAORQA010000380 DAORQA010000000
VERSION     DAORQA010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16457_length_1544_cov_8.046340"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..>850
                     /locus_tag="PK736_10060"
     CDS             382..>850
                     /locus_tag="PK736_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRC33773.1"
                     /translation="MGNKEESEKLLAAKQMSEAQDKKEIIKQYRSLLRSCKAYVTSQD
                     YKLIRKAFDLSLNAHKDMRRRSGEPYILHPLAVAQIVAQEIGLGPTSIVCALLHDTVE
                     DTDITLVDIEKMFGARVSKIIDGLTKISGVFDHTSSIQAENFRKMLLTLSDDVR"
     assembly_gap    851..860
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <861..>1544
                     /locus_tag="PK736_10065"
     CDS             <861..>1544
                     /locus_tag="PK736_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HRC33774.1"
                     /translation="RLHNMRTLGSMPRDKQLKIYSETAYLYAPLAHRLGLYSIKTEMD
                     DLVLKYTEPQTYKDIVKKLQNTKKERDKFIADFTKPIEEALRKEGFQNFEIKGRPKSI
                     NSIWRKMKKQDIPFEEVYDLFAIRIILDSQPDKEKSDCWAAYSIVTDFYRPNTDRLRD
                     WISNSKANGYESLHTTVMSNTGRWVEVQIRTQRMDEIAEKGYAAHWKYKESSNESAVD
                     SWINRIREVL"
BASE COUNT          512 a          298 c          313 g          411 t
ORIGIN      
        1 tgcctcttaa aaaaaaataa cgagctaagt attccttaag ttgacgtgta tgcccgtagg
       61 gactacatct tggtaacata aggaacgccc tcttggtttt tgtcctgtaa ggactaccca
      121 atgactgtat aagcagtctg cgaaatgggt agttactacg taacaaaata catgctgttt
      181 ttgattatta ccaagcggta atccctacgg gataaaacta attctggtaa acttaatgac
      241 cttaggcttt aaccctgagt agttacgttt tgctaaatgt gaaacattct ttgaataaaa
      301 agaaactaaa taaaaacaat ttcacttcac aaataaaatg ttgtgggcat ttttaattta
      361 tatttgtagc aaataactaa gatgggaaat aaagaggaaa gtgaaaaatt attggctgcc
      421 aaacaaatga gcgaagcgca ggataaaaaa gaaattatca agcaatatcg ttcgttgcta
      481 aggtcgtgca aggcctatgt tacatcacag gattataaac ttattcgcaa ggctttcgat
      541 ttatcgctca atgcacacaa agacatgcgc aggcgttctg gcgaacctta tattttacat
      601 ccacttgccg tagcacaaat tgtggcacag gaaattggcc ttggtcccac ttctattgtg
      661 tgtgcattgt tgcacgatac agtagaagat accgacatta ctttggtcga catcgaaaaa
      721 atgtttggtg cccgtgtatc gaaaattatt gatggcctta caaaaataag tggtgtattc
      781 gatcacactt catctataca agccgaaaat ttcagaaaaa tgttgctcac cctaagtgac
      841 gatgtgcgtg nnnnnnnnnn ccgtttgcac aacatgcgca cattgggaag catgccacgc
      901 gataaacaac ttaaaattta cagcgaaaca gcttacctgt atgcaccgct tgcacaccgc
      961 cttggactat attctataaa aactgagatg gacgatttgg ttttgaagta taccgagcca
     1021 caaacgtata aggatatagt taagaaattg caaaatacaa aaaaagagcg cgataaattt
     1081 attgctgatt ttacaaaacc cattgaggaa gcattgcgga aagaaggctt tcaaaatttc
     1141 gaaattaaag gccgcccaaa atccatcaac tccatttggc gcaaaatgaa aaagcaggat
     1201 attcctttcg aagaagtgta cgatcttttt gctatacgta ttatactcga ttcgcaacct
     1261 gataaagaga aaagtgattg ctgggcagcc tactccattg ttaccgattt ttaccggccc
     1321 aataccgatc gtttgcgcga ctggatatca aactctaaag ctaatggata cgagtcgttg
     1381 cacactaccg taatgagcaa tactggccgc tgggtggaag tgcaaatacg cacgcaacgc
     1441 atggatgaga ttgccgaaaa aggatatgcc gcccactgga aatacaaaga gtcgagcaac
     1501 gaaagtgctg ttgattcatg gataaaccgt atacgcgaag tgct
//
LOCUS       DAORQA010000381         1542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_16497_length_1542_cov_3.670477, whole genome shotgun sequence.
ACCESSION   DAORQA010000381 DAORQA010000000
VERSION     DAORQA010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16497_length_1542_cov_3.670477"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /gene="ftsA"
                     /locus_tag="PK736_10070"
     CDS             complement(<1..770)
                     /gene="ftsA"
                     /locus_tag="PK736_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRC33775.1"
                     /translation="MSKNLIIAGLDIGTTKICAIVGMKNENGKVEILGMGKAESEGVS
                     RGVVSNIEKTIASIKLAVAEASRVSNVVIDNVYVGIAGGHIKSLQHSGMLTRKDLETE
                     ISQADIDIIVNDMHKLAMSPGEEIIHVIPQDFTIDSERGIKDPRGHAGVRLEAKCHIV
                     TGQITAAKNIHKCVERSGLKCTDLILEPLASADAVLTEEEKEAGVVLIDIGGGTTDIA
                     IFHDNIIRHTAVIPFGGNSVTDDIRQGCALIRKDAEAL"
     gene            complement(807..>1542)
                     /locus_tag="PK736_10075"
     CDS             complement(807..>1542)
                     /locus_tag="PK736_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HRC33776.1"
                     /translation="KELQIKIVDSDQKGFIDNSDVINILRESDKFPTGKNIVDINTAL
                     LETILETNPYIENAEVFSTLDGVLHVEVSQREPIIRIFNANNECFYIDEKGHSMPVSD
                     KFSMNVITANGYINDNAASTKIKMINDTEADTLKQIKTVDQLFYIASSIKNDTLWNAQ
                     LVQMYVNSKNEIELVPRVGNHIILLGGVNNLREKFDKLLIFYRKGLNKKGWTDYAAIN
                     LSYKGQVVALKRTPIVTAKADTTISN"
BASE COUNT          401 a          343 c          268 g          530 t
ORIGIN      
        1 ttcaatgctt cggcatcttt gcgtatgagt gcacaaccct gacgtatatc atcggtaacg
       61 gagtttcctc caaaaggaat aactgctgta tgacgaataa tgttgtcgtg aaaaatagca
      121 atgtcggttg tgccaccgcc aatatcaatc aatactacac ctgcttcttt ttcttcttcg
      181 gtaagtacag catcggccga tgcaagtggt tcgagtataa ggtcggtaca ttttaatccg
      241 ctgcgctcta cacacttgtg aatgtttttt gccgcagtaa tttgccctgt aacaatatga
      301 cattttgctt cgaggcgaac acctgcatgt ccgcggggat cttttattcc gcgttcacta
      361 tcgatagtga aatcctgagg gataacatga ataatttctt cgcccggtga catggccagc
      421 ttatgcatat cgtttacaat aatatctata tcagcctggc ttatttctgt ttccaaatct
      481 ttgcgcgtaa gcatacccga gtgctgcaaa cttttaatgt ggccaccggc aatacctaca
      541 tacacattgt cgattacaac attgcttaca cgcgaagcct cagcaacagc caactttata
      601 gaggctatag ttttttcgat attggaaacc acaccgcggc taacgccttc cgattcggcc
      661 ttgcccattc caagtatctc aactttacca ttttcatttt tcatgcccac gatggcacat
      721 attttagtgg tgccaatatc gaggcctgcg attattaaat tcttgctcat tttcttttat
      781 atgctaatgt acgtatatag ttattgttaa ttcgaaattg ttgtgtcggc tttcgctgtt
      841 actatcggtg ttcgctttag tgctaccacc tgccccttat acgacaagtt gattgcagca
      901 tagtctgtcc aacctttttt attcaaccct ttgcgataaa aaattaaaag cttatcaaat
      961 ttttcgcgca aattatttac tccccccaat agtataatat gattgccaac acgcggcact
     1021 aattcaattt cgttcttact gtttacatac atctgcacta actgtgcatt ccaaagcgta
     1081 tcgtttttga tagagctggc aatgtaaaaa agttgatcaa ccgtcttaat ctgcttcaat
     1141 gtgtcagctt ctgtatcatt tatcattttg atttttgtgc ttgctgcgtt gtcatttata
     1201 tatccgtttg cagtaatcac attcatcgaa aacttgtccg aaacaggcat tgaatggcct
     1261 ttttcatcga tgtagaaaca ctcattattt gcattgaaaa tacgtatgat tggttcgcgt
     1321 tgcgatacct ccacatgcag tactccatca agtgttgaaa atacttccgc attctctatg
     1381 tatggatttg tttctaagat agtttccaac aaagccgtgt taatatcaac gatgtttttt
     1441 cctgtaggaa atttatcgct ctcacgaagt atatttatca catcgctgtt gtctataaat
     1501 cctttttgat cactatcaac aattttaatt tgcaactctt tg
//
LOCUS       DAORQA010000382         1518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_16788_length_1518_cov_7.589200, whole genome shotgun sequence.
ACCESSION   DAORQA010000382 DAORQA010000000
VERSION     DAORQA010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16788_length_1518_cov_7.589200"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..>1518)
                     /locus_tag="PK736_10080"
     CDS             complement(312..>1518)
                     /locus_tag="PK736_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRC33777.1"
                     /translation="LYIARKIIRGRKDKSLSNPVVNIAVLSVALSVAVMLIAVVSVQG
                     FKTEIARMVSGFAGHISIRSFDNNDSYEANAIYTDSVDVKKLRANTDIAAIQQIATKA
                     GILKSQTDMQGIALKGYGSDYNPEFFKNNLVEGRNIRFADSLTNDIIISSAISKQMNL
                     QCNDKVLCYFVQQPPRVRKFNIVGIYKTGLEEFDNTFVICDIRHIQKLNSWQPNEIGG
                     YEIFLRDFESIDKVTIAVNEASSFNIRAESIIEKFPQIFNWLNLINGNVIVILVLMII
                     IACVNMIATLLILILDNTNTIGMLKALGARNRMVRQIFLYIACYLTFLGIVIGNIIGL
                     GLAFIQHKFGLLKLPQETYYLDVAPIQFDWFLITGINLGAFVLCILFLLIPIAWIARV
                     EPVKVIRFQ"
BASE COUNT          495 a          279 c          279 g          465 t
ORIGIN      
        1 tgcctcttaa aaaaaaataa cgagctaagt attccttaag ttgacgtgta tgccttacag
       61 gacaaaaaac aggtgggtgc tcaggcagtt accaagcgat agtccctaag ggacatgcgt
      121 aagttaaaat aataatttta gtttaaaata taaggtaagc tcattttgaa ataacgctaa
      181 actatagctg ggaaaatcta tcgcgtaagg cttaataaca gaggacattg ctctattata
      241 aatattggag tttatcgaac tactattttg aaactcgctc gctatttcaa caatgtactt
      301 gtaaaaaatc cttattgaaa ccttatcact tttaccggct ctacacgggc aatccatgca
      361 atgggaatta ataaaaataa aatgcataaa acgaatgcgc caaggttgat gcccgtaatt
      421 aaaaaccaat cgaattgaat gggtgcaaca tcaaggtaat atgtttcctg tggtagtttt
      481 aataaaccaa acttgtgttg aataaaagct aatccaagac cgataatatt acctatcacg
      541 attcccaaaa aagtaagata acatgctatg taaagaaata tttgtcgcac cattctgttg
      601 cgcgcgccca atgccttgag catgcctatg gtatttgtgt tgtcgagaat aagaataagt
      661 aaagttgcaa tcatgtttac acaggcaata attatcatca gtacaagaat aacaatcaca
      721 ttgccattga tgaggttgag ccagttaaat atttgcggaa atttttcgat gatagattct
      781 gcacgtatgt tgaatgacga tgcttcgttt acagcgatag ttactttatc aatactttca
      841 aaatcgcgga gaaatatttc gtaaccacct atctcatttg gttgccatga atttaatttt
      901 tgtatgtgtc ttatatcaca aataacaaat gtgttgtcaa actcttcgag gccggtttta
      961 taaattccaa caatattaaa tttacgaacc cgtggcggtt gttgcacaaa ataacacaac
     1021 accttatcgt tgcattgcaa gttcatttgt tttgatatag ctgatgaaat aataatatcg
     1081 ttggtaagcg aatcggcaaa gcgaatgttg cgtccttcca caagattgtt tttaaaaaat
     1141 tcgggattgt aatcgctgcc ataacctttg agtgcaatgc cttgcatatc ggtttgcgat
     1201 ttaagaatgc cggctttagt agctatttgt tgaattgcag caatatcggt atttgctctg
     1261 agttttttta catctacgct atcggtataa atggcattgg cctcatacga atcgttgtta
     1321 tcgaaactac gtatagaaat atgtccggca aagccactta ccattcgggc aatctcggtt
     1381 ttgaaaccct gcacgcttac aactgcaatc agcataactg caacactgag cgctacactt
     1441 agcacagcaa tattcaccac gggattggaa agagatttat ccttgcggcc acgaataatt
     1501 ttacgcgcta tatataat
//
LOCUS       DAORQA010000383         1517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_16819_length_1517_cov_3.519152, whole genome shotgun sequence.
ACCESSION   DAORQA010000383 DAORQA010000000
VERSION     DAORQA010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16819_length_1517_cov_3.519152"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1517
                     /locus_tag="PK736_10085"
     CDS             <1..>1517
                     /locus_tag="PK736_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33778.1"
                     /translation="NLIYTGRTDFQVKVRGYRIELGEIENAINSFDNISGSAVITRPD
                     STGQNNLYAYVVAQNNFAKAQLNSGKGKDQQYIKQNVDDDYNVALNEIKRFSHLQLRS
                     LLKYGLHDNIAVMGGDHILLIPKIQNTYKHYTAVCIDKEEYAATKKFVANENLSQTTT
                     VQFIDDFISQKDGSSVFDTFIIDGESLYFNSANSFIDFISVVTKLLKPDGQFIILNVR
                     SNALEHTRLLFHELEHQRIDATIGRINQKVNELHQSKKGLRIDPEFFEQNKAAFNGFE
                     YLQYVANDVPTTDESKYFYNVIACKPLQIKYEGPTRVLNYKKDIGNKLRINHALSEHI
                     YEVKIITDIPCEHLLQEAEARNLVELANANSILKDLKVLYQSYGEGVTYAELKKLADS
                     REYYLKLNFSSSQRDAKFDAVFMPYAVAQDYANFNIEFNAAETEDVSFTNVEEENIHE
                     DFPFQELKHHLESKLPAYMIPQHYMLLEQFLLTPAGKIDRKAFPEIKSVAKRKNKT"
BASE COUNT          527 a          264 c          280 g          446 t
ORIGIN      
        1 aaccttattt acacaggtcg tacggacttt caggttaaag tgcgtggtta tagaatcgaa
       61 cttggcgaaa tagaaaatgc catcaactca tttgataata taagtggcag tgcagtaatt
      121 acgcggcccg acagtacagg gcaaaataat ttgtatgctt atgtagttgc gcaaaataat
      181 tttgccaaag cacaacttaa ttcaggaaaa ggtaaagacc aacaatacat aaagcaaaat
      241 gtggacgatg attacaatgt tgcactgaac gaaataaaaa gattttccca tcttcaattg
      301 cgcagtttat taaaatatgg tttgcatgat aatattgcgg taatgggagg cgaccacatt
      361 ttattgatac ctaaaattca gaatacctat aagcattaca cagcagtttg tattgataaa
      421 gaggaatatg ctgcaaccaa aaaatttgta gccaacgaaa atctttcaca aactactacc
      481 gtacagttta ttgatgattt tatttcgcaa aaagatggct catcggtatt cgacacattt
      541 attattgatg gcgaaagttt atattttaat tctgccaatt cttttatcga ttttatttcg
      601 gtagtaacaa aattgctaaa gcccgatggt caatttatta ttcttaatgt gcgcagcaac
      661 gcccttgaac atacacgttt gttgtttcat gagttggaac atcaacgcat agatgctacc
      721 attggccgta taaatcaaaa agtaaatgaa cttcatcagt ctaaaaaggg attgagaata
      781 gaccctgaat tttttgaaca aaataaggct gcattcaatg gattcgaata tttgcaatac
      841 gttgccaacg atgttcctac aactgatgaa tcgaaatatt tttacaatgt tattgcctgc
      901 aagcctctgc aaataaaata cgaagggcca acaagggtat tgaattacaa aaaagacata
      961 ggcaataaac tgcgcatcaa tcatgcttta tcggaacata tttatgaagt aaaaataatt
     1021 accgatattc catgtgagca tcttttgcag gaagccgaag ctcgaaattt ggtagagctt
     1081 gcaaatgcaa acagcatatt aaaagattta aaagtgcttt atcaaagcta tggcgaaggt
     1141 gtaacctatg ccgagttgaa aaagcttgcc gactcgcgcg aatattattt gaaacttaat
     1201 tttagcagtt cgcagcgcga tgcaaagttc gatgcggttt ttatgccata tgctgttgcg
     1261 caggattatg caaacttcaa tattgaattt aatgctgccg aaaccgaaga tgtttctttt
     1321 accaatgttg aagaagagaa tattcacgag gactttccat ttcaggaatt gaaacaccac
     1381 ctcgaaagca aattacctgc ttatatgatt cctcagcact atatgttgct tgaacaattt
     1441 ttgcttacac ctgccggaaa aatagatcgt aaagcatttc ccgaaattaa gagtgttgca
     1501 aaacgtaaaa ataaaac
//
LOCUS       DAORQA010000384         1511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_16895_length_1511_cov_8.232143, whole genome shotgun sequence.
ACCESSION   DAORQA010000384 DAORQA010000000
VERSION     DAORQA010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16895_length_1511_cov_8.232143"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1338)
                     /gene="clpX"
                     /locus_tag="PK736_10090"
     CDS             complement(85..1338)
                     /gene="clpX"
                     /locus_tag="PK736_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRC33779.1"
                     /translation="MKNKTVNDDKCSFCGRDKSNVRLLIAGESGNICDECVGHANGLL
                     KDETTALGKKPQNAPTSLLKPMEIKAYLDQYVIGQEHAKKVLSVAVYNHYKRLSHKMK
                     TKNDVELDKSNIIMLGETGTGKTLLARTIARILNVPFCIADATVITEAGYVGEDVESV
                     LSRLLQAADYDVALAEKGIVYIDEIDKIARKSDNPSITRDVSGEGVQQGLLKLLESSI
                     VNVPPYGGRKHPEQKMISVNTESILFICGGAFVGIDKIINRRLRSNALGFNKSNEAEI
                     HDKENVYKYVAPSDLKNYGLIPELIGRLPIITALEPLDKEALRNILTEPKNALTKQYK
                     QLFQMEKVNLEFDNDALDYIVEKAIEFKLGARGLRSLCETIMLDAMYEMPSQKPTTKD
                     SNFNITLSFAKSKISKSDLHRLKIA"
BASE COUNT          397 a          314 c          293 g          507 t
ORIGIN      
        1 cactatacgt catggcgagt gcaacgaagc aatcccggca tcgcacagac ttagcaaagt
       61 agagattgct tcgaaaaaaa accgttaagc aatttttaat ctgtgcaaat cgcttttcga
      121 tattttgctt ttggcaaaac ttaaagtgat gttgaaattc gaatctttgg tggtaggttt
      181 ttgagatggc atttcataca tggcatcgag cataatagtc tcgcacaacg agcgcaaacc
      241 acgggcgccc aatttaaatt ctatggcttt ttctacaatg taatcaagtg catcattatc
      301 aaattcgagg ttcacctttt ccatttgaaa caattgcttg tattgttttg tcaatgcatt
      361 ttttggttcg gtgagtatgt tgcgcaatgc ttctttatcg agaggttcca gtgcagtaat
      421 aattggtaag cgtcctatga gttcgggaat gaggccatag ttttttaaat cggaaggggc
      481 aacatattta tatacatttt ctttgtcgtg tatttcggct tcgttgcttt tattaaatcc
      541 aagcgcattg gagcgcaacc tgcgattgat aattttatcg atacccacaa acgcacctcc
      601 gcatatgaat aaaatacttt cggtgtttac cgaaatcatt ttttgttcag gatgttttcg
      661 acctccatag ggtggcacat tcactatcga actttcgagc aacttgagta atccttgctg
      721 tacaccttcg ccacttacat cgcgggtaat ggatggattg tcgcttttac gtgctatttt
      781 atctatttcg tcaatgtata caattccttt ttcggcaagg gcaacatcat aatcggcagc
      841 ttgcaacaat cgcgatagca cactctccac atcttcgcct acgtagccgg cttcggtaat
      901 cacagtagca tcggcaatgc aaaatggcac gttgagtatg cgtgcaatgg tgcgtgccaa
      961 cagggttttg cctgtacctg tttcaccaag cataatgatg ttcgacttat cgagttcaac
     1021 atcatttttg gttttcattt tatggctgag tcgtttgtag tgattataca ctgccaccga
     1081 taatactttt tttgcatgct cttgcccaat cacatactga tcgaggtagg cctttatttc
     1141 cattggcttt agtaacgatg tgggcgcatt ttgcggcttt ttgccaaggg ccgtagtctc
     1201 atctttaagt aacccattgg catggcccac gcattcatca catatatttc ccgactcacc
     1261 ggcaatcaac aatcgcacat tacttttatc gcgtccgcaa aagctgcatt tgtcatcgtt
     1321 cactgtttta tttttcatct ttggtaatgt tatgctttaa tgcatgtttg cataaaaagc
     1381 aatttgtttt ctgtgtacaa tatttttcgc aaagaaaacc attttaagcc gaataggaaa
     1441 acaaatcgtg ttttggaatg aatctgtgct ttaaaaatga gataatatca catcctattc
     1501 cattaataat c
//
LOCUS       DAORQA010000385         1494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_17121_length_1494_cov_8.801251, whole genome shotgun sequence.
ACCESSION   DAORQA010000385 DAORQA010000000
VERSION     DAORQA010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17121_length_1494_cov_8.801251"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..>1494)
                     /locus_tag="PK736_10095"
     CDS             complement(66..>1494)
                     /locus_tag="PK736_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRC33780.1"
                     /translation="TTTDSVVLVFEWQYKIATDMDSHREGVIDSASFFIAYFFPRIAV
                     YDDVFGWNNTQYNDQVEFYNDFCNFDYTITVPQNYQVWATGDWLNANEILDKQTLKKY
                     NAAMSSTSEVNIFDSLYYFQKSKDNRQTTKQNAFKFKASNISDIAFAVSSHYYWRATS
                     VDVKGKKVFTSVVYNSRNISYANILDMASKSVIYFANEWPGTGFPFSHMTLFNGYGQM
                     EFPMMCNDVHNENVDDDITLTTHEISHTYFPFEMGINETRFAWMDEGWAVFFEYMASV
                     NAFKLKDSTATYPAYYFKKYIASKSLDLDVPMMAPSDVLFEKSYGFNSYGKPAAALFA
                     LKAMLGDSLFKICLQKYMSDWRGKHPQPWDFFFSFNNTSKRNLNWFWQNWYYDWGRTD
                     WAITDVEKQTNSETIITLENIGGKIFPVDIYISTADTTTVYHHVPQTNARIFTITATG
                     NDITKVQLKFPMIPDVNKLNDVWEK"
BASE COUNT          449 a          286 c          280 g          479 t
ORIGIN      
        1 atagtgaaat aagcaaaatt ttatttactt aacgataaca attttaaatt caaaaatgca
       61 acgcatcact tctcccacac atcatttaat ttatttacat caggaatcat cggaaatttc
      121 aactgcactt ttgtaatatc gttgcctgtg gctgtaatgg taaatatacg ggcattggtt
      181 tgtggtacgt gatgataaac agttgtagtg tcggctgttg aaatgtaaat atctacaggg
      241 aaaattttgc cacctatatt ttcaagagta attattgttt cgctgtttgt ttgtttttct
      301 acatcggtaa ttgcccaatc ggtgcggccc caatcgtaat accagttttg ccaaaaccaa
      361 tttaagttgc gtttgctggt attattaaat gagaagaaaa aatcccaggg ctgcggatgt
      421 ttgccccgcc aatcgctcat atatttttgg aggcatattt taaacagcga atcgccaagc
      481 atggccttga gcgcaaatag tgccgcggca ggtttgccat atgaattaaa accatacgat
      541 ttttcgaaca acacatcgga aggtgccatc ataggtacat cgaggtcgag tgatttactt
      601 gcgatatatt ttttgaaata atatgccgga taagttgccg tgctatcttt caatttaaat
      661 gcgtttaccg atgccatata ttcgaaaaaa actgcccatc cttcatccat ccatgcaaag
      721 cgtgtttcgt ttattcccat ttcgaaagga aaatatgtat ggcttatttc gtgtgtggtg
      781 agggtgatat cgtcatccac attttcgtta tgcacatcgt tacacatcat aggaaattcc
      841 atttgtccgt agccattaaa caaagtcatg tgcgaaaatg gaaagccggt gccgggccac
      901 tcgttagcaa aataaattac cgacttgctt gccatatcaa gaatgttggc gtacgatata
      961 ttcctagaat tgtacaccac acttgtaaaa acttttttac cttttacatc aaccgaagtt
     1021 gcacgccaat agtaatgtga cgacaccgca aaagcaatat cgctaatatt ggatgcttta
     1081 aatttaaatg cattttgttt ggttgtttgc ctgttatctt ttgatttttg aaaatagtat
     1141 aacgagtcga aaatattaac ctcgcttgtt gacgacatgg cagcattata ttttttcaaa
     1201 gtttgcttat cgagtatttc gtttgcatta agccaatcgc ctgttgccca aacttgataa
     1261 ttttgcggaa cagtaatagt gtaatcgaaa ttgcaaaagt cgttgtaaaa ttctacctga
     1321 tcgttatatt gagtattgtt ccaaccaaac acatcatcgt acacggctat ccgtggaaaa
     1381 aagtaagcaa taaaaaacga tgcggaatca attacacctt cgcggtgcga atccatgtcg
     1441 gttgcaattt tatactgcca ttcaaaaaca agcaccaccg aatcggttgt tgtt
//
LOCUS       DAORQA010000386         1489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_17188_length_1489_cov_4.861227, whole genome shotgun sequence.
ACCESSION   DAORQA010000386 DAORQA010000000
VERSION     DAORQA010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17188_length_1489_cov_4.861227"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..511
                     /locus_tag="PK736_10100"
     CDS             143..511
                     /locus_tag="PK736_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33781.1"
                     /translation="MKIFLSFLLTSAIILQSMSSLIIVAGYKMNKAYITENFCENIDE
                     PDMCCEGMCHVEKQLKAESDKAESPNSTLKEKQETVQFFQSNKIFAFASCENRPTNIF
                     FYLQILLSKNSADIFHPPSV"
     gene            642..>1489
                     /locus_tag="PK736_10105"
     CDS             642..>1489
                     /locus_tag="PK736_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33782.1"
                     /translation="MKNIFILLISMCFCHMLIACDFCNCYLGLNPQYKKNIVGLRTHY
                     KLYNGTHHDLAEFSDAALTNKDFWEKRTEVELHAQWYPVQKLQIIFSLPYIYNIEGMS
                     APALLAFGNHHHDNENENNSAGPISGFGDPLIIAQYQLFNKTNSDTNKLSHRLFAGGG
                     VKFPLGKYKLGDNADPLDRTHQPGSGSFDFIASATYLAKIKRAGFNVNVSYLQSTMNN
                     ESFQFGNRFNANAIFYYQCNISKTLFFPNIGSFVEQSQKDWTNNYYIELPTTPATSGP
                     VLIPMR"
BASE COUNT          506 a          279 c          256 g          448 t
ORIGIN      
        1 tgcaaaaata gcactacagt atttgcaaat tgtataaata acgagcgaat gttatatcat
       61 tttaagctaa tggaattttt tttttagaaa cgttgaaaag taaataaaga aagtttactt
      121 ttgccgcgta aataaaaaca cattgaaaat attcctctct tttttactta cttcagctat
      181 cattctgcaa tcaatgagca gtttgataat tgtggcagga tacaagatga ataaggctta
      241 cattaccgaa aacttttgcg aaaatataga tgaacccgat atgtgttgtg aaggaatgtg
      301 tcacgttgaa aaacaattga aagccgaaag cgacaaagca gaatcaccca attccacttt
      361 aaaggaaaaa caagaaactg tacaattttt tcaaagtaac aaaatatttg cttttgcatc
      421 ctgtgaaaac cgaccaacta atattttttt ctatttacaa attctactgt ctaaaaacag
      481 tgccgacatt ttccatccac cctccgttta atatttttta tagaattacc acatcaattt
      541 cgtgtgtggt gaattattgt tgttttcatt tattcaaaat gaaccaatga taatatgcgt
      601 gtaacgtaac tttaaagcta tataaaatat aaaacttaat aatgaaaaat atattcattc
      661 ttcttataag tatgtgtttt tgtcacatgc ttatagcgtg cgatttttgc aattgctatt
      721 tgggattaaa tccacagtac aaaaaaaata ttgttgggtt gcgtactcat tataaattgt
      781 acaacggcac acatcatgat ttggctgagt ttagcgatgc tgcattaacc aacaaagatt
      841 tttgggaaaa gcgcaccgaa gttgagctcc atgcgcaatg gtacccggtt caaaaactac
      901 agattatttt ttcgttgcca tacatatata atatcgaagg catgagtgct cctgcgctat
      961 tggcctttgg caaccatcac cacgacaacg aaaatgaaaa caatagtgcc gggccaataa
     1021 gtggatttgg cgacccattg ataatagcac aataccagtt gtttaataaa acaaatagcg
     1081 acaccaacaa attatcgcac cgactgtttg ccggtggcgg ggtaaaattt ccattgggca
     1141 agtataaact aggcgacaat gccgatccgc tcgatagaac gcaccaaccc ggctcgggca
     1201 gtttcgattt tattgcaagt gcaacctatc tcgcaaaaat aaagcgtgcg ggtttcaatg
     1261 taaatgtgtc gtatctgcaa tcaacaatga acaacgaaag ttttcagttt ggaaaccgct
     1321 ttaatgcgaa tgccattttt tactatcaat gcaatattag taaaacacta tttttcccta
     1381 atattggaag cttcgttgag cagtcgcaaa aagattggac caataattac tacatcgaac
     1441 tacctacaac ccctgccacc agcggccctg tgttgatacc aatgcgaat
//
LOCUS       DAORQA010000387         1486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_17230_length_1486_cov_4.739343, whole genome shotgun sequence.
ACCESSION   DAORQA010000387 DAORQA010000000
VERSION     DAORQA010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17230_length_1486_cov_4.739343"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1486
                     /locus_tag="PK736_10110"
     CDS             <1..>1486
                     /locus_tag="PK736_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRC33783.1"
                     /translation="YFISGKIGDGIFLASAIVLVATISLYQDSRSRNALKKLKVFTQP
                     TCKVIRNGEVTEIMSDEIVIGDCLMTEEGTSIAADGIIIHSNDFSVNESILTGESLTI
                     YKDKNSNDNAVFRGTTVASGLAIVEVKSIGNQTRIGKIGKSIENIAEEKTPLELQINN
                     FVKKMVLAGAIVFAVVWVLNYLRSHNALDSLLKALTLAMSILPEEIPVAFTTFMALGA
                     WRLMKMGIVVKQMKTVETLGSATVICTDKTGTITENKMSLAKLFVWPSQKITDAGAML
                     TDDEKSLIELAMWASEPIPFDAMEVALHHSYFTNAAIDNRPQYKMVHEYPLAGKPPMM
                     THLHENELKQRIIAAKGAPEALMSISNLSVDEKTQIEKAIHTMASDGYRILGVAEAVF
                     DGYNFPKQQQEFSFVFKGLVAFYDPPKKNINKVLQDFYTAGIAVKIITGDNALTTSAI
                     AKQINFVGHEKNISGDELMKLSDDELNKCVTSTNVFTRMFPEAKL"
BASE COUNT          489 a          274 c          312 g          411 t
ORIGIN      
        1 atattttata agcggcaaaa taggcgatgg aatatttctg gcttcggcca ttgtgcttgt
       61 tgcaaccatt tcattgtatc aggattcgcg tagtcgaaat gctttgaaaa aactaaaagt
      121 gtttacgcag cctacttgca aggttattcg caatggcgaa gtaaccgaaa ttatgagcga
      181 tgaaatagtg attggcgatt gccttatgac agaagaagga acatcaattg cggccgatgg
      241 cattattatt cattcaaatg atttttcggt aaacgaatct attctcacgg gcgaatcgtt
      301 aactatttat aaagacaaaa attcaaatga caatgctgtt tttcgtggca ctaccgttgc
      361 aagcggactt gccattgttg aagtgaaatc cattggcaac caaacccgca ttggaaaaat
      421 tggaaaaagc attgagaata tagccgaaga aaaaacacca ttggaattgc aaataaataa
      481 ttttgtaaag aagatggtgc ttgccggagc cattgttttt gcagttgtat gggtcttaaa
      541 ttatttgcgt tcgcacaatg cgctcgatag tttattaaaa gcacttactc ttgctatgag
      601 tatattgccc gaagaaattc ctgtagcctt taccactttt atggcgctgg gcgcttggcg
      661 attgatgaaa atgggtatag tagtaaagca aatgaaaacg gtagaaacac ttggtagtgc
      721 taccgtaatt tgtaccgata aaacaggcac cattaccgaa aacaaaatga gcctggcaaa
      781 actatttgta tggccctcgc aaaaaattac cgatgccggt gcaatgctca ccgatgatga
      841 aaaatcgcta attgaacttg ccatgtgggc cagcgaaccc attcctttcg atgcaatgga
      901 agtggctttg catcattcct attttacaaa tgctgccatc gataatcgtc cacaatataa
      961 aatggtacac gagtatccgc ttgcaggtaa gcctcccatg atgacacatt tgcacgaaaa
     1021 cgaattgaaa caacgcatca ttgcagctaa aggcgcaccc gaagcattga tgagtatttc
     1081 caatttatcg gttgatgaaa aaacgcaaat agaaaaagct attcatacaa tggcaagtga
     1141 tggctacagg attttaggcg tagccgaagc ggttttcgat gggtacaatt ttcccaaaca
     1201 gcaacaggaa tttagttttg ttttcaaagg gcttgttgca ttttacgatc ctcccaaaaa
     1261 aaatatcaat aaggtattgc aagattttta tactgccggc attgcagtaa aaattataac
     1321 aggcgataat gctttaacca caagtgcaat tgcaaagcag ataaattttg tgggccacga
     1381 aaaaaatatt tcgggcgatg aactcatgaa actaagcgat gacgaattaa ataaatgcgt
     1441 aacgagtaca aacgtattta ctcggatgtt tcctgaggca aaatta
//
LOCUS       DAORQA010000388         1463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_17563_length_1463_cov_4.601562, whole genome shotgun sequence.
ACCESSION   DAORQA010000388 DAORQA010000000
VERSION     DAORQA010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17563_length_1463_cov_4.601562"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>768)
                     /locus_tag="PK736_10115"
     CDS             complement(<1..>768)
                     /locus_tag="PK736_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33784.1"
                     /translation="KIQLALIMLGGIIILAFAVGRPLPQSITFIRGVDYQQPFNFYYG
                     PMIDKEIAKDDNETAMKIMKKFMELEPDDVREMNATSPKLNDYQLVLARVYQDFHFKY
                     SKLSERAGDIQTAKNEDARATELQEIVVLNDNTGSIESALKKAQLAPPGTKEILYRNQ
                     VAPLLNEIKLNPNNTKPYRALNEVFINLNIPDSSIYVLQQLLKLNSDNFYAVFQLGTV
                     YGKYKNDLNNSALYLEKAFAMEPRNEDVCINLGIVYS"
     assembly_gap    769..784
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<785..1428)
                     /locus_tag="PK736_10120"
     CDS             complement(<785..1428)
                     /locus_tag="PK736_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33785.1"
                     /translation="MLFLFLTITISFFVFRNEPIHFLRYSGILLGGALLAYSPLLIRN
                     AIVGVPLFTQNSIGSIAVIASNDVKYDPASSYALNVKNTTEIIDKTGGKTLPSLVDAI
                     QTHPSVMSYITLLAKKLALVFHWYEFPNNKNFYYYRIHAPVLNYTFVNLIFIAPLAFI
                     GIILSIMQRRKLWALYLLLCMHLFLLVAFLVLSRYRIPFEAALIPFSGFAIAEL"
BASE COUNT          496 a          260 c          271 g          420 t
ORIGIN      
        1 atactgtaaa caatgccaag gtttatacat acatcttcgt tgcgcggttc catggcaaag
       61 gctttttcaa gataaagcgc agaattgttc aagtcatttt tgtatttacc atacacggtt
      121 cctaattgaa aaacagcata aaaattatcg gagttgagtt ttaataattg ctgtaaaaca
      181 taaatgcttg agtcgggtat attcaaattt ataaaaactt cgttcaaagc cctgtaaggt
      241 tttgtattgt taggattcaa ttttatttca tttaacaaag gagcaacctg gttgcgatat
      301 aaaatttctt tggtgccggg aggagcaagc tgtgcctttt tgagggcact ttcaatgctt
      361 cccgtattat cattcaatac tacaatttct tgtaattcgg ttgcacgggc atcttcattt
      421 tttgcagttt gaatgtcacc tgcacgttcg ctcaatttag aatatttaaa atgaaaatcc
      481 tgatacactc ttgccagcac caattgataa tcattaagct taggagaagt agcattcatt
      541 tctcttacat catcaggttc aagctccata aattttttca taattttcat ggcagtttcg
      601 ttatcgtctt ttgctatctc tttgtcaatc ataggcccgt aataaaaatt aaacggttgc
      661 tgataatcga ctccccggat aaatgtgata ctttggggca aaggccgccc aactgcaaat
      721 gcaagaatga taatgccgcc caacatgata agggcaagtt gtatttttnn nnnnnnnnnn
      781 nnnnatcagt tcagcgatag caaatcccga aaaaggaatc aaagccgctt caaatggaat
      841 acgatacctc gacaaaacca gaaatgccac gagcagaaaa agatgcatac aaagtaacaa
      901 atacagggcc cataattttc tacgttgcat tattgaaagt ataataccaa taaatgcaag
      961 aggcgcaata aaaataagat tgacaaaagt gtaatttaat accggtgcat gtatacggta
     1021 gtaataaaaa tttttgttgt tgggaaattc gtaccaatga aaaacaaggg cgagcttctt
     1081 tgcaagcaat gtgatataac tcatcacgct gggatgtgtt tgaatagcat cgactaacga
     1141 aggcaaagtt ttaccacccg ttttatcaat aatttctgtt gtatttttta cattcaatgc
     1201 gtagctgctg gcagggtcat acttaacatc attggaggca atcaccgcaa tggaaccaat
     1261 actattttgt gtaaacaaag gcacacccac aatggcattg cgaataagca aaggtgaata
     1321 agccagcaat gcaccaccaa gtaaaatacc ggagtatcgc aaaaaatgaa tgggttcatt
     1381 gcggaaaaca aaaaacgaaa tggtaatagt aaggaataaa aaaagcacaa aaactgattt
     1441 gagtaatatg caaagtgcaa gaa
//
LOCUS       DAORQA010000389         1450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_17733_length_1450_cov_5.668100, whole genome shotgun sequence.
ACCESSION   DAORQA010000389 DAORQA010000000
VERSION     DAORQA010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17733_length_1450_cov_5.668100"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PK736_10125"
     CDS             complement(<1..747)
                     /locus_tag="PK736_10125"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HRC33786.1"
                     /translation="MSTLQCANTILMIRPKHFGYNLETSVTNKFQNLGNVDNEKVLAE
                     FDDVYYKLIQSGIEVIVYDDKDELVTPDSVFSNNWLHVDHGGNIFLFPMLAPNRRIEK
                     RNDIVDDIATKFAIEKIEDWSRYEKEGSFLESTGSMVFDHANRIAYASISARTDEQLF
                     LKFCGYNNYKPVTFRTFDNAGFEVYHTNVIMSIGDEFCVLCDECFLNASERELVKFNL
                     KSCEKKIILITQAQMENYCANILQVQNKFGE"
     gene            1158..>1450
                     /locus_tag="PK736_10130"
     CDS             1158..>1450
                     /locus_tag="PK736_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33787.1"
                     /translation="MSIGNHFKEDKILAEVVEEPIIEKKEPRKRKQKTPESETSTEEK
                     KETTTEAQPKQRRILGLTFMLLSVVFFLSILSYAISFTHDFDKVAGLGWWQVF"
BASE COUNT          493 a          276 c          223 g          458 t
ORIGIN      
        1 ttcgccaaac ttattttgca cttgcaaaat gtttgcacaa taattttcca tttgggcttg
       61 ggtaataaga atgatttttt tttcgcacga tttcaaatta aattttacca actcgcgttc
      121 acttgcattt aaaaagcatt catcgcatag cacacaaaat tcatcgccaa tgctcataat
      181 aacgttggtg tggtacactt cgaaaccggc attatcgaat gtgcgaaaag taacaggctt
      241 ataattattg tatccgcaaa atttcaaaaa caattgttca tctgttcggg ccgaaatgct
      301 tgcataagca attctgttgg cgtggtcgaa aaccatactt ccagtactct ccaaaaaact
      361 tccctccttt tcgtatcgcg accaatcttc aattttttca atcgcaaatt ttgtagcgat
      421 gtcatcaact atatcattac gtttttctat tcttctattt ggagcaagca tagggaataa
      481 aaaaatattt cctccatggt ctacatgcag ccagttattc gaaaaaacag aatcgggagt
      541 taccaattcg tccttatcat catacacaat tacttcaata ccgctttgta ttaatttata
      601 atatacatca tcaaactcgg caagtacttt ttcattgtca acattgccaa ggttttgaaa
      661 tttatttgta accgaagttt ctaaattata accaaaatgt tttggccgta tcattaaaat
      721 tgtattggca cattgaagtg tagacatagc gaaattttat cgtttgagag gcgtaaatct
      781 aaacaaacaa attgaaggtt tgcatcaggg tttacaattg ttttttttga aacactctcg
      841 ccttgaatat aaatcgattt caaattacag cattcgtcat tcaatctaaa tgcataaaac
      901 aataatatgc agccaaaaga gttttgctat aataatccga tatactaaaa cttgtgttat
      961 tcaaatacct aaatggctct ggcattttgc aatggttatc aacacgtcca cctcaacatg
     1021 cagcaatagc aacacttttg gccaattggt gtgttgttta taataaactt aaacaaagcc
     1081 atttcaacca aatcgatatg gcacatcatg gttaaattta cgaagcacat aaaccttatt
     1141 caaaaactat aaaaaaaatg tcgataggaa atcattttaa agaagacaaa attttagccg
     1201 aggttgtaga agaacccatc atagaaaaaa aagaacctcg aaagcgaaaa caaaaaacgc
     1261 ccgaaagtga aacttcaacc gaagaaaaaa aagaaaccac aactgaggca caaccaaaac
     1321 aaaggcgcat tctgggtctt acttttatgc tcctgtctgt tgttttcttt ttatctattc
     1381 ttagttatgc gattagtttc actcacgatt tcgataaagt tgcaggcctt ggttggtggc
     1441 aagtatttag
//
LOCUS       DAORQA010000390         1436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_17940_length_1436_cov_3.857350, whole genome shotgun sequence.
ACCESSION   DAORQA010000390 DAORQA010000000
VERSION     DAORQA010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17940_length_1436_cov_3.857350"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..276)
                     /locus_tag="PK736_10135"
     CDS             complement(22..276)
                     /locus_tag="PK736_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33788.1"
                     /translation="MKIIILGISITAGLFLLLACTQTTNANKNNTATQTEKLMPNLSL
                     TDTSLTKVVKTPEEWKKLLSPFEFYVLREQGTEPAFQNAY"
     assembly_gap    293..324
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<325..960)
                     /gene="msrA"
                     /locus_tag="PK736_10140"
     CDS             complement(<325..960)
                     /gene="msrA"
                     /locus_tag="PK736_10140"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRC33789.1"
                     /translation="MKSILKMKYFNNVGVMFCMIVMCSCNSTAQKPTTPAAAPQQIPA
                     PQGRAIAVFAEGCFWCSEHIFEAVAGVDSAVSGYSGGKTVNPTYEHVCSETTGHAEAV
                     MVYYNPKEVSYEELVKVFFSSQDPTTPNQQGPDRGSSYRSIAFYQNNMERETIQTEID
                     IVNKSKRFRNEVVTEVLPFEKFYRAEEYHQDYIEHHPENGYVQNVSLPRFKL"
     gene            complement(1095..>1436)
                     /locus_tag="PK736_10145"
     CDS             complement(1095..>1436)
                     /locus_tag="PK736_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRC33790.1"
                     /translation="IMLQTLFFKFTASAESVYIFTALGIEPFGRIGSGIAELIASILL
                     LINRTTLLGAIMGMGIMAGAMVSHILVLGLVVMDDGGQLFAYALITFICCALLTYINK
                     EKIPALLKFKI"
BASE COUNT          412 a          264 c          256 g          472 t
ORIGIN      
        1 atgtccttct ttgtgattat tctaatatgc attttggaat gccggctcag taccttgttc
       61 acgcaaaaca tagaattcga atggcgacaa taactttttc cattcctcgg gcgtcttcac
      121 cacttttgta agtgacgtat cggtaagtga taaattcggc attaattttt ctgtttgagt
      181 tgcagtatta tttttatttg cgtttgttgt ttgtgtgcat gccaacaata gaaacaaacc
      241 tgcggttatt gatattccca aaattattat tttcattatt atttatttta atnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnntaattt gaagcgcggt agcgaaacat tttgaacgta
      361 cccgttttca ggatgatgct caatatagtc ctgatgatac tcttcggcac gataaaattt
      421 ttcgaatggt aaaacttctg ttacaacttc atttctaaat cgtttcgatt tatttacaat
      481 gtctatttct gtttgaatcg tttcgcgttc catattattt tgataaaatg caatggagcg
      541 ataagaagaa cctcggtcgg ggccttgttg atttggcgtt gtaggatctt gcgagctgaa
      601 aaaaacttta accagctcct catagctcac ttctttagga ttgtagtaaa ccattactgc
      661 ttcggcatgc cccgttgttt cgctgcaaac atgttcataa gtagggttaa cagttttacc
      721 tccggagtaa cccgaaacag cactgtcaac accggcaaca gcttcgaaaa tatgttcact
      781 acaccaaaag cacccttctg caaaaacggc tatggctctg ccttgtggtg ccggtatttg
      841 ttgtggtgcc gctgcaggtg ttgttggttt ttgtgctgtt gaattgcatg aacacattac
      901 tatcatacaa aacataactc caacgttgtt aaagtatttc attttcaaaa tggatttcat
      961 tttcatatta ttttatgatt acaacaaacg tacgctacga aagttggttt agatttataa
     1021 taataaatag acttaagagt agagagtgat gaaaacataa aatgcaacta ttctttaaag
     1081 caaaagtttc tgatttaaat tttaaatttt agcagcgcag gaattttttc tttatttatg
     1141 taagttaata atgcacaaca aataaatgtg ataagtgcat aagcaaatag ttgaccgcca
     1201 tcgtccatca ctaccaatcc caaaactaaa atgtgtgaaa ccattgcccc agccattatc
     1261 cccattccca ttattgcgcc taacagtgta gtgcgattta tcagcagcaa tatagatgct
     1321 attaattcgg caatgcccga acctatgcgg ccaaatggtt cgatgccaag tgcggtgaat
     1381 atataaaccg actcggcact tgcggtaaat ttgaagaata gagtttgcag cattat
//
LOCUS       DAORQA010000391         1423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_18114_length_1423_cov_5.410088, whole genome shotgun sequence.
ACCESSION   DAORQA010000391 DAORQA010000000
VERSION     DAORQA010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18114_length_1423_cov_5.410088"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PK736_10150"
     CDS             <1..714
                     /locus_tag="PK736_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC33791.1"
                     /translation="AYKFASHGYNLIINGRREDRLNQIEDDIKSNFDVKVLSLPFDVR
                     NRKEVENAINSMPYEFKQIDILVNSAGLASGLSYIQDGNIDDWETMIDTNVKGLLYVS
                     RAIMPTMVARKKGHIINIASLAGKETYAKGNIYCATKQAVDSISRAMRIDMLPYGIKV
                     TNIAPGAAETEFSLVRFHGDAEKAAKVYQGYEPLHAEDVADIVFFAASRPAHVVMNDI
                     VITPLAQANTAYLVRNQEA"
     gene            810..>1423
                     /locus_tag="PK736_10155"
     CDS             810..>1423
                     /locus_tag="PK736_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33792.1"
                     /translation="MKQKLLTKLLLTLVVVLCGCNYDRISDQPKIENTSVTAPMSQVA
                     SQVVAVCYCKPDQSKDATNKCFSKKLNLRKYALMAMMDTKQYLTKMQNDQHSNISSCC
                     DTNVTADCGINSTDNPIDTLLRNIPNATTFDEESLIIFAASEGKPFSRDLIVKYKTKE
                     GLWLMTLQMQDYVDANSSASSQLSKMPDYKCMYVSSDKNQPMFY"
BASE COUNT          466 a          262 c          288 g          407 t
ORIGIN      
        1 gcatataaat ttgcatcgca tggctataat ttaattatta atggtcgcag agaagacaga
       61 ctcaaccaaa ttgaagacga cataaaatcg aacttcgatg tgaaggtttt gagcttgccc
      121 ttcgatgtgc gcaatcgtaa agaggttgaa aatgccatca actctatgcc ttatgagttt
      181 aagcaaatag atattttggt gaatagtgca ggacttgcaa gtggtctttc ttatattcag
      241 gatggcaata ttgacgattg ggaaaccatg atagatacaa atgtgaaagg gcttctttac
      301 gtatctcgtg ctataatgcc aactatggtt gcacgtaaaa aaggacatat cataaacata
      361 gcatcgcttg caggcaaaga aacttacgcc aagggaaaca tatattgcgc aacaaaacaa
      421 gcggtggatt ctatatcgcg tgctatgaga attgatatgc tgccttatgg aataaaagta
      481 accaacattg ctccgggagc tgccgaaaca gaattttcgc tcgtgcgctt ccatggtgat
      541 gccgaaaaag ccgcaaaagt atatcagggt tacgagcctt tgcatgccga agatgttgcc
      601 gatatagtat tctttgctgc atcgcgccct gcacatgtag tgatgaacga tatagtgata
      661 actcctctgg ctcaggcaaa tacggcctat ttggttagaa accaggaagc ataaaatcaa
      721 aaaagttttg tcaagattgt gttaaattac gtattttaac ttaaagtaaa gataatttgc
      781 ttttcttttg gccttaaata aaaatcaaga tgaaacaaaa attgctcaca aaattgttat
      841 tgaccttggt tgttgtactg tgtggttgca attatgatcg cattagcgac caaccaaaaa
      901 tagaaaatac ttcagttact gcaccaatgt cgcaggtagc atctcaagtg gtggcagtgt
      961 gctactgtaa gcccgaccaa agtaaagacg cgacaaacaa atgcttttcg aaaaagctaa
     1021 atttgcgtaa atatgcttta atggctatga tggataccaa acagtatctt acaaaaatgc
     1081 aaaacgacca acattcaaat atttcatctt gctgcgatac taatgtaact gcagattgcg
     1141 gaatcaattc tactgataac cctatcgata cattgttgcg taatattcct aatgccacca
     1201 catttgacga ggaaagcctt atcatttttg cggccagcga gggtaaacct ttttcgcgcg
     1261 atttgattgt aaagtataaa accaaagaag gcctgtggtt gatgacttta cagatgcagg
     1321 attatgttga tgcaaattct tcggcatcat cacaactatc aaagatgccc gattataaat
     1381 gcatgtatgt ttcttccgat aaaaatcagc cgatgtttta tta
//
LOCUS       DAORQA010000392         1417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_18203_length_1417_cov_8.763583, whole genome shotgun sequence.
ACCESSION   DAORQA010000392 DAORQA010000000
VERSION     DAORQA010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18203_length_1417_cov_8.763583"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1317
                     /locus_tag="PK736_10160"
     CDS             181..1317
                     /locus_tag="PK736_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HRC33793.1"
                     /translation="MIPLSVPNINGNEWKYVKDCLDTGWISAVGSYVDKFEQTMAAYT
                     GAKYAVAVANGTCALHLSCLLMNIQQGDGVIVPDITFIASVNSIKQCGAEPILMDVNE
                     QNWQMDLQLLESFLSTDCEIINGICAIKKSKRKIKAIMPVHVLGHMCNMQKLKSIAIK
                     FNLKVIEDATEALGSTYKSKHAGTMGDIGCLSFNGNKIISTGGGGVILTNNKEIAKLA
                     KHLSTQAKTSPEEYIHDANGFNYRMVNILAAIGMAQMEQLPSFLHKKKDIAEQYHKKL
                     NGVGDIQFIETEKNVVPNNWLFTIRTKKRKALFQHLKNSGVQSRPLWQPMHMLAIHSK
                     HLFVTKNNISTILYNDCLSIPCSTDLKIKDQSIVIKKVVEFFST"
BASE COUNT          526 a          261 c          256 g          374 t
ORIGIN      
        1 tcccaacaac agcgaagtag aacgattgct gggcagcgct gtaaaacttt taaaaattac
       61 tggttggcaa ccaacaacaa aactcgaaac aggaattgaa caaaccataa attggtttac
      121 caataaagat aatttacggt tgtataaaca caatcaatac aacgtataaa caaaaaaagc
      181 atgatacctt tatcagtacc caacataaac ggcaacgaat ggaagtacgt aaaagactgc
      241 ctcgatacag gttggatatc ggctgtggga agctacgtag ataaatttga gcaaaccatg
      301 gccgcataca ccggagcaaa atatgctgtg gcagtagcca acggcacttg tgctttgcac
      361 ctgtcgtgtt tgcttatgaa tatacaacaa ggcgatggtg tgatagtgcc cgacatcact
      421 tttattgcca gtgtaaattc gataaagcaa tgtggtgccg aacctatatt gatggatgta
      481 aatgaacaaa actggcaaat ggatttacaa ttgctcgaaa gttttttgag caccgattgc
      541 gaaataatca atggtatttg cgccatcaaa aaatcgaaac gaaaaataaa agctataatg
      601 cctgttcatg ttttaggaca catgtgcaac atgcaaaaat taaaatctat tgcaataaaa
      661 ttcaatttga aagtaattga agatgctacc gaagcacttg gcagcacata caaaagcaag
      721 catgccggca caatgggcga tattggttgc ttaagtttca atggaaataa aattatttcg
      781 acaggtggcg gtggtgttat tcttactaac aataaagaaa ttgcaaagct tgctaaacac
      841 ctaagtactc aggcaaaaac gagccccgaa gaatacatac acgatgccaa cggttttaat
      901 tatcgaatgg taaatattct tgctgcaata ggcatggcac aaatggaaca actcccttcc
      961 ttcttacata agaaaaaaga tattgctgaa caatatcaca aaaaattgaa tggtgttggc
     1021 gatattcaat ttattgaaac cgaaaaaaat gttgttccaa ataattggtt gtttaccatt
     1081 cgcacaaaaa aacgaaaagc attgttccaa catctaaaaa actcaggagt gcaatcccgc
     1141 cctttgtggc aacccatgca catgcttgct attcacagta aacatttatt tgtgactaag
     1201 aataacatat cgacaatcct ttacaacgac tgtttgagta taccatgttc taccgatttg
     1261 aaaattaaag accaaagtat agttattaaa aaagtagtag aatttttcag cacttaaaaa
     1321 acaatctatg cgtgtattat tgataaccga acccaggcgt attgacttat atcaatattt
     1381 acaacacgac acaaaaaacg aatatattat tctttgg
//
LOCUS       DAORQA010000393         1411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_18310_length_1411_cov_3.440265, whole genome shotgun sequence.
ACCESSION   DAORQA010000393 DAORQA010000000
VERSION     DAORQA010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18310_length_1411_cov_3.440265"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PK736_10165"
     CDS             <1..434
                     /locus_tag="PK736_10165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRC33794.1"
                     /translation="HAIKEKLIEKNKEIFKAEVETIEGYIMRHELKMIKTPSGLRYHK
                     MIESKGDSIISGDKVKVAYSVYLLDGTHCYSSDSTGLLTFKVGEYDVPNGFHEIALLL
                     KVGEKAQLIIPSHLAYGITGDNNKIPYASALNCFIEIKERN"
     gene            451..1317
                     /locus_tag="PK736_10170"
     CDS             451..1317
                     /locus_tag="PK736_10170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRC33795.1"
                     /translation="MNLKKVSFYVIATTVVAVNACKKPDFQTNANGLQYKIFAAGKSE
                     VKPKKEDIVEMHYRYTTMSDSTLYESTKESVDPIVTQMPEPPFKGSFQEGMMLMSPGD
                     SAVMIASADSFFAKIGQALPAYFKKGDKMKMFVKVYSIQSLEAFQTKLKTKEVTERNE
                     YIAKNNITTPADASGLISIIQDPGTGMSPAAGDTAVVNYTGKLLNGKVFDSSEGKEPI
                     SFPLGVGRVIPGWDMGLMKLKEGGKATLIIPSDLAYGPQGSGPIPPASTLVFDVQLLK
                     VITKYRKPYSKN"
BASE COUNT          505 a          250 c          285 g          371 t
ORIGIN      
        1 aacatgctat caaagaaaaa ttgatagaaa aaaataaaga gatttttaag gccgaagtag
       61 aaaccataga aggctatatt atgcgtcacg agctaaaaat gataaaaaca ccaagcggac
      121 tgcggtacca taaaatgata gaatctaaag gcgatagtat tataagtggt gataaagtga
      181 aagttgctta cagtgtttat ttactggatg gaacgcattg ttacagcagc gatagcaccg
      241 gtttgctaac atttaaagtg ggcgaatacg atgtgccgaa tgggtttcac gaaatagcat
      301 tattgttgaa agttggcgag aaagcacaat taattatacc ttcgcacctc gcttacggca
      361 tcactggcga taataataag attccctatg ccagcgcact caattgtttt attgaaataa
      421 aagaaagaaa ttaaaattat taatattgtt atgaatttaa aaaaagtaag tttttatgta
      481 attgcaacta cggtggtcgc agttaatgct tgtaaaaagc cggattttca aaccaatgcc
      541 aatggattgc aatacaaaat atttgcagcc ggcaagagtg aagtaaagcc taaaaaagaa
      601 gatattgtag aaatgcatta tcgctataca acaatgagcg attctacttt gtatgaatca
      661 acaaaagaat cggtcgatcc tattgtgact caaatgcccg aaccaccttt caaaggctcg
      721 tttcaagaag gtatgatgct gatgtcgcca ggcgatagtg ctgtaatgat tgccagtgcc
      781 gattcgtttt ttgcaaaaat cggtcaggcc cttccggcat attttaagaa aggcgataag
      841 atgaaaatgt ttgtaaaagt ctattcaata caatcactcg aagcatttca aacaaaacta
      901 aaaaccaaag aagtaacaga gcggaatgaa tatattgcta aaaacaatat caccacgcct
      961 gccgatgcaa gcggattgat atcaattatt caggaccccg gaacaggcat gtcgcctgcg
     1021 gctggagata ctgcagttgt aaactacacc ggcaaattac tcaatggaaa agtgtttgat
     1081 tcatcggaag gcaaagaacc tatttcattt cctcttggtg tgggccgtgt tataccaggt
     1141 tgggacatgg gcttaatgaa actaaaagaa ggtggcaaag caacactaat aataccatct
     1201 gatttagcat atggcccaca aggaagcggc cctatccctc cagcttctac tttggttttc
     1261 gatgtgcaat tgttgaaagt tataaccaaa tatcggaaac catactcaaa aaactgaacg
     1321 acaatacagc aaataagttg aattcgattt ttgataacaa agcataacgc gtaattgatt
     1381 atactcgcac cctataattg cagaaaatgg a
//
LOCUS       DAORQA010000394         1409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_18324_length_1409_cov_104.771049, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000394 DAORQA010000000
VERSION     DAORQA010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18324_length_1409_cov_104.771049"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="PK736_10175"
     CDS             <1..1305
                     /locus_tag="PK736_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HRC33796.1"
                     /translation="LERECTRNIEMQWLCGKLIPSYHSIADFRKDNPKALRNTFKLFV
                     SFLKDIDLIGGELIAIDGTKIRAHNSKKNNYSQKKIERHFTFIENKISEYMAQLDKCD
                     AAEDEIKITNITKKIEQLAARKIFYETLETELKESGDTQISTTDADARALLIQGQVVE
                     VAYNTQAAVDDKHKLVAATHTINRNDRNALTDIATEAKQNMDVSTYTAIVDKGYHNGR
                     QIQQATNAGIVTIVAPPEIVNSNEHGTTPDYVVTKFVYDESTDTYTCPQGATLTTTGT
                     WHRKSRERDTYQFKKYRTPKCKTCPAKHLCTGRAKGGREIERSEFAAEVEANNARYNS
                     HKETYRKRQEINEHIFGTIKRVWGYYYTNLKGLEKVNGEWSLIMTVYNIKRTINILGI
                     QPLLEKLKNWNPDYTRISRLKSKTTALHQFLRIQFHEHSVAA"
BASE COUNT          530 a          327 c          278 g          274 t
ORIGIN      
        1 cttgagcgcg agtgcacgcg caacatcgaa atgcaatggc tctgtggcaa gttgataccg
       61 agctaccata gcattgccga ttttcgtaag gacaacccaa aggcattgcg caatacgttc
      121 aagttatttg taagttttct taaagacatt gacttaatag gaggagagct tattgcaata
      181 gatgggacca aaatacgcgc ccacaacagc aagaaaaata attattcgca gaaaaaaatt
      241 gagcgccact ttacattcat tgaaaataaa ataagtgaat atatggcaca attggataaa
      301 tgcgatgcag ccgaagacga gataaaaatc acaaacatta ccaaaaagat tgagcaactt
      361 gcagcgcgaa aaatatttta tgaaacactg gaaacagaat taaaagaaag tggcgacacg
      421 cagataagca ccaccgatgc cgatgcacgc gcattgctca tacaaggcca ggtagtagaa
      481 gtagcctaca acacacaagc agccgttgac gataagcaca aacttgtagc tgccacacac
      541 accataaacc gtaacgacag aaacgccctt accgatattg ccaccgaagc aaagcaaaat
      601 atggatgtat caacgtatac agctatagta gataagggat accacaacgg ccggcaaata
      661 cagcaggcca ccaatgccgg catagtaacc atagtagcac cacccgaaat agtaaacagc
      721 aacgagcatg gcaccacccc cgattacgta gtaactaagt ttgtatacga tgaaagcacc
      781 gatacatata catgtccaca aggcgcaaca ctaacaacca caggcacatg gcatcgcaaa
      841 tcgcgcgagc gcgatactta ccaatttaaa aaataccgta caccaaaatg taaaacctgc
      901 cctgcaaaac acctgtgcac agggcgcgcc aaaggaggcc gcgaaataga acgcagtgag
      961 tttgctgccg aagttgaagc caacaatgct cgctataaca gccacaagga aacataccgc
     1021 aagcgacaag aaataaacga acacatattt ggtacaataa aacgagtatg gggatattac
     1081 tacacaaact tgaaaggatt agaaaaagta aatggcgaat ggagcctcat catgacagtt
     1141 tacaacatca agcgcacaat caacatacta ggtatacagc cactgttaga gaaactaaaa
     1201 aactggaatc ctgactacac aaggatttca cggctaaaat caaaaacgac cgcattacat
     1261 caatttctac gcattcaatt tcacgagcat agtgtagcag cgtaaaaaac aacccccgtt
     1321 aaaaacgcca caaatcgtat atggtcaccg ttacgaagct gtttcggccg ccaaaatttt
     1381 taaaaggtag gttttttcac atcctgacg
//
LOCUS       DAORQA010000395         1402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_18448_length_1402_cov_5.146251, whole genome shotgun sequence.
ACCESSION   DAORQA010000395 DAORQA010000000
VERSION     DAORQA010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18448_length_1402_cov_5.146251"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1022)
                     /locus_tag="PK736_10180"
     CDS             complement(108..1022)
                     /locus_tag="PK736_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC33797.1"
                     /translation="MKHTILVTGSNGLLGQKIIYAALNNPEINLIATAIGANRTKEKS
                     GYTYEELDITDEQRIDFLFEKYKPNTLINTAASTNVDLCEKNREQAKLLNADAVAFMA
                     KACKKHNTHFIHLSTDFVFDGTQGPYDELAEPNPLSYYAQTKFDGEKILMQSDIAWSI
                     LRTIIIYGTVDDGQRSNVVLWTKKSLEQHQKINVITDQYRCPTLAEDLADACIQCALK
                     KAEGLFHVSGKDFMSIWQAAEITADFFHLDKSLMQPVTTAQLNQPAKRPLVTGFVIDK
                     AKRILNYNPHSFTEGLEIVAGQLNKTEQ"
     gene            complement(1069..>1402)
                     /locus_tag="PK736_10185"
     CDS             complement(1069..>1402)
                     /locus_tag="PK736_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRC33798.1"
                     /translation="PFVENAIIHGLTQDNDVGLLTISFKQHDGKLACIIDDDGIGIEA
                     SLQNKKEKIKHNAVGMEVTRERISALNRMSTEKILLEVEDRKNIDGTSGTRVKIIFPN
                     AFATEKRN"
BASE COUNT          363 a          267 c          258 g          514 t
ORIGIN      
        1 aattgaagaa tccgatgaaa atgcacaaaa atatgttggc acttgtatca ccataatgct
       61 tccgcttatc atcaacgatg aacttattca acattgatgg ggatattcta ttgctcagtt
      121 ttatttaatt gccccgcaac aatttctagc ccttctgtaa atgagtgcgg attgtaattt
      181 aaaatgcgct ttgctttatc aataacaaaa cctgtaacaa gaggacgttt agccggttga
      241 ttcaattgtg cagtggtaac aggctgcatc aacgatttat cgagatgaaa aaaatcggca
      301 gtaatttctg cagcttgcca gatgctcata aaatcttttc cactcacatg aaaaagtcct
      361 tccgcctttt taagcgcaca ttgtatacat gcatcggcaa ggtcttcggc aagtgtgggg
      421 cagcgatatt gatcggtaat cacatttatt ttttgatgtt gctcaagtga tttttttgtc
      481 cacagtacca cattgcttcg ctgtccgtca tccactgtgc cataaataat aatggtgcgc
      541 agtattgacc atgcaatatc actttgcatt aatatttttt cgccatcgaa ttttgtttgt
      601 gcatagtagc taagcggatt tggttcagca agttcatcgt acggcccctg tgtgccatcg
      661 aaaacaaaat cggtagagag gtgaataaaa tgtgtgttat gttttttgca tgctttggcc
      721 ataaaggcaa cagcatcggc attcaataat tttgcttgct cgcggttttt ttcgcacagg
      781 tctacatttg tcgatgctgc ggtgtttatg agtgtattgg gtttatattt ttcgaacaaa
      841 aaatctatac gttgctcatc agtaatatct agttcctcat acgtatatcc acttttttct
      901 tttgtgcggt tagcaccaat tgcagtggct attaaattta tttcaggatt gttaagtgct
      961 gcataaatta ttttttggcc aagtaagcca ttacttcctg ttactaaaat ggtgtgcttc
     1021 atagtttgct tttctttttc gcgaaaaata ggttattcat ttattttttt aatttctttt
     1081 ttcagtcgca aaagcattgg gaaaaataat ttttacgcgt gtgcccgatg tgccatcgat
     1141 gttttttctg tcttccactt ccaataatat tttttcggta ctcattcggt ttagtgcact
     1201 tatgcgttct ctagttactt ccattcctac tgcattgtgt tttatttttt cttttttgtt
     1261 ttgcaacgat gcttctatac ctataccatc gtcatcaatt atgcacgcta attttccgtc
     1321 atgttgttta aacgaaatag ttaacaaccc aacgtcatta tcttgtgtga ggccatgtat
     1381 gatggcattt tcaacaaagg gc
//
LOCUS       DAORQA010000396         1395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_18565_length_1395_cov_3.149254, whole genome shotgun sequence.
ACCESSION   DAORQA010000396 DAORQA010000000
VERSION     DAORQA010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18565_length_1395_cov_3.149254"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="PK736_10190"
     CDS             complement(<1..1383)
                     /locus_tag="PK736_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRC33799.1"
                     /translation="MAFPPSDAQTKTLQYKQSNVHDFGWVADKRFHVLKGEVELPNTK
                     RKVTLWAMFTPLEDHLWKNSIEYMHDAAYYYSLWNGDYPYNQITAVDGTIAAGGGMEY
                     PNMTIIGQSRTAHLLEMVIVHEIGHNWFYGILGSNERDHGWMDEGINSFNEMRYVYTK
                     YPIEKFDNRNELSALGKFSHWLHVDKLSDKQVRMGFYKVPALRKTSQAIETKSPDFTS
                     TNYARKNYTKKHLDYHLGTEMFDKCMQTYFETWKFKHPYPNDIKNVFMKMSDQNLAWF
                     FDSYIPQDINIDFKLKKVKLNPDQTYTVYVDNKNLPGYPYNIITYKKGKQVSNKWIWP
                     GKSETIVEAGDYDRIGIGTDEAIPDINMYNNAKSIKGIFKRGNKSKLRFLTGLDDGLS
                     KQLYWNPVAGYNLWDGTMLGASLHNFDSRPKKFNFHISPMYGFNSGSLAGFGFGSMNI
                     YSIKTADITID"
BASE COUNT          385 a          281 c          260 g          469 t
ORIGIN      
        1 gtctattgta atatctgccg ttttaatgct ataaatattc atcgaaccaa acccaaaccc
       61 agctaaggag ccactattaa aaccatacat tggcgaaata tggaaattga atttctttgg
      121 gcggctatca aagttatgaa ggcttgcacc aagcatagtg ccatcccata agttataccc
      181 tgcaaccgga ttccaataca gttgctttga taagccatcg tcaaggccag tcaaaaaacg
      241 gagtttactt ttattaccac gtttaaaaat gcccttaata ctttttgcat tgttatacat
      301 atttatatcg ggaattgctt catcggtgcc aataccaatt cggtcgtaat cgcctgcttc
      361 aacaatggtt tcggatttgc cgggccatat ccatttatta gaaacctgtt ttcctttttt
      421 gtaggttata atattgtaag gataaccggg caggttttta ttatcaacat atactgtata
      481 cgtttggtcg gggtttagct ttactttttt taatttgaaa tcgatattta tatcctgcgg
      541 gatgtagcta tcgaaaaacc atgcaagatt ttggtccgac attttcataa aaacattctt
      601 aatatcgttg ggatatggat gtttaaattt ccatgtttcg aaataggtct gcatacattt
      661 atcaaacatt tcggtaccca aatggtaatc caagtgtttt ttggtgtaat tttttctggc
      721 gtaatttgta gatgtaaaat cggggctttt ggtttcgatg gcctgcgaag ttttgcgcaa
      781 tgcaggaact ttataaaagc ccatacgtac ttgcttatcg ctcagtttat ccacatgcaa
      841 ccaatgagaa aatttcccta aggcacttaa ctcattacgg ttgtcaaatt tttcaatggg
      901 atacttcgta tatacatagc gcatttcgtt aaaggaattt atgccttcat ccatccaccc
      961 atgatcacgt tcgttagacc cgagtatgcc gtaaaaccag ttgtggccta tttcgtgtac
     1021 aatcaccatt tcgagcaaat gcgcagtgcg cgattgacct attatagtca tgttgggata
     1081 ttccattcca ccaccggcag caattgtgcc atcaaccgcg gtaatttgat tgtaaggata
     1141 atcgccattc catagtgaat aatagtatgc tgcatcgtgc atatactcta tgctgttttt
     1201 ccacagatgg tcttcgagtg gcgtaaacat ggcccacaaa gttactttac gtttggtgtt
     1261 tggtaattcc acttcacctt ttaatacatg aaagcgctta tcggccaccc aaccaaaatc
     1321 gtgaacattg ctttgtttgt attgcaaagt ttttgtttgg gcatcgcttg gcggaaatgc
     1381 catatcatat ttatc
//
LOCUS       DAORQA010000397         1393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_18595_length_1393_cov_5.074738, whole genome shotgun sequence.
ACCESSION   DAORQA010000397 DAORQA010000000
VERSION     DAORQA010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18595_length_1393_cov_5.074738"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1393)
                     /locus_tag="PK736_10195"
     CDS             complement(<1..>1393)
                     /locus_tag="PK736_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRC33800.1"
                     /translation="KEVYDFLASVSDKYGIGFWKPGAGIIHQVILENYAFPGGLMIGT
                     DSHTVNAGGLGMIAIGVGGADACDVMAGLPWELKMPKLIGVKLTGRLSGWASAKDVIL
                     KVAGILTVKGGTDKILEYFGDGAQAISCTGKGTICNMGAEIGATTSIFGYDKKMGDYL
                     RGTSRADIADMADGIAAHLCGDDEVYANPEKYFDEVIEINLSELEPHVNGPFTPDLAW
                     PLSKFAAAVREGGWPQQLSVGLIGSCTNSSYEDISRAASVAKQAADKGLVARSEFTIT
                     PGSEQVRYTVERDGFLETFTKIGGVVLANACGPCIGQWARHGAEKQEKNSIITSFNRN
                     FSKRADGNPNTHSFVASPEVVTAFAIAGDLTFNPATDYLTNDKGEKIKLDEPAGIELP
                     PTGFAVKDAGFQAPAEDGSKVEIKVSPTSDRLQLLAPFAAWEGVDLKGLKLLIKAKGK
                     CTTDHISMAGQRL"
BASE COUNT          344 a          365 c          280 g          404 t
ORIGIN      
        1 tttagccttt ggcctgccat gcttatatga tctgtagtgc attttccttt tgctttaatc
       61 aataatttca ggccctttaa atccacacct tcccaagctg caaatggtgc aagcaattgt
      121 aaacgatcgc tcgttggcga cacttttatt tctaccttac taccatcttc ggcaggggct
      181 tggaatccgg catcctttac ggcaaatcct gttggtggca actctatacc tgcaggctca
      241 tcgagtttta ttttttcgcc tttatcattt gtaagataat cggttgcggg attaaaagtc
      301 agatcaccgg caatggcaaa tgccgtaacc acttcggggc ttgccacaaa cgaatgtgta
      361 ttgggattgc catcggcacg cttactaaag ttacgattga aggaagtgat gatggaattt
      421 ttctcttgtt tctcggcacc atgtcgagcc cactgaccaa tgcaaggtcc gcaagcattt
      481 gccaacacca caccgccaat ttttgtaaac gtttcgagga atccatcgcg ctcaaccgta
      541 tagcgcacct gctcgctacc gggagtgatg gtaaattcac tgcgtgccac caaaccctta
      601 tcggcagctt gttttgcgac tgatgccgca cggcttatat cttcgtacga tgaatttgta
      661 catgatccaa ttaaacccac actcagttgt tgaggccagc caccttcgcg cacagcagca
      721 gcaaacttac ttagtggcca agctaaatcg ggcgtaaatg gaccattcac atgtggttcc
      781 agttcgctaa ggtttatttc tattacttcg tcaaaatatt tttcgggatt tgcatatacc
      841 tcatcatcgc cacaaaggtg ggcggcaatg ccatcggcca tgtcggcaat atcggcacgg
      901 ctggtaccac gcaggtagtc gcccattttt ttatcataac caaatataga agtggtagca
      961 ccaatttcgg cacccatgtt acaaatagtg cctttgccgg tgcaagaaat tgcctgtgcg
     1021 ccatcgccaa aatattcgag aattttatcg gtgcctcctt ttacggtaag tatgccggcc
     1081 acttttaata ttacatcttt tgctgatgcc caaccgctca aacggcctgt cagcttcact
     1141 cctataagtt ttggcatttt caattcccat ggcaatccag ccatcacatc gcatgcatcg
     1201 gcaccgccaa cgcctatagc aatcattccc aggcctccgg catttaccgt gtgtgagtcg
     1261 gtgcctatca tcaaccctcc cgggaaggca taattttcta atattacctg atgtataatt
     1321 cctgcaccgg gcttccaaaa acctatgcca tatttatcgc ttactgatgc aagaaaatca
     1381 tacacttctt tgt
//
LOCUS       DAORQA010000398         1382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_18758_length_1382_cov_4.506405, whole genome shotgun sequence.
ACCESSION   DAORQA010000398 DAORQA010000000
VERSION     DAORQA010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18758_length_1382_cov_4.506405"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1153
                     /locus_tag="PK736_10200"
     CDS             <1..>1153
                     /locus_tag="PK736_10200"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRC33801.1"
                     /translation="GIKDGDFTMVYGFPGRTQEYVPSVALDFIVNQVNPTRVNIRDKR
                     LEQINYGMQSNDTTRLQYSAKARSLANAYKKWKGEMIGINKSNAMEAKRVYEKKFSIW
                     VKENPERIKKYGMLLKDFDALYAKYKPYAKVQDYTNEAANAIEVLGLAGSFNNLIMLA
                     ENDTASEATWLAACKARAEGVKGFFKNYDTRVDKNVFIEMMCLYRDSIATDNLPNKVK
                     QWDNRTTITVYADRLFKKSAFVSQQKLLDLLVTGNKKKIVKLRNDEAFALFAEINNNY
                     VAYNREIAALQVEINKTMRAYMKALREYDVNKMFYPDANSTLRITYGNVKPYNPRNGV
                     IYLSSTTAAGIREKYNPADEDYATPAKLISLIDSKDYGTYAENGEIPDAF"
     assembly_gap    1154..1164
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1165..1355
                     /locus_tag="PK736_10205"
     CDS             <1165..1355
                     /locus_tag="PK736_10205"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRC33802.1"
                     /translation="VLNANGELIGTNYDREWEGIMSDMHYDVNLCRNISLDVRYTLFI
                     IDKFAGATNLIKEMKLVE"
BASE COUNT          488 a          222 c          289 g          372 t
ORIGIN      
        1 ggaataaagg atggcgactt tacaatggta tatggttttc ccggtcgcac acaagaatac
       61 gttccttctg ttgctttaga ttttattgtg aatcaggtaa accccacacg cgtaaatatt
      121 cgtgataagc gactcgaaca aattaactat ggcatgcaga gtaatgatac taccagattg
      181 caatattcgg caaaggcacg tagtctggcc aatgcataca aaaagtggaa aggtgaaatg
      241 attggaataa ataaatctaa tgccatggaa gccaagcggg tatacgaaaa aaaattttcc
      301 atttgggtaa aagaaaatcc cgagcgaatt aaaaaatacg gaatgctgct taaagatttt
      361 gatgcattat atgcaaagta taaaccatat gcaaaggtac aggattatac caatgaggct
      421 gcaaatgcta ttgaagtatt gggcctggca ggttcgttta ataatttaat tatgcttgcc
      481 gaaaatgata ctgccagtga agcgacatgg cttgcagcct gtaaagcgcg tgccgaagga
      541 gtgaaaggat tttttaaaaa ctacgatacg cgtgtcgaca aaaatgtgtt tatagaaatg
      601 atgtgcctgt atcgtgatag tattgcaacc gacaacttgc ccaacaaagt gaaacaatgg
      661 gataaccgaa caactataac tgtatacgca gatagattat ttaagaaatc agcttttgta
      721 tcgcagcaaa aattacttga cttgcttgtc acaggaaata aaaagaaaat tgtaaagctc
      781 cgaaacgatg aggcttttgc tttgtttgct gagattaaca ataattatgt tgcatacaat
      841 cgagaaattg cagcactaca ggtggagata aataaaacaa tgcgtgcgta tatgaaagca
      901 ttgcgggaat atgatgtgaa caaaatgttt taccctgatg caaattctac tttgagaatt
      961 acctatggaa atgtaaaacc ctacaaccct cgaaatggag taatatattt gagttcaaca
     1021 acagcagccg gcattcgcga aaaatataat ccggccgatg aagactacgc aactccggca
     1081 aaattaattt cacttattga tagtaaggac tatggaacat atgccgaaaa tggtgaaata
     1141 cccgatgcgt ttannnnnnn nnnnctgtac ttaatgccaa tggcgaactt attggtacaa
     1201 attacgatag agagtgggaa ggcattatga gtgatatgca ttatgatgta aacctatgcc
     1261 gaaacatttc gctcgatgtg cgttatactt tgtttataat agataagttt gcaggtgcta
     1321 ccaatttaat taaagaaatg aaattggttg agtaggaatt attaagaaac aaaagcctat
     1381 ac
//
LOCUS       DAORQA010000399         1377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_18831_length_1377_cov_9.920575, whole genome shotgun sequence.
ACCESSION   DAORQA010000399 DAORQA010000000
VERSION     DAORQA010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18831_length_1377_cov_9.920575"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="PK736_10210"
     CDS             <1..1073
                     /locus_tag="PK736_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33803.1"
                     /translation="ISSVSIGDAAGDAFGNTYLINAFGVGSQIKKLNSNAGVLWFQNN
                     NMAANKVEVGSDNNPVISGYPGATYGCAFMKYDSNGNMLWQNLDADGPNLSLLAHAQL
                     KLDGANAAYLAGSTMSQMGLCKVDSDGTSAWVATTSSGYPVCFDFGTDNSVYVTGGTT
                     ARYTQATPCNTPTGLFTINLSPVQAKFNWTKVPGAVQYEIWHKKVSASVWKKRFVNGI
                     AKAVVINNLTCNTPYEWKIRTLCDTVGVDVASAFSPVQNFTSASCRMNSADISTKEIL
                     FNVYPTIVDDFFTVETNGNSEIMNAQIVIADLTGRTVYNESINIDGTFTKQINVSNLP
                     SGLYFIKLIADNETYTSKIVIE"
BASE COUNT          477 a          239 c          264 g          397 t
ORIGIN      
        1 gtataagtag tgtttcgatt ggcgatgctg ccggtgatgc ttttggtaat acttatttaa
       61 taaatgcatt tggagtaggc agccaaataa aaaaattgaa ttccaatgcc ggtgtgttat
      121 ggtttcaaaa taataatatg gcggcaaaca aagtagaagt tggcagcgat aataatccgg
      181 taataagcgg atatccgggc gcaacatatg gttgtgcgtt tatgaaatat gatagtaatg
      241 gaaatatgtt gtggcaaaac ctcgatgccg atggtcccaa tctttcactg cttgcacatg
      301 cacaactaaa actcgatggt gccaatgctg cttatcttgc aggaagtaca atgtcgcaaa
      361 tgggattgtg taaagtggat agtgatggca catcagcctg ggtagctact acatcgagtg
      421 gctatcctgt atgtttcgat tttggcaccg acaatagcgt ttacgttacc ggaggcacta
      481 ccgcacgtta tactcaagcc actccttgca acactcctac aggattattc actataaact
      541 tatcgcctgt gcaggcaaaa tttaattgga ccaaagtacc cggtgcagtg caatatgaaa
      601 tatggcacaa aaaagtttcg gcatcggtat ggaaaaaaag atttgtaaac ggaattgcaa
      661 aagcggtggt gataaataac ctcacatgca atacaccata tgaatggaaa atacgaaccc
      721 tatgcgatac cgtgggcgtt gatgtggcat ctgcattttc acctgttcaa aattttacct
      781 ccgcttcttg tcgcatgaat agtgcagata taagtaccaa agaaattcta ttcaacgtat
      841 atccaaccat tgttgacgat tttttcactg tggaaacaaa tggaaattcc gaaattatga
      901 atgctcaaat tgttattgct gatttaaccg gaagaactgt atataacgaa tcaataaata
      961 ttgacggtac tttcacaaaa caaatcaatg tgagtaacct gccttctgga ttatatttta
     1021 taaagctaat agcagataac gaaacataca catcaaaaat agtaattgaa taaattacag
     1081 aaatataaca gatataaaat cgcaagatca atattgaata ttaaaactca acaataatat
     1141 acatgctgtt gcatgttgaa aaatatagct tgctcagcct gatggtggag aaaaacaata
     1201 ttgactaatt acatattttt cgcactgtgg aaagtgaaaa cgaatgatgc ttcaatagaa
     1261 taagcctttg ctttgaaaaa ttgaattcat tcgttttcac ttttaagctt atgtaacaaa
     1321 cgaagttatc agactaattc ctaaaataaa catttgcaaa ccctaggata taattaa
//
LOCUS       DAORQA010000400         1376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_18854_length_1376_cov_6.380772, whole genome shotgun sequence.
ACCESSION   DAORQA010000400 DAORQA010000000
VERSION     DAORQA010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18854_length_1376_cov_6.380772"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PK736_10215"
     CDS             complement(<1..511)
                     /locus_tag="PK736_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33804.1"
                     /translation="MNELEIVELDSAINNKSFEYYFQYKVPETAAGRLIYLTYIAYDI
                     DGNQGKNLQQIFVSDLTLKETAGLEFHSGFHFTNNAFDLETLLPATASLTDSTFRDLQ
                     SYITDSTIQDLPYYWKSPARGKFVRANNYNYALATLQTAESLYNASLPTDITDSLVVN
                     DIYIMKLGSM"
     gene            complement(729..1139)
                     /locus_tag="PK736_10220"
     CDS             complement(729..1139)
                     /locus_tag="PK736_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33805.1"
                     /translation="MKRILMILMVSGLLATTACTTYRTASVAAPVNVQVIIDYDNMDY
                     VGEVTGTATQSFALGLPIGGQRYRVAVASTAGGNILPGNNRGINNALYEALKQKPDAD
                     FLIPFSVQETRNVMFLGSKRTYTVKAKAFKIKAK"
     gene            complement(1185..>1376)
                     /locus_tag="PK736_10225"
     CDS             complement(1185..>1376)
                     /locus_tag="PK736_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33806.1"
                     /translation="AKWSMGGKTETEIDSKQGSTSTNLTYFSQEDDTNAYKDLKTSKF
                     GMDTNQNVRLTLNIYFNRN"
BASE COUNT          412 a          246 c          268 g          450 t
ORIGIN      
        1 ccatacttcc caacttcata atataaatgt catttacaac caacgaatcg gtgatatcgg
       61 taggtaagct tgcattgtat aaagactcgg cagtttgaag tgttgccaaa gcataattgt
      121 aattattggc tctaacaaat tttccgcgcg cgggcgattt ccagtaataa ggtagatcct
      181 ggattgtaga atcggtaatg tatgactgca aatcgcgaaa ggtagaatca gtcaaacttg
      241 cggttgcggg gagtaatgtt tcaaggtcaa aagcattgtt ggtaaaatgg aaacctgaat
      301 gaaattccaa accggcagtt tcttttaaag ttaaatcgct aacgaaaatt tgctgtaagt
      361 tttttccttg gttgccatct atatcatatg ctatgtatgt aagataaatc aacctgccgg
      421 cagcagtttc gggtactttg tattgaaaat agtactcgaa tgacttattg tttatggctg
      481 aatcaagttc aactatttcc aactcgttca ctatttgagt tttcaaaatg aactgtcgaa
      541 gttgtatttc agaagtaact tttatagaaa tactcacaat atcgccaggc tttgcatata
      601 atctgaaatc ggaaggggtt gaaagaacaa tggggtatac ctctttgtct ttcttacagg
      661 aagaaataaa aaagagacat accccaaaga taattgcgat gcgattaagc atgattggca
      721 agcgtgcctt atttagcttt tattttaaat gccttagctt ttacagtata ggtgcgcttg
      781 ctacccaaaa acattacatt tcttgtttct tgcacgctga atggaattaa gaaatcggca
      841 tctggctttt gttttaatgc ctcatataat gcattattta ttccacgatt gttacccggg
      901 agtatatttc caccggcagt agatgcaaca gctacacggt aacgttgccc tccaatagga
      961 agacccaacg cgaatgattg tgtagcagtg cctgttactt ctccaacgta gtccatatta
     1021 tcataatcga taataacttg tacgttaact ggagcagcaa cacttgcagt tcggtatgtg
     1081 gtgcaggctg ttgtggccaa caagcccgaa accattaata tcattagtat tcttttcatt
     1141 taattgaaat tttaaaaggt gaaaaatttt ctccggatgg aaaattagtt acggttgaag
     1201 taaatgttga gagttaatct aacattttgg tttgtatcca ttccaaactt acttgttttt
     1261 aaatctttgt aagcatttgt gtcatcttct tgcgagaaat aagtcaaatt agttgaggta
     1321 gaaccttgct ttgagtcaat ttccgtttca gtttttccac ccatcgacca tttagc
//
LOCUS       DAORQA010000401         1366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_19031_length_1366_cov_5.535469, whole genome shotgun sequence.
ACCESSION   DAORQA010000401 DAORQA010000000
VERSION     DAORQA010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19031_length_1366_cov_5.535469"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1366
                     /locus_tag="PK736_10230"
     CDS             <1..>1366
                     /locus_tag="PK736_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33807.1"
                     /translation="HSNSAQPIQFTNLSIDNHQLKLTCSSTIQVDKILLTQTSGPILG
                     NAITSCGSLNATITPSGSTTFCNGDSVQLSANNSMSSYLWSNGATTQSIVVKSAGNYS
                     VFITNYSGVSATSSIVGVIVNALPNANVNATFASICPPNTTELSVNSGVSYLWSTGAT
                     TKKIIVSATGAYIATVTNTAGCTRASVPFNVGLGNCGTTAQISPNGPITFCYGGTVIL
                     NCNSQSSYQWSTGSTAQSISVNQGGIYTVSVVNSFGIASSSSITITVNPVPYAQIVSS
                     GGDTVCEGYPLLLTAQGGLGYLWSNGESVATVLEEYAGTYTVTVTNEFACTSSASYII
                     HDGNCVPDATISFLGNPVFCEGDSCIFEAMPGMQSYLWSNGETTQTVVIKESIAALIL
                     TVTNINGYTTSSYPIETTMKAAPQAGIISTKDSLCAGQSAILSNMAPANSFYWSTGAT
                     TPNIQ"
BASE COUNT          424 a          245 c          275 g          422 t
ORIGIN      
        1 catagtaaca gtgcacaacc aattcagttt actaatttat caattgataa tcatcagcta
       61 aagttaactt gttcaagtac catacaagta gataaaatat tattgaccca aacgagtgga
      121 cctatattag gcaatgcaat tacgagttgt ggtagcttaa atgccactat cactccttca
      181 ggaagtacta ctttttgtaa tggcgatagt gttcaacttt cagccaataa ttcgatgagt
      241 agttatttat ggtctaatgg cgcaacaaca caaagcattg ttgtaaaatc ggcaggcaat
      301 tactcggtat ttattacaaa ttatagtggc gtatcggcta ctagcagtat tgttggtgta
      361 attgtaaatg cattacctaa tgccaacgta aatgcaactt ttgcttcaat ttgcccgcca
      421 aatactactg agctatccgt aaacagtggt gtaagctatc tttggtcaac aggtgcaact
      481 acaaagaaaa ttattgtgtc ggctacaggt gcatatattg caacagttac taatacagct
      541 ggttgcacac gtgcatcggt tccatttaat gtagggcttg gtaattgtgg caccaccgct
      601 caaattagcc ctaatggccc aataacattt tgttacgggg gtactgtaat tttaaattgc
      661 aactcacaaa gcagctatca atggtcaaca ggtagcactg ctcaaagtat aagtgttaac
      721 caaggtggca tctatacagt aagtgtagtt aatagttttg gcatagcatc atcgtcaagt
      781 attactataa cggttaaccc ggtgccttat gcccaaattg taagttcggg tggtgatacg
      841 gtttgtgaag gatatccgtt gctattaact gcgcaaggag gtttgggtta tttatggtcg
      901 aatggcgaaa gcgtagccac tgttttagaa gagtatgccg gaacctatac agttactgtt
      961 actaatgaat ttgcttgtac ttcaagtgcc agttatatta tacatgatgg taattgtgta
     1021 cctgatgcca caattagttt tttaggtaat cctgtatttt gtgaaggtga tagttgcatt
     1081 tttgaagcca tgcctggcat gcaaagttat ttatggagca atggagaaac aactcaaaca
     1141 gttgtaatta aggagagtat tgcagctctt atattaactg taaccaatat aaatggttat
     1201 acaacctctt cctatcctat tgagacaacc atgaaagcag caccacaagc aggtattatt
     1261 tcaacaaaag attcattatg tgcaggtcaa agtgctatac ttagtaatat ggctcctgca
     1321 aacagttttt attggagcac aggtgctact acaccaaata tacaag
//
LOCUS       DAORQA010000402         1365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_19043_length_1365_cov_6.243511, whole genome shotgun sequence.
ACCESSION   DAORQA010000402 DAORQA010000000
VERSION     DAORQA010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19043_length_1365_cov_6.243511"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /gene="rpsR"
                     /locus_tag="PK736_10235"
     CDS             <1..168
                     /gene="rpsR"
                     /locus_tag="PK736_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRC33808.1"
                     /translation="DYKDASFLFRFVNDQGKILPRRLTGTSLKYQRKVGQAVKRARHI
                     ALMPYVGDLLK"
     gene            199..648
                     /gene="rplI"
                     /locus_tag="PK736_10240"
     CDS             199..648
                     /gene="rplI"
                     /locus_tag="PK736_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRC33809.1"
                     /translation="MELILKQDVKNLGFANDVVKVRNGYGLNYLIPQGFAMIANATNK
                     KIHAEVVKQRAHKFAKIKDDAQSIIDSLSGIVLTIPMKAGESGKLFGSVTSQHLADLL
                     KKMGYTIDKKQISMPAEHVKALGTYSAEVVLHRDVKGPIQFEVIEQE"
     gene            747..1307
                     /gene="efp"
                     /locus_tag="PK736_10245"
     CDS             747..1307
                     /gene="efp"
                     /locus_tag="PK736_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRC33810.1"
                     /translation="MATTSDVSVGSILRYNGELCVVTEWQHRTPGNLRAFYQGKMRNI
                     KSGKSAENRFRAGEEVEIARVEFKEMEYLYEDNGALICMDNETFDQVPVPKEYFGDGM
                     SFMKEGDKVKVAFESDLPILGEPPTFVELMITYCEPGKKGDTATNTLKPATLETGAIV
                     SVPLFVNEGEKIKVDTRTSSYVERVK"
BASE COUNT          502 a          212 c          265 g          386 t
ORIGIN      
        1 gactataagg atgctagttt tttattccgt tttgtaaatg accaaggcaa aatattacct
       61 cgcagattaa ccggcacatc attgaaatat cagcgtaaag taggacaagc cgtaaagcgc
      121 gctcgtcata tagctttaat gccatacgtt ggcgatttgc taaaataatt ttttttcaaa
      181 aaataaaaaa ataaaaaaat ggaactcata ttaaaacaag atgttaagaa tcttggtttt
      241 gcaaacgatg ttgtaaaagt tcgaaacgga tatggattga attatcttat tccacaaggc
      301 tttgcaatga ttgctaatgc aactaataaa aaaatacatg ccgaagttgt gaagcaaaga
      361 gcacataaat tcgcaaaaat taaagatgat gctcaatcta tcatagattc attatcaggc
      421 atagtactta caattccaat gaaagcaggc gaatctggaa agctatttgg ttcagtaact
      481 tcgcaacatt tagcagattt actcaaaaaa atggggtata ccatcgataa aaaacaaata
      541 tcaatgcctg ctgaacatgt gaaagctttg ggaacgtatt cagccgaagt agttttacat
      601 cgtgatgtaa aaggtccaat tcagtttgaa gtaatagaac aagaataact tgaactccga
      661 aaattttatt aacagtgtta tattttacag atattaacac tgtttttttt tgaagatatt
      721 tttaaaaaca ggtaaaaaac aaataaatgg caacaacatc agacgtaagc gtaggcagta
      781 tactcagata taatggagag ttatgtgtgg ttaccgaatg gcaacacaga actcctggaa
      841 atctgcgcgc attttaccag ggcaagatgc gtaatatcaa atctggaaag tctgccgaaa
      901 atagatttcg tgcaggtgaa gaagttgaga ttgcacgcgt agagtttaaa gaaatggaat
      961 atttatatga agacaatggc gcattgattt gtatggataa cgaaacattt gaccaggtac
     1021 ctgtgcctaa agaatatttt ggtgatggta tgtcttttat gaaagaaggc gacaaagtaa
     1081 aggtggcctt cgaaagtgat ttacccatac ttggagaacc acctactttt gtagaattga
     1141 tgattactta ttgcgagccc ggaaaaaagg gcgataccgc aacaaatacc ctaaagccag
     1201 ccactcttga aacaggagcc attgtatcgg tcccattatt tgttaacgaa ggggagaaaa
     1261 taaaagttga taccagaaca agttcctatg ttgaaagagt aaaatagatt tatttaagtc
     1321 tcaaaagtat ttgcaatcag ttgcaataga tttaatatac ttcca
//
LOCUS       DAORQA010000403         1365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_19049_length_1365_cov_4.279389, whole genome shotgun sequence.
ACCESSION   DAORQA010000403 DAORQA010000000
VERSION     DAORQA010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19049_length_1365_cov_4.279389"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PK736_10250"
     CDS             complement(<1..881)
                     /locus_tag="PK736_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33811.1"
                     /translation="MQVIITGPATYISTVNGALTPIVNGDTLNYTIADFGLVDFNNDF
                     QIKIQTDTTAQANQQVCFEVNVTPTIGDNIPANNTLTHCFTVVNSYDPNLKSVYPINR
                     IDNADGKLTYTIQFQNTGNAPAQHIYVMDTLDAALDASTFELLAYSVQPQIQLIGNNL
                     RFNFPNINLPDSVNDEPNSHGYVQFSIKLKDSLPQGTQVANTSFIYFDFNPPVQTNTV
                     TDTLTDCDNLIANAWFAKTSLCKNDTLFAALELQYPYNNIKWYVDNVLSANGDSVAIS
                     NIGLGTHIIKVVIKTPY"
     gene            complement(989..>1365)
                     /locus_tag="PK736_10255"
     CDS             complement(989..>1365)
                     /locus_tag="PK736_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33812.1"
                     /translation="PLLWSFPICDTGNTNGCIPYWNISGKVYNDTNANCLAEINEPKY
                     NFIKLNLFRGSILEQTTYSNSEGNYAFNTDTGLFTITIDTSNKPFVVSCPLSFFDTAL
                     IASNETSHSHASPPSTLALQQH"
BASE COUNT          400 a          226 c          284 g          455 t
ORIGIN      
        1 cagtagggtg tttttattac cactttaatt atgtgtgtgc cgaggccaat attagaaatt
       61 gcaaccgaat caccatttgc agaaagtaca ttgtctacat accattttat attgttataa
      121 ggatattgca attccaatgc ggcaaacaaa gtatcgtttt tgcaaaggga agtttttgca
      181 aaccaagcat tggcaatcaa attatcgcaa tcggtaagtg tgtcggttac ggtattggtt
      241 tgtaccggag gattaaaatc gaaataaata aaacttgtat ttgctacttg tgtgccttgc
      301 ggtaaactat ctttcaactt tatactaaac tgcacatagc catgactatt gggttcgtca
      361 tttacgctat caggtaaatt gatatttgga aaattgaaac gtaaattatt tcctatcaat
      421 tgaatttgcg gttgcacgct atatgctaac aactcaaacg tgcttgcatc aagtgctgca
      481 tcaagcgtat ccatcacata tatgtgttgt gcaggtgcat tgcccgtatt ttgaaattgt
      541 atggtgtacg ttaatttgcc atcggcattg tcaattctat taattggata aaccgatttc
      601 aaatttgggt cataactatt tacaacagtg aagcaatgtg tgagtgtatt gttcgcagga
      661 atgttgtcgc ctatggttgg tgttacattt acttcaaaac aaacttgctg attggcttgt
      721 gcagtggtat cagtttgtat ttttatttga aagtcattat taaaatcaac caagccaaaa
      781 tctgcaattg tgtaattgag tgtatcgcca tttactattg gtgtgagtgc accattaacg
      841 gtgctaatat acgttgccgg gccggtgatg attacttgca ctgcaccgct tgtgcctaag
      901 gcacaatgaa ggccgtagtg attgcttaaa tctcctgcaa gtattttaag gttggcgaag
      961 ttggcagggc ggatgaggcc ggagtctctt agtgttgttg aagcgccaat gtcgaaggtg
     1021 ggcttgcatg agagtgcgaa gtttcgttac ttgctattaa agctgtgtca aaaaatgata
     1081 gtgggcaact aacgacaaaa ggtttgttcg atgtatcaat ggtaatagta aataatcccg
     1141 tgtcggtatt gaaagcgtag ttaccttctg agttggaata agttgtttgt tccaaaatac
     1201 tacctctaaa caaattcaat tttataaaat tatattttgg ttcatttatt tctgccaaac
     1261 aatttgcatt agtatcatta tatacttttc cactaatatt ccaataagga atacagccat
     1321 ttgtattgcc agtatcacaa atcggaaatg accaaagtaa tggga
//
LOCUS       DAORQA010000404         1355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_19206_length_1355_cov_10.586923, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000404 DAORQA010000000
VERSION     DAORQA010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19206_length_1355_cov_10.586923"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PK736_10260"
     CDS             <1..299
                     /locus_tag="PK736_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRC33813.1"
                     /translation="RVIAARQIQAERYKDTPEIFCNAQMNTKQLRSLCKLSEAGNSLL
                     KNAMDRLGLSARAYDRILKVARTIADLTNATDIGTEHLAEAIQYRSLDRDGWAG"
     gene            complement(543..>1355)
                     /locus_tag="PK736_10265"
     CDS             complement(543..>1355)
                     /locus_tag="PK736_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33814.1"
                     /translation="TAVSWKTAPAGTNIGAYLSTHFNAAFPSSLVVGGGCIGSKTLTL
                     SNPTAVRNFLNNQPLTGGATKLTVNLTNATGLTFPSAFGANLVALKMNVVFDAYDANF
                     APTSTVTLGSMIYQGGGTVNGLTVTQILNEANKLFGGCTSVYDSLEVRNAVKLINESW
                     LGSPGVPFNNVLTCPAPPKATNNALVDITSIVAYPNPTEGQLNIKFITEQEGNVNVRI
                     LDAIGRLVFNKSHAAVEGLNDFALDISNLNTGVYMVNVQVNGMQQTIRVVVK"
BASE COUNT          366 a          330 c          253 g          406 t
ORIGIN      
        1 ctcgcgtaat tgctgctcga caaattcaag ccgaacgata taaggatact cccgaaatat
       61 tctgcaacgc acaaatgaat acaaaacaac tgcgttcatt atgcaaactt tccgaagcgg
      121 gtaactcact tttaaaaaat gctatggatc gtctgggtct ttcggcacgt gcttacgacc
      181 gcattctcaa agtagcacgc accatagccg acctcacaaa cgccaccgac attggcaccg
      241 aacaccttgc cgaagccatt caataccgca gcctcgatcg cgatggatgg gcggggtagt
      301 tttattttta ttgcgggagg cggtggcctc tccggtatgt tgccgttcca gcaacataca
      361 atgcgcttcg cacccttcct ttttgcggct gcgtatacgg ttatggttag tttcgccttc
      421 tcttatgttt tcaaaaagaa ttcctaccaa caaaaaataa ttttacaaac acattgcact
      481 cttataaaac aaaaacgccc cacataatgc gaggcgtttt tgttattgta ctttagcgaa
      541 tattacttca ctactaccct tattgtttgt tgcattccgt tcacctgtac atttaccata
      601 taaactcctg tgttgaggtt gcttatgtct aaggcgaaat cgttcaggcc ttccactgct
      661 gcatgcgatt tattgaacac caaacgtcct attgcatcta agatacgcac attcacatta
      721 ccttcctgtt ctgtaatgaa cttaatattc aattgtcctt cggttggatt tggatatgct
      781 acaatgcttg taatatcaac caaagcattg tttgttgcct ttggtggtgc aggacaagta
      841 agcacattat tgaatggcac acctggactt cccaaccagc tttcgtttat cagtttcact
      901 gcattgcgca cctctaacga atcataaacg cttgtgcaac ctccaaacag tttattcgct
      961 tcgtttaata tttgagttac cgttaatcca ttaaccgtac caccaccctg ataaatcata
     1021 ctgccaaggg ttactgtact tgtcggagca aagtttgcat cataagcatc aaataccacg
     1081 ttcattttca atgccactaa gtttgcacca aatgcactcg ggaatgttaa gcctgttgca
     1141 ttagttaagt tgacagtcaa cttagttgcc ccgcctgtta aaggctgatt gttcaagaaa
     1201 ttacgtacag cagtaggatt tgataatgtg agcgttttac tacctataca accaccacct
     1261 acaaccagtg acgatgggaa tgctgcgttg aagtgtgtac ttaaataagc tcctatattc
     1321 gttccggcag gcgctgtttt ccacgatacc gctgt
//
LOCUS       DAORQA010000405         1354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_19226_length_1354_cov_6.577367, whole genome shotgun sequence.
ACCESSION   DAORQA010000405 DAORQA010000000
VERSION     DAORQA010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19226_length_1354_cov_6.577367"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>537)
                     /locus_tag="PK736_10270"
     CDS             complement(<1..>537)
                     /locus_tag="PK736_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRC33815.1"
                     /translation="LSRKIFAGFPSYSLGNLCAQLGITVENRHRAGGDADATAILFSM
                     LLQNDTEGHITQMKKKTNGEIYLPSHLNKEEFDKLPHSPGVYYFYDKDGKIIYIGKAK
                     NLRKRVASHFSNNSPRKRKQDFIKNITNISFKLCTTELMAIILESIEIKQHWPQYNRA
                     LKFNEKTYGVYDYCDQAGV"
     assembly_gap    538..547
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<548..887)
                     /locus_tag="PK736_10275"
     CDS             complement(<548..887)
                     /locus_tag="PK736_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRC33816.1"
                     /translation="MYAIVDIETTGGHASANGITEICILIHDGHKVIHRYDTLINPQI
                     SIPPFIQSYTNITNEMVSNAPVFGDIASDIYDLLHDKIFVAHSVNFDYTFIQHQLKQH
                     GFTLNAAKLCT"
     gene            1137..>1354
                     /locus_tag="PK736_10280"
     CDS             1137..>1354
                     /locus_tag="PK736_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRC33817.1"
                     /translation="MNIYIGNLDYKVSEAQLQELFSTYGAVSSAKIINDKFSGKSKGF
                     GFVEMPNDDEARNAMNALNGQELNSRAMT"
BASE COUNT          415 a          240 c          272 g          417 t
ORIGIN      
        1 accccggcct gatcgcaata gtcgtataca ccatatgttt tttcattaaa ctttagtgcg
       61 cgattatatt gtggccagtg ttgttttatc tctatcgatt cgagaataat tgccatcaat
      121 tcggtagtgc ataatttgaa agaaatattg gtgatgtttt ttataaaatc ttgtttgcgt
      181 tttcgtggcg agttattcga aaaatggctt gcaacacgtt tgcgtagatt ttttgcctta
      241 cctatataaa ttattttccc atccttatcg taaaaataat acactcccgg gctgtgtggc
      301 aatttatcga actcctcttt gtttaaatgt gacggaagat agatttcacc attagttttt
      361 ttcttcattt gggtgatgtg gccttcggta tcattttgca acagcatcga aaataaaatg
      421 gctgtggcat cagcatcgcc tcccgcacgg tgacggtttt cgacagtgat gcccagttgg
      481 gcgcaaaggt taccaagcga gtaagaagga aagccggcaa atatttttcg gctaaggnnn
      541 nnnnnnncag tgcaaagctt tgcagcattg agtgtaaagc catgctgctt caattgatgt
      601 tgtataaagg tataatcgaa attcacacta tgggccacaa atattttgtc gtgaagcaaa
      661 tcataaatat ctgaagcaat atcgccaaat acaggcgcat tactcaccat ttcgttagta
      721 atattggtat acgattgaat aaacggagga atagaaattt gcggattgat gagcgtatcg
      781 taacggtgaa ttactttatg gccatcgtgt attagaatac atatttcggt aattccattt
      841 gccgaggcat gaccacccgt ggtttctata tctactatgg cgtacatgat gtggtcttca
      901 aatggaagga ttattcaaaa tcaaagaaag aaaattttcg gtacaaaaat accataaagg
      961 gatggaaaca cgggctgaat cccaaaaata atctcacaaa aagcactttg ttttccttaa
     1021 agtcctttaa gaaggacttt tgccttcgtt tttagcccaa taataaaggt taaaataaat
     1081 ttgtttataa aaagtaaaag gttttctttg cactcgtatt aattaatttc tataagatga
     1141 acatttacat tggtaacctt gactacaagg taagcgaagc acagcttcaa gaattattta
     1201 gcacttacgg agccgtaagt tcagctaaaa taatcaacga caaattctcc ggtaaaagca
     1261 aaggcttcgg ttttgttgaa atgcctaacg atgacgaagc gcgtaacgcg atgaatgcat
     1321 tgaacgggca ggaacttaac agtcgcgcta tgac
//
LOCUS       DAORQA010000406         1352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_19261_length_1352_cov_5.107941, whole genome shotgun sequence.
ACCESSION   DAORQA010000406 DAORQA010000000
VERSION     DAORQA010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19261_length_1352_cov_5.107941"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>908
                     /locus_tag="PK736_10285"
     CDS             <1..>908
                     /locus_tag="PK736_10285"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRC33818.1"
                     /translation="GKSSLAFDTIYAEGQRRYMESFSAYARQFLGNMQRPDVDQINGL
                     SPVISIEQKTTNTNPRSTVGTITEIYDFMRLLFARAGEAYSYMTGEKMVKLNDEQINK
                     LIYEDFFDKRISVLSPMVKGRKGHYRELFEQVMNMGFLRVRIDGDMYEVKKGLQADRY
                     KVHDIEVVIDRMTVNHASQLRLNESLKLAIKHSKGSLMILDEDSGKVKHFSQHLMCPT
                     TGISYDEPQPNSFSFNSPYGACPKCNGLGIVPEIDIDSVIPDRKKTIKQGGIVPLGEY
                     KENWIFAQITAIGKKHGFTINSKIED"
     assembly_gap    909..937
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <938..1316
                     /locus_tag="PK736_10290"
     CDS             <938..1316
                     /locus_tag="PK736_10290"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRC33819.1"
                     /translation="EALQIILYGSDELIQIDRDYGGGAKHSYNMAFEGIISFLQNTFA
                     ENASDALRKWIDSFMLSNVCKECNGARLKKESLHFKIYDKNIAQLSQLDITDLQTWFA
                     EIHKHLSERQTAIATEILKEILK"
BASE COUNT          435 a          266 c          281 g          341 t
ORIGIN      
        1 gtggaaaatc gtcactggca ttcgatacta tttatgccga aggacagagg cgatacatgg
       61 aaagtttttc ggcatatgca cgtcagtttc taggcaatat gcaacggccc gatgttgatc
      121 aaataaatgg tttgagtcct gttatttcta tcgaacaaaa aactaccaat accaacccac
      181 gcagcaccgt aggaaccatt accgaaattt acgattttat gcgtttgcta tttgcccgcg
      241 caggtgaagc atactcgtac atgacgggcg aaaaaatggt gaagctcaac gatgagcaaa
      301 taaacaaact gatatacgaa gatttttttg ataagcgcat aagtgtattg tcgcctatgg
      361 taaagggccg caaaggtcac tatcgcgagc tatttgaaca agtgatgaac atgggttttt
      421 tgcgtgtccg tattgatggc gatatgtacg aagtaaaaaa aggcctgcaa gccgaccgct
      481 acaaagtgca tgatatagaa gttgtgatag accgtatgac agtaaaccat gcaagccaat
      541 tgcgattgaa cgaatcatta aaacttgcta taaaacacag caaaggttcg ctcatgatat
      601 tggacgagga cagtggtaag gtgaaacatt tttcgcaaca tcttatgtgc cccactaccg
      661 gtattagcta cgatgaacca caacccaatt cgttttcgtt taactctcct tatggtgctt
      721 gccctaagtg taatggacta ggcatagtgc ccgaaataga tatcgattcc gtaattcccg
      781 atcgcaaaaa aacaataaag cagggaggca tagttccact tggtgagtat aaagaaaatt
      841 ggatttttgc tcaaatcact gccataggaa aaaagcatgg cttcactatc aattcgaaaa
      901 ttgaagatnn nnnnnnnnnn nnnnnnnnnn nnnnnnncga agcgctacaa ataattttat
      961 atggcagcga tgagttgata caaatagatc gcgattatgg cggtggcgcc aaacattcgt
     1021 acaacatggc tttcgaaggc atcatcagtt ttttgcaaaa tacctttgcc gaaaatgctt
     1081 cggatgcttt acgcaaatgg atcgattcgt ttatgctcag caatgtgtgc aaagaatgta
     1141 acggagcacg attgaaaaaa gaatcgttgc actttaaaat ttacgacaaa aatattgcac
     1201 agctttccca actcgacatt accgacttgc aaacatggtt tgccgaaatt cacaaacacc
     1261 tgagcgaaag acaaacagca atcgccaccg aaattttgaa agaaattttg aagtgagaag
     1321 atacttaggc gttccatcgc caccaatacc tg
//
LOCUS       DAORQA010000407         1328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_19677_length_1328_cov_10.203456, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000407 DAORQA010000000
VERSION     DAORQA010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19677_length_1328_cov_10.203456"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..>1328)
                     /locus_tag="PK736_10295"
     CDS             complement(210..>1328)
                     /locus_tag="PK736_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRC33820.1"
                     /translation="ISIKIYNPGVNLGKNIFSKLNKFATDFRGANQRMHNKTFVVDGK
                     IVITGGRNIADEYFDYDHEYNFRDRDVLLLGKIASDVHNSFEQFWNSPLSVDICELAN
                     TESPQNPDSVTYNALHNYACNPENFWPQVREKIKNLPAAFDAIQKSGGLVWLDSVYFI
                     SDIPGKNDGTQGLEGGGVCTEALIQLIKNAKHTIAIQSPYLITNDASRKLLLDAVKRG
                     VKIKILTNSLASTDNLEAFSGYQRDRKKLLATGVRIFEFRPDAAERYKVMTGDLQSKL
                     NYTPVFGLHAKSMVIDGNITVIGTFNFDPRSANLNTECVAVIHSNKISSSVLNGMEIE
                     FNPENSWETTKQWNPDSDVGNIKRLKTWTKKIVPKNIL"
BASE COUNT          386 a          264 c          230 g          448 t
ORIGIN      
        1 ttacaatact atttgaaatt tgaattctat gaaataaata aactatgagt ttcctctgca
       61 aagtctaaaa aggataactg ccactaattc accaaaacac taaatcccac aaaatattat
      121 aaatgatttg tttacgactt agtgaaattt tgtgccttcg tgtttttgtg gcaaaagtat
      181 tttttcggag tcgactcatc tataaaagtt tataaaatat ttttcggcac aatctttttt
      241 gtccaggttt ttagccgttt tatgtttccc acatccgaat caggattcca ttgctttgtt
      301 gtttcccacg aattttcggg attgaattct atttccatac cattcaaaac actggaagaa
      361 attttattgg aatgtataac agccacacat tctgtattta aatttgcact acgagggtcg
      421 aagttgaaag tacctatgac agttatgttt ccatctatca ccatcgactt tgcatgtaag
      481 ccaaacacag gagtgtaatt taatttcgat tgcaaatcac ccgtcattac tttataacgc
      541 tccgctgcat cgggccggaa ttcaaaaatt cgcacacctg ttgcaagtaa tttttttcta
      601 tcacgttggt aaccactaaa tgcttcgaga ttatcggttg aagcaaggct gtttgttagg
      661 atttttattt tcactccccg cttaacagca tcgagcaaaa gtttacgact cgcatcgttc
      721 gtaattaaat aaggcgattg aattgcaatt gtatgtttgg cgttttttat aagttgaatc
      781 aatgcttcgg tgcatacacc gccaccttca agcccttgag tgccatcgtt tttgccggga
      841 atatccgata taaagtacac gctatcgagc caaaccaaac cgcccgattt ttgaatcgca
      901 tcaaatgcag caggaagatt ttttattttc tctcgtacct gcggccaaaa attttcggga
      961 ttgcatgcat agttgtgtaa tgcattgtaa gttaccgaat cagggttttg tggcgattct
     1021 gtatttgcta attcacatat atcaacactc aatggactgt tccaaaattg ctcgaatgaa
     1081 ttatgcacat cactggcaat tttaccgagt aacaaaacat ctctgtcacg aaaattatat
     1141 tcatgatcgt aatcgaaata ttcatcggca atattgcgcc ctccggtaat tactattttg
     1201 ccatcgacca caaaagtttt gttgtgcatg cgttggttag cgccacgaaa atcggtggca
     1261 aacttgttta acttgctaaa tatatttttg ccaaggttca ctccggggtt gtaaattttt
     1321 atcgaaat
//
LOCUS       DAORQA010000408         1321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_19800_length_1321_cov_3.590047, whole genome shotgun sequence.
ACCESSION   DAORQA010000408 DAORQA010000000
VERSION     DAORQA010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19800_length_1321_cov_3.590047"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PK736_10300"
     CDS             complement(<1..628)
                     /locus_tag="PK736_10300"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRC33821.1"
                     /translation="MANVIEQFETEEVKHFIEAHINDNAKELALKFRGKVSFDINIVS
                     SQIELWQHAKKKLPTWCAANALFTKLMYEQCTSEKVAQYKSTLFSGKKLLDATAGSGV
                     DAFFIGKNFKDVTMLETDYLKVKLLEYNFKKLKPPFKYNINNTDAESWLHSNKEYDLI
                     YFDPDRRDENKNRNILFELCSPDLMKIVDILNFSIASVAVKLSPLLDIQ"
     gene            764..1321
                     /gene="purQ"
                     /locus_tag="PK736_10305"
                     /pseudo
     CDS             764..1321
                     /gene="purQ"
                     /locus_tag="PK736_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930791.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
BASE COUNT          402 a          250 c          238 g          431 t
ORIGIN      
        1 attgtatgtc aagcaaaggc gataacttga cagcaacact ggctatagaa aaattcagta
       61 tatcaactat cttcatcaaa tcgggcgaac acaattcaaa aaggatattc cggtttttat
      121 tttcatcgcg cctgtcagga tcaaaataaa tgagatcgta ttccttgttg gagtgcaacc
      181 agctttcagc atcagtatta ttgatattat atttaaatgg tggtttgagc tttttaaaat
      241 tgtattctaa caatttcact tttaaataat cggtttcgag cattgttaca tctttgaaat
      301 ttttaccaat gaaaaatgca tcaactccac tacctgctgt tgcatctaaa agtttttttc
      361 cgctaaataa tgttgatttg tattgcgcca ctttttccga tgtgcactgc tcatacatca
      421 gttttgtaaa caatgcattt gctgcgcacc aggttggcaa tttctttttt gcatgttgcc
      481 atagttcaat ttgtgatgat acaatattga tatcgaaact cactttgcct cgaaacttta
      541 atgcaagttc ttttgcatta tcatttatat gggcctcaat aaaatgtttt acttcttccg
      601 tttcgaattg ctcaataaca ttcgccatta tatcacatat ttaactaacc aaaaagcaat
      661 gaaaaaactt cgtccatttt cgaaacagct actattttca tgttggtagc attttgaaga
      721 tcttcaattt ttttgtgata actggaaagc cagacataaa aacgaagttg ttaaactatg
      781 gcacaaagac catgacttac agggttgtga ttttattatt cttcccggtg ggttttcgta
      841 tggcgattat ttgcgttcgg gcgccattgc acgattttca ccaatcatgc agcaagtaag
      901 cgagtttgcc gacaagggtg gatttgtgct tggcgtttgc aatggatttc aaattttgtg
      961 tgaagccaac ttattacccg gcacactgct acataacaat accagaaaat ttatttgcaa
     1021 aaatgtatac atcaaagcgg taagcgatga attcttaatc acctcgaaac ttaacactca
     1081 caaagctttg aaaataccta ttgcacatgg tgaagggaag ttttttgccc ccgatgaagc
     1141 catgcgagaa atatatcaat acaaccagat tgtgtttaaa tactgcgatg agaaagctgt
     1201 tgtgaccttt gacgcaaacc ccaatggcag tttagattgc atagcaggaa taagcaatcg
     1261 cagaaaaaat gtattcggaa tgatgccgca tccggagcgt gctgccgata ctttactagg
     1321 c
//
LOCUS       DAORQA010000409         1319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_19812_length_1319_cov_10.029272, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000409 DAORQA010000000
VERSION     DAORQA010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19812_length_1319_cov_10.029272"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..500)
                     /locus_tag="PK736_10310"
     CDS             complement(144..500)
                     /locus_tag="PK736_10310"
                     /inference="COORDINATES: protein motif:HMM:NF027575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4907 domain-containing protein"
                     /protein_id="HRC33822.1"
                     /translation="MRYFIFWGLFFAACNNSSLKEQSPTSVSAPIGLRVKIETFKNAD
                     IKNNPVTTGYGYRIFMNDTLRVEQPNIPAVPGNNGFATEADAFITASLVKEKIELGIM
                     PPTITLTELDSMMVKY"
     gene            complement(504..>1319)
                     /locus_tag="PK736_10315"
     CDS             complement(504..>1319)
                     /locus_tag="PK736_10315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33823.1"
                     /translation="ARTGASAFSYIDEGFVVNGDGTNGKELWKYDPVNNAWSALKNFP
                     ANGVEQSSVFAENGKAWVCCGSDGSGGTSNLWQYNIGPNTWTQKSSFPGNARWNAAAF
                     MLDGKGTVGLGLSNGKLYNDVYQYDRFLNSWLQVDSFPGMARIGTTAFAEGDYGYICG
                     GRDNGQYFNDLWSFGPPIDTGLIDSVPEFCSIDLTPNATKGKLYLDCDNVINKKLEFN
                     LYDLKGRIVYSQSFNMQTKFLELNISKYREGIYIYVINCNDLFVTSGKLAIIQ"
BASE COUNT          389 a          283 c          246 g          401 t
ORIGIN      
        1 ttgcaaagta tcacaaggac tacccaaaaa aagcccccgg caacatgtcc aaccttacgc
       61 aaatgcatat tagcatagca actcttaacg attaaaagct ccgagtttta taaaaacgat
      121 ttcattattt tcgataattc gattcaatac ttcaccatca tactatctaa ctcggttaat
      181 gtaatggttg gtggcataat tccaagttca attttttcct taacaagcga tgctgttata
      241 aacgcatcgg cttcggtagc aaagccatta tttccgggta ctgctggtat gttgggttgt
      301 tcaactctta aagtgtcgtt cataaaaatg cgatagccat aaccagttgt aacaggattg
      361 tttttgatat ctgcattttt gaaagtttcg attttaactc gcaaaccaat tggggcggaa
      421 actgaagttg gtgattgctc ttttaaactg ctattgttgc atgcggcaaa aaatagtccc
      481 caaaaaataa agtagcgcat ggattattga ataatggcaa gttttccact tgtaacaaac
      541 aggtcgttgc agttgattac ataaatataa attccttcgc gatattttga aatattcagt
      601 tcaagaaatt ttgtttgcat attgaacgat tgtgagtaaa caatgcgccc tttcaaatcg
      661 taaagattga attcaagttt tttgtttatt acattatcac agtcgagata gagttttcct
      721 tttgtggcat ttggtgtaag gtcgatgctg caaaattcag gcaccgagtc gataagtccg
      781 gtatcgatgg gcggcccaaa agaccaaaga tcattgaagt attgtccatt atcgcgccca
      841 ccgcaaatgt atccataatc gccttcggca aatgcggtgg tgcctatacg tgccattccg
      901 ggaaaagaat ctacttgcaa ccagctattc aggaacctgt cgtattgata cacatcgtta
      961 tacaatttac cattgctcaa tcctagtcca acagttcctt tgccatcgag cataaatgct
     1021 gctgcattcc aacgtgcatt accgggaaac gaactttttt gtgtccatgt atttggacca
     1081 atattgtatt gccacaaatt ggatgtgcct ccgctgccat cgctaccaca gcaaacccat
     1141 gccttgccat tctctgcaaa cacggaagat tgctcaacac cattagcagg aaagtttttc
     1201 aaagctgacc atgcattatt aaccgggtcg tatttccaca attctttgcc attggtgcca
     1261 tcgccattta ctacaaaacc ttcgtcaata tatgaaaatg ccgaagcacc agttcgggc
//
LOCUS       DAORQA010000410         1298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_20165_length_1298_cov_7.870475, whole genome shotgun sequence.
ACCESSION   DAORQA010000410 DAORQA010000000
VERSION     DAORQA010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20165_length_1298_cov_7.870475"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PK736_10320"
     CDS             <1..1059
                     /locus_tag="PK736_10320"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRC33824.1"
                     /translation="NHYKQHDAVLKTIIKCSDITTKYTDNKGVQKPHTISYNDLKQMA
                     DVVFIALHGRPGEDGQVQQHLDALHIPYNGSGVASSQTTINKYETNEILLHHNLKAAR
                     HDLFYKNDWADDENKFIAAIEKKFSYPFIAKPVDDGCSSAVKVIRNSEVLSAFCKLLF
                     RKAESDWNSDAEKTLHLKPKEEFPVKQNILIEELIAANGCDHFLEITGGMLMKVNDKG
                     EKYFDVFEASEALSEGDILSLEEKFLAGQGQNITPARYSANAEHNAIISQKIKDQLGK
                     AAMLLKVEGYCRIDAFVRINKPTDVEVIFIEVNSLPGMTPATCIYHQAALDNLKPYDF
                     IDGILDFAMRKNKVGSLS"
BASE COUNT          429 a          281 c          257 g          331 t
ORIGIN      
        1 aaccactaca aacaacacga tgccgtactg aaaacaataa taaagtgcag cgacattaca
       61 acaaagtata ctgataataa aggcgtgcaa aaaccccata ccatttcata taacgatttg
      121 aagcaaatgg ccgatgtggt ttttatagca ctgcatggtc gcccgggcga agatggacaa
      181 gtgcaacaac atctcgatgc cttgcacatt ccatataatg gcagtggggt agcatcgtca
      241 caaaccacca tcaataaata tgagaccaac gagattcttt tgcaccataa ccttaaagcc
      301 gcacgtcacg atttgtttta taaaaatgat tgggccgatg atgaaaataa atttattgca
      361 gcaatagaga aaaaattctc ttatccattt attgccaagc ccgtggacga tggttgcagc
      421 tctgctgtaa aagtaatacg taatagtgaa gtgttgagcg cattttgtaa actgctgttt
      481 cgcaaagccg aaagcgattg gaatagcgat gccgaaaaaa cgctgcactt aaaacccaaa
      541 gaagaatttc ctgtaaagca aaatatattg attgaagaat taatagctgc aaatggctgt
      601 gatcattttt tggaaattac cggtggtatg ctcatgaaag taaatgataa aggcgaaaaa
      661 tatttcgatg tgtttgaagc gagcgaagca ttaagcgaag gcgatatact ttcgctggaa
      721 gaaaaatttc ttgccggtca gggccaaaac ataactcctg cacgatactc cgcaaatgcc
      781 gagcataacg ccatcatttc acaaaaaata aaagaccaac tcggcaaagc cgctatgctg
      841 ctaaaagtag aaggttactg ccgcattgat gcatttgtgc gcataaataa acctaccgat
      901 gtggaagtta tttttataga agtaaactca ctgcccggta tgacacccgc cacatgcata
      961 tatcaccaag ccgcactcga caacttaaag ccctacgatt ttatcgatgg cattttagat
     1021 ttcgcaatgc gcaaaaacaa ggtgggatcg ctatcataat attttagcat cgtatacagg
     1081 tagggttgcg ttgcacgcct ttggccatgg tttgtgtccc cacaaaccat catcacgatg
     1141 cctttattgt ccccaaggtt tgtgtcccca caaaccatca attctcttcc actctaaata
     1201 acacggatta aaatccgtag ctacaaaata ctccgttcct acggaactca tgctgtgtat
     1261 atctttgatg cgtaagcaat agagccgtag gcacgact
//
LOCUS       DAORQA010000411         1296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_20217_length_1296_cov_3.867849, whole genome shotgun sequence.
ACCESSION   DAORQA010000411 DAORQA010000000
VERSION     DAORQA010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20217_length_1296_cov_3.867849"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1296)
                     /locus_tag="PK736_10325"
     CDS             complement(<1..>1296)
                     /locus_tag="PK736_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRC33825.1"
                     /translation="IAPFDYLWSNGNTNQTANSLAPGSYSVTVTDSHGCTSQCSANIS
                     DGPDFSIAISGTPSQCNSCNGTTTTTLTGGVAPVTYLWNNGNTNSNLSALCSGTYSIV
                     ATDANGCTVSETIVIIDQSETISCNISKTNVTCNGAQNGTASVNVTQGTAPFTYLWSN
                     GKTTSGISGLGVGTFTVTVSNINGCSSSCQVIITQPVKVGCNITVAANALCGACTGSL
                     VADQQNTVGTVTFLWSNGATTKTITNLCAGTYTVTVSRTNGCSSSCSAVVGNTLIPIT
                     ISKTIVNNNACNNTCNGSVSVTATGGTSYTYLWSNGATTATAAQLCQGFYTVTVTDIS
                     GCTGTTSAEVKGKTTNVLATITNQTIPKCGTPCIGKLKVNGSGGTQYSYIWSTNPAKT
                     AQTISNICPGTYTVTVTDVSGCTATASIQLFPYVNTLNC"
BASE COUNT          360 a          263 c          266 g          407 t
ORIGIN      
        1 aacagtttaa agtattaaca taagggaata attgaataga cgctgttgct gtgcagcccg
       61 aaacatcagt aactgtaaca gtatacgttc ccgggcaaat gtttgaaatg gtttgcgctg
      121 tttttgcagg atttgtgctc caaatatatg agtattgtgt acctccgctt ccattaactt
      181 tcaattttcc tatacacggt gttccgcatt ttggtatagt ttgattagtg atagtagcca
      241 aaacatttgt cgtttttcct tttacttcgg ccgatgttgt tcctgtacaa ccactaatat
      301 ctgtaacagt aacagtatag aatccttgac acaattgcgc tgcagtagca gtagttgcgc
      361 cattactcca taaataagtg tatgatgttc cacccgttgc agttacactc acgcttccat
      421 tacaagtatt gttgcatgca ttgttattca ctatagtttt agaaattgta attggaataa
      481 gggtattacc aactacagcg gaacaagatg aggagcagcc attggtcctg ctcacggtaa
      541 ccgtatacgt tccggcacat agattagtta ttgtttttgt agtagcgcca ttgctccata
      601 aaaatgttac tgtaccaaca gtgttttgct gatcggcaac caacgaaccg gtgcacgctc
      661 cacatagtgc attggctgct acggtaatgt tgcatcctac ttttaccggc tgtgtaataa
      721 ttacctggca agatgatgaa catccgttta tattgctcac agtaacagta aacgttccga
      781 caccaaggcc ggatatacct gaagttgttt tgccgttgct ccaaaggtaa gtgaatggag
      841 cggtaccttg agtaacattt accgatgcag ttccgttttg tgccccatta catgtaacat
      901 tagtttttga aatgttgcac gaaattgttt cagattggtc aataattaca attgtttccg
      961 atacagtgca gccatttgca tcggttgcca ctatcgagta tgtgccggaa cacaaagctg
     1021 aaagatttga atttgtattg ccattattcc ataaataagt tacaggagca acaccgcctg
     1081 ttaaagttgt agtggtggtt ccgttacaac tgttgcattg cgatggtgta ccacttatag
     1141 caatagaaaa atcggggcca tcacttatgt tggcagaaca ttgcgaagta caaccatgcg
     1201 aatctgtaac ggttacgcta tatgaacccg gtgccaaact atttgccgtt tgattcgtat
     1261 ttccattact ccataaataa tcaaatggtg caatgc
//
LOCUS       DAORQA010000412         1282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_20464_length_1282_cov_2.967400, whole genome shotgun sequence.
ACCESSION   DAORQA010000412 DAORQA010000000
VERSION     DAORQA010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20464_length_1282_cov_2.967400"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PK736_10330"
     CDS             complement(<1..772)
                     /locus_tag="PK736_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HRC33826.1"
                     /translation="MIPFSPPRIDQKIIDEVVQVLKSGWITTGPRTKMFEKKLAQYTG
                     VSNVLCLNSATAGLEIMLRWLGVGPGDEVILPAYTYSATANVVMHTGATPVLVDVNDD
                     DFCINLHRIRSAITERTKVIMPVDFGGWPCDYKALHALVNEKEVKLKFIPANDIQHKL
                     NRIMILADAAHSLGAITDGVKSASIPDAAVYSFHAVKNLTTAEGGAVVLNLPLPFDKA
                     AIYNFLCVYTLHGQNKDALAKMQKGNWRYDIVEAGYKCN"
     gene            complement(1027..>1282)
                     /locus_tag="PK736_10335"
     CDS             complement(1027..>1282)
                     /locus_tag="PK736_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33827.1"
                     /translation="TDMLDSNVVKQSEAPTLEQMQESKLLASNPSNLELRFSLWSSAL
                     NLIAKKPLLGYGIGDINSQLVTYYKELTINTCKYFWQQAS"
BASE COUNT          383 a          274 c          232 g          393 t
ORIGIN      
        1 tgttgcattt ataaccggct tctacaatgt cgtaccgcca atttcccttt tgcatttttg
       61 ccagggcatc tttgttttgt ccatgcaacg tatacacaca taaaaaatta taaatcgctg
      121 ctttatcgaa tggcagaggc aggtttaaaa ccactgcacc gccctcggcc gttgtaagat
      181 ttttaacagc atgaaacgag tatacagccg catcgggaat cgatgcactt tttactccat
      241 cggtaatagc gcctagcgaa tgtgcggcat cggccaaaat cattatgcga ttaagtttat
      301 gttgaatatc gttggcggga ataaatttta gttttacttc cttttcgttt accaatgcat
      361 gcagcgcttt gtaatcgcaa ggccacccac caaaatccac aggcattatc actttggtac
      421 gctcggtaat agcactacgt atacggtgta ggttgataca aaagtcatca tcatttacat
      481 caaccaaaac aggtgtagca ccagtgtgca tcaccacatt ggcagtagcg ctataggtat
      541 aggcgggcaa aataacttca tcgccagggc ctactcccag ccaccgcaac ataatttcga
      601 gaccggcagt agcactattt aaacaaagca catttgatac tccagtgtac tgcgccagtt
      661 tcttttcaaa catttttgtg cgcgggccgg ttgttatcca tcccgatttt aaaacttgta
      721 ccacttcatc aataattttt tggtcgatgc gtggtggcga aaatggtatc atatgatttc
      781 agtgttttat atttacaaat gtatttagta tcagctattt tgaaaatagt tttatatcgg
      841 ttctgtttct gtgagttatc aatattgtta cgagcataaa aaatattatt cctacagaat
      901 gttgcataat cgattctacc attgcattgg ctgccaaaat gaacaaacaa agaaacccca
      961 aaatatctct ttgttgcatg gccataaaaa aacacaacaa taagtaagca ccaaaaacaa
     1021 caaagcctat gatgcctgtt gccaaaaata tttgcaagta ttgattgtga gctctttgta
     1081 atatgtcaca agctgcgaat tgatatcgcc aataccataa cccaacaaag gtttctttgc
     1141 aattaagttt aaagcactgc tccataatga aaaccgcaat tctaaattac ttggattgga
     1201 cgcaagtaat ttactctcct gcatttgttc gagcgttggc gcttctgatt gttttacaac
     1261 atttgaatca agcatatccg tt
//
LOCUS       DAORQA010000413         1273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_20629_length_1273_cov_3.738095, whole genome shotgun sequence.
ACCESSION   DAORQA010000413 DAORQA010000000
VERSION     DAORQA010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20629_length_1273_cov_3.738095"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PK736_10340"
     CDS             <1..785
                     /locus_tag="PK736_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33828.1"
                     /translation="AKKEGDVFKNPFYYFWNGEGTKLKNTSVCIAPQTLKVMGKPDLG
                     SGMYLSEGDFKGVEKKNFYCPNCGYDTSLYLNAPFRQMSQAVETSTGFENIPVVMDVV
                     GDNYTMMDYNWNRSFYTQEEMVKIYIESAHEPCKTVVSDSTDHKIVIRNPACEYGKWR
                     KERVGIIGRHGMTYGKYRFKCKLTELLSKDGVWNGMTNAIWMIFPAGATEWNDRRICN
                     KEGYMATYWGGYDDKKIPYSGYSEIDFENRTLLPRLRFSTYV"
     gene            811..>1273
                     /locus_tag="PK736_10345"
     CDS             811..>1273
                     /locus_tag="PK736_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33829.1"
                     /translation="MNDWNVGFPDDITKHRGDITVACTNWDMACPEPENYGDGCKSIT
                     YNGQEFLSHRWQKNYRALTQKSYQSDDELFGSPFYYFEIAWQPTQIIWRIGPSPDKMR
                     IVGYMNDKVTMIANNQMLPIITQEWHNTKWWPGTPFDQGNIPFPAKDLVGEF"
BASE COUNT          411 a          240 c          285 g          337 t
ORIGIN      
        1 ttgcgaaaaa agaaggcgat gtatttaaaa acccttttta ctatttttgg aatggcgaag
       61 gcacaaaact caaaaacacc tcggtgtgca ttgcccctca aactttgaaa gtaatgggta
      121 agcccgattt aggatcgggc atgtatttga gtgaaggtga ttttaaagga gtagaaaaga
      181 aaaactttta ttgccccaat tgcggctacg atacttcctt atatttaaat gcacctttca
      241 ggcaaatgtc gcaagcggta gaaacatcaa caggtttcga aaatattccg gtagtgatgg
      301 atgtggtagg cgataattat actatgatgg attataactg gaatcgcagt ttttacactc
      361 aggaagaaat ggtaaaaatt tacatcgagt cggcacacga gccatgcaaa actgttgtaa
      421 gtgattcgac agatcacaag attgttattc gtaatccggc atgtgaatat ggcaagtggc
      481 gcaaagaacg tgttggtata attggccggc atggaatgac atatggtaag taccgtttca
      541 aatgtaagct taccgaactg ctgagtaaag atggagtatg gaatggaatg accaatgcaa
      601 tatggatgat ttttcctgcc ggtgctaccg agtggaacga ccgtagaata tgtaataaag
      661 aaggatacat ggctacatac tggggcgggt acgatgataa aaaaattcct tactctggtt
      721 acagcgaaat agatttcgaa aaccgtacct tattgccccg attgcgattt tccacctacg
      781 tataaaaata acgtatacga tatacgtagt atgaacgatt ggaatgttgg cttccccgat
      841 gatataacca aacaccgtgg tgatataact gttgcctgta ccaattggga tatggcatgc
      901 cccgagcccg aaaactatgg cgatggatgc aaatccataa catacaatgg tcaggaattt
      961 ttatcgcacc ggtggcaaaa aaattatcgc gcgcttacgc aaaaaagcta tcaaagcgat
     1021 gacgaacttt ttggctcacc attttattat ttcgaaatcg cctggcagcc aacacaaata
     1081 atttggcgca ttggcccttc gcccgataaa atgcgcatag tgggttacat gaatgataag
     1141 gttaccatga ttgccaacaa ccaaatgttg ccaattatta cgcaagagtg gcacaataca
     1201 aaatggtggc ccggcacgcc attcgatcaa ggcaatattc catttcctgc aaaagattta
     1261 gtaggcgaat ttt
//
LOCUS       DAORQA010000414         1271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_20660_length_1271_cov_6.206414, whole genome shotgun sequence.
ACCESSION   DAORQA010000414 DAORQA010000000
VERSION     DAORQA010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20660_length_1271_cov_6.206414"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /gene="gap"
                     /locus_tag="PK736_10350"
     CDS             <1..939
                     /gene="gap"
                     /locus_tag="PK736_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRC33830.1"
                     /translation="VFKQLIADDTIQVMAINDLGEGATLAHLLKYDSIHGIFQADIKY
                     ENNHLIVNGKHVELLHEKNPADLPWQKMGIDVVIESTGKFAKEEGANLHLQAGAKKVI
                     ISAPASGNIKSVVLGVNENILDGTEKIISNASCTTNCASPMMKVLQDNWDVEAAYITT
                     VHAMTGDQNLHDAPHKDLRRARCAPASIIPTTTGAAKAINDIFPHFKGQVGGAGIRVP
                     VVDGSLTDITCILKQSTDVASINAKFKEAANGPMKNILQYCEDPIVSIDIVGNKYSCI
                     YDSLLTSVLGKMVKVVGWYDNEMGYSTRLYELVKIV"
BASE COUNT          408 a          237 c          266 g          360 t
ORIGIN      
        1 gttttcaagc aactaattgc agacgacaca atacaagtaa tggccatcaa tgatttgggc
       61 gagggcgcta cgctagcgca tttactaaaa tacgattcta ttcatggcat ttttcaggcc
      121 gatattaaat atgaaaataa tcatttgata gtaaatggaa aacatgttga attgcttcac
      181 gaaaaaaatc ctgctgattt gccttggcaa aaaatgggaa tcgatgttgt gatagaatcc
      241 accggaaaat tcgcaaaaga ggaaggtgcc aatttgcatt tacaggccgg agcaaaaaaa
      301 gtaatcatct ccgcacctgc aagtggcaat ataaaatcgg tagtgttggg tgtaaacgaa
      361 aatattttgg acggtaccga aaaaataatt tctaatgcat cgtgcacaac caactgtgca
      421 agccctatga tgaaagtgtt gcaagataat tgggatgtgg aggctgcata tattacaaca
      481 gtgcatgcaa tgacaggcga tcagaatttg cacgatgcac cgcataaaga tttgcgcaga
      541 gctcgttgtg cacctgcatc tattattcct accaccactg gtgcggcaaa ggccatcaac
      601 gatatttttc ctcacttcaa agggcaagta ggtggagccg gtattcgtgt gccggttgtt
      661 gatggctcgt taaccgatat tacatgtatt ttaaaacaaa gcaccgatgt ggcaagcatc
      721 aacgccaagt ttaaagaagc ggccaacggc cctatgaaaa atattttgca atactgcgaa
      781 gaccctattg tgagtatcga tatagttggc aataagtact catgtattta cgattcgttg
      841 cttacaagcg tgctaggcaa aatggtaaaa gttgttggat ggtacgataa tgaaatgggt
      901 tactccacac gcttgtatga attggtgaag attgtgtagt ttttcatttt agttctcata
      961 gatattagat atttttttcg gaatttgttt aagggaaaat tgctcatggg taacttatat
     1021 tctcaaaaaa tattttcgga atctatacct tggaaaaata ctttttaaaa agaccacaga
     1081 ttattttcga acatgaagcc caacaaaaaa ccccgagtaa acacccgggg ctttgtatga
     1141 ataatatttt attatttcga aacggagaat taatatcctc ccattccgtc cattccaccc
     1201 ggcattgaag gcgctggtga agcagcttct ttaatgtcgc tcaatacaca ctcggtagtc
     1261 aataacatac c
//
LOCUS       DAORQA010000415         1260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_20894_length_1260_cov_9.876349, whole genome shotgun sequence.
ACCESSION   DAORQA010000415 DAORQA010000000
VERSION     DAORQA010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20894_length_1260_cov_9.876349"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..914
                     /locus_tag="PK736_10355"
     CDS             216..914
                     /locus_tag="PK736_10355"
                     /inference="COORDINATES: protein motif:HMM:NF017966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromophore lyase CpcT/CpeT"
                     /protein_id="HRC33831.1"
                     /translation="MKLISKLSCPIKLLFCCAILNTATIANAIAQEKAAPVKTADGAT
                     LVDNTDLYNLFEMMSGKFSSEAQSKADTSYFNISLKMHPIWQSRTDGHWFYVEQAMAT
                     KLDKPYRQRVYHLYLYNDKKIASSVFEIPNPAAYVGSCEKDNPLSNLTPDSLISREGC
                     TIFLEKMNTEVFSGSTPQKQCKSSLRGAAYATSEVTITSGLIISWDRGWDDKDKQVWG
                     AEKGGYEFDKLEDY"
BASE COUNT          389 a          233 c          235 g          403 t
ORIGIN      
        1 atctttgaat accacatttc tataattctt tggatggatg tccggtttca tcttatttat
       61 tttattttag ggtcgcaaaa gtaaaccttt tttctctttt tcaaaatgtt ttgtgctttt
      121 tagttttatt tgtaccaaaa tgcgcctgac ttaacttccg catttagggc tcatcgaaat
      181 aaaattgaat taaaatcttt aaaatataaa ttgaaatgaa attgatttcg aaattaagtt
      241 gccctattaa attgcttttt tgctgtgcga ttttaaatac tgcaaccatt gcaaatgcca
      301 tagcacagga aaaagccgcc ccggtaaaga ctgccgatgg tgctactttg gttgacaata
      361 ccgatttgta caatttgttt gaaatgatgt cgggaaaatt ttcgagcgag gcacaatcca
      421 aagccgatac ttcatatttt aatatcagcc taaagatgca tccaatatgg caaagccgaa
      481 cagatgggca ttggttttat gtggaacagg ccatggccac aaaacttgat aaaccttacc
      541 gtcagcgggt gtatcattta tatttgtaca acgataaaaa aattgccagc agcgttttcg
      601 aaattcccaa tcctgccgca tacgttggca gttgcgaaaa ggataatcca ttatcgaacc
      661 ttacacccga ctcgttaatt agccgcgaag gctgcaccat ttttttagaa aagatgaaca
      721 ccgaagtatt ttcgggcagc acgccacaaa agcaatgtaa aagttccttg cgtggtgctg
      781 cttatgccac atcggaagtt acaattacat caggcctcat catatcatgg gaccgcggtt
      841 gggacgacaa agacaaacaa gtgtggggtg ccgaaaaagg cggatatgaa tttgataaat
      901 tggaggatta ttaggctttt gtggaaggtg aaataaaaac cccaacaagg tgaaaatgat
      961 ttattttatt ttcaaattcg aattttattt gctatccgca atgttcttgt ttctttttct
     1021 taatgagtat tactttgaaa cagaaagtat ctgcatatta aaaatgttat cgcattcttt
     1081 atcatgtaaa ccatcgtact ggcaccaata aatgttctca ttaaaattga ccaatacatt
     1141 gttggttaaa gtacttttat caagataata aaaagtacta tcactgcttt gcgccacaca
     1201 ttgggcatgt attttggctc agtgtattca cgctcataga ttggtgctcc accgccaagt
//
LOCUS       DAORQA010000416         1257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_20960_length_1257_cov_7.200499, whole genome shotgun sequence.
ACCESSION   DAORQA010000416 DAORQA010000000
VERSION     DAORQA010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20960_length_1257_cov_7.200499"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /gene="thrC"
                     /locus_tag="PK736_10360"
     CDS             complement(<1..1080)
                     /gene="thrC"
                     /locus_tag="PK736_10360"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRC33832.1"
                     /translation="MKFYSTRDKEKLFSLKEALMLGMAPDGGLFMPEQIPHINIEHLL
                     KGNATIHECAYAVLSPFLLEDFDAQQISEMISAAFNFEVPIISLEKNLFLCELFHGPT
                     LAFKDFGARMMAQLMSAFLKNDNDEITILVATSGDTGSAVASGFFNVPNIKVVILYPA
                     GKVSTLQELQLTTYGGNITALKVEGTFDDCQALVKQAFADDELRNRARISSANSINIG
                     RLLPQMIYYAYTSIKMKKDTGSDCIMCVPSGNFGNLTAALMVKQMGFPIKHFYAATNA
                     NDTVPDYFKTKNYTPKKSQPTISNAMDVGNPSNFERMLCLYDNNFEKISTDISAIAIS
                     DDSTISMMKEVFNERKYLTDPHTAVA"
     gene            complement(1077..>1257)
                     /locus_tag="PK736_10365"
     CDS             complement(1077..>1257)
                     /locus_tag="PK736_10365"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRC33833.1"
                     /translation="TGAVGYNISGSGPAVFAVSSERETSEKIAHVWKNYFSEINSPCL
                     CITSSINKEGVKIIS"
BASE COUNT          380 a          253 c          210 g          414 t
ORIGIN      
        1 tgctacagca gtatggggat cggtcaggta ttttctttca ttaaatactt ccttcatcat
       61 gcttattgta gagtcgtcag aaattgcaat tgcagaaatg tcagttgaaa ttttttcaaa
      121 attattatca tacaaacata acatacgctc aaaattactc ggattgccaa catccatagc
      181 attggaaatt gtgggttgcg atttttttgg tgtgtaattt tttgttttaa aataatcagg
      241 tacagtatcg tttgcgttgg tagcagcata aaaatgttta attggaaatc ccatttgctt
      301 aaccataagc gcagcagtaa ggttgccaaa atttcccgaa ggcacacaca taatacaatc
      361 actgccggta tcctttttca tttttatgct tgtgtatgca tagtaaatca tttgtggtaa
      421 aagccttcct atatttatag aatttgccga agaaatgcgc gcacgattgc gcaattcatc
      481 atcagcaaat gcctgcttca ccaatgcctg acaatcgtca aatgttcctt ccacttttag
      541 tgctgtaata tttcctccat aggttgtaag ttgcaattcc tgcaaggtgc ttactttgcc
      601 agcaggatat aatattacaa ctttaatatt gggcacatta aaaaaaccgc tggccaccgc
      661 actacctgta tcgcccgatg tagcaacaag aatggttatt tcatcgttat cattttttaa
      721 aaatgcactc atcagctgcg ccatcatgcg agcaccaaaa tctttgaacg caagcgtagg
      781 accatgaaac aattcacata aaaataaatt tttctccaaa ctaataatgg gtacttcgaa
      841 attgaaagct gcacttatca tttcgcttat ttgttgtgca tcgaaatctt ctaacaagaa
      901 tggagataaa accgcataag cacattcatg tatggttgca ttgcctttca ataaatgctc
      961 aatattaata tgcggtattt gctcaggcat aaacaaaccc ccatcgggtg ccataccaag
     1021 catcaatgct tctttgagtg aaaataattt ttccttatct cgtgtggaat aaaatttcat
     1081 gatattattt ttacgccttc tttatttatt gatgatgtaa tgcatagaca agggctgttg
     1141 atttcactaa aataattttt ccatacatgt gcaatttttt cagatgtttc tctttcgctc
     1201 gacactgcaa aaactgccgg ccccgaaccg gaaatattat atcccactgc acctgtt
//
LOCUS       DAORQA010000417         1254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_21008_length_1254_cov_6.345288, whole genome shotgun sequence.
ACCESSION   DAORQA010000417 DAORQA010000000
VERSION     DAORQA010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21008_length_1254_cov_6.345288"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1254)
                     /locus_tag="PK736_10370"
     CDS             complement(<1..>1254)
                     /locus_tag="PK736_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRC33834.1"
                     /translation="GIFSTIGNASASGGPGVIFLFIFTSIACGFAAFAYAEFASLVPV
                     SGSAYTYSYVAFGELIAWIIGWALIMEYGIGNITVAISWSDYFTGLMQNFGIHIPDWA
                     TMDFLTAHNGYNDAVALMAKGTSFANLDAAVQSSYTAYVNAPQLGPLHIVADLPALAI
                     IILITALVYRGMRESRNASNIMVAIKLSVVLLVIAVGIFYIDTDNWTPFMPNGIPGVL
                     KGVSAVFFAYIGFDAISTTAEECKNPQRDLPKGMMWAIIICTILYVAIALVLTGMVNY
                     STLGVGDPLAFVFDSLNLKWMSFIIGISAVVAMASVLLVFQLGQPRIWMSMSRDGLLP
                     KKFATIHPRYKTPSFATIVTGFVVAVPALFMNLTMVTDLCSIGTLFAFVLVCAGVLRL
                     QMLPNPPERKFKTPYFNSRYVMPVLL"
BASE COUNT          421 a          283 c          237 g          313 t
ORIGIN      
        1 taataatacc ggcattacat aacggctatt aaagtatggc gttttaaatt ttcgctcggg
       61 tggattgggc aacatttgca aacgcaaaac accggcacat accaatacaa atgcaaacaa
      121 tgttcctata ctgcacaaat cggtaaccat agtaaggttc ataaataagg caggaacagc
      181 aaccacaaag cctgtaacaa tggttgcaaa cgatggcgtt ttatatcgag gatgaatggt
      241 cgcaaatttc tttggtaata atccatcgcg gctcatactc atccatatac gaggttggcc
      301 caattgaaag acgagtaata cacttgccat ggccactacg gcacttatgc caataatgaa
      361 cgacatccat tttaaattta atgaatcgaa aacaaaagca agtggatcac ctactccgag
      421 tgtagagtaa tttaccatgc ctgttaacac caatgcaatg gcaacatata aaatggtaca
      481 aataattata gcccacatca ttcctttcgg caaatcgcgt tgggggtttt tacattcttc
      541 ggctgtagta cttatagcat cgaaaccaat gtaagcaaaa aatactgccg atacaccttt
      601 caatactccg ggaattccat ttggcataaa tggcgtccag ttgtcggtat cgatataaaa
      661 tattcctaca gcaattacca ataagacaac actcaatttt atggccacca taatattact
      721 ggcattgcgg ctttcgcgca tcccgcgata caccagggca gtaatgagaa tgataatggc
      781 taatgcaggc aaatcggcaa ctatatgcaa aggccctaat tgcggtgcat taacgtaagc
      841 tgtatacgat gactgcacag cggcatcgag attagcaaaa cttgtccctt tggccataag
      901 tgcaacagca tcgttgtaac cattgtgggc tgtaagaaaa tccatggtag cccaatcggg
      961 aatatgaatg ccaaaatttt gcatcagccc ggtaaaataa tcgctccacg aaatggctac
     1021 agtaatgttt ccaatgccat attccattat caatgcccaa ccaataatcc atgctataag
     1081 ctcaccaaat gcaacataac tatatgtgta tgcgctgccg cttaccggaa caagcgaagc
     1141 aaattcagcg taagcaaaag ctgcaaagcc acatgcaata gatgtaaaaa taaatagaaa
     1201 tattaccccg ggcccaccac ttgcacttgc attgccaatg gtactgaata tgcc
//
LOCUS       DAORQA010000418         1242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_21230_length_1242_cov_8.303286, whole genome shotgun sequence.
ACCESSION   DAORQA010000418 DAORQA010000000
VERSION     DAORQA010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21230_length_1242_cov_8.303286"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1242
                     /locus_tag="PK736_10375"
     CDS             <1..>1242
                     /locus_tag="PK736_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC33835.1"
                     /translation="YNANHLFGFFSLFNGDALKSVELTKGGFPARYGSRLSSVIDMTM
                     KDGAKDKLHGEGGIGLISSRLTLEGPIKKGKSSFLVSGRRTYIDLLIAPLVAASTNGE
                     STAGYFFYDFNAKANYDFNDNNKLFVSGYFGKDKAYGRENYGGDKNKFSIGWGNATGT
                     ARWNHLFNEKLFANTSLIFSNYRFLIKNEETFDGDKYTLRYYTGIQDISAKFDLDYRP
                     GEKHIIKMGGQFTRHLFTPSALVTKGDGNDDVNNVSKISAIENAIYIEDDVRILPQLK
                     MNAGLRLAHFYSNGKNYFNPEPRLALAYNINESTSLKASFAIMNQYLHQLSNSGAGLP
                     TDLWVPATKNVTPQNSQQVAAGIARDYKDRNFALTIEGYYKTMNKVLQYREGASFIDI
                     GDPNQVDNDYNWEDKVTSGKG"
BASE COUNT          407 a          230 c          273 g          332 t
ORIGIN      
        1 gtataatgcc aatcacctgt ttggtttttt ctccttgttc aatggcgatg cactcaaaag
       61 cgtggaactt accaagggag gatttcctgc aagatatggt tcgcggctat catcggttat
      121 tgatatgacc atgaaagatg gtgccaaaga taaattgcat ggtgaaggcg gtattggttt
      181 aatatcatcg cgccttactc ttgaaggacc aataaaaaaa ggtaagagta gttttctggt
      241 aagtggcaga cgcacatata tcgatttgct catagcacca ttagttgcag cgtccacaaa
      301 tggcgaaagt acagcgggat attttttcta tgattttaat gccaaagcaa attacgattt
      361 caacgataac aataaattat ttgtaagcgg atattttgga aaagataaag cctatggccg
      421 cgaaaattac ggtggcgata aaaataaatt ttccatcgga tggggcaacg ctaccggtac
      481 agcacggtgg aaccatttat tcaacgaaaa attatttgcc aacacatcat tgatttttag
      541 caactaccgt ttcctgataa agaacgaaga aacatttgat ggtgataaat acacattgcg
      601 gtattacaca ggtatacagg atatttcggc aaagttcgat ttggattacc gcccgggcga
      661 aaaacatatc ataaagatgg gcgggcaatt cacccgtcac ttatttacac ccagtgcatt
      721 ggttacaaaa ggagatggca acgatgatgt aaataatgta agtaaaatat cggccataga
      781 aaatgcaatc tatatagaag atgatgtacg tatactgcca cagttaaaaa tgaatgccgg
      841 tttgcggttg gcgcattttt attctaatgg aaaaaactat ttcaatcccg aacctcgact
      901 ggcgctggct tacaacatca atgaaagcac atcgttaaaa gcatcgtttg caatcatgaa
      961 tcagtatttg catcagctaa gtaactcggg tgcaggtttg ccaaccgact tgtgggtgcc
     1021 ggcaacaaaa aatgttactc cccaaaattc gcaacaggta gcagcaggca tagcacgtga
     1081 ttataaggat cgcaattttg cactcaccat cgaggggtat tacaaaacaa tgaacaaagt
     1141 attgcagtac cgtgagggtg caagttttat tgatataggc gaccccaatc aggttgacaa
     1201 cgattataac tgggaagata aagttacatc gggtaaaggc tg
//
LOCUS       DAORQA010000419         1236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_21360_length_1236_cov_4.046571, whole genome shotgun sequence.
ACCESSION   DAORQA010000419 DAORQA010000000
VERSION     DAORQA010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21360_length_1236_cov_4.046571"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1236)
                     /locus_tag="PK736_10380"
     CDS             complement(<1..>1236)
                     /locus_tag="PK736_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HRC33836.1"
                     /translation="HFYFVSKRTIFFSIVCTLICFAAFAKQKSTIASDIIPLNATWKF
                     TRADSIAWMPATVPGSVFSDLLANKKIPNPYVENNETKVQWVQNFDWIYECSFDIDSG
                     VLQFNHVDINFEGLDTYADVFVNGNKILFADNMFRAWEQNIKSNLKTGKNTLRVYFYS
                     PVNWVFKNRNTSFVFPADNDNHPLKTSAHTRKSPMHYGWDVAPRLVGCGIYRNIKLHV
                     WNDMYINNVFVRTTSIIKNVALLDVQVNLLSANPDSVQIEIYDDAKSFKPIIRNYVKV
                     KGHHIFSIPVQIKNPDLWFPNETGAQPLYTFHIILRTKRDVQQTIVTTGIRTMQVVNE
                     IDKDGRNFYVMCNGNKIFARGANWIPPDAMSNVVTDSVYEAYFKIMHQLHFNMIRVWG
                     GGIYESNRFYDLADRYGIMV"
BASE COUNT          371 a          247 c          237 g          381 t
ORIGIN      
        1 caccataatt ccatatctat cggcaaggtc gtaaaagcga ttgctttcgt atatgccacc
       61 gccccacacg cgtatcatgt tgaagtgtag ttggtgcatt attttaaagt aagcctcgta
      121 tacactatcc gttacaacat tgctcatggc atcaggcgga atccagttgg caccacgggc
      181 aaatatttta ttgccattgc acatcacata aaaattgcgg ccgtctttat ctatttcgtt
      241 taccacttgc atggtacgaa tacctgtagt aacaatagtt tgctgcacat cgcgctttgt
      301 tcgcagaatg atgtgaaatg tgtaaagtgg ttgtgcccct gtttcgttgg ggaaccacaa
      361 atcggggttt tttatttgta ccggaatact aaaaatatga tgacccttta ctttcacgta
      421 attgcgaatg atgggtttaa aagattttgc atcatcgtat atttcaatct gcaccgaatc
      481 gggatttgca gaaagtaaat ttacctgcac atcgagcaag gctacatttt ttataatgga
      541 ggtggtgcgt acaaaaacat tattaatgta catatcgttc catacatgca atttaatatt
      601 tcggtaaatg ccacatccta ccaggcgtgg tgccacatcc caaccataat gcattggcga
      661 cttacgtgta tgtgcactgg tttttaacgg atgattatcg ttatcggcag gaaaaacaaa
      721 tgaggtgtta cgatttttga aaacccaatt caccggagaa taaaaatata cacgtaaggt
      781 attttttccc gtcttcaaat tcgattttat attttgctcc catgcacgaa acatattatc
      841 ggcaaacaaa attttgttgc cattaacaaa cacatcggca taagtgtcga ggccttcgaa
      901 atttatatcc acatgattga attgcaaaac tcccgagtcg atatcaaaac tacattcgta
      961 tatccaatcg aaattttgca cccattgcac ttttgtttca ttattttcaa cgtatgggtt
     1021 aggtattttt ttgttggcaa gtaaatcgct gaacacactg ccgggaacag ttgcgggcat
     1081 ccatgcaata ctatcggcac gggtaaattt ccatgttgca tttagcggaa taatatcgga
     1141 agcaatggta gatttttgtt ttgcaaaagc tgcgaaacaa atcagcgtgc aaacaatgct
     1201 aaaaaatatt gtgcgcttcg acacaaaata aaaatg
//
LOCUS       DAORQA010000420         1226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_21526_length_1226_cov_9.238258, whole genome shotgun sequence.
ACCESSION   DAORQA010000420 DAORQA010000000
VERSION     DAORQA010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21526_length_1226_cov_9.238258"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1226
                     /locus_tag="PK736_10385"
     CDS             <1..>1226
                     /locus_tag="PK736_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33837.1"
                     /translation="MNGGLGQVVMKNQLVQAGSFAEKIHALYTPGGDVWVIAQLNNSN
                     TYVTVLVTNAGVGAPSSQSIGTVHPFFVSGLRFSHDGSKLASCNYQASQIELFDFDYT
                     TGLLSNPATHTFPGNFPAYSIEYSPSGQYMYVGNVDVSPGLIYQFDLNAGNNTAILAT
                     AQLVLTVPNNYTGTIQLATDGKIYCPSWSSSFVSVINDPDMGGVACNGVANALSVTGQ
                     SYLGLPQWPAGTFGVVIKYDKLCLGDSTEFNIQNAAQTLITDWNFDDPSSGVNNTSSL
                     TNPKHLFTAVGTYNVRLIRVFTNLQSDTTYQPITIDNVVNINLGNDTTICIGQSVVLN
                     AGTSYTNYQWQNGNTNTTYTATGTGTYYVTAENGQCKGTDSIDVTAIQCSGPLAALSS
                     SDTLWCDKTCIDFFDL"
BASE COUNT          353 a          245 c          254 g          374 t
ORIGIN      
        1 tatgaatgga ggattggggc aagtggtaat gaaaaatcaa ttggtgcaag caggttcgtt
       61 tgccgaaaaa atacatgccc tgtatactcc cggtggcgat gtgtgggtga ttgcccaact
      121 caacaattca aatacatatg ttaccgtact tgttactaat gcgggtgttg gtgccccatc
      181 atctcaatct attggtactg ttcatccttt ttttgtaagt ggattacgct tttcgcacga
      241 tgggtcaaag ttggcttcgt gcaattatca ggcaagccaa attgagttgt tcgatttcga
      301 ttatacaaca ggtttacttt ctaatcctgc tacccatact tttccgggaa attttcctgc
      361 atatagcatc gagtattctc catcgggcca atatatgtac gttggcaatg ttgatgtttc
      421 gccaggattg atttatcagt ttgatttgaa tgcggggaat aatacagcga ttttagccac
      481 cgcacaattg gtgctaactg tgcctaataa ttatacaggc acaattcaac ttgccaccga
      541 tggaaaaatt tactgcccct catggtcgtc aagttttgtt tcagttatta atgaccccga
      601 tatgggtggt gtagcttgca atggtgtagc aaatgctttg tctgttacag gccaatctta
      661 tttgggatta cctcaatggc cggccggtac ttttggcgta gtaataaaat acgacaaact
      721 ttgtttgggt gattctacag aatttaacat tcaaaatgct gcacaaacgt tgatcaccga
      781 ttggaatttt gatgaccctt cgagtggagt caataacact tcatcgctta ccaatcctaa
      841 acatttattt actgcagtgg gaacctacaa tgttcgattg attcgtgtat ttacaaactt
      901 acaaagcgac accacctatc agccaattac tattgataat gttgtaaata taaatcttgg
      961 caacgatacc actatttgca taggacaatc ggtggtgctt aatgccggta caagttatac
     1021 aaattaccaa tggcaaaatg gaaacaccaa tacaacgtat acagcaacag gtacaggtac
     1081 atattatgta actgccgaaa acggacaatg caaaggaaca gattcgatag atgtaactgc
     1141 tatacaatgc agtgggccat tggcagcact ttcatcgagt gatacattgt ggtgcgataa
     1201 aacctgcatc gactttttcg acctct
//
LOCUS       DAORQA010000421         1222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_21617_length_1222_cov_3.580977, whole genome shotgun sequence.
ACCESSION   DAORQA010000421 DAORQA010000000
VERSION     DAORQA010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21617_length_1222_cov_3.580977"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1222)
                     /locus_tag="PK736_10390"
     CDS             complement(<1..>1222)
                     /locus_tag="PK736_10390"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRC33838.1"
                     /translation="CNTGGNVGIGTAAPDYKLKVSGDAMFNGVRVGKCSGGILSNTGV
                     GSGALNLNSTGERNTSIGFESMRSNTTGSFNSAFGTQALTANSTGSSNSAFGENALAA
                     NTASNNSAFGRKALFSNTTGTNNTAAGYFALQTNGLGSNNSAFGVNALSSNGKATNNT
                     AVGFSALTSNSEGSSNTATGSYALPLHDVGVGCTANGFQALKSDSTGNYNTGIGAFAD
                     IKFSNLINATSLGFEAIATGSNLVMLGNTTVMAVKAAGSFVIYSDVRFKKNIKENVPG
                     LDFINLLNPVTYNYNIHDLNKFIGANNELHTDKNIEAAILNKEKKVYSGFIAQDVEIA
                     AQKLGYDFSGVYKPQNESDPYGLSYAEFTVPLVKAVQELSKQNEELKNEISNLKSMVY
                     SNGDASSIRTQSSKQ"
BASE COUNT          333 a          261 c          213 g          415 t
ORIGIN      
        1 ttgttttgag ctttgagttc ttatgctcga tgcatcacca ttcgaatata ccatagattt
       61 taaattagaa atttcgtttt tcagttcttc gttttgcttc gataattctt gtacagcttt
      121 taccagcgga acagtaaact ctgcatagct taaaccataa ggatcgcttt cgttttgtgg
      181 tttatacaca ccactaaaat cgtaacccag tttttgtgct gcgatttcaa catcttgtgc
      241 tataaagccc gaataaactt ttttctcctt attgagtatt gctgcttcaa tatttttatc
      301 ggtatgtaat tcattatttg caccaataaa tttatttaaa tcatgaatgt tataattgta
      361 agtaacagga tttagtaaat ttataaagtc gagaccgggc acattttctt taatattctt
      421 tttaaagcgc acatcggaat aaatgacaaa actacctgct gcctttactg ccattacagt
      481 agtattgcca agcataacta ggttgcttcc tgtggctatg gcttcgaaac ccaatgatgt
      541 tgcatttatc aaattagaaa attttatatc ggcaaatgca ccaatacctg tattgtaatt
      601 tcctgtacta tcgcttttta atgcctgaaa accattggct gtgcatccta caccaacatc
      661 gtgcaatgga agtgcatatg accctgttgc tgtattacta ctgccttcac tatttgaagt
      721 gagtgcacta aaccccacag cagtattgtt tgtagctttt ccatttgacg ataaagcatt
      781 aacaccgaaa gccgaattgt tacttccaag tccatttgtt tgcaatgcaa aatatcccgc
      841 tgcagtattg ttggtgcctg tagtgtttga aaaaagtgcc tttcggccaa atgcagaatt
      901 attagaagct gtgtttgctg ccaaagcatt ttcgccaaat gcactgttgc ttgaacccgt
      961 tgagtttgct gtgagtgctt gtgtgccaaa tgcagaattg aaactcccgg tagtattgct
     1021 tcgcatcgat tcgaaaccta tggatgtatt gcgctcacca gtactattta aatttaatgc
     1081 tccacttcca actccggtat ttgataaaat accgccactg cacttaccca cacgcacacc
     1141 gttaaacatg gcatcgccac ttactttcaa tttgtagtcg ggggccgcgg ttcctatacc
     1201 aacattgcct ccggtgttac ac
//
LOCUS       DAORQA010000422         1208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_21900_length_1208_cov_4.639202, whole genome shotgun sequence.
ACCESSION   DAORQA010000422 DAORQA010000000
VERSION     DAORQA010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21900_length_1208_cov_4.639202"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1208)
                     /gene="aspS"
                     /locus_tag="PK736_10395"
     CDS             complement(<1..>1208)
                     /gene="aspS"
                     /locus_tag="PK736_10395"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRC33839.1"
                     /translation="MNSGEFYALPQSPQTFKQLLMVSGFDKYFQIVKCFRDEDLRADR
                     QPEFTQIDCEMSFIEQEDILNVFEGLVKTIFQNVKNISLSNVPRMTYDNAMKYYGNDK
                     PDIRFEMKFIELKSDTENYYVDNFATRFKVFDDAQYINAIVANGCSEYTRKQIDELTD
                     FVKRPQVGATGLVYIRVGNDGVCKSSVDKFFDADALNAIAKKCNAMPGDLILILAGPQ
                     QKTQKAMSELRLEMGNRLGLRDKNVFAPLWVLDFPLLEWNEESQRYHAMHHPFTSPKP
                     EDIPLLETQPGNVKANAYDMVINGVELGGGSIRIYDKQLQKKMFSILGFTDEQAQSQF
                     GFLMNAFEYGAPPHGGIAFGLDRFCSLLGGADNIRDFIAFPKNNSGRDVMIDAPSQID
                     DAQLKELRIN"
BASE COUNT          354 a          269 c          222 g          363 t
ORIGIN      
        1 catttatcct aagttcttta agttgagcat cgtcaatttg cgatggggca tcaatcatta
       61 catcgcggcc cgaattattt tttggaaaag caataaaatc gcgaatgtta tcggcaccac
      121 caagcagcga acaaaatcta tctaacccaa aagcaatacc accatgcgga ggggcgccat
      181 attcaaatgc attcatcaaa aaaccaaatt ggctttgtgc ctgttcatcg gtaaaaccta
      241 aaatagaaaa catctttttt tgcaattgct tatcgtaaat gcgaatagaa cctccgccca
      301 attctacacc atttatcacc atatcatacg cattggcttt tacatttccg ggctgtgttt
      361 cgagcaacgg aatatcctcg ggcttgggcg aagtaaaggg atgatgcatg gcatggtatc
      421 gttgcgattc ttcgttccac tcgagcaaag gaaaatcgag cacccatagg ggagcaaaaa
      481 catttttatc gcgcaagcca agcctgttgc ccatttcgag gcgaagctca ctcatggcct
      541 tttgtgtttt ttgttgtggc ccggcaagta tcaaaattaa atcgcccggc atggcattac
      601 atttttttgc aattgcattg agcgcatcgg catcaaaaaa tttatcgacc gatgatttac
      661 aaacgccatc gttacccaca cgaatatata ccaaacctgt agcacccact tgcggacgtt
      721 ttacaaaatc ggtaagctca tctatttgct tgcgtgtata ctcactgcat ccgtttgcaa
      781 caatggcatt tatatattgt gcatcgtcaa atactttgaa acgtgtggca aaattatcca
      841 cataataatt ttcggtatca cttttaagtt caataaattt catttcgaaa cgtatgtcgg
      901 gcttatcgtt accataatat ttcatggcat tgtcgtaagt catgcgtggc acattgctta
      961 gcgaaatatt ttttacattt tgaaaaatag tctttaccaa tccttcgaac acattgagaa
     1021 tatcttcctg ttcaataaaa ctcatttcac aatctatctg tgtaaattcc ggttggcggt
     1081 cggcacgcag gtcttcgtca cgaaagcatt ttacaatttg aaaatattta tcgaacccac
     1141 tcaccatcaa caattgctta aaagtttgtg gcgattgtgg cagggcataa aactcgccac
     1201 tgttcatg
//
LOCUS       DAORQA010000423         1191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_22271_length_1191_cov_6.753521, whole genome shotgun sequence.
ACCESSION   DAORQA010000423 DAORQA010000000
VERSION     DAORQA010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22271_length_1191_cov_6.753521"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PK736_10400"
     CDS             complement(<1..1128)
                     /locus_tag="PK736_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33840.1"
                     /translation="MKNTKFKLQGEEVQTTSNNFMKEFMPDATVSSYNISVNRGSPGN
                     ATEHTIELTDNDAVVVELENNIRWVYNRSDFVSKVQSQQPADRGSSSDELVLPLTWQD
                     ENQSRGLIGGALKLLGLKKVKNFVGEKLATAIAQKIEKNNQNELLICDKDFKLDKIFD
                     KATAKAGEGNYLLLIHGTASNTRGSFEGLATQNGGKTFEALFNQYPNRIISYEHKTLT
                     QSPITNVIDLVSKLPDDITLDMMSHSRGGLVGELIARLGCAEIDKCFTAREIEILSKN
                     EEAKSLLKEIELLHRVINKKQIKVNRFVRVACPAAGTILLSQRMDTMLNVMFNMMGRV
                     GGEVMGTISDGLEALVTAIVNEKNDFDTLPGIECMRPTSNFI"
BASE COUNT          297 a          252 c          231 g          411 t
ORIGIN      
        1 tataaaattg gatgtagggc gcatgcactc aatacctggc agtgtatcga aatcgttttt
       61 ttcatttact atcgcggtaa ccaatgcttc gagtccatct gaaatagtgc ccattacttc
      121 gcctcccaca cgacccatca tgttgaacat cacattgagc atggtgtcca ttcgttgcga
      181 tagcaaaata gtgcccgcgg cagggcaggc cacacgtaca aatcggttta cctttatttg
      241 ttttttattt attacacgat gcagcaattc tatttctttg agtaaagatt ttgcttcctc
      301 gtttttcgat agtatttcta tttcccttgc ggtaaaacat ttgtctattt cggcacaacc
      361 caatcgtgca atgagttcgc ctacaagccc accacgcgag tggctcatca tatccagtgt
      421 tatatcatcg ggcagtttcg atacaaggtc tatgacattg gtaatggggc tttgcgtaag
      481 cgttttgtgt tcgtacgaaa taatacggtt cggatattga ttaaataatg cttcaaaagt
      541 ttttcctcca ttttgtgttg caagcccttc gaatgatcca cgtgtattgg atgctgtacc
      601 atgtatgagt agcaaataat ttccttcgcc tgcttttgca gttgccttgt caaatatttt
      661 atcgagctta aaatctttat cgcaaatcaa taattcattc tgattatttt tttcgatttt
      721 ttgcgctatg gctgtagcaa gtttttcgcc cacaaagttt tttacttttt tcagaccaag
      781 caatttaagt gcgccaccta tcaatccgcg cgattggttt tcgtcctgcc atgtcagtgg
      841 caacacaagt tcatcgctgc tgcttcctct atctgccggt tgttgcgatt gcactttgga
      901 aacaaaatcg ctgcggttgt atacccaccg gatattattt tctaactcaa ctaccacggc
      961 atcgttgtcg gtaagttcta tggtgtgctc tgtggcatta cccggtgagc cacggtttac
     1021 acttatattg taactactta ccgttgcatc gggcataaac tctttcataa agttgttcga
     1081 agtagtttgt acttcttcgc cctgcaattt gaattttgta tttttcatat tatagaaagt
     1141 tagtgaaaaa aaagatagtg ttagttgcgg gtaaaactaa taggagaaaa t
//
LOCUS       DAORQA010000424         1164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_22841_length_1164_cov_4.495942, whole genome shotgun sequence.
ACCESSION   DAORQA010000424 DAORQA010000000
VERSION     DAORQA010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22841_length_1164_cov_4.495942"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1164
                     /locus_tag="PK736_10405"
     CDS             <1..>1164
                     /locus_tag="PK736_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HRC33841.1"
                     /translation="TKLTRAALEGSGLGTKEMDRSKNMFVLGLCYWMYNRELKNSIKF
                     LEEKFAKAPNILDSNVKLLKAGYAYGETTEAIETRYQINPAPMKAGVYRNIMGNQALA
                     IGLVAASKQSGLPIFYGTYPITPASDILHELSKHKNFGVRTFQAEDEIAAICSAIGAS
                     FGGCLGVTASSGPGIALKGEAMGLAVMLELPLVIINVQRGGPSTGLPTKTEQSDLLQA
                     MHGRNGEAPLPIIAACSPADCFNAALEACRIAVEYMTPVMLLSDGYIANGAEPWQFPQ
                     TADLPKITATFITEKNGGDKFLPYKRDENLSRPWAIPGTKSLEHRIGGLEKEHETGNI
                     NYENENHQFMVRMREAKVNKVADSIPLQKMDNGNEKGKLLVIGWGSTYGAIKM"
BASE COUNT          368 a          245 c          280 g          271 t
ORIGIN      
        1 ttacaaagct tacacgtgcc gcactcgaag gcagcggcct cggtacaaaa gaaatggacc
       61 gcagcaaaaa tatgtttgtg ctcggtttgt gctattggat gtacaatcgt gaattgaaga
      121 attctataaa atttcttgaa gaaaaatttg ccaaggcacc caacatttta gattcgaatg
      181 taaagttgct taaagccggc tatgcctatg gcgaaactac cgaagcaata gaaacacgat
      241 atcaaataaa ccccgcaccc atgaaagccg gtgtgtaccg caacattatg ggcaatcagg
      301 cattggccat aggtttggtg gcagcatcaa agcaaagtgg cctgcccatt ttttatggca
      361 catatcccat cactccggca tcggatattt tgcacgaatt atcgaaacat aaaaattttg
      421 gtgtgcgtac atttcaggcc gaagacgaaa tagccgccat atgttcggcc ataggtgcaa
      481 gttttggggg gtgcctggga gtgactgcat catcgggacc gggcattgcg ctcaaaggcg
      541 aagcaatggg actggcagta atgcttgagt tgccactggt aataataaat gtacaacgcg
      601 gtggaccatc aacaggcttg cctacaaaaa cggaacagag cgatttgttg caagccatgc
      661 atggacgaaa tggcgaagcg ccactgccaa taattgcagc atgttctcca gccgattgtt
      721 ttaatgccgc actcgaagca tgccgcattg cagtggaata catgactcct gtaatgttgc
      781 ttagcgatgg ttacattgcc aatggtgccg agccatggca atttccgcaa acagcagact
      841 taccaaaaat aactgccact tttataacag aaaaaaatgg tggcgataaa tttttgcctt
      901 acaagcgcga tgaaaattta tcgcgtccct gggctattcc cggcaccaaa agtttggagc
      961 atcgcattgg cggattagag aaggaacacg aaacaggaaa cattaattac gaaaacgaaa
     1021 atcaccagtt tatggtgcgc atgcgcgaag ccaaagtaaa caaagtggcc gatagtattc
     1081 cactgcaaaa aatggataat ggaaacgaaa aaggaaaatt acttgtgata ggttggggca
     1141 gtacttatgg cgcaataaaa atgg
//
LOCUS       DAORQA010000425         1150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_23177_length_1150_cov_3.463014, whole genome shotgun sequence.
ACCESSION   DAORQA010000425 DAORQA010000000
VERSION     DAORQA010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23177_length_1150_cov_3.463014"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PK736_10410"
     CDS             complement(<1..938)
                     /locus_tag="PK736_10410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRC33842.1"
                     /translation="MEIGIDSFAASPSSLQISPSQVINELIERIVLADEVGLNVFGIG
                     EHHRKEFLDSAPSMILAAAAARTKNIILTSAVTVLSAADPVRVFQNFATLDLISNGRA
                     EIVVGRGSFIESFPLFGLNLNDYDELFTEKLELLLKIRDNEIVNWSGKFRPALINQAI
                     YPRPVQKQLPIWLGVGGTPASFVRAGELGMPLMVAIIGGETHRFKPLIDLYKNAGAKA
                     GHTADKLKVGLHSLGYVAATKDEAVEDYYPGYAEMFTRIGKERGYAPVSKMHFDSQNG
                     KHGALLVGDPQEIAEKIIRHSEALGGISRFSFQMDAG"
     gene            complement(994..>1150)
                     /locus_tag="PK736_10415"
     CDS             complement(994..>1150)
                     /locus_tag="PK736_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRC33843.1"
                     /translation="GYNQTDLANIVGLKSRASELLNKKRKLTLEMIRLINQSLKIPTD
                     VLIQAYH"
BASE COUNT          320 a          243 c          220 g          367 t
ORIGIN      
        1 ccggcatcca tctgaaacga aaaacgtgat atgcctccga gtgcctcact gtggcgaatt
       61 attttttcag ctatttcttg tgggtcgcca acaagtaatg ccccatgttt tccattttga
      121 gaatcgaagt gcatttttga aaccggtgca tagccacgct ctttacctat gcgtgtaaac
      181 atttcggcat agccgggata atagtcttca acagcttcgt cttttgtagc ggctacataa
      241 ccaagcgaat gtaaaccaac tttaagttta tcagctgtat ggcctgcttt tgcaccggca
      301 tttttgtata aatctatcaa tggtttgaat cgatgcgttt cgccaccaat gatggcaacc
      361 ataagcggca tacccaactc gcccgcgcga acgaatgatg caggtgtgcc gccaacacct
      421 aaccaaatgg gaagttgttt ttgcaccgga cgtggataga ttgcctgatt gataagcgca
      481 ggacgaaatt tacccgacca gtttacaatt tcgttgtcac gaatttttaa tagcagttct
      541 aatttttcgg taaaaagttc atcgtagtca ttcaggttta agccaaataa cggaaacgat
      601 tcgataaacg aaccacgccc tacaactatt tcggccctgc catttgatat taagtcgagt
      661 gttgcaaaat tttgaaaaac acgaaccggg tcggcagcac ttaatactgt tacagcactt
      721 gtgagtataa tattttttgt acgggcagct gccgcagcta aaatcattga tggtgcagaa
      781 tcaagaaatt cttttcgatg gtgttcgcca attccaaata catttaggcc aacctcatcg
      841 gcaagtacta tgcgttcaat aagttcatta attacttgag aagggcttat ttgcaaactc
      901 gatggcgatg ctgcaaaact atctattcct atttccattt ttttccatgc attattttaa
      961 aaatctattt tgtgttatat caaagcgtgt cttttaatgg taagcttgaa tcaaaacatc
     1021 agttggtatt tttaaacttt ggtttatcag tcgaatcatt tctaatgtca gctttctttt
     1081 tttatttagc aattcacttg cacgactttt taaaccaacg atgttagcca aatcggtttg
     1141 gttgtaaccc
//
LOCUS       DAORQA010000426         1145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_23290_length_1145_cov_4.808257, whole genome shotgun sequence.
ACCESSION   DAORQA010000426 DAORQA010000000
VERSION     DAORQA010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23290_length_1145_cov_4.808257"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1145)
                     /locus_tag="PK736_10420"
     CDS             complement(<1..>1145)
                     /locus_tag="PK736_10420"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HRC33844.1"
                     /translation="LNKLPKTLFSKTERKFINEELKNDRKMIVINQFKRYVFIQILED
                     KKGYIHQSEAMRKAGDSIAAQLNKQKQNTVTIMAGNRSGAALAMAEGMALGNYQFIQF
                     RKDKAKEKNALSTINVVCDNVTPKDISMLNITVDAVCHARDLVNIPNSHQTATQLATS
                     FQDLGKKAGFEVEVWNMAKISSMKMGGLLAVNVGSPEPPTFTVMEYKPANAVNKKPYV
                     LVGKGVVYDTGGLSLKPTANSMDLMKCDMAGSAVVACTMYAIAKAKLPVHLIGLTPAT
                     DNRPGGNAFAPGDIIHMMNGATVEMLNSDAEGRMILADALHHAKQYKPELVCEFSTLT
                     GAAAAAIGHYGIVCMGTAKDEVKDKLKLAGNNVFERLVEFPFWEEYD"
BASE COUNT          275 a          274 c          225 g          371 t
ORIGIN      
        1 tcatcgtact cttcccaaaa cggaaattct accaaccgct caaacacatt attaccggca
       61 agttttaatt tgtctttcac ttcgtctttt gctgtgccca tacatacaat gccgtaatgc
      121 cctatggctg ctgctgctgc gcccgtgagg gtactaaact cacacaccaa ttcgggttta
      181 tattgtttgg catggtgcaa tgcatcggca agtatcatgc ggccttcggc atcgctgttg
      241 agcatttcta cggtggcacc attcatcatg tgtataatat cgccaggtgc aaatgcattg
      301 cctccggggc gattgtcggt ggcgggagta agtcctatta aatgaactgg caatttagct
      361 tttgcaattg catacattgt acaagccaca actgcactgc cggccatatc acatttcata
      421 aggtccatac tattggctgt aggtttcaaa ctcaagccgc ctgtatcgta caccacaccc
      481 ttgcccacaa gcacataagg ttttttattt actgcattgg caggtttata ttccatcact
      541 gtaaacgtag gtggctccgg gctgccaaca tttacagcaa gcaagccgcc cattttcatc
      601 gaacttattt ttgccatatt ccacacctcc acttcgaagc cggccttctt gccaaggtcc
      661 tgaaacgagg ttgcaagctg agttgctgtt tgatgactat tgggtatatt caccaaatcg
      721 cgcgcatggc acacagcatc taccgtaatg tttagcatgc ttatatcttt gggagttaca
      781 ttatcgcaca caacatttat agtagacagg gcattttttt ctttcgcttt atctttgcga
      841 aattgtataa actgataatt gccaagcgcc attccctcgg ccatagcaag ggctgcacca
      901 ctgcgattgc cggccataat tgttactgta ttttgctttt gcttgttgag ctgtgcagca
      961 atgctatcgc ctgctttacg catggcttcg ctttggtgta tatagccctt tttatcttcg
     1021 agtatttgta taaaaacata acgcttaaat tgatttataa caatcatctt acggtcgttt
     1081 ttcaactctt cgtttataaa ctttcgctct gttttgctga ataatgtttt tggaagttta
     1141 ttaag
//
LOCUS       DAORQA010000427         1144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_23302_length_1144_cov_6.130395, whole genome shotgun sequence.
ACCESSION   DAORQA010000427 DAORQA010000000
VERSION     DAORQA010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23302_length_1144_cov_6.130395"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1144)
                     /locus_tag="PK736_10425"
     CDS             complement(<1..>1144)
                     /locus_tag="PK736_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRC33845.1"
                     /translation="NEELFPIDVKRRIFQKLNEAVAFESFLHSKFVGQKRFSLEGCET
                     LIPALDVVIDYGADLGLKEYCIGMAHRGRLNVLSNIMQKTREQIFTEFEGKEFDITGF
                     DGDVKYHLGYSTDITTTKGHQVHLSLAPNPSHLEAVGPVVQGIVRSKIDNQNGNENDI
                     AAILIHGDAAIAGQGIVYEVTQMSMLDGYRTGGTIHLVVNNQIGFTTNYLDARSSTYC
                     TDAAKVTKSPVFHVNGDDVEALVYTIKLAIEYRQKYHRDVFVDILGYRKYGHNEGDEP
                     RFTQPLLYKAIAVHPNPREIYNAKLLAQGDVEASLAKEMEKEFKAMLQVDLDKARKNE
                     KTKFNVDNEGAWKGLRIPSDKEIFTTADTKVALDILLKIGDSISTLP"
BASE COUNT          286 a          271 c          219 g          368 t
ORIGIN      
        1 ggtaatgtgc ttatgctgtc gccaattttt aataaaatat cgagcgctac ttttgtatct
       61 gctgttgtaa atatttcttt gtcagatgga attcgcaaac ctttccatgc accttcgtta
      121 tcaacattaa attttgtttt ttcattcttg cgtgctttat ccaaatcaac ctgcagcatg
      181 gctttaaatt ccttttccat ttccttggca agcgatgctt caacatcgcc ctgcgcaagc
      241 aatttggcat tatatatttc gcgcggattg ggatgcacag caattgcttt gtataaaagt
      301 ggttgtgtaa agcgcggttc atcgccctcg ttgtggccat actttctgta tcctaaaata
      361 tctacaaaca catcgcgatg atatttttgt cgatactcaa tcgcaagttt tatggtgtac
      421 accaatgcct ccacatcgtc accatttaca tgaaacacag ggctcttggt aaccttggct
      481 gcatcggtac aataggtact gctgcgtgca tcaagatagt tggttgtaaa tccaatttga
      541 ttattcacca ccaaatgtat ggtgccaccg gtgcggtaac catcgagcat gctcatttgt
      601 gtaacctcgt atacaatgcc ttgcccggca attgcggcat cgccatgtat aagtatggcc
      661 gcaatatcat tttcgtttcc gttttggtta tcgattttcg atctcactat tccttgcact
      721 accgggccca ctgcctcgag gtgcgatgga ttgggtgcta aactcaaatg cacctgatgt
      781 ccttttgtgg tggtaatatc cgttgaataa cccaggtgat attttacatc accatcgaaa
      841 ccggtaatgt caaattcttt gccttcaaat tctgtaaata tttgctcacg cgttttttgc
      901 atgatgttgc tcaacacatt caagcgccca cgatgcgcca tgccaataca atactctttc
      961 aagcctaagt cggcaccgta atcaatcacc acatccaatg caggaatgag ggtttcgcag
     1021 ccttcgagcg aaaaacgctt ttgccctaca aacttgctgt gcaaaaaact ttcgaaggca
     1081 acggcttcgt tcagcttttg aaatattctc cgctttacat caatgggaaa taattcttcg
     1141 ttgc
//
LOCUS       DAORQA010000428         1138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_23412_length_1138_cov_10.383195, whole genome shotgun
            sequence.
ACCESSION   DAORQA010000428 DAORQA010000000
VERSION     DAORQA010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23412_length_1138_cov_10.383195"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="PK736_10430"
     CDS             <1..1118
                     /locus_tag="PK736_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC33846.1"
                     /translation="ISGSTASNNGDVSGNHSPGINDMWLLKTDTQGVYAWNKCVGGAS
                     NDDGYACLYANNFGYFVGGRSSSIDGDITAPLGLSDFVVAKTAPDPPPVAIYASINFI
                     GTVCPGLNFNVSCSVIGTVVNGNIYRAYLSDSAGSFTTETLIGSLTSNLSSAIIPCVI
                     PKTAKGTNYLIRIKSSNTPYTTNPTSTPFAVACKAPGNLVAVNVTATDAYLEWDDASL
                     CTAEYQIYSRVFGTTAWYVANIPFSDVVLTGLLSSTKYQWKVRARCATSPNVFSPFTA
                     TKTFTTFSPRQSNEETIENENTEIINVSPNVFMINFSDKTEAVNIDVIDLNGRLMMQK
                     TTNDNITQIDLNNFNKGLYVIRCYTNNYSFNGKILVE"
BASE COUNT          359 a          207 c          230 g          342 t
ORIGIN      
        1 ttataagtgg atccaccgct agtaataatg gcgatgtgtc gggcaatcat tctccgggga
       61 taaatgacat gtggctttta aagaccgata cgcaaggtgt ttatgcctgg aataaatgtg
      121 taggaggggc aagcaacgat gatgggtacg cttgcttgta tgcaaataac tttggatatt
      181 ttgttggtgg ccgttcatcg tctatcgatg gtgatattac cgcaccgttg ggcttgtccg
      241 attttgttgt agcaaaaaca gcacccgatc caccacctgt tgcgatatat gcaagcatta
      301 attttatcgg aacagtttgc cccggtttaa actttaatgt gtcttgcagt gttattggca
      361 cagttgtaaa cgggaatatt tatcgtgctt atttatcgga ttcggcaggg agttttacca
      421 ccgaaacatt aataggttct ttgacaagta atttatcgag tgccatcatt ccttgcgtaa
      481 ttccaaaaac agctaagggc acaaattatt taatacgaat aaaaagcagc aatactccat
      541 acactacaaa tcctacttca actccatttg ccgtagcatg taaagcacca ggcaatctgg
      601 tagcggtgaa tgtaactgca acagatgcat acctggaatg ggacgatgcc tcactgtgta
      661 ctgccgaata tcaaatatat agtagagtgt ttggtacaac tgcgtggtat gttgccaata
      721 tcccttttag cgatgtagta ttgactggtt tactcagctc cacaaaatat caatggaaag
      781 taagggcaag gtgtgctacc agcccaaatg ttttttcacc attcacagca acaaaaactt
      841 tcaccacatt ttctccacgt caatccaatg aagaaactat tgaaaatgaa aacacagaaa
      901 taataaatgt atcgcccaat gtttttatga taaacttttc ggataaaacc gaagctgtaa
      961 atatcgatgt tatagattta aatggtcggc ttatgatgca gaaaacaacc aatgataata
     1021 ttacacaaat agatttgaat aatttcaaca agggtctgta tgttattcgt tgctatacga
     1081 ataattattc tttcaatgga aaaatattgg tagagtaata tgataatcag ttgatatg
//
LOCUS       DAORQA010000429         1135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_23486_length_1135_cov_6.117593, whole genome shotgun sequence.
ACCESSION   DAORQA010000429 DAORQA010000000
VERSION     DAORQA010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23486_length_1135_cov_6.117593"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PK736_10435"
     CDS             <1..869
                     /locus_tag="PK736_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33847.1"
                     /translation="KQTQPCAGITAPVWYSNSTALFTCPGYKVGIGTYDPKGPLHIFH
                     NDANNSLIIDRASNTPGETKSQISFQYAGVEKWSTGINAFGTNLLQNDYYVYGGTNPR
                     VQFYIAQNGNILCGNTSAWLPNALNNDCQFHIVSDVDRKSGLCVEATGLTNAAVSNGL
                     HVMVDDAVIKAILVTNEDAPNGCKDVFRVYGSGYVEAKDVKVFVAGWCDNVFDKQYPL
                     LSLEMVEQYIAQNKHLPGVPSEAEVVANGLSVADMFTIQMKKIEELTLYMVEQNKKIT
                     ELQNQIQILKNK"
     gene            881..>1135
                     /locus_tag="PK736_10440"
     CDS             881..>1135
                     /locus_tag="PK736_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33848.1"
                     /translation="MKNKLFKIFVLFIFTTMQCKSQTAFTYPGSNIQNVDWNSWSAHT
                     INYAVPGKVTATPLPAQPVYIHETAKVDITSSTEVHMADGF"
BASE COUNT          392 a          189 c          234 g          320 t
ORIGIN      
        1 taaaacaaac acaaccttgt gcaggcatca ccgctcctgt ttggtatagc aattcaacag
       61 cattgtttac ttgccctgga tataaagtag gaataggaac ttatgatcca aagggcccgt
      121 tacatatatt ccacaacgat gcgaataatt ctttaattat tgacagggca agcaatacac
      181 cgggtgaaac aaaaagtcaa atttcatttc aatatgccgg tgttgaaaaa tggagtacag
      241 gaataaatgc atttggaaca aatcttttgc aaaacgatta ttatgtttat ggtggtacaa
      301 accccagggt tcaattttac attgcacaga atggaaatat actttgtggt aatacttctg
      361 catggttgcc aaatgcgtta aacaacgatt gtcaatttca tattgttagc gatgttgaca
      421 gaaaatcagg cttgtgtgtt gaggcaaccg ggcttacaaa tgcagccgtg agtaatggct
      481 tgcatgtgat ggtagatgat gccgttataa aagccatttt agttacaaat gaagatgcac
      541 caaatggatg caaggatgtg tttagggttt atggtagtgg ttatgtagaa gccaaagatg
      601 taaaagtatt tgttgctgga tggtgcgata atgtattcga taaacaatat ccattgcttt
      661 cgctcgaaat ggtggaacaa tatatagcgc aaaataaaca tttgccgggt gtgccaagcg
      721 aagccgaagt agtggctaat ggtttgagtg ttgcagatat gtttacaata caaatgaaaa
      781 aaattgaaga acttacgttg tacatggtgg aacaaaataa aaaaattacc gaactgcaaa
      841 atcaaatcca aattttgaaa aacaaataaa ttgcaccgcc atgaaaaata aattatttaa
      901 aatatttgtg ctttttattt ttacaactat gcaatgtaaa agtcaaacag cctttacata
      961 tcccggtagc aatatacaaa atgtggattg gaattcgtgg agtgcccaca ctataaatta
     1021 tgctgtgcca ggcaaagtga cagcaactcc gctgccagcg caaccagtat atatacacga
     1081 aactgcaaaa gtagatatta cctcatccac cgaggtacac atggctgatg gtttt
//
LOCUS       DAORQA010000430         1120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_23847_length_1120_cov_7.498592, whole genome shotgun sequence.
ACCESSION   DAORQA010000430 DAORQA010000000
VERSION     DAORQA010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23847_length_1120_cov_7.498592"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1120)
                     /locus_tag="PK736_10445"
     CDS             complement(<1..>1120)
                     /locus_tag="PK736_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRC33849.1"
                     /translation="TQKTVVIKNTTVWTNESDGNLINTDVLIKNGKIALVGKNINEPG
                     AEVIDGTEKHLTAGIIDEHSHIAVSGAVNECTQSVTAEVRIADVIDCEDVNIYRQLAG
                     GVTSSHLLHGSCNPVGGQTQLIKLRYGLSSEELKFANWPGFIKFALGENVKQSNAGDN
                     NTSRYPQTRMGVEQTYYNAFTRALDYQKAIKSFNALSTADRLKAKPVRKDLELDALAE
                     IINGKRFITCHSYVQSEINMLMHVADSFHFQVNTFTHILEGYKVADKMKAHGAGASSF
                     SDWWAYKYEVYEAIPYNGKILHDVGVTVAYNSDDAEMARRLNQEAAKAVKYGGVSEQE
                     AWKFVTLNPAKLLHVDDKVGSIKVGKDADVVLWSDNPLS"
BASE COUNT          287 a          257 c          202 g          374 t
ORIGIN      
        1 gataatggat tatcactcca taaaactaca tcggcatctt tccccacttt tatacttcct
       61 actttatcat caacatgcaa taattttgca gggttaagag ttacaaattt ccatgcctcc
      121 tgttcactta cgccaccgta cttcacagcc tttgccgctt cctggttcag ccttcgggcc
      181 atctccgcat cgtccgaatt ataggctact gttacaccaa catcatgcaa tattttaccg
      241 ttgtatggaa tagcctcgta cacttcgtat ttatatgccc accaatcgct gaatgacgat
      301 gccccggcac catgtgcttt cattttatcg gcaactttgt atccttcaag tatatgtgta
      361 aaagtattta cctgaaaatg aaaactatcg gcaacatgca tcagcatatt tatttcgctt
      421 tgcacatagg agtggcaagt aataaatctt ttgccattaa taatctctgc caatgcatca
      481 agttctaaat ctttgcgcac cggttttgct tttaatctat ctgcagtgga aagtgcattg
      541 aatgatttta tcgctttttg ataatcgagc gcacgggtaa atgcattgta ataagtttgt
      601 tctactccca tgcgggtttg cggataacgc gatgtgttgt tatcgcctgc attactttgc
      661 tttacatttt cgccaagggc aaattttata aagcccggcc aattggcaaa tttcaattct
      721 tcggaactta atccgtaccg aagttttata agttgtgttt gccccccaac agggttgcac
      781 gaaccatgaa gtaaatgcga tgatgttacc ccgcctgcaa gctgacgata aatattcacg
      841 tcttcgcaat cgattacatc tgcaatgcga acttcggcag ttaccgattg ggtgcattca
      901 tttactgccc ccgaaacggc aatgtgcgag tgttcatcga taatgccggc tgttaggtgt
      961 ttttctgtac catcaataac ttcggcacca ggctcattta tatttttacc taccagtgct
     1021 attttaccat ttttaataag cacatcggtg ttgattaaat taccatcact ttcattggtc
     1081 catacagtgg tgtttttaat aacaactgtt ttttgtgtgg
//
LOCUS       DAORQA010000431         1108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_24136_length_1108_cov_5.470085, whole genome shotgun sequence.
ACCESSION   DAORQA010000431 DAORQA010000000
VERSION     DAORQA010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24136_length_1108_cov_5.470085"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..>1108)
                     /locus_tag="PK736_10450"
     CDS             complement(1..>1108)
                     /locus_tag="PK736_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33850.1"
                     /translation="YTAIVKFILFGHCNAIFYLDCIVFQRKHFTFGNYTMKTKLILIT
                     LLALGVAGVFFTSCNNDLDIVAPYKDTTVVYCLIDLNDTVHYVRIHKAFLGADNAYEM
                     AQYTDSFYHKNVLSVKLERWKNNQLYGVINFTRDSSIAKDTGLFANTPNIIYKSESCN
                     LLSADSKYRLVVRNSETGSVVTSVTQMIQPTLIANPSSGTINLYPGPFVIRIASGKYG
                     KIYDIKARFNYIEQQKNNPNITENKSVVWNITQVTTVSDAGSELINYAVGKDDFYQFC
                     KSSIKVDNNVVRYSGKLDFYFTAGDQAMYEYLRINNASGVNASNGLLFTNIENGLGIF
                     ASRRTEISLGKSMAGNSIGYLISGPLTSQLGFIQ"
BASE COUNT          339 a          214 c          193 g          362 t
ORIGIN      
        1 ttattgtatg aagccaagtt gcgatgttag tggtccgctt atgaggtaac caatcgaatt
       61 tcctgccatc gattttccta aagaaatttc tgtacgtctg ctggcaaata tgcctagtcc
      121 attttcgata tttgtaaata gcaatccatt acttgcattt acgccactgg cattattgat
      181 tcgcaaatac tcatacatgg cctggtcacc tgcggtaaaa taaaaatcga gtttgccact
      241 ataccgaact acgttattgt ctactttgat tgatgattta caaaactggt aaaagtcatc
      301 tttgcctaca gcgtaattta tcaactcgga accggcatcg ctaaccgttg ttacttgtgt
      361 aatattccac accacacttt tgttttcggt aatgttggga ttattttttt gttgctctat
      421 ataattgaac cgtgctttaa tatcatagat tttgccatac ttacccgaag caatacgaat
      481 aacaaaaggg cccggataaa gattgatagt gcccgaactg ggatttgcta ttaatgtagg
      541 ttgaatcatt tgtgttacac tagttaccac cgatccggtt tcactattgc gaacaactaa
      601 cctgtatttg ctatcggccg ataataaatt acacgattcg gatttgtaaa taatgttagg
      661 cgtattggca aacaatcctg tatcttttgc aatactgcta tcgcgtgtaa aattaatcac
      721 cccatatagt tgattgtttt tccatcgttc gagttttacc gacagcacat ttttatgata
      781 aaacgaatcg gtatactgcg ccatttcata agcattatca gcgcccaaaa aagctttgtg
      841 aatgcgcaca tagtgaacag tgtcgttcaa gtcgattagg cagtatacca ctgtggtatc
      901 tttatagggt gcaacaatat ctaaatcgtt attgcatgaa gtaaaaaaaa cacccgccac
      961 accaagcgct agcagtgtta ttaaaatcaa ttttgttttc atagtataat tgccaaaagt
     1021 aaaatgtttt ctttggaaaa cgatgcaatc caaataaaaa attgcattgc aatgaccaaa
     1081 tagaataaat tttacaattg ctgtatac
//
LOCUS       DAORQA010000432         1106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_24191_length_1106_cov_5.242626, whole genome shotgun sequence.
ACCESSION   DAORQA010000432 DAORQA010000000
VERSION     DAORQA010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24191_length_1106_cov_5.242626"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..>1106)
                     /locus_tag="PK736_10455"
     CDS             complement(184..>1106)
                     /locus_tag="PK736_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRC33851.1"
                     /translation="INVCAKNFGDRVKNWMVLNEPLVFTGAGYFLGIHAPGHRDFRFF
                     LKAAHHAALSQSIGSHILKNEVKNSMVGSTFSCSYITPYSFTNHDENAALRADALLNR
                     FFIEAAMGKGYPVEHLEFMKKIYNYVLPGDEQLLKAQFDFVGIQNYTREVIKHTCWIP
                     LIRARIIPANKRKVAHTAMNWEIYPESIYHMLKQFNSICDGLPLIITENGAAFDDKLE
                     DGKIIDDYRIHYLQRNIEQIHRAKTEGVNILGCFIWSLFDNFEWEKGFKPRFGLVHVD
                     FDTHLRTAKKSALWYRDFLANQNNSITVRS"
BASE COUNT          365 a          196 c          191 g          354 t
ORIGIN      
        1 gtgtaagcac acttattgga tttgtatcgg tgatatggtt cgagggtgtg aagtggacaa
       61 acagattgaa aaaaaagatt tgaaattcaa taatctcttt ttaattttat cagtgaattt
      121 aataaattgt aagttcaacg aataagtgca acagcattat acttgaataa tcaaattcga
      181 aaattaagag cgcactgtaa tcgaattgtt ttgattggct aaaaaatcac gataccacag
      241 tgccgatttt tttgctgttc gaaggtgtgt atcgaagtca acatgcacaa gaccaaaacg
      301 gggcttaaat cctttttccc attcaaaatt atcgaataac gaccaaataa aacaaccaag
      361 aatattaacg ccttcagttt ttgcgcgatg aatttgttct atatttcttt gcaaatagtg
      421 aatgcgataa tcgtcaatga ttttgccatc ttcaagcttg tcgtcaaaag ctgcaccgtt
      481 ctcggtaatt attaaaggca acccatcaca tattgaattg aactgcttta gcatatgata
      541 aattgactcg ggatatattt cccagttcat agccgtatgt gccactttgc gtttatttgc
      601 cggtattata cgggctctta ttaatggaat ccaacaagta tgcttaataa cttcgcgggt
      661 ataattttgt ataccaacaa aatcaaattg tgctttcaat aattgttcat cgccaggtaa
      721 aacatagttg taaatttttt tcataaactc taaatgttct accggatagc ctttacccat
      781 agcagcttca ataaaaaaac ggttgagcaa tgcatcggca cgcaatgccg cgttttcatc
      841 atgatttgta aaagagtaag gagtgatata agagcatgaa aaagtagaac ctaccataga
      901 attttttact tcatttttta aaatatgaga tcctattgat tgagacaatg ctgcatggtg
      961 agcagctttt aaaaaaaatc gaaagtcgcg atgacctggc gcatgtatac ctaaaaaata
     1021 tcctgcccct gtaaatacta aaggttcatt tagcaccatc cagtttttta ccctatcgcc
     1081 aaaatttttt gcgcacacat ttatgt
//
LOCUS       DAORQA010000433         1077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_24936_length_1077_cov_2.529354, whole genome shotgun sequence.
ACCESSION   DAORQA010000433 DAORQA010000000
VERSION     DAORQA010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24936_length_1077_cov_2.529354"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PK736_10460"
     CDS             complement(<1..221)
                     /locus_tag="PK736_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRC33852.1"
                     /translation="MKEMKVEKVFNLSTKQQGDVWNLFFDSQKRLWVGGYPDMGLMMI
                     ENDTLLDYSERYKLENQTILAMVEDNNNN"
     gene            290..790
                     /locus_tag="PK736_10465"
     CDS             290..790
                     /locus_tag="PK736_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33853.1"
                     /translation="MREANGLSSDFIYTLGFDSDSNFLWVGNNQGLDKVTINAYLKDG
                     VSEISHFGKEEGFFPLETNSYGFYRDTDNSIWFGTVNGLVHYNALPGSQHKNKNFTYI
                     ESIKIDYTDTTLTDKVVLPYYCNNLSFNFISIDFINPNKVTYSYKLTGLTNEWSPFIV
                     SKTVTG"
     gene            747..>1077
                     /locus_tag="PK736_10470"
     CDS             747..>1077
                     /locus_tag="PK736_10470"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRC33854.1"
                     /translation="MNGRHSSFPKLSPGKYSFDVRSRNNEGVISTTAAPFSFTIRPPF
                     WRTWWFYSLSALLVTLTIYVLVRRRVKSIRQKSLMKQQIIESELKALRSQINPHFLFN
                     SLNSIQAF"
BASE COUNT          323 a          224 c          184 g          346 t
ORIGIN      
        1 atattgttat tattatcttc aaccatagca agtatggttt gattttctaa tttatatctc
       61 tcagagtaat cgagcaaggt atcattttct atcatcatca aacccatatc gggatagccg
      121 cccacccaca ggcgtttttg tgaatcgaaa aataaattcc atacatcgcc ttgttgcttc
      181 gttgataaat taaaaacctt ttccactttc atttccttca tgcgcactac tttatttttg
      241 ccgcactttc gatggtgttt atattataca acgcaacaaa gtaaaatatt tgcgtgaggc
      301 caatgggctc agctccgatt ttatttatac gcttggtttc gatagcgaca gtaatttttt
      361 atgggttgga aataatcagg gactcgataa agtaaccata aatgcttatc tcaaagatgg
      421 ggtttccgaa atttcacact ttggaaagga agaaggcttt tttcctttag agacaaattc
      481 ctatggcttt tatcgcgata ccgacaactc catttggttt ggcactgtaa acggattggt
      541 acactataat gctttgccgg gcagccagca taaaaacaaa aactttacct acatcgaatc
      601 gataaaaatt gattatacag atactactct caccgacaaa gtagtgctgc cctattattg
      661 caacaaccta tcgttcaatt ttatcagcat cgattttatt aatcccaaca aggttactta
      721 ctcatataag ctcacagggt tgactaatga atggtcgcca ttcatcgttt ccaaaactgt
      781 caccgggtaa atattcgttt gatgtacgaa gccgcaacaa cgaaggtgtt ataagcacta
      841 cggctgcccc attttcattt acaattcgtc ctcctttttg gcgcacgtgg tggttctatt
      901 ctttatcggc attattggta acccttacta tatacgtttt ggtaaggcgc agggtaaaat
      961 ctatacggca aaaatcgctg atgaaacaac aaataataga atcggaatta aaagcgctgc
     1021 ggtcgcaaat aaatccacat ttcttattta actcgctcaa tagcattcaa gcattta
//
LOCUS       DAORQA010000434         1025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5381
            NODE_26368_length_1025_cov_6.810309, whole genome shotgun sequence.
ACCESSION   DAORQA010000434 DAORQA010000000
VERSION     DAORQA010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867598
            Sequence Read Archive: SRR5738867
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:18:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,051
            CDSs (with protein)               :: 2,051
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 12 of 24
            Pseudo Genes (incomplete)         :: 12 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26368_length_1025_cov_6.810309"
                     /isolate="AMR_MDS_5381"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..>1025
                     /locus_tag="PK736_10475"
     CDS             40..>1025
                     /locus_tag="PK736_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC33855.1"
                     /translation="MQKFTKVLILFLAICNFCFAQITIDENDLMNAGDTFRVSIASNQ
                     QYQTSVLTQTGANQTWDFSTLVPISQTIDTFIDVATANLLYLVTYSNFGFNANRANIV
                     QQNTGGINLPLPVTLDNPATFYYKGASNFRSPGTGVELSGLATALPYGAEKDTIYKFP
                     LNFNDTDSGAVAASLSIPGLGSYNTTQKRLWLADGWGTLITPYGTFDVLRVKAKLTGR
                     DSLADTSGFGFAIPRPETMVYRWLGKNQGIPLLEIQTTKNFNVVAISSVRYRDNFTPV
                     LSSIVQNENLYNVGLYPNPCKGDYFALRFAMQHAADITVSVYALTGKLINTKT"
BASE COUNT          306 a          222 c          196 g          301 t
ORIGIN      
        1 cttttctgta tgtttacaaa aaattaattc actatatcca tgcaaaaatt taccaaagtt
       61 ttaatattgt ttcttgcaat ttgcaatttc tgttttgctc aaattaccat tgacgaaaac
      121 gaccttatga atgcaggtga cactttcagg gtaagcattg ccagtaatca acaataccaa
      181 acatcggtgc ttacacaaac aggagcaaac caaacatggg atttttcgac acttgtacct
      241 atcagccaaa caattgatac atttattgat gtggcaacag ccaatttgct ttatctggtt
      301 acatacagca actttggttt caatgctaac cgtgctaaca tagtgcaaca aaataccgga
      361 ggaattaatt taccactgcc tgttacactc gataatccgg caacgtttta ttacaaaggt
      421 gcctcaaact ttcgtagccc cggcacaggt gttgagctta gcggtttggc aacggcatta
      481 ccttacggtg ccgaaaagga tacaatttat aagttcccat taaattttaa tgataccgat
      541 tcgggcgcag ttgccgcatc cttaagtata cccggtttgg gttcgtacaa taccactcaa
      601 aagcgattgt ggcttgccga tggctggggc acactcatta cgccatacgg caccttcgat
      661 gtgttgcgcg taaaagcaaa acttaccggg cgcgattcat tggccgatac aagcgggttt
      721 ggttttgcaa taccacgccc cgaaacgatg gtttatcgtt ggcttggaaa aaatcaaggg
      781 attccgttat tagaaataca aactacaaaa aatttcaacg ttgtagctat ttcttcggtg
      841 cgttaccgcg ataattttac gcctgtgttg tcatcaatag ttcaaaatga aaacctttat
      901 aatgttggtt tgtatcctaa cccctgcaaa ggcgattact ttgctttacg atttgccatg
      961 cagcatgctg ccgatataac cgtaagtgta tacgcactta cagggaaatt gataaacacc
     1021 aaaac
//
